LOCUS       DAOQHP010000001        23175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_976_length_23175_cov_3.795502, whole genome shotgun sequence.
ACCESSION   DAOQHP010000001 DAOQHP010000000
VERSION     DAOQHP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 23175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23175
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_23175_cov_3.795502"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLY81_00005"
     CDS             <1..611
                     /locus_tag="PLY81_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28641.1"
                     /translation="AWIKNAEVYFNNNNELIINIEEKEPVARIFTLQESSFYVDTLAN
                     QLPISDKYSARVPVFTAFPTDHQQLSMADSLLMVDVIKLAKFIQADSFWNAQIAQVNI
                     LPNATFEIIPVLGNQIISLGNADELDKKFKRLLAFYKYAWLQNGINKYERLNVEFNNQ
                     VVAIKKGVSVFSDTTKAKQIIITVDSVKAETINSPKAVFKKN"
     gene            648..2036
                     /gene="ftsA"
                     /locus_tag="PLY81_00010"
     CDS             648..2036
                     /gene="ftsA"
                     /locus_tag="PLY81_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNF28642.1"
                     /translation="MNHNEAPIIVGLDIGTTKIAAIAGRKNEFGKLEILGFGKANSNG
                     VKHGQVLNIDQTIKSIQQALQNCLASNPDLEIKEVYVGIAGHHIKSLQTRGDIVRQQP
                     DDEIQGSEIDVLIDNQRKTFIPAGDQIIDVIPQEYYVDNIQNIKDPVGYNGVKVGANF
                     HIITGDKNAIKNINRAVNKSGLQTIDLVLQPLASSSAVMSDIDFEAGVVVLDIGGGTS
                     DLAIYYEGVLKHTAVIPFGGENITEDIRMGLGVLRQQAEALKVQFGSALVDDKKANSY
                     ITIPGIKGMPAKEIGVNNLSQIIMARMGEILDFVSYHLKQVGLDSKMLNGGIIITGGG
                     SQLKHLIQLTEYTTGLNARIGLPNEHLAAGHIEELKKPMYATCLGLILKGYNDHEHKY
                     KIFSESNPGISVPTSLKSDKEELEKNSKPEEPKSVDVEERAVKLKFWDKIGSKVKRNL
                     IELFKEEEDKTI"
     gene            2099..3943
                     /gene="ftsZ"
                     /locus_tag="PLY81_00015"
     CDS             2099..3943
                     /gene="ftsZ"
                     /locus_tag="PLY81_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNF28643.1"
                     /translation="MIHFDLPKQKSSIIKVLGVGGGGSNAVNFMFSQNVEGVDFIICN
                     TDAKAIEQSKVPNKIQLGPFLTQGLGAGANPEVGKAATEESLEEIKRILEVNTKMAFI
                     TVGMGGGTGTGGAPIIAKICKELGILTVGIVTTPFGFEGPRRMHQAEDGIKQLKPFVD
                     TLLVISNDKLRMQYGNLKMGEAFSKADNILSTAAKCITDIINSRGHIIVDFADVCTVM
                     KNGGVAILGKAEVEGENRAQLAIEEALNSPLLNDSDIKGAKWILININSAEGEHECTM
                     DELEIINNYLRMQAGEHTDVIVGTGYDNNLGGKIGITLVATGFDAKDPFAKPVEVKKE
                     MPKPEKIILTLDSDVEEKKEEVKPFIQQELPLVDKDPYAPRLLESQDELQNNNFQDVS
                     TSTPHNVELLSSVEEESPILHYELSTEITEEVNITIPSVIDEQTTSTTEENNEIETNR
                     FLTKPSNIYAEPDYEKPSSMSGHLMEPNLTTEQNEELPELEMKLVERDVNPVAADDAT
                     VKAQTEIPANLSAEEPVLLDDVEEQKRRAVERLQKLRNLSFNVNNTSDPNNEFDSVPA
                     YIRRNMELFGNTLTSVENFYSKYTVNKDENDQTQISTINTFLDGKKPD"
     gene            complement(4027..4662)
                     /locus_tag="PLY81_00020"
     CDS             complement(4027..4662)
                     /locus_tag="PLY81_00020"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF28644.1"
                     /translation="MSFTLIIIIVTAIISFSAFKNEKIVNDLIFYPPSITNQNQWYRF
                     ITCGFIHADVVHLAFNMLSLYMFGEAVENNFKYIFGTRGNVLYVVLYFSTLVICLLPT
                     YLKQKQNSYYRSLGASGAVSAIVFVGIFLFPTAKIGIFILPPIIPGFIFAPIYLYVSW
                     HLAKKGEDNINHSAHIWGAVYGLIFFIGACYILSSFDPVNNFIQSILEWAK"
     gene            complement(4710..5183)
                     /locus_tag="PLY81_00025"
     CDS             complement(4710..5183)
                     /locus_tag="PLY81_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNF28645.1"
                     /translation="MKIQIWSIGKQHESYIKEGVEDFTNRLTKYFTTEWKIIAPPKNN
                     AALNEKDLKKEEAGIILPLIQKADFLVLLDERGKQLSSIQLANFIELQANQSTKKIIF
                     LIGGAFGVDDTVFKRADTVLSLSQFVFPHMLVRLIIAEQLYRACTIIKNERYHHK"
     gene            complement(5199..7184)
                     /locus_tag="PLY81_00030"
     CDS             complement(5199..7184)
                     /locus_tag="PLY81_00030"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF28646.1"
                     /translation="MKRFKIILLLFVLVFNQNIYAQKKIALLIGIGKYPEGQRYWRNL
                     SSERDLQYMKAALLENGFTENNIVTIVNEKATKKAMVVALDKLADKAMAGDIVYFHFS
                     GHGQQIQDDAKDGKLDEADGYDEALIPYDAKGMWDEVDYKGQNHFRDDLLEEKLIAIR
                     RKVGSKGSVVVVLDACHSGTATRSASIVRGVPEPCKSNNYTPNINLNLSITETGFLSS
                     IKEKLGSLVVFSGSSPNQANKETTDKNGKSVGSLSYAFSKAITNLPINCNYNLLFEKV
                     KATVQADEPTQIPMFEGNGTLKVFSNQFIPLKDIIAVEQRFNNAANNFNDTTFVIQRG
                     QFNNINTGTELNVYVMGTTELYCKAFVQQANNFQSICIATKKLSNKNTYEVKVESLVY
                     GAVTASYNIQNKIPTATNAIEKQLQHFLQQQPFLLKNNNPDYTLNISQENNSTYIQLI
                     EKNDSVRYSSKFSTNDSLSANNLEDLLESIKKGMRVKYLRKMEDGGSLYNNVQVTIHP
                     LQKAVSDNELIFYPNDSFQLKIKSNYDGILYFTVLDLLPDNNIKILIPDTSKLANDAD
                     YSLRKNEERQFTIYTDETSITGKEFLKVIFSPYPIDLRPSFQLYSTRTINTKLKPIEK
                     AMDDLFPKNKNTLTRSTPVKMDDIAIKTVGFTLRKSK"
     gene            7365..7436
                     /locus_tag="PLY81_00035"
     tRNA            7365..7436
                     /locus_tag="PLY81_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7398..7400,aa:Arg,seq:ccg)
     gene            7533..10019
                     /locus_tag="PLY81_00040"
     CDS             7533..10019
                     /locus_tag="PLY81_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNF28647.1"
                     /translation="MTNKRLITLLAFIFIAAVVYAQPKANYRLASKYSPKKLDKIVYS
                     LNVDPHWLKNGNRFWYVYETNQGKKWYIVDANKGEKKYLFDNDKMAAEISKIVKDPFD
                     AQNLGLDSIRFIGDETKFQFEVKSTEEIEKKDTTAKDKKDAKPIKEKKVFYFEYDINT
                     GLVTELQGFKKPKRKPFWANMSPDGNTIVFGKHHNLFYMDKANYEKALLNEKDSTIVE
                     HPLTTDGEEYFSYYGGGAYSGNGENNVEKEKNKDDRKPVFIMWSPDSKHFALTKSDAR
                     KVKELWVINSAADPRPTIETYKYWMPGEKESPKDYMMVFNMNTKKGKEVKVSNFKDQD
                     LNLYSQPSLQNTRDDMFKPFLWLGTNTQFYFSRTSRDLKKIDQCLYDISADSVKVLIE
                     ERMNTSIEIRRPGLVNNGKELIEWSERDGWAHFYLYDENGKLKNQITSGPWHCENIIN
                     IDEKKRVLYFTANAKEKNENPYYLHLYKVNFDGTGLKLLNEGDFDHQISLNDNNAFFV
                     NNFSRVNTTPKSVLYNNEGKKVLDLETADLSSLFATGYKFPEPFKVKADDGITDLYGV
                     MYKPFDFDSTKKYPLIEYVYPGPQTEAVNASFSRGMDRVDRLAQLGFIVVTVGNRGGH
                     PSRSKWYHNFGYGNLRDYGLADKKATAEQLADRFSFIDINKVGIHGHSGGGFMSTAAM
                     LVYPDFFKVAVSNAGNHDNSVYNRWWSEKHHGVKEIISAKGDTSFAYSIDKNPDVAKN
                     LKGRLLLIHGEIDNNVHPANTMRVVNALIKANKRFDMLLLPTQRHAFGNMTEYWFWKT
                     ADYFTYWLMGDKTDHEVDIEEINREIEKKK"
     gene            10155..10961
                     /locus_tag="PLY81_00045"
     CDS             10155..10961
                     /locus_tag="PLY81_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF28648.1"
                     /translation="MENFTQKVIVITGGSDGIGKALVEAFLKLGAKVATCGRNYDKLY
                     QLQSSHTGMPLLIHTADVSNEQECINFIHTVVKNFGTIDILINNAGVSMRSLFEDVEI
                     EALRKVVDVNLWGTVNCTKYALPYILKSKGSIVGISSIAGFRGLPGRTGYSASKFAVN
                     GFLEALRTELLHSGVNVMWICPGFTASNIRRAALNYKGQAHGETSMDEGKMMSAEDCA
                     THIIKAIYKRKRTLILTNTGKKTVWLNKFLPALADKFTYNYFFKNGKLFK"
     gene            10991..11812
                     /locus_tag="PLY81_00050"
     CDS             10991..11812
                     /locus_tag="PLY81_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNF28649.1"
                     /translation="MPLITLATDIGQQDYIVGAFKGQLHAEISQLSIADISHYLPLDN
                     FAHAAYICSNAYKYYPSNTLHILLINVFDEPETHFVICNYNNQFILCPNNGILSMLVK
                     TLPENTTVLPIINATNIMQITKIIATTASTILANNTIEGIGTKGLKIVEKAMMKPTVG
                     NDWIDGHILFIDNFNNVIVNITEEVFENARKGRNYKIVFKRNEVVKNLSTNYASVKET
                     EQLAWFNAAGHLELAINKGFAAELFGLESYKEKMHQEGKSVQNKWFYQTVRIYFE"
     gene            11883..12008
                     /locus_tag="PLY81_00055"
     CDS             11883..12008
                     /locus_tag="PLY81_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28650.1"
                     /translation="MTTKKNSVAFHTLGCKLNFSETSTLSRMMEKEGFEKKLLKM"
     gene            12023..13177
                     /gene="mtaB"
                     /locus_tag="PLY81_00060"
     CDS             12023..13177
                     /gene="mtaB"
                     /locus_tag="PLY81_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNF28651.1"
                     /translation="MNTCSVTDNADKECRQIVRRIHKQAPESVVVVTGCYAQLKPKEI
                     AEIPGVDLVLGAAEKFNLAQHISEFTKGDSTKICSCDTSDIAGFNASFSANDRTRTFL
                     KVQDGCDYNCSFCTIPMARGKSRSDSISNVLNSAKELASKGVKEIVLTGVNLGDFGKG
                     SQGAGVSISMQEREEDFFDLIQALDAIDGIERFRISSIEPNLLTNPIISFVAKSKKFM
                     PHFHIPLQSGSNHILKLMRRRYQRELYQDRVAYIKSLMPHCSIGVDVIVGFPGETDTY
                     FEETFNFLHNLDVSYLHVFTYSERDNTVALNLKPVIPVNIRHQRNKTLRNLSFMKLQY
                     FTQQHSGQNRKVLFESADKNGMMEGYTDNYIRISTPFRKEWENQIIDWKI"
     gene            13177..14262
                     /gene="recF"
                     /locus_tag="PLY81_00065"
     CDS             13177..14262
                     /gene="recF"
                     /locus_tag="PLY81_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626633.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNF28652.1"
                     /translation="MLQLNNIALLQFKNYTQQQFVFTQKVVGICGRNGSGKTNLLDAI
                     YYLCFTKSYFLASDAQNVQIGKAGMRIEGNMLLNNTTQNLICILRENNKKEFLYNKEQ
                     LKKFSEHIGRFPCVIISPDDTTLVTGSSELRRKYIDTIIAQINSHYLYNLVDYNKILV
                     QRNSLLKQAAEVGTINKDVVEILNQQLATKANYIFEERKLFFKQYIPFVYDYYSKIAS
                     KTDNITLHHQSALYTETIENLLEQNLQKDIALQRTTAGIHRDDIEINMNGALFKTIAS
                     QGQRKSLLFALKLAEWQIIKQKKLYSPILLLDDVFEKLDGERMHNLLQWVCNEESTQV
                     FITDTHKERLEQALQNAAINFQLIELQ"
     gene            complement(14276..14794)
                     /locus_tag="PLY81_00070"
     CDS             complement(14276..14794)
                     /locus_tag="PLY81_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28653.1"
                     /translation="MKSVKYYIHFVWMLLFSVVAVNAQNSFNPTGYATSLKEALKEPE
                     NISTISVYYTRGMVFDIEQGKAAPDASFEKLMELNNLRTFRLNGCPLDFNQELFFCNL
                     SKVKQLEALELRMNFRQLGLLTEQSIKCLKKLKNLRRLNLPCQYPQEDYIKLQQQLPD
                     CEIIINVYPGGE"
     gene            15029..15823
                     /locus_tag="PLY81_00075"
     CDS             15029..15823
                     /locus_tag="PLY81_00075"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNF28654.1"
                     /translation="MQQYQQLLQHIINTGERKEDRTGTGTISCFGYQMRFDLQKGFPL
                     VTTKKLHLKSIIYELLWFLNGDTNIKYLNNNGVSIWNEWANDNGDLGPVYGKQWRSWQ
                     GANGKTIDQIADVIKQLKTNPDSRRMIVSAWNVAELSEMALMPCHALFQFYVANGKLS
                     CQLYQRSADVFLGVPFNIASYAALTMMIAQVCNLQLGEFIHSFGDVHLYCNHIEQAQL
                     QLTRTPFELPILKINSSVKDIFNFQYEDFILENYQFHPHIKAVVAV"
     gene            15867..16361
                     /locus_tag="PLY81_00080"
     CDS             15867..16361
                     /locus_tag="PLY81_00080"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNF28655.1"
                     /translation="MIISLIVAASENNVIGKNNQLLWHLPNDLKFFKNTTWGMPVVMG
                     RKTFESLGNKPLNGRLNIVITNQKKWNANGVVVVNSLDDAIFVAKSHSYKEMFIAGGG
                     LIYNLVMNKADKIYLTRVHTAIEGDTFFPEINEKQWQKVQDNFCNADEKHAFNYSFQL
                     WERK"
     gene            16464..17543
                     /locus_tag="PLY81_00085"
     CDS             16464..17543
                     /locus_tag="PLY81_00085"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNF28656.1"
                     /translation="MSITQIPKFPVVKDSLHAELRKRVQQYFDDKGIKATGNISLFTK
                     AIILVTAFVLVYIHLIFFTPHWYIAIPECIILGCLIAAIGFNVMHDGSHGSFSKHKWK
                     NKLAAASLSMLGANHFMWNMKHNMLHHSFTNVDGVDDDIEIGVLMRMAPTQKRYKLHR
                     FQHFYFWFLYMLLYVFWIFFTDYHKYFTQKIGNVPLKKMATKDHIEFWLTKIYHGFIF
                     IALPIIMVGWFYWLMGFLVATLGSGFVLSIVFQLAHTVEHTHFPIADFDNNKLPDEFA
                     AHQIKTTANFATKNKIVSWLVGGLNFQIEHHLFPKISHVHYPAISKIVKSVCAEYQLT
                     YIEYPTVSRAIVAHVRFLKHLGKYD"
     gene            17567..18070
                     /locus_tag="PLY81_00090"
     CDS             17567..18070
                     /locus_tag="PLY81_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28657.1"
                     /translation="MTKKIILAGIATVVMIVIMRLLSINLVTDYSPNGIVSFELAKSY
                     RDASAIMMAVGVKPLQINVAVDFAFIIAYCFFLYLCCKALLNQYTKQVLKTIGFIFLE
                     LSIFIGILDLVENIAMLITLGGYGTKISVSVSKWAAIAKFSIAGLVIVYILIASILLY
                     FSSRKKA"
     gene            18070..18810
                     /gene="rlmB"
                     /locus_tag="PLY81_00095"
     CDS             18070..18810
                     /gene="rlmB"
                     /locus_tag="PLY81_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNF28658.1"
                     /translation="MFIPKKNIIVGRNPVVEALKQHLTIDKILVSKKASGEIVNEIKQ
                     LARANNVPVQYVPIEKINSLTNIQHQGIVAFKSAVRYFNLQHVIDFVVAKGETPLFLI
                     LDGVTDVRNIGAIARTAVCCGAQAIIIPDKGVGALNEDAVKTSAGALEQIHICRVNSL
                     MIAVDELRLNGISVFASEMTATTKLYNLNLNIPCAIVMGSEEKGVYPALMKICENTFH
                     IPMANNFESLNVSVATGMILYEAMKQRK"
     gene            complement(18839..19189)
                     /locus_tag="PLY81_00100"
     CDS             complement(18839..19189)
                     /locus_tag="PLY81_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNF28659.1"
                     /translation="MSNKKSKADKDGFVYSTNPNFNFNNEEETVKTLLPNLQKLRIWL
                     DTKQRAGKTVTLITGFVGTLADLEKLAKQLKNFCGTGGNAKNNEIIIQGDQREKILQW
                     LIKNGYSNSKKAGN"
     gene            complement(19289..19450)
                     /locus_tag="PLY81_00105"
     CDS             complement(19289..19450)
                     /locus_tag="PLY81_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28660.1"
                     /translation="MEKTKSKSKYYLLFIFWLAIIVFLLATPDFRQWFWLALPGTCTY
                     LAYSLDLID"
     gene            complement(19487..19921)
                     /locus_tag="PLY81_00110"
     CDS             complement(19487..19921)
                     /locus_tag="PLY81_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF28661.1"
                     /translation="MTVNTIIILIIIGVAAGMLSGLVGVGGGIIIVPALIYILGFSQK
                     TAQGTSLGLLLLPVFIVSLIYYYKAGEVNIKAIPFLAIGFLVGGYLGSKLALSLPDAT
                     VKKIFAFLMIIVAVKMLFFDKPKKDTNKIHATEAQKNKSVGS"
     gene            complement(19929..20477)
                     /locus_tag="PLY81_00115"
     CDS             complement(19929..20477)
                     /locus_tag="PLY81_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNF28662.1"
                     /translation="MQQLVQHPWHGVNPGEQAPRKVNAVIEIPQGSRCKYEIDKPSGL
                     LKLDRIIYSSFYYPCNYGFIPQTYGGDKDPLDILVITSIPVVPLTLMEAKVVGVMQMI
                     DSGDADDKIIAVASNDPGVNHYNNIEELPKHFFDELRHFFEEYKKLENKEVKVEEFGD
                     KTTALKIVSEAIEFYKKSFLNK"
     gene            complement(20496..20858)
                     /gene="crcB"
                     /locus_tag="PLY81_00120"
     CDS             complement(20496..20858)
                     /gene="crcB"
                     /locus_tag="PLY81_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNF28663.1"
                     /translation="MLKNILLVGIGGGLGSIFRYFIGLMITNKHFPYATITVNVVGSF
                     FIGVIMAILAKQTHNVEHWYLIITTGFLGGFTTFSAFSWNTFQLMQQQQYAASFLYIG
                     LTFFLTLIAVSIGFFIFK"
     gene            complement(20858..21259)
                     /locus_tag="PLY81_00125"
     CDS             complement(20858..21259)
                     /locus_tag="PLY81_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28664.1"
                     /translation="MKIFFSLIAFLLLACNNSSITYLPATRPLEAAREFIEAFKAGKF
                     EKASFYMLHDKENDKLLKEAKDKYYQFTKKDKRLLSEASIQNVTIETISTTETIIYYN
                     NSFDKERRKVKAVNQNNTWLIDFKYTFNPNL"
     gene            complement(21393..22388)
                     /locus_tag="PLY81_00130"
     CDS             complement(21393..22388)
                     /locus_tag="PLY81_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF28665.1"
                     /translation="MTDTIINLDSINPIEFFGVNNGKFDLLKKKFPLLKILSRGTHIK
                     LSGAPEQIDSAKEKINLIVQYLERNGHLSENYFEQILGGDDAETIDNFVDKNPNDILV
                     FGPNGKTVRARTANQKRMVTAADKNDIVFAIGPAGTGKTYTAVALAVRALKNKVVKKI
                     ILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMTTRTIEIAPL
                     AYMRGRTLDNAFIILDESQNTNDLQLKMFLTRIGANAKAIITGDPTQIDLPRNQRSGL
                     EKAVRILKNIDGIAHIELDEEDVVRHKLVKAIIKAYDKEFEKEEAEYEKKYNHPR"
     gene            complement(22563..>23175)
                     /locus_tag="PLY81_00135"
     CDS             complement(22563..>23175)
                     /locus_tag="PLY81_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF28666.1"
                     /translation="NMLINTTSLKFEDQLTTSGFTGVTSEIFNNPSSLKSNFIDLNAG
                     VLYTGSSSDKNSYYAGVSIYHVTRPKQTFTGANYLLNMRTTIHGGGNFDMNDNTTLHV
                     SALQSFQGGAAETVVGGAIQFNVSGNEAPKPTSFYAGAWLRFKDALIPYIGLEFDDFR
                     LGATYDINTSSLKNASQKRGGLEISLIYIRRPNTDRNIRCPKF"
BASE COUNT         8315 a         3329 c         3779 g         7752 t
ORIGIN      
        1 atgcttggat aaaaaatgcg gaagtttatt ttaacaacaa taatgagtta attataaaca
       61 tagaggagaa agaaccagta gcaagaattt ttaccttaca agaaagttct ttttatgttg
      121 atacactagc aaaccagttg ccaattagcg ataaatatag tgctagagtt cctgtattta
      181 cggcattccc aacagatcat caacaacttt ccatggcaga tagcttgtta atggtagatg
      241 taataaagtt ggcaaaattt atacaggcag atagtttttg gaatgctcaa attgcacaag
      301 taaatatttt acccaatgca acatttgaaa ttattcctgt gttgggtaac caaataataa
      361 gtttaggtaa tgcagatgaa ttggataaaa aatttaaaag actgctggct ttttataaat
      421 atgcttggtt gcaaaatgga ataaacaagt atgagagatt aaatgttgaa tttaataatc
      481 aagtagtagc tattaaaaaa ggggttagtg ttttttcaga tactacaaaa gccaaacaaa
      541 taataataac agtagattca gtaaaggcag aaactattaa ttcacctaaa gcagtattca
      601 aaaaaaatta aaaattaata ttataaacaa caacaaaaca catatctatg aatcacaatg
      661 aagcacccat tattgttggg ctcgatattg gaactacaaa aattgcagca attgctggta
      721 gaaaaaatga gtttggaaaa cttgaaatct taggctttgg caaggccaat agtaatggcg
      781 taaaacatgg ccaggtttta aatatagatc aaaccataaa gtctatacaa caagcattgc
      841 aaaattgttt agctagtaat ccagatttag aaattaaaga agtatatgtt ggcatagctg
      901 gccatcatat taaaagttta caaacccgtg gagatatagt aagacagcaa ccagatgatg
      961 aaattcaagg atctgaaatt gatgtgctga tagacaatca acgtaaaaca tttattcctg
     1021 caggagatca aattattgat gttattcctc aagaatatta tgtagataat attcaaaata
     1081 ttaaagatcc tgttgggtat aatggtgtaa aagtgggtgc aaattttcat atcataacag
     1141 gggataaaaa tgcaattaaa aatattaata gggctgtaaa taaaagtggt ttgcaaacta
     1201 tagatcttgt attgcaacct cttgcaagtt ctagtgctgt aatgagtgat attgattttg
     1261 aggcaggtgt tgtagtgcta gatattggtg gaggaacaag cgatttggca atttattatg
     1321 aaggtgtttt aaaacatact gcagtcattc cttttggagg cgaaaatatt acagaagata
     1381 taagaatggg gttaggcgtt ttacgtcagc aggcagaagc tttaaaagtt caatttggta
     1441 gtgcattagt agatgataaa aaagctaatt cctatataac aataccagga ataaaaggga
     1501 tgccagctaa agaaattggg gtaaataatt tatctcaaat tattatggca agaatgggag
     1561 aaattttaga ttttgttagc tatcatttaa aacaagtagg cttagatagc aaaatgctaa
     1621 atggaggaat aataattact ggtggaggct ctcaattaaa acacctaata caactaacag
     1681 aatataccac aggtttaaat gcaagaattg ggttaccaaa tgagcatctt gctgcaggcc
     1741 atatagaaga acttaaaaag ccaatgtatg caacttgttt gggtttaata ttaaaaggct
     1801 ataacgatca cgaacacaaa tacaaaatat tttcagaaag taatcctggt atttctgtac
     1861 ctactagttt aaaatcagat aaagaggaac tagaaaaaaa tagtaaacca gaagaaccca
     1921 aatcagtaga tgtggaagaa cgtgcagtta aattaaagtt ctgggataag ataggcagca
     1981 aagttaaaag aaacctaatt gagctgttta aagaagaaga ggataaaact atttaatatt
     2041 aaattctaac aattactaac caataaaaat aaaaattaac ccattaaaat taataactat
     2101 gatacacttt gatttgccaa aacaaaaatc atctattatt aaagttttag gtgtaggtgg
     2161 cggaggaagc aatgctgtaa actttatgtt ttctcaaaat gtagaaggcg tagattttat
     2221 tatttgcaat accgatgcta aagcaattga acaaagtaaa gttcctaata aaattcaatt
     2281 aggcccattt ttaacacaag gtttgggtgc tggtgccaat ccagaagttg gtaaagcagc
     2341 aacagaagaa agcttagaag aaataaaacg cattttagaa gtaaatacca aaatggcttt
     2401 tattactgtt ggtatgggtg gaggaacagg aactggtggt gcaccaatta ttgcaaaaat
     2461 ttgtaaagaa ctaggtattt taacagtagg aatagtaact acaccttttg gttttgaagg
     2521 tccaagacgt atgcaccaag cagaagatgg tattaaacaa ttgaaaccct ttgttgatac
     2581 tttattagta attagcaacg ataaattaag aatgcaatat ggtaatttaa aaatgggaga
     2641 agcattttct aaagcagata atattttatc tactgctgca aaatgtatca cagatattat
     2701 caatagccgt ggacatatta ttgttgactt tgcagatgtt tgtactgtaa tgaaaaatgg
     2761 tggagtagct attttaggta aggcagaagt agaaggagaa aacagagccc aacttgcaat
     2821 tgaagaagca ctcaactctc cattattgaa tgatagtgat ataaaaggag ctaaatggat
     2881 attaataaat ataaatagtg ccgaaggtga acatgagtgt acaatggatg aattagagat
     2941 tataaataat tacttgcgta tgcaagcagg agaacataca gatgttattg ttggtacagg
     3001 atatgataat aatttagggg gtaaaattgg tataacatta gtggctactg gttttgatgc
     3061 aaaagaccca tttgcaaaac ctgtagaagt aaaaaaagaa atgccaaaac ctgaaaaaat
     3121 tatcttaaca ttagatagcg atgttgaaga gaaaaaagaa gaagtgaaac ctttcattca
     3181 acaagagttg ccattagtag ataaagatcc ttatgcacca agattattag aatcacaaga
     3241 tgaattacaa aacaataatt ttcaggatgt ttccacaagt actccacaca acgtagaatt
     3301 acttagttca gtagaagaag aaagccctat tttgcactat gagttaagta ctgaaataac
     3361 agaggaagta aatataacta taccttcagt tatagatgaa caaactacat caactactga
     3421 agaaaataat gaaattgaaa ccaataggtt tcttacaaaa ccatctaata tctatgcaga
     3481 gcctgactat gagaagccat cgtctatgtc cggacatctt atggaaccta atcttactac
     3541 tgaacaaaat gaagagctac cggagctcga aatgaagttg gtagaaagag atgtaaatcc
     3601 agtggcggcg gatgatgcaa cagttaaagc tcaaacggaa atacctgcta atttgtctgc
     3661 agaggaacca gttttactgg acgatgtaga agagcaaaaa cgcagggcgg tagaaaggct
     3721 acagaaatta cgtaatctgt ctttcaatgt taacaatacc tcagatccta acaatgagtt
     3781 tgattctgtt cctgcttaca ttcgccgcaa catggaatta tttggaaata cgcttacatc
     3841 ggttgaaaac ttttacagca agtacactgt aaataaagat gaaaatgatc aaactcaaat
     3901 ttctactata aatacttttt tagatggaaa aaaaccagat taaataggtt ggcttttaga
     3961 gattttcttt tgaatgtgtt ttttgttgtg ataatcgctt ctgcaaagaa gcgattattt
     4021 ttttaactac tttgcccact caagtataga ctgaataaaa ttatttacag gatcaaaact
     4081 gcttaaaata taacaagctc ctataaaaaa tattaatcca tatacagcac cccaaatatg
     4141 agcagagtgg ttaatattat cttcgccctt tttggctaaa tgccaggaaa catataaata
     4201 tattggtgca aaaatgaacc ctggaataat tggaggtaaa ataaatattc caatttttgc
     4261 agttggaaat aaaaaaatac caacaaaaac aatggcagat acagcaccag atgctcctaa
     4321 acttctataa taactatttt gtttttgttt taaataagtt ggcaataaac atattactaa
     4381 tgtactaaaa tataacacta cataaagaac atttcctcta gtaccaaaaa tatatttaaa
     4441 attattctca accgcctctc caaacatata taacgataac atattaaaag caagatgtac
     4501 aacatctgca tgaataaagc cacaagtaat aaatctatac cattgatttt ggttggtaat
     4561 ggagggtgga taaaaaatta aatcgttaac tattttttcg tttttaaatg cagagaatga
     4621 aataatagct gttactataa ttataattaa ggtaaaactc attatacaaa attaattaat
     4681 tgagaatcta ataaaataaa taaaaaaaat tatttgtgat gatacctttc gtttttaata
     4741 atagtacaag ccctatacaa ctgctctgca ataattaatc ttactagcat gtgaggaaaa
     4801 acaaattgag ataaacttaa aacggtatca gctcttttaa aaactgtatc gtcaacgcca
     4861 aatgcaccac caattaagaa tatgattttt tttgtacttt ggtttgcttg cagctctata
     4921 aagttagcta attgaataga ggataattgt ttgccccttt cgtctaataa aaccaaaaaa
     4981 tctgcttttt gtattaaggg taaaattatt ccagcttctt ctttttttag atccttttca
     5041 tttaatgctg cattgttttt tggtggggca ataattttcc attctgttgt aaaatattta
     5101 gtaagcctgt tagtgaaatc ttcaacaccc tcttttatat aactttcatg ctgcttgcct
     5161 atgctccaaa tttgaatttt cattttttgg aaataatttt atttgctttt tcttaaagta
     5221 aaaccaactg ttttaattgc aatatcatcc attttaactg gggtacttct ggttaatgta
     5281 tttttatttt tcggaaataa atcatccata gctttttcta ttggttttag ttttgtgttt
     5341 attgttcttg ttgaatacag ttgaaaagat ggccttaaat caataggata gggagagaaa
     5401 ataactttta aaaattcttt accagtaata ctcgtttcat cggtatatat agtaaactgc
     5461 ctttcttcat tttttcttaa agagtaatct gcatcattag caagtttaga agtatcgggt
     5521 attaaaattt ttatattatt atctggcaat aaatctaata cagtaaaata taaaatacca
     5581 tcgtaattgc ttttaatttt taattgaaaa ctatcatttg gataaaaaat taattcatta
     5641 tcgctaacgg ctttttgcaa tggatgtatg gttacttgta cattattata aaggcttcca
     5701 ccatcttcca tttttcgtaa atattttact ctcatgcctt tttttatact ttctaacaag
     5761 tcttctaaat tgtttgcact taaggaatca tttgtagaaa attttgaaga gtacctaaca
     5821 ctatcattct tctcaattaa ttgaatgtag gttgaattgt tttcctgaga aatgtttaag
     5881 gtataatctg gattattatt ttttaataaa aaaggttgct gttgcaaaaa atgttgcaat
     5941 tgtttttcta ttgcatttgt agcagttggt attttatttt gaatattgta agatgctgta
     6001 acagcaccat atactagaga ttcaactttt acttcgtaag tatttttatt agataatttt
     6061 ttagtagcaa tacatatact ttgaaaatta ttggcttgtt gtacaaatgc tttacaatac
     6121 aattctgtag tgcccataac ataaacattt agctctgttc cggtattaat gttattgaat
     6181 tggcctcttt gaataacaaa agtagtatcg ttaaaattat ttgctgcatt gttaaaacgt
     6241 tgctccactg ctattatatc ttttaaagga atgaattgat tactgaatac ttttaaggtt
     6301 ccgttacctt caaacatggg tatttgagtt ggctcatctg cttgtactgt tgcttttact
     6361 ttttcaaata ataaattata attacagtta attggcaagt tagtaatagc tttagaaaat
     6421 gcataagaca aagacccaac actttttcca tttttgtcgg ttgtttcttt atttgcctgg
     6481 ttagggctac ttccactaaa tactaccagg cttcctaatt tttctttaat actacttaaa
     6541 aacccagttt ctgttataga taaattaagg tttatattgg gtgtgtaatt attactttta
     6601 caaggctcag gtacaccacg tacaatgctt gcacttcttg ttgcagtgcc agaatggcaa
     6661 gcatctaaaa caactacaac acttccttta ctacctactt ttcttcgaat ggcaatgagt
     6721 ttttcttcta acaaatcatc tctaaaatgg ttttggcctt tatagtccac ctcatcccac
     6781 atgccttttg catcataagg tatcaatgct tcatcgtatc catctgcttc atctaattta
     6841 ccatcttttg catcatcttg tatttgttga ccatgccccg aaaaatgaaa ataaacaata
     6901 tcgccagcca ttgctttatc tgctaattta tctaatgcaa ctaccattgc ttttttagtt
     6961 gctttttcat ttacaattgt tacaatatta ttttcggtaa agccattttc taataaagct
     7021 gctttcatat attgtaaatc tctttcagag cttaagtttc tccagtagcg ttgtccttct
     7081 ggatattttc caattccaat aagcaatgct attttttttt gagcatatat attttggtta
     7141 aaaactaata caaatagcaa cagtataatt ttaaaccttt tcataatttt attttattca
     7201 aatttaatac attatgtaaa acattgctgt aagtgacatt tttagattac tgttttatgt
     7261 taatttttaa atgctattta aaaaattaat tattatagaa atctatttat tgaaagctca
     7321 aaaaattaaa cctgaaccat aaatctttac ttttgttgtt taatggcctt gtagtacaat
     7381 ggatagtaca gaagtttccg aagcttctga ttcaggttcg attcctgacg aggccactta
     7441 tagttttttt aaaaataagt tcagtttttt tataaatggt aattaattac tgttttttgt
     7501 tgttattttt ccgctttcta aaacttcaaa ccatgactaa caaacgcctt attacactac
     7561 ttgcatttat ttttattgca gcagtggtgt acgcacaacc aaaagctaat tatagattag
     7621 cttctaagta ttctcctaaa aaattagata aaattgttta ttccttaaat gttgatccac
     7681 attggctaaa aaatggaaac cgtttttggt atgtttacga aaccaaccaa ggtaaaaaat
     7741 ggtatattgt tgatgctaat aaaggagaaa aaaagtatct ttttgataac gataaaatgg
     7801 ctgctgaaat ttctaaaatt gtaaaagacc cattcgatgc tcaaaatctt ggattagaca
     7861 gcattcgctt tattggcgat gaaactaaat ttcaatttga agtaaaaagt acagaagaaa
     7921 tagagaaaaa agataccaca gcaaaagata aaaaagatgc taagcccata aaagaaaaaa
     7981 aggttttcta ttttgaatat gatattaata caggtttagt aacggaattg caagggttta
     8041 aaaaaccaaa acgcaaacca ttttgggcta atatgtcgcc agatggcaat acaattgttt
     8101 ttggcaaaca tcataatttg ttttatatgg ataaagccaa ttatgaaaaa gcattattaa
     8161 atgaaaaaga tagcacaatt gtagaacacc ctttaactac agatggagaa gaatatttct
     8221 cgtattatgg tggaggtgca tacagtggta atggggaaaa taatgtagaa aaagaaaaaa
     8281 ataaagatga tcgcaaacca gtttttatca tgtggagccc agatagtaaa cattttgcac
     8341 ttaccaaatc tgatgctaga aaagtgaaag aattatgggt aattaacagt gctgcagatc
     8401 caagaccaac aattgaaact tacaagtatt ggatgcctgg agaaaaagaa agtcctaaag
     8461 attatatgat ggtgtttaat atgaacacta aaaaaggaaa agaagttaaa gtaagcaatt
     8521 ttaaagatca ggatttgaat ttgtatagcc aaccatcttt gcaaaataca agagacgata
     8581 tgtttaaacc atttttatgg ttaggaacta atacacaatt ttattttagc agaacaagta
     8641 gagaccttaa aaaaattgac caatgtttat acgacatctc tgccgattcg gttaaagttc
     8701 ttattgaaga aagaatgaac acctccattg aaataagaag accaggactt gttaataatg
     8761 gtaaagaatt aattgagtgg agcgaaagag atggctgggc tcatttttat ttgtacgatg
     8821 aaaatggaaa attaaaaaac caaatcacga gtggcccatg gcattgtgaa aatataatca
     8881 atattgatga gaaaaaaaga gtgctgtatt ttactgctaa tgcaaaagag aaaaatgaaa
     8941 acccttacta ccttcattta tacaaagtaa attttgatgg tactggttta aaattattaa
     9001 acgaaggaga ttttgatcac caaattagcc tgaatgataa taacgctttt tttgtcaata
     9061 atttttcgag agtaaacact actccaaaat ctgttttata taacaacgaa ggcaaaaaag
     9121 tattagattt agaaacagca gatttaagct cgttatttgc tacagggtat aaatttccag
     9181 aaccatttaa agttaaagca gatgatggta ttacagactt atatggtgta atgtataagc
     9241 cttttgattt tgatagcaca aagaaatatc cattgattga atatgtttac cctggcccac
     9301 aaacagaagc tgtaaatgca agttttagta gaggaatgga tagagtggat agactagcac
     9361 aacttggttt tattgtagtt actgttggta atagaggagg gcatccatcc agaagtaaat
     9421 ggtaccacaa ctttggttat ggaaatttaa gagattatgg cttagccgat aaaaaagcaa
     9481 ctgctgaaca attagcagat agattttctt ttattgacat aaacaaagta ggtattcatg
     9541 gccatagtgg tggtggtttt atgagtactg ctgccatgtt ggtttatcca gatttcttta
     9601 aagttgctgt aagcaatgca ggtaatcacg ataacagtgt ttacaatcgt tggtggagtg
     9661 aaaaacatca tggagtaaaa gaaattatta gtgctaaagg agatacaagc tttgcatata
     9721 gtattgataa aaacccagat gttgctaaaa acttaaaagg aagattatta ttaattcatg
     9781 gagaaattga taacaacgta catccagcaa acacaatgag ggttgtaaat gctttaataa
     9841 aagctaataa aagatttgat atgttattat taccaacaca acgacatgct tttggtaata
     9901 tgacagaata ttggttttgg aaaactgcag attacttcac ctattggcta atgggagaca
     9961 aaactgacca tgaagtagat attgaagaaa taaacagaga aattgaaaag aagaaataac
    10021 ctaaccaggc tatttctttc taaaaggttg catagtttta atgcaacctt ttttaattaa
    10081 aatttattca aactttaaaa taaaactcat acctaacagc ttatttttgc aataattatt
    10141 tataaagatt ttgcatggaa aattttaccc aaaaagtaat tgtaataaca ggcggttctg
    10201 atggaattgg aaaagcttta gtggaagcat ttttaaaatt aggagccaaa gtggctacat
    10261 gtggaagaaa ttatgataaa ctgtatcagt tgcaatcttc acacacagga atgcctcttt
    10321 taatacatac tgcagatgta agtaacgaac aagaatgtat aaactttatt catactgttg
    10381 taaaaaattt tggaactatt gatattttga ttaacaatgc tggagtttct atgaggtcat
    10441 tatttgaaga cgtagaaata gaggctttac gaaaagttgt agatgttaat ttatggggta
    10501 ctgtaaattg taccaaatat gcactgccat acattttaaa atcaaaaggc tctatagttg
    10561 gtatatcttc tattgcaggc tttagaggct tgccagggcg tactggttac tcagcatcta
    10621 aatttgcagt taatggtttc ttagaggcat taagaacaga acttttacac tctggtgtta
    10681 atgtaatgtg gatttgccca ggctttacag cttccaatat tagaagggca gcattaaact
    10741 ataaagggca ggctcatggc gaaacatcta tggatgaagg taaaatgatg agtgccgaag
    10801 attgtgcaac gcatataatt aaagcaatct ataaaagaaa acgaacttta attttaacaa
    10861 acacaggtaa aaaaactgtg tggttaaata aatttttacc agctttagca gataagttta
    10921 cctataatta ttttttcaaa aacggaaaat tattcaaata accaattcaa ctttatttct
    10981 atcttaaaat atgcctttaa taacacttgc aacagatatt ggtcaacaag attatattgt
    11041 tggtgctttt aaagggcaat tacatgcaga aatttcacaa ctatctattg cagatatttc
    11101 acactatttg ccattagata actttgcaca tgctgcgtat atatgtagca atgcttataa
    11161 atattatcct tcaaacacac tgcatatttt actaataaat gtttttgatg aacccgaaac
    11221 tcattttgtt atttgcaatt ataataatca atttattctt tgtcctaaca atggcatcct
    11281 aagtatgctg gttaaaacat tgccagaaaa tacaactgta ttgcctatta ttaatgcaac
    11341 taatattatg caaattacta aaattattgc tactactgca agtaccattc ttgcaaataa
    11401 tactattgaa ggcattggaa caaaaggatt gaagattgta gaaaaagcaa tgatgaaacc
    11461 cactgttggc aacgattgga tagatggcca tattttgttt attgataact ttaataatgt
    11521 aattgtaaat attacagaag aggtttttga aaatgcaaga aaaggaagaa actataaaat
    11581 agtttttaaa agaaatgaag tagttaaaaa cttaagtacc aactatgcat ctgtaaaaga
    11641 aacagaacaa ttagcttggt ttaatgctgc aggtcattta gaattagcta ttaacaaagg
    11701 ctttgctgca gagttgtttg ggctagaaag ctataaagaa aaaatgcatc aagagggtaa
    11761 aagcgttcaa aataaatggt tctatcaaac agttcgtatt tactttgaat aagtataatt
    11821 tattgtaaag cctataatac ggccctccaa aaattcacct tttaattaat tacctttgca
    11881 aaatgacgac aaagaaaaat agtgtagctt ttcatacttt aggctgtaag ttaaattttt
    11941 ccgaaacctc tacattatca agaatgatgg agaaagaagg attcgaaaaa aaacttttga
    12001 agatgtagca gatatttatg ttataaacac ttgctctgta accgataatg cagataaaga
    12061 atgtaggcaa attgtaagaa gaatacataa gcaagcacca gaaagtgttg tggtagttac
    12121 aggttgttat gcacaactta aaccaaaaga aattgccgaa ataccaggtg tagatttagt
    12181 tttaggtgct gcagaaaaat ttaatttagc tcaacatatt tcagaattta ccaaaggaga
    12241 cagcaccaaa atttgcagtt gcgatactag cgatattgca ggttttaatg cttctttttc
    12301 tgccaacgat cgtacaagaa cttttttaaa agtacaagat ggttgcgatt ataattgtag
    12361 cttttgcacc ataccaatgg caagaggtaa aagcagaagt gatagcatta gcaatgtact
    12421 aaatagtgct aaagaacttg ctagtaaagg agtaaaagaa attgttttaa caggtgtaaa
    12481 ccttggagat tttggcaaag gttcacaagg tgcaggagta tctataagca tgcaagaaag
    12541 agaagaagat ttttttgatt taatacaagc attagatgct atagatggta ttgaacgttt
    12601 cagaatatcc tctatagagc caaacttatt aaccaatcca ataatttctt ttgtggcaaa
    12661 aagtaaaaaa ttcatgccac attttcatat tcctttacaa agtggtagca accatatttt
    12721 aaaattaatg agaagaaggt atcaaagaga gttgtatcaa gatagagttg cttacattaa
    12781 atcacttatg ccacattgct ccataggtgt tgatgttatt gttggctttc ctggagaaac
    12841 ggatacatat tttgaagaaa catttaactt tcttcacaac ttagatgtta gttatttaca
    12901 tgtttttacc tatagcgaaa gagataatac tgtagcttta aatttaaagc ctgtaattcc
    12961 tgtaaatatt cgccatcaac gaaataaaac tttgcgtaat ttaagtttta tgaaactgca
    13021 atattttaca caacaacata gtgggcaaaa cagaaaagtg ttatttgaat ctgccgataa
    13081 aaatggaatg atggaaggct atacagataa ctacattaga atttctacac cattcagaaa
    13141 agaatgggaa aaccaaatta ttgattggaa aatataatgc tacaattaaa taatatagct
    13201 ttattacaat ttaaaaatta tactcaacag caatttgtat ttactcaaaa ggtagtaggt
    13261 atttgtggaa ggaacggctc gggtaaaact aatttattag atgcaattta ttacctgtgc
    13321 tttactaaaa gctacttctt agcttctgat gctcaaaatg tacaaatagg aaaagcagga
    13381 atgagaattg aaggaaacat gctattgaac aatactactc aaaatttaat ttgcatttta
    13441 agagaaaata ataaaaaaga atttttgtat aataaagagc aactaaaaaa attttcagaa
    13501 catattggta gatttccttg tgtaattatt tcaccagatg atacaacttt agtaacaggt
    13561 agtagcgaac ttaggcggaa atatatagat acaattattg ctcaaataaa ctctcattat
    13621 ttatacaatt tagtagatta caataaaata ttagttcaaa gaaatagttt actaaaacaa
    13681 gctgccgaag taggaacaat taataaggat gtagtagaaa tattaaatca acaacttgct
    13741 actaaagcaa attatatttt tgaagaacgt aaactatttt tcaagcaata cataccattt
    13801 gtgtacgatt attatagcaa aattgctagt aaaaccgata atattaccct ccatcatcaa
    13861 agtgcattat acaccgaaac tatagagaat ttattagaac aaaacctaca aaaagatatt
    13921 gctttacaac gtacaaccgc tggtattcat agagatgata tagaaataaa tatgaatggt
    13981 gcattattta aaactattgc atctcaaggc caaagaaaaa gtttgttgtt tgcattaaag
    14041 ttggcagaat ggcaaattat aaaacaaaaa aaattatact ctccaatatt attgttagat
    14101 gatgtttttg aaaaattaga tggtgaaaga atgcataact tattgcaatg ggtttgtaac
    14161 gaagaaagca cacaagtatt tataactgat actcataaag aaaggttaga acaagcattg
    14221 caaaatgctg caattaattt tcagttaata gaacttcaat aaaaaagagg agattttact
    14281 ctcctcctgg atacacatta ataataattt cacaatctgg taattgttgt tgcaacttaa
    14341 tatagtcttc ctgcggatac tgacaaggca aattcagcct gcgtaggttt ttcagctttt
    14401 ttaaacattt aatagattgt tccgttagta acccaagttg tctaaaattc attcttaact
    14461 ctaaagcctc taattgtttt actttactaa ggttacaaaa aaataactcc tgattaaaat
    14521 ctaacggaca accatttaat ctaaaagttc ttaggttgtt taactccatt aacttctcaa
    14581 aagaggcatc tggtgcagct ttgccttgct caatatcaaa caccattcct cgtgtataat
    14641 atacactaat ggtagaaata ttttcaggct ccttcaatgc ttcttttaaa gaagtagcat
    14701 aacctgttgg gttaaaacta ttttgtgcat ttactgccac aaccgagaaa agtaacatcc
    14761 atacgaaatg gatgtaatat tttacggact tcatagataa agggattagt aagcaatgta
    14821 aatgtaaaaa gcattttttg ttcaacttaa attttgggct tgttgtgcat aaagaaaaat
    14881 atttattgca attatgcaca ttaatataca tggtaccatc ttctaaaacc ataactaaat
    14941 ttttataatt aaataagtct ttacacaact attccccatt tatttttgta ttgaatattt
    15001 acaatagaac attgtttaat ttgcacttat gcaacaatac caacaactat tacaacatat
    15061 cattaatact ggagaaagaa aagaagacag gacaggtacc ggaacaatta gttgttttgg
    15121 atatcaaatg cgttttgatt tacaaaaagg ttttccgtta gtaaccacaa aaaaattgca
    15181 cttaaaaagt attatttacg agttactttg gtttttaaat ggcgatacaa acattaaata
    15241 tttaaacaat aatggcgtaa gtatttggaa cgagtgggca aatgacaatg gagaccttgg
    15301 gcctgtttat ggcaaacaat ggcgtagctg gcaaggtgca aatggtaaaa caatagatca
    15361 aatagccgat gtaattaagc aattaaaaac aaacccagat agtaggagaa tgattgtaag
    15421 tgcttggaat gtggcagaac tttctgaaat ggctctaatg ccttgccatg cactttttca
    15481 gttttatgtg gctaatggta aattaagttg tcagttatat caacgtagtg cagatgtatt
    15541 tttaggtgta ccatttaata tagcatctta tgcagcttta acaatgatga ttgctcaagt
    15601 ttgtaattta caattaggcg aatttataca tagctttggc gatgtacatt tatactgtaa
    15661 tcatattgaa caagcacaac tacaattaac cagaacaccg tttgagctgc caatattaaa
    15721 aattaactct tctgtaaaag atatttttaa ttttcaatac gaagatttta ttttagaaaa
    15781 ttatcagttt catccacata ttaaagcagt ggtagcagtt taataattaa ggttggttat
    15841 tattaagata aaataaaaat tttttcatga ttatttcatt aattgttgcc gctagtgaga
    15901 ataatgtaat tggtaaaaat aaccaactac tttggcactt acccaatgat ttaaaatttt
    15961 ttaaaaatac tacttggggt atgccagttg ttatgggtag aaaaacattt gagagccttg
    16021 gcaataaacc tttaaacgga cgattaaata ttgtaataac caaccaaaaa aaatggaatg
    16081 ccaatggtgt tgttgtagta aatagtttag acgatgccat ttttgtagca aaaagtcatt
    16141 cgtataaaga aatgtttata gcaggtggag gcttaatata taatttagtt atgaataaag
    16201 cagataaaat ttatttaacc cgtgtacaca ctgctattga aggcgataca ttttttccgg
    16261 aaataaatga aaaacagtgg caaaaagtac aagataactt ttgtaatgcc gatgaaaagc
    16321 atgcttttaa ctatagtttt caactttggg aaagaaaata acttcaaatt gccactaagt
    16381 ttaaaacttt agcattagta cagccaattt tattcaataa actgttatct tgccgccttt
    16441 aataaaagtt ttcaaaaaaa cctatgtcca tcactcaaat tcccaaattt ccggttgtaa
    16501 aagattcgtt acatgcggag ttaagaaaac gtgtacagca atattttgat gataaaggaa
    16561 taaaggcaac aggcaacata agcttattta ccaaagcaat tattttagtt actgcatttg
    16621 tactagttta tattcattta atatttttta caccacattg gtacatagcc ataccagagt
    16681 gtattatttt aggatgttta attgcagcaa ttggttttaa tgttatgcat gatggtagcc
    16741 atggcagttt tagtaaacac aaatggaaaa ataaattggc agcagcatct ttaagtatgt
    16801 taggtgcaaa tcattttatg tggaatatga aacacaacat gctacaccat agtttcacta
    16861 atgtagatgg agtagatgat gatattgaaa taggagtttt aatgcgtatg gcacccacac
    16921 aaaaacgata taaattacat aggtttcaac atttttattt ttggttttta tatatgttgc
    16981 tgtatgtttt ttggatattt tttacagatt atcataaata ttttacacaa aaaataggta
    17041 atgttcctct aaaaaaaatg gctactaaag atcatataga gttttggcta actaaaattt
    17101 atcatggctt tatatttatt gcattaccca tcataatggt tggctggttt tattggttaa
    17161 tgggttttct tgttgcaaca ttgggatctg gttttgtact tagcattgta tttcaattag
    17221 cacatactgt agagcatacc cactttccta ttgcagattt tgataacaac aaattacccg
    17281 atgagtttgc tgcacatcaa ataaaaacta ctgcaaactt tgctacaaaa aataaaatag
    17341 taagttggtt agttggaggt ttaaattttc agatagagca tcatttgttt cctaaaatat
    17401 cgcatgtgca ttaccctgca attagtaaaa ttgtaaaatc tgtttgtgca gaatatcagt
    17461 taacttatat agaatatcca acagtaagta gggcaattgt agctcatgta agatttttaa
    17521 aacacttggg taaatacgac taattgtact ttttactttt cagtaaatga caaaaaaaat
    17581 tattttagca ggtattgcaa cagttgtaat gatagtaatc atgcgtttgc tatcaatcaa
    17641 tttagttaca gattatagtc ccaacggaat tgtaagtttt gaattagcta aaagttatag
    17701 agatgcatca gccattatga tggcagttgg ggttaagcct ttacaaatta atgttgctgt
    17761 agattttgct tttattattg cttattgttt ctttctttat ttatgttgta aagctttact
    17821 taaccaatat accaaacaag ttttaaaaac tattggcttt atatttttag agctaagtat
    17881 ttttataggt atattagatt tagtagaaaa tatagccatg cttattacct taggaggtta
    17941 cggcactaaa ataagtgtaa gtgttagcaa atgggctgcc atagctaaat tttctattgc
    18001 tgggctggtt attgtttata ttctcattgc ttcaatttta ttgtattttt cttctagaaa
    18061 gaaagcctaa tgtttatacc taaaaaaaat attattgttg gaagaaaccc tgtagtggaa
    18121 gctttaaagc agcatttaac catagataaa atattagttt ccaaaaaagc atctggcgaa
    18181 attgtgaatg aaataaaaca acttgccaga gcaaacaatg tacctgttca atatgttcca
    18241 atagaaaaaa taaatagcct tactaacatt caacaccaag gcattgttgc atttaaaagt
    18301 gcagtgcgtt attttaacct acaacatgta atagattttg ttgtagcaaa aggagaaacc
    18361 cccttgtttt taattttaga tggcgttacc gatgtaagaa atattggagc aattgcacgt
    18421 actgcagtgt gttgtggtgc acaagcaatt ataattccag ataaaggcgt aggtgcttta
    18481 aatgaagatg cagttaaaac aagtgctggt gctcttgaac aaattcatat ttgcagagta
    18541 aatagtttaa tgattgctgt ggatgaacta aggctgaacg gtattagtgt ttttgccagc
    18601 gaaatgactg ctacaacaaa actgtacaat ttaaacctaa atataccttg tgcaattgta
    18661 atgggtagcg aagaaaaagg tgtgtatcct gcccttatga aaatttgtga aaacacattt
    18721 catataccta tggctaataa ttttgaaagc ttaaatgtta gcgttgctac cggtatgatt
    18781 ttgtatgaag caatgaaaca aagaaagtag tattatttta ttgaaacgtt attataattt
    18841 aattgcctgc ttttttacta ttgctataac cgtttttaat aagccactgt agtatttttt
    18901 ctctttgatc gccttgtata ataatttcat tattttttgc attgccacct gtaccacaaa
    18961 aattttttaa ttgtttcgct aatttttcta aatcggctaa cgtacctaca aaacctgtta
    19021 ttaatgtaac tgttttacca gctcgttgtt tagtatctaa ccaaattctt aatttttgta
    19081 aattaggtaa aagggtttta actgtttcct cctcattgtt aaaattaaag ttaggattag
    19141 tgctataaac aaatccatct ttatctgctt ttgatttttt attgctcata tagctaaaat
    19201 aaaaaaagca ttcaaaataa tattaagaat gctttaaaaa tatttaaaca acatgtaatt
    19261 atgctgttaa ttgtttaaag atatttattt agtcaattaa atctaaagaa taagcaaggt
    19321 aagtacatgt gccaggtagt gctaaccaaa accattgtct aaaatcgggt gtagctaata
    19381 aaaatactat gatagctaac caaaaaataa ataataaata gtacttgctt tttgattttg
    19441 ttttttccat tttcttcaaa tttttacaaa gatagggtta gaggaattag cttccaacgc
    19501 ttttattttt ttgtgcttct gtggcgtgta ttttatttgt atctttttta ggtttatcaa
    19561 aaaaaagcat ttttactgct acaataatca ttaaaaaagc aaatattttt tttacagtag
    19621 catcgggtag cgataaggct aatttacttc ctaaatatcc gccaactaaa aagcctatgg
    19681 ctaaaaatgg aattgctttt atatttacct cgcctgcttt atagtaataa attaacgaca
    19741 caataaatac aggtaataat aataagccta acgaagtacc ttgtgctgtt ttttgagaaa
    19801 aaccaagaat ataaattaat gctggtacaa taattattcc accaccaaca cctactaaac
    19861 cactaagcat gccagctgca acgccaataa taattaatat aataatagtg ttaactgtca
    19921 tagttttttt atttatttaa aaaagatttt ttataaaatt ctattgcttc tgaaacaatt
    19981 tttaatgcag tagttttatc accaaattct tctactttaa cctctttgtt ttctaatttt
    20041 ttatactctt caaaaaaatg ccttaactca tcaaaaaaat gtttgggtaa ttcttctata
    20101 ttattgtaat ggttaacacc tgggtcgtta gatgctactg caattatttt atcatcagca
    20161 tcgccactat ctatcatttg cattactcct acaacttttg cttccattag ggttaaagga
    20221 acaacaggaa tagaagttat tacaagtata tctagtgggt ctttgtctcc accataggtt
    20281 tgaggtataa aaccataatt acaaggataa taaaatgaag aataaattat tctgtctaat
    20341 tttaataagc cactgggttt atcaatctca tatttacatc tgctaccttg cggaatttca
    20401 ataactgcat ttacttttct tggtgcttgt tctcctgggt taacaccatg ccaaggatgt
    20461 tgaactaatt gttgcatatt ttaaaaatat aatttttatt taaaaataaa gaaaccaata
    20521 ctaacagcta ttaaggttaa aaaaaacgta agcccaatat ataaaaaaga tgctgcatat
    20581 tgttgctgtt gcattaactg aaatgtgttc cagctaaaag cagagaatgt ggtaaagcca
    20641 cctaaaaagc ctgtagtaat tattaagtac caatgctcca cattgtgtgt ttgttttgca
    20701 agaattgcca taataacacc aataaaaaat gagcctacaa catttactgt aatggtagca
    20761 taaggaaaat gtttgttagt aatcattaaa ccaataaaat atctaaaaat agaacctaaa
    20821 ccaccgccta tgccaactaa taaaatattt ttaagcatta taaattgggg ttaaatgtat
    20881 atttaaaatc tatgagccaa gtgttatttt ggtttacagc tttcactttt cttctctctt
    20941 tatcaaatga gttattgtaa taaataattg tttctgtggt agaaatagtt tctatagtaa
    21001 cattttgaat ggaggcttcg gataaaagtc ttttatcttt tttggtaaat tgataatatt
    21061 tatcttttgc ttctttcaat agtttgtcat tttccttatc gtgaagcata taaaaacttg
    21121 ctttctcaaa tttacctgct ttaaaagctt caataaattc tctggcagct tctaatggcc
    21181 tggttgctgg taaatatgta atgcttgaat tgttacaagc taaaagtaaa aaggcgataa
    21241 gtgaaaaaaa tatcttcatg gataacttta tgtaaattga aaatacaaaa cctagttcat
    21301 gatattcaaa caaaggtaag gttgattttg gtgaatagaa aaggcagctt ttaaagccgc
    21361 ctaatttata tgaatggtta gtaagtaatt ttttaacgag gatggttata ttttttttcg
    21421 tattcagctt cttctttttc aaattcttta tcgtatgctt taataatggc ttttactaat
    21481 ttatgtctta ctacatcttc ttcgtctaat tcaatatgag caataccatc aatatttttt
    21541 aaaattctaa cagctttttc taacccacta cgttggttgc gtggcaaatc tatttgtgta
    21601 gggtcgccag taataatagc ttttgcattg gcaccaatac gggttaaaaa catttttaat
    21661 tgtaagtcgt tagtattttg cgattcgtct aaaataataa atgcattatc taaagttctg
    21721 cctctcatat aggctaatgg ggcaatttca atagttctgg tagtcatata ataacctagt
    21781 ttatcggcag gtatcatatc gtctaatgca tcgtataatg gtcttaaata agggtcaatt
    21841 ttttctttta aatcacctgg taaaaaacct aagctttctc cagcttcaac ggctggcctg
    21901 gttaaaataa tttttttaac tactttattt tttaatgctc taaccgctaa tgcaactgct
    21961 gtatatgttt taccagtacc tgctggtcct attgcaaaaa caatatcgtt tttatcggct
    22021 gcagtaacca ttcttttttg gttggcagtt ctggctctta ctgttttgcc atttggccca
    22081 aaaactaaaa tatcgttagg gtttttgtcc acaaaattgt caatagtttc cgcatcatcg
    22141 ccacctaata tttgctcaaa atagttttca cttaaatgtc catttctttc taaatactga
    22201 acaattaaat ttattttttc ttttgcagag tctatttgtt ctggggcacc acttagttta
    22261 atatgtgttc ctctagatag aattttaagt aatggaaatt tttttttcag taaatcaaac
    22321 ttgccattgt ttacaccaaa aaattcaata ggattaatgg aatctagatt aatgattgta
    22381 tctgtcaaaa taataatggt tttaaataga ttaataattt gagttgagtt actactaacc
    22441 tttcctaccc aaatataatt ttaataattg tagatagtat taattattat tcacaaactg
    22501 tggataggtt ttacaacagt ttaagcaata agaaggaagc attttttgat taaaatataa
    22561 tactaaaatt tggggcatct aatatttcta tctgtattag gccttctaat ataaattaat
    22621 gagatttcta aaccgcctct tttttggctt gcatttttta aggaagaagt attaatatca
    22681 taggttgcac ctaatctaaa atcatcaaac tctaatccta tatatggtat taaggcatct
    22741 ttaaaacgta gccatgcacc agcataaaag ctagtaggtt ttggtgcttc gttgccactt
    22801 acattaaatt gtattgcacc accaacaaca gtttcggctg caccaccttg aaaactttgt
    22861 aatgcactaa catgtaaagt agtattatca ttcatgtcaa agtttcctcc accatgtatg
    22921 gtagtacgca tatttaataa atagttagca cctgtaaaag tttgctttgg tcttgtaaca
    22981 tggtatatac ttacgccagc ataataactg tttttatcgc tactgctacc tgtatataaa
    23041 acccctgcat ttaggtctat aaaatttgat tttagtgaag aagggttatt aaaaatttcg
    23101 gaagtaacac cagtaaaacc agaggtggta agttggtctt caaattttaa gcttgtagta
    23161 tttataagca tattg
//
LOCUS       DAOQHP010000002        21715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1085_length_21715_cov_4.349769, whole genome shotgun sequence.
ACCESSION   DAOQHP010000002 DAOQHP010000000
VERSION     DAOQHP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1085_length_21715_cov_4.349769"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLY81_00140"
     CDS             complement(<1..194)
                     /locus_tag="PLY81_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF28667.1"
                     /translation="MSSLVQEFNNYRSKMNDVILSKNNLVMKRLWNLDTNTYEDGALD
                     KKTKEMLGLVASMVLRCDDC"
     gene            complement(222..629)
                     /locus_tag="PLY81_00145"
     CDS             complement(222..629)
                     /locus_tag="PLY81_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF28668.1"
                     /translation="MKVLVITVGFIFCCFQLNAQAKGQSSKVDLDTIYNKVDVLPSYI
                     GGEEGWKKYLKKNTKYPKKAWWEELEADVTLEFTIKNDGSIVDIINLTVSGWGFEEEA
                     IRLLKKSGKWNPALKNGKPVHYHAKLTIPFRLK"
     gene            695..3850
                     /locus_tag="PLY81_00150"
     CDS             695..3850
                     /locus_tag="PLY81_00150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNF28669.1"
                     /translation="MKIIINLLMGKDTINKNFNDAYKSLNQEQKKAVDTIEGPILVNA
                     GPGTGKTQILATRIGNILLNADVLPNNILCLTYTDNGAVEMRKRLLSIIGSAAYNIQI
                     HTFHSFCNEVIQDNVTYFGKLNLEPISELEEIELFNKIIDTIPNNSALKRFSGDVYYE
                     KRRLQGLFALMKKEAWQPEHLLHCIDSYITELPTKEGFYYKRKYKQFNAGDAKIGAIN
                     AEIERMEILKEAVKLFPVYNKMMNENSRYNFDDMILWVLQAFNENNNLLLNYQERFLY
                     ILVDEFQDTSRSQNLLLQHLISYWDVPNVFVVGDADQSIFSFQDANVQNIESFINQYK
                     QHIVEIDLTTNYRSTQNILNTAYSLIKNNALRITKTLQPLEASDENRQKEDIVPTILE
                     FNTPAHEAIYIAKQIEELIKNKNVNGKDIAVIYRKHDIANEIANILQQKNIPINTKKK
                     VNILQLPFINNIIKILQWIDKENYLPYSGDDILFKILHSNFWNLKSLDIAKISVAVNN
                     ANKASKADKFSLRRWLNEMETKTADLFNQQKDELKSVINALESLLKESNNCTVQQLFE
                     KVIQTCGILSFIMQSNEKPWLMQVLTSLFNLIKEETKRNSDLTLSDLVKYLALMEKHN
                     ISPTVQKVINNTNGVNFITAHSSKGSEFEYVFVIGCTENVWEKGGGNNKVYKLPDNLV
                     HNNSIATNNEESRRLFYVAITRAKTHLQLSYFKYDVKDKAQVESSFIAEICNNSNITI
                     KPTEVSEDDFVAYTLLQFSESAIPEIELINNEYLDKILQNYTLSVTHLNNYLSCPIKF
                     YYQNLIKVPSAKNASMTFGTAVHYALETLFKNMIGNNNQFPSVQEFVDYFIYSMKKNK
                     EAFTAEEYKLKTDYGYKILPAYYNYHINNWQKIVKIEATFKNILVQNIPINGKLDKLE
                     FNGYNVNVVDYKTGKFANAKEKLIAPCDKEPNGGDYWRQAVFYKILIDNDKTNNWQVK
                     STQFEFVEPEKEKYEIERIDIKEEDVTTVKQQIIEVWNKIQAKEFNKGCGKPTCEWCN
                     FVKTNELAVALHTLIEEDETL"
     gene            3911..5317
                     /locus_tag="PLY81_00155"
     CDS             3911..5317
                     /locus_tag="PLY81_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNF28670.1"
                     /translation="MNKKTSLLLLALLFITINFLLISCANIIPPGGGPRDSLPPQLMQ
                     ALPKNFSTQFSSNKIVLSFNEYIELKEITKSLIVSPLPKNQPSVDYKLKTITIKLKDS
                     LIPNTTYSINFGNSIVDINEGNPLSNFTYVYSTGNTIDSCQLKGKVILAENGKIDSTL
                     IAVLYSNLSDTAVITQKPLYITQLKGDGSFTFKHIPPGIYAVYAMPNDYAKRYNDSTK
                     LFAFTDSAINVNYSNINSTTLYAFQEAKNNQSTPVASNNNIKNNNKAKHLKYTSTVEF
                     EKQDILNSTLELLFTSSINKLNTSKILLTDTNFNAYNNYTTALDSVSNKIILTTSWLA
                     NTAYKLLIYKDAVYNKQGNTIAQNDTLSFLTKKETDYGALKLRFNNLDTLKYPVLLIY
                     QQTNLVEAIKINKTIISRKLFKPGDFDLKILYDKNNNGIWNTGNYKNKLQPEIVVPLD
                     KKLSIRANWDNEAEINIK"
     gene            complement(5331..5504)
                     /locus_tag="PLY81_00160"
     CDS             complement(5331..5504)
                     /locus_tag="PLY81_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28671.1"
                     /translation="MSHSHFEGDSKKFTLTAILSFAIVFCFLALFSRCNGDYKTDKSA
                     GSHTPATKEHKAH"
     gene            5663..6406
                     /gene="ubiE"
                     /locus_tag="PLY81_00165"
     CDS             5663..6406
                     /gene="ubiE"
                     /locus_tag="PLY81_00165"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNF28672.1"
                     /translation="MTKFAHDAIVPDTNSQLTKKQQVAAMFNDIAFKYDFLNRFLSAG
                     IDIRWRKKGIKQLKELKPQFVLDVATGTADVALLTYKLLQPKKIIGIDISEGMLKIGK
                     QKIADKKLQQIIELEIGDSEQLNFTENSFDAITVAFGVRNFEHLEKGLENMLKVLKPG
                     GKLVIMECTQPKQTALKGFYKFYMNFITPGIGKMIAKNKQAYQYLNNSIQAFPEGETF
                     LNIMNKAGYKNTYLKTLSLGLCTIYCGSK"
     gene            6379..7092
                     /locus_tag="PLY81_00170"
     CDS             6379..7092
                     /locus_tag="PLY81_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF28673.1"
                     /translation="MYYLLRKQIILFGIFIFVITNVFAQKYVELNRPDRDELPYYFGM
                     TLAVNQSYLAATKHTKFINDDSILSVQPGASSGISLGLLGTLKLNYCWEFRFNPQLII
                     GGSKHFIYQKNYPVEEVKKTLPSTIVSFPFQFKLNSDRLNNFRTYVLFGAKYDIDLAS
                     NSSARNAQDMVKLKKSDFALEAGIGFNFFLPFVTISPELKFSYGLSNIHSRDPALKYS
                     NVFDKIQSRMISLSIHLED"
     gene            7208..7360
                     /locus_tag="PLY81_00175"
     CDS             7208..7360
                     /locus_tag="PLY81_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28674.1"
                     /translation="MKELIEKLSTEVGLTPEQANEAIEAIASFVKEKFPMLGGAVDSI
                     FSKEEE"
     gene            7836..9413
                     /gene="dnaB"
                     /locus_tag="PLY81_00180"
     CDS             7836..9413
                     /gene="dnaB"
                     /locus_tag="PLY81_00180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNF28675.1"
                     /translation="MEIISNLGRDNKKRRKSNIDLSTMVYGKVPPQARELEEAVLGAI
                     MLEKGAFDKVVDVNLKSECFYVEAHQHIYKAMQDMQQQSIPIDILTVVEELKRKELLQ
                     QVGGAYYITKLTNTVVSTANIDAHARIILQKFIQRELIRISGEIIGDAYEDSTDVFDL
                     LDDSETKMFNITNNYLKKNFEDIGSVLTTTINRIDYLRTREDDISGVPSGFSNIDSIT
                     FGWQPTDLLILAARPGVGKTAFALNLARNAALHPIKPTAVGFFSLEMSASQLVQRILS
                     AESEIPLQKISRGRLEEHEYQQLHTKGIKRLEQAPLFIDDTAALNIFEFRAKARRLVN
                     NHKVGLIIIDYLQLMSGSSENRNTNREQEISTISRNLKALAKELSVPIIALSQLSRAV
                     ESRKESKVPQLSDLRESGAIEQDADMVMFIYRPGYYEITANENGENIKDLAELKIAKH
                     RNGSLETLQLKMIPEIQKFEVWDRGDSGLGGNYKSLGPSPNNPSDSQNGGKFLYQTSK
                     MNNGNFDDGYDNEQPID"
     gene            9474..10715
                     /locus_tag="PLY81_00185"
     CDS             9474..10715
                     /locus_tag="PLY81_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF28676.1"
                     /translation="MIHFNKHTLHNGLKIIVHEDNSTPMAVVNIMYNVGARDEDPQKT
                     GFAHLFEHLMFGGSINIPEYDEPLQKAGGDNNAYTTNDLTNYYCVLPAQNIETAFWLE
                     SDRMLSLAFNKKSLNVQRKVVCEEFKEHYINKPYGDIWHNMRNLAFDVHPYKWMTIGK
                     ELSHIENATIEDVKAFFFKHYRPNNAIVVVAGNVQSNQIFQLVEKWFGNIPSSENSIK
                     NIPKEPQQNNAKFLELQANVPLDAIIKTWHIDARLTKGYYAADLITEIFGGGASSRLY
                     NSLVKNKKLFSSINCYHFGSIDPGLLTIEGKLIKGIKMQEAENAIEEELEKLKSTLIE
                     NNELQKVKNRTESMITFEDISVESRANSLAMYELLGDAAWMNTELEKYQEITIHDIQQ
                     YAQTIFNTKNSSTIHYYSKNN"
     gene            10750..12030
                     /locus_tag="PLY81_00190"
     CDS             10750..12030
                     /locus_tag="PLY81_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF28677.1"
                     /translation="MSFRKQAPAIQNAVDFDLKLPAIQQFALDNNIPVYSIHGGTQDV
                     IMIEFVFFAGNWYEEKNIIAAITNALLKNGTKTKNALAINEHFEFYGAYLNTHTYSET
                     ATVTLHTLNKNLEYLLPVLAELFTDCIFPEEELKTYIKNAKQRLEVNLKKCDFVANRL
                     IDEYIYGHNHPYGKYSTIADFDAIEKNDLQDFYKANYTNGKCMIFVAGKLPTNINNLL
                     NKYFGNLPFNYNKELQKNYLPVCNKTKKHSIINDTDGVQNAIRIARSFPNKIHPDYNN
                     AQILNTILGGFFGSRLMKNIREDKGYTYGIHSFIQNHFHQTAWMISTEAGKDVCNATI
                     EEVYKEMKLLCNEPISNDELFLVKNYLMGNLLSNQSGPFQVIAKWKSLILNNLKEDFY
                     YNSIHTIKTTNAKDLQEIANKYLNPADFYELTVL"
     gene            12048..12410
                     /locus_tag="PLY81_00195"
     CDS             12048..12410
                     /locus_tag="PLY81_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNF28678.1"
                     /translation="MQPFITKTLVTAAAVIIASFLLSGVHVDSSLTAIMVAIVLGLLN
                     TFIKPLLIILTIPITIFTLGLFLIIINILMIKWAASLVPGFTVENWWAAFWFSIIVSI
                     FSSIIQNITTTKNKSEEE"
     gene            12478..14301
                     /locus_tag="PLY81_00200"
     CDS             12478..14301
                     /locus_tag="PLY81_00200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNF28679.1"
                     /translation="MDKFIVSARKYRPQTFDTVIGQSHITTTLKNAITSNQLAHAFLF
                     CGPRGVGKTTCARILAKTINCENLQPDTEACNECNSCKSFNDGASLNIHELDAASNNS
                     VDGIRALVEQVRFAPQGGKYKIYIVDEVHMLSSGAFNAFLKTLEEPPAYAKFILATTE
                     KHKILPTILSRCQIFDFKRITTNDTVQHLQEIITKENINADKASLQVIAQKSEGCMRD
                     ALSILDKIVSFSNGEVTYTNTIEHLNILDHDYFFKIYQCLQNQDFATAMLLYDEINRK
                     GFEGDMLLSGFAEFIRNILVCKDEKSVKLLDVVVGLEDKYVTLAQQASLSYLISILNI
                     LNESELNYRMARNKRLHIELTLIKLNFLKQAIELTIDNEGSLVKKKIIHNNPVLKFKQ
                     IPSLQVKPKFASTTEEAKLYVQEPTIKQVKVTTPQQTENNTKTASTTTTSSNDIPPNN
                     INNSGNKRLLDILREKAGDKYNVTAILEAEGLVLEKLKTCWHNYALTLKQQGNKHSTV
                     KTFEIAELEIIDDLNFIIKVSAITQQKFIEQEKTNLLQSIHTAFNNRAINFTFQLTDT
                     LQQQDVPLHMKLNSKQKFEYLANKYPLLKELKEKLNLEIDY"
     gene            14347..15093
                     /locus_tag="PLY81_00205"
     CDS             14347..15093
                     /locus_tag="PLY81_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNF28680.1"
                     /translation="MEYNTQRNHLEMREYGRHIQKMVEYLLSIEDEEKRNNQAKAVIE
                     LMGFLNPHLRNVEDFKHMLWDHLFVISNFQIKVDSPYPIPQKETYKEKGERLPYPKRY
                     PRYAHLGKNLETVIDKALAEEDPEKKSGFANAIAYYMKLSYSNWHKELVHDDTIRAEL
                     DEITGGQLEFSNTPFIKHRNQNFERDDYGRGGSNQQRRQSFSNRNGNNNNGRNNNNNR
                     NNNNRNGNNNNGRNNNNNRNNNNRNFKKRY"
     gene            complement(15145..16464)
                     /gene="dacB"
                     /locus_tag="PLY81_00210"
     CDS             complement(15145..16464)
                     /gene="dacB"
                     /locus_tag="PLY81_00210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNF28681.1"
                     /translation="MKNSTLFFCRNKRLQIVVCIIGLLLIVNRGNTQAAVEKFINNSI
                     FASAHIGLAVYEPATDKYIYSYQSQKNFIPASNLKIFTCYAAIKNLEDSLTGLFYTQE
                     DSNFVVQFSGDPTLLHADFKQHPVIDFFTKNKFITVIKANWDNKYYGNGWAWDDFEAD
                     YMPELSTIPIYGNTVQFLKDENRVIVIPRWFKDSLTFYGNTQNGKYNIERNIHSNQFK
                     LVNSDQKFFQKNIPFITSDVLAIELLQDTIFKPIDYTDADVIGNVEWHKLKSQPTDTV
                     LKYMMQRSDNFYAEQLLLMIGNEKFKETKPNKIINSLLQNTFNGLPQKIRWEDGSGLS
                     RYNLVTPEDIVWVLTKMKNDIGAERVNTIFPTGNQGTLKGMFKNYTENIFAKTGSLSN
                     NFSLSGYIKTKSNKDLVFSILINNHQASTNEIKNAVEQFLINLIEKE"
     gene            complement(16481..17104)
                     /locus_tag="PLY81_00215"
     CDS             complement(16481..17104)
                     /locus_tag="PLY81_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNF28682.1"
                     /translation="MTNFIPIFPVSTVVYPNETINLHIFEPKYIQLINECFETNKVFG
                     IPVIINNTIAEMGTTLKVNSIIKKYDDGKLDIITEGVDVFSILEIIHIIPNKLYSGAI
                     VTYPENEKGLLTNKMKQIYKQVLALHKFLQVTKKFKREVEEMISYDIAHHICLSVEEE
                     YNLLQLLKENQRLEFLKRHLLKMLQTMQQTEKLKEKIKLNGHFRELR"
     gene            complement(17178..17903)
                     /locus_tag="PLY81_00220"
     CDS             complement(17178..17903)
                     /locus_tag="PLY81_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNF28683.1"
                     /translation="MNKPYLHTILSPELLNIYDINDSIVVIIDVFRATSTIATALYNG
                     AEKIIPVDSVEKCIAIGNATPNSITAGERDGKVIEGLQYGNSPAEYPRKFIEGKTLVL
                     TTTNGTKLLHMALNKGASEIITGSFPNLNAVCNFLRTQKKNIFLGCSAWKNRFNLEDT
                     LFAGAVIEKVKSSFHIHCDSSLMAEQMYLHHKHDMFAFARLTTHWHRLSKFGLDKDLE
                     YCVTNNVADVLPIYKNGSLIALT"
     gene            complement(17924..18286)
                     /locus_tag="PLY81_00225"
     CDS             complement(17924..18286)
                     /locus_tag="PLY81_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF28684.1"
                     /translation="MIKQILSYSLLVIILTSCSNYITKTGKASFYSDKYKGKKTANGE
                     TYKPNEFTAAHKKLPFGTKVKVTNLNNNKTVTVRINDRGPFVAGRIIDLSRVAASEIG
                     MINNGVVKVKIKYKRNKD"
     gene            18397..18469
                     /locus_tag="PLY81_00230"
     tRNA            18397..18469
                     /locus_tag="PLY81_00230"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18430..18432,aa:Lys,seq:ctt)
     gene            complement(18520..18924)
                     /locus_tag="PLY81_00235"
     CDS             complement(18520..18924)
                     /locus_tag="PLY81_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNF28685.1"
                     /translation="MNLVTKKIVMAKDIGIHGNLFGGILMAWIDEAAAAYATEYCHTP
                     NMVTVHVDAFTFKKPLKAGQHIRIYACVKELGRTSITLKLEAYKYNLYSREETLVCNT
                     NATFVRIDDDGTPTAIGETIRRNHYEKLPITT"
     gene            complement(19054..20163)
                     /locus_tag="PLY81_00240"
     CDS             complement(19054..20163)
                     /locus_tag="PLY81_00240"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNF28686.1"
                     /translation="MNRKQLTIGLFGFGCIGKGLYEVLQKTPALKASIKSICVKTKNK
                     QRSLSNQYFTFEKNDILQDDTINTIVELIDDADAAFEIVKTALQKGKAVVTANKKMIA
                     EHFEELLHLQKQYNVPLLYEAACCASIPIIRNLEEYYDNDLLESIEGIVNGSTNYILT
                     KTVEKNISFSLALKEAQQKGFAETNPLLDVGGFDAKYKLQILLAHAFGVITQPQNIFN
                     LGINNLYDFELNYAKEKGYKIKLVAYASKNEEGKIISFVLPKFVTSKDKLFSVDDVFN
                     GVKIQSYFCDNQFFVGKGAGAYPTSSAVLSDISALTYDYRYEYKKLNTQHSLANEEDI
                     FLKIFFCVFPYQLILSLNHFLKILKNTISMKTMAL"
     gene            complement(20173..21339)
                     /locus_tag="PLY81_00245"
     CDS             complement(20173..21339)
                     /locus_tag="PLY81_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNF28687.1"
                     /translation="MKETTKILHGIPVDELTGAIATPIYQTSTYVQIAPGVHKGYDYA
                     RSNNPTRKVLEDLIASLEEGKNGFAFASGLAAIDAVVKLLQFGDEIIAVEDIYGGAFR
                     LFTHIYQKFGIHVNYVDSTNALNIENAITPKTKLIWIETPTNPTLKISDIQAIAKIAK
                     ANKVLLCVDNTFASPAVQKPIAFGADIVIHSATKYLAGHSDLIAGLVVTNSEELGEKI
                     KFIQNATGAILAPFDSWLVIRGIETLPLRVKQHSETALKIATFLQQQDIVNNVYYPGL
                     QEHKNYDIAKKQQKYFGGIISFDLKIEDKILATQLVSNTSYFKLAESLGGVKSLICLP
                     CEMTHKSIPLEKRHQSGVTDSLIRLSVGLEDAEDLINDLKTVFQTIAKKISIKA"
     regulatory      complement(21521..21634)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         8189 a         3168 c         3129 g         7229 t
ORIGIN      
        1 atacaatcat cgcatcttaa aaccatgctt gcaactaacc caagcatttc ttttgttttt
       61 ttatccaatg ctccatcttc ataagtattc gtatctaaat tccataaacg tttcattact
      121 aaattattct tacttagtat tacatcattc attttagaac gatagttgtt aaactcctgc
      181 actaaactgc tcatatatat gtgttgaaaa attaaacaaa attattttag tctaaaagga
      241 attgtaagtt ttgcatgata gtgaacaggc ttaccatttt ttaaggcagg gttccatttt
      301 cctgattttt ttaacaaacg aatagcctcc tcttcaaaac cccagcctga tacagttaaa
      361 ttaattatat ctacaatact gccatcgttt ttaatagtaa actctagtgt tacatcagct
      421 tctaactctt cccaccaagc ttttttgggg tactttgtat ttttctttaa atattttttc
      481 cacccttctt ctccacctat ataactaggt aaaacatcaa ctttattata aatggtatct
      541 agatctactt tagatgattg cccttttgct tgtgcattta attgaaagca acaaaaaata
      601 aatccaactg ttattactaa tactttcata aacaaaataa aatttcaatt aataacgtaa
      661 accaattaaa aatcttacct acaaataagt aaatttgaaa ataataatta atttattaat
      721 gggtaaagat acaataaata aaaactttaa cgatgcgtat aaaagtttaa accaagagca
      781 gaaaaaagct gtggatacaa ttgaaggccc tatattagta aatgcaggac caggtacagg
      841 taaaacacaa atactagcta cacgtattgg taatatttta ttaaatgccg atgttttgcc
      901 taataatatt ttatgcttaa cctatacaga taatggagca gtggaaatgc gtaaaagatt
      961 attaagcatt attggtagtg ctgcgtacaa cattcaaatt catactttcc atagtttttg
     1021 caacgaagta attcaagata atgtaaccta ttttggaaaa ttaaatttag agccaattag
     1081 tgaattagaa gaaatagaat tatttaataa aataattgat accataccta ataacagtgc
     1141 tttaaaaaga tttagtggcg atgtatatta cgaaaaaaga aggcttcaag ggctatttgc
     1201 tttaatgaaa aaagaagcat ggcagccaga gcatttattg cattgtattg atagctatat
     1261 aactgaatta ccaactaaag aaggttttta ttataaacga aaatataaac agtttaatgc
     1321 aggcgatgca aaaattggtg ctattaatgc cgagatagaa agaatggaaa ttttgaagga
     1381 agctgtaaaa ttatttcctg tatataataa aatgatgaat gaaaacagca ggtacaattt
     1441 tgatgatatg attttatggg tgctgcaagc atttaatgaa aataataatt tattattaaa
     1501 ctaccaggaa aggtttttat atattttggt ggatgaattt caagatacaa gccgttctca
     1561 aaatttatta ttacaacatt taattagtta ctgggatgtt ccaaatgtat ttgtagtagg
     1621 agatgcagac cagagtattt tctcctttca agatgccaat gttcaaaata tagaatcttt
     1681 tattaaccaa tacaaacaac atattgtaga aattgattta accaccaact atcgttcaac
     1741 acaaaatatt ttaaacacag cctattctct tattaaaaac aatgctctaa gaattacaaa
     1801 aacacttcaa cccttagaag catctgatga aaataggcaa aaagaagata ttgtacctac
     1861 tattttagaa tttaatacac ctgcacacga agcaatttat atagctaaac aaattgaaga
     1921 attaataaaa aataaaaatg ttaatggaaa ggatattgca gttatttata gaaaacatga
     1981 tattgcaaat gaaattgcta atattcttca acaaaaaaat ataccaatta acactaagaa
     2041 aaaggtaaat attttacaac taccttttat aaataatatc attaaaatat tacaatggat
     2101 agataaagaa aattatttac cctatagtgg agatgatatt ttatttaaaa ttttacacag
     2161 taatttctgg aatttaaaaa gcttagatat tgccaaaata agtgttgcag ttaataatgc
     2221 caataaagca agtaaagcag acaaattttc tttgcgtagg tggttaaatg agatggaaac
     2281 aaaaactgcc gatttattta atcaacaaaa agatgaactt aaatcagtaa ttaatgcttt
     2341 agaaagcctt cttaaagaaa gtaataattg cacagttcaa caactatttg aaaaagtaat
     2401 tcaaacctgt ggaatactta gttttattat gcaaagcaac gaaaagcctt ggctaatgca
     2461 ggttttaacc tctttgttta atttaataaa ggaagaaacc aaaagaaatt cagatttaac
     2521 cctttctgac cttgtgaagt acttagcact gatggaaaaa cataatatta gtccaactgt
     2581 tcaaaaagtt atcaataata ccaatggagt aaattttata acagcacaca gtagtaaagg
     2641 aagtgagttt gagtatgttt ttgtaattgg ttgcactgaa aatgtttggg aaaaaggtgg
     2701 tggcaataat aaagtataca aattacccga taatttagta cacaacaaca gcatagcaac
     2761 aaacaatgaa gaaagcagaa gactatttta tgtagcaata actagggcaa aaacacattt
     2821 acaattaagt tattttaagt atgatgtaaa agataaggca caagtagaaa gtagttttat
     2881 tgcagaaatt tgtaacaact ccaatatcac aattaagcct acagaggtta gcgaagacga
     2941 ctttgtagcc tatactttat tacaattctc ggaaagtgca atacccgaaa ttgaattaat
     3001 aaacaacgaa tatttagata aaattcttca aaattatact ttaagtgtta cccatttaaa
     3061 caattattta agctgcccaa ttaagtttta ttatcaaaac ttaattaaag tgccctctgc
     3121 taaaaatgca agtatgacat ttggtactgc agtgcattat gcactggaaa ctttgtttaa
     3181 aaatatgatt ggcaataaca accaatttcc atcggtacaa gagtttgtgg attattttat
     3241 ttatagtatg aaaaaaaaca aagaagcatt tacagcagaa gagtataaat taaaaacaga
     3301 ttacggctat aaaattttac ctgcttacta caattaccat atcaataact ggcaaaaaat
     3361 tgtaaagata gaagcaactt ttaaaaatat actagtgcaa aatattccta ttaacggaaa
     3421 gttagacaaa ttagaattta atggttataa tgtaaatgta gtagattata aaaccggaaa
     3481 gtttgcaaat gccaaagaaa aattaattgc accttgcgat aaagaaccaa atggaggaga
     3541 ttattggagg caagcagttt tttataagat tttaatagat aatgataaaa caaacaattg
     3601 gcaagtaaag agtactcagt ttgaatttgt agaaccagaa aaagaaaaat atgaaataga
     3661 aaggatagat ataaaagaag aagatgtaac aactgtaaaa caacaaataa tagaagtatg
     3721 gaataaaatt caggcaaaag aatttaataa aggttgtggt aagcctactt gcgaatggtg
     3781 taattttgta aaaacaaatg agctagctgt ggctttacat actttaatag aagaggatga
     3841 aacattatga tttttattgc atccatacct attaagtttg caaaatatta attgttgtag
     3901 cattttatac atgaataaaa aaacttctct ccttctatta gcattgttat tcattactat
     3961 caacttttta ttaattagtt gtgctaatat tattcctcca ggtggtggac caagagattc
     4021 tttacctcct caactaatgc aggctcttcc aaaaaatttc tctacacaat ttagtagcaa
     4081 taaaatagtt ttatctttta atgaatatat tgaactgaaa gaaataacaa aatctttaat
     4141 agtttctcct ttacccaaaa atcaaccaag tgttgattat aaactaaaaa caataacgat
     4201 aaaactaaaa gatagcttaa tacctaacac cacatattcc ataaattttg gtaacagtat
     4261 tgttgatatt aatgagggta accctttaag taattttact tatgtttact caacaggtaa
     4321 tacaattgat agctgccaac taaaaggtaa agttatttta gcagaaaatg gtaaaatcga
     4381 ttctacctta attgctgttt tatatagcaa cctttcagat acagcagtaa ttactcaaaa
     4441 gccactttac atcactcagt taaaaggaga tggtagtttt acttttaaac atataccacc
     4501 aggtatatat gcagtttatg caatgccaaa cgattatgca aaaaggtaca acgatagtac
     4561 aaaacttttt gcctttaccg atagtgcaat aaatgtaaac tatagcaata ttaattctac
     4621 aacattatat gcttttcaag aagcaaaaaa taatcaaagc acaccagttg caagtaataa
     4681 taacataaaa aataacaata aggctaaaca cttaaaatat acttctactg ttgaatttga
     4741 aaaacaagat attttaaatt caaccctaga gttacttttt acaagctcta ttaataaatt
     4801 aaatacttct aaaattttat taaccgatac taactttaat gcttacaata actacactac
     4861 tgcgttagat tcagtaagca ataaaataat tttaacaaca tcctggttgg caaatactgc
     4921 ttataaatta ttaatttata aagatgctgt ttataacaaa caaggcaata caattgcaca
     4981 aaatgacaca ttaagttttt taacaaaaaa agaaactgat tatggtgctt taaaattaag
     5041 gtttaataat ttagacactt taaagtaccc agtgttatta atttaccaac aaactaacct
     5101 ggtagaagct attaaaataa ataaaactat tatcagcaga aaattattta agccaggcga
     5161 ttttgattta aaaattttat acgataaaaa taataatgga atttggaata ccggaaatta
     5221 taaaaataaa ctacaacctg aaatagtagt tcctttagat aaaaaactat ccattcgtgc
     5281 caattgggac aatgaagcag aaattaatat aaaataatca ataattactt ttaatgagct
     5341 ttatgctctt ttgttgcagg tgtatgactt ccagcagatt tatcagtttt ataatcgcca
     5401 ttacaacggc taaataatgc taaaaaacaa aacacaatag caaaagataa aattgctgtt
     5461 aaagtaaatt tcttactatc tccttcaaaa tgtgagtgcg acataatgtt gtttttttcg
     5521 ccagcaagat atacaaattt tttaaactat acttttttgc ataatatttt aatttataaa
     5581 tctaaaacca cttttacata aaatttttgt tttgttaata cctgcccagc tttatgctca
     5641 atccttatct tcgcaaataa ctatgacaaa gtttgcacat gatgctatag tgccagatac
     5701 aaacagccag ttgactaaaa aacaacaggt tgctgctatg tttaatgata ttgcttttaa
     5761 gtacgacttc ttaaataggt ttttaagtgc aggaattgat attagatgga gaaaaaaagg
     5821 tattaaacaa ctaaaagaat taaagccaca atttgtttta gatgtagcta caggcactgc
     5881 agatgttgca ttacttacct acaaattact tcaaccaaaa aaaattattg gtatagatat
     5941 tagcgaaggc atgttaaaaa ttggcaaaca aaaaattgcc gataaaaaac ttcaacaaat
     6001 tattgaacta gaaataggcg atagcgaaca attaaatttt actgaaaaca gttttgatgc
     6061 tattactgtt gcttttggtg taagaaattt tgaacattta gaaaaaggat tagaaaacat
     6121 gttaaaagtt ttaaaaccag gcggcaaatt agtaattatg gagtgtaccc aaccaaaaca
     6181 aacagcatta aagggttttt ataaatttta tatgaacttt ataacaccgg gaattggtaa
     6241 aatgattgca aaaaacaaac aagcttacca gtatcttaat aattcaattc aggcttttcc
     6301 agaaggtgaa acttttttga atattatgaa taaggcaggt tataaaaata cttatttaaa
     6361 aacattgagt ttaggattat gtactattta ctgcggaagc aaataatatt atttggcata
     6421 tttatttttg taataacaaa tgtatttgcc caaaaatatg tagagttaaa tcgtccggat
     6481 agagatgaac taccctatta ttttggtatg accttagcag ttaaccaaag ctatttagct
     6541 gctactaaac atacaaaatt tattaacgat gacagtatat tatctgttca accaggggca
     6601 agtagtggaa tttctttagg tttattgggt actttaaaat taaactactg ttgggagttt
     6661 aggtttaacc cacagcttat cattggtggt tcaaaacatt tcatctatca aaaaaattat
     6721 cctgtagaag aggttaaaaa aacactccct tctaccattg ttagttttcc atttcagttt
     6781 aaattaaatt cagacaggtt aaataatttt agaacctatg tactatttgg tgctaaatac
     6841 gatattgatt tagcaagtaa ttctagtgcc agaaatgcac aagacatggt aaaattgaaa
     6901 aaaagcgatt ttgcactcga agcaggtatt ggtttcaact tttttttacc atttgtaacc
     6961 attagtcccg agcttaaatt tagttatggt ctaagtaata ttcatagcag agaccctgca
     7021 ttaaaatatt caaatgtttt cgataaaatt caatctagaa tgatttccct ttctatacat
     7081 ttagaagatt agttgtatct tcctacatta aaatacaact ataatattaa ttagcttaag
     7141 ctattaaatt tatagttatt tcataaaaaa attctactaa aaacaataac acaaaaaata
     7201 aaattatatg aaagaactaa ttgaaaaatt aagtacagaa gtaggcttaa caccagagca
     7261 agccaatgaa gcaatagaag ccattgcaag ttttgtaaaa gaaaaatttc cgatgttggg
     7321 cggtgctgtt gatagtattt ttagcaaaga agaagagtaa aatgtttatt aaatagaagt
     7381 ttaattaaat aacatcttct tcaacttgtt actattatta tagctgaaaa atttaaagac
     7441 accaaagtgt ttttaagcca taatgtacaa aaaataactt gccaatttag gcaacattag
     7501 ttcaattatt gttctaattc atttacatta attctactta aaaacatata tttcttctat
     7561 aaatctttct gcacaatatg tgtgcataaa ctagtttttt attgtaacaa cttttgtaaa
     7621 ttgtgcataa atagcaagtt tactgttagt ataattaaat ttttattgaa ttaaattgtg
     7681 aatagctgtg aattaataaa tgctgcctaa aaagatttta atactccatt tctgtagcat
     7741 attcgctaac actatttatt tttttaaaaa aattataaaa aaaacagatt acaaaacaca
     7801 ataacaaacc ccttaaattt ttgttttatt aacccatgga aataattagc aacttaggaa
     7861 gagataataa gaaaagacga aaatcaaata ttgatttaag tacaatggta tatggcaaag
     7921 taccaccaca agcaagagag ttagaagaag cagtattagg tgcaattatg ctagaaaaag
     7981 gtgcatttga taaagtggtg gatgtaaatc ttaaatcaga atgtttttat gtggaagctc
     8041 accagcatat ttataaggca atgcaagata tgcagcaaca aagcattcca atagatattt
     8101 taacagtagt agaagaactt aaaagaaaag aattattaca acaagttggc ggtgcatatt
     8161 atataaccaa attaaccaat acagttgtaa gtaccgcaaa tattgatgct catgcaagaa
     8221 taattttaca aaaatttatt caaagagaat taataagaat aagtggcgaa ataattggcg
     8281 atgcttatga agacagtaca gatgtgtttg atttattaga tgacagcgaa accaaaatgt
     8341 ttaacataac caataactat ttaaagaaaa attttgaaga tattggaagc gttttaacaa
     8401 ccacaataaa tagaatagat tatttaagaa ccagagaaga tgatattagt ggcgtaccaa
     8461 gtggcttttc taatatagat agtataacct ttggatggca gccaaccgat ttattaatac
     8521 ttgctgcaag gccaggtgtg ggtaaaactg cttttgcgtt aaaccttgca cgcaatgcag
     8581 cacttcatcc aatcaagcca actgctgttg ggttttttag tttagaaatg agtgccagcc
     8641 aattagtaca acgtatatta agtgcagaaa gtgaaatacc actccaaaaa atttcaagag
     8701 gccgattaga agaacacgaa taccaacaac tacatacaaa aggtataaaa agattagagc
     8761 aagctccttt atttattgat gatacagccg ccctaaatat ttttgaattt agagcaaaag
     8821 caagaagatt agtaaataat cataaagtag gtttaattat aatagactac ttacaattaa
     8881 tgagtggctc ctctgaaaat agaaatacaa acagagagca agaaatttct accatatcca
     8941 gaaatttaaa agcactagca aaagaactaa gtgtaccaat tattgcatta tctcagttaa
     9001 gccgtgcagt agaatctcgt aaagaaagta aagtaccaca attaagcgat ttacgtgagt
     9061 ctggtgcaat agaacaagat gcagatatgg taatgttcat ttatcgtcca ggctattacg
     9121 aaataacagc caacgaaaat ggagagaata ttaaagattt agccgaatta aaaattgcaa
     9181 aacatagaaa tggctcctta gaaactcttc agctaaaaat gattccagaa attcaaaaat
     9241 ttgaagtttg ggacagaggc gacagcggtt taggaggaaa ttataaatct ttaggaccat
     9301 cgccaaataa cccttcagat tcacaaaatg gaggaaagtt tttataccaa acaagcaaaa
     9361 tgaataatgg caattttgat gatggctacg ataatgaaca accaattgac tagaatttat
     9421 ttaattcaac tcttattcta taactatatt ctttcaactt ttactaaagt ttaatgattc
     9481 attttaataa acatactcta cataatggac taaaaataat tgtacacgaa gataacagca
     9541 ccccaatggc tgttgtaaat attatgtaca atgttggtgc aagagacgaa gacccgcaaa
     9601 aaacaggctt tgcacattta tttgagcatt taatgtttgg cggaagtata aatattccag
     9661 aatatgatga gcctctacaa aaagctggtg gagacaacaa tgcttataca acaaacgatt
     9721 taacaaatta ttactgtgta ttgccagcac aaaatattga aactgcattt tggttagaga
     9781 gtgatagaat gctgagtttg gcttttaata aaaaaagttt aaatgtacaa cgtaaagttg
     9841 tttgcgaaga gtttaaagaa cattatataa acaaacctta tggcgatatt tggcataata
     9901 tgagaaacct tgcctttgat gtacatcctt ataaatggat gacgataggt aaagaattaa
     9961 gccatattga aaatgcaact atagaggatg tgaaagcatt tttctttaaa cattatcgtc
    10021 ctaataatgc aattgtagta gtggcaggca atgtgcaaag caaccaaatt tttcaattag
    10081 tagaaaaatg gtttggcaat attccttcct cagaaaatag cattaaaaat atacctaaag
    10141 aaccccaaca gaacaatgca aaatttttag aattgcaagc caatgttcct ttagatgcaa
    10201 ttataaaaac atggcatata gatgctcgtt taaccaaggg ttattatgct gcagatttga
    10261 taaccgaaat atttggaggt ggtgcatcct caaggctgta caacagttta gtaaaaaata
    10321 aaaaactttt ctcctctatt aattgctacc attttggttc tatagatcca ggtttactaa
    10381 ctatagaagg taagttaatt aagggcatta aaatgcaaga agcagaaaat gcaattgaag
    10441 aagaacttga aaaattaaaa tctactttaa ttgaaaacaa tgagctacaa aaagtaaaaa
    10501 acagaacaga aagtatgata acatttgaag atataagtgt ggaaagcagg gcaaatagct
    10561 tagctatgta cgaactttta ggagatgcag catggatgaa taccgaatta gagaaatatc
    10621 aagaaattac tattcatgat attcaacaat atgctcaaac aatttttaat acaaaaaata
    10681 gtagtactat acattactac agcaaaaaca attaatttaa attatagttt ttaaatttta
    10741 attacaacaa tgtctttcag aaaacaggca ccagctatac aaaatgctgt agattttgat
    10801 ttaaaattac cggcaataca acaatttgct ttagataata atataccagt ttattccatc
    10861 catggtggta cacaagatgt aataatgatt gaatttgttt tttttgcagg taattggtac
    10921 gaagaaaaaa atattatagc agctattaca aatgcattac taaaaaatgg tacaaaaacc
    10981 aaaaatgcat tagcaataaa tgaacatttt gaattttatg gtgcttattt aaatacacat
    11041 acttacagcg aaacagcaac tgtaacttta cacactttaa acaaaaactt agagtattta
    11101 ttacctgtac ttgcagaact ttttacagat tgtatctttc ctgaagaaga attaaaaacc
    11161 tatattaaaa atgccaaaca acgattagaa gttaatctta aaaaatgcga ttttgttgca
    11221 aacagattaa ttgatgaata tatttatggg cataaccacc cctatggaaa atattctaca
    11281 attgccgatt ttgatgcaat agaaaaaaat gatttgcaag atttttacaa ggcaaattac
    11341 accaatggaa agtgtatgat ttttgttgca ggaaaactgc caaccaatat aaataactta
    11401 ttgaacaagt attttggtaa cttgcctttt aattataata aagaattaca aaaaaattat
    11461 ttacctgttt gtaacaaaac taaaaaacac tctataataa acgatacaga tggagttcaa
    11521 aatgcaatta gaattgctag aagttttcct aataagatac atccagatta taacaatgct
    11581 caaatattaa ataccatttt gggtggcttt tttggaagca ggttaatgaa aaatattaga
    11641 gaagataaag gttacaccta tggcatacat tcttttatac aaaatcattt ccatcaaacc
    11701 gcatggatga taagtacaga agcaggaaaa gatgtttgta acgcaactat tgaggaagta
    11761 tataaagaaa tgaaattatt atgtaacgaa ccaataagta acgacgaact ttttttagta
    11821 aaaaattatt tgatgggaaa tttacttagc aatcaaagtg gcccttttca agttattgca
    11881 aagtggaaat cattaatatt aaataattta aaagaagact tctattataa ttcaattcat
    11941 accattaaaa caactaatgc aaaagatttg caggaaatag caaataaata tttaaatcct
    12001 gcagattttt atgaacttac tgttttgtaa ttataaaaaa taaaatcatg caaccattta
    12061 taaccaaaac attagtaaca gcagcagcag taattattgc ctctttttta ttaagtgggg
    12121 tacatgtaga tagtagttta actgcaatta tggtagccat agttttaggt ttactaaata
    12181 cttttattaa accattatta ataatattaa caataccaat taccattttt acacttggtt
    12241 tatttttaat tatcatcaat atattaatga taaaatgggc agcaagttta gtacctggtt
    12301 ttactgtaga aaattggtgg gctgcatttt ggtttagtat tattgtttct attttttcgt
    12361 ccattattca aaatattaca acaacaaaaa ataaatcaga agaagagtag ataaatattt
    12421 tggtgtaaat tatagttgaa tagttcaatt aaaaataaat ctgctaaatt gccaactatg
    12481 gataagttta ttgtttctgc cagaaaatat cgaccacaaa catttgatac tgtaattggg
    12541 caatctcata ttacaacaac tttaaaaaat gcaataacca gcaaccaact tgcacatgca
    12601 tttttatttt gtgggccacg tggtgttggt aaaacaactt gtgcaagaat tttagccaaa
    12661 acaattaatt gcgaaaactt acaaccagat acagaagcct gcaatgaatg taatagctgt
    12721 aaaagtttta atgatggggc ttctttaaat attcatgaat tagatgctgc cagtaacaac
    12781 agtgtagatg gtattagagc attagtagag caggtacgtt ttgcaccaca aggaggcaag
    12841 tacaaaatat atattgtaga tgaggtgcac atgttatctt ctggtgcatt taatgctttt
    12901 ttaaaaacat tagaagagcc gccggcgtat gcaaaattta ttttggccac aaccgaaaaa
    12961 cataaaattt taccaacaat tttaagtcgt tgtcaaatct tcgactttaa aagaataact
    13021 acaaacgata ctgtacaaca cttacaagaa ataattacaa aagaaaatat taatgcagac
    13081 aaagcgtcgt tacaagttat tgctcaaaaa agcgaaggct gtatgagaga tgctttaagt
    13141 attttagata aaattgtaag cttttcaaat ggcgaagtaa cttataccaa cacaattgag
    13201 catcttaaca ttttagacca tgattatttt tttaaaattt atcaatgctt gcaaaatcaa
    13261 gattttgcta cagcaatgtt attatatgat gaaataaata gaaaaggttt tgaaggagat
    13321 atgcttttat ccggctttgc cgaatttatt agaaatattt tggtgtgtaa agatgaaaaa
    13381 tcggtaaagc tattagatgt tgttgtaggg ttagaagaca agtatgtaac actggcacaa
    13441 caagcaagcc tatcttattt aataagcata ttaaatattt taaacgagtc tgaactaaac
    13501 tatagaatgg ctagaaataa aaggttacat atagaactta cacttataaa acttaacttt
    13561 ttaaagcaag caattgaatt aactatagat aatgaaggta gccttgttaa aaaaaaaata
    13621 atccacaata atccggtttt aaaatttaaa caaataccct ctttacaagt aaagcccaaa
    13681 tttgcttcaa caacagaaga agcaaaattg tatgtacaag aacctacaat aaaacaagtt
    13741 aaagttacca caccacaaca aactgaaaat aatactaaga cagcatctac tacaactact
    13801 tcttctaatg atataccacc aaataatatt aataattcgg gcaataaaag attgttagat
    13861 attttaagag aaaaagctgg tgataaatat aatgttacag caattttaga agcagaaggt
    13921 ttggtattag aaaaacttaa aacttgttgg cataattatg cattaacatt aaaacaacag
    13981 ggaaataaac attcaacagt taaaactttt gaaatagctg aattagaaat tatagatgac
    14041 ctaaatttta taattaaagt ttcggccatt acacaacaaa agtttattga acaagaaaaa
    14101 acaaacttat tacaaagtat tcatacagca tttaataata gagcaattaa ctttactttt
    14161 caattaacag atactttaca acagcaagat gtacccttac acatgaagtt aaatagcaaa
    14221 caaaaatttg aataccttgc taataaatat ccattactta aagaattgaa agaaaaatta
    14281 aacttagaaa tagattacta aaaggtaatt taaatttatt tatatatcaa cccctacatt
    14341 tgcaatatgg agtataatac acaaagaaac catttagaga tgagagaata tggtaggcat
    14401 attcaaaaaa tggtggagta tcttttgtca atagaagatg aggagaaaag aaacaatcag
    14461 gctaaagctg ttatagaatt aatgggcttt ttaaaccctc atttaagaaa tgtagaagat
    14521 tttaaacaca tgctatggga tcatttattt gtaatcagca attttcaaat taaagttgat
    14581 agcccttacc caattccaca aaaagaaact tataaagaaa aaggagaaag attaccttac
    14641 ccaaaacgat atccaagata tgctcattta ggtaaaaatt tagaaactgt tattgacaaa
    14701 gccttagctg aagaagatcc agaaaaaaaa tctggttttg ctaatgctat tgcctattat
    14761 atgaaactat cttatagtaa ttggcataaa gagttagtac atgatgatac cattagagca
    14821 gagttagatg aaattactgg tggtcaacta gaatttagta atacaccatt tataaaacat
    14881 agaaatcaaa actttgaaag agatgattat ggacgtggag gaagcaacca gcaacgaaga
    14941 caaagtttta gcaaccgaaa cggaaataat aataacggaa gaaataataa taacaaccgt
    15001 aacaacaaca accgaaacgg aaataataat aacggaagaa ataataataa caaccgtaac
    15061 aacaacaacc gaaattttaa gaaaaggtat taatacattt tttcaaaaag gcactttaaa
    15121 taaagtgcct tttttatttt ggttttactc tttctctatc aaattaatca aaaactgctc
    15181 tactgcattt tttatttcat tagtagatgc ttgatgatta ttgattaata ttgaaaatac
    15241 aagatcctta ttagatttag tttttatata tccgcttaaa gaaaaattat tgcttaatga
    15301 gcctgttttg gcaaaaatat tttctgtata atttttaaac attcctttta aagtaccttg
    15361 gttgccagta ggaaaaatag tatttactct ttctgctcca atatcatttt tcatttttgt
    15421 aagtacccaa actatatctt ctggagttac taaattatac ctactcaaac cacttccatc
    15481 ttcccatcta attttttgag gtaagccatt aaaagtattt tgtaataaag aattaataat
    15541 tttattaggt tttgtttcct taaatttttc attccctatc atcaataaaa gttgttctgc
    15601 ataaaagtta tcgcttcttt gcatcatgta ttttaaaaca gtatcggttg gttgagattt
    15661 taatttatgc cattcaacat tcccaattac atcggcatct gtataatcaa ttggtttaaa
    15721 aattgtatct tgtaataact ctatagccaa tacatctgat gtaataaatg gaatattttt
    15781 ttgaaaaaat ttttgatcac tattcactaa cttaaactga ttagaatgaa tgtttctttc
    15841 aatattatat ttaccatttt gcgtattacc ataaaaagtt aaagagtctt taaaccaacg
    15901 tggaataact attactctat tttcatcttt caaaaattgt actgtattgc catatatggg
    15961 aatagtagaa agttcaggca tgtaatcagc ttcaaaatca tcccaagccc agccattacc
    16021 ataatatttg ttatcccaat ttgcttttat aacagtaatg aatttgtttt tagtaaagaa
    16081 gtcaattaca ggatgttgct taaaatctgc atgaagtaaa gttgggtctc cggaaaactg
    16141 tacaacaaag ttgctatctt cttgtgtata aaataagcct gttaaactat cttctaaatt
    16201 ttttattgca gcataacaag taaaaatttt taggttgctt gcaggtataa aatttttttg
    16261 agattggtaa ctataaatat atttatctgt tgctggttca taaacagcca atccaatatg
    16321 tgccgatgca aatattgaat tattaataaa tttctctaca gcagcctgtg tattacctct
    16381 attaacaatt agcaataaac caataataca tactacaatt tgtaacctct tatttctgca
    16441 aaaaaatagt gtactgtttt tcatacttaa attttatttg ttatctcaac tctctaaaat
    16501 ggccattcag ttttattttt tctttcaatt tttctgtttg ttgcatggtt tgtagcattt
    16561 ttaataaatg cctttttaaa aactctagcc tttggttttc tttcaacaat tgtaataaat
    16621 tatactcttc ttcaaccgat aaacaaatat gatgtgcaat atcataactt atcatttcct
    16681 ctacctctct cttaaatttt tttgtaactt gtaaaaactt atgcaatgcc aaaacctgtt
    16741 tgtaaatttg tttcatttta ttggtgagta agcctttttc attttcagga tatgtaacaa
    16801 tggctccact atataatttg ttgggtataa tatgaattat ttctaaaatt gaaaaaacat
    16861 ctactccctc tgttattata tctaattttc catcatcgta tttttttata attgaattta
    16921 ctttaagcgt tgtgcccatt tcggcaatag tattattgat aattacagga ataccaaata
    16981 ctttatttgt ttcaaaacat tcattaatta attgtatata ctttggctca aatatatgta
    17041 ggttaatggt ttcgttagga taaacaacgg tactaacagg aaatatagga ataaagtttg
    17101 tcatgcatta aaagtaatta tttgaataaa aaagagccta ctattaaatg aaatgaaaac
    17161 ttaaaatagt atatgaatta agttagtgca attaacgagc catttttata aattggtaac
    17221 acatctgcca cattattagt aacacaatat tctaaatctt tatctaaccc aaatttgctt
    17281 agcctatgcc agtgtgtggt taaacgtgca aaagcaaaca tatcatgctt atgatgtaaa
    17341 tacatttgtt ctgccattaa actactatcg caatgaatat ggaaagaaga ttttactttt
    17401 tcaattacag caccagcaaa taaagtatct tctaaattaa atctattctt ccatgcacta
    17461 cagcctaaaa aaatattttt tttctgtgtt cttaaaaaat tacaaacagc attcaagttt
    17521 ggaaaagagc cagtaataat ttcgcttgca cctttattta atgccatgtg tagtagtttg
    17581 gtgccattag ttgtagttaa cacaagcgtt ttaccttcaa taaattttct aggatattct
    17641 gcagggctat tgccatactg taatccttca attactttgc catctcgttc gccagcagtt
    17701 atactgtttg gtgttgcatt accaatggct atacattttt caacagaatc tacaggaata
    17761 attttttctg caccattata caatgcagtt gcaatagtag aggttgctct aaatacatct
    17821 attattacaa caatgctatc attaatatcg taaatattta atagctcagg cgataaaata
    17881 gtgtgtaaat agggtttatt catgctgcaa atatattgtg ctattaatct ttgtttcttt
    17941 tgtatttaat tttaactttt acaacaccat tatttatcat tccaatttca ctagcagcca
    18001 ctctgctaag gtcaataatt ctgccagcta caaatgggcc tctatcgttt atacgtacag
    18061 ttaccgtttt attgttattt aaattagtaa cttttacttt agtaccaaaa ggtaattttt
    18121 tgtgtgctgc agtaaattca ttgggtttat acgtttctcc atttgcagtt tttttgcctt
    18181 tatatttatc tgaataaaaa gaagccttac cagttttagt aatataatta ctacaagagg
    18241 ttaatataat taccaataaa ctataagata aaatttgttt tatcataagt taaaactaaa
    18301 aaagcatact aaattttagt agattaaatt ttcaacaaat acaaaattat tgttgaaaac
    18361 aaaattgcac cttaacttta tttagaaaaa ttttcagact ccttagctca gttggtagag
    18421 caactgactc ttaatcagta ggcccagggt tcgagtccct gaggggtcac aaaagttaaa
    18481 cgaaattaaa ggtgcaaagc aacttaatta ataaaactgt tacgttgtta ttggtaattt
    18541 ttcataatgg tttcttctta ttgtttcacc aattgctgtt ggtgttccat cgtcatcaat
    18601 tcttacaaaa gttgcattag tattgcaaac aagtgtttct tctctgctgt acaagttgta
    18661 tttgtatgct tctagtttta gtgtgataga agttcttcca agctctttaa cacaagcata
    18721 aattctaata tgctgccctg cctttaaagg ttttttaaaa gtaaaagcat ctacatgtac
    18781 agtaaccata ttcggtgtgt ggcaatattc tgtagcataa gctgctgctg cttcatctat
    18841 ccaagccatt aaaattccac caaataaatt tccgtgaatg ccaatatctt ttgccattac
    18901 aattttttta gtaactaagt tcatattctg ttttttgatt gttaattgtt tggtaattga
    18961 aatctctttt aataatatca aaaagtatga ttgaagtttc tatatttctt attgcaacaa
    19021 tttctttaat acattttaga gagcaagtgc ctattataaa gccattgttt tcattgaaat
    19081 agtattcttc aatattttta aaaaatggtt taaactcaaa atcaactggt aaggaaaaac
    19141 gcaaaaaaat atttttaaaa atatatcttc ctcatttgct agtgaatgtt gagtattaag
    19201 ttttttatac tcataacggt aatcataagt taatgctgaa atgtctgata atactgctga
    19261 tgatgttgga taagcacctg cacctttacc aacaaaaaat tgattatcgc agaagtatga
    19321 ttggattttt acaccattaa aaacatcatc taccgaaaat aatttatctt tacttgttac
    19381 aaattttggt agtacaaacg aaattatttt accctcctca ttctttgagg cataagcaac
    19441 caacttaatt ttgtagcctt tttcttttgc ataatttaat tcaaaatcgt aaaggttgtt
    19501 gatgcctaaa ttaaaaatgt tttgtggttg agtaattact ccaaaagcat gagctaataa
    19561 aatttgcaat ttgtatttgg catcaaaccc accaacatca agtaaaggat tggtttctgc
    19621 aaagcctttt tgttgtgctt cttttaatgc taatgaaaat gaaatatttt tttcaactgt
    19681 ttttgtaaga atataattag tagaaccatt tacaattcct tctatagatt ccagtaaatc
    19741 gttatcgtaa tattcttcta aatttctaat aataggaatg ctagcacagc atgcagcctc
    19801 atacaacaat ggtacattat actgcttttg aagatgaagt aattcttcaa aatgctcagc
    19861 tatcattttc ttatttgcag ttacaacagc ctttcctttt tgcaaagctg tttttacaat
    19921 ttcaaatgca gcatctgcat catcaattaa ttcaactatt gtattaattg tatcatcctg
    19981 taaaatatcg tttttttcaa acgtgaaata ttggttagat aaagaccttt gtttattttt
    20041 agtttttaca caaatgcttt taatgcttgc ttttaaggca ggtgtttttt gtaatacttc
    20101 atacaaacct ttaccaatac acccaaaccc aaaaagccct atggttaatt gttttctatt
    20161 cattttttta aattaagctt tgatggaaat tttttttgct attgtttgga atactgtttt
    20221 caaatcatta attaaatctt ctgcatcttc taatcctaca gacaaacgaa ttaagctatc
    20281 tgttacacca gactggtgtc ttttttctag tggaatagat ttgtgtgtca tttcgcaagg
    20341 caagcatatt aaacttttta caccacctaa actttctgct agtttaaaat atgatgtatt
    20401 gctaactaat tgtgttgcta aaattttatc ctctattttc aaatcaaaac ttattatacc
    20461 gccaaaatat ttttgttgct ttttagcaat gtcataattt ttatgttctt gtaaccctgg
    20521 gtaataaaca ttatttacaa tatcttgttg ttgtaaaaat gtagcaattt ttaaggctgt
    20581 ttcagaatgt tgcttaactc taagaggtaa agtttcaata ccacgaataa caagccaact
    20641 atcaaaagga gcaagaatag caccggtagc attttgaatg aatttaattt tttctcccaa
    20701 ttcttcgcta ttggttacaa ccaatcctgc aattaaatca ctatgccctg ctaagtattt
    20761 tgttgcagaa tgaattacaa tatctgctcc aaatgcaatt ggtttttgta ctgcaggaga
    20821 agcaaatgta ttatcaacac aaagcaatac tttattggct ttagcaattt ttgcaatagc
    20881 ttgtatatct gaaattttaa gagttggatt agtaggagtt tcaatccaaa ttaatttagt
    20941 tttaggagta atggcatttt ctatatttaa tgcatttgtt gaatctacat aatttacatg
    21001 aataccaaat ttttgataaa tatgtgtaaa taaacgaaag gctcctccgt aaatatcctc
    21061 aacagcaatt atttcatcgc caaattgtaa tagttttact actgcatcta tagcagccaa
    21121 gccactagca aaagcaaaac cattttttcc ttcttctaaa cttgcaatta aatcttctaa
    21181 aacttttctt gtagggttat tacttctggc atagtcgtac cctttgtgta cacctggtgc
    21241 tatttgcaca taggtagatg tttgataaat tggggtagca attgcacctg ttaattcatc
    21301 aacaggtatg ccgtgcaaaa tttttgttgt ttctttcatt tttgttttga attaatttgt
    21361 taaacatttt attactgttc atcaaaaaaa atctaatcca aaacacagat agaatacaac
    21421 ttgtggaaga tctttataat aaaaaagctc ttccaacaag ttagaagagc ctttaatatt
    21481 atttgccgtt ttttaacggt tattaattgc tatattttct aacttatctt tctcggcatt
    21541 actgctgagt tgaatttggc acctttcttt gcttgaggca aagaggttgt caagacgtca
    21601 tcgggtcaat tccctctgtc tttctggata agaatatttt taaagaacta agtgcaaaag
    21661 aaagtataaa aattaaactt ccaaattttt ttttaaaaaa gaaatgtacc tcttt
//
LOCUS       DAOQHP010000003        19704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1244_length_19704_cov_3.995267, whole genome shotgun sequence.
ACCESSION   DAOQHP010000003 DAOQHP010000000
VERSION     DAOQHP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19704
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_19704_cov_3.995267"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLY81_00250"
     CDS             <1..1119
                     /locus_tag="PLY81_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28688.1"
                     /translation="YKGWDPYDGLNSKIFQAIPFIKNNRFCRLAWIQFFKKSPINFRK
                     LLLVEKGYNSKGIALFLSAYCRLYTIEPTEKNVQKIKELSTKLLEMKADGWSGYCWGY
                     NFDWQARAFFQPKNTPTVVASVYASWALLEAYEILQDKKLLDAAESCCSFILKDLNRN
                     YDADGDFCFSYSPLDKSIVFNASLLGSRLLSKMYSLTKNELYKIEAKKSVSYCVKHQQ
                     SNGAWAYGLQSFHYWIDNFHTGFNLECIAAYQKYTGDTSFETAISKGFEYYINNFFEE
                     DGKPKYYNNKTYPIDIHAPAQLVITLYELGLIQKEEKLCSTVLNWTLQNMQDNNGYFY
                     YQINQSLNSKIPYIRWAQAWMFFALATYKKAFHENRIV"
     gene            1100..1837
                     /locus_tag="PLY81_00255"
     CDS             1100..1837
                     /locus_tag="PLY81_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNF28689.1"
                     /translation="MRIELFNCPIDAITMHQTLNKIENAIIQKQHLHHVVVNVAKLVH
                     MQNNSELRESVVNCDIINADGQGVVWASKILGKKIPERVAGIDLMQNLVQLAHDKKYK
                     IFFFGAEEHIVKEVVNKYTNQFSPSIIAGYRNGYFNKEEESEIANEIANSGANILFVA
                     MGSPKKENFLFNYKQVLQKLNFVMGVGGSFDVVAGKVKRAPVWVQKIGMEWFYRMAQE
                     PRRLFMRYFTTNLALIKLILKEKLKKG"
     gene            1845..3239
                     /locus_tag="PLY81_00260"
     CDS             1845..3239
                     /locus_tag="PLY81_00260"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNF28690.1"
                     /translation="MHHKYAAFIRFLNFIYDLAILNIILLFLFLRFSPSVINVSDLLF
                     NKVYFFNWLNINFAWIAATIFTNVYTLRNLIYFKEGVKNSFNGLAIFILFVVCINFFL
                     SSVVFFSKKDLLIFIILFSTFFWLARLVFYYFKKRYNLELYAGKRILLIGDESAIDEV
                     KKTFYEKKELGFVYAGHLPDSEFHANNQKYIENLLASIQLQNIDEVFFAKSSIKDEEL
                     YNLIQTLDKNTIRVRVIPDFFRFYTKFKNLSFHGNLPLLSLREEPLQNILNRLLKRFF
                     DFIFSLLVIILLASWLFPLLALLIKLESRGPVFFKQLRSGKDNKQFWCYKFRSMRVNV
                     NADVIQATKGDQRITRIGKFLRKTSIDELPQFLNVFIGNMSVVGPRPHMLKHTEQYAA
                     MVDKYMIRHFVKPGITGWAQVHGYRGEISNVEDIEKRVEYDIWYIENWSLALDIQVVL
                     LTVYNIIRGEENAH"
     gene            3220..4860
                     /locus_tag="PLY81_00265"
     CDS             3220..4860
                     /locus_tag="PLY81_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28691.1"
                     /translation="MKKMLTKTYFLKYVLLAILVICKFNVAIGQAKFPVKIQNKLYAQ
                     LYEKWARENNIYSIAALQPYCNTDIRFNKELLSFYQLPSDSLRKKRNFFTQTFRNGNM
                     AEFVSDKSTVTINPLFQFEFGKSKDYSVYHFNRGIQLDANFNNKVFISSSIYENVSKF
                     PKYINQYIDSLTVVPGMGKARYNTNGKNIEYALPIGFIGYKPNKNFYFELGNNKNFIG
                     NGYRSLLLSDVAYSYPYFKMQTHISKFTFQTIWSQFVDASKNWVNTNGYDKKFGAFNT
                     VSYTGIKNVELNLFQSVIWSNKDSLGNRRDQEWGYFVPIIFFNSLNFNNGSPDNSLIG
                     VDASYTIKKHTVIYGQLIIDDFNIRESKNGKGFFQNKYGVQLGVKSFKPFNLDNAFAR
                     IEFNTVRPYTYASKTPTLSYTHYGEVLAHPFGANFREFLLEANYKIKRYLFSANLIIA
                     WYGADDANSHWGKNIYKSDYLSQTGIFSFNNKITQGIKTNLIYSNVAIRYLMNPVTNS
                     GIAAQIFYRTEKSNLINNKDFIFNISYSTNLLNILKEF"
     gene            complement(4857..5258)
                     /locus_tag="PLY81_00270"
     CDS             complement(4857..5258)
                     /locus_tag="PLY81_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28692.1"
                     /translation="MNKTFKHIIAISLILIVCWWGYTNTEEINKISNVHKPFEQQLKR
                     IIWLLIVGIITWWAFAKDNNQWLSKIVIIIYAFVIITIGLIGVLEWKFKFLSENWREF
                     FAGVRLFLSSPLPFIFLWIMRKINFDHFSKN"
     gene            complement(5251..5796)
                     /locus_tag="PLY81_00275"
     CDS             complement(5251..5796)
                     /locus_tag="PLY81_00275"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNF28693.1"
                     /translation="MANSFFQTIPSAVKKFLLRSIVFFIIWKLIYHLVLYPIRVPDKQ
                     LTHITAVITKEMIQFHYPNDKLNIEEINYPSPKENLLKNNKKIVGIADGCNGLELYIL
                     YIGFLACFPSNKKRLFAYAITGIVLIFVLNNIRSYVITLLNIHNSSFTDVAHHYFFKI
                     FIYAVMFLLWVKYTKPKLKDE"
     gene            5888..6553
                     /locus_tag="PLY81_00280"
     CDS             5888..6553
                     /locus_tag="PLY81_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNF28694.1"
                     /translation="MSKNNFSFGFNQNSFTNEQLQYKALQINGSLAKRFFDIAVSICF
                     LLFIFSWLYPIIAIAIKLETKGLAMFVQDRIGLNGKIFKCYKFRTLLSNSNSLDKDSL
                     PLSYTTPATKVGVFLRKYNLDELPQFINVLKGNMSIIGPRPHAVPFHNLYSEYVPNIH
                     QRLLVKPGITGLSQINGLRGDVEDIEQNKKNIQQRVQCDTNYIFHWSFKLDLKIFVKT
                     FIG"
     gene            6558..6992
                     /gene="dut"
                     /locus_tag="PLY81_00285"
     CDS             6558..6992
                     /gene="dut"
                     /locus_tag="PLY81_00285"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNF28695.1"
                     /translation="MQINIINKSTNPLPTYETKGASGMDVRANINEAITLQPLQRALI
                     PTGIFIEIPQGYEAQIRPRSGLAIKQGITCLNTPGTIDADYRGEIKIILINLSNETVT
                     IQHGERIAQMVFCKIEQAELVQVQIINETTRGTGGFGHTGKQ"
     gene            7002..7835
                     /locus_tag="PLY81_00290"
     CDS             7002..7835
                     /locus_tag="PLY81_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNF28696.1"
                     /translation="MNKQLLVGCTLLLVVIFSCRTTKNTHNIQTAITKKDTTQVVTVA
                     EEKSIDSFAVVKEILNQTINHKINFSTFYAKIKVDYESPSDSRKLTAYTYMQKDSVIY
                     IRLVGSFLGITKEGVVAKITKDSVTVINKIDKIVQKRTIAYLQEVTQIPFDFSTIQDV
                     IIGNPVYIDSNIVSYRNSLDDFSVLMLGNLFKHLITLDKNDFKVLHSKLDDIDVFKNR
                     TCNITYSDYVDAFGVKFSTKRKFSVSEKTKLDVWLDFKQYTFNEPLTYMFNIPKNYKK
                     Q"
     gene            8001..9386
                     /locus_tag="PLY81_00295"
     CDS             8001..9386
                     /locus_tag="PLY81_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNF28697.1"
                     /translation="MKNKPSFIVLTKISPKALFNAAKFFVAIAFVLVASFQLHAQTKE
                     ELQKKEDELRKEINELSKNLVQIQKNKKVSLAQVASVQRKIAAREELIRTINRRVRLL
                     DETIYQNELEIYRMRKELDTLKQQYARSIAFAYKNRGNYQYLNFLFSSTSFNDALKRV
                     TYLKSYRKLRETQVGNIVKTQQVLQQKIGALTNSKNEQNQVLESQKTQLVDLEADKKE
                     KNDVVKGLKGQEKEVTALLRKKEKQRAEMRNTIAAIIRREIAEAEKARLAKLKAETDA
                     RNKANAANKNIAKTTTTKPGVANTEVVEEMASTKTVNREYSALESTAEGVEMSVNFEK
                     RNLNWPVSIGTVIAPFGNYQITEKLQGHSDGIEIAVPQGTAVKSVADGEVTYVGDFGG
                     SPAVIVKHGKYFTIYNYLSEITARKSQQVKAGTVLGKSGISTFGEESLLFMVTNDKTV
                     FLNPRNWLKSR"
     gene            9467..10723
                     /locus_tag="PLY81_00300"
     CDS             9467..10723
                     /locus_tag="PLY81_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNF28698.1"
                     /translation="MEEVYNLIWLFCSIIIIGFFAGYEIAFVSTNKLSVELKKKQGKK
                     SGIVIAKFLEKPAEFIGSCLIGLNFFYVIYGLAFNQFLQGSFWSFFHVKNGYLQLAIN
                     TLIAGIVILVLGEFFPKAIFRAKNDMLISVFAPLANIFHSLFAPIARFFVSVSQWVLK
                     YLFNIKMSEKEEAFSHIDLEHFFQQAKEHDESGEELNKELFENALSLPTVKIRQCLVP
                     RTEIEGIDIESNIEEVKKIFIQTKLTRLIVFKDNIDNILGYIHQLDLFKKPKNIQDVL
                     LPIPVIPEAMNAIDLISKFTKERRNIAWVVDEFGGTAGIVTLEDILEEIFGEIHDEYD
                     TDDFVAKQLSENEFVLSGRLELDMLAEKYKLTFDVDDSETLSGYIINFYGSIPNQRES
                     IIIGNYQFDILSVSDTRIENVKLKVL"
     gene            10841..13642
                     /locus_tag="PLY81_00305"
     CDS             10841..13642
                     /locus_tag="PLY81_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF28699.1"
                     /translation="MPVTESIETKNNQLLAQCYEQLINNNINDALSNITNLIIQYTNA
                     AMCCISVNRDKANKLYHCANNDQIIFSENKVQQFFKQFKEIQPTHSSFNLRSNSNENI
                     SLFANSPIKQILGYSFNHNNNELILLAFKEPVEENIYSSILQKLSTAISKVASTTTIQ
                     EELFFRNDVYESTLYTLNQIIWDHNLKTDTTHVMGFAHRIGNFIPQKTFQYNIKDWLL
                     DKAHPDDMQRVMQNYEQFILQKNNTVNEIKFRMFSPTANEYFWVQTRQTLLYDETGEP
                     ALVVGTTIDIQDTYQIAADLEKHKIQNLLLVESLQESNEKLLTILNSSKEIIFTIDLQ
                     TSQFENVNKAISLLGYTPKEWIGQNYTQWSLDKRKKFHNLLKHASASNNSVKTQQISF
                     TTKDEKTEIPFEFSTSVFTFKNKKYLLCILRDISERVAYENSIKQVTEQLAYLINNID
                     DVYAIYNLQQKKYEFVSNNIEYLYGCSKDTYLSNDLYWKEIIFKDDYASIESQMNDII
                     SNKSKGEFFYRINTPVGETKMLLEKVTVTTDKDGNADKLFIVKSDYTNLENAEQNLIE
                     SERKFRFISENITDFISIIDIDGRFMYASPSSVKVIGYTTEELIGKNIIFFIHAEDVT
                     NFLEEALEKIVFNKEEASLRYRLNKKDGSFNWVETYFKPIIDANNDTTSIICSTRDVT
                     EREQLMKELQQSLNKERALNELRAQFVSMASHQFRTPLTVIQSGIELMQIYLEGISEQ
                     KQQPFKKQFLRIESEVSRLQDLMNDVLLLGRAEANRTPYNPTFGNIIQFCRDIIESRY
                     NNQDYNNRKVILYIFGTAQEVMFDAKLLDHTLENILSNAFKYSNKGDIKFTITFSETT
                     VNIAVKDQGIGIPANEIENLFQPFYRATNATEFEGTGLGLSIVNEFIETHGGKLFVES
                     EIDKGTTVNIQLPLYQIKK"
     gene            13655..14767
                     /locus_tag="PLY81_00310"
     CDS             13655..14767
                     /locus_tag="PLY81_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF28700.1"
                     /translation="MSFTTKILVIEDEDNIRENIQELLEAKQYLVRTANNGRQGVLEA
                     IDFRPHLILCDIMMPKMDGYKVLEYIRKTSIIQNVPFIFLTAKIDRNDIRQGMELGAD
                     DYLTKPFTAKDLVTAIETRLKKQQKIIDEYAKVKHELDTSAFASYYHEFNTPLHGIIG
                     GINLLLSSRTSFNDTQIQELLYSILKSGLRLNHSLANLMLFEEIKRAEVHTELLSMFN
                     SGNSSGKWADRIKTELEVIAREIYNRPEDLVFDMQATDVHISPEYLMRILLELTDNAL
                     KFSSKGSKISIKGKPDGDNYFIEIKDSGKGFAIENNNQIAPFKQFNRSKYEQQGLGIG
                     LYLVKRLVEINSGEIKIESEENKGTTITILLPSFGI"
     gene            14801..15841
                     /locus_tag="PLY81_00315"
     CDS             14801..15841
                     /locus_tag="PLY81_00315"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNF28701.1"
                     /translation="MYKLIRDILFCFPPENVHYFSMNMLKMLCSNKAIKNLISNQFQF
                     QQTTLQKEVFGLQFKNPVGLGAGFDKNALYLNELNALGFGFVEIGTVTPLAQNGNDRP
                     RLFRLPKDKALINRMGFNNDGVHEIKKRLIHWRNQPSLQSNQLIIGGNIGKNKITPNE
                     NAWKDYEICFNELFELVDYFVVNVSSPNTPGLRELQEKDALRKILAHIQNQNQGKTMP
                     KPLLLKIAPDLTQEQLDDIIDLGIEIKLDGLVATNTTIDRQGLQTNAQKIQQIGAGGL
                     SGKPVALKSTQVVKYLAQQTQNQIPIIASGGIFTAQDAKEKLQAGASLIQVFTGFIYQ
                     GPSIVKNICKEL"
     gene            complement(15853..16275)
                     /locus_tag="PLY81_00320"
     CDS             complement(15853..16275)
                     /locus_tag="PLY81_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HNF28702.1"
                     /translation="MKRIIFFILIILTIKTNAQTTDEAAIRQILQTQEKAWNNGNLDA
                     FMFGYWNNDSLMFIGKKGLTYGYHATLANYKKSYPDTAHMGKFSSTIISIKKLSNEYY
                     FVIGKWELFRTVGNISGHYSLLFRKIKGKWVIIADHSS"
     gene            16429..>19704
                     /gene="ileS"
                     /locus_tag="PLY81_00325"
     CDS             16429..>19704
                     /gene="ileS"
                     /locus_tag="PLY81_00325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNF28703.1"
                     /translation="MKKYKEFEGLNLPVIEKEILQQWQQEKAFEKSISLREGSTPFVF
                     YEGPPSANGMPGIHHVISRTLKDLVCRYKTMQGFQVKRKGGWDTHGLPVELGVEKELG
                     ITKEDIGKKISVAEYNQKCREAVLRYKKEWDDITTKMGYWVDLKEPYVTFENNYIESL
                     WWLLKKLYNRGLLYESVSIQPYSPAAGTGLSSHELNQPGTYKDVKDTSATILFKVKEN
                     EKNKFQNIEGNIFFMAWTTTPWTLPSNLGLTVGANIDYVLVKTFNPFTHKIIHVIIAK
                     ALLGKYFKAEDENGDFENYQPENKKLPWQIIATFKGSELENVEYEQLLPFSANTLSVI
                     KEQTPNAKPFRVLLGDFVTTEDGTGIVHTAPAFGADDFRVGKKYDIGILTMVDKRGKF
                     LTGIEDGIFLYGEEYVKEAYLTEAEKETEFKKQKQILETNEKVKDLKKYLSVDDRIVL
                     KLQEEGKLFKKEKYDHNYPHCWRTDKPILYYPLDAWFIKTTALKDRMVELNKTINWKP
                     KSTGEGRFGNWLENMVDWNLSRSRYWGTPLPVWCTEDGDEQICIGSIAELEANLHPNS
                     PVKNITDLHKPQVDEVILQSSRGKEMKRVPDLVDVWFDSGAMPYAQWHFPFENVKTFT
                     ENYPADFIAEGVDQTRGWFYTLHAIGSLIKEDIIEELKNQGYSENTIEQVKGVAYKTV
                     VSNGLVLDKNGNKMSKRLGNVVNPFDTIEKFGADATRWYLITNASPWDNLKFDIEGIK
                     EVQRKFFGTLYNTYQFFVLYANVDGFAFEEKYIPLADRPEIDRWILSSLNTLIEKCTS
                     AMNDYEPTASGRLIEEFTDEHLSNWYVRLSRRRFWKGEYEHDKICAYQTLYECLETIM
                     QLIAPISPFFSDAVFQNLNAVTKRFNASSIHHVSYPTSNANAIDKDLEERMQLAQNTC
                     SLVLSIRKKSNIKVRQPLQKIMIPVLNPTMKIQLEKVEDLIKSEVNIKDIQYLTATEG
                     VIKKKAKANFKTLGTKLGANMKDAANIISNFSQQQIAELEKSGSLNIQLSNSEYQLTV
                     SDVEIVAEDIPGWSVASNSSVTVALDITITEALQSEGDAREFVNRVQNIRKESNFELT
                     DRIF"
BASE COUNT         7564 a         2641 c         3183 g         6316 t
ORIGIN      
        1 tataaaggct gggaccctta tgatggacta aacagtaaaa tatttcaagc aataccattt
       61 ataaaaaata acaggttttg tcgtttagct tggattcaat tttttaaaaa atctcctatc
      121 aattttagaa agctattatt agttgagaaa gggtataata gtaaaggaat agcacttttt
      181 ttatctgcat attgtagatt atataccatt gaaccaacag agaaaaatgt acaaaaaata
      241 aaagagcttt caactaaact gcttgaaatg aaagcagatg gttggagtgg ttattgttgg
      301 ggttataact ttgattggca ggcaagggca ttttttcaac ctaaaaatac acctactgtt
      361 gttgcatctg tttatgcctc atgggctttg ttagaagcgt atgaaattct acaagataaa
      421 aaattgttag atgcagcaga aagttgttgc agttttatat taaaagacct taacagaaat
      481 tatgatgctg atggagattt ttgcttttcc tattctcctt tagataaaag cattgttttt
      541 aatgcctctt tgcttggcag caggttgctt agtaaaatgt attctttaac aaaaaatgag
      601 ctttataaaa tcgaagcaaa aaaatcggtt agttactgtg taaaacatca acaaagtaat
      661 ggtgcatggg cttatggctt acaaagtttt cattattgga tagataattt tcatacaggg
      721 tttaatttag aatgtattgc tgcctatcaa aaatacacag gagatacaag ttttgaaact
      781 gctatcagta aaggctttga atattatatt aataatttct tcgaggagga tggcaaacca
      841 aaatattata ataacaaaac ctatcctata gatattcatg cacctgcaca acttgtaata
      901 actttatatg aattagggtt aattcaaaaa gaagaaaaat tatgtagtac agtattaaac
      961 tggactctac aaaatatgca ggataataat ggatactttt attatcaaat taaccaatca
     1021 ttaaattcaa aaatacctta tatacgctgg gctcaagctt ggatgttttt tgctttagca
     1081 acatataaaa aagcctttca tgagaataga attgtttaac tgcccaatag atgctataac
     1141 catgcatcaa acacttaata aaattgaaaa tgcaattatt caaaaacagc atttacatca
     1201 tgtagtggtt aatgtggcta aattagtaca catgcaaaat aatagtgagt taagagaaag
     1261 tgtagttaat tgcgatataa taaatgccga tggacaaggt gttgtttggg cttcaaaaat
     1321 tttaggaaaa aaaattccag aacgtgtggc aggtatagat ttaatgcaaa atttggtaca
     1381 acttgctcat gataaaaagt ataaaatatt tttctttggg gcagaagaac atattgtaaa
     1441 agaggtagta aataaatata caaaccaatt ttcgccttct ataattgctg gctatagaaa
     1501 cgggtatttt aataaagaag aagaaagcga aatagcaaat gaaatagcaa attctggtgc
     1561 aaatatttta tttgttgcaa tggggtctcc taaaaaagaa aattttttat tcaactacaa
     1621 acaagtatta caaaaattaa attttgtaat gggtgttggt ggtagttttg atgtagtggc
     1681 aggcaaagta aaacgtgccc cagtttgggt acaaaaaatt ggtatggaat ggttttacag
     1741 aatggcacaa gagccacgca ggctttttat gagatatttt actactaact tagcattaat
     1801 taaattaatt ttaaaagaaa aattaaaaaa aggctagttc aattatgcac cataagtatg
     1861 cagcttttat aaggttttta aacttcattt acgatttagc aatattaaat ataatactgc
     1921 tttttttgtt tttaagattc agcccatctg tcattaatgt atctgattta ttgtttaata
     1981 aagtatattt ttttaattgg cttaatatca actttgcatg gattgctgca acaattttta
     2041 cgaatgttta tactttaaga aatttaattt attttaaaga aggagtaaaa aattctttta
     2101 atgggttagc tatttttatt ttgtttgttg tttgtattaa tttcttttta tcctctgttg
     2161 ttttcttttc taaaaaagat ttgctcattt ttattatact ttttagcacc ttcttttggt
     2221 tggctaggtt ggtattttat tattttaaaa aaagatataa tttagaattg tatgcaggta
     2281 aaagaatatt gctaatagga gatgaaagtg ctatagatga agttaaaaaa acattttacg
     2341 aaaaaaaaga attaggcttt gtatatgcag gacacttgcc agattctgaa tttcatgcaa
     2401 ataatcaaaa gtatattgaa aatcttttgg cctctataca actacaaaat atagatgaag
     2461 tgttttttgc aaaatcttcc atcaaagatg aagagttgta caatctcatt caaacattag
     2521 ataagaacac aataagagtt agagtaattc ccgatttttt tagattttat acaaagttta
     2581 aaaacctaag ttttcatggc aatctgccac tgttatcttt aagagaggag ccattacaaa
     2641 atatattaaa tagattacta aagcggtttt ttgactttat attttctcta ttggttataa
     2701 ttcttttagc aagctggcta ttcccattat tagcattatt aataaaatta gaatcgaggg
     2761 ggcccgtatt ttttaaacaa ctacgatccg gaaaagataa taaacaattt tggtgttaca
     2821 agtttaggtc tatgagagta aatgtaaatg cagatgtaat acaagctaca aaaggagatc
     2881 agagaattac aagaattggc aaatttttac gaaaaacaag tattgatgaa ttgccgcagt
     2941 ttttaaatgt atttattggc aacatgagtg tggttggtcc aagacctcat atgttaaagc
     3001 atacagagca atatgctgct atggttgata aatacatgat tcgtcatttt gtaaaaccag
     3061 gcattacggg ctgggctcaa gtacatggtt atagaggcga aatttctaat gttgaagata
     3121 ttgaaaaaag agtagagtat gatatttggt atatagaaaa ttggtcgctt gcattagata
     3181 ttcaagtagt attattaact gtttataata taataagagg tgaagaaaat gctcactaaa
     3241 acatactttt taaaatacgt tctacttgca attttggtta tttgtaagtt taatgtagca
     3301 attggtcaag ccaagtttcc tgttaaaatt cagaataaac tatatgcaca gttgtacgaa
     3361 aaatgggcta gagaaaacaa tatttatagt atagctgcat tacaaccata ttgcaataca
     3421 gatattagat ttaataaaga gctcttaagt ttttatcaat taccatcaga ttcattaagg
     3481 aaaaaaagaa atttttttac gcaaacattt agaaatggaa atatggcaga atttgtttct
     3541 gataaaagta cggtaacaat taatccttta tttcaatttg aatttggtaa atcaaaagac
     3601 tatagtgttt accattttaa tagaggcatt caactagatg caaactttaa taacaaagtt
     3661 tttatttcaa gcagcattta cgaaaatgtt tctaaatttc cgaagtatat taatcaatat
     3721 atagattcct taacagttgt gccaggcatg ggtaaagctc gctacaatac aaatggaaaa
     3781 aatattgagt atgcacttcc aataggtttc ataggatata aaccgaacaa aaatttttat
     3841 tttgaattag gaaataacaa aaactttatt ggcaatggtt atcgttcttt attattaagc
     3901 gatgtagcgt atagttatcc ttattttaaa atgcaaacac atataagcaa atttactttt
     3961 caaactatat ggagccagtt tgtagatgct tctaaaaatt gggttaatac aaatggttac
     4021 gataaaaaat ttggggcttt taatacagtt agttataccg gaataaaaaa tgtagaatta
     4081 aatttatttc aatctgttat ttggagtaat aaagatagct taggaaatag aagagatcaa
     4141 gaatggggtt attttgttcc aattattttc tttaactcat taaactttaa caacggctct
     4201 ccagataatt ctttaatagg ggtagatgct agttatacta taaaaaaaca tacagtaatt
     4261 tatggtcaat taataataga tgattttaat attagagaat caaagaatgg taaaggtttt
     4321 tttcaaaata aatatggtgt acagcttggt gttaaatctt ttaagccatt taatttagat
     4381 aatgcttttg caagaattga gtttaatact gttcgtcctt atacttatgc tagtaaaaca
     4441 ccaaccttaa gctatacaca ttatggcgag gttttagctc acccatttgg tgctaatttt
     4501 agagagtttt tactagaggc aaattataaa atcaaaagat acttatttag tgctaactta
     4561 attattgcat ggtatggggc agatgatgca aattcacatt ggggtaaaaa tatttataaa
     4621 tcagattatc tatcacaaac aggaattttt tcattcaata ataaaataac acaaggtatt
     4681 aaaaccaatt taatttattc taatgtggct ataaggtatt taatgaatcc tgttacaaat
     4741 tcaggcattg cagcacaaat attttataga acagagaaaa gtaatttaat aaataacaaa
     4801 gattttattt tcaatatttc ctactctact aatttactta atattctaaa agagttttaa
     4861 tttttagaaa agtgatcaaa attaatttta cgcattatcc ataaaaaaat aaaaggcaat
     4921 ggactagata aaaataatct tactcctgca aaaaattctc tccaattctc actcaaaaac
     4981 ttaaatttcc attcaagtac ccctattagc ccaatggtaa ttataacaaa agcataaata
     5041 attataacta ttttgctaag ccattgatta ttgtctttcg caaaagccca ccaagtaata
     5101 attcccacaa ttaataacca aattatcctt ttaagttgtt gctcaaaagg cttatgtacg
     5161 ttagatattt tatttatttc ttctgtattt gtgtaacccc accaacaaac aattaatatt
     5221 aaagaaatag caataatatg tttaaatgtt ttattcatct tttaatttgg gttttgtata
     5281 ttttacccac aataaaaaca taacagcata aataaaaatt ttaaaaaaat aatgatgggc
     5341 aacatctgta aaggaagagt tgtgaatatt taataatgta attacataac tacgtatatt
     5401 gtttaaaaca aaaattaata ctatgccagt aatagcataa gcaaataatc tttttttatt
     5461 agaagggaag caagctaaaa agccaatata taaaatatac aactcaagcc cattgcaacc
     5521 atctgcaata cctacaattt ttttattatt ttttaataaa ttttctttcg gtgaaggata
     5581 gtttatttct tcaatattca atttatcatt agggtaatga aattgtatca tttctttagt
     5641 aataactgct gtaatatgtg taagctgttt atctggaaca cgaataggat ataaaaccaa
     5701 atggtaaatc agtttccata taataaaaaa tactatgctt cttagtaaaa atttttttac
     5761 tgcagaagga atagtttgaa aaaaactatt agccataagc tttaaagttt aatcttaaaa
     5821 gtattttgaa ctaaagtgaa aataatatta ttaatccaat atttttacgg ctgtttaaca
     5881 aacaacagtg agtaaaaata atttttcttt cggctttaat caaaattcat ttaccaatga
     5941 acagttgcaa tataaggcat tgcaaataaa tggatcattg gctaaaagat tttttgatat
     6001 tgcagtttct atttgtttcc tcctctttat tttttcatgg ttatacccaa tcattgcaat
     6061 tgctataaaa ctagaaacca aagggcttgc tatgtttgtg caagatcgta ttggtttgaa
     6121 tggtaaaatt tttaaatgct acaagtttag aacgctttta agcaatagca attcgctaga
     6181 taaagattca ttgccactta gctatacaac acctgcaacc aaagtgggag tttttttaag
     6241 aaaatataat ttagatgaat tacctcagtt tataaatgtt cttaaaggca atatgtctat
     6301 cattggtcca aggccacatg cagtgccttt tcataattta tatagcgagt atgtgccaaa
     6361 tattcatcag cgtttattag ttaaacctgg tattacaggg ctttctcaaa taaatggatt
     6421 acgtggcgat gtagaagata tagagcagaa taaaaaaaat attcaacaaa gagtacaatg
     6481 cgatacaaat tatatctttc actggtcctt taaactagat cttaaaattt ttgtcaaaac
     6541 atttattggc taatagtatg cagattaaca ttattaataa atcaaccaac cctctaccta
     6601 cttatgaaac aaaaggggca tctggtatgg atgtaagagc aaatataaac gaagctatta
     6661 cattacaacc tttacaaagg gcattgatac caactggcat ttttattgaa atacctcaag
     6721 gctatgaagc acaaattaga ccaagaagtg gtttggctat aaaacaagga attacttgct
     6781 taaatacacc tggtactata gatgctgatt acagaggcga aataaaaatt attttaatca
     6841 atctttccaa tgaaacagta accatacaac atggtgaacg tatagcacaa atggtatttt
     6901 gtaaaattga acaggcagaa ctggtacagg ttcaaataat taacgaaact acaagaggta
     6961 caggaggctt tggacataca ggaaaacaat aaatttgcac aatgaataaa cagttattag
     7021 taggctgcac tttattatta gtagtaatat tctcttgccg tacaaccaaa aatactcata
     7081 atattcaaac agcaattact aaaaaagata ctactcaagt tgtaactgtt gccgaagaaa
     7141 aatcaataga ttcctttgct gttgttaaag aaattttgaa tcaaactata aatcataaaa
     7201 tcaacttctc taccttttat gctaaaataa aagtagatta tgaaagccct tcagatagca
     7261 gaaagcttac agcctatacg tatatgcaaa aagatagtgt aatttacata agattagtag
     7321 gtagcttttt gggaattact aaagaaggtg tggtagcaaa aattacaaaa gatagcgtta
     7381 ccgtaatcaa taaaattgat aaaattgttc aaaaaagaac tattgcttat ttgcaagaag
     7441 ttacacaaat accttttgat tttagcacaa ttcaagatgt cataattggt aatcctgttt
     7501 atatagatag caatatagtt tcttacagaa atagtttaga tgatttttct gtacttatgt
     7561 tgggcaactt gtttaagcat ttaattactt tagacaaaaa cgattttaaa gtattgcata
     7621 gtaagttaga tgatatagat gtttttaaaa acagaacttg taatattact tacagcgatt
     7681 atgtagatgc ttttggagta aagttttcta caaaaagaaa attttcagtt tcagaaaaaa
     7741 ctaaattaga tgtttggtta gattttaaac aatacacctt taacgagcct ttaacgtata
     7801 tgtttaatat tcctaaaaat tataaaaagc aataaaagtt tctccacaat tccattagtt
     7861 ttactgcatt gatagtaaac ttttgcacat agggcataaa taatattttt tttgtttact
     7921 tatgaccttt caatactata tagctattat gctttttatg taatatctat tcttttggta
     7981 tcttaaaaag aaaatcttgt atgaaaaaca aaccaagttt tattgtatta accaaaatat
     8041 cacctaaagc attatttaat gctgcaaaat tttttgtagc tattgctttt gtattagttg
     8101 cttcttttca acttcatgca caaacaaaag aagaactaca aaagaaagaa gatgagttaa
     8161 gaaaagagat taatgaatta tctaagaatt tagttcaaat tcaaaaaaat aaaaaagttt
     8221 cattagcaca agttgcatca gtacaaagaa aaattgcagc tagagaagag ttgataagaa
     8281 caataaatag aagggtgagg cttttagatg aaaccattta ccaaaatgag ttagaaattt
     8341 atagaatgcg taaagagctt gacaccttaa aacagcaata tgcccgaagt attgcttttg
     8401 cttataagaa tagaggaaat tatcaatatt taaatttttt attttcttcc accagtttta
     8461 acgatgctct taaaagagta acttacttaa aaagctatag aaaattaaga gaaacacaag
     8521 ttggcaacat cgtaaaaaca caacaggttt tacaacaaaa aattggcgcc ttaaccaact
     8581 ctaaaaacga gcaaaatcaa gtattagaat cgcaaaaaac gcagttagta gatttagaag
     8641 cagataaaaa agagaagaat gatgtagtaa aaggattaaa aggccaagaa aaagaggtaa
     8701 cagctttact aagaaaaaaa gaaaagcaaa gagcagagat gaggaacacc attgcagcaa
     8761 taattagaag agaaattgca gaggcagaaa aagcaaggtt ggcaaagtta aaagcagaaa
     8821 cagatgcaag aaataaagca aatgcagcca ataaaaatat tgcaaaaact acaactacaa
     8881 aaccaggtgt tgcaaacaca gaagtagtgg aagaaatggc aagcaccaaa acggtaaata
     8941 gagagtatag tgcattagaa agtactgcag aaggtgtaga aatgtcggtt aattttgaaa
     9001 aaaggaattt gaattggcct gtaagtattg gtaccgttat tgcaccattt ggcaattatc
     9061 aaataacaga aaaattacaa ggccatagcg atggaattga aatagctgtt ccacaaggta
     9121 ctgctgtaaa atctgttgca gatggtgaag taacctatgt aggagatttt ggaggctcgc
     9181 ctgctgtaat tgtaaagcat ggaaaatatt ttacaattta taattatttg tcagaaatta
     9241 cagcaagaaa gagccaacag gtaaaggcag gtacagtttt agggaaatcg ggtataagca
     9301 cttttggtga agaatcttta ctttttatgg ttaccaacga taaaactgtt tttttaaacc
     9361 caagaaattg gttaaaaagc agataatttt tctttatact actctattaa taacaggtat
     9421 tttatcctat cactatttta gcttaactta gtgcacattc gtaactatgg aagaagtgta
     9481 caatttgatt tggctttttt gttcaattat tataattggc ttttttgctg gttacgaaat
     9541 tgcttttgtt agcaccaata aattatctgt tgaacttaag aaaaaacagg gcaaaaaaag
     9601 cgggatcgtc atagcaaaat ttttagaaaa acccgctgaa ttcattggct cttgcttaat
     9661 tggcttaaac tttttttacg ttatttatgg gcttgctttt aaccaatttt tacaaggctc
     9721 tttttggagt ttctttcatg ttaaaaatgg gtatcttcaa cttgcaataa acactttaat
     9781 tgctggtata gttattttag ttttaggtga gttttttcca aaagcaattt ttagagccaa
     9841 aaatgatatg ctgattagtg tttttgcacc cttagctaat atttttcata gcttgtttgc
     9901 acccattgca aggttttttg taagtgtttc tcaatgggtt ttaaaatatt tgtttaatat
     9961 taaaatgagt gaaaaagaag aagctttctc tcatattgat ttagaacatt tttttcaaca
    10021 agccaaagaa catgatgaat ctggcgaaga attaaacaaa gagttatttg aaaatgcact
    10081 tagtttacct acagttaaaa taaggcaatg cttagttcca agaactgaaa tagaagggat
    10141 agacatagaa agtaatatag aagaagtaaa gaaaattttt atacaaacaa aactaaccag
    10201 gctaattgta tttaaagata atattgataa tattttgggc tatattcatc agttagattt
    10261 atttaaaaaa cctaaaaata ttcaagatgt attattgcca attcctgtta taccagaagc
    10321 tatgaatgca atagatttaa ttagcaagtt tacaaaagaa agaagaaata ttgcatgggt
    10381 ggtagatgag tttggaggaa ctgctggtat tgtaactctg gaagatattt tagaagaaat
    10441 ttttggcgaa atacatgatg aatatgatac agatgatttt gtggctaagc aactatcaga
    10501 aaatgaattt gttttaagtg gaaggctaga gcttgatatg cttgcagaaa aatataaatt
    10561 aacttttgat gtagatgact cagaaacctt atctgggtat atcattaatt tttatggctc
    10621 aattcctaat caaagagaat caataattat tggtaattat cagttcgata ttttaagtgt
    10681 tagcgatacc agaattgaaa atgttaaact gaaagtatta taattatacc cttacttttt
    10741 tactcaatac tttattaaaa agaatatata aatctaatac aataataaac cccacaaaaa
    10801 gcgttatata taatttgaat tgaaagtata atatgctcta atgcctgtta cggaatctat
    10861 agaaaccaaa aataatcaat tgcttgctca gtgttacgag caacttatta ataacaatat
    10921 taatgatgca ttaagtaata ttacaaacct tataattcag tacactaatg cagcaatgtg
    10981 ttgtattagt gtaaatagag ataaagcaaa caaattgtat cattgtgcta ataatgacca
    11041 aataattttc tctgaaaata aagtacaaca atttttcaaa cagtttaaag aaattcaacc
    11101 aactcattcc tcttttaact tacgttcaaa tagtaatgaa aatatttctt tgtttgccaa
    11161 ttctccaatt aagcaaattt taggctacag ctttaaccat aataacaatg aactaatatt
    11221 attggctttt aaggaacctg tagaggaaaa tatatattct tctattttac aaaaattatc
    11281 aacagcaata agtaaagttg caagcacaac aaccattcaa gaagaattgt tttttagaaa
    11341 tgatgtgtat gaatctactt tatatacatt aaaccaaata atttgggatc ataatttaaa
    11401 aacagatact acacatgtaa tggggtttgc ccatcgtata ggcaatttta tacctcaaaa
    11461 aacatttcag tacaatataa aagattggct attagataaa gcacatccag atgatatgca
    11521 aagggtgatg cagaattatg aacaatttat tcttcaaaaa aacaatactg ttaatgaaat
    11581 taaatttaga atgtttagtc caactgccaa tgaatatttt tgggtgcaga cgaggcaaac
    11641 actgctgtat gatgaaactg gtgagcctgc attagtagtt ggtaccacaa tagatattca
    11701 agatacttac caaatagctg cagatttaga aaaacataaa atccaaaatc ttctgctggt
    11761 agaatctttg caagaatcca acgaaaaatt attaaccatt ttaaatagta gtaaagaaat
    11821 aatttttact attgatttgc aaacaagcca atttgaaaat gtaaacaaag caatcagttt
    11881 acttggctac acacccaaag aatggattgg tcaaaactac acacaatggt ctttagataa
    11941 aagaaaaaaa tttcataact tattaaagca tgccagtgca agcaataatt cagttaaaac
    12001 acagcaaatt agttttacaa caaaagatga gaaaacagaa attcctttcg agttttctac
    12061 ctctgttttt actttcaaaa ataaaaaata cttactctgt attttaagag atatctctga
    12121 aagagtagca tacgaaaata gtataaaaca agtaaccgaa cagcttgcat accttattaa
    12181 taatatagat gatgtgtatg ctatttataa tttacaacaa aaaaaatatg aatttgtaag
    12241 caataatatt gaatatttat atggctgtag taaagatact tatctctcca acgatttata
    12301 ctggaaagaa attattttta aggatgatta tgccagtata gaaagccaaa tgaacgatat
    12361 aattagcaat aaaagtaaag gcgaattttt ttatagaatt aatacaccag ttggagaaac
    12421 aaaaatgttg ttagaaaagg taactgtaac aacagataaa gatggcaatg ccgataaatt
    12481 gtttattgta aaatctgatt ataccaattt agaaaatgcg gaacaaaatt taattgaaag
    12541 tgaacgtaag tttagattta tttcagaaaa catcactgat tttatttcta taattgatat
    12601 tgatggtagg tttatgtatg catctccatc ttctgtaaaa gttataggct acactacaga
    12661 agagttaatt ggaaaaaata ttattttctt tattcatgcc gaagatgtta ccaacttttt
    12721 agaagaagct ttagaaaaaa tagtatttaa taaagaagag gcaagtttaa gatataggtt
    12781 aaataaaaaa gatggcagtt ttaattgggt agaaacttat ttcaaaccaa ttatagatgc
    12841 aaacaacgat actacatcta taatttgcag cactagagat gttactgaaa gagaacagtt
    12901 aatgaaagag ttgcaacaat ctttaaataa agagagagca ttaaacgaat taagggctca
    12961 gtttgtttca atggcatctc accaatttcg tacgccacta acagtaattc aaagcggtat
    13021 tgaattaatg caaatttatt tagaaggcat ttctgaacaa aaacaacagc catttaaaaa
    13081 acaattttta agaatagaat ctgaagtttc aagactacaa gatttaatga atgacgtatt
    13141 acttttaggc agggcagagg ccaatagaac accttataac cctacttttg gtaacatcat
    13201 tcaattttgt agagacataa tagaaagtag gtataataac caggattata acaacagaaa
    13261 agtaatatta tatattttcg gaactgctca agaagtaatg tttgatgcta aattattaga
    13321 ccatacttta gaaaatattt taagcaatgc tttcaaatat tctaacaagg gagatataaa
    13381 atttactatt acttttagtg aaacaacagt aaatattgct gtaaaagacc aaggcattgg
    13441 catacctgca aacgaaattg aaaatttatt tcaacctttt tatcgtgcca caaatgcaac
    13501 agaatttgaa ggtacaggtt taggcctatc cattgtaaat gaatttatag aaacacatgg
    13561 aggtaaattg tttgtggaaa gtgaaataga taaaggcacc actgtaaata ttcaacttcc
    13621 attatatcaa ataaaaaaat aaaaccaata ttttatgagt tttacaacta aaattttagt
    13681 gatagaagat gaagataata taagagaaaa tattcaagaa ttgttagaag ctaaacaata
    13741 tcttgtacgc acagcaaata acggaagaca gggcgtttta gaagcaatag attttaggcc
    13801 acacttaatt ttatgcgata taatgatgcc taaaatggat ggttataaag tgttagagta
    13861 cattcgtaaa actagtatta ttcaaaatgt tccatttata tttttaactg ctaaaattga
    13921 tagaaatgat ataaggcaag gaatggaatt aggagcagat gattacctaa ccaaaccatt
    13981 tactgctaaa gatttagtaa cagcaattga aacaagatta aaaaaacaac aaaaaataat
    14041 tgatgaatat gcaaaagtaa aacacgaatt agatacctct gcttttgcaa gctattatca
    14101 tgagtttaat acaccattac atggcataat tggaggcatt aatttattat taagttccag
    14161 aacttctttt aacgatactc aaattcaaga actactttat tcaattttaa aatctgggtt
    14221 aaggctaaac cattcattag ccaacctaat gctttttgaa gaaataaaaa gagcagaagt
    14281 gcatactgaa ttgctaagca tgtttaactc tggtaattcg tcaggtaaat gggcagatag
    14341 aattaaaaca gaactagaag ttattgcaag agaaatttat aatagaccag aagatttagt
    14401 ttttgatatg caagccaccg atgtgcatat tagtcctgaa tatttaatga gaattttatt
    14461 agaactaaca gataatgcat taaaattttc atcaaaggga agcaaaattt ctataaaagg
    14521 aaaaccagat ggagataatt attttattga aataaaagac agtggtaaag ggtttgctat
    14581 tgaaaataac aatcaaatag caccttttaa gcaattcaat cgttctaaat atgaacaaca
    14641 aggcttaggc attggcttgt atttggttaa aagattagtt gaaattaata gtggagaaat
    14701 taaaatagaa tcagaagaaa ataaaggaac cactataact attttattgc caagttttgg
    14761 tatttaactt agctgtaact ttcgccttta tctttgtttt atgtataagc taattcgtga
    14821 tattttgttt tgttttcctc cggaaaatgt gcattatttt tcaatgaata tgctaaaaat
    14881 gctttgttct aacaaggcaa ttaaaaactt aatcagtaac caatttcaat ttcaacaaac
    14941 aaccttacaa aaagaagttt ttgggttgca atttaaaaat cctgttggct taggtgcagg
    15001 tttcgataaa aatgcacttt acttaaacga attaaatgca cttggttttg gatttgtaga
    15061 aattggtacc gtaacaccat tagctcaaaa tggcaacgat cggccacgtt tatttcgttt
    15121 gcccaaagac aaggctttaa taaatagaat gggttttaat aatgatggtg ttcatgaaat
    15181 aaaaaaaaga ttaattcatt ggcgtaacca gcctagcctt caaagtaacc aattaattat
    15241 tggcggaaat attggtaaaa ataaaattac acccaacgaa aatgcttgga aagattatga
    15301 aatttgtttc aatgaattat ttgaattggt tgattacttt gtagttaatg taagtagccc
    15361 aaacacacca ggtttaagag aattgcaaga aaaagatgct ctaagaaaaa ttttggcaca
    15421 tattcaaaat caaaatcaag gcaaaacaat gcctaagcca ttactcttaa aaattgcacc
    15481 agatttaacc caagaacaac tagatgatat tattgattta ggtatagaaa ttaagttaga
    15541 tggattagta gcaaccaata caaccattga taggcaaggg ttgcaaacaa atgctcaaaa
    15601 aattcagcaa attggtgcag gtggtttaag tggcaaacca gttgcattaa aaagcactca
    15661 agttgtaaaa tatttggcac aacaaacaca aaatcaaatt cccataattg caagtggtgg
    15721 tatttttaca gcacaagatg ctaaagaaaa attacaagct ggggcttccc taattcaagt
    15781 ttttacaggc tttatatatc aaggaccttc aattgtaaaa aatatttgta aagagttgta
    15841 actatcaaat ggttaactgc tatggtctgc aataattacc catttgcctt taatctttct
    15901 aaataataaa ctgtaatgcc ctgaaatatt gccaacagtt ctaaaaagct cccatttgcc
    15961 tatcacaaaa taatattcat ttgaaagttt tttaatgcta ataatagtag aagaaaattt
    16021 tcccatatgt gctgtatccg gataactttt tttataattt gctaaagtag catggtagcc
    16081 ataagttaaa ccctttttac caataaacat aaggctatca ttattccagt aaccaaacat
    16141 aaaagcatct aaattaccat tgttccaggc tttctcttgt gtttgtaata tttgcctaat
    16201 agcagcttca tcggtagttt gagcatttgt tttaattgta aggataatta aaataaaaaa
    16261 tattattctt ttcattattg gtattgtttt aaatgttttt aaagataata aaacgctact
    16321 ttgatataaa ataatttctt tttattattc agcaataatt aggtaaaata taaccttatt
    16381 acttaacttc gccaccttta aaataaggca ttacaagcat tttgggcaat gaaaaaatat
    16441 aaagaatttg aagggttaaa cctcccggtt atagaaaaag aaatattaca acagtggcag
    16501 caagaaaaag cttttgaaaa aagcatttct ttaagagaag gctctacacc ttttgtgttt
    16561 tacgaaggtc caccaagtgc caatggtatg ccaggcattc atcatgttat ttcaagaaca
    16621 ttaaaagatt tggtttgtcg ctataaaacc atgcaaggtt ttcaggtaaa acgtaaaggt
    16681 ggttgggata cgcatggttt acctgtagaa ttaggggtag aaaaagaatt aggaataact
    16741 aaagaagata taggcaaaaa aatttcggtg gcagagtata accaaaaatg ccgtgaagct
    16801 gttttgcgtt ataaaaaaga atgggatgat attactacca aaatgggcta ctgggttgat
    16861 ttgaaagagc cttatgtaac tttcgaaaat aattatatag aaagcctatg gtggctatta
    16921 aaaaagttat acaatagagg actgttgtac gagagtgtaa gcatacagcc atacagccct
    16981 gctgctggta caggtttatc ttctcacgaa ttaaaccaac ctggtacata taaagatgta
    17041 aaagatacca gtgcaactat tttatttaaa gtaaaagaaa acgaaaaaaa taaatttcaa
    17101 aatattgaag gcaatatttt tttcatggct tggactacaa caccatggac acttccttca
    17161 aatttagggc ttactgttgg tgccaatatt gattatgttt tagtaaaaac gttcaatcca
    17221 tttacccata aaattattca tgtaataata gcaaaagctt tacttggtaa gtattttaag
    17281 gcagaagatg aaaatggtga ttttgaaaat tatcaaccag aaaataaaaa acttccatgg
    17341 caaattatag ccacatttaa aggtagcgaa ttagaaaatg tggaatacga acaactatta
    17401 cctttctctg ctaatacttt aagtgtaatt aaagaacaaa cacctaatgc aaaacctttc
    17461 agagttttat taggagattt tgttacaacc gaagatggta caggaattgt tcacacagca
    17521 cctgcatttg gtgcagacga ctttagagta ggaaaaaaat acgatatagg tattttaacc
    17581 atggtagata aaagaggtaa atttttaacg ggtattgaag atggcatttt tttgtatgga
    17641 gaggaatatg ttaaagaagc atatttaaca gaggcagaaa aagaaacaga atttaaaaaa
    17701 caaaaacaaa tattagaaac caacgaaaaa gtaaaagact taaaaaaata tttaagtgta
    17761 gatgatagaa ttgtattgaa attacaagaa gaaggaaaac tatttaaaaa agaaaagtac
    17821 gatcataact atcctcattg ttggcgtaca gataaaccca ttttatatta tccattagat
    17881 gcatggttta taaaaactac tgcattaaaa gatagaatgg ttgaactaaa caaaactatc
    17941 aactggaaac caaaaagcac aggagaagga cgctttggaa actggttaga aaatatggtg
    18001 gactggaatt tatctcgttc aagatattgg ggaactcctt tacctgtttg gtgtaccgaa
    18061 gatggagatg agcaaatatg tataggttct atagcagagt tagaagccaa tttacatcct
    18121 aatagcccag taaaaaatat tacagattta cataagccac aggtagatga agtaattcta
    18181 caatcttcta gaggtaaaga aatgaaacgt gttccagatc ttgtagatgt gtggtttgat
    18241 agtggtgcaa tgccttatgc acaatggcac tttccttttg aaaatgtaaa aacattcacc
    18301 gaaaattacc ctgcagattt tattgcagaa ggtgttgacc aaacacgtgg ctggttttat
    18361 acattgcatg ctattggttc tttaataaaa gaagatatta ttgaagaatt aaaaaatcaa
    18421 ggatacagtg aaaatactat tgagcaagta aaaggtgtag catataaaac cgttgtaagc
    18481 aatgggttag ttttagataa gaatggaaat aaaatgagca aacgcttagg caacgttgta
    18541 aacccatttg atactattga aaaatttgga gctgatgcta ccagatggta tttaataacc
    18601 aatgcaagcc cttgggataa tttaaaattt gatattgaag gaataaaaga agtgcaaaga
    18661 aaattctttg gaacccttta taatacttac caattttttg ttttatatgc caatgtggat
    18721 ggatttgctt ttgaagaaaa atatattcca ttagcagata gaccagaaat tgacagatgg
    18781 attttatcct cattaaacac tttaattgaa aagtgtacta gtgccatgaa cgattatgaa
    18841 ccaacagctt caggcaggtt gatagaagag tttacagatg aacatttaag taactggtat
    18901 gtacgtttat ccagaagaag attttggaaa ggagaatatg aacacgataa aatttgtgct
    18961 tatcaaacct tatacgaatg tttagaaact attatgcaat taatagcacc aatttcccca
    19021 ttctttagcg atgctgtttt tcaaaactta aatgctgtaa caaaacggtt taatgcttca
    19081 tcaattcacc atgtttccta cccaaccagc aatgcaaatg caattgataa agatttagaa
    19141 gaaagaatgc aattagctca aaatacttgt tcattagttt tatccattcg taaaaaatct
    19201 aacataaaag ttcgccaacc tctacaaaaa ataatgatac ctgtattaaa tccaacaatg
    19261 aaaatacagt tagaaaaagt agaagaccta attaaaagcg aagtaaatat taaagatatt
    19321 caatacttaa ctgcaacaga aggtgtaata aagaaaaaag caaaagcaaa ctttaaaaca
    19381 ttggggacaa agttaggtgc caatatgaag gatgctgcta atataatttc aaattttagc
    19441 caacaacaaa ttgcagagtt agagaaaagt ggaagcttaa atattcaact ttcaaactca
    19501 gaataccaac ttacagtatc agatgtagaa atagtagcag aagatatacc tggttggtca
    19561 gttgcatcca attcaagtgt aactgttgca ttagatataa caattacaga agccctgcaa
    19621 agtgaaggag atgcaagaga gtttgtaaat cgtgtacaaa atataagaaa agaaagcaat
    19681 tttgaactta cagaccgtat attc
//
LOCUS       DAOQHP010000004        18132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1428_length_18132_cov_3.929856, whole genome shotgun sequence.
ACCESSION   DAOQHP010000004 DAOQHP010000000
VERSION     DAOQHP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18132
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_18132_cov_3.929856"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..408
                     /locus_tag="PLY81_00330"
     CDS             52..408
                     /locus_tag="PLY81_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28704.1"
                     /translation="MEQEGNLVLLDQAAHLLHRLRRAVAIIVGDEIDLEKNTMQVNKI
                     NWIKYNGISDGMEALTKIRYKDKGAFSNLYNNENGIKVQFYEPVKSIAPGQSAVFYEG
                     DDVIGGGIIQSNIFNF"
     gene            487..1233
                     /locus_tag="PLY81_00335"
     CDS             487..1233
                     /locus_tag="PLY81_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28705.1"
                     /translation="MKLFVRYSVLIFSLFFVACSSKETINLPTVAELNPLQNGKVFIY
                     RLDSTVITNFSKSLAIKSYHAKDSIADNFVDVSGKQSFKIYRYITDTLQTNPWIYNAT
                     FFVSFNKNNIEFTDNNNLRTITIASPIKEGTQWYGSKYINAPNTSADYYFLKDWFFEI
                     KNADLPFTVKKGTINNTYTILQADEADPGGPLNPSLPRSYRAYSVEVYAKGIGLIYKE
                     FLHWEWQNPNNDGAYTNNSYGIKLNLIDYK"
     gene            1258..3246
                     /locus_tag="PLY81_00340"
     CDS             1258..3246
                     /locus_tag="PLY81_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF28706.1"
                     /translation="MKKIIIAAFFLINYVSAEAQFSRYTIQLKNKLFNSYSTNNPSAF
                     LSQKAIDRRVKYNIAIDSTDLPITQKYIDSIKLAGNVTILNKSKWLNQITILTTDASA
                     INKITNFYFVESVNNIAAKPLKENKKDTLQKNKKIQFKNNITISYTPYSKEQALNNYY
                     NYGLTLNEIKLNNGQFLHNIGMRGQNMLIGVLDAGFYKYNSLPAMDSMNLNNRVIETW
                     DFVNNETSVVEDNSHGMSCLSTIVGNIPNNFIGNSPEASVLLYRSEEGVTEYPIEEFN
                     WVCALERADSAGALVITTSLGYTTFDNASFDHTYSNMNGKTTTAAKGGTFAHRKGMLL
                     FTAMGNEGTNSWKYLSTPADLDSTISVGAVNTSGNIANFSSYGPTSDGRVKPEACTVG
                     WNAYVQNPNNTIGQGNGTSYACPKMAGLGTCLWQAFPELNNYAIRNAIIKSGSIFNNP
                     DDRKGYGIPDMKKAFAILLPQFSKITNYSNNDCAININWQSKDVGSMQYIVERRLTNE
                     SNYTVLKNINGSEDILAIHQYEFKDTVKTTLPSTVAVYRIKQVIDTTTAGYTTVNIDS
                     VSFNLNQCIASTEIISLLPNPAQNNCTIKIETPYNIPNLIIRILDIKGNIVSSQKFNK
                     PIGVSLFKLPIYFLAKGIYIVGVYNNDELIENKKLIKQ"
     gene            3299..3709
                     /locus_tag="PLY81_00345"
     CDS             3299..3709
                     /locus_tag="PLY81_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF28707.1"
                     /translation="MNLLTTEWKAGKEIGILILRLAAGYFILYGHGFNKLEVIFTGQE
                     IKFLDPIGLGTNLSFYLVAFAEGICALLIILGLYTRLAALVLAFNFIVILIVHSADNF
                     IDIEHIYLYLFSYLTLIFTGSGKLGLDNYLFNRK"
     gene            complement(3746..4942)
                     /locus_tag="PLY81_00350"
     CDS             complement(3746..4942)
                     /locus_tag="PLY81_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HNF28708.1"
                     /translation="MRFQSYLNTAVSLITNYYGNIPLVHYLKNYFAQNKKHGSKDRKA
                     ITHLCYCYFRLGKSLNEVTFEEKIKIALYLCHNKINEYGILYNNNWLANWHSSLAQRI
                     DFVQKNYPNFSIHSIFVFTDLLSEGICKEEFVLSFFNQPQLFLRVRPNYLQTVLQKLQ
                     AAAIAYKLLNNNCIALPNTTKVDAILQVNKDVVIQDYSSQQIAQYYEIVLSALNVNNY
                     STHKPLLVWDCCAASGGKSILLLDTFPNIKLTVSDIRESILINLAKRFKEAGINNYQS
                     FVTDLSKEQQDIKFNKYQFIVCDVPCSGSGTWGRTPEQIQFFTNEKLNNYTLLQQKIT
                     LQAAKHLNKNGFFLYITCSVFTTENEDIVKFLLANTALQLVQMQNIVGYNNNADTMFV
                     ALFKNV"
     gene            complement(5164..5490)
                     /locus_tag="PLY81_00355"
     CDS             complement(5164..5490)
                     /locus_tag="PLY81_00355"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNF28709.1"
                     /translation="MIHHSDRGIQYCSDKYQYVLEKQKNKCSMTKNSDPYENAVAERV
                     NDILKQEFMIDKLHLDLPLIKKVVSESIQIYNSVRPHLSNAMLTPNQMHFQLERKMKT
                     YKTKNR"
     gene            complement(6107..6229)
                     /locus_tag="PLY81_00360"
     CDS             complement(6107..6229)
                     /locus_tag="PLY81_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28710.1"
                     /translation="MSKTPEQRIMELEAKVKLLEKQKSLLEHQSHIADQKAIIF"
     gene            complement(6544..7263)
                     /locus_tag="PLY81_00365"
     CDS             complement(6544..7263)
                     /locus_tag="PLY81_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF28711.1"
                     /translation="MNIKQIALLYLLCSAFITTATAQKYKFEIGTEGGLVYTKYKIKD
                     PQKNLQNVPCQSGIGGINFRINTQKQKWFYEIAFLAMEYTDGLKLKQQNSWFTSNSDA
                     VIMFSLRAGYNFKLDKNFSIIPLIGLAPVIKTLNQDGGEGYYEEGNSSFNSLYHYTRI
                     EIDKNIFMLLQGGIGIEYRFAKKLKFSAAFNYYQGFTNNAIYDINYQINTDPVQTANM
                     YGKGTFVNYQAGLKFLFCRQK"
     gene            complement(7405..8637)
                     /locus_tag="PLY81_00370"
     CDS             complement(7405..8637)
                     /locus_tag="PLY81_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28712.1"
                     /translation="MKKYISLALIYAILITLSVSSCNKRLENNVSHEISGQTINASDI
                     PLLTDEVVAKIEEEKSHLEILKDEICLQVANIAQVVTPTGDGSLTYPNWVRFVEIAEA
                     IDSLNDNWNEAYGQALDNIINTTNIDPNDEAGQEAFSDMLEEDYLIFPADMASECFEN
                     MFSFESLRMCLETSEDDWLENSIEFNPANAPTIQEPIEDEEYWTLITCDRRYVISNTT
                     VDEDMMEGREDSKIKELLGVIQDIVGVLGGVVNIVREISSLLEDCAGSTTTTERDVRD
                     GITIPNETDKWIGYVMKQKGVLFDFNQTQTKIKGKAKIWKLNSKGKRKKDRKNKASID
                     FCVKEWNNCDGGDWVYKSPVSSKRKKVKIKFYQPYALVVGNPTHYLQFGFGRNGIYAT
                     SLNLLGTTGCRNSSNTDW"
     gene            8981..9355
                     /locus_tag="PLY81_00375"
     CDS             8981..9355
                     /locus_tag="PLY81_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF28713.1"
                     /translation="METIGQRIAQERFAKRWTQEKLASELGITQSLLSDIENDKVSPK
                     WQTILSTAEKLEVPFSKLLPNDSNILYQPSMNDNSIGFVHNYNSFADERKLWEALLQA
                     KDEIIQSQKLVIATLQEQGKKG"
     gene            9609..11594
                     /locus_tag="PLY81_00380"
     CDS             9609..11594
                     /locus_tag="PLY81_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF28714.1"
                     /translation="MKSIWIYLFVALGFIAAVFPPEQKKVALIIAIGDYSEAPGWRNL
                     SSMNDVRYVKQALLKVGFATNNIDTLINKDATKQGMIKALDDLYNKVGEGDIVYFQFS
                     GHGQQIQDDNGDELDGYDEALIPYDASAMYDPVTYSGDKHFRDDLLGEKLNKIRKKIG
                     PKGSLVVLVDACHSGTATRDAGIKRGMEKPFLTDPKYVPNIKINLNKEGEHGLLEGFT
                     NGMGNMIVFSASSPNQPNYEMKDNNNNGVGSLSYAFAKSIAELPAESSYRVLFHKMKA
                     IIQGDIPNQIPMVEGDLDLEVFGGKYIPKPTIIAVDQKFNNASNKFNDTTFVISIGQL
                     NSIYKGTTLKIYPVGSAEAYCDAVVTSVSSFQSICKSNKPLKKSVAYEAKVEAASAGD
                     FAAELMIQNNETNSKIANTVASSFSNFVKPYQYLSFSNNPDFTLDISKNNNGGYTVSL
                     LGKGDDVRYTKTITDSLSIEDCKYFLEGIKRNMRVKYLRNLPDGGTLAKDIKVEIVPK
                     KATNNQNEIVMSGKDLFEIRITNTGKSDYYYTIIDIMPENEMKVLLPDETSEPQDFVI
                     QPGQTIPIGDIEVDETTPNGKEIFKIIVSKIPMDLRNVVNRTRTRSANAAMKSVESVI
                     DDLFKDSNDQRATRSSISNVKVDEVGILSCGFTVKNK"
     gene            complement(11656..13332)
                     /locus_tag="PLY81_00385"
     CDS             complement(11656..13332)
                     /locus_tag="PLY81_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28715.1"
                     /translation="MKVVKKLFFTLSFIMPALLLAQSSYLTLGGKEEWLLNRMEIKTN
                     ANGLSFSSLKPFNRKYITNTADYWDSLYTAGNKNATVLTSTDKYNLQRFLMANSEWSK
                     PKEIFKSEKPIWKYFFHNRSNMIELQNSDFVLIANPILQYEQGSKGGNSKSTFINKRG
                     INVRGLIGTKIGFHFYFTENQERLGDYVAAWKNKFIAYPGAGYIKNFKTGGYDYFDIR
                     GGLSWKVAKFMDMQLAYDRNFIGNGYRSLFLSDFSTNYMYFKVYTHFGKFQFQNIFAE
                     LVSYKKSGSDRIYPRKYFRASYLNFKATKWLNLGLFEGLMLADNSAVSLPLFNPIMFT
                     NVSGNKNDKSYIGFDVKANIAKRFQLYGQLMIDKLKTDELKNDWWGNRFAYQAGLKYT
                     DAFGIKNLDVQVETNVARPFTYAANDSVTSYNHYNQPLAHPLGANFKEYIGIIRYQPI
                     KKLYVEAKAIYYKQGLDSSILNINQNYGSNIFNFMNTRPFENGWKIGTGNLANCTLIS
                     LVASYEIKENLFIDIAYLTRNFKLKTAGITNTNILSAGIRWNIGRREMLF"
     gene            complement(13359..15014)
                     /locus_tag="PLY81_00390"
     CDS             complement(13359..15014)
                     /locus_tag="PLY81_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28716.1"
                     /translation="MMQSIKTIVIILFVLFCYSVSAQTSYSLLGHKEYNILDRLEIKS
                     KLPAFGASFIKPYNIRLLVKEVETIDSLQRNNNRIVASLSTVDKYNITRFLMQHKEYT
                     SLTSSFNNKKSAFNLIEINKPKFFMTINPALQVSYGAETKDKQSLYSMRVGLNATGLV
                     AKKIAFNIYATGNRERTPLYVRAYTSKFKAVPGFSNFNFTDSNAVQYFDVRASAQWTL
                     AKFMDMQVGYDRNFIGNGIRSLFLSDFSGNSLFFKSNIRLWKLNFESLYMQLTPQFGI
                     TTKANFKKYLRINTLSIDATNWLNIGIFDAVVFGRKNQFELNYLLPFTFLRAMEQQSG
                     SPDNALMGLNVKANLKNKIQLYGQVLLDEFLLGEIKANRGWWANKFGYQLGVKYLDVL
                     NIKNLDMQVEINRARPFTYTHFDSVSNYSNNNMPLAHPLGAGFQEYIVATKYQPLKKL
                     FLDAKFIYYYQGQDTAGINFGNNILRDYKDRPTNYGWKIGSGNKATCIYFNTNIAYEV
                     KENLFFDAGIVFRNYKLASGTTNNTVIFNVGFRWNITKQQFEF"
     gene            15049..16392
                     /locus_tag="PLY81_00395"
     CDS             15049..16392
                     /locus_tag="PLY81_00395"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF28717.1"
                     /translation="MNHYLIKDTHIVNEGKIVSGDVLIKNGIIEKVGGVINTKNNPIE
                     VDGSNQHLLPGIIDDQVHFREPGLTHKATIYSESKAAVAGGVTSFMEMPNTSPPTFTQ
                     ALLEEKYAIAKQTSLANYSFFMGTGNDNYEEVMKTNEKKNEVCGIKIFMGSSTGNLLV
                     DNPILLDKIFANAELLIATHCEEESIIKNNLEKLIATKTNLEAADHAVIRNEEACFEC
                     SFKAIQLAHKHNTRLHILHISTQKELQLFGNIVPLKEKRITAEVCVHHLHFTANDYAI
                     LGNKIKCNPAIKAAYNKEALWQALLNDKLDIIATDHAPHTIEEKNGTYLKAHAGLPLV
                     QHSLMLMLHYVQQGKITLEKVVEKMCHSVADCFTIKNRGYIKEGYFADLVLVNCYQPY
                     TINKENILYKCTWSPLEGFTFPATITHTFVNGNVVYSNGVFKEEFKGSRILFDRK"
     gene            16418..17734
                     /locus_tag="PLY81_00400"
     CDS             16418..17734
                     /locus_tag="PLY81_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF28718.1"
                     /translation="MQTDKVTLQDLSIFNADESLSVLHYLNFTTTNRGKPFLYNILSH
                     PLSTIEEIEDTQTTIQYLNTLIPKWNIKITNGTLMVVEKFYETAFNHYPTNPSTINSF
                     FYKLTNNADFSLTKYSVTHCIYFLKGINQVLLFLSDAKSKKLLFYKEKLSLILNKTIV
                     QEIILIDDVKKIKNTSILKYAYFLKHEFKHNMQQLIVVFEQLDAYLSLAKACEKFQLT
                     FPKFIESDQPFIEAEGLFHILLPKPVSYTICLNNSENFLFLTGANMAGKSTFIKSVGI
                     AVYLAQLGLGVPAKKMTISLLDGMLSNIQISDNIINGESYFYNEVQRIKQTVEKISGN
                     KKWLVLIDELFKGTNVQDAMKCSSIVVEGLLKSSKTLFILSTHLYEIAEDLKKHSNIQ
                     FKFLETTLEKEQLVFNYQLRNGISEDRIGYVILKKEGVIEMLNKLN"
     gene            complement(17731..18024)
                     /locus_tag="PLY81_00405"
     CDS             complement(17731..18024)
                     /locus_tag="PLY81_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HNF28719.1"
                     /translation="MNLLFTFLSSQIELIGYIGSFLTSITFLPQVFQAWKTKSVGDLS
                     IWMISIVIISTIVWLVYGFGIKGGGPVIVANFIVLALSLVLLYFKLSFKKNKN"
BASE COUNT         6372 a         2876 c         2682 g         6202 t
ORIGIN      
        1 cttctggttt acatagcgca ggatcgcggt attcgtcggc gtgcccaggg tattgaacag
       61 gaaggcaacc ttgtcctcct cgatcaggcg gcgcacctgc tccaccgtct tcggcgggct
      121 gtagccatca tcgtaggaga tgaaattgat cttgaaaaaa acaccatgca agtaaacaaa
      181 ataaattgga taaaatacaa tggcatatca gatggcatgg aagctttaac caaaataaga
      241 tataaagata aaggtgcttt tagcaactta tataataacg aaaatggtat taaagtgcag
      301 ttttacgaac ctgttaaaag tattgcccct gggcaaagtg cagtttttta tgaaggagat
      361 gatgtaattg gcggaggaat tatacaaagc aatattttta acttttaact ataccacact
      421 aaattaagtt agtgtatctt gttttgtaaa ttcatatttt atatatcttt ccacccaact
      481 tagtttatga aattgtttgt aagatatagt gttttaattt ttagtttatt ttttgtagcc
      541 tgtagctcca aagaaacaat aaacctgcca acagttgctg aattgaaccc cttacaaaat
      601 ggcaaagttt ttatttacag attagattca acagtaataa ctaatttcag taaatcattg
      661 gccattaaaa gttatcatgc aaaagatagt attgccgata actttgttga tgtaagtgga
      721 aaacaatctt ttaaaattta tagatatatt accgatacat tacaaactaa cccttggatc
      781 tataacgcta ccttttttgt aagtttcaat aaaaacaaca ttgaatttac cgataataat
      841 aatttaagaa ccatcaccat agcatctcct attaaagaag gcacacaatg gtatggctct
      901 aaatacataa atgctccaaa tacttctgcc gattattatt ttttaaaaga ttggtttttt
      961 gaaattaaaa atgccgattt gccattcact gtaaaaaaag gaaccattaa caatacctat
     1021 accattttac aagcagatga agcagaccct ggcgggccat taaacccttc tttacctcgt
     1081 tcttacaggg cttactctgt tgaagtatat gctaaaggca taggcttaat ttataaagaa
     1141 tttttacact gggagtggca aaatcctaat aacgatggag cttataccaa caactcctat
     1201 ggcataaagc ttaatttaat agattacaaa taatatcttc tcagtaacta ttcctacatg
     1261 aaaaaaataa ttattgctgc ttttttttta ataaattatg tatctgcaga agcacaattt
     1321 agtcgttaca ctatacaatt aaaaaataaa ttattcaatt cttacagtac caataatccc
     1381 tctgcttttt tatctcaaaa agcaatagat agaagagtaa aatataatat tgcaattgat
     1441 agcaccgatt tacctataac gcaaaaatat attgatagta taaaacttgc aggcaatgta
     1501 accatattaa ataaaagtaa atggttaaac caaattacta ttttaacaac cgatgcctct
     1561 gccatcaata aaataaccaa tttttatttt gtagagagtg tcaataacat tgcagcaaaa
     1621 cctttaaaag aaaacaaaaa agatacttta caaaaaaata aaaaaataca gttcaaaaat
     1681 aatattacca tttcctacac accttattca aaagaacaag cattaaacaa ttattataac
     1741 tacggactta ctttaaacga aattaaactc aacaatggcc agtttttaca taatataggt
     1801 atgagagggc aaaatatgtt aattggtgtt ttagatgcag gcttttacaa atacaattcg
     1861 ttacctgcaa tggatagtat gaacttaaat aatagagtaa ttgaaacatg ggattttgta
     1921 aataacgaga caagtgtagt agaagacaat tcacatggta tgagttgctt aagcactatt
     1981 gttggtaata tacctaataa ttttataggc aattcgccag aggcaagtgt acttttatat
     2041 agatctgaag aaggtgtaac agaatatcct attgaagagt ttaattgggt ttgtgcatta
     2101 gaaagagcag atagtgcagg agcattggtt attactacat cgttaggtta taccactttt
     2161 gataatgcaa gttttgacca tacttatagt aacatgaatg gtaaaacaac aacagctgct
     2221 aaaggtggaa cttttgcaca tagaaaagga atgctattat tcactgctat gggaaatgag
     2281 ggtacaaaca gttggaaata tttaagcaca cctgcagatt tagatagtac aatttcggtt
     2341 ggtgcagtta acacatctgg caatattgcc aacttttcta gttatggtcc aactagcgat
     2401 ggaagagtaa aacctgaagc ttgtacagtt ggttggaatg cttatgttca aaatccaaac
     2461 aatacaatag ggcaaggtaa tggtacatcg tatgcttgcc caaaaatggc aggcttaggc
     2521 acttgtttgt ggcaagcttt tcctgaatta aataactatg caattagaaa cgcaattata
     2581 aaaagtggaa gcatttttaa taaccctgat gatagaaaag gttatggcat accagatatg
     2641 aaaaaagcat ttgcaattct attacctcag ttttcaaaaa ttaccaatta tagtaataac
     2701 gattgtgcaa ttaatataaa ttggcaaagt aaagatgtag gcagtatgca atatattgtg
     2761 gaaagaagat taaccaacga aagtaattat acagttttaa aaaatataaa tgggtccgaa
     2821 gatattttag caatacacca atacgagttt aaagatacag ttaaaactac tttgccctcc
     2881 acagttgcag tatatagaat taaacaagta atagacacta caactgctgg ttatacaact
     2941 gtaaatatag attcagttag ttttaattta aaccaatgta ttgccagtac agagataatt
     3001 tctttattac caaatcctgc acaaaacaat tgtaccatta aaattgaaac cccttacaac
     3061 atccccaatt taattattag aatcttagat ataaaaggaa acattgtaag ttcacaaaaa
     3121 tttaataaac caattggtgt aagcttattt aaactaccta tctacttttt ggcaaaagga
     3181 atatatatag ttggtgtata taataatgat gaattgatag agaacaaaaa attaattaaa
     3241 caataaaaat gtgtaacttt agctgatatt tattctttaa taactaaact attcctctat
     3301 gaatttatta actaccgagt ggaaagcagg taaagaaata ggtattttaa ttttacgttt
     3361 ggctgcagga tattttattt tatatggcca tgggtttaat aaattagaag taatttttac
     3421 cggtcaggaa ataaaatttt tagaccctat tggtttgggt acaaacctct ctttttattt
     3481 agttgcattt gccgaaggaa tttgtgcttt actaataata ctaggacttt ataccagatt
     3541 ggcagctttg gttttagcct ttaattttat tgttatttta attgtacatt cagcagataa
     3601 ttttatagat atagagcata tttatctcta tttattttcg tatctaactt tgatatttac
     3661 tggctctgga aagcttggtt tggataatta tttattcaat agaaaataat ttagcaccga
     3721 gtataagcat gtactaattt gtatattata catttttaaa caatgctaca aacattgtat
     3781 cagcattatt attataacca actatgtttt gcatttgtac taattgtaat gctgtattag
     3841 caagtaaaaa ttttactata tcctcatttt ctgttgtaaa aacagaacaa gtaatgtata
     3901 aaaaaaagcc attcttgttt aaatgttttg cagcttgcaa tgttattttt tgttgcaata
     3961 gggtgtaatt atttaatttt tcattggtaa aaaattgaat ttgttctggc gttctgcccc
     4021 atgttccact gccactacaa ggtacatcac atactataaa ttggtattta ttaaacttaa
     4081 tatcttgctg ctccttgctt aaatctgtta caaaagattg ataattgttt attccagctt
     4141 ctttaaatct tttagcaaga ttaattaata tactttctct tatatcactt acagttaatt
     4201 taatatttgg aaaagtatct aataataaaa tagattttcc accactagca gcacaacaat
     4261 cccacactaa taaaggttta tgggtagaat aattatttac atttaaagct gataatacaa
     4321 tttcatagta ttgtgcaatt tgttgagagg agtaatcttg tattacaaca tctttattta
     4381 cttgtaatat agcatctacc tttgttgtat ttggcagagc aatacaatta ttgtttaata
     4441 atttatatgc aattgctgct gcttgtagct tttgtaaaac tgtttgtaaa tagtttggcc
     4501 ttactctaag aaatagttga ggttgattaa aaaaagataa aacaaactcc tctttgcaaa
     4561 ttccttcact taataaatcg gtaaaaacaa aaatggaatg gattgaaaaa ttgggataat
     4621 tcttttgtac aaaatctatt ctttgtgcta aggaagaatg ccaatttgca agccaattgt
     4681 tattatataa aatgccatat tcattaattt tattatgaca taaatacaag gcaattttaa
     4741 ttttttcttc aaaggttact tcgtttaagc ttttacctaa cctaaaatag caataacata
     4801 aatgagtaat tgcttttcta tctttactac catgcttttt attttgtgca aaataatttt
     4861 ttaaataatg aacaagagga atgttcccat aataattggt gattaatgat acagccgtat
     4921 ttaggtatga ttggaaacgc atataacgat ttaagtttga aaatgtacaa gtatataacc
     4981 acaaactaaa aactacaaac caccaaccaa ttcaggtgtt cttatttctt caattttacc
     5041 tgctttcatt acctttatcc tgtcacttat atagcgtact acagagtgtg actagtccta
     5101 aaaaaacgat acagggttat tgattacaaa aatagatttt taaacaggag aaagcaattg
     5161 ctttcacctg ttttttgttt tataggtttt catttttctt tctaattgaa agtgcatttg
     5221 attaggagta agcatagcat tggataaatg aggtcttacc gagttgtaaa tttgaataga
     5281 ttcactgaca actttcttta tcaatggtaa atctaaatga agcttatcaa tcataaattc
     5341 ttgctttaaa atatcattta ctcgctctgc tacggcattt tcatatgggt ctgagttttt
     5401 ggtcatactg catttatttt tttgcttttc caacacatac tgatatttat cagaacaata
     5461 ctgaatacct ctgtcagaat gatgtatcaa ttctttttgt ttgtaaattc tgttttagtg
     5521 tgccattttc aaggctctta aactgctttg agttcttaaa gaatcggaca catcataacc
     5581 cactattttc ttggaataag catcggttat caaacttaaa taacacggtt tttttctttt
     5641 gccgatgtag gtaatatcgg atacccatac ttgttcctat tggctaatct ccatctcctt
     5701 aataaaattt gggtgttttc taaaacggtg atacgacatc gtggttttgt gatatgtttt
     5761 ttaggcatta tcaataagtg attagctcgc agaatatcga agaacttatc tctacctact
     5821 ttcattgctt gtaattcttt tgataataga aaatacagct ttattgtacc aagtctgggc
     5881 aataacattc gttctcgatg taccaactgt actgcttgtt ctactacgct ttgcttaagc
     5941 atttttcgtc tgacacccct ataataaact tgtctgtcta tcccgtacaa cctacaacta
     6001 aaacttatgc ttttctctgt tttggctgtg tattcttgga ttgttcaggt gcagagtttt
     6061 ttctgatatc aatgttatat tccttttctg caatatctat cgtcatttaa aaaatgattg
     6121 ccttttggtc ggcaatatgg ctctgatgct ccaataaaga tttttgtttt tctaacaact
     6181 ttactttggc ttccaactcc attatgcgtt gttcaggcgt ttttgacata attgatggtt
     6241 tttggttctc ccagtcaaag ttaccatatt ttctgagcca tagcataact ttagaactgg
     6301 attgaatgcc atacttgcgt tgagcggcaa gggctgagtt gtcctgtttc tacttcttta
     6361 acaacttgca gttttaaagt tagcgtataa tctttctggg tacgctttac ataatttgtg
     6421 tttccttcca taacgattct tttttctgta tcactatttc aggacgggac aatgtaaaaa
     6481 aaggaaagcc ctgaaaaaaa cttttagggc ttttttttat gggagtacga ttttttcacc
     6541 atattatttt tgacggcaaa ataaaaattt taagcctgcc tgataattaa caaaagttcc
     6601 ttttccatac atattggctg tttgtacagg gtctgtattt atttgatagt ttatatcgta
     6661 aatggcatta ttggtaaagc cctgataata attaaaggca gcagaaaatt ttaatttttt
     6721 tgcaaatctg tattcaatgc caattcctcc ttgcaaaagc ataaatatat tcttatctat
     6781 ttctattcgg gtataatgat atagagaatt aaaactgcta ttgccttctt cataatagcc
     6841 ctcacctcca tcttgattta gtgttttaat tacaggtgct aatcctatta aaggaataat
     6901 tgaaaagttt ttatcaagtt taaaattata gcctgccctt agtgagaaca ttattacggc
     6961 atctgaatta gaagtaaacc aagaattttg ctgttttaat ttcaatccat ctgtgtattc
     7021 catagctaaa aaagctattt cataaaacca tttctgtttt tgggtgttaa ttctaaaatt
     7081 tattcctcct ataccacttt ggcatggaac attttgtagg tttttttgag ggtctttgat
     7141 tttatacttt gtataaacaa gtccaccttc tgtaccaatt tcaaatttgt atttctgtgc
     7201 agttgctgtt gttataaaag cagaacacaa caaatacaat aaggctattt gttttatatt
     7261 catatttgta attcaattaa gataaaagag gctttgcaat attacattag ggttgccgta
     7321 aataagaact ttctttgtcg tacatttgaa taaaacaggc tgtatcaaaa aaatcagaca
     7381 cagactgttt tatgaatatt tactttacca atctgtatta gaagaatttc tacagcccgt
     7441 tgtacccaat aaatttaatg atgtagcata aatgccattt ctgccaaagc caaactgcaa
     7501 gtaatgagta gggtttccaa ctactaatgc ataaggttga taaaatttaa tttttacttt
     7561 ttttctttta gaagaaacag gactcttata cacccaatca cctccgtcac agttattcca
     7621 ttcctttaca caaaaatcaa tacttgcctt atttttgcgg tctttttttc gtttcccttt
     7681 gctgtttaat ttccaaatct tagctttacc tttaattttt gtttgtgttt gattaaagtc
     7741 aaataaaacc cctttttgtt tcattacata accaatccat ttgtctgttt cgttaggtat
     7801 ggtaattccg tctcttacgt ctctttctgt tgtagttgta gaacctgcac aatcttccaa
     7861 taatgacgat atttctctta caatattaac cacccctccc aatacaccaa caatatcttg
     7921 gattactcca agtagctctt ttattttaga gtcttctcta ccttccatca tatcctcatc
     7981 aacggtagta ttgctaatga catacctcct atcacaagta atcaatgtcc aatattcttc
     8041 gtcttctatt ggttcttgaa tggtaggtgc atttgcagga ttaaactcta tggaattttc
     8101 aagccaatcg tcctcacttg tttctaaaca cattcttagt gattcaaaac taaacatgtt
     8161 ttcaaaacat tcacttgcca tatcagcagg aaaaataaga taatcctctt caagcatatc
     8221 tgaaaatgct tcttgaccgg cttcatcatt tgggtcaatg ttagtcgtat ttataatatt
     8281 atctagtgct tgcccgtatg cttcattcca attatcattt aacgaatcta ttgcttcggc
     8341 aatttcaaca aatcgtaccc aattaggata agttaatgag ccatcacccg ttggagtaac
     8401 aacttgagca atattagcta cctgcaaaca aatttcgtct tttaagattt ctaagtgaga
     8461 cttttcttcc tctattttag caacaacttc atctgttaat agaggtatat ctgaagcatt
     8521 aatagtttgc ccgctaattt cgtgtgaaac attgttctct aatcttttgt tacatgaact
     8581 tactgaaagt gtaattaaaa tagcataaat gagtgccaat gaaatgtatt ttttcacttt
     8641 tctgattttt ggtttaggtc aaataggaaa atccttaaca tagtttgact tgctacaacc
     8701 tgcatacttt ttaggtaagt tgtcggtgtg tacaaccttt ggcattaaaa taatttcgcc
     8761 tttcttattc ttcgtagtca tttggtaaag ctctttttcg tcctgtattg accgttgaag
     8821 ttagcaggat gtcaaaatta tattgctgcc aacataaatt gttaatcaaa acaataatta
     8881 ccctttcata cacccaattt tttacacaga tattctattc ccctataaga tattttatag
     8941 aaccccatta acttattccg atattttata tttttacagc atggaaacaa taggacaacg
     9001 catagcacaa gaacgttttg ccaaacgttg gacacaagag aaacttgcct ctgaattagg
     9061 cattacacaa agtcttttat ctgatattga aaatgataaa gtttcgccca aatggcaaac
     9121 aatactatct actgccgaaa aactagaagt ccctttttcc aaattattgc ctaatgacag
     9181 taatatttta tatcaaccaa gtatgaatga caattccata ggttttgtac ataattataa
     9241 ctcctttgca gatgaacgta aactatggga agccttactc caagccaaag atgaaataat
     9301 acaaagccaa aaattggtca tagctacttt gcaggagcaa ggtaaaaagg gataatctcg
     9361 tttgttctat tagctttagc tcacagacaa cagaggaaaa atgtaaaata taaacatagt
     9421 ttatagctaa aagctaacag tttttaccaa aaaacagcgt taatcctaac aatcataatc
     9481 atctgtgttc tacaaatacc caatagccgc aaaaaataaa taaaatgtaa aaactaactt
     9541 gcagcatgga atttgcagtg gtgaattttt acaaaaactg catctgttaa tcaataaaaa
     9601 caaattgtat gaaaagtata tggatttacc tatttgttgc attaggtttt atagcagccg
     9661 tttttcctcc tgagcaaaag aaagttgctt taattattgc cattggtgat tatagcgaag
     9721 cacctggatg gagaaattta agcagcatga acgatgttcg ttatgttaaa caagcattat
     9781 taaaagtggg ttttgcaaca aataatatag atactttaat taataaagat gctactaaac
     9841 aaggcatgat taaagcttta gacgatttgt ataataaagt tggtgaaggc gatatagttt
     9901 attttcaatt tagtgggcat ggacaacaaa ttcaagatga taatggcgat gaattagatg
     9961 gatatgatga agctttaata ccatacgatg cttctgcaat gtacgaccca gtaacgtata
    10021 gtggagataa acactttaga gatgatttat tgggtgaaaa attaaataaa ataagaaaaa
    10081 aaataggtcc taaaggtagt ttagttgtac tagtggatgc ctgccatagt ggaacagcaa
    10141 caagagatgc aggtataaaa cgtggtatgg aaaaaccatt tttaaccgac cccaaatatg
    10201 taccaaacat taaaattaat ttaaacaaag aaggtgagca tggattatta gaaggcttta
    10261 caaatggcat gggaaatatg attgtattta gtgcaagtag cccaaaccaa cctaattatg
    10321 aaatgaaaga taataataac aatggcgtag gctctcttag ttatgctttt gcaaaatcta
    10381 ttgcagaatt acctgcagaa agcagttacc gagttttatt tcataaaatg aaagcaatta
    10441 tacaaggaga tattcctaac caaataccaa tggttgaagg cgatttagac ttagaagttt
    10501 ttggaggtaa atacatacca aaacctacta taattgctgt tgatcaaaaa tttaataatg
    10561 catctaataa gtttaatgat accacttttg ttattagtat tggacaatta aacagtattt
    10621 ataaaggcac aacattaaaa atttatccag ttggcagtgc agaagcttat tgcgatgcag
    10681 tggtaacctc tgtaagctct tttcaaagta tatgcaaaag caacaagcct ttgaaaaaaa
    10741 gtgttgccta tgaagccaaa gtagaagctg caagtgcagg agactttgca gcagaattga
    10801 tgattcaaaa caatgaaacg aatagtaaaa tcgctaatac cgttgcatct agttttagca
    10861 attttgtaaa gccttatcaa tatttatctt ttagtaataa ccccgatttt actttagata
    10921 tttccaaaaa caacaatggt ggctacacag taagcttgtt aggaaaaggt gatgatgtac
    10981 gatatacaaa aactattaca gacagtttat ctatagaaga ttgtaaatat tttttagaag
    11041 gtataaaaag aaatatgagg gttaaatatt taagaaattt accagatggt ggaacccttg
    11101 ctaaagatat taaagtagaa attgttccta aaaaagcaac caataatcaa aacgaaattg
    11161 taatgagtgg taaagattta tttgaaatta gaattaccaa tacaggtaaa tcagattact
    11221 actatactat cattgatatt atgccagaaa atgaaatgaa agttttatta ccagatgaaa
    11281 ctagtgagcc acaagatttt gtaatacaac caggacaaac cattccaata ggcgacattg
    11341 aagttgatga aactacacct aatggaaaag aaatttttaa aattattgta agcaaaatac
    11401 caatggattt aagaaatgtt gtaaatagaa ctcgtacacg tagtgccaac gcagcaatga
    11461 aatctgttga aagtgtaata gacgatttat ttaaagacag caatgatcaa agagctactc
    11521 gttcatctat cagtaatgta aaagttgatg aagtaggaat tttaagctgt ggatttacag
    11581 taaaaaataa atagtaattt ataaaccaaa gcagtattat ttggtttaat actgctttat
    11641 ttttttgaat tgactttaaa acaacatttc ccttctacca atattccacc ttatacctgc
    11701 cgataaaata tttgtattgg ttatgccagc agttttaagt ttaaaatttc tggttaaata
    11761 agcaatatct ataaataagt tttcttttat ttcgtaactg gcaactaaag aaattaaagt
    11821 acagttagcc aaatttcctg taccaatttt ccagccattt tcaaatggcc ttgtattcat
    11881 aaaattaaaa atattgctgc cgtaattttg attaatattc aatatactac tatctaaacc
    11941 ttgtttgtaa taaatagctt ttgcttctac atacaatttt ttaataggct gataacgtat
    12001 aattccgata tactctttaa aattggcacc taatggatgt gccaatggtt gattataatg
    12061 attgtaactt gtaacagaat cgttagctgc ataagtaaac ggacgagcaa cattagtttc
    12121 tacttgtaca tctaaatttt ttattccaaa agcatctgta tattttaaac ctgcctgata
    12181 ggcaaatcta tttccccacc agtcattttt taactcatct gtttttaatt tatctatcat
    12241 taattggcca tataactgaa accttttggc aatatttgct tttacatcaa agccaatata
    12301 cgatttatca tttttgtttc cagatacatt ggtaaacata attggattaa ataatggcaa
    12361 gctaactgca ctattatctg ccaacattaa tccttcaaac aaacctaagt ttaaccactt
    12421 tgtagcttta aaatttaaat aacttgctct aaagtattta cgaggataaa ttctgtcgct
    12481 accagatttt ttataagaaa ccaattcggc aaaaatattt tggaattgaa attttccgaa
    12541 atgagtatat actttaaagt acatatagtt ggtactaaaa tcacttaaaa acaaactcct
    12601 gtaaccatta cctataaagt ttctatcata agccaattgc atatccataa acttggcaac
    12661 tttccagctt aacccacctc taatatcaaa gtaatcgtac ccaccggttt taaaattttt
    12721 tatataacct gctccaggat aggcaataaa tttatttttc caggctgcaa cataatctcc
    12781 taatctctcc tgattttcgg taaaataaaa gtgaaaccca attttagtac caattaatcc
    12841 acgaacatta atacctcttt tattaataaa ggtgctttta gaatttcctc ccttacttcc
    12901 ttgttcatat tgcaatattg gattagcaat taaaacaaaa tcgctgtttt gcaattcaat
    12961 catattagaa cgattatgga aaaagtattt ccaaattggc ttttcgcttt taaaaatttc
    13021 tttgggttta ctccattcac tattagccat taaaaagcgt tgcaaattat atttatctgt
    13081 tgaagttaaa acagtggcat ttttatttcc tgctgtgtat aaactatccc aataatcagc
    13141 agtgttagta atatattttc tgttaaatgg ttttaatgat gaaaatgata aaccattggc
    13201 attcgttttt atttccattc tattcaataa ccattcttct tttccgccta atgttaagta
    13261 actgctttgg gccaaaagca aagcaggcat gataaaagac aaggtaaaaa ataatttttt
    13321 cactactttc attattaaat tatttgtaaa tatttttatt aaaattcaaa ttgctgttta
    13381 gtaatattcc acctaaaccc tacattaaat attactgtat tattagttgt gccagaggca
    13441 agtttataat ttctaaaaac aataccagca tcaaaaaata aattttcttt tacctcataa
    13501 gctatgttgg tattaaaata aatgcaagtt gctttattac cagacccaat cttccagcca
    13561 taattggttg gcctgtcttt gtaatctctt aaaatattat taccaaaatt tataccagcc
    13621 gtatcttgcc cttgatagta ataaataaac ttagcgtcta aaaaaagttt ttttaaaggt
    13681 tggtatttgg ttgctacaat atactcttga aagcctgcac ctaatggatg tgctaaaggc
    13741 atattattgt tgctatagtt agaaactgaa tcgaagtgtg tataagtaaa tggtcttgct
    13801 ctatttattt ccacttgcat gtctaagttt ttaatgttaa gtacatctaa atattttaca
    13861 cccaattgat acccaaattt atttgcccac caacctctgt tggctttaat ttctcctaat
    13921 aaaaactcat ctagcagcac ctgcccataa agctgaattt tattttttaa attggctttt
    13981 acatttaagc ccattaaagc attatctggg ctaccacttt gttgttccat tgctcttaaa
    14041 aaagtaaacg gaagcaaata atttaattca aactgatttt ttctaccaaa aactactgca
    14101 tcaaaaatgc caatatttaa ccaattggtt gcatctatgc ttaaagtgtt aatacgtaaa
    14161 tattttttaa aattagcttt ggttgtaata ccaaactgag gggtgagttg catatacaaa
    14221 ctttcaaaat tcaacttcca taacctaatg tttgatttaa agaataaaga gttaccacta
    14281 aaatcactta aaaataaact tctaattcca ttgcctataa aatttctatc gtacccaact
    14341 tgcatatcca taaacttagc taatgtccat tgtgccgatg ctcttacatc aaaatattgc
    14401 actgcattac tatcggtaaa attaaaatta ctaaagcctg gtacagcttt aaacttgctg
    14461 gtataggctc ttacatataa aggggttctc tctctattgc ctgttgcata aatattaaaa
    14521 gctatttttt tagcaaccaa tccagttgca ttcaatccaa cccgcatact atataaactt
    14581 tgtttatctt ttgtttcggc accatagctt acttgtaaag caggattaat agtcataaaa
    14641 aacttgggct tattaatttc tattaaattg aaagcagatt ttttattatt gaaggaagag
    14701 gttaaagaag tgtattcctt atgctgcatt aaaaaacgag taatattgta tttatcaaca
    14761 gtagataaac tggccacaat tctgttattg ttcctttgta aactatctat tgtttctact
    14821 tcttttacca ataaccttat attatatggc ttaataaagg aagctccaaa agcaggcaat
    14881 ttagacttta tctctaacct atctaatata ttatattctt tatgccccaa tagagaatag
    14941 cttgtttgtg ccgaaacact ataacaaaat aaaacaaaca gaattataac aatagttttt
    15001 atactttgca tcataaatat tgaaaaattg ttgtcaaaaa taaatgctat gaatcattat
    15061 ttaattaaag atacacatat agtaaacgaa ggcaagattg ttagtggtga tgtgttgata
    15121 aaaaatggaa ttatagaaaa agttggagga gttattaaca ccaaaaataa ccccattgaa
    15181 gtggatggaa gtaaccaaca tttattacct ggtattattg atgatcaggt acattttaga
    15241 gagccaggtc ttacacataa agcaactatt tattccgaat ctaaagcagc agttgcaggt
    15301 ggcgtaacca gttttatgga aatgcctaac acctcgcctc ctacttttac acaagctttg
    15361 ctagaagaaa aatatgcaat tgcaaaacaa acatctttag ctaattattc attttttatg
    15421 ggtacaggaa atgataatta tgaagaagta atgaaaacaa atgagaaaaa gaatgaggta
    15481 tgtggtataa aaatttttat gggtagtagt actggaaatt tattagtaga taacccaatt
    15541 ttattagata aaatttttgc aaatgcagag ctgcttattg ctacacattg cgaagaagaa
    15601 tcaattatta aaaacaatct ggaaaaatta atagcaacaa aaaccaactt agaagcagca
    15661 gaccatgcag ttattagaaa tgaagaagct tgttttgaat gttcatttaa agcaatacaa
    15721 cttgcacaca aacacaatac caggttacat attttacaca tcagtaccca aaaagaactt
    15781 cagctttttg gaaatattgt accattaaaa gaaaaaagaa ttactgccga agtatgtgta
    15841 catcatttac actttactgc taatgattat gcaattttag gtaataaaat aaaatgcaac
    15901 ccagccatta aagctgcata caacaaagaa gcactatggc aagccttatt aaatgataaa
    15961 ctagatatta ttgctaccga ccatgcccca catactattg aagaaaaaaa tggaacatac
    16021 ttaaaagccc atgctggctt acctttagta caacattctt taatgttgat gttacattat
    16081 gtacaacaag gtaaaattac tttagaaaaa gtagttgaaa aaatgtgcca ctcagtagca
    16141 gattgtttta ctataaaaaa cagaggctat attaaagaag gttattttgc cgatttggtt
    16201 ttagtaaatt gctaccaacc ttataccata aacaaagaaa atattttata caaatgtaca
    16261 tggagcccat tagaaggttt tacttttcct gcaacaatta cacatacttt tgttaatggt
    16321 aatgtagtgt atagtaatgg tgtttttaag gaagagttta aaggaagtag aatattgttt
    16381 gatagaaaat aattagctca tattttatac attgaaaatg caaacagata aagtaaccct
    16441 gcaagattta tctattttta atgccgatga aagtttatct gttttgcatt acttaaactt
    16501 tactaccact aacagaggaa aaccttttct atacaatatt ttaagccatc ccttatctac
    16561 cattgaagaa attgaagata cgcaaacaac cattcaatat ctaaatacat taataccaaa
    16621 atggaacatt aaaataacca atggcacttt aatggttgtg gaaaaatttt atgaaactgc
    16681 atttaaccat tacccaacaa accctagtac aataaatagt tttttttata aactaaccaa
    16741 taatgcagac ttctctttaa ccaaatattc cgtaacacat tgtatttatt ttttaaaagg
    16801 aataaatcaa gttttattgt ttctatcaga tgcaaaaagc aaaaagctac ttttttacaa
    16861 ggaaaaacta agcttaattt taaacaaaac tattgtacaa gaaattattt tgatagatga
    16921 tgtaaagaag attaaaaata ctagcatatt aaaatatgca tactttttaa aacatgaatt
    16981 caaacataat atgcagcaac tcattgttgt gtttgaacaa ttagatgcct atttaagttt
    17041 agcaaaagcc tgcgaaaaat ttcaattaac ttttccaaaa tttattgaat ctgatcaacc
    17101 atttattgaa gctgaaggtt tgtttcatat tttattacca aagccagtaa gctatactat
    17161 ttgtttaaac aattcagaaa attttttatt ccttacaggt gcaaacatgg caggtaaaag
    17221 cacttttata aaatcggttg gcattgctgt ttatttggca caacttgggt taggagtgcc
    17281 tgccaaaaaa atgaccatta gtttgttgga tggtatgcta agcaatattc aaataagcga
    17341 taatattatt aatggcgaaa gttattttta taatgaagta caaagaatta aacaaacagt
    17401 ggagaaaatt tctggcaaca aaaaatggct agttttaatt gatgaacttt ttaaaggtac
    17461 taacgtacaa gatgcaatga aatgtagtag tattgttgta gaaggtttat taaaatctag
    17521 caaaacatta tttatcctat ctacacattt gtacgaaatt gccgaagatt taaagaagca
    17581 ttcaaacatt cagtttaaat ttttagaaac aacccttgaa aaggaacaat tagtatttaa
    17641 ttatcaatta agaaatggaa ttagcgaaga tagaattggc tatgtaattt taaaaaaaga
    17701 aggtgtaatt gaaatgctaa ataaactaaa ctaattttta ttctttttaa aacttagctt
    17761 aaaatatagc aatactaaag acaaggctaa tactataaaa tttgcaacaa taaccggacc
    17821 tccaccttta attccaaatc cataaacaag ccacactata gtactgatga ttacaataga
    17881 aatcatccaa atacttaaat ctcctacgct tttggttttc catgcttgaa aaacctgagg
    17941 aagaaaagtt atagatgtta ggaatgaacc aatataacca attaactcaa tttgagagga
    18001 taaaaaagta aaaagtaaat tcataggttt ataaaacagg ctgtttcaaa aaaaatattt
    18061 tttttgaaac agcctactaa tttattgttt tactaatttt tctgtgcttc tattgccttt
    18121 gctgtctgtt ac
//
LOCUS       DAOQHP010000005        17805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1466_length_17805_cov_3.720000, whole genome shotgun sequence.
ACCESSION   DAOQHP010000005 DAOQHP010000000
VERSION     DAOQHP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17805
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_17805_cov_3.720000"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLY81_00410"
     CDS             <1..567
                     /locus_tag="PLY81_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNF28720.1"
                     /translation="DTSAYKITFTGTSVTFLEGSSFIISGLKESILYAFLLIAFCMLY
                     LFKSVRILFCSLIPNIVPLLLTAGLMGWAGIRLTPATVLIFSVALGIVIDVTIRFLVN
                     YKQELPHYNNQTIPTLIQTIKHTGISIIYTSLVLIAGFIIFSFSGFNSTQSLGWLTSF
                     TLVTGTITNLLLLPVLLISLQKKSKEKS"
     gene            579..1700
                     /gene="hemW"
                     /locus_tag="PLY81_00415"
     CDS             579..1700
                     /gene="hemW"
                     /locus_tag="PLY81_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNF28721.1"
                     /translation="MAGIYIHIPFCKQACHYCNFHFSTLHNNMPEMLDAIIKEASLQK
                     NYLQEAIETIYLGGGTPSIYKPSDIEKIITHLHQTFTVIPNVEITLEANPDDITEEKL
                     QQWKNIGINRLSIGIQSFVEEDLKWMNRAHNSFQALHCLQIATKYFNNITVDLIYGTP
                     YLTNEQWKKNIDTVVQFNIPHISCYALTVEPKTVLHTLIQKNKLPNIDIEKQAQHFEI
                     LTNTLAKAHYEHYEISNFATSGFRSKHNSSYWQGKHYLGFGPSAHSFNGISRQWNISN
                     NALYFKSIAQNKVLYEIEILTTKQQLNEYIMTALRTIEGISLQHVKENFSEEEKEKLL
                     SRSKKLITQQLLSIENNFLKLTPQAKFLADGIAADLFVD"
     gene            complement(1714..2349)
                     /gene="pnuC"
                     /locus_tag="PLY81_00420"
     CDS             complement(1714..2349)
                     /gene="pnuC"
                     /locus_tag="PLY81_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNF28722.1"
                     /translation="MTFEIFTQQLVSGVQQTQWYELVAVFAGIASVWFSRKENILVYP
                     VGLINTIMYVYISFKHHLLGEATVNFYYTIMSIYGWWLWTRKDKQSHEIVLHISYSTK
                     KEWIEQLCFFAVVFAICFTALHLAKQYFFEGVIPAADAFATATAFTGMWLMAKKKVES
                     WWWWIATNITSIPLYFVKSLVFTSVQYIVLLVLAIAGLITWMKKAKAENKY"
     gene            complement(2339..2956)
                     /locus_tag="PLY81_00425"
     CDS             complement(2339..2956)
                     /locus_tag="PLY81_00425"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNF28723.1"
                     /translation="MLQPYYQENIIEAGCDEAGRGCYAGPVFAAAVILPNHFFHPLLN
                     DSKQVKEKDRNELRWYIEQNALAYAVSKIDNNEIDKINILKASFKAMHLALDNLKLKP
                     DLLLIDGNRFLQYKKIKHHCIVKGDGKFASIAAASILAKTYRDEFMQQLHLEFPVYGW
                     NKNKGYGTLQHRKAIEQFGLSKYHRKSFNIVPKQIELFKKVSNDF"
     gene            3146..5383
                     /locus_tag="PLY81_00430"
     CDS             3146..5383
                     /locus_tag="PLY81_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28724.1"
                     /translation="MKKYFVGFWYSMPVQLLFLHFRKYQIFLVLWYVLFATVGGIFLN
                     NFGASSLFLAPEYFNEVSFSSTAIVGFSIGVFIMSWNITTFILHGKHIQFLASTAQPF
                     LKYCINNAVLPILFLIFYFFSALQYNNQKELKPIVEIALLSSGFLIGFFLSITISFMY
                     FFSADKRIYKNMATVIHEANKNYAIAFTENPLPKEKSQIRIDWFFSARMHLRKPRDIR
                     HYSTDFLDRIFKQHHLAAILAFFLAFIALIALGFSSDSRVFQIPAAASVTIFFALIIA
                     FGGAFTLFLKSWSIPIIILLYVLLNSLYKYNILDLRNKAYGLNYENKNNRPEYSKESI
                     YALASKENIENDKLTFLKRLLHWKANQTEDLPILYIINTSGGGVRSSTFTFSVLQMLD
                     SVVFNGNLMKKTFFINGASGGMLGAAYYRELYFRKQQGFNINLQNKQYLDDISKDLLN
                     PIFSSFVSRDIIGPVQKFKKFGYEYVKDRGYAFERSLNVNTRGYLNKRLIDYAEVENK
                     AIIPTMFFNSTISTDGRKFVMCSQPVRFLMNHVNANDLSSEPAPDIIDFNSFFSKQNS
                     NNIGILSALRMNATFPYVLPNVWLPTNPVIDVMDAGLRDNFGQENTLRFIEVYKNWLK
                     ENTSKVVIIQIRDRSINDWDEVEKNKSLWSAFTKPAFLLQYNWYRLQDYYQHAQLEYM
                     NESYGSNLQQVSFQYVPSNKSKAASLSFHLTTNEKKEILNSLHNKVNVDELTKLKNFL
                     VEKED"
     gene            complement(5442..5837)
                     /gene="mscL"
                     /locus_tag="PLY81_00435"
     CDS             complement(5442..5837)
                     /gene="mscL"
                     /locus_tag="PLY81_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNF28725.1"
                     /translation="MMKEFKEFAMKGSLIDLAIGFVMGAAFTKVTGAFINGMVMPLVG
                     MIQGKDLSDWKFVLKDADKVAGTEEVAIKYGSFISVTIEFIIVAFVMFMVIKAINRMK
                     KKQEEAPAAPAEPSSTDKLLIEIRDALKK"
     gene            6082..8946
                     /locus_tag="PLY81_00440"
     CDS             6082..8946
                     /locus_tag="PLY81_00440"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF28726.1"
                     /translation="MKKILFAFICLMLIQVVHAQNSTSLKGKVTDTLSKQNLKDASIS
                     MLNAKDSTVESFGLAKTDGTFEIKGFAMGSYLLQISFNGYESYFKSVVFNKQNPTLDL
                     GTIILKPQAKELEGVSVVAAPIQVKGDTTEFNAGSFKTKPNATAEDLLKKLPGVQVEK
                     DGSVKAQGQTVKRVLVDGKRFFGDDPTTATRNLPTDVIDKVQVYDAQSDQSSFSGFDD
                     GNRDKTINIITKKDRRKGYFGKLSIGAGNNERYATNANFNRFNGNQQISFIGQGNNIN
                     QQNFSMQDLFGTMNSAGGRGGGGMFGGGMRGGNLGNFLTTNQAGIATTWAAGLNYNDV
                     WSKKTSVSGSYFYNNMKRNNDNESHTETFVTNDSSQFNNNNTFSNYLNQNHRFNFEFD
                     HKFDSVNSLLVRPNFSYQISDNNQQTISTLTKGKLVNLSNVQQVTNSQNTGYNFSTSI
                     LLRHKFKKRGRTISLNLTPGLSNNESDGSNISYNDIYTINGIVKDTINQINSNDRNGR
                     TFSSNLSYTEPITKKSQLELSYNYNYSKNNSNQQTLMYNKQTNSFDIIVPNLTNKFEN
                     TNTSNKFNANYRVQLTKEWSYTLGMGVQFAELTSINNTKATKLSNTFTNYFPSVQLHY
                     SKNRTKNLRLVYRGQTNAPSITQLQDVIDNSNQLNIRNGNAGLEQEFQHNFNLFYTNF
                     DVITFKNFFVSINGSFTQNKIGNSVIQNTGTTPTVVDGIVLIPGAQYTKPINLNGAMN
                     IFGFINYGFPIKKPKSNLNFSTTMSYIKDVNLYNQTKNYTHNYIIGERVNYNMNVKEV
                     FDVNFASSSSYTFARYTLNNEQNGDYFTQTFSIEPTYSSKSGWIIGSDFDLTINRGQS
                     VGYNQTIPLWNASIAKTIFKNKSGEIKFSVFDLLNQNKSINRTVEQNYIQDTRTQVLT
                     RYFMLSFTYNLRKFAGKGQQMPPFMRGMMRNAQPRMMRMH"
     gene            9012..9737
                     /gene="tsaB"
                     /locus_tag="PLY81_00445"
     CDS             9012..9737
                     /gene="tsaB"
                     /locus_tag="PLY81_00445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNF28727.1"
                     /translation="MAYLLYIDSVFDTANIYLANNEQIIFADKSLEQKNHASFIQAAI
                     QKNFQKTQINLQSINGIVVVNGPGSYTGVRVGLATAKGLCYALNIPLVLLNTLDIMAF
                     EMKSNLSKFIPLANSTLPILFCPMIDARRMEVFTALYNHNLNYIIKPCASVLNEFSFE
                     NELKENTIFFSGNGSNKLKEVLKNPNAIFTDLLFQASTLIQLGLNQFNKNNFSDLAYS
                     QPFYTKEFYTTASAISKIKGLDK"
     gene            9802..10131
                     /locus_tag="PLY81_00450"
     CDS             9802..10131
                     /locus_tag="PLY81_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF28728.1"
                     /translation="MNQTNIVLSNGELSLQIDPLRVKKSAMVIRALNHKLRQQMIKLI
                     DEVKTITVTDIYVRLRLEQSVASQHLAVLRKVGIVATKRDGKKIFYSVNYHRLQHILN
                     TSNNLLN"
     gene            complement(10171..10770)
                     /gene="yihA"
                     /locus_tag="PLY81_00455"
     CDS             complement(10171..10770)
                     /gene="yihA"
                     /locus_tag="PLY81_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNF28729.1"
                     /translation="MQIKTAKYLISSPTFDKCPVADKPEFAFIGRSNVGKSSLINMLV
                     NQKNLAKTSGTPGKTQLINHFIVNDSWYLVDLPGYGYAKRSQKDRNRWEQMIEHYMRK
                     RENLINVFVLIDSRHSPQKNDLDFLDKLGKWQIPFVLVFTKADKEKPLVVKRNEQLFL
                     NKMRETWQFLPQSFITSTEKKEGRLQLLQFIDDCVEEQQ"
     gene            10912..11919
                     /gene="gap"
                     /locus_tag="PLY81_00460"
     CDS             10912..11919
                     /gene="gap"
                     /locus_tag="PLY81_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNF28730.1"
                     /translation="MVKVAINGFGRIGRLVFRQIYNMQGIDVVAINDLTSPAVLAHLL
                     KYDSAQGRFNAEVSATEDAIVVNGDTVKIYAQKDPAQIPWGSHGVDVVIESTGFFTDK
                     DKAAAHLTAGAKRVVISAPATGDLKTVVYNVNHSILDGSETIISCASCTTNCLAPMAK
                     ALNDNFGLVAGSMTTIHAYTNDQNTLDAPHPKGDLRRARAAAQNIVPNSTGAAKAIGL
                     VLPELKGKLDGGAQRVPTITGSLTELVSVLNKKVTIEEVNAAMKAASNESFGYTEDEI
                     VSTDIIGINFGSLFDASQTKVITQGDTQIVKTVSWYDNEMSYVSQLVRTVKHFAGLIS
                     K"
     gene            12095..13294
                     /locus_tag="PLY81_00465"
     CDS             12095..13294
                     /locus_tag="PLY81_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNF28731.1"
                     /translation="MSKFSNHQFANQKALIRVDFNVPLDKTTLAITDDTRMKAAIPTI
                     QKILNDGGSVILMSHLGRPKEGPEDKYSLKHLVQHLSNLLGGKTVFFANDCIGEQATL
                     TASMLKAGEVLLLENLRFYKEEEKGDTAFAEKLSKLGDVWVNDAFGTAHRAHASTAII
                     AKFYAKENRFFGLLMEAEVKAAEQVLHKAQKPFTAIIGGAKVSDKILIIENLLEKATD
                     IIIGGGMAYTFMKAKGGSIGGSLCEDDRLQTALEILEKAKQKNVSIHLPEDSIIADKF
                     AADANTQLTNSNAIPTGWMGLDIGTNAATKFASVINSSKTILWNGPMGVFEMEKFQAG
                     TKAIAVAVAESTQNGAFSLVGGGDSVAAVNQFGFNDKVSYVSTGGGAMLEYFEGKILP
                     GIAAVNE"
     gene            13287..14912
                     /gene="nadB"
                     /locus_tag="PLY81_00470"
     CDS             13287..14912
                     /gene="nadB"
                     /locus_tag="PLY81_00470"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNF28732.1"
                     /translation="MNNKTNQMQTDFLVIGSGIAGLTFALKVANHFPDKKITVITKTN
                     SDETNTKYAQGGIAGVIDETNDSFEKHIEDTLIAGDGLCNKEIVEIVVKEGPARIKEI
                     INYGARFDKNEKGDFALGKEGGHSEFRILHHKDVTGKEMERALLQAVTNTKNINIINH
                     CFVVDIITQHHLGYLVTKSTVDITCYGVYVLNLNTNLIEKIESKITLLATGGCGQAYR
                     ATTNPKIATGDGIAMVYRAKGRIENMEFIQFHPTALYEPGISPSFLITEAVRGDGGIL
                     RNKKGEAFMEKYDSRKDLAPRDIVARAIDNEMKFEGTEHVFLDCTHLPKEKFVNHFPN
                     IYQKCLSIGIDVAKSYIPVAPAAHYSCGGVKTNAFAQTSIKNLYACGECASTGLHGAN
                     RLASNSLLEAMVFAHRAFENIVENASEIFERKNIIIIPDWNTKGTIEPREMILITQSF
                     KELQQIMSDYVGIVRTDVRLARAMKRLDLLFEETEQLYRSTKVSPQLCELRNLITTGY
                     LIVKGADFRKESRGLHYNTDHPCKSDIIQNTIL"
     gene            complement(14920..15876)
                     /locus_tag="PLY81_00475"
     CDS             complement(14920..15876)
                     /locus_tag="PLY81_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF28733.1"
                     /translation="MEHIIFYVVILIFILSFFGLFFIVKQQTAQIVERLGKFHSVRNA
                     GFQLKIPFIDTIAGRLSLKIQQLDVIIETKTKDDVFVKLKVSVQYVVIKDKVFEAFYK
                     LDNPMSQITSYVFDVVRAEVPKLRLDDVFEKKDDIAIAVKGELQEAMNTYGYDIIKTL
                     VTDIDPDVEVKQAMNRINAAERDKVSAQYQGDAQRILIVEKAKAEAESKRLQGQGIAD
                     QRREIAKGLEESVIVLNKVGINSQEASALIVVTQHYDTLHSIGSNNHSNLILLPNSPS
                     AAGEMLNNMVASFSAANIIGKELKKMPNTTTTESNELTTNLE"
     gene            16086..17348
                     /locus_tag="PLY81_00480"
     CDS             16086..17348
                     /locus_tag="PLY81_00480"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNF28734.1"
                     /translation="MVDVILGLQWGDEGKGKIVDFFAPHYDVIARFQGGPNAGHTLYV
                     DGKKVVLHQIPSGIFHQNCVNLIANGVVLDPVTLKRECETVASFGIDVKKNLFISEKT
                     NLIIPTHRALDKASELQKGEGKIGSTLKGIGPAYMDKTGRNAIRVGDLLSKNFTTQYI
                     KLRLKHQKLLDNFHFIEDITAWEEEFFEAIDFLKTLNIVNGEYFINNKLNEGKKVLAE
                     GAQGTMLDIDFGTFPYVTSSNTISAGVCTGLGVSPQKINDVIGVTKAYCTRVGSGPFP
                     TELHDSVGDELRKIGNEFGATTGRPRRCGWIDLVALKYTCMLNGVTKVVMTKADVLDT
                     FENLQVCTSYTIEGKTTQEIPYQISKVAIDPTYQTFKGWNCDSTKINSATNLPANMVT
                     YIKFINEFIGAPVKYVSNGPGREQIIVI"
     gene            17497..>17805
                     /locus_tag="PLY81_00485"
     CDS             17497..>17805
                     /locus_tag="PLY81_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28735.1"
                     /translation="MAAKSDKGKKSVKPETKEIKESKKTSSKPKKQLEEEDEEDNFED
                     DLDSPSTSKKSSKKTPSKKSKEEDDDDDDDDDEDKDDWEKVEEEENWDPDFDEFDIPK
                     A"
BASE COUNT         6591 a         2723 c         2723 g         5768 t
ORIGIN      
        1 gatacatctg cttataaaat tacgtttact ggtacaagtg ttacattttt agaaggtagt
       61 agttttataa tttctggttt aaaagaaagt atcctttacg catttttatt aattgcattt
      121 tgtatgctat acctttttaa atctgttaga attttatttt gttctttaat tccaaacatt
      181 gttccattat tattaactgc aggcttaatg ggttgggctg gtataaggtt aacacctgca
      241 actgttttaa tttttagtgt tgctttaggt attgtgatag acgtaaccat tcgtttttta
      301 gttaactaca aacaagaatt accacactat aataaccaaa ccataccaac acttatacaa
      361 actattaaac acacaggcat aagcattatc tatacatcat tggtattaat tgcaggtttt
      421 attattttta gttttagtgg ttttaatagt actcaatctt taggttggct aacttctttt
      481 acattggtta ctggtaccat aactaattta ttattattac ctgtattatt aatttcatta
      541 caaaaaaaat caaaagagaa aagctaattt tttaagctat ggcaggcatt tacatacata
      601 ttcctttttg caaacaagcc tgtcattatt gcaactttca ttttagcaca ttacacaaca
      661 atatgcctga aatgcttgat gctattataa aagaagcatc attacaaaaa aattatcttc
      721 aggaagctat tgaaacaatt tatttaggag gtggcacacc aagtatttat aaaccttctg
      781 atattgaaaa aataattact catttacatc aaacatttac agtaataccc aatgttgaaa
      841 taaccttaga agcaaatcca gatgatatta ctgaagaaaa attacaacaa tggaaaaaca
      901 ttggcataaa cagattgagt ataggtattc aaagttttgt agaagaagat ttaaaatgga
      961 tgaatagagc acacaacagt tttcaagcac tacactgttt acaaatagcc acaaaatatt
     1021 tcaacaatat tactgttgat ttaatttatg gaactccata tttaaccaat gaacaatgga
     1081 aaaaaaacat tgatacagtt gtacaattta atattccaca tatttcctgt tatgcactta
     1141 cagtagagcc taaaacagtt ttacatacat taattcaaaa aaataaatta cccaatattg
     1201 atatagaaaa acaagcacaa cattttgaaa tattaaccaa taccttagct aaagcacatt
     1261 atgagcatta cgaaataagt aattttgcaa caagtggttt tagaagtaag cacaacagca
     1321 gttattggca aggaaaacat tacttaggtt tcggaccatc tgcacattca tttaatggca
     1381 tttcaaggca atggaatata agtaacaatg ctttgtactt taaaagtata gctcaaaata
     1441 aagttcttta tgaaatagaa atattaacaa caaagcaaca gttgaacgaa tatattatga
     1501 ccgctttacg aacaatagaa ggaatatcgt tacaacatgt taaagaaaat tttagcgaag
     1561 aagaaaaaga aaaattatta agcaggagta aaaaattaat tacccaacaa ttattaagta
     1621 tagaaaataa ttttttaaaa cttactccgc aagcaaaatt tttagcagat ggtattgctg
     1681 cagatttgtt tgtagattag aaataacaca tacttagtat ttattttcag cctttgcttt
     1741 cttcatccaa gtaattaagc ctgcaatagc caatacaagc aatactatat actgaacact
     1801 agtaaatact aatgatttta caaaatataa tggtatagat gtaatattgg tagctatcca
     1861 ccaccaccaa ctttctactt tctttttagc catcaaccac atacctgtaa aagctgtagc
     1921 agttgcaaaa gcatctgcag caggaataac accttcaaaa aagtattgtt tagctaaatg
     1981 taatgcagta aaacatattg caaaaacaac agcaaaaaaa caaagttgtt ctatccattc
     2041 ttttttagta ctgtacgaaa tatgcaaaac aatttcatgg ctttgtttat cttttctagt
     2101 ccaaagccac cagccataaa tgctcataat ggtataataa aaattaacag ttgcctcgcc
     2161 taacaaatgg tgcttaaaac taatgtacac atacataata gtgttaatta atccaacagg
     2221 atacactaaa atattttctt ttctgctaaa ccaaacactt gcaatacctg caaaaactgc
     2281 aactaactcg taccattgtg tttgctgtac tcctgaaact aattgttggg taaatatttc
     2341 aaaagtcatt gcttactttt ttaaataatt ctatttgctt gggtacaata ttaaaactct
     2401 ttctgtggta tttacttagt ccaaattgtt caatggcttt tctgtgttgc aaagtgccat
     2461 agcctttatt tttattccag ccataaacag gaaattcaag gtgtagttgt tgcataaact
     2521 catctctgta tgtttttgct aaaatacttg cagcagcaat agaagcaaat ttaccatcgc
     2581 ctttaacaat gcaatgatgt ttaatttttt tatactgcaa aaatctattg ccatcaatca
     2641 gtaacaaatc tggctttaat tttaaattat ctaatgcaag atgcatggct ttaaaacttg
     2701 cttttaaaat attaatctta tctatttcat tattatcaat cttacttaca gcataagcca
     2761 aagcattttg ctcaatatac catcgcaatt catttctatc tttttctttt acttgtttgc
     2821 tatcatttaa tagagggtga aaaaaatggt tgggtaaaat tacagcagct gcaaaaacag
     2881 gacctgcata acatccacga ccagcttcat cgcagccagc ctcaattata ttttcctgat
     2941 aatatggttg cagcatattg caataataca ctttatactc tgttctttca atagtttact
     3001 ttcaacaaaa acctcattag gcaggtataa aataaaatat taacaatagg caattcaaaa
     3061 taaaaattca gtatctttaa aagaaataat tattacaaaa atgagtgagg gttaattaat
     3121 attagtttgg taaatacatt ttagaatgaa aaaatatttt gtaggttttt ggtacagtat
     3181 gcctgtacaa ttactgtttc tgcattttag aaaatatcaa atatttttag ttttatggta
     3241 tgttttattt gcaactgttg gcggaatttt tttaaacaac ttcggtgcta gctcactatt
     3301 tcttgctcca gaatatttta acgaggttag tttttccagt acagccatag taggcttttc
     3361 tattggtgtt tttataatga gttggaatat cacaactttt attttacatg gcaaacacat
     3421 acagtttctt gcttctacag ctcaaccatt tctaaaatat tgtatcaaca atgcagtatt
     3481 accaatttta tttttaatat tttatttttt tagtgcctta cagtataata atcaaaaaga
     3541 gctaaaacca attgtagaaa tagctttact aagcagtggt tttttaatag gattcttttt
     3601 atccatcact atatcattta tgtatttttt tagtgccgat aaaagaatat ataagaacat
     3661 ggctacagta attcacgaag ccaataaaaa ctatgctatt gcatttacag aaaacccatt
     3721 accaaaagaa aaaagtcaaa taagaattga ttggtttttt agtgccagaa tgcacttacg
     3781 caaaccaaga gatataaggc attactcaac cgatttttta gatagaattt ttaagcaaca
     3841 ccatttagct gcaatacttg catttttctt agcttttata gcattaatag cattaggttt
     3901 ttcgtcagac tctagggtat ttcaaattcc tgctgcagct agtgtaacca ttttttttgc
     3961 tctaatcatt gcttttggtg gagcatttac attgtttctt aaatcctgga gtattccaat
     4021 aattattctt ttatatgttt tattaaatag tttgtacaag tacaatattt tagacttaag
     4081 aaacaaagct tatggtttga attatgaaaa taaaaacaac cgacctgaat atagtaaaga
     4141 aagtatttat gctttagcaa gcaaagaaaa tatagagaat gacaaactaa cttttttaaa
     4201 aagattatta cactggaaag ccaatcaaac agaagaccta cccatattat acatcattaa
     4261 tacaagtggt ggcggagtta gaagttctac ttttacattt agtgtgttgc aaatgttaga
     4321 ctctgttgta ttcaacggaa atttaatgaa aaaaactttt tttatcaatg gtgcatctgg
     4381 tggtatgtta ggtgctgctt attatagaga attgtatttt cgtaagcaac aaggctttaa
     4441 tataaatctt caaaacaaac aatatttaga tgatatttct aaagatttac taaaccccat
     4501 attctcttct tttgttagta gagatattat tggacctgtt caaaaattta aaaaatttgg
     4561 atatgaatat gtgaaagata gaggctatgc ttttgaaaga tctttaaacg ttaatacaag
     4621 aggttattta aataaaagat taatagatta tgccgaagtg gaaaacaaag caattatacc
     4681 taccatgttt tttaatagta ctattagcac cgatggtaga aaatttgtaa tgtgctccca
     4741 acctgttagg tttttaatga atcatgttaa tgcaaatgat ttatcttctg aacctgcacc
     4801 agatattatt gattttaata gcttttttag caagcaaaat agcaacaata ttggtatttt
     4861 aagtgcatta agaatgaatg ctacttttcc ttacgtttta cccaatgttt ggctgccaac
     4921 caatcctgtt attgatgtta tggatgcagg attaagagat aattttggac aagaaaacac
     4981 tttgcgtttt atagaagttt ataaaaattg gttaaaagaa aatacttcta aagtagttat
     5041 cattcaaata agagatagaa gtataaacga ctgggatgag gtggagaaaa ataaaagctt
     5101 atggagtgca tttaccaagc ctgcattctt attgcaatat aactggtata gactgcaaga
     5161 ttattaccaa catgcccagt tagagtatat gaatgaaagt tatggcagta atttacaaca
     5221 agtaagcttt caatatgttc catctaacaa aagcaaagct gcaagcttaa gttttcattt
     5281 aactactaac gaaaaaaaag aaatcttaaa ctctcttcat aataaagtaa atgtggatga
     5341 attaaccaag cttaaaaatt ttttagtaga aaaagaagat taattacaaa aaaaataccc
     5401 ttaaaaaaag ggtattaaaa aatgtaagca ataaataaat gctacttttt caatgcatct
     5461 ctaatttcaa ttaaaagctt gtcggtagat gaaggttctg ctggagctgc aggtgcctcc
     5521 tcttgttttt tcttcattct gttaatagct ttaattacca taaacattac aaaagcaaca
     5581 ataataaact caatagttac cgaaataaaa gaaccatact taatagctac ttcttctgta
     5641 cctgctactt tatctgcatc ttttaaaaca aacttccagt cagataagtc tttaccttgt
     5701 atcataccta ctaaaggcat caccatacca ttaataaaag ctcctgtaac ttttgtaaaa
     5761 gcagcaccca ttacaaagcc tattgctaaa tctataaggc tgcccttcat ggcaaactcc
     5821 ttaaattctt tcatcattcc cataattagt tagttttagt taataattat gtcaaattta
     5881 ggtttaagct aggaaaaatg gttagaaaga aatttatttt caaaaaaaat catctctagt
     5941 ttttattaaa aattcttacc ctaattacaa ctaattacca ttcatattat ttctttacca
     6001 ttgttatatt atttaacgtt aatacaacag ttaaacgatt attttcgtac ataagattat
     6061 tcccaatcaa ctctcccatt tatgaaaaaa attttatttg ctttcatttg tttaatgtta
     6121 attcaagtag ttcatgctca aaatagtact agccttaaag gaaaagtaac agatacactt
     6181 tcaaaacaaa atttaaaaga tgcatctata agcatgttaa acgcaaaaga ttccacagtt
     6241 gaatcttttg gccttgccaa aacagatgga acctttgaaa taaaaggctt tgctatgggc
     6301 tcttaccttt tacaaataag ttttaatggt tatgaatcct attttaaatc agttgttttt
     6361 aataaacaaa atccaacctt agatttaggt actattatct taaaaccaca agcaaaagag
     6421 ttagagggtg tttcggttgt tgctgctcct atacaagtta aaggagatac aacagaattt
     6481 aatgcaggaa gttttaaaac aaaacccaat gctacagcag aagatttatt aaaaaaatta
     6541 cctggtgtac aagtagaaaa agatggctct gttaaagcac aaggacaaac agtaaaacgt
     6601 gttttagtag atgggaaaag attttttggc gatgacccta ctactgcaac aagaaattta
     6661 cccaccgatg taattgataa agtacaggtt tatgatgcac aaagcgatca aagttctttt
     6721 agtggttttg atgatggcaa cagagataaa acaattaata ttattacaaa aaaagacagg
     6781 cgtaaaggct attttggaaa acttagtata ggtgcaggaa ataatgaacg gtatgcaact
     6841 aatgcaaatt ttaataggtt taatggcaat cagcaaattt catttattgg tcaaggaaat
     6901 aatattaacc aacaaaattt ttcaatgcag gatttatttg gcacaatgaa ctctgcagga
     6961 ggccgtggtg gtggtggaat gtttggaggt ggaatgagag gtggtaattt aggaaacttt
     7021 ttaacaacaa accaagcagg tattgctaca acatgggcag caggcttaaa ctacaacgat
     7081 gtttggagca aaaaaacaag tgtaagtggc agttacttct ataacaatat gaaaagaaat
     7141 aacgacaacg aaagccatac cgaaaccttt gtaactaacg attcttctca gttcaacaac
     7201 aacaacactt tttcaaatta tcttaatcaa aaccaccgat ttaattttga atttgaccat
     7261 aaattcgact ctgtaaattc attactggtt cgtcctaatt tttcttatca aattagcgat
     7321 aataaccaac aaaccatttc aactcttaca aagggaaagt tggttaattt aagcaatgta
     7381 caacaagtta caaatagcca aaacacaggt tacaacttta gtacaagcat tttactaagg
     7441 cataaattta aaaaacgtgg acgcactatt tctctaaacc ttacaccagg tttaagtaat
     7501 aacgaatcag acggtagcaa tatttcttat aacgatattt ataccataaa tggaatagtg
     7561 aaagacacca tcaatcaaat aaacagtaat gatagaaatg gtagaacttt tagtagtaac
     7621 ttatcttata cagagcccat tactaaaaaa agtcaattag agttaagcta taattacaat
     7681 tacagtaaaa acaattccaa tcaacaaact ttaatgtaca ataaacaaac taatagtttt
     7741 gatataattg tacccaacct taccaataag ttcgaaaaca ccaatacctc taataaattt
     7801 aatgctaatt atcgtgtaca actaactaaa gagtggagct acactttagg catgggtgta
     7861 cagtttgcag agcttacaag tataaataat actaaggcaa ccaagttaag caatacattc
     7921 actaattatt ttccatctgt acaattacac tatagtaaaa acagaactaa aaatttaaga
     7981 ttggtttata gggggcaaac caatgcacca tccataacac aactacaaga tgtaattgat
     8041 aatagcaacc aattaaatat aagaaatggt aatgcaggtt tagagcaaga gtttcagcac
     8101 aatttcaatt tattttatac caattttgat gtaattactt ttaaaaactt ctttgtaagt
     8161 attaacggaa gttttactca aaataaaatt ggcaatagtg ttattcaaaa tactggcaca
     8221 acaccaacag ttgtagatgg aatagttctt atacctggtg cacaatatac aaagcccatt
     8281 aacttaaatg gtgcaatgaa tatttttggt tttattaatt atggatttcc tataaaaaaa
     8341 cctaaatcta atttaaactt ttcaactact atgtcgtata taaaagatgt aaacctttac
     8401 aaccaaacta aaaattatac gcataattat attattggag aaagagttaa ttataatatg
     8461 aatgtaaaag aagtttttga tgtaaacttt gccagttctt cctcttacac ttttgccaga
     8521 tacactttaa ataacgagca aaatggcgat tattttactc aaacattttc tatagagcct
     8581 acctattcat caaaatcagg ttggattatt ggcagcgatt ttgatttaac tataaatcgt
     8641 ggtcaaagtg ttggttataa ccaaaccatc cctctatgga atgcctcaat tgcaaaaaca
     8701 atatttaaaa ataaaagtgg cgaaataaaa tttagtgtgt ttgatttact aaatcaaaac
     8761 aaaagtataa atagaactgt agagcaaaac tatattcaag ataccagaac gcaagtttta
     8821 acacgttact ttatgctatc atttacttat aacttaagaa aatttgctgg aaaagggcaa
     8881 caaatgcctc catttatgag aggtatgatg cgtaatgctc aaccaagaat gatgcgtatg
     8941 cattaatcat aaaatcaatt ttggtacttg ccaataatct ttactatttt gtgccaattt
     9001 aatttattaa gttggcatat ttattatata tagattccgt ttttgataca gcaaatattt
     9061 atttagcaaa taatgagcaa ataatttttg ccgataaaag tctcgaacaa aaaaaccatg
     9121 cttcatttat tcaagctgca attcaaaaaa actttcagaa aacccaaatt aatttacaat
     9181 caataaatgg cattgtagtt gtaaatggac caggaagtta tacaggcgta agagttggtt
     9241 tagcaacagc aaaaggcttg tgttatgcac taaatattcc attagtttta ttaaatactt
     9301 tagatataat ggcatttgaa atgaagagta acttatctaa atttattcct ctagctaact
     9361 ctacattacc aatattattt tgcccaatga ttgatgcccg aagaatggaa gtatttacag
     9421 ccctgtataa tcataattta aattatatta taaaaccttg tgcaagtgta ttaaacgaat
     9481 ttagttttga aaatgaacta aaagaaaata ctattttttt tagcggaaat gggagcaata
     9541 aattaaaaga agtgttaaaa aatcctaatg caatttttac tgatttacta tttcaagcat
     9601 ctacattaat tcaattgggg cttaatcaat ttaataaaaa taacttttct gacttagcat
     9661 atagccaacc tttttataca aaagaattct atacaactgc atccgccatt agtaaaatta
     9721 aaggtttaga taaataattt ttattggaaa aaaaaaaatt gtataaattt acatcctctt
     9781 taacccctaa ttttaataac aatgaaccaa acaaacattg tattgtctaa tggagagttg
     9841 tcgttacaaa ttgatcccct aagagtaaaa aagtctgcaa tggttattcg ggctctcaat
     9901 cataaacttc gtcagcaaat gattaagtta atagatgaag taaaaactat aaccgttaca
     9961 gatatttatg taaggcttag gctagagcaa tctgtagcat cacagcattt ggctgtttta
    10021 agaaaagttg gaattgtagc aaccaaaaga gatggtaaaa agatttttta ttccgtcaat
    10081 tatcatcgct tacaacacat attaaataca agtaacaact tattaaatta atcaattttt
    10141 aaaagatccg tatttactac tgatttttta ttactgctgt tcctcaacac agtcatcaat
    10201 aaactgtaat aattgtaatc ttccttcttt cttttcagta cttgtaataa aactttgtgg
    10261 taaaaactgc caggtttctc tcattttatt taaaaaaagc tgttcatttc tttttactac
    10321 aagtggtttt tctttatctg ctttagtaaa tactaataca aaaggaattt gccatttacc
    10381 taatttatct aaaaaatcta aatcattttt ttgagggctg tgcctactat ctattaatac
    10441 aaaaacattt atcaagttct ctctctttct catgtagtgt tcaatcattt gctcccatct
    10501 atttctatct ttttgagaac gtttggcata accataacca ggcaaatcaa ccaaatacca
    10561 actatcattt acaataaaat gattaattaa ttgggtttta ccaggtgtac cagatgtttt
    10621 agctaaattt ttctggttta ccaacatatt aattaaagac gattttccaa cattacttct
    10681 accaataaat gcaaattctg gcttatctgc aacagggcat ttatcaaacg taggcgatga
    10741 aataaggtat ttagcagttt taatttgcat aatgtaaaaa taaagttata tagcaaccag
    10801 ccaaatatct aatcttgttt tactaaatag gttagtaaat tgtataattg ccattaaaac
    10861 tttaattttg caccactttt cagtttttta aacattttaa aaatttaact catggtaaaa
    10921 gttgctatta atggttttgg acgaattggt cgtttagtat ttcgtcaaat ttataacatg
    10981 caaggtatag atgttgttgc aatcaacgat ttaacctctc ctgcagtgtt agcacattta
    11041 ttaaaatacg atagtgcaca agggcgtttt aatgcagagg tttctgctac agaagatgcc
    11101 attgttgtta atggagatac agtaaaaata tatgctcaaa aagatcctgc tcaaattcct
    11161 tggggaagcc atggagtaga tgttgtaatt gaatcaacag gcttctttac agataaagat
    11221 aaagcagcag cacacttaac agctggtgca aaacgtgtag taatttctgc tcctgcaaca
    11281 ggcgatttaa aaacagttgt atataatgta aaccatagca ttttagatgg ttcagaaact
    11341 atcattagct gtgcatcttg tactacaaat tgcttagctc caatggctaa agctttaaac
    11401 gataattttg gtttagtggc aggtagtatg accacaattc atgcttacac taacgaccaa
    11461 aacactttag atgctccaca tccaaaaggt gatttacgca gagcaagagc tgcagcacaa
    11521 aacattgtac caaacagcac tggtgcagct aaagcaattg gcttagtttt accagaatta
    11581 aaaggtaagt tagatggtgg tgcacaacgt gtacctacaa ttacaggttc attaaccgaa
    11641 ttagtaagtg tgttaaacaa aaaagtaact atagaggaag taaatgcagc aatgaaagca
    11701 gcaagcaacg aaagttttgg ttataccgaa gatgaaattg taagcactga tattataggt
    11761 ataaattttg gtagcttgtt tgatgcatca caaacaaaag ttattacaca aggcgataca
    11821 caaattgtaa aaactgttag ctggtacgat aatgaaatga gctatgttag ccaattagtt
    11881 cgtacagtaa aacattttgc tggtttaata agcaaataat ttttatgtta ccaatacaag
    11941 atatgctatt caacacctgt tgcgtcgcac acttgtactt taataattct attcaaaata
    12001 aaagttccga aaataaaatt tcggaacttt tgtatttata aataagcaat accttcacaa
    12061 aaaatttaac ccttcatttt ctaaaaatat tattatgagc aaattttcaa accaccaatt
    12121 cgcaaaccaa aaagcattaa ttcgtgtaga ttttaatgtg ccattagata aaacaacctt
    12181 agcaattaca gatgatacaa gaatgaaagc tgcaatacct accattcaaa aaatattaaa
    12241 cgatggtggc agtgtaattt taatgagcca tttaggaaga cccaaagaag gaccagaaga
    12301 taaatattca ttaaaacatt tagtacaaca cttaagtaat ttattaggcg gtaaaacagt
    12361 tttttttgca aatgattgta ttggtgaaca agcaacactt acagcatcaa tgcttaaagc
    12421 aggagaagtt ttattgttag aaaatttacg attttataaa gaagaagaaa aaggagatac
    12481 agcgtttgca gaaaaacttt caaaactagg agatgtttgg gtaaacgatg cttttggcac
    12541 tgcacataga gcacatgcat ctactgcaat tattgcaaaa ttctatgcaa aagaaaatcg
    12601 attttttggt ttattaatgg aggcagaagt aaaagcagca gaacaagtac ttcacaaagc
    12661 acaaaaacct ttcacagcta ttattggcgg agccaaagta tcggataaaa tattaattat
    12721 tgaaaacctt ttagaaaaag caacagatat aattattggt ggtggcatgg cttatacatt
    12781 tatgaaagct aaaggaggca gtattggagg ctctctttgc gaagacgaca gattgcaaac
    12841 tgctttagaa attttagaaa aagccaagca aaaaaatgta tctatacatt taccagaaga
    12901 ttcaataatt gcagacaagt ttgctgcaga tgcaaataca caattaacaa acagtaatgc
    12961 aataccaaca ggttggatgg gcttagatat tggcacaaat gcagcaacta aatttgccag
    13021 tgtaattaat agttctaaaa caattttatg gaatgggcca atgggtgttt ttgaaatgga
    13081 aaaatttcag gctggtacaa aagcaatagc tgttgcagta gcagaaagca cacaaaatgg
    13141 agcctttagc ttagttggtg gtggagatag tgtagctgct gtaaaccaat ttggatttaa
    13201 tgataaagtt agttatgtaa gtacaggcgg tggagccatg ttagaatatt ttgaaggtaa
    13261 aattttaccg ggtattgcag cagtgaatga ataacaaaac taaccaaatg caaacagatt
    13321 ttttagtaat tggtagcggt attgctgggt taacttttgc actaaaagtt gccaaccatt
    13381 ttccagataa aaaaattact gtaattacca aaaccaattc agacgaaacc aataccaaat
    13441 atgcccaagg tggtattgca ggtgttatag atgaaaccaa tgatagcttt gaaaaacata
    13501 ttgaagatac tttaattgct ggcgatggtt tatgcaataa agaaattgta gaaatagttg
    13561 taaaagaagg gccagctaga ataaaagaaa ttattaatta tggagcaaga tttgataaaa
    13621 atgaaaaagg agattttgct ttaggaaaag aaggagggca tagcgaattt agaattttgc
    13681 atcataaaga tgtaacaggt aaagaaatgg aacgtgcctt attacaagct gttactaata
    13741 caaaaaatat caacatcatt aatcattgtt ttgttgtgga tattattacg caacatcatt
    13801 taggttattt agttacaaaa tctactgtag atattacatg ttatggtgta tatgttttaa
    13861 acttaaacac caacttaata gaaaaaatag aaagtaaaat aactttatta gcaactggtg
    13921 gttgtgggca agcttataga gcaactacta accccaaaat tgctacaggc gatggcattg
    13981 caatggtata tagagctaaa ggtagaattg agaatatgga gtttattcaa tttcatccaa
    14041 cggcattata tgaacctggt atttctcctt cttttttaat tacagaagcc gttcgtggcg
    14101 atggtggcat tttacgcaac aaaaaaggag aagcctttat ggaaaaatat gatagccgta
    14161 aagaccttgc tccaagagat attgttgcta gggctataga caatgaaatg aaatttgaag
    14221 gcaccgaaca tgtgttttta gattgtactc acctacccaa agaaaaattt gttaaccact
    14281 ttccaaatat ttatcaaaaa tgtttaagca taggtattga tgttgccaag agctatatac
    14341 ctgttgcacc tgcagcacac tatagttgtg gtggcgtaaa aacaaatgca tttgcacaaa
    14401 cttctattaa aaatttatat gcatgtggcg agtgtgcaag cacaggctta catggtgcaa
    14461 atagacttgc tagcaatagc cttttagaag caatggtttt tgcacatagg gcatttgaaa
    14521 atattgttga aaatgcctct gaaatttttg aaagaaaaaa tattattata ataccagatt
    14581 ggaatacgaa aggtactatt gaacccagag aaatgatatt aatcacccag agttttaaag
    14641 agctacaaca aattatgagt gattatgttg gtattgtaag aacagatgta cgtttagcac
    14701 gtgctatgaa acgtttagac ctattatttg aagaaacaga acaattatat cgttccacaa
    14761 aagtatctcc acaactttgt gaactaagaa acctaataac tacaggttat ttaattgtaa
    14821 aaggtgccga ttttagaaag gaaagcagag gattacatta taataccgac catccttgta
    14881 aaagcgatat aatacaaaac actattttat aaaataacac tattctaaat tagttgttaa
    14941 ttcattactc tctgtggtag tagtattagg cattttcttt aattcttttc ctataatatt
    15001 agcagcacta aaacttgcta ccatgttatt aagcatctcg ccagcagcac ttggcgaatt
    15061 aggaagtaaa attaaattag aatgattatt agaacctatt gagtgtaatg tatcataatg
    15121 ttgtgtaacc acaattaaag cagaagcttc ttgcgaattg ataccaactt tgttaagaac
    15181 aataaccgat tcttctaggc cttttgcaat ttcccttctt tggtctgcaa taccttgccc
    15241 ttgtagtctc ttactttcag cttcagcctt ggctttttca acaattaaaa ttctttgagc
    15301 atcaccttga tattgggcag aaaccttgtc tctttctgct gcattaattc tgttcatagc
    15361 ttgctttact tcaacatcag ggtcaatatc ggtaaccaat gttttaataa tgtcgtaccc
    15421 ataagtattc attgcttctt gcaattcgcc tttaactgca attgctatat catctttttt
    15481 ctcaaaaaca tcatctaatc tcaacttagg aacctcagcc ctaaccacat caaatacata
    15541 agaagttatt tgcgacattg gattatctaa cttataaaaa gcttcaaaca ccttatcttt
    15601 aattacaaca tattgcaccg acactttcaa ttttacaaat acatcatctt ttgtttttgt
    15661 ttctataata acatctaact gttgaatttt taaactcaat cttcctgcaa tagtatcaat
    15721 aaatggaatt tttaactgaa agcctgcatt acgcacactg tgaaattttc ctaaccgttc
    15781 aactatttgt gcagtttgtt gctttacaat aaaaaacaac ccaaaaaaag ataaaataaa
    15841 aattaagatt actacataaa aaataatgtg ttccatatat tataagtttt atttttttaa
    15901 tgatagttaa agtgccaata tacaaaaatt aagctaataa aaaagtaaaa ttaattattg
    15961 agtttttagt tgcctactca aaaaaaataa aaattcttaa caaaaaatgt caaatactcc
    16021 acaatacaca atttcaattt ttttgcagca atgcattaca tacatttgcc catataattt
    16081 attttatggt tgatgttatt ttaggcttgc aatggggaga tgaaggcaag gggaaaattg
    16141 ttgatttttt tgctccacat tacgatgtta tagcacgttt tcaaggtggt ccaaatgctg
    16201 gtcatacttt atatgtagat ggtaaaaaag ttgtattaca tcaaatacct tctggcattt
    16261 ttcatcaaaa ctgtgttaac ctaattgcaa atggtgtagt acttgatcct gtcactttaa
    16321 aacgtgaatg tgagacagtt gcttcatttg gcattgatgt aaagaaaaat ttatttattt
    16381 cagaaaaaac aaatctaatt ataccaacgc atagagcctt agataaagca agtgaactac
    16441 aaaaaggaga aggaaaaatt ggtagcactt taaaaggtat tggccctgca tatatggata
    16501 aaacaggacg taatgcaata cgtgtaggtg atttattaag taaaaatttt acaacacaat
    16561 acataaaact tcgtttaaag caccaaaaat tattagataa ttttcatttt attgaagaca
    16621 ttactgcatg ggaagaggaa ttttttgaag ccatagattt tttaaaaact ttaaatattg
    16681 ttaacggaga atattttata aacaacaaat taaacgaagg aaaaaaagtt ttagcagaag
    16741 gggcacaagg tactatgtta gatatagatt ttggcacttt cccttatgtt acatcttcca
    16801 acacaattag tgcaggcgta tgtacaggct taggtgttag ccctcaaaaa attaatgatg
    16861 taattggtgt tactaaagca tattgtacaa gagtaggcag tggccctttt ccaactgaat
    16921 tgcatgatag tgttggcgat gaacttagaa aaattggtaa tgaatttgga gctacaacag
    16981 gtaggcctag gcgttgtggt tggatagatt tagtagcttt aaaatatacc tgtatgttaa
    17041 atggtgtaac caaagtagta atgactaaag ccgatgtgtt agatactttt gaaaatttac
    17101 aagtatgcac cagttacaca attgaaggaa aaaccactca agaaattcct taccaaatta
    17161 gcaaagtagc cattgaccct acctaccaaa cttttaaagg atggaactgc gattctacaa
    17221 aaattaattc tgcaaccaac ttaccagcta acatggtaac ttatattaaa tttattaatg
    17281 aatttattgg tgcacccgta aaatatgttt caaacgggcc aggaagagaa caaattatag
    17341 ttatttaatt gatataaaaa aatattagtt aatacagtct aaaattaatt ttgtttaaaa
    17401 ataaaataaa aaaaatagaa aaaaattaaa gaaaattttg gttagtaatt ttttgagttg
    17461 aaattttaca atccaatatt aaaacttatt agaactatgg cagcaaaatc tgataagggt
    17521 aaaaaatctg ttaagcccga aacaaaagaa attaaagaat caaaaaaaac ttcatcaaaa
    17581 cctaaaaaac aattagaaga ggaagatgaa gaggacaatt ttgaggatga tttagattct
    17641 ccatcaacct caaaaaaatc tagtaaaaaa actccttcca aaaaatcaaa ggaagaagat
    17701 gacgacgacg atgatgatga tgatgaggat aaagatgatt gggaaaaagt agaagaagaa
    17761 gaaaattggg atcctgattt tgatgaattt gatattccaa aagca
//
LOCUS       DAOQHP010000006        17648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1486_length_17648_cov_4.149093, whole genome shotgun sequence.
ACCESSION   DAOQHP010000006 DAOQHP010000000
VERSION     DAOQHP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1486_length_17648_cov_4.149093"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PLY81_00490"
     CDS             <1..1333
                     /locus_tag="PLY81_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HNF28736.1"
                     /translation="IVATIAFGMGIDKPDIRFVIHFNIPKSIENYYQETGRAGRDGLE
                     GICVLYYSHKDVSKLEHLMRDKPLSEREVGAQLINETVAFAESSVCRRKILLHYFGEE
                     WGDRKCGNCDNCRNPKEKIEAKEDVVKALKVIEALDERFVADYTINVIMGKLTPQITM
                     FRHDKSPVFGIGKDKDTLFWNSLLRQMMLENLITKDIEEYGLIKFTEKGLEWKKKPSS
                     FKIVINNIFHDTDDEDENIEMGEGTGAGADDALFEMLKELRHKEAKKKGLPPFVIFLE
                     NSLQDMSTLYPTTLDELEKCQGVSKGKALKYGRPFVELIAKYVEENDIEKPDDFVMKS
                     VANKSSNKIFIIQNVDKKIPLETIASIKGLTLNELLEEMETIAASGTRLNLDYAIDDW
                     LDEIDQEEILEYFKSCETSSLEIAQKELAEYNYNWEQLKIMRIKFLSVYGN"
     gene            1378..2439
                     /locus_tag="PLY81_00495"
     CDS             1378..2439
                     /locus_tag="PLY81_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28737.1"
                     /translation="MKFIYISKYLITTLMVVTFFIVGCKPNKNIPDVSSIKIDLSTER
                     FEKDFFNLDTLHLDSSIQKLFDKYPDFSVNYLHNILAAAPHADSVINTILHFTKDYKK
                     LYNDAENLFADFSPYEKEIKQGFQFVKYYFPNYTLPTKLVTYIGTWDAIIMLSDNSGG
                     SGCIRMSEDVLGIGLQLSMGTNYAMYKDEMMQQFYPEFISRRFDKAFIGVNALKVIVD
                     DIYPMQNKSYTLIEQMIEAGKRLYVLNAFMPTTPDSLKTGYTQQQLEGCYKNEANIWS
                     FFITNSLLYETQPSIVMEYMNDAPRTAALGDAAPGLIGKFVGWQIVKKWMEKNNKKTL
                     QDLLSTAPKNIFEQAKYKP"
     gene            2446..5484
                     /locus_tag="PLY81_00500"
     CDS             2446..5484
                     /locus_tag="PLY81_00500"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF28738.1"
                     /translation="MIVVKHCIFFFICFYAITAKAQIPTYIHYEVNDGLPSNEIYSLK
                     QDKNGFLWIGTEGGLAKYNGRNFILYPHKNLKGLGITGIQEDAFGKVWCYNFSGQILH
                     TQNDSLVVFKPWANFYKEQFADFALDNNNQIFVSNFKNNVYVFNIKYLNLVNSLSNNT
                     TIKQGISVTHDNKIIFTNLTDGKPYIIEQNLLIPVPLKAWQNLPADAKAENTLVFFKS
                     SKNLQTFAIQRLHPNIPFPSLYQFVHNQLQIHPVTAYLRKNKIHPLSVLNDDEGNLFI
                     GTFNGCLWFKQQGENWNFHKNIFENNAVSAIIKDRENSYWFATLKNGLYQVPDLAFQI
                     FTKEDIGILETEIGDVETDGKSKLFVSGSTNELFTINILNNKLVHKNFTKDKRYAQAF
                     YYDKLFNRLLLYKSEVSILENNILKNISTLTSAAKNFYVRKDGVIFIAGSSLDAIFLT
                     SNKNLTLTSEFGEVEKSNTELNKHNSNIWGRVVISTQRSRDVWYNEKDKILWSANSDG
                     VVYYKNKKVNTLFDTKSNKQIIATCFQNLENNLLCIGTVDQGIYIIQDTSIVLHYTTK
                     NGLQSNQINKIKADEQNIWAITDKGIQRFNLSTNNFINISKSDGLISNQVFDMAIANN
                     TVYISTSKGLQFLPVNAIKTSNQLPFLGIKKVSINDSIYNYKNTILLNSKNGNIEIEL
                     EYALLKNSGNIALKYRMIGLDTLWIEDKAFNNIIRYNTLSSGSYKFEAVCMNDDGVES
                     KPVTIHFTVLKPWWLQWWFLITAACISILLVIFFTKLYLNISKNKIAEELKKSKLQEE
                     ARQSQLASLKAQMNPHFMFNALNSIQEYILLSDKRLANMYLGKFADLMRLILDQSNKE
                     IISLVEELKILNLYLELEALRFEENFEYIIQKENIEDEAEINIPAMLIQPYVENAIKH
                     GLLHKQGLKKISIHFKLQKNKLLCVVEDNGIGRKRSGEINALRNKKYTSFATGATQKR
                     LELLNQNNPNLIVVNYTDLTLNNTTYIGTKVSIVIPIIEL"
     gene            5577..7418
                     /locus_tag="PLY81_00505"
     CDS             5577..7418
                     /locus_tag="PLY81_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF28739.1"
                     /translation="MTQKFTLVFTIFLFTTISLMAQKPIVSFSFDNTLNDTTNTFAFT
                     PSTALTGSGWGFATNRFGEANKAFACTNNSNRSLTANITSLPQGNSARTIEFWYKFNS
                     SSSPNFLVAYGSNITSQGYGITQESNKIINYGWANDIEVAQTFTVGVWYHYAIVYDGV
                     TAYIYRNGLFLTSAAKYWNTIGTNLLIGSTPSLLKSFNGTIDDLRIYDVELGLPSIQY
                     LYNNAKNLPSPPYKWNFNNSYSDTTNQMSFSSNAGTSFVNDRFGNANSAININNTGCT
                     ATIFGLPYHKKPRSISFWVKHNVVIGSPMFNMWYSYGKNGTNYANGGGYHQLDGIYHF
                     GYLNNHATTISSPLLPNQWYHIVFVFDGTLSKIYSNGTLLSSQTMTGWNTLGIDDVFK
                     LGIGIGHEISFNGAIDDLRIMNKALTNAEVSALYASESTLPMQLHQFSAQLKNNTTLL
                     KWSTATEVNTSHFEIEYSNNGKEFTTVGTVAAKGNSSNLNAYNFTHNVNNQSMHYYRL
                     KMIDNDAKFEYSNIVKLKTTNKFFDAIVYPTIANNTITASITAKEKANATIELFGMNG
                     NKVLQKTVSFVEGSQTYNINIAHLIKGSYIVSIKAGNNVQSSRIIIQ"
     gene            7572..8315
                     /locus_tag="PLY81_00510"
     CDS             7572..8315
                     /locus_tag="PLY81_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF28740.1"
                     /translation="MLKAIIVDDEAKARRILENFITEYCPQIQICALVEDVPQAVKAI
                     KKHEPDVVFLDIEMPGYNGFQLFEFFDEVNFEIIFITAYSEFALKAFQVSAIDYLLKP
                     LQIDQLINAVKKLETKANKATLKNRVEALQKNIQEQVIKKIVVPFSNGSLFLETSEIT
                     HLKAEGSYVNIFMKDGSKILVSKNIKDYEEILTANEGFFRTHRSFLLNKNYIVKLNSD
                     STEAVLTTGNSILIARERKQDFLQFIKNR"
     gene            complement(8319..10037)
                     /locus_tag="PLY81_00515"
     CDS             complement(8319..10037)
                     /locus_tag="PLY81_00515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNF28741.1"
                     /translation="MPLNINIKKLSRNFLPLEFTVTTWDKLEPYFKNLVDRIINSKED
                     LEQWLLDLSELEAVVSEDACWRQIKMTCDTTDKSLEEAFTFFCMEIQPKIQPYADLLN
                     KKLIHNQFTKQLDQTKYFTYLRGVKKNIDLFREANIPIQAELSVLQQQYGAIAGKMTV
                     EFEGKEYTLQQASKFLESKDRSVRETMYKKIQERRLQDANELQNLFTILISKRNTIAI
                     NAGFKNYRDFKFVDLGRFDYTKEDCYQFHEAVKQHILPLVNKIYAKKQAQLGLTTLQP
                     WDTEAEPIGVEPLKPFKTAKDLYEKTVQCFEQLDPFFANCLRQMNELKHFDLESRKGK
                     APGGYNCPLAESGAPFIFMNATGQMSDVTTMVHEGGHAIHSFLSHHLELCAFKEYPME
                     IAEVASMAMELFSMDYWHSFFDNEAELKRAKEHQLERTITIFPWIATIDKFQHWIYEN
                     PTHSEEERTNKWMEIVQEFSSDVIDFTGLDEYRKISWQKQLHLFEVPFYYIEYGIAQL
                     GAIGLWMQFKQNKKQALNNYTNALSLGGTKTLPELYNAAGLQFNFTPAYIQQLMDFVS
                     NEMEHL"
     gene            10361..10978
                     /locus_tag="PLY81_00520"
     CDS             10361..10978
                     /locus_tag="PLY81_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF28742.1"
                     /translation="MFEIILENTGKRFNKEWIFKDLHYVFKSNNFYAIEGANGSGKST
                     LLQIIAGSMLASNGNVKYKTNSTEIASEKVYHHVAIAAPYIEVIEEMTANEFLHFHHS
                     FKQLVLPVPEILQIIALEKAANKQIRYFSSGMKQRIKLAQAFFSNTPVLLLDEPCTNF
                     DAAGFNLYENLLTNYCSNKLVIICSNDENEIKQCHYRLNMLNFKT"
     gene            11222..11632
                     /locus_tag="PLY81_00525"
     CDS             11222..11632
                     /locus_tag="PLY81_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF28743.1"
                     /translation="MAKVAVYRKEHFNAAHRLHNNNWSEEQNKLIFGKCNNENYHGHN
                     YELVVKVIGEPNIDTGFVIDTKELSNLIQKEVIEKFDHKNLNKDIAAFKNIIPTAENI
                     AIEIYNILRPQIETKLELQIRLYETERNFVEYPS"
     gene            11595..12317
                     /gene="folE"
                     /locus_tag="PLY81_00530"
     CDS             11595..12317
                     /gene="folE"
                     /locus_tag="PLY81_00530"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNF28744.1"
                     /translation="MKQKETLLNIRLNAENKIELSDMMIDEMGNDHVSTSVDTPIRED
                     AFELSDSEKVKEIEKHFTSIMQILGLDLTDDSLKGTPKRVAKMYVQEIFNGLNPANKP
                     SITLFENKYGYNEMLVEKNISFYTCCEHHFVPFNGKAHIAYINSGKVIGLSKLNRIVK
                     YFAKRPQVQERLTMQIGKELQKVLNTLDVAVLIDAKHLCVASRGVEDDTSSTVTAFYG
                     GAFNEEDKKTEFLKYLNLETKY"
     gene            12388..13083
                     /locus_tag="PLY81_00535"
     CDS             12388..13083
                     /locus_tag="PLY81_00535"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNF28745.1"
                     /translation="MQKKKLVVFTGAGISAESGLKTFRSNNGLWMNYKIEEVATPRGF
                     AANPKLVLDFYNMRRREVAAAKPNNAHLQLVDLEKLFDVTIITQNIDDLHERAGSKHV
                     VHLHGHIFEMKSTQNSYKIYKTNGDIHVGDLAEDGFQLRPNIVWFEEPVPMINIAEEI
                     AATADIFVVIGTSLQVYPAAGLINCTRAYIPKFIIDNDIPKVAKHINQLTVIEKTASE
                     GVADLVKILNEIK"
     gene            complement(13089..13601)
                     /locus_tag="PLY81_00540"
     CDS             complement(13089..13601)
                     /locus_tag="PLY81_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNF28746.1"
                     /translation="MNKWMKYKIIFVFSCLLFCTIVKANNTHKYIGKYKDLVEELSKE
                     FGIPRSIITAVSIIESGAGRSRNCKLLKNHFGIIGRNNLLKTKGIKTRFKQYETDEDS
                     FRDFCKLLSRRKYYKKLKGNNNYTLWINAIAGAGYSTTPNVWKKEITRTIKYYKLDKL
                     DEEVKEVNED"
     gene            13710..14570
                     /locus_tag="PLY81_00545"
     CDS             13710..14570
                     /locus_tag="PLY81_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF28747.1"
                     /translation="MKRFFVFISFLLFLVACKKNITDNSASVAALTLTDVAYASANSN
                     QKLDVYLPANRSTATTYTCIMVHGGSWSGGDKSDFNTDIATLKTLVGNYAIININYSL
                     ANGSSILLQQQIDDLNAAFNFIHSKANEYLINTNKIAIIGASAGAHLSLLKAYKYNTD
                     NKIKAMVDLFGPTNMTWMYTSHPYPTIAQPTIVNVMGATPITNAAAYYNGSPINFVSS
                     TVPPTIIFHGTADEIVPISESVNLKNALATAGVQYEYYTYTGEAHGWTGANLTDTYNK
                     VITFIKNYVK"
     gene            complement(14587..15309)
                     /locus_tag="PLY81_00550"
     CDS             complement(14587..15309)
                     /locus_tag="PLY81_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNF28748.1"
                     /translation="MKKFIAILVVTIFTSNIQAQVIKGLINKVTNKDNNAKSGLSTEE
                     VVQGLKEALTVGAEKSANQLSNVDGFFKNEAIKILLPPEAKKVQDALSKIPGGSKYVE
                     DAILSMNRAAEDAAKSAAPIFINAIKNMSFADAWEILKGSDSAATKYLKQQTSKPLTD
                     AFRPIIDKSLAKVNATQHWNTVFSTYNKFSFKKINPDLASYVTEKALEGIFYQLAQEE
                     LKIRKDPIAQTTNLLKKVFGGK"
     gene            15489..17429
                     /gene="dnaG"
                     /locus_tag="PLY81_00555"
     CDS             15489..17429
                     /gene="dnaG"
                     /locus_tag="PLY81_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNF28749.1"
                     /translation="MITPQTIEQIKSRIDIIDIISQYVNLKKRGSNYIGNCPFHNEKT
                     PSFTVSPTKELYKCFGCGKSGNAITFLMEHDKLSFVEALKWLAAKYNIEIEETETTAE
                     QKIVTQTADSLYILNNFATNFLEKELWQGEEGVAIALTYLKERGFREDIIKKFQLGYN
                     PTQKDKLVTTLLNNQFNKELIPKSGLGVERNQQELVDNYRGRIIFPIHNNTGKIIGFG
                     ARVIGNAKDKAPKYINTPENEIYIKSKILYGIYFARSPIDKANECLLVEGYTDVISLH
                     QAGIENVVASGGTSLTTEQIRIIKKFTSNLTIIYDGDSSGIKAALRGLDMALEEGLNV
                     KLVLIPDNEDPDSFVNKVGTTAFKEFIKNNKKDFIIFQLEVILKDAGNDIVLKNNAVN
                     NIADTLSKINRPEDFTKLQEYIKQCAAILKIDELGLTNLVNKFKADKLAKDAKKQANN
                     TTFQQQEFPEQTINTSNDDVSILFNQDDESERNLLKVLLEHGLKQFNQEQTIAEFIFN
                     ELNTFYFQNNLIETIVEEYRTAYNEGKQPEPKTFLYHTNNEIRNLVIAITATPYELSV
                     RWDDKLQGMNIVNRDTSTQDALISVNFFKLRKIKKMIDENQHDLEVETDLRKQEYYIT
                     VHKELKETEKNITQTLGTVILK"
     gene            complement(17443..>17648)
                     /locus_tag="PLY81_00560"
     CDS             complement(17443..>17648)
                     /locus_tag="PLY81_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28750.1"
                     /translation="NMAYFFLAKANENLQCQQPDPFEDLDVILVDKKEVLEMIHNSQI
                     IHGVQIGAIYAAYQKLGWIEQKI"
BASE COUNT         6590 a         2432 c         2735 g         5891 t
ORIGIN      
        1 aattgtggca acaatagctt ttggaatggg tattgataag ccagacatta ggtttgtaat
       61 tcatttcaat attccaaaat ctatagaaaa ttattatcaa gaaacaggac gtgcaggcag
      121 agatggttta gaaggaattt gtgtgctgta ttattctcat aaagatgttt caaaactaga
      181 acacctaatg agagataaac ctttaagtga aagagaagta ggggcacagc taattaatga
      241 aacagttgct tttgctgaaa gcagtgtttg ccgacgaaaa attttattac attattttgg
      301 cgaagaatgg ggggatagaa aatgtggcaa ctgcgataat tgtagaaacc ctaaagaaaa
      361 aattgaagca aaagaagatg tagtaaaagc attaaaagta atagaagctt tagatgaaag
      421 atttgttgct gattacacca tcaatgtaat tatgggtaaa ttaactccgc aaattactat
      481 gtttagacac gacaaatctc ctgtatttgg aattggaaaa gataaagaca ctttgttttg
      541 gaatagtttg ttaaggcaga tgatgttaga aaaccttatt acaaaagata ttgaagaata
      601 cggattaata aagtttacag aaaaaggact agagtggaag aaaaaaccca gctcctttaa
      661 aattgtaata aacaatatat ttcatgatac agacgatgag gatgaaaata ttgagatggg
      721 agaaggaact ggtgcagggg cagatgatgc tttattcgaa atgcttaaag aacttagaca
      781 taaagaagca aaaaagaaag ggctgccacc ttttgtaatt ttcttagaaa actccttaca
      841 agatatgagt accttgtacc caactacttt agatgagtta gaaaaatgcc aaggggttag
      901 taaaggaaaa gcattaaaat atggaaggcc atttgtagaa ttaatagcaa aatatgtaga
      961 agaaaatgat atagaaaaac cagatgattt tgtgatgaaa agtgttgcca acaaaagcag
     1021 caataaaatt tttattatac aaaatgtaga taaaaaaatt ccgttagaaa ctattgcaag
     1081 tattaaaggg cttacactta atgaactttt agaagaaatg gaaactattg cagcaagtgg
     1141 cacaaggctc aatttagatt atgctataga tgattggtta gatgaaattg accaggaaga
     1201 aattttagaa tactttaaaa gttgcgaaac atcgagcctt gaaatagctc aaaaagaatt
     1261 agcagaatat aattataatt gggagcaact taaaataatg cgaattaaat ttttaagtgt
     1321 gtatggaaat taatggttaa aacttccttt ttatatactt taaaaaaatt acaagttttg
     1381 aaatttattt atatttctaa atatttaatt acaaccttaa tggttgtaac tttttttatt
     1441 gttgggtgta aacccaacaa aaatattcct gatgtaagta gtattaaaat tgatttatct
     1501 accgaaagat ttgaaaaaga cttttttaat ttagatacgc tacatttaga tagctctatt
     1561 caaaaactat tcgataagta tcctgacttt tctgtaaatt atttacacaa tattttagca
     1621 gctgcaccac atgcagatag tgtgattaat accatattac attttacaaa agattataaa
     1681 aaactgtata atgatgctga aaatttattt gctgattttt ctccttatga aaaagaaatt
     1741 aagcaaggat ttcaatttgt aaaatattat tttcccaatt atacattacc cactaaactg
     1801 gttacatata ttggcacctg ggatgctatt ataatgctaa gtgataatag tggtggctct
     1861 ggttgtataa gaatgagtga agatgttttg ggtattggct tacagttaag catgggtact
     1921 aattatgcaa tgtataaaga tgaaatgatg caacagtttt accctgaatt tataagtaga
     1981 agatttgaca aagcttttat tggcgttaat gctttaaaag taattgtaga tgatatttac
     2041 cccatgcaaa acaagagcta cactttaata gaacaaatga ttgaggcagg caaaagattg
     2101 tatgtattaa atgcatttat gcctaccaca cccgatagtt taaaaactgg ttatacccag
     2161 caacaattag aaggttgtta taaaaacgaa gccaacattt ggagcttttt tataactaat
     2221 agtttgttat acgaaacgca accatctatt gtaatggagt atatgaatga tgcaccaaga
     2281 acagcagcat tgggagatgc tgcacctggt ttaataggaa aatttgtagg ctggcaaatt
     2341 gtaaaaaaat ggatggaaaa aaataataaa aaaacattgc aagatttatt gagtactgcc
     2401 ccgaaaaata tttttgaaca agcaaaatat aaaccataat attttgtgat tgttgtaaag
     2461 cattgtatat ttttttttat ttgcttttat gctataacag ctaaagcaca aatacctacc
     2521 tatatacatt atgaagtaaa tgatggctta ccatccaatg aaatttatag tttaaagcaa
     2581 gacaaaaatg gctttttatg gataggtaca gaaggtggtt tagcaaaata taatggaaga
     2641 aattttatct tatatcctca caaaaattta aaaggcttag gtataacagg tattcaagaa
     2701 gatgcttttg gaaaagtgtg gtgttataat ttttctgggc aaatattgca tacacaaaac
     2761 gattctttag ttgtatttaa accttgggca aatttttata aagagcagtt tgcagatttt
     2821 gctttagata ataataacca aattttcgtt tctaatttca agaataatgt gtatgttttt
     2881 aatattaaat atttaaacct agtaaatagc ttatcaaata atactaccat aaaacaaggt
     2941 attagtgtta cacatgataa taaaattatt tttaccaatc ttaccgatgg aaagccatat
     3001 ataattgaac agaatttatt aattcctgtt ccactaaagg cttggcaaaa cctaccagcg
     3061 gatgctaagg ctgagaatac tctggtgttt ttcaaatcat ccaaaaacct tcaaaccttt
     3121 gctattcaaa ggctacatcc taacatcccc ttcccttctt tatatcaatt tgtacataat
     3181 caacttcaaa ttcatcctgt tactgcctat ctaagaaaaa ataaaattca tcctttaagt
     3241 gtattaaatg acgacgaggg aaatttattt attggaacat ttaatggttg tttatggttt
     3301 aaacaacaag gcgaaaactg gaattttcat aaaaatattt tcgagaacaa tgcagtatct
     3361 gccattataa aagacagaga aaatagttat tggtttgcta cattaaaaaa tggattatat
     3421 caggttcctg atttggcatt tcaaatattt acaaaagaag atattggaat tttagaaacc
     3481 gaaattggcg atgtagaaac tgatggcaag agtaaattat ttgtatcagg atctaccaat
     3541 gaactattta ccatcaacat cttaaataac aagttagttc ataaaaattt cactaaagat
     3601 aaaaggtatg cacaggcatt ttattacgat aagttattta ataggttgct tttatataaa
     3661 tcggaagttt caattttaga aaataatatt cttaaaaata tcagcacttt aaccagtgct
     3721 gctaaaaact tttatgtaag aaaagatggt gtaattttta ttgcaggctc ttccttagat
     3781 gcaatatttt taacctcaaa taaaaattta accctaacaa gtgagtttgg agaagttgaa
     3841 aaaagcaata cagaattaaa caaacataat agcaatattt ggggcagagt agttatttca
     3901 acacaaagat ctagagatgt ttggtacaat gaaaaagata aaattttatg gtcggcaaat
     3961 tcagatggag ttgtttatta taaaaataaa aaagtaaata cattatttga tactaaatca
     4021 aataaacaaa ttattgctac ttgttttcaa aatttagaga ataatttatt atgcattggc
     4081 actgtggacc aaggtattta tataatacaa gacacctcca ttgttttaca ttatacaact
     4141 aaaaatgggt tgcagtctaa ccaaattaat aaaataaaag cggatgaaca aaatatctgg
     4201 gcaattacag ataaaggcat tcaacgattt aatctttcta caaacaattt tatcaatata
     4261 tcaaaatcag atgggcttat ttcaaaccaa gtttttgata tggcaattgc caataataca
     4321 gtttatatat ctacctctaa aggcctacaa tttttacctg ttaatgcaat taaaacaagt
     4381 aatcaattgc catttttagg aattaaaaaa gtaagcatta atgatagtat ttataattat
     4441 aagaacacta tactactaaa tagtaaaaat ggaaatattg aaattgaatt agaatatgca
     4501 ttattaaaaa actctggaaa tattgcactt aaatatagga tgattggttt agatacttta
     4561 tggatagaag ataaagcatt taacaatatt atcaggtaca acactttatc ttctggaagt
     4621 tacaaatttg aagcagtttg tatgaatgac gatggggttg aaagtaaacc agtaacaata
     4681 cattttactg ttctaaagcc ttggtggtta caatggtggt ttttaattac tgctgcatgt
     4741 atttctatcc tgttggtaat atttttcaca aaattatatc ttaatatttc taaaaataaa
     4801 attgccgaag aactaaaaaa atcaaagttg caggaagaag ccaggcaatc gcagttggca
     4861 tccttaaaag ctcaaatgaa cccccatttt atgtttaatg cattaaattc aattcaagaa
     4921 tatattttgc ttagcgataa aaggttagcc aatatgtact tgggcaagtt tgcagattta
     4981 atgcgattga ttttagacca aagcaataaa gaaattattt ctttagtgga agaattgaaa
     5041 attttaaacc tttatttaga attagaagca ttgcgttttg aggaaaattt tgaatacatt
     5101 attcagaaag aaaatattga agatgaagct gaaattaata ttcctgcaat gctaatacag
     5161 ccttatgttg aaaatgcaat aaaacatggg ttattacata agcaagggtt gaaaaaaata
     5221 agtatccatt ttaaattgca aaaaaataaa ctactatgtg ttgttgagga taatggtatt
     5281 ggtagaaaac ggtcaggaga aattaatgcg ttaagaaata aaaaatatac ttcttttgca
     5341 actggtgcca cacaaaaaag attagaactt cttaaccaaa ataatccaaa tttaattgta
     5401 gttaattata ccgatttaac attaaacaat acaacctata ttggtactaa agtgagtatt
     5461 gtaataccaa ttattgaatt ataattacag tttacaaaac aatttataca gcccacaaag
     5521 tttagttcat tgtttaaata agtcaataaa catttgtgtt aataatttaa taatttatga
     5581 cacaaaaatt tacacttgta tttactattt ttctctttac aactatttcg ttaatggcac
     5641 aaaagccaat tgttagcttt tcatttgata atacattaaa tgacacaact aatacctttg
     5701 catttacacc tagtactgct ttaacaggta gtggttgggg ctttgcaaca aataggtttg
     5761 gagaagccaa taaggctttt gcatgtacta ataactccaa tagatcttta acggctaata
     5821 ttacttcatt gccacaagga aattcggcaa gaacaataga gttttggtat aagtttaact
     5881 caagttcttc cccaaatttt ttggtagctt atggtagtaa tattactagt cagggttatg
     5941 gaattactca agaaagtaat aaaataatta attacggttg ggcaaatgat attgaagttg
     6001 ctcaaacctt tactgtaggt gtttggtacc actatgcaat agtatatgat ggtgtaactg
     6061 cttatattta tagaaatgga ttgttcttaa ccagtgctgc aaaatattgg aatacaattg
     6121 gcactaattt attaattggc tctaccccaa gcttattaaa gagctttaat ggtacaatag
     6181 atgatttaag aatttatgat gtagaattag gacttccttc tattcaatac ttatacaaca
     6241 atgcaaaaaa tttaccatca ccgccatata aatggaattt taataattct tatagcgata
     6301 caactaacca aatgtctttt tcttcaaatg caggaaccag ttttgtaaac gataggtttg
     6361 gtaatgcaaa tagtgcaata aatattaata atacaggttg tactgcaaca atatttggtt
     6421 taccatatca taaaaaacca aggtctatct ctttttgggt taaacataat gttgtaatag
     6481 gcagtcctat gtttaatatg tggtatagtt atggaaaaaa tggaacaaat tatgcaaatg
     6541 gaggtggata tcaccaacta gatggcattt atcattttgg atacttaaat aatcatgcaa
     6601 caacaatatc aagcccttta ttgcccaacc agtggtatca tattgttttt gtttttgatg
     6661 gcactttgtc aaaaatctat agcaatggta cacttttgtc atcgcaaaca atgacaggtt
     6721 ggaatacact agggattgat gatgtattta aattaggtat aggtataggt catgaaatat
     6781 cttttaatgg agctatagat gatttaagga taatgaacaa agcattaaca aatgcagaag
     6841 taagtgcatt atatgctagt gaaagtacct tgcccatgca gttacatcaa ttctctgctc
     6901 aactaaaaaa caatactaca ttattaaaat ggagcactgc taccgaagta aatactagcc
     6961 attttgaaat tgaatatagt aataatggga aagagtttac tacagtagga actgttgcag
     7021 caaaaggcaa tagttctaac ttaaatgcat acaattttac acacaatgta aacaatcaat
     7081 ccatgcacta ctacaggtta aaaatgatag ataatgatgc aaaatttgaa tacagtaata
     7141 tagtaaaatt aaaaactacc aataaatttt ttgatgcaat agtttatcca acaatagcaa
     7201 ataatacaat tacagcaagc attactgcta aagaaaaagc aaatgcaact atagaactgt
     7261 ttggaatgaa tgggaataaa gtattacaaa aaactgtttc atttgttgaa ggaagtcaaa
     7321 catacaacat caatattgca catcttataa aaggtagtta tatagtaagc attaaagctg
     7381 gtaacaatgt acaatctagc agaataatta tacaataata aataataacc atcttggcaa
     7441 acaaggaatg cttgaacaat aaattggggg atttattcat tcaggcgttc caatattgcc
     7501 tttaattttt tgaaatgcaa aacaagtttt ttaatatata ttaaacttaa tttgtaaaaa
     7561 attaatgcat aatgctaaaa gcaataattg tagatgatga ggctaaagct agaagaattt
     7621 tagaaaattt tattacagag tattgcccac aaatacaaat ttgtgcatta gtagaagatg
     7681 tgcctcaagc tgtaaaagca attaaaaaac acgaaccaga tgtagtgttt ttagatattg
     7741 aaatgcctgg ttacaatggg tttcagctgt ttgaattttt tgatgaagta aattttgaaa
     7801 ttatttttat caccgcttat agcgaatttg ctttaaaagc ttttcaggtt tcggctatag
     7861 attatttatt aaaaccatta caaatagatc agttaataaa tgctgtaaaa aaattagaaa
     7921 ccaaagccaa taaagccact ttaaaaaaca gagtggaagc attacaaaaa aatatacaag
     7981 agcaggtaat taaaaaaatt gtagttcctt ttagcaatgg cagtttgttt ttagaaacga
     8041 gtgaaattac tcatttaaaa gcagaaggta gctatgtaaa tattttcatg aaagatggca
     8101 gtaaaatatt agtatcaaaa aatataaaag attacgaaga gatattaact gcaaacgaag
     8161 gtttctttag aacacacagg agttttttac taaataaaaa ttatatagta aaactaaact
     8221 cagactctac agaagctgtt ctaactacag gaaacagcat tttaattgcc cgagaaagaa
     8281 agcaagattt tttacagttt ataaaaaata gataacattt ataaatgctc catttcgtta
     8341 cttacgaaat ccattagttg ttgtatataa gcaggtgtaa agttaaattg taaacctgct
     8401 gcattatata actcaggtaa agtttttgtg ccccctaagc ttaatgcatt ggtataatta
     8461 tttaatgctt gttttttatt ttgtttaaac tgcatccaca aaccaatagc acctaattgt
     8521 gctatgccat actcaatata ataaaaaggc acttcgaata aatgcaattg tttttgccaa
     8581 cttatttttc tatattcatc taaacctgta aagtctatta catccgatga aaattcttgt
     8641 acaatttcca tccatttgtt agttctctcc tcttctgaat gtgttgggtt ttcgtaaatc
     8701 caatgttgaa atttatcaat cgttgctatc cacggaaaaa tagttatagt tctttctagc
     8761 tgatgttctt tagctctttt taattcagct tcattatcaa aaaagctatg ccaataatcc
     8821 atgctaaaaa gctccattgc catactagca acttctgcaa tttccattgg gtattcttta
     8881 aaagcacata attctaaatg gtggcttaaa aaagaatgaa tggcatgccc accttcgtgt
     8941 accattgtag ttacatcact catttgccca gtagcattca taaaaataaa tggtgctcca
     9001 ctttctgcca atgggcagtt ataaccacct ggagcttttc cttttctact ttctaaatca
     9061 aaatgtttaa gctcattcat ttggcgtaaa caattagcaa aaaaaggatc aagttgttca
     9121 aaacactgta ctgttttttc gtacaagtcc ttagctgttt taaatggttt taatggttct
     9181 acgcctattg gctcagcttc tgtatcccat ggttgtagag ttgttaaacc aagctgtgcc
     9241 tgtttttttg cataaatctt attcaccaaa ggcaaaatat gttgtttcac tgcttcgtga
     9301 aattggtagc agtcttcttt agtataatca aatcttccaa gatctacaaa tttaaaatct
     9361 ctataatttt taaaaccagc attaattgca attgtattcc tttttgaaat aagaatggta
     9421 aataaatttt gcaactcatt agcatcttgc aaacgacgtt cctgaatttt tttatacatc
     9481 gtttctctta cgcttctgtc tttactttct aagaacttag atgcttgttg caaggtatat
     9541 tctttaccct caaactctac agtcattttg cctgcaatag ctccatattg ttgttgcaaa
     9601 acacttaatt ctgcctgaat aggaatattt gcttctctaa ataaatctat atttttttta
     9661 accccacgta agtaagtaaa atattttgtt tggtctaatt gtttagtaaa ttggttatgg
     9721 ataagttttt tattaagtaa gtcggcataa ggttgaattt ttggttgaat ctccatacaa
     9781 aaaaaagtaa atgcttcttc taagctttta tcagtagtat cgcaagtcat ttttatttga
     9841 cgccaacaag catcttcact tactactgct tctaattcac tcaaatctaa aagccattgc
     9901 tctaaatctt ctttactatt aattattcta tctactaaat ttttaaaata aggttcaagc
     9961 ttatcccaag tagtaacagt aaattcaaga ggtaaaaaat ttcttgataa tttttttatg
    10021 tttatattta atggcatcgt aaaattttat tgctgcaaat gtagtgggtg atattaaaag
    10081 ttttggttgt tggtactagt tctttgttaa aactttttat agtgttagta ttaatataga
    10141 tatacaaata aataaatatt ttttagcaaa taaatcccaa aaaatatgct ttattatagg
    10201 tattaaaata ggtaacaccc tataaatttt aataaatagt taccttttat tgcatcatta
    10261 tcttatacta catttgcatc aatatattat ttatagttca attgtaaatg taaataagaa
    10321 ttataaaatt atatactaca actaatttac acagctaaaa atgtttgaaa taattttaga
    10381 aaatacagga aaaagattta ataaagaatg gatttttaaa gatcttcatt atgtatttaa
    10441 aagcaataat ttttatgcca ttgaaggggc aaatggtagt ggaaaaagca ctttgctgca
    10501 aataatagca ggaagtatgt tggcaagtaa tggtaacgta aaatataaaa ctaattcaac
    10561 agaaattgct tccgaaaaag tgtaccacca tgtagcaatt gctgctcctt atattgaagt
    10621 gattgaagaa atgacggcta acgagttttt acactttcat cacagtttta aacaattagt
    10681 attaccagta ccagaaattt tacaaataat agcattagaa aaagctgcca ataagcaaat
    10741 aagatatttt tcatcgggca tgaaacaaag aattaaattg gcccaggctt ttttttctaa
    10801 cactcctgtt ttgctgttag atgaaccttg tacaaatttt gatgcagcag ggtttaattt
    10861 atacgaaaac ctattaacaa attattgctc taataaatta gtaattattt gtagtaacga
    10921 tgaaaatgaa ataaaacaat gccactacag gttaaatatg ttgaatttta agacctaaat
    10981 aaaaatttta aaaattttgt aaccttttta taccttcctt actcatatat gtgtatatat
    11041 ttgtaaatga ttgaggacaa ttaactaaaa gatttttgct ttaaacaaca cacttttgtt
    11101 tgagtaaaca tctaacccat ttttcaaata ttatttttaa aaacttagta tttattatta
    11161 acaaagcaag atgcattata aaactttgtt gatagttata aaaatatcta ataaataaaa
    11221 tatggctaaa gtagctgttt acagaaaaga acattttaat gctgcacaca ggctacataa
    11281 taacaattgg agtgaggagc aaaataaatt aatttttggc aagtgtaata atgaaaatta
    11341 tcatggccac aattatgaat tagtggttaa agtaattgga gaacctaata ttgatacagg
    11401 atttgtgata gacactaaag agcttagtaa tttaattcaa aaagaagtta ttgaaaagtt
    11461 tgatcacaaa aatttgaata aagatatagc agcgtttaaa aacataattc caacagccga
    11521 aaatattgcc attgaaattt ataatatact tagaccgcaa attgaaacaa aattagaact
    11581 acaaattaga ttatatgaaa cagaaagaaa ctttgttgaa tatccgtctt aatgcagaaa
    11641 ataaaataga gcttagcgat atgatgatag acgaaatggg taacgaccat gtaagtacaa
    11701 gtgtggatac acctattaga gaagatgctt ttgaattatc ggatagtgaa aaagtaaaag
    11761 aaattgaaaa gcactttacc agtattatgc aaatattagg cttagattta actgatgata
    11821 gcttaaaagg aacccctaaa agagtagcca aaatgtatgt acaagaaatt tttaatggtt
    11881 taaatcctgc caataaacct tctattactt tatttgaaaa taaatatggg tataatgaaa
    11941 tgcttgtaga aaaaaatatt tcattttata cctgttgcga acatcatttt gtgcctttta
    12001 atggcaaggc acacatagct tatatcaaca gtggtaaagt aattggctta agtaaactaa
    12061 atagaattgt aaaatatttt gctaaaaggc cacaagtgca agaacgttta actatgcaaa
    12121 taggaaaaga attacaaaaa gttttgaata ctttagatgt tgcagtatta atagatgcaa
    12181 agcatttatg tgttgcaagc agaggtgtag aagatgatac aagtagcact gtaactgctt
    12241 tttatggtgg tgcatttaac gaggaagata aaaaaacgga atttttaaaa tatcttaact
    12301 tagaaactaa atactaattt ttaattatta catttattaa gccaaacttt gtttggcttt
    12361 ttttataatt tttcttaatc aatttttatg caaaaaaaga aacttgttgt atttactggt
    12421 gcaggcatta gtgctgaaag tggattgaaa acatttagaa gcaataatgg cttatggatg
    12481 aattataaaa ttgaagaagt agctacacca agaggctttg ctgcaaatcc gaaattagtt
    12541 ttagactttt ataatatgcg aagaagagaa gttgctgctg caaagcctaa caatgcacac
    12601 ttgcaactgg ttgacttaga aaaacttttt gatgtaacca ttattactca aaatattgac
    12661 gacttacacg aacgtgcagg cagtaagcat gtagtgcatt tacatggtca tatctttgaa
    12721 atgaaaagca cccaaaattc ttataaaatt tacaaaacaa atggagatat tcatgttgga
    12781 gatttggcag aagatggctt tcagttaagg cctaatattg tttggtttga ggagcctgtg
    12841 ccaatgataa atatagctga agaaatagct gccactgcag atatttttgt agttatagga
    12901 acatcattac aagtatatcc tgctgctggg ttaataaatt gcacaagggc atatattcct
    12961 aaatttatta tagataacga tattcctaaa gtagcaaaac acataaatca attaacagta
    13021 attgaaaaaa cagccagtga gggtgtagct gatttagtaa aaatattgaa tgaaattaag
    13081 taagctaatt aatcttcatt tacctctttc acttcttcat ctagcttatc tagtttatag
    13141 tatttaatag ttctggtaat ttcctttttc caaacattgg gtgttgtgct gtaaccagca
    13201 cctgcaatag catttatcca taatgtataa ttgttattgc ctttaagctt tttataatat
    13261 tttctgcggc ttaataattt acaaaaatct ctaaacgaat cttcatctgt ttcatattgt
    13321 ttaaatctgg ttttaatgcc ctttgtttta agtaaattat tccttcctat tataccaaaa
    13381 tgatttttta gcagcttaca atttctgctt cttccagcac cagattcaat aatacttaca
    13441 gcagtaatta tacttcttgg aatgccaaat tctttagata actcttcaac caggtcttta
    13501 tattttccaa tatacttatg tgtattattt gcctttacta ttgtacaaaa aagtaaacaa
    13561 ctaaaaacaa aaattatttt atacttcatc cacttattca tactattaat aagttgcttt
    13621 ttaagctctt ttaagtatta ttttaaacaa gataatcatt tcttataaaa ttctttaatt
    13681 tgggcatcta tttattcaat aataataata tgaaaaggtt ttttgttttt atttcatttc
    13741 tactatttct tgtagcttgt aaaaaaaata taacagataa tagtgcttca gtagcagctt
    13801 taacgttaac agatgttgct tatgctagtg caaattctaa tcaaaagtta gatgtgtatt
    13861 tgccagctaa tagaagtaca gcaactactt atacttgtat aatggttcat ggaggaagtt
    13921 ggagtggtgg agacaaatct gattttaata cagatattgc aaccctaaaa actttagtag
    13981 gtaactatgc tataataaat attaattata gtttggctaa tggtagctca atattattac
    14041 aacaacaaat agatgacctt aatgcagcat tcaattttat tcatagcaaa gccaacgaat
    14101 atttaattaa caccaataaa attgcaataa ttggtgctag tgctggtgca catttatctc
    14161 ttttaaaagc atataaatac aatactgata ataaaattaa agcaatggta gatttatttg
    14221 ggccaaccaa tatgacttgg atgtacacaa gtcatcctta tcctacaatt gcacaaccaa
    14281 caatcgttaa tgtaatgggt gcaacgccta ttaccaatgc agcagcttat tataatggta
    14341 gccctataaa ttttgtttcc tctacagtgc ctccaacaat aatttttcat ggcacagccg
    14401 atgaaattgt acccattagc gaaagtgtaa atttaaaaaa tgcactcgca accgcaggag
    14461 tacaatatga atattatact tacactggag aggctcatgg ttggacaggt gcaaacctta
    14521 cagatactta taataaagta attactttta ttaagaacta tgttaaataa tattttttaa
    14581 taaaaattat ttgccaccaa aaactttttt aagcaggttg gtggtttgtg caatagggtc
    14641 ttttcttatt tttaattctt cttgtgcaag ctgatagaaa ataccttcta aagccttctc
    14701 tgttacataa gaagccaaat ctggatttat ttttttgaag gaaaatttgt tataggttga
    14761 aaagactgta ttccaatgct gtgttgcatt tactttagct aaagatttgt caatgatagg
    14821 tctaaaagca tctgttaatg gtttgctggt ttgttgtttt aaatattttg ttgctgcaga
    14881 gtcgctacct tttaaaattt cccaagcatc tgcaaagctc atatttttga tggcattaat
    14941 aaaaataggt gcagcacttt ttgcagcatc ttcagctgca cggttcatag ataaaattgc
    15001 atcctctaca tacttacttc caccaggtat tttactaagt gcgtcttgca cttttttagc
    15061 ctcgggaggt aataaaattt taattgcttc gttcttaaaa aagccatcta cattagatag
    15121 ttgattagca cttttttctg caccaactgt taaagcttct tttaaacctt gtactacttc
    15181 ctctgtactt aaacctgatt ttgcgttatt atctttattg gttactttat ttattaaacc
    15241 tttaattact tgtgcttgta tattgcttgt aaaaattgta acaactaaaa tggctataaa
    15301 ttttttcata ataatttttt ggggtataaa tatccatact ttttaagtat tgaatcagct
    15361 ttaaaaatat tttaaattct ttataacata attaccaaaa taatgccata attacatata
    15421 ccaatatcta tatttatcta attcaattta taccacactt taaaccttta cttttgtagc
    15481 tttctacaat gattacgcca caaaccatag agcaaattaa aagcaggata gatattatag
    15541 atatcattag ccaatacgtt aatctaaaaa agcgtggtag taattatatt ggcaattgcc
    15601 cttttcataa cgaaaaaact ccatctttta ccgtatctcc tactaaagaa ttgtataaat
    15661 gttttggttg tggcaaaagt ggtaatgcaa taactttttt aatggaacat gacaaactaa
    15721 gttttgtaga agcattaaaa tggttggctg ctaaatacaa tattgaaatt gaagaaacag
    15781 aaacaacagc agagcaaaaa attgtaacac aaactgcaga tagcttatat atcttgaata
    15841 actttgctac caattttttg gagaaagaac tatggcaagg agaagaaggg gtagctattg
    15901 cattaactta tttaaaagaa agaggcttta gagaagatat cataaaaaaa tttcagttag
    15961 gatataatcc tacccaaaaa gataagttag ttactacatt actcaataac caatttaata
    16021 aagaattaat tccaaaatct ggtttaggtg ttgaaagaaa tcagcaggaa ttagtagata
    16081 attacagagg cagaattatt tttcctattc ataataatac tggtaaaata attgggtttg
    16141 gagcaagagt aattggaaat gcaaaagata aagcaccaaa gtatattaat acaccagaga
    16201 atgagattta tataaaaagt aaaattttat atggtatata ttttgctcgt tcacctatag
    16261 ataaagctaa tgaatgttta ttggtagaag gttatacgga tgtaataagc ttgcatcaag
    16321 caggaattga aaatgtagtt gccagtggtg gtacttcttt aactacagaa caaattagaa
    16381 taataaaaaa atttaccagc aacctcacca ttatttatga tggcgatagc tctggaataa
    16441 aagctgcttt gcgtggttta gatatggctt tagaagaagg ccttaatgta aaacttgttt
    16501 taataccaga taatgaagac cctgatagtt ttgtaaataa agttggtacc actgctttta
    16561 aagagtttat aaaaaataat aaaaaagatt ttataatttt tcagttagag gtaatactga
    16621 aagatgctgg taacgatatt gttttaaaaa ataatgcagt aaataatatt gccgataccc
    16681 taagcaaaat taacaggcca gaagatttta ccaagcttca agaatatatt aaacagtgtg
    16741 ctgctatttt aaaaatagat gaattaggct taactaatct agtaaataag tttaaagcag
    16801 ataaacttgc aaaagatgct aaaaagcaag caaataatac cacttttcaa caacaagaat
    16861 ttccagaaca aacaataaat actagcaatg atgatgttag cattttattt aaccaagatg
    16921 acgaaagcga aagaaattta ttaaaggttt tactagagca tggtttaaaa caatttaatc
    16981 aggaacaaac aattgcagaa tttattttta atgaattgaa tactttttat tttcaaaata
    17041 atttaattga aaccatagtt gaagaatata gaactgcata caacgaaggc aaacaacccg
    17101 aaccaaaaac atttttatac catactaata acgaaataag aaaccttgta attgccatta
    17161 cagctacacc ttatgaacta agtgttagat gggatgataa gttacaagga atgaatattg
    17221 ttaacagaga cacttctaca caagatgctt taataagtgt aaactttttc aagctcagaa
    17281 aaatcaagaa aatgatagat gaaaatcaac atgatttaga agtagaaaca gatttaagaa
    17341 agcaagaata ttatattaca gtacataaag agttaaaaga aaccgaaaaa aatattaccc
    17401 aaactttagg aactgttatt ctaaaataaa ttacaggtat tgttatattt tttgttcaat
    17461 ccaacctaac ttttgatatg cagcataaat ggcacctatt tgtacaccat gtataatttg
    17521 gctattgtgt atcatttcta aaacttcttt tttatccact aaaataacat ctaaatcttc
    17581 aaaagggtct ggttgttgac attgtaaatt ttcattggct ttagctaaaa aaaaataagc
    17641 catgttat
//
LOCUS       DAOQHP010000007        15540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1812_length_15540_cov_3.503261, whole genome shotgun sequence.
ACCESSION   DAOQHP010000007 DAOQHP010000000
VERSION     DAOQHP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15540
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_15540_cov_3.503261"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..3343)
                     /gene="secA"
                     /locus_tag="PLY81_00565"
     CDS             complement(17..3343)
                     /gene="secA"
                     /locus_tag="PLY81_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNF28751.1"
                     /translation="MIKFLSKLLGGNKSEKDVAAIQPYIIKINDFFKQYQLLTNDELR
                     NKTTEFKQRIKDYLTEIDTIIEDKKLTADNLPVQDVHSKDILYSEIDKLKKDRDKKIE
                     EVLEKLLPEAFAVVKETAFRFTNNTELKSTATNLDRDLSVQKDYVTIDGDQSIFKNTW
                     IAAGGQIIWNMVHYDVQLIGGSVLHQGKISEMATGEGKTLVSTLPAYLNALAGEGVHI
                     VTVNDYLAKRDSEWNGPIFEWLGLTVDCIDKHEPNTEDRRNAYLADITYGTNNEFGFD
                     YLRDNMVHNPDEMVQRKHHYAMVDEVDSVLIDDARTPLIISGPVGKDTSTEQFFELKP
                     RIERIVEAQKKSVNQFLIEAKKKIAEGNDDPKDGGLALFRAHRGLPKNTALIKFLSEP
                     GIRVKLQKTENYYLADQQKEMPKADAELYFYIDEKNNSVELTDKGIVLITRSGEDPNF
                     FILPDISVALAEIENNSNYTTDEKLHKKEVVITEYSIKADRIHTVQQLLKAYTLFEKD
                     DEYVVIEDQVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKIEATTQTYATVTLQNYF
                     RMYHKLAGMTGTAETEAAELWDIYKLDVVSIPTNKTLIREDEQDKVYKTKREKFGAVI
                     DEIVKQKEAGRPSLVGTTSVEISELLSRMLRQKQIPHNVLNAKQHAREAQIVAEAGLA
                     GAVTIATNMAGRGTDIKLGPNVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSS
                     QFFVALEDDLMRMFGSERIAKVMDFAGYKEGEVIQHSMITKSIERAQRKVEENNFGIR
                     KRLLEYDDVMNKQRTVIYTKRHHALFGKRLALDIDNAFYSVAKGLINNFKAINDFEGF
                     KLAVIVNFGIDTSINIEELNKANDNSLTEKLYSEAINNYQRKKQEIALKSVPVFKNIR
                     LHQGNNIENVVVPFSDGKKTLQVLASMQKTLDSEGVELLDSLERNLALALIDEAWKEH
                     LRAMDDLKQSVQTATYEQKDPLVIYKMEAYNLFENMNAEVNKNIVEFLCHCGIPSDEE
                     GQQLKEGRQQKTDLSKTSNNKDDIDSRGDSYGANENDYYDPSANVKQQPVIAAPKIGR
                     NDLCPCGSGKKYKACHGKDA"
     gene            complement(3615..5126)
                     /gene="gltX"
                     /locus_tag="PLY81_00570"
     CDS             complement(3615..5126)
                     /gene="gltX"
                     /locus_tag="PLY81_00570"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNF28752.1"
                     /translation="MNKKVRVRFAPSPTGGLHLGGVRTVLFNYLFAKQHNGEFVLRIE
                     DTDQTRFVEGAEQYIYDTLDWCGLVPDESPLHGGNFAPYKQSQRKDLYKQYALKLVED
                     GFAYYAFDTANELELKRNSIPNFQYNHITREGMKNSLVLDETTTKELLKSGLPYVIRM
                     KMPHNEDIRFNDMIRGEIHFNSSLVDDKVLLKADGMPTYHLAVVVDDYLMQISHAFRG
                     EEWLPSAPVHILLWKYLFGLNNMPQWAHLPLILKPDGNGKLSKRDGDRLGFPVFAKDW
                     YDPKTNETTVGFKERGFLPQAFLNMLVMLGWNDGTEQEIFTLQEMVEKFSIERVHKGG
                     AKFDYEKAKWFNHAWIMKLDINSLNLQVKNVLGSLTVGYSDSYINQVINLTKDRCTFI
                     ADFEQQTSYFFKAPINVDVAAIKPKWDNNKQHFFEEYLNWLNTNFDWSTSILEQEFKN
                     LASANGLKHGELMLPFRIMLVGGKFGPAVFDIANIIGKEQTITRIKHVLNLLA"
     gene            5189..5968
                     /locus_tag="PLY81_00575"
     CDS             5189..5968
                     /locus_tag="PLY81_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF28753.1"
                     /translation="MNVRTMADMMANNETPEILFWIGCAGSFDQRAQKITKAFAAILD
                     KTGIKYAILGKEEACTGDPARRAGNEFLFQMMAYQNIQILNGYGIKKIVTTCPHCLNI
                     MKNEYPELGGNYEVIHHTTLLQQLINDGKIKLKEGGSFKGKKITYHDSCYLGRANNIY
                     EAPRKVLEALDAELVEMKRCGSKGLCCGAGGAQMFKEEEAGNKRINIERTEEALETKA
                     NFIASACPYCNTMMTDGVKLKEKEADVQVLDIAEMIANEME"
     gene            5988..6194
                     /locus_tag="PLY81_00580"
     CDS             5988..6194
                     /locus_tag="PLY81_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28754.1"
                     /translation="MKNFNVKKFIKATLILLPILLLVDIVYDMIFKELILKETFAMKN
                     LFFKIAASLVGAYFFATSKTKEEN"
     gene            6232..6741
                     /locus_tag="PLY81_00585"
     CDS             6232..6741
                     /locus_tag="PLY81_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28755.1"
                     /translation="MDISYSHLIPTNFSDNAKVWIYQSSRKFTINEALQIEAILDDFI
                     AQWQSHGIPVKGFATLFFGQFLVFMADDNVDVSGCSTSSSVDVVKQVEKIFNVEMFNR
                     QNLAFVVKDEVQLLPLAQLNYALSNSFITANTLYFNNTVTNKNTLLSNWIIPVNKSWL
                     KTKLSNLSA"
     gene            7062..10190
                     /locus_tag="PLY81_00590"
     CDS             7062..10190
                     /locus_tag="PLY81_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF28756.1"
                     /translation="MRKLVMLMACLTLFISQILAQNSRTISGKVTDENGAAISNASII
                     VKGTTIGTTTANDGTFTIKVPENAKSLIISSVNFMTVEVSISKESTVNVSLQSTSANL
                     EEVVVVGYGTQKKKEVTGAVSQVNPVNISTLVTPSVDKQLGGRATGVLVTNSSGLVNQ
                     PPRIRIRGVNSINGSSSPLFVLDGVPMESGGFAGYTSDNLLANINPADIESIDVLKDG
                     SATAIFGSRAANGVIMITTKKGKAGKLNVNYSFIQGSSKPSKKFKLLNAQEFVLITNE
                     KLTNAGQQPAAFMNNENTDTDWQSYVYRNNAKSINHNLSIDGGTDKTNFFVSFNYSDQ
                     QGLVFTNTVKRYNIRANVTQKINKWLKLSNLITLSRTEDSDQNNGGNSLSGATANVLR
                     ALPNVRVMNPSLPQFSFYNVTPDGAALGSDANTKLIENNYTNIAYVMAKNKFFSKKHR
                     VINNFGIDLKPLSWLNYSFKANIDFITLDEFLTYDPKHGDGRNSIGRVQNQAANQMRY
                     TIQNFININKSFKEHNVAVTLGHENQMQTSNSFQAIGTNISDPFFQQTNVISNSYATQ
                     QSSGSYSKGPGFVSYFGRVNYDYAGKYFVTASLRRDGLSRFAPENRFGTFPGFSVGYR
                     VSQEDYWKKSPMAKVINEFRVRTSWAKVGNAEIAGGSFPYLSLYGSAPYGAISGIAAT
                     QAGNAGLKWETNEKLGFGFDLGLLDNKINITFDYYKNNNNNLVLAAPQPPSFGVPGNL
                     IYQNIGNMENKGIELAINSKIINKKDFSWDFGINFTTQSNKVKSLYLDQDVIVSNGTG
                     NYNILQVGQPINAIYGYQFAGVNSSNGNPVYVKADGTTLVMGNITNSSYFTISDLNSN
                     VVGAASSLTGADRKVLGSSLPKWFGGITSTIRYKQFTLDMLWRFSGGNKIMNITEQES
                     LLNQAFMNSGKNILKRWTQAGDVTTVPRLWYNRDNFTNLNQQAVDRFVESGNFIRLDN
                     LQISYDVESKHITKLTNSYVKSFRVFVQGQNLLLITKYTGIDPDNIDSRGLDYNTVPQ
                     ARSISFGVNVGF"
     gene            10217..11818
                     /locus_tag="PLY81_00595"
     CDS             10217..11818
                     /locus_tag="PLY81_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF28757.1"
                     /translation="MKFKIFSAVIIAGSISLFSCQKKTLELQPFSSFSDQSAFSNADR
                     CQLALNGVYDAAQSGFYAGGAVRGYPFGAANIEQGEMRGEDMLNQATFFAITYAATYN
                     SASPNNGYMFQTIYSMVNKANLVIDGVKAAGTGGVITAAQALQFEAECRFLRALGHHE
                     LVINFSRPFADGAGNKMGIIYRDFAINSDASSAAARAIKRSTITVADNYTKILEDLDF
                     AETNLPATQTGAGLSTYRATKAAAIALKMRVKMHKGDWAGVIAEGAKIVTGTAPNFLS
                     PIGGWKLTAAPDGPFANNTSTESVFSIKNDATDNPGVNGALAQMFGDPALSARGLVRV
                     SPIMWSEADWKCNDLRRTALYRTAGGPVNAVYSTKFRDYSTTSDGTPQIRYAEVLLML
                     AEANARTSTIVSSTAVDLLNAVRNRAVTTPGDQFTLLSFATKNDLIAAILKERRIELL
                     GEGKRWGDIHRLALDPDFAPIAGGGIPAKVGTGGATAAMFNCAGGVTITKSILAIPYS
                     DFKFVWPIPIEETQQNANYDQNPNY"
     gene            11915..12934
                     /locus_tag="PLY81_00600"
     CDS             11915..12934
                     /locus_tag="PLY81_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNF28758.1"
                     /translation="MNLHYYTYNLPFEYPFEISGKRIKTHQPTLIVILELGNFYGVGE
                     ATAINYYNVTTEKMVDVIEKNKKMIEKFAFIQPERYWHYLHHLIPDSPFLTCALDMAA
                     WDLYGKMQKNNLYKLWNTDLENYPATSYTIGLDSLDKMVEKVKAKPWNSYKIKLGVEN
                     DIEIIEAIRKHTNAKIRIDVNGAWQTKEAIDKINILKDFNIELIEQPLTKDNWEGMKT
                     LFNQTTIPLFADESCVHEEDVVKCNGYFHGINIKLTKCSGITPAIRMIEEAKSLGLKV
                     MMGSMNESSIGTSAIANFLPQLDYVDMDGPLLLSKDLAIGLQISPSKVTLDGKLGLGI
                     QPTLF"
     gene            13068..13634
                     /locus_tag="PLY81_00605"
     CDS             13068..13634
                     /locus_tag="PLY81_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF28759.1"
                     /translation="MQVQFINSENTQIQKCIEGCRKSQKELYDFYAAKMFAVCLRYAK
                     NDMDAEDILQDGFVKIFTNLDKYRADGSFEGWLRRIMVNTAIEHIRKNKINNQDISEN
                     IENTVKDKYANALDKLFEKELVNIASTLSDGYKAVFNMYAIEGYSHKEIAKQLGITES
                     TSKSQFSRAKSILRNLLQVNNNQNVIAC"
     gene            complement(13782..14627)
                     /locus_tag="PLY81_00610"
     CDS             complement(13782..14627)
                     /locus_tag="PLY81_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF28760.1"
                     /translation="MQLLINFTQILLTTQITPVLFLGTIGMILLATSIILFAIFHQRR
                     VMRFQQRIQQMEVEHQKMLLKASIKLQEEERTRLAADLHDDAGPLLATAKLYLNDNFV
                     NLDKTAQMHSLFNAKQIIDDTIQLIRNISHSLMPPTLKNFGLSSAVNDFFQKISNSGS
                     LNASSKFHDYKTRLKSDKELILFRVIQELVNNVLKHSKASFIHLTQHDVEEKVILRLH
                     HDGFGLTQEEFLRYTNEKEGLGLKNIQSRLRVIHGDILYEKDSSQSLYKVTIEIPREE
                     GEEIK"
     gene            complement(14634..15329)
                     /locus_tag="PLY81_00615"
     CDS             complement(14634..15329)
                     /locus_tag="PLY81_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28761.1"
                     /translation="MNYLFHISVFSPLIPILFFVLFKFNSKQRAKWVIFLLLVISLIT
                     DIWCYNLARNNIKTTWIINIFSVIEFAFLSYFFTLIISLKKQQIFIWLCYAGFLLYWL
                     VQNIFVESIEIFDYQSQAIEFLLLLICCLLYLFQRVKNVRGEILYNTYQFWIVAALLM
                     YCAGTFFGFLSSVSPEERDFDTIVYEYLSRGGNILKHILITIAFCINPANQKNEHPNK
                     NSIYYIKDNLGKD"
     gene            complement(15389..15511)
                     /locus_tag="PLY81_00620"
     CDS             complement(15389..15511)
                     /locus_tag="PLY81_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28762.1"
                     /translation="MESLQKIKKDVIKHKGIVGDRSAICPPSCGQGDMDLDWSS"
BASE COUNT         5285 a         2472 c         2354 g         5429 t
ORIGIN      
        1 tttttaggct gatggtttat gcatctttac catgacaggc tttgtacttt ttaccactac
       61 cacaagggca aaggtcattt ctgcctattt tgggtgcagc aattacaggt tgttgtttaa
      121 cattggcaga tggatcgtaa taatcgtttt catttgctcc atagctatct cccctgctat
      181 caatatcatc tttgttattg cttgttttgc ttaaatctgt tttttgctgt ctgccttctt
      241 ttagttgctg cccttcttca tctgatggaa taccacaatg acacaaaaac tccacaatat
      301 ttttatttac ttcggcattc atgttttcaa ataaattgta tgcctccatt ttgtaaataa
      361 ccaatgggtc tttttgttcg taagttgccg tttgtacact ttgctttaaa tcatccatag
      421 ctcttaaatg ttctttccac gcttcatcta taagtgctaa tgcaaggttt ctttctaaag
      481 agtctaaaag ttctacacct tctgagtcta atgttttttg catagatgca agtacctgta
      541 aggttttttt accatcgcta aaaggtacta ccacattttc aatattgtta ccttggtgta
      601 aacgaatatt tttaaataca ggaaccgatt ttaaggctat ttcttgtttt tttctttggt
      661 aattatttat tgcttctgaa tataactttt ctgttaatga attatcgttg gctttgttaa
      721 gttcttcaat attgatgctt gtatctatac caaaatttac aataaccgct aatttaaaac
      781 cttcaaaatc attaatagct ttaaaattat taattaaacc ttttgccaca ctgtaaaaag
      841 cattatcaat atctaaggct aatcttttac caaataatgc atgatgacgt ttggtataaa
      901 taacagttct ttgtttattc attacatcat cgtactctaa tagccttttt ctgataccaa
      961 agttgttttc ttcaactttt ctttgagctc gttcaatact ttttgtaatc atactatgct
     1021 gtataacctc tccttcctta tatcctgcaa aatccatcac ttttgcaatt ctttcgctgc
     1081 caaacattct cattaaatca tcttctaacg caacaaaaaa ttgagaagaa cctggatctc
     1141 cttgacggcc tgcacgtcca cgaagctgtc tatctaccct tctggattca tgtctttctg
     1201 tgcctataat tgccaaccca ccagcttctt taacattagg cccaagttta atatctgtac
     1261 cacgaccagc catattggta gcaattgtta ctgcacctgc taaacctgct tcagcaacaa
     1321 tttgagcttc tctggcatgt tgcttagcgt ttaatacatt atgtggaatt tgtttttggc
     1381 gtaacattct gctaagtaat tcacttattt ctacagatgt tgtaccaacc agtgatggcc
     1441 tacctgcttc tttttgtttt acaatttcat ctattacagc accaaacttt tctctttttg
     1501 ttttatacac tttatcttgc tcatcttctc ttattaaagt tttattagtt ggaattgaaa
     1561 ctacatctaa tttataaata tcccaaagtt cagcagcttc agtctctgca gtacctgtca
     1621 ttcctgctaa tttatggtac attctaaaat agttctgtaa ggttactgtg gcgtatgttt
     1681 gagtagttgc ttcaattttc acgttttctt tagcttcaat agcctggtgc aatccatcac
     1741 tatatcttct tccttccata atacgaccag tttgttcatc aactattttt acttggtctt
     1801 caattactac atattcatca tctttttcaa ataaagtata tgcttttagt aattgttgaa
     1861 cagtatgtat tctatctgcc tttatagaat attcggtaat tacaacttct tttttatgta
     1921 atttttcatc ggttgtataa tttgaattat tctctatttc tgccaatgca acacttatat
     1981 caggaagtat gaagaaatta ggatcttcac cggaacgagt tattagcaca attcctttat
     2041 cggtaagttc aacactattg tttttctcat caatgtaaaa atataattca gcatcggctt
     2101 taggcatttc tttttgctga tcggctaaat aataattttc tgttttttga agctttactc
     2161 taatacctgg ttcacttaaa aacttgatta aagctgtatt ttttggtaag cctctatgag
     2221 ccctgaataa tgctaaacca ccatctttgg ggtcatcatt accttctgca attttctttt
     2281 tagcttcaat taaaaattgg tttacacttt ttttctgggc ttctactatt ctttcaattc
     2341 ttggtttaag ttcaaagaat tgctccgtac tagtatcttt tcctactggg ccgctaataa
     2401 ttaaaggagt tctagcatca tctattaaaa cactatccac ttcatctacc atggcataat
     2461 gatgtttacg ttgaaccatt tcatctgggt tatgtaccat gttatctcta agataatcaa
     2521 acccaaactc attattagta ccataagtta tatctgctaa ataggcattt cttctatctt
     2581 ctgtattagg ctcatgttta tctatacaat caactgtcaa gccaagccat tcaaatattg
     2641 gtccattcca ttcactatca cgttttgcta aataatcgtt tacagttaca atatgcaccc
     2701 cttctcctgc tagagcatta agataagcag gtaatgttga taccaatgtt ttaccttctc
     2761 ctgtagccat ttcagatatt ttaccttgat gaagtactga gccaccaatt agctgcacat
     2821 cataatgtac catattccat attatctgac cacctgctgc tatccaagta tttttaaaaa
     2881 tggattggtc tccatcaatg gttacataat ctttttgaac acttaagtct ctatctaaat
     2941 tagttgcagt agattttagt tctgtattat tagtaaacct aaatgcagtt tcttttacta
     3001 cagcaaaagc ttcgggtaat aatttttcta aaacttcttc tatcttttta tctctgtctt
     3061 tcttaagttt atcaatctcg ctatataaaa tatcttttga atgaacatct tgaacaggta
     3121 aattatcagc agttaatttt ttatcttcaa tgatggtatc aatttcagtt aaataatctt
     3181 ttattctttg cttaaattca gtagttttat tacgcaattc atcatttgta agtaactggt
     3241 attgcttaaa gaagtcatta atttttataa tatagggttg aatggctgca acatcttttt
     3301 cacttttatt acctcctaaa agttttgata gaaacttaat catattggtt attatttatg
     3361 cacttttctt ctgtaataat ctcttttatc ctacttcaat taatactaat aattgtgcaa
     3421 ttaaatttta gttattcttt tatatttctt gaaaaaactt agtagaaaat tttatgccta
     3481 atttatttta cataaaaatg gttacatcta aaacaaatta ttagccaaat tggcactaat
     3541 gctttttaag gctgccaaag ttaacagatt ttagaatatt gaaatgtact aaaatgctaa
     3601 aatttttaac tagtttaagc cagtaaattt aatacatgct taattcttgt tatagtttgt
     3661 tctttgccaa taatattggc aatatcaaaa actgcagggc caaattttcc acctacaagc
     3721 attattctaa aaggcaacat cagttcgcca tgttttaaac cattggctga tgctaaattt
     3781 ttaaactctt gttctaatat ggatgtactc caatcaaagt tggtatttaa ccaattcaaa
     3841 tattcctcaa aaaaatgttg tttattatta tcccattttg gtttaatagc agctacatca
     3901 acatttatag gggctttaaa aaaataagaa gtttgttgtt caaaatctgc aataaaagtg
     3961 catctatctt tagttaaatt aataacttga ttaatataag aatctgaata tcctactgtt
     4021 aaacttccta aaacattttt aacttgaagg tttaaactat taatatctag tttcattatc
     4081 catgcatggt taaaccattt ggctttttca taatcaaatt ttgcccctcc tttatgaaca
     4141 cgttcaatag aaaatttttc taccatttct tgtaacgtaa atatttcttg ttctgtgcca
     4201 tcattccatc ctaacataac caacatattt aagaaggctt gtggtaaaaa accacgttct
     4261 ttaaagccaa ctgttgtttc atttgtttta gggtcgtacc agtccttggc aaatacagga
     4321 aatccaagtc tatcgccatc tctcttactt aatttaccat taccatctgg ttttaaaatt
     4381 aaaggtaaat gagcccattg aggcatattg tttaatccga ataaatactt ccaaagtaaa
     4441 atatgtactg gagcacttgg tagccattct tctcctctaa atgcatggct tatttgcatt
     4501 aaataatcat ctactacaac agctaaatga tacgttggca ttccatctgc ttttaataaa
     4561 actttatcat ctaccaacga agagttaaaa tgaatttcgc ctcttatcat atcattaaaa
     4621 cggatgtctt cattgtgtgg catcttcatt cttataacat aaggtagccc agatttcagt
     4681 aattctttgg ttgtagtttc atctaatacc aaagagtttt tcataccctc tctagtaata
     4741 tggttgtatt ggaaattagg aatgctgtta cgttttaact caagttcgtt agcagtatca
     4801 aatgcataat aagcaaaacc atcctccaca agtttaagtg catattgctt atataaatct
     4861 tttctttggc tttgtttata tggtgcaaaa ttacctccat gcaaaggact ttcatcaggc
     4921 actaatccac accaatctaa ggtatcataa atatattgtt cagcaccttc tacaaatctt
     4981 gtttggtctg tatcttcaat tcttaataca aattcgccat tatgttgctt agcaaataaa
     5041 taattaaaca aaactgttct tacaccacct aaatgtaagc cacctgttgg gcttggagca
     5101 aaacgaacac gtactttttt attcataagt ttgcaaagat aagcccttcg tttttttcct
     5161 tattttcgca acactaattt gtttgtttat gaatgttaga acaatggcag atatgatggc
     5221 aaataatgaa acgcctgaaa ttttattttg gattggttgt gctggtagct ttgaccaacg
     5281 tgcacaaaaa ataactaaag cctttgctgc tatattagat aaaacaggta ttaaatacgc
     5341 aattttaggt aaagaagaag catgtactgg cgaccctgca agaagagctg gtaatgaatt
     5401 tctatttcaa atgatggcct atcaaaatat ccaaattcta aatggatatg gaataaaaaa
     5461 aattgtaaca acctgcccac attgtttaaa tataatgaaa aatgaatacc ccgaattagg
     5521 gggtaattac gaagtaatac atcatacaac tttactacaa caactaatta atgatggtaa
     5581 aattaaactt aaagaaggtg gcagctttaa aggcaaaaaa attacttatc acgatagttg
     5641 ttatttaggt agagccaata atatttatga agcacccaga aaagttttag aagctttaga
     5701 tgctgaatta gttgaaatga aacgttgtgg cagcaaaggt ttatgctgtg gtgcaggtgg
     5761 tgcacaaatg tttaaagaag aagaagcagg aaataaaaga ataaatattg aacgtacaga
     5821 ggaagcatta gaaacaaaag caaactttat tgcatctgcc tgtccttatt gcaatacaat
     5881 gatgaccgat ggtgtaaaac taaaagaaaa agaagcagat gtgcaagtat tagatatagc
     5941 agaaatgatt gccaacgaaa tggaataatt ttataaaaaa ttaatgaatg aaaaacttta
     6001 acgtaaaaaa atttataaaa gcaaccttaa tattactacc tattttgctt ttggttgata
     6061 tagtgtacga catgattttt aaagaattaa ttcttaaaga aacctttgca atgaaaaatt
     6121 tatttttcaa aattgcagct tctttagttg gtgcttattt ttttgccact tctaaaacga
     6181 aagaggaaaa ctaactttaa tttttatttc ataaaataat taattatttg aatggatatt
     6241 tcttattcac acctaattcc aaccaatttt agcgataatg caaaagtttg gatttaccaa
     6301 agtagtagaa aatttacaat aaacgaagct ttgcaaattg aagctatttt agacgacttt
     6361 attgcacaat ggcaaagcca tggcatacct gtaaaaggtt ttgctaccct gttttttgga
     6421 cagtttttgg tttttatggc agatgataat gttgatgtta gtggctgtag cactagctcc
     6481 tcggtggatg ttgtaaaaca agttgaaaaa atatttaatg tagaaatgtt taaccgacaa
     6541 aatttggcat ttgttgttaa agatgaagtt cagttattgc ctcttgcaca gttaaactat
     6601 gcattatcca atagttttat tacagcaaat acattatatt ttaataatac agttaccaat
     6661 aaaaatactt tgctaagtaa ttggattatt ccagtaaaca agagttggtt aaaaactaag
     6721 ttaagtaacc tttctgcata acatagaatt aaaataaaat ttacactttt catttgttaa
     6781 aaaagtaaga tgggtatata ctttaagaaa ctattttctt gaattttttt ttatttattt
     6841 ttagaatagt attacacatt gaatatcaat aaaattcaat aatttattca tttttaatat
     6901 tagttatttt tcaaaaattc cttctcaatt gtagtcttaa atttatagaa ttcaactact
     6961 tattaacata taaaaaaata ttattttttg tatgcagcat tcatttattt ttatagtcta
     7021 ttaacttttt gttaaccatt cttaacctta aaccaaagca aatgagaaaa ttagtcatgt
     7081 taatggcttg tctgacatta tttattagtc agattcttgc ccaaaattct cgtaccattt
     7141 ccggaaaagt aactgatgaa aatggagcag ctatttctaa tgcatccatt atagttaaag
     7201 gaacaaccat tggtacaact actgcaaacg atggtacttt tacaattaaa gtacctgaaa
     7261 atgcaaaatc attaattatt tcttcggtaa attttatgac cgtagaagta tctatctcaa
     7321 aggaatctac tgttaacgtt agcctacaat caacttctgc caatttagaa gaagttgtag
     7381 tagttggtta cggtactcaa aagaaaaaag aagttacagg tgctgtaagt caagtaaatc
     7441 ctgttaacat ttctacttta gttactccta gtgtggataa acaactaggt ggtagagcta
     7501 ctggtgtttt agttactaat tcaagtggtt tagtaaatca gccaccaaga attagaattc
     7561 gtggtgttaa ctctattaat ggcagttcat cccctctatt tgtattagat ggtgtaccga
     7621 tggaatctgg tggttttgca ggttacacaa gcgataattt attagcaaat attaaccctg
     7681 cagatattga atctatagat gtgcttaaag atggtagtgc tacagccatt tttggttcta
     7741 gggctgcaaa tggtgtaata atgataacta ccaaaaaagg taaagcaggt aaattgaatg
     7801 ttaattacag ttttattcaa ggatcttcta aaccttctaa aaagtttaaa ttattaaatg
     7861 cacaagaatt tgttttaatt acaaacgaaa agctaactaa tgctggtcag caacctgcag
     7921 catttatgaa caatgaaaac acagataccg attggcaatc ttatgtgtat agaaataatg
     7981 caaaatctat taaccataat ttatctattg atggcggcac tgataaaaca aacttttttg
     8041 tatcattcaa ttattcagac caacaaggtt tagtttttac aaatactgtt aaaagatata
     8101 atatacgtgc taacgtaact caaaagataa acaaatggct taaattaagt aaccttatta
     8161 ctttatcaag aacagaagat tcagaccaaa acaatggtgg aaactcatta tctggagcaa
     8221 ctgcaaacgt tttaagagca ttacccaatg ttagagttat gaatccttct ctacctcaat
     8281 ttagttttta taatgttaca ccagatggag ctgctttagg ctctgatgca aatactaaat
     8341 taattgaaaa caactatacc aatattgctt atgtaatggc taaaaacaaa ttttttagta
     8401 aaaaacacag agtaataaac aattttggta tagatcttaa accattaagc tggttgaatt
     8461 atagctttaa agcaaatatt gactttatta ctttagatga atttttaact tacgacccta
     8521 aacatggtga tggtagaaac agtattggac gtgttcaaaa ccaagctgct aaccaaatga
     8581 gatataccat tcaaaacttc attaatataa ataaatcatt taaagaacat aatgttgctg
     8641 ttacattagg ccacgaaaac caaatgcaaa cttcaaatag ctttcaagct attggtacaa
     8701 acatttcaga tccattcttt caacaaacta acgtaattag taatagctat gcaacacaac
     8761 aatctagtgg tagctatagt aaaggtcctg gatttgtatc ttactttggg cgtgtaaact
     8821 atgactatgc tggtaaatat tttgtaactg catctctaag aagagatggc ttatctcgtt
     8881 ttgcaccaga gaatagattc gggactttcc caggtttttc agtaggttat agagtttctc
     8941 aagaagatta ttggaaaaaa agtccaatgg caaaagttat taatgaattt agagtaagaa
     9001 cttcttgggc taaagtaggt aatgcagaaa ttgcaggtgg tagtttccct tatttaagct
     9061 tatatggtag tgctccttat ggtgcaatta gtggtattgc tgctacacaa gctggtaatg
     9121 caggattaaa atgggaaaca aatgaaaagc taggttttgg ctttgactta ggcttactag
     9181 ataataaaat taatatcact tttgattatt ataaaaataa taacaacaac ttagttttag
     9241 cagctcctca gccaccttct tttggagttc caggtaactt aatttatcaa aacattggta
     9301 atatggaaaa caaaggaatt gagttggcta ttaattctaa aataatcaat aaaaaagact
     9361 ttagttggga ttttggtata aactttacta cacaaagtaa caaagtaaaa tctttatact
     9421 tagaccaaga cgttatagta tctaatggta ctggtaacta caatatatta caagttggcc
     9481 aaccaattaa tgctatttat ggttatcagt ttgcaggtgt aaattcatct aatggtaacc
     9541 ctgtttatgt taaagcagat ggaactactt tagttatggg taatattact aactcttctt
     9601 actttactat ttcagattta aacagtaatg ttgttggtgc tgcatcttct ttaacaggag
     9661 ccgacagaaa agttttaggt agcagtttac caaaatggtt tggaggtata acaagcacta
     9721 ttcgttataa gcagtttact ttagatatgt tatggagatt ttcaggcgga aacaaaatca
     9781 tgaatattac cgagcaagaa tctttattga accaggcatt tatgaatagt ggtaaaaata
     9841 ttttaaaacg ttggacacaa gcaggagatg taactacagt accacgttta tggtataaca
     9901 gagataattt taccaactta aaccaacaag ctgtggatag atttgtagaa agtggcaatt
     9961 ttataagatt agataattta caaataagct atgatgtaga aagcaaacat attacaaagc
    10021 ttaccaactc ttatgtaaaa tcatttagag tatttgtaca aggtcagaat ttattattaa
    10081 ttacaaaata cacaggaata gatccagata atattgattc tagaggctta gactacaata
    10141 cagtaccaca agctagatca atttcttttg gagtgaatgt tggcttttaa ttatataacg
    10201 cttaaaaaca ttaaatatga aatttaaaat attttcagca gtcattatag caggtagtat
    10261 aagcctgttt tcctgccaga agaaaacttt agaactacaa cctttttctt cattttcaga
    10321 ccaatctgca ttttctaatg ccgatagatg ccaattagca ttaaatggtg tttacgatgc
    10381 tgcacaatct ggcttttatg caggtggtgc agtaagaggc tatccttttg gtgcagctaa
    10441 tattgaacaa ggagaaatga gaggagaaga tatgcttaac caagcaacat tttttgcaat
    10501 aacttatgct gcaacctata atagtgcatc tcctaacaat gggtatatgt ttcaaactat
    10561 ttatagtatg gtaaacaaag ccaatttagt gatagatggt gtaaaagcag caggcacagg
    10621 tggcgttatt actgcagcac aagcattaca attcgaagct gaatgtagat ttttaagagc
    10681 tttagggcat catgaacttg taataaattt ctcaagacct tttgccgatg gtgcaggtaa
    10741 taaaatggga attatttaca gagattttgc aatcaattcc gatgcatcat ctgctgctgc
    10801 aagagcaatt aaacgttcta ccattactgt tgcagataat tatactaaaa ttttagaaga
    10861 tttagatttt gctgaaacta atttaccagc cacacaaaca ggagccggct taagtacata
    10921 ccgtgcaact aaagcagctg caattgctct taaaatgagg gttaaaatgc acaaaggtga
    10981 ttgggcaggt gttattgctg aaggtgcaaa aattgttacc ggtaccgcac ctaatttctt
    11041 aagcccaatt ggtggatgga aattaactgc agcacctgat ggaccttttg ctaataacac
    11101 ttctacggaa agtgtattct ctattaaaaa tgatgcaaca gacaatccag gtgtaaatgg
    11161 tgcacttgct caaatgtttg gagaccctgc actaagtgct agaggtttag ttagagtaag
    11221 cccaattatg tggagcgaag cagattggaa atgtaatgat ttacgtcgta ctgctcttta
    11281 cagaactgct ggcggccctg ttaatgctgt gtattcaaca aaatttagag actactccac
    11341 tacatctgat ggtacaccac aaattagata tgcagaagtt ttattaatgt tagcagaggc
    11401 taacgcaaga acttcaacaa ttgtttcttc aactgctgta gatttattaa atgcagtaag
    11461 aaacagagca gttacaactc ctggagatca gtttacatta cttagttttg caactaaaaa
    11521 cgatttaatt gctgcaattt taaaagaaag aagaattgag cttttaggag aaggtaaacg
    11581 ctggggggat attcatagat tagcattaga cccagatttt gctccaattg ctggtggtgg
    11641 tattcctgct aaagttggta ctggtggtgc aactgctgca atgtttaatt gtgcaggtgg
    11701 tgtaactata actaaaagca ttttagcgat accttacagc gactttaaat ttgtttggcc
    11761 aataccaatt gaagaaactc aacaaaacgc taattacgac caaaacccta attattaatt
    11821 ttttcaaggg gttataattt taagctgcac ctattaggtg cagctttttg ttttatagag
    11881 ttgataatat tttaaataat acatttgcat atttatgaat ttacattatt atacttataa
    11941 cctgcctttt gaatatcctt ttgaaatttc tggaaagaga attaaaactc accaaccaac
    12001 cttaattgta attttagaat taggaaactt ttatggagtt ggagaagcaa cagcaataaa
    12061 ttattataat gtaacaactg aaaagatggt tgatgtaata gaaaagaata agaaaatgat
    12121 agaaaaattt gcttttattc aaccagaaag atactggcat tatttacacc atttaattcc
    12181 tgatagccct tttttaactt gtgctttaga catggctgct tgggacttat atggtaaaat
    12241 gcagaaaaat aatttataca aactatggaa tacagattta gaaaattacc cagctacctc
    12301 ctatacaatt ggcttagata gtttagataa aatggttgaa aaagtaaaag caaaaccgtg
    12361 gaatagttat aaaattaaat taggagtaga aaatgatata gaaattattg aagcaataag
    12421 aaaacatact aatgcaaaaa ttagaattga tgtaaatggt gcctggcaaa ccaaagaagc
    12481 aatagataaa attaatattt taaaagactt taatattgaa ttaattgagc aaccattaac
    12541 caaagataat tgggagggaa tgaaaacatt atttaaccaa accaccattc ccctatttgc
    12601 agatgaaagt tgtgtgcatg aagaagatgt agttaaatgt aatggatatt ttcatggcat
    12661 taatattaag cttaccaaat gcagtggaat tactcctgct attagaatga ttgaagaagc
    12721 aaaatcatta ggtttaaaag taatgatggg aagtatgaat gaaagcagca ttggcacttc
    12781 ggcaattgct aattttttac ctcaattaga ttatgtagat atggatgggc ctttattact
    12841 ttctaaagat ttagctattg gcttacagat ttctccttca aaagttactt tagatggaaa
    12901 acttggatta ggtattcaac ctaccctttt ttagtcttct cattttggga aaggtagtcc
    12961 ttcaagggga ttttaaacta gactatcttt aatttaacta cttaataatc aatcatttac
    13021 aattttggca ttttaatggt tttacaggta gcacaaacca ttaagttatg caagtacaat
    13081 ttataaattc agaaaataca caaattcaaa aatgtattga aggttgcaga aaaagccaaa
    13141 aagagttgta tgatttctat gcagctaaaa tgtttgcagt ttgtttaaga tatgctaaaa
    13201 acgatatgga tgctgaagac atattacaag atggcttcgt gaaaatattt accaacttag
    13261 ataaataccg tgcagatggc tcttttgaag gttggttaag aagaattatg gtaaatactg
    13321 ctattgagca tattcgtaag aataaaataa ataatcaaga tatatctgaa aatatagaaa
    13381 atacagttaa agacaaatat gccaatgcat tagacaaatt gtttgaaaag gaactggtaa
    13441 atatagcatc tactttaagc gatggataca aagctgtttt taacatgtat gctattgaag
    13501 gctattcgca taaagaaatt gccaaacaat taggaataac agaaagtacc agtaaatctc
    13561 aattcagcag agcaaaatct atattaagaa atttattaca agtaaataat aatcaaaatg
    13621 ttatagcttg ttaattatta ttaataactc attaaaaaag cctcatttaa aactaaatga
    13681 ggctttttta gcaatataca caaaaagtta ctatattaat attaaacaac aatgtgatta
    13741 taagataatt agaacatact aattactata aattattagg tttattttat ctcctctccc
    13801 tcctctcttg gtatttctat ggttacttta tataaagatt gagaactatc tttctcatat
    13861 aaaatatcac catgaataac ccttaatcta ctttgtatat tctttaaacc taaaccttct
    13921 ttttcgttgg tatatcttaa aaattcttct tgggttaatc caaatccatc gtggtgcaat
    13981 cttaaaatta ctttttcttc aacatcgtgt tgggttaaat gtataaatga agctttacta
    14041 tgtttaagta cattatttac taactcttga ataactctaa ataaaataag ttctttatct
    14101 gattttagcc ttgttttata atcgtgaaat tttgaactag catttaaaga acctgaatta
    14161 cttatttttt gaaaaaaatc gttcacagca ctacttaaac caaaattttt taaagttggt
    14221 ggcattaaac tgtgagaaat gtttctaatt aattgaatag tatcatctat aatttgttta
    14281 gcattaaata aagaatgcat ttgtgcagtt ttatctaaat taacaaaatt atcgttgagg
    14341 tataattttg ctgttgctaa taatgggcct gcatcatcat gtaaatctgc tgctaacctg
    14401 gttctttctt cttcctgtaa ttttatagaa gcttttaata acattttctg atgctctact
    14461 tccatttgtt gtattctctg ctgaaaacgc attaccctgc gttgatgaaa aatagcaaat
    14521 aatattattg atgtagcaag taatatcatt cctattgttc ctaaaaataa aacaggggtg
    14581 atttgtgtag ttaaaagtat ttgagtaaaa tttattagta attgcataaa ttattagtct
    14641 tttcctaagt tatcttttat ataataaata ctatttttat tggggtgctc atttttttga
    14701 ttagcggggt taatgcaaaa tgcaatagtt attaaaatat gttttagaat atttccaccc
    14761 cttgataaat attcatatac aattgtatca aagtctcttt cttctggaga cacagaactt
    14821 aaaaagccaa aaaaagtacc ggcacaatac attaataaag ctgctacaat ccaaaattgg
    14881 tatgtgttat ataaaatttc tcctcttaca tttttaactc tttgaaacaa atacaacaaa
    14941 caacaaataa gtaataacaa aaattcaata gcttgagatt gataatcaaa aatttctata
    15001 ctctcaacaa aaatattctg cactaaccaa tacaataaaa agccagcata acataaccaa
    15061 ataaaaattt gttgcttttt gagtgagata attaaggtaa aaaagtaact gaggaaagca
    15121 aactcaataa cagaaaatat gttgataatc caagttgttt taatattatt tcttgctaag
    15181 ttataacacc aaatatcagt tattagactg attaccaata ataaaaaaat tacccatttt
    15241 gctctttgct tactgttaaa tttaaacagc acaaaaaaca aaatgggtat taaaggagag
    15301 aaaactgaaa tatgaaataa atagttcaat gctaaacaat ttgctcaaaa ataagcaaat
    15361 aatgtttaca taataaacct ttatataatt aagatgacca atctaaatcc atatcacctt
    15421 gtccacaact tggagggcaa attgcacttc tatccccaac aattccttta tgttttatta
    15481 catctttttt aattttttgt aaactttcca ccttcttctt gggaggctat ccaaccttta
//
LOCUS       DAOQHP010000008        15448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1827_length_15448_cov_4.059508, whole genome shotgun sequence.
ACCESSION   DAOQHP010000008 DAOQHP010000000
VERSION     DAOQHP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1827_length_15448_cov_4.059508"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /locus_tag="PLY81_00625"
     CDS             <1..2373
                     /locus_tag="PLY81_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNF28763.1"
                     /translation="SRDLSNFKVEMLKDEDIIKYKAYLQNAGITETQAEQMALQRGKP
                     SSEILKLKARVASLSSRTTTTTKPTTKGRTNTSRDIDSLDVEGSRVVEKEKVLKPEDE
                     IFGSALFNNNDLRFEPDLRIATPKNYIIGPDDEIAIDVYGYQETNPRLTVSPEGFINI
                     PNVGFVSVNGLTVETAAKRIKEKMIKNGYSSAATGNTKIEVSISKIRTIRVTVIGEAK
                     KPGTYNVSSLSSLFNVLYAAGGPSNKGSFRTIELIRNNKVIATLDAYDFLLKGIQQNN
                     VKLTDQDVVRIPAANILVKLKGEVKHQGIFEMLPTENMNSLLSFAQGFTGKAYTASVN
                     VKSFTDIERKIQDINKTEFINYKPKNGDEVFVGRVLERYSNRVILEGAVYRPGQYELT
                     SNLTLSKLIAKADGLKEDAYKERGLLYRTNEDLTKQVIDFNLNDVNSNNNQDIILQKD
                     DSIVIASAKDFYETYTLTVDGEVRKPGVYEYYKGITLKGLIFQTGGFTDAATAQRIEI
                     ARRIKSDDINATAIAQVIEIATEKDLSIKGEEVKLQPWDVVIVRSNPGYKPQIAVKVE
                     GEVLYPGTYILGSKEDKVSDIIKRAGGVTPHSDKTGAFITRINTSAFKDEAAERLQKL
                     KKASDTSTQLIEELTKPTVKISLRLDEVLNNKGNLLENITLMEGDVIHVPKQRNVVKV
                     NGEVMFPTEIVYKEGASIHYYIDKAGGFTENARKKKVTVLNSNGSAAKTKKFLFFRSY
                     PSVLPGSEILVPKIPVKENKGLSTAEWLAVASGLASLAGVAVAIINVSRK"
     gene            2400..3452
                     /locus_tag="PLY81_00630"
     CDS             2400..3452
                     /locus_tag="PLY81_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28764.1"
                     /translation="MSLVNPEEKVVKAKHLIALMKYWWKFLYAKKVLIIAITFLGAVV
                     GFTFGWFQKPNYKAELTFAPEDAGQLGGYLGIAAQFGIDLGMGSGNVFEGENITELFK
                     SSTLISNTLYSEATIGGKNQTLIAYKIASKYKKNKPVLDSLQLHQVNFNQSLQNKALG
                     IRFADSLVKAIIKETSKSLDVNKVDKKLNIIKVSFTDKDEVFAKLFVDLLAQNAIKYY
                     TGYKSKKARDNVEILQHQADSVKRMLFGGIGDVASMNDLNVNPTKQTARVSTQKRTID
                     VQVNGAIYTEILKNLELAKIALRKETPFIQIIDRPYYPLERSRIGRLLGAILGTFIFG
                     FGIVFFLVIKKSFKEE"
     gene            3523..4722
                     /locus_tag="PLY81_00635"
     CDS             3523..4722
                     /locus_tag="PLY81_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF28765.1"
                     /translation="MFIGHSFFAFSQWIQLSVVTKFCTSLDLGIYTLGLAIIGPLFIF
                     AGLQLKAIQITDVTTNWKFQEYFTLRVICLLAAIIFVIVYSLLFSREFFSLFLLLALL
                     KVVEGISEIMNGQQQLQEDMKKVAISNILRGFAIAVGVFIGVYFIKSLVVGLLIALLG
                     NLLVLVFYDYKNCTTLLKTKQLIQFATTRYKSIVLKALPLAIVVTLIYLNANVAKYFI
                     EYLKSTVEQGIYSTIGYLIVIGNLVNNALGQAITPRLSKYFAEREMKKFNQLAKQFII
                     ANIVIGILIIAVVHFTGEYILLFFFNNSIAQYTQLFTLFMVAGLLLYCVSCIGYILTS
                     MHYIKQQPIIHIVGLATNLICCFLLIRIYGLNGAAYAWIISFAAQLIISVLLLNKSYK
                     NILKPAV"
     gene            4726..5796
                     /locus_tag="PLY81_00640"
     CDS             4726..5796
                     /locus_tag="PLY81_00640"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HNF28766.1"
                     /translation="MHFAFSQYFIYIVFVAVITAFSYINKLNKTDVFAYLMYSFMLLM
                     AVLRIDTGADYATYIEMHERVGKSGFQDMLDFTEPLFFWLLKLIYVFTDDYWIMFAVV
                     SFFIYTVLFLALKKLSTDIALSLILYVITSYYFISINQLRQSISLVIFLYSLTYIINA
                     KPGKYFLLNLIGVGFHYSAAIVLFLYPLFRINFSRRNLIIIYLASIVLSIFIEKMFVS
                     SLSALDLFYAQYFDMEEYMEKNYLALGKVVIPNLILIYSIIQFKNFSKAEYSIFLVFF
                     ASIIYYNIFFGKHIFIRPGMYFEIVTILYIPIFIKKFFSITMGLVVETFLIIYYFFAV
                     TLYGILINGGQGVVPYKFIFSR"
     gene            5793..6875
                     /locus_tag="PLY81_00645"
     CDS             5793..6875
                     /locus_tag="PLY81_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF28767.1"
                     /translation="MRIVHIAYFDFEYKTGIGEVVVNLAKAQQQNGNDVFILNTLSQN
                     NIENYENIGVNIVTNTFNFKNYIQTIKPNVVVFYSFYQLPFLKFYKILKALKIPYFIQ
                     PQGAFGKANQQKGYLKKKLANYLFFNAFIQNSNGILYLNNAEKSISVFNWFKASYILP
                     NGVFTNVNYLQNKTPNLPINLIFLSRIDLMHKGLDILLDALLKVDKQLAEKNISLQIY
                     GYGEEADVNYVKKRIPDFTCNINLNNPVFGNEKENVLCNADIFVLTSRYEGMPVAILE
                     ALAYGVPCFITPGTNMAEIIKENNAGWVTSLNTDEIGVNLLNAIEEYKINFSTLRNNA
                     RNCAVNNYQWSVVAEKSIQIYQQAVS"
     gene            6909..7760
                     /locus_tag="PLY81_00650"
     CDS             6909..7760
                     /locus_tag="PLY81_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF28768.1"
                     /translation="MTNTTHNQYEPFSVLMATYYKDNPQFLRAALNSLFTQTVLPSEI
                     VLVIDGSIPNSIQEVINDYLKQQPTIFKTFPLDKNVGLGNALNYGLKMCNNELVARMD
                     SDDICVHNRFEIQLNYFKNNPTVSAHGGWMNEFEIELNDRNKIKSTPIGLDKIIKYAR
                     LRNPLNHPTVMFKKSAVLSVNSYIEINLFEDFYLWLRMLNKGLLLANSKDILVNFRVN
                     DSMIARRHGLNYLKKELQFIIKCYRENLIPFNSLLVQILTRLPLRLMPMGALKFIYKY
                     ILRNNSN"
     gene            7769..8470
                     /locus_tag="PLY81_00655"
     CDS             7769..8470
                     /locus_tag="PLY81_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF28769.1"
                     /translation="MQAIILAGGLGTRLKSVVANKPKVLSPVAGKPFLHYIITYLQNQ
                     GVEKYIFALGYLSEQVIDFLKDEYPSLNYEYTIEDTPLGTGGGIRKAIQLITEENVLI
                     VNADTFFEVDIKALHSFHLSTNAHCTISLKQMYNFERYGCVELDNNSNTILSFKEKTF
                     TTKGYINAGYLVINKNYFLSATNNLPQIFSFEKDFLEKNLAKMLIKGLAFDGYFIDIG
                     IPEDFVLAQEKFKNI"
     gene            8572..9543
                     /locus_tag="PLY81_00660"
     CDS             8572..9543
                     /locus_tag="PLY81_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF28770.1"
                     /translation="MNVCVIGGSGFIGSHLINSIATHHNVTNVDKQLPEYNNVNVANV
                     IADIRDYDALKLAIPDNSDYIILLAAEHRDDVSPISLYYDVNVEGTHNVLRVMAEKNI
                     EHIIFTSSVSVYGLNKKDPDELSGTDPFNHYGKSKLEAEKVLRRWFDNDRNGKSLIIV
                     RPTVTFGPGNKGNVYNLLKQIASGNFIMVGKGRNKKSMAYVENVAGFISYCLDKKFKG
                     YHLFNYIDKPDLSTKELVHIAEEALGKKLPPIRIPYFIGYTGGLTLDLISRITKKKFP
                     ISAVRVKKFCATTQFSSATIQATNYTPKKQLSEGLSITIKSIVNGSK"
     gene            9590..10699
                     /gene="gmd"
                     /locus_tag="PLY81_00665"
     CDS             9590..10699
                     /gene="gmd"
                     /locus_tag="PLY81_00665"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF28771.1"
                     /translation="MKKALITGITGQDGAYLAEFLLKKGYEVHGIKRRSSLFNTQRID
                     HFYEDPHIPNRHLTLHFGDLTDSTNLIRIIQEVQPDEIYNLAAMSHVHVSFETPEYTA
                     NADGIGTLRILEAVRLLGLINKTKIYQASTSELYGLVQAVPQSETTPFYPRSPYAAAK
                     LYAYWITVNYREAYNMYACNGILFNHESPLRGETFVTRKITRAVAAIALGFQEQLFLG
                     NLNAQRDWGHAKDYVEAMWLILQQEKPEDFVIATGKTTYIRDFVKMAFAEIGIELEFN
                     GEAENEEGKVIANTGKYNIPIGKIVVKVDKRYYRPTEVDLLIGDPTKAQTKLGWKPKY
                     TLAEMVKEMVANDIIVYSKERMLKDNGFSTKSLSQ"
     gene            10706..11638
                     /locus_tag="PLY81_00670"
     CDS             10706..11638
                     /locus_tag="PLY81_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNF28772.1"
                     /translation="MKKDSKIYIAGHRGMVGSAIVRNLQQKGFSNIIYKTSNELDLRN
                     QQAVNDFFEKEKPQFVFLAAAKVGGIVANNTYRADFIYENLMIEANIIHAAFINKVEK
                     LLFLGSSCIYPKLAPQPLKEEYLLSGYLEETNQPYALAKIAGIELCNSYRRQYDCNFI
                     SAMPTNLYGPNDNYDLNNSHVLPALLRKFITAKKQNDSFVELWGTGTPKREFLHVDDL
                     AEACYFLMLHYNEEGLVNVGVGEDLTILELAQLIKKITGYTGEIKLNTSKPDGTPRKL
                     LDVTKINQLGWKANITLEQGIQKINEEVKDNDWK"
     gene            11675..12871
                     /locus_tag="PLY81_00675"
     CDS             11675..12871
                     /locus_tag="PLY81_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF28773.1"
                     /translation="MTKPNLIIILNRLVIGGQAVDTIPLAYYLQQYFTVIILYGEKEA
                     DEVEASFLLHQYPGLQLIKIKQLRRTINPLTDFFAFWSIVQQIKKYNTKIVHTHGSKS
                     GLLGRLAAKFLGVKVIVHTFHGHLFHSYFNSLGTWLLLQTERALAKISTHIIALSYKQ
                     KNELLQVYEIADESKIHVVPLGIDEQKFVQQDLSKRENFRTKFHLNENDIAVGIIGRI
                     VPIKNHQLFIKIAEKVIANSQYQNIYFFIVGDGAGMQNLMKDLDKKNITYSPKNNNSR
                     IIFTSWVEDMIWAYYGLDIVMLTSLNEGTPLSIIEAQFCGKPVIAVNVGGVKDTFDSE
                     KSGFLINDYKEESFIQPLYQLADNKSLIQQMAKEAVDFATHNFTKQKEVEAIKNIYLK
                     QLEKLN"
     gene            12871..13923
                     /locus_tag="PLY81_00680"
     CDS             12871..13923
                     /locus_tag="PLY81_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNF28774.1"
                     /translation="MKILITGTAGFIGFHVAEKLVQQNHTIVGIDSINNYYDVTLKSA
                     RLSEAGFDANAIAYNSKVVSSKHPNYSFIQLQLEDAASLQMLFTIEKFDIVINLAAQA
                     GVRYSLTHPAAYINSNITGFANLLECCRHFNIQHLVYASSSSVYGLNEKVPFAVTDST
                     NHPASLYAATKKSNELMAHVYSHLFNLPTTGLRFFTVYGPWGRPDMAPFLFTEAILHH
                     KPIKVFNHGDMMRDFTYIDDVVELISRVIEKPASINKNWDAQNPDAATSSAAYKIYNI
                     GNHEPVALNDFIKAIENATGKKATKEMLPIQPGDVQKTFADIKELEQDFNFKPYTPLQ
                     KGIDKFVTWYKNYYKI"
     gene            13932..14990
                     /gene="rfbB"
                     /locus_tag="PLY81_00685"
     CDS             13932..14990
                     /gene="rfbB"
                     /locus_tag="PLY81_00685"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNF28775.1"
                     /translation="MKKNILITGGAGFIGSHVVRLFVTKYPAYNIINLDALTYAGNLE
                     NLKDIDQHKNYFFEKVDILDAKNLKNIFQKHQVTDVIHLAAESHVDRSILSPLDFVYT
                     NVIGTVNLLNTAKDAWKEDFTKHLFYHVSTDEVYGALGAEGFFTETTSYHPNSPYSAS
                     KASSDHFVRAYAETYHLQTIISNCSNNYGPNHFPEKLIPLFINNIINQKPLPVYGDGL
                     YTRDWLFVKDHATAIDMVFHHGKINDTYNIGGFNEWKNIDLVKILCKLMDEKLKRNAG
                     ESEQLITYVKDRPGHDRRYAIDATKINQELGWKPSVTFEEGLSLTIDWYLQNTEWLNN
                     VTNGTYQSYYNEMYAYRG"
     gene            15204..>15448
                     /locus_tag="PLY81_00690"
     CDS             15204..>15448
                     /locus_tag="PLY81_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28776.1"
                     /translation="MRKILFTITFLIITQNVFSQNIFSFLFKGEKEISISTPLLWSQY
                     EVTNTFGIYGADKKSNGESWSMGLNATYSKPIYKNFY"
BASE COUNT         5727 a         2074 c         2514 g         5133 t
ORIGIN      
        1 agccgagatt tatccaactt taaagtagag atgctaaaag atgaagacat tataaaatat
       61 aaagcctatt tacaaaatgc aggtattaca gaaacacaag cagagcaaat ggctttacaa
      121 agaggtaagc catcttcaga aattttaaaa ttaaaagcaa gagttgcaag tttatctagc
      181 agaactacta ctaccacaaa gccaactacc aaaggaagaa ctaatacgag tagagatata
      241 gactctttag atgtagaggg tagtagagta gttgaaaaag aaaaagtttt aaaacccgaa
      301 gatgaaattt ttggttccgc tttatttaat aataatgatt tgcgttttga acctgattta
      361 agaattgcca ctcctaaaaa ttatattata ggcccagatg atgaaattgc aattgatgtg
      421 tatggatatc aagaaaccaa tccaagactt acagtatctc ctgaaggatt tataaatata
      481 cccaatgtag gttttgtttc agtaaatggt ttaactgtag aaactgctgc aaaaaggatt
      541 aaagaaaaaa tgattaaaaa tgggtattca agtgcagcaa cgggcaacac taaaattgaa
      601 gtaagcatta gtaaaattag aactattaga gttactgtaa ttggagaagc taaaaaacct
      661 ggtacctata atgtgtcttc tctatcatcc ttatttaatg tgttgtatgc agcaggtggc
      721 ccaagtaata aaggtagttt tagaaccata gaattaataa gaaataataa agtaatagcc
      781 accttagatg cttacgattt tctattaaaa ggcattcaac aaaataatgt taaactaacc
      841 gatcaggatg ttgttagaat accagcagcc aatattttag taaagcttaa aggagaagta
      901 aagcaccaag gaatttttga aatgttgcca accgaaaata tgaatagctt attaagcttt
      961 gcacaaggct ttacaggtaa agcttataca gcatcggtta atgttaagtc gtttacggat
     1021 attgaaagaa aaattcaaga cattaataaa acagaattta taaattataa acccaaaaat
     1081 ggagatgaag tttttgtagg aagagtttta gaaaggtata gtaacagagt aattttagaa
     1141 ggtgcagtat atcgtcctgg acaatatgaa ctaacttcca accttacatt aagtaaacta
     1201 atagcaaaag cagatggctt aaaagaagat gcttataaag aaagaggatt gttatataga
     1261 acaaacgaag acttaacaaa acaagtaata gactttaatt tgaatgatgt taatagcaat
     1321 aataatcaag atattatttt gcaaaaagat gattcaattg taattgcttc ggccaaagat
     1381 ttttatgaaa cctatacttt aacagtagat ggagaagtaa gaaaaccagg tgtgtacgaa
     1441 tactataaag gcattacttt aaaagggtta atatttcaaa caggaggctt taccgatgct
     1501 gctacagcac aaagaataga aattgcaaga agaattaaaa gcgatgatat taatgccact
     1561 gcaatagccc aggttataga aattgcaacc gaaaaagatt taagtattaa aggcgaagaa
     1621 gtaaaattgc aaccttggga tgtagttatt gtaagaagta atccaggcta taaacctcaa
     1681 attgctgtaa aagtagaagg agaagtttta tatccaggaa cctatatttt agggtctaaa
     1741 gaagataaag taagcgatat cataaaacgt gcaggtggcg taacaccaca ctccgataaa
     1801 acaggagcat ttataactcg tattaatacc agtgcattta aagatgaagc tgccgaaagg
     1861 ttacaaaaac taaaaaaggc aagcgatact tctactcaac ttattgaaga attaactaaa
     1921 ccaacagtaa aaattagttt aagattagat gaagtattaa ataataaagg caacttatta
     1981 gaaaatatta ctttaatgga aggcgatgta atacatgtac ctaaacaaag aaatgtagtg
     2041 aaggtaaatg gagaagtaat gtttcctaca gaaattgtgt ataaagaagg agcaagcata
     2101 cactattata ttgataaagc aggaggcttt acagaaaatg ccagaaagaa aaaagtaacg
     2161 gtgttaaact ccaatggtag tgcagctaaa acaaagaaat ttttattttt cagaagttac
     2221 ccaagtgtat tgccaggttc agaaatatta gttccaaaaa ttccggtaaa agaaaataaa
     2281 ggattaagca ctgcagaatg gttggctgtt gcaagtggtt tagcatcttt ggcaggtgtt
     2341 gcagttgcaa ttataaatgt aagtagaaaa taattttttt ttaataaatt ttattatttg
     2401 tgagtttagt aaaccctgaa gaaaaagttg taaaagcaaa acacctaatt gcactcatga
     2461 aatattggtg gaagttttta tatgctaaaa aagtattgat aatagctatt acatttttgg
     2521 gtgcagtagt aggttttact tttggatggt ttcaaaaacc aaattataaa gcagaattaa
     2581 cttttgcacc agaagatgct ggccaacttg gaggttattt aggaattgct gcacaatttg
     2641 gcattgattt aggcatgggc agtggtaatg tttttgaagg agaaaatatt accgaattat
     2701 ttaaaagcag cactttaatt tcaaatacac tttattcaga agctaccata ggcggtaaaa
     2761 atcaaacctt aattgcttat aaaattgctt ctaaatacaa aaaaaataag cctgttttag
     2821 attcgttaca attacaccaa gttaatttca atcaatctct acaaaacaaa gctttaggaa
     2881 ttaggtttgc cgatagttta gtgaaagcaa ttattaaaga aacatccaaa tccttagatg
     2941 ttaataaagt tgataaaaaa ctaaacatca ttaaagttag ttttacggat aaagatgaag
     3001 tttttgcaaa attatttgtg gatttattag cacaaaatgc aataaaatat tatacagggt
     3061 ataaaagtaa aaaggcaaga gataatgttg aaatcttaca gcatcaggcc gactctgtta
     3121 aaagaatgtt gtttggagga ataggagatg ttgcaagtat gaatgatttg aatgtaaatc
     3181 ctactaaaca aactgcaagg gtaagtactc aaaaaagaac tattgacgta caagtgaatg
     3241 gtgcaattta tacagaaatt cttaaaaatt tagagttagc taaaatagct ttaagaaaag
     3301 aaacaccttt tattcaaatt attgatagac cttattaccc attagaaaga agcagaattg
     3361 gtaggttatt aggtgccatt ttaggtacat tcatttttgg ttttggtatt gtattcttct
     3421 tagttattaa aaaatctttt aaggaagaat aacagagtaa attttatttt ctaaaatttc
     3481 atttttattt ctacaaaaag cattaagcat agcatatcat ggatgtttat agggcattca
     3541 ttttttgcat tttcacagtg gatacaatta tcagtagtta ctaaattttg tacctcgtta
     3601 gatttaggta tttatacatt gggcttagct attataggcc ctctttttat ttttgcaggg
     3661 cttcaactaa aagcaattca aataacggat gtaacaacaa attggaaatt tcaagagtac
     3721 tttacattac gtgttatttg cctattggct gcaattattt ttgttattgt ctatagctta
     3781 cttttttcaa gagaattttt ttcacttttt ttattacttg cattattaaa agttgtagaa
     3841 ggtatttctg aaattatgaa tggccagcaa cagctacaag aagatatgaa aaaagtagct
     3901 atttcaaata tattaagagg ttttgctatt gcagtggggg tgtttattgg cgtttacttc
     3961 attaaaagtt tagtagtagg gcttttaatt gcattactcg gtaaccttct tgtacttgtt
     4021 ttttacgatt ataaaaactg cactacttta cttaaaacta aacaacttat tcaatttgca
     4081 acaacacgtt ataaaagtat tgtattaaag gcattgccat tagctattgt agttacccta
     4141 atttatttaa atgctaatgt ggctaaatat tttattgaat atttaaaaag tactgttgaa
     4201 caaggtattt attctacaat tggttactta attgtaattg gtaatttagt taacaatgct
     4261 ttagggcagg caattacacc aaggttaagt aaatattttg cagaaagaga aatgaagaag
     4321 tttaaccaac ttgcaaagca gtttataatt gcaaatattg taattggtat cttaattatt
     4381 gcagtggttc attttactgg agaatatatt ttattatttt tttttaataa ttcaattgca
     4441 caatacaccc aactatttac attgtttatg gttgcaggtt tactgcttta ttgtgtttct
     4501 tgtattggat atattttaac cagtatgcat tatattaagc aacaaccaat aattcatatt
     4561 gttggcttag ccacaaattt aatttgttgt tttttactaa ttcgtattta tggtttaaat
     4621 ggtgcagctt atgcctggat tatttccttt gctgcacaac taattattag tgttctttta
     4681 ttaaataaat cgtataaaaa tattttaaag cctgctgttt aagtaatgca ctttgctttt
     4741 tctcaatatt ttatttatat agtttttgtg gctgtaatta cagccttctc ttatattaat
     4801 aaattaaata aaacagatgt ttttgcatac ttaatgtatt cctttatgct gttaatggct
     4861 gtattaagaa ttgatacagg agcagattat gcaacgtata ttgaaatgca tgaaagagtt
     4921 ggcaaaagtg gctttcaaga tatgttggat tttacagaac cacttttttt ctggttactt
     4981 aaattaattt atgtatttac cgatgattat tggataatgt ttgctgttgt atcttttttt
     5041 atatacacag ttttattttt agcattaaaa aaactttcta cagatattgc cttatcgtta
     5101 atactatatg ttattacttc ttactatttt atttctatca atcagttaag gcaatccata
     5161 tctttagtaa tttttttgta cagtttaact tatataataa atgccaagcc aggaaaatat
     5221 ttcttgctaa atttaattgg tgtaggcttt cactattcag cagccattgt attatttcta
     5281 tatcccttat ttagaattaa tttttctcgt agaaatttaa ttatcattta cttggcatct
     5341 attgtacttt ctatttttat cgaaaaaatg tttgtatcct ctctatcggc tttagattta
     5401 ttttatgccc agtattttga tatggaagaa tacatggaaa aaaattattt agctttaggc
     5461 aaagtagtta ttccaaattt aattttaatt tattccatta tacagtttaa aaactttagc
     5521 aaagccgagt actccatttt tttagtattc tttgcaagta ttatctatta caatatattt
     5581 tttggcaaac acatttttat caggccagga atgtattttg aaattgtaac aatattgtat
     5641 attccaatat ttataaaaaa attctttagt ataacaatgg gtttggttgt agaaacattt
     5701 ttaattattt attacttctt tgctgtaaca ctctatggta ttttaattaa tggtgggcaa
     5761 ggagtagtgc cttataaatt tattttttcc agatgagaat tgtacatatt gcttattttg
     5821 actttgaata taaaacagga attggagaag tagttgtaaa tcttgctaaa gcacaacaac
     5881 aaaatggaaa tgatgtattt attttaaata ccctatctca aaataatata gaaaattacg
     5941 aaaatattgg agttaatatt gttactaata cttttaattt taaaaattat attcaaacaa
     6001 ttaagccaaa tgttgtagtt ttttattctt tttatcaact gcctttttta aagttttata
     6061 aaattttaaa agcattaaaa attccatatt ttatacagcc tcaaggtgcc tttggcaagg
     6121 ctaaccaaca aaagggttat ttaaaaaaga aacttgctaa ttacttattt tttaatgcat
     6181 ttatacaaaa ctcaaatggt attttatact tgaataatgc agaaaaaagt atttctgttt
     6241 ttaattggtt taaagcttct tatattttac caaatggtgt ttttactaat gtaaattatt
     6301 tgcaaaataa aactccaaat ttgccaatta accttatttt tttatctaga atagatttaa
     6361 tgcataaggg tttagacata ttgttagatg ctttattaaa agtagataaa caacttgctg
     6421 aaaaaaatat cagtttgcaa atttatggtt atggagagga agctgatgta aattatgtaa
     6481 aaaaaagaat tccagatttt acttgcaata tcaatcttaa taaccctgtt tttggtaatg
     6541 aaaaagaaaa tgttttatgc aatgcagaca tttttgtttt aacatcaaga tatgaaggca
     6601 tgcctgttgc aattttagaa gcattagcgt atggagtgcc ttgttttatt acaccaggta
     6661 ccaatatggc agaaataatt aaagaaaaca atgctggttg ggtaacttca ttaaatacag
     6721 atgaaatagg agtcaatctt ttaaatgcaa ttgaggagta taaaataaat ttttcaaccc
     6781 taagaaataa tgctagaaac tgtgctgtta ataattacca gtggagtgtt gtagcagaaa
     6841 aatcaattca aatttaccaa caagcagtta gttagaaagc aaattaaccc ttaacctccc
     6901 tttcttaaat gactaataca acccataatc aatacgagcc attttctgtg ttaatggcca
     6961 cttattataa agataatcca caatttttaa gagccgcatt aaatagtttg tttacacaaa
     7021 cagtacttcc atcagaaatt gttttagtaa tagatggtag cattccaaac tctattcaag
     7081 aggttattaa tgattatctt aaacaacaac caacaatttt taaaaccttt cctttagata
     7141 aaaatgttgg tttgggcaat gcattaaatt atggcttaaa aatgtgtaac aatgaattag
     7201 tggctagaat ggatagtgat gatatttgtg tacataaccg atttgaaatt cagttgaatt
     7261 attttaaaaa caatccaact gtatctgcac atggtggctg gatgaatgaa tttgaaattg
     7321 aattaaacga taggaataaa attaaatcaa ccccaattgg tttagataaa attataaaat
     7381 atgcaaggct aagaaaccca ctcaatcacc caacagtaat gtttaaaaaa tctgctgtac
     7441 ttagtgtaaa ttcttatatt gaaattaatt tgtttgaaga tttttattta tggttaagga
     7501 tgttaaataa aggccttcta ttagctaact caaaagatat tttagtaaac tttagagtaa
     7561 acgatagcat gattgccaga agacatgggc ttaactactt aaaaaaagaa ttacagttta
     7621 ttattaaatg ctacagagaa aatttaattc cttttaattc attacttgta caaatattaa
     7681 cacgtttgcc tttaaggcta atgcctatgg gggctttaaa gtttatttat aaatacattt
     7741 taagaaataa ttcaaactaa agtttacaat gcaagccatt attctcgcag gaggtttagg
     7801 tactcgttta aaatcggtag tggcaaataa gccaaaagta ttatctccag ttgctggtaa
     7861 acctttcttg cattatatca ttacttattt acaaaaccaa ggcgttgaaa aatatatttt
     7921 tgctttagga tatttgagtg agcaggtaat tgatttttta aaagatgaat acccctcatt
     7981 aaattatgaa tatacaatag aagatacacc attaggcaca ggtggaggaa ttagaaaagc
     8041 aattcaatta ataactgaag aaaatgtttt aattgtaaat gccgatactt tttttgaggt
     8101 ggatattaag gctctgcact cttttcatct ttctaccaat gcacattgta ctatatcttt
     8161 aaaacaaatg tataattttg aaaggtatgg ttgtgttgag ttagataata actctaacac
     8221 aattctttct tttaaagaaa aaacttttac aactaaagga tatattaacg cagggtattt
     8281 agtaataaat aaaaattatt ttttgtcggc aaccaataat ttaccccaaa tattttcttt
     8341 tgaaaaagat tttttagaaa aaaatttggc taaaatgctc atcaaagggt tagcttttga
     8401 tggctatttt atagatattg gaataccaga agattttgta ctggctcagg agaaatttaa
     8461 aaacatatag ttaagctaag aaatttaata acaaatcaac ccaataagaa attttgttga
     8521 tttttgtata gtgcaattcc ttatcttgct tttttaattt taggataata aatgaatgtt
     8581 tgtgtaattg gaggctcagg atttattggc tcgcatctaa ttaattctat tgcaacccac
     8641 cataatgtaa ctaacgttga taagcaacta ccagaataca ataatgttaa tgttgcaaat
     8701 gttattgcag atataagaga ttatgatgca ttaaaacttg ctatacccga taattctgat
     8761 tacataattt tattagcagc cgaacataga gatgatgttt caccaatttc tctttattat
     8821 gatgtaaatg tagaaggtac gcataatgtg ttaagagtaa tggctgaaaa aaatattgaa
     8881 catattattt ttaccagttc tgtttctgta tatggtttaa ataaaaaaga ccccgatgaa
     8941 ctttctggca ccgacccttt taaccattat ggtaaaagta aattagaagc tgaaaaagtt
     9001 ttaaggcgtt ggtttgataa tgatagaaat ggtaaatcct taatcattgt taggcctaca
     9061 gtaacctttg gtccaggtaa taaaggaaat gtttacaacc ttttaaaaca aattgctagt
     9121 ggtaatttta ttatggttgg caaaggccga aacaaaaaat caatggctta tgtagaaaat
     9181 gttgctggtt tcatcagtta ttgtttagat aaaaaattta aaggctatca tttatttaat
     9241 tatatagata aaccagattt atctactaaa gaattagtgc atattgccga agaagcttta
     9301 ggcaaaaaat taccgcctat tagaattcct tattttattg gctacacagg tggcttaaca
     9361 ttagatttga tttctagaat taccaagaaa aaatttccta ttagtgcagt tagagttaaa
     9421 aagttttgtg ctactacaca attttcttct gccactattc aggctaccaa ctacacccct
     9481 aaaaaacaat tatccgaagg gttatcaatt actattaaaa gtattgtaaa cggttcaaaa
     9541 taatacttaa ttagctatta ttaaaggtta gtataaattt aatttagaaa tgaagaaagc
     9601 attaattact ggcattacag ggcaagatgg tgcatacctt gccgagtttt tattaaagaa
     9661 aggttacgaa gtacatggca ttaaacgcag aagttcgtta tttaatacac aacgtataga
     9721 tcatttttac gaagatccac atattcctaa tcgtcattta acattgcatt ttggcgattt
     9781 aacagacagc accaatttaa taagaattat acaagaagta cagccagatg aaatttataa
     9841 tttggcagca atgagccatg tgcatgtaag ttttgaaaca ccagaatata cagctaatgc
     9901 agatggtata ggcactttaa gaattttaga ggctgtaaga ttattaggtt taataaataa
     9961 aacaaaaata taccaagcct caacttctga attgtatggt ttagtgcaag cagtacctca
    10021 aagcgaaaca actccattct atcctcgtag cccttatgct gcagcaaaat tgtatgctta
    10081 ctggattaca gtaaactaca gagaagctta taatatgtat gcttgcaatg gaattttatt
    10141 caaccatgaa tctcctttac gtggcgaaac ttttgtaaca agaaaaatta ctagagcagt
    10201 agctgcaata gctttaggtt ttcaagaaca attattttta ggtaacttaa atgctcaaag
    10261 agattggggg catgcaaaag attatgtaga agctatgtgg cttattttac aacaagagaa
    10321 gccagaagat tttgtaattg caacaggaaa aacaacttac ataagagatt ttgtaaaaat
    10381 ggcttttgcc gaaataggca ttgaattaga atttaatggc gaagctgaaa atgaagaagg
    10441 taaagtaatt gctaatactg gtaaatacaa tattcctatt ggaaaaattg tagttaaagt
    10501 agataaaaga tattataggc ctacagaagt agatttatta ataggagatc ctacaaaagc
    10561 tcaaacaaaa ttgggttgga aacctaaata tactttagca gaaatggtaa aagaaatggt
    10621 agccaacgat attattgttt actcaaagga aagaatgtta aaagataatg gcttctccac
    10681 taaaagttta tcgcaataaa gtaatatgaa gaaagattct aaaatttata ttgctgggca
    10741 tcgtggaatg gtaggcagtg ctattgtgcg aaacttgcaa caaaaaggtt ttagtaatat
    10801 tatttacaaa acgtctaatg aattagattt aagaaaccaa caagcggtaa atgatttttt
    10861 tgaaaaagaa aaaccacaat tcgttttttt agctgctgca aaagttggtg gaattgttgc
    10921 caacaatacc tatagagccg attttattta tgaaaattta atgatagaag ccaatattat
    10981 tcatgcagct tttataaata aagtagaaaa attattattc ttaggctcat cttgtattta
    11041 tccaaaatta gctccacagc ctttaaaaga agaatacctt ttatctggtt atttagaaga
    11101 aaccaatcag ccttatgcct tagcaaaaat tgcaggaata gaattatgca acagttatag
    11161 aaggcagtac gattgcaatt ttatttcggc catgcctact aatttatatg gacctaatga
    11221 taattacgat ttaaataact ctcatgtttt accagctttg ttaagaaagt ttattacagc
    11281 taaaaaacaa aatgattctt ttgttgaact ttgggggacg ggcactccca aaagagaatt
    11341 tttgcatgta gatgatttag cagaagcttg ttatttttta atgctgcatt acaatgaaga
    11401 aggattggta aacgtaggag taggagagga tttaaccatt ttagaattag cacaactgat
    11461 aaaaaaaatt acaggctata caggagaaat aaaattaaat acctcaaaac ccgatggcac
    11521 tccaagaaaa ttattagacg taactaaaat taatcaattg ggctggaagg ctaacattac
    11581 tttagagcaa ggcattcaaa aaataaatga agaagtgaaa gacaatgatt ggaaataaaa
    11641 taaaacttac aacccctttt cttgcatttg taacatgacg aaacccaacc tcattattat
    11701 tctaaataga ttggttattg gtggtcaggc tgtagataca attcctttag cttactattt
    11761 acaacaatat tttactgtaa ttattttgta tggagaaaaa gaggctgatg aagtagaagc
    11821 ctctttttta ttgcaccaat atcctggcct gcaactaata aaaataaaac aacttagaag
    11881 aacaataaat cctttaacgg atttttttgc attttggagt attgtacagc aaataaaaaa
    11941 gtataataca aaaatagttc atacacatgg ctccaaaagt ggtttgttag gcagattagc
    12001 agcaaaattt ttaggtgtaa aagttattgt acatacattt catggtcatt tgtttcactc
    12061 ttattttaat tcgttaggta catggctgtt attgcaaaca gaaagagcac ttgctaaaat
    12121 ttcaacacat attattgcat taagttacaa acaaaaaaac gagttattac aagtatatga
    12181 aattgccgat gaatctaaaa ttcatgttgt tcccttaggt attgatgagc aaaaatttgt
    12241 acaacaagat ttatccaaac gagaaaattt cagaacaaaa tttcatttaa atgaaaatga
    12301 tatagcagta ggcattattg gtagaattgt tcctataaaa aaccatcagt tatttataaa
    12361 aattgcagaa aaagtaattg ctaatagtca atatcaaaac atttattttt ttattgtagg
    12421 cgatggtgca ggtatgcaaa acctgatgaa agatttggat aaaaaaaaca ttacttatag
    12481 ccctaaaaac aacaatagta gaataatttt tacctcttgg gttgaagata tgatttgggc
    12541 ttattatggt ttagatattg taatgttaac ctcattaaac gaaggcacgc ctttgtcaat
    12601 tattgaagct cagttttgtg gaaagccagt aatagcagta aatgtaggag gtgttaaaga
    12661 tacttttgat tctgaaaaat ctggcttttt aattaatgat tataaggaag aaagttttat
    12721 acaacctttg tatcaattag cagataacaa aagtcttatt cagcaaatgg ctaaagaagc
    12781 agttgatttt gctacacata attttacaaa acaaaaagaa gtggaagcaa taaaaaatat
    12841 ttatcttaaa caattagaaa aattgaacta atgaaaattc ttattacagg tacagcaggt
    12901 tttataggtt ttcatgttgc agaaaaatta gtacaacaaa atcataccat tgttggtatt
    12961 gatagtatta ataattatta tgacgttact ttaaaaagtg cccgattatc tgaagctggt
    13021 tttgatgcca atgcaatagc atataattca aaagtagtaa gcagcaaaca tcctaattat
    13081 tcctttatac aattacagtt agaagatgca gcttcactgc aaatgttgtt taccatagaa
    13141 aagtttgata ttgtaataaa ccttgcagca caagcaggtg tacgttatag tttaacacat
    13201 cctgcagcat atataaacag caatattaca ggctttgcca acttgctgga atgttgtcgt
    13261 catttcaaca ttcagcattt ggtatatgca agcagcagta gtgtgtatgg gctaaatgaa
    13321 aaagttccat ttgctgtaac cgattcaacc aatcatcctg catctttata tgctgctacc
    13381 aaaaaaagca atgaattaat ggctcatgtg tatagccatt tatttaattt acctacaaca
    13441 gggcttagat tttttacagt gtatggacct tggggcagac cagatatggc tcctttttta
    13501 tttactgaag ccatactaca tcataaacca attaaagtat ttaatcatgg agatatgatg
    13561 agagacttta catacattga tgatgtggta gaattaatta gtagagtaat tgaaaaacca
    13621 gcatcaataa ataaaaattg ggatgcacaa aatccagatg cagcaacatc ttcagcagct
    13681 tataaaattt acaatattgg caatcacgaa cctgttgcct taaacgattt tataaaagca
    13741 atagaaaatg caactggcaa aaaagctact aaagaaatgt taccaataca accaggcgat
    13801 gttcaaaaaa catttgccga tataaaagaa cttgaacaag attttaattt taaaccttat
    13861 actccattac aaaaaggaat agataaattt gtaacgtggt ataaaaatta ttacaaaatt
    13921 taatgaacac aatgaaaaaa aatattttaa ttacaggtgg tgcaggcttt ataggatctc
    13981 atgtagtaag attatttgtt accaaatatc ctgcatacaa tatcatcaat ctcgatgcac
    14041 taacctatgc aggaaattta gaaaacctga aagatataga ccaacacaaa aattattttt
    14101 ttgaaaaagt agatatttta gatgcaaaaa atttgaaaaa tatttttcaa aaacatcagg
    14161 ttacagatgt aattcattta gcagcagaat cgcatgttga ccgttctatt ttatctccat
    14221 tagattttgt ttataccaat gttattggca ctgtaaattt attaaataca gccaaagatg
    14281 cttggaagga agattttacc aaacacctgt tttatcatgt aagcacagat gaagtttatg
    14341 gtgctttagg ggctgaaggt ttttttactg aaacaacttc ttatcatcct aattcacctt
    14401 atagtgcaag taaggcatca tcagaccatt ttgtaagagc ttatgccgaa acctatcact
    14461 tacaaactat tatttccaat tgtagcaata attatggacc caatcatttt ccagaaaaat
    14521 taattccatt attcatcaat aatatcatca accaaaaacc attacctgtg tatggcgatg
    14581 gtttatatac aagagattgg ttgtttgtaa aagatcatgc aacagctatt gatatggttt
    14641 ttcatcatgg aaaaataaat gacacttata atattggtgg atttaatgaa tggaaaaata
    14701 ttgatttggt aaaaatattg tgtaagttaa tggacgaaaa attaaaaaga aatgcaggag
    14761 agagtgaaca attaataact tatgtaaaag acagacctgg acatgacaga cgttatgcca
    14821 ttgatgccac aaaaataaat caagagttag gctggaagcc aagtgtaaca ttcgaagaag
    14881 gcttatcctt aaccatagac tggtatttgc aaaatacaga atggctaaac aatgttacaa
    14941 atggtaccta tcaaagctat tacaatgaaa tgtatgccta cagaggttaa aaattgataa
    15001 aatgaaaata gaaaccacgc ctttaaaaga ttgttttatt attcagcttt cgttggtgta
    15061 ttctttatag ctcttagtgt attaggcaga gaacttgcaa tattacagta cataactgag
    15121 gtgaggtata aaaatatcta ccttttaaac agttcctata actgtttggt atcctcttaa
    15181 tcttaatact taaactgttt tttatgagaa aaatattatt tacgataacc tttttaatta
    15241 tcactcaaaa tgtattctca caaaatattt tttcatttct ttttaaagga gaaaaagaaa
    15301 tatcaatatc aactcctctt ttatggtcgc aatatgaagt aaccaacaca tttggaatat
    15361 atggagcaga taaaaaaagc aatggagaaa gttggagtat gggattaaac gcaacatata
    15421 gcaagccaat atataaaaac ttttactt
//
LOCUS       DAOQHP010000009        14838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_1927_length_14838_cov_4.051005, whole genome shotgun sequence.
ACCESSION   DAOQHP010000009 DAOQHP010000000
VERSION     DAOQHP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_14838_cov_4.051005"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLY81_00695"
     CDS             complement(<1..248)
                     /locus_tag="PLY81_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNF28777.1"
                     /translation="MNIFNNHTKLYTTAAIMYFVLTFFTAIKPALDNQQNNGPLPGTK
                     PLSGDALAGKMVYIAEGCVACHTQQVRNVDMDKMFGTRP"
     gene            complement(257..2359)
                     /locus_tag="PLY81_00700"
     CDS             complement(257..2359)
                     /locus_tag="PLY81_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF28778.1"
                     /translation="MNVKNKLKASILAVILLLVNKFATAQEHPNFINLDGKDIRSSVL
                     LFIIGCICIIIAIAYMQFKHAKEIGKNISALNKKNVLKDLNIEQLKTYINKSKSVGII
                     ILLLFSSLQSFAQTTTDKTKPLMSQPGIIITVLLIAIPILLAAWLIIARATKTVKKFN
                     NIKRLKQAEILASELREEANEESNKALKDRKTALEYSLQNIELGGSENATDDKGLILN
                     VGNTQEVHFTAVKRKAAPRPALEPAITKLVVWYLGCAAFWLVIGTGVGEYVGIKFIAP
                     DVDQVSWLSFGRLRPVHTNMVFWGWASLGMMGLGYYVVPMVSNTKLYSVKWGWIALVL
                     TNLQVTIGSISLMAGINNGGGEFREYIWPIMALLAVALILTVINFIKTIANRTTKEIY
                     ISNWYIVSSLMFTIIIALTAYLPFWQNGLGETIVQGYYMHTGVGMWFTLFTLGLLYYF
                     IPQQLNKPIYAYSLGILAFWTQILFYTIIGTHHFIFSSIPWWLQTVAIVGSVGMIIPV
                     FAATINYVMTFKGQWGKVSNSYTLPFFLVAVVYYFIVSMQGTAEAFRFTNLLWHFTDF
                     TVAHSHLGFYGIISFAIWGGAYAIIPRLTRKEPPQHLVGAHFWLAFIGLILYCFPLMF
                     GATLKGMMWMEGKPFIDSVVKMFDYWVWRAIGGSLMFFSHILFFYNFYKMSSKTIEDV
                     NVVDEAINILEQEPSTAK"
     gene            complement(2386..2820)
                     /locus_tag="PLY81_00705"
     CDS             complement(2386..2820)
                     /locus_tag="PLY81_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF28779.1"
                     /translation="MFSKSCEYAIKALIFLGQESNENKKVCVKTISSAIDAPQHFVAK
                     ILQDLTKKKLLKSTKGPNGGFYVDEENSKNSLADIIKAIDGTKIYSQCELGLKECSEK
                     NPCPVHHEYKNIKLNLIKMIENNTISTFNTQSNLGKYFLKND"
     gene            complement(2928..3671)
                     /locus_tag="PLY81_00710"
     CDS             complement(2928..3671)
                     /locus_tag="PLY81_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF28780.1"
                     /translation="MNYACTIVPVAPLRSEALHSSEMVSQLLFGECVTILDSTKDFFK
                     VSSMYDDYIGWCSKKQLTTVNENFSIQNHKQCTNIPITTALYKESNLYLSSGSFIGAL
                     KNNILTINDKIFNIHFNSTETNIQKVIHSYLNTPYLWGGKSIFGIDCSGFTQQVYKIL
                     GTKIYRDAYEQAQQGKVIGFLQEAAIGDLAFFDNENGKITHVGILLNDHEIIHASGIV
                     RIDLIDNEGIINSETKERTHHLRLIKRIF"
     gene            complement(3794..4630)
                     /locus_tag="PLY81_00715"
     CDS             complement(3794..4630)
                     /locus_tag="PLY81_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF28781.1"
                     /translation="MEKNNILTADILDIIFEGRNKAYGAYDLRKTYTKRITFAFVATV
                     TLCALFFVGSLLANSGEKKVAKILVQDVELESLKEPEEKKPEPPPPPPPPKQEPPKVE
                     MKQFTPPKIVKAEEVKPEEIPPEVTKLEEAAIGTKNQEGMKDVGVVAPVVEAVGTGKV
                     EAPKEEEDYNKIFTVVQIEAEFPGGKAAWNKYLQRNLNSSLPMDNGASPGKYTVVVTF
                     TVDKEGNVSDVQADNDPGYGTKDEAIKVIKKGPKWKPAVQNGRNVAYRHKQSITFIVS
                     EE"
     gene            complement(4691..5248)
                     /locus_tag="PLY81_00720"
     CDS             complement(4691..5248)
                     /locus_tag="PLY81_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF28782.1"
                     /translation="MAEMDTSSGGSHKKGPGVKKSKKMSTRVDLTPMVDLGFLLITFF
                     IFTTTMSLPTTMNLFLPKDTDKPEEQNKIKDNSALTILLGNDNNVFYYEGMLDNANVS
                     ANFKSSNFGGGEDGIRNIILRKKANTDEKDFMVVIKPSDECTYKNVVDILDEMTINVV
                     KRYAMVDISDVEVQLIKATEAASSK"
     gene            complement(5300..5944)
                     /locus_tag="PLY81_00725"
     CDS             complement(5300..5944)
                     /locus_tag="PLY81_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF28783.1"
                     /translation="MGRAKIPRKSTNIDMTAMCDVAFLLLSFFILTTKFKPAEAVSVS
                     PPSSVSDKAAPESDIVLITIDKDGKVFLSMDNEEKKEYIANTIANEKGLKLDVQSFKK
                     AQFIGVPFNKLTSFLQLPDDQRKGDNMPGIPCKDSTNNEMIEWLRIVRSAYMGQKINL
                     LVKGDNTSKYPSFKNVITAFKKNDLLKFQIITNPEGVPVGSELYRQAQAGIKND"
     gene            complement(6010..6879)
                     /locus_tag="PLY81_00730"
     CDS             complement(6010..6879)
                     /locus_tag="PLY81_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF28784.1"
                     /translation="MADLKPTPTATKTISNVQPKKSSNVISWIAPIVCLILGYVIWRF
                     VFGSPNNFEKPDLNGWFWPDHHKLKPNTGMAKMYEGGIVVPILIGSFFIVITFVIERI
                     LTIQKATGAGNIGEFLRKVQFHLANKDVDKALAECDKQKGSVGNVMKSGLRTYKDMIS
                     NTELSTEQKVLNIQKSIEESTALELPMLEKNLVFLSTLASVATLLGLLGTVLGMIKSF
                     SALGEDSGGATAAAELSKGISEALFNTALGIGTSAVAIIMYNVFTTRIDAITFGIDES
                     GFTLTQSFASLYK"
     gene            7230..9770
                     /locus_tag="PLY81_00735"
     CDS             7230..9770
                     /locus_tag="PLY81_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNF28785.1"
                     /translation="MKKIFFVVVVFASCNIVIAQKRTNPTSKDTTPTAAKTMPPQAAK
                     PKPGPKPFKEVITDKAISKKGLFNVHKVEDKYYFEIPNSLLGKEIMCVTRYSKVPGGA
                     GVYGGEVANQQTLKFEKGPNDNIFIRTITLISTADSSQKISTAVSNSHLDAIAAAFDI
                     KALGKDSTSSIIDVSDFFKGDNQIVSIDPNSKRRFSLSMLAGDRSYVQSINTYPINTE
                     IKTVKTYISSPMPSAGASQSSAAGFPAARDAGAVTIELNTSMILLPEKPMARRTWDKR
                     VGFFPDNFVVFSDDQQRVEDKTFAVRWRLEPKPEDVEKWKAGQLVEPIKPIIYYIDPA
                     TPPNWVPYLIAGINDWQKAFEKAGFKNAIMGKEWPKNDSTMSLEDARFSVIRYFASDI
                     ENAYGPNVHDPRTGEILESHIGWYHNVMKLVHDWYMVQTAAVNPRARKMVFDDDLMGQ
                     LIRFVSSHEVGHTIGLLHNMGSSSKTPVEKLRDKAWVEANGHTASIMDYARFNYVAQP
                     EDNINEMGLFPRIGVYDEWAIKWGYGYIPGNTEEEQNINSDKLIVKALAENPRHWFGT
                     YELGNSVDPRTQSEDLSDNVMIANEYGIKNLKRIIVNLPEWTKENGKGYDNLSEMYRQ
                     LVGQFNRYVNHVMRNVGGIYETFKSTSQQGDVFEFVPKATQKEAFNFINRNVLTTPTW
                     LLDKNILNKIQNPTNSESVQNIQISALNNLLDPSRLNRMIICANRYGNTNVYLFNDLM
                     DDTKKAVWSEAATGKAVDAYRRNLQKAHVERLINLLTFKPQAMVISGRTFATGPEIAT
                     TDIVSYTRGQLKALQAELTTASAATADRLTKFHYQDLVERIKLALNPR"
     gene            complement(9848..12019)
                     /locus_tag="PLY81_00740"
     CDS             complement(9848..12019)
                     /locus_tag="PLY81_00740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNF28786.1"
                     /translation="MKKKYLLFVAAGLAICSTSALNAQKKESTKANMKKKSKVEQPST
                     NNNPLLKKSKLQYQAPEFDKIKDKDFKPAFDYGLNEQIKQIEAIANNTDKPTFENTIV
                     ALENSGQNLRRAITIFSNLNSANTNPFLQALDEEYAPIFAAHTDKIFLNEQLFQKIKR
                     LYATRANLKLDAEDVRLIEYYNQQFEIAGANLNNEQKEELKKINEQLATLQSQFNNKL
                     LAARKAGGLIIEDVKLLDGLSSNEIDAAAENAKVAGMPGKYLLALQNTTQQPLLQNLK
                     NRTVREKLFKASWLRAEKDDENDTRETLQQLARLRLKKAQIMGKKNFAEWKLQDQMAK
                     TPENAMNLLAKMAAPAVKKAKQEAEEIQKLIDKQNGGFKLEPWDWNFYAEQVRKAQYD
                     LDDNEIKPYFEVRTVLEKGVFYAAKELYGLTFKVRKDLPVYHPDVVAYEVFDKDGKSL
                     AIYYLDFYTRDNKNGGAWMSNFVEQSYSLGQKPVIVNVYNYQKPAPGKPSLISYDDVT
                     TLFHEFGHSIHGMFASQKYISISGTNTPRDFVEFPSQINEYWALEPSVLKNYALHYKT
                     GKPIPNELVKKIKNAKTFNEGYNVTEIVAAATLDMAWHTITDESEIIPANEFEKMALD
                     KFGLLVNEVPTRYHSPYFLHIWSNGYSAAYYAYLWSEMLDFNALEYFENNGGMKRENG
                     DRFRKYILSVGNSMDLNQAFRNFTGKDPEIEPLLRGRGLIK"
     gene            complement(12072..14003)
                     /locus_tag="PLY81_00745"
     CDS             complement(12072..14003)
                     /locus_tag="PLY81_00745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF28787.1"
                     /translation="MSKKFFLLITAACILQVLNAQNVFTYGKKAVSKAEFLKAFDKNP
                     TPGETDRKKALKEYLELYINYKLKVQSAYDDKLHEQPLFKNESSSFRKQLADNIINTE
                     ANIDNLIKEVFERSQKDIHVAQIFIEIKPGVDNIEAINQIEKAHKALKAGMDFEEAVK
                     KFSNDEYTIQTKGNLGFITTFTLPYQFENEIYKLKVGEFSSVYKSSFGYHIFKNLEER
                     SAAGKRKVAQILLAMPIGATKESKQYLATLADSIYHLAINGEDFEDLVEQYSNDRATA
                     SIGGVMQEFGIGEYAPNFEQPIFDLKNKGDIAKPFLTEHGWHIVKLLDIIPVSNNFSD
                     PVTVSNIKNQIERTDRMAFAKKALVKNWMNICKYKKEPFDEKDFMIYTDSALANINFT
                     GYKNIKPETVLFSFAKQKITVAEWIKFARAVKESKEKLAQLHTLVLLIEYENIRCSEY
                     YYDHLEDYYPTLKEQSKEFDEANLLFGAMDKHVWSKASEDTVGLKKYYAINKTKYVWQ
                     PGVSAIVVTAPTQALAVQVIDSFKAGLKDWKLVLNKINNNILFDSSRFENNQLPITQV
                     IENKIGFISNPEKNTNDNSYTFMYITALHDKIETKSFDEARGFVTNNYQQVLEQKWIQ
                     ALKKKYPIVINQAVWNTIK"
     gene            complement(14005..>14838)
                     /locus_tag="PLY81_00750"
     CDS             complement(14005..>14838)
                     /locus_tag="PLY81_00750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNF28788.1"
                     /translation="LYPYRFIKEHYWDDVNFYDDRILRTPFFEPKLDEYFKYYLSPEA
                     DSIIPEIKYMLLSARTGKEMFPYLLIKFTNKYVKPEYMGQDKVFLYLFNEFYTKGDTI
                     YLDAASRKMIFERAYSLMANQIGEPAAPLNLTDTLGKTHSLYEIKSNYTLVVFWDPHC
                     GHCKEQIPRIDSIYQAKWKNMGLAVYSVYIYNDQLPDWKKFITEKKLFNWIHVYQTEE
                     AKQYEAKNSIAGFRQLYDARVTPTIYLLDDKKRIIAKQLTIEQFDDILTVKHKQIENK
                     K"
BASE COUNT         4748 a         2398 c         2189 g         5503 t
ORIGIN      
        1 ggtctggttc caaacatttt atccatatct acatttctta cctgttgtgt atgacatgct
       61 acacaacctt cagcaatata aaccatttta ccagctaatg catcgccact aagaggttta
      121 gtacctggca atgggccatt gttttgttga ttgtctaaag caggttttat agcggtaaaa
      181 aaagttaata caaagtacat gattgctgct gtagtatata atttggtatg gttgttaaat
      241 atattcatct tttaaattat ttagctgttg aaggttcttg ttctaaaata ttaattgcct
      301 catctacaac atttacatct tctatagttt ttgaagacat tttataaaag ttatagaaga
      361 aaagaatatg agagaaaaac ataaggcttc cgccaattgc tctccaaacc caatagtcaa
      421 acattttaac taccgaatct ataaatggct ttccttccat ccacatcatt ccttttaaag
      481 ttgctccaaa cattaacgga aaacagtata atattaaacc tataaatgct agccaaaaat
      541 gggcacctac taaatgttgt ggtggctctt ttcttgttaa acgtggtata attgcataag
      601 cacctcccca aatagcaaaa gaaataattc cataaaaacc aagatgtgag tgtgctacag
      661 taaagtcagt aaaatgccaa agcaaattgg taaatctaaa agcctctgca gtgccttgca
      721 tagaaactat aaagtaataa actactgcta ctaaaaaaaa tggtaaggta taactattag
      781 atactttacc ccattggcct ttaaatgtca ttacatagtt gattgttgct gcaaaaacag
      841 gaattatcat tcctacacta ccaacaatag ctactgtttg taaccaccaa ggaatagaac
      901 taaaaatgaa atggtgtgta ccaataattg tataaaataa aatttgtgtc caaaatgcta
      961 agatgcctaa gctataagca tatattggct tatttaattg ttgtggtata aagtaatata
     1021 ataagcctag tgtaaacaaa gtaaaccaca taccaacgcc tgtatgcata taatatcctt
     1081 gtacaatggt ttctcctaaa ccattttgcc aaaagggtaa ataagcagtt aatgcaatga
     1141 taatggtaaa cattaaggaa gaaacaatat accagttaga tatataaatt tctttagtag
     1201 ttctattagc aatggttttt ataaaattaa ttacagtaag tattaaagca actgcaagca
     1261 atgccattat aggccatata tattctctaa attcacctcc accattgtta atacctgcca
     1321 ttaaacttat ggagccaatg gtaacttgaa gattggttaa tacaagtgca atccaacccc
     1381 atttaacact atatagtttt gtgttgctaa ccattggtac aacataataa cctaaaccca
     1441 tcatacctaa agaagcccaa ccccaaaata ccatattggt gtgcactggc cttaaccttc
     1501 caaagcttag ccaacttact tgatcaacat cgggtgcaat aaattttata ccaacatatt
     1561 cgccaacacc tgtaccaatt actaaccaaa atgcagcaca acctaaatac catactacta
     1621 attttgtaat tgcaggttct aatgcaggcc ttggtgctgc ttttcttttt actgcagtaa
     1681 aatgtacttc ttgcgtgttt cctacattta atattaatcc tttatcatcc gttgcatttt
     1741 cacttcctcc taattcaata ttttgtaatg aatattctaa agctgttttt cggtctttaa
     1801 gggctttgtt agattcttca ttagcttctt ctcttaattc tgatgccaaa atttcggctt
     1861 gttttaatct ttttatgtta ttgaattttt taactgtttt agttgctctt gctataatta
     1921 accatgctgc caatagtatt ggtattgcta ttaatagcac agtaataata atgcctggtt
     1981 gagacattaa tggttttgtt ttgtctgtag tagtttgtgc aaaactctga agagagctaa
     2041 atagcagtag aataatgata cctacagatt tggatttgtt gatgtaggtt tttaactgtt
     2101 ctatattcaa atcttttaga acattttttt tgtttaaagc cgaaatgttt tttccaattt
     2161 ctttagcatg tttaaactgc atataagcaa ttgcaatgat tatacaaatg catccaatta
     2221 taaacaacaa tacgctactt cgtatgtctt ttccatctaa attaataaaa ttaggatgct
     2281 cttgggcggt tgcaaattta ttaaccagta ataaaattac tgctaatatg ctggctttta
     2341 atttgttttt tacattcata tttaatatcc ttttattctt ttatattaat cattttttaa
     2401 aaaatattta cctaaattac tctgtgtatt aaaagttgaa atggtattat tttcaatcat
     2461 tttaattaag tttaatttaa tgtttttgta ttcatgatgt actgggcaag ggtttttttc
     2521 tgaacactct tttaagccta actcacattg gctatatatt ttagtgccat ctatagcttt
     2581 aataatatct gctaaagaat ttttagaatt ttcttcatct acataaaaac caccattggg
     2641 gccctttgtt gattttaata actttttctt tgttaggtct tgtaatattt tggcaacaaa
     2701 atgttgtgga gcatctattg cacttgaaat tgtttttaca caaacctttt tattttcatt
     2761 agactcttgg cccaagaaaa tcaaagcttt aattgcatat tcacatgatt ttgaaaacat
     2821 ataagcagaa ttatagacaa aagtatgaca attgattcat ataaaaagag ttttttatct
     2881 gattttttta cgcaacaaaa tttttgataa aatttccata tattattcta aaaaattctt
     2941 tttattagcc ttaaatggtg ggttctttct ttagtttcag aattaataat tccttcatta
     3001 tctatcaaat ctattcttac aataccagat gcatgaataa tttcatggtc gtttaacaaa
     3061 attcctacat gtgtaatttt tccattttca ttatcaaaaa aagcaaggtc gcctatggca
     3121 gcttcttgca aaaagccaat tacctttcct tgttgtgctt gctcataagc atctctgtaa
     3181 atttttgttc ctagtatttt atatacttgc tgggtaaagc cactacaatc tataccaaaa
     3241 atacttttac caccccacaa gtatggagtg ttgaggtaag aatgaattac tttttgaata
     3301 ttagtttcgg ttgaattaaa atgaatatta aaaattttat cgttgatggt taaaatatta
     3361 ttttttaatg caccaataaa agagccagac gataaataca aattactctc cttatataaa
     3421 gcagttgtaa ttgggatatt ggtacattgc ttatggtttt gaatagaaaa attttcatta
     3481 actgttgtta gttgtttttt agaacaccag ccaatataat catcatacat ggatgaaact
     3541 ttaaaaaaat cttttgttga atctaaaatt gttacacatt ctccaaataa taattgacta
     3601 accatttcac tgctatgtaa tgcctcactc cttaatggtg caacaggtac aattgtacaa
     3661 gcataattca ttgctataaa tttatttact aaaatacagt tgaatatttt taaacatgga
     3721 aatatttttt agcaattaca aaaaaaagag gctacttaaa agcaacctct aaaattgttt
     3781 atgaaagtta taattactct tcagaaacta taaatgtgat actttgtttg tggcgataag
     3841 caacgtttct accattttgt actgctggtt tccatttagg tcctttttta attactttta
     3901 ttgcttcgtc ttttgtgcca taaccagggt cattatctgc ttgcacatcg cttacattac
     3961 cttctttatc tacagtaaaa gtaacaacaa ctgtatattt tccagggctt gcaccattat
     4021 ccattggtag gctgctattt aaattgcgtt gtaaatattt gttccaagca gctttacctc
     4081 caggaaattc agcctcaatt tgaacaactg taaatatttt attataatct tcttcttctt
     4141 taggtgcttc tactttacca gtacctactg cttcaacaac aggggcaaca acaccaacat
     4201 ctttcatacc ttcttggttt ttagtaccaa ttgcagcctc ttctagttta gtaacttctg
     4261 gtggaatttc ttctggtttt acttcttctg cttttacaat ttttggagga gtaaattgtt
     4321 tcatctcaac cttaggaggc tcttgtttag gaggaggagg tggtggtggt ggttcaggtt
     4381 ttttctcttc tggctctttt aagctttcta attcaacatc ttgaaccaaa attttagcta
     4441 ccttcttttc tcctgaattt gctaataatg aacctacaaa aaacaaggca cataatgtaa
     4501 cagttgcaac aaaggcaaaa gttatacgct ttgtgtaagt ttttcttaaa tcgtatgcac
     4561 cataagcttt atttcttcct tcgaagataa tatcaagtat gtctgctgtt aaaatgttat
     4621 tcttttccat aactaattaa ttttattata agatgcagtg ctaaataatt ttacataaga
     4681 aaagtaaaaa ttatttagat gatgcagctt cggtagcttt tattaattgt acttctacat
     4741 cagatatatc taccattgca tatcttttta caacattaat ggtcatttca tctaatatat
     4801 ctactacatt tttataagta cattcatcac ttggttttat tacaaccata aaatcttttt
     4861 catcggtatt ggcttttttg cgaagaataa tatttcttat accatcttct ccgcctccaa
     4921 aattggatga tttaaagttt gcagaaacat tagcattgtc taacattccc tcgtaataaa
     4981 acacattatt atcgttgcct aatagaatag ttaatgcact attatcttta atcttattct
     5041 gctcttctgg cttatctgta tcttttggta aaaaaagatt catagtagta ggcagactca
     5101 ttgttgtagt aaatatgaaa aaagtaatta acaaaaagcc caaatctacc atgggggtta
     5161 aatcaaccct ggtggacatt tttttgctct ttttgacgcc ggggcctttt ttatgactgc
     5221 caccggacga ggtatccatt tctgccattt cggttacttt ttatttaatt tttaaataat
     5281 ttaagagaac aaattcaagt taatcatttt taattccggc ttgtgcttgt ctgtaaagtt
     5341 cgctacccac aggcactccc tctgggtttg taatgatttg aaactttaat aaatcgtttt
     5401 tcttaaatgc tgttattaca tttttaaaag aagggtattt agaggtatta tcgcctttaa
     5461 ctaataaatt tattttttgc cccatataag cacttcttac aattctaagc cactcaatca
     5521 tctcgttatt ggtactgtct ttacaaggta ttccaggcat attatcgcct tttctttgat
     5581 catctggtag ctgtaaaaat gaagttagtt tattaaatgg tactccaata aattgggctt
     5641 ttttaaatga ttgtacatct aattttaagc ctttttcgtt ggcaattgta ttggctatgt
     5701 attctttctt ttcttcgtta tccatagata aaaaaacttt accatcttta tctattgtaa
     5761 ttaaaacaat atcagactct ggtgcagctt tatcagacac agaacttgga gggctaacag
     5821 aaactgcttc ggctggtttg aattttgttg taaggataaa gaacgataag agcaaaaaag
     5881 ctacatcgca cattgctgtc atatctatat tggtactctt cctaggaatc tttgcacgtc
     5941 ccatcgtttt ttgattttaa ggttattcac taattgttta gattcaaagt attcatcttt
     6001 ccacaaaaat tatttgtata aagatgcaaa gctttgagtt aatgtgaagc ctgactcatc
     6061 aattccaaaa gtaatagcat caattctggt ggtaaataca ttatacataa taattgcaac
     6121 agcagatgta ccaataccta acgctgtatt aaataatgct tcagaaatac cttttgataa
     6181 ttctgcagct gcagttgcac caccactatc ttctcctaag gcagagaagg atttaatcat
     6241 acctaatacc gttcctaata aacctaataa ggttgctaca gatgctaaag tggataggaa
     6301 tactaaattt ttctctagca ttggcaattc taatgctgta gattcttcaa ttgatttttg
     6361 aatatttagt accttttgtt cggttgatag ttctgtattt gaaatcatat ctttataagt
     6421 acgtaaacca gatttcatta cattaccaac actacctttt tgtttatcac attctgctaa
     6481 agctttatca acatctttat tagctaaatg aaattgcact ttacgtaaaa actcaccaat
     6541 attgcctgca ccagtagctt tttgtatagt taaaattctt tcaataacaa atgttattac
     6601 tataaagaac gaaccaatta atataggtac tactatacca ccttcataca ttttagccat
     6661 acctgtattt ggtttaagtt tatgatggtc tggccaaaac caaccattca aatctggttt
     6721 ttcgaaattg ttaggagatc caaaaacaaa cctccaaatt acatatccta aaataagaca
     6781 tacaattggg gcaatccatg aaataacatt actgcttttt ttgggttgaa catttgaaat
     6841 tgtttttgtt gctgttggag ttggttttaa gtcagccata attcaattgt tttagttttt
     6901 taaaaatact tttaaattaa taaatattta gcaattctac tgaaaaaata ttagcttttt
     6961 aatattttat ttcaagtgaa attgaaaagt tgctcaagtt aatagttttt taaattaata
     7021 attataaaca ttagaaaaat tatttgattt tttttaaatg ggaaataaaa tttaactcta
     7081 ttaattagta atactatata aattagttac tataaaatac tgttcataaa gtttatttaa
     7141 ccgctgatgt aataaaaaac aagaaattta ttaaaaaatt acccgcttcc ttaacttagc
     7201 cattctttta accaacttta taaacaatta tgaaaaaaat tttctttgtt gtagttgtgt
     7261 ttgcttcgtg caatattgtt attgctcaaa aacgaaccaa tccaacttct aaagacacaa
     7321 caccaactgc tgctaagaca atgcctccgc aagcagctaa gccaaaacct ggcccaaaac
     7381 cttttaaaga agtaattact gataaggcta ttagtaaaaa agggttattt aatgtacata
     7441 aggtagaaga taaatattat tttgaaatac ccaatagttt attaggcaaa gaaattatgt
     7501 gtgtaacccg ttacagcaaa gtacctggtg gagctggcgt ttatgggggt gaagtggcta
     7561 accaacaaac tttaaaattt gaaaaaggcc ccaacgataa tatttttatt agaacaatta
     7621 ctttaattag tactgcagat agtagccaaa aaatatctac agcggttagt aactctcatt
     7681 tagatgctat tgctgctgct tttgatatta aggctcttgg aaaagacagc actagtagta
     7741 ttattgatgt aagtgatttt tttaaaggag ataaccaaat tgtaagtatt gatccaaata
     7801 gtaaaagaag atttagctta agtatgcttg ctggagatag aagttatgta caaagcataa
     7861 atacctatcc cattaacaca gaaattaaaa ctgtaaaaac atatatttca agcccaatgc
     7921 cttcggcagg tgcaagccaa tccagtgctg ctggctttcc tgcagcaaga gatgctggtg
     7981 ctgtaactat agaattaaat acttctatga ttctattgcc agaaaaacca atggcaagaa
     8041 gaacatggga taaaagagta ggtttttttc cagataattt tgtagtattt agcgatgacc
     8101 aacaaagagt tgaagataaa acttttgcag ttcgttggag gttagaacct aaaccagaag
     8161 atgtggaaaa atggaaggct ggccaattgg tagaacctat aaagcctatt atttattaca
     8221 tagatcctgc aacgcctcct aattgggtgc cttatttaat tgctggtatt aacgattggc
     8281 aaaaagcttt tgaaaaagct ggatttaaaa acgcaataat gggtaaagaa tggcctaaaa
     8341 acgattctac catgagttta gaagatgctc gtttttctgt aattagatat tttgcatcag
     8401 atattgaaaa tgcttatggc cctaatgttc acgacccacg aactggtgaa attttagaaa
     8461 gccatattgg atggtatcac aatgtaatga aattagtaca cgattggtat atggtacaaa
     8521 ctgctgctgt aaatccacgt gcaagaaaaa tggtttttga cgatgattta atgggtcaat
     8581 taattcgttt tgtttcttct cacgaagttg gccatacaat tggtttatta cacaatatgg
     8641 gtagcagtag taaaacccct gttgaaaaat taagagacaa agcttgggta gaagctaatg
     8701 gacatacagc ttctataatg gattatgcac gttttaacta tgttgcacaa ccagaagata
     8761 atatcaatga aatgggatta tttccaagaa ttggggtgta cgatgaatgg gcaattaaat
     8821 ggggctatgg ttatatacct ggtaatacag aagaagaaca gaatattaat agcgataaat
     8881 taatagtgaa agctttagca gaaaacccac gccattggtt tggtacttat gaattgggca
     8941 actctgtaga tcctcgtaca caatcggaag atcttagtga taatgtgatg atagctaacg
     9001 aatatgggat taaaaattta aagagaatta ttgtaaactt accagagtgg actaaggaaa
     9061 acggaaaagg gtatgataat ttatctgaaa tgtatcgcca attagttggt caatttaata
     9121 gatacgtaaa tcatgtaatg agaaatgttg gtggtattta tgaaaccttt aaatcaacta
     9181 gccaacaagg agatgttttt gaatttgttc ctaaagcaac acaaaaagaa gcatttaatt
     9241 ttattaatag aaatgtatta actacaccaa cttggttatt ggataaaaat attttaaata
     9301 aaatacaaaa tccaaccaat agcgaatcgg tacaaaacat tcaaattagt gctttaaaca
     9361 atttactaga tccatcaaga ttaaacagaa tgattatttg tgctaatcgt tatggcaata
     9421 caaatgttta tttatttaac gatttaatgg atgataccaa aaaggcagtt tggagcgaag
     9481 cagcaactgg aaaagctgta gatgcttata gaagaaactt gcaaaaagcc catgtagaaa
     9541 gattaattaa tttattaacc tttaaaccac aagctatggt tatatctggc agaacatttg
     9601 ctacaggccc tgaaatagcc acaacagata ttgtttctta tactcgtggt caattaaaag
     9661 cattacaagc agaattaact actgcaagtg ctgctactgc agatagatta actaagtttc
     9721 attatcaaga tttggttgaa agaataaaac tagcattaaa ccctcgttag ttttcttgct
     9781 atagaatcta aaaaagaaga ggttgcaaat ttttgcaacc tcttcttttt ataaaaaact
     9841 attcaggtta ttttattagt cccctacctc ttaataaagg ttcaatttct gggtcttttc
     9901 ctgtaaagtt tctgaatgct tggtttaaat ccatagagtt accaacagat aaaatatatt
     9961 ttctaaacct gtctccattt tctcttttca ttccaccatt attttcaaaa tattctagag
    10021 cattaaaatc tagcatttca ctccataaat atgcatagta agctgcagag taaccgttag
    10081 accaaatatg caaaaaataa ggagaatgat aacgggtagg tacttcattt accaataagc
    10141 caaatttatc taaagccatt ttttcaaatt cgtttgctgg tataatttca ctctcatctg
    10201 taattgtatg ccaagccata tctaaagtag cagcagcaac aatttcggtt acattatatc
    10261 cttcgttaaa tgttttagca ttttttattt ttttaactaa ttcatttggt attggttttc
    10321 ctgttttata atgcaaagca tagtttttta atacagatgg ttctaatgcc caatattcat
    10381 taatttgaga aggaaactct acaaaatctc ttggggtatt ggtgccagaa atagaaatat
    10441 atttttggct tgcaaacatg ccatgaatac tatggccaaa ctcatgaaat aaagtggtta
    10501 catcatcgta agaaattaaa gatggtttac ctggtgctgg tttttgataa ttgtacacat
    10561 ttacaattac aggcttttgt cctaaactgt aagattgttc aacaaaatta ctcatccatg
    10621 ctcctccgtt tttattatct ctagtataaa aatctaaata ataaattgct aatgattttc
    10681 catctttatc aaatacttca taagccacaa catctggatg ataaacaggt aaatctttac
    10741 gtactttaaa ggttaagcca tacagttctt tagctgcata aaaaacacct ttttccagta
    10801 cagttcttac ttcaaagtaa ggtttaattt cattatcatc taaatcgtat tgtgcttttc
    10861 ttacttgttc tgcataaaaa ttccaatccc aaggttctaa tttaaagcca ccgttttgtt
    10921 tgtcaattaa tttttgaatt tcttctgctt cttgttttgc ttttttaaca gcaggggcag
    10981 ccatttttgc taataagttc attgcatttt ctggggtttt tgccatttgg tcttgtaatt
    11041 tccattctgc aaaattcttt ttacccatta tttgtgcctt ttttaaacgt agccttgcta
    11101 attgttgcaa agtttctcga gtgtcgtttt catcgtcctt ttcagctcgt aaccatgatg
    11161 ctttaaataa tttttctctt acagttctat tttttaaatt ttgtaaaagc ggttgttgcg
    11221 tggtattttg taatgctaat aaatatttac ctggcatacc tgcaactttt gcattttcgg
    11281 cagctgcatc tatttcgtta gaagagaggc cgtctaaaag tttaacatcc tcaataatta
    11341 aaccacctgc ttttctagca gccaataatt tattattaaa ttgagattgc agggtagcta
    11401 attgttcgtt tatttttttt aattcttctt tttgttcgtt gtttaaattg gctcctgcaa
    11461 tttcaaactg ttgattataa tattcaatta atcttacatc ttctgcatct aattttaggt
    11521 ttgctctggt agcatataac cgttttattt tttgaaaaag ctgctcgttt aaaaatattt
    11581 tatcggtatg tgcagcaaaa attggtgcat attcttcatc taaagcttgt aagaagggat
    11641 ttgtattagc actatttaag tttgaaaaaa tggtaattgc cctacgtaaa ttttggccac
    11701 tattttctag tgcaacaatt gtattttcaa aagttggttt atctgtattg tttgcaattg
    11761 cctctatttg ttttatttgc tcatttaagc cataatcaaa tgcaggctta aaatctttgt
    11821 ctttaatttt atcaaactct ggtgcttggt attgtagttt actttttttt aataaggggt
    11881 tgttatttgt actgggttgt tctactttac tttttttttt catgttagct tttgtacttt
    11941 cttttttttg agcatttaat gctgatgtac tacaaattgc caggcctgct gcaacaaaga
    12001 gtagatattt ttttttcatg gtcaattagt ttaaatggta aaagatatat tttatgaagt
    12061 aaaatatttt attatttaat agtattccaa acagcttgat taataactat ggggtatttt
    12121 ttctttaaag cctgaatcca tttttgttct aacacttgtt gatagttgtt ggtaacaaag
    12181 cctctagctt cgtcaaaact ttttgtttct attttatcat gaagtgcagt aatatacata
    12241 aatgtataac tgttgtcatt tgtgtttttt tctggattac taataaagcc aattttgttt
    12301 tctataactt gtgttattgg aagttggtta ttttcaaacc tgctactatc aaataaaata
    12361 ttattgttta ttttatttaa aaccaatttc caatctttta aaccagcttt aaaactgtct
    12421 ataacttgaa ctgccaaggc ttgtgttggt gctgttacaa caattgcaga aacacccggc
    12481 tgccatacat attttgtttt gtttatagca taatattttt ttaaaccaac tgtatcttca
    12541 ctggccttgc tccatacatg tttatccata gcaccaaaca gtaaattagc ttcatcaaat
    12601 tctttgcttt gttctttaag tgttggataa taatcttcta aatggtcgta ataatattcg
    12661 ctacatctaa tattttcata ttctattagc aatacaagtg tgtgtagttg tgcaagtttt
    12721 tctttacttt cttttactgc tcttgcaaat tttatccatt cagctacagt aattttttgt
    12781 ttagcaaaag aaaataaaac agtttctggt ttaatatttt tgtatccggt aaagttgata
    12841 tttgctaagg cactatctgt ataaatcata aaatcctttt cgtcaaaagg ttctttttta
    12901 tatttacaaa tgttcatcca gtttttaact aatgcttttt tagcaaaagc cattctatct
    12961 gttctttcta tttgattttt aatgtttgaa actgtaactg ggtctgaaaa attattacta
    13021 accggtatta tatctagcaa ttttacaata tgccatccat gttctgttaa gaatggttta
    13081 gcaatatctc ctttgttttt taaatcaaaa atgggttgtt caaaatttgg agcatattcg
    13141 ccaattccaa attcttgcat tacccctcca atacttgctg tagccctgtc gttgctatat
    13201 tgttctacta aatcttcaaa gtcttctcca ttaatagcta aatgataaat actatctgcc
    13261 agtgtagcta aatattgttt gctttctttt gttgcaccaa ttggcatagc taataatatt
    13321 tgagctactt ttctttttcc tgctgctgat ctttcttcta agtttttaaa aatatggtag
    13381 ccgaaactgc ttttataaac agatgaaaac tctccaactt ttaatttata aatttcattt
    13441 tcaaattgat aaggtagtgt aaaagtggta ataaaaccta ggttgccttt tgtttgaatt
    13501 gtatattcat cgttagaaaa ttttttaaca gcttcttcaa aatccattcc tgcctttaat
    13561 gctttgtgag ctttttctat ttggtttata gcttctatat tatcaacacc tggttttatt
    13621 tcaataaata tttgagctac atgaatatct ttttggctac gttcaaatac ttctttaatt
    13681 aaattatcaa tatttgcttc tgtattaata atattatctg ctaattgttt tctaaagcta
    13741 ctgctttcgt ttttaaacaa gggttgttca tgtaatttat catcgtaagc agactgaact
    13801 tttaatttat aattaatgta aagttccaaa tattctttca atgctttttt tctgtccgtt
    13861 tctcctggtg tagggttttt atcgaatgct tttaaaaact ctgccttact aactgctttt
    13921 tttccataag taaatacgtt ttgtgcatta agtacctgaa gaatgcaagc tgctgtaatt
    13981 aataaaaaaa atttcttact catcttattt tttattttct atttgtttgt gtttaacagt
    14041 taatatatca tcaaattgct caatggttaa ttgcttggca ataattcttt ttttatcatc
    14101 aagtaaatat attgttggtg ttactctggc atcatatagc tgcctaaagc ctgcaatact
    14161 gtttttggct tcatattgtt tggcttcttc tgtttgataa acgtgaatcc agttaaataa
    14221 ttttttttct gtaataaact ttttccagtc gggcaattgg tcgttataaa tatatacaga
    14281 atatacagcc aatcccatat ttttccattt tgcttgatag atactatcaa ttcgtggaat
    14341 ttgttcttta caatgcccac aatgaggatc ccaaaaaact actaaagtgt aatttgattt
    14401 tatttcgtac aaagaatggg ttttgcctaa tgtatctgtt aaatttaaag gtgctgcagg
    14461 ttctccaatt tgatttgcca ttaaactata agctctttca aaaatcattt ttcttgatgc
    14521 agcatctaaa taaatggtat cgccttttgt ataaaattcg ttaaacaaat aaagaaaaac
    14581 cttatcctgc cccatgtact ctggttttac atatttattt gtgaacttaa ttaataaata
    14641 tggaaacatt tctttaccag ttcttgcaga aagtaacatg tatttaattt caggaatgat
    14701 agaatctgcc tctgggctta ggtagtactt aaaatattca tctaattttg gctcaaaaaa
    14761 aggagttctt aaaattctat catcataaaa atttacatca tcccaataat gttctttaat
    14821 gaaacgataa gggtataa
//
LOCUS       DAOQHP010000010        14128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2065_length_14128_cov_4.529027, whole genome shotgun sequence.
ACCESSION   DAOQHP010000010 DAOQHP010000000
VERSION     DAOQHP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_14128_cov_4.529027"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(744..2846)
                     /locus_tag="PLY81_00755"
     CDS             complement(744..2846)
                     /locus_tag="PLY81_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNF28789.1"
                     /translation="MSIIQNIREKGAWLTFGFIAVALIAFILMDGVGRGGAGMTATST
                     IGKVNGTAIELGDFDQKLNMYSQGGQDRNSILPQLWNMEVERILLEQECEKLGITVTG
                     KEVGEILFGENSPLKREKQFVDDNGNFKAEEARQAFAQLKNSKNNDNLQGVIQTYVEP
                     IKQQALKAKYDAILQQSVYVPTWLVEKTKADNSGLAKINYVFVPYTSISDSTVKDISD
                     NEIAAYIKKHPAGFEQKEEGRSFSYVVFSTAPSAIDSLNTLNNILSLKPEFAKEENAE
                     AYLGRIGTEIPYLNAFLPKKDMKMPNADSIKALADGSTFGPYLDGGNLVIAKMIGKRN
                     LPDSVKVRHILVKTEDRRNPVLEDSIAKKRIDSVEALVKSGADFNQLVQKYSDDGGSK
                     ATKGEYDFALQQFSGISKEFAETIFYGKAGDKKIVKVENDAYSGYHYIEVISQKNFSD
                     AVKVAYLAKPILASNETMNAANSAALQFAAKIKNKKQFEEEALKLNKSVMIAQDVKEN
                     DFTIPNFQQASVRNLVRWLYEKKVDAVSEPMEIGENFVVSILTGVNKVGLKSVASARP
                     MVEPFVRNEKKAKLIIESKFKGSTLEEYASSSGTSIQSSDSVYFSNPVIANMGYDLKV
                     TGSSFNKDMVGKVTPPMASNAGVVAVKVITIGTIPPVQDDAAIKQNILGQMRNAAFRS
                     AQALRNAATIKDYRFKVY"
     gene            3402..4409
                     /gene="nadA"
                     /locus_tag="PLY81_00760"
     CDS             3402..4409
                     /gene="nadA"
                     /locus_tag="PLY81_00760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNF28790.1"
                     /translation="MNINIAKEQVAEKGFLDIEVSPTLDLFAEIEKLKKEKNIVLLAH
                     YYQEPDIQDVADYIGDSLGLAQQAAQTNADIIVFAGVHFMAETAKILSPTKKVLLPDL
                     NAGCSLADSAPPKLFKSFKDKHPDHIVVTYVNCSAEIKALSDIICTSSNAEKIIESIP
                     KEQPIIFAPDRNLGAFLNKKTGRNMVLWNGSCIVHEIFSLEKLTKLKIRHPKAKFIAH
                     PECEEAVLNVADFIGSTSQLLSYTEKNEATEFIVATEAGILYQMQQNSPQKTFIPAPP
                     NNSCACNDCPYMKLNTLEKLYLCMQYETPEITIDEKLRLQALKSLDRMMEISKAAGLI
                     K"
     gene            complement(4414..5079)
                     /locus_tag="PLY81_00765"
     CDS             complement(4414..5079)
                     /locus_tag="PLY81_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF28791.1"
                     /translation="MAQICKIISLELLTQDVLKLIVEKPENLHFNPGQAVDVSINKPN
                     WKDKIRAFTFTSLPTDNFVEFIIKTYPQHNGVTNELLFLSVGDELIIGDVFGDISYKG
                     NGVFIAGGAGITPFLSILKHLQQQNKIANNILLFANKTKADIILEPFFKNLLKENFVN
                     ILSDEKLDNYEHGFINKTMLEKYTASNNIFYLCGPPPMMQAVEGFLIKLNINPENIIK
                     ESF"
     gene            complement(5086..5730)
                     /locus_tag="PLY81_00770"
     CDS             complement(5086..5730)
                     /locus_tag="PLY81_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF28792.1"
                     /translation="MEELIKLSDIEIIDSILNGNKHLFEIIVRRYNPYLYKIGRSYNY
                     THEDTQDLMQESFIDAYKNLQQFSGKSSFKSWLIKIMLHNCFHKKGKLNFKKSSMKEI
                     NENDKPIFNMETKKLNYENKELSHILENALTNLPLHYRMVFSLREINGFNINETADIL
                     NISETNVKVRLNRAKSMLRSQVEKVYSKAELFEFNLIYCNAIVNRVMSKINNLK"
     gene            5859..6806
                     /locus_tag="PLY81_00775"
     CDS             5859..6806
                     /locus_tag="PLY81_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNF28793.1"
                     /translation="MKNPVICIGAALVDELYSATRPMLMSTTNDAFVHRSAGGVARNI
                     AHQLALLNVPVQLISVFGNDGEGDWLKTICSYVNIKLDAAITNQALTGKYTGILNYDR
                     SLFTALLTNTSLDAITPQHLQQHESLLSTAFCIVADANIHPDTVAWLAFFCFKKNIPF
                     IIEPVSVPPAQKFAKMELKGIQMLTPNEDELPAMCSGGGTTFEENAIELLNRGVEKVW
                     LHRGKRGSMLFTKENTYSLEAPPVDDADIKDVTGAGDGSLSGWLMGKYLGKDDEDCLK
                     ISHTLSAEILQVNGAIATHLDQEKLLSLVTKYYQQRNFL"
     gene            6869..9721
                     /gene="polA"
                     /locus_tag="PLY81_00780"
     CDS             6869..9721
                     /gene="polA"
                     /locus_tag="PLY81_00780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNF28794.1"
                     /translation="MTEKKLFLLDAYALIFRAYYALIRNPRITSNGKNTNAQFGFTNT
                     LVELINKQKPTHIAVCFDVGGSVIRETEYADYKANRQETPEDLLAAVPDIKKIIEGFN
                     IPVIGIEGYEADDVIGTLAWEAHDAGFQVFMVTPDKDYGQLVREGISIYKPATQGNDV
                     EILGPKQVCEKWDIERVDQVIDILGLMGDAVDNIPGIKGVGEKTAAKLLKEYDNLENV
                     LANAENIKGALGEKVKAGKADAILSKKLATIITNVPVSFNEEEFKLKEWNKEALNEIF
                     AELEFRTLGKRLLGEDFTIASNNVQTDLFGNIVESKTPKSEKKQKETTKEQEEFNLIN
                     TTVDEENKAFKNINNTTHNYILIDSEEKIDNLIKQLLQQTEICFDTETTNIDANNAEL
                     VGISFSYKPLEAFYIPLSQNFTQAQQILEKFIPLFNNTNIVWIGQNIKYDLLVLKWYN
                     IQIQGNVFDTMLAHYVVEPEGKRSMDILSEQFLQYQPIPIEDLIGKKGKTQGNMRDIE
                     VEKIKEYAAEDADITLQLKQKIEPLLKEKEVEKVFYEVENPLVKVLTAMEFEGIKIDE
                     AFLKEYSKELSKEAKAAEEAVYKKSEVRFNLASPKQLGEVLFDKLKLDSSAKKTKTGQ
                     YQTGEDILQKLSAKGHSIAEDILVYRELTKLKSTYVDALPEIINSKTGRVHTTYGQAV
                     AVTGRLASNNPNLQNIPIRTAKGREIRKAFIPRDTNRVLISADYSQIELRIVAAISND
                     ENMCAAFRAGKDIHTATAAKVYNVAENEVTKEMRYKAKSVNFGIIYGQGAFGLADNLG
                     ISRTEAKEIIDNYKREFPGIQKYMDDTINFARENGFVQTIMGRKRWLKDINSSNFTVR
                     GFAERNAINSPIQGTAADMIKMAMIKIHTALQKEQLQSKMILQVHDELVFDVLSSEIE
                     LIKPLILNNMKSAMVLPNQVPVEAELGIGNNWLEAH"
     gene            9867..10583
                     /locus_tag="PLY81_00785"
     CDS             9867..10583
                     /locus_tag="PLY81_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28795.1"
                     /translation="MKLSFSLLLVLATKTLMAQAQNTQELNNQSASNNLQTIYHKTVQ
                     YKQNVFTGIEYFRNYGKINGHAFWNENEFINGNVGYDGNIYPNLPLKLDLMVEELITH
                     GVNQGVQIKIIREKIDSFTIGSVRFLHLTPDKNKVPDGFYEILHSQKYTVFCKRNKLI
                     KEVIVQNELERNIEEEAVYFLKNQDNIIVIKNRQILFDLFRDKKNQVLDYMSNLNLNF
                     RKQKEAFIVESIKYYEQITN"
     gene            10588..13353
                     /locus_tag="PLY81_00790"
     CDS             10588..13353
                     /locus_tag="PLY81_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF28796.1"
                     /translation="MKFSLRLLTLLALIILFSYAKAQNENNITLDLKRASFAEFVQNV
                     EKQTNYFFYYNKVDTDSVFITISVQNATIATVLNTVFENTELYFSIADNKKIFVSRNV
                     QLLTNLGTLFFKTNEQVNKKEKTITVIEDSIEETNTNDVAIQNKLIVIGKPNAAVSGK
                     VLITGQVKNGKNNEPIVGAVIAAGKTNVVTDYLGKFTISLNKGRNMLTASSIGMRDAV
                     RQLLVQSSGNINIEMQENITALKGVVVTATKRNTGVKTPKMGVEKITIKQLKQQPVLF
                     GEADVLRAVLTLPGVTSVGEASTGFNVRGGSVDQNLILFNDATIFNPSHLFGFFSAFN
                     PSVVKGVELYKGSIPEKFGGRISSVLDVSVQEGNKKKFGGAGGIGPLTSHLMFEGPIK
                     KDKGSFIIGFRTTYSDWILKNLKDVSFKNSKAAFNDLSIHLSQQINTKNFLYITGYTS
                     KDNFRLDKDTSYAYKNNNINIKWKHIFKNNLISNVTAGYDNYNYGIESSLKPLSAFTL
                     KFNINQYYLKADFTKSYGNVHRINFGYNGNFMKLNPGSYAPSGKVSLVNAINIQQEKG
                     IENAIYLGDKMELSSKLAVEAGIRVSAFTAIGSSKVYKYYNGLERDDANIQDTIFYKN
                     GQPIKTYWGPEVRLSTRYTINETSSIKAGFSTQRQYIHMLSNTTAISPTDIWKLSDTY
                     IKPQIGQQVSLGFYKSFPKSDVEVSVEVYYKWLQNFLDYKSGALLLLNQHIETDVLNS
                     KGKAYGVEFMIKKNSGKLNGWLSYTYSRTMLQTDEPTTSYKVNKGSYYPANFDKPHSI
                     NFVGNYKFSHRFNVSLTTVYSTGRPITLPIAIYNYGGGDRVYYSDRNQYRIPDFFRAD
                     FSMNIEGNHKIKKLAHSSWSLGIYNITSRKNPYSVYFATENGKIKGYQLSIFGSAIPF
                     ITYNFKF"
     gene            13370..>14128
                     /locus_tag="PLY81_00795"
     CDS             13370..>14128
                     /locus_tag="PLY81_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNF28797.1"
                     /translation="MKRHIIYLVTIVAIITGCRENYTPPEVTTDHKILVVEGFIDVNK
                     DSSYFKLSRTQKLGSVSSVKPEAGANISILSSNGTVLTQFKERANGQYVVYGATLLPT
                     EFYRLKITTSGAVVYQSDLIKGKITPAIDSVTWKIDNDKNGIQFYVNSHDAANETKYY
                     KWDCEEVWEYRAYYESQYKYLGDTIIEPRLPGEQIYRCWRTVLPTQIFINTTEKLSSD
                     VISNEKILFVGKGSNRFDQRYSILVKQRALTKEAF"
BASE COUNT         5354 a         2047 c         2188 g         4539 t
ORIGIN      
        1 aaagctgctt atgaaatttc tttttgtcat tcttttgata aaataattgt aaatgataat
       61 ttagaaactg cttgtaaaga agctgaaaag attatagaaa cttttttatg tcaatcctga
      121 gcctcctttt aattcatctt atcctttttt gtcatcctaa gcgtagcgaa ggatctgctg
      181 aataacagca acaaagaaca gagagattcc tcgtaaactc ggattgacaa aatgcataga
      241 ttcctcgcag gctcggaatg acaaatctta ttttgtttca cttcgcttac agcatgtcat
      301 cgttttgcct tttttgtcat cctgagcgta gcgaaggatc tccagtagca caatggttca
      361 aacagatgtt tcacttacgt tcaacatgac aacaaaaaca gatagattcc tcctaaactc
      421 ggaatgacaa tgcttttttg ttttattttc attgactaca tttcatactg agccccattt
      481 tatttcatct tatccttttt ttgtcatcct gagcgaagcg aaggatctac tgaaaagcag
      541 caccaaagaa caaagagatt cctcgcaagc tcggaatgac aaagcatatt tagtttcatt
      601 ttatttgtat tgacaggctt ttaaagaata attcaataaa aactcagtag tagattaatt
      661 ttaacttcgg taataaaggg gatattccaa agaataaaaa aaaggagcta aatattagct
      721 cccttatata aaatattcta taattaatat actttaaacc tataatcttt tatagtagct
      781 gcattgcgta aagcttgggc acttctaaaa gcagcattac gcatttgtcc taaaatattt
      841 tgtttaatag ctgcatcatc ttgtactggc ggtatagtac caattgtaat aacttttaca
      901 gcaaccacac ctgcattact tgccattggt ggtgttactt taccaaccat atctttatta
      961 aatgaagagc cagtaacttt taaatcgtac cccatgtttg caattacagg gttagaaaaa
     1021 taaacactat ctgatgattg aatagatgta ccagatgagc ttgcatactc ttctaaggtt
     1081 gagcccttaa atttagactc aataattagt tttgcttttt tctcgtttcg tacaaatggt
     1141 tctaccattg gccttgcaga tgctacagat tttaagccta ctttattaac tcctgttaaa
     1201 atagatacta caaagttttc gccaatttcc attggttcac ttactgcatc tactttcttt
     1261 tcatataacc atctaactaa atttctaaca gatgcctgtt gaaaattagg aattgtaaaa
     1321 tcgttttctt ttacatcctg agcaatcata actgatttat ttagctttaa tgcttcttct
     1381 tcaaactgtt ttttattttt aatttttgca gcaaattgta atgcagcaga attggctgca
     1441 ttcatggttt cgttgcttgc taagattggt tttgctaaat atgcaacttt aactgcatcg
     1501 ctaaaattct tttggcttat cacctctata taatggtagc cagagtatgc atcattttct
     1561 acttttacta tttttttatc gccagcctta ccataaaata ttgtttctgc aaactctttg
     1621 cttataccag aaaattgttg taaagcaaaa tcgtattctc cttttgttgc tttacttcca
     1681 ccatcatcgc tatatttttg aactaactga ttaaaatctg ctcctgactt tactaaagct
     1741 tctacactat caattctttt tttagcaata ctatcttcta aaacggggtt tcttctatct
     1801 tctgttttaa ctaaaatatg tctaactttt acactatctg gtaaatttct tttgcctatc
     1861 attttagcaa ttaccaagtt tcctccatct aaatatggcc caaacgtact accatctgct
     1921 aacgctttta tactatcagc atttggcatc ttcatatctt ttttaggtaa aaaggcattt
     1981 aaataaggaa tttctgtacc aattcttcct aaataagctt ctgcattctc ttcttttgca
     2041 aattctggtt ttaagcttaa tatattattt aatgtgttta aagaatctat agcagatggg
     2101 gctgtactaa atactacata agaaaaactt cttccttctt ctttttgttc aaaccctgct
     2161 gggtgttttt taatataagc tgcaatttca ttatctgaaa tatctttaac tgtactatca
     2221 gaaatactag tatatggtac aaaaacataa ttaatttttg ctaaaccgct gttatctgcc
     2281 tttgtttttt ctactaacca tgttggcaca tatacacttt gttgtaatat agcatcgtac
     2341 tttgctttta aagcttgttg tttaattggc tctacatagg tttgaataac gccttgcaaa
     2401 ttatcattat tcttagagtt ttttaattga gcaaatgcct ggcgtgcttc ttctgcttta
     2461 aagtttccat tatcatctac aaattgtttt tctcttttta atggtgagtt ttctccaaat
     2521 aaaatttctc caacttcttt accagtaacg gttataccta atttttcaca ttcttgttct
     2581 aacaaaattc tttctacttc catattccat aattgaggta aaatagagtt tctatcttgc
     2641 ccaccttgtg aatacatatt taacttctga tcaaaatcac ctaactcaat agcagttcca
     2701 ttaaccttac ctattgttga tgttgctgtc ataccagctc cacctcttcc cacaccatcc
     2761 attaatataa atgcaataag agctactgca ataaagccaa aagttaacca agcacctttt
     2821 tctctgatat tttgaataat agacataatt agtatataaa attttaagga acgcaaaaat
     2881 aggggaatat acttattgaa caaattgtgg ataaacaatc aaaaccttta tttggcttga
     2941 tttataaatt ttaaataaaa tagcttttag tttagataaa aattgagaag aaaatgctta
     3001 aaatatttat taagataagt ttgttggtaa attgaatttg gtgtgaatat ttaataaaaa
     3061 aaattaaaaa cactgttgaa agaattagaa taaatacaag aaagttttaa gattatagga
     3121 aaacatgttt tgttttaact ttttactcta tcttatttaa cagttaataa atggtttatt
     3181 tgagattgtg aataatagaa taatttttat taagtactgt ataaaaaata caaaaaggct
     3241 tagcactatt atattttaca ttgttaatat aaggttagta atagtgtata accaatctat
     3301 tagttaactt tgctacttgt taataaaaaa cttatcccaa tattaacagc tataatagta
     3361 atagttgttt atataaatat atactattaa atattattac aatgaatatt aacatagcaa
     3421 aagaacaagt tgccgaaaaa ggatttttag atattgaagt ttcgccaact ttagatttat
     3481 ttgcagagat agaaaaatta aaaaaagaaa aaaatatagt tttattagca cactattatc
     3541 aagaaccaga tatacaagat gttgcagatt atattggcga tagtttaggg cttgcacaac
     3601 aggcagcaca aactaatgca gatattattg tttttgctgg tgtacatttt atggcagaaa
     3661 cagcaaaaat tttaagccca actaaaaaag ttttattacc cgatttaaat gcaggctgtt
     3721 ctttagcaga tagtgcacca ccaaaattat ttaaaagttt taaagacaaa cacccagacc
     3781 atatagtagt aacttatgta aattgtagtg ccgaaataaa agctttaagt gatataattt
     3841 gtacctcgag taatgcagaa aaaattatag aaagtattcc aaaagagcag ccaataattt
     3901 ttgcaccaga tagaaattta ggtgcttttt taaacaaaaa gaccggcaga aatatggttt
     3961 tatggaatgg gtcttgtata gtacacgaaa tatttagctt agaaaaatta accaaactta
     4021 aaattcgtca tccaaaagcc aaatttatag cccatccaga atgtgaagaa gcagttttaa
     4081 atgttgctga ttttattggt agtacatctc aattattaag ctataccgaa aaaaatgaag
     4141 caacagaatt tattgttgca acagaagctg gaattttata tcaaatgcaa caaaatagcc
     4201 cacaaaaaac atttatacca gcaccaccaa ataattcttg tgcttgtaac gattgtcctt
     4261 atatgaagct aaatacatta gaaaagttgt atttatgtat gcaatatgaa accccagaaa
     4321 taactattga tgaaaaatta aggttacaag cattaaaatc tttagataga atgatggaaa
     4381 ttagtaaagc agcaggttta ataaaataat taattaaaaa gattctttaa ttatattttc
     4441 tggattaata tttagtttaa ttaaaaaacc ttctacagct tgcatcattg gcggagggcc
     4501 acacaaataa aaaatattat ttgaagcagt atatttttct aacatggttt tattaataaa
     4561 accatgttca taattatcta atttttcatc ggataaaata ttgacaaagt tttcttttaa
     4621 taaattttta aagaaaggtt ctaaaataat atctgccttt gttttatttg caaacaataa
     4681 aatattatta gcaattttat tttgttgttg taagtgtttt aaaatactca aaaaaggagt
     4741 aataccagca ccaccagcaa taaaaacacc attgccttta taagaaatat cgccaaaaac
     4801 atcaccaata attaattcat cgcctacact taaaaataaa agttcgtttg taacaccatt
     4861 atgttgtggg tatgttttta taataaattc aacaaaatta tctgttggta aagacgtaaa
     4921 agtaaaagct ctaattttat ctttccaatt tggtttattt atagaaacat caacagcctg
     4981 acctgggtta aaatgtaaat tttcaggttt ttcaactata agtttcagaa catcctgtgt
     5041 gaggagttct aaagaaataa ttttacaaat ttgtgccatg ctttgttatt ttaaattgtt
     5101 tattttactc attactctat tcacaatagc attacaataa attaaattaa attcaaaaag
     5161 ttcggcttta ctgtaaacct tttctacctg cgaccttaac atagattttg ccctatttag
     5221 cctcactttt acatttgttt cagaaatatt taaaatatcg gcagtttcgt taatattaaa
     5281 accatttatt tctcttaaag aaaaaaccat tctataatgt agtggtaaat tagttaatgc
     5341 attttctaga atatgactta actctttatt ttcataattt aattttttag tttccatatt
     5401 aaaaatgggt ttatcgtttt cattaatttc tttcatacta ctttttttaa aatttaattt
     5461 tcctttttta tgaaaacaat tatggagcat aattttaatt aaccaagact taaagctaga
     5521 cttgccagaa aactgttgta agtttttata agcatctata aaactttctt gcattaaatc
     5581 ttgagtatct tcatgggtat aattataact tctaccaatt ttatacaaat aagggttata
     5641 acgcctaaca attatttcga ataaatgttt attgccattt aagatgctat caataatttc
     5701 aatatctgat aatttaatta attcttccat tttaataaat actaaataag tagagtaatc
     5761 atctaaccaa aaagttacaa aaaagaattg aaaaaggtaa ttttttttta tttcataaaa
     5821 ataaaaaaca gccatcacaa aacagtacat ttgcagctat gaaaaatcct gtaatttgta
     5881 ttggtgcagc attggtagat gagttgtaca gtgccacaag acccatgtta atgtcaacca
     5941 ccaacgatgc ttttgtacat agaagtgcag gaggtgtagc acgtaatatt gcccatcaat
     6001 tagcattatt aaatgtacct gtacagctta taagtgtatt tggaaatgat ggagagggag
     6061 attggcttaa aacaatttgt agctatgtta atattaaatt agatgcagcc ataaccaatc
     6121 aggcattaac aggtaaatac acaggtattt taaattacga tcgttctctt tttactgcat
     6181 tattaaccaa cacatcatta gatgctataa caccacagca tttacaacag cacgaaagtt
     6241 tattatctac agcattttgt atagttgcag atgccaatat tcatccagat actgtggcat
     6301 ggttagcttt tttctgtttt aaaaaaaata taccatttat aattgaacct gtttcggtac
     6361 cacctgcaca aaagtttgct aaaatggaat taaaaggtat tcaaatgcta acacctaatg
     6421 aagatgaatt accagctatg tgtagtggcg gcggaacaac ttttgaagaa aatgctatag
     6481 aacttttaaa cagaggggta gaaaaagttt ggctacatag aggaaaaaga ggctctatgc
     6541 tttttacaaa agaaaatact tattctttag aagcaccacc tgtagatgat gcagatataa
     6601 aagatgtaac aggagcaggt gatggcagtt taagtggttg gctaatgggt aaatatttag
     6661 gaaaagatga tgaagattgt ttaaaaatat cgcacacatt aagtgccgaa attttacaag
     6721 taaatggtgc cattgcaaca catcttgacc aggaaaaact actttcttta gtaaccaaat
     6781 attatcagca aagaaatttt ttatagctat ataaaagaaa aaaatagata aacatttggg
     6841 tgcaaaaaat aatgtatatt tttacccaat gaccgaaaaa aagctatttt tattagatgc
     6901 atatgcatta atttttagag catactatgc cttaattcgt aatccacgta ttacaagcaa
     6961 cggaaaaaat accaatgccc aatttggttt taccaatact ttagttgaat taataaataa
     7021 gcaaaaacca acacatattg ctgtttgttt tgatgttggt ggttcggtaa taagagaaac
     7081 agaatatgcc gactataagg caaacaggca agaaacgcca gaagatttat tggcagccgt
     7141 accagatata aaaaagataa ttgaagggtt taatatacct gtaataggca tagaaggata
     7201 cgaagcagat gatgtaattg gtaccttggc atgggaggct cacgacgcag gcttccaagt
     7261 ttttatggtt acgccagata aagattatgg gcaattggta agagaaggaa taagtattta
     7321 taaaccagcc acacaaggta atgatgtaga aattttagga ccaaaacaag tatgtgaaaa
     7381 atgggatata gaaagagtag atcaggtaat agatatttta ggattaatgg gagatgcagt
     7441 agataatatt ccaggaatta aaggtgttgg agaaaaaact gcagcaaagc ttttaaaaga
     7501 atatgacaat ttagaaaacg ttttagccaa tgctgaaaat attaaaggtg cattaggaga
     7561 aaaagtaaaa gcaggtaaag cagatgctat tttaagtaaa aaattagcaa ccataattac
     7621 taatgtgcca gttagtttta atgaagaaga gtttaaatta aaagaatgga ataaagaagc
     7681 attaaatgaa atttttgcag aattagaatt tagaacttta ggaaaaagat tattaggaga
     7741 agattttact attgcttcaa acaatgttca aacagatttg tttggaaata ttgtagaaag
     7801 caaaactcct aaatcagaaa aaaaacagaa agaaacaact aaagaacaag aagagtttaa
     7861 tttaattaat acaacagtag atgaagaaaa taaagcattt aaaaatataa acaatacaac
     7921 acacaattat attttgatag atagtgagga gaaaatagat aaccttatta aacaactact
     7981 acaacaaacc gaaatttgtt ttgatacaga aacaaccaat attgatgcaa ataatgcaga
     8041 actggtgggc ataagttttt catataaacc actcgaagca ttttacattc cattatctca
     8101 aaactttaca caagcacaac aaattcttga aaaatttata ccgctattta ataacacaaa
     8161 tattgtttgg ataggacaaa atattaagta cgatttatta gttttaaaat ggtataatat
     8221 tcaaatacaa ggcaatgtgt ttgatacaat gttggcacat tatgtagtag aaccagaggg
     8281 caaaagaagc atggatattt taagtgaaca gtttttacaa tatcaaccaa tacctataga
     8341 agatttgatt ggcaagaagg gaaaaacgca gggcaatatg agagatattg aagtagaaaa
     8401 aataaaagaa tatgcagcag aagatgcaga tattacctta caattaaaac aaaaaattga
     8461 accattatta aaagaaaaag aagtagaaaa agttttttac gaagtagaaa atccattagt
     8521 taaagtgctt acagcaatgg agtttgaggg aataaaaata gatgaagcct ttttaaaaga
     8581 atatagtaaa gaattaagta aagaagcaaa agcagcagaa gaggcggtat ataaaaaatc
     8641 ggaagttaga tttaatttag catcacccaa acaattagga gaagttttat ttgataaatt
     8701 aaaattagat agctcagcaa aaaaaacaaa aacaggtcag tatcaaaccg gcgaagatat
     8761 tttacaaaaa ctatcggcaa aaggccactc tattgcagaa gatattttag tgtatagaga
     8821 attaactaag cttaaatcaa cctatgtaga tgctttacca gaaataataa attcaaaaac
     8881 aggaagagtg catactactt atggacaagc agttgctgtt acaggcaggt tggcaagcaa
     8941 taaccccaat cttcaaaata ttcctataag aacagcaaaa ggaagagaaa taagaaaagc
     9001 atttatacca agagatacca atagagtttt aattagtgca gactactctc aaatagaatt
     9061 aagaattgtt gcagccataa gtaacgatga aaatatgtgt gcagctttta gagcaggaaa
     9121 agatatacat acagcaaccg ctgcaaaggt ttataatgtg gcagaaaatg aagtaaccaa
     9181 agaaatgcgt tacaaagcaa agagtgtaaa ttttggaatt atttatggac aaggagcttt
     9241 tggcttagca gataatttgg gtattagcag aacagaggca aaagaaataa ttgacaatta
     9301 taaaagagaa tttccgggca ttcaaaaata tatggacgat acaataaatt ttgcaagaga
     9361 aaatggattt gtacaaacca ttatgggaag aaaacgttgg ctgaaagata taaactcatc
     9421 taattttact gtaagaggtt ttgccgaaag aaatgctatt aacagtccaa tacaaggcac
     9481 agcagccgat atgattaaaa tggcaatgat taaaatacat actgctttac aaaaagaaca
     9541 actacaaagt aaaatgatat tacaagtaca cgatgaattg gtttttgatg tattatcctc
     9601 tgaaatagaa ctaataaagc cattaatatt aaacaacatg aaatctgcaa tggtattacc
     9661 taaccaagtt cctgtagaag cagaattggg cataggaaat aattggttag aagcccatta
     9721 gttatttaaa acaataccac tcatcattat ttctttaatt agcagtacca tttattgttt
     9781 atctagtata ttgcccaaat tcaaaaaaac aaagcctggt aatttttaaa atctaaataa
     9841 gcaggcaact ttttatagca ctttttatga aactttcttt ttctcttttg ttagtattgg
     9901 ctacaaaaac tctaatggct caagcccaaa atacacaaga gctaaataat caatcggcct
     9961 ctaataattt acaaacaatt taccataaaa ccgttcagta taaacaaaat gtttttacag
    10021 gaattgagta ttttagaaat tatggaaaaa taaatggcca cgctttttgg aatgaaaatg
    10081 aatttataaa cggaaatgtg ggctatgatg gaaacatcta tccaaacctg ccattaaagt
    10141 tagatttaat ggtagaagag ttgattacac atggggtaaa ccaaggtgtg caaataaaaa
    10201 taattagaga aaaaattgat tcttttacta taggatcagt acgttttttg cacttaacac
    10261 ccgataagaa taaagtacca gatgggtttt atgaaatatt acatagtcaa aaatatacag
    10321 tgttttgtaa aagaaataaa ttaataaaag aagttatagt acaaaacgaa ctggaaagaa
    10381 atatagaaga agaagccgtt tattttttaa aaaatcagga taatataatt gtaataaaaa
    10441 acagacaaat actatttgat ttatttagag ataaaaaaaa tcaggtatta gattatatga
    10501 gcaatttgaa tttgaatttt agaaaacaaa aagaagcttt tattgttgaa tcaataaaat
    10561 attacgagca aataaccaac taataagatg aaattttctt tacgactttt aacccttttg
    10621 gcattaataa ttttatttag ttacgcaaaa gcacaaaacg aaaataatat tacgttagat
    10681 ttaaaacgag ccagctttgc cgaatttgta cagaacgttg aaaaacaaac aaactatttt
    10741 ttttattata ataaagtaga taccgatagt gtatttatca ctatctctgt tcaaaatgct
    10801 actattgcca ctgttttaaa tactgttttt gaaaatacag aattatattt ttctatagca
    10861 gataataaaa aaatatttgt ttcaagaaac gttcaattat taaccaattt aggaacattg
    10921 ttttttaaaa caaacgagca agtaaacaaa aaagaaaaaa caattactgt aatagaagac
    10981 tctatagaag aaaccaatac caatgatgta gccatacaaa ataaattaat tgttataggc
    11041 aaaccaaatg cagcagtaag tggcaaagta ttaataaccg gccaggtaaa aaatggaaaa
    11101 aataacgaac caattgtagg tgctgttatt gcagcaggta aaacaaatgt tgtaacggat
    11161 tatttaggaa aatttacaat tagcttaaac aagggcagaa atatgcttac agcatctagt
    11221 ataggaatga gagatgccgt aagacaactc cttgttcaat catcaggcaa tattaatatt
    11281 gaaatgcaag aaaatattac tgccttaaaa ggagtggtgg ttactgcaac aaaaagaaac
    11341 actggagtaa aaacacccaa aatgggggtt gaaaaaatta ctatcaaaca attaaaacaa
    11401 caacccgttt tatttggaga agcagatgtg ctaagagcgg ttttaacact acctggtgta
    11461 acatctgttg gcgaagcaag cacaggattt aatgtaagag gcggatcggt tgatcaaaat
    11521 ttaattttat ttaatgatgc aaccattttc aatccttctc atctcttcgg tttcttttct
    11581 gcttttaacc catcggttgt taaaggagtt gaattataca aaggaagtat tccagaaaaa
    11641 tttggtggta gaatttcatc tgtattagat gtatctgttc aagagggaaa taaaaagaaa
    11701 tttggtggag caggtggcat tggcccttta acaagccatt taatgtttga gggtccaata
    11761 aaaaaagata aaggttcttt tatcattggc tttagaacaa cctactccga ttggatatta
    11821 aaaaatttaa aagatgtaag ttttaaaaat agcaaagccg catttaatga tttaagtatt
    11881 cacttatctc aacaaataaa cactaaaaac tttttgtaca taacaggcta taccagtaaa
    11941 gataatttta ggttagataa agacacaagt tatgcttata aaaacaacaa tataaatata
    12001 aagtggaaac acatttttaa aaataacctt ataagcaatg taactgctgg gtatgataat
    12061 tataactatg gtatagaaag ttctttaaaa cctctttcgg catttacatt aaaattcaat
    12121 atcaatcaat attacttaaa ggcagatttt actaaatctt atggcaatgt gcatcgtata
    12181 aactttggct ataatggtaa ttttatgaaa ttaaacccag gaagctatgc accaagtgga
    12241 aaagtgtctt tagtgaatgc aataaatatt caacaagaaa aaggaataga gaatgcaatt
    12301 tatttaggag ataaaatgga actatcctca aagcttgcag tagaagcggg tattagagta
    12361 tctgcattta ctgctattgg gtcttcaaaa gtatataagt attataatgg tttagaaaga
    12421 gatgatgcaa acattcaaga tactattttc tataaaaatg gccaaccaat aaaaacctac
    12481 tggggccctg aggtaagact ttctacacga tataccatta acgaaacaag ttctataaaa
    12541 gctggcttta gtacgcaacg ccaatatatt cacatgttat ctaacacaac tgctatttca
    12601 ccaacagata tttggaagct aagcgatact tatataaaac cacaaattgg ccaacaggtt
    12661 tctttgggtt tttataaatc atttccaaaa tcagatgttg aagtatcggt tgaagtatat
    12721 tataaatggc tacaaaattt tcttgattat aaaagcggag cattgctatt gttaaaccaa
    12781 catattgaaa cagatgtatt aaactctaaa ggcaaagctt atggtgtaga gtttatgata
    12841 aagaaaaact caggaaaatt aaatggatgg ttaagttata catactctag aacaatgtta
    12901 caaacagatg agccaacaac aagttataaa gtaaataaag gcagctatta tcctgcaaac
    12961 tttgataaac cacatagcat aaattttgtg ggcaactata agttctctca taggtttaat
    13021 gtatcgttaa caaccgttta cagtacaggt aggccaataa cattacccat tgcaatttat
    13081 aattatggtg gtggagatag agtatattat tctgatagaa accaatacag aattccggat
    13141 ttctttaggg cagatttttc tatgaacata gagggaaatc ataaaattaa aaaattagcc
    13201 cacagctcat ggtccttagg tatatacaat attacttcaa gaaaaaatcc atattctgtt
    13261 tatttcgcaa cagaaaacgg aaaaataaaa ggatatcaat tatccatttt tggttcagca
    13321 attccattta taacgtacaa ttttaaattt taaataaagg ctttacataa tgaaaaggca
    13381 tataatatat ttagtaacta tagtagcaat aataactggc tgtagagaaa attatacacc
    13441 accagaagta actacagacc ataaaatatt agtagtagaa ggttttatag atgtgaataa
    13501 agacagtagt tattttaaat tatcacgtac tcaaaaatta ggttctgttt catctgttaa
    13561 accagaagca ggagctaata tttcaattct ttcttccaac ggaacagtgc taacacaatt
    13621 taaagaaaga gccaatggcc aatatgttgt atatggtgca accctattgc caacagagtt
    13681 ttatagatta aaaattacta cttctggagc agttgtttat caatcggatt taattaaagg
    13741 aaaaataaca cctgcaatag atagtgtaac ttggaaaatt gataacgata aaaatggtat
    13801 tcagttttat gtaaactctc acgatgctgc taatgaaaca aaatattata aatgggattg
    13861 tgaagaggtt tgggaatata gggcttacta tgaatcgcag tataaatatt tgggagatac
    13921 tattattgaa ccaagattac caggagaaca aatttacaga tgttggagaa cggttttgcc
    13981 aacgcaaatt tttattaata caaccgaaaa attatcatcg gatgtaataa gtaatgaaaa
    14041 aattttattt gtaggcaaag gatctaatag gtttgatcaa cgctacagca ttttggtaaa
    14101 acaaagagca ttaaccaaag aggctttt
//
LOCUS       DAOQHP010000011        14048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2083_length_14048_cov_4.083613, whole genome shotgun sequence.
ACCESSION   DAOQHP010000011 DAOQHP010000000
VERSION     DAOQHP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_14048_cov_4.083613"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PLY81_00800"
     CDS             complement(<1..2008)
                     /locus_tag="PLY81_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNF28798.1"
                     /translation="MNKATTISKENASTHKSLQDIYIHGARMHNLKNVSVTIPRNKLI
                     VVTGVSGSGKSSLTIDTLFAEGQRRYAESLSSYARQFMERMAKPDVDYINGLCPAIAI
                     EQKVITRTPRSTVGSMTEMYDYLKLLYANIGKTVSPISNDIVKKQDVADVLNVIQQLN
                     KGTKVFIIVPFKQHAKRNTREELNILLQKGFTRVAMNNNSTTTEPEILKIEALLELDN
                     KTFKEKTASNFQVLIDRIVCEEFEEEDIHRLTDSISTAFYEGDGIVNVVTNTNKNYQF
                     NNRFELDGMRFEEPSQNLFSYNNSYGACTVCEGFGQTLDIDENLVIPDKMLSVFDGAV
                     APWKGEKMIWWKEQFIKNAVGFPIHKPVVDLTNEQYEQLWKGFGKALGIDDFFKEVEA
                     NLYKVHYRVMLSRYRGRTKCRHCKGYRLRPEALYIKINHMHIGELCEMQAKDLKVWFN
                     DLELSANESKVAQRVLLELHQRLQTLIDVGLGYLTLNRLANSLSGGESQRIQLTRSLG
                     SNLTDSLYILDEPSIGLHNRDTEKLIKVLQHLKKLGNTVVVVEHDEMMMREADYIIDM
                     GPLAAHLGGEVVATGNYHELIKNKNSLTGKYLSKELQINFNQKERKFFDKITIEGARQ
                     NNLKNITVDFPLNVICVVTGVSGSGKTTLVKQILYPALQKIKGEF"
     gene            complement(2239..5583)
                     /locus_tag="PLY81_00805"
     CDS             complement(2239..5583)
                     /locus_tag="PLY81_00805"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNF28799.1"
                     /translation="MANNKKTKRLQFSRKFTQDGISPYDLFEYDYRTSVIKNPNGEVV
                     FEMNNVEVPKQWSQIATDILAQKYFRKAGVPQQDGTLGRETSVKQVAHRMANCWKVWG
                     ERYDYFETSEDAQIFYEELVYSILNQSCVPNSPQWFNTGLHESYGITGKPQGHYYVDS
                     VDGQLKKSTNAYERPQPHACFILSVNDDLVNDGGIMDLWIREARIFKYGSGVGTNFSQ
                     IRGSGEKLSGGGTSSGLMSFLKIGDRSAGAIKSGGTTRRAAKMVCLDLDHPEVMEFIN
                     WKVEEEKKVAALVAAGYDNSYEGEAYSTVAGQNSNNSLRIPNEFFKVLEEGGDWELKA
                     RTDGRVMKKIPAKEVWNQIAYAAWRCADPGTQYDTTINEWHTCPQGGRINASNPCSEY
                     MFLDNTACNLASINLRKFFDEQTNKIDIEGVEYISRLWTVVLEISVLMAQFPSKEVAQ
                     LSYDYRTLGLGFANLGTILMLSGIAYDSEPARAIAGSLSAVMTGVTYKTSAEMASFLG
                     TFPRYEENRVDMLRVMRNHRAAAYDANEAYEGLSVKPIGINAKYCPDDLLKAATKAWD
                     DAVQMGEKYGYRNAQTTVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKIIN
                     GAVPDALKNLGYTQNQIDAIEKYAVGAASFEGAPFINPQILIAKGFTTDEVNKLNTAA
                     KSAFEIGFVFNKFILGEACLQRLGFTEEQYNDWSFSLLEALGFSDEEIDAANDYVCGT
                     MTVEGAPYLKEEHLPVFDCANKCGKKGVRYIHAHGHIKMMAACQPFLSGAISKTINLP
                     HEATVEEIAECYKLSWELGLKANALYRDGSKLSQPLSTKSDKKKKVDTTTETTQEDNK
                     EELMVNEASNIVDLSKLSVEDLLEEVTKRMNASTDTTLKRQLSRIVERKSLPAKRRGF
                     TQKAKINGQVIFLRTGEYGDGTLGEIFIDLAKEGSTVRSLMNCFAIAVSVGLQFGVPL
                     EEFVEKFVFTRFEPAGMVDHPNIKTTTSLVDFVFRALAYEYLGRTDLVHVLDKPEIGN
                     LGNDDEFNEQQELSSVRITASPKNHKHQVTSTATVKAEAGIDAVNAAAKNMQSDAPAC
                     NVCMHITVRSGTCYKCLNCGNSMGCS"
     gene            6000..6755
                     /locus_tag="PLY81_00810"
     CDS             6000..6755
                     /locus_tag="PLY81_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNF28800.1"
                     /translation="MKQDLYNTFRKNKLQRKKSFTVLIDPDKVNEQSLDKLVMLAVDA
                     KVDYFFVGGSLVISNHLDDCIKQLKAKCSIPVVLFPGSPSQVSKYADALLYLSLISGR
                     NPELLIGQHVVSAPFVKKSGLEIMPTGYMVIDGGAPTTVSYISNATPIPSDKNDIAVC
                     TAMAGEMLGMKLIYMDAGSGAKRPITESMIEKVASNIESPLIIGGGIIDAEKAYRNCS
                     AGADVIVVGNAIEKDTSLIKEIAAAVHSNSVTI"
     gene            6864..7070
                     /locus_tag="PLY81_00815"
     CDS             6864..7070
                     /locus_tag="PLY81_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28801.1"
                     /translation="MTQFILNIPTEKVQQFLITMTATGFANTIKNVLKEKNYNSFNLL
                     PLNNDLRKQHPYFDSDFFANDVFY"
     gene            7147..8598
                     /locus_tag="PLY81_00820"
     CDS             7147..8598
                     /locus_tag="PLY81_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF28802.1"
                     /translation="MDIQCTEQELCILKKITNATSQLNTKSYLIGGFVRDKIIGRPTK
                     DADIVCLNNGIELAHLTATFFKPTPNVAYFKTFGTAQLKINLHTNSSKNNSTSFEIEF
                     ASARKESYTIESRKPKVATGTLEDDQNRRDFTINALAISLNTEDFGKLIDPFNGLLHI
                     EQGIIKTPLEPSKTFSDDPLRMMRAIRFATQLNFTIVPEAFEAIIANANRIKIVSQER
                     ITDELNKIMLSKKPSIGWHLLSKSGLLEIIFPQMMALHGTEYVDNKGHKDNFYHTLQV
                     LDNICEHTQDLWLRWAALLHDIGKPATKKFEDGLGWTFHSHEIVGAKMVPKIFNQLKL
                     PLNEKMRFVKKLVALHLRPISLTKENITDSAIRRLLYDAGEDIDALMLLCNADITSKN
                     KQKVEKYLNNFKLVEERMKEVEAKDNIRNWQPPISGEHIMQTFALTPSREVGLIKDAI
                     KEAILDGKIENNYAAAYQFMLLKASELNLHPKQ"
     gene            8666..9232
                     /locus_tag="PLY81_00825"
     CDS             8666..9232
                     /locus_tag="PLY81_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28803.1"
                     /translation="MAVLKFRIYLEEDDAVYRDIAIKHTQTFELLHFTILKAFEFDSK
                     HAATFYRSNDNWQRGREITFEKYDKEYAAPPLFMQEVPIGNEIKNTNQKFIYEYDFTK
                     GWIFLVELISVNKEEKTKEEYPLITRKEGLGPQQYGTKSLLGDKFSDIEERYDLTETA
                     EGFGNEGDSEDSEIEMNEDAMDNDNEEL"
     gene            9286..10194
                     /gene="miaA"
                     /locus_tag="PLY81_00830"
     CDS             9286..10194
                     /gene="miaA"
                     /locus_tag="PLY81_00830"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNF28804.1"
                     /translation="MQLKNTVIIICGPTAIGKTKLAIEIALHLQTKIISADSRQCFKE
                     LNIGVAKPSLYELNLVEHFFINSNSIKEDTNAVSFETYALKKVNAIFQSTNYAVMVGG
                     TGLYIKAFCEGLDEIPAIPTELRNDIYTNYKANGLTWLQKQLQAKDKNFWEIAEQQNP
                     QRLMRALEVVEFTGKSINEFRKRKQQTRDFNIIKIGLETEREKLYSNINNRVDEMIQM
                     GLVEEVKNLLPFANYNALQTVGYKEIFDYLNGNCSLDFAIESIKQHTRNYAKRQITWF
                     KKDKDIKWFNQNEYNEIIAYINAVKP"
     gene            complement(10184..12640)
                     /gene="ccsA"
                     /locus_tag="PLY81_00835"
     CDS             complement(10184..12640)
                     /gene="ccsA"
                     /locus_tag="PLY81_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF28805.1"
                     /translation="MKYIGEHLLPGQIGHFFIVLSLVASLVATFAFYFASKQNILEVK
                     KSWLKFARIAFLLESISVLAVFVLLYYIISHHLFEYKYAWQHSDKQLQLEYLLSCFWE
                     GQEGSFLLWSFWHCVLGWILIWKAKDWEAGVMTVVSFAQFCLATMTLGIYFFGHKVGS
                     NPFALLRNEMDAPIFANANYLEFVKDGNGLNALLQNYWMVIHPPILFLGFASVLIPFA
                     FAFAGLMSKKHDWIKPALPWASFSAAALGLGIMLGAMWAYESLNFGGYWAWDPVENAS
                     LVPWLTLVAGLHTNLIYKHSGYSLRATYFFYIISFVFILYSTFLTRSGILGDASVHAF
                     TDLGMNWQLLLFILVFLLPAAWLFLKQYKTIPSIKKEEHTWSREFWMFVGSLVLCLSG
                     FVIISITSLPAFNRTVEHFRFLQKIFKNAFAQPDDAEFTHNQIQVFVAIIIGLLTGAV
                     QFLKYKNTPKSFIGKKFWLPVFISLIVALLIIFVGKVHYSAKGIGFMIAIHLAVFSSV
                     YAIVGNISYISFVLKGKLKSAGGSVAHLGFGLIILGILISSGKKKIISWNKTGIAVFK
                     KTAQEDPAENTTLFKGIKTDMHKYMVTYTGDKFDSSDRKRYYEIDFSPKNGSKGFKVY
                     PNIIKNNKGDGFSPNPDAKHFLTKDIFVYITSFKDDERENDTTTFRNTEINAGDTAYF
                     NNGFIVMKKANINPDSKKHLLQANETGIFLDMEVISKEGGKYIAAPGLAIKSTNEVRL
                     IYDTVLAQNLILSFNKVIDAKTGKIEIGVKENDSLTSLLTLKVLEFPMIKLLWLGVII
                     MVVGLIMSIIHRVKSKPSKV"
     gene            complement(12688..13104)
                     /locus_tag="PLY81_00840"
     CDS             complement(12688..13104)
                     /locus_tag="PLY81_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNF28806.1"
                     /translation="MKKIHVVMLVCIAAAIAVLLGFMGNVTTYETIQSAKEKQGKIVT
                     VIAKLDTATLYYDPVKNPNYLTFEAIDSLGDRMKVAYYYEKPYDMEKSERVVLKGKME
                     NNVFEIRKKDGILVKCPSKYKDDMEAAQKNLDVTTK"
     gene            complement(13221..13898)
                     /locus_tag="PLY81_00845"
     CDS             complement(13221..13898)
                     /locus_tag="PLY81_00845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF28807.1"
                     /translation="MFLEKLLKSFVNKLGYELIKDKRKFITQGLTLPMHVFTNCKLLP
                     NRNAVLPFLPKQMVIAEVGVAYGEFSREIIDVANPSKFYAIDYYQGEEFWGRNTFEKN
                     KLSHEDYIKQQFNKEIEQGIFYTKKGLSWDVLNTFSDNFFDYVYLDAGHDYESVKKDI
                     EVLLKKVKNGGVIQFNDYIIYDWVSKLPYGVVKAVDELLQNNQHEVLYYCLHKGGFND
                     IVVKINK"
BASE COUNT         4786 a         2426 c         1945 g         4891 t
ORIGIN      
        1 caaactctcc tttaattttt tgtaaagcag gatataaaat ttgttttacc aaagtagttt
       61 taccactacc acttacacct gttaccacac atattacatt taaaggaaaa tctacagtaa
      121 tattttttaa gttattctgc cttgctcctt caattgttat tttgtcaaaa aattttcttt
      181 ccttttggtt aaaattaatt tgaagttctt tacttaaata tttaccagtt aatgagtttt
      241 tatttttaat taactcatgg taattacctg ttgctacaac ttctccacca agatgggcag
      301 ctaatggccc catatcaatt atataatcag cctctctcat catcatttca tcatgttcta
      361 ctacaacaac tgtatttcct agttttttta agtgttgtaa aactttaatc agtttttctg
      421 tatctctatt atgtaaacca atactaggct catctagtat atataaagag tcggtaagat
      481 tagagcctaa gcttcttgtt aactgtattc tttggctttc tcccccactt aaactgtttg
      541 ctaatctgtt taaagttaag taacccaatc ctacatcaat taaagtttgt aaacgctggt
      601 gtagttccaa taaaactctt tgtgcaactt tgctttcatt ggcagataat tctaaatcat
      661 taaaccatac ttttaaatct tttgcttgca tttcgcaaag ctctccaata tgcatatggt
      721 taattttaat atacaatgct tctggcctta acctatatcc tttacaatgc ctgcattttg
      781 ttctacctct atacctgcta agcataactc tataatgaac tttatataag tttgcttcta
      841 cttctttaaa aaaatcatca ataccaagtg cttttccaaa acctttccat agttgttcat
      901 attgttcatt agttaaatct accacaggtt tatgaatagg aaatccaact gcatttttta
      961 taaactgttc tttccaccaa atcattttct ctcccttcca aggtgcaact gcaccatcaa
     1021 aaacgcttag cattttatct ggtattacta agttttcatc aatatctaaa gtttgcccaa
     1081 agccttcgca aacagtacag gcaccatagg agttattgta agaaaataaa ttttgcgatg
     1141 gctcttcaaa cctcattcca tctaactcaa acctattatt aaattggtaa tttttgtttg
     1201 tattagtaac cacattaaca attccatctc cctcataaaa ggctgtagaa atactatctg
     1261 ttaatcggtg tatatcttct tcttcaaatt cttcacaaac aattctatca attaatactt
     1321 gaaaatttga ggcagttttt tctttaaatg ttttattatc cagctccaat aatgcttcaa
     1381 tctttaatat ttctggctct gttgtggtag aattgttatt catagcaact cttgtaaaac
     1441 ccttttgtag caatatgttt aactcttctc ttgtatttct ttttgcatgt tgtttaaatg
     1501 gtacaataat aaaaacttta gtgcctttat taagttgctg aataacattt aatacatctg
     1561 ccacatcctg tttctttact atgtcattac taattgggct aacagtttta ccaatatttg
     1621 catacaatag tttcaaataa tcatacattt ctgtcatgct gccaacagta cttcttggag
     1681 tacgtgttat aactttttgt tcaattgcta tagcaggaca taagccatta atataatcca
     1741 catctggttt tgccatacgt tccataaact gccttgcata agagcttaaa ctctctgcat
     1801 aacgcctctg cccttctgca aacaaagtat caattgtaag cgatgatttg ccactgccac
     1861 taacgccagt taccacaatt aatttatttc ttggaatagt tacagaaaca ttttttaaat
     1921 tatgcatcct tgcaccatga atatatatat cctgcaaaga tttatgtgta ctggcattct
     1981 ctttgcttat ggttgtggct ttattcatac aatcaaaata acaattttat tttcaaaaca
     2041 atgtaatggg ttacaccgaa atatttttta atgtatagtt atcaggtgta gccaacaatg
     2101 tagcactgta tgtgtcacac ccttcctctg ttttttctct atttaacttt taatagtaag
     2161 aaaattttgg taataattac aaataaaaaa gcagagatac gttttacata tctctgcctt
     2221 cattacttaa aatatttttt aactgcaacc catactgttg ccacagttta agcatttata
     2281 gcaagtgcca cttcttacag taatgtgcat acatacatta caagcaggtg catcgctttg
     2341 catatttttt gcagctgcat taactgcatc tatgccggct tctgctttta cagttgctgt
     2401 tgaggttact tggtgtttgt gattttttgg agaagctgtt attctaacac tactcaactc
     2461 ctgttgttcg ttaaattcat catcattacc taagtttcca atttctggtt tatctaaaac
     2521 atgcactaaa tctgttctgc ctaaatattc ataagccaat gctctaaata caaagtcaac
     2581 taaagaggta gtagttttaa tgtttggatg atctaccatt cctgctggtt caaaccttgt
     2641 aaacacaaat ttttctacaa attcttctaa aggcacacca aactgtaaac caaccgatac
     2701 agcaatggca aagcagttca ttaagcttct aactgttgat ccttctttag ccaagtcaat
     2761 aaatatttcc cctaaagtgc catccccata ttctcctgtt cttaagaaaa taacttgtcc
     2821 attaattttt gctttttgtg taaagcctct tctttttgct ggtaaagatt tcctttcaac
     2881 tattctagaa agttggcgtt ttaaagttgt atcggtagag gcattcattc ttttagtaac
     2941 ctcttctaat aaatcttcta cagatagttt agaaagatct acaatattag aagcttcgtt
     3001 aaccattaat tcttctttat tgtcttcttg tgttgtttct gtagtagtat ctactttttt
     3061 ctttttgtct gatttagtac ttaacggttg cgataactta cttccatctc tatataaagc
     3121 gtttgctttt aaaccaagtt cccagcttag tttatagcat tctgcaattt cttctacagt
     3181 tgcttcgtgt ggtaaattaa ttgtttttga aattgctcca cttaaaaatg gttggcaggc
     3241 tgccatcatt ttaatatgtc catgtgcatg tatataacga actccttttt taccacattt
     3301 attagcacaa tcaaaaacag gtaaatgctc ttcttttaaa tatggtgctc cttctacagt
     3361 cattgtgcca catacataat cattagcagc atctatttct tcatcgctaa aacctaaagc
     3421 ttctaataag ctaaagctcc agtcgttata ttgttcttct gtaaatccta atcgttgtaa
     3481 acaagcttct cctaaaataa atttattgaa tacaaaacca atttcgaaag ccgattttgc
     3541 agcagtattt agtttattaa cttcatcggt tgtaaatcct tttgcaatta atatttgagg
     3601 attaataaaa ggtgctcctt caaaacttgc agcaccgact gcatattttt caattgcatc
     3661 tatttggttt tgtgtatatc ctaaattttt taaagcatca ggaacagcac cattaattat
     3721 tttaaaatat ccaccgccgc taagtttttt aaatttaacc aaagcaaaat ctggctctac
     3781 accagtggta tcacaatcca ttactaaacc gatagttcct gtaggtgcta ttactgtggt
     3841 ttgagcattt ctataaccat atttttcgcc catttgtact gcatcatccc aggcttttgt
     3901 tgcagctttc aataaatcat cgggacaata ttttgcatta atgccaattg gttttacact
     3961 aagcccttca taagcttcat tagcatcgta agctgctgca cggtggttac gcattactcg
     4021 taacatatct accctatttt cttcgtaacg aggaaaggtg cctaaaaacg aagccatctc
     4081 tgccgatgtt ttataagtaa caccagtcat aactgcactt aaagaacctg caatagctct
     4141 tgctggttca ctatcataag caataccact aagcattaaa atagttccaa ggtttgcaaa
     4201 gccaaggcct aatgttctat aatcgtaact taattgggct acttctttac ttggaaactg
     4261 agccattaat acagagattt ccaaaacaac tgtccataac ctgcttatat attctacccc
     4321 ttcaatatct attttattgg tttgttcatc aaagaatttt cttaaattta tagaggctaa
     4381 attacatgca gtattatcta aaaacatgta ttcgctacaa gggttgcttg cattaattct
     4441 tccaccttgt gggcaagtat gccattcgtt aattgtagta tcgtattgtg ttcctgggtc
     4501 tgcacaacgc catgcagcat aagcaatttg gttccatact tctttagcag gaattttttt
     4561 catcaccctg ccatctgttc ttgcttttaa ttcccaatct ccgccttctt ctaatacttt
     4621 aaaaaattcg ttaggtattc ttaatgagtt attagagttt tggcctgcta ctgtagagta
     4681 agcttctcct tcatagctat tgtcatatcc agcagcaacc aaagcagcta ctttcttttc
     4741 ttcttctacc ttccagttaa taaattccat cacttccgga tgatctaaat ctaaacaaac
     4801 cattttagct gctctacggg ttgtacctcc actttttatg gctccagcag atctatctcc
     4861 aatttttaaa aaactcatta aaccactaga agtaccacca ccacttaatt tttctccact
     4921 accacgtatt tgagaaaaat tagttccaac gccagaacca tatttaaaaa ttcttgcttc
     4981 tctaatccat aaatccataa taccaccatc attcactaaa tcatcattaa cacttaaaat
     5041 aaaacaagca tgtggttgtg ggcgttcgta tgcattggta gattttttta attgtccgtc
     5101 cactgaatca acatagtagt gaccttgtgg cttacctgta attccataac tttcatgtaa
     5161 accagtatta aaccattgcg gactattagg aacacaactt tgatttaata tactataaac
     5221 taattcttcg taaaaaattt gtgcatcttc tgaggtttca aaataatcgt atcgttctcc
     5281 ccaaactttc caacagtttg ccatacgatg ggcaacctgt tttacacttg tttctctacc
     5341 caaagtgcca tcttgttgtg gaacacctgc ctttctaaaa tacttttgtg caagaatatc
     5401 tgtagctatt tggctccatt gcttaggaac ttctacatta ttcatctcaa aaactacttc
     5461 tccattagga tttttaatta cactggtacg atagtcgtat tcaaacagat cgtatgggct
     5521 tataccatct tgtgtgaatt tacggctgaa ctgaagacgt ttggtttttt tgttgttagc
     5581 catttgtgat agaatttatg ggtggttaat gatgttttat aaattttgcc tttctaaaca
     5641 aaaaagttta gtggttaacg aaaatatttg ttgcacttca attttccgta cctataaaat
     5701 ccacaggttg tggaaaagga atgttaattt tttggtaact caaagccact ttgagtttgc
     5761 taagttttta acatttgagg tagaataact taaaaaatat ttatggaaaa ttcatctatt
     5821 tctcataata aagcatattg gaacaaatta ctttgtttat ataattgtta ttaaaccagt
     5881 taattaaatt tattcttctt aaaagttaat tttatagcat ttattgtata tttttaacca
     5941 aacttggaat aagcaatact tttttgcatt tttgcaatac catttattta actaattgca
     6001 tgaagcagga tttatacaat acattcagaa aaaataaatt acaaagaaaa aaatctttta
     6061 ctgtacttat cgaccctgat aaagtaaatg aacaatcttt agataagctt gtaatgcttg
     6121 cagtggatgc caaagtggac tatttttttg tgggtggatc gttggttata tccaatcatt
     6181 tagatgattg tataaagcaa ttaaaagcaa aatgttctat tcctgttgta ctttttcctg
     6241 gttcaccttc tcaagttagt aagtatgccg atgctctttt atatctttct ttaatttctg
     6301 gacgtaatcc ggaattgtta attggccagc atgttgttag tgctcctttt gttaaaaaaa
     6361 gtgggttaga gattatgcca actggttata tggttattga tggtggagct cctacaacag
     6421 tttcgtatat ttcaaatgcc acacccatac cttctgataa aaatgatatt gcagtttgta
     6481 cagctatggc tggtgaaatg cttggcatga aattaattta tatggatgct ggtagtggtg
     6541 ctaaacgccc aattactgaa tctatgattg aaaaagtggc aagcaacatt gaatctcctt
     6601 taattattgg tggcggaatt attgatgcag aaaaagcgta tagaaattgt agtgcaggtg
     6661 ccgatgttat tgttgtagga aatgcaatag aaaaagacac ttcacttata aaagaaattg
     6721 ctgctgctgt gcatagtaat tctgttacta tataatcaat tctttaaagc aaattttgta
     6781 acaaaacctt aacgctaaaa agtatgttgt agcactaatt gtgttgtcta tataggtatt
     6841 ataaacctat taaattaaat tctatgacac agtttatctt aaacatacct actgaaaaag
     6901 tgcaacagtt tttaattaca atgactgcaa caggttttgc taataccata aaaaatgtat
     6961 taaaagaaaa aaattacaac agttttaatc tactaccatt aaataacgat ttgcgtaaac
     7021 aacatcctta ttttgattca gatttttttg caaacgatgt tttttattaa tccatatttt
     7081 ttatattaat tgaattaaaa attgtaaaaa caaattctta caatttgaat attatctttg
     7141 ccctgtatgg atatacagtg cacagaacaa gagttatgta ttttaaaaaa aattacaaat
     7201 gccactagtc aattaaatac aaaaagttat ttaattggtg gatttgtacg agataaaatt
     7261 attggaagac ctaccaaaga cgcagatatt gtttgtttaa ataatggtat tgaattagca
     7321 cacttaacag ctacattttt taaacctacc cccaatgtag cttactttaa aacatttggt
     7381 acagcacagt taaaaattaa ccttcataca aattcatcta aaaacaattc tacaagtttt
     7441 gaaatagaat ttgctagtgc tagaaaagaa agctacacta ttgaaagtag aaaaccaaaa
     7501 gtagcaacag gcactttaga agatgaccaa aacagaagag attttactat taatgcttta
     7561 gcaattagct taaatacaga agattttggg aaattaattg acccatttaa tggattatta
     7621 catatagagc aaggtattat taaaacacca ttagaaccat ccaaaacatt tagcgatgac
     7681 ccattaagaa tgatgagggc tatacgattt gctacacaat taaactttac aattgtaccc
     7741 gaagcttttg aggcaattat tgccaatgcc aatcgtatta aaatagtttc gcaagaaaga
     7801 attacagatg aattaaataa aataatgttg agcaaaaagc catctattgg ctggcactta
     7861 ttaagcaaat ctggtttgtt agaaataatt tttccgcaaa tgatggcttt acatggaaca
     7921 gaatatgtag ataataaagg gcataaagat aatttttatc acaccttaca agtacttgat
     7981 aatatttgtg aacacacaca agatttatgg cttagatggg ctgcattgct acatgatatt
     8041 ggaaagcctg caactaaaaa atttgaagat ggtttaggct ggacatttca ctctcatgag
     8101 attgttggtg caaaaatggt tcctaaaatt ttcaaccaat taaaacttcc attaaatgaa
     8161 aaaatgaggt ttgtaaaaaa attagtagca ctgcatttga ggccaatttc tttaacaaaa
     8221 gaaaatatta ccgatagtgc cattagaaga ttattgtacg atgcaggaga agatattgat
     8281 gctttaatgc ttttgtgtaa tgcagatata acgagtaaaa acaagcaaaa agtagaaaaa
     8341 tatttaaaca attttaagct tgttgaagaa cgcatgaaag aagtagaagc aaaagataat
     8401 atacgtaatt ggcaacctcc tataagtgga gagcatatta tgcaaacctt tgctttaact
     8461 ccatctagag aagttgggct tattaaagat gctataaaag aagctatttt agatggtaaa
     8521 attgaaaata attatgctgc tgcataccaa tttatgttgc taaaagcaag cgaattaaac
     8581 ctgcatccaa agcaataact agttttttat tattagcaaa tttgtttaaa aattttcaac
     8641 ttatatttac aaacaaacca aacacatggc agttttaaaa tttagaattt atttagaaga
     8701 agacgatgct gtttatagag atattgcaat aaaacatacc caaacttttg aacttcttca
     8761 ttttactatt ctaaaagctt ttgaatttga tagtaaacac gctgcaactt tttacagaag
     8821 taatgacaat tggcaaaggg gtagagaaat aacttttgaa aaatatgata aagaatatgc
     8881 tgcacctccc ttatttatgc aagaagttcc aataggcaat gaaataaaaa ataccaatca
     8941 aaaatttatt tatgaatacg attttaccaa aggttggata tttttagttg aactaataag
     9001 cgtaaataaa gaagaaaaaa ctaaagagga gtatccttta ataactcgta aagaaggatt
     9061 aggccctcaa caatatggca ccaaaagcct tttaggcgat aagttttctg atattgaaga
     9121 gagatatgat ttaaccgaaa cagcagaagg ttttggcaac gaaggagata gtgaagattc
     9181 ggaaatcgaa atgaatgaag atgcaatgga taatgataat gaagaattat aacccgttat
     9241 aaaacatcaa taatattgat tgtaatttta acttattaat ataacgtgca gcttaaaaat
     9301 actgtaatta taatttgtgg ccctacagca attggtaaaa caaaattagc cattgaaatt
     9361 gctttacact tacaaacaaa aataatttct gccgatagca gacaatgctt taaagagtta
     9421 aatattggtg ttgcaaaacc aagtttatac gaattaaatt tagtagagca tttttttatt
     9481 aactctaatt caataaagga agataccaat gcagtcagtt ttgaaactta tgctttaaaa
     9541 aaagttaatg caatatttca atctacaaat tatgctgtaa tggttggtgg aactggttta
     9601 tatataaaag ccttttgcga aggattagat gaaattcctg caataccaac agaattgaga
     9661 aatgatattt atacaaatta taaagccaat ggcttaacat ggcttcaaaa acaactacaa
     9721 gcaaaagata aaaacttttg ggaaattgca gaacaacaaa atccccaacg tttaatgaga
     9781 gcattagaag tagttgaatt tactggaaaa tctattaatg aatttagaaa acgtaaacaa
     9841 caaaccagag attttaatat aattaaaatt ggattagaaa cagaaagaga aaaattgtat
     9901 agcaatatta ataatcgtgt agatgaaatg atacaaatgg gcttagtaga ggaagtaaaa
     9961 aatttattac cttttgctaa ttataatgct ttgcaaacag taggctataa agaaatattt
    10021 gattatttaa atggaaattg ttcattagat tttgcaatag aaagtattaa acaacacacc
    10081 agaaactacg ctaaaaggca aattacctgg tttaaaaaag ataaagatat taaatggttt
    10141 aaccaaaatg aatataatga aattatagct tatataaatg cagttaaacc ttagaaggtt
    10201 ttgatttaac tctatgtata atactcatta ttaacccaac aaccattata ataactccta
    10261 accataacaa ttttatcatc ggaaattcta aaacttttaa agtaagtaaa gatgttaatg
    10321 aatcattttc tttaacacca atttcaattt tacctgtttt tgcatctata actttattaa
    10381 aactcaatat taaattttga gctaatactg tatcatatat taatcttact tcgttggttg
    10441 atttaatggc taaaccaggt gctgcaatat atttacctcc ttctttactt ataacctcca
    10501 tatctaaaaa tatacctgtt tcgttagctt gtaacaaatg ctttttagaa tcaggattta
    10561 tgttagcctt tttcattaca ataaagccat tgttaaaata ggcggtatct cctgcattta
    10621 tttctgtatt tctaaatgtt gttgtatcat tctctctttc atcatcttta aatgaagtga
    10681 tataaacaaa gatatctttg gttaaaaaat gttttgcatc aggattagga gaaaaaccat
    10741 cccctttatt attttttatg atattagggt aaactttaaa tcctttacta ccatttttgg
    10801 gagaaaagtc aatttcataa tatcgttttc tatcgctact atcaaactta tctcctgtat
    10861 aagtaaccat gtacttatgc atatctgttt taataccttt aaaaagtgta gtattttcag
    10921 ctgggtcttc ttgtgctgtt ttcttaaata ctgcaattcc tgttttgttc caactaatta
    10981 tttttttctt tccactcgaa attaatatac ctaaaattat taacccaaat ccaagatgtg
    11041 caacactacc tccagccgat tttaattttc cttttaaaac aaaagatata taactaatat
    11101 taccaactat tgcatatact gaagagaata cagccaaatg tattgcaatc ataaaaccaa
    11161 ttcctttggc tgaataatgc actttaccaa caaaaataat tagcaatgca acgattaagg
    11221 aaatgaaaac aggtagccag aattttttgc caataaatga tttgggtgtg tttttatatt
    11281 ttaaaaactg aacagcacca gttaacaacc ctataataat agcaacaaat acctgaattt
    11341 gattgtgtgt aaactctgca tcatctggct gtgcaaaagc gtttttaaat attttttgca
    11401 aaaacctaaa atgttctaca gttctattaa atgcaggtaa tgaggtaatg gatatgatta
    11461 caaaaccaga taaacacaaa accaacgagc caacaaacat ccaaaattca cggctccaag
    11521 tgtgctcttc ttttttaata gaaggtattg ttttgtattg ttttaagaat aaccaagctg
    11581 ccggtaataa aaaaactaga ataaataaaa gcaattgcca attcattcct aaatcagtaa
    11641 aagcatgtac agacgcatct cctaaaatac cacttcttgt taaaaaagta gagtataaaa
    11701 taaaaacaaa agagataatg taaaagaaat aagttgctct taaactataa ccactatgtt
    11761 tataaattaa attggtgtgt aaaccagcaa ctaaagtaag ccaaggaacc aatgatgcat
    11821 tttcaacagg atcccaagcc caataacctc caaaatttaa actttcataa gcccacatgg
    11881 cacctaacat aatacctaaa ccaagtgctg ctgctgaaaa agatgcccaa ggcaatgcag
    11941 gttttatcca atcgtgtttt ttgctcatta atcctgcaaa agcaaatgca aaaggtatta
    12001 gtacagatgc aaatcctaaa aacaatatag gtggatgaat aaccatccaa taattttgta
    12061 acaatgcatt taagccattg ccatctttta caaattctaa ataattagca ttggcaaaaa
    12121 tgggtgcatc catttcattt cttaataatg caaacggatt gctacctact ttatggccaa
    12181 agaaatatat acctaaagtc attgtagcta aacaaaattg ggcaaaactt accacagtca
    12241 ttacaccagc ttcccaatct ttcgctttcc aaattaaaat ccatcctaat acacaatgcc
    12301 aaaaactcca aagtaaaaaa cttccttctt gtccttccca aaaacaactt aataaatact
    12361 ctaactgtaa ttgtttatcg ctgtgttgcc aagcgtattt atattcaaat aaatgatgag
    12421 agatgatata atataataaa acaaaaacag ccaataccga aattgactct aataaaaaag
    12481 caatacgtgc aaactttaac cagctctttt ttacctctaa aatattttgc ttactggcaa
    12541 agtaaaatgc aaaagttgct actaaggatg ctactaaaga aagtacaata aaaaaatgcc
    12601 ctatttgtcc tggtaaaaga tgctctccaa tatatttcat tcaaagtggt tgttagtttt
    12661 aattaaatag cttacaaaaa actcaaatta tttagttgta acgtctaaat ttttttgagc
    12721 agcttccata tcatctttgt atttactagg acattttaca agtattccgt ctttttttct
    12781 tatttcaaaa acattatttt ccattttacc ttttaaaact actctttcac ttttttccat
    12841 atcataaggt ttttcataat agtaggctac tttcattcta tcgcctaaag agtctatagc
    12901 ttcaaaagtt aaataattag gattttttac agggtcgtaa tataaagttg cagtatctag
    12961 ttttgcaata acggtaacaa ttttaccttg tttttctttg gcagattgaa tagtttcgta
    13021 ggtagtaaca ttgcccataa aaccaagtaa aacagcaatg gctgcagcaa tgcaaaccaa
    13081 cataacaaca tgtattttct tcatgttcta attttttgca aatttaagtt aactgattta
    13141 ctaattaagg attaaaaaac caaaaagaat gtgaaataca cattggaagt tcttaaatta
    13201 aactcacctt ctctaactta ttacttattt atttttacca caatatcatt aaacccacct
    13261 ttgtgtaggc aataatataa aacctcgtgt tgattatttt gtaataactc atctacagct
    13321 tttacaaccc cataaggcaa tttacttacc caatcataaa taatatagtc gttaaactga
    13381 atgacacctc cattttttac cttttttaaa agcacttcta tatctttttt tacactttca
    13441 taatcatgcc ctgcatctaa ataaacataa tcaaaaaaat tatccgaaaa ggtatttaac
    13501 acatcccacg ataatccttt tttagtatag aaaatacctt gttcaatctc tttattgaat
    13561 tgttgtttta tataatcttc atgcgatagt ttattttttt caaatgtatt tcttccccaa
    13621 aattcttcgc cttggtaata atctattgcg taaaatttac ttggatttgc aacatctatt
    13681 atttccctgc taaattcgcc atacgccact cccacttctg ctataaccat ttgtttagga
    13741 agaaatggta atactgcatt tctattaggc aatagtttac aattagtaaa aacatgcatt
    13801 ggtaatgtta aaccctgtgt tataaatttt cttttatctt taattaattc atatcctaat
    13861 ttatttacaa aagattttaa taatttttct aaaaacatag aaattaaata tggttgaagt
    13921 aaaatgtaaa tatctattta tttaaacaaa ttgtagcata aaaaaagcct acagagaatc
    13981 tgtaagctat tttttgtgat cccgctggga ctcgaaccca gggcccatac attaaaagtg
    14041 tattgctc
//
LOCUS       DAOQHP010000012        13849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2129_length_13849_cov_4.130926, whole genome shotgun sequence.
ACCESSION   DAOQHP010000012 DAOQHP010000000
VERSION     DAOQHP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_13849_cov_4.130926"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLY81_00850"
     CDS             <1..764
                     /locus_tag="PLY81_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF28808.1"
                     /translation="GGVNFEPYKAKLFESIGKKIDTIELFPASEGFFAFQNSQQEEGL
                     LLNTNSGIFYEFIPAAEIFTENPTRLCLQDVKLGENYALIINSNAGLWGYNIGDTVKF
                     VSLNPYKIIVSGRIKHFISAFGEHVIAEEVEHALLKAANEENIKIIEFTVAPNVAQGK
                     GKSFHEWFIEFENLPKNLPSFIEKVDKNLRDKNVYYDDLIKGNILQTLHINCIRKNGF
                     IDYMKSIGKLGGQNKVPRLSNDRKIAEELNGFINN"
     gene            complement(913..1875)
                     /locus_tag="PLY81_00855"
     CDS             complement(913..1875)
                     /locus_tag="PLY81_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF28809.1"
                     /translation="MLKVGVFGVGHLGKFHLNNWKEIEGIKIVGFFDPNNDAAKEVTA
                     TYGIKRFLDEDKLIDACDIIDIVVPTDWHFEVCSKAVRKGKHVFVEKPLCNTIPEAKA
                     LVEMVREANIKMQVGHVERFNPAYLALKDLSLNPMFIEVHRLAQFNPRGTEVSVILDL
                     MIHDLDIILSLVKSDVKTISANGVAVMTDTPDIANVRIEFNNGCVANLTSSRISMKKM
                     RKMRLFQKDSYIGIDFLEKKTEIIKLKEPTDKNVFAFDVETPKGKKTIAVANPTITEA
                     NAIKLELEAFVDSILNNKQTIVNEIDGYLAMEVAHQILNKINNN"
     gene            complement(1998..2558)
                     /locus_tag="PLY81_00860"
     CDS             complement(1998..2558)
                     /locus_tag="PLY81_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF28810.1"
                     /translation="MQLLKNIKNNWTLFLDRDGVINYEKENSYIFNWEEFRFYPEVKE
                     ALKILQQYFATIVVVTNQRGIGKQLMSEGDLQNIHQNMVQEIQNAGGRIDKIYFCSDL
                     ENTSPCRKPNTGMALQAKKDFPAMDFTKSIMVGNKLSDMQFGKNAGMHTVFVATTHPQ
                     TPYPHATIDARFKNLLEFALAIQHAK"
     gene            complement(2621..3193)
                     /locus_tag="PLY81_00865"
     CDS             complement(2621..3193)
                     /locus_tag="PLY81_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNF28811.1"
                     /translation="MQQKIKSIIQASITTKQEILNNNSLIQTIDDCVNSIVQAFNNGN
                     KVLFCGNGGSAADAQHLAAEFSGRFYKDRKALPAEALHCNTSYLTAVANDYSYDAIYA
                     RLLDGIGNSGDILIGLTTSGNSKNIIQAFEVANAKNITTIAFTGNTGGDIKGLTKYLL
                     NVPSNNTPRIQESHIMLGHIICELVEEKLF"
     gene            complement(3213..4238)
                     /locus_tag="PLY81_00870"
     CDS             complement(3213..4238)
                     /locus_tag="PLY81_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNF28812.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPYSDEFGGAILNATISLNAYASI
                     QLEKTGGITLQALDRNEIQQFDFSKTLPINGTVDLLKGVYNRIQKDYGFAHQNFTLST
                     FVDAPAGSGLGTSSTLVVAIIGAFVEMLKLPLGDYDIAHYAYDIERNDLSLAGGKQDQ
                     YAATFGGVNFMEFYNNDKVIVNPLRIRSEYISELENNLVLYFTATSRESATIIKEQQK
                     NVVNKNEKSIEAMHQLKEQAKMMKEALLKGKLNEFGEILDFGFQQKRKMANNISNSNI
                     ENIYDAAKKAGATGGKISGAGGGGFMIFYCPQNTRYKVIETLNSFGGVVKPYAFTKNG
                     LKTWSVH"
     gene            complement(4272..5087)
                     /gene="panB"
                     /locus_tag="PLY81_00875"
     CDS             complement(4272..5087)
                     /gene="panB"
                     /locus_tag="PLY81_00875"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNF28813.1"
                     /translation="MSIHKEVKKITTNTLQKMKQSGEKISMLTAYDFSFAKIIDNAGI
                     DIILVGDSASNVMAGHETTLPITLDQMIYHAQSVVRAINRCLVVVDLPFGTYQSNSDI
                     ALASAIRIMKETGGHSVKLEGGAEVVESVRKIVGAGIPVMGHLGLTPQSIYKFGTYTV
                     RAKEQAEADKLLSDAKLLQEAGCFAVVVEKIPAQLAKQVSETIDIPTIGIGAGPYCDG
                     QVLVMHDMLGINTEFKPRFLRQYASLHDITFNAVQQYITDIKSLNFPNQTESY"
     gene            complement(5171..6673)
                     /locus_tag="PLY81_00880"
     CDS             complement(5171..6673)
                     /locus_tag="PLY81_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28814.1"
                     /translation="MISSKKMLHPITILLLVTIIAAIATWLLPAGTYNKLSVSEKNFV
                     INSNGINTHVPLTQNTLDSLHIIIPLEKFTNGDITKPVSIPNTYQATKSNRTTFIQLL
                     SSPIKGIYDAIDIVLLILVMGGFIYVFNETGAMLKGISYLSFKLKGREQFLIIILTAL
                     FSFGGSSYGMAEETLVFYPVLVPLFLAAGYDLLIPVAVIFAGSQLGTLSSFSNPFATI
                     IGSNAAGLNWIDGIYERILMYIISTALLIWYILRYAKKIKANPANSLVLKYDGNLHYN
                     YSATVNNEDINNTLTLRTKLLLLLFASAFIIMIAGVIFLKWWLLEITVLFFGASLLLF
                     FINKTKEAEFIQQFIKGAESLLGVAFIVGIARGITIVLNDGNISDTLLFYTAKITGSF
                     PPAFFILILLGLFIILTLFISSSSGMAVLTMPILGALALIVHVPGREIVNSYLYGMGI
                     MGFITPTGLFLPSISMVNISTKTWFKFITPFLVILFILCAIALLIGIYIK"
     gene            6844..6942
                     /gene="ffs"
                     /locus_tag="PLY81_00885"
     ncRNA           6844..6942
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLY81_00885"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7029..7679
                     /gene="fsa"
                     /locus_tag="PLY81_00890"
     CDS             7029..7679
                     /gene="fsa"
                     /locus_tag="PLY81_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNF28815.1"
                     /translation="MKFFVDTANLNQIKEANDLGILDGVTTNPSLMAKEGVKGEEAIM
                     NHYKTICEMVDGDISAEVLSVTFNEIIEEGKKLAAIHPNIVVKVPMIKDGIKAIKWFT
                     DNGIKTNCTLIFSAGQAILAAKAGATYISPFLGRIDDSGWDGVQLIEEIAQIYAVQGF
                     KSEILAASIRNPNHIIRCAAAGADVCTCPLDSILGLLKHPLTDIGLAKFLEDAKKM"
     gene            complement(7737..9569)
                     /gene="aspS"
                     /locus_tag="PLY81_00895"
     CDS             complement(7737..9569)
                     /gene="aspS"
                     /locus_tag="PLY81_00895"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNF28816.1"
                     /translation="MFRSRTCGELTIKNVGEIVTLAGWVQTIRKFGAITFIDLRDRYG
                     ITQILFGENLNNQLEENSLGREFVLQVNGTVTERSNKNKNIATGEIEVTVNNFTILNK
                     SETPPFTIQDETDGGDDLRMKYRYLDLRRNAVKKNIELRYAVNRSARNYLHENGFIDI
                     ETPFLIKSTPEGARDFVVPSRMNVGQFYALPQSPQTFKQLLMVSGYDKYYQIVKCFRD
                     EDLRADRQPEFTQIDCEMSFVEQEDILNMFEGLIKSIFKEVKNIDYTSVVERMTWQEA
                     MWQYGNDKPDIRFGMQLCNLKFPAHSFPEKNNISDLIAADLAVFNDAETVVAIAVPDC
                     SEFTRKQTDELTEWVKRPQIGMKGLVFIKCNTDGTFKSSVDKFYSEEKLKAIAQAANA
                     KAGDLILILAGQEERTRKAISELRLEMGKRLGYRKEDEFKLLWVVDFPLFEYAEEENR
                     WVARHHPFTSPKPSDIETMIHNNPAIENAADYLKHPYANIKANAYDMVLNGNEIGGGS
                     IRIYQKELQQKMFAALGMNEEEQQHKFGFLLGAFEFGAPPHGGIAFGFDRLCSILGGS
                     ESIRDFIAFPKNNSGRDVMLDAPSSIDTKQLNELQIKLTPKEIN"
     gene            complement(9772..10455)
                     /locus_tag="PLY81_00900"
     CDS             complement(9772..10455)
                     /locus_tag="PLY81_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNF28817.1"
                     /translation="MRRVYLALLVSCSMFFSSCDTLKSVADSILTERDAISAIKEMLT
                     IGSNGNALISKDVLMNAILPKDVNTVLQKLQQMGLSKEIDKFTNTLTNAATQTATNSI
                     PVFADGIKRMNITDAINIVKNGGTAATDYFRTTIGNDLRTTVTPIMKTALDQYQVTKE
                     WDKLVAPAKIFLGNKINLNLNLDNLLAGVVTNAMFNKIAEQEVNIRTKAEARTSPLLQ
                     RVFGKNWNN"
     gene            10566..12503
                     /locus_tag="PLY81_00905"
     CDS             10566..12503
                     /locus_tag="PLY81_00905"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNF28818.1"
                     /translation="MIRLLLFISIFFYKVNAQEMENRTVSNLSLTNTILTAPKFNTPA
                     ISKASNNFHIHYLKFKLKVNPAIRYIDGSVDIYFIPNILTDSIELDLSYQLKVDSIVF
                     NNQKLSYIQHPNNTLIIYFQTALVAAQNYSMNIFYKGVPGDSGYGSFIQTTHNNTPVI
                     WTLSEPFGSKDWFPCKNELSNKIDSVDMFITHPSVYKATANGVLQYEQTNGGNTTTFF
                     KHRYPIATYLIAFAVSNYSVQQIKMKIGIDTLQYVSYIYPESVADFQNLIIPTINALQ
                     LFSNTFGTYPFIKEQYGITQFGWGGGMEHQTNSFLSAPDTNLMVHELAHQWFGNKVTC
                     HSWQHIWLNEGLATFAANYYFEKVDSAKLLSFLTTQLNNITKLPDGKIIVSDTFNVGS
                     IFNSRLSYDKAAFMLRMLRYTLGDSIFFKAVFNYINDPLLQYNFVTTNHFIQHVEEVA
                     KQDLSWFFNQWLNGEGFPTFNVSWSNNNFNWVKVIVNQTTSHSSVPFYKTVLPLTFKN
                     NHQQKTIKVFCTKNNQEFWAYLGFKADTVLVDEDLQLISKSNTTIQLPLTIASDNNVL
                     LYPNPVKDNLFLQITEPLAQQLNIQIYNAFGSMVYSNLFKNISNNTLIKIPFSSYLKG
                     FYVVKIQTETGLKGIKKIIKY"
     gene            12620..>13849
                     /locus_tag="PLY81_00910"
     CDS             12620..>13849
                     /locus_tag="PLY81_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNF28819.1"
                     /translation="MSTYNQSRSRVIQIIFLLVFATIIGQLINLQIFSSKYRIMADNN
                     AIYRKVIYPDRGIIYDIKKRAILENTIMFDLMVIPSDTKGIDKVAFSNLLGIDTVEYR
                     KRITTAIIKNTSVKASVFEALLTPEMYARLNENIYKFPGFMLQERSIRSYPYNTAAAI
                     LGYIGEVDTSFLRKYKDEGYEMGDYTGLNGLERTYEKVLMGQRGIKRFIRDNKGRLQG
                     SFEKGLFDTVAIAGRNLYTSIDIEVQQLAEKLLQNKIGSAVAINPKTGSIIAMATSPS
                     YNPNSLTGSTRRKNFGKLLLDTARPLLNRAIKGQYPPGSTFKPLGGLVALDENIITPN
                     YGFPCGGAYYNCGKAVRCTHSGGGHASNLRNAIAYSCNSYFVQVFRMAVDNPLYANPQ
                     KGYLKWKEYMNNFGMGVK"
BASE COUNT         4682 a         2214 c         2023 g         4930 t
ORIGIN      
        1 aagggggggt aaactttgaa ccttataaag ctaaattatt tgaaagcatt ggaaagaaaa
       61 ttgatactat agaattattt cctgccagcg aaggtttttt tgcatttcaa aactcacagc
      121 aagaagaagg tttattgtta aacaccaact caggaatttt ttacgaattt attcctgctg
      181 cagaaatttt tactgaaaac ccaaccaggc tttgcttaca agatgttaag cttggagaaa
      241 attatgcact aatcattaat agcaatgcag gtttatgggg ttataatata ggcgatactg
      301 taaaatttgt tagtttaaat ccttataaaa ttattgtaag tggtagaata aaacatttta
      361 ttagtgcgtt tggagagcat gtaattgctg aagaagtaga acatgcatta ttaaaagcag
      421 ccaatgaaga aaatataaaa ataattgagt ttacagttgc cccaaatgta gcacaaggta
      481 aaggaaaaag ctttcatgaa tggtttatag agtttgaaaa tttgcccaaa aatttgccaa
      541 gctttataga gaaagtagat aagaatttac gagataaaaa tgtgtattac gacgatttaa
      601 taaaaggaaa tattttacaa accttgcata taaattgtat cagaaaaaat ggatttatag
      661 attatatgaa aagtatagga aaattaggag ggcaaaataa agtaccccgt ctaagtaatg
      721 atagaaaaat agcagaggaa ttaaatgggt ttataaataa ttaaacttgt tattactata
      781 aatattttca ttcagatgtt ttatcactat tactaatgat gttgtaatag aattaatttg
      841 cacatacatc tatttaaaaa cgaacctcat ttttgttaag attgaataaa caaaaggatt
      901 gtaaaaagtt atttagttat tgtttatttt atttaaaatt tgatgagcta cttccatagc
      961 taagtagcca tcaatttcat taacaatagt ttgtttattg tttaaaatgc tatctacaaa
     1021 agcttctaat tctaatttaa ttgcattagc ttctgttatt gtagggttag caactgcaat
     1081 agttttttta ccttttggcg tttctacatc aaaggcaaaa acattcttat ctgttggttc
     1141 tttaagttta attatttctg ttttcttttc taaaaaatca atacctatat aactatcttt
     1201 ttgaaacaaa cgcatttttc gcattttctt catgctaatt ctgctgctgg ttaagttagc
     1261 aacacagcca ttgttaaact ctattcttac gttagcaatg tctggtgtat ctgtcatcac
     1321 tgcaacacca tttgcactaa ttgttttcac atcgcttttt actaaactta aaataatgtc
     1381 taaatcgtgt atcattaaat ctagtattac gctaacttct gtacctcttg ggttaaattg
     1441 tgccaaccta tgtacttcaa taaacatagg gtttaacgat aaatctttta aggctaaata
     1501 agcagggtta aacctttcta catgccctac ttgcatttta atatttgctt ctctaaccat
     1561 ttccactaaa gctttagctt ctggaatagt gttgcataat ggcttttcaa caaaaacatg
     1621 tttgccttta cgtactgctt ttgaacaaac ttcaaaatgc cagtcggttg gtactacaat
     1681 atcaataata tcacaagcgt ctattaattt atcttcatct aaaaatcttt taatgccata
     1741 ggttgcagta acttctttag ctgcatcgtt gtttgggtca aaaaaaccaa ctattttaat
     1801 accttctatt tctttccagt tatttaaatg aaattttcct aaatggccta caccaaatac
     1861 acctacttta agcattatac attatttaag ataagactat gaatgatagt ttttaaaggg
     1921 gtaaagataa ataaacccta aaagtaaaaa cgttcaaaaa tgggtatgtg gaaatattgg
     1981 tattaaaatt atcaatatta ttttgcatgt tgaattgcta atgcaaattc taaaaggttt
     2041 ttaaatcttg catcaatagt tgcatggggg taaggtgttt gtgggtgtgt agtagcaaca
     2101 aaaacggtat gcatgcctgc atttttccca aattgcatat cacttaattt attgcctacc
     2161 ataatgcttt tagtaaaatc cattgcagga aaatcttttt ttgcctgcaa agccatacct
     2221 gtattgggtt ttcggcatgg cgaagtattt tccaaatctg aacaaaaata tattttatct
     2281 attctgccac cggcattttg tatttcttgc accatgtttt gatgaatgtt ttgtaaatct
     2341 ccctcactca ttaattgttt gccaatacct ctttgattgg taacaacaac aatagttgca
     2401 aaatattgct gaagtatttt caatgcttct tttacttctg ggtaaaaacg aaattcctcc
     2461 caattaaaaa tatagctatt ttctttttca tagttaatta ctccatctct gtctaaaaac
     2521 agagtccagt tattttttat attttttaat aattgcataa aaaaatacaa atgtgaaacg
     2581 caacaaatgc tgctacaaaa ttcaactgat ggttataaaa ctaaaataat ttttcttcca
     2641 ctaattcgca aataatatgc cccaacataa tatggctttc ttgaatacga ggtgtattat
     2701 tgcttggtac gtttaataaa tacttggtaa gcccttttat atcaccacca gtattacctg
     2761 taaatgctat ggttgtaata ttttttgcat ttgctacttc aaaagcctga ataatatttt
     2821 tactgttgcc actagttgtt aaacctatta aaatatctcc actattgcca attccatcta
     2881 gtagtcttgc ataaattgca tcgtaactat aatcatttgc aacagcagtt aagtaagatg
     2941 tgttgcagtg caaagcctct gctggcaatg ctttcctgtc tttataaaat cttccactaa
     3001 actcagcagc caaatgttgt gcatctgctg cactgccacc attgccacaa aacaacactt
     3061 tatttccgtt attaaaagcc tgtacaatac tattcacaca atcatcaatt gtttgaatga
     3121 gtgaattgtt attcaaaatt tcttgtttag ttgtaatgga agcttgtata attgatttta
     3181 ttttttgctg catgataatt tttagttttc gtttaatgaa cgctccatgt ttttaatcca
     3241 ttttttgtaa atgcataagg ttttactaca ccaccaaaac tatttaaagt ttctattact
     3301 ttataccttg tattttgtgg gcaataaaaa atcataaaac caccgcctcc agcaccactt
     3361 attttaccac ctgttgcacc tgcttttttt gctgcatcat aaatattttc aatattactg
     3421 ttagatatat tgtttgccat tttccttttt tgctgaaagc caaaatctaa tatttcgcca
     3481 aactcattta atttgccttt cagtaatgcc tctttcatca ttttggcttg ttcttttagc
     3541 tggtgcatag cttcaatact tttttcattt ttattcacta cgtttttttg ttgctctttt
     3601 attatagtag cactttctct acttgtagca gtaaaataca gtactaaatt attttctaat
     3661 tcgcttatat attctgaacg tattcttaat ggatttacaa ttactttatc gttattataa
     3721 aattccataa aattcacacc accaaaagta gctgcatatt gatcttgttt gcctcctgct
     3781 aaacttaaat catttctttc aatatcatag gcataatggg caatgtcata atctcctaaa
     3841 ggcaatttca gcatttctac aaaagcccca attatagcca ctactaaggt tgatgaagtg
     3901 cctaaaccac tgcctgctgg agcatcaaca aaagtgctga gtgtaaagtt ttgatgagca
     3961 aaaccataat ctttttgaat acgattataa acgcctttta ataaatctac agtgccatta
     4021 atgggtaatg ttttactaaa atcaaattgt tgaatttcat ttctgtctaa tgcctgaagt
     4081 gttataccac cagttttttc taattgaata gatgcataag catttaatga aatagttgca
     4141 tttaaaatgg caccaccaaa ttcatcgcta taagggctaa catctgtccc tcctcctgcc
     4201 aaacctatac gcaatggggc tttgctgcga taaatcataa ttgaaaatta ttataaatag
     4261 atgaattaag gttaatagct ttcggtttgg ttaggaaaat ttaaactttt aatatcagta
     4321 atatattgct gaactgcatt gaaagtaata tcgtgtaaag aggcatattg tctcaaaaaa
     4381 cgtggtttaa attctgtatt aattccaagc atatcatgca ttactaatac ttgtccatcg
     4441 caataagggc ctgcaccaat acctatagtt ggtatatcta tcgtttctga aacttgcttt
     4501 gctaattgtg caggtatttt ttctacaact actgcaaagc aaccagcctc ttgtaaaagt
     4561 tttgcatcgc ttaataattt atctgcttct gcttgttctt tggctctcac agtataggtt
     4621 ccaaatttat aaatactttg aggtgttaaa cccaaatgcc ccataacagg aataccggca
     4681 cctacaattt ttctaacact ttctacaact tctgcacctc cttcaagttt aactgaatgt
     4741 cctcctgttt ctttcataat tctaatggca gatgccaaag caatatcact attactttgg
     4801 taggtaccaa aaggtaaatc aaccacaacc aaacatctgt taattgctct tactacactt
     4861 tgtgcatgat atatcatttg gtctaaggtt attggcaaag ttgtttcatg accagccatt
     4921 acattgcttg cactatcgcc aaccaaaata atatctattc ctgcattgtc aataattttt
     4981 gcaaaggaaa aatcgtaagc agtaagcata gaaatttttt cgccagactg cttcattttt
     5041 tgtaatgtat tggtagtgat ttttttaacc tctttatgaa tggacattta aaatttttaa
     5101 gaagataagg aatagctttt aacgttgtaa aattaaagtt ttgtttctaa ttctaattat
     5161 tgaagaatag ttatttgata taaataccaa ttaacagtgc tattgcacat agaataaaaa
     5221 gaataactaa aaaaggtgtt ataaatttaa accaagtttt tgtactaata tttaccatgc
     5281 ttatagatgg taaaaataaa cccgttgggg taataaagcc cattattccc ataccgtata
     5341 aataactatt cacaatttct cttcctggta catgaactat taaagcaagt gcccctaaaa
     5401 ttggcatagt taaaacagcc ataccagatg atgatgaaat gaataaagtt aaaattatga
     5461 acaaccctaa caatattaaa ataaagaaag caggaggaaa actgccagta atttttgctg
     5521 tgtaaaataa caatgtatcg ctaatatttc catcatttaa aacaatagta attccacgtg
     5581 caatacctac aataaaagca acacccaaaa ggctttcggc tccttttata aattgttgaa
     5641 taaattcggc ttcctttgtt ttatttataa aaaataataa taaggatgct ccaaaaaata
     5701 atactgtaat ttcaagcaac caccatttta aaaatattac tcctgcaatc ataatgatga
     5761 atgcacttgc aaaaagtagt aataatagtt ttgttcttaa ggtaagtgtg ttgtttatat
     5821 cttcattatt tactgttgct gaataattat aatgtaggtt cccatcgtat tttaacacta
     5881 atgagtttgc aggatttgct tttatttttt tagcatatct taaaatatac caaattaata
     5941 aagcagtaga aataatgtac attaaaattc tttcgtaaat gccatcaatc cagtttaagc
     6001 ctgcagcatt agagccaatt atagtagcaa aaggattgct aaaagaagaa agtgtaccta
     6061 attgagaccc tgcaaaaatt acagcaacag gtattaataa atcgtaacca gcagctaaaa
     6121 ataaaggaac taatacagga taaaaaacta atgtttcttc tgccatacca taagaggagc
     6181 ctccaaatga aaacaaagct gtaagaataa ttattaaaaa ttgttctctt ccttttaatt
     6241 taaatgataa gtaggatatg ccttttagca tggcacctgt ttcgttaaat acataaataa
     6301 atccacccat tactaaaatt agcaatacaa tatctatagc atcgtaaatg ccttttatgg
     6361 gagaagataa taattgaata aaagttgttc tattgctttt tgtagcttga taagtgttag
     6421 gaattgagac aggttttgta atatctccat tcgtaaactt ttccaaagga ataatgatgt
     6481 gcaagctatc taaagtattt tgggttaaag gcacatgtgt attgatgcca ttactattaa
     6541 ttacaaaatt tttttccgat acagataatt tattgtacgt acctgctggt aaaagccaag
     6601 ttgcaattgc tgcaataatg gttactaaca acaaaattgt tattggatgc agcatctttt
     6661 tactagaaat catatataaa taattgaaat tgctttaaca atgttaaaat aaatataagt
     6721 atccatataa cttttattat aaatgagtgt ttaggaacca tagaaaataa gggagcattt
     6781 tttatagaaa ttttattttt cgattttctt tttattagaa attcagaaac cttacatttg
     6841 cagcggcagg tcttatacga ccagctcctg ctgaacctcc ccagggtagg aatacagcaa
     6901 ggataggcgg ttgagcggtg cgatgtgagt agcctgccac ttttttcttc taccaaattc
     6961 taaatatatt ttattagaat acggataatt tgcaaagaat aatttttcaa ttaatttaat
     7021 aaaaagctat gaaatttttt gttgatactg caaaccttaa tcaaataaaa gaagcaaatg
     7081 atttaggtat tttagatgga gttactacta acccttcttt aatggctaaa gagggtgtaa
     7141 aaggcgagga agctattatg aaccattata aaactatttg cgaaatggtt gatggtgata
     7201 ttagtgcaga agtgttgagt gttacattta atgaaattat tgaagaaggt aaaaaattag
     7261 ctgctattca tcctaacatt gttgttaaag taccaatgat aaaagatggt attaaagcaa
     7321 ttaaatggtt tacagataat ggtattaaaa caaactgcac tttaattttt agtgctgggc
     7381 aagctatact tgcagcaaaa gctggtgcta cttatatttc tcctttttta ggaagaattg
     7441 atgatagtgg ttgggatggt gttcaactaa tagaagaaat tgctcaaata tatgctgtac
     7501 aagggtttaa gagtgaaata ttggcagcat ctattcgtaa cccaaatcat attattaggt
     7561 gtgctgctgc tggtgcagat gtttgtactt gcccgttaga ttctatttta gggttattaa
     7621 aacatccttt aactgatatt ggcttagcca agtttttaga agatgctaaa aaaatgtaat
     7681 taatttctta ctataattta aaagctcaga taaattatct gagcttttta ttttgcttaa
     7741 ttaatttctt ttggagttag tttaatttgt agttcattta attgctttgt atctatacta
     7801 cttggtgcat caagcattac atctctacca ctattatttt taggaaaagc aataaagtct
     7861 ctaatacttt cgctaccccc taaaatagaa cataatctat caaaaccaaa agcaatacca
     7921 ccatgtggcg gagcaccaaa ttcaaaagct cctaataaaa aaccaaattt atgttgttgc
     7981 tcttcttcat tcatacctaa ggcagcaaac attttttgct gtaattcttt ttgataaatt
     8041 ctaatacttc ctccaccaat ttcattacca tttaaaacca tatcataagc attggcttta
     8101 atatttgcat acggatgttt taaataatct gcagcatttt ctatggctgg gttgttatga
     8161 atcattgttt caatatcact tggcttaggg cttgtaaaag gatgatgacg tgctacccat
     8221 ctattctctt cttcggcata ttcaaataaa ggaaaatcta caacccacaa tagtttaaac
     8281 tcatcctctt ttctataacc taatctctta cccatttcta atcttaattc gctaatagct
     8341 tttctggttc tttcctcttg gcctgcaagt attaaaatta aatctccggc ttttgcattt
     8401 gctgcttgtg caattgcttt taatttttct tcgctataaa atttatctac actgctttta
     8461 aatgtaccat cagtattaca cttaataaat accaaacctt tcattccaat ttgaggtcgt
     8521 tttacccatt cagttaattc gtctgtttgt ttgcgggtaa actcactaca atcaggaaca
     8581 gcaatggcta caacagtttc tgcatcatta aatactgcaa gatctgctgc aattaaatca
     8641 gaaatattgt ttttttctgg aaagctatga gcaggaaatt ttaagttgca tagctgcatg
     8701 ccaaatctaa tatctggttt atcatttcca tactgccaca ttgcttcttg ccaagtcatt
     8761 ctttcaacta cactggtata atcaatattt tttacttctt taaaaatact ttttatcaaa
     8821 ccctcaaaca tgttcaaaat atcttcttgt tcaacaaaac tcatttcaca atctatttgt
     8881 gtaaattctg gttgacggtc agctcttaaa tcttcatctc taaaacattt tacaatttga
     8941 tagtatttat cgtaaccact taccattaat aattgtttaa aagtttgtgg actttggggc
     9001 aaagcataaa actgccctac attcattcta cttggtacta caaaatctct tgcaccttct
     9061 ggcgtagatt ttattaagaa aggtgtttca atatctataa aaccattttc atgtaaataa
     9121 tttctggcac ttctgtttac tgcataacgt agttcaatat ttttctttac agcatttctt
     9181 ctcaagtcta aataacgata tttcattcta aggtcatcgc caccatccgt ttcatcttgt
     9241 atggtaaatg gtggtgtttc acttttgttt aaaatggtaa agttgtttac tgtaacttct
     9301 atttctccgg ttgcaatatt tttattttta ttacttcttt cagtaaccgt tccatttact
     9361 tgcaaaacaa attcacgtcc taaagaattt tcttctaatt ggttgttcag gttttcgcca
     9421 aaaagtattt gtgttatacc ataacggtct cttaaatcaa taaaagtaat tgcaccaaat
     9481 ttcctaattg tttgtaccca tcctgctagg gtaactattt caccaacgtt tttaattgtt
     9541 aattcaccac aagttctaga acgaaacata ctatttagat tattttaatt ttttgtaatc
     9601 aacggttgtt atataatgat acaattgttg cgtcgcaccc ttcaactttc gatgctttat
     9661 taggcaaagt ttgtaaggct tacagtattt gtacaacagt tgcaaatata tattaaataa
     9721 aaagagagta gcgtttaact accctctact tatatttttt ttaaatatta tctaattatt
     9781 ccaatttttt ccaaaaactc tttgtagtag tgggcttgtt ctagcttctg ctttagttct
     9841 aatatttacc tcttgctcag caattttatt aaacatagca ttggtaacaa caccagctaa
     9901 caaattatct aaatttaggt ttaagtttat tttattaccc aaaaatattt ttgctggtgc
     9961 aaccaattta tcccattctt tagtaacctg atattggtct aaggctgttt tcataattgg
    10021 cgtaacagtt gttcttaaat cattgccaat tgttgttcta aagtaatctg ttgcagcagt
    10081 tccaccattt ttaacaatat ttattgcatc ggttatattc attcttttta taccatctgc
    10141 aaaaacaggt atagagttag ttgctgtttg tgttgcagca ttggtaaggg tattggtaaa
    10201 tttatcaatt tctttactta ggcccatttg ctgcaatttt tgtaaaaccg tatttacatc
    10261 tttcggtaaa atagcattca ttaatacatc tttgctaatt aaagcatttc cattactgcc
    10321 aatagtaagc atttctttta tggcacttat agcatctctt tctgttaaaa tactatctgc
    10381 tacacttttt aatgtgtcgc aactactaaa aaacatacta caactaacca ataaggctaa
    10441 ataaactctt ctcataacat aataaaatta aggggtcaaa cataagattt ataactaaca
    10501 tattgtaata aagtattggt gataatttaa taaaatactt ttattcaatt aattaatttt
    10561 atgcaatgat taggctttta ctatttatta gtatcttctt ttataaagtt aatgcacaag
    10621 agatggagaa cagaacagtt tccaatcttt ctttaaccaa tactatacta acagctccaa
    10681 aattcaatac accagctatt tcaaaagctt ctaacaattt tcacattcat tacttaaagt
    10741 ttaaattaaa agtaaaccca gcaataagat atattgatgg cagtgtagat atatatttta
    10801 ttccaaacat actaaccgat agtatagagt tagatttatc ctatcagtta aaggtagata
    10861 gtatagtatt taataatcaa aaattaagtt acatacaaca cccaaataat actttaatta
    10921 tttacttcca aacagcctta gttgctgcac aaaactattc catgaatata ttttataaag
    10981 gggtgcctgg cgatagtggc tatggttctt ttatacaaac tacacataat aatacacctg
    11041 ttatttggac attaagcgaa ccttttggaa gtaaagattg gtttccttgt aaaaatgaat
    11101 taagcaataa aatagatagt gtagatatgt ttattacgca tccttctgtt tataaagcaa
    11161 cagcaaatgg tgtgttgcaa tatgagcaaa ctaatggtgg taatacaaca acctttttta
    11221 aacaccgtta tccaattgct acttatttaa ttgcttttgc tgtaagtaac tattcggttc
    11281 aacaaataaa aatgaagatt ggaattgaca ccctacaata tgtatcttat atctatcctg
    11341 agtcggttgc tgattttcaa aatttaatca ttcctactat aaatgctctg caacttttta
    11401 gcaatacatt tggcacttat ccttttatta aagaacaata tggcataaca caatttgggt
    11461 ggggtggtgg tatggagcat caaacaaata gttttttatc ggcacccgat accaatttaa
    11521 tggttcatga attagcacac caatggtttg gtaacaaagt tacttgccat agttggcagc
    11581 atatttggtt aaatgaagga ttggcaacat ttgcagctaa ttactatttt gaaaaagtag
    11641 attctgcaaa acttttatca tttttaacta cacaactaaa taatattact aagttgccag
    11701 atggtaaaat tattgtatca gacacattta atgttggtag tatttttaac tctaggttaa
    11761 gctacgacaa agcagctttt atgttaagaa tgttaagata tacattaggc gatagtattt
    11821 tttttaaagc tgttttcaat tatataaatg acccattatt gcaatacaat tttgtaacca
    11881 ctaatcattt tatacaacat gtagaggaag tagcaaagca agatttaagt tggtttttta
    11941 atcaatggct taatggtgaa ggctttccaa cttttaatgt ttcttggagt aataataatt
    12001 ttaactgggt taaagttatt gttaaccaaa caacttcaca ttcatcggtt cctttctaca
    12061 aaactgtatt gccattaaca tttaaaaaca atcatcaaca aaaaacaata aaagtatttt
    12121 gcacaaaaaa taatcaagag ttttgggcct atttaggttt taaagcagat accgttttag
    12181 tagatgaaga tttacaatta attagtaaaa gcaatacaac cattcaatta cccttaacta
    12241 ttgcatcaga taacaatgtg cttttatatc ctaaccctgt taaagataat ttgtttctac
    12301 aaattacaga gccattagca cagcaattaa atattcaaat ttataatgct tttggttcta
    12361 tggtttactc caatttgttt aaaaatatta gtaataatac attaattaaa attccattta
    12421 gctcttattt aaaagggttt tacgtggtta aaatacaaac cgaaaccggt ttgaagggta
    12481 taaaaaaaat aattaaatat taataatttg cttgttaaca tttatgaata actttttatg
    12541 tgtacattat tgttactacc aaatacattc gtcaaatttg ctagtattgt ttattaaaaa
    12601 ttgtattatt taagctacca tgtccaccta caatcaaagc agaagcaggg ttattcaaat
    12661 aattttttta ttagtatttg ctactattat tggacaacta atcaatcttc aaattttttc
    12721 ttctaaatac agaataatgg cagataataa tgccatatat agaaaagtaa tttacccaga
    12781 taggggaatt atttatgaca taaaaaaaag agcaattcta gaaaacacca ttatgtttga
    12841 tttaatggtt attccatcgg atacaaaagg tatagataaa gtggctttta gcaatttatt
    12901 aggaatagat actgtagagt atagaaaaag aattacaaca gcaattataa aaaacacatc
    12961 ggtaaaagca agtgtatttg aagctttatt aacaccagaa atgtatgcaa gattaaatga
    13021 aaacatttat aaatttcctg ggtttatgct tcaagaacga agtattagaa gctatccata
    13081 taatactgct gctgccattt taggatatat aggtgaagta gatacctctt tcttaagaaa
    13141 atataaagac gaaggttatg aaatgggaga ttatacagga ttgaatggtt tagaaagaac
    13201 ttatgaaaaa gtactgatgg ggcaaagagg tataaaacgt tttataagag ataataaagg
    13261 aagattacaa ggcagttttg aaaaaggatt gtttgataca gttgctattg caggtagaaa
    13321 tctttatacc agtattgata tagaagtgca acaattagcc gaaaaattgt tacaaaataa
    13381 aattggcagt gcagttgcca taaaccctaa aactggtagc ataattgcaa tggcaacaag
    13441 tccaagttac aatcctaatt ctttaacagg ttcaacaaga agaaaaaatt ttggaaaact
    13501 tttattagat actgccaggc cattattaaa cagggcaatt aaagggcaat atcctccagg
    13561 ctcaacattt aaaccattag gtggattagt agctttagat gaaaacatta ttactccaaa
    13621 ctatggtttc ccatgtggtg gtgcatatta taattgtggt aaagcagttc gttgtacaca
    13681 tagtggtgga ggacatgctt ctaatttaag aaatgctatt gcttattctt gcaattcata
    13741 ctttgtgcaa gtgtttagaa tggctgtgga taatccttta tatgctaacc ctcaaaaagg
    13801 gtacttaaaa tggaaagaat atatgaacaa ctttggaatg ggtgtaaag
//
LOCUS       DAOQHP010000013        13548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2194_length_13548_cov_4.114430, whole genome shotgun sequence.
ACCESSION   DAOQHP010000013 DAOQHP010000000
VERSION     DAOQHP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13548
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_13548_cov_4.114430"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLY81_00915"
     CDS             <1..343
                     /locus_tag="PLY81_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNF28820.1"
                     /translation="LLHVVDIAHPQYEDQIGTVNKTLQDLKAFDKPVLTIFNKMDLYE
                     KRYFDEWLHSEVKQDILLELKEKWVNNTDNNCVFISALERKNIDELRAVILNKVRDIY
                     QIRYPYKTIHY"
     gene            350..700
                     /locus_tag="PLY81_00920"
     CDS             350..700
                     /locus_tag="PLY81_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNF28821.1"
                     /translation="MQDIKWYKIADSINLLPWQANNMCLVEVKNKIYTIAKHNNKIFA
                     FSHKCPHASGILANGWIDALANVVCPIHRYKFCMQNGRNTSGEGYFLKTYKVEIREEG
                     VFIAIENKGFLSFF"
     gene            802..875
                     /locus_tag="PLY81_00925"
     tRNA            802..875
                     /locus_tag="PLY81_00925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:836..838,aa:Asn,seq:gtt)
     gene            complement(1012..1404)
                     /locus_tag="PLY81_00930"
     CDS             complement(1012..1404)
                     /locus_tag="PLY81_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28822.1"
                     /translation="MEHSIVKKCLSCGKVVKGRTDKKFCDDYCRNNFNNQKKSKENYS
                     PYVKGILNTLLKNRKILETLLPPDKQTIHFKKEKLVQAGFIFKYFTHTYINKQRETYF
                     YCFDFGYKQLENDWLMIVKRREGDERSI"
     gene            1704..2051
                     /gene="ssb"
                     /locus_tag="PLY81_00935"
     CDS             1704..2051
                     /gene="ssb"
                     /locus_tag="PLY81_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF28823.1"
                     /translation="MYALKNKVQLIGNLGNNPEVKTLETGKKMARFSMATKEVFRNAK
                     GEKVVETQWHNIVAFGKVADIVEKYLQKGTEMAMEGKLINRSYIDKEGNKKYITEVQV
                     NEVLLLGKENAKA"
     gene            2219..3106
                     /locus_tag="PLY81_00940"
     CDS             2219..3106
                     /locus_tag="PLY81_00940"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNF28824.1"
                     /translation="MAFNIQTFKTRTLSAIVFAAIMLFGLFVNNWTFFLLFTIIHFGC
                     WIEYQKLLSLIDDCYKTITPFHKYGVIITGFGFMLWMLNASYAFGSFNLQLLGMSLVV
                     IFAIVLPITEILFSKKQEVKPAFLSFLGLIYISMSIGLILDLYEININNAIIGNGIIA
                     IFIIVCMWVNDTMAYIVGSFIGKTPFSKISPKKTWEGTIGGIVLCIIVMGFAMNYFIE
                     EKQLIGQSASLKVHWFILAAIASITGTLGDLLESKLKRMAGVKDSGNIMPGHGGFLDR
                     FDSLLVAVPFIWLYVKLFL"
     gene            3140..3757
                     /gene="recR"
                     /locus_tag="PLY81_00945"
     CDS             3140..3757
                     /gene="recR"
                     /locus_tag="PLY81_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNF28825.1"
                     /translation="MQLPSVLLENAVAEFAKLPGIGKKTALRLVLHLLNKNEEEVKLF
                     GDTIYTMRNSIKFCNRCFNISDGDICSICSNSMRNQQIICVVENIRDVIAIESTQQYN
                     GVYHVLGGVISPLDGIGPDKLNINGLLSRVENDQVEEIVFALSPNIQGDTTIYYIQKK
                     LQNTNCKITTIARGIAFGGELEYADEMTLAGSLQNRLPVQQYVNG"
     gene            complement(3759..5018)
                     /gene="chrA"
                     /locus_tag="PLY81_00950"
     CDS             complement(3759..5018)
                     /gene="chrA"
                     /locus_tag="PLY81_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNF28826.1"
                     /translation="MILLRHIPFLKAVFLYSITAFGGPQGHYGMMLKTFVKQRKYVSE
                     KELLDLVSFCNMIPGASSTQTITLIGYKRGGTGLALLTLLIWIFPASVLMGGLSFLLS
                     YLDKNSINTQWFRFIQPMAVGFIAFSSLKLYKVCINNTITRIILVAASFITFFAFKSP
                     WVFPVLIICAGIATNFSDKRIPQKTLPTQKIKWANILIFLFLFGLTGYISETATRQNW
                     ENRKAINLFENNYRFGSLVFGGGDVLMPMMYEQYVVRPKTEKILKSKRDVLKMSKEEF
                     LTGSGMVRAIPGPVFSIASFTGGMLLRNEGSPLLHVLGCVLGVIGIFLPSALLVLFFF
                     PIWHNLKKYAVIFRSLEGINAVVVGIMAGAFFYLAKDILFTDVQISLKNFSLDVVVII
                     ATFLTLEYTKTKAPFIVFACLLLGWLL"
     gene            5148..6272
                     /gene="mqnC"
                     /locus_tag="PLY81_00955"
     CDS             5148..6272
                     /gene="mqnC"
                     /locus_tag="PLY81_00955"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNF28827.1"
                     /translation="MQLSNLYNKALQFEFLSIEEGMFLFKHAPLTELMHIANEMKKHH
                     VPHGKVTWQIDRNLNTTNVCIANCKFCNFYRIPGHSEAYITDMPTYRKKIQETINYGG
                     DQLLLQGGHHPNLGLDFYTKIFREIKQEFPTIKLHALGPPEVAHITKLEKSTHYEVLK
                     ALQNAGLDSLPGAGAEILVDRVRRLVSKGKCGADEWLEIMHQAHKLNITTSATMMFGH
                     VETVEERFIHLSKIREIQAKKPDSAKGFLAFIPWTFQDVDTLLAKIRGVHNLTTSEEY
                     IRTIAISRIMLPNIKNIQASWLTVGKETAQICLHGGANDFGSIMLEENVVSAAGAPHR
                     FTYKTIQNAISEAGFGPQLRNQQYEWRDLPNFIQEQVINY"
     gene            6362..6847
                     /locus_tag="PLY81_00960"
     CDS             6362..6847
                     /locus_tag="PLY81_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF28828.1"
                     /translation="MTERDYNNCVNQYADNVYRFILKNIKQEEDAKDIVQTAFEKLWR
                     NKENVETDKSKSYLFTIAYNQMIDHIRKNKRIVLTESFKDNVRINHPTDSFSKNVFDE
                     ALSKLNDIQKSLVMLKDYEGYSYEEIGKITNLNASQVKVYLHRARIILRDFLVKRENV
                     L"
     gene            6871..7977
                     /locus_tag="PLY81_00965"
     CDS             6871..7977
                     /locus_tag="PLY81_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28829.1"
                     /translation="MKMEINRNNYQEYFLLYLDDELSVHQKNMVDEFLLIHKDLKVEF
                     NLFKQTKLPNPTLISANKSTLYRFDIDTIHKANYEENFLLYADNELDENKRQAVEKFV
                     LQNPSYQNEFTVLKMATLQPENIQCPFKNELLKNERKPIIYFGFKPVAIAASLVLIAV
                     AVWLLNFNNTIINNNNIAFNSQTNNSQNNNTSSINNLQAKTSIPTISNNKVQPSTLYN
                     QQEEPKVQSTSNTNLATTIINQNNEVNNAVTNSTVSTIHNTTKQEQNIVEVDLKSVYQ
                     NANYKTLQTSNNTALNTLIQVNNNIEETNMVQQSTFQYIDINEITENESSNEKLINIG
                     NIKVKKSKLKNIFKKAKKIIGAPIDNKAIALATL"
     gene            8009..8860
                     /locus_tag="PLY81_00970"
     CDS             8009..8860
                     /locus_tag="PLY81_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF28830.1"
                     /translation="MKKTILTAVLLSAAYITFAQTESKENTEDTVKAGNFFIVKKNGK
                     TGNKESKGFKISIHNSEDKNKNISTNWFVLDLGFANYRDETNYTNAQAGSYLRTLYTP
                     AGAVTSQSLRLKTGKSSNVNLWFFMQRLNISQHVFNLKYGLGLEMYNFRFDRNVSYRK
                     DPNPFIFNDSINFSKNKLYAGYITLPLMVNINTKPNSKKSFSFSAGVSAGYLISTRNK
                     QVSDTRGKIKYKGDFDLQPFRLAAVGEVGVGPVRLYASYSLNALHNKATLLNQYPYAI
                     GLRLSSW"
     gene            complement(8930..9172)
                     /locus_tag="PLY81_00975"
     CDS             complement(8930..9172)
                     /locus_tag="PLY81_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28831.1"
                     /translation="MKKFLMLAASVILFVGVALACGDKKDCKKGKDCCQKEAKAKNKE
                     ACSKESKDKVCCKKDGAEKKEVKKEEKKETKTEKKA"
     gene            complement(9335..9991)
                     /locus_tag="PLY81_00980"
     CDS             complement(9335..9991)
                     /locus_tag="PLY81_00980"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNF28832.1"
                     /translation="MTIHREGYQTIGITALFFGVFNVVSFYFFSASMPWLALLVFFIT
                     LFFLLFIISFFRIPNRKLTIDENKIICPADGKVVVIEETTDVEYFNDKRIQVSIFMSP
                     ANVHVNRNPMSGEVLYNKYHKGKYLVAWHPKSSTENERWSVVVKNNFGTILYKQIAGA
                     LAKRICNYTQVGQQVNQCKEYGFIKFGSRVDILLPIGTKINVQLNQKVQGGVTVLAEW
                     "
     gene            10304..11953
                     /locus_tag="PLY81_00985"
     CDS             10304..11953
                     /locus_tag="PLY81_00985"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNF28833.1"
                     /translation="MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGFKVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIFTSQSNNVTTGRIYQTVINKE
                     RAGEFLGKTVQVVPHITDEIKRRMQLIGKTGEYDIVITELGGTVGDIESLPFIEAVRQ
                     LQWELPDEDVVVVHLTLVPYLKAAKELKTKPTQHSVKMLSETGVHPDVIVCRTEEPLG
                     NDIKRKIALFCNVKQEAVIEAMDASTIYEVPLLMLREKLDLFCLKKLNIKQFNDPQLD
                     KWKAFLDKLKYPKAKITIGLIGKYVELQDAYKSILESFIHAGAINEVKIQVVNIHSEF
                     ITKDNVAEKLEGLDGLLVAPGFGSRGIDGKIFAVQFARENSLPFFGICLGMQMAVIEY
                     GRNILGLNDAHSVEMEPTTVSPVINMMEEQKKIKMMGGTMRLGAYPCKLQEKSLAYKI
                     YGNSQISERHRHRYEFNNDYLEMYEQAGMIASGKNEETGLVEIIEIPSHPFFIGVQFH
                     PELKSTVEEPAPLFVHFVGAAKKYNETKSALISSLLQRETI"
     gene            12059..>13548
                     /locus_tag="PLY81_00990"
     CDS             12059..>13548
                     /locus_tag="PLY81_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNF28834.1"
                     /translation="MEDRFLLLLILIMVMIGIVMLAKKIRVAYPILLVVAGLLISFIP
                     GMPTIELRPELIFILVLPPILYEAAWACSFRELFKWRRIILSFAFFGVFITAISVAFT
                     AYWFIPGFTLALGFLLGGIVSPPDAVSTNAITKFVKLPKRISTILEGESLLNDASSLI
                     IIQFAILAVVTGNFNASKASLQFAWMVIGGVACGLLFGWIFVKAHKKLPTDANMDVLL
                     TLIAPYLMYISAEEIHTSGVLSVVAGGLFVSQKRYDFLSSTSRVRGTNVWQSFVFLLN
                     GIVFILIGLDLPQITAGLEGTNITTAIGYGILITIVVILVRLLSFYGAVGVTMIMRNF
                     ITVADRQNPGFKGPFILGWAGMRGVVSLAAALSIPFYLDNGEVFPQRNLILFITFIVI
                     LLTLVIQGLTLPFLIKRAKLKEIDYPQPEDEVSAYLDKELLKISIDYLRSVFPEKEQK
                     SKRFHEILVNVQEQLSENNEIKLDSKSTHIYCELLTLQRKQLLQIN"
BASE COUNT         4972 a         1920 c         2142 g         4514 t
ORIGIN      
        1 tttattacat gtggtagata ttgctcaccc acaatatgaa gatcaaattg gcacagtaaa
       61 taaaacctta caagatttaa aagcttttga taaacctgta ttaaccattt ttaataaaat
      121 ggatttgtat gaaaaaagat attttgatga atggttgcat agcgaagtaa aacaagatat
      181 tttattggaa ttaaaagaaa aatgggtaaa caataccgat aataattgtg tatttatttc
      241 ggcattagaa agaaaaaata tagatgaact tagagcagtt attttaaata aagtaagaga
      301 tatttatcaa attagatatc cgtataaaac tattcattat taaaagaata tgcaagatat
      361 aaaatggtat aaaattgcag attcaattaa cttgttacca tggcaagcaa ataatatgtg
      421 tttggtggag gtaaaaaata aaatatatac aattgctaaa cataacaata aaatttttgc
      481 cttttcgcac aaatgccctc atgcaagtgg cattttagca aatggttgga tagatgcttt
      541 ggctaatgtt gtttgcccaa tacatagata taaattttgt atgcaaaatg gtagaaatac
      601 gagtggcgaa gggtattttt taaaaaccta taaagtagaa ataagagaag aaggtgtatt
      661 tattgccata gaaaacaaag gttttctctc atttttttaa atagcggcaa aaaaattttg
      721 ctttcaaata ctacaacctt atttttgctg ccccgaaagg gaaataatat ccaaaatagt
      781 tcttatctta catcaaaata ttcttcctta gctcagttgg ttagagcatc tgactgttaa
      841 tcagagggtc gttggttcaa gtccaacagg gagagcaaag gttggtattg tttataccaa
      901 ccttttttat ataatattgc tacctaatat cttctttaag aacaattaaa atacaaatac
      961 agagatattg tttatatttt gctgcttatt attggttttt tgtaactgta tttatatact
     1021 tctttcatct ccttctcttc tttttacaat catcagccaa tcattttcta gttgtttata
     1081 accaaaatca aagcaataaa aataggtctc acgttgtttg ttaatgtaag tatgagtaaa
     1141 atatttaaaa ataaaacctg cttgtaccag tttttctttt ttaaaatgga tagtttgttt
     1201 atctggtggc aaaagggtct ctaaaatttt tctgtttttt aataatgtat ttaaaattcc
     1261 ttttacatag gggctataat tctctttact ttttttctgg ttattaaaat tatttctaca
     1321 ataatcatcg cagaactttt tatcggttct gccttttaca actttaccac aagaaaggca
     1381 tttttttact attgaatgct ccataaaaaa taatttatta tctaaaaata attttcaatt
     1441 ttctgctttc tattttttat taaatacttt gatttacaac taaaatgtac aattaaatat
     1501 tcgtgtatag tcgtttaaaa acgcttaaca tcgagagtaa acacttaaaa accgagtctt
     1561 gttttttacc ctaatcatct ttgccctatc aaataaaaaa gtatcagacg tcaatactac
     1621 tttgtttgat tgaaataaat taataaacca ttcttttttt caaaaccaat aaaaatgaaa
     1681 ttaagaataa aaatcttaaa attatgtacg cactaaaaaa caaagttcaa ctaattggca
     1741 acttaggcaa taatccagaa gtaaaaacat tagaaactgg taaaaaaatg gctcgtttta
     1801 gcatggccac caaagaagtt tttagaaatg caaaaggcga aaaagtagta gaaacccaat
     1861 ggcataatat tgtagctttt ggtaaagtag ccgatattgt tgagaagtat ttacaaaaag
     1921 gaactgaaat ggcaatggaa ggtaaactaa ttaataggtc ttatattgat aaagaaggaa
     1981 ataaaaaata cattaccgaa gtacaagtaa atgaagtatt attattagga aaagaaaatg
     2041 ctaaagcata aatagaaact atagtaaaca taataaaaat gtgtactact acggatgtaa
     2101 caaggttgtt aaactaactt tgttgcatcc ttttctttta tgtataatgc aaaggattaa
     2161 aatatttaaa ctgatattaa attttttatg caaattcgta cagtagtttc caacaattat
     2221 ggcttttaat attcaaacat ttaaaacacg tacactcagt gcaattgttt ttgctgcaat
     2281 aatgttgttt gggttatttg taaacaactg gacatttttt ttactcttta ccattataca
     2341 ttttgggtgt tggatagaat atcaaaaact attatcgctt atagatgatt gttataaaac
     2401 aattacacct tttcacaaat atggtgtaat aattacgggc tttggtttta tgctttggat
     2461 gttaaatgca agttatgctt ttgggtcatt taacctgcaa ttattaggaa tgagtttggt
     2521 agttattttt gctattgtac ttcctataac cgaaatttta ttcagtaaaa aacaagaagt
     2581 taaacctgcc tttttatcgt ttttgggctt gatttatatt tccatgagca ttgggttaat
     2641 tttagacttg tacgagataa atattaataa cgctataata ggcaatggta ttattgcaat
     2701 ttttattata gtatgtatgt gggtaaacga tacaatggca tatatagttg gctcatttat
     2761 tggtaaaaca cctttctcta aaatttctcc taaaaaaact tgggagggta ccattggcgg
     2821 aattgtattg tgtataatag ttatgggttt tgcaatgaat tattttatag aagaaaaaca
     2881 acttattggc caaagtgcta gcttaaaagt acattggttt atattggcag caattgcatc
     2941 tattacaggt actttaggag atttactgga aagcaaactt aaaagaatgg ctggtgtaaa
     3001 agatagtgga aacattatgc caggccatgg cggtttttta gatagatttg attcattgct
     3061 agtagcagta ccatttattt ggttgtatgt aaaattattt ttataaaggc taacaattag
     3121 cagttattat ctttgtatta tgcaattacc ttctgtttta ttagaaaatg ctgtagctga
     3181 gtttgcaaaa ctaccaggta ttggtaaaaa aaccgcttta agattagtgc tccatttatt
     3241 aaataaaaat gaagaagaag taaagttatt tggcgataca atttatacaa tgcgtaatag
     3301 tataaaattt tgtaatcgtt gctttaatat tagcgatgga gacatttgta gtatttgcag
     3361 taatagcatg cgtaatcaac aaattatttg tgtagttgaa aatattagag atgtaattgc
     3421 aattgaaagc actcaacaat acaatggtgt gtatcatgtt ttaggaggtg ttatttctcc
     3481 attagatggt attggaccag ataaattaaa tattaatggt ttactttcaa gagtagaaaa
     3541 tgaccaagta gaggaaattg tttttgccct atcacccaat attcaaggag atacaactat
     3601 ctattatatt cagaaaaaat tacaaaatac taattgtaaa attaccacta ttgctcgtgg
     3661 tattgctttt ggaggagagt tggaatatgc cgatgaaatg acattagctg gaagtttaca
     3721 aaataggctt cctgtacagc agtatgttaa tggataaact acagtaacca tcctaaaagc
     3781 aaacaggcaa atacaataaa aggagctttg gtttttgtat attcaagagt taaaaaagta
     3841 gctataatta ctacaacatc taagctaaaa ttctttaacg aaatttgtac atcggtaaat
     3901 aaaatatctt ttgctaaata aaaaaatgca cctgccataa ttcctacaac cactgcatta
     3961 ataccctcta acgatctaaa aattacagca tattttttta agttatgcca tataggaaaa
     4021 aagaataaaa ccaataaagc actaggcaaa aaaataccta ttacacccag cacacaacct
     4081 aaaacatgaa gtaatgggct accttcattt cttaacaaca taccacctgt aaaagaggca
     4141 atactaaaaa ctggccctgg tatagctctt accatcccac ttcctgttaa aaattcttct
     4201 ttactcattt ttaaaacatc tcttttactt ttcaatattt tctctgtctt tggtctaaca
     4261 acatactgct catacatcat aggcatcaaa acatcgccac cgccaaacac taagcttcca
     4321 aacctgtaat tgttttcaaa taggttaatg gcttttctgt tttcccaatt ttgcctggtt
     4381 gcagtttccg atatataccc agttaaacca aatagaaaaa gaaaaattaa aatatttgcc
     4441 cactttattt tttgtgtagg taatgttttt tgaggtattc ttttatcgct aaaatttgtt
     4501 gctattcctg cacaaataat taaaacagga aaaacccaag gcgatttaaa agcaaaaaat
     4561 gttataaatg atgcagcaac taaaataatt cttgtaatgg tgttattaat acaaacctta
     4621 taaagcttta atgatgaaaa tgctataaac ccaactgcca ttggttgaat aaacctgaac
     4681 cattgagtgt taattgaatt tttgtctaaa taacttaata aaaaagacaa accacccatt
     4741 aaaacgcttg caggaaaaat ccaaattaat aaagttaata aagccaaacc tgttccacca
     4801 cgtttataac ctattaatgt aatagtttgt gtagatgatg caccaggaat catattacaa
     4861 aaactaacta aatctaaaag ctctttttcc gatacgtatt ttctttgttt tacaaaagtt
     4921 ttaagcatca ttccataatg cccttgtggt cctccaaagg cagtaatgct atataaaaaa
     4981 acagctttta aaaatggtat atgtcttaat aaaatcaaaa gcagtatttt tttttaaact
     5041 agttacaaga taaagaaaaa taaaatgtaa caatattcaa tttcaataac taacccattt
     5101 attgcaaagc ctatacacct taactttgct aacttataaa taccataatg caattatcaa
     5161 atttatataa caaagcgtta caatttgaat ttttaagtat agaagaaggt atgtttttgt
     5221 ttaaacatgc acctttaaca gagttgatgc atatagcaaa tgaaatgaaa aaacaccatg
     5281 tgccacatgg taaagtaact tggcaaattg atagaaactt aaacacaact aatgtatgta
     5341 ttgccaattg taaattttgt aatttttata gaatacctgg ccattcagaa gcatatatta
     5401 ccgatatgcc cacttataga aaaaaaattc aggaaaccat taattatgga ggagatcaac
     5461 tattactgca aggagggcat cacccaaact taggcttaga tttttataca aaaatattta
     5521 gagagataaa gcaagaattt cctaccatta aattacatgc tttgggcccc cctgaggttg
     5581 cacatattac taagctggaa aaaagtaccc actacgaagt tttaaaagct ttgcaaaatg
     5641 caggtttaga tagtttgcca ggtgcaggtg ccgaaatttt agtagataga gtgagaagat
     5701 tagtaagtaa aggaaaatgt ggggcagatg aatggctgga aattatgcat caagcccaca
     5761 agctaaatat tactaccagt gcaactatga tgtttggaca tgtagaaaca gtagaagaaa
     5821 gatttattca tttaagtaaa ataagagaaa tacaagctaa gaaacccgat tctgcaaaag
     5881 gttttttagc atttatacca tggacttttc aagatgtaga taccttgtta gctaaaatta
     5941 gaggagtgca taatctcact acttccgaag aatatattag aactattgca attagccgta
     6001 taatgttacc caatattaaa aatattcagg caagttggct aactgttgga aaagaaactg
     6061 cacaaatatg tttgcatggc ggtgcaaacg attttggaag tataatgtta gaagaaaatg
     6121 tagtaagtgc agccggtgcc cctcatagat ttacttataa aacaattcaa aatgccattt
     6181 ccgaagctgg ttttggacct caattacgca accaacaata tgaatggaga gatttgccaa
     6241 actttataca agaacaggtt ataaattatt aaaattaatt tttaccaagt gtaacattat
     6301 tgtacctttt gcgtattact tacattaatt agaacttaaa caaaactacc ttagctgttg
     6361 catgacagaa agagattaca ataattgtgt aaaccaatat gcagataatg tgtataggtt
     6421 tattttaaaa aatattaagc aagaggaaga tgctaaggat attgtgcaaa ctgcatttga
     6481 aaaactatgg agaaacaagg aaaatgttga aactgataaa tcaaaatcat acttgtttac
     6541 tatagcttac aatcaaatga tagaccatat tagaaaaaat aaaagaattg ttttaaccga
     6601 aagttttaaa gacaatgtaa gaataaatca cccaaccgat agcttttcta aaaatgtttt
     6661 tgatgaagct ttatcaaaat taaacgatat acaaaaaagt ttagttatgt taaaagatta
     6721 tgaaggatat agctacgagg aaattggaaa aattactaac ctaaatgcca gtcaggtaaa
     6781 agtttatttg catagagcaa gaattatact aagagatttt ttagtaaaaa gagaaaatgt
     6841 gttataagtt tttagtagaa aaatgaagca atgaaaatgg aaattaatag aaacaattat
     6901 caagaatatt ttttacttta tttagatgat gagctatctg ttcatcaaaa aaatatggta
     6961 gatgaatttt tattaataca caaagatcta aaagtagagt ttaatttatt taaacaaact
     7021 aaattaccta acccaacctt aataagtgct aataaaagca ctttgtacag atttgatata
     7081 gataccatcc ataaagctaa ctatgaagaa aattttttat tatatgccga taatgaatta
     7141 gatgaaaata aaaggcaagc agttgaaaaa tttgttttgc aaaatccaag ttatcaaaac
     7201 gaatttactg tattaaaaat ggcaactctt caaccggaaa atattcaatg cccttttaaa
     7261 aatgagttac ttaaaaatga aagaaagcca attatctatt ttggatttaa acctgttgct
     7321 atagctgctt ctttagtatt aatagctgta gctgtttggc tattaaattt taataataca
     7381 attatcaata acaataatat tgcttttaac agccaaacta ataattctca aaacaataac
     7441 acctcttcaa taaataactt acaagcaaaa acaagcatac ctactatatc taataataaa
     7501 gttcagcctt ctacactata taaccaacaa gaggagccta aggtgcaaag cacttctaat
     7561 actaatcttg caacaacaat tattaatcaa aataatgagg taaataatgc agtaactaat
     7621 tcaacagttt caaccatcca caacactacc aaacaagaac aaaacatagt tgaagttgac
     7681 ttaaagagtg tatatcagaa tgcaaattat aaaacactac aaacttctaa taatacagct
     7741 ttaaatacat taattcaagt taacaataat atagaggaaa ccaatatggt acaacaaagt
     7801 acatttcaat atatagatat aaatgagatt acagaaaacg aaagcagcaa tgaaaaacta
     7861 attaatattg gtaacataaa agttaaaaaa agtaagctaa aaaatatttt taaaaaagca
     7921 aagaaaataa ttggtgcacc tatagataat aaggcaatag ccttagccac tttataacta
     7981 tttacaaaat tttatactaa atttcattat gaaaaaaact atacttacag cagttctctt
     8041 aagtgctgca tacatcacat ttgctcaaac agaaagtaaa gaaaacacag aagatacagt
     8101 taaggcagga aattttttta ttgtaaaaaa gaatggcaaa acaggcaata aagaaagcaa
     8161 agggtttaaa atatccatcc ataatagtga agataaaaac aaaaatattt ctaccaattg
     8221 gtttgtatta gatttaggct ttgctaatta tagagatgaa actaattata ccaatgcaca
     8281 ggcaggtagt tatttaagaa ctttatacac tccagcaggt gcagtaacca gtcaaagttt
     8341 aagacttaaa actggcaaaa gctctaacgt aaacctttgg ttttttatgc aaaggttaaa
     8401 tattagccag catgtattta atttaaagta tggcttaggt ttagagatgt acaattttag
     8461 gtttgacaga aatgttagtt atagaaaaga tcctaatcct tttattttta atgactccat
     8521 caatttttca aaaaacaaat tatacgcagg ctatattaca ttacccttaa tggttaatat
     8581 taataccaag cccaattcta aaaaaagttt tagttttagt gcaggagtaa gtgcaggata
     8641 tttaattagt acaagaaaca aacaagtaag cgataccaga ggaaaaataa aatacaaagg
     8701 cgattttgat ttacaacctt ttcgtttagc tgctgttggc gaagttggtg taggacctgt
     8761 aagactttat gccagttata gtttaaatgc tttgcataat aaagcaacat tattaaatca
     8821 gtacccttat gcaattgggt taagattaag cagttggtaa tgttcagttt taattaaaaa
     8881 aagagcctgt cattttatta tgacaggctc tttaaatgta ttcttttaat taagcttttt
     8941 tctcagtttt tgtttctttc ttttcttctt tttttacttc ttttttttct gcaccatctt
     9001 ttttgcaaca aactttgtct ttactttctt tactgcaagc ttctttgttt tttgctttag
     9061 cttctttttg acaacaatct tttccttttt tgcagtcttt cttatcgcca caagctaatg
     9121 ctacacctac aaataatatt acggatgcag ctaacattaa aaattttttc atgatttgta
     9181 tttttaggtt ttattaaaga gttaatggtg taaaaataag agaatattaa atacaaaaac
     9241 gttaatagta ataaaaaaaa tagtattcta caaaaaaata aaatttctat ctgaaaataa
     9301 gtataaaata tatgtaaata aatttttata aaaattacca ttctgccaaa acagtaacac
     9361 cgccctgtac tttttggttt aattgtacat taatttttgt accaataggt aataaaatat
     9421 ccaccctact tccaaatttt ataaaaccat attctttaca ctggtttact tgttggccta
     9481 cttgtgtata attacatatt ctttttgcaa gtgctcctgc tatttgtttg taaagtatgg
     9541 tgccaaaatt atttttaacc accacactcc aacgttcatt ttctgtagag ctttttgggt
     9601 gccaggcaac aagatatttg cctttatggt atttattata caaaacctct ccactcatgg
     9661 ggtttctgtt tacatgcaca ttggcagggc tcataaaaat acttacttgt attcttttgt
     9721 cattaaaata ttcaacatct gttgtttctt caattacaac aacttttcca tctgcagggc
     9781 aaattatttt attttcatca atagttagtt ttctattggg tattctaaaa aacgaaataa
     9841 taaacaataa aaaaaataga gtaataaaaa aaactaacaa tgcaagccat ggcattgatg
     9901 cactaaaaaa gtaaaaagaa acaacattaa aaactccaaa aaataaagca gtaattccaa
     9961 tggtttgata accttctcta tgtattgtca tttacaaatt ttgaactgca aaaataatgt
    10021 tatttgttta tttaatcagc taatctatcc tgtttagtat tattccacat tttgcaatta
    10081 aataataatt attttatagg ttaaagcaac tgcctattca attttacttt acttttgtac
    10141 gacctctgaa agtttttctt tttattattt acatttttca caaggtcata tcaggcttta
    10201 ttcaagtttc attaattata ttttactgtg tatatggtaa agtaaattat tgttttataa
    10261 tgccttttaa aaaatatttt ttaattgttg aaataaaaaa ataatggcaa agtacatttt
    10321 tgttacaggt ggtgttacaa gtagtttagg taaaggcata attgcagcaa gcctggcaaa
    10381 gcttttacaa gcaagaggtt ttaaagttac cattcaaaaa tttgatcctt atataaatgt
    10441 agatcctggt acattaaacc cttatgaaca tggggaatgt tttgtaacag aagatggtgc
    10501 cgaaacagat ttagatcttg ggcattacga aaggttttta aatattttca catcacagtc
    10561 aaataatgta acaactggca gaatttatca aactgtaatt aataaagaac gtgctggaga
    10621 gtttttagga aaaacagttc aagtggtacc acatattacc gatgaaataa aaagaagaat
    10681 gcaattaatt ggtaaaactg gcgagtatga tattgtaatt actgaattgg gaggaacagt
    10741 tggcgacatt gaaagtttac cttttattga agctgttcgt cagttacaat gggagttgcc
    10801 cgatgaagat gttgtggtgg tacatttaac tttagtacct tatttaaaag cagccaaaga
    10861 attaaaaacc aaacctacac agcatagtgt aaaaatgttg agcgaaactg gagtacaccc
    10921 agatgttatt gtttgtagaa cagaagaacc tttaggaaat gatataaaaa gaaaaattgc
    10981 tttattctgt aatgttaaac aagaggcagt aatagaagct atggatgcta gtactattta
    11041 tgaagttccg ttattaatgc ttagagaaaa gttagattta ttttgcttga aaaaattaaa
    11101 cataaaacaa tttaacgacc cgcaattaga taaatggaaa gcatttttag ataaactaaa
    11161 atatcctaaa gcaaaaatta ctattgggct tattggtaaa tatgtagagt tgcaagatgc
    11221 atataaaagc attttagaaa gctttattca tgcaggtgca attaatgaag taaaaattca
    11281 ggttgttaat attcacagcg agtttataac taaagataat gtggcagaaa aattagaagg
    11341 gttagatggt ttgttggtgg cacctggttt tggatcaaga ggtattgatg gtaaaatatt
    11401 tgcagtgcaa tttgccagag aaaatagctt gccttttttt ggcatttgtt taggtatgca
    11461 aatggcagta attgagtatg gaagaaatat tttaggttta aacgatgctc atagtgtaga
    11521 aatggaacca actacagtaa gccctgttat taatatgatg gaagagcaaa agaaaattaa
    11581 aatgatgggt ggcaccatgc gtttgggagc ttacccgtgt aagctacaag aaaaatcttt
    11641 ggcttataaa atttatggaa atagtcaaat tagcgaaagg catagacata gatatgaatt
    11701 taataatgat tatttagaga tgtatgaaca agcaggaatg atagcaagtg gtaaaaatga
    11761 agaaacaggt ttagtggaaa taatagaaat tccaagccac ccatttttta ttggtgttca
    11821 gtttcatcca gagcttaaaa gcactgtaga agagccagct ccattatttg ttcattttgt
    11881 aggagcagca aaaaaatata acgaaaccaa aagtgcctta atcagtagtt tacttcaaag
    11941 agaaacaatt taaccgtgtc ttgtcctgaa atagggttac ccataaaaaa catcaactag
    12001 atactttcca aagacactaa agttatacta attaaactaa tgtgtttatc taaaatatat
    12061 ggaagataga tttctattat tactaattct gataatggtg atgattggca ttgtgatgct
    12121 tgccaaaaaa attcgtgtag catatcctat tttgttggtc gtggcaggat tgcttattag
    12181 ttttattcca ggtatgccaa caattgagct gagaccggaa cttattttta tacttgttct
    12241 tccgcctatt ttatacgaag cagcatgggc ttgttcattt cgagaattgt ttaaatggag
    12301 aagaattatt ctcagttttg ctttctttgg tgtatttatc acagcaataa gcgttgcatt
    12361 tacggcttat tggtttatcc ccggatttac cttggcgttg ggatttttat taggcggaat
    12421 tgtttctcca cccgatgccg taagtaccaa tgctattaca aagtttgtaa aattgcccaa
    12481 acgaatatct acgattttag aaggtgagag tttattgaat gatgcttctt cattaattat
    12541 tattcagttt gcaattctgg cagttgttac cggaaatttt aatgcgtcta aagcatcttt
    12601 acaatttgca tggatggtga taggaggcgt agcttgtgga ttgttatttg ggtggatatt
    12661 tgttaaagca cataaaaaat tacctaccga tgccaacatg gatgtgttat taacactcat
    12721 agctccctat ttaatgtaca tatctgctga agaaatacac acttcgggag ttttatcggt
    12781 tgttgctggc ggtttatttg tatctcaaaa aaggtatgat tttcttagct ccacatcaag
    12841 ggtacgaggt actaatgtat ggcagagctt tgttttcttg ctcaatggaa ttgtttttat
    12901 acttattgga cttgacctgc cacaaattac agccggtttg gaaggtacga atataacaac
    12961 tgctattggt tatggaattt tgattaccat agtcgtaatt ttagttagac tattaagttt
    13021 ttacggagct gttggcgtaa caatgattat gagaaatttt attacagtag cagaccgaca
    13081 aaatcccggg tttaaaggtc cttttatttt aggatgggct ggaatgcgtg gtgttgtatc
    13141 cttagcagca gctttatcaa ttccttttta tttggacaat ggcgaagttt tccctcaaag
    13201 aaatttaata ctttttatta cctttattgt catcctactt accttggtta tacaaggact
    13261 tactttacca ttcttaataa aaagagctaa actaaaggag atagactatc cgcagcctga
    13321 agatgaggtg tcggcttatt tggataaaga attgctgaag atttcgattg attatttacg
    13381 tagtgttttt ccggagaagg aacaaaaaag caaacgcttt catgaaatat tagtgaacgt
    13441 acaagagcaa ttaagcgaaa acaacgaaat taaattagac tcaaaaagca cccatattta
    13501 ttgcgagctt ttaactttac aacgaaaaca acttttacaa atcaataa
//
LOCUS       DAOQHP010000014        13449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2225_length_13449_cov_3.561072, whole genome shotgun sequence.
ACCESSION   DAOQHP010000014 DAOQHP010000000
VERSION     DAOQHP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_13449_cov_3.561072"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLY81_00995"
     CDS             complement(<1..926)
                     /locus_tag="PLY81_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28835.1"
                     /translation="MKKSLLLGLLFLISTIGWSQTISVAQADKAGSSSNVISGAGAYH
                     ALEAIYLDAEIGASNFIGVGNEIQKIRFAANTNSTGTFPGSIAGYSIYMKNVAAGTKT
                     FTAGSYSLSGYTLVYTGALSATSAGAPYSFTFDAEVTLTTPFARTAGNNLQVLILRNS
                     GIAATGYAWDCSNGNSVDGGTANTSRRYNGATQAVENSTSLSVSAFRPAIQLIRPVSN
                     DATVTVDGATLPTASCFSTPQTVIAYLKNSGTSTIAIGGASVKIDVTGANTYTTTVNN
                     TGALAANASEMIIFSGINLNNAGVNNFKIYST"
     gene            complement(1041..2072)
                     /locus_tag="PLY81_01000"
     CDS             complement(1041..2072)
                     /locus_tag="PLY81_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNF28836.1"
                     /translation="MAYFNLTDTLNFLSKLTFRRAWNAYKVWLSFQISKYSGKPIQWG
                     YPISISFEPTTSCNLRCPECPSGLREFTRPTGMLQNSFFKKTIDEIHKELLYLIFYFQ
                     GEPYLNPNFLEMVKYAHDKGIYTATSTNAHYLTEEKAKKTVESGLDRLIISIDGTTQD
                     VYQQYRRGGKLEKVLQGAQNIVKWKKELNSKTPFIFFQFLVVKPNEHQIEEVKKLGKE
                     IGVDQVRFKTAQVYDYENDPNNLIPTIEKYSRYKKDKTGSLKVKSGMHNYCWRLWHAN
                     VITWDGLVVPCCFDKDAMHRLGNLQTQNFKDIWHNNNYKHFRAELMKSRKNIDICANC
                     SEGLKVWED"
     gene            2196..3320
                     /locus_tag="PLY81_01005"
     CDS             2196..3320
                     /locus_tag="PLY81_01005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF28837.1"
                     /translation="MRIVINTKMLPFNNTCIYRNYMQVMINKLVSRYKHYQFYIITED
                     ENNFEITEAENCIVKIISLKSTNSIALSYWYNIKLATFIKKVKPSIVWHLDSRCIVGL
                     KTPQIITIAHLSFLYQTNNLSNTARIMYSLYQKKWIQKASATITFSLFTQKAIFENYR
                     INNKVEFISPVISSTYNPITYVEKEKIQQTLTGDEEYFLYFGSFNTQHYLVQLLKAFT
                     LFKKRLQSSMKLVLIADAFSNTTILKEKLKTYKFKEDVLLIENASEELLQKITSAAYA
                     FIYTYEHTSIAFNALQAMQAGVPIACSNTGCLAELFNNVALFFDTNNEKNIAEQMMML
                     YKDEGLRNNLIENGIKKAQEFNWDKSINETITIIEHYAMP"
     gene            3359..3706
                     /gene="gldC"
                     /locus_tag="PLY81_01010"
     CDS             3359..3706
                     /gene="gldC"
                     /locus_tag="PLY81_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNF28838.1"
                     /translation="MEKSTITVDVILDENKIPEQISWKASNSSAENKQFAKAVLLALW
                     DGAEKTALRIDLWTKDMMVDEMSDFYYQTFMGMADTYGRATNNQEMVNDLKNFAKSFY
                     DKFRDQQLKENKL"
     gene            3713..4165
                     /locus_tag="PLY81_01015"
     CDS             3713..4165
                     /locus_tag="PLY81_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNF28839.1"
                     /translation="MSLEIQIMGEMKEAMKAKNEAVLRGLRAIKAEIIKAKTEPGANG
                     QINEDTELKLLQKLVKQRKDSFEIFTQQNRADLAQKEKEEIEVIEKFLPKQLTEAELE
                     QVITTIITNSNATSAADMGKVMGIATKQLAGKADGKVISTIVKKLLSV"
     gene            4195..4731
                     /locus_tag="PLY81_01020"
     CDS             4195..4731
                     /locus_tag="PLY81_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNF28840.1"
                     /translation="MLIDIIVLVLLILAIFKGYTKGFIVAIFSTISIFIGLLVALKLS
                     AVTANYLQQSNIKIPIKWLPFLSFMLVVVATIILVRVGSAIIEKTVEFALMGWLNKIA
                     GIALYILLHFSVLSILLFYLIEMNLLSANTVQQSKTYFFIQPIAPKAIQVIGSITPMF
                     KNIFEQLEIFFSSIASNA"
     gene            4911..5669
                     /locus_tag="PLY81_01025"
     CDS             4911..5669
                     /locus_tag="PLY81_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF28841.1"
                     /translation="MNYTIHQQDKFKYLEEGDGEPLMLLHGLFGALSNFADLVEHFKK
                     THKVIVPMLPLFELDIFHTTVGGLQKFVHKFIEARGYNNIHLLGNSLGGHVALVHVLK
                     HPEKIKTLTLTGSSGLFENGMGDSYPKRGDREYMRKKTELTFYDPAMATEELVNEVFE
                     ITNNRLKVIKIIALAKSAIRNNLGEELNQIKQPTLLVWGNNDTITPPFVGQEFNKLIP
                     NSELHFIDKCGHAPMMEVPLEFNKILDTFLSKNK"
     gene            5712..6371
                     /locus_tag="PLY81_01030"
     CDS             5712..6371
                     /locus_tag="PLY81_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF28842.1"
                     /translation="MLAKQIIQTNFPIIQLNDKVIVALRILDDFDIQHLPIISEDKFV
                     GIISKDDLLDADENNIIATLQNYFNTAFVKTEEYFLSAIKLAAQYQLSAIAVVNEQIE
                     LQGIIPLNELVNATALYLSCNEPGGMIILETEKRHFSFGEISRLIETNDSYITQLNTY
                     VEQDTGLFIVTIKVNTIEISDIVATLQRYDYTIRYYFGEEQYNNELKENLNQLMFYIN
                     M"
     gene            6390..6857
                     /locus_tag="PLY81_01035"
     CDS             6390..6857
                     /locus_tag="PLY81_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNF28843.1"
                     /translation="MHSNKIEFIVQSFPALLKTANENSKPLWGKMNLQQMVEHVTEFF
                     TISTNPLQQNIITPVDLLPKYKAFILSDKEFKENTQAPVELIPVEPKPLINASIEIAI
                     NLLTQEITKFVQYFHLHKNAQTIHPVFGSLNFDEWVLLHYKHVLHHCKQFGLV"
     gene            complement(6860..7831)
                     /locus_tag="PLY81_01040"
     CDS             complement(6860..7831)
                     /locus_tag="PLY81_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF28844.1"
                     /translation="MILVTGATGLLGNELIQQLITQGKKIRALFYHTNPTLLHENIEW
                     KQVDILDIVDLEEAMTGITQVYHCAALVSFNKAQKNYLHQINIEGTKNIVNACLQEKV
                     EKLLYVSSVAALGRIREDKPIDETMNWTEETSNSEYGKSKYLAEMEVWRGIGEGLNAV
                     IINPTIILGASNWSKGSTEIFKTAYNEFPWFTNGTTGFVDVKDVAKAMQLLMNSNINA
                     ERFILSSETMTYKDIFSTIAKAFNKKPPYKKVTPFLAALVWRWEAIKGKLTGKHPLLT
                     KETAKTAQTKASFNNSKLLNFLPEFKYNKIDETITRICCELKAMYKL"
     gene            complement(7835..8254)
                     /locus_tag="PLY81_01045"
     CDS             complement(7835..8254)
                     /locus_tag="PLY81_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNF28845.1"
                     /translation="MQTEFLSILKKNNLSVTESRMKILELFAQANGALAHNDIEKKSK
                     LQFDRVTIYRTLQTFVEKGIIHTIPTADNSVLYALCKDECTQGHHNDNHVHFICDSCN
                     VTYCLEHSNSPSVILPKGFKQNRVDVVVSGICLKCNA"
     gene            complement(8290..8946)
                     /locus_tag="PLY81_01050"
     CDS             complement(8290..8946)
                     /locus_tag="PLY81_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF28846.1"
                     /translation="MMKKKKWIFYVTFFVGLILVFWLALYLFTDTFNQSRLVNRSVVK
                     PFTFYKEDGSLFTKADMAGKVCVVEFFFTTCPNICPEMNTNMKKVYEAFKDEPNFLIL
                     SHTCDPKRDSVPVLKKYADSMQVNTQVWAFLTGSKDSLYNMARESYGIDDPKSVVNNI
                     EDDFLHSQFFALVDKNGNVRGQVYDGLKQDEIKQLKKDIKELLNDKGNRRGFSNNLFN
                     "
     gene            9032..9424
                     /locus_tag="PLY81_01055"
     CDS             9032..9424
                     /locus_tag="PLY81_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HNF28847.1"
                     /translation="MAFKINWDALGISTSIACAIHCAVLPLVVTSLPLFGINIIHNLW
                     FEFSMIGIACCIGIYSLYHGYKKHHHSFFPIFVFITGIIFLFAKQIWHEFEIWLLIPA
                     VIFIVTAHFINYRSCRIHKHAHKDDCNH"
     gene            9440..10618
                     /locus_tag="PLY81_01060"
     CDS             9440..10618
                     /locus_tag="PLY81_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF28848.1"
                     /translation="MLDILKEMPWVTAIILTAWLPVIIITLLHLINRKDISITRKIVW
                     LIIGLIPIIGLLIYGLINYKVKKSIVWLTVLATIISLITVWYFIFYQPSIAKRDVTKE
                     AAIALSVTSLLKEFEVNETEANIKYTNKVIEVSGVVEKILNDETGMVIFLQTNIEGKS
                     FSGRLKAKQATQENATITLKGILTGYILGQIQLNEAVITNGANTALSNEPTSTKDSTI
                     NLKVPIKKDSIKVVQPTEKIYSSSKGLIKFISITAVEDIEASNNQVISTISSNGKVQF
                     AALIKGFLFENELMQKHFNEDKYMHSDKFSKSEFDGTITNINSINFNTTGKYKVNAVG
                     NLTIKGITKKITTEGTIDVNNNTILLHSIFKLKIKDFGIITDEIAEQIDIVVSATYKK
                     "
     gene            complement(10800..12530)
                     /locus_tag="PLY81_01065"
     CDS             complement(10800..12530)
                     /locus_tag="PLY81_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNF28849.1"
                     /translation="MKKLLLALVIHCSVVNFSIAQDDMKSVWSIKLEHKAELNEFDEA
                     SGILVSSNEKEISIVDATTGKVKWTKDFKTLCDGIMKKVDERIPMWEAKCIFLFDRKS
                     GKDQMAVIDIETGQMLWSSNKYEGVTEASIAYVPEVGMFAIANKKAFSMVKARTGEEV
                     WETQGFKGTLAKYIYNASDNSIIALNYNPAAAGEGILGGMGKLFAGFGAFKSQLTKFS
                     VKNGDILWQTDIKGAVEKEIATRKILAKLKIVDNDKLMLIMQGMQMFDLNNGSLLWGV
                     THSEEVLKESKRGIPSGYSGSKLVKSAVYGAVADPLIDGNDVYLFDMQNKKNQYVSKY
                     DLHTGKLIWKSKELKKLTIAPSLYKVGGKIIIQIGGWAQVQGIVEQKSNYGGGFGGSG
                     FSVSTYKRVKFYKEFSPYGVEALDATNGELVWRSERFSKGVTNSFVHNGELVVASGKE
                     LYNLNPDSGTEKYSESVKDDGIKETEQIFKVGDNVVVVCSKGMSNYVIADGKKNWTVK
                     TKKGDLSTVNNGMAFYITEKNDFVVIDLTTGKYTTYDARKDSDSEIYEDGQYILVFEK
                     DKVTKLATKQ"
     gene            complement(12541..>13449)
                     /locus_tag="PLY81_01070"
     CDS             complement(12541..>13449)
                     /locus_tag="PLY81_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28850.1"
                     /translation="ISPSGKYIVESWDADKQTFKDVPSIVHRRTELKNAYRAKKILVI
                     YETSNLDKPIAYSNDEIDVVTNMQFSPDEKFVYLQMQLAGQENSTVPNPYAFQRVNIA
                     TGEIDKSFGIKANYCKVDFENNKVTSLVAGGNIGFLKQVKVQDMNNAEDLSYFETRYK
                     FMKTSTMFSPIALVSKSSLAYVYYEKNLYEWDYKILKKYFKKGATATDEELAIMADSL
                     LDASLEDPNSKISKEIKKKEISGNYIMDITITGPKGAVATIFCESDEKTNIPMQNALK
                     DIIKKQEFDLNLPKNKRLKFRYTFQL"
BASE COUNT         4611 a         2154 c         1914 g         4770 t
ORIGIN      
        1 attgtactat aaattttaaa attgttaaca ccagcattgt ttaagttaat accagaaaaa
       61 attatcattt ctgaggcatt tgcagctaaa gcaccagtgt tgtttacagt agttgtgtat
      121 gtattagcac cagtaacatc tatttttaca gaagctccac caatagcaat tgtagatgta
      181 cctgaatttt ttaaatatgc aataacagtt tgtggtgtgc taaagcaaga agcagttgga
      241 agagtagcac catctacagt aacagtagca tcgttagaaa caggacgaat taattgtata
      301 gctggtctaa atgcagaaac agataaagat gtactgtttt caaccgcttg tgttgcacca
      361 ttatatcttc tagaagtatt tgcagtaccc ccatctacag agttgccatt agaacaatcc
      421 caagcatacc cggttgcggc aattccacta tttcttaata ttaaaacttg taagttattg
      481 ccagcagtac gtgcaaaagg tgttgttagt gttacttcag catcaaaagt aaatgaatat
      541 ggtgccccag cagaagttgc acttaaagca ccagtataaa ccaaagtata gccagataat
      601 gaatatgaac ctgctgtaaa tgttttagta cctgcagcca catttttcat ataaatacta
      661 tagccagcaa tagagccagg aaaagtacct gtactatttg tattggctgc aaatctaatt
      721 ttttgtattt cattgcccac accaataaaa tttgaagcac caatttcagc atctaaataa
      781 atagcttcta atgcatgata agctcctgca ccagatataa cattactaga acttcctgct
      841 ttgtcagctt gtgcaactga aatagtttga gaccaaccta ttgtgcttat taaaaacaat
      901 aagcctaaga gtaaactttt tttcataatt agttttttaa gtaaaaaaaa tgataaataa
      961 gggtacaaaa ttagctaaac tattttaata aaaaattatt ttattttaaa gtgggttatt
     1021 acattgttaa agtaaattaa ttagtcttcc caaaccttta acccttcact gcaattggca
     1081 catatatcta tattttttct gcttttcatc aactcagctc taaaatgttt ataattgttg
     1141 ttatgccaaa tatctttaaa attttgcgtt tgtaaattgc ccaaccggtg catagcatct
     1201 ttatcaaaac agcagggcac cactaaacca tcccatgtaa ttacatttgc atgccaaagc
     1261 cgccagcagt aattgtgcat accacttttt acttttaaac tgcctgtttt atctttttta
     1321 tacctactgt atttttcaat agttggtatt aaattattgg gatcgttttc ataatcatac
     1381 acttgtgctg ttttaaacct aacctgatca acaccaattt cttttcctaa ttttttaact
     1441 tcttctattt ggtgttcgtt gggtttaacc actaaaaatt gaaaaaagat aaacggagtt
     1501 ttactattca actctttttt ccattttaca atattttgtg caccttgtaa tactttttct
     1561 aattttccgc cccttctgta ttgttgataa acatcttgtg tagtgccatc aatagaaatt
     1621 attaacctat ctaacccgct ttctactgtt tttttagctt tttcttcggt taaataatga
     1681 gcatttgtac ttgtggctgt ataaattcct ttatcgtggg catatttaac catctctaaa
     1741 aaattagggt ttaaataggg ttcgccttga aaataaaaaa ttaaatacaa cagctcttta
     1801 tgaatttcat caatggtttt tttaaaaaaa ctattttgaa gcattcctgt tggtcttgta
     1861 aattctctta aaccacttgg acattcaggg catcttaaat tacaacttgt tgtaggctca
     1921 aatgaaattg aaattgggta gccccattga attggctttc ctgaatattt acttatttga
     1981 aaacttaacc ataccttata ggcattccat gctctgcgaa atgtaagttt tgataaaaaa
     2041 tttagagtgt cggttaaatt aaaataagcc ataagtaagt tgtaaaaata agcatgtagg
     2101 cttcagttta caattaaagc ttaaaagaaa ttcctgcttt agcaaattag taatttaaat
     2161 tacattctac actaacaaac tttacatttg cccttatgcg tattgttatc aatacaaaaa
     2221 tgcttccgtt taataatacc tgtatataca gaaattatat gcaggtaatg attaataaat
     2281 tagtaagcag gtataaacat tatcagtttt atattattac cgaagatgaa aacaattttg
     2341 aaataactga agcagaaaat tgtattgtaa aaataatttc tttaaaatct acaaacagta
     2401 ttgccctttc ctattggtac aatataaagt tggctacttt cataaaaaaa gtaaaaccct
     2461 ctattgtttg gcatttagat agtaggtgta ttgtaggttt aaaaacccct caaattatta
     2521 ctattgctca tttatctttt ctgtaccaaa caaataactt aagtaacaca gcaaggataa
     2581 tgtattcctt atatcaaaaa aaatggatac aaaaagcttc tgctacaatt acttttagtt
     2641 tatttaccca aaaagcaatt tttgaaaatt atagaattaa taataaagta gaatttattt
     2701 ccccggttat ttcttcaaca tataatccta ttacttatgt tgaaaaagaa aagatacaac
     2761 aaacacttac cggcgacgaa gaatattttt tatactttgg tagttttaat acccagcatt
     2821 atttagttca gttacttaaa gcttttactt tatttaaaaa aagattacag agcagtatga
     2881 agttagtttt aattgcagat gcttttagta acacaaccat tttaaaagaa aaattaaaaa
     2941 cctataagtt taaagaggat gttttgttaa tagaaaatgc ctcagaagag ttactacaaa
     3001 aaataacatc ggcagcctac gcttttattt atacctatga gcacactagc attgcattta
     3061 atgctttaca ggcaatgcaa gctggggtac ctattgcttg tagcaataca ggctgtttag
     3121 ctgagctgtt taacaatgta gctttatttt ttgataccaa taacgaaaag aatattgccg
     3181 agcaaatgat gatgctttat aaagatgaag gtttgaggaa taatttaatt gaaaatggaa
     3241 ttaaaaaggc acaagaattt aattgggata aaagtattaa tgaaacaata accattatag
     3301 aacattatgc aatgccataa tagttttaca tttgccatta ttttttaaat acacttttat
     3361 ggaaaaatca actataacgg tagatgttat tttagatgaa aataagatac ctgaacaaat
     3421 aagttggaaa gcaagcaata gtagtgcaga gaataaacag tttgccaaag cagtactact
     3481 agctctatgg gatggagccg aaaaaactgc tttaagaatt gacctttgga ctaaagatat
     3541 gatggtggat gaaatgtcgg atttttacta tcaaacattt atgggtatgg cagatactta
     3601 tggcagagcc accaataacc aagaaatggt aaacgactta aaaaactttg ctaaaagttt
     3661 ttatgataaa tttagagacc aacaattaaa agaaaataaa ttataattaa ttatgagctt
     3721 agagatacaa ataatgggcg aaatgaaaga ggccatgaaa gctaaaaacg aagccgtttt
     3781 acgtggctta agggctatta aagcagaaat tataaaagct aaaacagaac ctggtgcaaa
     3841 tggacaaata aacgaggata cagaattaaa acttttacaa aaattagtaa agcaaagaaa
     3901 agatagtttt gaaattttta cacaacaaaa ccgtgccgac cttgcccaga aagaaaaaga
     3961 agaaattgaa gtaattgaaa aatttttacc aaagcaatta accgaagccg aattagaaca
     4021 agttattaca actattatta ccaattctaa tgcaaccagt gcagcagata tgggcaaagt
     4081 aatgggtatt gcaaccaagc aacttgcagg caaagcagat ggtaaggtta taagtactat
     4141 agtaaaaaaa ttgctatcgg tttaatgtta ttttaagcca cttatttttt cagtatgcta
     4201 atagatatta ttgtattggt actattaata ttggcaattt ttaaaggcta tacaaaggga
     4261 tttatagtag ccatattttc aactataagt atttttatag ggttgcttgt tgccttaaag
     4321 cttagtgcag ttactgcaaa ttatttacaa caatcaaata taaaaatacc aattaaatgg
     4381 ctgccatttt tatcatttat gttggtagta gtggcaacaa taatattggt tagagttggt
     4441 tctgctatta ttgaaaaaac ggttgagttt gcattaatgg gctggttaaa taaaattgca
     4501 ggaatagctt tatacatact tttgcacttt tcagttttaa gcattcttct attttatttg
     4561 atagaaatga acctcctttc tgccaataca gtgcagcaat ctaaaactta tttttttata
     4621 cagcctattg caccaaaggc aattcaggta ataggctcta ttacaccaat gtttaaaaat
     4681 atttttgaac aacttgaaat atttttttct tctatagcaa gcaatgctta attagtaaaa
     4741 aataattatc cttttatata attatgctta tttttagttg agcaaaaact ataacttagc
     4801 aagtagggtg attttaatat ttaaatttaa taattaagta aagaaactat tctacatttt
     4861 tcctttaatt tgtaaaattc tcatagcccc aaatagcaac caattacaat atgaattata
     4921 ctatacacca acaagataaa tttaagtacc tggaagaagg agatggagag ccattaatgt
     4981 tacttcatgg cttatttggt gcactaagta actttgcaga tttggtggag cattttaaaa
     5041 aaacgcataa ggttattgtg ccaatgttac cactatttga gttggatatc tttcatacta
     5101 cagttggtgg tttacagaaa tttgttcata aatttattga ggccagagga tataataata
     5161 ttcatttatt gggtaactct ttaggcggcc atgttgcttt ggtgcatgta ttaaaacatc
     5221 cagaaaaaat aaaaacttta acccttactg gtagtagtgg tttatttgaa aacggcatgg
     5281 gcgatagtta tcctaaacgt ggagacaggg agtacatgag aaaaaaaaca gaacttactt
     5341 tttatgatcc tgcaatggct acagaagaat tggtaaatga ggtttttgaa attaccaata
     5401 accgattaaa agtgattaaa attattgctt tagcaaaaag tgccattaga aataatttag
     5461 gagaagaatt aaaccaaata aaacaaccaa cgttattggt atggggtaat aacgatacta
     5521 ttacaccccc atttgtaggg caagagttta ataagctaat acccaatagc gaattacatt
     5581 ttattgataa atgtggtcat gcccctatga tggaagtgcc cttggaattt aataaaattt
     5641 tagatacatt tttaagtaaa aacaaataaa tgcagcatct tttgttgtat aattgtttta
     5701 gaatataaaa aatgttagca aagcaaatca tacaaaccaa ttttcctata atacaactaa
     5761 acgataaagt aattgttgct ttaagaattt tagacgattt tgatattcaa catttaccaa
     5821 taatatcaga agataaattt gttggtataa ttagtaaaga tgatttatta gatgctgatg
     5881 aaaacaatat tatagcaact cttcaaaatt attttaatac tgcttttgta aaaacagagg
     5941 aatatttttt aagtgcaatt aaattagcag cacagtacca gctttcagca attgctgttg
     6001 ttaatgaaca aatagagtta caaggtatta ttccattaaa tgaattagta aatgctacag
     6061 ctctttattt aagttgtaac gaacctggtg gaatgataat tttagaaact gaaaaacgcc
     6121 atttctcttt tggtgaaatt agcagattaa tagaaaccaa tgactcctac ataacacagc
     6181 taaatactta tgtagagcaa gatacaggct tatttattgt taccataaaa gttaatacta
     6241 tagaaattag cgatattgtt gcaacccttc aaagatatga ttacactata cgttattact
     6301 ttggagaaga acagtataat aatgaattaa aagaaaattt aaaccaatta atgttttaca
     6361 taaatatgta actcatttta aaaaaatgta tgcactcaaa taaaatagaa tttattgtac
     6421 aatcatttcc tgcattatta aaaactgcta atgaaaacag taaaccttta tggggtaaaa
     6481 tgaatttgca acaaatggta gagcatgtta ctgaattttt tacaatatct accaaccctt
     6541 tacagcaaaa tataattaca cctgttgatt tacttcctaa atacaaagca tttattttga
     6601 gtgataaaga gtttaaagaa aacacccaag ctcctgtaga gttaatacct gtagagccta
     6661 agcctttaat aaatgcatct attgaaattg caattaattt actaacacag gaaataacaa
     6721 aatttgttca atactttcat ttacataaaa atgcccaaac tattcatcct gtatttggta
     6781 gtttaaattt tgacgaatgg gttttattac attacaaaca tgtattacac cattgcaaac
     6841 aatttggctt agtataaatt tatagtttat acattgcttt taattcacaa catattcttg
     6901 ttatagtttc gtctatttta ttgtacttaa actctggcaa aaaatttaat aacttactat
     6961 tattaaaact agcttttgtt tgagctgttt tagcagtttc ttttgttaac aatggatgtt
     7021 tgcctgttag cttgcctttt atagcctccc atctccaaac taaggcagct aaaaaaggag
     7081 ttactttttt atatggcggt tttttattaa atgctttagc tatagtacta aaaatatctt
     7141 tataggtcat agtttccgaa cttaaaataa atctttctgc attaatatta ctattcatta
     7201 aaagttgcat tgctttagca acgtctttta catctacaaa acctgttgta ccatttgtaa
     7261 accaaggaaa ttcgttataa gctgttttaa aaatttccgt tgagcctttg ctccaattgc
     7321 ttgcccctaa aataattgtt gggtttataa ttacagcatt taaaccttcg ccaataccac
     7381 gccatacttc catttctgca aggtatttgc ttttaccata ctcactattg cttgtttctt
     7441 ctgtccaatt catcgtttca tcaatcggct tatcttctct tattctgcct aaagctgcta
     7501 cgctgcttac atacaataat ttttctactt tttcttgtaa gcaagcattt acaatatttt
     7561 tagttccctc aatatttatt tggtgtaaat aatttttttg tgccttgtta aaagaaacaa
     7621 gtgcagcaca atggtacact tgtgtaatac ctgtcattgc ttcttccagg tcaacaatat
     7681 ccaatatatc tacttgtttc cattctatat tttcatgtag caacgttggg ttggtatgat
     7741 aaaataaagc tcttattttt ttaccttggg taataagttg ttgtattaac tcgttgccaa
     7801 gaagccctgt tgcacctgta actaaaatca taatttaagc attacatttt aaacatattc
     7861 cactaactac cacatccact ctattttgtt taaacccttt gggtaaaatt acagaaggtg
     7921 agtttgaatg ttctaaacaa taagttacat tgcaactatc gcaaataaaa tgtacatgat
     7981 tgtcgttatg atgcccttgt gtacactcat ctttacacaa agcatataat acactgttat
     8041 ctgctgtagg aatagtatga atgatgccct tttctacaaa agtttgcaag gttctgtata
     8101 tggttaccct atcaaattga agtttacttt ttttttcaat atcgttatgt gccaatgccc
     8161 cattagcttg tgcaaataat tcaagaattt tcattctact ttcagttaca cttaggttgt
     8221 ttttttttag tatcgataaa aattcagttt gcatgctaca tatttattct atatggtaaa
     8281 aagtttagtt taattaaata aattattaga aaaacccctt cggtttcctt tatcattcag
     8341 caattcctta atatcttttt ttagttgttt tatctcgtct tgtttcaatc catcataaac
     8401 ctggcctctc acatttccat ttttatctac taatgcaaaa aattgagagt gtaaaaaatc
     8461 gtcctcaata ttatttacca cacttttagg gtcatcaata ccatagcttt ctctagccat
     8521 attatataag ctatctttac tgccagttaa aaatgcccaa acttgtgtat taacttgcat
     8581 agaatcggca tattttttta atacaggcac cgaatctctt ttagggtcgc aagtgtgaga
     8641 aagaattaaa aaatttggtt catctttaaa tgcttcatac acttttttca tattggtatt
     8701 catttcaggg caaatatttg gacaggttgt aaaaaagaat tccaccacac aaaccttacc
     8761 tgccatatct gccttggtaa acagactacc atcttcttta taaaaagtaa atggcttaac
     8821 aacagaccta tttactaatc tactttgatt aaaagtatct gtaaacaaat acaatgcaag
     8881 ccaaaaaacc aatattaagc ctacaaaaaa agtaacataa aatatccatt ttttcttttt
     8941 catcatgtgg caaaggtaag cctacatgat aagctaaact gctaaaaaag aatatttttg
     9001 caacattatt gcattttata tctttgcaga aatggcattt aaaattaatt gggacgcttt
     9061 aggaatttct acctctattg cctgtgcaat tcattgtgca gttttaccat tggttgtaac
     9121 cagtcttcct ttatttggta taaatattat acataattta tggtttgagt ttagtatgat
     9181 tggtattgca tgttgtattg gaatttactc attatatcat ggatataaaa aacatcatca
     9241 cagttttttt ccaatatttg tttttattac aggtattatt tttttatttg ccaaacaaat
     9301 ttggcacgaa tttgaaattt ggttattaat acctgctgtt atttttattg taacagcaca
     9361 ttttataaat tatcgctctt gcagaattca taaacatgct cataaagacg attgtaatca
     9421 ttaataaaac ttagttttca tgttggatat tttaaaagaa atgccctggg taacagctat
     9481 tattttaaca gcttggcttc ctgtaataat tattacactt cttcatttaa taaacagaaa
     9541 agatatttca ataacaagaa aaattgtttg gcttattatt ggtttaatac caattatagg
     9601 tttattgata tatggactta ttaattataa agttaaaaaa agtattgttt ggttaacagt
     9661 tttagccact attatttcat taattacagt atggtatttt atattttatc agccatctat
     9721 agcaaaaaga gatgttacta aagaagctgc tattgcacta tcagtaacta gtttactaaa
     9781 agagtttgaa gtaaatgaaa ctgaagccaa tataaaatat accaataaag taattgaagt
     9841 aagtggcgtt gtggagaaaa tattaaacga cgaaactggg atggttattt ttttacaaac
     9901 caatatagag ggtaaatctt tttcaggaag gttaaaagca aagcaagcaa ctcaagaaaa
     9961 tgcaaccata actttaaaag gaattttaac aggctatata cttgggcaaa tacaattaaa
    10021 cgaagctgtg ataacaaatg gtgcaaatac agcattaagc aatgagccaa cttccacaaa
    10081 agattctact attaatttaa aagtgcctat aaaaaaagac agtataaaag ttgtacaacc
    10141 aactgaaaaa atatacagca gctctaaagg gttgataaaa tttatatcta taactgctgt
    10201 ggaggatatt gaagcatcca ataaccaagt aataagtaca ataagcagca acggaaaagt
    10261 gcaatttgca gctttaataa aggggttttt atttgaaaat gaattaatgc aaaaacattt
    10321 taatgaagac aaatacatgc atagcgataa gttttctaaa agtgaatttg atggaacaat
    10381 taccaacatt aatagtataa attttaatac aactggtaag tataaagtga atgctgtagg
    10441 taatctaacc attaaaggta taaccaaaaa aattacaaca gagggaacca ttgatgtaaa
    10501 caataatacc atactgttac atagtatttt taaattaaaa attaaagatt ttggaattat
    10561 taccgatgaa atagctgaac aaatagatat tgttgtttct gccacataca aaaaataaac
    10621 tttttagtat tacaaataaa gaggcagcca ataattgtgg ctgccctgca taggagtata
    10681 attttaaaat gtatcctttt ataaaaccta tctcttttgc tttttattct atactttaat
    10741 ttagcattta caatgtttat tttatagtaa agcttttata ccaattaata aactaatacc
    10801 tattgttttg tagcaagttt agttacttta tctttttcaa aaacaagtat atattgtcca
    10861 tcttcatata tttcagagtc tgaatcttta cgagcatcat aagtagtata ttttccagta
    10921 gttaaatcaa ttacaacaaa atcatttttt tcggtaatat aaaaagccat gccattatta
    10981 actgtactta ggtctccttt cttagttttt actgtccaat tttttttgcc atcagcaata
    11041 acatagttgc tcattccttt tgaacaaact accaccacat tgtccccaac tttaaatatt
    11101 tgttctgttt ctttaatccc atcatctttt acactttcag agtatttctc tgttccagaa
    11161 tcggggttta agttatataa ctctttgccg ctggcaacta ctaactctcc attatgtaca
    11221 aaagagttgg taactccttt agaaaaacgt tcactacgcc aaactaactc tccattggta
    11281 gcatctaaag cctctacacc ataagggcta aactctttgt aaaattttac tcgtttataa
    11341 gtagatactg aaaaaccaga acctccaaat cctcctccat aatttgattt ctgttctaca
    11401 ataccttgta cttgtgccca gccaccaatt tgaattataa ttttaccgcc tactttataa
    11461 aggcttggtg caatggttaa tttttttaat tctttacttt tccaaattaa tttaccagta
    11521 tgtaaatcgt atttgcttac atactgattt tttttgtttt gcatatcaaa taaatacaca
    11581 tcgttgccat caattaaagg atctgcaacg gcaccataca ctgcactttt cactagttta
    11641 cttcctgaat agccacttgg tattcctctt ttagattctt ttagtacttc ttccgaatgt
    11701 gttacacccc aaagaaggct gccattattt aggtcgaaca tttgcatacc ctgcataatg
    11761 agcattaatt tgtcattatc tactattttt agttttgcta atattttacg agtggcaatt
    11821 tctttttcta ctgctccttt aatatctgtt tgccaaagta tatctccatt ttttacacta
    11881 aattttgtaa gctgagactt gaaggcacca aagcctgcaa aaagttttcc catacctcct
    11941 aaaattcctt ctcctgcagc ggcagggttg tagtttaatg ctataataga attatcgctt
    12001 gcattataaa tatattttgc caacgtacct ttaaaccctt gtgtttccca aacctcttct
    12061 ccagttcttg cttttaccat actaaaagct tttttgtttg caatagcaaa cattccaact
    12121 tctggaacat aagcaataga agcctctgtt acaccttcat atttattgct actccaaagc
    12181 atttggcctg tttcaatatc tataactgcc atctggtctt taccgctttt tctgtcaaat
    12241 aaaaaaatac attttgcctc ccacatcgga attctttcgt ccactttttt cataatgcca
    12301 tcacaaagtg ttttaaaatc tttcgtccat tttactttac ctgtagtagc atctactatg
    12361 ctaatttctt tttcattgct gcttactaaa atgccagatg cctcatcaaa ttcatttaat
    12421 tctgctttat gttcaagctt tatactccaa actgatttca tgtcatcttg tgcaatggaa
    12481 aagtttacaa cggagcaatg aattactaat gccaataata attttttcat gtgttttatt
    12541 ttataattga aaagtatatc taaattttaa tcgtttattt ttaggtaagt ttaaatcaaa
    12601 ctcttgtttt ttaataatat cttttaaggc attttgcata gggatatttg ttttttcatc
    12661 gctttcgcaa aatattgtag ctacagcacc tttagggcct gtaatggtaa tatccattat
    12721 atagttgcca gatatttctt tcttttttat ttcttttgaa attttagaat tgggatcttc
    12781 taaactagca tctaatagag aatctgccat aattgctaac tcttcatcgg ttgcggttgc
    12841 tcctttttta aagtattttt ttaaaatttt ataatcccat tcgtataaat tcttttcgta
    12901 ataaacataa gcaagagaag attttgaaac caaagcaatt ggcgaaaaca tagttgatgt
    12961 tttcatgaat ttatatcttg tttcaaaata agataaatct tccgcattat tcatatcttg
    13021 aaccttaact tgttttagaa aaccaatatt gccgcctgca acaaggctag ttactttatt
    13081 attttcaaaa tctactttac agtaattggc ttttatacca aacgatttat caatttctcc
    13141 tgttgcaatg tttactcgtt gaaaagcata cgggttgggt acagtagaat tttcttgccc
    13201 agctaattgc atttgtaaat aaacaaattt ttcatcaggg ctaaattgca tattggttac
    13261 tacatctatt tcatcgttac tatatgctat gggtttatct aagttagaag tttcatagat
    13321 cactaaaatt tttttggctc tgtatgcatt cttcaattct gttcttctat gtacaataga
    13381 tggaacatct ttaaaagttt gtttatctgc atcccaactt tccacaatat actttccact
    13441 tgggcttat
//
LOCUS       DAOQHP010000015        13338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2251_length_13338_cov_4.342468, whole genome shotgun sequence.
ACCESSION   DAOQHP010000015 DAOQHP010000000
VERSION     DAOQHP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_13338_cov_4.342468"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            621..1349
                     /locus_tag="PLY81_01075"
     CDS             621..1349
                     /locus_tag="PLY81_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF28851.1"
                     /translation="MVTLAFYNLKGGVGKTASAVNFAYLAAANGLKTLVWDLDPQGSS
                     SFYFHVKNVKNESKKIFASEMDIKESIQDSTFENLWVIPADISARNADIIFNQSRRKL
                     KSIVQGLKQFDLVILDCPPGISILHDNVFGAADWVLMPNIPTVLSIRSYETIIQYFKE
                     NDIDKAKIKCFFSMVDHRKNLHQETIQAFYNNKLFFKNYIPYLSDIEKMGVHIQPLFE
                     FANSSYAALCYRELWKEISKYCIG"
     gene            1485..4169
                     /gene="metH"
                     /locus_tag="PLY81_01080"
     CDS             1485..4169
                     /gene="metH"
                     /locus_tag="PLY81_01080"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNF28852.1"
                     /translation="MSTAIKPFLRLSGLEPLIIRPETNFVNVGERTNVTGSKKFARLI
                     KENKYEEALSVARQQVENGAQVLDVNMDDALLEGVQAMTTFLNLLQSEPDIAKIPIMI
                     DSSKFEIIEAGLKCVQGKCIVNSISMKEGVEKFIEQAIVCKDYGASVIVMAFDEKGQA
                     DTLERRISISEKAYKILTEQVGFDPQDIIFDLNVFAIATGLEEHNNYGVDFIEATRVI
                     KQKYPLVKISGGISNLSFSFRGNEPVREAMHSVFLYHAIKVGMDMGIVNAGQLVVYDE
                     IDPQLRKLCEDVILNRNNSNNEATDKLIVFAENVKAKDKTEVKDEAWRNESVEKRLAH
                     ALVNGITNYIDADTIEALEKYDKPLDVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSAR
                     VMKKSVAIITPIIEQQKLNNPNEVKQSVAKILLATVKGDVHDIGKNIVGVVLGCNGYE
                     IIDLGVMVPADKILEAAVKENVDIIGVSGLITPSLDEMVHIGKEMKRRGMKQPLLIGG
                     ATTSRIHTAVKIAPEYDNGVVHVLDASRSVTVAGTLLNKDNKQEFINDTKEEYIKIKE
                     NFDNKKTVKQYISYQDALNNKVNIDWENYKPVKPSFTGVKVFEHIDLNELRSYIDWKP
                     YFIAWEMHGNFPAILTDEVVGKEATKLFNDANALLDTIINEKWLTAKAVIGFWEAESN
                     KDEVILPTQNITLQFLRQQIKKAKSQPNISLADFIKPANSNKEVKDYIGAFAVTIHGI
                     EPHIKAFIDNYDDYNKISLQALADRLAESLAEYLHEKVRKEFWGYVKEESLTNEDLIK
                     EKYQGIRPAPGYPACPDHTEKNKLFSLLNATENTGIVLTEHFAMYPASSVCGWYFSNP
                     QSQYYGVGKIQQDQFEDYVQRKKMPKEELERWLSSNLE"
     gene            complement(4193..4945)
                     /locus_tag="PLY81_01085"
     CDS             complement(4193..4945)
                     /locus_tag="PLY81_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNF28853.1"
                     /translation="MELKKFLDKKANQYNQPSFISTDPICIPHLFKQQQDIEIAGFFA
                     AIFAWGNRKIIINKSKELMQLMQMKPYHFILQHNNNDLKKLLHFKHRTFNATDLLYCI
                     AFLKHHYTYHKSLETAFTQWGNTVEEMLIGFHQYFFSLPDYPERTKKHIATPAKNATC
                     KRLNMFLRWMVRRDNNGVDFGLWKNISMAQLICPVDVHVARVAIKLNLIKNKPANWKT
                     AKELTLYLKTLNSKDPVKYDFALFGLGIMEKY"
     gene            complement(4977..5726)
                     /locus_tag="PLY81_01090"
     CDS             complement(4977..5726)
                     /locus_tag="PLY81_01090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF28854.1"
                     /translation="MRKEAWYKDWFSSPYYHQLYQYRDETEAFAFIGTLIQHLKPAKN
                     SIMLDVACGKGRHSIALAEMGFDVTGIDLSLSSIEEASLSEANNLHFFQHDMRLPFWI
                     NYFDYAFNFFTSFGYFKTEREHNNAIRTIATSLKKNGFFVIDYLNTHYAEDTLQPSAI
                     TKVDGTEFHITKWHDEEHFYKQIQITDNTNFTPKHLYTEKIAKFSLGDFNEMLSYQNM
                     QIQEVFGDYKLGSFDIKKSPRMIIIAKKISK"
     gene            complement(5800..6480)
                     /locus_tag="PLY81_01095"
     CDS             complement(5800..6480)
                     /locus_tag="PLY81_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28855.1"
                     /translation="MKTIFFLGFFIISTVAIAQNKIVGTWKNDQAGTLILNANGTGSF
                     EGDAFTYTNNAKKIFVNFDGEVTTYSYYFNNGQLVVSGGDFVQPIAFTNTKSSGNPTG
                     KSKNSGGGGIDKSIVGTWCWTNASNNSYTSSSNTRCIVINADGTYTYTYEGSISGYGG
                     GGYGGSNSQNSDRGTWRVNGNTISVVSESSGAKTYSFQKRNHPKTGDPMIIIDGDAYV
                     TYYKKSPW"
     gene            6720..8108
                     /gene="glmM"
                     /locus_tag="PLY81_01100"
     CDS             6720..8108
                     /gene="glmM"
                     /locus_tag="PLY81_01100"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNF28856.1"
                     /translation="MALIKSISGIRGTIGGKPNETLSPLDIVRFSASYGTWLLNNNTE
                     KIKVVIGRDGRISGPMVQSIVTNTLIALGIDVVDVGISTTPTVEMAVVFHQANGGIII
                     TASHNPKEWNALKLLNNKGEFISAKEGETILDIASKDLFAFASVDKLGTVINDETALD
                     KHIDAILKYPLVDVSAIKKAKFKIVIDAINSSGAFAVPALLKALGVNLKDIVVLNEEV
                     NGKFAHNPEPLPQHLSELCNAVQKQNAHIGIAVDPDVDRLCFVCEDGSLFGEEYTLVA
                     VSDFVLKNKKGNTVSNMSSTRALKDVTEKHGGTHYPSAVGEVNVVNKMKEVNAVIGGE
                     GNGGIIVPDLHYGRDALIGIALFLTHLANEKKSVRQIRNTYPNYFISKNKIDVENGID
                     VKKIFTEIKKKYKNNPINTEDGLKIDFDNDWVHLRTSNTEPIIRIYSESNFETTADNI
                     AKRIMHDIKEIM"
     gene            8134..8883
                     /locus_tag="PLY81_01105"
     CDS             8134..8883
                     /locus_tag="PLY81_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF28857.1"
                     /translation="MKVIPLSEGAFTIDKTKIFVPFNLKVDDLQQRPIGSLLVEVQPF
                     CIVTNQDILLIDTGLGFSKDGEPQLYSNLKQNGIQPQQITKVILSHLHKDHAGGISFR
                     NNIGHYSLSFPNATYYVQKKEFEFAFETGYPSFITDEFAILHNSPNVTWLNGNGNINN
                     YIYYQITGAHSPYHQVFFIKENNETIFFGGDEAPQLQQMKSKFVAKYDFDGRKSMELR
                     QDWWQQGNEENWQFLFYHDIKKPMYQNQPTV"
     gene            9110..10135
                     /locus_tag="PLY81_01110"
     CDS             9110..10135
                     /locus_tag="PLY81_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNF28858.1"
                     /translation="MGLFSFFTQEIAMDLGTANTLIIHNEDIVVNEPSIVALNRNNPK
                     EVLAVGKKALLMHEKTHESIRTVRPLKDGVIADFNAAELMIRELIKLVYPKKPLFPPS
                     WRMVICIPSSITEVEKRAVRDSAEQAGAKEVYMIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRVAGDEFTADIMEALRRYHSLLIGERTAEQIKIHVG
                     AAIKDLDSPPDDMPVNGRDLVTGIPKQIMVSYQEIAEALDKSIFKVEEAILKALETTP
                     PELAADIYRRGLYLTGGGALLRGLDKRLSAKIKLPVHIADSPLKSVVRGTGIALKNYQ
                     RYPFIMK"
     gene            10204..11052
                     /gene="mreC"
                     /locus_tag="PLY81_01115"
     CDS             10204..11052
                     /gene="mreC"
                     /locus_tag="PLY81_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNF28859.1"
                     /translation="MKNIFLFIKRYFVLICFLILQVICIVMLSNSSKTHEAFFASTAN
                     ELTGPINKKYYGIKEYFSLKKINKQLNDENAQLRNLLKTNFEVPDSSVITQKDTLLKD
                     TLNQYRKYTYMPARVVGNTISSNTNYLMLERGSKQGVEKNMSVVSPQGVVGIVVEVSE
                     NYCRVMSLLHKNTRISAMLKKNKSMGDVEWNGEDPHFVTMRKVSKSANVIVGDTVVTS
                     TYSSNFPSNIMIGKVVQVKPDVSTSFNNVTVKTATDFFNIQYVYIIKNIYYHEQEKLL
                     NKRTHE"
     gene            11045..11578
                     /locus_tag="PLY81_01120"
     CDS             11045..11578
                     /locus_tag="PLY81_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNF28860.1"
                     /translation="MSSLLKNIIRFILFVLVQVYILNQVPPLHKYFVPYIYFLYILWL
                     PFNINRLFLLVVAFVYGLTVDFFTGWYGLYTAPTVLIAYLRPFLLNLLIPQETTEQSY
                     IEPSIRSMGATPYSLYIVLLTFIHQFYFVLIEWLQFGSFNLFIGKVAGSTVLSLLLIF
                     IAELLFFRKAKYRTNAA"
     gene            11659..12633
                     /locus_tag="PLY81_01125"
     CDS             11659..12633
                     /locus_tag="PLY81_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNF28861.1"
                     /translation="MSKGPVSKFIQHHYRHFNAAALMDAAKGYETHLTEGGKMMITLA
                     GAMSTAELGISLAEMIRQDKVHIISCTGANLEEDVMNLVAHSHYKRVPNYRDLTPQDE
                     WDLLENHYNRVTDTCIPEEEAFRKLQKHLYKQWKDAEANGERFFPHEFLYKTVLSGDL
                     KQYYEIDAKDSWIIAAAEKNIPIVCPGWEDSTTGNIFASYVIKGELKASTVKSGIEYM
                     VWLTEWYRNNSSGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDINTPKFIVESDATIVAPLIFAWILGW"
     gene            12756..13280
                     /locus_tag="PLY81_01130"
     CDS             12756..13280
                     /locus_tag="PLY81_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28862.1"
                     /translation="MTIIHSCGPYSKLYRSDNTDRNYNGILSDSTYNTLKLFLVKTTS
                     KKLNDTLLIKYDYNNETCWDLLDQKVDDYILGFVTRHKERLQKVEKTRPNVSAFNFRE
                     PGNNLNKIIQLNNLIIIDSSKQLFNLIFSERCTCGSSIIVMPDKRFVFLRSDSHSELL
                     DLSKEQIDEILNKK"
BASE COUNT         4859 a         1858 c         2303 g         4318 t
ORIGIN      
        1 attgggttat aattaagagc agttcaaagt tataaaagcc aaatcatatt gtttgtatat
       61 aaagtattgt cattcagagc ttgcgaggaa tctttaattc tctgtcattc cgagtttacg
      121 aggaatctat ctgttctttg ttgttgctat tcagtagatc cttcgctacg ctcaggatga
      181 caaaagaggg gaataggatg acaagaaagg gtttgtcatt ccgagcttgc gaggaatcta
      241 tctgttctta acctttagat ccttcgcttc gctcaggatg acaagaaagt atatattgtc
      301 attccgagct tgcgaggaat ctctctatat taaaccttta gatgcttcgt tgcctcagca
      361 tgacagcaag tgtatattgt tatcttaaac gaagtaaaat aaaaatgctt gtcattccga
      421 gtttacgagg aatctatctg ttcttaacct ttagatcctt cgcttacgct caggatgaca
      481 aaaaagggaa taggatgaca aaaaatgaaa aaaaaaaaat tgaaactcat tagatccaaa
      541 gaatgatact acaacttaaa tatcaaaaac tttgtgtaga gccaataaat gcttattttt
      601 caatctcaaa aatgtactat atggtaactt tagcttttta taatttaaaa ggaggagttg
      661 gaaaaactgc ttctgctgta aactttgcat atttagcagc agccaatggg ttaaaaactt
      721 tagtttggga tttagaccct caagggtctt cttcttttta ttttcatgtt aagaatgtga
      781 agaatgaaag caagaaaatt tttgccagcg aaatggatat taaagaaagt attcaagatt
      841 ctacttttga aaatttatgg gttataccag ccgatatatc tgcaagaaat gcagatatta
      901 tctttaatca gagcagaaga aaattaaaaa gtattgtaca agggttaaag caatttgatt
      961 tagttatttt agattgccca ccaggtattt ctattttgca cgataatgtt tttggtgcag
     1021 cagattgggt gttaatgcct aatattccta ctgttttatc cattcgttct tacgaaacta
     1081 ttattcaata ttttaaagaa aatgatattg ataaagcaaa aattaaatgc ttttttagta
     1141 tggttgacca tagaaaaaat cttcatcaag aaaccataca agcattttat aataataaat
     1201 tattctttaa aaactatata ccctatttaa gcgatattga aaaaatgggt gtacatattc
     1261 aacccttatt tgagtttgcc aatagtagct atgctgccct ttgttataga gagttatgga
     1321 aagaaattag taaatattgt attggctaat gtaaaattta caggctttat tttttagtaa
     1381 ttatggagga tacaatggtg ttgtttttat tgtttcttat ttagaaaaga aatcagtaaa
     1441 gcatacgtta catttgcgtc ttcaagaaat ttattgagca atttatgagt actgcaatta
     1501 aacctttttt aagattaagt ggattagagc ctttaattat taggccagaa acaaattttg
     1561 taaatgtagg agaaagaacc aatgttacag gctctaaaaa atttgctcgt cttattaaag
     1621 aaaataaata cgaggaagca ttatctgtag cacgccaaca agtagaaaat ggagcacagg
     1681 ttttagatgt aaatatggat gatgctttat tggaaggtgt acaagcaatg actacttttt
     1741 taaacttatt gcaaagcgaa ccagacattg ccaaaatacc cataatgata gactcttcta
     1801 aatttgaaat aattgaagca ggcctaaaat gtgtgcaagg aaaatgtatt gtgaattcta
     1861 tttcaatgaa agaaggagtt gaaaagttta tagaacaagc aattgtttgt aaagattatg
     1921 gtgcatctgt aattgtaatg gcttttgatg aaaaaggaca ggcagatact ttagaacgcc
     1981 gaattagtat atcggaaaaa gcatataaaa ttttaacgga gcaagtaggc tttgaccctc
     2041 aagatataat ttttgactta aatgtttttg ctattgcaac aggcttagag gagcataata
     2101 actacggagt tgattttata gaagctacta gagttataaa acaaaaatat ccattagtta
     2161 aaattagtgg aggcattagt aacctttctt tttcttttag aggtaatgaa cctgtaagag
     2221 aagctatgca ctcggtattt ttataccatg ctattaaagt tggtatggat atgggcattg
     2281 taaatgctgg gcaattagtt gtatatgatg aaatagaccc acaattaaga aaactttgtg
     2341 aagatgtaat tttaaacaga aataatagta ataatgaagc aacagacaag ttaattgtat
     2401 ttgcagaaaa tgtaaaagct aaagataaaa cagaagtaaa agatgaagct tggcgtaacg
     2461 aatctgttga aaaaagatta gcacatgctt tggtaaatgg cataacaaat tatattgatg
     2521 cagatacaat agaagcctta gagaaatatg ataagccctt agatgtaatt gaggggccac
     2581 taatggatgg aatgaatata gttggcgact tatttggtgc aggtaaaatg tttttacctc
     2641 aggttgtaaa atctgcaagg gtaatgaaaa aatctgtagc cattattact cctattattg
     2701 aacagcaaaa attaaacaac cccaatgagg ttaaacaaag tgttgcaaaa attttattag
     2761 caaccgttaa aggcgatgta catgatattg gtaaaaacat tgtaggggtt gtgttaggat
     2821 gcaatgggta tgaaattatt gatttaggcg taatggtacc agctgataaa attttagaag
     2881 ccgctgttaa agaaaacgta gatataatag gtgttagtgg attaattacc ccatcgttag
     2941 atgaaatggt tcacattggt aaagaaatga agcgtagggg catgaaacaa ccattactaa
     3001 ttggtggtgc cactacatca agaatacata ctgcggtaaa aattgcccct gaatatgata
     3061 atggagtagt tcatgtttta gatgcaagcc gtagtgttac tgttgcagga actcttttaa
     3121 ataaagacaa taaacaagaa tttattaatg atactaaaga agagtatatt aaaataaaag
     3181 aaaattttga taataaaaaa acagtaaaac aatatataag ctaccaagat gcattgaata
     3241 ataaagttaa tattgactgg gaaaattata agcctgttaa accaagcttt acaggagtta
     3301 aagtttttga acatatagat ttgaatgaac ttaggagcta catagattgg aaaccatatt
     3361 ttattgcatg ggaaatgcat ggcaattttc cagcaatact taccgatgaa gtggtaggta
     3421 aagaggcaac aaaattattt aatgatgcta atgctttact tgatactatt attaacgaaa
     3481 aatggttaac agcaaaagca gtaattggct tttgggaagc tgaaagtaat aaggacgaag
     3541 tgatattgcc tacacaaaat attaccttac agtttttaag gcaacaaatt aaaaaagcaa
     3601 aaagccaacc caatatttca ttagcagatt ttattaaacc tgccaatagt aataaagaag
     3661 taaaagatta tattggtgct tttgctgtaa ctatacacgg tattgaacct catataaaag
     3721 catttataga taattacgac gattataata aaattagctt acaggcatta gcagataggc
     3781 ttgcagaatc tttagcagaa tatttacatg aaaaagtaag aaaagaattt tggggttatg
     3841 ttaaagaaga aagtttaact aacgaagatt taattaaaga aaaataccaa ggtattcgtc
     3901 cagcacctgg ttaccctgca tgcccagatc atacagaaaa aaataaatta tttagcttat
     3961 taaatgctac agaaaataca ggtattgtac ttactgagca ttttgccatg tatcctgcaa
     4021 gcagtgtatg tggttggtac tttagtaacc cacaaagcca atattatggt gtaggcaaaa
     4081 ttcaacaaga tcagtttgaa gattatgtac agagaaaaaa aatgcctaaa gaagaattag
     4141 aaagatggtt aagctctaat ttagaataaa ccatttttaa cttaaaaata ttttaatatt
     4201 tttccattat acctaaacca aataatgcaa aatcgtattt aacagggtct ttgctgttta
     4261 aggtttttaa ataaagggta agttctttag cagttttcca attggcaggt ttgtttttaa
     4321 ttaagtttag ctttatggca actcttgcaa catgtacatc cactgggcaa ataagctgtg
     4381 ccatagaaat atttttccat aaaccaaaat caacaccatt attatccctt ctaaccatcc
     4441 atcttaaaaa catatttaat cttttgcagg ttgcattttt tgctggtgtt gcaatatgtt
     4501 ttttagttct ttcaggataa tcgggtaaag aaaaaaaata ttgatgaaaa ccaataagca
     4561 tttcctctac tgtattgccc cattgtgtaa aagcagtttc taaactttta tgatatgtat
     4621 aatggtgttt taagaaagct atacagtata gcaaatctgt tgcattaaaa gttctgtgtt
     4681 taaaatgtag aagttttttt aaatcgttat tgttatgttg cagtataaaa tgatatggct
     4741 tcatttgcat taactgcatt aactctttac ttttattaat aataattttt ctattacccc
     4801 atgcaaaaat ggctgcaaaa aaacctgcaa tttcaatatc ttgttgctgc ttaaataaat
     4861 gaggaataca aataggatct gtactgataa atgaaggttg attgtattga ttagcttttt
     4921 tatctagaaa tttttttaac tccatacatt acttttaact acaaaaaagt aagctattat
     4981 ttacttattt ttttagctat aataatcatt cttggagatt tttttatatc aaaactacca
     5041 agtttataat ctccaaaaac ctcttgtatc tgcatgtttt gataagaaag catttcatta
     5101 aaatcgccta aagaaaattt ggcaattttt tcggtatata aatgtttagg agtaaagtta
     5161 gtattatcgg ttatttgaat ttgtttgtaa aaatgttctt catcgtgcca ttttgttata
     5221 tgaaattcgg ttccatcaac tttagtaata gcactaggtt gtaaagtgtc ttcggcataa
     5281 tgcgtattca aatagtctat cacaaaaaag ccattctttt ttaagctggt agcaattgtt
     5341 ctaatagcat tattatgttc acgttcagtt ttaaaatatc caaaactggt aaaaaaatta
     5401 aaggcataat caaaataatt aatccaaaaa ggcaatctca tatcatgctg aaaaaagtgt
     5461 agattatttg cttcgctaag gcttgcttct tcaatagagg aaagagaaag gtcaatacct
     5521 gtaacatcaa agcccatttc tgccaaagca atgctgtgcc tgcctttgcc acaagcaaca
     5581 tctaacatta tactgttttt tgctggcttt aaatgttgta ttaaagtgcc aataaaagca
     5641 aatgcttccg tttcgtctct gtattgatat agttgatgat agtatggcga tgaaaaccaa
     5701 tctttatacc aagcttcttt cctcataaaa cctttttact aaagcaaagg taatgatgta
     5761 agctaaaaag tatttttaaa aaatgcaagt gtaagaaaat taccaagggg attttttata
     5821 ataagttaca taagcatctc catcaataat aatcatgggg tcgccggttt tagggtgatt
     5881 tctcttttga aaagaatagg ttttagctcc cgagctttca gaaacaacac taattgtatt
     5941 gccatttacc ctccatgttc ctctgtcaga attttggctg ttagaaccac cataaccacc
     6001 accaccatac ccacttatag aaccttcgta agtgtaagta tatgttccat cggcattaat
     6061 aacaatgcat ctggtattgc tagaactagt ataactattg ttggatgcat tggtccaaca
     6121 ccaagtaccc acaatagatt tatcaattcc accaccacca ctgtttttag attttcctgt
     6181 tgggtttcca gaactttttg tgtttgtaaa agcaataggt tgtacaaaat cgccaccaga
     6241 aacgactaac tgcccattat taaaataata agagtaagta gttacttctc catcaaaatt
     6301 tacaaatatt ttttttgcat tattggtgta tgtaaaagca tcaccttcaa aacttcctgt
     6361 accatttgca tttaaaatta atgtacctgc ttggtcattt ttccaagtgc caacaatttt
     6421 gttttgagct attgctaccg tagaaattat aaaaaaacct aaaaaaaata tagttttcat
     6481 atcagtttat tttataccta aaattataaa aattaaatac aaaaaggtaa tttattttat
     6541 ctcctccatt ttttgtttaa tggttgctaa acttactgat tttcaacaaa aaattatttt
     6601 tagtagtttt ctgtaactat aaaacttctt taaaaaacac tttactaatg ctctttaatt
     6661 atataaataa aaaaaagtaa gctagttttt ggtatgtttg ccaccttaaa aaaggtttaa
     6721 tggcgttaat aaagagtatt tctggaatta gaggaacgat agggggtaaa ccaaatgaaa
     6781 cactaagtcc tttagatatt gttagatttt ctgcatccta tggcacctgg ttattgaata
     6841 acaatacaga aaaaataaaa gtagttattg gtagagatgg tagaattagt ggcccaatgg
     6901 ttcaaagcat tgtaaccaat acattgattg cattaggtat agatgtggta gatgttggta
     6961 taagcactac gccaactgtg gaaatggcag ttgtttttca ccaagctaat ggaggtataa
     7021 ttattactgc aagccataac cctaaagaat ggaatgcatt aaaactacta aataataaag
     7081 gcgaatttat tagtgcaaaa gaaggcgaaa ctattttaga cattgcaagt aaagatttat
     7141 ttgcttttgc aagtgtagat aaactaggca cagtaataaa tgacgaaact gctttagaca
     7201 aacatattga tgcaatatta aaatatccgt tggtagatgt aagtgctatt aaaaaagcta
     7261 aatttaaaat tgttatagat gcaattaata gcagtggagc atttgctgta cctgctttat
     7321 taaaagcttt gggagttaat ttaaaagata ttgttgtttt aaacgaagaa gtaaacggaa
     7381 aatttgccca taatccagaa cccttacctc aacatttatc agagttatgc aatgctgtgc
     7441 aaaaacaaaa tgcacatatt ggaattgcag ttgaccctga tgtagataga ctttgctttg
     7501 tttgtgaaga tggtagttta tttggagaag aatatacttt agtagcagtt tctgattttg
     7561 ttttaaaaaa caaaaaagga aatactgtga gcaatatgag cagtaccaga gcgttgaaag
     7621 atgttacaga aaaacatggt ggtacacatt atccaagtgc tgtaggcgaa gtaaatgtgg
     7681 tgaataaaat gaaagaagta aatgctgtaa ttggtggaga aggcaatggt ggaattattg
     7741 taccagattt gcattatggt cgtgatgcac ttataggtat tgctttgttt ttaacacatt
     7801 tagccaatga aaagaagagt gttcgccaaa taagaaatac ttatcctaac tactttatca
     7861 gtaaaaataa aatagatgta gaaaacggga ttgatgtaaa aaaaatattt acagaaataa
     7921 agaagaaata taaaaataac ccaattaata ccgaagatgg tttaaaaata gattttgata
     7981 acgattgggt gcatttaaga acaagtaata ccgagccaat tatacgtatt tacagcgaaa
     8041 gcaattttga aactacagca gataatattg caaaacgtat aatgcatgat attaaagaaa
     8101 taatgtaagc atttaaccaa tgcattattt ataatgaaag ttataccatt atctgaagga
     8161 gcatttacca ttgacaaaac aaaaatattt gtaccattta atttaaaggt agatgactta
     8221 caacaacgcc ctattggtag cttattggta gaagtacaac ccttttgtat tgtaactaac
     8281 caagatattt tattaattga tacaggctta ggttttagta aagatggaga gcctcaacta
     8341 tattccaacc ttaaacaaaa tggcattcag ccacagcaaa taactaaagt aatattaagc
     8401 catttgcata aagaccatgc tggtggtatt agttttagaa ataatattgg acattatagt
     8461 ttatcctttc ctaatgctac atattatgtt caaaaaaaag aatttgaatt tgcttttgaa
     8521 acgggttacc catcctttat tacagatgaa tttgcaatac ttcacaacag ccctaatgta
     8581 acttggttaa atggaaatgg gaatataaat aattacattt attaccaaat tacaggagct
     8641 catagtcctt accatcaagt tttttttatt aaagaaaata acgaaaccat attttttgga
     8701 ggagatgagg caccacagct acaacaaatg aaaagtaaat ttgtagcaaa atatgatttt
     8761 gatggtagaa aaagtatgga attaagacaa gattggtggc aacaaggcaa cgaagaaaat
     8821 tggcagtttc tcttttatca cgatataaaa aaaccaatgt atcaaaatca gcccactgtg
     8881 tagcaattat tcaatttaag tttaatacct ttcttcccta aaaaagtaaa tcgcaacatt
     8941 tctttttata aaacattttt aataattatt cataaaagaa ataaaacagt taatttaggc
     9001 aaaaaatgaa gaaacattgt aaaaaacact gctttttcct ttgttaattc aagttatttc
     9061 attcaccttt gttcgctatt attttaaaaa attatataac ctttaataca tgggactttt
     9121 tagctttttt acacaagaaa ttgctatgga tttgggcact gccaatacat taattattca
     9181 taacgaagat attgttgtta atgaaccaag catagtagct ttaaatagaa ataatccaaa
     9241 agaagtttta gctgttggca aaaaggcatt attaatgcat gaaaaaaccc atgaaagtat
     9301 tagaacagtt cgtccattaa aagatggtgt aattgccgat tttaatgcag ccgaattaat
     9361 gataagagaa ttaataaaat tggtatatcc taaaaaacca cttttcccac caagttggag
     9421 aatggtaatt tgcataccat catcaattac agaagttgaa aaacgtgctg taagagatag
     9481 tgcagagcaa gcaggagcaa aagaagtgta tatgatacac gagccaatgg cagcagcctt
     9541 aggtattggt attgatgtgg aagaaccagt aggaaatatg attattgata ttggtggagg
     9601 aacaacaggt attacagtta ttgcattagc aggtattgtt tgcgaccaaa gcattcgtgt
     9661 tgcaggcgat gagtttactg cagatattat ggaagcttta cgccgttatc atagtttatt
     9721 aattggtgag cgtaccgcag aacaaattaa aattcatgta ggagcagcta taaaagattt
     9781 agacagccca ccagatgata tgcctgtaaa tggtagagat ttagttactg gtattcctaa
     9841 acaaattatg gtaagctatc aagaaattgc agaagcatta gataaaagta tttttaaagt
     9901 agaagaagca attttaaaag ctttagaaac cacaccacca gaacttgctg cagatattta
     9961 tagacgaggg ttgtatttaa ctggtggagg tgctttatta cgtggcttag ataaaagatt
    10021 aagtgctaaa ataaaattac ctgtacatat tgcagactct cctttaaaaa gcgttgtaag
    10081 aggtactgga attgcattaa aaaactacca gcgatatcct tttataatga aatagaaaat
    10141 gagatagtac ttttgattta acaagttaaa acaaatgtat tatttcaaaa actgctgcta
    10201 agtgtgaaaa atatttttct ttttattaaa cgttactttg ttctaatctg ctttttaata
    10261 ttgcaggtta tttgtatagt tatgcttagt aacagcagta aaacacacga ggcatttttt
    10321 gcatctactg caaatgaact aacagggcca ataaataaaa aatactacgg cattaaagaa
    10381 tacttctctc tcaaaaaaat taataaacaa ttaaatgatg aaaatgcaca gcttagaaat
    10441 ttgctaaaaa caaattttga agtgccagac tcttcagtta tcacacaaaa agatacactc
    10501 ttaaaagata cactcaacca atatagaaaa tatacttata tgcctgcaag ggttgttggt
    10561 aatactattt cctctaacac caattactta atgttagaaa gaggaagcaa acaaggagtg
    10621 gaaaaaaaca tgagtgttgt atcgccgcaa ggtgttgttg gaattgttgt tgaagtaagc
    10681 gaaaactatt gcagggtaat gagcttatta cataaaaaca ccagaattag tgctatgctt
    10741 aaaaagaata aaagcatggg agatgttgaa tggaatggag aagacccaca ttttgtaact
    10801 atgcgtaaag ttagcaaatc tgccaatgtt attgttggtg atactgtagt taccagcact
    10861 tattcaagca attttccatc caatattatg attggtaaag tggtacaagt taaaccagat
    10921 gtttcaacca gttttaataa tgtaacagtt aaaacagcaa cagatttttt taatattcag
    10981 tatgtgtata ttattaaaaa tatatattat cacgaacagg aaaaactttt aaataaaaga
    11041 acacatgagt agcctgttaa aaaatataat acgctttata ctatttgttt tggttcaagt
    11101 gtacatactt aatcaagtac cacccttaca taaatacttt gtaccctata tttacttttt
    11161 atatatttta tggttgcctt ttaatatcaa cagattattt ttacttgtag tagcttttgt
    11221 atatgggcta actgtagatt tttttactgg ctggtatggt ttatatacag caccaactgt
    11281 tttaattgct tacttacgtc ccttcttatt aaatctactt ataccacaag aaacaactga
    11341 gcagagttat attgaaccaa gcattagaag tatgggtgca acgccatata gtttgtatat
    11401 tgttttacta actttcattc accagtttta ttttgtttta atagagtggt tacagtttgg
    11461 cagctttaat ttatttattg gaaaagtagc tggcagcaca gttttaagtt tattattaat
    11521 ttttattgcc gagttacttt tttttagaaa agcaaagtat agaaccaatg ctgcataaat
    11581 aattaaagca ttcatttttg ttttatttaa taccaagtaa aaaactactt ttgccaacta
    11641 actataaaat atttaatcat gagtaaaggg cctgtttcta aatttataca acaccattat
    11701 cgccatttta atgcagctgc tttaatggac gctgctaaag gatatgaaac ccatttaacc
    11761 gaaggtggaa aaatgatgat aaccctggca ggtgcaatga gcactgcaga attgggtatt
    11821 tcattagccg aaatgattag gcaagataaa gtacatatta ttagttgcac aggagcaaat
    11881 ttagaagaag atgtaatgaa cctcgttgct cactctcatt acaaacgtgt acctaattat
    11941 agagatttaa cgccacaaga tgaatgggat ttattagaaa accattacaa tagagttaca
    12001 gatacttgca taccagaaga agaagcattt agaaaattac aaaaacattt atataagcaa
    12061 tggaaagatg ctgaagcaaa tggcgaaaga tttttccctc atgagttttt atataaaaca
    12121 gttttaagtg gcgatttaaa acagtattat gaaattgatg ctaaagacag ttggataatt
    12181 gctgctgccg aaaaaaatat tcctattgtt tgcccaggtt gggaagacag cacaacggga
    12241 aatatttttg caagctatgt aataaaagga gagttaaaag caagcacagt taaaagtggt
    12301 attgaatata tggtttggct aacagagtgg tatagaaata actcatctgg taaaggagtt
    12361 ggtttttttc aaattggtgg tggtattgct ggcgattttc caatttgtgt tgtacctatg
    12421 atgtaccaag atttagaatg gcacgatgtg cctttttgga gctatttttg tcaaataagc
    12481 gacagcacta catcttatgg ctcttatagt ggtgctgtgc caaacgaaaa aattacttgg
    12541 ggtaaattag atattaatac tcctaaattt attgtagaaa gtgatgctac tatagttgca
    12601 ccattaatat ttgcctggat actaggctgg taaaatagaa agaaaaatta tgtataactg
    12661 tatctgataa aggaaaattg gtagccatct tggaaaattt ttaatttttt gaaatgaaac
    12721 gaaattttca cactttcctt ttagcaattt ttgtattgac aataattcat agttgtggcc
    12781 cttattcaaa actttatcga agcgataaca cagacagaaa ttacaatgga atattaagcg
    12841 actcaactta taatacttta aaattgtttt tagtaaagac aacgagtaaa aaattaaatg
    12901 acactttgtt aattaagtat gactataaca atgaaacttg ttgggatttg ttagaccaaa
    12961 aagtagatga ttacatttta ggttttgtaa caagacataa agaaagactt caaaaagttg
    13021 aaaagacaag accaaatgta tcagcattta actttagaga gccaggaaac aacttgaata
    13081 aaattattca gttgaacaat ttaattatca tagacagttc aaagcaactt tttaatttaa
    13141 tttttagtga acgttgcaca tgtggaagtt caatcattgt aatgccagac aaaaggtttg
    13201 ttttcttgcg ttcagactcg cattctgaac ttttagattt aagcaaagaa caaattgacg
    13261 aaatactaaa taagaagtaa aagaaaagag gctaccagca ggtttggcaa taggctgtct
    13321 gaatgaatat caattagt
//
LOCUS       DAOQHP010000016        12950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2361_length_12950_cov_3.994727, whole genome shotgun sequence.
ACCESSION   DAOQHP010000016 DAOQHP010000000
VERSION     DAOQHP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12950
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_12950_cov_3.994727"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..796)
                     /locus_tag="PLY81_01135"
     CDS             complement(74..796)
                     /locus_tag="PLY81_01135"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF28863.1"
                     /translation="MKNNKINRRNFITNTAKVGIGLTILPTILQAKEDGVVVNFKQQP
                     LQYSYKDLEPYIDAATMEIHYTKHAAGYTKNLNEALKDEYKKSNAITIEDILAKISSY
                     SSKMRNNAGGHYNHELFWQTLTPKSSGKPTQKVLEVINNSFGSFEEFKKLFSQAAATR
                     FGSGWAWLIENDGHLKISSTPNQDNPLMDVAEVKGYPILGLDVWEHAYYLKYQNKRTE
                     YINNWWNIINWEAVEKRLLNKK"
     gene            complement(800..2236)
                     /locus_tag="PLY81_01140"
     CDS             complement(800..2236)
                     /locus_tag="PLY81_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNF28864.1"
                     /translation="MFKKLDTLILRSFVAPFFATFFISLFVLVMQFFWLYIDDLVGKG
                     LEVTTIFKLIAYVTATLVPLALPLAMLLSSIMTFGNLGETFELVAIKSSGISLLRFMR
                     PLLIVAIAISGLAFVFANNVIPVAQLKLAALKYDIIIAKPAFDIKEGVFYNQIKDFAI
                     KIGKKEKDDSTIKNIVIFEKRYGIQDNIIVADSGIMKVSNNKKFLEFVLKDGWRYEEK
                     AQANSATNTDYIRLGFKEYKKIFDLRSLQMNQSADSSFRYDPKMLSIRQLNYSIDSFK
                     TIQQKFIKRNNTEVAETLPFVQYVNATFKQAKPLKHVNNITAIIPDSIRTIIFDNANS
                     KLNIARNTTAIAANDFEFKNESKINHQIEWHRKFTLSFACIVLFLIGAPLGSIIRKGG
                     LGLPLVFSIIFFIIFHMINTFGEKFVEESVASPFVGMWLSSMILLPIGFFLTFKAMRD
                     SQLFNQEFYYRTFKGVREFIKKFKKIKV"
     gene            complement(2392..2775)
                     /locus_tag="PLY81_01145"
     CDS             complement(2392..2775)
                     /locus_tag="PLY81_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNF28865.1"
                     /translation="MSKKQLFTLEFKVRCSPSILFEFLSTPTGLQEWFADKVDEWEGE
                     LSFSWSGGTPDKAVVIDAEQDKFIRYRWLHAPKEEYFEFKIAKTEISNQTILVISDFA
                     EKSEIKDQTQLWDYQVKELLHRLGS"
     gene            3047..4378
                     /gene="ffh"
                     /locus_tag="PLY81_01150"
     CDS             3047..4378
                     /gene="ffh"
                     /locus_tag="PLY81_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNF28866.1"
                     /translation="MFNNLSEKLEIAFKNLKGQSRINDLNIANTIKDIRRALLDADVN
                     FKIAKEFTDKVKVEAIGSKVLHAVSPGQQMVKIVQDELTALMGGEPAEFNISGNPAII
                     LIAGLQGSGKTTFTGKLATYLKDKKGKSPMVVAADIYRPAAIDQLTVLAQQIGVDIYK
                     EPETKDAVLIAQNAIKEAKAKNKNVVIIDTAGRLAIDEIMMTEVANIKAAVNPSEILF
                     VVDSMTGQDAVNTAKAFNDKLDFSGVVLTKLDGDTRGGAALSIKYTVNKPIKFMSSGE
                     KMDTLDVFYPDRMAQRILGMGDITTLVEKAQQQFDEEQAKKLEKKIRKNQFDFQDFLE
                     QLQQIKKMGNVKDLMGMIPGVGKAIKDVEVDNSSFKGIEAIIQSMTLEERANPDLINP
                     TRKSRIAKGCGKDIAEINAFIKQFEQMKKMMSMMNKMPMGGLSNMMKGMKR"
     gene            complement(4502..5197)
                     /locus_tag="PLY81_01155"
     CDS             complement(4502..5197)
                     /locus_tag="PLY81_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF28867.1"
                     /translation="MEGRKYRILLCEDDTNLGMVLKNYLELNDYDVVLERDGRLGLAA
                     FQREKFDICLLDVMMPNMDGFTAAEEIRDIDPDIPLFFLSAKTMKEDIIQGYKLGADD
                     YITKPFDSEVLLHKIKAILKRNEEETKVNENIEFDLGTYHFNPKLRELIREGSTQTLS
                     PKENDLLKMLAEHKNDLLPRERALKKIWGSDTYFNGRSMDVYIAKLRKYLKEDEKIEI
                     VNIHGNGFRLVAP"
     gene            5422..6636
                     /locus_tag="PLY81_01160"
     CDS             5422..6636
                     /locus_tag="PLY81_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF28868.1"
                     /translation="MNIKRKIINDPVYGFITIDHPILFKIIAHPYYQRLRRIHQMALA
                     YLVYPGAVHTRLHHSLGAYHLMCLAITELKAKGTEITEEEEIAVKAAILLHDIGHGAF
                     SHALEKQILNNIHHEELSLKMMKVINSELNGQLDTTIAIFTNNYPKKFLHQLVSGQLD
                     VDRMDYLTRDSFFTGVSEGVIGYDRIIKMLIVHNGELMVEEKGIYSIEKFLVARRQMY
                     WQVYLHKTVVAAEVMLVNILKRVNELSVKDKTGLQTDGALDYFLFEYTNGLNLQVVEK
                     FSELDDSDLSFAIKKWSKHKDFVLSTLCKKLLARNLYKIKLQSKPFNSEEINELQVKA
                     MQQFKVDSSLIPYFVHTGTANNTMYKTEDERINILYKDGTVKDISAIDNPLIHQTISA
                     PIKKFYICLLEP"
     gene            6669..7718
                     /gene="lpxD"
                     /locus_tag="PLY81_01165"
     CDS             6669..7718
                     /gene="lpxD"
                     /locus_tag="PLY81_01165"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF28869.1"
                     /translation="MFTAQQIALLVNAKIEGDPNIIVSSFGKIEEAKEGQLTFFANPK
                     YEDFLYTTTASVVLLNENFVLKLPIKATLLRVADAYSAFAILLNKYQAIQAQQLEGIQ
                     QPVYISPSAKIDEKVFIAAFTYIGENVKIGKNVKLFAGVSLGNNVEIDDNTILHAGVK
                     IYEGCKIGKNVIIHAGTVIGGDGFGFAPQANGSYQKIPQIGNVIIEDFVEIGANTTID
                     RATIGSTIIRTGVKLDNLIQIAHNVEVGNNTVIAAQAGVSGSTKIGKNVMIGGQAGIV
                     GHITVADGSKINGQSGVSKSITEPNQAVTGSPAYDYTAALRSQAVVRKLPELEKRIKE
                     LENQLSQILALESKS"
     gene            7750..8652
                     /locus_tag="PLY81_01170"
     CDS             7750..8652
                     /locus_tag="PLY81_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNF28870.1"
                     /translation="MNTFLKLSDEVANALQQKKPVVALESTIIAHGMPYPKNVETALA
                     VEQTIKNNGATPATIAIINGKCCVGLTAEEIEIFGKEKEVWKVSLRDMPYVISKKLFG
                     ATTVAATMRIAAMAGIKIFVTGGIGGVHRGAEKTMDISADLSEMAETNVAVVCAGVKS
                     ILDIGLTLEYLETKGIPVVTYGQKSFPSFYSTESNYNTPLQLNSSKEIAEMLQTKWAL
                     GLNGSVLIANPINKKDEVPFAVIESHITNALEHAEKQGIKGKETTPFLLQHIAQHTNG
                     NSLEANISLIKNNAVLGAQIAAHL"
     gene            8664..9179
                     /locus_tag="PLY81_01175"
     CDS             8664..9179
                     /locus_tag="PLY81_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28871.1"
                     /translation="MNIFSPYKINSFVMGSNINIELANWRKNINYITLRIFPFVLSVI
                     TVYAVILNFKEMPKAYSYVLLIGIPALSVYMFFTKYSVQTIIKPPYIQVYKNSFRGKE
                     ESNINFNSIHKLTCKIRYGKYGGTFFYLHTSANKKIEFITIPVTNMKKEYTDLIIKEL
                     EAIVGKTVEFV"
     gene            9246..10256
                     /locus_tag="PLY81_01180"
     CDS             9246..10256
                     /locus_tag="PLY81_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNF28872.1"
                     /translation="MKFGVIGSGSWATALAKILTDNGNHINWWIRNTDTISYLHTRKH
                     NPRYVSSAYFNTQQLYMSNEITEVVEKSNVLVIATPSAFTEDVLKQLPSNAFEGKIII
                     SAIKGILPSENDLLNEYLKKHFNVPIENYCTILGPCHAEEVASEKLSYLTISNINESL
                     ALSIAKHFNTEYLNTRVNHDISGVQYAAILKNIYALGAGIAHGLEYGDNFLSVYIANA
                     ADEMVAFLKQLVWNKETEEEQRKINYAASVYLGDLLVTCYSLYSRNRTFGNMIGKGYS
                     VKAAQLELNMIAEGYNASKCIFKTNQTVNANIPIATIMYKILWEQLPAEIGFKEIEKL
                     LI"
     gene            complement(10342..10842)
                     /locus_tag="PLY81_01185"
     CDS             complement(10342..10842)
                     /locus_tag="PLY81_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNF28873.1"
                     /translation="MLSKTMQEALNRQVLMEAQSSQAYLAMASWAEIQPGLKGVTEFF
                     FKHSDEERMHMLKLVHYINERGGFAVIPSLEQPVLTFVSLKKAFEELLKHELAVSNSI
                     NELVDISLKEKDYATHNFLQWYVTEQMEEERLARECNDKLEMIGDDKSGLYLFDRDIM
                     SMENGG"
     gene            11016..>12950
                     /locus_tag="PLY81_01190"
     CDS             11016..>12950
                     /locus_tag="PLY81_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF28874.1"
                     /translation="MLKFKPLFLLLLLFNFANFKIFAQQDTIKGVGSEVVVTGQYSPQ
                     PLRSSVYKVKTITAERIKMRASTDIVGALNSELGIRFSTDNSLGETDTKILGLGGTRV
                     KILIDGVPMADRDATKQSINQIDINSIEKIEIVEGPMSVIYGTDALAGVINIITKKGS
                     KNGNHLSVGLKIQEEGIGNTYVPFTKNGIHNQNLSVNWNNNKYRASAYLTRNDFGGFA
                     DTAAYPAKVFRPKKQWMTGGTVGYKNSIFDIWYRVDFLHEELLSASPMSLTTYKSFRQ
                     YYYTKRYTHQLQSIWNINKELKLNSAVSFQDYNRTTESYTLDYRTGINSPNKTKQDIE
                     SGYWDVTPFQSLFVRTALSWIVSSKVSLQPGLEFKQDKTSGERVSGSPSISDFSFFIS
                     SEIKPTSKINIRPGLRLSKNSVYDAPPIIPSINTKFTLNKLLDLRLSYARGFRAPILR
                     ELYFNFYDANHNIKGNTNLKAETSNSFMTTITCNAITKNHFSYASTITGFYNSYKDFI
                     DLYDYIDNNGVATFSYFNRDNFKTKGITFENNLSYKNLVAGIGLSYIGYYNRFQENNT
                     LQGNTAKFAWSPEISTNITYHLKKLKGSIGMYYKFTGQIPTFSLGTNNDIVLSKRNAF
                     HWADLTATKQLFKLITLQAGI"
BASE COUNT         4756 a         1898 c         1964 g         4332 t
ORIGIN      
        1 acacgaaaat tttgttttgc ctgttgcaat tatttgtaat taattggttt aacaatttat
       61 ataatagcta aaactatttt ttatttaaaa gccttttttc tacagcctcc caattaataa
      121 tattccacca attattaata tactcagtac gtttgttttg gtattttaaa taataggcat
      181 gctcccaaac atctaaacct aaaatagggt atcctttcac ttctgcaaca tccattaatg
      241 ggttatcttg attgggtgta gagctaattt ttaaatggcc gtcgttttca attaaccatg
      301 cccaaccgct accaaaacgt gtagccgctg cttgtgaaaa aagtttttta aactcctcaa
      361 aactgccaaa agaattgtta attacttcta gtactttttg tgttggcttg cctgatgatt
      421 taggtgtaag cgtttgccaa aataattcat gattataatg cccaccagca ttgtttctca
      481 ttttagagga gtaagaagaa attttagcta aaatatcttc tatagtaatt gcattagatt
      541 tcttgtattc atctttcaaa gcctcattca ggtttttggt ataacctgca gcgtgttttg
      601 tataatgaat ttccattgta gcagcatcaa tatatggttc caaatcttta taactatact
      661 gcaagggttg ttgtttaaaa tttactacta caccatcttc tttggcttgt aatattgttg
      721 gaagaatggt taaaccaata ccaacttttg ctgtatttgt aataaaattt cttctattta
      781 ttttgttatt tttcatacac taaactttta tttttttaaa ttttttaata aattcacgta
      841 ctcccttaaa tgttctataa taaaactcct ggttaaacaa ttgagagtct ctcattgctt
      901 taaaggtaag aaaaaagcca atgggcaaca gtatcattga acttaaccac ataccaacaa
      961 aaggagatgc tacagactct tccacaaatt tttcgccaaa agtgtttatc atgtgaaaaa
     1021 tgataaagaa aataatagaa aatactaatg gtaagcctaa gccacctttt cttattatgg
     1081 agcccaaagg tgcaccaatt aaaaataaaa caatacaagc aaaacttaaa gtaaactttc
     1141 tatgccattc tatttgatga ttaattttgg attcattttt aaattcaaaa tcgttagctg
     1201 ctatagcagt tgtgttgcga gctatattta atttactatt agcattatca aaaataatag
     1261 ttcttatact atctggtatt atagcagtaa tattgtttac atgctttaat ggtttagcct
     1321 gtttgaaagt agcatttaca tattgcacaa atggtagggt ttctgctact tctgtattat
     1381 ttcttttaat aaatttttgt tgaattgttt taaaagaatc tattgaataa ttaagttgcc
     1441 taatgcttag cattttagga tcatacctaa aactagagtc ggcagattgg ttcatttgca
     1501 acgatcttaa atcgaatatc tttttatatt ctttaaatcc aagtctgata taatcggtat
     1561 ttgtagcact atttgcttgt gctttttctt catatcgcca accatctttt aaaacaaatt
     1621 ctaaaaattt tttgttgtta gaaaccttca ttataccact atctgcaaca ataatattat
     1681 cttgtatgcc atacctcttt tcaaaaatta caatattttt tatagtacta tcatcttttt
     1741 ctttcttacc aatttttatt gcaaaatctt ttatttggtt ataaaataca ccttctttta
     1801 tatcaaaagc aggctttgca attatgatat catattttaa agctgctaat tttaattgag
     1861 caacaggtat tacattattt gcaaaaacaa atgccaagcc actaattgct atagccacaa
     1921 ttagtaaggg cctcataaat cttaataaag aaatacccga tgattttatt gcaactaatt
     1981 caaaggtttc gcctaagttg ccaaatgtca ttatggatga aagtaacata gccaatggta
     2041 aagccaatgg aactaaagtt gcagtaacat aagcaattaa tttaaaaatg gttgtaactt
     2101 ctaatccttt gcctactaaa tcatcaatat aaagccaaaa gaattgcatt actaatacaa
     2161 acaacgaaat aaaaaaagta gcaaagaaag gggctacaaa agatcggagt attaatgtat
     2221 ctaatttttt aaacactatt gcttttctaa tttttattca atgtagcaaa tgtacttatt
     2281 tatgtagcag aaactctata acaataatgt acaaaagtgt aaaaaagcta gaaaaaattt
     2341 gtgaagtgtg agagaatctt tttagtgatt aatttgtaga agagatagtt tttaacttcc
     2401 taatctatgt aataattctt ttacctgata atcccatagc tgcgtttgat cttttatttc
     2461 ggacttttcg gcaaaatctg aaattactaa aatagtttgg tttgaaattt ctgttttggc
     2521 aattttaaat tcaaaatatt cttcttttgg tgcatgtaac catcggtatc taataaattt
     2581 atcttgctct gcatctatta caactgcctt atctggtgtg ccaccactcc aactaaaact
     2641 caattcccct tcccactcat ctactttatc tgcaaaccat tcttgcaaac cggttggagt
     2701 gcttaagaat tcaaataaaa ttgatggtga acatctaact ttaaactcaa gtgtaaatag
     2761 ttgtttttta ctcattagta agtctttaaa cctttatcaa gtgctgcaat aatagcaatt
     2821 aatacagttt aaacaaatgt ttttataatt ttattttaat tctttattca gaattctata
     2881 taaaaaaata attcatacaa tttaggttaa gtaaataatt gataattaag atgaaagcta
     2941 attagtacaa tttattaaaa gataaaaagg tatttagggc aacaacttta gtgctattat
     3001 atttgcagcc ttattttaca tcaaagagta ataaaaataa aatataatgt ttaataattt
     3061 atctgaaaaa ttagaaatag catttaaaaa tcttaaaggc cagtctagaa taaatgattt
     3121 aaatatagcc aatactatta aagatattag gcgtgcctta ttagatgcag atgtgaattt
     3181 taaaattgcc aaagaattta cagataaagt aaaggtagaa gctattggta gtaaagtgct
     3241 ccatgctgtt agtcctgggc aacaaatggt aaaaattgtt caagatgaat taactgcatt
     3301 aatggggggc gaacctgcag agtttaatat tagtggtaac cctgctatta ttttaatagc
     3361 tggcttacaa ggtagtggta aaacaacttt tacaggaaaa ctggctactt atttaaaaga
     3421 taaaaaagga aagtcgccaa tggtggttgc tgcagatatt tatagacctg ctgctataga
     3481 ccagttaact gtactagcac aacaaattgg tgtagatatt tataaagaac ccgaaacaaa
     3541 agatgcagtt ttaattgcac aaaatgcaat taaagaagca aaggcaaaaa ataaaaatgt
     3601 tgttataata gatactgcag gtaggcttgc aatagatgaa ataatgatga ccgaagttgc
     3661 caatattaaa gcagctgtaa acccttcaga aattttattt gttgtagata gcatgacagg
     3721 gcaagatgct gtaaacactg caaaagcttt taatgataaa ctagatttta gtggtgtggt
     3781 tttaactaaa ttagatggtg ataccagagg tggtgctgct ctttctataa aatacacagt
     3841 aaataaaccc ataaagttta tgagcagtgg agaaaagatg gatactttag atgttttcta
     3901 tccagataga atggctcagc gtattttagg aatgggagat attaccacct tagtagaaaa
     3961 agcacaacaa caatttgatg aagagcaagc taaaaaatta gagaaaaaaa taagaaaaaa
     4021 tcaattcgat tttcaagatt ttttagagca gctacagcaa ataaaaaaaa tgggtaatgt
     4081 aaaagactta atgggtatga tacctggtgt tggtaaagca ataaaagatg ttgaggttga
     4141 caacagcagt tttaaaggta ttgaagccat tattcaaagt atgacacttg aagaaagggc
     4201 taaccctgat ttaattaacc caacccgaaa aagcagaatt gcaaaaggtt gcggaaaaga
     4261 tattgccgaa attaatgctt ttataaagca gtttgaacaa atgaaaaaaa tgatgagtat
     4321 gatgaataaa atgccaatgg gtggtttatc aaatatgatg aaaggaatga aacgctagag
     4381 aataaaattt ttcaatatat tttagccaag tttatgtaaa ctattaatta actaaaatta
     4441 ttttcaaaat aaaaagcctt gtaaaaaaaa ttacaaggct tttttagtgt acaaattata
     4501 gttatggagc cactaatcta aatccattgc catgaatatt aactatttca attttttcat
     4561 cttcttttaa atacttacga agtttagcaa tatatacatc catacttcta ccattaaaat
     4621 aagtatcact accccaaatt ttcttcaatg ctctttctct tggtaataaa tcatttttat
     4681 gctcggcaag catttttaat aaatcatttt cttttggcga taaagtttga gtgctccctt
     4741 ctctaatcaa ttctcttaat ttaggattaa aatgataggt acctaaatca aactctatat
     4801 tttcgtttac ctttgtttcc tcttcgtttc tttttaaaat tgcttttatt ttatgtaata
     4861 aaacttcact gtcaaatggt ttggtaatat aatcatcggc tcctaattta tagccttgta
     4921 ttatatcttc tttcatagtt ttggcactta aaaaaaataa aggaatatcc ggatctatat
     4981 ctctaatttc ttcagcagca gtaaatccat ccatattggg catcataaca tccaataaac
     5041 atatatcaaa tttttctcgc tgaaatgcag ctaaaccaag ccttccatct ctttctagta
     5101 ctacatcata atcgttcaac tctaaataat tttttaaaac catgccaaga tttgtgtcat
     5161 cttcgcaaag cagaattctg tattttcttc cttccataat gttaatgatt tgtattaaag
     5221 atataaatga attgtaaaag tcataccact tattcaaaag gttttgttaa agaagtaaaa
     5281 aacaggcaaa tatttgtatt ttgggcatct caaagtaaat aaatacttat agtgggcatt
     5341 gaaattatta ctaccctaat ataaactata aactaataaa taaaatttga tagtatcttc
     5401 attttatttt tttaacaact tatgaatata aaaagaaaaa ttattaacga ccctgtttat
     5461 ggttttatca caatagatca tcctatatta tttaaaataa ttgctcaccc atattatcaa
     5521 aggctaagaa gaatacatca aatggcattg gcttatttag tatatcctgg tgcagttcat
     5581 acaaggcttc atcacagttt aggggcatac catttaatgt gtttagcaat aacagaatta
     5641 aaggcaaagg gaacagaaat tacagaagag gaagaaattg cagtaaaggc agctatatta
     5701 ttgcatgata ttggccatgg agctttttct catgcattag aaaaacaaat tttaaataac
     5761 atacatcacg aagagttgag ccttaaaatg atgaaagtta ttaatagtga attaaacggg
     5821 caattagata ctacaatagc catttttact aataactatc ctaaaaaatt tttacaccaa
     5881 ttagttagcg ggcaattaga tgtagataga atggattatt taaccagaga cagttttttt
     5941 acaggtgtaa gtgaaggggt aattggttac gatagaatta ttaaaatgct tattgtacat
     6001 aatggtgagc taatggtaga agaaaagggt atttatagta ttgaaaaatt tttagtagcc
     6061 agaaggcaaa tgtattggca agtttattta cataaaactg tagtggctgc cgaagttatg
     6121 ttggtaaata ttttaaaaag agttaacgaa ttaagcgtaa aagataaaac agggctacaa
     6181 acagatggtg ctttagatta ttttttattt gaatacacta atggtttaaa tttacaagta
     6241 gtagaaaaat ttagcgaatt agacgatagt gatttaagtt ttgccataaa aaaatggagt
     6301 aaacacaagg attttgtttt gagcaccctt tgtaaaaaac tattagccag aaacttatat
     6361 aaaataaaac ttcaaagtaa accttttaat tctgaagaaa taaacgaatt acaagtaaaa
     6421 gcaatgcagc aatttaaggt agatagtagt ctaattcctt attttgtaca tactggtact
     6481 gccaataata caatgtataa aacagaagat gaaagaataa atatattata taaagatgga
     6541 acagttaaag acatttcagc aatagataac ccattaatac atcaaactat atcggcacca
     6601 ataaaaaaat tttacatttg tttacttgaa ccctaaaaag tatatttact atacagcata
     6661 attataaaat gtttacagca caacaaatag cattattagt taatgcaaaa atagaaggag
     6721 acccaaatat tattgtttca tcttttggaa aaatagaaga agctaaagaa gggcagttga
     6781 cattttttgc caaccctaaa tacgaagatt ttttatatac taccaccgcc tcagttgttt
     6841 tactaaacga aaactttgtt ctaaaactac ctattaaagc aaccttgctt agagtagcag
     6901 atgcttactc tgcttttgca attttattaa acaaatacca agcaatacaa gcacaacaat
     6961 tagaaggcat tcaacaacct gtttatattt cgccatcggc aaaaattgat gaaaaagttt
     7021 ttatagcagc ttttacttat ataggagaaa atgtgaagat tggaaaaaat gtaaaactat
     7081 ttgctggcgt atctcttggt aataatgttg aaatagacga taacaccatt ttacatgcag
     7141 gagtaaaaat atacgaaggt tgtaaaattg gaaaaaatgt aattattcat gcaggaacag
     7201 taattggtgg agatggtttt ggttttgccc cacaagccaa tggtagttat caaaaaatac
     7261 ctcaaatagg aaacgtaatt attgaagatt ttgttgaaat tggagcaaac acaacaattg
     7321 accgtgcaac aattggtagc acaataataa gaacaggtgt taagttagat aacttaatac
     7381 aaattgccca caatgtagag gttggtaaca atacagtaat tgcagcacaa gcaggggtta
     7441 gtggaagtac aaaaattggt aaaaatgtaa tgattggtgg acaagcagga attgttggcc
     7501 atattactgt agcagatggt agcaaaataa atggacaaag tggtgtaagc aaaagtatta
     7561 ctgaacccaa ccaagctgtt acaggctcac cagcttatga ttatactgct gcattaagaa
     7621 gccaggcagt tgtaagaaaa ttacctgaac tagaaaaaag aataaaagaa ttagaaaatc
     7681 aattatcgca aattttagct ttagaatcaa agtcttaaca tatttctatt attctattac
     7741 ttctatttaa tgaatacatt tctaaaatta tctgatgaag ttgcgaatgc tttacaacaa
     7801 aaaaaacctg ttgttgcttt agaaagtacc attattgctc atggtatgcc ctacccaaaa
     7861 aatgtagaaa cagcattagc tgttgaacaa acaataaaaa ataatggtgc cacacccgct
     7921 acaattgcaa ttattaatgg aaaatgctgt gtaggcttaa ccgctgaaga aatagaaatt
     7981 tttgggaaag aaaaagaagt atggaaagtg agtttaagag atatgcctta tgtaatttct
     8041 aaaaaattat ttggggcaac tactgtagct gctacaatgc gtattgcagc aatggcaggt
     8101 attaaaattt ttgtaacagg aggtattgga ggtgtgcata gaggtgctga aaaaactatg
     8161 gatatcagtg cagatctttc agaaatggca gaaacaaatg ttgctgttgt atgtgctggt
     8221 gtaaaatcta ttttagatat tggcttaact ttagaatact tagaaaccaa aggaattcct
     8281 gtagtaactt atggccaaaa atcttttcca agtttttatt caaccgaaag taattataac
     8341 actccattgc aattaaactc atctaaagaa attgcagaaa tgctacaaac caaatgggca
     8401 ttgggtttaa atggttcagt acttattgcc aacccaatta ataaaaaaga tgaagtgcct
     8461 tttgcagtta tagaaagcca cattactaat gctttggaac atgctgaaaa acaaggaata
     8521 aaaggaaaag aaactacccc ttttttatta caacatattg ctcaacatac taatggcaat
     8581 agtttagagg caaatatttc tttaataaaa aataatgctg tacttggtgc tcaaattgct
     8641 gctcatttat aattatttgt ataatgaaca ttttttctcc gtataaaata aattcatttg
     8701 taatgggtag taatattaat attgaactag ctaattggag aaaaaatatt aattatataa
     8761 cacttagaat ttttcctttt gtattatctg ttataactgt ttatgctgtt atattaaatt
     8821 ttaaggaaat gccaaaagca tattcttatg tattattaat tggtattcca gcactttctg
     8881 tttatatgtt ctttactaaa tattctgttc aaaccatcat taaacctcca tacatacaag
     8941 tgtataaaaa ttctttcaga ggcaaagaag aatcaaatat taattttaat agcattcata
     9001 aattaacctg taaaataagg tatggcaaat acggaggaac ttttttttac ttgcatacct
     9061 cagccaataa aaagatagag tttataacta ttccggttac aaacatgaag aaagaatata
     9121 cagatttaat tattaaagaa ctagaggcta ttgtaggcaa aacagttgaa tttgtttaag
     9181 ctacattata aaaaacttac aaacttcaaa acattatctt tgttaggcac taatacaatt
     9241 gtactatgaa atttggagtt attggtagcg gaagttgggc tactgcactt gcaaaaattt
     9301 taaccgataa tggtaatcac attaattggt ggattagaaa cacagatacc atttcctatt
     9361 tacatacaag aaaacataat ccacgttatg ttagtagtgc ttattttaat acccagcaac
     9421 tttatatgag taatgaaatt actgaagttg ttgaaaaaag taatgttttg gttattgcta
     9481 ccccttctgc ttttacagaa gatgtgttga aacaattacc aagtaatgct tttgagggga
     9541 aaataattat ttctgccata aaaggtattc taccttcaga aaatgattta ttaaatgaat
     9601 atttaaaaaa acattttaat gtacctatag aaaattattg taccatactt ggcccctgcc
     9661 atgcagaaga agtagcttcg gagaaactaa gctatttaac aatttcaaat attaatgaaa
     9721 gccttgcact tagtatagca aaacatttta atactgaata cttaaacaca agagtaaatc
     9781 acgatatttc aggcgttcaa tatgctgcaa tattaaaaaa catttatgct ttaggtgcag
     9841 gtattgcaca tggtttagaa tatggagata attttttaag tgtttatatt gcaaatgctg
     9901 cagatgaaat ggttgctttt ttaaaacaac tggtttggaa taaagaaact gaagaagaac
     9961 aaagaaaaat taattatgca gcatctgttt atttgggaga tttattggta acctgttatt
    10021 ctttatatag cagaaacaga acttttggaa acatgattgg aaaaggatat agtgtaaaag
    10081 cagcacaatt agagcttaat atgattgctg agggatataa tgcaagtaag tgtattttca
    10141 aaaccaacca aaccgttaac gcaaatattc caattgctac aataatgtat aaaattttat
    10201 gggagcaatt accagcagaa attggattta aggaaattga aaaattatta atttaaaaaa
    10261 gccgttatac taataacggc tagtaaaaaa taaactattc tataagttaa gcaagtgaat
    10321 acttacattc agaaattgtt tctaacctcc gttttccata ctcattatat ctctatcaaa
    10381 taaatataaa ccacttttat catcaccaat catttctaat ttatcgttac attctctggc
    10441 aagtctttct tcttccattt gttcggttac ataccactgc aaaaaattat gtgttgcata
    10501 atctttttct tttaaagaaa tatctactaa ctcatttatg ctattgctta cagccaactc
    10561 atgcttcaat aactcttcaa atgctttttt caatgacaca aaagttagca caggttgttc
    10621 taaagaaggt attactgcaa agccacctct ttcattaata tagtgtacta attttagcat
    10681 gtgcattctt tcctcatcac tatgtttaaa gaaaaattca gttacccctt ttaatccagg
    10741 ctgaatttct gcccaactag ccattgctaa ataagcttga gaagattgag cttccattag
    10801 cacttgccta tttagtgctt cttgcattgt tttagataac atactttttg ttttactcaa
    10861 agttaaaaaa atacattatt aatataacaa catactggtt tttattttta aaaaaagaat
    10921 attaactgat taacatttga caattctatg gcagatttta aatctatatc attgaaaatt
    10981 tgcagatatt taaaaaactg ctttaaactt actatatgtt aaaatttaaa cccctctttt
    11041 tactactgct cttatttaat tttgccaact ttaaaatttt tgcacagcaa gatactatta
    11101 aaggagtagg cagcgaagtg gtagttacag gtcaatactc tcctcaacca ttaagaagtt
    11161 ctgtttataa agttaaaaca attactgcag aaagaattaa aatgagagct tctacagata
    11221 ttgttggtgc attaaacagt gaattaggaa taagattttc taccgataat tctttaggag
    11281 aaacagatac aaaaatttta ggcttaggtg gcacacgtgt aaaaatttta attgatggtg
    11341 ttccaatggc agatagagat gcaaccaaac aaagtattaa tcaaattgat ataaactcaa
    11401 tagaaaaaat agaaattgta gaaggaccaa tgagtgtaat ttatggtacc gatgcacttg
    11461 caggtgttat aaacattatt acaaaaaaag gttctaaaaa cggaaaccat ttgtcggttg
    11521 gtttgaaaat tcaggaagaa ggcattggta acacctatgt accttttacc aaaaatggta
    11581 ttcacaacca aaatttatca gttaactgga acaataataa atacagagct tcggcttatt
    11641 taaccagaaa tgattttggt gggtttgctg atacggctgc ttatcctgca aaagttttta
    11701 gacctaaaaa acaatggatg acaggtggta cagtaggtta taaaaatagc atttttgata
    11761 tttggtatag agtagatttt ttacatgaag aattgctttc tgcaagccca atgagcctta
    11821 caacttataa aagttttaga caatactact atactaaaag atatacccat caattacaat
    11881 ctatttggaa tataaacaaa gaattaaaat taaattctgc tgtttctttt caagattata
    11941 atagaacaac agaatcttat acgcttgatt acagaacagg aattaactct cctaataaaa
    12001 caaaacaaga tattgaaagt ggctattggg atgttacccc ctttcaatct ttatttgtcc
    12061 gtactgcatt atcttggata gtttcttcaa aagtttcatt acaaccaggg ttagagttta
    12121 aacaagataa aacaagtgga gaaagggttt ctggctctcc ttctatttct gatttttcat
    12181 tttttatatc ctcagaaata aaacctacat caaaaattaa tatacgccct gggttacgat
    12241 taagtaaaaa ttctgtttac gatgcaccac ccattattcc ttctatcaat acaaaattta
    12301 cactaaataa attgttagac ttacgcttgt cttatgcaag aggttttagg gccccaattt
    12361 taagagaatt atattttaat ttttacgatg ctaaccataa tataaaagga aataccaact
    12421 taaaagccga aacatctaac agctttatga caaccattac gtgtaatgca attaccaaaa
    12481 atcatttttc ttacgcatca accataacag gtttttataa cagctataaa gattttattg
    12541 atttatatga ttacatagat aacaatggtg ttgcaacttt ctcttacttt aatagagata
    12601 attttaaaac aaaaggaata acttttgaaa ataatttaag ctataaaaat ttagttgcag
    12661 gtattgggct ttcatatatt ggatactata ataggtttca agaaaacaat accctacaag
    12721 gcaatacagc taagtttgca tggtcgcctg agatttctac taacattact taccatttaa
    12781 agaaacttaa aggtagtatt ggcatgtatt ataaatttac gggtcaaatt cctactttct
    12841 cattaggcac taacaatgat attgttttat caaaaagaaa tgcatttcat tgggccgatt
    12901 taacagctac aaagcaatta tttaaactta ttaccttaca agctggcatt
//
LOCUS       DAOQHP010000017        12226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2538_length_12226_cov_3.633144, whole genome shotgun sequence.
ACCESSION   DAOQHP010000017 DAOQHP010000000
VERSION     DAOQHP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_12226_cov_3.633144"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..850
                     /locus_tag="PLY81_01195"
     CDS             314..850
                     /locus_tag="PLY81_01195"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNF28875.1"
                     /translation="MSKTNNIVFWALYAIAWIIFIGLSIEAGGLIVNFLFSLYKPEIV
                     QNLYQKLDLIQMYNENKFAFFSIYSFILIISILKAVLFYTVISLMHTMDRTKPFSTFV
                     AKKISYISYFTLSIGLLSFIGREVTKNLMHEGLALNKVNQFWVDSEAYIFMWAVIHII
                     ATIFKKGVAIQNENDLTV"
     gene            854..1078
                     /locus_tag="PLY81_01200"
     CDS             854..1078
                     /locus_tag="PLY81_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF28876.1"
                     /translation="MPIIVNLDVMMAKRKISLNELSEKVDLTLSNLSILKTGKAKAIR
                     FSTLDAICKALNCQPADILEYKSEEENTGN"
     gene            complement(1278..1718)
                     /locus_tag="PLY81_01205"
     CDS             complement(1278..1718)
                     /locus_tag="PLY81_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF28877.1"
                     /translation="MATVNVYLTFNGNCEEAFLFYQSVFGGEFPYIGRFNEMPQNDPN
                     CPPMSEEDGKKIMHVTLPISKETCLMGSDTFGDFSPPHIQGNNFSISINTNSTAEADN
                     LFNALSKNGQVIMPLNKTFWGAYFGMFTDKFGINWMVNYDEQTA"
     gene            complement(1746..2183)
                     /locus_tag="PLY81_01210"
     CDS             complement(1746..2183)
                     /locus_tag="PLY81_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28878.1"
                     /translation="MKTLEYNIQLNQPSEKVWDCLWQIDNYKKWTRPFSEGTYYKTSD
                     FKQGNKIHFLLPDGNGMYSTIEVLIINKLVIFNHIGEIKNFEEQPLDEKTKQWTNAKE
                     SYELVETTYGTNLNIKVDCADEYIEYMNTAFLLAIEEIKKLAS"
     gene            complement(2269..2661)
                     /locus_tag="PLY81_01215"
     CDS             complement(2269..2661)
                     /locus_tag="PLY81_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28879.1"
                     /translation="MLKKKFVKIFLLVFILILIIFGYFRFFYVFAEGTKAGVLNTFQK
                     KGYVFKTWEGIIIQSGFKQNVQSNEFEFSVINEKIAKELEKHSGKEVNLHYNRYFGTL
                     PWRGVSVYVVDSIFEIRNPDNKTNITPQ"
     gene            complement(2674..4035)
                     /locus_tag="PLY81_01220"
     CDS             complement(2674..4035)
                     /locus_tag="PLY81_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF28880.1"
                     /translation="MKKILLLLLLPIVANSQQTDSLFIKKIADEILTNGKAYDLLYHL
                     TKKIGGRLAGSPQYNKAVNWGFNTMQQMGADKVFLQECIVPHWVRGVGDFATLQNTNS
                     SSVKKQLNITAIGNSLGNTKIIKAPIILIHNFQELENKKEEVKGKIVFYNAAFNPTNI
                     RTFKSYGETASYRVNGASRAAKYGAVAVIVRSLTEATDNHPHTGTLRYNDSFPKIPAV
                     CVGLKDADYIAQNIKKGNWQVALKTNGKLLPDTINHNVIAELKGTEFPNEYITIGGHL
                     DSWDVSEGAHDDGAGVVQTIEILRAFKALGYQPKHTIRFVLFANEENGTKGGLKYAEK
                     AKENKEQHIFALESDAGGFTPRGFLFTMDKEKLAKVKLWLPLLKPYGTEALDAGGGGA
                     DIGPLNKTFNTPIAGFMPDTQRYFDVHHSVNDIFENVNKRELLLGAVNMAALIYLIDQ
                     HSL"
     gene            complement(4113..5237)
                     /locus_tag="PLY81_01225"
     CDS             complement(4113..5237)
                     /locus_tag="PLY81_01225"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNF28881.1"
                     /translation="MRLLLMLAILIVFFTTVTAQKVFKDANGSYGLKDNNGKIILKPT
                     YTFIRSFVDGFAAIAINKYPNIKWSFINTKGQIITPFKYDKIDFFSEGLAVVKLNKLY
                     SYINSLGKEVFGFIFLEAFGFHQNIAVVKNKNGKYGVINKQGKTVVNYKYDNMNSHFT
                     EGLAWVTLNNKVGFINAKGIEVAACIYDDAGNFNEGMAPVKQNGKWGFINNKGKVVIP
                     FQYDVTSLNSFNNGLVKVKINEYYGFINKQGEEVIPFIYENVDDFSEGLAGFLKEGKY
                     GFMDLTGKEIIKPIYKYTWGFNNGLAAVEVENEKKENKWGFINKKGILVIPALYSSIL
                     PQQNKHTHKIIFGFYKNKAYVSLSDKNNFYINNKGLKISD"
     gene            complement(5359..6552)
                     /locus_tag="PLY81_01230"
     CDS             complement(5359..6552)
                     /locus_tag="PLY81_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF28882.1"
                     /translation="MSNTLQSNESISSSRKKIIVLGSGPNRIGQGIEFDYCCVHGLLA
                     IKECGYEAIMINCNPETVSTDFDMADKLYFEPVYWEHLWEIIELEKPEGVIVQLGGQT
                     ALKLAKKLHEKGIKIIGTSYNDMDIAEDRGRFSDLLKELEIPYPNYGTAYTAEEAIEV
                     ANKVGYPVLVRPSYVLGGQRMRIVINDEEVEKSCISILKHIPDNKILIDHFLDRCQEA
                     EVDAIYDGKSFHVMGIMEHIEPAGIHSGDSNAVLPAFNLTPLVIETMQFYSEKIAKAL
                     NIKGLINIQFAIKDGKVYVIEANPRASRTTPFIAKAYQIPYLNIATKVMLDKAKLSDF
                     NLEKKLDGYAIKEPVFSFNKFPGVNKELGPEMKSTGEAIRFIKDLRDPYFRTLYKERS
                     MHLSK"
     gene            complement(6686..7885)
                     /locus_tag="PLY81_01235"
     CDS             complement(6686..7885)
                     /locus_tag="PLY81_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28883.1"
                     /translation="MTFNDVIGNKNVKQHLLELMLQNRLPHALLFLGKEGSGALPLAL
                     AFAQLIICQPKSSPVVEDLFGGMSAISNEPSFLHPNDIESSTSYSKANLLVHPDLHFS
                     FPFINKKNSDSSTISADYITEWREFLMQSPYGNLYDWLQVIKAENRQGNISAKECLEI
                     IKKLSLKSFESDYKVLLMWLPELLSKEGNKLLKLIEEPPPNTIFIFVAENEEKILPTI
                     LSRCQLIKIPLPENDEVEEALLKKANIEPEQAKQIAAISNGNYREALHLIQHAEEDWQ
                     GLLRDWLNTIMRRLLPENIKWIEEISKIGREKQKQLLQYFNHTIEQALRLKTIGADNI
                     SLSESEKDFAQRFNKIANISQMQAISEELDKATFSIERNVNGKIVFTALSVKLYHILI
                     NKVVVEV"
     gene            complement(7982..8260)
                     /locus_tag="PLY81_01240"
     CDS             complement(7982..8260)
                     /locus_tag="PLY81_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNF28884.1"
                     /translation="MKEHKSHTIDTASSTKKITSLFGKENYILMAVGAIIITVGMLLM
                     SGGKNQDPNVFDESVVYSTTRITVAPILILIGFVVEIYAIFKKPKASA"
     gene            complement(8329..8805)
                     /locus_tag="PLY81_01245"
     CDS             complement(8329..8805)
                     /locus_tag="PLY81_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF28885.1"
                     /translation="MDKNIETIQKFYTAFQQKDYETMNSCYANDIAFSDPIFGLLHGN
                     QVKAMWQMLCTNAKEFKLQFNNIKTDDDEYYTCSWIAMYRFSATNRRVVNKCTAYMRL
                     KDGVIIEHSDGFNYYKWCRQAFGIKGILLGWSGFMHKKVSNKARKQLFKYMEQHGL"
     gene            complement(8811..9458)
                     /locus_tag="PLY81_01250"
     CDS             complement(8811..9458)
                     /locus_tag="PLY81_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNF28886.1"
                     /translation="MNLYYCYDAYCGWCYGFSPIIKKIAEEYKNVLHTEVLSGGMILP
                     ETPTHIGTMANYILKAYKTVEEYAGIKFGKDFLWHIENPTLSDWFPDSLKPAIALCIF
                     KEYHANLQIQFASDLQYALNFEGRDLTDDAAYLHLLTKYNIPKNAFYEKMNSEEYIDK
                     AKYEFSLCKQLQVTGYPCVLLQANESKFYLLAKGFTTYEELKKRVENVLNELTAN"
     gene            complement(9534..10568)
                     /locus_tag="PLY81_01255"
     CDS             complement(9534..10568)
                     /locus_tag="PLY81_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNF28887.1"
                     /translation="MTLQQNNKAVATWLLVGAAMIIIQVLLGGLTRLTDSGLSITEWQ
                     PLLGSIPPLNNTDWQIAFNKYKELAQFKFLHSHFNLNDFKFIYFWEWFHRIWARLMGV
                     VFILPFVYFIYKKWINRTMLMPMIILFLLGGLQGFVGWFMVKSGVGTSLVFVDHTRLA
                     IHFIAALVLLVYVVWFALKLIINNNQLINAPKIKRLNILIIALLTIQLIYGAFMAGSR
                     AAYSAITWPSINGFFYPPGMLHKESLWYDLFNNLLAIQFIHRGLAYLLLIVIIIFTLQ
                     LKKINSSKFLNSIKFLPFHLVTVQVILGIFTIINYTTKYKLILSITHQFIGMLLLVSL
                     FFTLFIVRKK"
     gene            complement(10616..11860)
                     /locus_tag="PLY81_01260"
     CDS             complement(10616..11860)
                     /locus_tag="PLY81_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF28888.1"
                     /translation="MKKLFHKEGLSPLKYKEFRLFIAFRFLYIMALRMVSTVVAYELF
                     QITQSSFSIGLVGLSEFIPVFSLALYAGHVIDKSDKRTMLMKGMMLYAICVVGLIVVT
                     HSSMKKHLSVTAMEMCFYTIIFFTGVIRAFVGPSNNAIIFQLVPKSILHFASNLSSST
                     FLTASIVGHASAGFYIAWFGVNTTFVLILFFVIASVFIVSKIIKKPITNTKLDVKTWD
                     SMKEGLLYVFKNKILLGAISLDLFAVLFGGAVALLPEFASKILRVGPIGFGWLNAATD
                     IGSMLIIISLTLFPLRKRQGEIMLYAVACFGICIMLFGLSNFYTVSFILLLLAGCFDG
                     ISVIVRSTILQLTTPDEMRGRVSSVNSMFINSSNELGQLESGIASRLLGLRPSIIFGG
                     SVTLIVVIITWFKAKQLRKMQY"
     gene            complement(12027..>12226)
                     /locus_tag="PLY81_01265"
     CDS             complement(12027..>12226)
                     /locus_tag="PLY81_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28889.1"
                     /translation="ISTKEENNKTFILQSVNGKQIKWKQTSPTMFVKENDTNSTLSYN
                     KEKKTFSYSSSDGINCEWIKL"
BASE COUNT         4119 a         1967 c         1681 g         4459 t
ORIGIN      
        1 ttaaatacaa atataaattt gaaaactaca tacctaaatg acctgagtca tggctttgaa
       61 agttagctct atgtttctaa gtaacaaaac atgcaagcaa tcaatattac tcaacactta
      121 tttctctaac taattgggca ccaattatag ttgcttttgt atttttaata caatgcataa
      181 ttaataatct gatgtaaacc ctaagaaata ataaattttt taatgtaaaa tattttttga
      241 attaaaatat aaatttattg tttatcaata aatatttatt atgtttgcaa tgtaaaagaa
      301 aaaaatatta aatatgtcaa aaacaaacaa catcgtattt tgggccttgt atgctatagc
      361 ttggataatt tttataggct tatctattga agcaggaggc ttaatagtaa acttcttatt
      421 cagtttgtat aagccagaaa ttgttcaaaa tctctaccaa aagttagact taattcaaat
      481 gtataatgaa aacaagtttg ctttttttag tatttacagt tttattctaa ttatttccat
      541 tttaaaagct gttttatttt acaccgtaat aagcttaatg catacaatgg acagaaccaa
      601 accgtttagc acttttgttg ctaaaaaaat ttcgtacata agttatttta ctctttctat
      661 agggctatta agttttattg gcagagaagt aactaaaaat ttaatgcatg aaggcttggc
      721 tcttaacaag gttaaccaat tttgggttga tagcgaagcc tatattttta tgtgggctgt
      781 aattcatatc attgctacaa ttttcaaaaa gggagtagct attcaaaacg aaaacgattt
      841 aactgtataa attatgccaa tcattgtaaa cttagatgtg atgatggcaa agcgtaaaat
      901 ttctctgaat gaactttcag aaaaagttga tttgacatta tctaaccttt ctatcttaaa
      961 aactggcaag gcaaaagcaa ttcgctttag cacattggat gctatttgta aagctttgaa
     1021 ctgccagcct gcagatattc tagaatataa aagtgaagag gaaaatacag gtaactgata
     1081 atatggtaat acaaaaacaa tagtagattg taatgagcta ttaattaata tttctacaat
     1141 gaaatattaa aattgcaatt gctacctatt taccttgttg tggtaagcca acattaatga
     1201 tacatgttaa tgcaaagcaa taaagaatag aaggcaaaaa atgcaataaa attttatagt
     1261 tgagctactc atgtttacta tgctgtttgc tcatcataat ttaccatcca atttattcca
     1321 aacttatctg taaacatacc aaagtaggca ccccaaaatg ttttatttaa gggcattatt
     1381 acttggccat ttttagatag tgcattaaat aaattatctg cctcggcagt gctgtttgta
     1441 tttattgaaa tggaaaaatt attaccctga atatgtggtg gagaaaaatc gccaaatgta
     1501 tcgctgccca ttaaacaggt ttctttacta ataggaagtg taacatgcat gatttttttt
     1561 ccatcttctt cactcattgg tgggcaatta ggatcgtttt gtggcatttc attaaaacgt
     1621 cctatataag gaaactcacc accaaaaaca gattgataga ataaaaaagc ttcctcacaa
     1681 ttaccattaa aagttaagta aacatttact gttgccattt ttatattttt tattattaat
     1741 aaatattaac tagctaattt ttttatttct tcaatagcta ataaaaaagc agtgttcata
     1801 tattctatgt actcatctgc acaatctacc tttatattaa gattagttcc gtatgtagtt
     1861 tctactagct cataactttc tttagcgttt gtccactgtt ttgttttttc atctaatggt
     1921 tgctcttcaa aattttttat ctctcctata tgattaaaaa taaccagttt gtttattatt
     1981 aaaacttcaa ttgtactgta cataccattt ccatcgggta agaggaaatg aattttgttg
     2041 ccttgtttaa aatcagaagt tttgtagtag gttccttcgg aaaaaggcct tgtccatttt
     2101 ttataattat ctatttgcca aagacaatcc caaacttttt cagaaggttg gttaagctgt
     2161 atattatatt ctaatgtttt caaaatttct actattaaat attattaaaa atagtaaatt
     2221 tttttttatt gctattaaaa aatagttggt agttttttct actaaagttt attgtggtgt
     2281 aatattagtt ttattgtctg ggtttctaat ttcaaatata ctatccacta catatacact
     2341 aacaccacgc catggcaatg ttccaaaata tctattgtaa tgtagattca cttctttgcc
     2401 agaatgtttt tctaattctt ttgcaatttt ttcgttaatt acgctaaact caaattcgtt
     2461 gctttgtaca ttttgtttaa agccgctttg tataattatg ccttcccaag ttttaaatac
     2521 atatcccttt ttttgaaaag tatttaatac accggcttta gtgccttcgg caaatacata
     2581 aaaaaatcta aagtatccaa aaatgattag tattaaaata aatacaagca gaaaaatttt
     2641 tacaaacttt tttttaagca tattcattgt gttttataag ctatgttgat caatcaaata
     2701 aattagtgca gccatattaa cagcacctaa taaaagttct cttttattta cgttctcaaa
     2761 aatatcattt acggaatgat gcacatcaaa atatctttgg gtatctggca taaagccagc
     2821 aattggggtg ttaaatgttt tgtttaaagg gccaatatct gcaccacctc caccagcatc
     2881 taaagcttcg gtaccataag gttttaataa aggcaaccac aatttaactt ttgctaattt
     2941 ttctttatcc atcgtaaata aaaaacctct tggtgtaaaa ccacctgcat cactttctaa
     3001 agcaaaaata tgttgttcct tattttcttt agctttttcg gcatatttca aaccgccctt
     3061 tgttccattt tcttcgttag caaataatac aaatctgata gtatgtttgg gttggtagcc
     3121 taaagcttta aatgctctta atatttctat agtttgcaca acacctgcac catcgtcatg
     3181 tgccccttcg cttacatccc aactatctaa atgtccacca atagtaatat attcattagg
     3241 aaattctgtt ccttttagtt ctgcaattac attatgatta atagtatctg gcaataattt
     3301 tccatttgtt tttagggcta cttgccaatt accttttttt atattttgtg ctatgtaatc
     3361 tgcatctttt aaccctacac atactgctgg tattttagga aaagaatcgt tataacgtaa
     3421 tgttcccgta tgaggatgat tatctgttgc ttcggttaaa cttcttacaa ttacagcaac
     3481 tgcaccatat tttgcagcac ggctagcacc atttacacgg taggatgccg tttctccata
     3541 acttttaaat gttcttatat tggttgggtt aaatgcagca ttataaaaaa caattttgcc
     3601 ttttacttcc tcttttttat tttctaattc ttgaaaattg tggattaaaa taataggagc
     3661 tttgatgatt ttagtattac ctaaagagtt gccaatagcg gtaatattta attgtttttt
     3721 gacagaagag gaattagtat tttgtaatgt tgcaaaatcg ccaacacccc ttacccaatg
     3781 tggtacaata cactcttgca gaaaaacctt atctgcaccc atttgttgca tagtgttaaa
     3841 cccccaattt actgctttgt tgtattgagg cgaacctgca agcctgcctc caattttttt
     3901 tgttaagtgg tataataagt cataagcttt gccattagtt aaaatttcat cagctatttt
     3961 tttaataaat aaagaatcgg tttgctgact attggctaca attggcagaa gcagcagtaa
     4021 taatattttt ttcatacttc taaagatatt aattggatag tacactttag aattaatttg
     4081 atgagaaatt tagactaagg aagggataca acttaatcgc taatttttaa gcctttatta
     4141 ttaatgtaaa agttgttttt atctgataaa gaaacgtagg ctttgttttt ataaaaccca
     4201 aaaattatct tatgggtgtg tttgttttgc tgtggcagaa tagaagagta cagagccgga
     4261 attaccagga ttcctttttt attgataaag ccccatttgt tttctttttt ttcattttca
     4321 acttcaactg ctgcaaggcc attattaaaa ccccaggtat atttataaat tggttttatt
     4381 atttctttac ctgttaaatc cataaatcca tatttccctt cttttaaaaa gcctgcaaga
     4441 ccttcgctaa agtcatctac gttttcgtat ataaaaggta taacttcttc tccttgttta
     4501 ttaataaacc cgtagtattc attaattttt acttttacta atccattatt aaaagagttt
     4561 aaagaggtaa catcgtattg aaaaggtatt actactttgc ctttattatt aataaaaccc
     4621 catttgccat tttgttttac aggagccatg ccttcattaa aattacctgc atcatcataa
     4681 atacaagcag caacttctat accttttgca ttaataaaac ctactttatt atttaaggtt
     4741 acccatgcta aaccttcggt aaaatggcta ttcatattat cgtacttata attaaccact
     4801 gttttacctt gtttattaat tactccatat tttccgtttt tattttttac aacagcaatg
     4861 ttttgatgaa atccaaatgc ttctaaaaaa ataaatccaa atacttcttt acctaagcta
     4921 tttatgtaac tatacaattt gttaagttta acaacagcca atccttccga aaaaaaatca
     4981 attttatcat acttaaaagg agtaattatt tggcctttag tatttataaa actccattta
     5041 atattggggt acttgttaat ggcaatggct gcaaagccat ctacaaaaga acgaatgaag
     5101 gtataagtag gttttaatat tatttttcca ttattatcct ttaacccata gctgccattt
     5161 gcatctttaa aaactttttg agcagttaca gttgtaaaaa aaacaattaa tatggcaagc
     5221 atcaacaata acctcattat aaaaatttaa actaaagtta ttgtttttag gcatattaaa
     5281 gccaatgtgg tcttttttta aagaaattaa atcaaaatag ataaagagat aagtacaatt
     5341 attgttttaa gaagaagatt atttacttaa gtgcatgctt ctttctttat acaaagttct
     5401 aaagtagggg tctctcaaat ctttaataaa cctgattgct tctccagtgc ttttcatttc
     5461 ggggcctagt tctttgttaa cacctggaaa tttattaaag ctaaaaacag gctccttaat
     5521 agcgtagcca tctaattttt tttctaaatt aaagtcgcta agttttgctt tatctagcat
     5581 tactttggtg gcaatattta agtatggtat ttggtaggct tttgcaataa atggtgttgt
     5641 acgagaagct cttgggttgg cttcgataac ataaacttta ccatctttaa ttgcaaactg
     5701 aatattgata aggcctttaa tatttaatgc ttttgctatt ttttcgctat agaactgcat
     5761 ggtttcaata actagtggag ttaaattaaa tgctggtaaa acggcattgc tatcaccact
     5821 atgaatacct gctggttcta tatgttccat aatccccatt acatgaaaac ttttgccatc
     5881 atatatagca tctacttctg cctcttgaca acgatctaaa aaatgatcaa ttaaaatttt
     5941 attgtcaggg atatgtttta aaatactgat acaagatttt tctacttcct catcattaat
     6001 tacaattctc attctttgcc cacctagtac ataacttggg cgaactaaaa caggatagcc
     6061 tactttattg gcaacttcta tagcttcttc tgcagtatag gctgtaccat aattaggata
     6121 aggaatttca agctctttaa gcaaatcgct aaaacgtcct ctgtcttcag caatatccat
     6181 atcattatag gaagtgccta taattttaat tcctttttcg tgtaattttt ttgcaagctt
     6241 taaagcagtt tgcccaccaa gttgtacaat tactccttcg ggtttttcta attctataat
     6301 ttcccataaa tgctcccaat aaacaggctc aaagtataac ttatctgcca tgtcaaaatc
     6361 tgtactcaca gtttcggggt tgcagtttat cattatagct tcgtagccac attctttaat
     6421 ggctaataaa ccatgtacac agcagtaatc aaattcaatt ccttggccaa ttctattagg
     6481 gccactgcca agtacaataa ttttttttct tgaagaggag atagattcgt tgctctgtaa
     6541 tgtgttgctc atttttgtag aaggattgag caaaagtaag gtttgaattt tttaagttaa
     6601 attttttttc ttttgtacat tatattttta ccaatattca tttatttata cagtaattta
     6661 atgatatata aatacttaag tgtttttata cttctaccac tactttattt attagaatat
     6721 gatataattt aacagaaaga gcagtaaaaa ctattttgcc atttacgttt ctttcaattg
     6781 aaaaagttgc tttgtctaac tcttctgata tagcttgcat ttggctaatg ttggcaattt
     6841 tattaaaacg ttgagcaaaa tctttttcac tttcagataa gctaatatta tcagctccaa
     6901 tggtttttag ccttaatgct tgttcaatag tatgattaaa atattgtaat aattgctttt
     6961 gtttttctct gcctattttg cttatttctt caatccattt tatattttcg ggcagcaatc
     7021 ttctcattat agtattaagc caatctctta ataaaccttg ccagtcttcc tctgcatgtt
     7081 gtattaaatg taaagcttct ctataattac cattactaat agctgcaatt tgttttgcct
     7141 gttcgggttc tatatttgcc ttttttaaaa gggcttcttc tacttcgtca ttttctggca
     7201 aaggaatttt tattaattgg catctgctta aaatggttgg taaaattttt tcttcatttt
     7261 ctgcaacaaa aataaaaatg gtattgggtg gtggttcttc aattaatttt aaaagtttat
     7321 taccctcttt agataataat tctggtaacc acattaataa aactttgtaa tcggattcaa
     7381 agctttttag acttagtttt ttaataattt caagacactc ttttgctgaa atattacctt
     7441 gtctgttttc tgcttttata acttgaagcc aatcgtacaa attaccataa ggagattgca
     7501 ttaaaaactc tctccattct gtaatataat ctgcactaat agtagaagag tctgaatttt
     7561 ttttgttaat aaaagggaaa gaaaaatgta aatcggggtg aactaataaa ttagcttttg
     7621 aataactggt tgagctttca atatcgtttg gatgaaggaa agatggttcg ttacttatgg
     7681 cactcatgcc tccaaataaa tcttctacaa caggcgatga ttttggctga caaataatta
     7741 attgtgcaaa ggccaaagcc aaaggtaatg caccgctacc ttctttacct aaaaaaagta
     7801 gtgcatgtgg tagcctgttt tgtagcatta attctaataa atgctgtttt acatttttgt
     7861 tgccaattac atcattaaaa gtcataacta aacgaagata agccttgctt aaaatgaata
     7921 aaaataaaaa gcgatacaaa acattgtatc gctagtaaaa atttgtatat atatctgtta
     7981 tttaagcaga tgctttgggt tttttaaata tagcataaat ttctactaca aagccaatta
     8041 aaataagtat tggtgcaacg gtaattctgg ttgttgagta aacaacactt tcatcaaaaa
     8101 catttggatc ttgatttttg ccaccactca ttaataacat gccaactgta attattatag
     8161 caccaactgc catcaaaata tagttctctt ttccaaataa actagttatt ttttttgttg
     8221 aagatgcagt atcaatagta tgactcttat gttctttcat gcttttaaaa attatgggtg
     8281 caatatatgc aaaactatta tggaaaaggt atttaaactt ttataaaatt ataaaccatg
     8341 ttgctccata tatttaaata attgttttct tgctttattc gaaacttttt tgtgcataaa
     8401 tccgctccag cctaataata tacctttaat gccaaaagcc tgcctgcacc atttataata
     8461 attaaaacca tcgctgtgtt ctataattac tccatctttt aatcgcatgt atgcagtaca
     8521 tttatttaca actctacggt tggttgcaga aaatctatac atagcaatcc aactacaagt
     8581 ataatattca tcatcatcgg ttttaatatt attaaattgt agtttaaact ctttagcatt
     8641 ggtgcaaagc atttgccaca ttgcttttac ctggttgcca tgtaataagc caaaaatagg
     8701 gtcgctaaaa gcaatatcat ttgcataaca actattcata gtttcataat ctttttgctg
     8761 aaaggcagta taaaactttt gaatagtttc tatattttta tccataataa ttagttggca
     8821 gtaagttcat ttaaaacatt ttctactctt ttttttaatt cctcataggt tgtaaaacct
     8881 ttagctaata aataaaattt tgattcattg gcttgtaata aaacgcaagg gtagccggtt
     8941 acttgcaatt gtttgcataa agaaaattcg tatttggctt tgtctatata ttcttcacta
     9001 ttcatttttt cataaaatgc atttttggga atattgtatt ttgttagcaa atgtaaataa
     9061 gcagcatcgt cggttaaatc tcttccttca aaatttaatg catactgcaa atcgctggca
     9121 aattgaattt gtaaattagc atggtattct ttaaaaatac aaagagcaat ggcaggtttt
     9181 aaactatcag gaaaccaatc gctaagtgtc ggattttcta tatgccataa aaaatctttt
     9241 ccaaacttta ttcctgcata ttcttctaca gttttatatg cttttaaaat ataattagcc
     9301 atagtgccaa tatgggttgg tgtttcaggt aaaatcattc caccacttaa tacctctgta
     9361 tgtaaaacat ttttatattc ttctgcaatt ttttttatta taggactaaa gccataacac
     9421 caaccacaat aagcatcata acaataatat aaattcataa aacgaagata aacgtataaa
     9481 actcaaatct aaattaatat atcactttaa ggtagtttca atttaatatt tgtttatttt
     9541 tttctaacaa taaaaagggt aaaaaataaa cttaccaata atagcatacc aataaattga
     9601 tgagtgatgc ttaaaattaa tttgtatttg gtagtataat ttattatggt aaaaattcct
     9661 aaaataacct gtactgttac aagatgaaat ggtaaaaact ttattgaatt taaaaacttg
     9721 ctactattta ttttttttag ttgaagcgta aaaataataa ttacaattaa aagtaaatat
     9781 gccaaaccac gatgtataaa ttgtattgcc aataaattgt tgaacaaatc gtaccataaa
     9841 ctttctttat gtaacatgcc tggtggatag aaaaagccat taatgcttgg ccaggttatt
     9901 gcactataag cagctctaga gcctgccata aatgccccat aaattaattg tatggtaagt
     9961 aaagcaatta ttaaaatatt aagccgtttt atttttggtg cattaataag ttggttgtta
    10021 ttaataatta attttagtgc aaaccaaact acataaacca ataaaaccaa tgctgcaata
    10081 aaatgtattg caagcctagt atgatctaca aaaactaaac ttgtacctac accacttttt
    10141 accataaacc agcctacaaa accctgtaaa ccgcccaata aaaataaaat aatcataggc
    10201 attaacatag ttctgttaat ccatttttta taaataaagt aaacaaatgg aaggataaaa
    10261 actacaccca ttaatcttgc ccaaattcta tgaaaccatt cccagaagta aataaattta
    10321 aaatcgttta aattgaaatg gctgtgtaag aatttaaact gtgctagctc tttatattta
    10381 ttgaaagcaa tttgccaatc tgtattattt aatggaggaa ttgaaccaag caatggctgc
    10441 cattcggtta tagataaacc agagtctgtt aaacgggtta agccacctaa taaaacctga
    10501 ataattatca tggcagcacc cactagcaac caagtggcaa ctgctttatt gttttgttgt
    10561 aacgtcatat taaaattgta aaccgggtaa aaataattaa aaatttactt gtaatttaat
    10621 actgcatttt tcttaattgt tttgctttaa accaagtaat aataacaaca attaaagtaa
    10681 ccgaacctcc aaaaataata gatggcctaa gacctaaaag tctagatgct ataccacttt
    10741 ctaattgccc cagctcgtta ctgctgttta taaacataga gtttacagag gatactctgc
    10801 ctctcatttc atcgggtgtt gttaattgta aaattgtact tcgtacaata acacttatac
    10861 catcaaaaca acctgctaaa agcaataaaa taaaggatac tgtataaaaa ttacttagtc
    10921 caaaaagcat aatacaaatt ccaaaacaag ctactgcata tagcattatt tccccttgcc
    10981 tttttcttaa agggaatagg gttaaggaaa taatgataag catggaacca atgtctgtag
    11041 cagcatttaa ccaaccaaaa cctattggcc ctacacgtaa aattttgctt gcaaactctg
    11101 gtaataaagc aactgcccca ccaaataata cagcaaaaag gtctaatgaa atagcaccca
    11161 ataaaatttt atttttaaaa acgtacaaca agccttcttt catactatcc caggttttaa
    11221 catccaattt tgtattggta attggttttt taataatttt tgaaacaatg aaaacagagg
    11281 ctattacgaa aaaaagtatt aatacaaaag tagtatttac cccaaaccag gcaatgtaaa
    11341 agcctgcact ggcatggccc acaatagaag ctgttaaaaa agtagaggaa cttaagttag
    11401 atgcaaaatg caaaatgctt ttaggaacaa gctgaaaaat aattgcattg tttgaagggc
    11461 ctacaaatgc tctgataact cctgtaaaaa aaataattgt ataaaaacac atttccattg
    11521 cagtaacaga taaatgtttt ttcatggaag aatgagttac aactattaaa ccaaccacac
    11581 aaatagcata cagcatcatg cctttcataa gcatggttct tttgtcgctt ttatcaatta
    11641 catggccagc atataatgcc aatgaaaata caggtataaa ctccgataag ccaacaaggc
    11701 caatagaaaa agagctttga gtaatttgaa atagttcgta agcaacaact gtgctaacca
    11761 ttcttaaagc cattatatat aaaaacctaa aagcaataaa taatctaaat tctttatatt
    11821 ttagtggaga taacccttct ttatggaata atttcttcat tgatttttta gcaatacaaa
    11881 gaacaaattt cagtaaaaga tttgaaagct aacttcttta aaaataaata agttgttttt
    11941 tgaaatgcta aatgaattta tttatacaat ctttttaatt aaaaacagta ttgataggcc
    12001 tgcgtgttga gagtatcttt tatttcttat agctttatcc attcgcaatt aataccatct
    12061 gaactgctat atgaaaatgt tttcttttct ttattatacg ataatgttga gtttgtatca
    12121 ttttctttta caaacatcgt tggagatgtt tgtttccatt ttatttgttt tccatttaca
    12181 ctttgtagaa tgaatgtttt gttgttttct tcttttgtag aaatgc
//
LOCUS       DAOQHP010000018        12190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2550_length_12190_cov_3.932015, whole genome shotgun sequence.
ACCESSION   DAOQHP010000018 DAOQHP010000000
VERSION     DAOQHP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_12190_cov_3.932015"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="rplD"
                     /locus_tag="PLY81_01270"
     CDS             <1..412
                     /gene="rplD"
                     /locus_tag="PLY81_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNF28890.1"
                     /translation="NIRNPLYKGGGTIFGPKPHGYSIKLNRKVKDLAKISALSYKAKE
                     NAILVVEDINLDSPKTKQIAEAMNALNVADKKSILVLGEYNDNVYLSTRNIPNVASTL
                     LADMNTYDIVNADVIVLTENAAKIFSENEVATEA"
     gene            445..744
                     /gene="rplW"
                     /locus_tag="PLY81_01275"
     CDS             445..744
                     /gene="rplW"
                     /locus_tag="PLY81_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNF28891.1"
                     /translation="MKLTEVLVKPILTEKANAQQEKLRRYAFRVNKKANKLEIKKAIE
                     QFYGVTVTNVNTTIAAGKNKTRYTKAGFIEGKKAAYKKAFVTVAEGETIDLYANI"
     gene            791..1630
                     /gene="rplB"
                     /locus_tag="PLY81_01280"
     CDS             791..1630
                     /gene="rplB"
                     /locus_tag="PLY81_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNF28892.1"
                     /translation="MALKKFKPVTAGTRWKIGNAYAEITTNKPERSLLEPVKKTGGRN
                     AQGRRTMRYIGGGHKKHYRIIDFKRNKQNIAATVVSIEYDPNRTAFIALLNYTDGEKR
                     YIIAPQGLQVGATVISGNEAPPEIGNALQMKNMPLGTMIHNIEMQPGQGGKLVRSAGA
                     SAQLTNKEEKYAVLKMPSGELRKVLINCFATVGVVSNSDHSLETAGKAGANRWKGIRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRTGKYAKGEKTRTKGKGSDKLIIQRRDGKK
                     LTK"
     gene            1684..1953
                     /gene="rpsS"
                     /locus_tag="PLY81_01285"
     CDS             1684..1953
                     /gene="rpsS"
                     /locus_tag="PLY81_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNF28893.1"
                     /translation="MARSIKKGPYVDANLEGKVLGMNDGKSKKGVIKTWSRRSTITPD
                     FVGHTFAVHNGNKFIPVYVTEFMVGHKLGEFAPTRHFKGHSGSKQ"
     gene            2042..2383
                     /gene="rplV"
                     /locus_tag="PLY81_01290"
     CDS             2042..2383
                     /gene="rplV"
                     /locus_tag="PLY81_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNF28894.1"
                     /translation="MEAVAKLNNYPTGPRKMRLLADVIRGMEVEKALAILEFHPQHNA
                     MPLAKLLKSAINNWEQKNNGESAANSGLVVKTVFVDGGRVLKRMRPAPQGRGYKIRKR
                     SNHVTIIVDTK"
     gene            2459..3223
                     /gene="rpsC"
                     /locus_tag="PLY81_01295"
     CDS             2459..3223
                     /gene="rpsC"
                     /locus_tag="PLY81_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNF28895.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYGNKKDFAGKLIEDNKIRTYLN
                     ARINKGGIARIVIERTLGKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKEDVQI
                     NILEIRRPELDAQIVGDTIARQIENRINFKRATKMAIASTLRMGAEGIKVKLSGRLGG
                     AEIARSEEFKQGRTPLHTFRMDIDYANVFAQTVYGKIGIKVWICKGEVLGKRDLNPNF
                     ISGKNENAGSDRRGGDRDRRSAGGERGDRRGGRGRN"
     gene            3287..3706
                     /gene="rplP"
                     /locus_tag="PLY81_01300"
     CDS             3287..3706
                     /gene="rplP"
                     /locus_tag="PLY81_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNF28896.1"
                     /translation="MLQPKRSKHRKQQKGRIREVAKRGTSISFGSYALKALEPIWLTN
                     RQIEAARQSMTRAMKREGNVWIRVFPDKIITRKPAEVRMGKGKGNPEFWAAVVEPGRI
                     IFEVDGVSETVAKEAMGLASQKLPIKTKFVVRRDLQA"
     gene            3727..3936
                     /gene="rpmC"
                     /locus_tag="PLY81_01305"
     CDS             3727..3936
                     /gene="rpmC"
                     /locus_tag="PLY81_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNF28897.1"
                     /translation="MATKKFDFNKGIKEITEADLKARITEDSLRLKKLEFAHAISPLE
                     NPMTIRGLRRDIARLKTELKLRTLA"
     gene            4005..4262
                     /gene="rpsQ"
                     /locus_tag="PLY81_01310"
     CDS             4005..4262
                     /gene="rpsQ"
                     /locus_tag="PLY81_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNF28898.1"
                     /translation="MVERNLRKTRVGVVTSNKMTKTVTVAVERKVKHPIYGKFVKKTT
                     KFHAHDEKNECTIGDIVKIMETRPLSKTKRWRLVEVVEKAK"
     gene            4352..4720
                     /gene="rplN"
                     /locus_tag="PLY81_01315"
     CDS             4352..4720
                     /gene="rplN"
                     /locus_tag="PLY81_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNF28899.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIKVLGNSGQDYAHIGDLIVVTVK
                     DAIPAGGIKKGTVSKAVIVRTKNKLRRKDGSYIRFDDNAVVLLNTSNEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            4812..5147
                     /gene="rplX"
                     /locus_tag="PLY81_01320"
     CDS             4812..5147
                     /gene="rplX"
                     /locus_tag="PLY81_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNF28900.1"
                     /translation="MSTRFTPKFNIKKGDTVVVIAGDDKDLKKPRKVLKVIVEKSRVV
                     VEGVNIITKHTKPSAQNTKGGIIKKEAPVHISNVMLWDAKKSAPTKIKRSRESGKLVR
                     ISKKSGEVI"
     gene            5173..5748
                     /gene="rplE"
                     /locus_tag="PLY81_01325"
     CDS             5173..5748
                     /gene="rplE"
                     /locus_tag="PLY81_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNF28901.1"
                     /translation="MSTIKYKPRLADKYKNDVVPALVKKFAYKTVMQTPKLEKICINR
                     GVNGAVNDKKLIDVAIGELEQITGQKAVPTLSKKDISNFKLRKGMPIGAKVTLRGEKM
                     YEFLDRLVSVALPRVRDFKGISDKAFDGRGNYTLGITEQIIFPEIDIDKVNKITGLDI
                     TFVTSAQSNEEAYELLKELGLPFKNAKKDNN"
     gene            5799..6068
                     /gene="rpsN"
                     /locus_tag="PLY81_01330"
     CDS             5799..6068
                     /gene="rpsN"
                     /locus_tag="PLY81_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNF28902.1"
                     /translation="MAKKSIVARQTKRKALVAKFAEKRAALKEAGDYAALDKLPKNAS
                     PVRLKNRCQLTGRPKGYMRYFGISRVMFRDMALNGKIPGVKKASW"
     gene            6137..6535
                     /gene="rpsH"
                     /locus_tag="PLY81_01335"
     CDS             6137..6535
                     /gene="rpsH"
                     /locus_tag="PLY81_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNF28903.1"
                     /translation="MVTDPIADYLTRIRNAQMAGHRIVEIPASNLKKRITEILYEQGY
                     ILRYKFEDDSKQGIIKIALKYDASTKQPAIRNLERVSRPGLRQYAKPTEIKRVINGLG
                     VAIISTSKGVLTDKQAKVQNVGGEVLCYIS"
     gene            6671..7225
                     /gene="rplF"
                     /locus_tag="PLY81_01340"
     CDS             6671..7225
                     /gene="rplF"
                     /locus_tag="PLY81_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNF28904.1"
                     /translation="MSRIGKQPISIPSGVTVSIANGNVVTVKGPKGELKESIDRDIAI
                     EVKDGQILITRPTDQIRHRALHGLYRALINNLVKGVTDGYKKELELVGVGFKAANTGN
                     TLDLALGYSHNIIFEVPKELKVATATEKGKNPIITLEGSDKQLIGQVAAKLRSLRKPE
                     PYKGKGVKFVGEVLRRKAGKSAGK"
     gene            7278..7631
                     /gene="rplR"
                     /locus_tag="PLY81_01345"
     CDS             7278..7631
                     /gene="rplR"
                     /locus_tag="PLY81_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNF28905.1"
                     /translation="MSSNISNKQKIKYRIRKKISGTAVKPRLSVFRSNAEIYAQLIDD
                     NNGVTLTAAASTDKEIAAQKSTKSEKSKLVGQAIARKAIELGLSTCVFDRGGNLYHGR
                     IKAVAEGAREGGLKF"
     gene            7656..8177
                     /gene="rpsE"
                     /locus_tag="PLY81_01350"
     CDS             7656..8177
                     /gene="rpsE"
                     /locus_tag="PLY81_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNF28906.1"
                     /translation="MSKVSDNKVKAGGDMELKEKVVAINRVVKTTKGGRTFSFSALVV
                     VGNENGVVGQGLGKAKEVQEAITKGIEDAKKNLVKVPIMRGTIPHEQFIKSGAAKVLI
                     KPAAQGAGVIAGGSMRAVLESAGFTDVQAKSLGSANPHNVVKATIKALTALREPVQVA
                     KNRNIKLSKVFNG"
     gene            8246..8428
                     /gene="rpmD"
                     /locus_tag="PLY81_01355"
     CDS             8246..8428
                     /gene="rpmD"
                     /locus_tag="PLY81_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNF28907.1"
                     /translation="MAKIKITQIKSGIDRTELQKQTLIALGLRKLNASKEVEATPQIL
                     GMVNKVSHLIKVEEIA"
     gene            8529..8978
                     /gene="rplO"
                     /locus_tag="PLY81_01360"
     CDS             8529..8978
                     /gene="rplO"
                     /locus_tag="PLY81_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNF28908.1"
                     /translation="MKLHSLKPAKGSTHKEKRLGRGEASGKGGTSTKGNKGGQSRAGY
                     QRKNAHEGGQMPIQRRVPKRGFKNINRVEYQVFNLGQIEQLQEKYALPEFSLENLYVN
                     GLISRTDKVKVLATGEIKSKLNFKINAISDKAKAAIEALGGTVEIVK"
     gene            9058..10413
                     /gene="secY"
                     /locus_tag="PLY81_01365"
     CDS             9058..10413
                     /gene="secY"
                     /locus_tag="PLY81_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNF28909.1"
                     /translation="MKKLIQTIKNIWSIEELRGKIIVTLLLILLYRFGTHIVLPGLDA
                     AALESIKASNKTEGLLGLFDAFAGGAFSQASILALGIMPYISASIFMQLMAILVPQLQ
                     KIQKEGESGRKKINQWTRYLTVIVTAFQASAYIAYLNSTYSAAIIPAYASFFPFSTVI
                     ILTAGSLFVMWLGEKIQDKGLGNGASIIIMVGILARLPISIIQEWTAKGEKGGGGLLI
                     FLIEIAVFIAVVVGLVLLVQGVRKIPVNYAKQIVGNRQFGGARQFLPVKVNSAGVMPI
                     IFAQAIMFLPTLVSFTNLDSKEGIVRIFNDHTHVVYMLVYAVMVIGFTFLYTALIFNP
                     KQIAEDLKRNNGFIPGVKPGQPTADYIGSIMDKITLPGAIFLAFVGILPGFADRLGVT
                     QSFSTFFGGTSLLIMVQVILDTLQQIETYLLMRQYDGLMSSGRVMGRQSSNSSLPTTT
                     I"
     gene            10482..11309
                     /gene="map"
                     /locus_tag="PLY81_01370"
     CDS             10482..11309
                     /gene="map"
                     /locus_tag="PLY81_01370"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNF28910.1"
                     /translation="MTLRPNGMIIYKTEAEVALMKQSALLVSKTLAEVAKVLKPGMNT
                     LEIDKMCEQFVRDHHAIPSFKNYKNTYPFSICASVNDVVVHGFPNTTPLKEGDVVSID
                     LGVILNGWHGDHAYTFILGETNNEVLQLVKVTKESLYKGIEKAIVNNRIGDIAFAIQE
                     HTEKKHGYGVVRELVGHGLGRSLHEDPQIPNYGKRGEGPKLKENLVIAIEPMINMGTK
                     DVFTDEDGWTVRTKDGKPSVHFEHDVCVKRNQALILSDYTFIEAAEKANTNLNTSYY"
     gene            11347..11772
                     /locus_tag="PLY81_01375"
     CDS             11347..11772
                     /locus_tag="PLY81_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28911.1"
                     /translation="MLNVEDDVVLDPYHYNEIATISNKFLTGMGCCSINDVAPLLTFI
                     LSTDYKKEFSKQEVIIKLKSFFEQRIGFQNFSHTIENIDFDINEGMDGYGLADGIVNY
                     NTTLDSAIINSENIHFKLFFIYEEKNWKIFFFKFPEFIW"
     gene            11891..>12190
                     /locus_tag="PLY81_01380"
     CDS             11891..>12190
                     /locus_tag="PLY81_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF28912.1"
                     /translation="MKRSITKVAVLGSGVMGSRIACHFAGIGVQVLLLDIQPKKEEGK
                     KHEPNKIVNDALATALKSNPSPIYTADVVKKITTGNFEDNMKDIATCNWIIEVVIE"
BASE COUNT         4753 a         1654 c         2278 g         3505 t
ORIGIN      
        1 aaatattaga aaccctttat ataaaggtgg gggtactatt tttggaccta aacctcatgg
       61 ctactcaatt aaattaaatc gtaaagtaaa agatttagca aaaatttctg cacttagcta
      121 taaggcaaaa gaaaatgcaa ttttggtggt agaagatatc aatttagatt ctcctaaaac
      181 aaaacaaatt gcagaagcta tgaatgcttt aaatgttgct gacaaaaaat caatattagt
      241 attaggagag tataatgata acgtttattt aagtactcgt aatataccta atgttgctag
      301 cacattatta gcagatatga atacatacga tattgtaaat gccgatgtaa tagtattaac
      361 agagaatgct gcaaaaattt ttagtgaaaa cgaagtagct acagaagctt aaaataatat
      421 aataatcgtt tcaatttaaa tacaatgaaa cttacagaag tattagtaaa accaatttta
      481 accgaaaagg caaatgcaca gcaagaaaaa ttgcgtagat atgcttttag agttaataaa
      541 aaagcaaata agcttgaaat taaaaaagct attgaacaat tttatggcgt aactgtaaca
      601 aatgttaata caacaattgc tgcaggaaaa aataaaacac gttataccaa agccggcttt
      661 attgaaggga aaaaagctgc ttacaaaaaa gcatttgtaa cagtagccga aggggaaaca
      721 atagatttat acgcaaacat ttaattatag ctaacgaagc tgaaaaattc gtttataaaa
      781 aagtaaaaac atggcattaa aaaagtttaa accagttaca gcaggcacac gctggaaaat
      841 tggtaacgct tatgcagaaa taacaactaa taaaccagaa agaagtttat tagaacctgt
      901 taaaaaaaca ggaggacgaa atgctcaagg tcgcagaaca atgagatata ttggtggtgg
      961 tcataaaaag cattatcgta ttatagattt taaacgtaat aagcaaaata ttgcagctac
     1021 tgttgtatca attgagtacg atccaaaccg cactgcattt atagcattat taaactatac
     1081 cgatggagag aaaagatata ttattgctcc acaaggttta caagttggtg caaccgtaat
     1141 tagcggtaat gaagcacctc cagaaattgg caatgcatta caaatgaaaa atatgccttt
     1201 aggtacaatg attcataata tagaaatgca accaggccaa ggtggtaaat tagtaagaag
     1261 tgcaggagcc tcagctcaac ttaccaacaa agaagaaaaa tatgcagttc ttaaaatgcc
     1321 aagtggtgaa ttaagaaaag tgttaatcaa ctgctttgca acggtaggtg ttgtttcaaa
     1381 tagcgatcat agcttagaaa ctgcaggtaa agctggtgca aatcgttgga agggaataag
     1441 accaagagta agaggtgttg caatgaaccc agttgatcac ccaatgggtg gtggcgaagg
     1501 tagagcatca ggtggtcacc ctcgtagcag aactggtaaa tatgcaaaag gagaaaaaac
     1561 aagaacaaaa ggaaaaggca gcgataaatt aattattcaa cgccgagatg gtaaaaaatt
     1621 aacaaaataa tttaaaagtt aaaatattct ctttaataag aggaaaataa acaaaataac
     1681 aatatggctc gttcaattaa aaaaggacct tatgttgatg ctaatcttga aggaaaagta
     1741 ttaggcatga atgacggaaa atcaaaaaaa ggtgtaataa aaacctggag tagaagaagc
     1801 acaattacgc cagattttgt tggccatacc tttgcagtac ataatggcaa taaatttatt
     1861 ccagtttatg ttacagagtt tatggtaggg cataaattag gagaatttgc accaactaga
     1921 cactttaaag gacattctgg aagtaaacaa taaaaaatta agaattgaga actatgaatc
     1981 aagaatattt aaactcataa ttaattcata actcataact cataactcat aactaaatca
     2041 aatggaagca gtagctaaat taaataatta ccctacaggg ccacgcaaga tgcgtttgtt
     2101 ggctgatgtt attagaggta tggaagttga aaaagccttg gctatattag agtttcatcc
     2161 gcaacataat gcaatgccat tagcaaagct attaaaaagt gctattaata attgggaaca
     2221 aaaaaataat ggcgaaagtg ctgctaatag tgggcttgtt gtaaaaactg tttttgtaga
     2281 tggtggtaga gttcttaaaa gaatgagacc agcaccacaa ggccgtgggt ataaaataag
     2341 aaaacgcagt aatcacgtaa caataattgt tgacactaaa taaaccaaaa acaaaattca
     2401 aaacttacac cacttaatta atgatgaagt tttgaaactt gtaatctaaa ataatactat
     2461 gggtcagaaa gcaaatccaa ttggcaatag gttaggtatc atccgcggat gggaaagtaa
     2521 ttggtatggc aataaaaaag attttgccgg aaaattaata gaggataata aaatccgtac
     2581 ttacctaaat gctcgtatta acaaaggtgg tatagctcgt attgttatag agcgtacact
     2641 aggtaaatta attgttacca tacatacaag taaaccaggt atcatcattg gtaaaggtgg
     2701 tggtgaggtt gatagaatta aagaagagct taaaaagctt actggcaaag aagatgtaca
     2761 aattaatatt ttagaaattc gccgccccga attagatgct caaatagtag gagatacaat
     2821 tgctcgtcaa atagaaaatc gtatcaactt taaaagagca actaaaatgg ctatagcctc
     2881 tacacttaga atgggtgcag aaggaataaa agtaaaactt agtggccgtt taggaggtgc
     2941 cgaaattgca cgtagcgaag agtttaaaca aggacgtact ccattacata cttttcgtat
     3001 ggatattgat tatgccaatg tatttgcaca aactgtttac ggtaaaattg gtataaaagt
     3061 atggatttgt aaaggagaag tattaggaaa aagagattta aatccaaact ttatcagtgg
     3121 caaaaatgaa aatgccggat ctgatagaag aggaggagat agagatagaa gaagtgcagg
     3181 tggagaaaga ggagacagaa gaggaggaag aggaagaaac taatttgata aaatgcacca
     3241 agcaagttta tcaagcatta acaaaaaaat attcaaatag caaaagatgt tacagcctaa
     3301 aagaagcaaa cacaggaaac aacaaaaagg tcgtattcgt gaagttgcca aacgtggtac
     3361 ttctatatca tttggatctt atgctctaaa agctttagag ccaatatggt taacaaacag
     3421 acaaattgaa gctgcccgtc aaagtatgac acgtgcaatg aaacgggaag gtaatgtttg
     3481 gattagagtt ttccctgata aaattattac acgtaaacca gccgaagtaa ggatgggtaa
     3541 aggtaaagga aacccagaat tttgggcagc agtggtagaa ccaggacgta tcatatttga
     3601 agtagatggc gtatctgaaa cagtagcaaa agaagcaatg ggtttagcat cacaaaaatt
     3661 accaataaaa acaaagtttg tagtaagaag agatttacaa gcttaaaata ataaaagtag
     3721 aatgttatgg caactaaaaa attcgacttc aataaaggca taaaagaaat taccgaagca
     3781 gatttaaaag ctcgtataac cgaagactct cttaggttaa aaaagctaga gtttgctcat
     3841 gctatttctc ctttagaaaa cccaatgaca atacgtgggt taagaagaga tattgcacgt
     3901 ttaaaaacag aattaaaact aagaacctta gcttaattga ttagctaaag ttaatattaa
     3961 ttttttagcc caaaaggcta tataaaagct aaaagcttaa aaaaatggtt gaaagaaatt
     4021 tgcgtaaaac aagagttggg gtagttacca gcaacaaaat gacaaaaact gtaactgttg
     4081 ctgttgaaag aaaagtaaaa caccctattt acggtaagtt cgtaaaaaaa actaccaaat
     4141 tccatgctca tgatgaaaaa aatgagtgca caattggcga tattgtaaaa ataatggaaa
     4201 cacgtccatt aagtaaaaca aaacgttgga gactggtaga agtagtagaa aaagcaaaat
     4261 aaataatttc cgatttgaga tttgagattt gtaattcaat tctgaattct aaatatcaaa
     4321 tctgaaattt caaatttcaa atttataaaa aatgattcag caagaaagta ggctaaatgt
     4381 agctgataac agcggagcaa aagaagtact ttgtattaaa gtactaggaa atagtggaca
     4441 ggattatgcc catattggcg atttaattgt agtaactgta aaagatgcaa tacctgcagg
     4501 cggtattaaa aaaggtaccg tttctaaagc agtaattgta cgtacaaaaa acaaattacg
     4561 tcgtaaagat ggatcttata tccgttttga tgataatgca gtagtgttat taaacacctc
     4621 taacgagcca agaggtactc gtatttttgg cccagttgcc agagaattac gggataaagg
     4681 ttatatgaaa attatttcat tagcaccaga ggttctataa ataattgccc aaagcaaata
     4741 gctgtaagca taaaaaaaat tagtaagtat tcgcttttag cataaagcta taagcattaa
     4801 gcatacaaaa tatgagtaca agatttacac ccaaatttaa catcaaaaaa ggcgatacag
     4861 tagtggttat tgctggcgat gataaagatt tgaaaaagcc acgtaaagtg ctaaaagtaa
     4921 ttgttgaaaa aagccgtgtg gttgtagaag gcgttaatat tattacaaag catactaaac
     4981 cttctgcaca aaacactaaa ggtggtatca ttaaaaaaga agcaccagtt catatttcta
     5041 atgtaatgtt gtgggatgct aaaaaaagtg ctcctactaa aataaaacgt tcaagagaaa
     5101 gtggcaaatt agtgcgtatt tctaaaaaaa gcggtgaagt aatttaattc atctggttta
     5161 aaaatataaa ttatgagtac tataaaatat aaacctcgtt tggcagataa gtacaagaat
     5221 gatgttgtac ctgctttggt taaaaaattt gcatacaaaa cagtaatgca aacacctaaa
     5281 ctagaaaaaa tttgcatcaa ccgtggagta aatggtgctg taaacgataa aaagttaatt
     5341 gacgtagcaa ttggcgagtt agaacaaatt actggtcaaa aagctgtgcc aacattaagt
     5401 aaaaaagata tttcgaactt taaactacgt aaaggaatgc caattggtgc aaaagttacc
     5461 ttacgtggag aaaaaatgta cgaattttta gatagattag tttctgtagc attacctcgt
     5521 gtacgtgatt ttaaaggcat tagtgataaa gctttcgatg gccgtggtaa ctacacttta
     5581 ggtataacag agcaaattat ttttcctgaa attgatattg ataaagtaaa taaaattaca
     5641 ggtttagata tcacttttgt tacatctgct caatctaacg aagaagcgta tgaactttta
     5701 aaagagttag gcttaccttt taaaaatgca aaaaaagata ataactaaat aaaaagtgct
     5761 gttacaagct acattttaaa cagaaattgt aaaacaatat ggcaaaaaaa tcaatcgttg
     5821 ctcgtcaaac aaaacgaaaa gctctagtag caaagtttgc agaaaaaaga gcagcattaa
     5881 aagaagcagg agattatgca gctttagata aactacctaa aaatgcatct ccggtacgat
     5941 taaaaaatcg ttgccaatta actggtcgtc ctaaaggtta tatgagatat tttggtatat
     6001 ctagagtaat gtttagagat atggctttaa atggtaaaat accaggtgtt aaaaaggcaa
     6061 gctggtaaca acaaaaaatc tctttgtaga gtaacaattt gaaacaatat ttaagaactg
     6121 atttaattta atacaaatgg taactgatcc tatagcagat tatttaacaa ggattcgtaa
     6181 cgctcaaatg gcgggtcata gaatagtaga aattcctgct tcaaatttga agaaaagaat
     6241 tactgaaata ttgtatgaac aaggatatat tttgagatac aagtttgaag atgatagcaa
     6301 acaaggtata attaaaattg ccttaaaata cgatgcttca accaagcaac ctgctattcg
     6361 taacttagaa agagttagtc gacctggttt acgtcaatat gcaaaaccta ctgaaattaa
     6421 aagagtaatt aatggcttag gtgttgcaat tattagtaca tcaaaaggtg tgttaacaga
     6481 taaacaagct aaagtacaaa acgtaggcgg agaagtactt tgctatatta gttaatataa
     6541 aattttgtaa agtaggtttg ataataacca ctttacatta acaatatgaa gacaatttaa
     6601 gcttactata cggtagctga acacttctaa aattatcaaa attgtagtat aaaaaaataa
     6661 aaataagatt atgtctcgta taggtaaaca acccatttca attccttctg gtgttactgt
     6721 tagcattgca aacggtaacg tagtaactgt aaaaggccca aaaggagaat tgaaagaatc
     6781 tattgaccgt gatattgcta ttgaggtaaa agatggtcaa atattaatca ctcgtccaac
     6841 cgatcaaatt cgccatagag cattacatgg cctatatcgt gccttaatca acaatttagt
     6901 taaaggcgta acagacggtt ataaaaaaga attagaatta gtaggggttg gttttaaagc
     6961 agctaacaca ggcaacactt tagatttagc tttaggatac tctcacaata ttatttttga
     7021 agtgcctaaa gaattaaaag ttgcaactgc taccgaaaaa ggtaaaaacc caattataac
     7081 tttagaagga tccgataaac aacttattgg tcaagtagct gctaaattaa gaagcctacg
     7141 taaaccagaa ccatataaag ggaaaggggt gaaatttgta ggtgaagtat taagaagaaa
     7201 agctggtaaa tcggctggta aataataaaa ttttataaaa atattaccgg cagaatcggt
     7261 aagtaaaaaa taaaatcatg agtagtaaca ttagtaataa gcaaaaaatt aagtatcgta
     7321 ttcgtaaaaa aattagcggt acggcagtaa agcctcgttt aagtgttttt agaagcaatg
     7381 cagaaattta tgctcaatta attgatgata acaatggcgt aacattaact gctgctgcaa
     7441 gtacagataa agaaatagct gcacaaaaaa gtactaaatc agaaaagtct aaactagtag
     7501 gtcaagctat tgcacgtaaa gcaatagaat taggtttatc tacatgtgtt tttgatcgtg
     7561 gtggtaattt atatcatggt cgtattaaag cagttgctga gggagcaaga gaaggaggcc
     7621 ttaaatttta ataaataaat ttcaaattaa caataatgtc taaagtaagc gataataaag
     7681 taaaagccgg cggcgacatg gaattaaaag agaaagtagt tgccattaat cgtgtagtaa
     7741 aaactacaaa aggtggtcgt acattttctt tctcagcact agtggttgta ggtaatgaaa
     7801 atggtgtagt tgggcaagga ttaggaaaag ccaaagaagt acaagaagct attacaaaag
     7861 ggattgaaga tgctaagaaa aatttagtaa aagttcccat catgcgtggt actatacctc
     7921 acgaacagtt tattaaatct ggtgcagcta aagttttaat taaacctgca gctcagggtg
     7981 ctggtgtaat tgctggtggt agcatgcgtg ctgttttaga aagtgctggt tttacagatg
     8041 ttcaagcaaa aagcttaggc tctgccaatc ctcataatgt ggtaaaagct actattaaag
     8101 cactaactgc attaagagag ccagttcagg ttgctaaaaa tagaaatatt aaattaagta
     8161 aagtatttaa cggataaatt aaattgaagg caaaaaatta aaactagtta cgttaatttt
     8221 tttaaacttc taaaacttat ttaaaatggc aaaaattaaa ataactcaaa ttaaaagcgg
     8281 tatagataga actgaattac aaaagcaaac tttaatagct ttaggtctta gaaaattaaa
     8341 tgcatctaaa gaggttgaag ctacacccca aatattagga atggtaaata aagtaagcca
     8401 tttaattaaa gttgaagaaa tagcttaaaa tattaaatgt ttgaactaat aactcaaaca
     8461 ttttattatt tcataaatta tcattgcgag gggtgatacc ccgtaagtcc aaaatcaata
     8521 aaaataacat gaaattacat tctttaaaac ctgcaaaagg tagtacacac aaagaaaaaa
     8581 gattaggccg tggtgaggca agcggtaaag gaggaacatc tacaaaagga aataaaggag
     8641 gccaaagccg tgcaggttac caacgtaaaa atgcacacga aggtggtcaa atgcctattc
     8701 aacgtcgtgt acctaaacgc ggatttaaaa atattaacag agtagagtac caagtgttta
     8761 acttaggtca aattgaacaa ttacaagaaa aatacgcttt accagagttt tcattagaaa
     8821 atttatatgt taatggttta ataagcagaa cagataaagt aaaagtatta gcaaccggtg
     8881 aaataaaatc taaactaaac tttaaaataa atgccattag cgataaagcg aaagcagcaa
     8941 ttgaagcttt aggcggtaca gttgaaattg ttaagtaatt ttcagctata ttgcacgaca
     9001 cattttaaaa tgtgtctttt tgattttatg tattaactgt ttcaatcgaa taatcaagtg
     9061 aaaaaactaa ttcaaactat aaaaaatata tggagcattg aggagctaag aggcaaaatc
     9121 attgttacat tattattaat tttattatac cgttttggta cacatattgt tttaccaggg
     9181 ttagatgcag cagccttaga gagcattaaa gcaagtaata aaaccgaagg tttgttaggt
     9241 ttgttcgatg catttgcagg tggtgccttt tcacaagctt ctattttagc tcttggcatc
     9301 atgccttaca tttctgcttc tatatttatg caattgatgg caatcttagt tcctcaatta
     9361 caaaaaattc aaaaagaagg agaaagtggc cgtaaaaaaa taaaccagtg gactcgttac
     9421 cttactgtaa ttgtaactgc ttttcaggca agtgcttata ttgcttattt aaatagcacc
     9481 tattcagcag caattattcc tgcttatgca agtttctttc cattctctac tgtaattata
     9541 cttacagcag gttcattgtt tgtaatgtgg cttggcgaaa aaattcaaga taaaggctta
     9601 ggcaatggtg cttctatcat tatcatggtg ggtatcttag ctagattgcc tataagcatt
     9661 attcaagagt ggactgcaaa aggtgagaaa ggcggtggcg gattattaat atttttaatt
     9721 gaaatagctg tatttattgc agtagttgta gggcttgttt tacttgttca aggagttaga
     9781 aaaattccgg taaactatgc aaaacaaatt gttggtaata gacagtttgg tggagcaaga
     9841 caatttttac ctgttaaagt aaatagtgct ggtgtaatgc ctatcatatt tgctcaggca
     9901 attatgtttt tacctacatt ggtttcattt acaaatttag attctaaaga aggtattgtt
     9961 agaatattta atgatcatac acatgttgtt tatatgttgg tgtatgctgt tatggtaatt
    10021 ggatttacct ttttatatac tgcattaatt tttaatccta aacaaattgc cgaagattta
    10081 aaaagaaaca atggctttat acctggtgta aaacctggtc agccaactgc agattatatt
    10141 ggctctatta tggataaaat tactttacca ggagctatat ttttagcatt tgttggtatt
    10201 ttacctggct ttgcagatag actaggtgta acccaatcat tcagtacatt ttttggaggt
    10261 actagtttat taattatggt acaggtaatt ttagatactt tacaacaaat agaaacttac
    10321 ttattgatgc gtcaatacga tggattaatg agtagtggta gagtgatggg gcgtcaaagt
    10381 tctaatagta gcttaccaac aacaactatt taatttttaa gttataaata tactagaaac
    10441 ctgttggagt aatatctaac aggtttctta ctttatatca aatgacatta agacccaacg
    10501 gtatgataat ttataaaaca gaagcagagg ttgctttaat gaagcaatca gcattattgg
    10561 ttagcaaaac acttgcagaa gtagctaaag ttttaaaacc cggaatgaat actttagaaa
    10621 tagataaaat gtgtgagcaa tttgtaagag accatcatgc cattccatct tttaaaaatt
    10681 ataaaaatac ttatcccttt tctatttgtg caagtgttaa tgatgttgta gttcacgggt
    10741 ttccaaatac tactccttta aaagaagggg atgtagtatc tattgacctt ggtgtaatat
    10801 taaatggatg gcatggagat catgcttata cttttatttt aggagaaact aacaacgaag
    10861 tgttacaact tgtaaaagtt accaaagaaa gcctctacaa aggaattgaa aaagctattg
    10921 taaacaacag aattggcgac attgcatttg caatacaaga acatacagaa aaaaaacatg
    10981 gctatggagt tgttagagaa ttggttggac atggtttagg aagaagttta cacgaagacc
    11041 ctcaaattcc taattatggg aaaagaggcg aagggccaaa gctgaaagaa aatttagtaa
    11101 ttgcaattga accaatgata aatatgggaa caaaagatgt gtttactgat gaagatggct
    11161 ggactgtaag aaccaaagat ggtaagcctt ctgtacactt tgaacatgat gtttgtgtaa
    11221 aaagaaacca agctttaata ctatctgact atacttttat tgaagctgct gaaaaagcaa
    11281 atactaactt aaatacaagt tattattaat ttcaattttg ctaccagcat aaaagcctaa
    11341 tataaaatgc taaatgtaga ggatgatgtt gttttagacc cttatcatta taatgaaata
    11401 gccactattt caaataaatt tttaactggt atgggttgct gtagtataaa tgatgttgcc
    11461 cctttgctaa catttattct ttctacagat tataaaaaag aattttcaaa acaagaagta
    11521 ataataaaat tgaaatcttt ttttgaacaa cgcataggct ttcaaaattt ttctcacaca
    11581 attgaaaata ttgattttga tattaatgaa ggaatggatg gctatggttt ggctgatggt
    11641 attgtaaact ataatacaac gctagatagt gcaataataa actcagagaa tattcacttt
    11701 aaactatttt tcatttacga ggaaaagaat tggaaaatat tcttttttaa atttccagaa
    11761 tttatttggt aaaattcagc atcttttatg tataaaaaga aaatagttgc atagctaact
    11821 attttttcct tagctttgtt ttcatacaaa tagaataatt tctttttgaa aaaatgtata
    11881 aaaaaatagt atgaaaagaa gtataacaaa agttgccgtt ttaggtagtg gggtaatggg
    11941 tagtagaatt gcttgtcatt ttgcaggtat tggtgtacag gttttgttat tagacataca
    12001 acctaaaaaa gaagaaggta aaaaacatga acctaataaa attgtaaatg atgcattagc
    12061 aactgcatta aaaagtaatc caagccctat ttatactgca gatgttgtaa aaaaaattac
    12121 tacaggaaac tttgaggata acatgaaaga tattgctact tgcaattgga ttattgaagt
    12181 ggtaatagaa
//
LOCUS       DAOQHP010000019        11925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2628_length_11925_cov_3.694356, whole genome shotgun sequence.
ACCESSION   DAOQHP010000019 DAOQHP010000000
VERSION     DAOQHP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2628_length_11925_cov_3.694356"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="bshC"
                     /locus_tag="PLY81_01385"
     CDS             complement(<1..689)
                     /gene="bshC"
                     /locus_tag="PLY81_01385"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HNF28913.1"
                     /translation="MKINCSHLTYKQTGFFSKIVLSYLNSDKELQPFFEHNTTNEGLK
                     NAIASRKNFKTNRKLLVEVLEQQYQNIPLTALQEKNLKALLHENTFTVTTAHQPNIFT
                     GPLYFIYKILHVIKLADDWNKHDNTNYFVPIYFMGSEDADFEELSFINLEGEKLSWKT
                     NQTGAIGRMLVDENLIQLIDKIAGQIGIYNFGNELITNIKLCYTLNKNIEQATIEFVN
                     ILFKEFGLLIL"
     gene            complement(745..1938)
                     /locus_tag="PLY81_01390"
     CDS             complement(745..1938)
                     /locus_tag="PLY81_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF28914.1"
                     /translation="MTVIGLIKEGKIPSDNRVALTPAQCKWLKINLPNVKILVESSAT
                     RCFSDAEYKQAGIEVTNDLTSCDILLGIKEVPVDMLIANKTYLFFSHTKKQQPHNRKL
                     IKAIIEKNITLIDYECLEHEDGQRIIGFGFFAGIVGAHNGIMAFGNRTGLFQLPRVYQ
                     CKDYRSLIHHYFGLKLPNIKIAVTGSGRVAHGIIEVMNLMGVHEVEADEYLEKKFSYP
                     VYVQLKGADLYTHQENKTYKREHFHQNPTEYRSRFLPYTKCTDILMNGVYWDENIPRL
                     FELTDVNERFVTTTIADITDDEHGSVPLNLGDQTIENPVYGVDKKTFLKTEPYLPSSI
                     DIVAVGNLPNELPRDASRYFGEQLLKYILGDILNGASDIITRATIINQGKLTKEFSYL
                     ENYVK"
     gene            complement(1965..4418)
                     /locus_tag="PLY81_01395"
     CDS             complement(1965..4418)
                     /locus_tag="PLY81_01395"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF28915.1"
                     /translation="MKSFLIGVVLFFSFHSNGSTISGFIKDSKGNILPYASILISGTT
                     KGTTANGKGYYSLQISPGSYTIVCQHIGFKSTEKHIEIRNTPITENFILEPQSYNLLD
                     VTVKTGGEDPAYEIIRNAIKNREKNLNEIRKFECEVYLKGQLQLGDYPKKFLGQKVDF
                     EDGDTSKKKMLFLSETIAKYSVEKPNDAKIEVVSTRVSGNSDGFGFSFPQIISFYENN
                     VQIGRGLNPRGFISPISHNALNYYKYKFMGTFFENGKEVSRIKVIPKRKYEPLFAGYI
                     SIIENEWRIFSTELYLLKEQQMQLLDTLHIQQMYVPIKDKWVIKQQVIYPAGKLLGFK
                     FFGSFVQVYSNFNINPTFKKKHFDNVLLKVNDSANKKAKHYWDSIRPVPLLEEEAIDY
                     VKKDSLEQVRKDPKYLDSLDKKNNKFSLLGLLLTGKTFQKRKTKESIAIEPLFFKINY
                     NTVEGWNLKLSPTYTKRYEGRRSITISPNLIYGFNNKHFNAFLSTRYIFGKKYVNNLS
                     FSAGKAVLQYNNNNPIEERDNTFSTLYYTKNYLKIYETWFTKINYRVGIGKGFDLTTN
                     FQYQDRLKLDNLTKIEQWKQFGNRIFTPNYPVELGAASVKSHKAAIASFGIQFRPGSK
                     YVELPGRTLNLGSKYPVISANLTFGIKGLLNSNVNFSKWNFDISDDINLKLGGVVKYK
                     FNVGGFLNAKTVYLQDYQHIQGNRTVTATQYLNSFQLAPYYAFSNTSSLMTSAHIAYH
                     LNGFLTNKIPVFKKWNCFFVLSGSALYIKPNLHYYETMFSIENIFKIIRIDLVQGFGD
                     NNYKTSGIRLSLPGFLTGN"
     gene            4566..5564
                     /locus_tag="PLY81_01400"
     CDS             4566..5564
                     /locus_tag="PLY81_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNF28916.1"
                     /translation="MKISNETKIGVLAVISIVLLIIGFNFLKGKSVFKTGNFIYANFT
                     NTKGILVSNPVLANGYRIGTVYEIVENDKSLQSIKVAIKLKKEYNIPSDSYAMIEANP
                     LGVTSIVIKLGSSKSLLTHNSTITTKENEGLITQLSNTITPTVDQVKNTFHTLDSTLK
                     NINSILDATTKNNLQVTVANINKLTASLLVSSANIEKMLVAQSGSIAQTMDNVNSFSK
                     NLADNNSKITNTIQNLETATGKLANSNIDNAVNSLQQAVDKMNTTLTKLNSSEGSLGL
                     LLNDKTLYNNLTNTVRSANILIDDLKTHPKRYVNISVFGKKDKSTPLKAPLSETKQ"
     gene            5568..7199
                     /locus_tag="PLY81_01405"
     CDS             5568..7199
                     /locus_tag="PLY81_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNF28917.1"
                     /translation="MFQKIIFIFIAFVAVCTAKAQKKIAPDRDTLQAGTLLNIIYAER
                     MNFKKEDSLNQFVSLAGNVQIKQGKTFFYADSAVLNQYLNILEAFGKVHINDADSIHT
                     YANYLKYIGKEKKAYLKGNVKLTDGKGILTTDELEYDVTTKIGTYLKGGKVVNGKTVL
                     TSKQANYYGETKDVYFINKVVLKDPDYTIITDTLLYNTYTNITTFVTATTILSGKTKI
                     KTRKGYYDLNKKKAYFGERPEIEDSTGTLKADEVAIDDSTGKSEFKGNVVYKGKDSTS
                     GFDIIANKVERDSKKGSIRATQAPILLIKQGKDSVLIRADTLFSAKLSDLLLERNVHD
                     LRDSSEGKLYKIPSTDKDTTSDKYFEAYSNVKIFSDSMQAIGDSLFYSLKDSIFRLYK
                     NPIAWSNVNQITGDTIYLYTQNKKPEKIRVFENALAISHAGSNYYNQVSGRTINGYFV
                     DGKLDSLRCKGSPASSVYYGVDDYGKFFGVNVATCDVIDLFFKNGEPNKVVFKNNLDG
                     ITYPMQQVNHDLIKVKGFKWLQHKRPKTKYEILSY"
     gene            7265..8455
                     /gene="nhaA"
                     /locus_tag="PLY81_01410"
     CDS             7265..8455
                     /gene="nhaA"
                     /locus_tag="PLY81_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNF28918.1"
                     /translation="MLQFLKNKFVNPLKEIIHDSRSIGIVLLACTILSLVLANISSIN
                     LTYTSFWNFSFNQSNEHSIPIGFLNLPNSILIIINDFLMAFFFFLAGMEIKRELTNGE
                     LSSYKKSILPIIAAIGGMLFPAIIYFAFNISTNHLHGWAIPTATDIAFTLGIASLLGK
                     RVPLSLKIFITALAIIDDLGAILVIAFFYGSHLHFTYLFISLLLTLLLILINKFKNKF
                     VILHIIVAFLLWYCMFNSGIHATLAGVIVAFIVPAKSISKLEMKLHTPVYFIVLPIFA
                     LANTAIIFPNNIPALLNTSLSWGIIAGLVFGKPLGIFIASFITIKTKLAQLPTKTNYM
                     QLAGAGILCGVGFTMSIFISSLAFNEPINQDTAKIAVLFASSLAIILGIVWFKIFAKP
                     YRKN"
     gene            complement(8459..9151)
                     /locus_tag="PLY81_01415"
     CDS             complement(8459..9151)
                     /locus_tag="PLY81_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNF28919.1"
                     /translation="MQLIKKNGIVLFWAVLFIDCFFVFSQQYQFHAITKVLLIPVLLF
                     YIILNSRKKHYNRSKTLIYTALICSWIGDILLLQNGDVFFIAGTTAFLTTHIFYSIFF
                     FRVHPINNARSYEVTIIATLVVGALIFAVANFLHNDLPKFFRIPFYVYAIAIGIMAIL
                     AANIYTNRGKQKLALQFFIPGAALFILSDVALATFKFKYTDENFLEVVVMITYGYAQC
                     FMAQGFAKYLKG"
     gene            complement(9200..10546)
                     /gene="tilS"
                     /locus_tag="PLY81_01420"
     CDS             complement(9200..10546)
                     /gene="tilS"
                     /locus_tag="PLY81_01420"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNF28920.1"
                     /translation="MKINLLENFLINWNNHFKEIANSNAHFFIAVSGGVDSVVLTDLM
                     SKTSFSFSILHCNFNLRNDESKRDEIFVQSLATKYNTTVLVEHFDTNSYALEHKLSIQ
                     VAARNLRYNWFKNIIEQNTTTNKKCFLLTAHQADDTIETVLMNIFRGTGLKGLHGISM
                     QNNYILRPLLFARRSEIEQYAKQNNLDWVEDSSNKKVDYTRNNFRLNIIPLIKEHFLT
                     VEENLLANIEKWKEAEVIYNLYINEQKQKLIEVKDNTIQIPILKLQQQKAVKTVLYEI
                     IANYGFTANQLKDAIELLDAATGKYIVSATHRILKNRKWVIITPLKSNNIQHFIIEQD
                     DKKIFFDSNSIEIKIIENSEIQSNSNIACLDLKKISFPLLLRKYKQGDYFYPLGMSKK
                     KKISRFFIDAKLSLTQKEDIWVMESNKKIIWLVGLRIDDRFKVTTQTKSILQLQVK"
     gene            10679..11143
                     /locus_tag="PLY81_01425"
     CDS             10679..11143
                     /locus_tag="PLY81_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNF28921.1"
                     /translation="MSIEVGKPAPSFSLVDTERKEVKLSDFKDSNVLLLFFPLAFTSV
                     CVKELCSVRDNIALYNNANAQVLGISVDSPFALAKFKAEQNLNFPLLSDFNKTASTSY
                     HSIYETFLGMNGVGKRSAFVIDKSGTIRYAEVLENAGDLPNFEAINKVLETL"
     gene            complement(11166..>11925)
                     /locus_tag="PLY81_01430"
     CDS             complement(11166..>11925)
                     /locus_tag="PLY81_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28922.1"
                     /translation="YITGLNKGDYEATVQLKKIISNKLEFLQLGFKNVNRNPSLIFNS
                     NHNYWVLPNLVTKKENTILLSAQYNNPKKYLSLSAKYYLVNNYTYFDSFFSAKQESTL
                     FYVLQISAEKKIKLSKNFNWYAEVHFQQSTANAPINVPMFLTRNRLAFEGNFFTNLFL
                     STGIELKYYTNYKSAGYSPFNGQYFYATNYTITNRPELDVFLHFRIKSFKAYLRLENV
                     QSLIPGNGKNNFALQNYANQKLWFRTGIWWNFVN"
BASE COUNT         4187 a         1813 c         1587 g         4338 t
ORIGIN      
        1 ataagtatca gtaaaccaaa ctctttaaat aaaatattta caaattcaat tgttgcctgt
       61 tctatatttt tatttaaggt gtagcatagt tttatattag taatcaattc attaccaaaa
      121 ttatatatgc caatttgccc agcaatttta tcaattaatt gaattaagtt ttcatccacc
      181 aacattctac caatagcacc tgtttgattg gttttccagc ttaacttctc tccttctaaa
      241 ttaataaaac tcaactcttc aaagtctgca tcttcgctac ccataaaata aataggcaca
      301 aaataattgg tattatcatg tttattccaa tcatctgcca gcttaataac atgcaaaatt
      361 ttataaataa aatataatgg cccagtaaaa atatttggtt gatgggcagt tgttacagta
      421 aaagtatttt cgtgtaataa ggcttttaaa tttttttctt gtaaggcagt aagaggtatg
      481 ttttggtatt gttgttctaa aacctctact aataattttc tattggtttt aaaatttttt
      541 ctagaggcaa tagcattttt taaaccttcg ttagtagtat tatgttcaaa aaaaggttgc
      601 aattccttat cactattcaa ataacttaaa actattttag aaaaaaagcc cgtttgttta
      661 taagttaaat ggctacaatt aatcttcata cctaaaattg aatactttga ttattcatta
      721 aaagtataac taaataatta aactttattt tacataattt tcaagataag aaaattcttt
      781 ggttagtttt ccctggttta ttattgttgc tcttgtaata atatcgctag ctccatttag
      841 aatatctcct aaaatatatt ttagaagttg ctctccaaaa tatctgcttg catctcttgg
      901 taattcattg ggcaaatttc caactgctac aatatctatg gatgaaggaa gataaggttc
      961 ggttttaaga aaagtttttt tatcaacgcc atatacagga ttttcaatag tttgatctcc
     1021 taaatttaat ggcactgagc cgtgttcatc gtcagtaata tcggcaattg ttgtagttac
     1081 aaagcgttca tttacatctg taagctcaaa tagtcttggt atattttcat cccaataaac
     1141 tccattcatt aaaatatcag tgcatttagt atatggcaaa aatcttgatc tgtattccgt
     1201 agggttttga tgaaaatgtt ctcttttata agttttattc tcttggtgtg tatataaatc
     1261 tgcacctttt aactgcacat acactgggta actaaacttt ttctctaaat attcatctgc
     1321 ctctacttca tgaacaccca ttaaattcat tacttctata ataccatgtg caactctacc
     1381 actacctgta acggcaattt ttatattagg aagttttaat ccaaaataat gatgtattaa
     1441 ggaacgataa tctttacatt ggtacactct tggcaactga aacaagcctg ttctattgcc
     1501 aaaagccata atgccattat gggcaccaac aatacctgca aaaaaaccaa aaccaataat
     1561 tctttgccca tcttcatgtt ctaaacattc ataatctatc agcgtaatat ttttttcaat
     1621 aatagctttt attagttttc tattatgggg ttgttgcttt ttggtatgac tgaaaaataa
     1681 gtaggttttg tttgcaataa gcatatcaac tggtacctct ttaatgccta aaagtatatc
     1741 acagctagtt aaatcgttgg ttacttcaat tccagcttgt ttatattcag catcactaaa
     1801 acacctggta gcactacttt ctaccaaaat ttttacatta ggtaaattta tttttaacca
     1861 tttacattgt gctggtgtta atgcaactct gttatctgat ggtatttttc cttccttaat
     1921 taatccaatt acagtcattc ttgtcttttg ggctgcaata taaattaatt accagttaaa
     1981 aaaccaggta aacttaacct aataccactt gttttataat tgttatcgcc aaaaccttgc
     2041 accaaatcta tcctaataat tttaaaaata ttttcaatag aaaacatggt ttcataataa
     2101 tgtaaattgg gttttatata caacgcactg ccagataata caaaaaagca attccatttt
     2161 ttaaataccg gaattttgtt agttaaaaat ccgtttaaat gataagctat atgtgctgaa
     2221 gtcattaacg aagaagtgtt gctgaatgcg taataaggag ctaactgaaa gctgtttaaa
     2281 tattgtgtag cagttacagt tctgttacct tgtatatgct gataatcttg taaataaaca
     2341 gttttggcat ttaagaaccc acctacatta aatttatatt ttactacgcc acctaatttt
     2401 aaatttatat catcgctaat atcaaaattc catttactaa aattaacgtt gctatttaat
     2461 aagcctttaa ttccgaaagt taagtttgca ctaatcacag ggtatttaga acctaagtta
     2521 agtgttctac ctggcagttc tacatattta gatcctggtc taaactgtat tccaaaactt
     2581 gctattgcag ctttatgact ttttacactt gctgcaccta attctacagg atagttaggg
     2641 gtaaaaattc tatttccaaa ctgtttccat tgctcaattt tagttaaatt atctaatttc
     2701 aatctatctt gatattgaaa gttggtagtt aaatcaaatc ctttaccaat accaactcta
     2761 tagtttattt tagtaaacca ggtttcataa atttttaaat aatttttggt gtaatacaag
     2821 gtagaaaaag tattatctct ttcctcaata gggttgttat tattatattg taagactgct
     2881 tttcctgcag aaaaagacaa attgtttaca tatttttttc caaaaatata ccttgtactt
     2941 aaaaaggcat taaagtgttt attattgaaa ccgtatatta aatttggtga aatagtaatt
     3001 gatcttcttc cttcatatct ttttgtataa gttggagata acttcaaatt ccacccttct
     3061 actgtattat aattaatctt aaaaaataaa ggctcaatgg ctatactttc ctttgttttt
     3121 cttttttgaa aagtttttcc tgttaataaa aggcctaata gagaaaattt gttatttttt
     3181 ttatctaatg aatctaaata tttagggtct tttcttactt gctctaaact atctttcttc
     3241 acatagtcta ttgcttcttc ctctaacaat ggtactggcc taatactatc ccaatagtgt
     3301 ttagcttttt tgttggcact atcatttact tttaaaagta cattatcaaa atgtttcttt
     3361 ttaaaagtag gattaatatt aaaattggaa taaacttgaa caaagctgcc gaaaaattta
     3421 aaacctaata atttaccagc aggataaatt acttgctgct taataaccca tttatctttt
     3481 ataggaacat acatttgttg tatatgtaat gtatctaaca actgcatttg ctgctctttt
     3541 agtaaatata actctgtact aaaaattctc cattcatttt caataatact aatataacct
     3601 gcaaataatg gttcgtattt tcttttagga attactttaa ttctacttac ttcttttcca
     3661 ttctcaaaaa aagtacccat aaatttatat ttataataat taagggcatt atggctaatg
     3721 ggcgaaataa accctcttgg gtttagccct ctacctattt gtacattgtt ttcgtagaaa
     3781 gaaataattt gaggaaaact aaaaccgaaa ccatcactat taccactaac tctggtagag
     3841 actacttcta ttttagcatc atttggtttt tctacagaat atttggcaat tgtttcgctt
     3901 aaaaaaagca tctttttttt gctggtatct ccatcttcaa aatctacttt ctgccccaaa
     3961 aattttttag gataatcgcc caattgtagt tggcctttta agtaaacttc acattcaaac
     4021 tttcttattt cgtttaaatt tttttctcta ttttttatag catttctaat tatttcatag
     4081 gcaggatctt cgcctcctgt ttttactgta acatctaaca ggttataaga ttgtggctct
     4141 aatataaaat tttcagttat gggtgtattt cgtatctcaa tatgtttttc agtactctta
     4201 aaccctatat gttggcaaac aattgtataa ctaccaggtg atatctgcaa tgaataatat
     4261 cctttaccat tggcagtagt gcctttggtt gttccactaa taagtattga tgcataaggt
     4321 agtatattcc ctttagaatc tttaataaaa ccacttattg tagagccatt agaatgaaaa
     4381 gaaaaaaaca atacaacccc tatcaaaaaa gacttcataa ataattttgt gtaaaaacga
     4441 aaataaataa caaaaagtta caaaaaattg aaaatgttaa ttttagcatt tattaaaata
     4501 taatttttta atctttaaca aggttaacca tttgaccact actttttaac ccttagattt
     4561 gctacatgaa aatttctaac gaaaccaaga ttggagtatt agctgttatt tctattgtgt
     4621 tattaattat aggatttaat tttttaaaag gaaaaagtgt ttttaaaact ggtaatttta
     4681 tttatgccaa ctttaccaac actaaaggca ttttggtttc taaccctgtt ttagctaatg
     4741 gatatagaat aggaaccgtt tatgaaatag tagaaaacga taaatcctta caaagcatta
     4801 aggtagccat taaattaaaa aaagaataca atataccatc cgattcttat gcaatgattg
     4861 aagcaaaccc tttaggagta acaagtattg taataaaatt aggcagtagt aaaagtttat
     4921 taacacataa ttcaaccatt actactaaag aaaatgaagg cttaattact caactttcca
     4981 ataccattac gcctactgta gatcaagtga aaaatacttt tcacacttta gattcaactt
     5041 taaaaaatat caattctatt ttggacgcta caactaaaaa taatttacaa gtaacagtag
     5101 ccaatataaa taagcttaca gcatctttac ttgtaagctc tgctaatatc gaaaaaatgt
     5161 tggttgcaca aagtggtagc attgcacaaa ctatggataa tgtaaacagt ttttctaaaa
     5221 atttggcaga taacaactca aaaattacca acaccataca aaatttagaa actgcaactg
     5281 gaaagttagc caactcaaat attgataacg ctgtaaacag tttacaacaa gcagtagata
     5341 aaatgaacac aaccttaacc aaattaaata gcagtgaggg tagtttagga ctgttactta
     5401 acgataaaac cttgtacaat aatttaacta atactgtacg tagtgctaat attttaattg
     5461 atgatttaaa aacacatcct aaacgctatg taaatatttc ggtatttggt aaaaaagata
     5521 aaagtacacc attaaaagct cctctttctg aaaccaagca ataataaatg ttccaaaaaa
     5581 taattttcat atttattgca tttgttgctg tttgtactgc caaagctcaa aaaaaaattg
     5641 cacccgatag agatacatta caagctggta ctctattgaa tattatttat gcagaaagaa
     5701 tgaattttaa aaaagaggat tcactcaatc agtttgtttc tttagcaggt aatgtacaaa
     5761 ttaaacaagg taaaaccttt ttttatgcag atagtgcagt tttaaatcag tacttaaata
     5821 ttctagaagc ttttggcaag gtgcacatta atgatgcaga cagtattcat acttacgcaa
     5881 attatttaaa gtatattggt aaagaaaaaa aagcttattt aaaaggcaat gtaaaactta
     5941 ccgatggcaa aggcatacta actactgatg agttagaata tgatgtaact acaaaaattg
     6001 gtacttactt aaaaggtgga aaagttgtaa atggtaaaac agttttaacc agcaagcaag
     6061 caaattatta tggagaaacc aaagatgttt attttattaa taaagtagtt ttaaaagacc
     6121 cagattatac cattattaca gatactttat tgtataatac ttatacaaat attaccactt
     6181 ttgtaacagc aaccactatc ctttctggaa aaacaaaaat taaaacaaga aaaggttatt
     6241 acgatttaaa caagaaaaaa gcctattttg gcgaaagacc tgaaattgaa gatagtacag
     6301 gaaccttaaa agcagatgaa gttgccattg atgacagcac aggaaaatct gaatttaaag
     6361 gcaatgtagt ttataaagga aaagattcaa ccagtggctt tgatattatt gcaaataaag
     6421 tagaaagaga tagtaaaaaa ggaagtatta gagcaacaca agctcctatt ttattaatta
     6481 aacaaggaaa ggatagcgtt ttaattagag cagatacttt attttctgca aaattatctg
     6541 acttattatt agaaagaaat gtacatgatt taagagactc ttccgaagga aaattatata
     6601 aaatacccag tacagacaaa gacacaacaa gcgataaata ttttgaagct tacagcaatg
     6661 tgaaaatatt ttcagactct atgcaagcca ttggtgatag tttattttac agtttaaaag
     6721 actctatttt caggctatac aaaaacccaa ttgcatggtc taatgtaaat caaataacag
     6781 gcgatacaat ttatttatat acacaaaata aaaaaccaga aaaaattcgt gttttcgaaa
     6841 acgcattagc aataagtcat gcaggaagta attactacaa ccaggtttcg ggtagaacaa
     6901 ttaatggcta ctttgtggac ggcaaattag attccttaag atgcaaaggc agccctgcct
     6961 ctagcgttta ttatggagtg gatgattatg gaaaattttt tggtgttaac gttgccactt
     7021 gcgatgtaat tgatttattt tttaaaaacg gagagcccaa taaagtagtt tttaaaaata
     7081 atttagatgg tataacttat cctatgcaac aagtaaatca tgatttgata aaagtaaaag
     7141 gctttaaatg gttgcaacac aaacgcccta aaactaaata tgaaattttg agttattaag
     7201 gattgtagga aaaatttctt cttaaatcgt tttacattct aaattctttc ttttacttta
     7261 gattatgtta caatttctta aaaataaatt tgtaaaccct ttaaaagaaa taatacacga
     7321 tagccgttcc attggtattg tgttattagc ttgtaccata ctttcattag ttctagccaa
     7381 tatctcttct atcaatttaa cctatactag tttctggaat tttagtttca accaaagtaa
     7441 tgagcatagc ataccaattg ggtttttaaa cttaccaaat tcaatcctca ttattatcaa
     7501 cgattttttg atggcattct ttttcttttt agctggtatg gaaataaaaa gagaattaac
     7561 caatggagaa ttgtcttcct ataaaaaatc tatcttacct ataattgcag ctataggcgg
     7621 tatgttgttt cctgcaatta tttattttgc ttttaatatc tcaaccaacc atttgcatgg
     7681 ttgggcaata ccaacagcaa ctgatattgc atttaccttg ggtattgcat ctttattagg
     7741 taaaagagtt cccctttctt tgaaaatttt tattacagca ttagcaatta ttgacgattt
     7801 aggtgccatt ttagtaattg cctttttcta tggatcacat ttacatttta cttatttatt
     7861 tataagttta ctactaacct tattactaat actaataaat aaatttaaaa acaagtttgt
     7921 aatactgcat attatagttg cttttttact ttggtattgt atgtttaact ctggcataca
     7981 tgctacactt gcaggtgtaa ttgttgcttt tattgtacca gccaaaagca taagtaaatt
     8041 agaaatgaaa ttacataccc ctgtttactt tatagtttta cccatctttg ccttggccaa
     8101 tacagcaatt atttttccaa ataatattcc tgctttatta aacacatctt taagttgggg
     8161 tattattgca ggtttagttt ttggtaagcc cttaggtatt tttattgcaa gttttataac
     8221 aataaaaact aaattagctc aactacctac caaaactaac tatatgcaat tagcaggtgc
     8281 aggtatttta tgtggagttg gttttaccat gagcatcttt atttcttcac ttgcatttaa
     8341 cgaaccaatt aatcaggata cagcaaaaat tgcagttttg tttgcctcta gccttgctat
     8401 cattttagga attgtttggt ttaagatttt tgccaaacca tatcgaaaaa actaagtact
     8461 atccctttaa atatttggca aacccttgtg ccataaagca ttgggcataa ccataggtta
     8521 tcattactac aacttctaaa aagttttcgt cggtgtattt aaatttaaaa gttgccaaag
     8581 caacatcaga taaaataaat aatgcagccc caggaataaa aaattgtaat gccaatttct
     8641 gcttacccct attagtataa atattggctg ctaaaattgc cataatgcca attgcaatag
     8701 catacacata aaatggtatt ctaaaaaact tgggtaaatc gttgtgtaaa aaatttgcaa
     8761 cagcaaatat taatgcacct acaactaatg ttgcaattat tgttacttcg taacttcttg
     8821 cattgttaat agggtgaacc ctaaaaaaga aaatagaata aaaaatatgg gttgtcaaaa
     8881 atgctgttgt tcctgcaata aaaaaaacat ctccattttg tagtagcaaa atatctccaa
     8941 tccaagagca aattaaagct gtataaataa gggttttaga acgattataa tgtttttttc
     9001 tagaatttaa aattatataa aacaataaaa caggtatcaa taaaaccttt gtgattgcat
     9061 ggaattgata ttgttgagaa aaaacaaaaa aacaatcaat aaaaagaaca gcccaaaaaa
     9121 gcacaatacc attttttttt attaattgca tttagtttta ttttaatttt aaatataata
     9181 atgctgcaaa aataactaac tatttaactt gaagttgtaa aatgcttttg gtttgggttg
     9241 ttactttaaa tctatcatca attcttaacc caacaagcca aataattttc ttattgcttt
     9301 ccattaccca aatatcttcc ttttgcgtta aagaaagttt agcatctata aaaaaacgag
     9361 aaattttttt ctttttagac attcctaaag ggtaaaaata atctccttgt ttatattttc
     9421 tcagcagtaa aggaaaactt atctttttta aatctaaaca tgcaatgttt gaattagatt
     9481 gtatttctga attttctata atcttaattt caatggaatt agaatcaaaa aatatttttt
     9541 tatcatcttg ttcaattata aaatgctgaa tattgttaga ttttagtggc gtaataatta
     9601 cccattttct atttttaagt atcctatgcg ttgcagaaac aatatattta ccagtagcag
     9661 catctaataa ttcaatagca tctttcaatt gattagctgt aaaaccatag ttagcaataa
     9721 tttcatataa aactgtttta actgcttttt gttgttgtag ttttaatata ggaatttgaa
     9781 ttgtattatc tttaacttct atcagtttct gcttttgttc atttatataa aggttgtaaa
     9841 taacttctgc ttctttccat ttttcaatat tggctaataa attctcttca acagttaaaa
     9901 aatgttcctt aattaatggt ataatattta acctaaaatt atttcttgta taatccactt
     9961 ttttattaga agaatcttca acccaatcta aattattttg tttggcatat tgttcaattt
    10021 cactccttct agcaaataat agtggcctta aaatgtaatt gttctgcata ctaattccat
    10081 gaagtccttt taaaccagta cctctaaaaa tattcatcaa aactgtttca atagtatcat
    10141 ctgcctgatg tgctgtaagt aaaaaacatt ttttattggt tgtagtattt tgttcaatta
    10201 tatttttaaa ccaattgtac cttaagtttc ttgcagcaac ttgtatagat aatttgtgtt
    10261 ctaaagcata agaattagta tcaaaatgtt ctactaaaac agtggtattg tattttgtag
    10321 ctaatgattg tacaaatatt tcatctcttt tactttcatc atttctcaaa ttaaaattgc
    10381 agtgtaaaat agaaaaacta aaagaagttt tagacattaa atctgttaat actacactat
    10441 ctacgccccc acttactgca ataaaaaaat gagcgttact atttgcaatt tctttaaaat
    10501 ggttattcca attaattaaa aaattctcga gcaagtttat tttcacttgt ataatttact
    10561 attttggcaa aaatatttta tattagtcaa ataaccatca aaacaaatgt tttacagcat
    10621 gcctaagctt gtttattaca tatttgaatt gtagatttac atttcaaaaa aaaatattat
    10681 gagtattgaa gtaggcaaac cagcaccttc tttttcttta gtagataccg aaagaaaaga
    10741 agtaaaactt tctgatttta aagattctaa tgtattacta ttattttttc ctttagcttt
    10801 tacaagtgta tgcgtaaaag agctatgtag tgtaagagat aatattgcat tgtacaataa
    10861 tgcaaatgca caagtgcttg gcatttcggt tgattctcca tttgctttag caaaatttaa
    10921 agcagagcaa aatttaaatt tccctttatt aagcgatttt aataaaacag catctacaag
    10981 ttatcattcc atatatgaaa cttttttagg catgaatggt gttggtaaac gttctgcttt
    11041 tgtaatagat aaatcgggta ctatacgtta tgcagaagta ttagaaaatg ctggtgattt
    11101 accaaatttt gaagcaatta ataaagtttt agaaacactt taactttact taaaccgatc
    11161 tgaatttaat ttacaaaatt ccaccaaatg cctgttctaa accatagctt ttggttggca
    11221 taattttgta aagcaaaatt atttttacca ttacctggaa ttaagctttg aacgttttct
    11281 aatcttaaat aggctttaaa acttttaatt ctgaaatgta aaaacacatc caattcaggt
    11341 cggtttgtta ttgtataatt ggttgcataa aaatactgac cattaaaagg agaataacca
    11401 gcacttttat aattggtata atactttaac tctatccctg tagataaaaa taggttagta
    11461 aaaaaattac cctcaaaagc caatctattt cttgttaaaa acataggtac atttattggt
    11521 gcattggctg tagattgttg aaaatgaacc tcggcatacc aattgaaatt tttagataat
    11581 ttaattttct tctctgcact aatttgcaga acataaaaca aagtactttc ttgtttggcc
    11641 gaaaaaaaac tatcaaaata agtatagtta ttgactaaat aatactttgc agataacgat
    11701 aaatattttt ttggattatt atattgagca gatagtaaaa ttgtgttttc ttttttggta
    11761 accaggttag gcagtaccca ataattatgg ttactattaa aaattaaaga tggatttcta
    11821 tttacatttt taaaccctaa ttgtaaaaat tcaagcttgt tgctaattat tttttttaat
    11881 tgaactgttg cttcataatc tcctttgttt aaaccagtaa tatat
//
LOCUS       DAOQHP010000020        11905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2638_length_11905_cov_3.827595, whole genome shotgun sequence.
ACCESSION   DAOQHP010000020 DAOQHP010000000
VERSION     DAOQHP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_11905_cov_3.827595"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLY81_01435"
     CDS             <1..221
                     /locus_tag="PLY81_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNF28923.1"
                     /translation="VSILTKVTITNVATKKTVTYQIVGEKEADLKMGKISSSSPIGKG
                     LIGKQIGEIAEVQAPNGILKFKVEEIAI"
     gene            complement(275..1111)
                     /locus_tag="PLY81_01440"
     CDS             complement(275..1111)
                     /locus_tag="PLY81_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HNF28924.1"
                     /translation="MATLIADCGATKCDWSLLENNKVKKIVTQGFSLYFLSAENVKQL
                     LITELLSKIKNTTVLKVYFYGTGIGNKVNAQQMKQILKQIFKGALIEVNTDLLAAARS
                     LCGNQKGIACILGTGSNSCYYDGKKIIKSNPSLGFVLGDEGSGAYLGKKVLQYYLYQT
                     FDEELTINFDKSYNTNKATILNSIYKSNTPNRYLASFASFLSENRGHYMIENIIEDGL
                     NDFFQNHIYKYRESWLHPIHFTGGIAYTYKDVIKELCHSYELEVGNFSKSPMQGLLQY
                     HQ"
     gene            1299..2888
                     /locus_tag="PLY81_01445"
     CDS             1299..2888
                     /locus_tag="PLY81_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF28925.1"
                     /translation="MDNNKKQIWLPLFFAIVMIFGMLVGYNLHKHTTSSNFFSSGKTN
                     SIQEVVSLIQNKYVDKIKIDSISEATINDLLSHLDPHSIYIPQNDVQAINDDLIGNFY
                     GIGVEFQMFEDTVTVISVVKNGPSEKAGVQAGDKIIFVNDTTKIAGVKITTDVIRNLL
                     RGEENSKVNITVIRDQKQQKITITRGSIPVSTIDASYMIEPQTGFIRLNKFGEKTYEE
                     FMEQLEKLQKEGMKNLILDLRGNTGGLLNEAVDIADEFLSNNKLVVYTEGAKSARYEF
                     KCKRDGLFEEGKLIILVDETSASASEVLAGALQDWDRATIIGRRTFGKGLVQQQFSLS
                     NGGALRLTVSRYYTPLGRNIQKSYTNKSKEEYRRDALGIEPHEKYENEDSAHPKPSFK
                     TPSGKTVFGGGGILPDILVTTDSITLTNNIAKIYHTNFLNKVALKLYIQNKNYLSKIN
                     SADKFYAEKLPTENDLSLVEKIAKSDSISLQKFSEKEKETLLFSIKKLIAKQMFGNNG
                     YYQLANKTDKVVLKAMEQVKQ"
     gene            2940..3176
                     /locus_tag="PLY81_01450"
     CDS             2940..3176
                     /locus_tag="PLY81_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HNF28926.1"
                     /translation="MEENEKIYILKKKKYIYGPYNFETLKQNNLKAGDMIWYEGLKDW
                     VPVEEIEGLASFIISDNTNNNGSKKSFFKKILGL"
     gene            3331..3696
                     /locus_tag="PLY81_01455"
     CDS             3331..3696
                     /locus_tag="PLY81_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28927.1"
                     /translation="MKRSKQYLLFILLFGAFINLGSYNSVFSQIFDHNGNTLNGTGST
                     STNSGIDEPVFEDNSTSTGNTAANGGGFAGTTSVDADDDDPGGPIDPIDDIPLDGGVA
                     VLLAVGIASGYNASRKKII"
     gene            complement(3765..5057)
                     /locus_tag="PLY81_01460"
     CDS             complement(3765..5057)
                     /locus_tag="PLY81_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF28928.1"
                     /translation="MKYLLIILGLLFCNIKSFAQTETEQVNNAVLQIKNKVIEWRRHF
                     HQFPELSNREFNTSKKVAEHLKNLGLEVRTGIAKTGVVGILKGGKPGPVVALRADMDA
                     LPVTERNNLPYKSTVTTTFNGQNTGVMHACGHDAHTAMLMGAAEVLSKLKASVPGTVV
                     FIFQPAEEGAPEGEEGGASLMVKEGVMDNPKVDVVFGMHIESDIPAGDLEYKEGAFMA
                     SADLFKIKVKGKSSHGSQPWLGVDPIYVSAQIINAFQGIVSRNSDLTKAPVVITVAAI
                     HSGLRFNIIPEEAEMLGTVRNLDKDIRIKVLEELKAIAEGIAKLHHATAEVTIENKTL
                     VTYNTPQLVKKMLPSLEKAAGKNNVRSRTWVTGAEDFSFYGEKAPAFFFYIGGMSATI
                     KSEQAAPHHTPDFIIDDSKLDVGVKALCQLVFDYSKVK"
     gene            5272..5796
                     /gene="pyrR"
                     /locus_tag="PLY81_01465"
     CDS             5272..5796
                     /gene="pyrR"
                     /locus_tag="PLY81_01465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNF28929.1"
                     /translation="MKTILSEPEIAITLKRLAYQIIEGDVQLRNTVFIGIQPRGIFFS
                     NRIVAEIKNIIEPINVEYGKLDITFYRDDVRNELHIANKTDINFSIENKRVVLIDDVL
                     WTGRTIRAALDALIDFGRPAKVELCVLINRRFNRELPIQPDYTGKAIDTFISEKVKVA
                     WKENDEKEEVILIQ"
     gene            5845..6771
                     /locus_tag="PLY81_01470"
     CDS             5845..6771
                     /locus_tag="PLY81_01470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNF28930.1"
                     /translation="MKLSTKHLLGIKDLHKEDIQLILSTANNFKDVLQRPVKKVPSLR
                     DITIVNLFYENSTRTRISFELAEKRLSADIINFTASGSSAAKGETLLDTVNNILSMKV
                     DMVVMRHSASGAPHFLAKHIPAAIVNAGDGINEHPTQALLDAFSMQEKFGSLEGLKVA
                     IVGDIMHSRVALSNIYLLQKMGAEVVVTGPPTLIPKYITEALGVKVEYNIKNALQWCN
                     VANVLRIQLERQNQPLFSSLREYNLSYGISKKLIDSLNKEITIMHPGPINRGVELDSD
                     IADGSSSIILNQVENGVAVRMAVLYLLANREN"
     gene            6806..7279
                     /gene="coaD"
                     /locus_tag="PLY81_01475"
     CDS             6806..7279
                     /gene="coaD"
                     /locus_tag="PLY81_01475"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNF28931.1"
                     /translation="MSSRICLFPGTFDPVTLGHVDIINRALPLFDKIYIGIGENAAKK
                     PMFTAEQRLVWFKDIFNNEPKIESVIFQGLTIEYCKKINARFILRGIRYVSDFEYEKT
                     IADANRTLDRTIETIFLTGEPKYTSVASTIVRDILKNGGDASPFLPEAVINSLKK"
     gene            7301..7735
                     /locus_tag="PLY81_01480"
     CDS             7301..7735
                     /locus_tag="PLY81_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNF28932.1"
                     /translation="MIWYNKQIAIKDFAHWGKNTMGEFLDIQFIEIGADFLKATMPVN
                     TKTHQPYGLLHGGASATLAETVGSVASALVINTEKQLCVGLEINANHIRSVKSGIVTA
                     TAKPLHLGATTHVWDIKITDENEKLICVSRLTVAILNKKITH"
     gene            complement(7725..8822)
                     /gene="ychF"
                     /locus_tag="PLY81_01485"
     CDS             complement(7725..8822)
                     /gene="ychF"
                     /locus_tag="PLY81_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNF28933.1"
                     /translation="MSLKAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIEPNTGL
                     VDVPDYRLSKLAELVKPNRIVPTQLEIVDIAGLVRGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDENVLREEGAINPVGDKEIIDIELQLKDLESVEKKIQRVEKLSRTDVKMKA
                     EYDVLMQCKLHLEKGNSIRSLGLSKEERVAISDLFLLTEKQVLYVANVDEASMHTGNK
                     YSEALKINAKAEGADVIVMCNNIEAQIAEMEAEEDKQMFMEEYGMKEPALDRLIQTTY
                     KILNLYTYFTAGVQEVRAWTIHQGWKAPQAASVIHTDFEKGFIKAEVIAYDDFVKYGN
                     EAAARENGRLRIEGKEYIVQDGDVMHFRFNV"
     gene            complement(8958..9389)
                     /locus_tag="PLY81_01490"
     CDS             complement(8958..9389)
                     /locus_tag="PLY81_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF28934.1"
                     /translation="MKEKKKIIAAGGLVSNNNGDLLLIYRKKKWDLPKGKFEEGESIE
                     QCAIREVQEETGLQNVAITNFVGKTYHEYYEKWISQHVIKETWWYAMQITTNEPFLPQ
                     VEEDIEEVIWADENKINQCLTNSFSNIKSIIIQWKEQKNKF"
     gene            9513..10145
                     /gene="pyrE"
                     /locus_tag="PLY81_01495"
     CDS             9513..10145
                     /gene="pyrE"
                     /locus_tag="PLY81_01495"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNF28935.1"
                     /translation="MTNEKAVAEKLLQVQAIQLSPNAPFTWASGWKSPIYCDNRKVLS
                     FPFIRDFVKSEMCNIIFEKFEDIELLAGVATAGIAWGAMAADQLKLPYIYVRPKPKEH
                     GLGNQIEGFYEKGQKVLVIEDLISTGKSSMQVVNVLRNQGLEVVGIVSIFTYQFSEAV
                     QLFEENNLQHISLTNYTNLIDLAIEKQLVNAELQNVLLDWRDNPATWNGI"
     gene            complement(10157..10852)
                     /locus_tag="PLY81_01500"
     CDS             complement(10157..10852)
                     /locus_tag="PLY81_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF28936.1"
                     /translation="MAETVVQLRGVNIYQGNSLILQDVNFTIESGEFVYLVGKTGTGK
                     SSLLKTLYGELPLKEGSCNVVGFDLKDMNWKKVPFLRRNLGVVFQDFQLMTDRNVHEN
                     LKFVLKATGWKDEKLIEEKINDVLNKVGLRSKGFKMPYEMSGGEQQRVDIARALLNSP
                     KLILADEPTGNLDPETSDEIMQLLFQITKDYGTAIIMATHDFIVINRYPSRMLTTDRS
                     RVIDTTVAATMHS"
     gene            complement(10945..11904)
                     /gene="murB"
                     /locus_tag="PLY81_01505"
     CDS             complement(10945..11904)
                     /gene="murB"
                     /locus_tag="PLY81_01505"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNF28937.1"
                     /translation="MNVIAKLFAAFNSVQQLIELLENNTDTTLIIGGGSNILFTKNFD
                     GLVLKNELKGIELICEDDEHYYIKVAAGEVWHQFVLYCIHNNYNGIENLSLIPGNVGA
                     SPMQNIGAYGVEIKDTFYELEALHIQEKTIVKFNNSDCAFGYRESIFKGKFKNQFIIL
                     NVTFKLHKKNIFNHSYGAIQQELDKMKVTALSAKAISDAVISIRSTKLPNPAIIGNAG
                     SFFKNPVIAKTQYHKLLQQFPKLPGYNDKDDTIKIPAAWLIEFCGWKGFRKNDVGCHT
                     NQALVLVNYGNATGLEIYNLSQDIINDVYNKFSILLQREVNII"
BASE COUNT         4074 a         1817 c         1799 g         4215 t
ORIGIN      
        1 gagtaagcat tttaactaaa gtaactataa ctaatgtagc tactaaaaaa acagttactt
       61 atcaaattgt tggtgaaaaa gaagccgatt taaaaatggg taaaatttct tcctcttctc
      121 caattggtaa gggtttaatt ggcaagcaaa taggagaaat tgccgaagtg caagcaccta
      181 atggtatatt gaaatttaaa gtagaagaaa ttgccattta aagcaatagc tttcaaaaaa
      241 ttctccaagc ctcaattatt aaattgaggc ttttttattg gtggtattgt aataaacctt
      301 gcattgggct tttgctaaaa ttgccaactt ctaattcata actgtggcaa agttctttaa
      361 ttacatcttt atatgtatat gctataccac cagtaaaatg aatagggtgt agccaacttt
      421 ctctatactt ataaatgtga ttttgaaaaa aatcatttaa gccgtcttca ataatatttt
      481 ctatcatata atgccctcta ttttctgata aaaaacttgc aaaacttgct aaatacctat
      541 tgggtgtatt gcttttataa attgagttta aaatagtagc cttgttagta ttgtaacttt
      601 tatcaaaatt tatagttagt tcttcatcaa aagtttgata taaataatac tgtaaaactt
      661 ttttacctaa ataagcacca cttccttcat ctcctaacac aaaacctaag gaagggttac
      721 ttttaataat tttctttcca tcgtaatagc aagaattgga gcctgttcct aatatacaag
      781 caataccttt ttggttacca cataaagatc ttgcagcagc caacaaatct gtatttactt
      841 ctattaatgc ccctttaaat atttgcttta aaatttgttt catttgttgg gcattcacct
      901 tattgccaat tccggttcca taaaaataaa cttttaaaac agtagtattt ttaattttac
      961 ttagtaattc ggttattaaa agttgtttta cattttcggc acttaaaaaa tataaactaa
     1021 aaccttgggt aactattttt tttactttgt tgttttcaag caaactccaa tcgcattttg
     1081 ttgcaccaca gtctgcaatt aatgtagcca taattttatt tttgtaaatt acagtaaaag
     1141 aaagtttcgg ttaaatatga aatataattc aatttaagga attttgttaa ggaataaaaa
     1201 cattttttgg ttgaactaaa aagaatttag gcaagttttg aagatatact gatattcgtg
     1261 ttttaataaa aagtactcac ctaaagtatc aaattttgat ggataataat aagaaacaaa
     1321 tttggctacc tctatttttt gctattgtaa tgatatttgg aatgttagtt ggttataatt
     1381 tacataaaca tactactagc agtaattttt ttagttcggg taaaacaaat tccatacaag
     1441 aggtagttag cttaattcaa aataaatatg ttgataaaat aaaaatagat agcatttctg
     1501 aagctaccat aaatgattta ctatctcatt tagacccaca ctctatttat ataccccaaa
     1561 atgatgtaca agcaattaat gatgatttaa ttggaaattt ttatggcatt ggtgtagaat
     1621 ttcaaatgtt tgaagatact gttacagtaa tttctgtagt taaaaacggg ccttcagaaa
     1681 aagcaggggt acaggctgga gataaaatta tttttgtaaa tgatactact aaaattgcag
     1741 gcgtaaaaat aaccacagat gtaataagaa atttattaag aggagaagaa aacagcaaag
     1801 taaatattac agtaataaga gatcagaaac aacaaaaaat aactattaca agaggctcaa
     1861 ttcctgtaag cactatagat gcatcttaca tgatagaacc acaaacaggt tttattagat
     1921 taaataaatt tggtgaaaaa acctatgagg aatttatgga gcaattagaa aaattgcaga
     1981 aagaaggaat gaaaaacctg attcttgatt taagaggaaa tacaggtgga ttactaaatg
     2041 aagctgtaga tattgcagat gaatttttat cgaacaataa attagttgta tataccgaag
     2101 gagcaaaatc tgccaggtat gaatttaagt gtaaaagaga tggtttgttt gaagaaggta
     2161 agttaattat tttagtggat gaaacaagtg caagtgctag tgaggtttta gctggtgcct
     2221 tacaagattg ggatagagca actattatag gaagaagaac ctttggaaaa ggattggtgc
     2281 agcaacaatt ttctttaagc aatgggggag cattaagatt aacagtatct aggtattata
     2341 cacctttagg cagaaatatt caaaaatctt ataccaataa atcaaaagag gaatatagga
     2401 gagatgcttt aggtattgaa ccacacgaaa aatatgaaaa tgaagattcg gcacacccta
     2461 aaccttcatt taaaacacct agtggtaaaa cagtttttgg tggaggtgga attttacctg
     2521 atatattagt tactacagac tctattacat taaccaacaa tattgctaaa atttatcata
     2581 ctaacttttt aaataaggtt gctcttaagt tatatattca aaacaaaaat tatttaagta
     2641 aaattaattc tgccgataag ttttatgctg aaaaattacc aaccgaaaat gatttatctt
     2701 tggttgaaaa aattgcaaaa tctgactcca ttagcttaca aaaattttca gaaaaagaaa
     2761 aggaaacctt acttttcagc attaaaaaac taattgctaa acaaatgttt ggaaataatg
     2821 ggtattatca acttgccaac aaaactgata aagtagtttt aaaggcaatg gaacaggtta
     2881 aacaataatt ttatattcat acagcaggtt tataaatttc agtatctaag aactattcta
     2941 tggaggaaaa tgaaaagata tacattctta aaaagaaaaa gtatatatat gggccctaca
     3001 attttgaaac cttaaaacaa aataatttaa aggcaggtga tatgatttgg tatgaaggct
     3061 taaaagattg ggtacctgta gaagaaatag aaggtttagc atcatttatt atttctgata
     3121 atacaaataa taatggatct aaaaagtcgt tttttaagaa aatattagga ttataatcta
     3181 tattttttca aataaatcag gtttaaggaa gaatatggaa atttatttag accaaattgt
     3241 ataatgtata aataattatt ataattttgt cagctaagta atcacctaaa cctataatta
     3301 agattttttg acttggaata aatttaaagc atgaaaagaa gcaaacagta tttattattt
     3361 attttattat ttggagcttt tattaactta ggaagttaca atagtgtttt ttcccaaatt
     3421 tttgaccata atggcaatac actaaatgga actggttcta caagcactaa tagtggaata
     3481 gacgaacctg tttttgaaga taactcaact tctactggta atactgctgc taatggtggt
     3541 ggttttgctg gtacaacttc tgttgatgca gatgatgatg atccaggtgg accaattgac
     3601 ccaatagatg atattccttt agatggtgga gttgcagtac tattagcagt tggtatagct
     3661 agtggttata atgcaagcag aaaaaaaatt atttaattca attgaaaaat aaattaatag
     3721 gggctgaatt ttaattcagc ccctattaat tttataatat ctacttattt tacttttgaa
     3781 tagtcaaaaa ctaattgaca caatgcttta acaccaacat ctaatttact atcatcaata
     3841 ataaaatctg gtgtatgatg aggtgcagct tgctctgatt ttatagtagc agacattcca
     3901 cctatataaa aaaagaaggc aggtgctttt tctccataaa aagaaaaatc ttctgcacca
     3961 gttacccaag ttctagaacg aacattattt ttaccagctg ctttctctaa tgatggcaac
     4021 atttttttaa ctagttgtgg agtgttgtag gttaccaaag ttttattttc tatagtaact
     4081 tcggctgttg catgatgcag ttttgcaata ccttctgcaa ttgcctttaa ttcttctaaa
     4141 accttaattc taatatcttt atccaaattt cttacagtac ccaacatttc tgcttcttca
     4201 ggtataatat taaatcttaa tccactatga atagcagcaa cagttattac aacaggagct
     4261 ttggttaaat cactgtttct gctaacaata ccttgaaaag cattaataat ttgagcagaa
     4321 acataaatag gatcaactcc taaccaaggc tgagagccat gagaactttt tcccttaact
     4381 tttattttaa acaaatcggc actagccata aatgcccctt ctttatattc taaatctcct
     4441 gcaggaatat cactttcaat atgcattcca aaaacaacat caacttttgg gttatccata
     4501 acaccttcct ttaccattaa actggcacca ccttcttcac cttctggtgc accttcttcg
     4561 gcaggttgaa aaataaatac aacagtacca ggaacagatg cttttaattt acttaaaacc
     4621 tctgcagcac ccattagcat tgctgtatga gcatcgtggc cacatgcatg cattacacca
     4681 gtattttgcc cattaaatgt ggttgttacc gttgatttat aaggcaaatt atttctttct
     4741 gttacaggca gagcatccat atctgctcta agtgctacaa caggccctgg ctttccacct
     4801 tttaaaatac ctaccacacc ggttttggca attccagttc taacttccaa acctaaattt
     4861 tttaaatgtt ctgctacttt tttactggta ttaaattctc ggttactcaa ttcaggaaat
     4921 tgatgaaagt gcctacgcca ttcaataact ttatttttta tttgtaaaac agcattgtta
     4981 acttgttccg tttcggtttg ggcaaagctt tttatattgc aaaaaaggag gcctaaaata
     5041 attaataaat atttcatgta tttacattta taaattacta aactaaaaaa aaaatataga
     5101 gtaacaaaat atgaaattga taatatacat gtgcaggggt ttctgttgct aaacatcttt
     5161 cttttttatt tgagtatttc acttttttac aactaaataa agcaatggta acattaaact
     5221 tttattagta ttttagcttt ttaaattata atctttaata aattacttca tttgaaaact
     5281 attctatcgg aacccgaaat agccattaca cttaaacggt tagcctatca aattattgaa
     5341 ggtgatgtgc agcttagaaa tactgttttc attggcatac agccaagagg tatttttttt
     5401 agtaatagaa tagttgcaga aataaaaaat attattgaac ctattaatgt tgaatatggc
     5461 aaactggata ttacttttta cagagatgat gtaagaaatg aattgcatat tgccaataaa
     5521 accgatataa attttagtat tgaaaataaa agggtagtac ttattgatga tgttttatgg
     5581 acaggaagaa ccatacgtgc tgctttagat gctttaatag attttggacg accagccaag
     5641 gttgaacttt gtgttttaat taatagaaga tttaacagag agttacctat acaaccagat
     5701 tatacaggaa aagcaataga tacttttatt tcggaaaaag tgaaagtagc atggaaggag
     5761 aatgatgaaa aagaagaagt gattttaatt cagtaaacta aaaatttgta caattatatt
     5821 caattgtttt aattttgtac tttaatgaaa ctatccacca aacacttgct tggtattaaa
     5881 gacctacaca aagaagatat tcaactaata ctttctacag caaataattt taaagatgtt
     5941 ttacaaaggc cagttaaaaa agttcctagc ttaagagata ttaccattgt aaacctgttt
     6001 tatgaaaaca gtaccagaac aagaatttct tttgaattgg cagaaaaaag attaagtgca
     6061 gatataatta attttactgc aagtggcagc agtgcagcta aaggagaaac gcttttagat
     6121 actgtgaata atattttaag tatgaaggtg gatatggtgg taatgaggca tagtgcaagc
     6181 ggagctccac attttttggc taaacatata cctgcagcca ttgtaaatgc aggagatggt
     6241 ataaacgaac atccaacaca agctttatta gatgcattta gtatgcaaga aaaatttgga
     6301 tccttagaag ggctgaaagt ggctattgtt ggcgatatta tgcatagtag ggttgcactt
     6361 agtaatattt atttgttaca aaaaatggga gccgaagttg tagtaactgg gccacccact
     6421 ttaattccta aatatatcac agaagctttg ggagtaaaag ttgaatacaa tattaaaaat
     6481 gctttgcaat ggtgtaatgt ggccaatgta ttaagaatac agttagaacg acaaaatcag
     6541 cctctatttt cttcgttgag agagtataat ttatcctatg gaatttcaaa aaaactgata
     6601 gacagtttaa ataaagaaat aacaattatg catcctggtc caataaatag aggggtagaa
     6661 ttagatagtg atattgcaga tggaagttcc tcaatcatct taaatcaggt agaaaatggg
     6721 gtagcagtta ggatggctgt tttatatttg ttagccaacc gagaaaatta attatagaga
     6781 gttcaaaatt tttatatgta aacttatgag tagtagaatt tgtctttttc ctggaacatt
     6841 tgaccctgtt acattagggc atgttgatat aattaataga gcattaccat tgtttgataa
     6901 aatttatatt gggataggag aaaatgctgc caaaaagcca atgtttactg cagagcaacg
     6961 attggtttgg tttaaagata tttttaataa tgagcctaaa attgagagtg ttatttttca
     7021 aggcttaacc attgaatatt gtaaaaaaat taacgccagg tttattttaa gaggcatacg
     7081 ttatgtaagc gattttgaat acgaaaaaac cattgccgat gctaacagaa ctttagatag
     7141 aacgatagaa accatatttt taactggcga acccaaatat acttctgttg catctactat
     7201 tgtaagagat atactaaaaa atggaggtga tgcatcgccg tttttacctg aagcagttat
     7261 taatagttta aaaaaataat tctcttttat tttcattgct atgatttggt ataataaaca
     7321 aattgctatt aaggattttg cacattgggg taagaatacc atgggagaat ttctagatat
     7381 tcaatttatt gaaattggtg ccgatttttt gaaagctacc atgcctgtta atacaaaaac
     7441 gcatcaaccc tatggcttat tacatggtgg tgcaagtgct acattggccg aaactgttgg
     7501 tagtgttgct tctgccttag taattaatac agaaaagcaa ctatgtgtag gcttagaaat
     7561 aaatgccaat catattcgta gtgtaaaaag tggtatagtt actgcaacag caaaaccctt
     7621 gcatttaggg gcaactactc atgtatggga tataaaaatt actgatgaaa atgaaaaatt
     7681 aatttgtgtt agtaggctta ctgttgccat actaaataaa aaaattacac attaaatcta
     7741 aagtgcatta catcgccatc ttgtacaata tattcttttc cttcaattct taatctgcca
     7801 ttttctctag cagcagcttc gttgccatat tttacaaaat catcgtaagc aataacttct
     7861 gctttaatga aacctttttc aaaatcggta tgtattacac ttgctgcttg tggggctttc
     7921 cacccttggt gaatggtcca agctcttact tcttgtacac cagcagtaaa gtaagtataa
     7981 aggtttaata ttttataagt agtttgaatt aacctatcta aagcaggctc tttcataccg
     8041 tattcttcca taaacatttg tttgtcctct tcagcttcca tttcggcaat ttgtgcttca
     8101 atattattgc acattacaat tacatctgct ccttcggcct ttgcatttat ttttaatgct
     8161 tcagaatact tattgcccgt gtgcatactt gcttcatcta cattagccac atatagcact
     8221 tgtttttcag ttagcaaaaa taaatcggat atggctactc tttcttcttt gcttaaacct
     8281 aagcttctta tactattacc tttttctaaa tgcagtttac attgcatcag tacatcatat
     8341 tcagccttca ttttaacatc tgttctcgaa agtttttcaa ctctttgtat tttcttttct
     8401 acactttcta aatcttttaa ctgtaattca atatcaatta tttctttatc accaacaggg
     8461 ttaatagctc cttcctctct taaaacattt tcatcttcaa aacaacgaat aacatgaata
     8521 atagcatcta cctctctaat atttgctaaa aatttattgc ctaatccttc tcctttactt
     8581 gcaccacgta ctaagccagc aatatctaca atttctaatt gagtgggtac aattctgttt
     8641 ggctttacca attctgctag tttagatagc ctataatctg gcacatctac taaaccagta
     8701 ttgggttcaa ttgtacaaaa tctataatta cttgcctgtg cttttgcact attacttaca
     8761 gcattaaata aagtactttt tcctacattg ggtaaaccaa caattcctgc ttttaatgac
     8821 ataatagttt agaattttat agcaccatta aataaaaaaa tcattaatga tgaatcaatt
     8881 tttaaaatgg ctgcaaatgt agcattgtaa ttaagtaaac caaactttaa gtacttataa
     8941 tgtgttaata gggtaaatta aaatttattt ttttgttctt tccattgtat aattatactt
     9001 tttatattgg aaaaactatt agttaaacat tgatttattt tattttcatc ggcccaaatt
     9061 acttcttcaa tatcctcttc tacttgtggt aaaaatggtt cgtttgttgt tatttgcatg
     9121 gcataccacc aggtttcttt tattacatgt tggctaatcc atttctcata atactcatga
     9181 taggttttac ccacaaaatt agttatagct acattctgta aaccggtttc ttcttgtact
     9241 tctctaatag cacattgttc tatactttct ccttcttcaa attttccttt tggtaaatcc
     9301 catttctttt ttctatatat taatagtaaa tcaccattat tattggatac taaaccaccg
     9361 gctgcaatta tttttttctt ttctttcatg ttgttgcaat ctacattaat tgaatgaaat
     9421 ttatttaata gtttgtagaa taacttttca ataattcatt atctgtatgc ttatagatgt
     9481 aaatattagc tttcctagtt tatctttgca gcatgacaaa tgaaaaagct gttgcagaaa
     9541 aacttttaca agtacaagct atacaattaa gccctaatgc accatttacc tgggccagtg
     9601 gctggaaaag tcccatttat tgcgataata gaaaggtttt atcttttccg tttattagag
     9661 attttgtaaa gagtgaaatg tgtaatatta tttttgaaaa atttgaagat atagaattgc
     9721 ttgcaggtgt agcaacagca ggtattgctt ggggtgcaat ggctgcagat caattaaaat
     9781 taccttatat atatgttcgt cccaaaccta aagagcatgg cttaggcaat caaatagagg
     9841 gattttatga aaaagggcaa aaggtattgg ttattgaaga tttaatatct accggtaaaa
     9901 gtagtatgca agttgttaat gttttaagaa accaagggtt agaagtggta ggtatagttt
     9961 ctatttttac ctatcaattt tctgaggctg ttcaattatt tgaagaaaat aatttacagc
    10021 atatatcttt aaccaactat actaatttaa ttgaccttgc tattgagaag cagttagtaa
    10081 atgcagagtt gcaaaatgtt ttattagatt ggagagataa tcctgctaca tggaatggca
    10141 tttaaccttt tgaaaactag gagtgcattg ttgcagcaac agtggtatca ataactctac
    10201 ttctatccgt tgttaacatt ctgctaggat acctgtttat cacaataaaa tcgtgtgttg
    10261 ccattatgat agctgtacca taatctttgg taatttgaaa taataattgc attatttcat
    10321 cacttgtttc tgggtctaaa tttccagtag gttcatctgc taaaattaat tttggcgagt
    10381 ttaataaagc ccttgcaata tctactcttt gttgttctcc tccactcatt tcataaggca
    10441 ttttaaaacc cttacttctt aaacctactt tatttaaaac atcatttatt ttttcttcta
    10501 ttaatttctc atctttccaa ccagtagctt ttaatacaaa ttttaaattt tcatgtacat
    10561 ttctatctgt cattaattga aagtcttgaa aaacaactcc taagtttctt cttaaaaatg
    10621 gtactttttt ccagttcata tcttttaaat caaagcctac cacattacaa cttccttctt
    10681 ttaagggcag ctcgccatat aatgttttta ataagctact ttttcctgta cctgttttgc
    10741 caacaagata aacaaattcg ccactttcta tagtaaagtt tacatcttgt aaaataaggc
    10801 tgttgccctg ataaatattt actcctctta attgaactac tgtttctgcc atgatattaa
    10861 ttttatttag caaagctaat tatacaaaat tgttttaaaa tgtcttttgt tttcacaaaa
    10921 tgcaataata tgttaagaaa gttattaaat aatatttact tctctctgta ataaaatact
    10981 aaatttattg taaacatcat taattatatc ttggctcaaa ttgtatattt caagccctgt
    11041 tgcatttcca taatttacta ataccaatgc ctgattggta tggcaaccaa catcattttt
    11101 tctaaagcct ttccaaccac aaaattctat taaccatgca gcaggaattt ttattgtatc
    11161 atctttatcg ttataacctg gtaattttgg gaattgctgt aatagtttgt ggtattgtgt
    11221 tttggctatt actgggtttt taaaaaaact acctgcattg ccaattattg ctgggtttgg
    11281 tagttttgta ctacgaatag aaataactgc atcggatatt gctttagcac ttaatgctgt
    11341 tactttcatt ttatctaact cttgttgtat agcaccatag ctgtggttaa atatattttt
    11401 tttatgaagt ttaaaagtaa cattaagaat gatgaattgg tttttgaatt tacctttaaa
    11461 aatactttct ctataaccaa atgcacaatc actgttatta aattttacaa ttgttttttc
    11521 ttgtatatgt aaagcttcta actcataaaa agtatctttt atttctacac cgtaggctcc
    11581 aatattttgc attgggcttg cccctacatt acctggtatt aagcttaagt tttctatacc
    11641 attataatta ttatgaatac agtataaaac aaattgatgc catacttctc ctgcagctac
    11701 tttaatataa tagtgttcat cgtcttcaca tatcaactct attcccttta attcattttt
    11761 taaaacaagc ccatcaaaat ttttagtaaa taaaatatta ctaccaccac caataattaa
    11821 agtagtatct gtattgtttt ctagtagttc aattaattgt tgaacagagt tgaaggcagc
    11881 aaataattta gcaataacat ttatt
//
LOCUS       DAOQHP010000021        11472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2797_length_11472_cov_4.014189, whole genome shotgun sequence.
ACCESSION   DAOQHP010000021 DAOQHP010000000
VERSION     DAOQHP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_11472_cov_4.014189"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1316)
                     /locus_tag="PLY81_01510"
     CDS             complement(159..1316)
                     /locus_tag="PLY81_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF28938.1"
                     /translation="MQAILVIDDEKSIRNVLKEILSNEGYKVEEAADGEDGFDKFSAK
                     DFDAVLCDIKMPKLDGIEFLQKATEIKPDVPIIIISGHGNIDTAVDAVKKGAFDYIAK
                     PPDLNRLLITIRNATDKKILVKETKTLKNKINKVQEIIGESAAILKIKETIEKVAITD
                     ARVLITGENGSGKELVAKWIHEKSNRNTGPLIEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQEGKITRVGGDKEINVDVRVV
                     AATNKNLIAEVEEKNFRLDLYHRLSVILIHVPSLNERREDIPLLVDIFLEDVANEYRQ
                     TKKAIDKKALEALQQHNWTGNIRELRNVIERLVILSSKNICLEDVKNYVVY"
     gene            1542..2285
                     /locus_tag="PLY81_01515"
     CDS             1542..2285
                     /locus_tag="PLY81_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNF28939.1"
                     /translation="MKILVCVSVTPDTTSKIAFKDGNSKFDDAGVQWIINPYDEWYSL
                     VRAIELKEKDASISIHLVTVGNKEAEAVIRKALALGGDEATRINAESNDSYFIAAQIA
                     DIANKGAYDLVFTGKETIDYNGSSIGGMVAELMNAPYISLATKFELNGNIATVTREIE
                     GGEEINEVVLPLVVSCQKGIAEQRIPNMRGIMAARTKTLHVVEPIVIDALTNVVNYEM
                     PPAKAGVKLVDADNVAELVRLLHEEAKAI"
     gene            2346..3311
                     /locus_tag="PLY81_01520"
     CDS             2346..3311
                     /locus_tag="PLY81_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNF28940.1"
                     /translation="MSVLIIIDKADGQIKKSSLEALTYGADVAKQLNTTAAALVLGTC
                     TDDLSTLGKYGVGKVYQVVEDSLNNLDAQVYAQVIASVTNQVGANVLIFSHNQTGKSV
                     AARVAANLKAGIVTGACALPDVSNGFVVKKTVFSGKAFANVNINSACKVISLNPNSYK
                     TVATEGSAIIETLNVSVPTAKVKVTGINKVSGEVPLTEAEIVVSGGRGLKGPENWGII
                     LDLAHVLGAATACSRPVGDAHWRPHHEHVGQTGIQIAPNLYIAVGISGAIQHLAGVNR
                     SKVIVVINKDAEAPFFKAADYGIVGDAFEVLPKLTAEIKKLKGIA"
     gene            3470..4576
                     /locus_tag="PLY81_01525"
     CDS             3470..4576
                     /locus_tag="PLY81_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF28941.1"
                     /translation="MKLVTKFIFTFSFLFFAWKSHAQFRKYSNEFLNIGAGARSLGMG
                     NVQVATANDATAGYWNPSGLIHIKKSPSLSVMHANYFAGIGKYDFAAIALPSKSSKRT
                     LAITFLRFAVDDIPNTLYLIEPDGSPNYNNISSFSSADYGVLLSYSQIAKQKENLFIS
                     YGGNVKIIHRKVGSFAKAWGFGIDAGIQIKKNKWLFGATIRDVTTTFNAWSFSFTEKE
                     KEVLYLTNNDIPVKSTEMTAPRLIAAAARNFKINKNINLLAEANLHFTFDGKRNTLIS
                     TNPISIDPNIGLEASLNNTIFLRAGVSNFQRALKDADTTNQKKVWIFQPSLGAGFKFG
                     NVGIDYAFTNLANQSNPLYTHIFCLRIDLVKKEK"
     gene            complement(4587..5858)
                     /locus_tag="PLY81_01530"
     CDS             complement(4587..5858)
                     /locus_tag="PLY81_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNF28942.1"
                     /translation="MNNFTPLAERLRPYELTDLIGQEHLTGNNSILRKAIEQGKIPSI
                     ILWGPPGVGKTTIANIIANKLHQPYYQLSAISSGVKDVREVIEKATAQKHAILFIDEI
                     HRFNKNQQDALLAAVEKGIITLIGATTENPSFEVNSALLSRAQVYVLKHLTEEDLMQL
                     LHIAIKKDVFLKEKNITLQETEALINLSSGDARKLLNLLELVVNSITTTNQQITITNK
                     QVIEIAQQKIALYDKQGEQHYDIVSAFIKSMRGSDPNGAIYWLARMIDAGEDIKFIAR
                     RMLVFASEDIGNANPNALLLANTTFDAVNKIGYPEGRIILSHCATYLASSVKSNASYV
                     AIDKALQTVQQQGNLPVPLHLRNAPTKLMKDLEYGKNYQYSHNGEGNFIEQEYLPDAL
                     KGTVFYSPGNNSRENELRNYLREKWKKKYNY"
     gene            complement(5952..8399)
                     /locus_tag="PLY81_01535"
     CDS             complement(5952..8399)
                     /locus_tag="PLY81_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF28943.1"
                     /translation="MKQIITLLSCIFFSAVTFAQQIKGNVKDEKSNGINAASVLLHNS
                     KDKSITKILVTNAEGAFKFMDIPKGEYYVEINSLGYVAKQSAVFLVDGNDVVLTDFIL
                     EKKSKQLDGVTVTSKKPPVEVKADKTIVNIEGSINAQGSDAFELVRKSPGVTIDKDDN
                     ITLSGKNGVVIYIDGKPSPLKGKDLADFLRTMNSNQIEAIELITNPSAKYDAAGNAGI
                     INIKLKKNKTFGTNGSFNAGYGIGVYGKYNTSLSLNHRNNKVNIFGTYSFNNSKNENN
                     MHFKRYLLDTLFNQSNVMTSSSQTNNIKLGMDYFINKQQTIGFIATGNFNNNDLNHNS
                     NTPITYIPTNTGNYILEAKNTNKGKRNNVTLNLNYKYTNAKGKELNVDADYGNYDNFN
                     NQLQPNYYYDFSGTNELSRKIYNIISPSKINLYTVKADYEQPLAKGKLSIGTKTSFVE
                     TKNNFERYDVFTNNKVLDTARSNNFNFKENINALYANYTKQIKNINVQIGLRVENTNA
                     TGNSIGYKKKNNNYVTYDSIFNRNYTNLFPSASITFNKNPMSQWSLSYSRRIDRPAYQ
                     DLNPFEFKLDEYTFQKGNTELTPQYTNSFNLTHSYKYKLITTLNYSYVNNVFTQLVDT
                     IEKSKSVLTEKNLASQNKVSINISYPIIYKWYSAFINTNFNYSHYKANLGIGRTIDLD
                     AFTFNIYMQNTFKLGKGYTAELSGWFNSPSIWEGTFKAKSMGNVDIGVQKQILKGKGN
                     LKLSVSDIFFISKWHVTSNFAGQYFDATGHWESRQFKINFNYRFGKSTVKSSRQRTTG
                     TEEENKRAQGGGGGIGQ"
     gene            complement(8547..8756)
                     /locus_tag="PLY81_01540"
     CDS             complement(8547..8756)
                     /locus_tag="PLY81_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF28944.1"
                     /translation="MKNLIKVERAKKNWTQEYLANKIGVSRQSMNAIEAGKFIPSTLL
                     ALKMAKVFGTSVESIFQLEKSDTLL"
     gene            complement(8753..9154)
                     /locus_tag="PLY81_01545"
     CDS             complement(8753..9154)
                     /locus_tag="PLY81_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28945.1"
                     /translation="MKINKKQINKLILFASILWAACYLIALNVIKEYTLSNEIKVLLS
                     ILPTITFGILIYFYITSINLMDEVEKRIHLEAAVFAFSTGLALIMTIGIVDTAFDLNK
                     EIFGTKKIFIYLFILYFIGVALSKNKYLFKK"
     gene            9330..10193
                     /locus_tag="PLY81_01550"
     CDS             9330..10193
                     /locus_tag="PLY81_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF28946.1"
                     /translation="MEKSIKPIQGFLALFVSLILLAAGVVIIGLYHEKVLLVIVGVIL
                     IITSFFILKGLMIINPNHSRVLNFFGKYVGSVKENGLFFINPFYSSLKVSLRAENLQG
                     QSLKVNDKMGNPIEIAAVIVWKVGDTYKATYEVENYNEYVSKQSEAAVRHLAVSFPYD
                     NLEDEKASITLREGGDKVNQILENELTERLAPAGIIILEARISHLAYAPEIAGAMLQR
                     QQATAIVAARTKIVEGAVGMVDLALKKLSEENIVELDDEKKAAMVSNLMVVLCGEKAA
                     TPILNTGTLYQ"
     gene            10207..10407
                     /locus_tag="PLY81_01555"
     CDS             10207..10407
                     /locus_tag="PLY81_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HNF28947.1"
                     /translation="MAEKKNFILRVDPETFSILEKWASDEFRSVNGQIEYLLNSAIIH
                     SGRRQLHTKKLAGNANTKIKKK"
     gene            10817..11284
                     /gene="mraZ"
                     /locus_tag="PLY81_01560"
     CDS             10817..11284
                     /gene="mraZ"
                     /locus_tag="PLY81_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNF28948.1"
                     /translation="MLNLLGEYEATIDSKGRFLIPGGLKKQLPDGESRLVISRGIEKC
                     LTLYPIKSWEPIVARIRLLNDFDPKVREFKRLFLGGATEVELDSAGRLLLPQQLKEYA
                     DLSKDIVLAADLDKINIWDASKYKQFFEDFSPEAFSNLAATVMANKSTNDIGN"
     gene            11288..>11472
                     /gene="mraW"
                     /locus_tag="PLY81_01565"
     CDS             11288..>11472
                     /gene="mraW"
                     /locus_tag="PLY81_01565"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNF28949.1"
                     /translation="MGTIKNKMMSKQIENTQHPNSNYHIPVLFNETIDTLNINPNGIY
                     VDCTYGGGGHSKGILKN"
BASE COUNT         3795 a         1690 c         1765 g         4222 t
ORIGIN      
        1 aatatattga atgatgtatt acagttatta attgatataa gaaaagaagc taaagccaac
       61 aaaaattttg ttaccagcga taccattcgc aacgaacttt tacgattggg tgtacaatta
      121 aaagatgaaa aagatggaaa tataagttgg agtatagact aatacaccac atagtttttt
      181 acatcctcta aacaaatatt ttttgaagaa agtataacca acctttcaat aacatttcta
      241 agttctctaa tattgcctgt ccagttatgt tgttgtaaag cttctaatgc ttttttatct
      301 atagcctttt tggtttgcct atattcatta gcaacatctt ctaaaaatat atccactaaa
      361 agaggtatat cttctcttct ttcatttaaa gaaggcacat gaattagtat tacactaagc
      421 ctatggtaca gatctaatct aaaatttttc tcctctactt ctgcaattaa atttttatta
      481 gttgctgcaa ctactcttac atcaacattt atttctttat caccacccac tcttgtaatt
      541 tttccttctt gtaaagccct taatacttta gcttgtgcac tcaggctcat atcgccaatt
      601 tcatctaaaa aaagggtgcc tccatttgct tgttcaaatt ttcctatacg ctgttttaca
      661 gcacttgtaa acgatccttt ttcgtgtcca aacaattcac tttctatcaa ttcagaaggg
      721 atggctgcac aatttacttc aattagtggc cctgtatttc tgttgctttt ttcatgtatc
      781 cattttgcaa caagttcttt accacttcca ttttcgcctg ttattaatac ccttgcatca
      841 gttatagcaa ctttttcaat tgtttcttta atttttaaaa tagctgcact ttctccaata
      901 atttcctgta ctttattaat tttatttttt aaagttttgg tttcttttac caatattttt
      961 ttatctgtag catttctaat agtaattaat aatctgttta aatctggtgg cttagcaata
     1021 taatcaaacg cacctttttt tactgcatct acagcagtat caatatttcc atgcccacta
     1081 ataataataa taggtacatc aggttttatt tcggttgctt tctgtaaaaa ttctattcca
     1141 tccaacttgg gcatttttat atcgcataaa actgcatcaa aatcttttgc tgaaaatttg
     1201 tcaaacccat cttctccatc tgctgcttct tctactttat agccttcatt acttaaaatt
     1261 tcttttaata cattgcgtat tgatttttca tcgtctatta ctaaaattgc ttgcataaaa
     1321 tttacttgaa atgaacatgc aatataagct aaacttgttt tttattcatt ttataaggta
     1381 aaggaattaa aaatttccat taagcctttt attacttact ttatttgaaa atatttatta
     1441 tttaaaattt ttttgcttaa cctttgccac aatttaaaca accttatatt tgcaatagtt
     1501 gtttatgcaa ctattaaatt aattttttaa ataattgtga tatgaaaatt ttagtttgtg
     1561 taagtgtaac accagatact acatcaaaaa ttgcatttaa agatggcaat tcaaagtttg
     1621 atgatgcagg tgttcaatgg ataattaatc cttatgatga gtggtactct ttagttcgtg
     1681 caattgagtt aaaagaaaaa gatgcttcaa taagcataca tttagttact gttggtaata
     1741 aagaagcaga agctgttata cgtaaggcat tggctttagg tggagatgaa gcgacacgta
     1801 ttaatgcaga aagtaatgat agctatttta ttgctgcaca aattgcagat attgctaaca
     1861 aaggagctta tgatttggtt tttacaggta aagaaactat tgattataat ggttcatcta
     1921 ttggtggtat ggttgcagaa ttaatgaatg caccatatat ttctttagct accaaatttg
     1981 aattaaatgg aaacatagca acagtaacca gagaaattga aggaggtgaa gaaataaatg
     2041 aagtggtatt gcctttagtt gttagctgcc aaaaaggtat agcagaacaa agaataccca
     2101 atatgagagg tattatggct gctcgtacaa aaactttaca tgtagtggag ccaattgtta
     2161 tagatgcatt aaccaatgtt gtaaactacg aaatgccacc agctaaagca ggtgttaaat
     2221 tagtagatgc tgataatgta gctgaattgg ttcgtttact tcacgaagaa gcaaaagcaa
     2281 tttaaaatgt attaaatatt catttcagaa tataatttct tattcaatta ttcaaatata
     2341 taaatatgtc agttttaata attatagata aagccgatgg gcaaatcaaa aaatcttctt
     2401 tagaagcatt aacctatggg gcagatgttg ccaaacaatt aaatactact gctgctgcat
     2461 tagttttagg tacatgtaca gatgatttat caactttagg taagtatggt gttggtaaag
     2521 tatatcaagt ggtagaggat agccttaata atttagatgc acaggtttat gctcaagtaa
     2581 ttgcaagtgt taccaatcaa gttggagcaa acgttttaat cttttctcac aaccaaacag
     2641 gtaaatcagt agctgccaga gttgctgcta atcttaaagc aggcattgta actggtgctt
     2701 gtgcattacc agatgtaagc aatggctttg ttgttaaaaa aactgttttt agtggtaaag
     2761 cttttgccaa tgtaaatatc aactctgctt gtaaagttat atctctaaat ccaaacagtt
     2821 ataaaactgt tgcaacagaa ggtagtgcaa taatagaaac gttaaatgta agtgtaccaa
     2881 ctgcaaaagt taaagtaact ggcataaata aagttagtgg agaagtgcct ttaactgaag
     2941 cagaaattgt ggtaagtgga ggaaggggtt taaaaggtcc agaaaattgg ggaattattt
     3001 tagaccttgc tcatgtattg ggtgctgcaa cagcttgtag caggcctgta ggggatgccc
     3061 attggagacc acaccatgaa catgtagggc aaacaggtat tcaaattgct ccaaacttgt
     3121 acattgctgt tggtattagt ggtgctattc aacatttagc tggtgtaaac agaagcaagg
     3181 taattgttgt gattaacaaa gatgcagaag ctccattttt taaagccgca gattatggta
     3241 ttgttggcga tgcttttgaa gtgctaccta agcttactgc tgaaattaaa aaactaaaag
     3301 ggatagcata aactacccaa caagtatagt ataaattggt tattttaaaa gaaaaactgg
     3361 ttattgaact cttaaattta aaattcaata atcttctttt gaaataacca attttttatt
     3421 aaagtaattc taatattttt acagcactat tcaagtacat taaaaattaa tgaaactggt
     3481 aaccaaattt attttcactt tttctttttt attttttgca tggaagtctc atgcacaatt
     3541 tagaaaatac tctaacgaat ttttaaatat tggagcaggt gctagaagcc ttggaatggg
     3601 taatgtacaa gtagccacag caaatgatgc aactgcaggt tattggaacc cttcaggttt
     3661 aattcatatc aaaaaatctc cttctttatc agttatgcat gcaaactatt ttgcaggtat
     3721 tggtaagtac gattttgcag ctatagcttt gccatctaaa tcaagcaaaa gaactttggc
     3781 aattactttt ttacgttttg ctgtagatga tattcctaac acattatatt taatagaacc
     3841 cgatggatca ccaaactata ataatatctc aagtttttca tcggcagatt atggtgtttt
     3901 gttaagctat tcgcaaattg ctaaacaaaa agaaaattta tttattagtt atggaggtaa
     3961 tgtaaaaatt attcatcgta aagttggcag ctttgctaaa gcatggggtt ttggaataga
     4021 tgctggaatt caaattaaaa aaaataaatg gctttttggt gcaacaataa gagatgttac
     4081 tacaactttt aatgcctggt ctttttcgtt tacagaaaaa gaaaaggaag tattatactt
     4141 aactaataat gatatacctg taaaaagtac ggaaatgact gctccaagat taattgctgc
     4201 agcggctcgt aattttaaaa tcaataaaaa tatcaattta ttagccgaag ctaatttgca
     4261 ttttactttt gatggtaaaa gaaatacatt aattagtacc aaccccataa gtattgaccc
     4321 aaatattgga ttagaagcaa gtttaaacaa taccattttt ttaagggctg gagtttccaa
     4381 ttttcaaagg gctttgaaag atgcagatac aaccaatcaa aaaaaagttt ggatttttca
     4441 accaagtttg ggtgcagggt ttaagtttgg aaatgtaggt attgattatg cttttaccaa
     4501 tttagctaac caaagtaatc ctttatacac acatatattt tgtttgagaa tagatttagt
     4561 aaaaaaagaa aagtagttct tatagactaa taattatatt tctttttcca tttttctctt
     4621 aaataatttc ttaattcgtt ttctctacta ttattgcctg ggctataaaa tactgttcct
     4681 tttaaggcat ctggtaaata ctcttgctca ataaaatttc cttcgccgtt atgagaatat
     4741 tgatagtttt tcccatattc taaatctttc ataagcttag taggggcatt tcttaaatgc
     4801 aaaggcacag gtaaattgcc ttgctgttgt actgtttgca aagctttatc tattgctaca
     4861 taagaggcat tacttttaac agaagatgct aaataagtag cacaatggct tagaataatt
     4921 cttccttctg gatatccaat tttattcact gcatcaaatg tagtattggc taataataaa
     4981 gcatttggat tggcattgcc tatatcttca cttgcaaaaa caagcattct tcgggcaata
     5041 aattttatat cttctccagc atcaatcatt ctggctaacc aataaattgc accatttggg
     5101 tcgctgccac gcatactttt aataaaggca gaaacgatat cgtaatgttg ttctccttgc
     5161 ttatcatata aagcaatttt ttgctgagca atttcaatta cttgtttatt ggttattgtt
     5221 atttgctgat tggttgtagt tattgagtta actacaagct ctaataaatt taataatttt
     5281 cttgcatcac cactacttaa gttaatgagt gcttctgttt cttgcagtgt aatatttttt
     5341 tctttaagaa agacatcttt ttttatagca atatgtaata gttgcattaa gtcttcttct
     5401 gttaaatgtt ttaatacata tacttgtgcc ctgctcagta aagcactatt tacttcaaag
     5461 gagggatttt cggtggtggc accaattaat gttattattc ctttttctac tgctgctaat
     5521 aaggcatctt gctggttttt attaaaacga tgaatttcat caataaataa aattgcatgt
     5581 ttttgtgctg ttgctttttc tattacttct cttacatctt taactccact acttatggca
     5641 cttaattgat aatatggttg atgtaattta ttagcaataa tatttgcaat ggttgtttta
     5701 ccaacacctg gtggtcccca taaaataatg gatggaattt taccttgctc aatagccttt
     5761 cttaaaatac tattattgcc tgttaaatgt tcttgaccaa ttaaatctgt taattcataa
     5821 ggtcttaatc tttctgctaa tggtgtaaaa ttattcatag ctgaaaggta gaatttattt
     5881 tagttattaa aaaaaaatgc caccaacaag attgttgatg acattcatta aaactaaacc
     5941 tgagaaaaaa tttattggcc aattccaccg ccaccacctt gtgctcgttt attttcttct
     6001 tctgttcctg ttgttctttg ccttgagctt tttactgtgc tttttccaaa acggtaatta
     6061 aaatttatct taaactgtct gctttcccaa tggccagtag cgtcaaaata ttggcctgca
     6121 aaattgctgg ttacatgcca tttagagata aagaatatat cacttacact aagctttaag
     6181 tttccttttc cttttaatat ttgcttttgt actccaatat ctacattacc cattgattta
     6241 gctttaaacg taccttccca aatactaggt gagttaaacc aaccacttaa ttcagcagta
     6301 taacccttgc caagtttaaa tgtattttgc atgtaaatat taaatgtaaa agcatctaaa
     6361 tctatggttc tacctatacc taaatttgct ttgtaatgtg agtaattgaa attggtattt
     6421 ataaatgcac tataccattt gtaaataatg ggataactga tattaatgct tactttattt
     6481 tggcttgcta aattcttttc ggtaagtaca cttttgcttt tttcaatggt atctactaat
     6541 tgtgtaaaca cgttatttac atagctatag tttaaggttg taattaactt atacttataa
     6601 ctatgtgtta aattaaaact gtttgtatat tgaggagtta gctctgtatt gcctttttga
     6661 aatgtgtact catctaactt aaattcaaat gggtttaaat cttgatatgc tggtctatct
     6721 attcttctac tataacttaa actccattgg ctcatagggt ttttattgaa agtaatgctt
     6781 gcactaggaa acaagttggt atagtttctg ttaaatatgc tatcgtaagt tacatagtta
     6841 ttgtttttct ttttataacc aattgaattt ccagttgcgt tagtattttc tactcttaaa
     6901 ccaatttgta cattaatatt ttttatttgc tttgtataat ttgcataaag ggcattaata
     6961 ttttctttaa aattaaaatt attacttctt gcagtatcta atactttgtt gttggtaaaa
     7021 acatcgtatc tctcaaaatt attttttgtt tcaacgaaag atgtttttgt tcctatactt
     7081 aattttcctt ttgctaaagg ctgttcataa tctgctttta cagtatataa attaattttg
     7141 gaaggggaaa ttatgttata aatttttctg gaaagttcgt tagttccact aaagtcataa
     7201 tagtaatttg gttgtaactg attattgaaa ttatcataat taccataatc tgcatcaaca
     7261 tttaattctt taccttttgc attcgtgtat ttgtaattta aatttaatgt aacattattt
     7321 ctttttcctt tattggtatt tttagcttct agaatataat tgcctgtgtt agtaggtata
     7381 taagtaattg gtgtattgct gttgtgattt aaatcgttat tattaaaatt acccgttgca
     7441 ataaatccaa tagtttgttg cttgtttata aaatagtcca tacctaattt tatgttattg
     7501 gtttgactag aacttgtcat aacattggat tgattaaata aagtatctaa cagatatctt
     7561 ttaaagtgca tattgttttc attcttacta ttattaaaac tatatgtacc aaaaatgttt
     7621 actttattat ttctgtgatt taggcttaag cttgtattgt attttccata aacaccaata
     7681 ccatagcctg cattaaaact accattggtt ccaaatgttt tattcttttt aagttttata
     7741 tttattatac cagcattacc tgcagcatcg tattttgcag atgggttagt aattaattct
     7801 atggcttcta tttggtttga attcattgta cgtaaaaaat ctgccaaatc ttttcctttt
     7861 aaaggagagg gtttaccatc aatataaatt acaacaccgt ttttaccgct taaagtaata
     7921 ttatcatctt tgtctattgt tacacctggt gattttctaa ctaattcaaa ggcatctgaa
     7981 ccttgggcat taatacttcc ttctatgttt acaattgttt tgtctgcctt tacctcaaca
     8041 ggaggttttt ttgaagttac ggttacacca tctaattgtt ttgatttttt ttctagaata
     8101 aaatctgtta aaacaacatc gtttccatca actaaaaata cagcagattg tttagcaaca
     8161 tagccaagag aatttatttc tacataatac tctcctttag gtatatccat aaacttaaat
     8221 gcaccttctg cattggttac taaaatttta gtaatagatt tatcttttga gttatgtaat
     8281 aatacgctgg cagcattgat gccattgctt ttttcatctt ttacattgcc ttttatttgt
     8341 tgggcaaatg taactgcact aaaaaaaatg caggataata atgtaattat ttgtttcata
     8401 taatttgata gtttagagtt tataatttga aatcaaaact gaaaattcta aactattttt
     8461 tctattagat tcttaccaat ggaaataaaa aacctttgag cggtagaagc tatatagaac
     8521 aatgaaacga ggaataattt aaaaagttac aaaagcgtat cacttttttc tagttgaaaa
     8581 atactttcaa cagaggttcc aaagaccttt gccattttaa gtgcaagaag tgtagaaggt
     8641 ataaattttc cggcttctat tgcattcatt gattgccttg aaacacctat tttattggct
     8701 aaatactctt gtgtccagtt cttttttgct cgttctactt taattaaatt tttcattttt
     8761 taaacaagta tttgttttta gataaggcaa caccaataaa atataaaata aataaataga
     8821 taaatatttt tttggttcca aatatttctt tgtttaaatc aaatgctgta tctacaatac
     8881 caatggtcat tattaatgct aaacctgtag aaaatgcaaa tactgcagct tctaaatgaa
     8941 ttcttttttc cacttcatcc attaagttaa tgctggtgat ataaaagtag attaatatgc
     9001 caaatgtgat agtaggaaga atggatagta atacttttat ttcattgctt aatgtatatt
     9061 ctttaataac atttaatgct attaaataac atgcagccca taaaatactt gcaaataaaa
     9121 tgagcttgtt gatttgtttt ttgttaatct tcataatgta aatgatttaa tttttcacaa
     9181 agataagtat tttttacaaa atgtaaaata aactttacaa aatgtatata tttcttgtca
     9241 taaatttttt tttacttttt caaataaaat ttggctactc tataaaacta atatactttt
     9301 gatattataa taatatcaaa atgaattgta tggaaaaatc tattaaacct atacaaggct
     9361 ttttagcctt gtttgtatcc ttaattttac tagctgctgg agtagtaata attggcttgt
     9421 atcacgaaaa agttttacta gttattgttg gtgtaatttt aataattacc tcgtttttta
     9481 ttctgaaagg gctaatgata attaatccta atcactccag agttttaaat ttttttggta
     9541 aatatgttgg tagcgtaaaa gagaatggat tgttttttat aaacccattt tactcctcct
     9601 taaaagtaag tttaagagcc gaaaacctgc aaggacaatc attaaaagta aatgataaaa
     9661 tgggtaatcc tattgaaatt gcagcagtaa ttgtttggaa agttggtgat acttacaaag
     9721 caacctatga agtagaaaat tataacgaat atgtaagtaa acaaagtgag gctgcagtaa
     9781 ggcatttggc cgtaagtttc ccgtatgata atttagaaga tgaaaaggca tctattactt
     9841 taagagaagg aggagataaa gtaaatcaga ttttagaaaa tgaattaaca gaaagattag
     9901 cacctgctgg aattataatt ttagaagcaa gaattagtca tttggcatat gcaccagaaa
     9961 ttgcaggagc tatgctacaa cgccaacaag ctacagcaat agttgcagct cgtacaaaaa
    10021 ttgtagaagg tgccgtaggc atggttgatt tggcgttaaa aaaattatcg gaagaaaata
    10081 ttgtagaatt agacgatgaa aagaaagctg caatggtgag caaccttatg gtagttttat
    10141 gtggcgaaaa agcagccaca cctatattaa atacaggtac tctctatcaa taataaatat
    10201 tttattttgg cagaaaagaa aaattttatt ttaagggtag atccagaaac cttttctatt
    10261 cttgaaaaat gggcttctga tgaatttaga agtgtaaatg ggcaaataga atatttgcta
    10321 aatagtgcaa ttatacattc tggcagaagg caacttcaca ctaagaaatt ggcaggcaat
    10381 gccaatacaa agatcaaaaa gaaataaatt atacctctat ataaatcatg tagaggtatt
    10441 tttttattca atttatagat tttttctttt accaaactgt tccttaacta ctactataag
    10501 ctatgtatta atctttagaa atgcagtata aaatacattt ttccacaaat tacattaggt
    10561 attttgggtt ttaatatgct gggtaccgca ttttgtttag tttttcaaac attcccacac
    10621 cttattaagg cttattcaca cttattaaca cttcattaag ggtgttattg ctaagaatct
    10681 tctgaagtgc tttatttaca agcatcctgt taaaatatta tttgtggata aatacaaaca
    10741 ctataattgt gttttaaagt gggaaaatgt gttattttgt gtcaataaat gttaaaaatt
    10801 atctcaaacc attaaaatgc taaatcttct tggtgaatat gaagcaacga ttgactcaaa
    10861 aggtcgtttc cttattccag gaggcttaaa aaaacagctt ccggatgggg aaagccgttt
    10921 agttattagc cgtggtattg agaaatgttt aaccctatat ccaataaaaa gttgggagcc
    10981 cattgttgca cgtattagat tattaaatga ttttgaccca aaagtaagag aatttaaacg
    11041 cttgttttta ggtggtgcta cagaggttga attagatagt gcaggaagat tattgttacc
    11101 ccaacaatta aaagaatatg ccgatctcag caaagacatt gttttggctg cagatttaga
    11161 caaaataaat atctgggatg caagtaagta caaacagttc tttgaggatt tttcaccaga
    11221 agcatttagc aatttagctg ctactgttat ggccaataaa agtacaaatg atataggtaa
    11281 ttagaatttg ggaacaataa aaaataaaat gatgagtaag caaatagaaa acactcaaca
    11341 tccaaattca aactaccata ttcctgttct ttttaatgaa acaattgata cattaaatat
    11401 taatccaaat ggaatatatg ttgattgtac gtatggagga ggtggccata gcaaaggaat
    11461 attaaaaaat tt
//
LOCUS       DAOQHP010000022        11262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_2865_length_11262_cov_3.736504, whole genome shotgun sequence.
ACCESSION   DAOQHP010000022 DAOQHP010000000
VERSION     DAOQHP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_11262_cov_3.736504"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..766
                     /locus_tag="PLY81_01570"
     CDS             353..766
                     /locus_tag="PLY81_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNF28950.1"
                     /translation="MEYSKLISVTGLPGLFEMVGSKTDGAIVRNLEDKTTKFVSSRIH
                     NFSHLESIEVYTLGENVNLIDIFKAIESSNEPLPNDKDAKAVKAFIEKVYPEIDVERV
                     YASDMKKMVKWYSVLKANNVELKLSEQAEDAEITE"
     gene            853..3747
                     /locus_tag="PLY81_01575"
     CDS             853..3747
                     /locus_tag="PLY81_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNF28951.1"
                     /translation="MNCSKYFSIVLLLSFSLYSNAQKFSEKSANATKWADSIMHTLTN
                     EQKIAQLMIVRAHSNLGEEHIKKVTELITQYNIGGLCFFQGGPIRQANLTNYYQSIAK
                     TPLMICIDAEWGLGMRLDSVIKFPRQLMLGAVQDVELIYQVGKAIGLQCKRLGIHVNY
                     APVIDINNNANNPVINDRSFGEDKNKVALFGTKYMQGMQDVGVMACAKHFPGHGDVNV
                     DSHYDLPVINKTKQQLDSLELYPFKQLIKAGVGSIMIAHLFIPSIDSTPNLATSLSKK
                     SVTELLRKELGYNGITFTDGLEMQGVKKFFPDDEVAVQSLVAGNDLLCLPENIQNSIS
                     KIITAINNGRLTWQEIDEKVYRVLIAKYNLGLHKITPIETENITADIDKYTNQLRQEI
                     SKKAITVLKLTNKKILPLFSKRKIAYIGVGINQMNSFAAEVKEIYNAKIFLLDYTDTN
                     NIAQIKKQLKKYKTIIVGLHNFSRRPANNFGINTPTIQFIQYLAEKNNALIFAFGNPY
                     VIKNFCEAKNLIACYEDDNITQLTAANLLQGKFDAKGKLPVTVCNDFKFGDGITYNHY
                     LPFSKKFNSNKFSIIDTIALNAIHQHATPGLSLLVAKKNEIIYYKNFGFTDSTQLHNV
                     TNNTIYDVASITKVAAVTVAIMKLYDEGKIELEKPISNYLPYTLHTNKANITIKDLLL
                     HQSGLVPFISFYKQTIDTVTHKPLQGFFNTSLTEMFTTEVAANMFLVKNFKEKIIKSI
                     LDSELNQKGKYVYSDNNFILLAFIIEAITQKTLNEYVQEIFYKPLQMTTTVFNAQKHF
                     VLNNIAPTELDKYFRQQLIQGYVHDEGAAMLGGVAGNAGLFSNAYDLLQLFQMLLNKG
                     SLNGVQYISEKTINLFTSYQSNESRRGLGFDKPLLNNAEAENPYPCYSISANSFGHTG
                     FTGTCVWVDADKKLIFIFLSNRIHPSRENKLLNTLNVRSNLQETFYNILF"
     gene            3850..5565
                     /locus_tag="PLY81_01580"
     CDS             3850..5565
                     /locus_tag="PLY81_01580"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HNF28952.1"
                     /translation="MNKIISTLSLLVTIIVFTTSCGKKAPSEAKYIPKDATSIVVINQ
                     KTLEEKLKSGNITLDSFINKMLDTKDSANAASLKKWQDFKNSGIDLDNNLYFFVVQKG
                     SISKGLTTAVNIMAGLKDQAKFEKYIKEQKEFNAKNLIQENNFSYLSSIDTDLSLSWN
                     KEIAIATFYKRDIKGSYDSVGNYIEPDKSETTKDKNEEVKRYFNLKENESVASVAYFN
                     DMFKTKADGYIFGSTNSMLGFLASTPLNIPKVEELLKDNYTASTFNFENGKIEVNNTT
                     YTNPMLSSILKKYSGSSVNTHLAEFFPTQNLNGTILISFKPEMFDGLLKELEIKGMLE
                     AFLAQQGLTSADLFKLLSGEINIAMSDFVIENKEVTQKLYDGSTYTYNKTVPNFKLIA
                     TAPIGDKAAFTKIMDKAVENNLLAKTSTGYTSGEMLASSGMYFMADEKQIVIASSQDF
                     YTTYLNNKTKSNLNSNIIADFKGKSMAAFIDINSIIKGFTPTAKDTTISNKVLAMVNT
                     TFKNFIATTENFKDNSFKGKVTLNMVNEKQNSLVSIINLIDEASKAIKQTTNSTTNSI
                     DENTF"
     gene            5704..6549
                     /locus_tag="PLY81_01585"
     CDS             5704..6549
                     /locus_tag="PLY81_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNF28953.1"
                     /translation="MKKIALFAFALYTVCSVSAQIKMPAPSPSQNISQDFGLGKIELS
                     YSRPSIKGRKIFKEGSELVPLNNVWRTGANGATTLKFTDEVVINGVTIAPGKYGLLSI
                     PGSTNWTLIITKDTTVTSPAAYKQENDIVRIVATTTKMKQKVETFTMQFANIQPESCE
                     LHLMWDKTAISLPITTKIIDRVRTQTENALAAEKVSGNTYYAAANFYYEYDKNYNKAL
                     TCVTKAIEINPKFFWMYLLKAKIEKALGDKVAAKQSAEKCKEIAGQQNNTDYVRMSDE
                     LIKSL"
     gene            6718..6855
                     /locus_tag="PLY81_01590"
     CDS             6718..6855
                     /locus_tag="PLY81_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28954.1"
                     /translation="MDIINLIDRKEILFFANRTSGTFCFAANAQANPKVIQKEPLLPT
                     L"
     gene            6870..8042
                     /locus_tag="PLY81_01595"
     CDS             6870..8042
                     /locus_tag="PLY81_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28955.1"
                     /translation="MEKSYKGNFYKNLNQSFLKPRYLLFIEDEINPTLNRYIIENYEK
                     ISKAVESKEMEFIYFPFISKATNEFIFSLTDFLKYKHPILYSFNAKEIKNIIAFLISS
                     TEPLEFYSIVLKEIGLSRIEHSGLIRNFEGAPEFDSKKKFSFVYPSGSTYAEYEMFFE
                     SFISQLLIPSKIPQTIFYQLAEESKPNSYDADWYFSKESNIASDELKLLIDSFQKEGK
                     YALLADAVMYMLSSLKDNQPEFIKKVKPIIEHKQLLDTDVILSKIMVDNHYNIFLPDF
                     GNREIKMHALPKSLYLLFLRYPNGIRFKELYEYKKELLEIYNKVTNKYEKEEIVRAID
                     DLVDMTKNSINEKCSRIREAFVSIMDDNTAKHYYITGERGKPKKILLPNKLIDIRY"
     gene            8039..9139
                     /locus_tag="PLY81_01600"
     CDS             8039..9139
                     /locus_tag="PLY81_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28956.1"
                     /translation="MMKVHLIKTPEYSPEDFRDVSELLNSFDGALEFVASEYEFDKEQ
                     FPFLQKFYPDFKFKYESDIKKLNFNKELGLPLSWRELFSLCDFYRTTFNINETDFIIV
                     LTIRRNALNWFSHCENKNAFVHTGDWEYFTDANPKYPVAHQIVENVMQSLMKLDTVNI
                     PNINVHTDSRGCMNDFCQNKGQIILKLRTADICPDCIEKIQEENIDHNIVNQVLKIFE
                     GIRNELLFKQKFKKQVTPVPITINERNQIILPQLGNLEIRLNPLFKTLYIFYLRHTEG
                     VRLNELNDFRSELLSLYRRLSVADNNQTIELRITDLVNPIGGSFSQKKSKLNKIITDL
                     LGEPLAKFYRIEGEPGEPFKINLPNKLIDIRY"
     gene            9235..9663
                     /locus_tag="PLY81_01605"
     CDS             9235..9663
                     /locus_tag="PLY81_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28957.1"
                     /translation="MAKKKRTSKMIYQETETLRELAKSWNNLDTSFIEKHLSDDFVYE
                     SQWVLTPIEGKREFLNYLQSKFQAIKSTMQKETMIVSAELASHPAMQNRPIIVLTQIT
                     KEGIRQVSLLIKIENDKLKRIDVCFIPDPSEAELTGEFPK"
     gene            9969..10547
                     /locus_tag="PLY81_01610"
     CDS             9969..10547
                     /locus_tag="PLY81_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28958.1"
                     /translation="MSIIEKLFGGKAKTENPVTTITEPQSTVAEQPENIPPVDLFIDK
                     EVPQQEQQQVAESKSRITLFLDSNYHSIGINDGYEYHSQETLETGKKKIRAEFQLIVD
                     QAIQEKSERRLQLRMMIVNVEKIAEETKRNLELTIEELNSSIALLQKQKELSAENEGW
                     VMNAIHPYHQGYVQGVNIRIESENFLNPIKNI"
     gene            10661..>11262
                     /locus_tag="PLY81_01615"
     CDS             10661..>11262
                     /locus_tag="PLY81_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HNF28959.1"
                     /translation="MIPVILWSINSYLLVSHVLEGSFFAAIFTAILAGFIIFLIERAI
                     IMSNGSKPIFWFRILLGFIVASLGSVSMDEVIFKHDIDNQVAYYKQAEIDSAVHKVER
                     DYQNQISMQQSIVKQKGSEWNQSLKDAKSDADGTGGSRQRGVGKISTLKLNIAGKQEV
                     DYNTENNKLTSLIASVDTAKAHAKEKAETDFNGNALLIRIR"
BASE COUNT         4307 a         1723 c         1794 g         3438 t
ORIGIN      
        1 taaaaaaaga gtttactcat tgaaatattt atatttaatg cttaattata tttttagttt
       61 aagtgtaaca aaaaatattc tacaaatgta ggttcattaa tataattgct ttcgttatct
      121 atacaactaa atagtgaaat gaatttttaa aaatacccaa ttaagggtat tgctgtattt
      181 aaataaatgt tgtttcattg catttgaaga agtacttcaa ttcttattca gggggttcta
      241 aggggtagct gatggttaat accatcagct ttttttattt acaaaactta taacttttat
      301 actattctgt aatataaaaa tacctttatt ttgcagccat taaaattaaa taatggaata
      361 tagtaaatta atttcagtta cgggtttgcc aggtttattt gaaatggtag gtagtaaaac
      421 agatggagct attgtaagaa atttagaaga caaaacaacc aaatttgtaa gtagccgtat
      481 tcataatttt tctcatttag aaagtataga agtttataca cttggtgaaa atgtaaattt
      541 gattgatatt tttaaagcaa ttgaaagcag taatgaacca ttacctaacg ataaagatgc
      601 aaaagcagtt aaagcattta tcgaaaaagt ttatcctgaa atagatgtag aaagagtgta
      661 tgccagcgat atgaaaaaaa tggtaaaatg gtatagcgtt ttaaaagcta ataatgtaga
      721 acttaaatta agtgaacaag ccgaagatgc agaaataacc gaataatttt taaatacaaa
      781 ttaactttta atagccacat tcatctgtgg cttttttgtt tgtacattag caaatatttt
      841 taaattaaat taatgaattg tagtaaatat tttagtattg tattactctt aagcttttcg
      901 ttgtatagta atgctcaaaa attttctgag aaatcagcaa atgcaaccaa atgggcagat
      961 agtataatgc atacattaac caatgagcaa aaaattgcac agctaatgat agttagagct
     1021 catagcaact taggagaaga acacattaaa aaagttactg agttaataac acaatacaat
     1081 ataggtggat tatgtttttt tcaaggtggc cctattaggc aagctaacct taccaactac
     1141 taccaatcta ttgccaaaac acccttaatg atttgtatag atgcagaatg gggcttaggc
     1201 atgaggctgg atagtgttat aaaatttcct cgtcagctta tgcttggtgc tgtacaagat
     1261 gttgaattaa tataccaggt aggaaaagca attggcttac aatgcaaacg tttaggtatt
     1321 catgtaaatt atgctcctgt aattgacatt aacaataatg ccaacaatcc tgtaattaat
     1381 gatagaagct ttggagaaga taaaaataag gttgccctgt ttggtacaaa atatatgcaa
     1441 ggaatgcaag atgttggtgt aatggcttgt gcaaaacatt ttccaggaca tggtgatgta
     1501 aatgttgaca gccattacga tttacctgta attaataaaa ctaaacaaca attagatagc
     1561 ctagagttat acccttttaa acaattaata aaagctggtg ttggcagtat aatgatagcc
     1621 catttattta tcccatctat agacagtact ccaaatttgg caacctctct ttctaaaaaa
     1681 agtgttacag aacttttaag aaaagagtta ggatataatg gaattacttt tactgatggt
     1741 ttagaaatgc aaggtgtaaa aaagtttttt ccagatgatg aagtagctgt acaaagctta
     1801 gttgctggta atgatttgct ttgcttgcca gaaaatattc aaaatagcat atcaaaaatt
     1861 atcactgcaa ttaataacgg aagattgacc tggcaagaaa tagatgaaaa agtttacagg
     1921 gttttaattg caaaatataa tttaggctta cataaaataa cacctattga aacagaaaat
     1981 attactgctg atattgacaa atataccaac caactaagac aagaaattag taaaaaagca
     2041 atcaccgttt taaaactaac caataaaaaa atattacccc tttttagcaa aagaaaaatt
     2101 gcttatatag gagttggcat taaccaaatg aacagctttg cagccgaagt aaaagagata
     2161 tacaatgcta aaatattttt acttgattat accgatacga acaatattgc acaaataaaa
     2221 aaacaattaa aaaagtataa aactataatt gttggcttac ataattttag cagaaggcca
     2281 gcaaacaatt ttggcattaa tacaccaacc attcaattta ttcaatacct ggctgaaaaa
     2341 aataatgctt taatttttgc ttttggtaac ccttatgtaa ttaaaaattt ttgtgaggct
     2401 aaaaacttaa ttgcttgtta cgaagatgac aatataacac agttaacagc agctaactta
     2461 ctgcaaggca agtttgatgc aaaaggtaaa ttgcctgtaa cagtttgcaa tgattttaag
     2521 tttggagatg gaattaccta taaccactat cttccatttt ctaaaaaatt taactcaaat
     2581 aagttttcta ttattgatac cattgcttta aatgctatcc atcagcatgc aacaccaggc
     2641 ttatctttat tagttgctaa aaaaaatgaa ataatttatt ataaaaattt tggttttact
     2701 gattccactc aactacacaa tgttaccaac aatactattt acgatgtagc ctccattacc
     2761 aaagtagcag cagtaactgt tgccattatg aaattatatg atgaaggaaa aatagagtta
     2821 gaaaaaccaa ttagcaatta tttaccttac actttacaca ccaataaagc caatataaca
     2881 attaaagatt tgttgttgca ccaatctggg ttagtgcctt ttatttcttt ttacaaacaa
     2941 accatagata ccgttaccca caagccctta caaggttttt ttaatacttc cttaaccgaa
     3001 atgtttacta ccgaagtagc agcaaatatg tttttggtaa aaaattttaa agagaagatt
     3061 attaaaagca ttttagacag tgaattaaac caaaaaggga aatatgttta tagcgataac
     3121 aattttattc tattagcatt tattattgaa gccataacac aaaaaacttt gaatgaatat
     3181 gtacaagaaa tattttataa gccattacaa atgactacca cagtatttaa tgcacaaaaa
     3241 cattttgtat taaataatat tgcacctact gaactagata aatactttag acagcaacta
     3301 atacaaggtt atgtacacga tgaaggagca gcaatgttag gtggtgttgc agggaatgct
     3361 ggcttattta gcaatgctta cgatttgcta caactatttc aaatgctttt aaataaaggc
     3421 agcttaaatg gtgtgcagta tatttcagaa aaaacaatta atttatttac atcctatcaa
     3481 agcaatgaaa gcagaagagg attagggttt gataaaccat tgttgaataa tgcagaagcc
     3541 gaaaacccct atccttgtta ttctatttcg gcaaactctt ttggccacac aggttttaca
     3601 ggtacatgtg tatgggtaga tgctgacaag aaattaatat tcatcttcct ttctaacaga
     3661 atacacccaa gcagagaaaa taaactttta aatacactaa atgttagaag caatttgcaa
     3721 gaaacttttt acaatatttt attttgaaat aaggattctt ccttttaaac attaattttt
     3781 aaaatttata cttctttact gtattgttta ttgtacattt tttatattcg tatccaaaat
     3841 aagttatcaa tgaataaaat aatttctaca ctatcgttgt tagtaacaat aatagtattt
     3901 acaacttctt gtggtaaaaa agctccatcc gaagcaaagt atattcctaa agatgcaact
     3961 tctattgtag taataaatca aaaaacatta gaagaaaaat taaaatcagg caatataact
     4021 ttagacagtt ttattaataa aatgcttgat actaaagact ctgccaatgc agcaagttta
     4081 aaaaaatggc aagattttaa aaacagtgga atagatttag ataataatct gtattttttt
     4141 gtagtacaaa aaggttccat ttcaaaaggg ctaacaactg cagtaaacat aatggcagga
     4201 ttaaaagacc aggcaaaatt tgaaaaatat attaaagaac aaaaagagtt caatgccaaa
     4261 aatttgattc aagaaaataa tttttcttac ttaagttcta ttgatactga tttatcctta
     4321 tcgtggaata aagaaattgc aattgctact ttttataaaa gagatattaa aggcagttat
     4381 gattctgtag gaaattacat agaaccggat aaatcggaaa ccacaaaaga taaaaatgaa
     4441 gaagtaaaaa gatattttaa tttaaaagaa aacgaatctg ttgcatctgt tgcttatttt
     4501 aatgatatgt ttaaaacaaa agcagatggc tatatttttg gtagcactaa tagcatgtta
     4561 ggttttttag catctacacc actaaatatt cctaaggtag aggagttact aaaagataat
     4621 tatactgcct ctacttttaa ttttgaaaat ggtaagattg aggtaaacaa tacaacctat
     4681 accaatccta tgctaagcag tattcttaaa aaatattcag gttcaagcgt aaatacccat
     4741 cttgcagagt tttttcctac acaaaactta aatggtacca ttttaatttc ctttaaacca
     4801 gaaatgtttg atggtttgtt aaaagagtta gaaataaaag gaatgttaga agcattttta
     4861 gctcagcaag gtttaaccag tgccgattta tttaaattat taagtggcga aataaacatt
     4921 gccatgtctg attttgttat agaaaataaa gaagtaacac aaaaactata tgatggctca
     4981 acctatacct ataacaaaac agtaccaaat tttaaactaa ttgctacagc accaattggc
     5041 gataaagcag catttaccaa aataatggac aaagccgtag aaaataattt attggctaaa
     5101 actagcacag gctacacttc tggcgaaatg cttgcctcta gtggtatgta ttttatggca
     5161 gatgaaaaac aaattgtaat tgccagcagc caggattttt atactactta tctaaacaat
     5221 aaaacaaaat caaatcttaa ttcaaatatt atagcagatt ttaaaggaaa aagtatggca
     5281 gcctttattg atattaacag tataattaaa ggctttactc caactgcaaa agatacaacc
     5341 attagcaata aagttttagc gatggttaat acaaccttta aaaattttat agctaccaca
     5401 gaaaatttta aagacaacag ttttaaagga aaagtaactt taaatatggt aaatgaaaaa
     5461 caaaacagtt tggtaagtat tattaattta atagatgaag caagtaaagc tattaaacaa
     5521 acaaccaatt caactacaaa ctcaatagac gaaaacactt tttaatattt taattttttt
     5581 cttctactaa agccactgat aatacggtgg ctttttaatt tactaaattc aacaaccaac
     5641 tatcattatg tatttaagct aaaatgtata aatacctaca tttgctttct aaaataaaac
     5701 aatatgaaaa aaattgcctt atttgctttt gccttataca ctgtttgtag tgtaagtgca
     5761 caaattaaaa tgcctgctcc aagtccatct caaaatattt ctcaagattt tggtttaggt
     5821 aaaattgaat taagctattc tagaccatct ataaaaggaa gaaaaatttt taaagaagga
     5881 agtgaattag tgccattaaa taatgtttgg cgtacaggtg caaatggtgc tacaacttta
     5941 aaatttaccg atgaagtggt tataaatggt gttacaatag cacctggtaa atatggttta
     6001 ttatccattc cgggtagtac aaactggaca ttaattatta caaaagatac caccgttacg
     6061 tcacctgcag catataagca agaaaatgac attgtaagaa ttgttgcaac taccactaaa
     6121 atgaagcaaa aagtagaaac ttttaccatg caatttgcca atatacaacc cgaaagttgt
     6181 gaattacatt taatgtggga taaaactgca ataagcttac ccattacaac taaaataata
     6241 gatagagtta gaacacaaac agaaaatgca ttagctgcag aaaaagtaag tggcaataca
     6301 tattatgctg ccgcaaattt ttattacgag tacgacaaaa actacaataa agcattaacc
     6361 tgtgttacaa aagcaataga aattaatcct aagttttttt ggatgtattt attaaaagca
     6421 aaaattgaaa aagcattagg agataaagta gctgccaaac aaagtgcaga aaaatgtaaa
     6481 gaaattgccg gccagcaaaa caatacagat tatgtaagaa tgagtgatga gttaattaag
     6541 agcttataat tagtagtaat ataaaattaa aaaaaggcga cacattggtc gccttttttt
     6601 aattttatcc aataaaaatc atcaggaaaa gtatattcat tatataaata aattcgtagc
     6661 tttgagagtt ggtggcaata cttttgcacc ctaaactaaa ctttaaatac tctttttatg
     6721 gacattatta acttgattga ccgcaaagaa attttatttt ttgccaaccg cacaagtggc
     6781 acattttgtt ttgctgctaa cgcacaagca aacccaaagg taatacaaaa agagccactt
     6841 cttccaacac tttaaatgat ttcgcagata tggagaaaag ctataaaggc aatttttaca
     6901 agaatcttaa tcagtcgttt ttaaaaccga gatacctatt gttcattgaa gatgaaatta
     6961 atcctacact gaatagatac atcattgaga actatgaaaa aatctcaaaa gcagttgaaa
     7021 gcaaagaaat ggagttcatt tattttcctt tcatttcaaa ggcgaccaat gagtttattt
     7081 tttccctcac tgactttttg aagtataagc atcctatact ttattcattc aatgctaagg
     7141 aaataaaaaa tataattgca tttctgattt cttcaacaga accacttgaa ttttatagca
     7201 tagtattaaa agaaattggg ttgagcagga ttgagcattc tggcttaatc cggaattttg
     7261 aaggtgcacc tgaatttgat tcaaagaaaa agttcagttt tgtatatcct agtggaagca
     7321 catatgctga gtatgagatg ttttttgaaa gcttcattag tcagttactt ataccatcta
     7381 aaataccgca aacaatattt tatcaacttg cagaagaatc aaaacctaat agttatgatg
     7441 ctgactggta ttttagcaaa gaatcaaaca tagcgtcaga cgaattgaag ctgttgattg
     7501 attcatttca aaaggaaggc aagtatgctc ttttagccga tgcagtgatg tatatgcttt
     7561 catcattgaa agacaatcaa cctgaattta ttaaaaaggt aaaaccaatt attgagcata
     7621 aacaactatt ggatactgat gtaatattga gtaaaattat ggttgacaat cactacaaca
     7681 ttttccttcc tgatttcggc aacagagaaa taaaaatgca tgcacttcca aaatctcttt
     7741 atcttctttt tcttcgctat cctaacggaa tacgctttaa ggaactttat gaatataaga
     7801 aggagttact tgaaatctac aacaaagtga ctaacaaata cgagaaagaa gaaattgtaa
     7861 gagcaattga tgatttggtt gatatgacga agaattccat caatgaaaag tgttcccgta
     7921 ttagagaagc atttgtatca ataatggatg ataatacagc aaagcattac tacattactg
     7981 gtgaaagagg gaaacccaag aaaattttat taccaaacaa attaattgac attcgctatt
     8041 gatgaaagtt catctaatta aaacaccgga atatagccct gaagatttca gagatgtcag
     8101 cgaattgttg aattcgtttg atggtgcttt agaatttgtt gcttctgaat atgaatttga
     8161 caaagagcaa ttcccattcc ttcaaaagtt ttacccggat ttcaagttca agtatgaatc
     8221 tgacattaaa aaattaaatt tcaacaaaga acttggattg cctttaagtt ggcgagaatt
     8281 attttctcta tgcgattttt atagaacaac cttcaacatt aatgaaacag attttattat
     8341 tgtgctaact atcaggcgta atgctttgaa ttggtttagt cattgtgaaa acaaaaatgc
     8401 ttttgttcac acaggcgatt gggaatattt tacagatgct aaccctaaat atccagtagc
     8461 acatcaaatt gtcgagaatg tgatgcagtc actaatgaaa cttgatactg ttaatattcc
     8521 aaacatcaat gttcatacag attcaagagg gtgcatgaat gatttttgtc agaacaaagg
     8581 acaaataatc ctcaaactaa gaacagcgga tatttgtcct gactgcattg aaaaaattca
     8641 agaagaaaat attgaccata atattgtcaa tcaagtttta aaaatctttg aaggaattcg
     8701 taacgaactt ttattcaaac aaaaatttaa aaaacaggtt acaccagtcc caattacaat
     8761 taacgagagg aatcagatta ttcttccaca acttggcaat cttgaaatcc gactcaatcc
     8821 tcttttcaaa acgctctaca ttttttattt aagacacact gaaggtgtga gattgaatga
     8881 attaaatgat ttcaggtcag aacttttgtc tctctacaga agactatcag ttgctgacaa
     8941 caatcaaaca attgaactaa gaattacaga ccttgtaaat ccaattggtg gctcattcag
     9001 tcaaaaaaaa tctaaactga ataagattat taccgacctg ttaggagaac ccttggcaaa
     9061 attttatcgc atagaaggag agccgggtga accctttaaa atcaacctcc ccaacaaact
     9121 tattgacatc agatattgac tttttgcaac cttgcaaaac ccaatgcaag gttgcagaga
     9181 ttaggtaagc cctgacagcc attctacttt tgcttcatca aaccgataat cggattggct
     9241 aagaaaaaga gaacgagcaa aatgatttac caagaaacag aaactttaag agagttagcc
     9301 aagagttgga ataaccttga caccagcttt attgaaaagc atttatctga cgatttcgtt
     9361 tatgaatcgc aatgggtgct aacaccgatt gaaggaaaac gagagttcct caattatctg
     9421 caatcaaaat ttcaggcaat aaaatctaca atgcaaaaag aaacaatgat tgtttcagct
     9481 gagttggctt cgcatccagc tatgcaaaac aggccaatta ttgttctcac tcaaataacc
     9541 aaagaaggaa taaggcaagt ttcattgcta atcaaaattg agaacgacaa gttgaaacgc
     9601 attgatgtat gtttcatacc cgacccaagc gaagcagaat taaccgggga atttccaaag
     9661 taattattaa atcaataaac atatgacaac agaatacaac actctattaa aaagtccaca
     9721 atggtttgag aaaagaaaaa ttattttggc aagagacaac aataagtgtc gttgttgcgg
     9781 tgcaacaacc agacttattg ttcatcacag acaatatcat gtaagcaagc ggacaggaag
     9841 ttttcaactt ccttggaagt atcagaacaa atatctgatt acgctttgcg acaagtgcca
     9901 cacaacaggg cattcaaaat ttaaagttcc aacattcaca atttaataaa caataaaaat
     9961 taaaaacaat gagtattata gaaaagctat ttggcggcaa agccaaaaca gaaaatccgg
    10021 taacaacgat taccgaacct cagtcaacag ttgcagagca acctgaaaat attccaccag
    10081 tggacttgtt tattgacaaa gaagttcccc aacaggagca acaacaagta gcagaatcga
    10141 aaagtaggat tactcttttc cttgacagta attaccactc aataggaatc aatgatggtt
    10201 atgagtatca ttcacaagaa acgcttgaaa ctggcaagaa aaaaattcgg gcagagtttc
    10261 agctaattgt tgaccaagca attcaagaaa aatcagaaag gagattgcag ttaagaatga
    10321 tgattgtcaa tgttgagaag attgctgaag aaacaaaacg aaatcttgaa ttgaccattg
    10381 aggaactcaa ctcttccatt gctctacttc aaaagcaaaa agaactctca gcagaaaatg
    10441 aaggttgggt tatgaacgct attcatccct atcatcaagg ttatgtgcaa ggtgttaata
    10501 ttcgtattga atcagagaat tttttgaatc caattaaaaa catttaaaac taaaagtcat
    10561 gttaaaatat tatagcatca tcatcggaga gaatccagaa tatacaaaga cattccaacc
    10621 ggcaagcaaa cgcaagattg ctttgtatgc aaactgttta atgattccgg ttattctttg
    10681 gtccattaat agttaccttc tagtaagtca tgttttagag ggaagttttt ttgctgcaat
    10741 cttcactgca attcttgcag ggtttatcat cttccttatt gaaagagcca ttattatgtc
    10801 aaacggaagc aaaccaatat tttggttcag aatcctctta ggttttatag tagcttctct
    10861 tggttcagtt agtatggatg aagtaatctt caaacacgat attgacaatc aggttgcata
    10921 ttacaagcaa gctgaaattg acagtgcagt tcacaaagtt gaaagggatt atcaaaatca
    10981 aatttccatg caacaatcaa ttgtaaaaca aaaggggtct gaatggaatc aatctttgaa
    11041 ggatgcaaag agtgatgcag acggaacagg tggtagtcgc caaagaggtg tgggtaaaat
    11101 ttcaactcta aaattaaata ttgcaggaaa gcaagaagta gattacaaca ctgagaataa
    11161 caaacttact tcactgattg caagtgttga cacagcaaaa gcccatgcta aagaaaaagc
    11221 agaaaccgat tttaacggaa atgctttact cataagaata cg
//
LOCUS       DAOQHP010000023        10873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3017_length_10873_cov_3.915511, whole genome shotgun sequence.
ACCESSION   DAOQHP010000023 DAOQHP010000000
VERSION     DAOQHP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_10873_cov_3.915511"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLY81_01620"
     CDS             complement(<1..793)
                     /locus_tag="PLY81_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNF28960.1"
                     /translation="MIEVGKFNTLVINRKVDFGFYLDNGAEGILLPKRFAPANAKIGD
                     TVNAFIYHDSDNRLIATTQQPNGVVGDIVLMKCVSTMSAGAFLDWGLMKDLFVAKSQQ
                     KVGMKVNGWYLVKIYLDVQTGRVAASEKIEKYLDNSDLTVAEKEEVDIVIYRKTDIGY
                     SCIINNRHYGLIHNNQVFQNLEVGDLLKGFVQTIREDNKIDIVLGKVGYKKTEDEVEK
                     IIRLLKESNGYLPYHDKSEPDEIYNYFGMSKKTFKMAVGALYKQRK"
     gene            915..1478
                     /locus_tag="PLY81_01625"
     CDS             915..1478
                     /locus_tag="PLY81_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNF28961.1"
                     /translation="MNQQYILNTIQLFEYYKSLGDKTFNQLQEKDLFWQYNNNSNSIA
                     IIVKHLCGNMLSRWTDFLTTDGEKEWRKRDEEFENDLKNKEELLHKWKEGWDCLFKAL
                     NSLTEKDLNNTVYIRNQGHTVTDAINRQLAHYASHIGQIMYIGKMIKDADWQSLSIPK
                     GNSQQFNQEKFAKEKHDEHFTNEALGK"
     gene            complement(1703..3307)
                     /gene="pckA"
                     /locus_tag="PLY81_01630"
     CDS             complement(1703..3307)
                     /gene="pckA"
                     /locus_tag="PLY81_01630"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNF28962.1"
                     /translation="MSIPSFSIPTEKLLTLGISDVTKVHYQLSPSELVQQALDRKQGV
                     LNSTGALCINTGEFTGRSPLDKFVVKDNITANSVHWNNFNIPIEDKYYQQLKRKVLNY
                     LSQQGEIWVRDAYACASPEHRINIRVINENPWSNLFAYNMFLRPTEAELSNYPVDWHI
                     IQAPGFRADASIDGTRQHNFAIVSFTEKTILIGGTGYTGEMKKGIFTILNFVLPHDKN
                     VLSMHCSANMGKDGDVAIFFGLSGTGKTTLSADPNRKLIGDDEHGWTNDSVFNFEGGC
                     YAKVIDLSAEKEPEIFNAIRPGAILENTTFLDGTNEIDFASKKITENTRVSYPLNFIS
                     NALEPSIGGLPKNIFFLTCDAYGVLPPISKLTPGQAMYQFISGYTAKVAGTEAGVTEP
                     KSTFSACFGAPFLPLHPGFYAEMLGKKMKENKVNVWLVNTGWSGGGYGVGQRMKLSYT
                     RAMITAALNGELNNVVFEKHPIFGVEMPTTCPNVPENILNPKNTWNDKEAYDAAAKNL
                     AAQFIKNFEKYAAGVTEEILASAPKA"
     gene            complement(3507..4118)
                     /locus_tag="PLY81_01635"
     CDS             complement(3507..4118)
                     /locus_tag="PLY81_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNF28963.1"
                     /translation="MKKSDFNLQVQNDNIESKIVASLERIAQAFRVLLWQETKEFSLS
                     PIQVQILIFLLNHSKDKCKVSYLAAEFNLTKATISDTIKTLEQKKLIEKEYEVEDSRS
                     YIIHLTQKGKEIAQKTSFFTNEIKSPISKLSSENKEHLYCSLLDIIKYLNTQKIVTIQ
                     RSCINCTHYQTNKNGLKHFCKLLNSNLSTSQVRIDCPLHQEKL"
     gene            complement(4258..5502)
                     /gene="clpX"
                     /locus_tag="PLY81_01640"
     CDS             complement(4258..5502)
                     /gene="clpX"
                     /locus_tag="PLY81_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNF28964.1"
                     /translation="MAKQSHLHCSFCGRSRDEVKILIAGQEGHICESCVEHALEIIEQ
                     EIHTSDKSKSNTNLNTKLTIRKPQEIKKFLDEYVIGQDEAKKILAVAVYNHYKRISHK
                     QLPDEVEIEKSNIIMVGETGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVES
                     MLTRLLQNCNYDVAAAEKGIVYVDELDKIARKGDNPSITRDVSGEGVQQGLLKMLEGT
                     EVLVPPQGGRKHPEQKMIKIDTKNILFICGGAFDGVDKVIARRINTNAIGFNVNKEEQ
                     ELQRNNLLRFVNAQDLKKFGLIPELLGRLPVVTYLNPLDAATLRNILTEPKNSIIKQF
                     TKLFELEGIKLKIDDEVLDFMVSKALEYKLGARGLRSICESIFTDAMFEMPGTNKTEL
                     HITANYAENMFNKSKLNLLKVA"
     gene            complement(5629..6267)
                     /locus_tag="PLY81_01645"
     CDS             complement(5629..6267)
                     /locus_tag="PLY81_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNF28965.1"
                     /translation="MLSSNKIINPFKLEDEEEDNKKDEKQEQLSPGMMKKMEKLFFEK
                     RAVYLWGVVEDKSIREVVSKILLLEADKPGKEIKLYINSPGGVVTSGMVLFDTMTMIS
                     SPVSTICMGLAASMGSILLCAGTKGKRYIYPHGEVMIHQPSISGYYQATSADIEIQAI
                     QIEKTKLASAKILAKNCGKTVEQVLADFDRDYWMDAQEAVNYGIVDEILHKL"
     gene            complement(6300..6989)
                     /locus_tag="PLY81_01650"
     CDS             complement(6300..6989)
                     /locus_tag="PLY81_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNF28966.1"
                     /translation="MNIEQEFKKFAIKNQGISSGVLHNYQTTFSPTNLTPYIIEERPL
                     NIAQMDVFSRLMMDRIIFLGESINDYVANIITAQLLFLESADRTRDIQMYINSPGGSV
                     YAGLGVYDTMQFISPDVSTICTGIAASMGAVLLCAGVQGKRTALKHSRIMLHQPSGAI
                     GGQATDIGITAKEIKILKQELYEVIAHHSGKDIETVSKDCDRDFWMKAIDAKAYGLVD
                     EVLFTNPKKEK"
     gene            7188..8333
                     /locus_tag="PLY81_01655"
     CDS             7188..8333
                     /locus_tag="PLY81_01655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF28967.1"
                     /translation="MSSTVIKKVIIIGPAHPLRGGLASFNERLAKQFNQENIETTIYT
                     FSLQYPNFLFPGTTQYSSEPAPKNLDINVCINSINPFNWLKIGWQLKKAKADIIVVRY
                     WLPFMGACLGTILRIVKKNNKTKIVCIADNILPHEKRIGDKQLTSYFLKPIDAFITMS
                     EKVMKDLRMFTNKNAVKVEHPLYDNFGEAIDKKTARQILGLNTNDKIILFFGFIRKYK
                     GLDLLFEAMKILQVKYGHLNIKLLIAGEFYENRKEYDEQINSLNIASNLILKTDFIAD
                     SEVGYYLSAADFVIQPYKNATQSGVTPLAYHFNKPMLVTNVGGLPALVPDKKVGLIAE
                     PNSNDIAEKIVALYEFGEEYFIPYLQEEKKKYSWQNLTNTIIKLAQK"
     gene            complement(8448..9926)
                     /locus_tag="PLY81_01660"
     CDS             complement(8448..9926)
                     /locus_tag="PLY81_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28968.1"
                     /translation="MKKNNIVVFLFLILLAATGCKKSLLDVRNENDPDFKSVLSNGSD
                     VETLTSSLFNTIYDGEHSYSGCSMMLATAADHVSCSWGNAGMRDMSYEPRNNAWNNSS
                     AYSNKGFTKYTFDKMYATINTASQIMKALSNGVVVDNPAKIKAFAKYAQGVAYGNLAL
                     IYDRAFITDENVTVPGELASAVDYKQVATAAIGYLNEAITQSANTFTIPASWLGTAAD
                     LTNADFKALCNTMAARILAYTPRNKAELATVNWAQVKTYADAGITFDFNVMNDNYVKW
                     YDEAGDYLVYNGWGISDTYTINMMDPTLPQHWDDSPSFPYPAASVSPIDQRLNTDFEY
                     VAAQWLNPARGYYHFSPYRNKRYDAMYVNATGAKPEVMKAENDMLRAEARAYTADVAG
                     AAAIINLSTRKTRGNMADVAAVLADVVKAIHHERFVEMYTTGIGLQFFEMRKLDLLQK
                     GTPLHLPLPAGTLETLNAPLPFYTFGTVAKADGTGTSNGGWR"
     gene            complement(9946..>10873)
                     /locus_tag="PLY81_01665"
     CDS             complement(9946..>10873)
                     /locus_tag="PLY81_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28969.1"
                     /translation="IPFLNDGFPSQWQNAGNVKSNTIEITLGANWKNTPNLKWNSSLV
                     FSRVRQKIGTLPIPPYMFTNQNMGDQYIFRVTSNEAYGAMYGYRFVRTLAEMANQLPT
                     GKTIADYEVNSEGYVIDKGTQGTVNERPIKYKENGADWYGKIGDGNAKFNIGITNTIT
                     WKDFSFYFLLDWKQGGDIYNGKDQRLAFNNMSIRQDMSGVAANEKKAYDYWFSGAYDG
                     NNANQYWVEDGSYLKIREISIGYNLNNKILKKIFNGAIKGVNAKIVGRNLFTFTKYSG
                     YDPEVGTIRQPYDGIYMNPNFRNYAFSLSFNF"
BASE COUNT         3562 a         1845 c         1508 g         3958 t
ORIGIN      
        1 tttttcgctg tttatacaat gcaccaactg ccattttaaa agttttttta ctcattccaa
       61 aataattata gatctcatct ggttcacttt tatcgtgata aggtaaataa ccattactct
      121 cttttagtag cctaattatt ttttctactt cgtcttctgt ttttttatag cctactttac
      181 caagtacaat atctatttta ttatcttctc ttattgtttg tacaaaacct ttcagcaaat
      241 cgccaacttc taagttttga aaaacttgat tgttatgaat tagtccataa tgccgattgt
      301 taataataca actatagcca atatctgttt ttctataaat tacaatatct acttcctctt
      361 tttcggcaac tgttaaatct gaattatcta aatatttttc aattttttca gaagcagcaa
      421 ctctacctgt ttgaacatct aaataaattt ttactaaata ccagccgtta actttcatgc
      481 ctactttttg ttgcgatttt gcaacaaaca agtctttcat taaaccccaa tctaaaaaag
      541 cacctgccga catagtgctt acacatttca ttaaaacaat atctcctaca acaccatttg
      601 gctgttgggt tgtagcaatt aatctattat cgctatcatg ataaataaag gcatttaccg
      661 tgtctccaat tttagcattt gctggtgcaa acctttttgg taaaagaatg ccctctgctc
      721 cattatctaa ataaaagcca aaatctacct ttctattaat tactaatgta ttaaacttac
      781 ctacttcaat catactaaat aaaaattatt cgaagttaat aataacttta cttaatacag
      841 ccatttatca tttttaataa aatattttac aactttattt attttagagt attgataaat
      901 caaaaaataa ttttatgaat caacaatata ttttaaacac tattcaactt tttgagtatt
      961 ataaatcttt aggagataaa acttttaatc agttacaaga aaaagatttg ttttggcagt
     1021 ataataataa tagtaacagt attgccataa ttgttaaaca tttatgtggg aatatgcttt
     1081 ctagatggac agatttttta acaacagatg gcgaaaaaga atggagaaaa agagatgaag
     1141 aatttgaaaa tgatttaaaa aataaagaag agttattaca taaatggaag gaaggatggg
     1201 attgtttatt taaggcttta aacagtttaa ctgaaaaaga tttgaataat acagtttaca
     1261 taagaaatca aggtcatact gttactgatg caattaatag gcaattggct cattatgcat
     1321 cgcatattgg gcaaataatg tacattggta aaatgattaa agatgctgat tggcaatctt
     1381 tatccattcc taaagggaat tctcaacaat ttaatcaaga aaaatttgct aaagaaaagc
     1441 atgatgagca ttttactaat gaagcattag gaaagtagtt ctttacaaac tcaattatgc
     1501 tattttaatt ttctactttt atttcaacta cttgctttgt atatgtaatt gtaggaggtg
     1561 atttatatac cttaaataat ttgcatatta tgtatatacg aatttatgaa cctgtaaaaa
     1621 ctaaaaaatt agaggcaata gtttttaaaa aaatataggc tgccttacaa cagacagcct
     1681 atatacaatt attaaaaaaa tattaagcct taggagcaga tgctaaaatt tcttctgtaa
     1741 caccagcagc atatttttcg aagtttttta taaattgtgc agctaaattt ttagcagcag
     1801 catcgtatgc ttctttatcg ttccatgtat ttttaggatt taagatattt tctggaacat
     1861 taggacaggt tgttggcatt tctacaccaa aaattgggtg tttttcaaat actacattat
     1921 ttaactctcc atttaaagca gctgtaatca tggctcttgt atagcttaat ttcattcttt
     1981 gacctacgcc ataaccacca ccactccagc cagtattaac cagccaaaca ttaactttat
     2041 tttctttcat ttttttaccc aacatttctg cataaaaacc tggatgtaat ggtaaaaatg
     2101 gtgctccaaa acatgcactg aatgtacttt taggctcagt aacacctgct tctgtacctg
     2161 caacttttgc agtataaccg ctaataaatt ggtacattgc ttgtcctggt gttagtttac
     2221 taattggagg taaaacacca taagcatcgc aggttaaaaa gaaaatattt ttaggtagcc
     2281 ctccaatact tggctctaaa gcattggata taaagtttaa cggatagctt acccttgtat
     2341 tttctgtaat ttttttgcta gcaaaatcta tttcatttgt accatctaag aaagtggtat
     2401 tttctaaaat tgcacctggg cgaatggcat taaaaatttc cggttctttt tctgcactta
     2461 aatcaataac ttttgcataa catccgcctt caaaattaaa tacactgtca ttggtccagc
     2521 catgctcatc atctcctatt aatttacgat ttggatcagc acttaaagtt gtttttcctg
     2581 ttccacttaa tccaaaaaat atggccacat caccatcttt tcccatatta gcactgcaat
     2641 gcatgcttaa tacgttttta tcgtgtggta aaacaaagtt taaaatggta aaaattcctt
     2701 ttttcatttc accagtataa cctgtaccac caattaaaat tgttttttct gtaaatgaaa
     2761 ctattgcaaa attatgttgg cgtgtaccat ctatactagc atctgctcta aagccaggag
     2821 cttgaataat atgccaatct acaggataat tggataattc tgcctccgtt gggcgaagaa
     2881 acatattgta agcaaataaa ttactccaag ggttttcatt aataacccta atatttattc
     2941 tatgttctgg gcttgcacaa gcataagcat ctcttaccca aatttctcct tgctggctta
     3001 agtaatttaa tacttttctt ttcaactgct gatagtattt atcttcaata ggaatattaa
     3061 aattattcca atgtacagag ttagcagtaa tattgtcttt aactacaaat ttatcaagtg
     3121 ggctacgtcc tgtaaattca ccagtattaa tacataaagc tccagtacta tttaaaacac
     3181 cttgctttct atctagggct tgttgtacta attctgatgg ggatagctga taatgaactt
     3241 tagttacatc tgaaattcct aacgttaata atttttctgt tggaattgaa aaggatggaa
     3301 ttgacatatt taaacaaaca tttgttagtt tgcaaaaata aggattatca atatccaaaa
     3361 tattttttgt agggcattat atgtttacat tcttagttaa aaataaattt taataatttt
     3421 aatgccatta ttaaatactg aaaatcaaat aattactatt tattttctat tctattttgt
     3481 gcatccagtt ttataggtgt ttacgttcat aatttttctt ggtgcaaggg acagtctatt
     3541 cttacttgtg aggtagataa gttagagttt aataatttac aaaaatgttt taggccgttt
     3601 ttattagttt gataatgggt acagttgata caacttcttt gaatggttac aattttttgg
     3661 gtattcaaat acttaattat atctaataag ctacaatata aatgttcttt attttctgaa
     3721 gagagttttg aaattggcga ctttatttcg ttggtaaaaa aagaagtttt ttgagctatt
     3781 tctttacctt tttgtgttaa atgtattata tagcttctgc tatcttctac ttcatattct
     3841 ttttcaatta attttttttg ctcaagcgtt ttaatggtat cgcttatcgt agcttttgta
     3901 aggttaaatt cggcagctaa gtaacttact ttacatttat cttttgaatg atttaataaa
     3961 aaaattaaaa tttgtacctg tattggactt aaagaaaatt ctttagtttc ttgccaaagt
     4021 aaaacacgaa atgcttgtgc aattctttct aaagaagcaa caattttgct ctcaatatta
     4081 tcattttgaa cttgtaagtt aaaatcagat ttcttcatta tttattatta cgctaaaata
     4141 gtttctgcaa aaaatacagt ttaaaagaaa aagtcccata aaggtagtta tcctaactat
     4201 atgggaaaaa taatttagct taaaataaca attaaaattt agtgaaaaaa ttgctactta
     4261 tgcaacttta agaagattga gtttgctttt attaaacatg ttctctgcat aattagcagt
     4321 aatatgaagt tctgttttat tggtaccagg catttcaaac attgcatcgg taaatatgct
     4381 ttcgcaaata cttcttaagc ctctagcccc taatttgtat tctaatgctt tgcttaccat
     4441 aaaatctagt acctcatcat ctatttttaa cttaattccc tccaattcaa ataattttgt
     4501 aaactgctta ataatagagt ttttgggttc agttaaaata tttcgtagag tggcagcatc
     4561 taaagggttt aaataagtta caacaggtaa tctacctaat aattcaggta ttaggccaaa
     4621 cttttttaaa tcttgagcgt ttacaaatcg tagtaaatta tttctttgta attcttgttc
     4681 ttctttgttt acattaaatc caatagcatt ggtatttata cggcgagcta taaccttatc
     4741 cacaccatca aatgcacctc cacaaataaa taaaatattt ttagtatcta ttttaatcat
     4801 tttttgttca ggatgctttc taccaccttg tggtggaact aacacttcgg ttccttctaa
     4861 catttttaat aacccttgtt gtacaccttc tccactcaca tctcttgtaa tagaagggtt
     4921 atctccttta cgtgctattt tatctaattc atctacatat acaatacctt tttcggcagc
     4981 agcaacatcg taattacaat tttgcaataa ccgtgttagc atactttcaa catcttctcc
     5041 tacataacca gcctcagtaa aaacagtagc atctacaatg gcaaatggta catttagcat
     5101 acgggcaata ctttttgcaa gtaatgtttt gcctgtacca gtttcgccaa ccattattat
     5161 attactcttt tcaatttcaa cctcatcagg taattgttta tgagaaattc ttttataatg
     5221 attataaaca gcaactgcca gaattttttt tgcctcgtct tgtcctatta catattcgtc
     5281 taaaaacttt ttaatttctt gaggtttacg tatggtaagt tttgtattaa gattggtatt
     5341 acttttagat ttatctgaag tatgaatttc ttgttcaata atttctaaag catgctctac
     5401 acaactttcg caaatatgcc cttcttgccc agcaattaaa attttcacct cgtctctgct
     5461 acgaccacaa aatgagcaat gtaaatgact ctgctttgcc attattaatt aaatgtagat
     5521 gttattaata gattaattag tacaagtata acgtaaatac ttataaaatt aatgtacaaa
     5581 tgtaaattaa attataccaa tgagtataaa aaaagtatta aaatagtttt aaagcttatg
     5641 taaaatttca tctacaatgc cgtaattaac tgcttcttgt gcatccatcc aataatctct
     5701 atcaaagtct gcaagaactt gctcaacggt tttgccacag ttttttgcta atattttagc
     5761 actagctaat ttggtttttt ctatttgaat tgcctgaatt tctatatcgg cactagttgc
     5821 ttgataatag ccacttatac ttggttggtg tatcattacc tcaccatgtg gatatatata
     5881 ccttttgcct ttagtacctg cacatagtaa aatggaaccc atactagcag caagacccat
     5941 acatattgta ctaacagggc tgcttatcat tgtcattgta tcaaataaaa ccatgccact
     6001 ggttacaacc cctcctgggc tattaatata aagcttaatt tctttgccag gtttatctgc
     6061 ctctaacaat aaaattttac ttactacttc tcttatactt ttatcctcta ccacgcccca
     6121 taaatacaca gctctttttt cgaaaaaaag tttctccatt ttcttcatca ttcctgggct
     6181 taactgctct tgtttttcat cttttttatt atcttcttct tcgtcttcta acttaaaagg
     6241 gtttattatt ttattgctac ttaacatata ttatttttga atttacggaa tgaaaaaagt
     6301 tatttttctt ttttaggatt ggtgaaaagt acttcatcaa ccaaaccata agctttggca
     6361 tctatggctt tcatccaaaa gtctctatca caatctttag aaacagtttc aatatctttt
     6421 cctgaatgat gagcaattac ttcatacaat tcttgtttta aaatttttat ttcttttgct
     6481 gttataccaa tatctgttgc ttgcccacca atggctccac ttggttgatg cagcataata
     6541 cgactatgtt ttaaagcggt tctcttacct tgtacacctg cacacagtaa tactgcaccc
     6601 atacttgctg caatacctgt acaaattgta cttacatccg ggcttataaa ctgcattgta
     6661 tcataaactc ccaaacctgc ataaacacta ccacctgggc tatttatata catttgaata
     6721 tctcttgttc tatctgcact ttctaaaaat aaaagttgtg cagttataat atttgctaca
     6781 tagtcgttaa tactttctcc taaaaaaata attctatcca tcattagcct actaaacaca
     6841 tccatttgag caatatttaa tgggcgttct tcaataatat atggtgttaa attagttgga
     6901 gaaaaagttg tttgataatt atgcaaaacc ccactgctta tgccctggtt ttttatggca
     6961 aattttttaa attcctgttc tatattcatg ctattaattt tgcgttgaag ataagtgttt
     7021 tgaaattttg ataaaatttt tatccaaata atatgcaaaa aagcaaaaac cgacaaaagg
     7081 gcatagtaat aatataattt aatacacatt gctgttttta gtaaattatg gtataacaac
     7141 tatgcattat tatctttgat ttatttgaaa aaaatattga caataaagtg tcttctacag
     7201 ttataaaaaa agtaattatt attggccctg ctcacccttt aagaggtggc ttagcttctt
     7261 ttaacgaaag gcttgccaaa cagtttaatc aagaaaatat agaaacaacc atttatacct
     7321 tttctttaca atatcctaat tttttatttc caggcacaac acaatattct tctgaacctg
     7381 ctcccaaaaa cttagatatc aatgtttgta tcaactctat taaccccttt aactggctta
     7441 aaataggatg gcaattaaaa aaagccaaag cagatattat tgttgtacgc tattggctgc
     7501 cttttatggg agcttgctta ggaaccattt taagaattgt aaaaaaaaat aataaaacaa
     7561 aaattgtttg tatagctgat aatattttac cccatgaaaa aagaattggc gataaacagt
     7621 taaccagtta ttttttaaag ccaatagatg cttttattac tatgagtgaa aaagtaatga
     7681 aagatttaag aatgtttacc aataaaaatg ctgttaaagt tgagcatcct ttatacgata
     7741 attttggaga agcaatagat aaaaaaacag ctaggcaaat cttaggctta aataccaatg
     7801 ataaaataat tttatttttt ggatttatta gaaaatataa agggttagat ttattattcg
     7861 aagccatgaa aattttgcaa gtaaagtatg gccatttaaa tattaaacta cttattgcag
     7921 gtgagtttta tgaaaataga aaagaatatg atgaacaaat aaattcatta aacattgctt
     7981 caaaccttat tcttaaaaca gactttattg cagatagcga agttggctat tatttaagtg
     8041 cagcagattt tgtaatacaa ccttataaaa atgcaactca aagtggtgta accccattgg
     8101 cttatcattt taataaaccc atgttggtta ctaatgttgg agggttacca gccttggtac
     8161 ctgacaaaaa agttggttta attgccgaac caaattcaaa tgatatagct gaaaaaattg
     8221 tagctcttta cgaatttggt gaagaatatt ttatacccta cttacaggaa gaaaagaaga
     8281 aatatagttg gcaaaacctt accaatacta taattaaatt agcacaaaaa taaaatgtgt
     8341 tttattaata cttttataat tgtagttacc aactttttga ctatttatta tttaaaaagg
     8401 agttgtattt tacaactcct ttttaaattt tattttacaa cactatctta tctccatcca
     8461 ccatttgatg tacctgtacc atctgctttt gcaacagtac caaaagtata aaatggcaat
     8521 ggtgcattta atgtttctaa agtacctgct ggtaaaggca agtgtaaagg cgtacctttt
     8581 tgtaataagt ctaattttct catttcaaaa aattgaagac caataccagt tgtgtacatt
     8641 tcaacaaatc tttcgtgatg tattgcttta actacatctg caagtacagc agcaacatct
     8701 gccatattac ctcttgtttt acgtgtactt aaattaataa ttgctgcagc accagctaca
     8761 tcagcagtat aagctcttgc ttctgcacgt aacatgtcat tttcagcttt cattacctct
     8821 ggttttgcac cagttgcgtt tacatacata gcatcgtatc ttttatttct ataaggagaa
     8881 aaatggtaat aacctcttgc agggttaagc cattgtgctg ctacatattc aaaatctgta
     8941 tttaatcttt gatctattgg agaaacagaa gctgcagggt aagggaatga aggagagtca
     9001 tcccaatgtt gtggtaatgt tgggtccatc atattaattg tatatgtatc agaaattccc
     9061 caaccattgt aaacaagata atctcctgcc tcatcgtacc atttaacata gttatcgttc
     9121 attacgttaa aatcaaaagt aataccagca tctgcatagg tttttacttg agcccaattt
     9181 actgtagcca actctgcttt attacgaggt gtataagcaa gaattctggc agccatggta
     9241 ttgcataatg ctttaaaatc tgcattggtt aaatctgctg cagttccaag ccagcttgca
     9301 ggaattgtaa aagtattggc actttgggta atagcttcgt ttaagtaacc aattgcggca
     9361 gtagcaactt gcttataatc tacagcactt gcaagttcgc caggaactgt aacattctca
     9421 tctgtaataa atgccctatc ataaattaat gcaaggttac cataagctac gccttgtgca
     9481 tattttgcaa aggctttaat ttttgcaggg ttatctacca caacgccatt acttaatgct
     9541 ttcataattt gagatgcagt attaattgtt gcatacattt tatcgaaagt gtatttagta
     9601 aagcctttat tgctatatgc agaggagttg ttccatgcat tgttacgtgg ctcataagac
     9661 atgtctctca tacctgcatt accccaagag caagatacat gatcggcagc agtagcaagc
     9721 atcatagagc aaccactata actgtgttct ccatcataaa ttgtattaaa taaactagaa
     9781 gttaaggttt ctacatcact tccattggaa agcacacttt taaaatcagg atcgttttca
     9841 tttctaacgt ctagcaggct ttttttacaa ccagttgctg ctaacagaat aagaaataag
     9901 aaaactacaa tattattttt tttcatattt tgtaattttt aaaaattaga aattgaaaga
     9961 taacgaaaat gcatagttcc taaaattagg attcatataa ataccatcat aaggttgtct
    10021 aatagtacct acttctgggt cgtagccaga gtattttgta aatgtaaata ggtttctacc
    10081 aacaattttt gcatttacac ctttaatagc accattgaat atttttttca atattttatt
    10141 attcaaatta taccctatag atatttcacg aatttttaag taacttccat cttccaccca
    10201 atattgattt gcattgttac catcataagc tccagagaac caataatcgt aagctttttt
    10261 ctcattagct gcaacaccac tcatatcttg acggatagac atattattaa atgctaagcg
    10321 ttggtcttta ccattataaa tatcgccgcc ttgtttccaa tctaataaga aataaaatga
    10381 aaaatctttc caagttatgg tgttagtaat accaatatta aatttagcat ttccatctcc
    10441 aatttttccg taccaatcag caccgttttc tttatattta atagggcgtt catttactgt
    10501 accttgggta cctttatcta taacatatcc ttcgctatta acttcataat cggctatagt
    10561 tttaccagtt ggaagttgat ttgccatttc tgctaaagtg cgtacaaatc tgtaaccata
    10621 cattgcccca taagcttcat tagatgttac acggaaaata tattggtcgc ccatattttg
    10681 gttggtaaac atatatggag gaattggtaa agtgccaatt ttttgacgaa ctcttgaaaa
    10741 tactaaggaa gaattccatt taagatttgg agtgtttttc cagtttgcac ctaaagtaat
    10801 ttcaatggta tttgatttta cattacctgc attttgccat tggcttggaa aaccatcatt
    10861 taaaaaagga att
//
LOCUS       DAOQHP010000024        10851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3028_length_10851_cov_3.851334, whole genome shotgun sequence.
ACCESSION   DAOQHP010000024 DAOQHP010000000
VERSION     DAOQHP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_10851_cov_3.851334"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..3290
                     /gene="uvrA"
                     /locus_tag="PLY81_01670"
     CDS             432..3290
                     /gene="uvrA"
                     /locus_tag="PLY81_01670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF28970.1"
                     /translation="MANDNFDNSKIIVQGARENNLKNIDIAIPKNKLVVFTGVSGSGK
                     SSLAFDTIYNEGQRRYMESFGAYARQFLGNMERPDVDKISGLSPVISIEQKTTNKNPR
                     STVGTVTELYDFLRLLFARIGEAYSYNTGKKMVKLSEEEIIQTIFEKFKEEKILILAP
                     LVRGRKGHYRELFEETRKKGFLKVRIDGEVKDLTPKLQVDRYKVHNIEIVVDRLEVTM
                     NMKLRLSQSIQQSLKIGKDLIFVASANQISNNKDAVFSKQLMCVDTGISYEEPSPNTF
                     SFNSPYGACPTCKGLGVEYNINIDAVLPNKSKTIKEGGIVPLGEERDASVFKQVVDFA
                     KKNKISLDTPLEKLSDQQLNLILFGDKNITQDLVINMNDENIPNEYTGSYEGIIPMLK
                     RWFTSTNSTDFLQGWVEKFMTQGICSSCNGKKLRKESLWIKINNKNISDISEMNLDKL
                     MLWLNTIEHNLNSKQNIIAKDILKEIRERLQFLLDVGLSYLSLNRSSKSLSGGEAQRI
                     RLATQIGSKLQGITYILDEPSIGLHQRDNHKLIDSLKNLRDIGNSILVVEHDKDIMLA
                     SDYLVDIGPKAGKHGGQIVAFGTPKEILKSNSDTAQYLSGKKAISIPNVRRKGNGKFI
                     ELIGASGNNLKTITTKLPLGNLIVITGVSGSGKSTLINETLYPILSKHCYKSNIPIKE
                     FKSINGLENIDKVIEIDQSPIGRTPRSNPATYCGFFTEIRTLFASVPEAKIRGYTAGR
                     FSFNVKTGRCEVCEGGGMKVIEMNFLPDVYVHCEKCNGKRYNRETLEIRYKGKSISDV
                     LNMTVDEACEFFNAVPWLYRKIKVLQDVGLGYLTLGQSAVTLSGGEAQRVKLSTELGK
                     KDTGKTFYILDEPTTGLHFQDIQLLMEVVNKLVDRGNTVLIIEHNLDVIKLADYIIDL
                     GPEGGDGGGEILFEGTPEEMILNKESYTAKFLKQELSN"
     gene            3384..8123
                     /locus_tag="PLY81_01675"
     CDS             3384..8123
                     /locus_tag="PLY81_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF28971.1"
                     /translation="MKLVLLSTFLIFHLFVFSQTNSEIDFKAHFEKGTNLLLAEKYDS
                     ALYYFNLSEKIAEKQNKYSDDKYSSLNINKGICYMYLQEPDNAHYYMYKGLLNARKFK
                     HIIPESNAILFLNNLHNTILEKNWTFNYPKMTTTFGSYIYFPILQIDSFKNSDSLKII
                     VAAGKYDGIANDDYKVNIYKRYDDKYKNEEDVKNSYSIGIGRVVSVENNRTIIYIKSS
                     STVPIEPKDLANIYCQTPVSAKKNKFVPLLDIGINLLDNTKEKLLLSRRFLLYYDDEK
                     IHFELIKNLKVELFYVQNAYAEDTLKPNNTLAQKATEGIFKGVNIIKAFDSTNTKHIV
                     AFINFMKAYPGKYIGNNFSFSEIYATWLLNNTPMANDDLLLYIAQTEKNKRAEIAYAL
                     KNQIKQNNIIEKWVTLTLNAFDKNDLLNVRIALMGFAAVYESEKNIDVGGWLSFLNGL
                     FQYKTGSVSLADTALKKAEKFFTKSNNKEGLTITKKALQKIHTNNKVSLEVQLGEFAS
                     YSVAMHPSGKYYATNDANNTIRIWDLHLGKTIHTFQPQNEIVYSIAYSPNGRYFATCS
                     DNKTIKIYSTFDYAVITTINTSNTEYCIAFSPNNKEIASGGYDSLIKVWNFITGEKLY
                     SLNKHKGAVTQLCYVGRNEDILFSSGTDSMVYRWNLQTKHDVNWYRKKAKLLNMKVSY
                     NGNYLFYTANDSSINVWNLYKNKFYFKEKIAVAQYGDTKFYNEPDFSPDGSLLTYANQ
                     QNSVVLVDLNTGYLKAITKTANKYAYLNTLNFTTDQMGIFATYPYISKTKLFDISGYK
                     NIHSFNTNVELKINKQYTNPNITLQFSAKDDALFTVGERIVKFDFSNNSTHQLFYAAQ
                     AIYNTKFMINDSISFVSGSLSISLFNHLSKKTISSIELTTKDTLAQYFFNEKNNIIYL
                     ISKKGSIEGYRVNNFIIEPAIVFSATINLKENKFINLVKFYSDNNDLVISTTGKIADM
                     YRVNLSSSKIITLPSIKKFYDFIVAKNCFYFNNNNGTITKNNVKNLKVEKTYTLTNVN
                     DVAGFLQLSSNKKLLAAYYNETNIAVIDVEKEKLLYTIKAHDVIGMGLAFSNNSKYLA
                     TGGFDSKICLFNASTGEKLLNIFTPAGLDFVAADTTGYYMASKNSLEGLSFKLNENIY
                     PFEQFDLEFNRPDKVLAKSGLGDTTLIAAYKKAVSKRLKKNNSSSINLSQIEQLPSLS
                     ITDNTDVSYFTNLNFTELTINCYHEKQPIKALHVLVNNNPVYGFAGKNMGNQSHDTSI
                     TIKIPLAVGKNFIKLFCTNSSGQNSLQQTFEVRCNSNADTSAKTWFIGIGVSNYKDKD
                     MNLHYAAKDVRDLATTFTKDKNNVVVDTLIDSKVTIENILTLRKKLLTANPNDKVIIA
                     ITGHGMLDKNYDFYYGTYDIDFNNPNKRGLKYEMLEKILDSVPAQKKLLLIDACHSGA
                     LDKEELKKDSSKLFVDNTDTNKTTVVTRSTIKKKKLSKVSLNNTFELMQNLFSDFTNN
                     NGSVVISAAGGLEFAFESATWNNGVFTYCIRKALENFEADEEGDFNSKTSISELMKYV
                     SENVTRLTNGQQKPTSRREVLDFDWEL"
     gene            complement(8129..8860)
                     /locus_tag="PLY81_01680"
     CDS             complement(8129..8860)
                     /locus_tag="PLY81_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF28972.1"
                     /translation="MKNKDKIIPSREHYYLEGPKHRVNELVWVWSIFVEFIGAIRKLH
                     FTGPCITVFGSARFKEDNEYYIAAREFGKRIAQLGFTTMTGGGPGIMEAANRGAFENG
                     GYSIGCNIKLPFEQKENPYINKSYTFNYFFSRKVLLVKYSYAFIIMPGGFGTMDELFE
                     TLTLIQTKTINNFPVVLYGKKYFQPLINYIEEMIEQKTISKEDLNLLLVTDDFEEAIN
                     HIKAYVHKHYKVKPRKRLWWLFEKK"
     gene            8960..9730
                     /locus_tag="PLY81_01685"
     CDS             8960..9730
                     /locus_tag="PLY81_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNF28973.1"
                     /translation="MRIISYNVNGIRAAIKKGFIDWLKTNPADIICLQETKATISDID
                     LQPFQELGFEAYFFSAQKRGYSGVAVFTKLKPNKVVYGTQIEQSDFEGRVIQLYFKNF
                     ILINAYFPSGTSGDERQTYKYQWLNEMYDYVQALGKEHKNIVLVGDYNIAHTEIDIHD
                     PKGNKNSSGFLPEERAWLTKFLHTGWIDSFRFKNPDTIGGYTWWSQRFPSVRLQNKGW
                     RIDYICVTNALKNKIKTASIFADIKHSDHCPIFLELSV"
     gene            9761..10477
                     /gene="kdsB"
                     /locus_tag="PLY81_01690"
     CDS             9761..10477
                     /gene="kdsB"
                     /locus_tag="PLY81_01690"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNF28974.1"
                     /translation="MKISAFIPARYAATRFPAKLMQMLGNKTVIRHTYDNTLATGLFN
                     EVWVVTDSAIIYNEIVNNGGRAVMSVQEHESGSDRIAEAVKNIDVDIIVNVQGDEPFV
                     KKEPLEKLLNVFEDSSVQVASLMQVLKNEQQINDPNFVKVAVDKNMNSLLFSRSVIPY
                     HRDKNIAPTYYEHIGVYAFTKQALLHFTQWEITPLEAAEKIECLRYVENGIALKMVVT
                     DYMGIEIDTPEDLINATKFL"
     gene            complement(10479..>10851)
                     /locus_tag="PLY81_01695"
     CDS             complement(10479..>10851)
                     /locus_tag="PLY81_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28975.1"
                     /translation="VEVTTRKFYYGSFAFDGITSFTSYDGKFGKNDLHTESLVIDYDA
                     LQQEREFYAPKYDIVENKESRVPDYRVLLHWSGTIKTTTNGATDIEFYTSDLGGKYTA
                     IIQGITNTGNPVKSMVEFTVK"
BASE COUNT         4098 a         1524 c         1745 g         3484 t
ORIGIN      
        1 gtaattctac cccatagcca aaactatgtg ttgtcccaaa gctcccaatt ggcatagata
       61 acgcagggcc agcaccaaag ctaaaacctt tgctagatga agactctttt ttttcttgtg
      121 cattagccgt aaagcctact aaggcagcta aagcaataat aaaaattttt ttcatgttgt
      181 taaaatttta taaaagggtt aaataataaa gccgaaggta aagtaattaa tacttaattc
      241 aatacccact taagggtatt ttaaaatatg tgcataaaag aaacagatgt ataaatttta
      301 ttaacactat aaataaatat tttgttttct cttttattat tattgaaaca attaccaaca
      361 aaacaaattg caagtaatac ttttacactt cttttaaaaa aatgcttgtg gcaaaacgta
      421 catctaaatt aattgctaat gataactttg ataatagtaa gattatagta caaggtgcaa
      481 gagaaaataa tttaaaaaac atagatattg ctattccaaa aaataaactt gtggttttta
      541 caggtgtaag tgggagtggt aaaagttctt tagcatttga tactatttat aatgaggggc
      601 aaagaaggta tatggaaagc tttggtgctt atgcaagaca atttttaggc aatatggaac
      661 ggcccgatgt agataaaatt tctggtcttt ctcctgttat ttctatagaa caaaaaacaa
      721 caaataaaaa cccaagaagt acagtaggca cagtaactga attgtatgat tttttgcgtt
      781 tactatttgc ccgtattgga gaagcttata gctataatac agggaaaaaa atggttaaac
      841 taagcgaaga ggaaattata caaaccattt ttgaaaagtt taaagaagag aagattttaa
      901 ttttagcacc attggttcgt ggcagaaaag gacactatag agaactgttt gaagaaacca
      961 gaaaaaaagg atttttaaaa gtaagaattg atggagaagt aaaagactta acacctaaac
     1021 tacaagtgga tagatataaa gtacacaata ttgaaattgt agtggataga cttgaggtta
     1081 caatgaatat gaaattaagg ctaagccaaa gcatacaaca aagtttaaaa attggtaaag
     1141 atttaatatt tgttgcatct gccaatcaaa tatccaacaa caaagatgct gtttttagta
     1201 aacagttaat gtgtgtggat actggtattt cttacgaaga accttcacca aatacatttt
     1261 cattcaactc gccttatggt gcttgcccaa cctgtaaagg cttaggagtt gaatataata
     1321 ttaatataga tgctgtttta cccaataaat ccaaaacaat aaaagaagga ggcattgtgc
     1381 ctttgggaga agaaagagat gctagcgttt ttaaacaagt tgttgatttt gcaaaaaaaa
     1441 ataaaattag cttagatact cctttagaaa aactttcaga ccaacaacta aacctaatac
     1501 tctttggcga taaaaatatt acacaggatt tagtaataaa catgaacgat gaaaatattc
     1561 ctaacgaata cactggcagt tacgaaggaa ttattcctat gttaaaacga tggtttacat
     1621 ctaccaacag tactgatttt ttacaaggtt gggtagaaaa attcatgact caaggtatat
     1681 gcagtagttg taatggaaaa aaattaagaa aagaaagttt atggataaaa ataaacaaca
     1741 aaaacataag cgatatcagc gaaatgaatt tagataaact tatgctttgg cttaatacta
     1801 ttgaacataa tttaaattca aaacaaaaca taattgcaaa agatatttta aaagaaataa
     1861 gagaacgatt gcaattttta ttagatgttg gcctaagcta tttatcgtta aacagaagca
     1921 gtaaaagcct tagtggtggc gaagctcagc gaattagatt ggcaacacaa ataggtagta
     1981 aactacaggg tataacctat attttagacg aaccaagtat tggacttcat caacgagata
     2041 atcataaact aatagattct ttaaaaaatt taagagatat tggtaatagc attttagtgg
     2101 tagaacatga taaagacatt atgttagcta gcgattattt ggtagatatt ggaccaaagg
     2161 ctggtaagca tggaggacaa attgtagcat ttggcacacc taaagaaata ttaaaaagca
     2221 acagcgatac agcacaatat ttatctggca aaaaggctat ctctattccc aatgtaagaa
     2281 ggaagggtaa tggtaaattt attgaactaa ttggtgctag tggcaataat ttaaaaacca
     2341 taacaaccaa acttccatta ggaaatttaa ttgttataac aggtgtaagc ggtagtggta
     2401 aaagcacttt aattaatgaa acactgtatc caattttaag caaacattgt tataaaagta
     2461 atattccaat taaggaattt aaaagtatta atggtttaga gaatatagat aaagtaattg
     2521 aaatagacca aagcccaatt ggcagaacac caagaagcaa ccctgccact tattgtggtt
     2581 tttttacgga aattagaact ttgtttgcca gtgtaccaga agcaaaaata agaggttata
     2641 ctgcaggcag attttcattt aatgtaaaaa caggcagatg cgaagtttgt gaaggcggcg
     2701 gaatgaaagt gattgaaatg aattttttac cagatgttta tgtacactgc gaaaagtgta
     2761 atggtaaaag atataacaga gaaacattag aaataagata taaaggaaaa agcattagcg
     2821 atgtgctaaa catgacagtt gatgaagcct gtgaattttt taatgcagtg ccctggcttt
     2881 acagaaaaat aaaagttcta caagatgtag gcttgggcta tttaacgctt gggcaaagtg
     2941 ctgtaacact tagtggtggc gaagctcaac gtgtaaaatt atctacagaa ttaggcaaaa
     3001 aagacacagg caaaactttt tatattcttg atgagccaac tactgggtta cattttcaag
     3061 atatacaatt gttaatggaa gtggtaaaca aattggtgga tagaggcaat actgttttaa
     3121 ttattgaaca caacttagat gttataaaac ttgcagatta tattattgat ttagggcctg
     3181 aaggaggcga tggtggaggt gaaatattat ttgaaggcac acccgaagaa atgattctta
     3241 ataaagaaag ttatacagca aagtttttaa aacaagagct atcaaactaa gtttaaactg
     3301 atattaacaa ccattatttc ggaaaaattg tattttgtaa ctgtatccct ttttgtttta
     3361 tgaaataagt ttacctgttt tatatgaaat tagttttact ttctactttt ttaatttttc
     3421 atctctttgt ttttagccaa acaaattcag aaattgattt taaagcacat tttgaaaaag
     3481 gcaccaactt gctacttgct gaaaaatatg attctgcatt atactacttt aatttatcag
     3541 aaaaaattgc tgaaaagcaa aataaatatt cagacgataa atactcctct ttaaatataa
     3601 ataaaggtat ttgttacatg tatttacaag agccggataa tgctcattat tatatgtaca
     3661 agggactttt gaatgctaga aaatttaaac atattattcc cgaatccaat gctattctat
     3721 ttttaaataa cttacataat actattctag aaaaaaactg gacttttaat tacccaaaaa
     3781 tgaccaccac ctttggcagt tatatttatt tccctattct tcaaatagat agttttaaaa
     3841 actctgacag cttaaaaata atagttgctg caggcaaata cgatggtata gcaaatgacg
     3901 attacaaagt aaatatttat aaaagatacg atgataaata taaaaatgaa gaagatgtaa
     3961 aaaatagtta ttctataggt ataggaagag ttgtaagtgt tgaaaataac agaaccatta
     4021 tatatattaa atcatcctct actgtaccaa tagagcctaa agatttggct aatatttatt
     4081 gccaaacccc tgtgagtgca aaaaaaaata agtttgtgcc attgttagat attggaataa
     4141 acttgttgga taatacaaaa gaaaaactat tattaagccg tagatttttg ttgtattacg
     4201 atgatgaaaa aattcatttt gagctaataa aaaacttaaa agttgaatta ttttatgttc
     4261 aaaatgctta cgcagaagat actttaaaac caaataatac tttagcccaa aaagcaacag
     4321 aaggaatttt taaaggcgtt aatattatta aagcctttga tagcaccaac actaaacata
     4381 tagttgcttt tataaacttt atgaaagcat acccaggtaa gtatattggc aataattttt
     4441 cattttctga aatttatgct acctggcttt taaataatac cccaatggcc aacgatgatc
     4501 ttttactcta tattgctcaa acagaaaaaa ataaaagggc cgaaattgct tatgctttaa
     4561 agaatcaaat taaacagaat aatattattg aaaaatgggt aaccctaaca ctaaatgctt
     4621 ttgataaaaa cgatttgcta aatgttagaa ttgctttaat gggttttgcg gcagtgtatg
     4681 aaagtgaaaa aaatattgat gttggtgggt ggctaagttt tttaaatggt ttgtttcaat
     4741 acaaaacagg aagtgtttcc ttggcagata ctgctttaaa aaaggcagaa aagtttttta
     4801 ccaaatctaa taacaaagaa ggtctaacaa taaccaaaaa ggcattacaa aaaatacaca
     4861 ctaataataa agtaagttta gaagtacagc ttggagagtt tgcaagctat agtgttgcaa
     4921 tgcatccatc tggcaaatat tatgcaacca acgatgcaaa taatactatc agaatttggg
     4981 atttacatct aggaaaaaca attcacactt ttcaaccaca aaatgaaata gtttattcca
     5041 tagcatacag ccccaatggc cgttattttg caacctgtag tgataataaa accattaaaa
     5101 tttatagcac atttgattat gcagtaatta ctacaattaa tacctctaat actgaatact
     5161 gtattgcttt ttctcctaat aataaagaaa tagcaagtgg cggatatgac tctttaataa
     5221 aagtgtggaa ttttataact ggcgaaaaac tatacagttt aaataaacac aaaggtgcag
     5281 ttacacagct ttgctatgtt ggtagaaacg aagatatttt atttagtagt ggtacggata
     5341 gtatggttta tcgttggaat ttacaaacaa aacatgatgt gaattggtac agaaaaaaag
     5401 caaagctttt aaatatgaaa gttagctaca acggtaatta tttattttat actgccaacg
     5461 atagctctat taatgtttgg aatttatata aaaataaatt ttattttaaa gaaaaaatag
     5521 ctgttgccca atacggggat acaaagtttt ataacgagcc cgatttttca ccagatggca
     5581 gtttacttac ttatgccaac caacaaaaca gtgttgtatt agttgaccta aacacaggct
     5641 atttaaaagc aataacaaaa actgcaaata aatatgctta tttaaataca ttaaatttta
     5701 ctaccgatca aatgggaatt tttgcaacct acccatacat aagtaaaaca aaactctttg
     5761 atattagtgg atataaaaac attcacagtt ttaatacaaa tgtggagtta aaaataaaca
     5821 aacaatatac caatccaaat ataaccttac agtttagtgc aaaagatgat gctctattta
     5881 ccgttgggga aagaatagta aaattcgatt tttccaacaa tagcacacac caactttttt
     5941 atgcagcaca ggctatttat aacacaaaat tcatgattaa tgattctatt tcatttgtaa
     6001 gtggcagcct ttctatctct ttgtttaacc atttatctaa aaaaactatt tcatctattg
     6061 aattaacaac caaagacact ttagcacaat attttttcaa cgaaaaaaat aatattattt
     6121 atttaattag caaaaagggt agcatagaag gctatagggt taataatttt attatagagc
     6181 ccgccattgt attttctgct acaataaatt taaaggaaaa taaatttatt aacctcgtaa
     6241 aattctatag tgataataat gatttagtta taagtacaac aggtaaaatt gctgatatgt
     6301 atagagtaaa tttatctagt tcaaaaatta taacactccc gtctataaaa aaattttacg
     6361 attttattgt tgctaaaaac tgtttttatt tcaataacaa taatggtact ataacaaaaa
     6421 ataatgtaaa aaatttaaag gtagaaaaaa cttatacatt aactaatgta aatgatgttg
     6481 ctggcttttt acaattatct tctaataaaa aactattggc tgcttattat aacgaaacaa
     6541 atattgcagt aatagatgtt gaaaaagaaa agctacttta cactattaag gctcatgatg
     6601 taattggaat gggtttggct tttagcaata atagcaagta ccttgcaacg ggtggtttcg
     6661 attctaaaat atgcttattt aatgcaagca caggagaaaa gcttttaaat atttttacac
     6721 ctgctgggtt agattttgta gctgcagata caacaggata ttacatggca agtaaaaact
     6781 ctttagaggg attaagtttt aaattaaatg aaaatatata tccatttgaa cagtttgatt
     6841 tggagtttaa taggccagat aaggtgttgg caaagtctgg tttgggtgat actacattaa
     6901 ttgcagcata caaaaaagca gtttcaaaaa gattaaagaa aaacaactct tcaagtatta
     6961 acctatcgca aattgagcaa ttaccctctt taagcattac agacaataca gatgtaagct
     7021 actttaccaa tttaaatttt accgagttaa caattaattg ctatcacgaa aaacaaccta
     7081 taaaggcatt gcatgtttta gtaaacaata acccagttta tggttttgca ggaaaaaata
     7141 tgggcaacca aagccacgat acttctatta ccataaaaat tcctttggcg gtaggcaaaa
     7201 attttatcaa acttttttgc actaattcct ctggtcaaaa ctctttacag caaacctttg
     7261 aagtaagatg taacagcaat gcagatacct cagctaaaac atggtttata ggtattggtg
     7321 tttctaatta taaggataaa gatatgaacc tgcactatgc agctaaagat gttagagatt
     7381 tagcaaccac ttttacaaaa gataaaaata atgtagtagt agatacttta atagatagca
     7441 aagtaaccat tgaaaatatt cttaccctta gaaaaaaatt attaactgcc aacccaaacg
     7501 ataaagtaat tattgctatt acaggtcatg gtatgttaga taaaaactat gatttttatt
     7561 atggtactta cgatattgat tttaataacc ctaacaaaag aggtttaaaa tatgaaatgt
     7621 tagaaaaaat tctagacagc gttcctgcac aaaaaaaatt attactaata gatgcttgtc
     7681 atagtggagc tttagataaa gaagaactta aaaaagactc ttcaaaatta tttgtggata
     7741 atacagatac aaataaaaca actgtggtaa ctagaagcac aataaaaaag aaaaagctta
     7801 gtaaagtaag tttaaacaac acgtttgaat taatgcaaaa tcttttttct gattttacca
     7861 acaacaacgg aagtgttgta atatctgctg caggtggttt agagtttgct tttgaaagtg
     7921 caacatggaa taatggtgtt tttacttatt gtataagaaa agctttagaa aattttgaag
     7981 cagatgaaga aggagacttt aatagtaaaa cttctattag cgagctaatg aaatatgtga
     8041 gtgaaaatgt aaccaggtta accaatgggc aacaaaagcc aacctctcgt agagaggttt
     8101 tagattttga ttgggaacta taaaccattt attttttttc gaataaccac caaagccttt
     8161 tgcgtggttt taccttataa tgcttatgta cataggcctt tatatgattt atagcttcct
     8221 caaaatcatc ggttactaaa agtaaattca aatcttcttt agatattgtt ttttgctcta
     8281 tcatttcttc aatataattt ataaggggtt gaaaatattt tttaccatat aaaacaaccg
     8341 gaaaattatt tattgttttt gtttgaatta gagttaaggt ttcaaacaac tcatccattg
     8401 taccaaaccc accaggcatt attataaaag cataagaata ttttacaagt aaaaccttac
     8461 gagaaaaaaa ataattaaac gtgtacgatt tgtttatgta tggattttct ttttgctcaa
     8521 aaggtagttt aatattacag ccaatactat atccaccatt ttcaaatgca cccctgttgg
     8581 ctgcttccat aattcctggt cctccaccag tcatagtggt aaaacctaat tgtgcaattc
     8641 tttttccaaa ctctctggct gcaatgtaat attcattatc ttctttaaat ctggcactac
     8701 caaaaacagt aatacaggga cccgtaaaat gtaattttct tattgctcca ataaattcaa
     8761 caaaaatact ccaaacccat acaagctcat taactctatg ttttgggcct tctaagtaat
     8821 aatgttctct acttggtata attttatctt tgtttttcat ttttgtatat ttcttattta
     8881 ataattttta tactcaataa taatacctta attaatttat attaaatttt atattaaaac
     8941 taactattaa atttatacaa tgagaattat ttcatacaat gtaaatggta taagggctgc
     9001 aataaaaaag ggatttatag attggctaaa aactaaccct gcagatatta tttgtttgca
     9061 agaaacgaag gcaaccataa gtgatattga cctacaaccc tttcaggaat tgggctttga
     9121 ggcctatttt ttttctgctc aaaaaagggg atatagtggt gttgctgttt ttacaaaatt
     9181 aaaaccaaac aaagttgttt atggtacaca aatagaacaa agcgattttg agggtagagt
     9241 aattcaactt tatttcaaaa actttatttt aattaatgca tattttccaa gtggcaccag
     9301 tggggatgaa aggcaaacct ataaatacca atggttaaat gaaatgtatg attatgtaca
     9361 agctttgggt aaagagcata aaaacattgt acttgttgga gattataata ttgcccatac
     9421 cgaaatagat attcacgacc ctaaaggcaa taaaaattca tctggttttt taccagagga
     9481 acgtgcctgg cttacaaaat ttttacatac aggttggatt gatagtttta gattcaaaaa
     9541 ccctgataca ataggtggat atacatggtg gagccagcgt tttccaagtg tgcgtttaca
     9601 aaacaagggt tggcgtatag attatatttg tgtaacaaat gctcttaaaa ataaaattaa
     9661 aactgcaagt atttttgcag atattaaaca tagcgaccac tgtcctatat ttttagaatt
     9721 aagtgtttaa taatttaaaa gaagttaata caaaatattt atgaaaattt ctgcatttat
     9781 tcctgcacgt tatgctgcta ctcgttttcc tgcaaagctt atgcaaatgt tgggtaacaa
     9841 aacagttatt aggcacactt atgataatac acttgccaca ggcttgttta acgaggtttg
     9901 ggtagttaca gatagtgcta ttatttataa tgaaattgta aataatggag gtagggctgt
     9961 tatgagtgtg caagaacacg aaagtggtag cgatagaatt gctgaagctg taaaaaatat
    10021 agacgtagat ataattgtaa atgtgcaggg cgatgagcca tttgttaaaa aagaaccttt
    10081 agaaaaatta ttaaatgttt ttgaagacag ctctgttcaa gttgcaagct taatgcaggt
    10141 tttaaaaaat gaacaacaaa taaacgatcc taattttgtt aaggttgctg ttgataaaaa
    10201 tatgaatagt ttgcttttta gccgttctgt tataccttat cacagagata aaaatattgc
    10261 tccaacatat tatgaacata ttggtgtata tgcttttacc aaacaagcat tattacattt
    10321 tacacagtgg gaaattacac cattagaggc tgctgaaaaa atagagtgtt tacgctatgt
    10381 agaaaatggt atagcattaa aaatggttgt taccgattac atgggtattg aaattgatac
    10441 ccctgaagat ttaattaatg ctacaaaatt tttatagttt actttactgt aaactcaacc
    10501 atagatttta ctgggttgcc tgtattggta ataccctgaa taatggctgt atattttcca
    10561 cctaaatcag aggtataaaa ttctatatct gttgccccat ttgttgttgt tttaatagtg
    10621 ccagaccaat gtagcaatac tctataatct ggcaccctgc tttctttatt ttctactata
    10681 tcatatttag gtgcataaaa ttctctttct tgttgaagtg catcatagtc tatcactaag
    10741 gattcagtat gtaaatcatt ttttccaaac tttccatcat aacttgtaaa gcttgtaata
    10801 ccatcaaagg caaaactgcc ataataaaat tttcttgttg ttacttctac t
//
LOCUS       DAOQHP010000025        10733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3075_length_10733_cov_3.689549, whole genome shotgun sequence.
ACCESSION   DAOQHP010000025 DAOQHP010000000
VERSION     DAOQHP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_10733_cov_3.689549"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3515
                     /locus_tag="PLY81_01700"
     CDS             <1..3515
                     /locus_tag="PLY81_01700"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNF28976.1"
                     /translation="TVVVNNGNYQSAPYGLRLVNHNNSGGYPASTSKATSPTINLSGT
                     SNVELSFKLYTHTVHANNTCYTFNVEFSNDNGSTWTNVYSKTSQYLYNTFGLNTWNTI
                     TIPVPNNYYNANFKYRVSGVQQSSCNFNAYLYLDDIKVQTNTPCLPCPTNNDVAAGAN
                     QDQCNNANFTMSASAPPQGSTGTWSIVSGTATIANINSPTTAVTVTGSPVTLKWTITT
                     PGCITPAFDTVTLTNTVTTTANAGSDQSICGSNSFTMSANAVGGGETGTWTVISGAAT
                     ISNVNSPTTLVNVTTSPAVLRWTISKNGCSTYDEVTLTVTNGSSLLGDFVWWDINDNG
                     IQDAGEPGIDGVTVKLYADANSDNIPDSNVPLFTTTTNASGQYLFNNLCTGKYIVSIV
                     TPDGYEQGTTTGTSSDPNNGSEYDNNGVAIYNGTEIRTNNVNFSLNNPRVDFGLKGKL
                     NLGNLVWMDQCNYGTKDNSETGVNGVTVYLYRDANADNLPDGSAIKTTTTNVNGIYNF
                     GTLAPGKYIVGILLPTGYASTVTTINSSNPNSDTDNDNNGIRTSSGILYSNFITLTFG
                     GEPNTGIDGDGTNGNLTLDFGIAKDTDGDRIADIIDIDDDNDGITDVNESGGFNPLGD
                     CDNDCIPNYLDNTPGNGCPEWSDCNGDGINDFYDWDRDGIINSLDLDSDNDGILDVME
                     ARPSANAFNNHSNGMITGTDTDANGLLSSADNGSGFNNPHLNGLIPHDLDRDGKPNFL
                     DLDSDGDGITDLTEALGVYSSTGVVTGTDEDGDGVRGENFGNNGANVADNINGFGAKG
                     VTLLDSDNDGRPNAYDIDSDNDGITDNTEAQATCSFKMPLYSDCDNDGVDDAYDIGGC
                     ASCTRTSGGLTPFDKDGDSTPDYLDLDTDNDSAPDIYEGHTIKTVNYWIGATGDADKD
                     GLMDYFDGYNIITATDGLYWRNVINNNMGNFGSWDTGVGSTGSISQLPKSRPGACNVG
                     DRDWRDFYILPVTIVEFKGNITSDAIAKLNWSVTNEININHYDLERSTNGSEFSKVAQ
                     VNAINASSSSAISNYNANDNVAGLNGIIYYRLKIVENDGTIKYSNVINFKLSQSTRTV
                     VSLYPNPAVNNFTVKITSPKDETVQVRVFDMLGKVLLSQQSTIVVGINNLSFNNISKL
                     STGTYTVQIIMSNQVFTEKLVVTK"
     gene            complement(3635..4897)
                     /locus_tag="PLY81_01705"
     CDS             complement(3635..4897)
                     /locus_tag="PLY81_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF28977.1"
                     /translation="MALHFPIVSWAIGASIYEVNIRQYTTEGTIKSFQNNLLRLKEMG
                     ITILWFMPLTPISKEKRQGSLGSYYACSAYTKVNDEFGTAEDFKELVNQAHALGLKVI
                     IDWVANHTGFNHEWAITNSDWFIKDEHGNFTEKNGWIDVIDLDYTNINMRSAMINAMQ
                     YWINEFDIDGFRCDMAHLVPLSFWYEARKHCDVIKELFWLAECDEDEYLNVFDANYAW
                     QLMHISENYCKQKSTLNQIKDLLSTYNGLPTNTYKLLFTSNHDENSWNGTEYEKYGDA
                     AKAFAVLSCTFKGISLLYSGQENPNLKRLAFFEKDTIQWNKTFLLEQFYTKLLNLRKN
                     KAISQGQTNFISISNNNILVYIRKYEENIVIVLLNLSQENKVKFMLEHEWLSGNFKNL
                     FSDFEFSFNYTSNFELQAYEARVYVKIN"
     gene            complement(4964..6688)
                     /locus_tag="PLY81_01710"
     CDS             complement(4964..6688)
                     /locus_tag="PLY81_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNF28978.1"
                     /translation="MKSSIDWIVLIVTLATIIAYGVYKSRTSKNLEGYFLSNRNMPWY
                     LILLSIMGTQASAITFLSAPGQAFTDGMRFVQYYFGLPLAMVALCITFVPLFGKLKIF
                     TAYEFLEQRFDVKTRAFTSFLFLLSRGLSTGISIYAPSIILSSLMGWDIFITNIIMGG
                     LLIIYTVSGGAKAVAYTQQIQLIIIFAAMFFAGYTIINLLPKNIGFIDALNISGGSNK
                     MNIITSGFENGNYNWKDKYNIISGVIGGFFLALSYFGTDQSQVGRYLTAKNTTESRLG
                     LLMNGLVKVPMQFFILLIGVLLFAFYQFKPQPIFFNPVQEQKLLQSNKKDSAILLQNE
                     FDKLNTLKTETLKNYSSNKEQTIKTIHHINDSASRIKNTYINLFNTANIEGDKNDTNY
                     IFLRFVVDYLPVGLVGLIIAIIFLAAWGSIAAALNSLAACTIIDFHKRFSKKKMIDET
                     EQAIKDYRYSKIYTLLWGIFCIVIAETVVGMGSLIEAVNVLGSLFYGVILGIFLVGFY
                     IKKVKGNAVFVAAIISEIAIIGLFVLDKNGIIGLGFLWLNVVGALLVMLLSFILQYVI
                     HGLPKSSK"
     gene            complement(6826..9336)
                     /locus_tag="PLY81_01715"
     CDS             complement(6826..9336)
                     /locus_tag="PLY81_01715"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNF28979.1"
                     /translation="MKKILVSSLLLITTFLKAQTNSAEIFIELKKLNVLGSVLYIAAH
                     PDDENNTLLPYLAKEKLYRTAYLSLTRGDGGQNLIGDEQGIELGMIRTQELLAARRID
                     GGEQYFTNAYEFGYSKTAEETLNKWNRLQLLYEIVWNIRKFQPDIIINRFPQDGRAGH
                     GHHAASAILAQEAFIAAADITKFPEQFTLGVQPWQAKRLLWNTFNFGNNNTTNNNQLK
                     LEVGNYNPLLGLSYGEVGGIARTMHKSQGEGRPKRKGNITEYFAYVNGDSATTDIMNR
                     VNTTWSRLEGTSIIQQKINEVIAEYNFANPQSSVSKLVTIYNLLSNYPTKNNWVTLKI
                     KEIENLIIKCSGLFIEATSSEEAVVQGQKATFNFFSNNRSDSNIKLSKISFSISNNNT
                     FDSVINLQLLKNSNYSFSKECIIPLTQPVTQPYWLTKPILKNMFHPSNQAMLGEAENS
                     PTYIAKFFLNIDGTDFTLNVPIQFKMVHPVRGEIYEPLIIVPPITVNLTPNNILLHIQ
                     QNKKIVNSKNIQLQVTSNINALKTSVTISLHQANNIVYAKDTILDLKNAESYAFNIAI
                     SNLKNYTFNEVITPSVQINYQTKEQQYTQDLKNISYEHIPNLHYFFTNETKFINEKII
                     TKPLKVGYINGAGDKVAEAIEQMGYIVTQLTEKDLNIETLKQYNAIVIGIRAYNIHEY
                     LTDKNNVLNAFVKNGGNLIVQYIKSNSIGTKAIQSGPYSFTVYSRSRITEEDAKVKFL
                     LPNHTVLNYPNKISSADFENWVQERSTYQAEKFDSNFECILGMNDTGEKETNSSLLIA
                     KYGKGNYVYCSLALFRQLPAGIAGSYKLLANMLELEKH"
     gene            complement(9379..>10733)
                     /locus_tag="PLY81_01720"
     CDS             complement(9379..>10733)
                     /locus_tag="PLY81_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc carboxypeptidase"
                     /protein_id="HNF28980.1"
                     /translation="DNKYNIEAIKDLLNKNGILYGTIDYKGLKGYCYQLNKDAEVKPL
                     TNTIAVSTCQPKGILANILLEPKSKLNDSATYDITAWSLGYAYGVNTYAIKEKKEINY
                     AVTLPATIRNYSSNYGYLIQYNSINSVKVLAHLLKKGIKVRFAEKPFVYNGKNYERGT
                     LIIIKTGNDINLINKEINFAANKFDINADIVTTGFMDKGIDFGSPHVKLINTKKILLV
                     TGESVSSLAAGEVWNFFDKQLNYPITLVNATELNRINFKNYQVIILPDGYYKFSSDKN
                     TVDKIKEFVSTGGKLIALESAVNQLAQLDVGFKLKTNPDKEPEKTDYTLLKKYADREK
                     DYLPSSIPGAIYKIDLDNTHPLAFGYDSIYYTLKLDATIYEFMKEGWNVGYLKKENYV
                     TGFAGYKVKENLKDGTVIGATEYGRGNIIFFADNPIFRMFWENGKLMLCNAVFMVGQE
                     "
BASE COUNT         3608 a         1647 c         1609 g         3869 t
ORIGIN      
        1 ctactgttgt tgtaaacaat ggtaattacc aatctgcccc ttatgggtta agattagtaa
       61 accacaacaa tagtggtgga tatcctgcaa gcacttctaa agctacatct ccaactatta
      121 acttaagtgg tacaagcaat gtagagttaa gttttaaact ttacacacat acagtgcatg
      181 ccaataatac ttgttatact tttaatgttg aattttcaaa tgataatgga agtacttgga
      241 caaatgtgta ttctaaaaca tctcaatatt tatacaatac gtttggatta aatacttgga
      301 atactattac aatacctgtt ccaaacaatt actataatgc taattttaaa tatagagtaa
      361 gtggtgttca gcaaagtagt tgtaatttta atgcttattt atatttagat gatattaagg
      421 ttcaaactaa tacaccttgt ttgccttgcc caacaaataa cgatgtagct gcaggtgcca
      481 atcaagatca gtgtaacaat gccaacttta caatgtctgc ttctgcacca ccacaaggct
      541 caacaggtac atggagcatt gtaagtggta ctgcaacaat tgccaacatt aattcaccta
      601 caacagctgt tactgtaact ggctcacctg taactttaaa atggacaata actacaccag
      661 gttgcataac acctgctttt gatacagtta ctttaactaa tacagttact acaactgcaa
      721 atgccggttc tgaccaatca atttgtggta gtaattcatt tactatgagt gcaaatgcag
      781 ttggtggagg cgaaacaggt acatggactg taataagtgg tgctgcaaca atttctaatg
      841 taaattctcc aacaacttta gtaaatgtta caacttctcc agcagtttta cgttggacaa
      901 ttagcaaaaa tggttgttct acttacgatg aagtaacttt aactgtaacc aatggctcaa
      961 gcctattagg tgattttgta tggtgggata ttaacgataa tggaattcaa gatgctggtg
     1021 aacctggtat tgatggagta actgttaagt tatatgcaga tgctaattct gacaatatac
     1081 ctgatagtaa tgttccatta tttactacaa caactaatgc ttcaggccaa tatttattca
     1141 ataatctttg tactggaaaa tacattgtaa gtattgtaac gccagatgga tatgaacaag
     1201 gcactacaac tggtacaagc tcagatccta ataatggtag tgagtacgat aataatggtg
     1261 tagctattta taatggtaca gaaataagaa ctaataatgt taacttcagc cttaacaatc
     1321 ctagagttga ttttggattg aaaggaaaat taaacttagg taatttagta tggatggatc
     1381 agtgtaatta tggtactaaa gataattctg aaactggcgt aaatggtgtg actgtttatt
     1441 tatatagaga tgctaatgca gataatttac ctgatggttc tgctataaaa acaacaacaa
     1501 caaatgtaaa tggcatttac aactttggaa ctttagctcc tggtaaatat attgtaggta
     1561 tattactacc tactggctat gcttcaacag ttactactat aaatagttca aaccctaata
     1621 gcgatactga taatgacaat aatggtataa gaacatctag cggaatttta tacagcaact
     1681 ttattacatt aacatttgga ggagaaccta atacaggcat tgatggtgat ggtactaacg
     1741 gaaacttaac tttagatttt ggtattgcta aagatactga tggagataga attgctgata
     1801 ttattgatat agatgatgac aatgatggaa taactgatgt aaatgaaagt ggtggattta
     1861 atccattagg cgattgtgat aatgattgta ttcctaacta tttagataat acccctggta
     1921 atggttgccc tgaatggagt gattgtaatg gtgatggtat aaatgacttt tatgactggg
     1981 atagagatgg tatcattaac tcattagact tagacagtga taatgatggt attttagatg
     2041 taatggaagc aagaccatca gccaatgcat ttaataacca tagtaatggt atgataacag
     2101 gtactgatac cgatgcaaat ggtttattga gttctgcaga taatggctct gggtttaata
     2161 accctcattt gaatggctta ataccacacg atttagacag agacggtaaa cctaactttt
     2221 tagatttaga tagcgatgga gatggtataa ctgatttaac tgaagcatta ggtgtatatt
     2281 catctacagg tgtagtaact ggcaccgatg aagatggtga tggtgtaaga ggtgaaaact
     2341 ttggtaacaa tggagcaaat gtagcagata atattaatgg ctttggagct aaaggagtta
     2401 cccttttaga tagtgataat gatggaagac caaatgctta tgatattgat agtgataatg
     2461 atggtattac tgataatacg gaagcacaag caacttgttc cttcaaaatg cctttatact
     2521 cagattgcga taatgatggt gtagatgatg cttatgatat aggtggctgt gcatcttgta
     2581 caagaactag tggtggatta actcctttcg ataaagatgg agattctaca cctgactatt
     2641 tagatttaga tactgataat gatagtgcac cagatattta tgaaggccac accatcaaaa
     2701 ctgttaacta ttggattggt gcaacaggcg atgcagacaa agatggttta atggattatt
     2761 ttgatgggta taacattatt acagcaacag atggtttata ttggagaaat gtaatcaata
     2821 ataatatggg caactttggt tcttgggata caggtgttgg atctactggt agtatttctc
     2881 aattaccaaa aagcagacca ggagcttgta atgtaggtga tagagattgg agagatttct
     2941 atattcttcc tgtaactatt gttgaattta aaggaaatat aacaagtgat gctattgcta
     3001 aattaaactg gagtgttaca aacgaaataa atattaacca ttatgattta gaaagaagta
     3061 caaatggttc tgaatttagt aaagtagcac aggtaaatgc aatcaatgct tcttcttcat
     3121 ctgcaatcag taattacaat gcaaatgata atgttgcagg attaaatgga attatttatt
     3181 atagattaaa aattgtagaa aatgatggta ctattaagta tagtaatgta atcaatttca
     3241 aattaagcca atctaccaga actgtggttt cattatatcc aaacccagca gttaataact
     3301 ttactgttaa aataacatcg ccaaaagatg aaacagtgca ggtacgtgta tttgatatgt
     3361 taggaaaagt gttattaagc caacaatcta ctattgttgt aggtatcaat aatttatctt
     3421 tcaataatat aagcaaatta tctacaggca cctatactgt acaaataatt atgagcaacc
     3481 aagtgtttac tgaaaaattg gttgttacaa aataattaat ccctatgttt attctaaagc
     3541 tgcttttatt aaaagcagcc ttttttattc agtaatttag ctgcattaat tggcattata
     3601 ttttgggtat aaattaaatt aactaaagtg tagtttagtt tatttttaca taaactcttg
     3661 cttcgtaagc ttgtaattca aaattagatg tatagttaaa tgagaattca aaatcagaaa
     3721 ataagttttt aaaattgcca gaaagccatt catgctctag cataaatttt actttatttt
     3781 cttgagataa gttaagcaaa actattacta tattttcctc atattttctt atataaacta
     3841 aaatgttgtt gttgcttatt gaaataaaat ttgtttgtcc ctgagatatt gctttatttt
     3901 ttctcaagtt taaaagcttt gtataaaatt gttctaataa aaaagtttta ttccattgta
     3961 ttgtatcttt ttcaaaaaat gccaatcgtt ttaggttagg attttcttgc ccactataca
     4021 gtaaggaaat acctttaaag gtacaggata gaactgcaaa agctttagca gcatctccat
     4081 acttttcgta ttcagttcca ttccaactat tctcatcatg attgctggtg aagagtaatt
     4141 tataagtatt ggttggtaaa ccattatatg tagaaagcaa atcttttatt tggtttaatg
     4201 tgcttttttg tttacaataa ttttcagaga tatgcattaa ttgccatgcg taattagcat
     4261 caaacacgtt taaatattca tcttcatcac attcggctaa ccaaaataat tctttaatta
     4321 catcgcaatg ttttcttgct tcataccaaa aacttaaagg aactaaatgt gccatatcac
     4381 atctaaaacc atcaatatca aattcgttaa tccaatactg cattgcattt atcattgcag
     4441 aacgcatatt gatattggta taatctaaat caattacatc tatccaacca tttttttctg
     4501 taaaatttcc atgttcatct ttaataaacc aatcagagtt tgtaattgcc cattcatggt
     4561 taaaccctgt atgattggca acccaatcaa taattacctt taagccaaga gcatgagctt
     4621 gatttaccag ttctttaaaa tcttctgctg taccaaattc atcattaact tttgtatagg
     4681 cactacaagc ataatagctg cctaagctac cttgcctttt ttctttacta ataggggtta
     4741 agggcataaa ccataaaatg gttataccca tttcttttaa tcgtagtaaa ttattttgaa
     4801 aacttttaat agttccttcc gtagtatatt gtctaatatt tacttcataa atacttgcac
     4861 caattgccca acttacaata ggaaaatgta aagccatatc aataaaatat tgctttaaat
     4921 ataatttaat ttattagttt atgagtagaa ataaaggttg aatttatttg gaagatttag
     4981 gcaagccatg tattacatat tgtaatataa aacttagtag cataaccaat aaagcaccaa
     5041 ccacatttag ccataaaaat cctaagccaa ttataccatt tttatctaaa acaaataagc
     5101 caattatagc aatttcgcta ataatagctg caacaaaaac tgcattacct ttcacttttt
     5161 taatataaaa gcctactaaa aaaataccta aaattactcc ataaaataaa gagcctaata
     5221 catttactgc ttcaattaaa ctacccatac caacaactgt ttctgcaata actatacaaa
     5281 atatacccca gagtaaagta taaattttag agtaacgata atctttaatt gcctgttccg
     5341 tttcatctat catttttttc ttactaaacc ttttatgaaa atctattata gtacaagctg
     5401 caagagagtt taaggctgct gcaatactgc cccatgcagc taaaaaaatg atggcaataa
     5461 ttaatccaac taaaccaaca ggtaaatagt caacaacaaa tcttaagaag atgtagttag
     5521 tatcattttt atctccttca atatttgcag tattaaaaag gtttatgtaa gtatttttta
     5581 tacgtgatgc tgaatcatta atgtgatgaa ttgtttttat tgtttgctct ttattggagc
     5641 tataattttt taatgtttcg gttttaagtg tatttaattt atcaaattca ttttgcagca
     5701 aaatggcact atctttttta ttgctttgta ataatttttg ttcttgaaca ggattgaaaa
     5761 aaattggttg aggcttaaat tggtagaatg caaataacaa aacaccaata agtaaaataa
     5821 aaaattgcat tggtacttta actaaaccat tcattaataa acctaatctg ctttcggttg
     5881 tgttttttgc tgttaaatat cttccaactt ggctttgatc ggttccaaaa taacttaatg
     5941 ctaaaaagaa gccaccaata actccactaa taatattgta cttatccttc cagttataat
     6001 ttccattttc aaaaccagat gttatgatat tcattttatt actaccacca ctaatattaa
     6061 gagcatcaat aaaaccaata ttttttggca acaaatttat aattgtataa cctgcaaaaa
     6121 acatggctgc aaaaatgatg ataagttgaa tttgttgtgt gtaggcaaca gctttagctc
     6181 caccactaac tgtataaatt attaataaac cacccataat aatattggta ataaaaatat
     6241 cccaacccat taaggaagat aaaataatgg aaggtgcata aatgctaatg cctgtactta
     6301 accctctgga taataaaaaa agaaaggatg taaaggctct tgtttttaca tcaaatcttt
     6361 gttctaaaaa ttcgtaagca gtaaaaattt ttaacttacc aaataaagga acaaacgtaa
     6421 tacacaacgc caccattgct aatggtaaac caaaataata ttgtacaaac cgcataccat
     6481 ctgtaaaagc ctgtcctggt gcagataaaa aagtaattgc actagcctgt gtacccatta
     6541 tacttaaaag aattaagtac caaggcatat ttctattgct taaaaaatat ccttctaagt
     6601 tttttgatgt tctgctttta tacactccat aggcaataat agttgctagg gtaacaatta
     6661 gtactatcca gtctatactg ctcttcattt atttgtaagc attaaaatgg ttggtgatac
     6721 aataaaaaat aattattata actaataaaa ctaaagtaat aaaagcatac cagtggctcc
     6781 actttttaaa taaaggaatt ttaggtgtac tcatttttaa taatattaat gcttttctaa
     6841 ttctagcata ttagccaata atttataact tcctgcaatg ccagcaggca actgcctaaa
     6901 caatgctaag ctacaataaa cataatttcc tttgccatat tttgcaatta ataagctact
     6961 gtttgtttct ttttctcctg tatcattcat tcctaaaata cattcaaaat ttgaatcaaa
     7021 cttttctgcc tgataagtac ttctttcttg tacccagttc tcaaaatctg cagaagaaat
     7081 tttattagga taatttaaaa ctgtatggtt gggtaacaaa aatttcacct tagcatcttc
     7141 ttctgtaatt cttgaacgac tatatactgt aaaactgtaa ggaccagatt gaatagcttt
     7201 agtaccaatt gaattagact tgatatattg aacaattaag ttgccaccat tttttacaaa
     7261 tgcattaagt acattatttt tatctgttaa atactcatga atattatatg ctctaatacc
     7321 tattactatt gcattatatt gcttaagcgt ttcaatattt aaatcttttt ctgttaactg
     7381 tgtaactata tatcccattt gttctattgc ttctgctact ttatctcctg caccatttat
     7441 atacccaact tttaaaggct ttgtaataat tttttcgtta ataaactttg tttcgtttgt
     7501 aaaaaaatag tgcaaattgg gaatatgttc gtaactaata ttttttaaat cctgtgtata
     7561 ttgttgctct tttgtctgat agtttatttg aacagatgga gtaataactt cattgaaagt
     7621 ataatttttt aaattggata tggcaatatt gaatgcgtaa gattctgcat tttttaaatc
     7681 taaaatggta tcttttgcat acacaatatt atttgcttga tgtaatgaaa tagtaacaga
     7741 agtttttaaa gcattaatgt ttgaggtaac ttgtaattga atatttttac tatttactat
     7801 ttttttattt tgttgaatat gtagtaaaat attattaggt gtaagattaa ctgttattgg
     7861 tggaacaata attaatggtt cataaatttc gcctcttacc ggatgaacca ttttgaattg
     7921 aataggtaca tttaaagtaa aatcagtacc atcaatattt aaaaaaaatt ttgcaatata
     7981 agttgggctg ttttctgctt ctcccaacat tgcttgattg cttggatgaa acatattttt
     8041 tagaataggc ttagtaagcc aatagggttg agttacaggt tgggttaatg gaataataca
     8101 ttctttagaa aaagagtagt tgctattttt taagagctgt aaattaatta ctgaatcaaa
     8161 agtgttgtta ttgcttattg aaaaagaaat tttacttaat ttaatattag aatcggatct
     8221 gttattactg aaaaaattaa aggtagcttt ttggccttgt actacagctt cttcgctact
     8281 tgttgcttca ataaataaac cactacactt aataattaaa ttttcaattt ctttgatttt
     8341 taaagtaacc caattatttt ttgttggata gttgcttaat aaattataaa tggttactaa
     8401 ttttgaaacg gatgattgtg ggttagcaaa attatattct gctattactt catttatttt
     8461 ttgttgaatt atgcttgttc cttccaatct gctccaagta gtatttaccc tattcataat
     8521 atctgtggta gcactatctc catttacata agcaaagtat tcggtaatat ttccttttct
     8581 ttttggtctg ccttcacctt ggcttttgtg catagttctg gcaatgcctc ctacttcgcc
     8641 ataacttaag cctaacagag ggttgtagtt gccaacttct aactttaatt gattattgtt
     8701 ggttgtatta ttgttgccaa aattaaaagt attccataac aacctttttg cttgccatgg
     8761 ctgtacacca agtgtaaact gttctggaaa ttttgttata tctgctgctg caataaatgc
     8821 ttcttgtgct aaaattgcac tagcagcatg atgtccatga cctgctctgc catcttgtgg
     8881 aaacctattg atgattatat ctggttgaaa ttttcttatg ttccaaacta tttcatataa
     8941 cagttgtaat ctgttccatt tgtttaatgt ttcctctgct gttttgctat aaccaaattc
     9001 ataggcatta gtaaaatatt gttcaccacc atcaattctt cttgcagcta ataattcttg
     9061 agttcttatc attcctaatt ctataccttg ctcatcgcca atcaaatttt ggcctccatc
     9121 tcctctggtt aagcttaaat aagctgttct gtataatttt tctttagcta aatagggtag
     9181 tagtgtatta ttctcatcat ctggatgtgc agctatgtat aaaacacttc ctaaaacatt
     9241 taattttttt agttcaataa atatttcggc actattggtt tgtgctttta agaaagtagt
     9301 aattaataat aatgaactta ctaaaatttt tttcaaaaca ttatctggtt tacaaaatga
     9361 gtaattgggt ataaaaattt attcttgtcc aaccataaat acagcattgc ataacattag
     9421 tttgccgttt tcccaaaaca ttctgaaaat aggattgtcg gcaaaaaata taatatttcc
     9481 tctaccatat tcagttgcac caataacggt accatctttt aaattttctt ttactttata
     9541 gcctgcaaag ccggtaacat aattttcttt ttttaaatat ccaacattcc aaccctcttt
     9601 cataaactca taaatagtag catctaattt taatgtgtaa taaatactat catatccaaa
     9661 tgctaaagga tgtgtgttgt ctaaatctat tttataaata gcaccaggaa tagaagatgg
     9721 taaatagtct ttttctctat cggcatattt tttaagtaaa gtgtaatctg ttttttctgg
     9781 ctctttatct ggattggttt ttaacttaaa ccctacatct aattgtgcta actgatttac
     9841 tgcactttct aatgcaatta atttaccacc agtggataca aattctttaa tcttatcaac
     9901 agtattttta tctgagctaa atttataata gccatctggt aaaataataa cctgatagtt
     9961 tttaaaatta attctgttta attcagtagc gtttactaat gtaattgggt agtttaattg
    10021 cttgtcaaaa aagttccata cttcgcctgc agctaatgag gatacgctct ctcctgtaac
    10081 taataatatt tttttggtat taattaattt tacatgtggg ctaccaaaat ctataccttt
    10141 atccataaaa cctgttgtta caatatcggc atttatgtca aatttattgg cagcaaaatt
    10201 tatttctttg ttgattaaat ttatatcgtt gcctgtttta ataataatta aagtacctct
    10261 ttcataattt tttccattat aaacaaatgg tttttctgca aatcttactt taataccttt
    10321 ttttaataaa tgtgctaaaa cctttaccga attaatactg ttgtattgta taagataacc
    10381 ataattagaa gaataatttc tgattgttgc aggcagcgta acagcataat taatttcttt
    10441 tttttctttt attgcatagg tatttacacc ataagcatac cctaggctcc aagcagtaat
    10501 atcataagtt gcactgtcgt ttaatttact ttttggttct aaaagaatgt tggccaaaat
    10561 accttttggt tggcatgtac ttactgcaat ggtatttgtt aaaggcttaa cttctgcatc
    10621 tttatttaat tggtaacaat atccttttaa ccctttatag tcaatagtgc catataaaat
    10681 tccattttta ttgagtaagt ctttaatagc ctctatatta tatttattat ccg
//
LOCUS       DAOQHP010000026        10487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3177_length_10487_cov_3.593558, whole genome shotgun sequence.
ACCESSION   DAOQHP010000026 DAOQHP010000000
VERSION     DAOQHP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_10487_cov_3.593558"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLY81_01725"
     CDS             complement(<1..365)
                     /locus_tag="PLY81_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28981.1"
                     /translation="MKKLLSLFSICICFTAFIFTSCKKEGFITDSNALLRTSVDTLFY
                     DTVFTSVGSITQSFKIFNPNNKKLKLTSVELMGGNLSFYKMNVDGFSSSIVENIEINA
                     NDSIYVFAMVNINPTVTNLP"
     gene            complement(482..592)
                     /locus_tag="PLY81_01730"
     CDS             complement(482..592)
                     /locus_tag="PLY81_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNF28982.1"
                     /translation="MGRGDKKTAKGKRFKGSFGKSRPHKTKTNKAATKTA"
     gene            complement(681..950)
                     /locus_tag="PLY81_01735"
     CDS             complement(681..950)
                     /locus_tag="PLY81_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF28983.1"
                     /translation="MNKAELIAQLADDAGITKTQANAALDSFVDTVTKTLKKGDKVTL
                     VGFGTFSVSKRAARTGRNPQTGETIKIKAKKVARFKAGKELSSKI"
     gene            complement(1251..1556)
                     /locus_tag="PLY81_01740"
     CDS             complement(1251..1556)
                     /locus_tag="PLY81_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNF28984.1"
                     /translation="MFIYNVTIQVEPSIHDAWLIWLQNIHIPEVMNTGCFTKYQLVKL
                     LDTKEEENTTYAVQYYLESKADYNRYIELFAPLLREAGLLAWGNKFAAFRTFMQVVN"
     gene            complement(1556..3601)
                     /gene="paaZ"
                     /locus_tag="PLY81_01745"
     CDS             complement(1556..3601)
                     /gene="paaZ"
                     /locus_tag="PLY81_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HNF28985.1"
                     /translation="MQKLYNYILGKKITGDGDGQVLYNAVSGNAIATASTAGLNFDDI
                     LSYARNKGSALRKMSFHERGLMLRALANHLRKHLDKFYSISYQTGATKADSWIDIEGG
                     IGNLFANASLRRKLPDTPFCLDGEHHALGKQNTFMGHHLLVPKEGVAIHINAFNFPVW
                     GMLEKIAVNLLAGMPCVVKPATVTSYLTEAVVKEIMDSNILPNGALQLICGSAGNMLD
                     YVTSQDVVTFTGSATTGLMLKSHKRILEENVPFNMEADSLNCIILGNDVTPEMPEWDI
                     FIKEVRKEITVKCGQKCTAVRRIFVPENKIEAVQTALIKALSQTTIGNPLNEKVRMGS
                     LAGNTQRNEVKTQVQKLLASSQLIYGSLDSVETIDADANKGAFISPMLLLNTTPFLTN
                     EVHEVEAFGPVSTLMPYKNLEEAIELSKMGKGSLVSSIVTASEKNATNYVLGAGAYHG
                     RILVLNNECAKENTGHGSPLPLLVHGGPGRAGGGEEMGGLRGIKHYMQRVAIQGSPTM
                     LTAVSKVFQPNAKGNIDGRHPFTKYFEELQIGDQLITEKRLITSEDINKFADLSGDHF
                     YAHKTETDFSGTMFEQQVAHGYFIMSIAAGLFVDGFKKNPVLLNYGIDELRFTKPVYP
                     GAEIYIRFTCKEKIAQDTKDENDLPKGIVKWLVEIFDESNEITGIATILTMVAKKNK"
     gene            3764..4858
                     /locus_tag="PLY81_01750"
     CDS             3764..4858
                     /locus_tag="PLY81_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HNF28986.1"
                     /translation="MKLLFSFLFFSISIVSAQHNYLTFNRDSTVINLTIVGASHLASL
                     PLKCIDIEFPNKTSHTSETLEDHILSPKQLHPAFYGCFDWHSCVHGHWMLLKLLKSFP
                     DLPEAGAIRKVFNKNITLQNIATEVNYFNATLSKTWERTYGWAWLLKLQAELINFNDS
                     DAQKWSKTLQPLTDSIISRWLQFLPKQTYANRTGVHANTAFGLILALDYARKAKHKQL
                     EEAIIKSAKHLFLKDKNAPALWEPNGSDFLSPSLIEADLMSKILTPNNFIIWFNQFLP
                     QKSLAHLLQLPIVSDRSDYQIVHLDGLCFSRVWCMKSIAKLLPENDKRKKLLQKAAKQ
                     HLITSLPNIANGYYGGEHWLASFAVYALME"
     gene            4933..5394
                     /locus_tag="PLY81_01755"
     CDS             4933..5394
                     /locus_tag="PLY81_01755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF28987.1"
                     /translation="MQFKAKQIGIVNVQFGENVTLVEPCNIYGCTIGNNSFVGPFTEI
                     QKDVIIGSNCKIQSHSFICELVTIGNNCFIGHGVMFINDTFSQGKPAGGIKELWKETK
                     IGNNVSIGSNATILPITICDNVVIGAGAVVTKDILKPGVYVGNPAKCIKNL"
     gene            5735..6490
                     /locus_tag="PLY81_01760"
     CDS             5735..6490
                     /locus_tag="PLY81_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNF28988.1"
                     /translation="MKKIVLFGFIIGLFFTSCTNYKKLEKQYQLFQTGLDSMSKYSYK
                     SLTLKPYDVIIIKTYTTATSNQDQTSLFNILGNKFTINQNGTIDLPKIGILKVEGLTC
                     KQLKTVLINEWSKYVKDILVDVQFAGLTVNVLGEVASQGTKKFEKEEVTIIDVLAASG
                     GLNLDAKRNDILVIREDSGRRQHYLLDLRDANFYTSPAYQLQQNDLVYVGASTKKFKN
                     LSGAEFRNNIMPLTQTISIVISFLNFFLLLSNK"
     gene            6514..9006
                     /locus_tag="PLY81_01765"
     CDS             6514..9006
                     /locus_tag="PLY81_01765"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNF28989.1"
                     /translation="MSSNLSPSPISFLSQNNNENKSKFSVRETLMKYLSYFPLFVLSM
                     VLCVGAALMYLRYKVPIYKATLQVMVQQNASSKSSSPQPDLISQAVSGVRAINLENEI
                     QLIQSKALLEKVVIAGNFNIVCSKEGNIKKSDAYKSTPFDFIPLHIPDSSKIFTVKLI
                     AYNTSGGTVITPSGNKNFKWGDSLNIYGLGFRLINNTNNFEAEQSPYIIKWVPVGFMA
                     SQIQGSLSVGTLSQKTSVLLLGLTGESRIKAIDFLNLYVKEIIKKDIELKKEASLATI
                     EFINNRLKIVEKELSDLEIQIRDYKKANRFFDASGEYNYIQIRLSKAEEEVLKLRLDI
                     QNIDYIDNYLRNDKSLGKSKVIPSNLGVEDMSYNTLIAKYNDLQAQKEKEDFVSNKDN
                     PKLIELDNQISQFKQSILEAGAILKQSKLLKISTYDGRTDVDMEKLATLPDKELKMIE
                     INRQKAIKEKLYLYLLQKGEETAIASVSTESNYQAMDDAIASSVPVEPKSTQIKVFSI
                     ILGLLIPIGIIYLLDLLNDKITTRQDIAERTKIPITGEISHLDAVGKIVVENSRNVVA
                     EQFRILRSNLQFILPTANDGKATTILVTSSISGEGKSFISLNLAAVLALTEKKVALLE
                     FDLRKLKGINLDEIEDNNDRGITNFLIGQTNDINSICKTLKKHPNLHLYNTGPIPPNP
                     AELIINSRMEMFFNILKDKYDYIVLDSAPVGLVSDSFALSKYADVDLYVIRQRYTLKK
                     QLDFINDLHNEEKLKNMAIVVNDVNLAGRYGYYGYGYGYGYGYMYRYGMGYGYNRYIY
                     GGRKKDPYFDSNKQGYFDDNVKLKWWQKIFRK"
     gene            9107..10144
                     /locus_tag="PLY81_01770"
     CDS             9107..10144
                     /locus_tag="PLY81_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF28990.1"
                     /translation="MNENQQIIINFLKNNPHTSSKEIMTNAGVIASYATIKRLLQSMA
                     TEGLLEIIGKGKSTKYAVSPVYELIEPISVDTYFKNEIDERKIINSFNYNIFKNLLSA
                     KNIFSNSELGILEKFHVTFKNRIAKLSKNELKKDLERLAIDLSWKSSQIEGNTYSLLE
                     TERLLKEKETAEGKTQEEAFMLLNHKEAIDFIIQHPDYFFTLTISNIEDIHKVLVQQL
                     DIDINVRNKKVGISGTNYKPLDNQFQIKEAMTSMCEIVNQKENVFEKALLTLILLSYI
                     QPFMDGNKRTSRIVSNAILLSYGYCPLSYRTVNSIDYKKAMLLFYEQNNLSAFKNIFI
                     GQYQFAVENYF"
     gene            10222..>10487
                     /locus_tag="PLY81_01775"
     CDS             10222..>10487
                     /locus_tag="PLY81_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF28991.1"
                     /translation="MQHWTEEITPKNSLLDLKLKEVWHYRDLMFMFVKRDFVTFYKQT
                     ILGPLWFFVQPLLTTIMYVIVFGNIAKISTDGLPPILFYLCGIT"
BASE COUNT         3750 a         1525 c         1595 g         3617 t
ORIGIN      
        1 ggtaaattgg ttactgtcgg atttatgttt accatagcaa aaacataaat actatcgttg
       61 gcatttatct ctatgttttc tacaattgat gaagagaaac catctacatt cattttataa
      121 aaagaaagat taccccccat caactctaca gaggttagtt ttaatttttt attgtttgga
      181 ttaaaaattt taaacgattg tgtgatggat ccaacagaag taaaaacagt atcataaaat
      241 aaagtatcta ccgatgttct gagtaatgca ttgctatctg taataaatcc ttctttttta
      301 caacttgtaa aaataaaagc agtaaaacat atacaaatac taaaaaggga taacaatttt
      361 ttcatataaa atcaagaaac atcgtttaaa tatacaatca attaaataaa aataaaaagg
      421 ttatgggtta atacaaaaaa gcctgcaact tttgcaggca ataattttgt tcaattcttt
      481 attaagcagt tttagttgct gctttgttgg ttttagtttt gtgcggtctg cttttaccaa
      541 atgaaccttt aaaacgtttg cctttagctg tttttttatc acctctaccc atgattgtgt
      601 ttttagttat tttaatgaat tgcagcaaaa ataaaaaatc ctgctgaaat gagcaggatt
      661 ttttattaaa gttcttaaaa ttagattttt gaacttaatt ctttaccagc tttaaaacga
      721 gctacttttt tagctttaat tttaattgtt tcgccagttt gaggatttct tcctgtacga
      781 gctgcacgtt tagaaacaga aaaagtacca aaacctacta aagttacttt atctcctttt
      841 tttaatgttt tagtaactgt atcaacaaaa gagtctaaag cagcattagc ttgagttttt
      901 gtgatacctg catcgtctgc aagttgtgca atcaattcag ctttgttcat gattaattgt
      961 gtttttaatt gtttaattag ttagtgaaaa caaatttatt agcttttgca atgcaacaaa
     1021 atatttttat ttgtttttgt tttttttata ttctttaaaa cccttactaa taaaggattt
     1081 tagaagaatt atttaaaatg atgcataaaa taaaacaacc ttaatcggct gaaaccattt
     1141 atggcaaagg gttttagctg atattgctca agtgcagtaa aatcaatagt tgtagaggaa
     1201 tataagaaag aaattgaata aacaaaattg ttttataaat ctatccacaa tcaattcaca
     1261 acctgcataa atgttctaaa tgcagcaaat ttattacccc aagccaataa accagcctct
     1321 cttaataaag gtgcaaacaa ctcaatatac ctattataat cggctttact ctctaaataa
     1381 tattgaacgg cataggttgt attttcttct tccttagtgt ctaacagttt tactaattgg
     1441 tattttgtaa aacaacctgt attcattact tcgggtatat gaatattttg tagccatatt
     1501 aaccatgcat catgtatgct tggttctact tgaattgtta cgttataaat aaacattatt
     1561 tatttttttt agcaaccatg gttagtatag ttgcaatacc tgttatttca ttgctttcat
     1621 caaaaatttc aaccaaccat tttacaattc ctttaggtaa atcattttca tctttggtat
     1681 cttgtgcaat tttttcttta caggtaaatc gtatgtaaat ttcggcaccc ggataaactg
     1741 gtttggtaaa tcttaactca tcaattccgt agttcaacaa taccggattt tttttaaagc
     1801 catctacaaa caaacctgct gcaatactca taataaaata tccatgagct acttgttgtt
     1861 caaacattgt tcctgaaaag tctgtttctg ttttatgggc ataaaaatga tcaccactaa
     1921 ggtctgcaaa tttgttgatg tcttcactcg ttatgagcct tttttctgtt attaactgat
     1981 ctccaatttg tagttcttca aaatatttag taaaaggatg acggccatca atatttcctt
     2041 ttgcatttgg ctgaaatact ttagaaactg cagttaacat tgttgggcta ccttgtatgg
     2101 caactctttg catataatgt tttattcctc ttaagcctcc catttcttct ccacctcctg
     2161 cacgtcctgg tccaccatgt actaataaag gtaatggact accatgccct gtattttcct
     2221 ttgcacattc attatttaaa actaaaattc ttccatgata agcacctgca cctaaaacat
     2281 aatttgttgc atttttttca gaagctgtaa caatagaact tacaagagag cctttcccca
     2341 tttttgaaag ctcaatggct tcttctaaat ttttatatgg cattaatgta ctcactggcc
     2401 caaaagcttc tacttcatgc acttcgttag ttaaaaatgg tgtagtattt aataaaagca
     2461 ttgggctaat aaatgcacct ttatttgcat cggcatcaat tgtttctaca ctatctaaac
     2521 taccataaat taattgtgag gaagccaaca gtttttgaac ttgtgttttt acttcattac
     2581 gttgtgtatt acctgccaaa cttcccattc taactttttc attcaagggg ttgccaatgg
     2641 tagtttgaga aagtgcttta attaatgcag tttgtactgc ttctatttta ttttcgggta
     2701 caaaaattct tcttacagct gtacattttt gtccacattt tacggttatt tcttttctaa
     2761 cttctttaat aaaaatatcc cattctggca tttcaggtgt tacatcgttg cctaaaataa
     2821 tgcagtttaa cgaatctgct tccatattaa atggcacatt ttcttctaaa attcttttat
     2881 gagatttaag cattaagcct gtagtggcac tacctgtaaa agttactaca tcttggctag
     2941 ttacataatc taacatatta cctgcactgc cacaaataag ctgtaaagca ccgtttggta
     3001 aaatgttaga atccattatt tctttaacca ctgcttctgt taaataggaa gtaacggtag
     3061 ctggttttac cacgcatggc attcctgcca ataaattaac agctattttt tctagcatac
     3121 cccaaacagg aaagttaaag gcattgatat gtatagctac cccttcttta ggaactagaa
     3181 ggtgatgacc cataaaagta ttttgtttac ctaaagcatg atgttctcca tctaaacaaa
     3241 aaggagtatc gggtaatttt cttcttaaag atgcgttagc aaataggtta ccaataccac
     3301 cttcaatatc aatccaacta tctgcttttg tggcacctgt ttggtagcta atagaataaa
     3361 atttatctaa atgttttctt aaatggttgg ctaaggctct taacattaaa cctctttcat
     3421 gaaaactcat tttgcgtaaa gcactacctt tatttctggc ataacttaaa atatcatcaa
     3481 agtttaaacc tgctgtactt gctgttgcta ttgcatttcc tgaaactgca ttatataaaa
     3541 cttgcccatc accatctcct gtaatttttt ttcctaaaat ataattgtaa agcttttgca
     3601 taaatatttt ttatgagtaa aatacaaatc taaggtaaac tagttttatg ttttgaatat
     3661 acctgctatg aataattttt atataattta aataagagta ggaattaaaa ttcaattatc
     3721 accattatca atttgtactt atttttatag taattttaaa agtatgaaat tgcttttttc
     3781 tttcttgttt ttttcaataa gtattgtatc tgctcaacac aattatctta catttaatag
     3841 agattctact gtaattaact taacaattgt tggagcaagc catctagcat ctcttcctct
     3901 aaaatgtata gacattgagt ttcctaataa aacaagccat acgtcagaaa ctttagaaga
     3961 tcatattctt tctcccaaac agttgcaccc tgcattttat ggatgctttg attggcatag
     4021 ctgtgtacat gggcattgga tgttattgaa attattaaaa agttttccag atttaccaga
     4081 agctggtgca ataagaaagg ttttcaacaa gaatattact ttacaaaata tagcaaccga
     4141 agtaaattat tttaatgcaa cattatctaa aacatgggaa agaacttatg gttgggcatg
     4201 gttattaaaa ctgcaagcag agctaataaa ttttaatgat tctgatgctc aaaaatggag
     4261 taaaacttta caaccattaa cagattctat aattagcaga tggcttcaat ttttacccaa
     4321 acaaacttat gcaaatcgca ctggtgtaca tgccaatact gcatttggcc taattttagc
     4381 acttgattat gctagaaagg caaagcataa gcaattggaa gaagcaatta ttaaatcggc
     4441 aaagcattta tttttaaaag ataaaaatgc accggcattg tgggagccaa atggaagcga
     4501 ttttttatcc ccttcattaa ttgaggcaga tttaatgagc aaaattttaa cccctaataa
     4561 ttttataatc tggtttaacc aattcttacc acaaaaatct ttagcacatt tgctgcaatt
     4621 acccattgta agtgatagaa gtgattatca aattgtacat ctagatggct tgtgttttag
     4681 cagagtatgg tgtatgaaaa gcattgcaaa acttttaccc gaaaatgata aaagaaaaaa
     4741 gcttcttcaa aaagcagcca agcaacattt aataactagc ttgcccaata ttgccaatgg
     4801 ttattatgga ggcgaacatt ggcttgcaag ctttgcagtg tatgcactaa tggaatagtt
     4861 tttcagtatt caaaacttaa ttcaaataat aatattggag gttgtaaatt gcaaaccaaa
     4921 aattaatttg ttatgcagtt caaagccaag caaataggca ttgtaaacgt acaatttgga
     4981 gaaaatgtta ctttagtaga gccttgtaat atttatggtt gtactatagg taataacagt
     5041 tttgtaggac ctttcactga aatacaaaaa gatgttatta ttggtagtaa ttgtaaaatt
     5101 caatcgcata gttttatttg cgaattggtt actattggta ataattgttt tataggccat
     5161 ggcgttatgt ttattaacga tacttttagt caaggtaaac ctgcaggagg aataaaagaa
     5221 ttatggaaag aaacgaaaat tggaaataat gtatctatag gtagtaatgc aacaattttg
     5281 cccataacaa tatgcgataa tgtagtaata ggtgctggtg ctgtagttac taaagatatt
     5341 ttgaagcctg gtgtatatgt tggtaaccct gctaaatgta ttaaaaattt gtaacagaat
     5401 tagttgcgta ttttttttaa aataataatg ggaatatatt aggacaaaaa agcaaatgaa
     5461 aaaaaatatt ttctgactta aatacctata tttgcgactc cctaaaattg agtaatgaaa
     5521 cagtgtagct aaacctagtt tagttttagt gtttatgaaa gaactaaaaa ttaagtattt
     5581 gatataaatt tataataggc atacgggtaa aatttaattt ttaattacct gttatgccta
     5641 tgttttttag ggtatttact gacagcaatg tgagtcaggt ggcggagctg tacatttttt
     5701 aaattaactc ttatttttta aactattagt ggatatgaag aagattgttt tatttggttt
     5761 tattatcggc ctgtttttta cttcctgtac taattataaa aagctagaaa aacaatacca
     5821 gttatttcaa acagggttag atagtatgag taaatactcc tacaaaagtt taaccttaaa
     5881 gccttatgat gttattatta ttaagactta tacaacagca acttctaatc aagatcaaac
     5941 aagcttattt aatatactag gaaataaatt tacaatcaat caaaatggta caattgattt
     6001 acctaaaatt gggatactta aggtagaagg cttgacttgt aaacaactaa aaacagtttt
     6061 aattaacgag tggagcaagt atgtaaaaga tattttggta gatgtgcaat ttgctggttt
     6121 aactgtaaat gtgttaggtg aggtagcctc tcagggaaca aaaaaatttg aaaaagaaga
     6181 agtaaccatt attgatgttt tggctgcttc tggaggtttg aatttagatg ccaaaagaaa
     6241 tgatatttta gtaataagag aagatagtgg gagaaggcag cactatttat tagacttaag
     6301 agatgcaaat ttctatactt caccagcata ccaattacaa cagaacgatt tggtttatgt
     6361 gggtgctagt accaaaaagt ttaaaaattt aagtggggca gagtttagaa ataatattat
     6421 gcctttaaca caaactatta gtatagtaat aagcttttta aactttttct tattactttc
     6481 aaacaagtaa aaaatcattc aatataagag ttaatgagtt ctaatctttc accatctcct
     6541 atttcttttt tatctcaaaa taataacgaa aacaaatcaa agttttcagt tagagagact
     6601 ttaatgaagt acttaagtta cttcccatta tttgtacttt ctatggtttt atgtgtaggt
     6661 gcagcattaa tgtacttaag gtacaaagta ccaatataca aagccacttt gcaagtaatg
     6721 gtacagcaaa atgcaagtag caaatcatct tctcctcaac ccgatttaat aagccaggct
     6781 gtatcaggtg taagagctat taatcttgaa aatgaaatac agctaataca atcaaaagct
     6841 ttattagaaa aagtggttat tgctggtaat tttaatattg tgtgtagcaa agagggtaat
     6901 ataaaaaaat cagatgctta taaatctact ccatttgact ttattccttt acatattcca
     6961 gactcatcta aaattttcac tgtaaaacta attgcatata atacaagcgg aggaactgtg
     7021 ataactccat ctggaaataa aaactttaaa tggggagatt ccttaaatat ttatggctta
     7081 ggatttaggc taattaacaa tacaaataat tttgaagcgg aacaatctcc ttacattatt
     7141 aaatgggtgc cagttggctt tatggcatct caaattcaag gtagtttatc tgttggtact
     7201 ttaagccaaa aaacaagtgt tttactctta ggattaacag gcgaaagccg aattaaggca
     7261 attgattttt taaatctgta tgtaaaagaa attattaaaa aagatattga acttaaaaaa
     7321 gaagcctcct tagcaactat agaatttatt aataatagat taaaaatagt agaaaaagaa
     7381 ttaagcgacc ttgaaataca aataagagac tataaaaaag caaacagatt ttttgatgca
     7441 tctggcgaat ataattatat acaaataaga ttgtctaaag cggaagaaga agttttgaaa
     7501 cttagactag atattcaaaa tatagattat atcgacaact atttaagaaa cgataaaagc
     7561 ttaggtaaat caaaagtcat tccttctaac ttaggagttg aggatatgtc gtacaataca
     7621 ttaatagcca agtataacga tttacaagca caaaaagaaa aagaagattt tgtatccaat
     7681 aaagataacc ctaaacttat agagttagat aatcaaatta gtcagtttaa acaaagtatt
     7741 ttagaagcag gagctattct aaaacaatca aaattattaa aaataagtac ctatgatgga
     7801 agaacagatg tagatatgga aaagttagct acactgcctg acaaagaatt aaaaatgatt
     7861 gaaataaaca ggcaaaaagc aattaaagaa aaattatatt tatatctctt acagaaagga
     7921 gaggaaacag ccattgcttc cgtatctaca gagtctaact atcaagcaat ggatgatgca
     7981 attgcttcat cagttcctgt agagcctaaa agtactcaaa ttaaagtatt ttccattatt
     8041 ttaggattac tcattcctat tggtattatt tatttattag acttactgaa cgataagatt
     8101 actacaaggc aagatattgc tgaaagaact aaaataccca ttacaggtga gataagccac
     8161 ttagatgcag tgggtaaaat tgtagtagaa aacagtagaa atgtagtagc cgaacagttt
     8221 agaatattaa gaagcaactt gcaatttatt ttgcctacag ccaatgatgg caaagcaaca
     8281 acaatattag ttacctcatc cattagtgga gagggaaaat catttattag cttaaatctt
     8341 gcagcagtat tggctttaac cgaaaaaaaa gtagctcttt tagagtttga cttacgtaaa
     8401 ctaaaaggta taaatttaga tgaaatagag gataataatg atagaggtat taccaatttt
     8461 cttatagggc aaactaatga cattaattct atttgtaaaa ctttaaaaaa acatcctaat
     8521 ttacatttat ataatacagg tcctattcct cctaatcctg ctgagttgat tattaacagt
     8581 agaatggaaa tgttttttaa tattttaaaa gataaatacg attatattgt attagactct
     8641 gcaccagtag gcttagtaag cgattctttt gcattaagta aatatgccga tgttgatttg
     8701 tacgttattc gccagcgata tacattaaaa aaacaattag actttattaa tgacttacat
     8761 aatgaagaga aattaaaaaa tatggccatt gtggtaaacg atgtaaatct tgcaggccga
     8821 tatggctatt atggttatgg ctatggttat ggttatggtt atatgtatag atatggaatg
     8881 ggttatggct acaatagata catttatgga ggaagaaaaa aagatcctta ttttgatagt
     8941 aataagcaag gctattttga tgataatgta aaactaaaat ggtggcaaaa aatatttaga
     9001 aaataattct taaatatttt gagctcaagt tttcctaaaa tatggctcaa ttttacctta
     9061 aaatgaactt taagtaacta ttaattgagc tatataaatt taagttgtga acgaaaatca
     9121 gcagataatt attaattttc tcaagaataa ccctcatact tcttcaaaag aaattatgac
     9181 taatgctgga gttatagcaa gttatgcaac tataaaacgt ttacttcaat caatggcaac
     9241 tgaagggtta ttagaaatta ttggaaaagg gaaaagcaca aagtatgcag tatcgcctgt
     9301 atatgaatta attgaaccta tatccgtgga tacttatttt aaaaatgaaa ttgatgaacg
     9361 aaaaataatt aactctttca attataacat ttttaaaaac ttattatcag ctaaaaatat
     9421 ttttagcaat tcagaattag gtatcttaga aaaatttcat gttactttta aaaatagaat
     9481 agcaaagctt tctaaaaatg aattaaaaaa agatttggaa cgtttagcta tagatttatc
     9541 ttggaaatca tctcaaatag aaggcaatac ctattcttta ttagaaacag aaaggctatt
     9601 aaaagaaaaa gaaacagcag agggcaaaac acaagaagag gcttttatgc ttttaaatca
     9661 taaagaagct attgatttta ttatacagca cccagattat ttttttactt taacaatctc
     9721 taatattgaa gatattcata aagttttagt acagcaatta gatattgata taaatgttag
     9781 aaataaaaaa gtgggtattt ctggtaccaa ttataaacct ttagataatc agtttcaaat
     9841 aaaagaagct atgacttcta tgtgtgaaat tgtaaatcag aaagaaaatg tttttgaaaa
     9901 agcattactt actttaattc ttttatccta catacaaccc tttatggatg gcaataaaag
     9961 aacttcaaga attgttagca atgcaatatt attaagttat ggttattgcc cactttccta
    10021 cagaactgta aattctattg attacaaaaa agctatgtta ttattttacg agcaaaataa
    10081 tttatctgcc tttaaaaata tttttattgg tcaatatcaa tttgcagtag aaaattattt
    10141 ctaaaactaa aagggtaaaa ttttttttaa aaaagataat ttaattaata ccaataaatc
    10201 aagacaaata aaatagaata catgcaacac tggacagaag aaataacacc caaaaactcc
    10261 ttgctagatt taaaactaaa agaagtatgg cattacagag atttaatgtt catgtttgtt
    10321 aaacgagatt ttgtaacttt ttataaacaa actattttag gtccgctttg gttttttgta
    10381 caacccttgc ttacaaccat tatgtatgtt attgtttttg gcaatattgc taaaatatct
    10441 acagatgggc ttcctcctat tttattttat ttgtgtggca ttacatt
//
LOCUS       DAOQHP010000027        10472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3188_length_10472_cov_3.862244, whole genome shotgun sequence.
ACCESSION   DAOQHP010000027 DAOQHP010000000
VERSION     DAOQHP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_10472_cov_3.862244"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLY81_01780"
     CDS             <1..804
                     /locus_tag="PLY81_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF28992.1"
                     /translation="TNLDNLIDGVNKSIADKLNLKNTSILLPKNNMLQKLYTFVPINY
                     AEKVKEALFAVGAGEIGNYSECSFNTKGIGTFNANQLSKPFVGNKGLRHEEVEIKVEI
                     IYPIWLKSKIINTLKNVHPYEEPAFDIISLQNQYNKIGSGLIGELAKPISAKMFLQNL
                     SKVFDLQIIRHSKLNKKNIKKVAICGGAGNFLTTFAMQASADIFITSDIKYHEFFDTN
                     SSMILADIGHFESEQFTIELLQNYLHKKFPNFAVLKTKVKTNPVEYFIV"
     gene            918..1673
                     /locus_tag="PLY81_01785"
     CDS             918..1673
                     /locus_tag="PLY81_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNF28993.1"
                     /translation="MAAVKEFSVEEKLVSLVKLQKIDSKLDEFQILKGELPLEVSDLE
                     DEIQGLHARKNRIEEEVNGVIEFIESKKVAIKEAEDLIKKYDKQSSNVKNNREYEAIN
                     KEMEMQQLEMKLAEKHIKDANEEIADKAKALEIAKKNIATKEGVLNHKKEELEKIIAD
                     TEKEETQFRKKSDSARKDVEERLLYSYDRIRKSYRNGLAVVPVERDACGGCFNSIPPQ
                     KQSEIRLHKKVMVCENCGRILVDTDLNDSVTTK"
     gene            1874..2722
                     /locus_tag="PLY81_01790"
     CDS             1874..2722
                     /locus_tag="PLY81_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF28994.1"
                     /translation="MDEKIREKASLFITIIYLFLGLMLLPIAIGMLTALPEMIGFLGV
                     ALLISSILFFYSAIKQFASNAKQKKANANFIQQINKQNKITNIAKQHDTIVNENIDIA
                     IDCSSSLSYSINNNNSVIANWQLSNEDWIKFYKNEKKERITSIIIEVFWILLLGTILL
                     KNTRMADTLDALLVSGLIGGVYGFGKYFLTMKSISPDVNSTENKEICITNSSILINGK
                     LNIFNDETRWFKSAKLLDNTNPLVVEITFGWKTRRGNTYDEIRIPVNNKETANNIISS
                     LYKKNN"
     gene            2761..3561
                     /gene="pyrF"
                     /locus_tag="PLY81_01795"
     CDS             2761..3561
                     /gene="pyrF"
                     /locus_tag="PLY81_01795"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNF28995.1"
                     /translation="MNKSYLEQQIQQKKSYLCVGLDTDITLIPDHLQQLPNAIVTFNK
                     AIIDATKDYCVSYKINTAFYEALGTKGWEIMEETLHYIPNTHFKIADAKRGDIGNTSA
                     RYAKAFFETLPFDAVTVTPYMGEDSIKPFLEYENKFTIVLGLTSNKGANDFELLKIGE
                     QFLYEKVLEKVSQWGTPNNLMFVVGATQANELQHIRKIVPENFLLIPGIGFQGGSLKD
                     VSTNGFNKNVGILVNVSRAIIYAGTNQQFATDAKEIALEYQTEMSSYL"
     gene            3692..4735
                     /locus_tag="PLY81_01800"
     CDS             3692..4735
                     /locus_tag="PLY81_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNF28996.1"
                     /translation="MALLHNRISNKELKEKLMREVEPRTTISFYCYFHIINPQEFRDF
                     LYKNLSTLKVFGRIYVAKEGINAQISVPTNYFNELKTFLYRIEPLHNLRLNIAVDDDG
                     KSFWVLKIKVRDKIVADGITDDAFDMKNKGKYVNAFGMNKLLEDKNTIVIDMRNHYEY
                     EVGHFENAIEIPSDTFREQLPMAVEMMKGNENKNIVMYCTGGIRCEKASAYMLHKGFQ
                     NVFHLEGGIINYAKQIKEEGEPSKFIGKNFVFDNRLGERITSDVIAKCHQCGATCDEH
                     TNCKNNGCHLLFIQCKNCANKYAGCCSEACKETINMSEERQKELRKGTENGIMIFNKS
                     KARLRPKLNESKP"
     gene            complement(4742..5830)
                     /locus_tag="PLY81_01805"
     CDS             complement(4742..5830)
                     /locus_tag="PLY81_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNF28997.1"
                     /translation="MRRFTFNLIYTSLAILIFSCNKNESTVNPIPTSVSISLDKTSVT
                     ADGIDEIKVTVKDQDGNDISASSVIFVDGFAIKGSAVFFEANQQGTHKVYANKYNVIS
                     DTLTVTTNAPTAAKYSTKVLAEFYTATWCGWCPRMDYKLNNFMAANNNLIAVQIHSND
                     NLANRTVDSIMRNDYGVSSVPNVLINRTSFMQENGDVNNLNDSTEFKKFYQKRAVVGL
                     AINSTVNGNNLDITAKTGFDVTIKDSLKLVILVVENNKIAPQANYYNNNLSYPGNPFF
                     SAGDTINTFVHNGVYSLSPTSIKGITVPVANQTKDNEYIANYTVDINGLNSANLKVIA
                     FVYFANDQARKGILNVQWANAGQNKNYD"
     gene            complement(5989..7308)
                     /locus_tag="PLY81_01810"
     CDS             complement(5989..7308)
                     /locus_tag="PLY81_01810"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNF28998.1"
                     /translation="MKVMKFGGTSVGKPERMHQVATLISQNNQPKIIVLSALSGTTNS
                     LIEICNLLEQGNRETAKQKIDELEIHYKNFIIDLLSKEEYQAKAYEFISEHFDFLNII
                     LKISFSEALSKDILAQGELLSTKLFSTYLSEVGIQHTLLPALEFMTIDNNEEPQIGSI
                     KVKLTQLLQQHTNCTIFVTQGYICRNAKGEIDNLKRGGSDYTASLIAAAINASVCEIW
                     TDIDGLHNNDPRIVDKTYAIEQLSFDEAAELAYFGAKILHPACIWPAQHFNIPVKLLN
                     TMQPSAFGTLINDNKPTKGVKAVAAKDGIIAIKVKSSRMLLAYGFLKRIFEVFEKYKT
                     SIDMVTTSEVAVSVTIDNNTHLKKIILELSEFSSVELVFEQTIISIVGNEINAQNEIM
                     PKIFSAISQIPVTMVSYGGSGHNISLLVPRNLKNQTLKALNTGLFDL"
     gene            complement(7490..8677)
                     /locus_tag="PLY81_01815"
     CDS             complement(7490..8677)
                     /locus_tag="PLY81_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNF28999.1"
                     /translation="MQLKEKITLLAKQYSNEFIELRHHIHANPELSYEEFETCQFIQQ
                     KLTSFGIVSSTLATTGVVALIEGNNPSKRVIALRADMDALPIMEENVVPYKSKKNGIM
                     HACGHDVHTTCLLGAAKILQETKTEWEGTIKLIFQPGEEKNPGGASIMIKEGVLNNPK
                     PDGIIGMHVHPGLPIGKFSFRKGRVMASADEIYITINGNGGHAAAPHLTTDTVLIAST
                     IIVSLQQIISRNNNPTIPSVLSFCAINGGTATNIIPSEVKLMGTLRAMDETWRYKAHQ
                     LITKHATAIAESMGATINIKIDIGYPTVDNDEALTTTAWQLADDFWGKEHVEETELRM
                     GAEDFGYYSQQIPGCFFRLGVRNELKGIIHNVHTPKFNIDEQAIENGIAMMAWLGATT
                     AVK"
     gene            complement(8689..9111)
                     /locus_tag="PLY81_01820"
     CDS             complement(8689..9111)
                     /locus_tag="PLY81_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF29000.1"
                     /translation="MKVFWLFILLFCCGQFLYASDTIIVHKDSRLDILTQKQIIINKR
                     SSMLTSTGQYKGFRLQIISTREREKAFNIKAELLTKYPEEKSYVLFQSPYFRVRIGNF
                     VKREDAEKFRKILNKTYKNGVFIVEDVIDYIPPPDEEF"
     gene            complement(9126..10010)
                     /locus_tag="PLY81_01825"
     CDS             complement(9126..10010)
                     /locus_tag="PLY81_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF29001.1"
                     /translation="MNRLQVLKTYKIYIGGQFPRTESGRYYTPLNKTGKPLANICLSS
                     RKDFREAVIAARNAFGGWSSRAAFNRGQILYRIAEMLEGRKEQFINELKQQGSSHNQA
                     ESEVNTAIDRLIYFAGWCDKFQQFSSSVNPVASSHFNFSTPEPTGVVSIIAPQSSSLL
                     GLVSVIAPIIAGGNTCIVLASEQKPLCGITFAEVINSSDVPSGVVNILTGKPSELVKY
                     FADHMDVNAMVYCENDKDSQKLIQDKAALNLKRVLLYNDINWNNSEAENPYLILDAQE
                     IKTTWHPIENIGGAKAGY"
     gene            complement(10042..>10472)
                     /locus_tag="PLY81_01830"
     CDS             complement(10042..>10472)
                     /locus_tag="PLY81_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF29002.1"
                     /translation="SKQQMDTILKYLKIGEKEGAVMHQSQCSIPKKGYFIRPTLFTNV
                     AQSSRIVQEEIFGPVLTIQTFRTDDEVIEKANNTPYGLSAGVWTDKGSKIFNLTTKMR
                     AGVVWANTFNKFDPTSPFGGYKESGFGREGGLHGLGAYLK"
BASE COUNT         3667 a         1533 c         1596 g         3676 t
ORIGIN      
        1 accaatttag ataacttaat agatggcgta aacaaatcaa ttgccgataa attaaattta
       61 aaaaatactt ctattttatt acccaaaaat aatatgctgc aaaaactgta cacttttgtg
      121 ccaataaatt atgccgaaaa agtgaaagaa gctttgtttg cagtaggggc tggcgaaata
      181 ggcaattata gtgagtgtag ttttaacaca aaaggtattg gtactttcaa tgctaatcaa
      241 ctgtctaaac catttgtagg aaataaagga ttaaggcatg aagaagtaga aataaaagta
      301 gaaattattt atcctatttg gcttaaaagt aaaataataa atacattgaa aaatgtacac
      361 ccttacgaag agcctgcttt tgatattatt agtttacaaa accaatataa caaaataggg
      421 agtggtttaa ttggtgaatt agcaaaacca attagtgcta aaatgtttct acaaaattta
      481 tctaaagtat ttgatttaca aataattagg catagtaaat taaataaaaa aaatattaaa
      541 aaagtagcaa tttgtggtgg tgctggcaac tttttaacaa cttttgctat gcaagcaagt
      601 gcagatattt ttataacatc tgatataaaa tatcatgaat tttttgatac aaattcttcc
      661 atgattttgg cagatattgg acattttgaa agtgaacaat ttactataga attattacaa
      721 aattatttac acaaaaaatt ccctaacttt gccgtcctta aaactaaggt taaaacaaac
      781 cctgtagaat actttatagt ttaatacaaa aatgttttag ctactttgtt aataaatagt
      841 acaagtgagt aatgcaataa atgctttaag taattgcaga acacactatc ataaaaatgt
      901 aaactttaaa taagtatatg gcagcagtta aagaattctc tgttgaagaa aaattagtaa
      961 gcttagtaaa gcttcaaaaa attgatagta aattagatga gtttcaaatt ttaaaaggcg
     1021 aattaccatt agaagtaagc gatttagaag acgaaattca aggcttacat gctcgtaaaa
     1081 acagaattga agaagaagtg aatggagtta ttgaatttat tgaaagcaaa aaagtagcaa
     1141 ttaaagaagc agaggattta attaaaaagt atgataagca aagtagcaat gttaaaaata
     1201 acagagagta cgaagcaata aacaaagaaa tggaaatgca gcaattagaa atgaaacttg
     1261 ctgaaaaaca cattaaagat gctaacgaag agattgctga taaagccaaa gcattagaaa
     1321 ttgccaagaa aaatattgca accaaagaag gtgttttgaa ccacaaaaag gaagagctag
     1381 aaaaaattat tgccgatact gaaaaagaag aaacacagtt tagaaaaaaa agcgattctg
     1441 caagaaaaga tgttgaagaa cgtttattat atagctacga tagaattaga aaaagctata
     1501 gaaatggatt agctgtagta cctgttgaac gtgatgcttg tggtggctgt tttaactcaa
     1561 ttcctcctca aaagcaaagt gaaatacgtt tacacaaaaa agtaatggtt tgcgaaaatt
     1621 gtggtagaat attagtagat acagatttga atgatagtgt aacaactaaa taagttttac
     1681 aataattaaa atttacttgg aggctgcctt taaaagtagc ctctattttt ttgtagaaat
     1741 gcaagaaaaa tatgggtact tatatttttt attttttaaa ttagatgggc tttgaatgat
     1801 tcaaaaaata agattagttt tacaaagaca catgccaata aacaagtaag tagtaacaat
     1861 aaaatgattt ataatggatg aaaaaattag agagaaagct tctttattta tcactataat
     1921 ctatttattt ttaggattaa tgctattacc tattgctata ggaatgctta cagcattacc
     1981 agaaatgata ggctttcttg gagttgcatt acttatttca tctattttgt ttttttattc
     2041 tgcaattaag caatttgctt caaatgctaa acagaaaaaa gcaaatgcaa atttcattca
     2101 gcaaattaat aagcaaaata aaataacaaa cattgctaaa caacatgata ccattgttaa
     2161 tgaaaatata gatatagcta tagattgtag ttcatcttta tcatatagca taaacaataa
     2221 taattctgta attgcaaatt ggcaattaag taatgaagat tggataaagt tttataaaaa
     2281 tgaaaagaaa gagaggatta ctagtattat tattgaagtg ttttggatac tgttacttgg
     2341 tacaatatta cttaaaaata ccagaatggc tgatacctta gatgccttgc tagtaagtgg
     2401 attgatagga ggggtttatg gttttggaaa atacttttta acaatgaaaa gtatttctcc
     2461 agatgtaaac tctacagaaa ataaagaaat atgcattact aacagtagta ttttaattaa
     2521 tggtaaatta aatattttta acgatgaaac cagatggttt aaaagtgcaa agcttttaga
     2581 taacaccaac cccttggttg tagaaattac ctttggctgg aaaaccagac gtggtaatac
     2641 ttatgatgaa atcagaattc ctgtaaataa taaagaaact gccaataata taatttcctc
     2701 tctctacaaa aaaaacaatt aattattgaa caactcctaa atctacttta gttttgctgt
     2761 atgaacaaaa gctatttaga acagcaaatt cagcaaaaaa aatcttattt atgtgtaggg
     2821 ttagatactg atattacttt aatacctgac cacttacaac agttacccaa tgcaattgta
     2881 acctttaata aagctattat agatgccaca aaagattatt gtgtaagcta taaaattaat
     2941 acagcatttt acgaagcatt aggtactaaa ggttgggaaa taatggaaga aaccttgcat
     3001 tatattccaa atacgcattt taaaattgca gatgctaaaa gaggcgatat aggtaataca
     3061 tcggctaggt atgcaaaagc tttttttgaa acgcttccat ttgatgctgt tactgtaaca
     3121 ccttacatgg gcgaagacag tattaaacca tttctagagt atgaaaataa atttactatt
     3181 gttttagggc ttacttctaa taaaggagct aatgattttg aattattaaa aataggggag
     3241 cagtttttat acgaaaaagt attagagaaa gtatctcaat ggggtacgcc taataattta
     3301 atgtttgtag taggagcaac acaagcaaac gaacttcaac acatcagaaa aatagttcct
     3361 gaaaattttc ttttaattcc aggtattggt tttcaaggtg gcagtttaaa agatgtaagt
     3421 acaaacggct ttaataaaaa tgttggtata ttagttaatg taagcagggc aataatttat
     3481 gctggaacca accaacaatt tgcaactgat gctaaagaaa ttgcattaga atatcaaaca
     3541 gaaatgagca gttatttata attacgggta taattattta cctttgtact tcaaaatttt
     3601 gcccatcctg caaatgggtt cattgtttaa ctgaaacatt cgttttttac caattaagtt
     3661 gtaatgaaaa tggatgtcta aaattttatt tatggcatta ttgcacaatc gtataagcaa
     3721 taaagagttg aaagaaaagc tgatgagaga agttgaacca cgtacaacta ttagcttcta
     3781 ttgttatttc cacattatta atccacaaga atttagagat tttttgtaca aaaatttatc
     3841 tactttaaaa gtttttggaa gaatatatgt agctaaagaa ggtatcaacg cacaaattag
     3901 tgttccaact aattatttta atgagttaaa aacattttta tatcgtattg agccactcca
     3961 taatttaaga ctaaatattg cagttgatga tgatggtaaa agcttttggg tgttaaaaat
     4021 aaaagtgaga gataaaattg ttgcagatgg tataaccgat gatgcttttg acatgaaaaa
     4081 taaaggcaag tatgtaaatg cttttggaat gaataaattg ttggaagata aaaacactat
     4141 agtaattgat atgcgtaatc attacgaata tgaagttgga cattttgaaa atgcaattga
     4201 aattccaagc gatactttta gagaacaatt gcctatggca gtagaaatga tgaaaggaaa
     4261 cgaaaataaa aatattgtta tgtattgtac tggtggaata agatgtgaaa aagctagtgc
     4321 ttacatgctg cacaaaggat ttcaaaatgt ttttcattta gaaggtggaa ttataaatta
     4381 tgctaagcaa attaaagaag agggagaacc cagtaagttt attggtaaga attttgtatt
     4441 tgataacagg ctaggagaaa gaattactag tgatgttatt gcaaaatgtc atcaatgtgg
     4501 agctacatgc gatgagcata cgaattgtaa aaataatggt tgtcatttac tatttataca
     4561 atgtaaaaac tgtgcaaata aatatgcagg atgctgttct gaagcatgta aagaaacaat
     4621 taatatgtcg gaagaaaggc aaaaagaatt aagaaaaggg actgaaaacg gaataatgat
     4681 ttttaataaa agtaaagcaa ggttaaggcc caaattgaat gaatctaaac cttagtaaag
     4741 gttaatcgta atttttattt tgaccagcat tagcccattg cacatttaat attccttttc
     4801 ttgcttggtc attggcaaaa tatacaaatg caattacttt taaattagcc gagtttaatc
     4861 cattaatatc taccgtataa tttgcaatgt actcattgtc ttttgtttga ttggctacag
     4921 gaacagtaat tcctttaata cttgtaggag acaaagaata aacaccatta tgcacaaaag
     4981 tatttattgt atctcctgca gaaaagaatg gatttccagg gtaacttaaa ttgttattat
     5041 aataatttgc ttgtggagct attttattat tttctacaac taaaattact agttttaaac
     5101 tgtctttaat ggttacatca aatcctgttt ttgctgtaat atctaaatta ttgccattta
     5161 ctgttgaatt tattgctaac ccaactacag ctcttttttg ataaaatttt ttaaattcag
     5221 tactatcatt taagttattt acatcgccat tttcttgcat aaatgaagta cgattaatta
     5281 atacatttgg taccgatgaa actccataat catttctcat gatagaatca acagttctat
     5341 tagccaaatt gtcgttacta tgaatttgaa cagcaattaa attattattt gctgccataa
     5401 agttatttag tttataatcc attctagggc accaaccaca ccaggttgca gtataaaatt
     5461 ctgccaatac tttagtagaa tattttgctg ctgttggtgc atttgtagta actgttagtg
     5521 tatcgctaat tacgttgtac ttgtttgcat aaactttatg agtgccttgt tgattagctt
     5581 caaaaaaaac tgcagagcct ttaattgcaa aaccatcaac aaaaataacc gacgaagcag
     5641 aaatatcatt tccatcttgg tctttaacgg taacttttat ttcatcaata ccatcggcag
     5701 ttactgaagt tttatctaat gaaatactta ctgaagttgg tattggatta acagtacttt
     5761 catttttatt gcaagaaaaa atcaaaatgg ctaacgatgt gtaaatcaag ttaaaagtaa
     5821 atcttctcat gtaattatta gttgctgcaa acataaatta ttgtgatgct aaaaagcagt
     5881 taattttaat tttttaccat ttatttttaa gatttaattt aaggtagagt atttcctata
     5941 attaacatta tgttaagttg tcatttgata attaatatca ctaattagtt ataaatcaaa
     6001 taaaccagta tttaatgctt taagagtttg attttttaaa tttcttggta ctagtaaaga
     6061 tatattgtgt ccacttcctc cataacttac catggtaaca ggtatttggg aaatggctga
     6121 aaatatttta ggcataattt cattctgagc atttatttca ttgccaacaa tagatattat
     6181 tgtttgctca aaaactaatt caactgaact aaattctgat aattctaaaa taattttttt
     6241 aagatgtgta ttattatcaa tagttacact cacagcaact tcagacgttg taaccatgtc
     6301 tattgaagtt ttatactttt caaatacttc aaaaattctt tttaaaaacc catatgccag
     6361 caacattctg ctacttttaa ccttaattgc aataatgcca tcttttgctg caacagcttt
     6421 aacacctttt gtaggtttat tatcatttat taaggtacca aatgcagatg gctgcatagt
     6481 atttaatagt ttaactggaa tattaaaatg ttgtgcaggc caaatacatg cagggtgtaa
     6541 aattttagct ccaaaatagg ctagttctgc agcttcatca aaactaagtt gttctattgc
     6601 ataagtttta tctactattc ttggatcatt gttatgtaag ccatcaatat ctgtccaaat
     6661 ttcgcaaaca gatgcgttaa ttgcagctgc aattaatgaa gctgtataat cgctgccccc
     6721 tctttttaaa ttatctattt ctccttttgc atttctacaa atatatcctt gtgtaacaaa
     6781 tatggtacag tttgtatgtt gttgtaaaag ttgagtgagt ttaactttta tacttcctat
     6841 ttgtggttct tcattgttat caatcgtcat aaattctaaa gctggtaaaa gggtatgttg
     6901 tatgccaact tcacttaaat aggttgagaa caatttagta cttagcaact ctccttgtgc
     6961 taaaatatct ttgcttaatg cttcgctaaa agatattttt aatatgatat ttaaaaaatc
     7021 aaaatgctca cttataaatt cataagcttt tgcttgatat tcttctttac ttaataaatc
     7081 tataataaaa tttttgtaat gaatttctag ttcatcaatt ttttgtttag ctgtttccct
     7141 atttccttgt tctaataaat tacaaatttc aattaatgag ttggttgtac cactaagtgc
     7201 acttaaaact ataatttttg gttgattgtt ttgactaatt agtgtagcca cttgatgcat
     7261 tctttctggc ttaccaacac tagttcctcc aaacttcatt actttcatct gtaaaatttt
     7321 tattgttcta atgtatatat aggtattgct tacaaacaat tactgcctga tagccccccc
     7381 ccatagtctt agttaattga aaattaattg gttgcaaatt tagctaccaa atttaatttt
     7441 taaaattttt tttaggagta ttagttaggc tgttttgtgt gtaggatgtt tatttaacag
     7501 ctgtagttgc tcctaaccaa gccatcattg caataccgtt ttcaatggct tgttcatcaa
     7561 tattaaattt tggtgtatgc acattatgaa taatgccttt taattcattt cttactccta
     7621 acctaaaaaa acatccagga atttgttgtg agtaatatcc aaaatcttcg gctcccattc
     7681 ttaattcagt ttcttctaca tgttctttgc cccaaaaatc atcagcaagt tgccatgctg
     7741 tggtagtaag ggcttcatca ttatctacag ttgggtagcc aatatcaatt ttaatattta
     7801 ttgtagcacc catactttca gcaatagctg ttgcatgctt ggttattagt tgatgagctt
     7861 tgtaacgcca agtttcatcc atagctctta aagtacccat taatttcact tcgctaggaa
     7921 taatatttgt agctgtacca ccatttattg cacaaaaaga taaaactgat ggaatagtag
     7981 gattattatt tcttgaaatg atttgttgaa gtgaaacgat aattgtagaa gcaattaaaa
     8041 cggtatctgt tgttaaatgt ggtgctgctg catgacctcc attaccatta attgtgatgt
     8101 aaatttcatc tgcacttgcc attactcttc ctttcctaaa actaaattta ccaattggta
     8161 aacctggatg aacatgcatg ccaattattc catctggttt gggattgttt aaaacccctt
     8221 cttttatcat gatacttgct ccaccaggat ttttttcttc tcctggttga aaaattaatt
     8281 taatagtacc ctcccactct gttttagttt cttgtaaaat tttagctgcc cctaataaac
     8341 aagttgtatg aacatcatgc ccacatgcat gcataatgcc attttttttt gacttgtaag
     8401 gcacaacatt ttcttccata attggtaaag catccatatc tgctctaagt gcaattactc
     8461 ttttgctagg gttattccct tctattaaag caactacacc agttgtagca agtgtgctac
     8521 taactatacc aaatgaagtt aatttttgtt gaatgaattg gcaggtttca aactcttcat
     8581 aactgagttc tgggtttgca tgaatatgat gacgaagttc tataaactca ttgctatatt
     8641 gtttagctag taatgtaatt ttttctttaa gctgcatagg tttttttatt aaaactcttc
     8701 atctggtggt ggtatataat ctattacatc ttctacaata aataccccat ttttatatgt
     8761 tttatttaaa atttttctaa atttttctgc atcttctctt tttacaaaat ttcctattct
     8821 aactctaaag taaggagatt gaaataacac gtaagatttt tcttctggat attttgtaag
     8881 taattctgct ttaatattaa atgctttttc tctctctcta gtgcttataa tttgtaacct
     8941 aaatcctttg tattgccctg tgctggtaag catagaactt cgtttattaa taattatttg
     9001 cttttgagtt aatatatcta acctactatc tttatgaaca ataatagtat ctgaggcata
     9061 taaaaactga ccacaacaaa acaataatat aaaaagccaa aatactttca tgttaatttt
     9121 attttttaat aacctgcttt tgctccacca atattttcaa taggatgcca ggttgtttta
     9181 atttcctgag catctaaaat taaatatgga ttttcagctt cgctattgtt ccaattaata
     9241 tcgttgtata atagaactct ttttaagttt aaagcagctt tatcttgaat tagcttttgt
     9301 gagtctttat cattttcaca atataccatt gcattcacat ccatatggtc ggcaaaatat
     9361 tttactaatt cagaaggctt gcctgttaaa atatttacaa ctccagaagg gacatcacta
     9421 ctattaatta cttctgcaaa agttatacca cataaaggct tttgttcgct agctaaaaca
     9481 atacaagtat taccacctgc aattattgga gcaataacag aaactaagcc taataatgaa
     9541 gaactttgtg gagcaataat acttacaact cccgttggtt ctggtgtaga aaagttgaaa
     9601 tgagaactag caactggatt aacagaactg ctaaactgtt gaaatttatc gcaccaacct
     9661 gcaaaataaa ttaacctatc tattgcagta ttaacttcac tctctgcctg attatgtgat
     9721 gagccttgtt gttttaactc attaataaat tgctcttttc ttccttccag catttctgca
     9781 atacgatata gaatttgtcc tctattaaaa gcagctctac tactccatcc tccaaaagca
     9841 tttcttgcag caattacagc ttctctaaaa tcttttctgc tgcttaaaca aatgttggct
     9901 aatggttttc ctgttttatt aagtggtgta taatatcttc cactttctgt tctaggaaac
     9961 tgtccaccta tatagatttt atatgtttta agaacttgca aacggttcat tttgaattat
    10021 tatttaatga tatgataaaa attattttaa ataagcaccc aagccatgta agccaccttc
    10081 tcttccaaaa ccactttctt tatacccacc aaatggagat gttggatcga atttattaaa
    10141 agtatttgcc catacaactc ctgctctcat tttagtggtt agattaaaaa ttttagagcc
    10201 tttatctgtc caaactcctg cacttaagcc ataaggtgtg ttgttggctt tttcaattac
    10261 ttcatcatct gttctaaaag tttgaatagt aagtactggc ccaaaaattt cttcttgcac
    10321 aattctacta ctttgtgcaa cattggtaaa taaagttggt ctaataaaat atcctttttt
    10381 aggaatactg cattggcttt ggtgcattac tgctccttct ttttcgccaa tttttaaata
    10441 ctttaaaata gtatccattt gttgtttaga gt
//
LOCUS       DAOQHP010000028        10449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3199_length_10449_cov_3.607081, whole genome shotgun sequence.
ACCESSION   DAOQHP010000028 DAOQHP010000000
VERSION     DAOQHP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_10449_cov_3.607081"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="PLY81_01835"
     CDS             complement(<1..1570)
                     /locus_tag="PLY81_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29003.1"
                     /translation="MNFIKKANNLFILSFIVLFVFTSCTKKRYPANTPFVYNNKIILN
                     GNISKDEKKRLKVELQNYWDDSLKPRHSSQWLGVVKKFKNPPVFDTVNFSRTKIFMNA
                     YLQSQGYYYSAFGKDSFYIKKGPLLGQKDQLRTTVLININLSKRIKIDSVKYEFIDSI
                     HHSIKDSVLQNLAMQQYKKSFLKTGEPYTKQKISDELDRLVQWFRTKGYYKFTREHVY
                     ALVDTTNENLFKLTLDPIELAKLIVDAEKKRRENPSWKITIKQRVNDDSTRLNKFYVR
                     NSYYYPETKISEIPDSLINSSSFRISKNKKGDLFLKEKEGLFKIKPLREHTYLRKDSL
                     YNEYSFFKTLNTLSSIPAWQQIDARIIPQGKDSLDIHYFLVPQKKYQISYNIEGSRNS
                     GDFTAGNLLGVSLSTSLNNRNVGKLAIQSNTGLRGGVELNLFQQNSQGTKSDLIQTVQ
                     FSLSQTYSFPRLVMPRSFLKLPLFKRTENKRTVLTGAIAYTERFQIFRLRSANFSYAW
                     EWQKLLKNNYTALIS"
     gene            1576..2298
                     /locus_tag="PLY81_01840"
     CDS             1576..2298
                     /locus_tag="PLY81_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF29004.1"
                     /translation="MLSKNDIKYIQSLCHKKQRGIEKLFIAEGPKILEELLNSSFEIE
                     KIYATKDYLSKLDTYNNNIIEISEIEMNKVSQLSTPSTVIALVKQKPDYYSNLTNKLN
                     LVLDGIQDPGNLGTIIRIADWYGVENIFCTNDTVDWYNNKVIQSTMGSFVRVNIWYGN
                     VEEILTNHKLPVYGAVLNGNNLFENTDAKEGFLIIGNEGKGIRTNLLPVINYPVTIPK
                     KGGAESLNAAVATGILLSWFTK"
     gene            2318..3367
                     /locus_tag="PLY81_01845"
     CDS             2318..3367
                     /locus_tag="PLY81_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF29005.1"
                     /translation="MKIKTALCSFGTSGKFFHAPFIYNHPGFDLYAVVERTNKQAKEK
                     YPNIISYNSFYELINDAAVDVVIINTPNITHFEYATMALHANKHVIVEKPFTTTVKEA
                     QALITLAKEKNKLLTVYHNRRYDSDFLTVQDIVIKKVLGEIHEARFCFDRYKPELSNK
                     IHKETPGVGSGILFDLGSHLIDQTIQLFGLPNAVFADIGKQRTNSKIDDYFDLKLYYK
                     KSSVSLHSSLLVKENNIGYILHGTNGSFIKPKTNIQEEFLLQNLLPTLHNFGAESEFD
                     FGVLNATINNKLVREKVPSVTGNYMQFYTSMYNAIINQAPLPVSAEEAMQVIQIIEAA
                     YISNRERRVIELSTS"
     gene            complement(3364..4221)
                     /locus_tag="PLY81_01850"
     CDS             complement(3364..4221)
                     /locus_tag="PLY81_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF29006.1"
                     /translation="MKLKLSQRIAISYYRAKIKTISLLSKKLAAEKALTIFCTPFSGK
                     PKRAIPHLFHQAEKLVCHTNNLKLVGWQWNAKNSNGKKVLIAHGFDSCSYKFDKLIQH
                     LLSAGFTVLAFDAPAHGLSDGKTVNALDYKKALLAINKQVEDLYCIIGHSLGGLAASL
                     AAEEMHELKKLILIAPATETQRAIDNFFKYVPLGKDIKDEIEKIIYNFEQKPVSYYAV
                     SRSVQTITIPVFWVHDSHDWICPFEDVKPIVDLHLNHIQFYISNGLGHNKIYRNNTVM
                     QKITDFLIH"
     gene            4241..5005
                     /locus_tag="PLY81_01855"
     CDS             4241..5005
                     /locus_tag="PLY81_01855"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNF29007.1"
                     /translation="MNVVMIGSGKVAALLSKKIHNAGLKVLQVYSPNLQHATLLADII
                     NAEATNNIENIIQSANIYIIAIADDSIEGVASKLNVSKKALVLHTSGAVNMNVLKSCS
                     DFYGVLYPIQSISKTTDNNAVLPFAIDANNDIAMETIKFITNALHSNVEILNDLQRLH
                     LHIAAVFVNNFVNFLYFNASNICKDATINFNVLQPLIEETALRLRNNLPEVVFTGPAI
                     RKDFSTINKHLKVLETNEQLFELYYFLTKCILVKES"
     gene            complement(5110..7224)
                     /locus_tag="PLY81_01860"
     CDS             complement(5110..7224)
                     /locus_tag="PLY81_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNF29008.1"
                     /translation="MIACKTNEIEKKTYEWPKDANAPIAEVKPFTRIWHNDTVVDNYY
                     WMIDYFKKGPDSTKVVDYLKTENAYTEAMLHGTKKLQADLFTEMKSRIKEKDESVPTF
                     KNGYYYYSKTEDGKQYFKYCRKKGSLDAPEEILLDVDKMAEGKAYFSVNGFSVSSNNK
                     LLAFGVDEVSRRQYTIYIKNLETGELLKDVIPNTEGSACWAEDNTTIFYTAKHPVTLL
                     SEKIKKHVLGTDAAKDVVVYHEKDNSNYIGISKTKNDKYIYIYSGGTLSSEIFYIDAA
                     KPNDVFKSFQPRIKNVLYSIYALEDKFLILTNNNAINFKLMECPLDKTGIEHWKELVP
                     HRPDILLQDVDVFKNFIVLSERKNGLTQINIKNTKENKEHYLDFGEPTYAAYIGSNEE
                     YNTDKLRYNFTSLTTPNSVFDYDMNSKNKTLLKQQEVLGGYNSKDYVSERLFATAKDG
                     TKIPISIVYKKGFHKNGKSPLLLYAYGSYGNSLDASFSSTRLSLLNRGFAYAIAHIRG
                     GQEMGRQWYEDGKMFKKKNTFTDYIDCAEFLVKENFTSKEHLYAQGGSAGGLLMGAVI
                     NMAPNLWNGVIAQVPFVDVVNTMLDESIPLTTNEFDEWGNPKKKEDYFYMKSYSPYEN
                     IEKKDYPNLLVTTGLHDSQVQYFEPAKWVAKLRAMKTDKNVLLLHTNMEFGHGGASGR
                     FDYLKDIALNYAFILALEGINQ"
     gene            complement(7385..8707)
                     /locus_tag="PLY81_01865"
     CDS             complement(7385..8707)
                     /locus_tag="PLY81_01865"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HNF29009.1"
                     /translation="MGIIKDTFKSKADIANSEIKAILNEHGNKKIGEVTLAQIYQGMR
                     GITGLVSETSLLDAQEGIRFRGYSIPELQAKLPKAEGGNEPLPEGLFYLMLLGELPTE
                     NDVQHLTSVWQRRSHVPSHVFQTIDALPLTAHPMTMFVTGIMALQTESHFAEEYSKGI
                     NKKDYWNPMYDDAMDLIARLPRIAAYVYRRKYKNNQHIQPNGLLDWSGNLAHMMGYND
                     PRFKELMRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQ
                     EVIKWIFEMQEQLGTDSPTKAQIEQYVKDTISSGKVVPGYGHAVLRKTDPRFSAQMEF
                     GKKHMPDDKLCQTVWNIYETVPPILQSLGKIKNPWPNVDAHSGALLVHFGLVEYEFYT
                     VLFAVSRALGVMASLCWDRALGFPLERPKSVTTESVKKWLEGKDEIWD"
     gene            complement(8902..9999)
                     /locus_tag="PLY81_01870"
     CDS             complement(8902..9999)
                     /locus_tag="PLY81_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNF29010.1"
                     /translation="MKKLDWYILKKFFATFFFSILLFAIIAIVIDASEKADDFVQSKL
                     SIKNIITDYYFGFVPHIIALLFPLFVFIAVIFFTSKMAGKSEIIAILASGVSYNKWLK
                     PYFIGGVLLTLFLWIANQNIIPKANVIHASFLTKYVDANSSYEALMSSQRKRDLYFRL
                     DSFSYAGIINYDTSSKSGGQFFLNKFKGTKIVENLRSNEIRWDNEKNKWILTSVLVRK
                     INGLQEEMVEFESKEMNFNFKPFDLRKDTYVKSKLTTEELKRYIQLEEMRGSESINEL
                     KVERYKRDAGAFSVLLLTLIGAIIAGRKVRGGSGMHLAWGFIIAALYIITDKFSTIFS
                     SKGNFQPLIAAWLPNIFFCFVAYFLYRKAPK"
     gene            complement(10188..10376)
                     /locus_tag="PLY81_01875"
     CDS             complement(10188..10376)
                     /locus_tag="PLY81_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29011.1"
                     /translation="MATAKKAAPKKAAPKKAAPKKAAPKKAAPKKAAPKKAAPKKAAP
                     KKAAPKKAAPKKAAPKKK"
BASE COUNT         3483 a         1597 c         1501 g         3868 t
ORIGIN      
        1 aagatattaa ggcagtataa ttatttttaa gaagtttttg ccattcccat gcatagctaa
       61 aattggcaga tctaagtctg aaaatttgaa acctctctgt ataggctatt gcacctgtta
      121 aaacggttcg tttattttcc gttcttttaa ataatggaag ttttaaaaag cttcttggca
      181 ttactaatct aggaaagcta tatgtttgag acaaactaaa ttgtactgtt tgaattaaat
      241 ctgatttagt gccttgtgaa ttttgttgaa ataaatttaa ttcaacacca ccccgcaaac
      301 ctgtattgct ttgtattgca agctttccaa cgtttctgtt attaagagaa gtacttaaag
      361 aaacgcctag taaattacct gctgtaaaat ctcctgagtt tctactacct tcaatattat
      421 agcttatttg atattttttt tggggaacta aaaaataatg aatatctaaa gaatcttttc
      481 cttgtggtat tattctggca tctatttgct gccatgcagg aatagaagaa agtgtattaa
      541 gtgttttaaa aaaactatat tcattataca aactgtcttt tcttaaataa gtatgttctc
      601 ttaatggctt aattttaaac aatccctctt tttctttcaa aaacaaatca ccttttttgt
      661 tttttgaaat tctaaaactt gaactattta ttaaagagtc tggtatttcg ctaatttttg
      721 tttctggata gtagtaagaa tttcttacat aaaatttatt taatcgtgtt gaatcatcgt
      781 ttactctttg cttgatagta attttccaag aaggattttc tcttcgtttc ttttctgcat
      841 caacaattag ttttgctaat tcaatggggt ctaaagtaag tttaaataaa ttttcattag
      901 tcgtatcaac taaagcatat acatgctctc tggtaaactt atagtaacct ttggttctaa
      961 accattgtac cagcctgtct aactcatcag atattttttg tttagtataa ggttcaccag
     1021 tttttaaaaa agatttttta tattgttgca ttgccaggtt ttgcaataca gaatctttaa
     1081 tgctatgatg aatggaatct ataaattcat attttaccga gtcaattttt attcttttac
     1141 ttaaattaat atttattagt acagttgttc gtagttggtc tttttgtcca agcaaaggcc
     1201 ctttctttat ataaaaacta tcttttccaa aagcagaata ataataaccc tgagattgaa
     1261 gataggcatt cataaaaatt ttagttctag agaaatttac agtatcgaaa acaggcgggt
     1321 ttttaaattt ttttactaca cctaaccatt gagaactatg ccttggcttt aaactatcat
     1381 cccaataatt ttgtaactca acttttaatc tttttttctc atctttagat atatttccat
     1441 ttaaaataat tttattatta taaacaaatg gggtattggc aggatatctt ttctttgtac
     1501 aggatgtaaa aacaaacaat actataaagg aaagtataaa gaggttgttt gcttttttaa
     1561 taaaattcat aaactatgct aagtaaaaat gatatcaaat atattcaatc tttatgccat
     1621 aaaaaacaaa gaggcataga aaaacttttt attgccgaag gtccaaagat tttggaagaa
     1681 ctattgaata gtagttttga aattgaaaaa atttatgcaa caaaagatta tttatccaaa
     1741 ctagatacat ataataacaa tattattgaa attagtgaga ttgagatgaa caaagttagc
     1801 caactaagta caccctctac tgttatagct ttggtaaagc aaaagcctga ttattatagc
     1861 aatttaacca ataaactaaa tttagtttta gatggcatac aagacccagg aaatttagga
     1921 acaattataa gaattgctga ttggtatgga gttgaaaata ttttttgcac taatgataca
     1981 gtagattggt ataacaataa agtaatacaa agcacaatgg gcagttttgt aagggtaaat
     2041 atttggtatg gtaatgtgga agaaattctg actaaccata aattacctgt ttatggtgca
     2101 gttctaaatg gaaataattt atttgaaaat acagatgcga aggaagggtt tttaattata
     2161 ggaaatgagg gaaaaggaat aagaacaaac ctattgccag tcatcaacta tcctgtaaca
     2221 atacctaaaa aaggtggtgc agaaagttta aatgcagcag ttgctacagg tattttatta
     2281 agttggttta caaagtaatg gcaaacaaat accaattatg aaaattaaaa cagccttgtg
     2341 tagttttggc actagtggaa aattttttca tgcaccattt atttataatc atcctgggtt
     2401 tgatttgtat gctgttgtag aaagaacaaa caagcaagca aaagaaaaat atcctaacat
     2461 tatttcttac aatagttttt atgaattaat aaatgatgct gctgttgatg tagttatcat
     2521 taacacacca aatattactc attttgaata tgctacaatg gctttgcatg ctaataagca
     2581 tgtaatagtt gaaaaaccat ttactacaac agtaaaggag gcccaagcat tgataactct
     2641 agcaaaagaa aaaaataaat tattaaccgt ttatcataac agaagatatg atagcgattt
     2701 tttaactgtt caagatattg ttataaaaaa agtattgggc gaaatacatg aagcaagatt
     2761 ttgttttgat aggtataaac ctgaattaag caataaaatt cataaagaaa caccaggtgt
     2821 aggttctggt attttgtttg atttaggctc tcacttgata gaccaaacaa ttcaattatt
     2881 tggactacca aatgcagttt ttgctgatat aggtaaacaa agaaccaata gcaagattga
     2941 tgactacttt gacctaaaac tttattataa aaaatctagt gttagcttac atagtagttt
     3001 attagttaaa gaaaataaca ttggatacat tttacatggc actaatggtt catttattaa
     3061 acctaaaacc aatattcaag aagagttttt attacaaaac ttgttaccca ctttacataa
     3121 ttttggggca gaaagtgagt ttgattttgg tgtattaaat gctactataa ataataaatt
     3181 agttagagaa aaagtaccat cagttacagg taactatatg cagttttata catctatgta
     3241 taatgcaata attaaccaag ctccattacc agtaagtgca gaagaagcaa tgcaagttat
     3301 tcaaattata gaagcagctt atataagcaa cagagaaagg agagtgatag aattatctac
     3361 ttcttagtga atcagaaagt cagtaatctt ttgcataact gtattatttc tataaatttt
     3421 attatgcccc aatccgttgg aaatataaaa ttgaatatgg tttaaatgta aatctacaat
     3481 aggttttaca tcttcaaaag ggcaaatcca atcatggcta tcatgtaccc aaaatacagg
     3541 aatggtgata gtttgaacac ttctgctaac agcataatag ctaacaggct tttgctcaaa
     3601 attgtaaatt attttttcta tttcatcttt tatatctttt cctaagggta catacttaaa
     3661 aaaattatca attgctcttt gggtttctgt tgctggtgca attaaaataa gtttcttcaa
     3721 ttcatgcatc tcttctgctg ctaaagaagc agctaaacca cctagcgaat gaccaatgat
     3781 acaatataaa tcttctactt gcttgtttat tgccagtaac gctttcttat agtctagtgc
     3841 atttacagtt ttgccatcgc ttaaaccatg tgcaggagca tcaaaagcta gtactgtaaa
     3901 acctgcactc agtaagtgtt gtataagttt atcaaattta tagctacagc tatcaaaccc
     3961 atgtgcaata agtacttttt tcccattact attttttgca ttccattgcc aaccaacaag
     4021 ttttaaatta ttagtatggc aaaccaattt ttctgcttga tgaaataagt gtggtattgc
     4081 tcgttttggt tttccgctaa atggtgtaca aaaaattgtt agtgcttttt cggctgctaa
     4141 ttttttagat aaaagtgaaa tggttttaat tttggctcta taatagctta ttgctattct
     4201 ttgcgataac tttaacttca taaaaattca aatatacgat atgaatgtgg taatgattgg
     4261 ttcaggaaaa gtagcagctt tactttcaaa aaaaatacac aatgctgggc tgaaggtatt
     4321 gcaagtatat agcccaaact tgcaacatgc aacactatta gctgatatta taaatgctga
     4381 agctactaac aacatagaga atattataca aagtgcaaac atttatataa ttgcaattgc
     4441 agatgacagt attgaaggag ttgcaagcaa attgaatgtt tcaaagaaag cattggtatt
     4501 acacacatct ggggctgtaa atatgaatgt attgaaaagt tgtagtgatt tttatggggt
     4561 attatatcct attcaaagca tttccaaaac taccgataat aatgcagttt tgccttttgc
     4621 tatagatgcc aataatgata ttgcaatgga gacaataaaa tttattacaa atgcacttca
     4681 ttctaatgtt gaaatattga acgacttgca acgtttgcac ttacatattg cagctgtatt
     4741 tgtaaacaat tttgtaaact ttttatactt taatgcaagt aatatttgta aagacgctac
     4801 aattaatttt aatgttttac aaccattaat agaagaaact gcattaaggt tgagaaacaa
     4861 tttacctgaa gtagtattta caggccctgc aatcagaaaa gattttagta caattaataa
     4921 gcatttaaaa gttttagaaa caaatgagca actatttgaa ttatattatt ttttaactaa
     4981 gtgcatttta gttaaagaaa gctagaaaat tttagaatga aatttttata aatagaaaat
     5041 aataaagaaa atcctttaga taatttacta aaggattttt aattagttaa ttttcattaa
     5101 gttttttgat tattggttta taccttctag tgctagtata aaggcataat ttaaagcaat
     5161 gtcttttaaa tagtcaaaac gtccacttgc tccgccatgt ccaaattcca tattagtatg
     5221 cagtaaaaga acatttttat ctgttttcat tgctcttaat ttagcaaccc atttagcagg
     5281 ctcaaaatat tgaacttggc tatcgtgtaa gcctgttgta actaataaat taggatagtc
     5341 tttcttttca atattttcat aagggctata acttttcata taaaaataat cctccttttt
     5401 tttagggttg ccccattcat caaactcgtt tgtagttaaa ggtatggatt cgtcgagcat
     5461 tgtatttaca acatctacaa acggaacctg tgcaattact ccattccata aattaggagc
     5521 catattaata actgcaccca ttaataaacc tcctgcacta ccaccttgtg catacaaatg
     5581 ttctttgctt gtaaaatttt ctttaactaa aaattctgca caatcaatat aatcggtaaa
     5641 agtatttttc tttttaaaca ttttaccatc ttcataccat tgccttccca tttcttgtcc
     5701 tcccctaata tgtgcaatgg cataagcaaa acctctgttt aataaactta atctggtact
     5761 gctaaaacta gcgtctaaac tgtttccata actaccgtaa gcatataata ataaaggcga
     5821 ttttccattt ttatgaaatc cttttttata tacaattgaa ataggaattt tagttccatc
     5881 tttagcagtt gcaaataatc tttcagaaac ataatcttta gaattgtatc cacccaaaac
     5941 ttcttgttgc tttaatagag ttttgttctt actattcata tcataatcaa aaacagagtt
     6001 gggcgtagtt aaagaggtga aattgtacct taatttatct gtattgtatt cttcgttact
     6061 gcctatatat gctgcataag taggttctcc aaaatctaaa taatgttctt tattttcttt
     6121 tgtgtttttt atatttatct gtgttaatcc attttttctt tcagataaaa caataaagtt
     6181 tttaaataca tctacatctt gtaacaaaat atcaggtctg tgtggcacca attctttcca
     6241 atgttctatt cctgttttat ctaatgggca ttccattaat ttaaaattaa tagcattgtt
     6301 attagtaaga attaaaaatt tatcctctaa tgcataaatg gagtataaaa cattttttat
     6361 tcttggctga aaggatttaa aaacatcatt aggctttgct gcatctatat aaaatatttc
     6421 agatgataat gtgccaccac tgtaaatata aatatactta tcgttctttg tttttgaaat
     6481 tcctatgtag tttgaattat ctttttcatg atacactaca acatcttttg cagcatctgt
     6541 acctaaaaca tgttttttaa ttttttcaga taatagcgtt actgggtgtt ttgctgtata
     6601 aaaaatagta gtattgtctt ctgcccaaca tgcagatcct tctgtatttg gaataacatc
     6661 tttaagcaat tcacctgttt ctaagttctt aatataaatg gtgtattgtc ttctacttac
     6721 ttcatctact ccaaatgcta aaagtttatt attactgcta accgaaaagc cattcacaga
     6781 aaaatatgct ttaccttctg ccattttatc tacatctaat aatatttctt ctggtgcatc
     6841 taagctacct ttttttctac aatacttaaa gtattgtttt ccatcctctg ttttgctata
     6901 ataataataa ccatttttaa atgtaggtac cgattcatct ttttctttta ttctactttt
     6961 catttcagta aataaatcag cttgtagttt tttagtgcca tgtaacattg cttctgtata
     7021 agcattttct gtttttaaat aatctacaac ttttgtgcta tctggccctt tcttaaagta
     7081 gtcaatcatc caatagtaat tatccactac tgtatcgtta tgccaaattc tggtaaatgg
     7141 ttttacctct gcaataggtg catttgcatc tttgggccat tcataagttt tcttttctat
     7201 ttcgtttgtt ttgcaagcta tcatactagt tgttattagt agaattaata ttttcttgta
     7261 cataaaataa atttttaagc ttattgcaat gtagtaaaaa ttaatttctt tagtttaatt
     7321 atatataagt ggtaaaaaaa agaagctact gtataagtag cttctgaatt aggataaaaa
     7381 aatattaatc ccaaatttcg tctttaccct ctaaccattt tttcacactt tcggtagtta
     7441 cactttttgg tctttctaaa ggaaaaccta gtgctctatc ccaacataag cttgccatta
     7501 cacctaatgc acggcttact gcaaataaaa cggtataaaa ttcgtattct accaaaccaa
     7561 aatgtactaa taaagctcca ctatgtgcat ctacatttgg ccaagggttt tttatttttc
     7621 ctaaagattg taaaatagga ggaacagttt cataaatatt ccaaacggtt tggcaaagtt
     7681 tatcatcggg catgtgtttt ttaccaaatt ccatttgtgc tgaaaaacgt gggtctgttt
     7741 ttctaagtac tgcatggcca taacctggta caactttacc tgaagagatg gtatctttca
     7801 catattgttc tatttgtgct ttggttgggc tatctgttcc taattgttct tgcatttcaa
     7861 aaatccattt aattacttct tgattagcta agccatgtaa agggccagct aaaccattca
     7921 taccagctgc ataacttaaa taaggatcgc ttaatgcact accaactaaa tgggttgtat
     7981 gtgcagatac attacctcct tcatgatctg catgtatagt catgtataaa cgcattaact
     8041 ctttaaatct tggatcgtta taacccatca tgtgtgcaag attaccagac caatctaata
     8101 aaccatttgg ttgtatatgt tgattgttct tatattttct tctgtaaacg taagctgcaa
     8161 tacgtggtaa tcgagcaatt aaatccattg catcatcata cattggattc caataatctt
     8221 ttttattgat accttttgaa tactcttcag caaaatgact ttctgtttgt aaagccataa
     8281 tacctgttac aaacatagtc attggatgag ctgttagtgg taatgcatca attgtttgaa
     8341 aaacatgtga tggtacatga ctacggcgtt gccatacact agttaaatgt tgtacatcat
     8401 tttctgtagg taattctcct aataacatta aataaaacaa tccttctggt aatggttcat
     8461 ttcctccttc tgctttaggg agttttgctt gtagttctgg tatagaataa cctctaaacc
     8521 taattccttc ttgtgcatct aataaacttg tttcagaaac caggcctgtt ataccacgca
     8581 taccttgata gatttgagca agggtaactt ctccaatttt cttgttacca tgttcattta
     8641 atattgcttt tatttcgcta ttggcaatat ctgctttgct tttaaatgtg tccttaataa
     8701 ttcccatttc tttatttttt ttagtttatt atcaagaata aaccttgtta tataatataa
     8761 ctataatcaa aaaagattat aattttcaat ttgtttaata gggatttttt taaataatgc
     8821 tacattatgc aatgtatatt attgaaagtg gttaaagata aagtaagtaa tttgtactac
     8881 aaagtacagt agaagaaaaa tttattttgg tgcttttcta tataaaaagt aagcaacaaa
     8941 acaaaagaaa atattaggta gccaagctgc aataagtggt tgaaaatttc cttttgagga
     9001 aaagatggta gaaaatttat cagtaattat atacaaagca gcaataataa agccccaagc
     9061 taggtgcata ccactaccgc ctcttacttt tcttccagca ataattgccc caattaaagt
     9121 taataaaagc acagaaaaag cacctgcatc acgtttgtat ctttctactt ttaattcatt
     9181 tatagattcg gagcccctca tttcttctaa ttgaatatat cttttaagtt cctcggtggt
     9241 taatttactt tttacataag tatcttttct taaatcaaaa ggtttaaaat taaaattcat
     9301 ttcttttgat tcaaattcta ccatttcttc ctgtaatcca tttatttttc ttaccaatac
     9361 agaagttagt atccatttgt ttttttcatt atcccatctt atttcattgc ttcttaagtt
     9421 ttcaactatt ttagttcctt taaatttgtt taaaaaaaac tgacctcctg atttactgct
     9481 agtatcgtag ttaataatgc cagcataaga aaaagaatct aatctaaagt ataaatctct
     9541 tttacgttgg ctactcatta atgcttcgta agatgagttt gcatctacat atttagttaa
     9601 aaaacttgca tgaataacat ttgcttttgg aatgatattt tgattagcta tccataaaaa
     9661 aagggtaagc aacacaccac caataaaata tggttttaac catttgttgt aggatacacc
     9721 gcttgcaagt attgcaataa tttcactttt tcctgccatt tttgaagtaa aaaaaataac
     9781 tgcaataaat acaaaaagag ggaagagtaa tgcgattata tgtggtacaa aaccaaaata
     9841 ataatcagta attatatttt taatacttag tttggattgt acaaagtcgt ctgctttttc
     9901 actagcatca ataacaattg ctatgatagc aaacaataaa attgaaaaaa agaatgtagc
     9961 aaagaatttt tttaatatat accaatcaag ctttttcata ctttaattac acttggcaaa
    10021 tatacttgca tattactatc aataggttaa agatgaataa tcttacttag gttattttat
    10081 ttaacttgta catggcaatt ttttgtttaa aagccttgct attgcttatt tacattaaaa
    10141 taaaaactcc gatatatatc ggagttttta ttttcttata gaagaaatta tttcttctta
    10201 ggagctgctt ttttaggagc tgctttttta ggagccgctt ttttaggagc tgccttttta
    10261 ggagctgctt ttttaggagc tgccttttta ggagctgctt tttttggagc tgcttttttt
    10321 ggagccgctt tttttggtgc tgcttttttt ggtgcagctt tttttgcagt agccattttg
    10381 ttttaaattt aatggttagt taatgccata aaattataaa cattttttaa actgcaaaat
    10441 tttttttat
//
LOCUS       DAOQHP010000029        10341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3256_length_10341_cov_4.760548, whole genome shotgun sequence.
ACCESSION   DAOQHP010000029 DAOQHP010000000
VERSION     DAOQHP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_10341_cov_4.760548"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLY81_01880"
     CDS             <1..995
                     /locus_tag="PLY81_01880"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF29012.1"
                     /translation="ERKIRAVYLFFFYTLCGSLLMLIGILYIYNQVGTLNLEYLLAWN
                     FTFNEQLFLWFAFFFSFASKIPMFPFHIWLPEAHVEAPTVGSVLLAGILLKLGVYGFL
                     RFSLTIFADASLYFAPLIYLLSVVGVIYASLSALRQTDLKRIIAYSSIAHMNLVTLGI
                     FSFNVIGLEGSIIQSVSHGFVSGGMFLLIGILYDRYHTRFLYYYGGIVHFMPIYAALF
                     LIFTFANIAIPGTSSFTGEFLLLAGIYQTNIITAVVSALGVILCGGYSLWLYNRIVFG
                     NIKVNYTLKFSDVSSREFFIMFPLFIFIFVMGIYPTYFLSYIHLASNAVCLINL"
     gene            1003..1611
                     /locus_tag="PLY81_01885"
     CDS             1003..1611
                     /locus_tag="PLY81_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29013.1"
                     /translation="MNNKINNYNQCQSLLYLSKCDLIHKLSLTNINECPEIERVSIIF
                     SSKQLKEKAPTRKEISLRIIGFLFVYTLLGLNPKIQYVKEKTVDYSSKQPKNAYFNQI
                     VSLDNKYDITKFIHFLFVQNSLKNNIGSTVVDKRKISNSTLELTAIFPISVFQDAIEY
                     ASSEVVDLVAKDLEIKVVFYIKNAEKLGNNNFSILSNFWHIG"
     gene            1606..1690
                     /locus_tag="PLY81_01890"
     tRNA            1606..1690
                     /locus_tag="PLY81_01890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Ser,seq:tga)
     gene            1721..3709
                     /gene="nuoL"
                     /locus_tag="PLY81_01895"
     CDS             1721..3709
                     /gene="nuoL"
                     /locus_tag="PLY81_01895"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF29014.1"
                     /translation="MLFLIILLPILGFFSGSLFGRVLGKGVCFITTFLTFTSCLISIS
                     LLIDVINSGNVYILVLKEWIYSDTIKVDWSFCFDSLTCVMLVIVTFISSLVHLYSTEY
                     MENDPHLPRFMSYLSLFTFFMLILVTANNFLQMFVGWEGVGVSSYLLINFWFTRIQAN
                     KAAIKAMLVNRVGDFFLLLGMFTIYLVFNTLDYDVVFSLVPLALGQKVIIGTYQINVI
                     DLICIFLFLGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVIRCSYIF
                     EYSSVGLNFVTLIGAMTAFFAATTGLFQNDMKRVIAYSTCSQLGYMVFACGLSSYEVG
                     IFHLSNHAFFKALLFLGAGSVIHAMSDEQDMRKMGGLKNLLPFTYAIMLIGSLALIGF
                     PFLAGFYSKDVILEVSASTYTTVGQFAFILGILAAFCTAFYSTRLLYLVFLANPNGNK
                     NVTLHAHEGSWRMTLPLFILSILSVVVGYVSKDMFIGFGTNFWGTSIFINPVNYTMTD
                     IEFLDIQYKLLPLIVTILGVYLAYFIYTYKLTDYYLFKKSDLAKSIFNFFNKKWYFDR
                     LYNEHFVQNILSLSYHYFYQNIDRGFVEKLGPSGISLAIVKFVETIRVYQTGFMLHYL
                     AYFFSAIVLFIVLATKDSLLIFILLLTYLYLLPNVIKK"
     gene            3720..3794
                     /locus_tag="PLY81_01900"
     tRNA            3720..3794
                     /locus_tag="PLY81_01900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3754..3756,aa:His,seq:gtg)
     gene            3809..3892
                     /locus_tag="PLY81_01905"
     tRNA            3809..3892
                     /locus_tag="PLY81_01905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3843..3845,aa:Leu,seq:taa)
     gene            3921..4763
                     /gene="coxB"
                     /locus_tag="PLY81_01910"
     CDS             3921..4763
                     /gene="coxB"
                     /locus_tag="PLY81_01910"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNF29015.1"
                     /translation="MKMLTDLLKAMNVIAYCDAPEAWQLGVQDPATPVMEGMLFFHNY
                     LLFFLIVIGVFVCWLLTYVVVNFNEHTNPVAQKFTHSSILEIVWTILPALVLLLIAVP
                     SFALLYSLDELIDPAVTLKVIGHQWYWSYEYADYTGLDYLDNGVIINFDSYMVPTNDL
                     VKGSFRLLEVDNRVVLPINTHIRVLVTAADVLHCWAVPSFGIKLDACPGRLSQTSLFI
                     KREGVYYGQCSEICGVNHGFMPIVVRGVSFDRYTDWVANKIQEIISEKNSSLAYSKLE
                     QLAS"
     gene            4860..6020
                     /locus_tag="PLY81_01915"
     CDS             4860..6020
                     /locus_tag="PLY81_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HNF29016.1"
                     /translation="MSKFRWNKDSLLSFIDSHIIDYPTPVNLNYLWSFGSAAGICLVI
                     QILTGIFLAMHYCPHIDLAFNSVEHIMTDVRNGWLIRYTHANGASMFFIVVYCHIFRG
                     LYYGSYIYPRGRLWASGVIIFLLMMATAFMGYVLPWGQMSFWGATVITNLFSAIPVVG
                     PSIVEWLWGGFSVGNATLNRFFSLHYLMPFAIVGLVLVHLALLHKDGSNNPLGINTNT
                     DNVPFYPYFYVKDLFAFFILALMFSFFVFFYPNVLGHSDNYIPANPLSTPAHIVPEWY
                     FLPFYAILRSIPDKLGGVVAMISAILVLLLLPVINTSKIRSSKFRPIFGVAYWFLVSD
                     FIILGWIGQKPVESPYIEIGMGATVFYFVFLLVLVPVIGIIESSLIQKDSSK"
     gene            6184..8211
                     /gene="nuoG"
                     /locus_tag="PLY81_01920"
     CDS             6184..8211
                     /gene="nuoG"
                     /locus_tag="PLY81_01920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNF29017.1"
                     /translation="MIHIKIEDVELLVKPQISILEACKFAGITVPRFCYHETLSVSGN
                     CRMCLVEIEGLEKPMASCVAEVDEGMSIWVNSPFVKKARENVSESLLLNHPLDCPICD
                     QAGECDLQDQIKTFGSSYSKFFFNKTSTEDKNCGPLIKTIMTRCIKCTRCVRYSTEVA
                     GVDYFGTMNRGNGTEIGSYVEKMFESEISGNVIDLCPVGALTSKPYAFKARPWELRLS
                     ESIDTTDSLGSNIYVNYKETEIFRILAKNNNDLNGSIISDRARFSYDANNNNRINSIY
                     DFSSKTKGYNELSWKAFFENIDTLAKNNTLQILVDEKSDVELLIALKKIANIYSDKIK
                     LNFISRTNSKGNLYIANKTNIINEINNVSDLIVLLGSNLRLENAILNATIRFRYKHSL
                     LSINSFGLNFSENIPTNFINLSITKLLNVFEGKSLRNSASLIKAKKPLILIGENFADR
                     VDNIDNFVSVVSNILPQAKIISLNNISNSEGLNFLNIDNNKLNSSDYVIAINLDDNIV
                     NRRLLKSSNNYLWVNTHGSALATKFQTIIPSLTSFESEQIFINLEARPQKTQKAFHSF
                     FDARKSVDIILAIFNLNYDIIKTKVENYYSFVFEQVSNLELYSSLKTIKSPFNFVTKQ
                     ISIKTSTPVVISKYFIKQKSKDFYCFDTFTRNSITMQNCSSEVKKNQTNFY"
     gene            8263..8334
                     /locus_tag="PLY81_01925"
     tRNA            8263..8334
                     /locus_tag="PLY81_01925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8296..8298,aa:Cys,seq:gca)
     gene            8355..>10341
                     /locus_tag="PLY81_01930"
     CDS             8355..>10341
                     /locus_tag="PLY81_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNF29018.1"
                     /translation="MYKLIKDLNKEFGFYYKKDFLKKLKRNTKSNYINYYCDFETVLF
                     NDKHYISCFSIVNKNVQIVKSINISSISELESASNRLLKDFIENCFSIAHKKNRFYFH
                     NLSRFDSFFLINLISKEKTSNYLVELYNPNGNLYKLKLICNKTGFILYFYDTFHLLPF
                     SLDVIGQKFCNSTKKIWDHTLSIENYLDNVTLMSSLRDYCLNDALILYKSLENFSQLI
                     YKEFKIDIFNSLTISSFSLKLFRLFYYDDIKNKIQLLTEPVDNFVRQSYHGGYTDVFK
                     PFCNKGYYYDINSLYPYVMSNNVFPGEISSFFEINPIGNTSFDINTFFGFIEVEVFAP
                     ENLMKPFLVYKDKDKGLIAPLGTWTGVYFSEEIKYALKLGYTFKYIKGIEFNKIEPFK
                     DFVNDLYSKRCVCNDTDPLKQIYKLILNSIYGRFGMITDDHKSILCEEEIFDYLLNTR
                     VVVDYSVLNNLYSVSYKEQSKFDLHYSIWQNLTSNKSNTDFDSVFKSLISNYTPIYKK
                     NINLISHVSSAITAYARIYMHQFINNNNVYYTDTDSIVTDFKLDQNLVSDTEIGKFKL
                     VHEFKEAYFINPKVYALKKSETDIILKSKGQAFFDSYHIYEELYKTPGYYTENFNFFQ
                     RNLKTYEVLKVKNSGFFSNYFLKRNKIYKNNCWVDTEP"
BASE COUNT         3395 a         1125 c         1520 g         4301 t
ORIGIN      
        1 gtgaaagaaa aatacgtgct gtttacttat ttttctttta taccttgtgc ggttctttac
       61 taatgcttat tggtatttta tatatttata atcaagtagg tactttaaat ttagaatatt
      121 tattagcttg gaatttcact tttaatgagc aattattttt atggtttgca ttcttttttt
      181 cttttgcatc taaaatacca atgtttccgt ttcatatatg gttaccagaa gctcatgttg
      241 aggctcctac tgttgggtct gttttactag ctggtatttt attaaaattg ggtgtttatg
      301 gatttttaag gtttagttta actatttttg cagatgcttc attatatttt gcgcctttaa
      361 tttatttatt atctgttgtt ggtgtaattt atgcatcttt aagtgcttta agacaaactg
      421 atttaaaacg aatcattgct tattcttcta ttgctcatat gaacttagtt actttaggta
      481 tatttagttt taatgttatt ggtttagagg gttctattat tcaaagtgta agtcatggtt
      541 ttgtttctgg aggtatgttt ttattgatag gtatattata tgataggtat catactagat
      601 ttttatatta ttatggtggt attgtccatt ttatgcctat ttatgctgca ttatttttaa
      661 tatttacttt tgcgaatatt gcaattccag gtactagcag ctttacagga gaattcttat
      721 tattagctgg tatttatcaa actaatatta ttactgctgt tgtaagtgca ttaggtgtta
      781 ttctatgtgg aggttattct ttatggttat acaatagaat tgtatttgga aatattaaag
      841 taaactatac tttaaaattt agcgatgtat cttctcgtga attttttata atgtttcctt
      901 tatttatatt catatttgtt atgggtattt atccaactta ttttttatct tatattcatc
      961 tagcaagcaa tgctgtttgc ttaattaatt tgtaataaaa ttatgaataa taaaattaat
     1021 aattataatc aatgtcagtc acttttatat ttgtcaaaat gtgatttaat tcacaaatta
     1081 agtctaacaa atattaatga gtgccctgaa atagaaaggg tttctattat tttttcttca
     1141 aagcaattaa aggaaaaagc ccctacacgt aaagaaatta gcttaagaat tattggtttt
     1201 ctttttgttt atactttatt aggcttaaat cctaaaattc agtatgtaaa agaaaaaaca
     1261 gttgattatt catctaagca accaaaaaat gcttatttca atcaaattgt aagtttagat
     1321 aataaatatg atattactaa atttatacat tttttatttg tccaaaacag tttaaaaaat
     1381 aatataggtt ctactgttgt tgataaaaga aaaattagta actctacttt agaattaact
     1441 gctatttttc ctatttctgt atttcaagat gctattgaat atgcttcttc tgaagttgtt
     1501 gatttagtag caaaagattt agaaattaaa gtagtttttt atattaaaaa tgctgaaaaa
     1561 ttaggaaata ataatttttc tatactttct aatttttggc acattgggta aatggctgag
     1621 cggtttaagg cgttagtctt gaaatctaaa gtatcgtaag ataccgtggg ttcaaatccc
     1681 actttgccct tcgattattt aatgaaaatg aacaaaaaat atgttgtttt taattatatt
     1741 attaccaata cttggttttt ttagtggtag tttattcggg cgtgttttag gtaaaggtgt
     1801 ttgttttatt acaacatttt taacttttac ttcttgttta atttctatta gtttgttaat
     1861 agatgttatc aattctggta acgtttatat attagtttta aaagagtgga tttattcgga
     1921 tacaataaaa gtagactggt ctttttgttt tgatagctta acttgtgtaa tgcttgttat
     1981 tgttactttt atttcaagtt tagttcatct ttattcaaca gaatatatgg aaaatgatcc
     2041 acatttacca agatttatgt cttatttatc actttttacc ttttttatgc taattttagt
     2101 aactgcaaat aattttttac aaatgtttgt tggttgggaa ggtgttggtg tttcttctta
     2161 tcttttaata aacttttggt ttactagaat acaagcaaat aaagctgcta ttaaagctat
     2221 gcttgttaat cgtgtaggag atttttttct attattaggt atgtttacca tctacttagt
     2281 ttttaatact ttagattatg atgttgtttt tagtttagta cctcttgcat taggtcaaaa
     2341 agttattatt ggtacttatc aaataaatgt tatagatctt atttgtattt ttttattttt
     2401 aggtgcgatg ggtaaatcag ctcaattagg attgcatact tggttacctg atgcaatgga
     2461 aggtcctacc cctgtttcag cattaattca tgctgctact atggttactg ctggtgtttt
     2521 tttagttatt cgttgttctt atatttttga atattcttct gtaggtttaa attttgttac
     2581 tttaataggt gcaatgactg ctttttttgc agctactact ggattatttc aaaatgatat
     2641 gaaaagggta attgcttatt cgacttgtag tcagttaggt tatatggtat ttgcttgtgg
     2701 tttatcaagc tatgaggttg ggatttttca tttatcaaat cacgcttttt ttaaagcatt
     2761 attattttta ggagctggat cagtaattca tgcaatgtca gatgaacagg atatgcgtaa
     2821 aatgggtgga ttaaaaaatt tattaccatt tacttatgct attatgttaa taggatctct
     2881 agctttaata ggatttcctt ttttagcagg tttttattct aaagatgtta ttttagaggt
     2941 aagtgcttct acctatacaa ctgtaggtca gtttgctttt attttaggta ttttagctgc
     3001 attttgtact gctttttatt ctactcgttt attatactta gtgtttttag caaaccctaa
     3061 tggtaataaa aatgtaactt tacatgcaca tgaaggttct tggagaatga ctttaccttt
     3121 atttatttta tcaattttaa gtgttgttgt aggttatgtg tctaaggata tgtttatagg
     3181 atttggtaca aacttttggg gaacttcaat ttttattaac cctgttaatt atacaatgac
     3241 tgatattgag tttttagata ttcaatataa actattacct cttattgtta caattttagg
     3301 tgtgtattta gcttatttta tctatactta taaactaaca gattactatt tatttaaaaa
     3361 atctgattta gcaaaatcaa tttttaactt ttttaataaa aaatggtact ttgatagatt
     3421 atataacgag cattttgttc aaaatattct atctttaagt tatcattatt tttatcaaaa
     3481 tattgataga ggttttgttg aaaaattagg tccttctgga atttctctgg ctattgttaa
     3541 gtttgtagaa actattagag tatatcaaac tggatttatg cttcattatt tagcttattt
     3601 ttttagtgct attgtattat ttattgtttt agcaacaaaa gatagcttat taatatttat
     3661 tttactttta acttatttat atttgttacc aaatgtaatt aaaaaataaa taaatatttg
     3721 gtggatatat ctcaattggt agagagtcaa gttgtggcct tgaatgttat gggttcaaat
     3781 cccattattc acctttaatt atattatagc ccagttggcg gaattggtag acgcgacaga
     3841 cttaaaattt gttccttact caagggtgtt ggttcgagtc caataccggg catatttaat
     3901 ttttaacgga ataaatatat atgaaaatgt taacagattt gttaaaagct atgaatgtta
     3961 tagcgtattg tgatgcacca gaagcatggc aattaggggt tcaagatcct gcaactcctg
     4021 taatggaagg gatgcttttt ttccataatt atttattatt cttcttaatc gttattggtg
     4081 tttttgtttg ttggttatta acttatgtag tagtaaattt taatgaacat actaatccag
     4141 tagcgcaaaa atttactcat tcaagtatat tagaaattgt atggactatt ttacctgctc
     4201 ttgttttatt attaattgca gttccttcat ttgctttatt atactcttta gatgagttaa
     4261 ttgatccagc tgttacttta aaagtaattg gacatcaatg gtattggagt tatgaatatg
     4321 ctgattatac tggtttagat tatttagata atggtgtaat tattaatttt gattcttata
     4381 tggttcctac taatgattta gttaaaggat cttttagatt attagaagta gataatagag
     4441 ttgttctacc tattaatact catatacgag ttttagttac tgctgctgat gtattacatt
     4501 gctgggcagt tccttcattt ggtattaagc ttgatgcttg cccaggacgt ttaagccaaa
     4561 cttctttatt tattaaaaga gagggtgttt attatggaca atgtagtgaa atttgtggtg
     4621 taaatcatgg attcatgcct attgttgtaa gaggtgtttc ttttgataga tatactgatt
     4681 gggttgctaa taaaattcaa gagattataa gtgaaaaaaa tagttcatta gcttattcta
     4741 aacttgaaca attagcaagt taatataaaa tacaacagta atattaactt aacttatctt
     4801 acctatattc ttaattgaat attaaaaatt agaaaatttt aaaataaaaa aaataaatta
     4861 tgtcaaagtt tagatggaat aaagattctt tattatcttt tatcgattcg catattatcg
     4921 attatccaac cccagttaac ttaaattatc tgtggagttt tggatcagct gctggtattt
     4981 gtttagtaat tcaaatactt accggtattt ttttagctat gcattactgt cctcatattg
     5041 atttagcatt taatagtgtt gaacatatta tgaccgatgt tagaaatggt tggttaatta
     5101 gatacaccca tgcaaatgga gcttctatgt tttttatagt ggtatattgt catattttta
     5161 gaggtttata ttatggatca tatatttatc caagaggtag attatgggct agtggtgtaa
     5221 ttattttctt attaatgatg gcaactgctt ttatgggtta cgttttacct tggggtcaaa
     5281 tgagtttctg gggtgcaacc gtaattacta atttattctc tgcaattcca gttgttgggc
     5341 cttctattgt tgaatggtta tggggaggat tctctgtagg aaatgctact ttaaatagat
     5401 tttttagttt acattaccta atgccttttg ctattgttgg tcttgtttta gtgcatttag
     5461 cattattaca taaagatggt tctaataatc cattagggat taatactaat acagataatg
     5521 ttccttttta tccttatttc tatgtaaaag atttatttgc attttttatt ttagctttaa
     5581 tgttttcgtt ttttgttttt ttttatccaa atgttttagg acatagtgat aattatattc
     5641 cagcgaaccc attatctact cctgctcata tagttccaga atggtatttt ttaccttttt
     5701 atgctatttt aagatctatt cctgataaat taggaggtgt tgtagctatg ataagtgcaa
     5761 ttttagttct tttattatta cctgttatta atacttctaa aattagaagc agtaaatttc
     5821 gtcctatttt tggggtggct tattggtttt tagtttctga ttttattatt ttaggttgga
     5881 ttggtcaaaa acctgtagaa agtccttata ttgaaattgg tatgggtgca actgtatttt
     5941 actttgtatt cttattagtt ttagttccag ttattggaat tattgagtca tcattgattc
     6001 aaaaagattc ttcaaaataa aatatttata ttataagaac gttttaaaac cttaaatata
     6061 gtaaatatat ttagatagat actttataag atctatttac agttaaaacg acggctaata
     6121 atcttttaat tatatattat aattaaatat gttattttat ttttttaatt agaagccaaa
     6181 caagtgatac atatcaaaat agaagatgta gaacttttgg ttaaaccaca aatttctatt
     6241 ttagaggcgt gtaaatttgc tggaattaca gttcctcgtt tttgttatca tgaaacttta
     6301 tctgtttctg gtaattgtcg tatgtgtctt gttgaaatag aaggattaga aaaaccaatg
     6361 gcatcttgtg ttgctgaagt tgatgaaggt atgtctattt gggttaatag tccttttgtt
     6421 aaaaaagcaa gagaaaatgt ttctgaatct ttacttttaa atcatccttt agattgccct
     6481 atttgtgatc aagctggaga gtgtgattta caagatcaaa ttaaaacttt tggaagcagt
     6541 tatagtaaat ttttttttaa taaaacttct actgaagaca aaaattgtgg tccattaatc
     6601 aaaacaatta tgactagatg tattaaatgt acaagatgtg tacgttattc tactgaagtt
     6661 gcaggtgttg attattttgg tactatgaac agaggtaatg ggactgaaat tggttcatac
     6721 gtagaaaaaa tgtttgagtc tgaaatctca ggtaatgtta ttgatctttg tccagtagga
     6781 gctttaactt ctaagcctta tgcttttaaa gcacgtcctt gggaacttcg tttgtcagaa
     6841 agtatagata ctactgattc tttagggtct aatatttatg ttaattataa agaaacagaa
     6901 atttttcgta tattagcaaa aaataataac gatttaaatg gtagtattat ttcagataga
     6961 gctagattta gttatgatgc aaataataat aatagaatta attctattta tgatttttca
     7021 tcaaaaacta aaggttataa tgaattatct tggaaagctt tttttgaaaa tatcgatact
     7081 ttagctaaaa ataatacttt acaaatacta gttgatgaaa aaagtgatgt tgaattatta
     7141 attgctttaa aaaaaatagc aaatatttat tctgataaaa taaagttaaa ttttatttca
     7201 agaacaaatt caaaaggtaa tttatatatt gccaataaaa caaatattat taatgaaatt
     7261 aataatgtaa gtgatcttat tgtattatta ggatctaatt tacgtttaga aaatgctatt
     7321 ttaaatgcaa caatacgttt tagatataaa catagtttac tttcaattaa ctcatttggt
     7381 ttaaatttta gtgaaaatat tcctactaat tttattaatt taagtattac aaaattatta
     7441 aatgtttttg aagggaaatc tttaagaaat tctgcctcat taataaaagc taaaaaacct
     7501 ttaattctta taggtgaaaa tttcgctgat cgagtagata atatagataa ttttgtttct
     7561 gttgtttcta atattttacc tcaagcaaag attatttcat taaataatat ttctaactca
     7621 gagggtctta attttttaaa tattgataat aataaattaa actcatcaga ttatgttatt
     7681 gctattaatt tagatgataa tattgttaac agacgtttat taaaatcatc aaataattat
     7741 ttatgggtaa atacacatgg ttctgcttta gcaactaaat ttcaaacaat tattccttca
     7801 ttaacctcat ttgaaagcga acaaattttt ataaatttag aagcaagacc tcaaaaaact
     7861 caaaaagcat ttcattcatt ttttgatgca agaaaatctg ttgatattat tttagctatt
     7921 tttaatttaa attatgatat tattaaaact aaagtagaaa attattattc atttgtattt
     7981 gaacaagtat ctaatttaga attgtatagt tctttaaaaa caattaaatc tccttttaat
     8041 tttgtaacca agcaaatatc aataaaaaca agtactcctg tagtaattag taaatatttt
     8101 attaaacaaa agtctaaaga tttttattgt tttgatactt ttacaagaaa ttctattaca
     8161 atgcaaaatt gttcttctga agtaaaaaaa aatcaaacaa atttttatta aattagattt
     8221 atatcataac tttattgtta tgttctaatg aaattttaat acgatgggat aacataattg
     8281 gtaatgtgtt tgattgcaaa tcaaataaag agggttcaaa tccctctctc atctagaact
     8341 acaattggtg tgttatgtat aaattaatta aggatttaaa taaagaattt gggttctatt
     8401 ataaaaaaga ttttttaaaa aaattaaaaa gaaatacaaa atcaaattat attaattatt
     8461 attgtgattt tgaaacagtt ttatttaatg ataaacatta tattagttgt ttttctattg
     8521 ttaataaaaa tgttcaaatt gtaaagtcta ttaatatttc ttcaatttct gaattagaga
     8581 gtgcttctaa tagattacta aaagatttta tagaaaattg ttttagtata gcgcataaaa
     8641 agaatagatt ttattttcat aatttatcca gatttgatag ctttttttta attaatttaa
     8701 tatcaaaaga aaaaacatca aattatttag ttgaattata taatcccaat ggtaatttat
     8761 ataaacttaa acttatttgt aataaaacag gttttatttt atatttttat gatacatttc
     8821 atttattacc attttcattg gatgttattg ggcaaaaatt ttgtaactca acaaaaaaaa
     8881 tttgggatca tactctttca atagaaaatt atttagataa tgttacccta atgtccagtt
     8941 tacgagatta ttgtttaaat gatgctttaa ttttatataa aagccttgaa aatttttcac
     9001 aattaattta taaagaattt aaaattgata tttttaatag tttaaccatt tcttcatttt
     9061 ctctaaaact atttaggtta ttttattatg atgatataaa aaataaaatt caattgttaa
     9121 ctgaaccggt tgataatttt gttagacaaa gttatcatgg aggttatact gatgttttta
     9181 aacctttttg taataagggg tattattatg atataaactc tttatatcca tatgtaatgt
     9241 caaataatgt ttttcctggt gaaatttcat ctttttttga aattaatcct attggaaata
     9301 catcttttga tattaatacc ttttttggct tcattgaagt tgaggtgttt gcgcctgaaa
     9361 atttaatgaa accctttttg gtttataaag ataaagataa gggtttaatc gcacctttgg
     9421 gtacttggac tggtgtttat ttttctgaag aaataaaata tgcccttaaa ttgggttata
     9481 cttttaaata tattaaaggt attgaattta ataaaataga accttttaaa gattttgtta
     9541 atgatttata ttccaaacga tgtgtgtgta atgatacaga tcctttaaaa caaatttata
     9601 aattaatttt aaattccatt tatggtaggt ttggtatgat aacagatgat cataaatcta
     9661 ttttatgtga ggaagaaatt tttgattatt tacttaatac aagagtagtt gttgattatt
     9721 cagtattaaa taatttatat tcagtgtcat ataaagaaca aagtaagttt gatttacact
     9781 attctatttg gcaaaatctt acatctaata aatctaatac agattttgat agtgttttta
     9841 aatctttgat ttcaaattac acacctatat acaaaaaaaa tataaatttg atttcgcatg
     9901 tatcatccgc gattacagct tatgctagga tttatatgca tcaatttatt aataataata
     9961 atgtttacta tacagataca gatagtatag ttactgattt taaactggat caaaacttag
    10021 tttctgatac tgaaataggt aaatttaaat tggttcatga atttaaagaa gcttatttta
    10081 taaaccccaa agtttatgct ttaaaaaaat ctgaaacaga tattatatta aaatctaaag
    10141 gccaggcttt ttttgattct tatcatatat atgaagagct ttataaaacc ccaggttatt
    10201 atacagaaaa ttttaatttc tttcaaagaa atcttaaaac ttatgaagtt ttaaaagtaa
    10261 aaaattctgg ttttttttct aattattttt taaaacgtaa taaaatatat aaaaataatt
    10321 gttgggttga tacagaacct c
//
LOCUS       DAOQHP010000030        10190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3323_length_10190_cov_3.163493, whole genome shotgun sequence.
ACCESSION   DAOQHP010000030 DAOQHP010000000
VERSION     DAOQHP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_10190_cov_3.163493"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLY81_01935"
     CDS             <1..276
                     /locus_tag="PLY81_01935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF29019.1"
                     /translation="GSNCAISWNVQIMDTDIHTLVVNGQPKESTKKIKIGNNVWIGSN
                     VIILKGAEIGNNVVIAAGSIVSGNIPDNVLIAGVPAKIVKEQISWLL"
     gene            561..1607
                     /locus_tag="PLY81_01940"
     CDS             561..1607
                     /locus_tag="PLY81_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNF29020.1"
                     /translation="MNNPFFEIAGRKIGYDYEPLVIAEIGINHEGSLQTAKEMVDAAK
                     AAGIEIVKHQTHIVEDEMSSAAKNTIPGNATVSIYEIMKRCSLNEEDELEMKNYVESK
                     GMIFISTPFSRAAADRLHKWNIPAYKIGSGECNNYPLLEHIASFGKPIILSTGMNTIE
                     SIAKAVAIFKKHHVPYALLHTTNLYPTPNHLVRLGAMTEIQKNFPDAVVGLSDHTLTN
                     HACLGAVALGASILERHFTDTMDRPGPDIICSMDINAAKELIEGSKIIALQRGGEKGP
                     AAEEQVTIDFAFATICTIKPIKAGEVFTKENIWVKRPGKGGILAEEYNNILGKKALVN
                     IDNDMQLQYSFIEK"
     gene            1611..3041
                     /locus_tag="PLY81_01945"
     CDS             1611..3041
                     /locus_tag="PLY81_01945"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNF29021.1"
                     /translation="MLFNSWVFVLFWIVFFFLYWFVFNKSIKLQNAFIAIASFVFYGW
                     WDWRFLLLLWLSLIVDFCVGYFLEIIENKSKRKLLIVLSIAFNIGLLMFFKYFNFFSN
                     SFESLAHSFGWQVDFVTLNLILPVGISFYTFQSLSYTIEVYNKKLKGTKDIFAYAAFI
                     SFFPQLVAGPIERAANMLPQYYKKRVFNYTQSVKGMQLILWGLFKKVVVADTCAIFAN
                     NAFNNYTQHDGFNLIMGAVFFSFQIYGDFSGYTDIARGLGKILGFELKINFMYPYFSR
                     SIAEFWRRWHISLSSWFRDYVYIPLGGSRSSKAITIRNTTVVFLVSGFWHGANWTFIV
                     WGALHAIYYVPLVIINKHKSFADTVAFNRTLPSFKELSFMIGTFILALIAWVFFRADS
                     IEHAFAFLKGMTNFKGFHNFGLIRCLPFISIMLLVDWVGRNNECALSDFAAKFPKWFR
                     YVIYLFVAVCIYANFSGEQEFIYFRF"
     gene            3045..4001
                     /locus_tag="PLY81_01950"
     CDS             3045..4001
                     /locus_tag="PLY81_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29022.1"
                     /translation="MQIFIKKIIIFIGLFLGISTLVALGTGQLALSKLSRKGRGVVID
                     KNNRLDSITKNKIVLIGGSNVCYGINSQMIEDSFNMPVVDMAINAGMGMSFYYQQVKH
                     AIKAGDIIIGIPEYYPYDGKITNGDAGIYSLAIIDYRNIKFFNAMQWLQLPIYLGDII
                     NENVKAFFTYEKNKITFGRFQYNKWGDYIGHKNEGSYADTFKHNKQYDLSVYENKNEI
                     YPDKNFINLIKNFEQFCNSKNIIYLHAYPVYSRYFYNDNVAKQIAEKIQPLKFINNPE
                     TYLYGFDSLYDTPNHILYKFRDERTSKLINDIRVALHQIKHE"
     gene            3994..5109
                     /gene="neuC"
                     /locus_tag="PLY81_01955"
     CDS             3994..5109
                     /gene="neuC"
                     /locus_tag="PLY81_01955"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNF29023.1"
                     /translation="MNKKIVFLTGTRADFGKLKSLIEISNKNFEVHLFATGMHMDKKY
                     GYTVKEIEKCGYKNIFKFINHDASSMMDITLSRTIEGFANYIHLVQPDLIIIHGDRIE
                     ALAGATVGALNNILVAHIEGGEVSGTVDELIRHAVSKLSHIHLVANDEAKKRLIQMGE
                     NAESIYIIGSPDMDAMLSEDLPTIDEVKNNYEINFEKYAVSMFHPVTTEYDTMDEYAN
                     EYIKALEESNLNYIIIYPNNDKGSEFILNKIKRVEQNNKFRIFPSVRFEAFLVLLKNA
                     QFIIGNSSAGIREAPYYGIPTVNVGTRQNGRTSNPDIINTTYNSTSIINAIQEAKVKK
                     VTPRHLFGDGKSDEKFFQIISSQELWNTPKQKIFSDF"
     gene            5123..5971
                     /locus_tag="PLY81_01960"
     CDS             5123..5971
                     /locus_tag="PLY81_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF29024.1"
                     /translation="MSDSRIVTVVDAKNKTFFFNFKELIRYKDLFYYMVLRDVTVMYK
                     QTVLGFLWAILNPFFQVVVFSVVFGTIAGIKTGIAGLPYPVFSCLAIIPWNYFSSTFS
                     ASSNSLVNTAGVFTKVYFPRLIIPMVPIFSKLVDFFISLIILVLVMGYYQYTPGSNWY
                     LFLLPLLIIILSSAGLGIWLSSLVLQYRDFRFAVNFLLPLLLYAAPVAFPALKVKEKL
                     GNTVYELYALYPMVGGIEGFRSSFVQNADFPWTLVLISGASALFIFISGAFYFKKMEK
                     HFADVA"
     gene            5961..7247
                     /locus_tag="PLY81_01965"
     CDS             5961..7247
                     /locus_tag="PLY81_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29025.1"
                     /translation="MLHNDNNIAVKVTDLGKAYRIGVVEEKEENLSAVILKAILSPVK
                     NFRKIKSLKNISSEATGKDVFWANRNISFEVKKGEVLGIIGKNGAGKSTLLKMLSRIT
                     EPTEGRIELVGRVASLLEVGTGFNPELTGRENIFLNGTILGLTKKEIEERYESIVEFS
                     GIEKFIETPVKRYSSGMKVRLAFAVAAHLDPEILIIDEVLAVGDAEFQKKCLGKMQDV
                     AGKEGRTVLFVSHDMAAVKNLCNRAILLQHGSIVFEGSANDVVNYYLQNAASITSQSN
                     LDFSQAKGNKNFVVTDIEMLDENNNNIQIAESGRDFQLKISYETKEVAINPVVIILVK
                     NNLEQVLFTCLSRNSYNGTMQLNSQGSIICNIPKLPLLSGLYTLDIVLKYDQEVTFSL
                     ENIFSFEVEKGDFFGTGKINDDMMNGMIINHNWVIQ"
     gene            7250..8641
                     /locus_tag="PLY81_01970"
     CDS             7250..8641
                     /locus_tag="PLY81_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNF29026.1"
                     /translation="MQKKRLLITISFSFSIRYIVRTGLLEKIKAFAEPVIVLTWNEED
                     LIKELRNKGYEVYILPELIRGTAYKDIRKKIDYWFVHFRLKSPTKKNQDKFLKQYNSF
                     KQNFIAQSRKLYNIAKMYLPGVQTKMFEKEKKLLISDTNYHQILKWVKPLNINAVFTV
                     TPFHTQEDILLRVCNDLGMKMITSILSFDNITKRGWIPVNYNVYMVWNKYNFNELVRI
                     YKGINKDKIFITGAPQFDFYYNPNFLLTKEEWMKTVGIKDATKKIILYAGGPKSLFPN
                     EPQYLQHINDAINNNEIEGSPIVLFRCHPVDILQRWKDSVGSSNNIVFDSSWTGEKNL
                     VSTNITEFDIKKLCSTLYYTDVHINLCSTMTVDGSAFNKPQIGPAYDDIAPAKAHLLK
                     GMYWQEHFIPVLKVNGIALANSKQELIELTNKALKHSDMDEQKCKQVVEEIITYANGN
                     STQRVAAILENEI"
     gene            8686..9942
                     /locus_tag="PLY81_01975"
     CDS             8686..9942
                     /locus_tag="PLY81_01975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF29027.1"
                     /translation="MKITHVLFGYHPSVGGTQIFYKGISENCVAWYKDEVEVLTTNSY
                     YGPHTNSFREISIPEETINGVLVKRYPFRRNHKKIIGFLNKLVAKITNKQSNYFIEML
                     TGPLSKQLFKALNSTNADVISASSLGYKYMQYPLYRHTLKTPKPFVFQGAIHFKSDEH
                     DGGITPKMLQAIKASEYYLCNTAYEKNRLTALGVNQENIVITGCPVNITQYENAKGHE
                     VRKQLNLSSNDVLIGYIGRLEATKSIPVLINAFEAAFQKNNQLHLVIAGFETAYLKQL
                     KQIVAKLDANIQCKISFLISISENEKANLFQAIDVLVLPSTNESFGIVFLEAWACKKP
                     VIGTAIGAVSSVISNGTDGLLMQPYNTIDLTEKILQLSNDKNLREFMGANGYNKVIQN
                     YTWEIVTKKFRDTYVAAIEKFKKNNF"
     gene            9963..>10190
                     /locus_tag="PLY81_01980"
     CDS             9963..>10190
                     /locus_tag="PLY81_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29028.1"
                     /translation="MFREEAIWINNALAKIKPLQDNNLVANLGSSTAYFREVIQPHIQ
                     QYLMQPLANNGWNIINIDLKQEDGVDVVADVT"
BASE COUNT         3739 a         1287 c         1735 g         3429 t
ORIGIN      
        1 ggcagcaatt gtgccatttc atggaatgtt caaattatgg atactgatat tcatactttg
       61 gttgtaaatg gacaaccaaa ggagtcaact aaaaaaataa aaataggaaa caatgtttgg
      121 attggctcaa atgtaatcat attaaaaggt gcagaaattg gtaataatgt tgtaatcgct
      181 gcaggctcta ttgtaagtgg caacatacca gacaatgttt taattgcagg tgtaccagct
      241 aaaatagtta aagagcaaat tagttggctg ctataaaaat ttgaattatg aaaatggtta
      301 gttcgttttt gtttagtgaa gtttttgaaa agaaaatttt attgagtcaa attatcctaa
      361 atatgtaaga gttaattggg caaaaataaa aataaacaaa taatacagaa gttgattttc
      421 taaaaatcta aaaatagatt acctacaact attcattatt aaatatcaaa taaatgatag
      481 ggtttaaaaa aataataaaa aagtacaagt ctatttttga caattacaac attaaattaa
      541 aaaattagaa aagtatataa atgaataatc ccttttttga aatagcaggc agaaaaattg
      601 gttatgatta tgaaccattg gtgattgcag aaattggcat taaccacgaa ggctctttac
      661 aaacagctaa agaaatggtt gatgcagcta aagcagcagg aattgaaatt gtaaaacatc
      721 aaacacatat tgtagaagat gaaatgagca gtgctgcaaa aaacaccatt cctggcaatg
      781 ctactgtaag catttatgaa ataatgaaac gttgcagcct caacgaagaa gatgaattag
      841 aaatgaaaaa ttatgtagaa agcaaaggca tgatttttat ttctacacct tttagtcgtg
      901 ctgctgcaga tagattacac aaatggaata ttcctgctta taaaattggt agtggcgagt
      961 gtaataatta tcctttgtta gaacatattg catcatttgg taaacccatt attttaagta
     1021 caggaatgaa tactattgaa agtattgcaa aagctgtagc catttttaaa aaacaccatg
     1081 tgccttatgc tttattgcat acaaccaatt tatatcctac acccaaccat ttagtacgtt
     1141 taggagctat gactgaaatt cagaaaaatt ttcctgatgc agtagtaggt ttaagcgatc
     1201 atacattaac caatcatgct tgtttgggag cagttgcatt gggtgcaagt attttagaaa
     1261 ggcattttac agatacaatg gatagacctg gtccagatat tatttgcagc atggacatta
     1321 atgcagctaa agaactaata gaaggcagta aaataattgc tttacaacgt ggaggagaaa
     1381 aaggtcctgc tgctgaagaa caagttacta ttgattttgc ttttgcaaca atttgcacca
     1441 ttaaacctat aaaagcagga gaggttttta ccaaagaaaa tatttgggta aaacgccctg
     1501 gtaaaggggg cattttagct gaagaatata ataatatttt aggcaaaaaa gcgttagtaa
     1561 atattgataa tgatatgcag ttacaatatt cattcataga aaaataatgt atgcttttta
     1621 attcttgggt ttttgtatta ttctggatag tttttttctt tctgtattgg tttgtattta
     1681 ataaatctat taaactacaa aatgccttta ttgcaatagc aagttttgta ttttatggtt
     1741 ggtgggattg gcgtttcttg ttattacttt ggctaagttt aatagtagat ttttgcgtag
     1801 gttattttct tgaaataata gaaaataaaa gcaaacgaaa attattaatt gtattaagta
     1861 ttgcctttaa tataggctta cttatgttct ttaaatactt taatttcttt tcaaattcat
     1921 ttgaaagttt agctcattca tttggttggc aagtagattt tgtaacgcta aatttaattt
     1981 tacctgtagg catatctttt tatacttttc aatctttaag ctataccatt gaagtttata
     2041 ataaaaaatt aaaaggcaca aaagatattt ttgcttatgc tgcattcata agtttctttc
     2101 ctcaattagt agcaggtcca atagaacgag cagcaaatat gttgcctcaa tattataaga
     2161 agcgtgtgtt taattacaca caaagtgtaa aaggaatgca gttgatttta tggggtttat
     2221 ttaaaaaagt tgtagttgca gatacctgtg ccatttttgc taataatgcg tttaataatt
     2281 atacccaaca tgatggattt aatttaataa tgggagctgt atttttttca tttcaaattt
     2341 atggcgattt tagtggttat acagatatag caagaggctt aggtaaaatt ttaggttttg
     2401 agttgaaaat aaatttcatg tacccctatt tttcaagaag catagcagag ttttggagaa
     2461 gatggcatat ttcactttca tcatggttta gagattatgt ttatattcct ttaggtggct
     2521 caagatcttc taaagctatt acaatacgaa atacaactgt ggttttttta gttagtggat
     2581 tttggcatgg tgctaattgg acatttattg tttggggggc tttacatgca atttattatg
     2641 ttcctttggt aattattaat aaacataaat catttgccga tacagttgct tttaatagaa
     2701 ctttaccatc tttcaaagag ctatcattta tgataggtac ttttatttta gctttaattg
     2761 cgtgggtttt ctttagagca gattcaatag aacatgcttt tgcattttta aaaggcatga
     2821 caaactttaa aggctttcat aattttggat taataagatg tttgccattt atttcaataa
     2881 tgcttttagt agattgggta ggtcgtaaca atgaatgtgc tttatctgat tttgctgcaa
     2941 aatttccaaa atggtttagg tatgtcattt atctatttgt agctgtttgt atttatgcta
     3001 atttttcggg agaacaagaa tttatttatt tcagatttta gactatgcaa atttttataa
     3061 aaaaaataat aatcttcatt gggctatttt taggcatttc tactttagtt gcattaggta
     3121 cagggcaact tgcgttaagc aaacttagta gaaaaggtag aggggttgta atagataaaa
     3181 ataacagatt agattctata actaaaaata aaatagtact tattggtggt tctaatgttt
     3241 gttatggcat caactctcaa atgattgaag actcattcaa tatgcctgtt gtagatatgg
     3301 ctattaatgc tggtatggga atgagtttct actatcaaca agtaaaacat gctataaaag
     3361 ctggtgatat tattattggc attccagagt attatcctta cgatggaaaa attaccaatg
     3421 gcgatgcagg aatttatagc cttgcaatta tagactaccg aaacataaag ttttttaatg
     3481 ctatgcaatg gcttcaatta cccatttatt taggtgatat tataaacgag aatgtgaaag
     3541 catttttcac ttacgaaaaa aataaaataa cttttggacg ttttcaatac aataaatggg
     3601 gcgattatat tggtcataaa aatgaaggtt cttatgcaga tacttttaag cataataaac
     3661 aatacgattt aagtgtttat gaaaataaaa atgaaattta tccagataaa aattttatta
     3721 acttaattaa aaattttgaa caattctgca attccaaaaa tataatttac ctgcatgctt
     3781 atcctgtgta ttcaaggtat ttttataatg ataatgttgc aaaacaaata gcagaaaaaa
     3841 tacaaccact aaagtttata aataatccag aaacttattt gtatggtttt gatagccttt
     3901 atgatacccc aaaccatatt ttatataaat ttcgagatga aagaacatca aaactaatta
     3961 atgatattag agtagctcta catcaaataa aacatgaata aaaaaatagt ttttcttaca
     4021 ggcacaagag cagattttgg caaactaaaa tcgttgatag aaatttcaaa caaaaatttt
     4081 gaagtgcatt tgtttgcaac aggtatgcac atggataaaa aatatggcta cacggtaaaa
     4141 gaaattgaaa aatgtgggta caaaaatatt ttcaaattta tcaaccacga tgcatcttca
     4201 atgatggata ttactttaag cagaaccatt gaaggttttg ccaattatat tcatcttgtt
     4261 caacccgatt taatcattat tcatggcgat agaattgaag cattagctgg tgcaactgtt
     4321 ggtgctttaa ataatatttt agtagcacac attgagggag gagaagtaag tggtacagta
     4381 gatgaattaa ttcgtcatgc tgtttctaaa ctaagccata tacatttggt tgcaaacgat
     4441 gaagcaaaaa aaagattaat tcaaatgggc gaaaacgctg aaagcattta catcattggt
     4501 tcgccagata tggacgcaat gctttctgaa gatttaccaa ctatagatga ggtaaaaaat
     4561 aattatgaaa ttaattttga aaaatatgct gtatctatgt ttcatcctgt aacaacagag
     4621 tatgatacta tggatgaata tgctaatgaa tacattaaag cattggaaga aagtaatttg
     4681 aattacataa ttatttaccc caacaatgat aaaggcagtg agtttatttt aaacaaaata
     4741 aaaagagtag agcaaaataa taaattcaga atttttcctt ctgtaaggtt cgaagccttt
     4801 ttagtgctgc taaaaaacgc ccaatttatt attggtaatt caagtgcagg aataagagaa
     4861 gctccttact atggcattcc aactgtaaat gttggtacaa gacaaaatgg aagaacctct
     4921 aatcctgata ttattaatac aacttacaac agcacatcaa tcattaatgc tatacaggaa
     4981 gcaaaagtaa aaaaagtaac acctcgtcat ttatttggtg atggaaaaag tgatgaaaaa
     5041 tttttccaga ttatttcaag tcaagaactt tggaatacac ctaaacaaaa aatattttca
     5101 gatttttaga taaatattca gcatgtcaga ttcaagaatt gtaacagttg ttgatgcaaa
     5161 aaataaaact ttctttttca actttaaaga gttgataaga tacaaagatt tgttttatta
     5221 tatggtgtta agagatgtta cagtaatgta caaacaaaca gtattgggtt ttctttgggc
     5281 aatcttaaat ccattttttc aggtagtagt ttttagtgta gtatttggaa ctattgcagg
     5341 tataaaaaca ggtattgctg ggcttcctta tcctgttttt tcttgtttgg caattattcc
     5401 atggaattat ttttcatcta cattttctgc ttcatctaat agtttagtta atacagcagg
     5461 tgtttttacc aaagtgtatt ttcccagatt aattattcct atggtgccta ttttttctaa
     5521 gctggtagat ttttttattt cactcattat tcttgttttg gtaatgggtt attatcaata
     5581 tacacctggc agtaattggt atttattttt acttccttta ttaataatta tactttcttc
     5641 tgcagggctt ggtatttggc tatcctcatt ggtattacaa tatagagatt ttaggtttgc
     5701 tgtaaatttt ttattgcctt tacttttata tgctgcacct gttgcatttc ctgcattaaa
     5761 agtaaaagaa aaattaggca acacagtgta tgagttatat gctctttatc caatggtagg
     5821 aggcatagaa ggctttagaa gcagctttgt acaaaatgct gatttccctt ggacacttgt
     5881 attaattagt ggagcatctg cattatttat ttttataagc ggtgcatttt attttaaaaa
     5941 aatggaaaaa cattttgccg atgttgcata atgataataa catagcagtt aaagtaactg
     6001 atttaggaaa agcttacaga ataggcgttg tagaagaaaa agaagaaaac ttatctgctg
     6061 taattttaaa agcaattctt tcgcctgtaa aaaattttag aaaaattaaa tcactaaaaa
     6121 atatttcttc cgaagcaaca ggtaaagatg ttttttgggc aaatagaaat atatcttttg
     6181 aagtaaaaaa aggagaggtt ttaggaatca ttggcaaaaa cggggcaggc aaaagcactt
     6241 tgctaaaaat gctttcaaga attactgaac ccacagaagg aagaattgaa ttagtaggaa
     6301 gagttgcaag tttattagaa gtaggtactg gttttaatcc tgaattaaca ggcagagaaa
     6361 atattttttt gaatggaact attttaggct taaccaaaaa agaaattgaa gaaagatacg
     6421 aaagcattgt agagttttca gggatagaaa aatttattga aacacctgta aagcgttaca
     6481 gtagtggtat gaaagttcgt ttagcttttg ctgttgctgc acatttagat cctgaaattt
     6541 taattattga tgaagtgtta gcagttggtg atgcagagtt tcaaaaaaaa tgcttaggaa
     6601 aaatgcaaga tgtggcaggt aaagaaggaa gaactgtatt gtttgttagt catgatatgg
     6661 ctgctgtgaa aaatttatgc aacagagcta ttttattaca gcatggaagt attgtttttg
     6721 aaggaagtgc taatgatgta gtaaattatt atttacaaaa tgctgccagt attacatctc
     6781 aaagcaattt agatttttcg caagccaaag gaaataaaaa ttttgttgtt actgatattg
     6841 aaatgttaga tgaaaataat aacaacattc aaatagcaga aagtggtaga gattttcaac
     6901 taaaaataag ttacgaaacc aaagaagtgg caataaatcc tgttgtaatt attctggtaa
     6961 aaaataattt agagcaggta ttgtttacat gcttatccag aaattcgtac aatggtacaa
     7021 tgcaattaaa tagtcaagga agcattattt gcaatattcc taaactaccc ttattaagtg
     7081 gtttatatac gttagatatt gttttaaagt acgatcaaga agttactttt tctcttgaaa
     7141 atattttttc ttttgaagta gaaaaaggag acttttttgg aacaggaaaa ataaatgatg
     7201 atatgatgaa tggaatgatt attaatcata actgggttat tcaataagta tgcaaaaaaa
     7261 aaggttactc attacaattt catttagttt ttcaataaga tatattgtaa gaacaggatt
     7321 gttagaaaaa ataaaggcgt ttgcagaacc tgtaattgtt ttaacctgga atgaggaaga
     7381 tttaattaag gagttaagaa ataaaggata cgaagtttat attttacctg aattaataag
     7441 aggcacagct tacaaggaca ttagaaaaaa aatagattat tggtttgttc attttagatt
     7501 aaaaagtcct accaaaaaaa atcaagataa atttttaaag caatacaata gttttaaaca
     7561 aaattttatt gcacaaagta gaaaactata caatattgct aaaatgtatt tgccgggtgt
     7621 gcaaactaaa atgtttgaaa aagaaaaaaa acttttaata tccgatacaa attaccatca
     7681 aattttaaaa tgggtaaaac ctttaaatat taatgctgtt tttactgtaa ccccatttca
     7741 tacacaagaa gatattttat taagagtttg caatgattta ggaatgaaaa tgattacctc
     7801 tattctttct tttgataata taaccaaacg tggttggata cctgtaaact ataatgttta
     7861 tatggtttgg aacaaatata attttaatga attggtgaga atttataaag gaataaataa
     7921 agataaaatt ttcattaccg gtgctccaca atttgatttt tattataatc ctaatttttt
     7981 actcactaaa gaagagtgga tgaaaactgt aggaataaaa gatgctacta aaaaaataat
     8041 tttatatgct ggtgggccta agtcattatt tcctaacgag ccacagtatt tacaacatat
     8101 aaatgatgca ataaataata atgagataga agggagccca attgttttat ttagatgcca
     8161 tccagtagat attcttcaaa gatggaaaga tagtgttggc tcttcaaata atattgtttt
     8221 tgattcatct tggacaggag aaaaaaattt agtaagtaca aatattacag agtttgatat
     8281 taaaaagctt tgctcaacac tgtattatac agatgtacat attaatttat gtagtacaat
     8341 gactgttgat ggaagtgcat ttaacaagcc acaaattggc ccagcttacg atgatattgc
     8401 acctgcaaaa gcacatttat taaaaggcat gtattggcaa gagcatttta taccagtatt
     8461 aaaagttaat ggtattgctt tggcaaactc aaaacaagaa ctgattgagc ttaccaataa
     8521 agcattaaag cattcagata tggatgaaca aaaatgtaaa caagtggtag aagaaataat
     8581 tacctatgca aatggaaatt ctacacaaag agtagctgct attttagaaa atgaaattta
     8641 aattgtgtat ttctcaaata aattaaaact tctttaataa cattaatgaa aattacacat
     8701 gttctttttg gctatcatcc atcagttggt ggtacacaaa tattttataa aggtatttca
     8761 gaaaattgtg tagcctggta taaggatgaa gtagaagttt taactactaa ttcttattat
     8821 ggcccacaca ccaacagttt cagagaaatt tctataccag aggaaacaat aaacggagta
     8881 ctagtaaagc gttatccttt tagaagaaat cataaaaaaa ttataggttt tttaaataag
     8941 ctagttgcaa aaattaccaa taagcaatca aactatttta ttgaaatgct tacaggccct
     9001 ctatccaaac aattatttaa agcattaaat tcaacaaatg ccgatgttat ttcagcttct
     9061 tcattaggtt ataagtatat gcaatatcca ctgtacaggc atacacttaa aacccctaag
     9121 ccatttgttt ttcagggagc aattcatttt aaaagtgatg aacatgatgg tggcattaca
     9181 ccaaaaatgc tgcaagcaat taaagcatct gaatattatt tgtgcaatac agcgtatgaa
     9241 aaaaacaggc taacagcgtt aggtgtaaac caagagaata tagtaataac aggttgccca
     9301 gttaatatta cccaatatga aaatgcaaaa gggcatgagg ttagaaagca attgaactta
     9361 tcatctaatg atgtattaat tggttacatt ggaagattag aagcaacgaa aagtattcct
     9421 gttttaataa atgcctttga agcagcattc caaaaaaata atcagttgca tttagtgatt
     9481 gcaggttttg agactgctta tcttaaacag ttaaagcaaa ttgttgcaaa attagacgct
     9541 aatattcaat gcaaaatttc gtttttaata agcatttctg aaaatgaaaa agcaaatttg
     9601 tttcaagcta ttgatgtatt ggtattgcca tctacaaatg aatcttttgg aattgttttt
     9661 ttagaagcat gggcttgtaa aaaacctgta attggcactg caataggtgc tgtaagctct
     9721 gtaatatcaa atggtacaga tggtttgtta atgcaaccat ataacacaat agatttgact
     9781 gaaaaaattt tacagctatc caacgataaa aatttaagag aatttatggg agcaaatggc
     9841 tacaataaag taatacaaaa ctatacatgg gaaattgtaa ccaaaaaatt cagagatacc
     9901 tatgttgctg caatagaaaa atttaaaaaa aataattttt aattcatcaa aaattagaat
     9961 gaatgtttag agaagaagcc atttggataa acaatgcttt agccaaaatt aaaccactac
    10021 aagataataa tttggttgct aatcttggtt catcaactgc atattttaga gaagttatac
    10081 agcctcatat tcagcaatat ttaatgcagc ctttagcaaa taatggttgg aatattatta
    10141 atattgattt aaagcaagaa gatggcgtag atgttgttgc agatgtaaca
//
LOCUS       DAOQHP010000031        10139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3345_length_10139_cov_3.235819, whole genome shotgun sequence.
ACCESSION   DAOQHP010000031 DAOQHP010000000
VERSION     DAOQHP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_10139_cov_3.235819"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1521
                     /locus_tag="PLY81_01985"
     CDS             118..1521
                     /locus_tag="PLY81_01985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF29029.1"
                     /translation="MKLSFHGAAQTVTGSKHLITLKNGKKILLDCGLFQGLGKETGSF
                     NASFGFNAEEVDVMILSHAHIDHSGLIPKLYKEGFRGKIFCTGATFDLTKILLEDSAT
                     IQRDDTRFLNKRRAKSGLPPIEPLYTIEDAEQSFSLLEKQAYQHWFKVTDGVQAMFTD
                     AGHIIGSSSVHLKINEEGKNIRISFSGDVGRYRDLILKSPEVFPQADYIIIESTYGDK
                     LHEDAFNSIDTLLNWVNKTCVEKKGKLIIPAFSVGRTQELLYMLNQLSLENRLPKIPV
                     YVDSPLSIEATEVVKSYPRYFNNRVKKVLDIDEDPFDFPGLNYIKSADESKALNANKQ
                     AMIIISASGMADAGRIKHHIANNIDNNKNTILLVGYCEPHSLGGRLLNGSKEVRIYGE
                     MHHVIAEVGIMRTMSAHGDYDDLSQYLSCQNPALVKSIFVVHGEIDVMHEFKRRLNKK
                     GFKEVEIPKMHESFQLD"
     gene            1565..2341
                     /locus_tag="PLY81_01990"
     CDS             1565..2341
                     /locus_tag="PLY81_01990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF29030.1"
                     /translation="MLKSTLLKAVEDGAKILKEYFDKQFVISNKEGINNLVTEVDHKS
                     EQAIINCIKNSFPNHFILSEEVGEIVQDSEYKWIIDPIDGTVNYANGIPICCVSIGIE
                     KKDEMIMGAVYNPFMNEFYFAEKNNGAFLNNHKIKVSHKTDILSSCLVTGFPYTYLDA
                     TNGPLQIFEKFIRKGIPVRRLGSAAIDLCWVAAGRFDGFYEHKLSAWDSAAGFIIVEE
                     AGGKVTNLNGDFYSPYQPGIIATNGKIHDEMVRIVNGGSL"
     gene            2377..3369
                     /locus_tag="PLY81_01995"
     CDS             2377..3369
                     /locus_tag="PLY81_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNF29031.1"
                     /translation="MNSFGKIFKVQIFGESHGNCVGVVIDGVTAGLPLNEEDFLSDLV
                     RRQGGIQKGTTPRKEIDVPIFKSGIFKDKATGTPLTILFENNNIQSEVYDKQRSIPRP
                     GHVDFTANKKFGGYEDYRGGGHFSARLTTGLVAAGVVAKKMMPEININAVVVEIAGER
                     DLEKGIQKAINEKDTVGGIIECKVLGLPIGLGEPYFDSLESLLAHILFSIPAIKGVEF
                     GSGFNAAKMFGSEHNDTIENKEGKTSSNFAGGIVGGLSNGNELVFRIAVKPASSTPKE
                     QESLNWETNTMEKFSIQGRHDLCVALRAPVIVEAVTAIVLADCMLLEQKIQRVL"
     gene            complement(3364..4047)
                     /gene="radC"
                     /locus_tag="PLY81_02000"
     CDS             complement(3364..4047)
                     /gene="radC"
                     /locus_tag="PLY81_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNF29032.1"
                     /translation="MAGIKDWSEDDRPREKMLLKGANSLSNSELMAILINNGTKDKTA
                     VELAKDLLQSVDNDLRKLSKLTIQDILKLKIKGLGPAKAISIVAALELGIRRDAANKE
                     TQSYSSSNQIALYLKAKFQYLNKEVFAVVYLNNSNKIIKYEIVSEGGITGTVVDNRII
                     LKNALACSATNIVICHNHPSGSLTPSNADKKITDKIKQAANLLDIQVLDHIIVSDEGY
                     YSFADEGML"
     gene            complement(4115..4873)
                     /gene="tpiA"
                     /locus_tag="PLY81_02005"
     CDS             complement(4115..4873)
                     /gene="tpiA"
                     /locus_tag="PLY81_02005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNF29033.1"
                     /translation="MRKQIAAANWKMNCTLQQAEELLTKLSTATVNTTENKISIIGVP
                     APYLLLAKDKLKNKANFYIAAQNCYTKKSGAFTGEISAEMLHSMQITHVIIGHSERRE
                     YFNESNTTLAEKVNIALEYALTPIFCCGEALEIREANNQNSFVENQLKESLFHLSVEQ
                     ILQVVIAYEPIWAIGTGKTASNEQAQEMHQHIRNVLANKYGKTVAEQISILYGGSVKG
                     SNAADLFAQNDVDGGLVGGASLNAEEFTTIINAL"
     gene            complement(4893..6050)
                     /locus_tag="PLY81_02010"
     CDS             complement(4893..6050)
                     /locus_tag="PLY81_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNF29034.1"
                     /translation="MSIILFDTPIRKNFYPITLNKSLGDVRLGIFTLKEWWQQALNKP
                     ILLKTDDYLQPLYEEINDSEEHLFIDASVVPDNNIIHKILALQSSEAIADEFGLIAFK
                     TSNFNNTLSEIFKKIKIIDNRNFVNRITQPFHLFQINEQILKEQFDWIIKQKQSYKIS
                     STNVVINPENIFIDEEANVEHCILNAQNGIIYVGKNATIMEGSIIRGSCAILENAVIK
                     AGTKIYGATTIGKFAVASGEIKNSIISDYSNKAHDGYLGDSFIGSWCNIGAGTNNSNV
                     KNNFNEIFLYKEATNEKVNAGKKCGVIMGDFTRVAINSAINTGSVYGISCNVFGNGLL
                     PKTISNFSWGLNDKYKYSKVIEDINNWMYFKGKQLSDAEIIVLKYIFDNNL"
     gene            6342..9731
                     /locus_tag="PLY81_02015"
     CDS             6342..9731
                     /locus_tag="PLY81_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNF29035.1"
                     /translation="MNFKKVNNITGWIIALIACTVYIVTAEAGGSLWDCGEFVSSCFK
                     LQVPHPPGAPMFVILGRFFIVLLGNDPLTAAKAVNIMSALASGFTILFLFWTITHFAR
                     RIVHSDVSKPLTQTQILSIMGAGVVGALAYTFSDSFWYSAVEGEVYALSSFFTALVFW
                     AILRWEHEADQKGADRWIVFIFFMMGLSIGVHLLNLLTIPAIVMLYYIRKRIDFNYKI
                     IRKYFILFTIIGGSLAFIGTLIVANSEADRVPLDATLAGLALVGTALVIVFLRLVESF
                     KKEKKEYYGGMYIFFILGCIITGVVQVGVIQYTVKMAGAFDRYFVNGFGMPFFSGFAF
                     FFILLAALIWYGLKIAAKKGWYYLKLSLWCVTFMLIGYSTYVTTMIRSNADPAIDMYN
                     VDNPMSLVGYLGREQYGDFPILYGQKFTARPYDSKETGTKYQKGKDNYVEIGKDFKYI
                     YAAEDKMVFPRVWDASNDQSHADYYASFLNIGQMQDGTYERSPNQIDNMHFFLGYQIN
                     FMYFRYFMWNFSGKQNDIQGSSLANLRDGNWITGINFIDNLRLGNQDKMPDSLTHNKA
                     NNKLYALPFILGLIGLLYHFKQKGADALVNMLLFFFTGLAIVVYLNQAGNQPRERDYA
                     YVGSFYAFAVWIGLGVLQVKSWITRVVNQKVAATLATVICLLAVPVLMAQQEWDDHDR
                     SNKTLARDLAKDYLESCPKDAILFCFGDNDTYPLWYAQEVEGIRKDIRVINFSLLGID
                     WYINQLRYKVNESAPIDVIWSADQIEGRKRDYVPFKPNPKFPESAYYDLYDVMKNYIG
                     SDNPAFIDNSRGEPLNTFPVKKFAIPVNKDEIIKNGIVNATDSILAELRFDLNKDRLL
                     KNDLAVLNIIAANKWKRPICFTSNNPYQIGLGFERFLRMDGLTYRLVPVDNSNGTFNT
                     NAPLVNTDWAYDKMMNNFAAGNANVKGVYFDEENRRHLNSIREAYTELAFDLISKNRM
                     NDAKKVLQHCDSLMLQENVPYGMVSRANQHNRNSLRFLTACYEVGDKDLAAKVLKSLK
                     TDIEQQQVYYASLGDGKTSVEAYKKLMDDYITRYNSARTRQDQVTADNILQSNLSQRQ
                     INSLNDIRMSYMLLVELNNVEITNNKTQQVPAQTSVDTTKK"
     gene            complement(9796..>10139)
                     /locus_tag="PLY81_02020"
     CDS             complement(9796..>10139)
                     /locus_tag="PLY81_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF29036.1"
                     /translation="RIKRLEQFGVIKQYATLLDYAKVKKSLMVFCYVSLKEHSKNAGS
                     KFIKHIHSLPEVIECFNISGEFDFMLKVVAESMDDYYHFHVNKLSQSENIANVQSVFV
                     MGVLKQTHIVI"
BASE COUNT         3492 a         1381 c         1715 g         3551 t
ORIGIN      
        1 ggagttttat taaattctat agtatttaaa tattttgtta ggaaaaaaaa gcgttattct
       61 gatttgtagc taagaaattt taaactctag tattcgttaa ttcataaatt ttaaaaaatg
      121 aaattatctt ttcatggtgc tgcacaaact gttactggtt ctaaacattt aattacttta
      181 aaaaatggta aaaaaatttt attagattgt ggcttgtttc aaggcttagg gaaagagaca
      241 ggttcattta atgcaagttt tgggtttaat gcggaggaag tagatgtgat gattctttct
      301 catgcccata ttgaccatag tggccttatt ccaaaattgt ataaagaagg ttttagaggg
      361 aagatatttt gtacaggagc tactttcgat ttaactaaaa tattattaga agattcagct
      421 acaatacaaa gagatgatac aaggttttta aataaaagaa gagccaagag tgggttgcca
      481 cctattgagc ctctttacac aattgaagat gcagaacaat ccttttcatt gctagaaaaa
      541 caagcatatc aacattggtt taaagtaaca gatggggtac aagccatgtt taccgatgct
      601 ggtcatatta ttggtagttc ttctgttcat ttaaaaatta acgaggaagg aaaaaatatt
      661 agaataagtt ttagtggaga tgttggtcgt tacagagatt taattttaaa aagtcctgaa
      721 gtctttcctc aggcagatta tattattatt gaaagtactt atggcgacaa attacatgaa
      781 gatgctttta atagtataga tactttgttg aattgggtta acaaaacttg tgttgagaaa
      841 aaaggcaaac tgattattcc ggcttttagc gttggcagaa cacaagaatt actttatatg
      901 ctaaaccaat taagtttaga aaataggttg cctaaaattc ctgtttatgt ggatagccca
      961 ttaagtatag aagctactga agtagtaaaa agttatccaa gatattttaa taatagagtg
     1021 aagaaagtgt tagatattga tgaagaccct tttgattttc ctggattaaa ctatataaaa
     1081 agtgccgatg aaagtaaagc actaaatgcc aataagcaag caatgataat tattagtgca
     1141 agtggaatgg ctgatgccgg tagaataaaa catcatattg ctaacaatat agacaataat
     1201 aaaaatacca ttttattagt tggctactgc gagcctcata gtttaggagg aagactatta
     1261 aatggatcaa aagaggttag aatttatggc gaaatgcatc atgtaattgc agaagttggc
     1321 attatgagaa ccatgagtgc acatggcgat tatgacgatt taagccaata cttatcttgt
     1381 caaaatcctg ctttagttaa aagcattttt gtggtacatg gagagataga tgttatgcat
     1441 gaatttaaaa gaaggcttaa taaaaaagga tttaaagaag tagagattcc taaaatgcac
     1501 gaaagttttc aattagatta gttagaaact ttcagtaaca tttaattgta atttatattt
     1561 gcctatgcta aaaagtacac tattaaaagc tgttgaagat ggtgcaaaaa tattaaaaga
     1621 atattttgat aagcagtttg taatttctaa taaagaaggt attaataacc tggtaacaga
     1681 agtagatcac aaaagtgagc aagctattat taattgtatt aaaaatagtt ttcctaatca
     1741 ttttatttta agtgaagagg ttggtgaaat agtacaagat agtgaatata aatggataat
     1801 tgaccccatt gatggtactg taaattatgc taatggaata cccatttgtt gtgttagtat
     1861 tggtattgaa aaaaaggatg aaatgattat gggtgctgtg tataacccat ttatgaacga
     1921 attttatttt gctgaaaaaa ataatggagc ttttttaaat aatcataaaa ttaaggtatc
     1981 tcataaaaca gatattttaa gtagttgttt agttacaggc tttccttata cttatttaga
     2041 tgcaacaaac ggccctttgc aaatatttga aaagtttatt agaaaaggta ttcctgttag
     2101 aagattaggc agtgcagcaa ttgatttatg ttgggttgct gccggtagat ttgatggttt
     2161 ttatgaacat aagcttagtg catgggatag tgctgctggg tttattattg tagaagaagc
     2221 aggcggtaaa gtaaccaatt taaatggtga tttttatagc ccttatcaac caggtataat
     2281 tgctacaaat ggcaaaatac acgatgaaat ggtgagaatt gtaaacggag gttctttata
     2341 atttaatcaa gttcaaaaac gcatcccaaa cacatcatga atagctttgg aaaaattttt
     2401 aaagtgcaaa tatttggcga aagtcatggt aattgcgttg gtgttgtaat tgatggcgta
     2461 acggctggat tgccattaaa tgaagaagat tttttatcag atttagttag aaggcaagga
     2521 ggaatacaaa aaggtacaac ccctagaaaa gaaatagatg tacctatttt taaaagcgga
     2581 atttttaaag ataaagctac tggtacacca cttacaattt tatttgaaaa taataatatt
     2641 caaagtgaag tttacgataa acaaagatct ataccaagac caggacatgt agattttact
     2701 gctaataaaa aatttggtgg ttatgaagat tatagaggag gaggacattt tagtgccaga
     2761 cttacaacag gcttagttgc tgctggagtt gtggctaaaa aaatgatgcc tgaaattaac
     2821 attaatgctg ttgttgtaga aattgcagga gaaagggatt tagaaaaagg aattcaaaaa
     2881 gcaatcaacg aaaaagatac agttggtggt attattgaat gtaaagtttt aggcttacct
     2941 attggtttag gagaacctta ttttgactca ttagaatctt tattagcaca tattttattc
     3001 tctattcctg caataaaagg agtagagttt ggaagtggat ttaatgctgc taaaatgttt
     3061 ggaagtgagc ataacgatac tatagaaaat aaagaaggta aaacatcttc caattttgca
     3121 ggaggcattg ttggaggcct aagtaatggt aatgaacttg tatttaggat agctgtaaaa
     3181 cccgcatctt ctacacctaa agaacaagaa agtttaaatt gggaaacaaa tacaatggaa
     3241 aaattttcta tacaaggtcg tcatgattta tgtgtagcat taagggcacc agttattgtt
     3301 gaagcagtaa cagcaatcgt attagcagat tgtatgttac ttgaacaaaa aatacaaagg
     3361 gttttataac ataccttcgt cggcaaaact ataataacct tcatcgctta caatgatatg
     3421 gtctaaaact tgaatatcta ataggtttgc agcttgtttt attttatcgg ttattttttt
     3481 atctgcatta cttggtgtta aacttccact tggatggtta tgacaaataa caatattagt
     3541 tgcactacaa gccaatgcat tttttaaaat tattctatta tctaccactg ttccagtaat
     3601 tcctccttcg cttacaattt catatttaat tattttgttg ctattgttta aatatactac
     3661 agcaaaaact tctttattta aatattgaaa ttttgctttt aagtacaatg caatttggtt
     3721 agaggaagaa taagattgtg tttctttgtt tgcagcatct cttctaatac ccaattctaa
     3781 agctgctaca atactaatag cttttgcagg ccctaatcct tttattttta gttttaaaat
     3841 atcctgaata gttagtttac ttaattttct taaatcatta tctacacttt gtaataaatc
     3901 tttagcaagt tctacagcag ttttatcttt agtgccatta ttaataagta tagccattaa
     3961 ttcagaattg cttaaagagt ttgctccttt aagcaacatt ttttctctag gtctatcatc
     4021 ttcactccaa tcttttatac cagccatata aaattatttg atgaagaaat aaaacctata
     4081 agcataaaaa aactcatagg tttttgtaaa gaaattataa agcattaatt attgttgtaa
     4141 actcttctgc atttaaagat gcaccaccaa ccaaacctcc atccacatca ttttgtgcaa
     4201 aaagatctgc agcattactg cctttaacgc taccaccata taaaatagaa atttgttcag
     4261 caactgtttt cccatattta ttggctaaaa cattacgaat atgttggtgc atttcttgag
     4321 cttgttcatt acttgccgtt ttccctgtac ctattgccca tattggctca taagcaatta
     4381 ctacttgcaa aatttgttct acagataaat gaaataaaga ttcttttagt tgattttcaa
     4441 caaaactatt ttgattattg gcttctcgta tttctaaagc ctcaccacaa caaaaaatag
     4501 gtgttaatgc atattctaaa gcaatgttta ctttttctgc taatgtagta ttgctttcat
     4561 taaagtattc tctacgttca ctatgcccaa taataacatg tgtaatttgc atactatgca
     4621 acatttctgc actaatttct cctgtaaaag caccactctt ttttgtgtag caattttgag
     4681 ctgctatata aaaattagct ttatttttta atttatcttt agctaacaaa agatatggag
     4741 ctggtacacc aataatgctt attttatttt ctgtggtgtt tactgttgca gtacttaatt
     4801 tagttaataa ttcttctgct tgttgtaaag tgcaattcat tttccagtta gcagcagcaa
     4861 tttgtttacg catgagtttt aaatttatta agttataagt tgttgtcaaa gatatatttc
     4921 aaaacaatta tttcagcgtc cgataattgt ttgcccttaa aatacatcca attattaata
     4981 tcttcaatta ctttagagta tttatattta tcgtttaatc cccaagaaaa attagagatg
     5041 gttttaggca acagcccatt cccaaaaaca ttgcaactaa taccgtaaac agagcctgta
     5101 tttattgcag aattaatagc aactctagta aaatctccca taattacacc acattttttt
     5161 ccagcattaa ctttttcatt tgttgcctct ttataaagaa aaatttcatt gaagttgttt
     5221 tttacattgc tattgttagt tcctgctcca atattgcacc aactaccaat aaaactatca
     5281 cctaaataac catcatgggc tttattacta taatcgctga tgatgctatt tttaatttct
     5341 ccacttgcca cagcaaattt accaatggtt gtagcaccat aaatttttgt acctgcttta
     5401 ataactgcat tttctaaaat agcacaacta cctctaataa tactaccttc cattattgta
     5461 gcatttttgc caacataaat aattccattt tgggcattta aaatacaatg ctctacatta
     5521 gcctcttcat ctataaaaat attttctgga ttaataacaa cattagtgga agaaatttta
     5581 taagattgtt tttgtttaat aatccaatca aattgctctt tcaaaatttg ttcatttatc
     5641 tgaaataaat gaaatggttg agttattcta ttgacaaaat tcctattatc tataatttta
     5701 atttttttaa atatttcaga caaagtgtta ttaaaattac tagttttaaa agcaattaat
     5761 ccaaactcat ctgcaatagc ttcagatgat tgcagagcaa gtattttgtg aatgatatta
     5821 ttatctggta ctacagatgc atcaataaat aaatgttctt cactgtcatt tatttcttca
     5881 taaagtggtt gtaaataatc atcagttttt agtagtattg gtttattgag agcctgttgc
     5941 caccactctt ttaatgtaaa aatacctaaa cgtacatcgc ctaaagattt atttagtgta
     6001 atagggtaga agttttttct tataggtgta tcaaataaaa taattgacat gatggaaggc
     6061 aagataataa aaaatgtaat tagaaagttg gattagttaa gaagtaaaaa ttattccttt
     6121 tatgaaataa tttcattaaa attttttttt tgaaaattaa tcatacagca acaaatagtt
     6181 aactgcgata gttgatttct gacaggtaaa tcaattattc tttaatttgt gcaatattca
     6241 aactagtact tgttgttaca gcttttttaa actttttcaa ctgttttgct aattttcctt
     6301 ttattcattt atgtttgctg cactctttaa aataaaaacc aatgaatttt aaaaaagtaa
     6361 ataatattac gggatggatt attgccctaa ttgcttgtac tgtttatatt gtaactgctg
     6421 aggcaggggg gagtttatgg gattgtggag aatttgtaag tagttgtttt aaacttcaag
     6481 tacctcaccc tccaggtgcc ccaatgtttg ttattttagg tagatttttt attgtacttt
     6541 tgggtaacga ccctttaact gcagcaaaag cagttaatat tatgagtgct ttggcaagcg
     6601 gttttaccat cttattttta ttttggacca ttacccactt tgcaagaaga attgtacatt
     6661 ctgatgtaag taaaccttta acccaaaccc aaattttatc tataatgggt gcaggtgttg
     6721 taggtgcttt agcttatact tttagcgata gcttttggta cagtgctgtt gaaggggaag
     6781 tgtatgctct tagttctttc tttactgctt tagttttttg ggcaattttg agatgggaac
     6841 atgaagcaga ccaaaaagga gctgaccgtt ggatagtttt tatatttttt atgatgggct
     6901 tatctattgg ggttcactta ttaaacttac ttactattcc tgcaattgta atgctgtatt
     6961 atatcagaaa aagaattgat tttaattaca aaattattcg taagtatttt atattattta
     7021 ccattattgg aggttcattg gcttttattg gtactttaat agttgccaat agcgaggcag
     7081 atagagtgcc tttagatgca actttggctg gtttagcttt agttggtaca gctttagtaa
     7141 ttgtattctt aagacttgta gaatcattta aaaaagaaaa gaaagaatac tacggaggta
     7201 tgtatatctt ttttatttta ggttgtatta taactggtgt tgtacaagtt ggggtaatac
     7261 aatatacagt taaaatggct ggtgcttttg atagatattt tgtaaatggt tttggtatgc
     7321 cattctttag tggctttgca ttcttcttta ttttattagc agcattaatt tggtatggat
     7381 taaaaattgc tgcaaaaaaa ggatggtact acttaaaatt aagtttatgg tgtgttacat
     7441 ttatgcttat tggttatagt acttatgtaa ccacaatgat tcgtagtaat gcagatcctg
     7501 ctattgatat gtacaatgta gataacccaa tgagtttagt gggttattta ggacgtgaac
     7561 aatatggcga ttttccaatt ttatatggtc aaaaatttac agcacgtcct tacgactcta
     7621 aagaaacagg tacaaaatat caaaaaggga aagacaacta tgtagaaatt ggaaaagact
     7681 ttaaatacat atatgcagca gaagataaaa tggtttttcc aagagtgtgg gatgcaagta
     7741 atgatcaatc tcatgcagat tattatgcca gctttttaaa cattggccaa atgcaagatg
     7801 gtacgtatga aagaagccct aaccaaatag ataatatgca cttcttttta gggtatcaaa
     7861 ttaatttcat gtacttcagg tattttatgt ggaatttttc tggcaaacaa aatgatattc
     7921 aaggttcttc cttagccaat ttaagagatg gtaattggat aacaggaatt aattttattg
     7981 ataacctacg tttaggtaat caagataaaa tgccagatag tttaacccat aacaaagcaa
     8041 ataataaact ttatgcttta ccttttatac tagggctaat tggtttattg tatcatttta
     8101 aacaaaaagg tgccgatgct ttagtaaata tgctattatt cttctttaca gggcttgcaa
     8161 tcgttgttta cttgaatcag gctggtaacc aaccaagaga gagagattat gcgtatgttg
     8221 gtagttttta tgcatttgct gtttggattg ggttaggtgt attgcaagta aaatcctgga
     8281 ttactcgtgt tgtaaaccaa aaagtggcag caactttggc taccgttatt tgtttattgg
     8341 cagtacctgt tttaatggct caacaagagt gggatgatca tgatagaagc aacaaaacac
     8401 ttgcaagaga tttagcaaaa gattacttag aaagttgccc taaagatgcc atactttttt
     8461 gttttggaga taatgatact tacccattat ggtatgcaca agaagtagaa ggaatacgaa
     8521 aagatattag agtaattaat tttagtttat taggtataga ttggtacatt aatcagttgc
     8581 gttataaagt aaacgaaagt gcaccaattg atgtgatttg gagtgcagac caaattgaag
     8641 gtaggaaaag agattatgta ccatttaaac caaacccgaa atttccagaa agtgcttatt
     8701 acgatttgta tgatgtaatg aaaaattata taggtagtga taatcctgca tttatagata
     8761 actcaagagg tgagccttta aatactttcc cagttaaaaa atttgctatt cctgttaata
     8821 aagatgagat aattaaaaat ggcattgtaa atgctacaga ttctatttta gcagaactta
     8881 gatttgattt aaataaagac agattattga aaaacgattt ggctgtatta aatattattg
     8941 ctgccaataa atggaaacgt cctatatgtt ttacaagcaa taacccatac caaataggct
     9001 taggttttga aagattttta aggatggatg gattaactta cagattggtg cctgtagata
     9061 acagcaatgg aacatttaat accaatgctc ctttggtaaa taccgattgg gcatacgata
     9121 aaatgatgaa taattttgct gcaggaaatg caaatgtaaa aggagtatat tttgatgaag
     9181 aaaatagaag gcatttaaac tctataagag aagcctatac agaattagca tttgatttaa
     9241 taagtaaaaa cagaatgaat gatgctaaaa aagtattaca acattgcgat tcattaatgc
     9301 tacaagagaa tgttccttat ggaatggtaa gtagagcaaa tcagcacaat cgtaatagtt
     9361 taaggttttt aactgcatgc tatgaagtag gagataaaga tttagctgca aaagtattaa
     9421 aaagcctaaa aacagatatt gagcaacaac aagtttacta tgcaagttta ggtgatggta
     9481 aaacatctgt tgaggcttat aaaaagctaa tggatgatta tattactcga tataattctg
     9541 ctcgtacaag gcaagatcag gttactgctg ataatatttt acaaagcaat ttatctcaac
     9601 gtcaaattaa ttcattaaac gatattagaa tgagctatat gctgcttgtt gaattaaata
     9661 atgtagaaat tactaacaat aaaacacaac aagttccagc acaaacaagt gtggatacaa
     9721 caaaaaaata ataaataaaa ctagtgtaaa ataaggttat gtaaaatttc aattaaattt
     9781 tatgtagcct tatttttata ttacaatatg ggtttgtttt aatacaccca ttacaaaaac
     9841 actctgcaca tttgcaatat tttcactctg gctaagtttg ttaacatgaa aatgataata
     9901 atcatccatg ctttctgcta ctacttttaa cataaagtca aactcgccag aaatattaaa
     9961 acactcaatt acttctggta aactatgaat atgtttgata aattttgagc ctgcattttt
    10021 gctatgttct tttaaactta cataacaaaa aaccattaaa ctttttttca cttttgcata
    10081 atctaataaa gtggcatatt gttttattac tccaaattgt tcaagccttt taattcttt
//
LOCUS       DAOQHP010000032        10074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3378_length_10074_cov_3.634694, whole genome shotgun sequence.
ACCESSION   DAOQHP010000032 DAOQHP010000000
VERSION     DAOQHP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_10074_cov_3.634694"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLY81_02025"
     CDS             complement(<1..653)
                     /locus_tag="PLY81_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF29037.1"
                     /translation="MFYLLQVAGNVADSVNKMNTAINTADKISMIDLLNKGGWIMYPL
                     YFLFAATIFVFTERFLAIRKAGKIDDNFMRIIRDNILSGNVTAARSMAKNTGNPVAKM
                     IDKGLQRIGKPIDAIEKSMENVGKLEIYKLERNTSILSLIAGIAPMFGFLGTIVGMIE
                     LFYNINNSGFELNTIAGGIYVKMVTSASGLIIGLIAYLAYNFLSTQIDKTVYKMEAA"
     gene            complement(793..3201)
                     /locus_tag="PLY81_02030"
     CDS             complement(793..3201)
                     /locus_tag="PLY81_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29038.1"
                     /translation="MIKKTLAACILTIAFCLISKHIFAQPDSILKVYATDFQEERIFI
                     HFDKTTYLPGETIWFKAYLASGIDLSYTSKNFYAEFTDSAGNVLQHITAPIVLSSAKA
                     MFVIPKDYASNNIFLRAYTSWMLNFDSSFIYKKNIPVIQPVNTKKFRAQKPKTSITFF
                     PEGGNLVNNIKSVVAFKANTKTGKPVKIKGLLFNNKGKILDSISSVHDGMGSFTLTPI
                     PNETYYVQWMDEWNTKYKSILPTANEEGIVMNVVQQEYDKLNVQLHRQENATNEFKQL
                     RVVATMHKNMVYRSNVKLVNNTNITVPIPIAHLPTGVLQITIFNVNWLPIAERIFFVK
                     TDNYESLVDARIFKPNTTKKAKNNLEISIPDEMLTNISVAITDAGLTESEDENIISSL
                     LLTSDIKGKVYNPLFYFSNNADSTKQFLDLVMQTHGWRKLKWMDIAQNKTPEITYPKD
                     SAYVSVAGKVFGANASQLRQAQSLNVFVKTKDSATKFLSLPLQPDGSFVQKDFIFFDT
                     VTVMYQFNKYKDLSYSTAINFSNLLLNSENKINIDSLYSYNDKEDTIGNAKKIQLWEQ
                     QKKLAKLSQSTTLDDVVVTTKTKKPEDKLDAQYATGFFQGGDAYQFDMESNRNSAAIS
                     DVLTYLTGRIAGLTITHSGNPPKITWRGGTPGLYLNEMLVDAATLQEIYVSDVAYIKV
                     IKPPFFGGMGGSGSAGAIAVYTKKGKNSNENNFKGLDKKRIAGYTAIREFYSPDYDMN
                     PELRQEKDIRTTIYWNPYVILTSQNRTVLLNFFNNDVTKKFRLSIQGMNEEGKLISIH
                     KIIE"
     gene            complement(3229..4254)
                     /locus_tag="PLY81_02035"
     CDS             complement(3229..4254)
                     /locus_tag="PLY81_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29039.1"
                     /translation="MKKIVLAVLLIFQIATVVVAQPNQTAKELHETARTFMRQGDYDN
                     ALLVIKRAAEQDPDNLEILKDLAFLYFLKRDYANAMQIGKVIIDRPDADVQSFQILGL
                     TYKAIAEYKECKKLYKKALAKFPESGVIYNEYGELLAMDNDLNNAIVQWEKGIEVDAN
                     YSSNYYNAVKYYAQNPNPELLWIALHGENFVNLESYTARTKEIKKIILEAYKKMYAYS
                     NLLENTPKKTSGFETAVLSILNNSKDIANNGINAESLMAIKARFVLDWFEGKQQKFPF
                     RLFDHYQHMLKEGIFDAYNQWLYGSVANADAYELWIKNNDKQAEAFKKFQNARVYKIP
                     QGQYYKK"
     gene            complement(4397..5635)
                     /locus_tag="PLY81_02040"
     CDS             complement(4397..5635)
                     /locus_tag="PLY81_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNF29040.1"
                     /translation="MAIIKPFKALRPQPQLAKQVASRPYDVLNSEEAKQEALGNPYSF
                     LTVTKSEITLNSNIDIHSQEVYDKAKENIETFIKHNVLFKEDKSCYYIYQLVMDGRSQ
                     TGLVVASSIDDYENGLIKKHEFTRPEKELDRINHIKTTGAQTGNVFLAYRNNVNIDTI
                     VENWKASKKPVYDFVADDQIQHSIWVVNDDNVIQNISSIFEKEIPCTYIADGHHRAAS
                     ASKVRKDTGIKHTDGGDYFLTTLFPSNQLQIMDYNRVVKDLNGLTLEQFFDLLKNNFE
                     IILTEKNQVKPSTLHEFGMYFENNWYKLIAKNGVYTNDPIGVLDVTILSNLILDAILG
                     IKDQRTDKRVDFVGGIRGLKELEKRVDSGEMKVAFSLYPVTINQLFDIADCNNVMPPK
                     STWFEPKLRDGLLTHLIYEA"
     gene            complement(5665..6735)
                     /gene="serC"
                     /locus_tag="PLY81_02045"
     CDS             complement(5665..6735)
                     /gene="serC"
                     /locus_tag="PLY81_02045"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNF29041.1"
                     /translation="MRKHNFNSGPSVLPKEVLEQASEAIINFNGINLSLLEIGHRTNW
                     FVDVIEESKLLVKELMQLNNNYEVLFLHGGATTQFMQVPLNLLNENETAAYCDNGIWG
                     SKAISEAKIFGKVNVIASSKDQNYNYIPKSFTLPDNTKYLHLTSNNTVEGTQWHQFPE
                     TTIPIVADMSSDIFSRQLNFNQFQLIYAGAQKNMGAAGVSLVVIDKNILGKVSRQISP
                     ILDYRKHIEAASLLNTPPVFAIYVALLTLRWIKKEGGLAEMENRANKKSSLLYNTIDS
                     LPLFECAIKKEDRSKMNAVFCIKEEVLQNKFLEECKEEGMIGVKGYRTVGGLRVSMYN
                     ALSLESVEAITMLMKNFANKYS"
     gene            6892..7392
                     /locus_tag="PLY81_02050"
     CDS             6892..7392
                     /locus_tag="PLY81_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNF29042.1"
                     /translation="MAKKIFYSFLAIIAVLQLFQPDRNISTENFKVELANHYSVPDSI
                     ESLLNTVCYDCHSNNTDYPWFVNIQPIGWYLQGKINKGKSKLNFSEFGSLFKEKAIEK
                     LENIEEVMKKNTMPLTSYKWYNKNADLTIEQREMISKWAINLSKQIASDSTAALNADS
                     LPVKPL"
     gene            7492..7848
                     /locus_tag="PLY81_02055"
     CDS             7492..7848
                     /locus_tag="PLY81_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29043.1"
                     /translation="MVNYINDSTNQLARYKITAECTEPFEHLIIAGFCSNVQTENILY
                     RHLQNVCKQSKGNFSNAHTLINDTITHWQNTFNLPAVQFLKNINTEKTACRYNKICLY
                     KINHQATEELLYNIYL"
     gene            complement(7870..8799)
                     /locus_tag="PLY81_02060"
     CDS             complement(7870..8799)
                     /locus_tag="PLY81_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF29044.1"
                     /translation="MTKRIFQTLVIFLSSTILVYAQKTEEQNDKKKDKESSVTKKEKE
                     EKYVLVIDGDQITVNGIPVEKYALDNVEIVRGKRFKGQENGKHFFENFRELNELFIDK
                     MKPNKAILGVSTKFEKEGAIVVNVSKESAAEKAGLQIEDIITQIDKEIVSDTNSLYKI
                     IGKYKPNDKVTITYIRNGKTQTTTAILDKYKGPNVVSLNNIYEGFDMKNNGGNFSPRR
                     PKIGLEIQDVEEGNGVKVLDVEDESPAEKAGLKEEDIITEINGKELKSVDDFKVNLKD
                     LKEGDTFKLKYKRSNKTIQTEIKLPKRLKKASL"
     gene            complement(8891..9760)
                     /locus_tag="PLY81_02065"
     CDS             complement(8891..9760)
                     /locus_tag="PLY81_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF29045.1"
                     /translation="MNPKNVLGNPTKLLLLFFSIFILSCINNNANNIPVLTSKNDSAK
                     LKESIKVIVDSNAIKFERKPVNYDSNKTYIYLTFDDGPGHGTMNCLDVCKKEGIKASF
                     FMVGKHANDSWGQQTVKTIRNAYPQFLLSNHSYTHTNEKYVYFYSHPLMAEQDFYKAQ
                     ESLNVPYKIIRLPGNNAWVTKDKIRTSKLVKPVCQKLNDAGFNVIGWDVEWNFTKGNS
                     FPVQSPQQVAAQVKNAASGQSFTKKHIVILTHDRMFRTPEYANWLSEFIKTLKQNSNF
                     VFETIDNYPNLKF"
     gene            9942..>10074
                     /locus_tag="PLY81_02070"
     CDS             9942..>10074
                     /locus_tag="PLY81_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF29046.1"
                     /translation="MSFTLASLPYGYDALEPHIDTTTMQIHHGKHHQAYVDNLNKAIA
                     "
BASE COUNT         3276 a         1618 c         1283 g         3897 t
ORIGIN      
        1 cttgcagcct ccattttata aactgtttta tctatttgtg tacttaaaaa attataggct
       61 aaataggcta ttaaaccaat aattaaacca gaggcactag ttaccatttt tacataaata
      121 ccacctgcaa ttgtatttaa ttcaaagcca ctattattaa tattataaaa caattcaatc
      181 atacctacaa tggttcctaa aaaaccaaac ataggtgcaa tacctgctat taaagataga
      241 atagatgtat ttcgttctaa tttataaatt tctagtttgc caacattttc catacttttt
      301 tcaatggcat caattggttt acctatacgt tgcaatcctt tgtcaatcat ttttgcaaca
      361 ggattaccag tattttttgc catacttctt gcagcagtaa cattaccaga taatatatta
      421 tctctaataa ttctcataaa gttatcatct attttaccag ctttacgaat agctaaaaaa
      481 cgttcggtaa aaacaaaaat ggttgcagca aataaaaagt ataaaggata cataatccaa
      541 cctcctttat ttaataaatc tatcattgaa attttatctg cagtattaat agcagtattc
      601 attttgttta cagaatctgc tacattcccg gctacttgca ataaataaaa catttgtata
      661 aattttgagg taggctaaaa tactcagcat tacaaaaaaa caaaagcctt gtgaataaaa
      721 acagaagttt gttatatttt aagatagctt ttgcacattt actccttcat gtgcatatta
      781 tctattgaat aattactcaa taattttatg aatactgatt aatttgcctt cttcattcat
      841 tccttgaata cttaatctga attttttagt aacatcatta ttaaagaagt tcagtaacac
      901 tgttctattt tgagatgtaa gaattacgta tgggttccaa taaatagttg ttcgtatgtc
      961 tttttcttgt ctcagttctg ggttcatatc ataatctggc gaataaaatt ctcttatagc
     1021 tgtatatcct gcaatccttt ttttatccag ccctttaaaa ttattttcat tactattttt
     1081 accttttttg gtataaactg caattgctcc tgcagagcca gaaccaccca taccaccaaa
     1141 gaatggaggt ttaattactt taatataagc cacatcgcta acataaattt cttgcaaagt
     1201 agcagcatct accaacatct catttaaata caaaccaggt gtgccaccac gccatgtaat
     1261 ttttggtgga ttaccagaat gagttatagt aagccctgca attcttcctg ttaaataagt
     1321 taatacatct gatattgcag cactatttct gttactttcc atatcaaatt gatatgcatc
     1381 accgccttga aaaaagcctg tagcatattg tgcatctaat ttatcttcag gcttcttagt
     1441 ttttgtagta accactacat catctaaagt tgttgactgg cttagttttg ctaatttttt
     1501 ctgctgctcc cataattgta ttttttttgc attaccaatt gtgtcttctt tatcattata
     1561 actatacaag ctatctatat taattttatt ttctgaattt agtaataaat tagaaaagtt
     1621 aattgcagta gaatacgata aatctttgta tttattaaat tgatacatga cagttacggt
     1681 atcaaaaaat ataaaatctt tttgaacaaa gctgccatct ggttgtaaag gaagggataa
     1741 aaatttagta gccgaatctt ttgtttttac aaatacattt aagctttgtg cttgccttaa
     1801 ttgagatgca tttgcaccaa acactttacc agcaaccgat acataagcag agtctttagg
     1861 gtaagtaatt tctggtgttt tattttgagc tatatccatc cattttaatt ttctccaacc
     1921 atgtgtttgc attactaaat ctaaaaactg tttagtgcta tctgcattgt ttgaaaaata
     1981 aaataatggg ttataaactt tacctttaat atcgcttgta agtagtaaag aggagataat
     2041 attttcatct tcactttctg ttaatcctgc atctgtaatg gctacagata tattggtaag
     2101 catttcatct ggtatgctaa tttctaaatt attttttgcc ttttttgtag tgtttggttt
     2161 aaaaattcta gcatctacta aagattcata attatctgtt ttaacaaaaa aaattctttc
     2221 ggctataggt aaccaattta cattaaatat agttatttgt aaaacccctg ttggtaaatg
     2281 agctattgga attggaacag taatattggt attgtttacc aatttaacat tgcttctgta
     2341 caccatattt ttatgcatgg ttgcaactac tcttaattgc ttaaactcat ttgttgcatt
     2401 ttcttggcga tgtaattgta catttagttt atcatactct tgttgtacca cattcattac
     2461 aattccttct tcatttgcag taggtaaaat tgatttgtac tttgtattcc attcatccat
     2521 ccattgtaca tagtaagttt cattgggtat tggagttaat gtaaaactgc ccataccatc
     2581 gtgtacagag gaaattgaat ctaaaatttt acctttatta ttaaacaata acccttttat
     2641 ttttacaggc ttacctgttt ttgtatttgc tttaaaggct actacacttt taatattgtt
     2701 tactaaattt cctccttcag gaaaaaaagt aattgaagtt tttggctttt gtgctctgaa
     2761 tttttttgta tttacaggct gaattactgg aatatttttt ttataaataa aactgctgtc
     2821 aaagtttagc atccagctag tatatgctct taaaaaaata ttgttagaag cataatcttt
     2881 aggaataaca aacattgctt ttgccgatga taaaacaatt ggtgccgtaa tatgttgtaa
     2941 aacatttcca gcactatcag taaattcagc ataaaaattt ttactggtat aacttaaatc
     3001 aataccacta gctaaataag ctttaaacca aatagtttcg cctggcaagt atgtagtttt
     3061 atcaaaatgt ataaatattc tttcttcttg aaaatcggta gcatacactt ttaaaataga
     3121 atcgggctgt gcaaatatgt gtttgcttat taaacaaaaa gcaatagtta aaatgcaggc
     3181 agctaatgtt tttttaatca tagctactaa tggttaaaat ataaagaatt attttttgta
     3241 gtattgacct tgtggaattt tatatactct tgcattctga aattttttaa atgcttctgc
     3301 ttgtttatca ttatttttta tccaaagttc ataagcatct gcattggcaa cagagccata
     3361 aagccattga ttatatgcat caaaaattcc ttcctttagc atgtgttgat aatggtcaaa
     3421 taatctaaat ggaaattttt gttgtttgcc ttcaaaccaa tctaaaacaa atcttgcttt
     3481 aatagccatt aaactttcag cattaattcc gttattggct atgtctttac tattgtttaa
     3541 gatagataat acagcagttt caaatccact ggttttttta ggagtattct ctaataagtt
     3601 actataagca tacatttttt tatacgcctc taaaataatt tttttaattt cttttgttct
     3661 agcagtatag ctttctaggt taacaaagtt ttctccatgt aaagcaatcc acagtagttc
     3721 tggatttggg ttttgtgcat aatactttac tgcattataa taattactac tataattggc
     3781 atctacttca attccttttt cccattgaac aatggcatta tttaaatcat tatccattgc
     3841 taatagttcg ccatattcat tataaattac accactttca ggaaattttg ccaatgcttt
     3901 tttatataat tttttacact ctttatattc agcaatagcc ttgtaggtta ggccaagtat
     3961 ttgaaaagat tgaacatcag catctggtct atcaataata acttttccta tttgcattgc
     4021 attggcataa tctcttttta agaagtatag aaatgccagg tcttttaaaa tttctaaatt
     4081 atctgggtct tgttcggcag ctctttttat aaccagtaat gcattgtcat aatctccttg
     4141 tcgcataaat gttctagctg tttcgtgcaa ttcctttgca gtttgatttg gttgagcaac
     4201 cactaccgtt gcaatttgaa aaattaataa aacagctaaa actatttttt tcatgtataa
     4261 tattattttg gcctttggta ttgtttagtt gaattacaac ttattattta attattaaaa
     4321 tatttgctct actaaatttt tggttgaaat ttatagaggt gagataatat tttaagaagt
     4381 ataaataaat tgttttttaa gcctcataaa ttaaatgtgt tagtaaccca tctcttaatt
     4441 ttggctcaaa ccaagtactt tttggtggca ttacattgtt gcaatctgca atatcaaata
     4501 actgatttat agttacagga tataaactaa aagcaacttt catttcacca ctatctactc
     4561 ttttttctaa ctcttttagc ccccttattc caccaacaaa atcaacacgt ttatcggtgc
     4621 gttgatcttt aattcctaaa atagcatcta aaattaagtt agataaaatg gttacatcta
     4681 aaacacctat tgggtcatta gtataaactc cattttttgc tataagttta taccaattgt
     4741 tttcgaagta cataccaaat tcgtgtaaag ttgagggctt tacttgattt ttttccgtta
     4801 aaattatttc aaaattattt tttaacaaat cgaaaaattg ttctaaagtt aatccgttca
     4861 aatcttttac aacacgatta taatccataa tttgtaattg attagaagga aataaagtgg
     4921 ttaaaaaata atctccccca tctgtatgtt ttatacctgt atcttttctt actttagagg
     4981 cagatgcagc tctgtgatgc ccatctgcta tatatgtaca tggaatttct ttctcaaaaa
     5041 ttgatgaaat attttgaata acattatcat catttacaac ccaaatagaa tgttgaattt
     5101 gatcatctgc aacaaaatca tacactggct ttttagaagc tttccagttt tctacaatag
     5161 tatctatatt aacattattt ctataagcta aaaaaacatt cccagtttgg gctcctgtag
     5221 ttttaatatg gtttattcta tctaattctt tttctggcct tgtaaattca tgttttttta
     5281 ttagcccatt ttcataatca tcaatactac ttgctaccac caaacctgtt tggcttctac
     5341 catccataac taattgataa atgtaataac agcttttatc ttctttaaat aaaacattat
     5401 gttttataaa tgtttctata ttttcttttg ctttatcata tacttcttga gagtgtatat
     5461 ctatattact gtttaaggta atttcacttt ttgttactgt taaaaacgaa taaggattac
     5521 ctaaagcttc ttgctttgct tcttcactat tcaacacatc gtatggacgg ctagctactt
     5581 gttttgctaa ttgtggttgt ggtcttaaag ctttaaatgg tttaataata gccatgttct
     5641 gattttttat ttattgaatt tattttatga atatttattt gcgaaatttt tcattaacat
     5701 tgttattgct tctacacttt caagtgatag tgcattatac atgcttactc ttaatcctcc
     5761 tactgttcta taccctttta ctccaatcat tccttcttct ttacattctt ctaaaaattt
     5821 attttgtaaa acttcctctt ttatacaaaa tacagcattc attttactcc tatcttcttt
     5881 tttaattgca cattcaaata atggtaaact atcaatggta ttgtataata aacttgattt
     5941 tttgtttgct ctattttcca tttctgctaa tccgccttct tttttaatcc aacgcaatgt
     6001 aagcaaagca acataaatag caaataccgg gggagtattt aataaacttg cagcttctat
     6061 atgttttcta taatctaaaa taggagaaat ttggcgactt acttttccta aaatattttt
     6121 atctattact actaaactaa caccagcagc acccatattt ttttgtgcac cagcataaat
     6181 taattgaaac tgattaaaat ttaattgcct actgaaaata tcgctgctca tatcagcaac
     6241 aataggtatg gtagtttcag gaaactggtg ccattgtgtt ccttcaacgg tattgttgct
     6301 tgttaaatgc aaatattttg tattatctgg taaggtgaag cttttaggaa tataattata
     6361 gttttgatct tttgaagaag caataacatt aaccttacca aaaatttttg cttcacttat
     6421 tgctttgctt ccccaaatgc cattatcgca atatgcagca gtttcatttt catttaataa
     6481 atttaatgga acctgcataa actgtgtagt ggcaccacca tgtaaaaaaa gcacttcata
     6541 attgttgttt aactgcatca attctttaac caacaattta gattcttcta ttacatctac
     6601 aaaccaattt gttctatgac caatttctaa taaagaaagg tttataccat taaaatttat
     6661 tattgcttct gaggcctgtt ctaacacttc tttgggtaaa acagatggac cagagttaaa
     6721 attatgtttc ctcatttcgg cacaaacata aaataaatca ataagtattt tatgttttta
     6781 atttattaga cttttattaa taaaaataaa gcaatctgaa atttgtaaag taatctacaa
     6841 atgttaaaaa atgttttttg gtaaactaat cttttctccg aaatttattg cgtggcaaaa
     6901 aaaatatttt actctttttt agcaataatt gctgttctac aattgtttca accagataga
     6961 aatatatcta ctgaaaattt taaagtagaa ttagccaatc attactctgt accagatagt
     7021 attgaatctc ttttaaatac tgtttgctac gactgtcata gtaacaatac agattatcct
     7081 tggtttgtaa atattcaacc tattggttgg tatttgcaag gaaagataaa taagggcaaa
     7141 agcaaactta atttttctga atttggaagt ttatttaaag aaaaagcgat agaaaagtta
     7201 gaaaatatag aagaggtaat gaaaaaaaat actatgccat taacctctta taaatggtac
     7261 aataaaaatg ccgatttaac tattgaacaa agagaaatga tatctaaatg ggctattaat
     7321 ttaagtaagc aaattgcaag cgatagtact gcagctttaa atgcagattc cttacctgtt
     7381 aaaccacttt aattaaaaat tttaaaatta aattcccaaa aaaataatag aattcactaa
     7441 attactaatt tttttagtat atttgaacta caaattttag gaatttattt tatggtaaat
     7501 tatattaatg atagtacaaa tcaactagcc agatataaaa taactgcaga atgcacagag
     7561 ccatttgaac accttattat tgcaggtttt tgctctaatg tgcaaacaga aaatatatta
     7621 tacaggcacc tacaaaatgt ttgtaaacaa agcaaaggaa attttagtaa tgcccatacg
     7681 ctaattaatg atacaataac tcactggcaa aatactttta acctgcctgc ggtacagttt
     7741 cttaaaaata tcaatacaga aaaaacagct tgcagatata ataaaatttg cctttataaa
     7801 attaaccatc aagcaaccga agaactattg tataatattt atttatagta ataagcaata
     7861 actaaaaaat tacaaacttg ccttttttaa tcttttaggt aatttaattt cagtttgtat
     7921 ggttttattg cttcttttat actttagttt aaacgtatct ccttctttaa ggtcttttaa
     7981 atttacttta aaatcatcca cactcttcaa ttcttttcca tttatttctg taataatatc
     8041 ctcttctttt aaccctgctt tttctgctgg tgattcatcc tctacatcta atacttttac
     8101 accattgcct tcttccacat cctgaatttc taaaccaatt ttggggcgac gaggcgagaa
     8161 attgccaccg ttatttttca tatcaaaccc ttcgtaaata ttatttaatg atactacatt
     8221 agggcctttg tatttatcta gtatggcagt tgttgtttgt gttttaccat ttctaatata
     8281 agttatagtt actttatcat taggtttata ttttccaata attttatata aactattagt
     8341 atcactcaca atttctttat ctatttgggt tattatatct tctatttgta aacctgcttt
     8401 ttctgctgca ctttctttac ttacattaac aacaattgca ccctcctttt cgaatttagt
     8461 tgaaacgcca aggattgctt tattaggttt cattttatcg ataaatagtt catttaattc
     8521 tctgaaattt tcaaaaaagt gcttgccatt ttcttgtcct ttaaatcttt tacctctaac
     8581 tatttcaaca ttatccaaag catatttttc aacaggaatg ccatttacag taatttggtc
     8641 cccatctata actaaaacat atttctcttc tttttctttt ttagtaacag aagactcttt
     8701 atcctttttc ttatcatttt gctcctcagt tttttgagca taaactaaaa tagtagaaga
     8761 taaaaaaata actaatgttt gaaaaattct ttttgtcata acagaataat ttaatgatgt
     8821 acgaatattt tagtagaaca aatataaaaa agaatccgat atacgaaaca tttcggaaat
     8881 gttaaatatt ttaaaatttg aggttggggt aattatctat tgtttcaaat acaaaatttg
     8941 aattttgttt taatgtttta ataaattcac tcaaccaatt ggcatattct ggagttctaa
     9001 acattctatc atgcgttaaa ataactatat gctttttagt aaagctttgc ccactggctg
     9061 catttttaac ttgtgctgca acttgttgag ggctttgaac aggaaaagaa ttacctttgg
     9121 taaagttcca ttctacatcc catccaatta cattaaaacc agcatcattt aatttttggc
     9181 aaactggttt tactaattta ctagtcctaa ttttatcttt tgtaacccaa gcattgttac
     9241 ctggtaatct tattatttta taaggcacat ttaaactttc ttgtgcttta taaaaatctt
     9301 gctcggccat tagtggatga gaataaaaat aaacgtattt ctcgttagta tgcgtatagc
     9361 tatgattgga taatagaaat tgtgggtatg catttctaat tgtttttaca gtttgttgcc
     9421 cccaactatc atttgcatgt ttacccacca taaaaaagct tgcctttatt ccttcttttt
     9481 tgcatacatc caagcaattc attgtaccat gccctggccc atcatcaaat gttaaataaa
     9541 tgtatgtttt atttgaatcg taattgacag gtttcctttc aaactttatt gcattggagt
     9601 caacaataac cttaattgat tcttttaatt ttgcactatc atttttactt gtaagaacag
     9661 gaatattatt tgcattatta tttatgcaag agagaataaa aatggagaaa aataatagta
     9721 ataatttggt tgggttgcct aacacatttt ttgggttcat tgatactatt ttgtaactaa
     9781 attatacaaa aaatatatag gagctagcta aatgattgtt ataatcttaa tttgcttact
     9841 tacgacaatt ggaagaattt attgaactat taatatagta agcataatta attaaatttg
     9901 tggtattcat ttgtaattat tcattattaa aattaaatat tatgtctttt actttagctt
     9961 ctttacctta tggttacgat gccctagaac cacacataga tacaacaact atgcaaatac
    10021 atcatggcaa gcaccatcag gcttatgtag ataatttaaa caaagccatt gctg
//
LOCUS       DAOQHP010000033        10015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3417_length_10015_cov_3.814960, whole genome shotgun sequence.
ACCESSION   DAOQHP010000033 DAOQHP010000000
VERSION     DAOQHP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_10015_cov_3.814960"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLY81_02075"
     CDS             complement(<1..343)
                     /locus_tag="PLY81_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29047.1"
                     /translation="MPKLIILFFVSAVLFACNQNTSIEKKFDEDNYETTKETLAEKEK
                     KNPARFLQVENRERKNIIGQTVVIGHFTNTATVCTYKDVQIKLSFFSKTGTQVDEGME
                     TIYETIAPGKRV"
     gene            547..1203
                     /locus_tag="PLY81_02080"
     CDS             547..1203
                     /locus_tag="PLY81_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29048.1"
                     /translation="MKFYNLIIRYRFWLSLVAIVGGVILNVTGTAGFWPTFPLYFIGA
                     IGLFSNFFIGPLRLIQEPMESGNVEEVKKILDTIWFPNLLFKPVRSTYYTIKGNLAMM
                     EQDFDTAEKHLKKSSDIGSPMPEAEGANKLQLGMMAMQRGDLKQGESYIRAAIRAGIP
                     DKESEAVAYLQMCQIFMNKREFRAAKDFFRKSKACKPKTQQVLDQIKEIEKYISRMPG
                     "
     gene            complement(1507..2181)
                     /gene="trmD"
                     /locus_tag="PLY81_02085"
     CDS             complement(1507..2181)
                     /gene="trmD"
                     /locus_tag="PLY81_02085"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNF29049.1"
                     /translation="MNIDIISVAPELLDSPFAHSIMKRAKEKKLLQIQVHNLRNWAIN
                     KHGQIDDYQYGGGAGMVMMCEPLVNAIEALQQQKKFDEIIFMTPDGEPFTQQKANQLS
                     LKENILIICGHYKGIDERIREHYVTLEISIGDYVLSGGELAAAVVVDAIGRIIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPANFKGWQVPDVLLQGDTKKIEDWRLEQSIIRTEKK
                     RPNLLK"
     gene            complement(2200..2649)
                     /locus_tag="PLY81_02090"
     CDS             complement(2200..2649)
                     /locus_tag="PLY81_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF29050.1"
                     /translation="MKIKFSIFIICMVAIFKVNAQEENPDKKNEKADSTYSIVQIPAS
                     FTGGAPAWRKYLQNNLDVSLGTKYIQIPKGQKSAKVTVIVSFIIDKEGKVSEVKLHDA
                     EPDHIPYIFIKEAIRIIQTGPKWVPAVQYGKLVKYRMLQPISWVVSE"
     gene            complement(2639..3037)
                     /locus_tag="PLY81_02095"
     CDS             complement(2639..3037)
                     /locus_tag="PLY81_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNF29051.1"
                     /translation="MSSEKTRIDKYLWAIRLFKTRSLATEACDKGRVKLNGVTVKAAK
                     TVHLNEEYEIKTEARKWVIKVNGLLNQRVAYTEAIKFYTDITPPEEKATIEFKPVSFH
                     TGKILSKIGRPTKKQRRDIDNFINFDEDEN"
     gene            3103..3621
                     /locus_tag="PLY81_02100"
     CDS             3103..3621
                     /locus_tag="PLY81_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNF29052.1"
                     /translation="MISFLKISMFYLLIPAIIITSTYNNYINKIKQPTTINCKVIGIK
                     DGDTFEVLFNNKKLVVRLAHIDCPERKQPFYQNAKQYASSLCFGKNVTLVHNNNFDRN
                     KRLIAEVYIGTLNINKNLVKSGLAWHFKKYSTNTEYAIIEMEAKRNKIGIWSENNPIA
                     PWEWRKAKKKNY"
     gene            3708..6329
                     /gene="alaS"
                     /locus_tag="PLY81_02105"
     CDS             3708..6329
                     /gene="alaS"
                     /locus_tag="PLY81_02105"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNF29053.1"
                     /translation="MTSAEIRQHFLNFFESKKHEIVASAPIVVKDDPTLLFTNAGMNQ
                     FKDYFLGNKEPKNKRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYFK
                     TEAIAWSWELLTEIYKLDKDRLYVTIFEGDEKEGLPKDNEAYNEWKKVIAEDRILLGN
                     KKDNFWEMGDTGPCGPCTEIHVDCRMEEERKLVDGKLLVNNDHPQVIEIWNNVFIQFN
                     RKKDGSLEQLPAKHVDTGMGFERLVRVIQKKSSNYDTDVFTKTIAAVENITEKKYDFG
                     DNKQAIAFRVLADHIRAISFTIADGQLPSNTGAGYVIRRILRRAVRYYYSYLEYQQPL
                     LFQLVPVISTQFQNVFPELYKQKDFVSKVVKEEEEAFLRTLNKGLKLIDEIIVAANAN
                     QLKSINGNDAFELYDTFGFPIDLTRLIAAENNLSIDEEGFEIALQQQKKRSRAATIID
                     TEDWIIINEGNSVFVGYNQLSIETKILKYRKVKAKGKESYQIVLAETPFYAESGGQVG
                     DKGWIDFGNCTIEVFDIKKENNLSIHFVTTLPESINLTGNIIATVNKDLRKETANNHT
                     ATHLLHAALRNVLGSHVAQKGSLVNHENLRFDFSHFAKVTDDEIAAIEKIVNTKIREN
                     IPVIIKEMKKEEAVALGAMALFGEKYGDVVRVVIIDPNYSVELCGGTHVTNTGELGFF
                     KIKHETAVSAGVRRIEALSGAGVEEFINNQLLQLQAVKNLFNNPKELLKVIENNAIEN
                     KELKKKIESFEAKQIVALKAELNEKVEIINNTKFIGAIVDVNSADNLKKLVIELKQNA
                     DVAILATNIIGKSNVVVMINEAWENTKSLDATKIIKEHIAPVIKGGGGGSKNIATAGG
                     QEASNLPKVIDVVKALL"
     gene            6375..7190
                     /locus_tag="PLY81_02110"
     CDS             6375..7190
                     /locus_tag="PLY81_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF29054.1"
                     /translation="MQFNKKKAFRFIKVFIILYGGIGLIIYFFQDNFLFHPKKIAKDY
                     VYTFSTPFKEINIQYDSSTLFNTIQFLPKDTQAVKGVVLYFHGNRENINRYAPYVANF
                     TKNGYEVWITDYPTFGKSTGTLSEKVLETQALEIYKIAETKFKDSSIILYGKSLGTGL
                     AAYVASRKNCKQLILETPYSSITSLMKRYCFIYPIEKMIRYKLPVIEYIKKATAPIII
                     FQASEDWVIPNSNTEMLKVNLKATDKFVNIEGASHNTINNYPQYHKTLDSLLQ"
     gene            complement(7191..8330)
                     /locus_tag="PLY81_02115"
     CDS             complement(7191..8330)
                     /locus_tag="PLY81_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNF29055.1"
                     /translation="MKVGIIGGGVAGLSTALALKKANISFHLFEQASAFKDIGAGVML
                     SSSTKYLLHQLGVGNDFEKASTLIKDFNITNHHLQTIKRIPSNEIGYGIHRARLIEVL
                     SKPLQQEDYTLNARIEKIEQNESGVTIDANNTTYFFDIVVAADGVHSVTRNKFSPHIQ
                     PSYTGQIMWRGIATLNLPDKFKNAAHEMWGKNKRFNIIHKGGDDYFWFCIRWIKDGIF
                     YEEENLKQFLHQEFNDFPEYAHAVIDADNNIIKTPLRHIKFNNLNNWYNNRIVFVGDC
                     IHACTPDIAQGACQAIESAYTLAACLKKYPNNFKDAFELYQFKRKPKTKFINNASFAF
                     SRFSHQRKKWQYNVLFSVFKLLPNSFLQKKFNKTNDIAYMNDLQL"
     gene            8491..9513
                     /locus_tag="PLY81_02120"
     CDS             8491..9513
                     /locus_tag="PLY81_02120"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNF29056.1"
                     /translation="MKSLLLLTLICFIYNGKAQGLYPTYVNENVVYIDSTGSKKLETK
                     HKRCGLYQNGLAWVIINEAIGFMNTKGEVIIEPKYSETSGFYEGFASVVVDNAWGVIN
                     NKGETIVEPKYYEIGIYSDGMCKFKENKSLFSEWGFLDTKGKVAIRPTFENIDDFHEG
                     LAMFITDAKAGYIDTTGKVVIEPSFYYGAFFKINGKVVFEKGAFSNGLAAVEINNKFG
                     FIDKTGKVIIAAQYDDVKNFSEGLAAVKIKENWGFIDTKGSIIITPTYANVESFSEGL
                     AAVKIKENWGFINTKGKLIIPATLFGSIDIGIDLKFKDGVCQAMLTDKVWGYINKQGK
                     TLWKSK"
     gene            complement(9536..>10015)
                     /locus_tag="PLY81_02125"
     CDS             complement(9536..>10015)
                     /locus_tag="PLY81_02125"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HNF29057.1"
                     /translation="YFATYQNLYFSGDGALKDKDGNYRITGRVDDVLNISGHRIGTAE
                     VENAINMHVGVVESAVVGYPHAIKGQGIYAFVIVSSKHSDDELTRKDILQTVTRVIGA
                     IAKPDKIQFVAGLPKTRSGKIMRRILRKIAEGEVENLGDTTTLLDPNIVDEIKNGRI"
BASE COUNT         3585 a         1458 c         1562 g         3410 t
ORIGIN      
        1 ttactctttt acctggggca atggtttcat aaatagtttc cattccttca tctacctgtg
       61 taccagtttt actaaaaaaa ctaagtttta tttgtacatc tttatatgtg caaactgttg
      121 ctgtatttgt aaaatggcca ataacaactg tttgcccaat aatatttttt ctttctcggt
      181 tttccacttg taaaaatctg gcagggttct ttttctcttt ttctgctaaa gtttctttgg
      241 ttgtttcata attatcttca tcaaattttt tttctataga tgtattctga ttacaagcaa
      301 acaacacggc acttacaaaa aaaagaatta ttagttttgg catgagttta tttttaattt
      361 ttatataaag ctgcaataaa ttagtttttt attttttgca aagttttatg cttcctagtt
      421 ttctaagcat aaatttattc aataataagt taaataaact ttattaaaaa aagcaatggt
      481 ataaaataat ggttatgaat ctttaaaagt taactttgcc ccctttaata aatttttctt
      541 aaaaatatga agttttataa tttaattatt aggtatcgtt tttggttaag tttagtagcc
      601 attgtaggag gagttatttt aaacgtaact ggcacagcag gcttttggcc tacatttcca
      661 ctttatttta ttggtgctat tggtttattt agtaactttt ttattggccc attaagatta
      721 attcaagagc caatggaatc aggaaatgtt gaggaggtta agaaaatttt agataccatt
      781 tggtttccta atttattatt taaacctgta cgttctacct attacactat taaaggtaat
      841 ttggcaatga tggagcaaga ctttgatact gccgaaaaac atttgaaaaa aagtagtgat
      901 attggtagcc caatgccaga agcagaagga gctaataaat tacaattagg catgatggct
      961 atgcaacgtg gcgatttaaa acaaggcgaa agttatataa gagcagcaat tagagctggc
     1021 ataccagata aagaaagtga agctgttgct tatttacaaa tgtgccaaat atttatgaac
     1081 aagagagagt ttagagctgc taaagatttt tttagaaaat ctaaagcttg taaaccaaaa
     1141 acccagcagg ttttagacca aataaaagaa attgaaaaat atatttcaag aatgcctggt
     1201 taattaacta attaaaacaa aaaatcccca aaattttggg gattttttgt tttgcatttt
     1261 aatgctttta gaccggtgtt ggttctacat aggattttta agttatagag aaatcttgga
     1321 ttttaaacag gaacttttgt tttcataaag ctcaccggat tttcttttgt tgattaaaag
     1381 tagtaccaac ttttaaataa atgttaatgt attagatttt gtaatacccc aaaaagacaa
     1441 atttttcatt cagtatcaat ttaatatccc taaaagttta tgcattaacc aaaaaggatt
     1501 aattaatcat tttaaaaggt ttgggcgttt tttttctgtt ctaataatgg attgctctaa
     1561 acgccaatcc tcaatttttt tggtatcgcc ttgtaataaa acatctggta cctgccaacc
     1621 tttaaaatta gctggcctgg tataaacagg tggtgcaagt aaattatctt gaaaagaatc
     1681 agttaatgca gaagtttcat catttaaaac accaggtatt attcttccaa tagcatctac
     1741 aactactgca gcagccaact ctccaccact taacacataa tcaccaatag aaatttctag
     1801 tgttacataa tgttccctta ttctttcatc aattccttta tagtggccac aaataattaa
     1861 tatattttct tttaacgata attgattggc tttttgttga gtaaaaggtt ccccatctgg
     1921 tgtcataaaa attatttcat caaatttttt ttgttgttgt aaagcctcta ttgcatttac
     1981 taaaggctca cacatcatta ccatacctgc accaccacca tattggtaat catcaatctg
     2041 gccatgtttg ttgatagccc agtttcttaa attatgtact tgaatttgta gtaatttttt
     2101 ttcttttgct cttttcatta tgctatgagc aaaagggctg tctaataatt ctggagctac
     2161 agaaattata tcaatattca tattgcaaat ataaacctac tattcactta ctacccagct
     2221 aattggttgt aacattctat attttacaag tttgccatat tgaactgcag gtacccattt
     2281 tggcccagtt tgaattattc ttatggcttc tttaataaaa atataaggta tatggtccgg
     2341 ctctgcatca tgcaatttta cttcagatac cttaccctct ttatcaataa taaaacttac
     2401 tataacagtt acctttgctg atttttgccc cttaggtatt tggatatact ttgtacccaa
     2461 gctaacatct aaattatttt gcaaatattt acgccatgca ggtgctcctc ctgtaaaact
     2521 agcaggtatt tgcactatac tgtaggtaga gtctgctttt tcatttttct tatcggggtt
     2581 ttcttcttga gcatttactt taaaaatagc aaccatgcat attataaata ttgaaaattt
     2641 aattttcatc ttcatcaaaa tttataaagt tatcaatatc tcttctttgc tttttagttg
     2701 gccttcctat tttgcttaaa attttacctg tatgaaaaga aacaggttta aactctatgg
     2761 ttgccttctc ttctggtggt gtaatatcag tataaaattt aattgcttcg gtataagcaa
     2821 cacgctggtt taataagccg tttactttta ttacccattt ccttgcttct gttttaattt
     2881 catactcttc attcaagtgt actgttttag cagcttttac agtaactcca ttcaatttta
     2941 ctctgccttt atcacaagct tcggtagcta aacttcttgt tttaaaaagc cttattgccc
     3001 aaaggtattt atctattctt gtcttttctg aactcataat ttctcaaaaa ataatacttt
     3061 atatttaact tcttttttaa aaattgtagt ggaaaaatac aaatgatttc ttttctaaaa
     3121 ataagcatgt tttatttatt aataccagca atcattatta ctagtacgta taataattac
     3181 atcaacaaaa taaaacagcc aacaactatt aattgtaaag taataggaat taaagacgga
     3241 gacacttttg aagtattgtt taataataaa aaattagtag taagacttgc acatattgat
     3301 tgccctgaaa ggaaacagcc attctatcaa aatgccaaac aatatgcctc aagtttatgc
     3361 tttggaaaaa atgtaactct ggttcataat aataattttg atagaaataa aagacttatt
     3421 gcagaagtgt atattggtac tttaaatata aataaaaatt tagtgaagag tggacttgca
     3481 tggcatttta aaaaatactc taccaataca gaatatgcca ttattgaaat ggaggctaaa
     3541 agaaacaaaa ttggtatttg gagtgaaaac aacccaattg caccttggga atggagaaag
     3601 gctaaaaaga aaaattacta aataagcctt ttaaattatt gaataaacta aacatataaa
     3661 agcctcggcc ttatctttgc tcacttttta atacctactt ttatatgatg acttcggctg
     3721 aaattagaca acatttttta aatttttttg aaagtaaaaa gcatgaaata gttgcttctg
     3781 caccaattgt tgttaaagat gacccaactt tattatttac caatgctggt atgaaccagt
     3841 ttaaagatta ttttttaggt aataaagaac ctaaaaacaa acgcattgca gatactcaaa
     3901 aatgcttacg agtaagcggt aaacataatg atttggaaga agtaggtgtt gacacctatc
     3961 atcatactat gtttgaaatg ctaggcaact ggagttttgg agattatttt aaaacagaag
     4021 cgattgcctg gagttgggag ctacttacag aaatatataa attagataaa gaccgtttgt
     4081 atgttaccat ttttgaagga gatgaaaaag aaggactgcc aaaagataac gaagcttaca
     4141 atgaatggaa aaaagtaatt gcagaagaca gaattttgtt aggaaacaaa aaagataatt
     4201 tttgggaaat gggagataca ggaccatgtg gcccttgtac tgaaatacat gttgattgta
     4261 gaatggagga agaaagaaaa ttagtggatg ggaaattatt agtaaacaac gaccacccac
     4321 aagtaataga aatatggaac aacgtattta tacaatttaa cagaaaaaaa gatggcagtt
     4381 tagaacagtt acctgcaaag catgtagata caggaatggg ctttgaaaga ttagtaaggg
     4441 ttattcaaaa aaaatcatct aattatgata ccgatgtttt taccaaaacc attgctgctg
     4501 tagaaaatat aacagaaaaa aaatacgatt ttggagataa taaacaagct attgcttttc
     4561 gtgtattagc agatcatatt agggcaatta gttttaccat tgccgatgga cagttaccaa
     4621 gcaatactgg agcaggatat gtaattagaa gaatattaag acgtgccgtt cgttattatt
     4681 attcttattt agagtatcaa cagcctttac tttttcaact tgttccagtt atatctacac
     4741 aatttcaaaa tgtttttcct gaattataca aacaaaaaga ctttgtaagt aaggttgtaa
     4801 aagaagaaga agaagctttt ttaagaacct taaataaagg cttaaaactt attgatgaaa
     4861 ttattgtagc agctaatgca aaccaactaa aatcaattaa tggcaacgat gcttttgagt
     4921 tgtacgatac ctttggtttt cctatagacc taacaagatt aattgctgca gaaaataatt
     4981 tatctattga tgaagaaggt tttgaaattg cattacaaca acagaaaaaa agatctagag
     5041 cagccactat tatagataca gaagattgga taataattaa tgaaggaaac tctgtttttg
     5101 ttggctacaa tcagttatca atagaaacta aaatattaaa gtacagaaaa gtaaaagcaa
     5161 aaggaaaaga aagttatcaa atagttttag cagaaacacc tttttatgca gagagtggag
     5221 gccaagtagg cgataaaggt tggatagatt ttggaaattg cacaatagaa gtttttgata
     5281 taaaaaaaga aaacaactta tccattcact ttgtaacaac cctaccagaa agcataaatt
     5341 taacaggtaa tattattgct acggtaaata aagatttaag aaaagaaaca gccaataacc
     5401 atactgccac acacttatta catgcagctt taagaaatgt attaggtagc catgtggctc
     5461 aaaaaggtag cttagtaaat catgaaaact tacgtttcga tttttctcac tttgcaaaag
     5521 ttactgatga tgaaattgca gccattgaga aaattgttaa cacaaaaatt agagaaaata
     5581 ttcctgttat tataaaagaa atgaaaaaag aagaggctgt tgcacttggt gctatggctt
     5641 tatttggaga aaaatatggc gatgtagtaa gagtagtaat tatagatccc aattattctg
     5701 tagaactatg tggaggaacc catgtaacca atacaggaga actaggcttt tttaaaatta
     5761 aacatgaaac tgctgtttct gcaggcgtac gcagaataga agccttaagt ggtgcaggtg
     5821 tggaggagtt tattaataac caacttttac aattacaagc tgtaaaaaat ttatttaata
     5881 accctaaaga attattaaaa gttattgaaa acaatgctat agaaaataaa gagctaaaaa
     5941 agaaaataga aagctttgaa gcaaaacaaa ttgtagcatt aaaagcagag cttaatgaga
     6001 aagtagaaat tataaacaat actaaattta ttggtgcaat tgtggatgta aacagtgctg
     6061 ataatttaaa aaaattagtt attgagttaa agcaaaatgc agatgtagca atacttgcta
     6121 ccaatattat aggaaaatca aatgtggtag taatgataaa cgaagcttgg gaaaatacta
     6181 aatcattaga tgctacaaaa attattaaag aacatatagc acctgttatt aaaggtggtg
     6241 gtggtggctc taaaaatatt gcaacagcag gtgggcaaga agcaagtaac ttacctaaag
     6301 taatagatgt tgtgaaggct ttgctataaa ttaaaatttt aaaataccat acactatttc
     6361 ttaattaaaa taccatgcaa ttcaacaaaa agaaagcttt tcgttttata aaagttttta
     6421 ttattttata tggtggtata ggtttaatta tttatttctt tcaagataat tttttgtttc
     6481 atccaaaaaa aatagctaaa gattatgtat atactttttc cactcctttt aaagaaataa
     6541 atattcaata cgatagtagc actttattta atacaattca atttttacct aaagataccc
     6601 aagctgtaaa aggagttgtt ttatactttc atggcaatag agaaaatata aatagatatg
     6661 ccccttatgt tgctaatttt acaaaaaatg gctacgaagt ttggataact gattacccta
     6721 catttggcaa aagcactggt accctttctg aaaaagtttt agaaactcaa gcattagaaa
     6781 tatacaaaat agcagaaaca aagtttaaag attcttctat tattttatat ggcaaaagcc
     6841 ttggtacagg ccttgctgct tatgttgcat caagaaaaaa ttgtaaacaa ctaattttag
     6901 aaactcctta tagcagtata acttctttaa tgaagagata ttgttttatt tatcctatag
     6961 aaaagatgat tcgttataag ttgccagtaa ttgaatatat aaaaaaagca acagcaccca
     7021 taattatttt tcaagcaagc gaagattggg tgatacccaa tagcaatact gaaatgctaa
     7081 aagtaaactt aaaggcaacc gataagtttg taaatattga aggtgcttct cacaatacca
     7141 ttaataatta tcctcaatat cataaaacct tagatagttt attacaataa ctacaattgc
     7201 aaatcattca tataagcaat atcattggtt ttattaaatt tcttttgcag aaaagaattt
     7261 ggcaaaagtt taaacacaga aaataaaaca ttatactgcc atttttttcg ttgatgacta
     7321 aacctgctaa atgcaaatga agcgttatta ataaatttag ttttaggctt tcttttaaac
     7381 tgataaagct caaaagcatc tttaaaatta ttgggatatt tttttaaaca agcagccaac
     7441 gtgtatgcac tttctattgc ctggcatgcc ccttgtgcaa tatctggtgt acaagcatga
     7501 atacaatctc caacaaaaac tattctatta ttataccaat tatttaaatt attgaattta
     7561 atatgcctta atggtgtttt aataatatta ttgtctgcat caattactgc atgggcatat
     7621 tcaggaaagt cattaaactc ctggtgtaaa aattgtttta gattttcttc ttcgtaaaaa
     7681 attccatctt ttatccaacg tatgcaaaac caaaaataat catccccccc tttatgaata
     7741 atattaaatc gcttgttttt accccacatt tcatgagctg catttttaaa cttatcgggt
     7801 aggtttaatg ttgcaatacc acgccacatt atttgccctg tataacttgg ttgaatgtga
     7861 ggcgaaaatt tatttcttgt aacagagtgc acaccatcgg cagctactac aatgtcaaaa
     7921 aaataggttg tattatttgc atcaattgta acgccactct cgttttgttc tattttttca
     7981 attcttgcat ttaatgtata atcctcttgc tgtaaaggtt tgcttaatac ttctatcaac
     8041 cttgctctat gtattccata accaatttca ttagaaggta ttcttttaat tgtttgaagg
     8101 tgatggttag taatattaaa atctttaatt aaagtagatg ctttttcaaa atcatttcct
     8161 acacctaatt gatgtaataa atatttagta ctactactta gcataacacc tgcaccaata
     8221 tctttaaaag cagaagcctg ttcaaataaa tgaaatgaaa tatttgcttt tttaagagcc
     8281 aatgcagtac ttaatcctgc aactcctcca cctattatgc caactttcat gcagtaaatt
     8341 tggtttctat ttttataagt acaaaataaa tttagccata aaataccatt tattgggtat
     8401 atgaatataa gaagagttga atgaaatttg attacattaa tgtggatact ttatacctta
     8461 taatgtaaat aaaatattat aaaacttaca atgaaaagtt tacttctgct aacactaatt
     8521 tgctttattt ataatggcaa ggcacaaggc ttgtacccta cttatgttaa tgaaaatgtt
     8581 gtatatattg attctacggg aagtaaaaag ttagaaacaa aacacaaacg ttgtggttta
     8641 taccaaaatg gtttagcatg ggtaattatt aacgaagcta ttggctttat gaatacaaaa
     8701 ggagaagtga taatagagcc aaaatattct gaaacatctg gtttttatga aggatttgca
     8761 agcgttgtag ttgataatgc ctggggagta attaataaca aaggagaaac aatagtagag
     8821 cctaaatatt atgaaattgg aatttatagc gatggaatgt gtaagtttaa agagaataaa
     8881 agcttatttt ctgaatgggg ctttttagat acaaaaggca aagtggctat taggccaact
     8941 tttgaaaata ttgatgattt tcatgaaggg ctagccatgt ttattacaga tgcaaaagca
     9001 ggctatatag atacaactgg taaagtagta attgaacctt ctttttatta tggtgcattt
     9061 tttaaaataa atggtaaagt agtttttgaa aaaggtgcat ttagtaatgg cttagctgcg
     9121 gtagaaataa ataataaatt tggctttata gataaaacag gaaaagtaat tattgcagca
     9181 caatacgatg atgtaaaaaa tttcagtgaa ggtttggctg cagttaaaat aaaagaaaac
     9241 tggggcttta tagatactaa aggaagtata attattacac ctacttatgc aaacgttgaa
     9301 agctttagcg aaggtttggc tgcagttaaa ataaaagaaa actggggctt tattaatacc
     9361 aaaggcaaat taattatacc tgcaacttta tttggctcta ttgatattgg aatagattta
     9421 aagtttaaag atggagtttg ccaagctatg ttaactgata aagtatgggg ttatataaat
     9481 aaacaaggaa aaacactttg gaagagtaag taaagagtat taaaaacgga agaatttata
     9541 ttcttccgtt tttaatttca tctacaatat tggggtctaa caaagtagta gtatcgccta
     9601 aattttctac ttctccttca gcaatttttc ttaatattct acgcattatt ttaccacttc
     9661 ttgttttagg caaaccggct acaaactgaa ttttatctgg ttttgcaata gcaccaatta
     9721 ctcttgttac agtttgtaaa atatcttttc tggtaagttc atcatcacta tgttttgagc
     9781 ttactataac aaaagcataa attccttgtc ctttaattgc atgagggtaa cccactactg
     9841 cactttcaac aacacctaca tgcatattaa tggcattttc aacttctgca gtaccaattc
     9901 tatgtccact aatatttaaa acatcatcta ccctgccagt aattctgtag ttaccatctt
     9961 tatctttcaa tgcaccatcg ccagaaaaat acagattttg ataggttgca aaata
//
LOCUS       DAOQHP010000034        10002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3423_length_10002_cov_3.836634, whole genome shotgun sequence.
ACCESSION   DAOQHP010000034 DAOQHP010000000
VERSION     DAOQHP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_10002_cov_3.836634"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PLY81_02130"
     CDS             <1..1350
                     /locus_tag="PLY81_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HNF29058.1"
                     /translation="IWYEKANQVHTLSNNVFKYVEDLKLELMKEAGFDPNNKKDGKTD
                     SSFKEDNLEAATRLLVETAPQGKGKGKELFKKLQDFKSALFGLDSAVVEELGKSLPID
                     LSMPKTKTLGNNTWEAAYFRMTPTIAALTILSKFQNDIRNTEAQVVELFHKKVGEVKI
                     KYDQFKAIANASASYVMPSEEIIINAGVGAFSSAAKPSVTIDGAGAQALPDGSYEYKF
                     NAPTAAGEYTKKVRISFVKPDGTTATVDKDIKYTVGQLAGLTVSTDATRVFYSGGIEN
                     PLSVTGQGGSEKIQLSVSGTGVTVESRGKGQYIVKCTQPGAAILKASDGKSTQEFKIP
                     IKRVPDPVIMVNGNRGGSMEAGKFKAAGGVSAKLLDFIFEGYTFKVSSFALFFTGTGF
                     EEKNEVVEVQGNAFNAEARRFMNRCAAGSTVVIGAVRVADQIGNVREVEGTISFTLE"
     gene            1378..2472
                     /gene="gldN"
                     /locus_tag="PLY81_02135"
     CDS             1378..2472
                     /gene="gldN"
                     /locus_tag="PLY81_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNF29059.1"
                     /translation="MKKLFLGIVVISSVIGFKNDAFAQFKKAKKTEEKTDAGKTGTTG
                     TTGTGGVTSTTSTSTSTAKKDTTIIKPGKRFDTTRDVSGKEYGGVVPTSKRNAYGYTK
                     EPVSDRKPLPYDHIREDDATFSHFVWREIDAREKMNLPFIYSAKDETGDQRFFSIILN
                     AIKNDSVVAFDAMDDRFTTPLSINQVISMSSAQSTLLDTTYAENPNDENVMDTVIKWK
                     ENLKAPKPDSIYKFRIKEQWMFDREASKFLVRIIGIAPLAPNPLPPGVKKSNAPPTYY
                     PLFWVYYPDLRPTLSRTFAYNPKNIGGQLSWEEVFEGRFFSSYIIKSTVNNPGDKTLA
                     QLIEDPLFRLLEGENIKEKLFNYEQDLWSY"
     gene            complement(2522..2983)
                     /gene="bcp"
                     /locus_tag="PLY81_02140"
     CDS             complement(2522..2983)
                     /gene="bcp"
                     /locus_tag="PLY81_02140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNF29060.1"
                     /translation="MTILTIGKKAPAFTGKDQNSNKVSLADFKGKKVVLYFYPKDSTP
                     TCTVQACNLRDNFALLKQQGIEIIGVSADDIKSHQKFANKNKLPFSLIADTAKIIIEK
                     YGVWAEKKLFGIKYMGIRRTTFVIDEKGIILAIITKPKSKSHAEEILKVIA"
     gene            3301..4947
                     /gene="carB"
                     /locus_tag="PLY81_02145"
     CDS             3301..4947
                     /gene="carB"
                     /locus_tag="PLY81_02145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNF29061.1"
                     /translation="MPKDNSIKSVLIVGSGPIIIGQACEFDYSGTQAARSLREEGIKV
                     ILINSNPATIMTDPMMADKVYLLPLTVESIEQILEENQIDAVLPTMGGQTALNLCKEV
                     DELGIWNKFNCKLIGVDVEAINTAEDRDLFRQLMLKIGISVAPSHVANSFLEGKEHAQ
                     KIGFPLVIRSSFTLGGSGGGFVHNKEELDDALKNGLEASPIHEILVEKAVLGWKEYEL
                     ELLRDKNDNVVIICTVENFDPMGVHTGDSITVAPAMTLSDTAFQNMRNQAILMMRSLG
                     NFAGGCNVQFALNPETEELIAVEINPRVSRSSALASKATGYPIAKIAAKLAIGYTLDE
                     LKNQITQTTSAYFEPALDYVIVKIPRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTE
                     AIQKACQSLENNAVGLGYYGKSMMKSDEIVEYIKTPKWDRIFRIKDALMQGVTVKTIA
                     QATGIDKWFLFQIQKICTIEKELDKYTLEDIPTNLLKEAKTNGFSDEQIAKILSGNVT
                     EEEVYEKRKALGITRVYKMVDTCAAEFEAKTPYFYSTFEG"
     gene            complement(4953..5732)
                     /locus_tag="PLY81_02150"
     CDS             complement(4953..5732)
                     /locus_tag="PLY81_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29062.1"
                     /translation="MKLVVLFSFIFLYACNANNNNVHHKNNSPVEYLKNQITSYPDSV
                     ELRMQLIDVLDSMKLYKEALFELDVLIKKDSFNAENWYYKAQLYEKSKDTVAAIINYK
                     RAIRIYPSPDNILTLANLYAEYRNDTALILCVEIETTYTDKKYSADCMFIAGVYYART
                     GNFTKAMELFDLCINNSYSYIVAYLEKGFIYYDNKDYKKALEIFKLSAQVNATYADSY
                     YWQAKCYEALNDKQNAISNYEKALILDKNLQEAEVAIKRLK"
     gene            5823..6908
                     /locus_tag="PLY81_02155"
     CDS             5823..6908
                     /locus_tag="PLY81_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29063.1"
                     /translation="MKKIILLLAVVSSYASYAQHSHQSQPVVDSMHYANEKHFKNIQQ
                     LTFGGDNAEAYFSFDGKYLIFQKTNPKEGIDCDQIFIGKLPKKGEPFNYKMVSTGRGR
                     TTCAAFLKDGKHFIYASTHLGGNECPPVPDRKKYGNRYIWPLYSSFDIFMADTSGKIV
                     KQLTNQPGYDAEGTISPDGKTMVFTSTRDGDLELYLMDLKTLKVKRITNTLGYDGGAW
                     FSPDGKKIIWRASRPKTDAEVKEYKELLADNLVAPNNMEIWVANADGSNAKQITNFGQ
                     ANWAPYFMPDSKRIIFASNHEYKRGFPFNIYTINGDGSKLTKVSRDKGFDAFPMFSYD
                     GKKIVFCSNRNNGGTRDTNIFIADWEE"
     gene            6965..7393
                     /locus_tag="PLY81_02160"
     CDS             6965..7393
                     /locus_tag="PLY81_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29064.1"
                     /translation="MYNGLLHLHNFLRWVIIILLVINIIKAFSGNKNIKLSLWLLISS
                     HITLLIGLYQYFFGAMGFKLFGTYGAEVMKNSALRFWAVEHIMGMVIAIALITIGHIS
                     LKKQATKKASILFLVALIIILAVVPWPFREGIGRAWFPGM"
     gene            7571..7717
                     /locus_tag="PLY81_02165"
     CDS             7571..7717
                     /locus_tag="PLY81_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29065.1"
                     /translation="MLLISYQFLINDQYILDSVKTNNKLIQADSIIVEFNTNSPLQHK
                     AIIP"
     gene            7971..8771
                     /locus_tag="PLY81_02170"
     CDS             7971..8771
                     /locus_tag="PLY81_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF29066.1"
                     /translation="MARIIGVANQKGGVGKTTTAINLAASFAVLEFKTLLVDADPQAN
                     STTGVGFDLHNITTSLYDCMVNNAKANEVILKSDIPNLDLLPSHIDLVGAEIEMINYP
                     NRENVMRLLLDEVKSKYDFIVIDCSPSLGLITVNSLVAADSVVVPVQCEFFALEGLGK
                     LLNTIKIVQSRLNPELQIEGILMTMYDGRLRLSNQVVSEVRRHFDEMVFSTIIHRNTR
                     LSEAPSVGKPVILYDADSKGAVNYLNLAKEVLQKNEMTKMTNEERIIE"
     gene            8821..9738
                     /locus_tag="PLY81_02175"
     CDS             8821..9738
                     /locus_tag="PLY81_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF29067.1"
                     /translation="MTTPKQNKDAIGKGLRSLLNNIDQDLKNTSGSLKSTVIEQTTGI
                     VRVPVADILVNPKQPRRDFDETELSELATSIKIHDIIQPLTVSKLANGKYQLIAGERR
                     WRASKIAALKDVPVYIRTANDKELLELALLENLQRTDLNAIEIGLSYKRMMEELNYTQ
                     EQVADRMGKERSTVTNYIRLLKLPPDIQLAVRNGKLSMGHARALITIDTVDKQLFVFS
                     EIKNKQLSVRQTEDLVRKLYKGIDTAKTPAKNNLPPAYKKIEDNLASHFSTKVNLLHN
                     KKGHGSITIEYYSLQELNKILDQMNIVVS"
     gene            9747..>10002
                     /locus_tag="PLY81_02180"
     CDS             9747..>10002
                     /locus_tag="PLY81_02180"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNF29068.1"
                     /translation="MKIYKGILLLVFCSCILIITTSNSNAANFQRLDTSKPIVENMPS
                     TTKSKAKHNPKVATRRSAILPGWGQAYNKEYWKIPIVYGAL"
BASE COUNT         3661 a         1362 c         1767 g         3212 t
ORIGIN      
        1 atttggtacg aaaaagccaa tcaagtacat actttaagca ataacgtatt taagtatgta
       61 gaagatttaa aattagaatt aatgaaagaa gctggttttg atccaaacaa caaaaaagat
      121 ggcaaaacag actcttcatt taaagaagat aacttagaag cagcaacaag attattagtt
      181 gaaactgctc cacaaggaaa aggaaaagga aaagagttat tcaagaaatt acaggatttc
      241 aaatctgcat tatttggttt agatagtgct gtagtagaag agttaggcaa aagcttacca
      301 attgatttat ctatgcctaa aacaaaaact ttaggtaata acacttggga ggctgcttat
      361 tttagaatga ctccaacaat tgctgctcta acaatattaa gtaaattcca aaacgatatt
      421 agaaatacag aagcacaagt ggttgaatta ttccacaaaa aagtgggcga agtaaaaatt
      481 aaatacgacc aatttaaagc tattgccaat gcttctgcat cttatgtaat gcctagcgaa
      541 gaaataataa ttaatgctgg tgttggtgca tttagtagtg ctgctaaacc tagtgtaact
      601 attgatggtg ctggtgctca agctttgcct gatggtagct acgagtataa atttaatgcc
      661 cctactgctg caggtgaata tactaagaaa gtaagaataa gttttgtaaa gcctgatgga
      721 acaacagcta ctgttgacaa agatattaaa tatactgttg gtcagttagc tggtttaaca
      781 gtatccacag atgctacaag agtattttac tctggtggta ttgaaaaccc acttagtgtt
      841 actggtcaag gtggctctga aaaaatacag ttgagtgtat caggtacagg tgtaactgtt
      901 gaatcaagag gaaaaggaca atatatagtt aaatgtacac aacctggtgc tgctatttta
      961 aaagcaagtg atggtaaatc tacacaagag tttaaaattc caattaaacg tgtaccagac
     1021 cctgtaatta tggtaaatgg taatcgtggt ggtagtatgg aagctggtaa gtttaaagct
     1081 gctggtggtg tatctgccaa attattagat tttatatttg aaggatatac ctttaaagta
     1141 tcttcttttg cattattctt taccggaact ggttttgaag aaaaaaatga ggttgtagaa
     1201 gtacaaggta atgcatttaa tgcagaagca agaagattta tgaatagatg tgcagcaggt
     1261 tctacagttg ttattggtgc tgttcgtgtg gctgatcaaa ttggtaatgt aagagaggtt
     1321 gaaggtacta tctcctttac attagagtaa ttgtaaatta taaattaatt ctttattatg
     1381 aaaaaactgt ttttgggtat agttgttatt tcttctgtaa taggttttaa aaacgatgca
     1441 tttgcacagt ttaaaaaagc caaaaaaaca gaagagaaaa cggatgctgg taagacaggt
     1501 acaacaggta caactggtac tggtggagtt acttcaacta catccactag cacctcaact
     1561 gctaaaaaag acactactat aattaaacct ggaaaacgtt ttgatacaac tagagatgtt
     1621 tctggtaaag aatatggtgg tgtagttcct acaagcaaaa gaaatgctta tggatatacc
     1681 aaagaaccgg taagcgatag aaaaccttta ccatacgatc atattagaga agatgatgct
     1741 acatttagtc attttgtttg gagagaaata gatgcaagag agaaaatgaa cttgccattt
     1801 atttactctg ctaaagacga aactggagac caaagattct tttccataat attgaatgct
     1861 attaaaaacg acagtgttgt tgcttttgat gcaatggatg ataggtttac tactccttta
     1921 tccatcaatc aggttatcag tatgtcatct gctcaatcta ctttgctaga tactacttat
     1981 gcagaaaacc ccaatgatga aaatgtaatg gatactgtaa ttaagtggaa agaaaattta
     2041 aaagctccta aaccagactc tatttataaa tttagaatta aagaacagtg gatgtttgac
     2101 agagaagcta gtaaattttt agtaagaata attggtattg cacctttagc accaaaccct
     2161 ttacctcctg gtgttaaaaa aagcaatgct ccaccaactt actatccttt attttgggtt
     2221 tattatcctg atttaagacc aacactatca agaacatttg cttataatcc caaaaatatt
     2281 ggtggccaac tttcttggga agaggtattt gaaggaagat tttttagtag ctatataata
     2341 aaatctaccg ttaataaccc tggcgacaaa acattggctc aattaattga agatccattg
     2401 tttagattat tagaagggga aaatattaaa gaaaaattat ttaattacga acaagactta
     2461 tggtcgtact aattgagata taattttagt aaatcccact taaataatta agtgggattt
     2521 tttatgctat aactttcaaa atttcttctg catgtgattt gctttttggt tttgtaataa
     2581 ttgccaggat aatacccttt tcatcaatta caaatgtggt tcttctaatt cccatatatt
     2641 ttataccaaa taattttttt tcagcccaaa cgccatattt ttcaataata atttttgctg
     2701 tatctgctat taatgaaaat ggtaatttat ttttatttgc aaatttttga tgacttttaa
     2761 tatcatctgc actaacacca ataatttcta taccttgctg ctttaataat gcaaaattat
     2821 ctcttaggtt acaagcttgc actgtacagg taggtgtact atccttagga taaaaataaa
     2881 gtactacttt tttcccttta aaatctgcca gagaaacttt attgctattc tgatctttac
     2941 ctgtaaaagc aggagctttt ttgcctattg taagtattgt catttaaata aaaattaatt
     3001 gattgttgaa agaataaaaa taaatcattt agatggttgt tagaataaaa atagtttaag
     3061 taaaaaagca attaaaaagc ctattgccgt acatttgcac aatttgtttt ttctgtttcc
     3121 actaatgtac agaggatatt atttagacta ttggagaaac ctgttgagtt tttgtaaaat
     3181 agttgagtag atgccaccta ttgttggcat gacaagtaca gaaaagaaca aggtgtacaa
     3241 gagtgcgacg caacaaaagc caaatagcaa tataaaagct aagtaaataa aaataaaatt
     3301 atgcctaaag ataattccat aaaatctgtt ttaattgttg gcagtggtcc aataattatt
     3361 ggtcaggctt gcgagtttga ttatagtgga actcaagctg caagaagttt aagagaagag
     3421 gggattaaag ttattttaat taatagtaac cctgctacca taatgactga ccctatgatg
     3481 gcagataaag tttatttgct accattaaca gtagaaagta ttgaacaaat tttagaagaa
     3541 aaccaaatag atgcagtgtt accaacaatg ggtgggcaaa ctgccttaaa cctttgtaaa
     3601 gaagtggatg aattaggaat ttggaataaa tttaattgta agcttattgg tgtagatgta
     3661 gaagcaatta atactgcaga agatagagat ttgtttagac aattaatgct taaaatagga
     3721 attagtgtag caccaagcca tgtggcaaat agttttttag aaggaaaaga gcatgcacaa
     3781 aaaattggct ttccgttagt aatacgttca tcatttactt taggaggtag tggaggagga
     3841 tttgtgcata ataaagaaga actagatgat gctttgaaaa atggattaga agcaagccct
     3901 attcatgaaa ttttagtaga gaaggctgtt ttaggctgga aagaatatga attagaatta
     3961 ctaagagata aaaatgataa tgttgttatt atttgtacag tagaaaattt tgacccaatg
     4021 ggtgttcata caggcgatag tataactgtt gcacctgcca tgacattaag tgatacagca
     4081 tttcaaaata tgcgaaatca agcaatatta atgatgcgaa gcttgggtaa ttttgctggg
     4141 ggttgtaatg tgcaatttgc attaaatcca gaaactgaag agttaatagc agtagaaatt
     4201 aatccacgtg taagccgtag tagtgctttg gcaagtaaag caaccggtta tcctattgca
     4261 aaaattgcag ctaaattagc cataggatat actttagacg aattaaaaaa tcaaattacg
     4321 caaacaacaa gtgcctattt tgaaccagct ttagattatg taattgtaaa aattccgcgt
     4381 tggaattttg ataaatttaa aggagccaac gatactttag gcttacaaat gaaaagtgtt
     4441 ggcgaggtaa tggcaattgg cagaagtttt acagaagcca ttcaaaaggc ctgtcagagt
     4501 ttagagaata atgcagttgg tttgggttat tatggcaaga gtatgatgaa aagtgatgag
     4561 attgtggagt atataaaaac tcccaaatgg gatagaatat ttagaattaa agatgcttta
     4621 atgcaaggtg taactgttaa aacaattgct caggcaacag gtattgataa gtggttttta
     4681 tttcaaattc aaaaaatttg taccatagag aaggaactgg ataaatatac tttagaggat
     4741 ataccaacaa atttattaaa agaggctaaa accaatggat ttagtgatga gcaaatagcc
     4801 aaaattttaa gtggtaatgt taccgaagaa gaagtatatg aaaaaagaaa agcattaggc
     4861 attacaagag tgtataaaat ggtagatact tgtgcagcag agtttgaagc taaaacgcct
     4921 tatttttata gcacttttga aggttaataa ttttatttta accttttaat agctacttca
     4981 gcttcctgta aatttttgtc taatattaag gctttttcgt aattacttat tgcattttgt
     5041 ttatcgttta atgcttcgta acatttagct tgccagtaat atgagtcggc ataagttgca
     5101 ttaacttgag cagaaagttt aaaaatttct aatgcctttt tataatcttt attatcgtaa
     5161 taaataaagc ctttttctaa ataagcaaca atataagaat agctattatt aatacaaagg
     5221 tcaaaaagtt ccattgcttt agtaaaattt cctgttcttg cataatacac acctgcaata
     5281 aacatacaat cggcagaata ttttttatcg gtataagttg tttctatttc aacacataaa
     5341 attagtgcag tatcgtttct gtactctgca taaagatttg caagggttaa aatattatcg
     5401 ggagatggat aaattctaat tgctctttta taattaataa ttgcggcaac agtatcttta
     5461 gatttttcat aaagttgtgc tttataatac caattctctg cattaaaact atcttttttt
     5521 attaaaacat ctaattcaaa taacgcttct ttatatagtt tcatactatc taaaacatca
     5581 attaactgca ttctcaattc aacactatct gggtaacttg taatttggtt ttttaaatat
     5641 tctacaggtg aattgttttt gtgatgtaca ttattattgt ttgcattaca tgcataaaga
     5701 aaaatgaaag aaaataatac aactaatttc attgcacaaa aataaaaatc cttagtaata
     5761 attttacatt tatctgacat ttatccaaag ccttttaata gttttcacat ttactttgca
     5821 gcatgaaaaa aattatactt ttattagctg ttgtaagtag ttatgcaagc tatgcacaac
     5881 atagccatca atctcaacct gttgttgact ctatgcacta tgcaaatgaa aaacatttca
     5941 aaaatattca gcaactaact tttggtggcg ataatgccga agcttatttt agttttgatg
     6001 gaaaatattt aatttttcaa aaaacaaacc ctaaagaagg cattgactgt gatcaaattt
     6061 ttataggaaa attaccaaaa aaaggcgaac catttaacta taaaatggta agtactggca
     6121 ggggtagaac aacttgtgct gcatttttaa aagatggtaa gcattttatt tatgcctcta
     6181 cacatttagg tggaaatgaa tgcccacctg taccagatag aaaaaaatat ggcaatagat
     6241 atatatggcc tttatacagc agctttgata tatttatggc tgatacaagt ggcaaaatag
     6301 ttaaacaatt aacaaaccaa cctggatacg atgcagaagg aactatttcg ccagatggta
     6361 aaacaatggt ttttacaagc acaagagatg gagatttaga attgtactta atggatttaa
     6421 aaacgcttaa agtaaaacga ataaccaata cattagggta tgatggtgga gcatggttta
     6481 gcccagatgg caaaaaaata atttggcgtg ctagtcgccc taaaacagat gcagaagtga
     6541 aagaatataa agagctatta gccgataatt tggtagcacc taataatatg gaaatttggg
     6601 ttgccaatgc cgatggcagt aacgctaaac aaataaccaa ttttggccaa gctaactggg
     6661 caccttattt tatgccagat agcaaaagaa ttatttttgc tagtaaccat gaatataaaa
     6721 gaggctttcc gtttaatatt tataccatta atggagatgg tagcaagtta acaaaagtta
     6781 gtagagataa gggttttgat gcatttccta tgtttagtta tgatggtaaa aaaatagttt
     6841 tttgtagtaa cagaaacaat ggtggtacaa gagatactaa tatttttata gcagattggg
     6901 aagaataaac taatatatct atatacaaaa caaataccct tagttttgta aaaatctacc
     6961 aattatgtac aacggattat tacacttaca caatttttta cgttgggtaa taataatatt
     7021 attagtaatt aatattatta aagcctttag cggaaataaa aatattaaac ttagcttatg
     7081 gctgttaata agtagccata ttacactatt aatcggcctg tatcaatact tttttggggc
     7141 aatgggtttt aaactttttg gaacttatgg tgccgaagta atgaaaaata gtgccttacg
     7201 cttttgggct gtagagcata ttatgggtat ggtaattgcc attgctttaa ttactattgg
     7261 gcatatatcg cttaaaaaac aagccactaa aaaagcatca attctatttt tggttgcctt
     7321 aataattatt ctagcagttg taccttggcc ttttagagag ggtattggaa gagcttggtt
     7381 tcctggtatg taattaactt ttaatttttt aaaaaggttt cgtttacgaa gccttttttg
     7441 ttttatttgc agtatgaatt ttaaaatact agctttttgt agtttcataa taattttact
     7501 aattgcttgc aacagtaata aacaacaatg taaagcaaag attatagaaa gaaaactaac
     7561 taccgactct atgttgttaa ttagctatca atttctaata aatgaccaat atattttaga
     7621 tagtgttaaa acaaacaata aactaataca agcagatagt attattgtag agtttaatac
     7681 taatagccct ctacaacaca aggcaattat tccttaacca ctatttttat aataatttgt
     7741 aaaaagtact ttgtataaaa ttgaatacct tgttgtagag ataaaagcat gaatatattt
     7801 agttccttct gtgtataaat taatcaaatt atatttaaac aatttaaggg gaaaaaagtg
     7861 gggaaaatta ctacactaaa gcattgataa atttcggttc tttgtagagt aacttattat
     7921 tataaatttg taatttttga aagtttttaa aatagaagat aacgagaatt atggctagaa
     7981 ttattggtgt agctaatcaa aaaggtggcg ttggtaaaac aaccactgca attaatttag
     8041 cagcaagttt tgctgtgcta gaatttaaaa ctttgttggt tgatgctgat ccacaagcta
     8101 atagtacaac aggggttggt ttcgaccttc ataatattac cactagtttg tacgattgta
     8161 tggtaaataa tgccaaagca aatgaagtta ttttaaaaag tgatattcct aatttagact
     8221 tattaccctc tcatatagat ttagttggtg cagaaattga aatgattaat taccccaaca
     8281 gagaaaatgt aatgcgtttg ctattagatg aggtaaaaag caaatacgat tttattgtaa
     8341 ttgactgctc cccatcatta ggtttaatta cagtaaattc tttagtagca gcagatagtg
     8401 tagtagtacc tgtacaatgc gaattttttg ctttagaagg tttaggaaaa ctattgaata
     8461 ctataaaaat tgtacaaagc agattaaatc ctgaattaca aattgaagga atattaatga
     8521 ccatgtatga tggacgttta cgtttaagta accaagtggt tagtgaggta agaaggcatt
     8581 ttgatgaaat ggtgtttagc actattatac acagaaatac tagattaagt gaagccccaa
     8641 gtgttggtaa accagttatt ttgtatgatg cagatagtaa aggtgctgta aactatttaa
     8701 accttgctaa agaagtttta caaaaaaatg aaatgaccaa aatgaccaac gaagaaagaa
     8761 taattgaata agcatatctt tcgaacgctt caacaagtta aataatttta tttgcattaa
     8821 atgacaacgc ccaaacaaaa taaagatgct attggcaaag gcttaagaag ccttttaaat
     8881 aatatagatc aagatttaaa aaatacttca ggctcattaa aaagtacagt tattgagcaa
     8941 actacaggca ttgtacgtgt accagtagcc gatattttag ttaatccaaa acaaccaaga
     9001 agagattttg atgaaacaga attaagtgaa ttagctactt ctataaaaat tcatgatatt
     9061 atacaaccat taactgtaag taaattagcc aatggaaagt atcagttaat agcaggcgaa
     9121 agaagatgga gagcttctaa aattgcagca ttaaaagatg ttcctgttta cattcgtact
     9181 gcaaacgata aagaattatt agagcttgct ttgttagaaa acctgcagcg tacagattta
     9241 aatgcaattg aaattggact aagctacaaa cgtatgatgg aagagttaaa ttatacgcaa
     9301 gagcaagtag ctgacagaat gggtaaagaa agaagcactg taaccaacta tattcgttta
     9361 ttaaaacttc cgccagatat acaattggct gtaagaaatg gcaaattatc tatggggcat
     9421 gcaagggcct taattaccat tgatacagtt gataaacaac tttttgtttt cagcgaaata
     9481 aaaaacaaac aattaagtgt aagacaaaca gaagacttag ttagaaaatt atataaagga
     9541 atagatactg ctaaaactcc tgctaaaaat aatttaccac cagcctataa aaaaattgaa
     9601 gataatttag cttcacattt tagcactaaa gtaaatttat tgcacaacaa aaaagggcat
     9661 ggcagtatta ccatagagta ttactcatta caggaattga ataaaatttt agaccagatg
     9721 aatattgttg taagctaaat tttttaatga aaatttataa aggcatattg ttgttggtgt
     9781 tttgtagttg tatcttaatt attacaactt caaattcaaa tgcagccaac tttcaaaggt
     9841 tagatactag taaaccaatt gtagaaaata tgccttctac aaccaaatca aaagcaaagc
     9901 ataatccaaa agttgcaaca agacgttcag ctattttacc aggttggggg caggcttata
     9961 ataaagaata ctggaaaata cccattgtgt acggtgccct tt
//
LOCUS       DAOQHP010000035         9856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3488_length_9856_cov_3.876033, whole genome shotgun sequence.
ACCESSION   DAOQHP010000035 DAOQHP010000000
VERSION     DAOQHP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3488_length_9856_cov_3.876033"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLY81_02185"
     CDS             <1..855
                     /locus_tag="PLY81_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF29069.1"
                     /translation="KADENEKTPIGVLLTIFAVVIMFWAVFKQNGTALNTWADRYTDR
                     SVSGTTEKVFKAVQFTQTLKYQKDSVPLYDSMFRLQKQNGVVIKEFNYPVYFRNDMRS
                     KMQTEGGELSIWATNLSQSINPAWVIILTPLIVAFFTFLRGRKKEPSTPTKIAFGLFV
                     SALSVLVMIAAVKAGNNGAEKVSVWWLIGSYGVVTIGELLLSPMGLSLVSKLSPVRIT
                     SLMMGGWFLSTSIGNKLSGVLASMWDKYDDKANFFWLNFFLLLFSASIMFLMLKRLNK
                     VLKVRGVK"
     gene            complement(926..1807)
                     /locus_tag="PLY81_02190"
     CDS             complement(926..1807)
                     /locus_tag="PLY81_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNF29070.1"
                     /translation="MFYFYYALFYLLSLLPWRVMYFLSDCFYLLAYYVIRYRRNVVAN
                     NLQIAFPDKTERERIKISKAFYHNLIDSFVESIKLLTISRKEFNKRCITNFKVVNDLY
                     STGQSVQLLSGHFFNSEFLNLGCAANLKYPFLGVYMGLSNKNLEKVMVIMRKKFGTIL
                     FPASEFRTEFPKHSKSVYSIGLIADQNPPIPTNAVWFPFFGRMTPFHTGPEKGAIRMN
                     TAIAFIEMTKVKRGYYKLEAKLVTTTPKEFLEGTITKQLVAFIEETVKQHPDNYLWSH
                     RRFKYEFDEKKYGHLVI"
     gene            1930..2910
                     /locus_tag="PLY81_02195"
     CDS             1930..2910
                     /locus_tag="PLY81_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNF29071.1"
                     /translation="MKFLVIRFSSIGDIVLTTPVVRCLKQQVTNSEIHFLTKASFSTI
                     VENNSYIDKVVLLNNNWDETILQLKQENYDYIIDLHHNLRTLRIKKALKIKSFSYNKL
                     NIQKWLLTAFKINTLPPIHIVDRYIETVKKIGVINDGKGLDYFIPEKDCIKQNDIPAQ
                     HQFGYIGIVIGAALNTKKMPVNKLIDLCSKIDFPIILLGGKEDKENGDTIAAIDTIKI
                     YNACGKFNLNESADLVRKAKLIITHDTGLMHIAAAFKKPILSVWGNTIPSFGMSPYFG
                     NNQITNLHYEVKLGCRPCSKIGFNKCPKKHFKCMEQQDVSQIAKDALKLV"
     gene            complement(2931..4145)
                     /locus_tag="PLY81_02200"
     CDS             complement(2931..4145)
                     /locus_tag="PLY81_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNF29072.1"
                     /translation="MAASKPVISTSFSYETLEETLDIKPDGAKLHIGIPKEIAFQENR
                     VALSPDAVGVLVSNGHTVMVETKAGEGCNYADKEYGEAGATITYDRTEVFKAPVLIKT
                     APPVQEDLPLIQNGQTIISPIHYSALNKATVKKLMEKKVTAISFDNLKDDSGTFPIVR
                     SMSEIAGGAVMLIAGQYLSSFNKGKGVLLGSISGIPPTKVVIIGAGIVGEVATRNALA
                     LGASVKVFHNNVYKLKQLQNNLGQRIWTSVLEPKILAKQLKTCEVAVGALSNAYGHSP
                     VVVTEEMVANMRKGSIIIDVAIDRGGCFETSQLTSHENPTFLKYDVIHYCVPNIPSGF
                     ARTASQAISNVIMHLILQISNEGGLDEIIWHNFNIREGIYLFKGALTDFYISQKFDLK
                     YTDLNLLIASRR"
     gene            complement(4236..4844)
                     /locus_tag="PLY81_02205"
     CDS             complement(4236..4844)
                     /locus_tag="PLY81_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29073.1"
                     /translation="MKNKFSFLNFSACFLLIITTLLACNSVYTPKPKGYFKINFPEKK
                     YTTFQMKDYPYSFEYPVYAKVVKDSLFFGAKTENPWWINIDFPQFNGRIYVSYKLISN
                     NNFNDLVEDAFKLTNKHTQKATSIDDSLIITPNNVHGIYFKVGGDVATANQFFLTDSV
                     THFLRGALYFDATPNEDSLKIVNNFLLEDVKHLINTLRFTKK"
     gene            complement(4890..6911)
                     /locus_tag="PLY81_02210"
     CDS             complement(4890..6911)
                     /locus_tag="PLY81_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF29074.1"
                     /translation="MAIAALLLVNSCNYSHKPEPINFKANEELENKFITFHLTDSVFL
                     NYTLDSLKKIYPQSHNTKVLYYYLLYLKEHYTNNYYENYTNAEALLNYLENNKLTTVY
                     SKYYTDALFAKGEYFFAARKYEEALKEYYKAKLILDAQTDTCNYSKYYNKLWNFYYSK
                     KDYRKALDYLNISIDREEKCKNNTAYFLYFHRIQSTLNTKALCYEKLGAYDSAIIFYQ
                     QGIKFIQNQESIHEINNQKRELALGIYYGNLGGAFAYNKQPDSAIYYLNKSIHINSKP
                     GYDSIDLQTAYLKLVDVYLSKKQYAKAKDILDFSDRLINSDSHPTIGYIRSKQRLLYY
                     NYYYQSGQLEKAIKAFESFFHVQDSVNQIAYDLNNFSIGKEVDKIKSEYLLKNFEKKN
                     EIKTIYIIVFSIVMALLFVIIILILRYLKQIKITANQTTLHNLQLKSTLQQFEQSNKN
                     FARVMKVMAHDLRSPLAGITGITDILLNDSTIDENEKKEMLTLIQSSSNNSLSMITEL
                     LNTSSNLEQKELVKEEINIIELLKQCTELLKFKANEKNISLHFHHSIESLNINADKEK
                     LWRVFSNLIMNAIKFSHSGKSVYIHVEKYLNEIVVNVQDEGIGIPEKYYSKVFDMFTE
                     AKRKGTFGEQPFGIGLHICKQIVEAHNGKIWFISNEPIGTSFYVSLPIN"
     gene            complement(7046..8146)
                     /gene="dprA"
                     /locus_tag="PLY81_02215"
     CDS             complement(7046..8146)
                     /gene="dprA"
                     /locus_tag="PLY81_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNF29075.1"
                     /translation="MNNDLLYQIALTLVPNIGAVQAKLLIENFDSAEAIFKAKIKDLS
                     RVEGIGEIRAKAIKNFTEFKLAEEEILFTEKYKIECLCLKNEQYPKRLLHCYDAPTIL
                     YYKGNANLNNSKIISIIGTRTNTDYGKQITQEIIEHLQPFHALIVSGLAFGIDAIAHK
                     AALENNLATVGVLAHGLDSIYPTQHKKLAKEMLVNGGLLTECRKNIKADKFNFPQRNR
                     IVAGMADATIVVETAIKGGSMITAEMAYNYNKDVFAVPGKIYDNKSSGCLSLIQQNKA
                     ILLAKPQQIADELGWQTKTIKPPKQKELFVQLSSDEKIIINLLQLKETVAIDELFIQT
                     GLSSSAVAAALLNLELQNIVISLPGKIYKLIN"
     gene            complement(8170..>9856)
                     /locus_tag="PLY81_02220"
     CDS             complement(8170..>9856)
                     /locus_tag="PLY81_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF29076.1"
                     /translation="LKKHQLSVENYEWAKRKDTVYFRQYHLPYSINLAGIGKFDEALE
                     AINYFLSIPKISDKSIKAANYRKSCYQFALSYSTKHIDTSYTFIPENLGDSINTETSE
                     YYPSVTIDDSLLVFSRRGQGMREDFMQSKLDSNGYRKATFINGDINLEPSKGALTISA
                     DGEWMIFAGNFGREKGFGSFDLYISFYTPEGWSDPENLGPNINTEYWESSPSLSPDKR
                     VLYFSSNRYGGVGGKDLYFSERMANGKFGPAVNMGKGINSESDEHSLFIHVDNQTLYY
                     SSNGLPGYGGSDLYVLRKQADDTWSEPENLGYPVNTIDDEGYFAVSADGLTAYFASDR
                     ADSRGGLDLYKFKLRNNIRPAKTLYVKGKVYDLHTNKGLPCTIELIDNETGLALMKIQ
                     TDELGEYFITLPIGKNYTFSVNRKGYLFYNELYEMKNMVADSVYKKDIALQPVALHTS
                     IAFKNIQFEHNSYKLLPVSLIELDKLFQLLKDNPTLQVEIGGHTDNSGNTADNLKLST
                     NRAKAVVDYLVSKGIAINRLSYKGFGESKPIADNATAEGRAINRRTTFTFTQM"
BASE COUNT         3230 a         1539 c         1388 g         3699 t
ORIGIN      
        1 aaagcagatg aaaatgaaaa aacacctata ggcgtactgc ttaccatttt tgctgttgta
       61 attatgtttt gggctgtatt taaacaaaat ggcaccgcat taaacacttg ggcagataga
      121 tacacagatc gttctgtatc gggcaccaca gaaaaagttt ttaaagcagt acaatttaca
      181 caaaccttaa agtaccaaaa agattctgtg ccattatatg actctatgtt taggcttcaa
      241 aaacaaaatg gagtagtaat aaaagagttt aattacccag tttattttag aaatgacatg
      301 agaagtaaaa tgcaaactga aggtggcgaa ctaagcattt gggctactaa tttaagccaa
      361 agtattaacc ctgcttgggt aattattctt acaccgttaa tagtagcatt ttttacattt
      421 ttaagaggta gaaaaaaaga acctagtaca cctactaaga ttgcatttgg attatttgtt
      481 tctgcacttt ctgttttagt tatgattgca gcagtaaaag ctggtaataa tggggcagaa
      541 aaagtaagtg tttggtggtt aattggtagt tatggagttg ttaccattgg tgaactttta
      601 ctaagcccaa tgggtttatc tttagtatct aaactaagcc ctgtgcgtat tacttcttta
      661 atgatgggtg gatggttttt atctacttca ataggtaata aactatctgg tgttttagca
      721 agtatgtggg ataagtacga tgataaagca aactttttct ggctaaattt ctttttgtta
      781 ttattctctg catccattat gtttttaatg cttaagaggc ttaataaagt tttaaaagtt
      841 agaggagtta aataaatatt ttaatcaaaa ttttattgtt tgccagcctt tgtgctggca
      901 tttttttatt aactaaagaa attatttaaa ttactaaatg accatacttt ttttcatcaa
      961 attcatattt aaatcttcta tggctccata aatagttatc tggatgttgt ttcactgttt
     1021 cttctataaa tgcaactagt tgttttgtaa tagtgccttc tagaaattct tttggtgttg
     1081 ttgtaaccaa tttagcttct aatttgtagt agcctctttt tacttttgtc atctctataa
     1141 aagctatagc tgtattcata cgtattgctc ctttttctgg ccctgtatga aatggtgtca
     1201 ttctaccaaa aaaaggaaac caaacagcat tggttggtat tggtggattt tgatctgcaa
     1261 taagcccaat agaataaacg cttttgctat gcttaggaaa ttctgttcta aattcggatg
     1321 caggaaaaag aattgtacca aatttctttc tcattattac cattaccttt tctaaatttt
     1381 tgttagaaag ccccatataa acacctaaga aaggatattt aagattggct gcacaaccta
     1441 aattcaaaaa ttcggaatta aaaaaatgac cagataataa ttgaacactt tgccctgttg
     1501 aatacaaatc atttactact ttaaaatttg taatacatcg tttattaaac tcttttctag
     1561 aaatagttaa gagctttata ctttccacaa aagaatctat taaattatga taaaaagctt
     1621 ttgaaatttt aattctttct ctttcagttt tatcaggaaa tgcaatttgt aaattattgg
     1681 caactacatt tcttctatac ctaattacat aatatgctaa taagtaaaaa caatcgctca
     1741 aaaaatacat taccctccaa ggtagtaatg ataataggta aaataatgcg tagtagaaat
     1801 agaacatgtt gttgtaaaat aacaaaagta aacaatagct gcataaaaaa ttataactaa
     1861 taatccgtag ctttttaatt ttttatcaac aaataaaaaa attaaaggct ttggtagcaa
     1921 ctttgttgaa tgaaatttct tgtaatacgt ttctcctcta ttggagatat tgtgcttaca
     1981 acaccagttg taagatgctt aaaacaacaa gtaacaaatt cagagataca ttttttaaca
     2041 aaggcctcat tttctactat tgttgaaaat aattcttaca tagataaagt tgttttatta
     2101 aacaataatt gggatgagac tatactgcaa ctaaaacagg aaaattatga ttatattatt
     2161 gacttacatc ataatttaag aacactaaga ataaagaaag ctttaaaaat aaaatcgttc
     2221 tcctataata aactgaatat tcaaaaatgg ttgctcaccg cttttaaaat taatacacta
     2281 ccacccatac atattgtgga taggtatata gaaaccgtaa aaaaaatagg agtaattaac
     2341 gatggtaaag gattagatta ttttatacct gaaaaagatt gcattaaaca aaatgatata
     2401 cctgcacaac accagtttgg atatataggt attgtaattg gtgcagcatt aaataccaaa
     2461 aaaatgcctg taaacaaact gatagattta tgcagtaaaa tagattttcc tattattctt
     2521 ttaggaggta aagaagacaa agaaaatgga gatacaattg cagcaataga tacaataaaa
     2581 atatacaatg cttgcggtaa gtttaacctt aatgaaagtg ctgatttagt aagaaaagct
     2641 aaactaatta taacacacga tactggatta atgcatatag cagcagcatt taaaaaaccc
     2701 attctttctg tttggggtaa tacaattccg agttttggaa tgtcgcctta ttttggaaac
     2761 aaccaaatta ccaatttaca ttatgaagta aaacttggat gccgcccatg tagtaaaatt
     2821 ggttttaaca aatgtcctaa aaaacatttt aaatgtatgg aacaacaaga tgtgtctcaa
     2881 attgcaaaag atgcattaaa gttagtgtag aaagtatata aattgaattg ttatcttctg
     2941 ctggcaatta ataaattcaa atctgtgtat tttaaatcaa atttttgact gatgtagaaa
     3001 tctgttaagg ctcctttaaa caaataaata ccttctctta tattaaagtt gtgccaaata
     3061 atttcgtcta aacctccttc gttacttatt tgtaaaataa ggtgcataat tacattacta
     3121 attgcttggc ttgcagttct tgcaaaacca cttggtatgt ttggtacaca ataatgaatt
     3181 acatcgtatt ttaaaaaggt tgggttttcg tggctggtta gttggcttgt ttcaaaacac
     3241 ccacctctat caatagcaac atctataatt atgcttccct tacgcatatt ggcaaccatt
     3301 tcttcggtaa ctacaactgg cgaatggcca taagcattgc ttaaagcccc aacagccact
     3361 tcgcaggttt ttaattgttt tgctaaaatt ttaggttcaa gaacacttgt ccaaattctt
     3421 tgtcctaaat tattttgtag ttgttttagc ttgtaaacat tattatgaaa aaccttaaca
     3481 cttgctccta aagccaaggc atttctagtt gctacttcac ctacaattcc agctccaatt
     3541 attacaactt tagtaggtgg aataccgcta atactaccta acaaaactcc tttaccttta
     3601 ttaaagctac ttaaatattg cccagcaatt agcattactg ctccacctgc aatttcactc
     3661 atacttctta caattggaaa agtgccacta tcatctttta aattatcaaa gctaattgca
     3721 gtaacttttt tctccattaa ttttttaact gttgctttat ttaaagcaga atagtgaata
     3781 ggagatataa tagtttgccc attttgaata agaggtaaat cttcttgtac gggtggtgca
     3841 gtttttatta aaaccggtgc tttaaaaact tcagttctgt cgtaagtaat ggttgcccct
     3901 gcttcgccat attctttatc tgcatagtta catccttctc ctgctttagt ttctaccata
     3961 accgtatggc cattacttac caacacgcct acagcatcag gcgagagagc taccctgttt
     4021 tcttgaaaag caatttcttt gggtatgcct atgtgtagtt ttgcaccatc tggttttatg
     4081 tccaaggttt cttctagtgt ttcgtaacta aaagaagtgg atataacagg tttagaagca
     4141 gccataactg atttattaat tattgcctaa agttagttat agtaatttag tttaaatgaa
     4201 atagtttttt tatgctgcac aaacaagcgg taatcttatt tttttgtaaa ccttaaagta
     4261 ttaataaggt gttttacatc ttctaacaaa aagttgttta caatcttcaa tgagtcttca
     4321 ttaggtgtgg catcaaaata taatgcccct cttaaaaaat gagttacact atccgttaaa
     4381 aaaaattgat ttgcagttgc aacatctccg cctactttaa aataaatacc atgtacattg
     4441 ttaggtgtaa taattaatga atcgtctata gatgttgctt tttgtgtatg cttgttggtt
     4501 aatttaaaag catcctctac taaatcatta aaattattat ttgagattag tttatagctt
     4561 acataaattc ttccattaaa ctgaggaaaa tctatattta tccaccaagg gttttctgtt
     4621 tttgctccaa aaaataaaga atctttaacc acttttgcat aaaccgggta ttcaaaacta
     4681 taaggatagt ctttcatttg aaaagtggta tatttttttt cgggaaaatt tattttaaaa
     4741 tagccttttg gttttggagt ataaacacta ttacaagcca atagtgtagt tattattaat
     4801 agaaaacaag cactgaaatt taaaaatgaa aatttatttt tcatacccat aaattatttt
     4861 ttgtgtggaa agaatgcaaa gtaatactat taattaatag gcagggaaac ataaaatgat
     4921 gtaccaatag gttcattaga aataaaccat attttaccat tgtgtgcttc tactatttgt
     4981 ttacaaatat gtaagccaat tccaaaaggt tgttctccaa atgttccttt acgctttgcc
     5041 tctgtaaaca tatcaaacac tttactataa tatttctcag gtattcctat accttcatct
     5101 tgaacattta ctacaatttc gttcaaatat ttttctacat gtatataaac agattttcct
     5161 gaatggctaa attttattgc attcattatt aaattagaga aaaccctcca taatttttct
     5221 ttatctgcat ttatatttaa actttctatg gaatgatgaa aatgaagact tatgtttttt
     5281 tcatttgctt taaactttaa taattcggta cattgcttta ataattcaat aatatttatt
     5341 tcttccttta ctaattcttt ttgttctagg ttagagctag tgttaagcaa ttcagtaatc
     5401 atacttaatg aattattgct tgacgattgt attaaagtta gcatttcctt tttttcattt
     5461 tcatcaatag tactatcatt caataaaata tctgttatac ctgttatacc tgctaagggg
     5521 cttcttaagt catgggccat taccttcatt acacgtgcaa aatttttatt actttgttca
     5581 aattgttgta aggtactttt taattgtaaa ttatgaagtg tggtttggtt tgcagtaatt
     5641 tttatttgct ttaaatatct taaaattaat attataataa caaataataa tgccattact
     5701 attgaaaata caataatgta aatggtttta atttcattct ttttttcgaa attttttaat
     5761 aaatattcgc ttttaatttt atctacttct ttgcctatac taaaattatt taaatcataa
     5821 gcaatttgat ttactgagtc ttgcacatga aaaaaacttt caaatgcttt aattgccttt
     5881 tctaattggc ctgattggta ataataatta taatataata atcgttgctt acttctaatg
     5941 taaccaatgg taggatgtga atctgaattt atcaacctat cagaaaaatc taaaatatct
     6001 tttgcttttg catattgctt tttacttaaa taaacatcca caagttttaa ataggcagtt
     6061 tgtaaatcaa tagaatcata accgggtttt gaattaatat ggatggattt atttaaataa
     6121 taaatagcag agtctggttg cttattataa gcaaatgctc cacctaaatt tccataataa
     6181 attcctaatg ctaattctct tttctgattg tttatttcat gtatagattc ttgattttga
     6241 ataaatttaa tgccttgttg gtaaaatata atggcactat cataagctcc taatttttca
     6301 taacataaag ctttagtgtt taacgttgat tgaattcgat gaaaatataa aaaataagcg
     6361 gtgttatttt tacatttttc ttctctatct atactaatat ttaagtaatc tagggctttt
     6421 ctgtaatctt ttttggagta ataaaaattc cacaacttat tgtaatactt gctataatta
     6481 caagtatcgg tttgggcatc taaaattaat ttagctttat aatattcttt taatgcctct
     6541 tcgtattttc ttgcagcaaa aaaatattct cctttagcaa ataaagcatc tgtatagtac
     6601 ttagagtaaa ctgtagttag tttattattt tctaaataat taagtaatgc ctctgcatta
     6661 gtgtaattct cataatagtt attggtataa tgttctttta aataaagtaa ataataatat
     6721 aaaactttag tgttgtgcga ttgtgggtag attttcttta agctatctaa agtataattt
     6781 aaaaaaacag aatcggttaa gtgaaaagta ataaatttat tctctagctc ttcgtttgct
     6841 ttaaaattga ttggctctgg tttgtgggag taattacaac tattaactag caatagtgct
     6901 gctattgcca acagataaaa aaataaaatt ggatatttat agttcttaca cttaccaaat
     6961 agcatcatgt cttaaagagt gaaatttcaa atttagccgt tgcaatgtaa gcaaataatt
     7021 aatatgaaaa attaaaatta tataatcagt taattaactt gtaaatttta ccaggtaaac
     7081 taattactat gttttgtaac tctaaattta gtaatgctgc agctacagca ctactactta
     7141 gcccagtttg aataaataat tcatctatag ctactgtttc ttttaattgt agaagattaa
     7201 ttataatttt ttcatctgat gataattgaa caaataattc tttttgtttt ggaggtttta
     7261 tggttttggt ttgccaaccc aactcatctg caatttgttg tggcttggct aataaaatag
     7321 ctttgttttg ttgaattaaa ctaaggcatc cactactttt attatcataa attttacctg
     7381 gtacagcaaa cacatcttta ttataattat aggccatttc tgctgttatc atactaccac
     7441 ctttaatagc tgtttcaact actattgtgg catctgccat gcctgctaca attctatttc
     7501 tttgaggaaa attaaattta tcggctttta tattttttct acattccgtt aataagccac
     7561 catttaccaa catttctttt gcaagttttt tgtgttgtgt tgggtaaata ctatctaagc
     7621 catgagctaa tacaccaaca gttgctaaat tattttctaa tgcagcttta tgtgcaatgg
     7681 catctattcc aaatgctaaa ccacttacaa taagtgcatg aaaaggttgt aaatgttcta
     7741 taatttcttg tgttatttgt ttaccataat ctgtatttgt tcttgtacca attatactaa
     7801 ttattttgga gttgtttaaa ttggcattcc ctttataata taaaattgtt ggagcatcgt
     7861 aacaatgtaa taacctttta ggatactgct cattttttaa acataaacat tcaattttat
     7921 atttttctgt aaacaatatc tcttcttctg caagtttaaa ttcggtaaaa tttttgatag
     7981 cttttgctct tatttctcca ataccttcta ccctacttaa atctttaatt tttgctttaa
     8041 aaattgcttc agcactatca aaattttcta tcaataactt tgcttgaact gctccaatat
     8101 tgggtacaag tgttaatgca atttggtata ataaatcgtt attcattgaa taatgatggt
     8161 tacatttttt tacatttgtg taaatgtaaa agtagttctt ctgtttattg ctcttccctc
     8221 tgctgtagca ttatctgcaa taggttttga ttcgccaaaa cctttataag atagtctgtt
     8281 tatagcaatt cctttactca ctaaataatc tactacagct ttagctctgt tggtacttaa
     8341 ctttaaatta tctgcagtgt tgccgctatt atcagtatgt cctccaattt ctacttgtag
     8401 tgttgggtta tcttttaaaa gttgaaatag cttatctaat tctatcaagc ttactggtaa
     8461 taatttataa gagttatgtt caaactgaat atttttaaat gcaatactgg tatgtagagc
     8521 aactggttgt agggcaatat cttttttata tacactatct gcaaccatat ttttcatttc
     8581 atataattca ttataaaata aatatccttt tctgtttaca gagaaggtat agtttttacc
     8641 aattggtaat gttataaaat attctcccaa ttcatctgtt tgaatcttca ttaatgccaa
     8701 acctgtttca ttatctatta attctatggt acagggcaat cctttgtttg tatgcaaatc
     8761 gtacactttg ccttttacgt ataaagtttt tgcaggtctt atattatttc ttaatttgaa
     8821 tttatataaa tctaatccgc ctctcgaatc tgctctatca cttgcaaagt atgcagttaa
     8881 accatcggca cttacagcaa aatatccttc atcatcaatg gtattaacag ggtagcctaa
     8941 gttttcaggt tcgctccaag tgtcatctgc ctgttttctt aaaacatata aatcactacc
     9001 accatagcct ggcaaaccat tgctagaata atataatgtt tggttatcta catgtatgaa
     9061 aagagaatgt tcatctgatt cggagttaat tccttttccc atgttaactg caggtccaaa
     9121 tttgccgttt gccattcttt cgctaaagta taaatctttg cctcctacgc caccgtacct
     9181 attactgcta aaataaagta ctcttttatc tgggctaagg cttgggctgc tctcccaata
     9241 ctctgtatta atatttggcc ctaagttttc cggatcactc catccctctg gtgtgtaaaa
     9301 agaaatgtac aaatcaaaac ttccaaagcc tttttctctt ccaaaatttc ctgcaaaaat
     9361 catccactcg ccatctgccg atatagttaa agcacctttg gagggttcta aattaatatc
     9421 tccgttaata aaagttgctt tgcgatagcc attactatct aatttacttt gcataaaatc
     9481 ttctctcata ccttgccctc ttctggagaa aactaataag ctatcatcaa ttgtaacact
     9541 tggataatat tcacttgttt cggtatttat actatctcct aaattttctg gaataaatgt
     9601 atagctggta tcaatatgtt tggtagaata ggaaagtgca aattgataac aactttttct
     9661 gtagtttgct gctttaatac ttttgtcgct aatttttgga atacttaaaa aataatttat
     9721 tgcttcaaga gcttcatcaa acttaccaat tcctgcaaga ttaatagaat agggcaaatg
     9781 gtattgcctg aagtaaacag tatcttttcg tttagcccat tcataattct ctacagatag
     9841 ttgatgcttt tttaac
//
LOCUS       DAOQHP010000036         9827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3509_length_9827_cov_3.790524, whole genome shotgun sequence.
ACCESSION   DAOQHP010000036 DAOQHP010000000
VERSION     DAOQHP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_9827_cov_3.790524"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLY81_02225"
     CDS             <1..623
                     /locus_tag="PLY81_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF29077.1"
                     /translation="NEESKLIAKKLSLPQINASIINNESQQNDKLKEIAELDNQIAVQ
                     KSIFMQALQTLKSEIQAWEYKYVIKAPVAGSVAFTGFYQENQEIKNGQSLFVVQPTNT
                     QYFLEAQILQYNFGKVKLGQQVLLKFEAYPYEQYGSVTGKIDFISTIPTDSGYLAKIV
                     LPDGLISNYKKPLQYHTGLKAQADIVTEDMRLLERFYYNIRKQLTR"
     gene            complement(762..1712)
                     /locus_tag="PLY81_02230"
     CDS             complement(762..1712)
                     /locus_tag="PLY81_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNF29078.1"
                     /translation="MKNFKAFIQLIRLPNLIFIAITQIIFLYCIEMPLFNEASVINNV
                     RGIYFVALVTASVFIAAAGNIINDYFDLNIDQINKPHKVVVEKIIKRRWVILWHILLS
                     LTGIVLGFYVDASTPVRFLGIANSACVVLLFFYSASLKKKFLIGNILISLLTSWVILV
                     ITYCETNHLFDVFRKDSSLQVDKLSRLTFLYAGFAFIISLIREVIKDMEDMEGDRKFG
                     CKTLPIVLGLNATKVFVAVWLIVLIAALVIIQFYVLQFGWVFWLSALYCVLFIITPLI
                     WILLKLFKAHTAQEFHKLSSVVKIVMFTGILSMLFFRLYA"
     gene            complement(1729..2250)
                     /locus_tag="PLY81_02235"
     CDS             complement(1729..2250)
                     /locus_tag="PLY81_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNF29079.1"
                     /translation="MNVLEQFSKIKTFVFDMDGVLTDGSLTLLPDGAMCRRMNIKDGY
                     ALQLAVKKQYNIVVISGGTSPESEMRLRKLGITNIFMQVPDKATLLKEFLEEKKILSE
                     QVLFMGDDVPDFNAMQIVGLPCCPTDAAIEIKEIAHYISPLKGGEGCVRDVIEKVLKL
                     QHNWKDDFFIQSK"
     gene            complement(2281..2520)
                     /gene="iscX"
                     /locus_tag="PLY81_02240"
     CDS             complement(2281..2520)
                     /gene="iscX"
                     /locus_tag="PLY81_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNF29080.1"
                     /translation="MNFEPPIHWNDYEDIALKLYERFGDDFSESKIYRIRFTDLLEWV
                     LQIPGFVGTREQSNEGHLEMIQSSWVYEWRDNQPK"
     gene            complement(2623..3201)
                     /locus_tag="PLY81_02245"
     CDS             complement(2623..3201)
                     /locus_tag="PLY81_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNF29081.1"
                     /translation="MKEILLIDNYDSFTYNLVHLIEKITSLKPIVYLNDKIDLEAVNQ
                     YETIILSPGPGIPKEAGIMLPLIKDYAKTKKILGVCLGHQAIAEAFGASLINLPKVFH
                     GVATECFVDKTNNSLFQNITQPFMVARYHSWAVSKNNFPENLEIIAEDKDGIVMALQH
                     KIYNVKGIQFHPESILTPLGENILRNWIEDIK"
     gene            3302..3589
                     /locus_tag="PLY81_02250"
     CDS             3302..3589
                     /locus_tag="PLY81_02250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF29082.1"
                     /translation="MEILHNTSKPISCFYIKEGDVVLAKIDYKVEEDNTIFILHTEVS
                     EKLKGMNIGNKLLQKVADLARSEHRKIVPLCSFALAMFNKKEEYKDVWKQA"
     gene            3624..4214
                     /locus_tag="PLY81_02255"
     CDS             3624..4214
                     /locus_tag="PLY81_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF29083.1"
                     /translation="MGRIKTTYNLSKKEIILEKAAQLIKEKGFKAASMRDLAEIVGVE
                     AASLYNHIKNKNELLYEICLNVADLYNQQIALIETEEVSNLEKIEKLLRFHIQCMVIN
                     FEKVFVSDREWKHLSEPYLSNFQMQRRNYRKRFAALINDGIYKNEIKKIDATTAVLIM
                     LYAVSGIESWHRSKQKISGIELEENMMAILVAGLKK"
     gene            4253..5329
                     /gene="paaK"
                     /locus_tag="PLY81_02260"
     CDS             4253..5329
                     /gene="paaK"
                     /locus_tag="PLY81_02260"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HNF29084.1"
                     /translation="MSLHFYPLTIKQVNQETANCVSVLFDVPENLQATFAYKHGQNIT
                     VKTTINQQEVRRSYSLCSTPLSNEFRIAIKKLDGGLFSTFANEGLKEGDVLEVMPATG
                     RFFTEINAHHQKNYVAFAAGSGITPIISIIKTILLAEPKSTVTLIYGNKNVSSIIFKE
                     ELDAIKNNFLQRFQLLHILSQEGTDAPVFSGRINEEKLQQLSAIINYNKVDNFFVCGP
                     EAMIFTIKNYLEQLGIENNKIHFEIFVSSTPNLNKNSSKKIIDSKEATSKIVIKADGR
                     SFDFDLPFNSASILDGALKHGADLPFACKGGMCCTCKAKVLEGKVEMDVSWGLGADEI
                     EQGYILTCQSHPITSKVVVDYDIK"
     gene            complement(5456..6718)
                     /locus_tag="PLY81_02265"
     CDS             complement(5456..6718)
                     /locus_tag="PLY81_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNF29085.1"
                     /translation="MFALVDCNNFYASCERVFRPDLNEKPIVVLSNNDGCVIARSNEA
                     KALGIPMGAPAFEYEKIFQQNNIQVFSANFALYGDMSQRVMNILSEYTPNIEMYSIDE
                     CFLQLTGFDAHFNLQQYGNTMCKKVLQYTGIPISVGIATTKSLSKVANKIAKKYTKQT
                     GGVYIIDNEEKRLKALKWLKIEDVWGIGRQHSKRLHAIGITTAFAFTQLDNAYVQKQF
                     SIVELRLKQDLQGIPTLGLEEVQAKKSIATTRTFENNYTTVEELIERISTFAVSCAEK
                     LRKQNACCNSLMVFIHSNRHRKDLPQYNRSIVIKLPFATNSTIELAKFAVQALKLIFK
                     QGYAYKKAGVIVQDFVTENNIQQTLFENRNPKHISLMKAIDQLNVNFGQQKIRLASQD
                     QKRVWKMKQEKLSPRYTTNLADIITVHT"
     gene            complement(6719..7201)
                     /gene="umuD"
                     /locus_tag="PLY81_02270"
     CDS             complement(6719..7201)
                     /gene="umuD"
                     /locus_tag="PLY81_02270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNF29086.1"
                     /translation="MFYKPYNLPITLPPLKLHTSKTIDIYSALSETALELPFVGQNIS
                     AGFPSPALDFTDLGIDLNKHLIKHPSATFYGRAKGQSLKNAGINDGDLLIIDRSVEPI
                     NGKIAVCYIDGEFTAKRIKKTKNELWLMPENEDYKPIKIEEENNLIIWGIVTHVIKAV
                     "
     gene            complement(7360..7433)
                     /locus_tag="PLY81_02275"
     tRNA            complement(7360..7433)
                     /locus_tag="PLY81_02275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7397..7399),aa:Pro,seq:ggg)
     gene            complement(7501..8460)
                     /locus_tag="PLY81_02280"
     CDS             complement(7501..8460)
                     /locus_tag="PLY81_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF29087.1"
                     /translation="MRKIERIIINLQPVAFIIKKGKEIKPPGFSGLAIYDVIMFLFTH
                     LNKVGLNERAAAISFNFIMAIPAACIFIFTLVPYIPIAKHFNAELLKLTADITPNQNT
                     YYFIKDFLNDFFNKPRTGLLSFGFLLVIFYASNAMMGIIRAFDKSVKLQKQYFFQLRI
                     RAIKLTTLLVLLVIATILVLIGHEQLAALIKSIFKTKSKLTWWGSVRWIIILALVFYS
                     IALIYKYAPSINKRWKLITPGSLLATCLILITTILFSFWVNNFSSYNKIYGSIGTVLI
                     IMILIYINSLILLIGFELNVSIMQLKLNAEAREKNEQLQSKPS"
     gene            complement(8496..>9827)
                     /locus_tag="PLY81_02285"
     CDS             complement(8496..>9827)
                     /locus_tag="PLY81_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNF29088.1"
                     /translation="TPGHKDFAEDTYRTLTAVDSVILVIDSVNGVEDQTRRLMEVCRM
                     RDTPVIVFINKMDRDGKNRFDLLEEIEKELKITLHPMTWPINSGKDFKGVYNIHHKNL
                     RLFSANTKASEEDTIAIENLNEEILDKKIGERDAIQLREDAELIEGVYGILNEKEYLC
                     GKIAPVFFGSAVNNFGVKEMLETFITIAPTPLCRQTNTREVCPGEEKFSGFIFKIHAN
                     LDPKHRDRIAFMRVCSGKFERNKYYNHVRLEKTMRFSNPYSFLARSKDTIEDAYPGDV
                     IGLFDTGNFKIGDTLTEGEKFYYTGIPTFSPEIFKELVNKDPMKTKQLEKGIQQLTDE
                     GVAQLFTQFGGNKKIIGCVGELQFEVIQYRLLQEYGAACEFRVLPFYKACWLTSKDKQ
                     KLEDFLRFKQQNSGEDKDGNPIYLAQSEWFLNTEINNNPDIEFHFSSEVQK"
BASE COUNT         3417 a         1496 c         1441 g         3473 t
ORIGIN      
        1 ggaatgaaga aagtaaactg attgctaaaa aattatcgtt accgcaaata aacgcttcta
       61 ttattaacaa tgaaagtcaa caaaacgata aactaaaaga aatagcagaa ttagacaatc
      121 aaattgcagt gcagaagagt atttttatgc aagctttgca aacacttaaa tccgaaatac
      181 aagcgtggga atataagtat gtaataaaag cccctgttgc aggcagtgta gcatttacgg
      241 ggttttacca agaaaatcag gaaataaaaa acggacaatc tttatttgtt gttcagccta
      301 ccaatacaca atatttttta gaagcacaaa tactacagta taattttggc aaagtaaaac
      361 tagggcagca agtgttgtta aaatttgagg cttaccctta cgaacagtac ggctctgtaa
      421 caggcaaaat agattttata agcacaatcc caacagatag tggttattta gctaaaatag
      481 ttttacccga tggattaata agtaattaca aaaagccact gcaataccat acaggcttaa
      541 aagcacaagc agatatagta acagaagata tgcgattgtt agaaaggttt tattataaca
      601 ttcgtaaaca acttactcga tagtataatc cttgcttccc atttacttaa ctattgacaa
      661 tcaaaagttg aaattttaat tatggagatt gttgatttta acattacttg ttgtgtaggt
      721 tctatacaaa tagaataatc tataaaattt aatttatcta tctacgcata caatctaaaa
      781 aaaagcatac ttaaaattcc ggtaaacatt actattttaa ctacactgct tagtttatga
      841 aactcttgtg cagtatgggc tttaaagagt tttaataaaa tccaaattaa aggagttatt
      901 ataaataata cacaatataa agcacttagc caaaataccc aaccaaattg caacacgtaa
      961 aattgtatta ttaccaatgc tgcaattaat actattaacc aaacagcaac aaacactttt
     1021 gttgcattta aaccaagtac aattggtagt gttttacaac caaattttct gtcgccttcc
     1081 atatcttcca tatcttttat aacttctcta attaatgaaa ttataaaagc aaaacctgca
     1141 tataaaaaag ttagtctaga aagtttatca acttgtaaac tgctatcttt tctaaacaca
     1201 tcaaataaat gattggtttc gcaataggtt ataactaaaa taacccatga tgttagtaaa
     1261 gaaattagta tgttgccaat taaaaatttt tttttaagag aggcagagta aaaaaacagt
     1321 agtacaacac aagcagaatt tgcaattcct aaaaatctaa caggtgttga agcatctaca
     1381 taaaagccta atacaatgcc tgttaaactt agtaaaatat gccatagaat aacccacctt
     1441 ctttttatta ttttttctac aacaactttg tgtggtttat taatttggtc aatattcaaa
     1501 tcaaaataat cgttaatgat gttacctgca gcagctataa aaactgatgc tgtaaccaat
     1561 gcaacaaaat aaatacctct aacattattg attacactgg cttcattaaa caaaggcatt
     1621 tctatacaat acaaaaaaat aatttgtgta attgcaataa atattaaatt aggcaatcgt
     1681 attagttgta tgaatgcttt aaagtttttc aattaaaaca atttaaattt atttagattg
     1741 aataaagaaa tcatccttcc agttgtgctg caactttaat actttttcta ttacatctct
     1801 cacacaacct tctcctcctt ttagtggaga aatataatgt gctatttctt taatttcaat
     1861 tgctgcatca gtaggacaac atggtaagcc aacaatttgc attgcattaa aatctggcac
     1921 atcatcaccc ataaataaaa cctgttcgga taatatcttt ttttcttcta aaaattcttt
     1981 aagcaatgta gccttatctg gcacttgcat aaaaatattt gttatgccta attttcttaa
     2041 tctcatttcc gactctggtg aggtaccacc acttattacc acaatattgt attgtttttt
     2101 tacagctaat tgtaaagcat aaccatcttt aatattcatt cttctgcaca tggcaccatc
     2161 aggcaataat gttaaactgc catctgttag tacgccatcc atatcaaaaa caaatgtttt
     2221 aattttacta aattgttcaa gcacattcat tgtaaaaaat agatttgaaa ataaaattat
     2281 ttatttaggt tggttatctc tccattcata tacccaacta ctttgtatca tttctaaatg
     2341 tccttcatta ctttgctcac gtgtgcctac aaagccagga atttgtaaaa cccattctaa
     2401 taaatctgta aatcttattc tgtaaatttt actttcgcta aaatcgtcgc caaaacgttc
     2461 atatagttta agagcaatat cttcataatc gttccaatga ataggtggtt caaaattcat
     2521 tcttcaaaaa ttttaaaatt ataaaatgca aatatgcttt taattcggca tttaatagct
     2581 aaacaatgga atatactttt ttaatataaa aatctacttg ctttatttaa tgtcttcaat
     2641 ccaatttctt aaaatatttt cacctaaagg agttaaaata ctttctggat gaaactgaat
     2701 gcctttaaca ttataaattt tgtgctgtaa tgccattaca attccatctt tatcttctgc
     2761 aataatttct aaattttcag gaaaattgtt tttacttact gcccatgaat gataacgagc
     2821 aaccatgaaa ggttgtgtaa tattttggaa taaactgttg tttgttttat ctacaaaaca
     2881 ttctgttgca acgccatgaa atacttttgg caaatttatt aaacttgctc caaatgcttc
     2941 tgcaattgcc tgatgcccta aacaaactcc taaaattttt tttgttttag cataatcttt
     3001 tattaatggt agcattatac cagcttcttt aggaattcca ggtcctggag ataaaataat
     3061 ggtttcgtat tgatttactg cttctaaatc aattttatca tttaaatata ctatgggctt
     3121 aagtgaagta attttttcaa ttaaatgcac taggttgtat gtaaaagagt cgtaattatc
     3181 tattagcaaa atttctttca tggcttaaag cttgtatgcc caataattta tcttcaataa
     3241 tacaaaatca aagtttaaaa actaaacaat tttaaagtaa ctttactgta aacaatttat
     3301 aatggaaatt ttacacaaca caagtaagcc aataagctgt ttttatataa aagaaggaga
     3361 tgttgtttta gctaagattg attataaggt tgaagaggat aatactattt ttattcttca
     3421 taccgaagtg agtgaaaaat taaaaggtat gaatattggt aataagctac ttcaaaaagt
     3481 agcagatttg gccagaagtg aacacaggaa aattgtaccc ttatgtagtt ttgcattggc
     3541 aatgtttaat aaaaaagagg aatataaaga tgtgtggaaa caagcttaat ttataataag
     3601 cttttatata tttttgtaat aatatgggta gaattaaaac tacatacaac ctaagcaaaa
     3661 aagaaattat acttgaaaaa gcagcacagt taataaaaga aaaaggcttt aaggctgcaa
     3721 gcatgagaga tttggctgaa attgttggtg tagaagcagc cagcttatat aaccatataa
     3781 agaataagaa tgaactattg tatgaaatat gtttaaatgt agccgattta tacaatcagc
     3841 aaattgcttt aattgaaaca gaggaagtaa gcaatttaga aaaaattgaa aaattattac
     3901 gttttcatat tcaatgcatg gttataaatt ttgaaaaagt atttgtaagc gatagagaat
     3961 ggaaacattt atctgaacct tatttatcta actttcaaat gcaaagaaga aattacagaa
     4021 aacgatttgc tgcattaatt aatgatggta tatacaaaaa tgaaataaaa aaaattgatg
     4081 ctactaccgc agtgttaatt atgttgtatg ctgtaagtgg cattgaaagc tggcatagaa
     4141 gtaaacaaaa aatttcaggt attgaattag aagaaaatat gatggcaatt ttagtagctg
     4201 gcttaaaaaa ataaactttt aatatcaacc aataatattt ataatttaaa atatgtcttt
     4261 acacttttat cctttaacta ttaagcaagt aaatcaagaa actgccaatt gtgtttcggt
     4321 tttatttgat gttccagaaa atttacaagc aacatttgct tacaagcatg ggcaaaatat
     4381 aactgtaaaa actactatta atcaacaaga ggtaagaagg tcttattcct tatgctctac
     4441 gccattaagt aatgaattta gaattgcaat taaaaaatta gatggtggtt tgttttcaac
     4501 ttttgccaat gaaggcttaa aggaaggaga tgttttagag gtaatgccgg caacaggcag
     4561 attttttaca gaaattaatg ctcatcatca aaaaaattat gttgcttttg ctgctggtag
     4621 tggtataacc ccaattattt ctatcattaa aactatttta ttagccgagc ctaaaagtac
     4681 tgtaacgctt atttatggta acaaaaatgt aagctctatt atttttaaag aagaattaga
     4741 tgctataaaa aataattttt tacagaggtt tcagttgttg catattttat cacaagaggg
     4801 tacagatgct cctgtttttt caggcagaat taatgaagaa aaactacaac aactttcggc
     4861 aattattaat tataataaag tagataattt ttttgtttgt ggaccggaag ctatgatttt
     4921 tactatcaaa aactatttag aacaattagg aattgaaaat aataaaattc atttcgaaat
     4981 ttttgtttct tcaactccaa atcttaataa aaacagttct aaaaaaataa tagatagtaa
     5041 ggaagccaca agtaaaattg taattaaagc cgatggtaga agttttgatt ttgatttacc
     5101 ctttaacagt gccagcattt tagatggagc tttaaaacat ggtgccgact tgccttttgc
     5161 ctgcaaagga ggtatgtgtt gtacttgtaa agcaaaagtg ttagaaggca aggtagaaat
     5221 ggatgtaagc tggggtttgg gtgcagatga aattgaacaa gggtatattt taacatgcca
     5281 aagtcatcct attacaagta aggtagtagt agattatgat ataaaatagt ttgaatttaa
     5341 atagctgcaa agtgttggta aggtggtgtt atactaggct attttacaaa aaattattaa
     5401 ccatattttg aatatttatt tatacttatt taattggttt aactatattc aaatttcaag
     5461 tatgcacagt tataatatct gctaaattgg ttgtatatcg tggcgatagt ttttcttgtt
     5521 tcattttcca aacacgcttt tgatcttgtg atgctaaacg aattttttgc tgaccaaaat
     5581 ttacattcaa ctggtctatt gctttcatta aagaaatgtg tttagggttt ctgttttcaa
     5641 ataatgtttg ttgtatatta ttttctgtta caaaatcttg tacaataaca ccagcttttt
     5701 tataagcata accttgttta aaaattaact ttaatgcttg tacggcaaac tttgctaatt
     5761 caatagtaga gtttgtagca aaaggtagtt taataacaat acttctgttg tattgtggca
     5821 agtcttttct atgcctattg ctatgtataa aaaccattaa tgagttacaa caagcatttt
     5881 gttttcttag tttttcggca caagaaacag caaaagtgga tatacgttct atcaattctt
     5941 ctactgtagt ataattattt tcgaaggtac gggtagtggc aatacttttt ttagcctgca
     6001 cttcttctaa accaagtgta gggatgcctt gtaaatcttg ttttaatcta agctctacaa
     6061 tagaaaattg tttttgtaca taagcgttat ctagctgtgt aaatgcaaaa gcagtagtta
     6121 tacctatggc atgtaagcgt ttgctatgtt gtcttccaat accccaaaca tcttctatct
     6181 ttagccattt taatgctttt aatctttttt cttcgttatc aataatatat acaccacctg
     6241 tttgcttggt atatttttta gcaattttat tagcaacttt agagagagat ttggtagtag
     6301 caatacctac actaatgggt atgcctgtat attgtaatac ttttttgcac attgtattgc
     6361 catactgttg caagttaaaa tgtgcatcaa atccggttag ttgtaaaaaa cattcatcaa
     6421 tactatacat ttcaatattg ggggtgtact cacttaatat attcattacc cgttggctca
     6481 tatctccata taaagcaaaa ttggctgaga atacttgtat attattttgt tgaaatattt
     6541 tttcatattc aaaagctggt gcacccattg gaatgccaag agctttggct tcattacttc
     6601 tggcaataac acaaccatcg ttgttagata atacaacaat aggcttctca ttcaaatctg
     6661 gccgaaaaac tctttcgcaa gaagcataga aattattaca atcaactaaa gcaaacattt
     6721 aaacagcttt tataacatgg gttacaatac cccaaataat taaattattt tcttcctcaa
     6781 tttttatagg tttataatct tcattttctg gcattagcca taactcattt tttgtttttt
     6841 taattcgctt ggcagtaaat tcaccatcaa tataacaaac agcaatttta ccatttattg
     6901 gttctacact tctgtcaatt attaataaat cgccatcgtt aatgcctgca ttttttaaag
     6961 attgcccttt agcacgccca taaaatgtag ctgatgggtg cttgattaaa tgcttgttta
     7021 agtctatacc caaatctgta aaatcaaggg caggagaggg aaaacctgca ctaatatttt
     7081 gccctacaaa tggtagttca agggctgttt cacttaaagc ggaatagata tctattgttt
     7141 tgcttgtatg tagttttaat ggcggcaatg ttataggtaa attgtatggt ttataaaaca
     7201 aattgctagt gaaaataaaa ttatttttta ataattgagt acatctgaat atatattttt
     7261 tgtgtactag tttaatggat tgattggaca gaaaaacaag tgcattttta aaaaaaggta
     7321 aggctttggc tataagccaa agcctttgtt gtatagttgt cgggtggact ggattcgaac
     7381 cagcgacccc ttgcacccca tgcaaatacg ctaccggact gcgccaccac ccgtaacttt
     7441 tttattgttt gcaaatatat gttctgaaaa atatttttta ataaataaac aagggtttat
     7501 ttatgagggt ttgctctgta attgttcatt tttttctctg gcttcggcat ttaattttag
     7561 ttgcataatg cttacattta attcgaagcc tattaataaa attaaagagt taatataaat
     7621 tagaatcata ataattaata cagtaccaat tgagccataa attttattgt acgatgaaaa
     7681 attatttacc caaaatgaaa ataaaatagt tgtaattaat attaaacaag tggcaagcag
     7741 agagccagga gtaattaatt tccaccgttt attgatagaa ggggcatatt tataaattaa
     7801 agcaatacta taaaaaacca atgctaaaat aattatccat cttacgctgc cccaccaagt
     7861 aagtttagat tttgttttaa aaatactttt tattaatgca gctaactgtt cgtgccctat
     7921 taaaactaaa atggtagcaa taactaataa aaccaataat gttgtaagtt ttattgccct
     7981 tattcttaac tgaaaaaaat attgtttttg tagttttact gatttatcaa aagctctaat
     8041 aatacccatc atggcattgg atgcataaaa tattactaat aaaaaaccaa aggatagtaa
     8101 acctgttctt ggtttattaa aaaaatcatt taaaaaatct tttataaaat agtaagtgtt
     8161 ttggttgggt gtaatatcgg ctgtaagttt tagcagctct gcattaaaat gtttagcaat
     8221 gggtatgtaa ggtactaaag taaaaataaa aatacaagca gccggtatag ccataataaa
     8281 attaaacgaa atggctgctg ctctttcatt taaaccaact ttattaaggt gggtaaataa
     8341 aaacataatt acatcataaa ttgctaaacc gctaaagcca ggtggcttta tttctttacc
     8401 ttttttaata ataaaagcaa caggttgtaa gtttattata attcgttcta tttttctcac
     8461 aataagtaaa ttaataaact aaagatagta taaacctatt tctgaacttc gctactaaaa
     8521 tgaaattcaa tatctgggtt gttatttatt tcggtgttta aaaaccactc gctttgggct
     8581 aaataaatag gattaccatc tttatcttct ccgctatttt gttgtttaaa ccttaaaaag
     8641 tcttcaagtt tttgcttgtc tttgcttgta agccaacaag ctttgtaaaa tggtagtact
     8701 ctaaattcgc aggctgcacc atactcttgt aatagcctgt attgaattac ttcaaactgt
     8761 aattctccta cacaccctat aattttttta ttaccgccaa actgtgtaaa taattgtgct
     8821 acaccttcgt cggttaattg ttgaattcct ttttcgagtt gtttggtttt catggggtct
     8881 ttattaacca attctttaaa tatttcagga gaaaatgtag gaatgccagt gtagtaaaat
     8941 ttttctcctt cggttaaagt atctccaatt ttaaaattac ctgtatcaaa taaacctatt
     9001 acatcgccag ggtaggcatc ttctatagtg tctttacttc ttgctaaaaa agagtagggg
     9061 ttgctaaaac gcatggtttt ctctaacctt acatggttgt agtatttgtt tctttcaaac
     9121 ttgccactgc aaactctcat aaatgcaatt ctatctctgt gtttagggtc taagttggca
     9181 tgaattttaa aaataaatcc actaaacttt tcttcaccag gacaaacttc tctagtgttt
     9241 gtttgtctgc aaagtggtgt aggggcaatg gtaataaagg tttcaagcat ttcttttaca
     9301 ccaaaattat taacagcact gccaaaaaat actggtgcaa tttttccgca taaatattct
     9361 ttttcattta atatgccata cacgccttct attaattctg catcttctct tagttgtata
     9421 gcatctcttt cgccaatttt tttatctaaa atttcttcat ttaaattttc aatagcaatg
     9481 gtatcttctt cactggcttt tgtattggca gaaaataatc ttaaattttt gtgatgaata
     9541 ttatatacac ctttaaaatc tttgccacta tttattggcc atgtcattgg atgcaaagta
     9601 atttttaatt ctttttcaat ttcttctaaa aggtcaaacc tgtttttacc atctctatcc
     9661 attttattta taaaaacaat tacaggggta tctctcattc tacatacttc cattagtctt
     9721 cttgtttggt cttctacacc attcacgcta tcaataacaa gtattacact atctactgct
     9781 gttaatgttc tataagtatc ttctgcaaag tctttgtgac caggggt
//
LOCUS       DAOQHP010000037         9625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3604_length_9625_cov_4.039080, whole genome shotgun sequence.
ACCESSION   DAOQHP010000037 DAOQHP010000000
VERSION     DAOQHP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_9625_cov_4.039080"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1581
                     /locus_tag="PLY81_02290"
     CDS             199..1581
                     /locus_tag="PLY81_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29089.1"
                     /translation="MILKIKTLLFCLAFTIFCVHAQKKDSTKVSFIDSVKAIKEKTKV
                     TDTSKTSETDSTNNKVDSFYSTTSSAKVVKKLEDPRLGLTYEFITRIKRMKPNQILII
                     DEKFVRNEKRVDEYGLEYEATVTIRFSFSQDMVTGIVKEVPLYATNGRETNLIDIKIP
                     IKFCCTTPMDSVHKKKHCGKMSELNDFEDNDGCKDWVQVEAGSGSKKSSGKPTIKGKG
                     ITGQTSVTGDSNDDDEEEVDKFGKPISKKKKKGKKNKKNKGKIEDEDIEQADSTQSIS
                     KDSLQVSKPSGKKIKGKNKEEEKEGFGKPKQNKKKKGKGRKEEEEDSITENGTTDSTE
                     NKPTNTENKETPSVKKDSTTNEKIETEKKVEEKTESKKDKKKKKKKDNHVEKEEPKID
                     SSNNKTTEPAKEQKTEPAKEVKAETEKKEDKAEQKKEEDKTETKKDKKKKKKKDSKKN
                     NEEEENKNGD"
     gene            1681..2721
                     /gene="dusB"
                     /locus_tag="PLY81_02295"
     CDS             1681..2721
                     /gene="dusB"
                     /locus_tag="PLY81_02295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNF29090.1"
                     /translation="MVKIGDNINLNDFPLLLAPMEDVSDPPFRAICKDNGADLMYTEF
                     ISSEGLIRHAIKSKRKLDIFDYERPIGIQIFGGDEEALALSAKIVDTVNPDLVDINFG
                     CPVKKVVTKGAGAGVLKDVDLMVKLTQAVVKSTKLPVTVKTRLGWNDDTKNIEEVAEK
                     LQDVGIKALAIHGRTRCQMYKGEADWTLIGKVKNNPRINIPIFGNGDIDSPQKAKQYK
                     ERYGVDGIMIGRAAIGYPWIFREIKYFLQTGQELSPPTLLERINVCKKHLLKSVEWKG
                     NKVGIFEMRRHYTNYLKGLPNIKEFRGKLVTMIEVEEIIQTLEEIYNYYNGFEIEKTP
                     IELINYHEKCSL"
     gene            complement(2804..3544)
                     /locus_tag="PLY81_02300"
     CDS             complement(2804..3544)
                     /locus_tag="PLY81_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF29091.1"
                     /translation="MKKIFLLFFITIGFTSYVCSQQVEKASKKKYDLTNRTSDHFMLQ
                     YGMDRWSGAPDSLNVKGSSRHFNFYFMLDKPFKNSPKFSVAYGVGIGSSNIFLDKRYA
                     DIKSNTTVLPFKVSYPGTDSSYFNKFKMTTIFLEAPVELRFYSDPENTNKSFKAALGV
                     KVGTLLKAYSKGKDLKNKSGQSIFGNRFIEKQYDKHFFNGTRLAFTARIGYGIFGLHF
                     SYQITQAIKEGAGAEVKPYSIGLTIGGL"
     gene            3729..4130
                     /locus_tag="PLY81_02305"
     CDS             3729..4130
                     /locus_tag="PLY81_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF29092.1"
                     /translation="MTNELLIGKNILIVEDNAINQMLVKYAFAKSGANTIVAENGSQA
                     LELMNQNKFDAILMDIYMPELDGYQTTDIIRNQLHSSIPIIAMTALAMKGEEEKCLAL
                     GMNGYISKPFSSETLFQEIKRVLEFTEAIAS"
     gene            4249..5769
                     /locus_tag="PLY81_02310"
     CDS             4249..5769
                     /locus_tag="PLY81_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNF29093.1"
                     /translation="MKIVLTTLCYFIVAATFAQTNYNVEKFANTITQKDLKQHLTYIA
                     GNETEGRETATVGQRKAALYIEEQFKNLGLKPGNANSFYQYFKVYQDVLHSSSLKIYD
                     AHFSLNNHYTIQPNSTSNGSISINQIVFASYGLTDKTMNNYENIDIKGKWVMIIESNP
                     EDIDKEKSGADSRKSFTKIKTALDKGAIGVLLVSKNFDNPKYSTLPITGSMYVKKQSN
                     NLSTPIISISYQAAASILTKTIVNFSDLKNIETGTYTTQIVLNNNKETKTLESSNVVG
                     FLEGTDKKEEFVVLTAHYDHLGKKGDVIYYGADDDGSGTVTIIEMAEAFIKAKKKGYA
                     PRRSIVFMTVSGEEKGLWGSDYYTQHPIYPLAKTTVNLNIDMIGRIDPTYKGDSLNYV
                     YIIGDDKLSSDLTPISNNVNKLYKLELDRKYNDPKDPNRFYYRSDHYNFAKNGVPVIF
                     YFNGVHKDYHRPSDTVDKINFDLMTKRARYIFATAWEIANRNDMLKRDIPLNMPAR"
     gene            5828..6442
                     /locus_tag="PLY81_02315"
     CDS             5828..6442
                     /locus_tag="PLY81_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNF29094.1"
                     /translation="MGNIKNIIFDLGSVILNIDYLLTEKAFIQLGITNFSAFYNQHKS
                     SLLFEQLETGNISPEEFYNTFRKITKNSLSNLQIQTAWNAMLLNLPKERINWLLEIKT
                     KYNIFLYSNTNQIHYNCFIDLANNATPSKNFDELFIKAYYSHICGFRKPYKESYLKIV
                     EEQNLQPKETLFIDDTLVNIEGAKLAGLQTLHLLYPKTIIDLSL"
     gene            complement(6501..6707)
                     /locus_tag="PLY81_02320"
     CDS             complement(6501..6707)
                     /locus_tag="PLY81_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29095.1"
                     /translation="MDKSKMIKAYCLKTKEKNVPMHDAVISKTSRGGYMASGNDGKGN
                     KMSAMLGEAKALQAIADGVAKKGF"
     gene            6922..7003
                     /locus_tag="PLY81_02325"
     tRNA            6922..7003
                     /locus_tag="PLY81_02325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6956..6958,aa:Leu,seq:tag)
     gene            7100..8467
                     /gene="tig"
                     /locus_tag="PLY81_02330"
     CDS             7100..8467
                     /gene="tig"
                     /locus_tag="PLY81_02330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNF29096.1"
                     /translation="MATVTRENIGNLTDKLTVNLSKEDYLKNFETSLKKYAKTANIPG
                     FRKGMVPAGLVKKMYGQSVFTDEVLRTVETQLNEYLTKEKLEIFAQPLPLDSDARALD
                     MNNPMDYAFAFEIGLKPSYTINTNFNVTRYKINVTNEMLNDEIDRLQRRFGKMTEPAT
                     VTDDETMLNVTFTEVDKEGNMVENGINKPNSVLVKYFAPAFRANLLGKKLEDVIDVHL
                     ATAFEDKELDVILSDLGLDKNIAGVADKNFKLTITKIGLVEKAAINNELFIAAYPNKE
                     IKTEEEFNNAVKEDIEAYFTQQSNNQIHDQIYHQLLDNTSIDFPEAFLKRWIQTNSET
                     PKTIEEAEQEFPSFKNSLKWTLITSQLSEENKIEVLPEDLRNFAKQQLFQYMGNQLGI
                     LDEQQQWIEDYADKMMKDKKFVEDSYHRISTEKLFNILAQKVNATEESISAEDFASKL
                     HHHHH"
     gene            8626..9561
                     /locus_tag="PLY81_02335"
     CDS             8626..9561
                     /locus_tag="PLY81_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNF29097.1"
                     /translation="MPLAKIMWVDDEMESLQSQKIFLENKGYEVITFSNGFDAIEYVK
                     ENITDVVLLDESMPGITGLETLSKIKEINQQLPVVMITKNETETLMDEAIGSQIADYL
                     LKPVNPNQVLLSLKKIIDNKRLVAEKTTTNYQQQFRELFMALNNNPNYNEWMELYKKL
                     VYWELEMEKSDSPEMQEVLATQKQEANTEFFKFISKNYSKWMHPKTTDAPIMSHNLFQ
                     FKVLPHVEKGTATFFILIDNLRYDQWKAIQPIFTENFRILEEETFYSILPTATQYSRN
                     AIFSGLLPIDIEKKFPLEWKNDDEEGGKNLFEEKW"
BASE COUNT         3921 a         1369 c         1489 g         2846 t
ORIGIN      
        1 tgtagtatta cattgtgaaa gtagatgtgt agaacaataa gttgagcaga ttatcagtaa
       61 taaaatatat ctaaaaatca ttcttctgtt gttgaaataa acagcaaatt aaatagttta
      121 ataacaacca tgatgattat tttattttat tgtatttttc cattttaaaa taacttcaat
      181 ttttacatac ctatttacat gatattaaaa attaaaactc tacttttttg tttagctttc
      241 acaatatttt gtgttcatgc acaaaaaaaa gactcaacca aagtgtcctt tattgatagc
      301 gtaaaagcaa taaaagaaaa aactaaagta acagacacta gcaaaacttc cgaaactgat
      361 tctacaaata ataaggtgga tagcttttat tctacaacaa gttctgcaaa agtggtaaaa
      421 aaattagaag acccaaggct tggtttaacc tatgaattta ttactagaat aaaacgaatg
      481 aagccaaatc aaattttgat aatagatgaa aaatttgtta gaaatgaaaa aagagttgat
      541 gaatatggtt tagagtatga agcaactgta acaatacgtt ttagttttag tcaggatatg
      601 gttacaggta ttgtaaaaga agtgcccttg tatgcaacaa atggtagaga aaccaattta
      661 attgatatta aaatacccat aaaattttgt tgtaccacac caatggatag tgtgcataaa
      721 aagaagcact gtggcaaaat gagtgagcta aatgattttg aggacaatga tggttgtaaa
      781 gattgggtac aggtagaagc aggcagtggt tcaaaaaaat catcaggcaa accaacaatt
      841 aaaggtaaag gcattacagg gcaaacaagt gttacaggag atagtaacga tgatgacgaa
      901 gaagaagtgg ataaatttgg taaaccaatt tctaaaaaga aaaagaaagg caagaaaaac
      961 aaaaagaata aagggaaaat agaggatgaa gatattgaac aagcagatag tacacaatct
     1021 atttcaaaag attcattaca agtttctaaa ccttcaggta aaaaaataaa aggaaaaaat
     1081 aaagaagaag aaaaggaagg ctttggtaaa ccaaaacaaa acaaaaaaaa gaaaggtaaa
     1141 ggcagaaaag aggaagaaga agattctatt acagaaaatg gaacaaccga ttcaacagaa
     1201 aataagccaa caaatacaga gaataaagaa acaccttctg ttaaaaaaga ctccacaact
     1261 aacgaaaaaa tagaaacaga aaagaaagta gaagaaaaaa cagaatcaaa aaaagacaaa
     1321 aagaaaaaaa agaaaaaaga taaccatgta gagaaagagg aacccaaaat tgactcttct
     1381 aataataaaa ctaccgagcc tgcaaaagaa caaaaaacag aacctgcaaa agaggtaaaa
     1441 gcagaaacag aaaagaaaga agataaagca gagcaaaaga aagaagaaga taaaacagag
     1501 accaaaaaag acaaaaagaa aaaaaagaaa aaagatagca agaaaaacaa tgaagaagag
     1561 gaaaataaaa atggagatta ggtaactttt ttatagcact agtaaattat aggttaaaga
     1621 aaatttcaat tgattatttg tagctaattt tgcccctgct tttaaaaaaa taaaatagta
     1681 atggtaaaaa ttggagataa tattaatctg aacgacttcc ctcttctact tgcacctatg
     1741 gaagatgtaa gtgatccccc atttagagcc atttgtaaag ataatggtgc agacttgatg
     1801 tacactgaat ttattagcag tgaaggttta ataaggcatg caataaagag taaaagaaaa
     1861 ttagacattt ttgattatga acgcccaatt ggtatccaaa tttttggagg agatgaagaa
     1921 gcattagcat taagtgccaa aattgtagat acagttaatc cagatttggt ggatattaat
     1981 tttggatgcc cggtaaaaaa agttgttaca aaaggagctg gtgctggcgt attaaaagat
     2041 gttgatttga tggttaaact tacacaggct gttgtaaaaa gcaccaaact acccgtaact
     2101 gtaaaaacaa gattaggctg gaacgacgat acaaaaaaca ttgaagaagt agccgaaaaa
     2161 ttacaagatg ttggtataaa agccttggct atacatggca gaactaggtg tcaaatgtat
     2221 aaaggcgagg cagattggac tttgatagga aaggtaaaaa ataatccaag aatcaatatt
     2281 cccatttttg gtaatggcga tattgatagc ccacaaaagg ctaaacaata taaagaaaga
     2341 tatggtgtag atggtataat gattggcaga gctgctattg gctacccttg gatttttaga
     2401 gaaataaaat attttttaca aacaggccaa gagctttcac ctcctacact tttagaaaga
     2461 ataaatgttt gcaaaaaaca tcttttaaaa tcggttgaat ggaaaggaaa taaagttggc
     2521 atttttgaaa tgcgaagaca ttataccaac tatttaaaag gcttaccaaa tattaaagag
     2581 tttagaggta aacttgtaac catgatagaa gtagaagaaa ttatacaaac tttagaggag
     2641 atatacaact actacaatgg atttgaaatt gaaaaaacgc caatagagtt gataaactat
     2701 cacgaaaaat gttctttgta agagggcaaa ccccaaaaaa attctactaa aaaaaggcag
     2761 tattttttaa aataccacct ttaaaataat ttaaatttaa taattacaat ccgccaatag
     2821 taagtccaat gctataaggc ttaacttcag cacctgcccc ttctttaata gcttgagtta
     2881 tttggtaact aaaatgtaag ccaaaaattc cgtaaccaat tcttgctgta aaagcaagcc
     2941 ttgttccatt aaaaaaatgt ttatcgtact gtttttctat aaacctattt ccaaaaatag
     3001 attggccaga tttattcttt aaatccttgc cttttgaata tgcctttaat aaagtaccaa
     3061 cttttacccc caaagcagct ttaaaagatt tattggtatt ttctgggtct gaataaaatc
     3121 ttaattcaac aggtgcttct aaaaaaattg tagtcatctt aaatttatta aagtaagatg
     3181 aatctgtacc tgggtagcta actttaaaag gtaatactgt tgtattagat tttatatcgg
     3241 catacctctt atctaaaaaa atattactac tgcctatccc aacaccataa gctacactaa
     3301 atttaggact gtttttaaat ggcttatcaa gcataaaata aaaattaaaa tgcctgctgc
     3361 ttccttttac attcaaacta tctggtgcac cagaccatct atccattcca tattgtaaca
     3421 taaaatgatc ggaagtgcga ttagttaaat catatttttt cttcgaagct ttttctactt
     3481 gttggctgca tacataactt gtaaacccaa ttgtaataaa aaataacaaa aatattttct
     3541 tcataaactt taattaaata ggctttaaaa agcactaaag cagcaaaaat aatagaaatg
     3601 gtatcacttt attggttaaa tatttataaa aaattaaatt attttaccct tttgggataa
     3661 tgaattaact tttttatcgt tataacaata taaatccgat tttctttgta aaccaatgct
     3721 tttgaattat gaccaatgaa ttactaatag gaaaaaatat attgattgta gaggataatg
     3781 caattaatca aatgttggta aaatatgctt ttgcaaaatc tggtgcaaac accatcgttg
     3841 cagaaaacgg cagccaagca ttagaactaa tgaaccaaaa taaatttgat gctattttaa
     3901 tggatattta tatgccagaa ttagatggct atcaaacaac agatattatt cgcaatcaac
     3961 ttcattcttc tataccaatt atagcaatga cagccttagc aatgaaaggc gaagaagaaa
     4021 aatgcttagc acttggcatg aatggctata tttctaaacc attttcttcc gaaacattat
     4081 ttcaggaaat aaaaagagtt ttagaattta cagaagctat tgcttcctaa cttaactacc
     4141 ttaataagtt aaaactactc atatctttac cattcacaat tttcagttgt ataataatct
     4201 gtaaaacact attttgcctt ttattattca gaccttaaat aaactacaat gaaaattgtt
     4261 ttaactacac tttgctattt tattgttgct gcaacatttg cacaaaccaa ctacaatgtg
     4321 gaaaaatttg caaacacaat tactcaaaaa gatttgaaac aacaccttac ttacattgct
     4381 ggcaatgaaa ctgaaggaag agaaacagca acagttgggc aaagaaaagc tgcattatat
     4441 attgaagagc aatttaaaaa tttaggctta aagcctggta atgctaattc attctaccaa
     4501 tactttaaag tatatcaaga tgtattacac agttcttcac taaaaatata cgatgcacat
     4561 tttagtttaa ataatcatta taccattcaa ccaaatagta caagcaatgg tagcatttca
     4621 attaatcaaa ttgtttttgc aagctatggc cttactgata aaacaatgaa caattatgaa
     4681 aatattgata taaaaggaaa atgggtaatg ataatagaaa gtaaccccga agatattgac
     4741 aaagaaaaat ctggagcaga ttccagaaaa agttttacaa aaattaaaac cgctttagac
     4801 aaaggtgcta taggtgtttt attagtttct aaaaactttg ataatccaaa atatagtaca
     4861 ctaccaatta ctggtagtat gtatgtaaaa aaacaaagca ataatctttc tacacctatt
     4921 atctcaattt cgtatcaggc agcagcatct attctaacaa aaaccatagt taattttagc
     4981 gatttaaaaa atattgaaac aggtacttat actacacaaa tagttttaaa taataataaa
     5041 gaaacaaaaa cacttgaaag tagcaatgta gttggctttt tagaaggaac agataaaaaa
     5101 gaagagtttg ttgtattaac tgcacattac gaccacttag gtaaaaaagg agatgttatt
     5161 tattatggag ccgatgatga cggaagtgga acagttacta ttattgaaat ggcagaagct
     5221 tttattaagg caaagaaaaa aggatatgca ccaagaagaa gtattgtttt tatgactgta
     5281 agcggagaag aaaaaggttt atggggcagc gattattaca cacaacaccc aatttatcct
     5341 ttagccaaaa ctactgtaaa cctaaatatt gatatgatag gtagaataga cccaacctat
     5401 aaaggcgata gcttaaatta tgtttatatt attggagatg ataagttaag tagcgattta
     5461 acacctatta gcaataatgt aaacaaattg tataaattag aattagatag aaaatacaat
     5521 gaccccaaag atcctaacag attttattac agaagcgacc attacaattt tgcaaaaaat
     5581 ggagtacctg ttatttttta ttttaatgga gtacataaag attaccacag accaagtgat
     5641 acagttgata aaataaattt tgatctaatg actaaaagag ccagatacat ttttgccact
     5701 gcatgggaaa tagctaatag aaatgatatg ctaaaaagag atattccttt aaatatgcca
     5761 gcaagataat taatttaaaa tacagataaa agagttatta atattgcaac tattaataac
     5821 tttttttatg ggtaatatca aaaatatcat ttttgatttg ggtagtgtaa ttttaaacat
     5881 agattattta ttaaccgaaa aagcttttat acaacttggt ataactaatt tttcagcgtt
     5941 ttataatcaa cataaatctt ctttgttatt tgaacaatta gaaacaggaa atattagccc
     6001 cgaagaattt tataatacat tcagaaaaat taccaaaaac tctttatcta atcttcaaat
     6061 acaaactgca tggaatgcca tgttgcttaa tttaccaaaa gaaagaatta attggctact
     6121 agaaataaaa accaagtaca atattttttt atactccaat accaaccaaa ttcattataa
     6181 ctgctttata gatttagcca ataacgccac tccatctaaa aattttgatg agctatttat
     6241 taaagcatat tactctcata tttgtggttt tagaaaacct tataaagaaa gctatttaaa
     6301 aatagtagaa gaacaaaacc tgcaacctaa ggaaacactt tttatagatg acactttagt
     6361 taatatagaa ggagcaaaac ttgcaggctt acaaacctta catcttttat accccaaaac
     6421 aattatagac ctatctctat aaaaaataca gtattaaagt tgttcacttt aatactgtaa
     6481 cataaaaaaa ttaaaagcta ttaaaaacct tttttagcaa caccatctgc aattgcctgt
     6541 aaagcttttg cttcgcctag cattgccgac attttattgc ctttaccatc attgccagat
     6601 gccatgtaac cacctcttga tgttttactt ataacagcat catgcattgg tacatttttt
     6661 tcttttgttt taaggcaata ggctttaatc attttgcttt tgtccataat tattcatttt
     6721 tgggttagat aacttattag cgatgcacaa actacagttt ttctgataaa tacaataaaa
     6781 tattaccttt tttatattca caatatgttg gtattacttt tttagcaaaa tagtaaccat
     6841 aaaaaacttt taaactaaat gatacaattg tgaaatttta caataaaaac cctacttttg
     6901 ccaccctaaa tttatacacc tgcgggtgtg gtgaaattgg tagacacgcc agacttagga
     6961 tctggtgccg tgaggcatgg gggttcgagt ccctccaccc gcactaatgt gaagttgata
     7021 agttgagggg ttgaacaatt ctcttcaact cttttcttat caacttcttt attttaaaaa
     7081 catacaacag gcccttacta tggcaacagt aaccagagaa aatattggta accttaccga
     7141 taaacttaca gtaaatctta gcaaagaaga ttatttaaaa aactttgaaa caagcttaaa
     7201 gaaatatgct aaaacagcta atatacctgg ctttagaaaa ggtatggtac cagcaggttt
     7261 agttaaaaaa atgtatggcc aaagcgtatt taccgatgaa gttttgcgta ctgtagaaac
     7321 acagttaaac gaatatctta caaaagagaa attagaaatt tttgcacagc ctttacccct
     7381 agatagcgat gctagagcat tagatatgaa taatcctatg gattatgctt ttgcttttga
     7441 aatagggcta aaaccaagct ataccattaa tactaatttt aatgtaacca ggtataaaat
     7501 aaatgtaacc aacgaaatgt taaacgatga aatagatcgt ttacaaagaa gatttggtaa
     7561 aatgacagag ccagcaacag ttactgatga cgaaactatg ttaaatgtaa cgtttacaga
     7621 agtagataaa gaaggtaaca tggtagaaaa tggtattaat aaaccgaata gtgttttagt
     7681 taaatatttt gctcctgctt ttcgtgccaa tttattaggt aaaaaattag aagatgttat
     7741 tgatgttcat ttagcaacag cttttgaaga taaagaatta gatgtaatat taagcgattt
     7801 aggattagat aaaaatattg ctggagttgc agataaaaac tttaaactta caattactaa
     7861 aataggatta gttgaaaaag cagcaatcaa taatgaactt tttatagctg catacccgaa
     7921 caaggaaata aaaacagaag aagaatttaa taatgctgta aaagaagata tagaagccta
     7981 ctttactcaa caaagcaaca atcaaattca cgaccaaatt tatcatcaat tattagataa
     8041 tacttctata gattttcctg aagcattttt aaaacgttgg attcaaacaa acagcgaaac
     8101 tccaaaaaca atcgaagaag ctgaacaaga atttccttct tttaaaaata gtttaaaatg
     8161 gacgctaatt acctctcaac tatctgaaga gaataaaatt gaagtactac cagaagattt
     8221 aagaaatttt gctaagcagc aattatttca atatatgggt aatcaattag gtattttaga
     8281 tgaacaacaa caatggatag aagattatgc agataaaatg atgaaagata aaaaatttgt
     8341 agaagatagt taccacagaa taagtactga aaagttattt aatattttag cacaaaaagt
     8401 aaatgcaaca gaagaaagta taagtgccga agattttgct agcaagttgc accaccatca
     8461 tcactaattt gtttattaat aataatttta aatctctgca ataattaaaa aatgcagaga
     8521 ttttttgttt cagctcccct ctacattttt ataaaagatt tatataaaaa caactttaac
     8581 aacaatattt aatagttact tttatcttcc gtaaaaataa acaatatgcc attagctaaa
     8641 attatgtggg tagatgatga aatggaaagc ctacaaagtc aaaaaatatt tttagaaaac
     8701 aaaggctacg aggtaattac ttttagtaat gggtttgatg ctatagaata tgtaaaagaa
     8761 aacattaccg atgttgtttt gttagatgaa agcatgccag gcattacagg ccttgaaacc
     8821 ttatctaaaa taaaagaaat aaatcagcaa ttaccagtag tgatgataac taaaaatgaa
     8881 acagaaacac taatggatga agccataggt agccaaattg cagattattt gctaaaacct
     8941 gtaaatccta atcaggtttt attgagttta aaaaaaataa ttgataacaa aagattagtt
     9001 gctgaaaaaa ctacaaccaa ctaccaacaa cagtttagag aattgtttat ggccttaaac
     9061 aataatccaa attataacga atggatggaa ctttataaaa aattagttta ttgggagctt
     9121 gaaatggaga aaagtgatag ccccgaaatg caagaagtat tagctacaca aaaacaagaa
     9181 gccaatacgg aattttttaa atttatcagt aaaaattatt ccaaatggat gcatcccaaa
     9241 acaaccgatg cacccattat gagccataat ttatttcaat ttaaagtttt gccacatgtt
     9301 gaaaaaggta ctgctacatt ttttatttta atagataatt tacgatacga ccaatggaaa
     9361 gctattcagc caatttttac agaaaacttt agaattttag aagaagaaac cttttattct
     9421 attttaccaa ctgctacaca atacagtaga aatgctatct tcagcggatt actgccaata
     9481 gatatagaaa aaaaatttcc gttagaatgg aaaaacgacg atgaagaagg aggtaaaaat
     9541 ttatttgaag aaaaatggta aacttaaaaa taaaatatag gaggaagtta aatatgtcac
     9601 atgcagttat gattccaaga aaagt
//
LOCUS       DAOQHP010000038         9456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3687_length_9456_cov_3.576747, whole genome shotgun sequence.
ACCESSION   DAOQHP010000038 DAOQHP010000000
VERSION     DAOQHP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3687_length_9456_cov_3.576747"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLY81_02340"
     CDS             complement(<1..392)
                     /locus_tag="PLY81_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29098.1"
                     /translation="MKKLIVIFTVVLSAAVIAFVGCQKTSTEKQEQGIVGTTEVSANL
                     LGMSVNSSYGFLVFTTTDDVDAYLDYLKANTHADVQEYLSSIGFSNSLGATLYDEEYA
                     TELVTEEQAINYIVNTHRIFQVQDVIMKP"
     gene            677..1030
                     /locus_tag="PLY81_02345"
     CDS             677..1030
                     /locus_tag="PLY81_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF29099.1"
                     /translation="MYQTGNKIKKLRELRNFTQGYMAEKLGISQSNYARLENDEVGIS
                     NERLKQIADVLETTKENILSFDEKYVFNVHNEQHSSGAIVHNYSKSPEIKQLYEDKIK
                     LLEEKVQMLEKQLSS"
     gene            1191..1664
                     /gene="rpsP"
                     /locus_tag="PLY81_02350"
     CDS             1191..1664
                     /gene="rpsP"
                     /locus_tag="PLY81_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNF29100.1"
                     /translation="MAVKMRLQRHGSKKRPFYFIVVADARAPRDGKFIQKIGTYNPLT
                     VPASIQLDRQKALDWLHKGAQPTDTVRRILSFKGVLYLKHLLRGVKLGLFDDATAMTK
                     FQAWHEEHEAHVKRRNDSHRNTQRQKKAYVPPVRKVEEVAQPANDAPAAEENTEA"
     gene            complement(1722..2444)
                     /locus_tag="PLY81_02355"
     CDS             complement(1722..2444)
                     /locus_tag="PLY81_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29101.1"
                     /translation="MKYFINKKLLTLTVAFFFFTLAAIFIQLFAFQPVAKYFSYKTSI
                     SYDATGLCKSHPKDSFIERLYEQYAYEIENNLEFSGKFQLQKYERQQLIELILIDGAA
                     PLIFIILGIVGFVYLIKYKKNINYNNLSLIQWIAVFLNLFWFRWLLLLVLYIVFVSLS
                     KKDNALLIINETNIAYALNISPLFILIPLGLFSTWICYITVFVFIPKVYRGKFLLTGV
                     IGCILALLFWFKLIGAMVLPYE"
     gene            complement(2454..3470)
                     /locus_tag="PLY81_02360"
     CDS             complement(2454..3470)
                     /locus_tag="PLY81_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29102.1"
                     /translation="MNNRSFSNEGIFFSDILMPSESFLEIFDFLKSNISGLDALSTSN
                     LFRINIFLSQGDSLSNLQISNIKGFSIFCKNENSFSHHLFRVDNGVVIQGEQEVLCGG
                     EFQECSISDLRGIGYYLNDEISPSAIINLMNDIDIEFETDITKQYLSKLLYKTQYITP
                     EIYVEGDDFGPNCSHCGERNRKVSCKWTGKERECPSCIKDILSKTNVDLELGYGELSS
                     EDAHSFRDNFMAESNVGRKYIIYYYVLSDFALAEMPLTINSISDYYEFAIDCYAIKNI
                     IENGSNNKVVVNSNFKEKALAKIEYFKGVTTNIKILTILDDIKLDIINWHGLSKGALL
                     EALN"
     gene            3965..4885
                     /locus_tag="PLY81_02365"
     CDS             3965..4885
                     /locus_tag="PLY81_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF29103.1"
                     /translation="MKQVLEILKLGQKNFSIPGMDLLYEKEQNIPGSINYNISRYYAQ
                     DAWLAEDTGMMVYHFNENKPEENFLELRFCASGNKYCFDKSCSFCEKQPTDCNGQQTT
                     LDVFSFHFSSTFLYQFTQNVKVTNRKDEVLAFKHPNAFTKVFPVCPKKRNVLDSLLNH
                     SYTGALENIFINAKIHELLLYSLDCLVDEKEEGFNCKFLEDERSRLAIYDARDILLKH
                     IGDPLTIKELSRKVAINECYLKKGFKEIFGTTIFDFYQQQRMEHAKYLLYEKSLSVTD
                     ASALLGYSSISHFSAAFKKHTGLKPCELLK"
     gene            complement(4888..8127)
                     /locus_tag="PLY81_02370"
     CDS             complement(4888..8127)
                     /locus_tag="PLY81_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF29104.1"
                     /translation="MKKTISLIILISCFITKINAQDLETELNSNISMYKKYVSEKNYT
                     AAIPYIKKSALLVKQKYGEQNKDYLTLINSTGTLYFFISKFDTAVLYYDTTLTLYKKY
                     YSADKEGLLQTHLNLAMSYNKMEDYTNAKKWYEIVVASQKELYGTNSKNYRMSYGNLA
                     NVYKNLGLYEEAEKMMLENIDYYEKMDSKSEAYEIALQNLAALYQKWGLHYWAAMYYF
                     IASDVRLVIAGENDTYFKNYISNATFYFEKAAGECRKDGDFERAEKCYNYNTIIFKER
                     KNNKEYARFLNLRGLMELELKKYTNAEISFFTALQIFKKEFDTTHLSVGMVYNNIGML
                     YFNLNRYDFAEDYCYEGYKIRLKRLNKNDKDIAESCCNLGLIYTYTMQYDKAEEFNLK
                     AKQIYENGIGTNSSEYAIALNNLGMLYWKKKNYEEAEKYYLQSLQLREKLYGKEHTSY
                     ARILNNLGTIYLEWENPTKAIKYFEQAVAISEKKFGKNHIRNIPYLNNLALCYNDLGN
                     YNEAKKIIINTNQLNSKLIKDNFTILTEYEKELYADEILYNSKYANSILYNSRNYNSE
                     NIIDNLNQQLQLKGLVLNETKTFYNYVKNNSDTAFQKMFNEYSQLKTRIAREYGFAIE
                     NRSKDLSSWEGRVDVLEKDLLLKSKKFTEQQKGIEVNYNTLKNSLKEDEMLIEFAKFN
                     LYNKGETDSVLYVAYIVTKNGNAPVYVPLFEESELQKILTSAGKTNQALASNLYRSTI
                     TKNKSKANFGNELYNLVWQPLEPYLKNKKKIYFAPVNKLNTIAFNALAIDSNTYLIDK
                     YQLFQYTSTRMFVQDKKEVNKVANIALFGNADFSLTQSTIKTYQQNADSAIVKRGFNK
                     ENTTLSTWATLPGTAEEIKNIGTLFSKKSIPQYVYTNTNASEENLKKLNDQNISIIHL
                     ATHGFFLPKIKETQQSISNSTTTNYNTAYDPMLRSGIILSGANYAWNGGFVPKEKEDG
                     IVTAYEISQLDLSNTNLVVLSACETALGDIQGNEGVYGLQRAFKLAGVKNIILSLWQV
                     PDKETAELMTIFYNNYLSGKSIDESFNLTQQSLRKKYSPYYWAAFILIK"
     gene            8199..>9456
                     /locus_tag="PLY81_02375"
     CDS             8199..>9456
                     /locus_tag="PLY81_02375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF29105.1"
                     /translation="MIKLIFFILALTFIGCHTKENADLIVLNAKIYTVDSAFSLAEAM
                     AIKDGKILALGKAESILDSYVAKEKFDANGKFIYPGFIDAHAHFVGYGKSLFEVQLYD
                     SKSWNEVIGRVTRFAKNNPNEKWIIGRGWDQNKFEGKQFPTNELLNQLFPDVPVMVTR
                     VDGHAAIANAKALALAEIKPNQQLTGGSIETKNGKLTGLLIDNAVDLVQSKIPGAALK
                     DYDKWLMAAQENCFEQGLTTITDCGLMYNDVEKIDSLQQQNKLLMKLYVMLSDDQRNY
                     EKYLSKPPYKTNKLFVKGIKVYSDGALGSRGACLLQHYSDKPNWFGFLLSNKAHFDSL
                     ASILVNTPYQMCTHAIGDSANRTILTIYNKYLKGKNDKRWRIEHAQVINENDFDLFGK
                     ASVIPSVQPTHATSDMYWAEERLGKKR"
BASE COUNT         3268 a         1423 c         1338 g         3427 t
ORIGIN      
        1 ggtttcatta tcacatcctg tacttgaaat atcctgtgtg tatttacaat ataattaata
       61 gcttgctcct ccgttacaag ttcagtggca tattcttcat cgtacaaggt agcacccaaa
      121 ctattagaaa atcctattga gctaaggtat tcctgcacat cggcatgggt atttgctttt
      181 aaataatcaa ggtatgcatc tacatcatct gttgtagtaa ataccaaaaa accgtaacta
      241 ctatttacac tcatacctaa taagtttgca cttacttctg ttgtaccaac tattccttgt
      301 tcttgttttt cagtactcgt tttttggcat ccaacaaagg caattacagc agcacttagc
      361 acaactgtaa aaataacaat taattttttc atggtttata attttttaaa attttaagta
      421 atattttttg aaaatcagag ctattgtttg tagtctttgg aggagattta ttgggttgta
      481 acataaaaat atagtttagg gtttttaaaa aactgaacaa caaaacaggt attccgctaa
      541 aaaaatttaa ttaataacta aatgttgttg ttggctttat ctgttttgcc aatgctatat
      601 tgcttatttt tctttacatt tttaattgca taaatgggta gtttatgtat tttcgtacat
      661 aaattatcca aacaatatgt accaaacagg caataaaata aaaaagctga gagagctgcg
      721 aaactttaca caaggctata tggcagagaa attaggtatc agtcaaagta attatgcccg
      781 tttagaaaat gatgaagtag gcataagcaa cgaacgtctt aaacaaattg ctgacgtttt
      841 agaaactact aaagagaata tattgagttt tgatgagaaa tatgtgttca acgttcataa
      901 tgaacaacac tcaagtggag ctattgttca taactattct aaaagtcctg aaataaaaca
      961 actatacgag gacaaaataa agcttttgga agaaaaagta caaatgttag aaaaacaatt
     1021 aagtagctaa ttgtttaata ataacttacc gaacaaaggt tttacattaa actttgttaa
     1081 gagctaaaaa aatctttggc agccccaaaa aatacttata cattcgctgc ccaatcattc
     1141 atgattgtat tattaaccaa attgttaaag cctttaaatt tctatttatt atggcagtaa
     1201 aaatgagatt acaaagacac ggcagtaaaa aacgcccttt ctattttata gtagtggcag
     1261 atgcacgtgc acccagagat ggtaagttta ttcaaaaaat tggaacttac aatccattaa
     1321 ctgttcctgc aagcattcag ttagatcgtc aaaaagcatt ggattggcta cacaaaggag
     1381 cacaaccaac cgatacggtt cgcaggattc tctctttcaa aggagtgttg tatttaaagc
     1441 atttattgcg tggtgtaaaa ctgggcttgt tcgatgatgc tacagctatg actaagtttc
     1501 aggcatggca tgaagaacat gaagctcatg taaaacgtag aaacgattct catcgtaata
     1561 cacaacgtca aaagaaagct tatgtacctc ctgttcgtaa agtagaagaa gtagcacagc
     1621 ctgcaaatga tgcacctgct gctgaagaaa atacagaagc gtagcaatag caacgtaatt
     1681 ttcccaaaaa tccctgctta aaaatttaag cagggatttt tctattcata aggtaaaacc
     1741 attgctccta taagtttaaa ccagaatagc aacgctaaaa tacagcctat tactcctgtt
     1801 aataaaaatt tgcctcgata tactttggga ataaaaacaa aaacagtaat ataacatatc
     1861 caagtagaaa acaaacccaa tggtatgaga atgaacaatg gagatatatt taatgcgtaa
     1921 gctatattcg tttcatttat tattaaaaga gcattgtctt ttttacttaa actcacaaaa
     1981 actatgtata gtacgagaag taataaccat ctgaaccaaa ataaattaag aaaaacagct
     2041 atccattgta ttaaggacaa gttgttatag ttaatatttt tcttatactt tatcagataa
     2101 acaaaaccca ctatacccaa gattataaat attagtggtg ctgctccatc tatcaatatt
     2161 agttcaataa gttgctgcct ttcgtatttt tgtaattgaa actttcctga aaattcaaga
     2221 ttattttcta tttcataagc atactgttcg tatagtcttt caataaaact atcttttgga
     2281 tggcttttac ataaacctgt tgcatcataa ctaatacttg ttttgtatga aaaatatttt
     2341 gcaacaggtt gaaaggcaaa aagctgaatg aaaatagcag ctaatgtgaa aaagaaaaat
     2401 gcaacagtta aggtaagaag ctttttattt ataaaatatt tcattcaata tttttaattt
     2461 aatgcttcta ataatgctcc tttagatagt ccatgccagt taataatatc cagcttaata
     2521 tcatctaaaa tagtcaaaat tttaatattc gttgtaaccc ccttaaaata ctcaatttta
     2581 gctaaagctt tttctttgaa atttgaatta actacaactt tattattact tccattttca
     2641 ataatatttt tgattgcgta gcaatcaata gcaaactcgt aataatctga gatgctatta
     2701 atggttaatg gcatttcagc taaagcaaaa tctgataaca cataataata tattatatac
     2761 tttctgccca cattagattc tgccataaaa ttatctctaa aagaatgagc atcttctgac
     2821 gataattctc cataaccaag ttctaaatct acattcgttt tagataatat atcttttata
     2881 caactagggc attccctttc cttccctgtc cacttacaac taactttacg attcctctca
     2941 ccacagtggc tacaattagg accgaaatca tcaccttcta catatatttc aggtgtaata
     3001 tattgggttt tgtaaagtaa cttacttaaa tattgcttag ttatgtcagt ttcaaattca
     3061 atatcaatat cattcataag atttattatt gcacttggag atatttcgtc atttaagtaa
     3121 tacccaattc ctcttaaatc agaaatacta cattcttgaa attcacctcc acataaaact
     3181 tcttgttcgc cttgtattac aactccattg tcaactctga ataagtgatg ggaaaaggag
     3241 ttttcgtttt tacagaaaat actaaaccct ttaatattag aaatttgtag attacttaag
     3301 ctatcgcctt gtgataaaaa aatgtttatt ctgaaaagat ttgaagtaga aagagcatct
     3361 aaaccagaaa tattggactt taaaaaatca aatatttcta agaaagattc agaaggcatt
     3421 aaaatatcac taaaaaaaat accctcattt gaaaaactcc gattattcat attatttgta
     3481 aaaaatcaga tacttttctg ctcataaaat tttgaagaat ttactactat atattctctt
     3541 ccaaaaagtt tttctttgaa tatttgcttt tcagtagtat tatatatgtt tttctcctta
     3601 aaacaagaaa caaatataat agtacaaagt actgtaagaa tagcaatagc agtcagcgac
     3661 tttcctaaaa tgggtttctt tttcatattt tatagtttag ttatccaaat gtaagcaatc
     3721 taattaacta gattaactat ctttaacagt tttagagcca cctgtttaaa atttaatcta
     3781 taaatcctgt attgttcaaa aacaaccgat tttaagtacc cttttgtatc tattttagtt
     3841 tttttaaagc cataaaaagc attaaaatga accaatagta cttgaaaatt aaaattttaa
     3901 acaggctcta aaaataaaaa aacttggaat ttacatgtat atacattaat tttgtactct
     3961 tgaaatgaag caagtattag aaatattaaa attaggtcaa aaaaatttta gtatcccagg
     4021 tatggatttg ttgtatgaaa aagaacaaaa cattccgggt tctattaatt ataatattag
     4081 tagatactac gctcaagatg catggcttgc agaagataca ggtatgatgg tttaccactt
     4141 taatgaaaac aaacctgaag aaaatttttt agaattacgt ttttgtgctt ccggaaataa
     4201 atattgtttt gataaaagtt gtagcttttg cgaaaaacaa ccaaccgatt gtaatggaca
     4261 acaaacaacc ttagatgttt tttcttttca cttctcttct acattcttgt atcagtttac
     4321 acaaaatgta aaagtaacca acagaaaaga tgaggttttg gcatttaagc accctaatgc
     4381 ttttacaaaa gtttttcctg tgtgtcctaa aaagagaaat gtgttagatt ctctattaaa
     4441 tcatagttat accggtgctt tagaaaacat atttatcaat gccaaaattc atgaactttt
     4501 actttatagt ttagattgcc tggtagatga aaaagaagaa ggatttaatt gtaagttttt
     4561 agaagatgaa cgtagccgat tggcaattta cgatgcaagg gatatattat taaaacatat
     4621 tggagaccct ttaacaataa aagaattaag ccgaaaagtt gctataaacg aatgttattt
     4681 aaagaaaggg tttaaagaaa tttttggtac taccattttc gatttttatc aacaacaaag
     4741 aatggaacat gctaaatatt tgttgtacga aaaaagttta agtgtaacag atgcatctgc
     4801 attattaggc tattcatcta tatctcattt ttctgcagca tttaaaaaac atactggttt
     4861 aaaaccttgt gaactgctaa aataatttta ttttatcaat ataaatgcag cccaataata
     4921 tgggctatat ttttttctta aactttgttg tgtcaaatta aaagattcat ctattgattt
     4981 tccacttaaa taattgttgt aaaatattgt catcagttca gcagtttctt tatctggtac
     5041 ttgccataaa cttaaaataa tattttttac accagccaat ttaaaagctc tttgtaaacc
     5101 gtacacacct tcatttcctt gaatatctcc taaagctgtt tcgcaagcac taagtacaac
     5161 aagattagta ttacttaaat ctaattgaga aatttcgtaa gcagtaacaa taccatcttc
     5221 tttttcttta ggtacaaatc caccattcca agcatagttt gcaccactta aaataattcc
     5281 acttcgtaac atagggtcgt aagcagtatt gtaatttgta gttgtactgt tagagattga
     5341 ttgttgcgtt tctttaattt taggaagaaa aaaaccatgt gttgcaagat gaataattga
     5401 aatattttgg tcgttcaatt tttttaaatt ttcttccgat gcgttggtat ttgtataaac
     5461 atattgtggt atagattttt tgctgaacaa agtgccaata tttttaattt cttctgcagt
     5521 gccaggtagg gtagcccatg ttgataaagt tgtattttct ttattaaaac ctcttttaac
     5581 aattgctgaa tcggcatttt gttgataagt tttaatagta ctttgcgtaa ggctgaaatc
     5641 tgcatttcca aaaagtgcaa tattggctac tttatttact tcttttttat cttgcacaaa
     5701 cattctggta gatgtgtact gaaacaactg atatttatca attaaataag tattgctatc
     5761 tatagctaaa gcattaaatg caatagtgtt taatttattt actggtgcaa aatatatttt
     5821 ctttttattt tttaaatatg gctctaatgg ttgccaaacc aaattatata attcatttcc
     5881 aaaatttgct ttagatttat tttttgtgat agtacttcta taaagattac ttgccaaagc
     5941 ctgattggtt ttgcctgcac ttgttagtat tttttgtaat tctgattctt caaataaagg
     6001 aacatataca ggagcattac cattttttgt tactatatat gcaacatata aaacagaatc
     6061 tgtttcgcct ttattatata aattaaattt agcaaattca atcaacattt cgtcctcttt
     6121 caaactgttt tttagtgtat tataattaac ctcaatacct ttttgttgtt ccgtaaattt
     6181 tttacttttt aataataaat ctttttctaa aacatctact cttccttccc atgaacttaa
     6241 atctttcgac ctgttttcaa tagcaaatcc atattctctg gctattcttg tttttaactg
     6301 gctatattca ttaaacattt tttgaaaagc tgtatccgaa ttatttttta cataattata
     6361 aaatgttttt gtttcgttta ataccaaacc ttttaattgt aattgttgat ttaagttgtc
     6421 tattatgttt tcgctattat aatttctact attgtataaa atactgtttg catattttga
     6481 attatataaa atttcatctg catacagttc tttttcgtat tcagtaagta tagtaaaatt
     6541 atcttttatt agtttactgt taagttggtt agtattaatt attatttttt ttgcttcatt
     6601 gtaattacct aagtcgttat agcataaagc aagattattt aagtaaggaa tattacgaat
     6661 atggttttta ccaaattttt tttcagaaat agctacagct tgctcaaaat atttaatagc
     6721 ttttgtgggg ttttcccatt caaggtatat agtgcctaaa ttatttaata ttctggcgta
     6781 agaagtgtgt tctttgccat ataatttttc tcttaattgt aagctttgta aatagtattt
     6841 ttctgcttct tcataatttt ttttcttcca gtataacatg cccaaattgt ttaatgcaat
     6901 ggcatattca ctactgtttg tgccaattcc attttcataa atttgcttgg ctttcaaatt
     6961 aaattcttcc gctttatcat attgcattgt gtaggtatat attaaaccta aattgcaaca
     7021 actttcggca atatctttat cgttcttatt aagccttttc aatcttattt tgtagccttc
     7081 ataacaataa tcttcagcaa aatcgtatct gttaaggtta aaatataaca taccaatgtt
     7141 attatatacc atgccaacag ataaatgtgt tgtatcaaac tcttttttaa aaatttgaag
     7201 ggctgtaaaa aaactaattt cggcattggt atattttttt agttctagtt ccattaatcc
     7261 tcttaggttc aaaaatcttg catactcttt attatttttt ctttctttaa aaatgatagt
     7321 attgtaatta tagcattttt cagccctttc aaaatcacca tcttttctac attctcctgc
     7381 tgccttttca aaataaaaag ttgcattaga tatataattt ttaaagtagg tatcattttc
     7441 accagctata acaagcctaa catcagaagc aataaaataa tacattgctg cccaataatg
     7501 caagccccat ttttgataaa gtgctgctaa gttttgtaat gcaatttcat aggcttcgct
     7561 tttagaatcc attttttcat aatagtcaat attttctaac atcatttttt ctgcttcttc
     7621 atacagccct aaattcttat acacattggc taagtttcca taactcattc tataattttt
     7681 actatttgtt ccatacagtt ctttttgaga tgccaccaca atttcatacc attttttagc
     7741 attggtataa tcttccattt tattataact catggctaag ttgaggtgcg tttgtaaaag
     7801 cccttcttta tctgctgaat aatatttttt atataaggtt aatgtagtat cgtaatacaa
     7861 aactgctgta tcaaactttg atataaaaaa atacaaagtg cctgttgaat ttattaaagt
     7921 caaataatct ttattttgtt ctccatactt ttgttttaca agtaatgcag atttttttat
     7981 gtaaggaata gcagcagtat aatttttttc agaaacatat tttttataca tactgatatt
     8041 gctatttaat tcagtttcta aatcttgtgc atttattttt gtaataaagc aacttattaa
     8101 gattattaaa gagatagttt ttttcattga taataaatat ttccgaagtt ataaatttga
     8161 attaataatt tcaataccca ataaacagta tattttatat gattaagtta atttttttca
     8221 ttcttgcatt aacctttata ggttgccata caaaagaaaa tgcagattta attgtattga
     8281 atgctaaaat ttatacagtg gatagtgctt tttctttagc tgaggcaatg gctataaaag
     8341 atggtaaaat acttgcattg ggtaaagcag aaagtatttt agatagttat gttgctaaag
     8401 aaaagtttga tgcaaatgga aaatttattt accctggttt tattgatgca catgcacatt
     8461 ttgtaggtta tggtaaaagc ttgtttgaag tacaattata cgatagcaag tcctggaatg
     8521 aagtgatagg aagggttaca aggtttgcaa aaaataatcc taatgaaaaa tggataatag
     8581 gcagaggttg ggaccaaaat aagtttgagg gtaaacaatt tccaaccaat gaattattaa
     8641 atcaattatt tcctgatgtt cctgttatgg taacaagagt agatggccat gcagccattg
     8701 caaatgcaaa agccttagca cttgcagaaa ttaagccaaa tcaacaatta acaggaggaa
     8761 gtattgaaac taaaaatgga aaacttacag gactattaat tgacaatgca gtagatttag
     8821 tacaatctaa aatacctgga gcagctttaa aagattatga taaatggtta atggctgcac
     8881 aagaaaattg ttttgaacaa ggcttaacta ccattacaga ttgtggctta atgtataacg
     8941 atgtggaaaa aatagattct ttacagcaac aaaataaatt gttgatgaag ctatatgtaa
     9001 tgttaagtga tgatcaaaga aactatgaaa agtatttgag caaaccacct tataaaacaa
     9061 ataagctatt tgtgaaagga attaaagtgt atagcgatgg tgctttaggt agccgtggtg
     9121 cttgtttgct acagcattat tcagataaac caaactggtt tggattttta ttaagcaaca
     9181 aagcacattt tgattctttg gcttctattt tagtgaatac accctatcaa atgtgtacac
     9241 atgccattgg tgatagtgct aaccgtacta ttttaactat ttacaataaa tatttaaaag
     9301 gcaaaaatga taaacgttgg cgtatagaac atgcccaagt tattaatgaa aatgattttg
     9361 atttatttgg caaagcttct gttattcctt ctgtacagcc tacacatgcc acaagcgata
     9421 tgtattgggc agaagaaagg ttagggaaaa aaagac
//
LOCUS       DAOQHP010000039         9291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3760_length_9291_cov_3.930598, whole genome shotgun sequence.
ACCESSION   DAOQHP010000039 DAOQHP010000000
VERSION     DAOQHP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_9291_cov_3.930598"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLY81_02380"
     CDS             <1..699
                     /locus_tag="PLY81_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNF29106.1"
                     /translation="QTIVSLFYPIQLNSLDKGLWLIAATAIINFIAGTICLQIGKKNN
                     SIALKATGKHLYTDTLSTIVVIAGLLIMLYSKMYWLDKVLAFAISFIILYNGYKIIRT
                     SMAGIMDEADMELLKQIIKVLNENRRENWIDLHNFRVIKYGRLLHIDCHLTIPWYLNI
                     HQAHEEVDLLTNLIKEKFGGGIELFVHTDGCLNFSCSICLKQNCIERKRPFLHKIEWT
                     LENVLSNQKHNSVI"
     gene            complement(692..1354)
                     /locus_tag="PLY81_02385"
     CDS             complement(692..1354)
                     /locus_tag="PLY81_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29107.1"
                     /translation="MKKTLIAFIAFIAILSLNSCKKSSSGSTSTDTYLPNNVGTNWKY
                     KVTTGGASSDVTYTVSTNTLVVDGITYKIITGSNGNEQYYAKVGSDYHSLIKVSGQNV
                     HLIILKDDKNVNDTWTTTQTLTGLSIPGVPVSSATVNITYTLKEKGTTRVVEGVTYNN
                     VIKVEAALSASAAGFPLSLGTIEYYFSKNIGIIENTANINNSTIGVSINDKYELKTYE
                     IK"
     gene            1526..3202
                     /locus_tag="PLY81_02390"
     CDS             1526..3202
                     /locus_tag="PLY81_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNF29108.1"
                     /translation="MKLLAASFLVLISFNSIAQQKNSILITSQLSSANVFYGYGAELT
                     HKAKANIKKGQQEIVIKNISTTADENTLQIATPENVVLLSYKLEIKQEGIKPTNPLIK
                     KMEDSLKILQKQINANNQDWQIQDEQLTKTSKILETYTSSTGKNINTLEVLKLIDYYN
                     GKVQFFRNSMYAIKIKVNDLQEQVFDITNRLVELRKKEYAEAKKPIANFVLQVISKEA
                     MEADFAISYYTQNAGWVPTYDMRIKSIDNNFKLAYKALISQTTGIDWQQTKLVLSTSN
                     PNLGTTIPVLSAWNLQLYVPQVYTTMRNGNNAAKAYNRVQGMKAEEIAVSGDAEYSTN
                     NSTASEIVTSDISRYTTLNESQLFTSFEIELPYDIPSDGLNYSVAIKEEKIQATYKHY
                     AIPKLDKDAFLLAEINNWEALDLLPGDANIIMDNVYLGKSFIDPNSTLDTLNISLGRD
                     RRVSTKRMLMKEFSKTRFIGSNKIESFTYEVTVKNNKKEVVNMILKDQYPISRIKEVE
                     IKLEESSNAEINEELGTLNWKISLQPGEQKKYRFSYTVKYPKDKKIVNLR"
     gene            3295..4323
                     /gene="pheS"
                     /locus_tag="PLY81_02395"
     CDS             3295..4323
                     /gene="pheS"
                     /locus_tag="PLY81_02395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNF29109.1"
                     /translation="MNSLVQQLDIYKQEIISYNVSNADEVEAFRIKYLGTKGIIKHAM
                     GFMKDVPNEQKREAGQLLNEFKLMVEAKYESLKESFSASEQITNTIDFSLPGNIYNVG
                     TRHPLSIIRNKIVSIFQRLGFAIAEGPEIEDDWHNFGAMNLPEDHPARDMQDTFYVNQ
                     NPTWLLRTHTSSVQARVMENQKPPIRVICPGRVYRNETISARAHCFFHQVEGLYIDEN
                     VSFADLKQTLYFFVQEMFGKDVKVRFRPSYFPFTEPSAEMDISCLICNGDGCNVCKHT
                     GWVEILGSGMVHPKVLENFGIDSNKYTGFAFGMGVERICQLKYRVNDLRLYSQNDIRF
                     LKQFTSVI"
     gene            4503..5465
                     /locus_tag="PLY81_02400"
     CDS             4503..5465
                     /locus_tag="PLY81_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNF29110.1"
                     /translation="MKCSAIQLLNEKETRAGFGEGVYEAGKENENVVVLTADLAGSMK
                     IAPFIKDFPNRFIQVGIAEANMIGIAAGLTIGGKIPYTTTFANFSTGRVYDQIRQSVA
                     YSNKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVIVPCDFNQTKAATKAIA
                     NYNGPVYLRFGRPKWANFTTEDGSDFIIGKAQKLSDGNDVSIFACGHMVWKAIEAGKI
                     LEEKGISVEVINIHTIKPLDEDAIIQSIQKTKCAVTVEEHNIIGGLGDSIAQVAAKYL
                     PIPIEYIGTKDTFGESGKPTELLTKYGLDTPFIVDATERVIKRK"
     gene            complement(5484..6095)
                     /locus_tag="PLY81_02405"
     CDS             complement(5484..6095)
                     /locus_tag="PLY81_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNF29111.1"
                     /translation="MKVIILCNEIQEKEIISKPTATDVNVSFTNNKHTFYSTYADAYF
                     DLLADELNLEILRNDIPVFINSVIQTCKELPNNCIRLNAWNGFLKNEIIEIATLHHLE
                     YVEKVMNKLSWKYQVSSDDSGLIVPKIISMIVNEAYFALEDNISTKEEIDIAMKLGTN
                     YPYGPFEWSEKIGLKNIYFLLEKLSRTDNRYLPSKLLQTTALL"
     gene            6217..7374
                     /locus_tag="PLY81_02410"
     CDS             6217..7374
                     /locus_tag="PLY81_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNF29112.1"
                     /translation="MSKLLVNAQENIDQPHAKMVSKFAFTQLTGGLVILHATIDTSSS
                     VLNFLLDTGSGGISLDSTTAKRLGFSIYPTERMIRGIAGKRKLSFTYGHYLNFPEFVT
                     DTFSFHINNYDLISSAYGYKIDGVIGYSFLRKYIVKVDYDMQMVEVYKPGIIKYPRGG
                     HILKPDFTTLPFTYFSLGNNKKIKSRYLFDMGAGLCFLLNKQFVKDSTLFNKRTKFYA
                     TQAEGLGGKKTLELAVIKNVSLGPYYFKDVPVYIFDDEFKVISYPKNTGIIGNDLLRR
                     FNLIINYPDKIIYIKPNKKFVDAFDYSYTGLGIYYIDDKIIVTDVIKGSPGDKAGFEA
                     GDIIVAIDNDYSNNIQTVKHALQNAGAKLNVLISRNEKLEMMILDVKNIMH"
     gene            7450..7854
                     /locus_tag="PLY81_02415"
     CDS             7450..7854
                     /locus_tag="PLY81_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNF29113.1"
                     /translation="MNNLPKTIIMFDGVCNYCNKMVNFCIKHDKKKKLHYTSLQSNTG
                     MSLISYYNIPKSIDSIILIDESKVYYYSDAALKIARHLNPPIRFIYIFKFIPTFLRDA
                     LYKWVAKNRYKWFGKKDTCMIPSKEVKHLFLE"
     gene            7979..>9291
                     /locus_tag="PLY81_02420"
     CDS             7979..>9291
                     /locus_tag="PLY81_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29114.1"
                     /translation="MKKNVPFKALSFLFSLLVFSFASKAQLNPGSIAATSICTERNTD
                     APDIINNTLPSSGTAPYTYQWEAKIDYSGWAPVVSNGNGDSYSPGMLSYNTNFRRKVT
                     DANGNIAYSNEVRLYLTEGFTAGQIRLDNAVKDYNYYLPGSPIDLMIAAGARYGTDAY
                     SYQWETATALGGPWSAISGENGLTYQSPAINTVGSYFFRIKATDDNCGNIAYSDIIHI
                     QIVTTLPFFQGFFTHYYPCVFPNNTPSLLQGSLAIGGTGPYTYEWEKKGESDLTWQTI
                     ANSNTLTYQPGILTETTKFRRKATDANGNTGYTNESSIYIVTTVPNPGMIATNETAIA
                     PNAPYDAAINIQSASNFWNAGYAWQKSLDGGNTWTEINDYSYSTYFPPVTPTVVLTCY
                     RRSIREYCATNTRDTWTNTICIQPALPLTDGTISINGGNTACNTPG"
BASE COUNT         3444 a         1314 c         1486 g         3047 t
ORIGIN      
        1 caaaccattg tttctttatt ttatccaata cagttaaaca gtttagataa aggcttatgg
       61 cttatagcag ccacagcaat tattaatttt attgcaggaa ctatttgttt gcaaataggt
      121 aaaaaaaata attcaattgc attaaaagct acaggtaaac atttatatac cgatacctta
      181 tcaaccattg ttgttattgc aggcttatta attatgcttt actctaaaat gtattggtta
      241 gataaggtat tagcatttgc tataagtttt atcatattgt acaatggcta taaaattata
      301 cgaacatcca tggctggtat tatggatgag gcagatatgg aacttttaaa gcaaattatt
      361 aaagtattaa atgaaaacag aagagaaaac tggatagatc ttcacaactt tagagtaata
      421 aaatatggaa gattactaca tatagattgc catttaacta ttccatggta tcttaatatt
      481 catcaggcac acgaagaagt tgatttatta actaacctaa ttaaagaaaa atttggaggc
      541 ggaattgaac tttttgttca tacagatggt tgcttaaact ttagttgtag tatttgttta
      601 aaacaaaact gtattgaaag aaaacgtcca ttcttacata aaatagaatg gacgttagaa
      661 aatgttttat ctaatcaaaa acacaatagt gttatttaat ttcatacgtt tttaattcgt
      721 atttatcatt tatacttaca ccaatagttg aattattaat attagcagtg ttttctataa
      781 tacctatgtt tttagaaaaa taatattcaa tggttcctaa acttaaagga aaacctgcag
      841 cacttgcaga cagtgcagct tctactttaa taacattatt ataagttaca ccttctacaa
      901 ctcttgttgt acctttctct tttaatgtat aggtaatgtt tacagttgca gaactaacag
      961 gtacaccagg tatgcttaaa ccagtaagtg tttgggtggt ggtccaggta tcgttaacat
     1021 ttttatcatc ttttaaaata attaaatgaa cattttgacc agaaacttta attaaactat
     1081 gatagtcaga gcctacttta gcatagtatt gttcgttgcc attagagcca gtaataattt
     1141 tataagttat cccatctact actaaagtat ttgtacttac agtataagta acatcagaag
     1201 atgctcctcc agtagtaact ttatacttcc aatttgtacc tacattatta ggtaaataag
     1261 tatcggtaga tgtactacca ctgctgcttt ttttacaact gtttaaactt aaaattgcta
     1321 taaaagcaat aaatgcaatg agtgtttttt tcatttgtta ttaattaggg ttgcaaatta
     1381 tttatatttt tttataaata gaaatattta gaaacaagta tacgaaaagt ttcatacttt
     1441 atgttaactt ttcttttgct aaaaatttat gtatttattt aaaaaatacc atctattggg
     1501 tattatttaa taaaataaaa ccattatgaa attattagca gcatcttttt tagtacttat
     1561 cagttttaat agtattgccc aacaaaaaaa ttccatttta attacctctc aactatccag
     1621 tgcaaatgta ttttatggtt atggggcaga attaacacat aaagcaaaag ctaatattaa
     1681 aaagggacaa caagaaattg taattaaaaa catcagcact actgcagatg aaaatactct
     1741 tcaaattgcc actccagaaa atgtagtatt actaagttat aaacttgaaa ttaaacaaga
     1801 aggaatcaag cctaccaatc ctttaataaa aaaaatggag gatagcctta aaattctaca
     1861 aaagcaaata aatgcaaata atcaagactg gcaaatacaa gatgagcagc ttactaaaac
     1921 aagtaaaatt ttagaaactt atacaagttc aacaggtaaa aacataaaca cactagaagt
     1981 gctaaaatta attgattatt ataacggaaa agtgcaattt ttcagaaata gtatgtatgc
     2041 aattaaaata aaagtaaacg accttcaaga gcaagttttt gatataacca ataggctggt
     2101 agaacttaga aaaaaagagt atgcagaggc aaaaaaacca attgcaaact ttgtgcttca
     2161 agttatatct aaagaagcaa tggaagctga ttttgctatt tcttattaca ctcaaaatgc
     2221 aggctgggta ccaacttatg atatgagaat taaaagtata gacaataatt ttaagcttgc
     2281 atacaaagca ttaattagcc aaacaactgg tatagattgg cagcagacaa aactagtatt
     2341 aagtacaagt aatcctaatt taggaactac tattcctgta ttaagtgcat ggaatttaca
     2401 gttatatgta ccacaagttt ataccacaat gcgaaatggc aataatgctg caaaagctta
     2461 taatagagta caaggaatga aagcagaaga aattgctgtg agtggcgatg cagaatattc
     2521 taccaataat tcaactgcat ccgaaattgt aactagcgat attagcaggt acactacatt
     2581 aaacgaaagt caattattta caagttttga aatagagcta ccttatgata tacctagcga
     2641 tggattaaat tacagtgttg caataaaaga agaaaaaatt caggcaacct acaaacacta
     2701 tgcaatacct aaattagata aagatgcttt tttgttggca gaaataaata attgggaggc
     2761 tttagattta ttaccaggcg atgctaacat aattatggac aatgtttatt taggcaaatc
     2821 ttttattgac cctaattcta ccttagatac tttaaatatt tctttaggta gagatagaag
     2881 agtttcaacc aaacgaatgt taatgaaaga gttttcaaaa accaggttta ttggtagcaa
     2941 taaaatagaa agttttactt atgaagtaac agttaaaaat aataaaaagg aagttgtgaa
     3001 tatgatatta aaagatcagt atccaataag cagaataaaa gaagtggaaa taaaattaga
     3061 ggaaagcagt aatgcagaaa ttaacgaaga gttaggtact ttaaactgga aaataagctt
     3121 acagcctggc gaacaaaaaa aatataggtt tagttacaca gttaaatatc ctaaagataa
     3181 aaaaattgtt aacttaagat agctataaaa agcaacaggc tataaaacta tagcctgttt
     3241 ttattttaca atttttcata ctacaaatag gtttcatagc cttatcttcg cccaatgaat
     3301 agcttggtac aacaattaga tatttataaa caagaaatta ttagttacaa cgtatcaaat
     3361 gccgatgaag tagaagcatt tagaattaaa tatttgggca caaaaggcat tataaaacat
     3421 gcaatgggct ttatgaaaga tgtacccaat gaacaaaaaa gagaagctgg ccagctttta
     3481 aatgaattta aattaatggt agaagcaaag tacgaatcat taaaagaatc cttttctgct
     3541 tctgaacaaa ttactaatac tattgacttt tcattacctg gaaatattta caatgtagga
     3601 accaggcatc cattaagtat aataagaaat aaaatagttt ccatatttca aaggcttggt
     3661 tttgctatag ccgaaggtcc tgaaattgaa gatgactggc acaactttgg agcaatgaat
     3721 ttgccagaag atcatcctgc aagagacatg caagatacct tttatgtaaa ccaaaatcct
     3781 acatggcttt tgagaacaca tacaagtagt gtgcaagcta gagtaatgga aaatcaaaaa
     3841 ccacctattc gtgttatttg ccctggcagg gtttacagaa atgaaacaat tagtgcaaga
     3901 gcacattgtt tctttcatca agtagaaggt ttgtatattg atgaaaatgt aagctttgca
     3961 gatttaaagc aaacactata tttctttgta caagaaatgt ttggaaaaga tgtaaaagtt
     4021 agatttagac caagctattt tccttttaca gaaccaagtg ccgaaatgga tataagttgt
     4081 ttaatttgca atggcgatgg atgtaatgta tgtaaacata ctggctgggt tgaaatttta
     4141 ggaagtggta tggttcatcc taaagttcta gaaaattttg gaatagacag caataaatat
     4201 actggttttg cttttggtat gggagtagaa agaatttgcc agttaaaata tcgtgtaaac
     4261 gatttaagat tgtatagcca aaatgatatt aggtttttaa aacagtttac cagtgtaata
     4321 taagttaatg tttacggcgt aagtttaatt ctaattaaaa tctaaacaaa ttttaaaact
     4381 tacaagttgt ataaaaatct tttgaaataa attaaataat tgtacttcta atattttaac
     4441 taagcactgt ttttccttta catttgcacc actattgtta cccaatttta aaattttttg
     4501 taatgaaatg ctcagcaata caactactaa acgaaaaaga aacacgtgca ggttttggag
     4561 aaggtgttta tgaggcaggt aaagaaaacg aaaatgtagt agtactcact gcggatcttg
     4621 caggctccat gaaaattgca ccttttataa aagattttcc caatcgtttt atacaagttg
     4681 gtattgcaga agcaaacatg ataggcattg cagcagggtt aacaattggt ggtaaaattc
     4741 cttatactac tacgtttgct aactttagca ctggccgtgt gtatgatcaa attcgccaaa
     4801 gtgttgctta cagtaataaa aatgtaaaaa tttgtgcaag ccatgcaggg cttactttag
     4861 gagaagacgg agcaacacac caaattttag aagatattgg tttaatgaaa atgttaccag
     4921 gtatgactgt aattgtacct tgcgatttta accaaacaaa agctgcaact aaagctatag
     4981 ccaattataa tggtccggtt tatttacgtt tcggcagacc aaaatgggca aattttacta
     5041 cagaagatgg tagcgatttt attataggca aagcacaaaa attaagtgac ggaaacgatg
     5101 tttctatttt tgcttgtggc cacatggttt ggaaagcaat agaagcagga aaaattttag
     5161 aagaaaaagg cattagtgtg gaagtgatta atatacatac tataaaacca ttagatgaag
     5221 atgccataat tcaatctata caaaaaacaa aatgtgcagt aacggttgaa gagcataata
     5281 ttattggagg gttaggagat agtattgcac aagttgctgc aaaatattta ccaataccaa
     5341 ttgagtatat tggtacaaaa gatacatttg gcgaaagtgg taagccaact gagttactta
     5401 caaaatatgg attagatact ccatttattg tagatgctac tgaaagagtt attaaaagaa
     5461 aataagaatt gctatttgtt tcattaaagt aatgctgtag tttgcaaaag cttgctaggc
     5521 aaatatctat tatctgttct acttaatttt tctaataaaa aataaatatt ttttaaacca
     5581 attttctcac tccattcaaa cggcccataa ggataattgg ttcctaactt catagcaata
     5641 tcaatttctt ctttagtgct tatattatct tctaaagcaa agtaagcttc attaacaatc
     5701 attgaaatta ttttaggaac aattagacca gagtcgtctg acgatacttg atatttccaa
     5761 ctcaatttat tcattacttt ttctacatat tctaagtgat gtaatgttgc aatctcaata
     5821 atttcatttt tcaagaaacc attccatgca tttaatctta tacaattatt aggcaactct
     5881 ttgcaagttt gtataacact atttataaaa acaggaatat catttcttaa tatttcaaga
     5941 tttaattcat ctgccaataa atcaaaatat gcatctgcat aagtgctata aaaggtatgt
     6001 ttattgttgg taaaactaac attaacatcc gttgctgtag gtttagaaat aatttctttt
     6061 tcttgtattt cgttacaaag aattataact ttcattttct tacattatgc agcaaaagta
     6121 aagaaagtgc agtctgttga acggataata attttaaatt aattcatgaa ctatacatcc
     6181 tccattttaa aatattattt tctcttgttt ttgttaatga gtaaattatt ggtaaatgca
     6241 caagaaaata ttgatcagcc acatgctaaa atggtttcaa agtttgcttt tacacaatta
     6301 actggtggct tagttatttt gcatgcaact attgatactt cctcctctgt tttaaatttt
     6361 ttgttagaca ctggcagtgg tggaatatcc ttagattcta caactgcaaa gcgtttaggc
     6421 ttttctattt acccaacaga aagaatgatt aggggtatag caggaaaaag aaaactatca
     6481 tttacgtatg ggcattattt aaattttcct gaatttgtaa ccgatacttt tagttttcat
     6541 ataaataatt atgatcttat ttctagtgca tatgggtata aaattgatgg tgtaattggg
     6601 tatagttttt tacgcaaata catagttaag gtagattatg atatgcaaat ggtagaagtg
     6661 tataaacctg gcattatcaa gtatcctaga ggtgggcaca ttttaaaacc agattttaca
     6721 acattacctt tcacttattt ttcgttaggt aataacaaaa aaataaaaag tagatatttg
     6781 tttgatatgg gtgctggatt atgtttttta ttgaataaac agtttgtaaa agatagtact
     6841 ctatttaata aaagaacaaa attttatgca acacaagccg aaggtttagg aggtaaaaaa
     6901 acattagagc ttgctgtaat aaaaaatgtt agtttaggtc cttattattt taaagatgta
     6961 cctgtatata tttttgatga tgagtttaaa gtaatatctt atccaaaaaa tacaggaatt
     7021 attggaaacg atttattgag aagatttaat ttaattatta attatcctga taaaataatt
     7081 tatataaaac caaacaaaaa gtttgtagat gcttttgatt actcttatac agggttagga
     7141 atatattata ttgatgataa aataattgta accgatgtaa ttaaaggttc gccaggcgat
     7201 aaagcaggtt ttgaagcagg agatattatt gtagcaattg ataatgatta ttctaataat
     7261 attcaaaccg taaaacatgc attgcaaaat gcaggagcta aattaaatgt tttaatttct
     7321 cgtaatgaga aattagaaat gatgatttta gatgttaaaa atataatgca ttaacataat
     7381 agcataattt atatgcccca aaaagacaga aatggacatt ttttgttttt tattttataa
     7441 tttttgatga tgaataattt acccaaaaca attataatgt ttgatggcgt ttgcaactat
     7501 tgcaacaaaa tggttaactt ttgtataaag catgataaga aaaaaaaatt acattataca
     7561 tcgctacaaa gcaatactgg tatgtcttta attagctatt acaatatacc aaaatcaata
     7621 gattctatta tacttattga tgaaagtaaa gtttattact attcagatgc agctttaaaa
     7681 attgcaagac acttaaatcc tccaataagg tttatatata tatttaaatt tattccaaca
     7741 tttttaagag atgcattgta taaatgggta gctaaaaaca ggtataaatg gtttggtaaa
     7801 aaggacactt gtatgatacc ctccaaagag gtaaaacact tgtttttaga atgaaagtga
     7861 attttaaagc aactatttta tattaaattt gctcttatat agtcatccat atattcttag
     7921 ataagaatca tttactttat ccgtgaaaaa cgctgtttaa ttatttaata caagcgttat
     7981 gaaaaaaaat gtacccttta aagcattaag ctttttgttt tctttattag tgttctcttt
     8041 tgcatccaaa gctcaattaa accctggctc tattgcagcc acatctattt gtactgaaag
     8101 aaatacagat gcaccagata ttattaataa tactttacca agttctggaa ctgcacccta
     8161 tacttaccaa tgggaggcaa aaatagacta ctccggatgg gctccagttg taagtaatgg
     8221 aaatggtgat tcttatagtc caggaatgct ttcttataat accaatttta gaagaaaagt
     8281 aacagatgct aatggcaaca ttgcatactc taacgaagtg agattatatt taacagaggg
     8341 ttttactgct ggtcaaataa gattagataa tgcagtaaaa gattataatt attatttacc
     8401 aggtagccca atagacttaa tgattgctgc aggtgcaaga tatggcacag atgcatactc
     8461 ttaccaatgg gaaactgcaa ctgctttagg tggtccatgg tctgcaattt ctggagaaaa
     8521 tgggttaacc taccaatctc ctgcaattaa tacagtggga agttactttt ttagaataaa
     8581 agcaacagat gataattgcg gaaacattgc ttactccgat ataatccaca ttcaaatagt
     8641 tactacactt cctttctttc aaggcttctt tacacattac tacccatgtg ttttccctaa
     8701 caatacgcca agtttattac aaggtagcct agcaattgga ggaacaggtc cttatactta
     8761 tgaatgggag aaaaaaggag aatcagattt aacttggcaa acaattgcaa attccaatac
     8821 tttaacctac cagcctggaa tattaactga aacaactaag tttagacgta aagcaactga
     8881 tgcaaacggt aatacgggat acacaaatga aagttctatt tatattgtta caactgtacc
     8941 aaacccagga atgattgcaa ccaacgaaac tgctattgca cctaatgccc cttacgatgc
     9001 agcaatcaat attcaatctg ctagtaattt ttggaatgcc ggttatgctt ggcaaaaaag
     9061 tttagatggc ggtaacacat ggacagaaat taatgactat agttatagta cttatttccc
     9121 tccagttaca ccaacagttg tgttaacttg ttatcgtcgt tccattagag aatattgtgc
     9181 aacaaataca agagatactt ggacaaatac catttgtatt caaccagctt tacctttaac
     9241 cgatggaaca ataagcatta atggtggtaa tacagcttgt aatactccag g
//
LOCUS       DAOQHP010000040         9168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3822_length_9168_cov_3.250521, whole genome shotgun sequence.
ACCESSION   DAOQHP010000040 DAOQHP010000000
VERSION     DAOQHP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_9168_cov_3.250521"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLY81_02425"
     CDS             <1..270
                     /locus_tag="PLY81_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF29115.1"
                     /translation="DAMERKKKIKALEMESLQQNCTQIFIETPYRNNQLINDVLSICN
                     KQTKFCIAVNITSAAEKISTKSIESWKKNIPDIHKQPAIYLLNAF"
     gene            323..3736
                     /locus_tag="PLY81_02430"
     CDS             323..3736
                     /locus_tag="PLY81_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29116.1"
                     /translation="MLKKILSTLLIIVGFKAFSQTPIQMSSQVNNSYTETFADIDNWV
                     FKTTSEDGTFTAGVGAMCWRGVAINATGTIPSALKITHASTFFQIPPTGNGGYSSGVY
                     KGNQKLEILSTGTTNNTSSIAIDFFMDFTNVNAGTLSFDWASINNSSGNRSSSLKVYA
                     TVDGVNYIEITNAQVLNFTNNTPTAGSVVNVPLPTIFNNSATASLRFYYHNGTGGSSG
                     SRPRLSIDNVHVTAVPTTPCTAPTMQPTNFTLGAVTHNSINFSFTSAVPAPHNYFILM
                     STNSTLTALPVNGITYNVGDALADATVIGITNNNTFTASNLDLSTTYYFFLFSVNNSC
                     TGGPLYLTVNPLNGSATTTAGALPCAAPTTQPTNLSFTSITTNSIKGSFVPAANTDEY
                     LIIRSLSPTFTGILNNGTTYNGGNILGNGMVVTRTAGNTFTANNLTNGTTYYFFVFGL
                     NNQNCTGGAAYNSTNPLTNNTSTVSLPPCVTPTNQPSQLQLSASNHYVNGYFVAAPSA
                     DGYLIVRTAIATLSVTPQNGINYNAGDGLGNGTIIQNNASTAFIDINLQPSTTYYYHV
                     FAKNSVCNGNINYLSVNPLTGNTSTTAIAVNNWYYGNLHSHSSHSDGNQDNLSLTPID
                     DYAYAKNSLCMDFLGISDHNHASAGMSISNWQPGLNAASAATTSNFLALYGMEWGVIS
                     NGGHVLVYGSNQLIGWETNNYNIYVPKSDYLGTPETTGTTGLFRAINNLGGNTFATFA
                     HPSFSDYNGIANISYSATADSALVGSAVASGIAYSTNTTYSNPPSSFGYIDFYNRMLS
                     KGYHIGPTMDHDNHYTNFGRSNNNRLVVVAPSISSNDFYSAMRNRNFYASEDCDTRVV
                     FTLNNKKMGSILKDTVAPAISVYAYDPTNPGFTPTIKLMFGIAGSGVNPIQIYSSNSS
                     TLNYSDYELPIGSNAYYYADIQIAGNRTITAPIWFTKEALLPITLLSFNAYLNKEFNV
                     VNLNWQTTNEISNNYFILEKSNNGVEFFRLDSVKAKNTSGINNYAIVDKQPFDNITYY
                     RLKQVDMDGKYSYSKIIAVQLNNLLNEFTIQPNPIYNQASVKIKSYKKENTILKIMDM
                     QGRVVLQQLLQLNIGTQQININCNSLQKGLYNATLFLNNQIITRKVVKL"
     gene            complement(3753..5450)
                     /gene="recJ"
                     /locus_tag="PLY81_02435"
     CDS             complement(3753..5450)
                     /gene="recJ"
                     /locus_tag="PLY81_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNF29117.1"
                     /translation="MQKNWKIVEYDKALAETLQKELKINKTICKILVSRGIDNYTKAK
                     DFFRPTLEQLHSPWLMKDMDKATQRIIDAINKKEKILVYGDYDVDGTTSVACMYSFLK
                     AQHPFVDYYIPHRYKEGYGVSKAGIDFAAENNFSLIITLDCGIKSVDLINYAATLHLD
                     FIVCDHHMPDKILPNAIAILNAKQKDCNYPFKELCGCGVGFKLMQAIAEKLNLSKDSY
                     LQFVDLVAIAIAADIVPITGENRTLAYFGVKKVNENPNIGIKALISLAALEKSISINN
                     LVFIVAPRINAAGRMDDAKKAVQLFIETNVAKALEYAEMLHVDNKERKEADSSITEQA
                     LNLIKNNPTLINKKTTVVYNETWHKGVVGIVASRLIETYYKPTIVLTKSGNLITGSAR
                     SVVGYNLYDAIYSCKNYLTNYGGHFAAAGLSLLPEQLENFTNAFETVVSNTISPDLLI
                     PEIIIDAELAFDEITTTFYNIIMQMEPFGPNNMRPVFISRNVIDTGFSKIVKEQHLKI
                     SVKQNNIILNGIGFNMANKFSILEQNNQVDIVYTIDQNEWNGEKILQLKLIDLKPSK"
     gene            complement(5463..6425)
                     /locus_tag="PLY81_02440"
     CDS             complement(5463..6425)
                     /locus_tag="PLY81_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF29118.1"
                     /translation="MKLALKVLEKELNLFELHFKDAVKSRVALLDRIMEYIVKRKGKQ
                     LRPMFVLLSAKLGGEVNNTSYRAASLVELLHTATLVHDDVVDEANQRRGFFSINALWK
                     NKIAVLVGDYLLSKGLLLSLNNKDFKVLQILSTAVKEMSEGELLQIEKSRNLNLKEDV
                     YYEIIKGKTASLLASSCAAGASSTFNDYDMIEKMRLFGEKTGMAFQIKDDLFDYGNSN
                     IGKPTGNDIKEKKLTLPIIYTLNNCNKETRKKIIYIIKNKNKEKEKVNYVIEQVKIHK
                     GIEYATEKMNLFRDEALSILYSFPESETRNALEELVRYTTDREF"
     gene            6622..8145
                     /locus_tag="PLY81_02445"
     CDS             6622..8145
                     /locus_tag="PLY81_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29119.1"
                     /translation="MTSKKYTLLAMIFLLFSVASFAQDTDWGWDWKDSSKIPAKRMPQ
                     HNEFLNNNYPYPAKPRSQWEFGFAGGPSFLIGDVNPKIGFGGSLTARKAINHTFSLRA
                     GYFGSMTSGKPSTFGTSVGQTEYKNQKHGLGFDLVVSLNPISYYRGNPKMNWYLTAGY
                     EFVATRVQYKDALGAWRTFYAYQYPNSQSGIIGTVGGDKHNGRRAWTLLHGASVGAGV
                     AYKINNKTNIKLEQRFTKIVTGYDLLDPVKGGKSDDMYSFTSFGVNLNMGKTAKRVQP
                     LWWLNPNNFAYNELNKPKHMKMPKVVLDDADSDGITDQFDLEPNTPAGAKVDARGRAV
                     DTDGDGIPDYKDKEILTSQKCFPVNNDGVGTCPEPPCCKELRDEITKIKESGAATNKA
                     NTCEIGELPSIQFKGNSKLNKDAQAVLATVAQKIIANPNCKIKVTGYGSTSKSAQQAS
                     WEKVNAVIKYLVEKQGIAESRLIFTYGQDGDANTVDLQGTTEDGPNSVPAPHPNLRK"
     gene            8352..>9168
                     /locus_tag="PLY81_02450"
     CDS             8352..>9168
                     /locus_tag="PLY81_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29120.1"
                     /translation="MTKIKLILSLVVFIVFSNTINAQLGGVHNYSDSSYISPRDMKQQ
                     SDFMGNKGSFPSKPRDQWQFGIFAGYPYVDGDCATTFKGLGKGLQAYSYGFGISLRKA
                     MGYVFSVRGSFAYYNMLGLDYQPNSNFNNSQVIRNLYENAPRGYIHNHRTMAFTPSLE
                     GLVSLSNIMFHTKQKRWNIYGLFGYGALIYNTKMNMMKPDGSSYATEFQEVANAYTSG
                     VKRVKLRAMLREKLDGSYETMADVNDRRPGLNKNWHLRHCFTSGVGIEYRMGKR"
BASE COUNT         3142 a         1440 c         1430 g         3156 t
ORIGIN      
        1 gatgcaatgg agagaaaaaa gaaaattaaa gcacttgaaa tggaaagctt acaacaaaac
       61 tgtacacaaa tttttattga aacgccgtat agaaataatc aactaataaa cgatgtgtta
      121 agtatttgca ataagcaaac aaaattttgt attgcagtta atattacaag tgctgcagaa
      181 aaaatttcta ctaaatcaat agagtcttgg aaaaaaaata ttccagatat acataaacaa
      241 cctgctatat atttgttgaa tgccttttaa tgagattgga gaatattaat ttaaaataca
      301 ccatacaaat aaaataaaag tgatgctaaa aaaaatatta agtaccttat taattatagt
      361 tggattcaaa gcatttagtc aaacaccaat acaaatgtct tcgcaagtaa ataattctta
      421 taccgaaaca tttgcagata ttgataattg ggtatttaaa accacatcag aagatggaac
      481 atttactgca ggtgtgggtg caatgtgttg gcgtggtgta gcaattaatg ctacaggaac
      541 tatacccagt gcactaaaaa ttacacatgc atctactttt tttcaaatac caccaacagg
      601 taatggaggc tatagtagtg gcgtgtataa aggcaatcaa aagttagaga tactatctac
      661 tggtacaact aataatacta gttctattgc aatagatttt tttatggatt ttaccaatgt
      721 taatgctgga accttaagtt ttgattgggc ttctattaat aattcatctg gcaacagaag
      781 tagttcttta aaagtgtatg caaccgtaga tggggtaaat tatattgaaa taacaaacgc
      841 tcaggtttta aattttacaa acaatactcc aacagcaggt agtgttgtaa atgtgccatt
      901 acctacaatc tttaataatt ctgcaactgc aagtttaaga ttttattatc ataatggcac
      961 aggaggttca tctggcagca ggccaagatt aagtatagat aatgtacatg ttactgcagt
     1021 accaacaaca ccatgcactg caccaactat gcagcctaca aactttactt taggagcagt
     1081 tactcataat tctataaatt tttcttttac aagtgcagtg cctgcacctc ataattattt
     1141 tatattaatg agcactaaca gtaccttaac agcattgcct gtaaatggta ttacttataa
     1201 tgttggcgat gctttggcag atgcaacagt aattggtata actaataata atacttttac
     1261 tgcatccaat ttagatttat caactaccta ttattttttc ttattctctg taaataattc
     1321 ttgtacaggt gggcccttat accttactgt aaacccactt aatggaagtg caacaacaac
     1381 tgcaggtgct ttaccttgtg ctgcacctac aacacagcca accaatttat catttacttc
     1441 tataactacc aatagtatta aaggcagttt tgtacctgca gcaaataccg atgagtattt
     1501 aattattaga agtttatcgc caacttttac aggtatttta aataatggta caacttataa
     1561 tggtggtaat attttaggaa atggaatggt agtaacaaga accgcaggta atacttttac
     1621 agcaaataat ttaaccaatg gcacaactta ttattttttt gtttttggat tgaataatca
     1681 aaactgtacc ggtggtgctg catataattc tactaatcca ctaactaata atacttcaac
     1741 tgtatcctta cctccatgtg ttacacctac caatcaacct tcacaattac aattatctgc
     1801 atctaaccat tatgtaaatg gttactttgt agcagctcca tctgcagatg gttatttgat
     1861 agtaagaaca gctattgcaa ctttaagtgt aacacctcaa aatggtataa attataatgc
     1921 tggcgatggt ttaggaaatg gaaccattat tcaaaacaat gcatcaacag catttataga
     1981 tattaattta caaccctcta caacttatta ttatcatgtt tttgcaaaaa atagtgtatg
     2041 taatggaaat ataaattatt taagtgtaaa ccctcttaca ggtaatactt caacaactgc
     2101 cattgcagta aataattggt attatggaaa cttacattct cactcttcac attcagatgg
     2161 caaccaagat aatttatcat taaccccaat tgatgattat gcttatgcaa aaaattcatt
     2221 gtgtatggat tttctaggaa tatcagatca taaccatgca tctgcaggaa tgagtattag
     2281 taattggcag ccaggattaa atgctgcaag tgcagcaact acttccaact ttttggcttt
     2341 gtatggaatg gagtggggag ttattagtaa tggtggccat gttttggttt atggctctaa
     2401 tcaattaata ggatgggaga ccaataacta caatatatat gtacctaaaa gtgattattt
     2461 aggaacgcct gaaaccactg gtacaacagg tttgtttaga gctattaata atttgggtgg
     2521 taatacattt gcaacatttg cacacccttc attttctgat tataatggta tagcaaatat
     2581 ttcgtatagt gcaacagccg atagtgcatt ggttggtagt gctgttgcta gtggtattgc
     2641 ttatagtact aatactactt attccaatcc accaagttca tttggttata ttgattttta
     2701 caatagaatg ttaagtaaag ggtatcatat tggcccaaca atggatcatg ataaccacta
     2761 cactaatttt ggaagaagta ataacaacag attagtagta gtagcaccaa gcatttcaag
     2821 caatgatttt tattcagcaa tgcgtaatag aaatttttat gcatcggaag attgtgatac
     2881 aagagttgta tttacattaa acaacaaaaa aatgggaagt attttaaaag atactgtagc
     2941 acctgcaatt tctgtttatg cttacgaccc taccaatcct ggttttacac caacaataaa
     3001 attaatgttt ggtattgcag gtagcggagt taatccaatt caaatttatt catctaatag
     3061 ttctacatta aattattcag attatgaatt accaattggt tcaaatgctt attattatgc
     3121 agatattcaa atagctggta acagaacaat tactgcacca atttggttta ctaaagaagc
     3181 tttattgcct attacactgt taagttttaa tgcctatttg aataaagaat ttaatgtagt
     3241 aaatttaaac tggcaaacaa ctaacgagat aagtaataat tattttattc ttgaaaaatc
     3301 aaataatggg gttgagtttt ttagattaga tagtgtgaaa gcaaaaaata cttctggcat
     3361 taataattat gcaattgtag ataagcaacc ttttgataac attacttatt atcgtttaaa
     3421 acaggttgat atggatggca aatactctta ttctaaaatt attgctgtac aattaaataa
     3481 tctgttgaat gaatttacaa ttcaaccaaa tccaatttat aaccaagcta gcgtaaaaat
     3541 taaatcttat aaaaaagaaa atacaatttt aaaaattatg gatatgcagg gtagggttgt
     3601 attacagcaa ttattgcaat taaatattgg aacgcagcaa ataaatatta actgtaatag
     3661 tttgcaaaaa ggattataca atgcaacact atttttgaat aatcaaataa taaccagaaa
     3721 agtggtgaag ctataatagt aagatatagt agttacttac ttggttttaa atcaattaat
     3781 ttaagttgta atattttttc accattccat tcattttggt caatggtata aacaatatcc
     3841 acttggttat tttgttctag aatagaaaat ttatttgcca tattaaagcc aataccattt
     3901 aaaataatat tgttttgttt tactgaaatt tttaaatgtt gttcttttac aattttagaa
     3961 aagcccgtat ctattacatt tctagagata aaaacaggcc gcatattatt tggtccaaaa
     4021 ggctccattt gcataataat attataaaat gttgtggtta tttcatcaaa tgcaagttct
     4081 gcatcaatga taatttcagg tattaataaa tctggcgata ttgtgtttga taccacagtt
     4141 tcaaaagcat tagtaaaatt ttctaactgt tccggtaaaa ggcttaaacc tgcagcagca
     4201 aagtgtccac cataatttgt aagatagttt ttacaagaat aaattgcatc gtacaaatta
     4261 taaccaacaa cacttctagc acttcctgta attaaattgc cactttttgt aagtacaata
     4321 gttggtttgt aataagtttc aatcagcctg ctagcaacaa tacctactac acctttatgc
     4381 caggtttcgt tgtaaacaac tgttgttttt ttattaatta aagtaggatt attttttatt
     4441 aaattaagtg cttgttccgt aatactgcta tctgcttcct ttctttcttt attatctaca
     4501 tgaagcatct cggcatattc aagagctttt gctacattgg tttctataaa taattgaaca
     4561 gctttttttg catcatccat tcggcctgca gcattaatac gaggagctac aatgaaaact
     4621 aaattattta ttgatatact tttttctaat gcagctaaac ttattaaagc cttaatacca
     4681 atattaggat tttcatttac tttcttaact ccaaaatagg caagtgttct gttttcgcct
     4741 gttattggaa caatatctgc ggctatagct atagcaacca aatcaacaaa ctgcaaataa
     4801 ctatccttac ttaaatttag tttttcagca attgcctgca taagcttaaa gccaacacca
     4861 cagccacata attctttaaa tgggtagtta caatcttttt gtttagcatt taaaatagca
     4921 atagcattag gtaaaatctt atctggcatg tgatggtcgc aaacaataaa gtctaaatgt
     4981 agggttgctg cataatttat taagtcaaca ctttttatgc cacagtctaa agtaataatt
     5041 aacgaaaaat tattttcagc agcaaagtca atacctgctt tacttacacc atatccttct
     5101 ttataacgat gtggaatgta ataatctaca aacggatgtt gagcctttaa aaagctgtac
     5161 atacaggcaa cacttgttgt cccatcaaca tcataatcgc cataaactaa aattttttct
     5221 ttcttattaa tagcatctat tattctctgt gttgctttgt ccatgtcctt catcaaccat
     5281 gggctatgca attgttctaa agtgggtcta aaaaaatctt ttgctttagt ataattatca
     5341 atacctctac taactaaaat tttacaaatg gttttattaa tttttagttc tttttgtaaa
     5401 gtctctgcta aggctttatc atattctact attttccaat ttttttgcat gataaaaaaa
     5461 ttctaaaatt ccctatctgt tgtatatctc acaagttcct ctaaagcatt tctggtttca
     5521 gattcaggaa agctatacaa aatacttaag gcctcatctc taaataaatt cattttttct
     5581 gttgcatatt caatgccttt atgtattttg acttgttcta ttacataatt tactttctct
     5641 ttttctttat ttttattttt gataatataa attatttttt ttcttgtttc tttattacag
     5701 ttgttgagtg tatatataat tggtagagtt aatttttttt ctttgatatc attacctgta
     5761 ggcttaccaa tattgctatt gccataatca aacaaatcat cttttatttg aaaagccata
     5821 ccagtttttt ctccaaataa tctcattttt tcaatcatgt catagtcatt aaaagtagag
     5881 gaagcaccag ctgcacaaga tgaagctaat aaggatgcag ttttgccttt aattatttcg
     5941 taataaacat cttcttttaa atttaagttt ctagattttt caatttgtaa aagctctcct
     6001 tcactcattt ctttcactgc tgtagaaaga atttgtaaaa ctttaaaatc tttattattt
     6061 aaagagagta gtaacccttt tgataaaaga taatcgccaa ctaaaactgc aatcttattt
     6121 ttccataggg cattaataga aaagaatcct cttctttgat tagcctcatc aaccacatca
     6181 tcatgcacca aagtagcagt atgcagcaac tctactaaac ttgcagccct ataggatgtg
     6241 ttgtttacct cgcctcctaa ttttgcagaa agtaaaacaa acataggcct tagctgcttg
     6301 ccttttcttt ttactatata ctccattatc ctatctaata aagcaaccct gctttttact
     6361 gcatctttaa aatgcaattc aaaaaggttt aattcctttt ctaaaacttt taaggctaat
     6421 ttcatttaac ttggctaata agtttgcaat ataattatat atagaattga agaatttttt
     6481 attattggga agcattactt tccttaatat caggtttaaa ttaactttaa atattatttt
     6541 ttggtttttt agttgcttta attacttttg cttcgtatta aagtctaaaa attttcaata
     6601 tttaacttat aaattttcac tatgacaagc aaaaagtaca cattacttgc tatgattttt
     6661 ttattatttt ctgtagcaag ttttgctcaa gacacagact gggggtggga ttggaaagat
     6721 agttccaaaa ttcctgctaa aagaatgcct cagcacaatg agtttttaaa caataactac
     6781 ccttatcctg ctaaacctcg ttctcaatgg gagtttggtt ttgctggtgg tccatcattt
     6841 cttattggtg atgtaaaccc taaaattggt tttggtggtt ctttaactgc acgtaaagct
     6901 attaaccata ctttttcatt aagagctggt tattttggtt ctatgacttc tggtaaacct
     6961 agcacttttg gtacttcagt aggacaaaca gagtataaaa atcaaaaaca cggcttagga
     7021 tttgatttag ttgtctcttt aaatcctatt agttactaca gaggtaaccc aaaaatgaac
     7081 tggtatttaa cagctggcta tgaatttgta gcaacaagag ttcaatacaa agatgctctt
     7141 ggtgcttgga gaacatttta tgcttaccag taccctaatt cacaatctgg cataattggt
     7201 acagttggtg gagacaaaca caatggaaga cgtgcttgga ctttattaca tggtgcaagt
     7261 gttggtgcag gtgttgctta caaaataaac aacaaaacaa atattaaatt agagcaaaga
     7321 tttaccaaaa tagttactgg gtatgatttg ttagatcctg taaaaggtgg taaaagtgat
     7381 gatatgtata gctttactag ttttggtgtt aacttaaata tgggtaaaac agctaaaaga
     7441 gtacagcctt tatggtggtt aaatccaaac aactttgcat ataacgagtt gaacaaacca
     7501 aaacacatga aaatgcctaa agtggtgtta gatgatgctg atagcgatgg tattacagac
     7561 caatttgatt tagaaccaaa tactcctgca ggtgcaaaag tagatgctcg tggacgtgca
     7621 gttgatacag atggtgatgg tattcctgat tataaagaca aagaaattct tacttctcaa
     7681 aaatgtttcc ctgtaaataa tgatggtgtt ggaacttgtc cagaacctcc ttgttgcaaa
     7741 gaattaagag atgagataac taaaattaaa gagtctggtg ctgcaacaaa taaagcaaat
     7801 acttgcgaaa ttggtgaatt accaagcatt caatttaaag gtaattcaaa attgaataaa
     7861 gatgctcaag ctgtattagc aactgtagct caaaaaatta ttgcaaaccc aaattgtaaa
     7921 ataaaagtta ccggttatgg ctctactagc aaatcggctc aacaagcaag ttgggaaaaa
     7981 gtaaatgctg taattaaata cttagtagaa aaacaaggta ttgcagaaag cagattaatt
     8041 tttacttatg gccaagatgg agatgcaaat actgttgact tacaaggtac aacagaagat
     8101 ggaccaaata gcgttccagc tcctcatcca aatttgagaa aataattagt agaaactagt
     8161 aataatgtgc ataaaattgg cttcctgaaa aaaggaagcc ttttttatac acccatgtgg
     8221 aataatttat tgaaaattac cttgatttgc aacctattgt tacaattaat tgtctgtatc
     8281 ttgcgtttaa ttacttaatt gtaattttat ccaacctgtg aaagccgtaa aaaaagtttt
     8341 attttttaat tatgactaaa attaaactca ttctttcact ggttgtgttt atagtatttt
     8401 caaatacaat aaatgcccaa ttaggcggag tgcacaatta ttcagattct tcttatatct
     8461 caccaagaga tatgaaacaa caatctgatt ttatgggtaa taaaggaagt tttccatcta
     8521 aaccaagaga ccaatggcag tttggcattt ttgctggcta tccttatgta gatggtgatt
     8581 gtgctactac ttttaaagga ttaggtaaag gactgcaagc ttattcttat ggctttggta
     8641 ttagtttacg aaaagcaatg ggatatgttt tttccgttag aggttcattt gcctattata
     8701 atatgcttgg cttagattac caacctaact caaattttaa caatagccag gtaattagaa
     8761 atttatatga aaatgcccca agaggttata ttcataacca tagaacaatg gcatttactc
     8821 cttccttaga aggattagtt tcgttaagta atattatgtt tcataccaaa caaaaacgtt
     8881 ggaacattta tggcttattt ggatatggtg ccttaattta caatactaaa atgaatatga
     8941 tgaaaccaga tggtagttct tatgccactg agtttcaaga agtagcaaat gcatatacta
     9001 gcggtgtaaa aagagtgaaa ttaagagcca tgctaagaga aaagttagat ggatcttacg
     9061 agacaatggc agatgtgaac gatagaagac ctggtttaaa caaaaattgg catttaagac
     9121 attgctttac ctctggtgtt ggaattgaat atagaatggg taagagat
//
LOCUS       DAOQHP010000041         9147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3838_length_9147_cov_4.122415, whole genome shotgun sequence.
ACCESSION   DAOQHP010000041 DAOQHP010000000
VERSION     DAOQHP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_9147_cov_4.122415"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLY81_02455"
     CDS             complement(<1..773)
                     /locus_tag="PLY81_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNF29121.1"
                     /translation="MNTIQAIVIAIIEGLTEFLPISSTAHMGFTAHLLGLNNNDEYLK
                     MYQVSIQFGAILAVVVAYWSKFFQFKNIKFYSKLIIAVIPALVLGKLFDDKIEMVLGN
                     QIAISVVLVLGGIVLLFVDNWFKQPKITDEEQISYKKSLTIGFFQCLAMMPGTSRSAA
                     SIIGGMALGLSRKVAAEFSFFLAVPTMLAVTLYSVFIKTWGEGTVQQAKGYTMITANS
                     NNFTNFIIGNIVAFIVALLAIKFFIALLNKFGFKMWGWY"
     gene            906..2732
                     /gene="mutL"
                     /locus_tag="PLY81_02460"
     CDS             906..2732
                     /gene="mutL"
                     /locus_tag="PLY81_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNF29122.1"
                     /translation="MTDIIELLPDSIANQIAAGEVIQRPASAVKELLENAVDAGATEI
                     KLLIVDAGKSLVSVIDNGKGMSETDARMAFERHATSKIKKIDDLFSIKTMGFRGEALA
                     SIAAVAQVELKTKREKDELGTCIEIENSVVKNQEPCATSIGTTISMKNLFFNVPARRN
                     FLKSNASEMKHIIDEFIRVAMSFPEIVFTLSSNGQQIYYLEAGSLKQRIIQILGNNYN
                     AKLVSVNEQTDYLNIYGFVGKPETAKKTRGDQYFFVNNRFIKSAYLNHAVNTAFENML
                     AKDSFPSYVLFIDLDPSQIDINVHPTKQEIKFEDEKIVYAFVQAAIKHALAQFSIAPS
                     LDFTLNADIQNLDAVHKPFTTEHKQQATASNLFKTFTGKHQAHKIEPNEKSELKHWKD
                     FFTTRPIVEINNEPEVEQKIQYNQLQNVESLFIQLHKTYILTTNQAGFLLVHQQHAHE
                     RVLYEKYSAYSHNKQIATQKNLFPITFELATPDAILLEDLMPEIITIGYQIEPFGNNT
                     FIIQGTPADVGTGNEKHSLELLLEQFKHFSSDIKYSKREKLVRCISRQQAIKAGTTLS
                     ITEMQNLLESLFQCTTPNVTPTGNPTYIEFKEDYLDRMFGKM"
     gene            complement(2787..3707)
                     /locus_tag="PLY81_02465"
     CDS             complement(2787..3707)
                     /locus_tag="PLY81_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNF29123.1"
                     /translation="MSLVVVGSMAFDAIETPFGKSDKIIGGAGTYIAFCSTYFTPVKQ
                     ISVVGGDFPEDELDELSKRNVCLEGVQIKKDEKTFFWSGRYHMDMNSRDTLVTELNVL
                     GDFKPIVPESYQDCEFLMLGNLAPAVQLSVIEQLKNKPKLIALDTMNFWMEVAMDDLK
                     KVLEKIDVLIINDSEARQLSGDYSIVRAAKKIMTMGPKYLVIKKGEHGALLFHENNVF
                     VAPALPLEDVFDPTGAGDTFAGGFIGHLAKTKDISFENMKTAIIVGSAMASFCVEKFG
                     TERLRNLSKDAIAERINEFVQLVHFDVSLV"
     gene            4103..4297
                     /locus_tag="PLY81_02470"
     CDS             4103..4297
                     /locus_tag="PLY81_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29124.1"
                     /translation="MKTQQTNLLQPLTNNEIVKLTTTQNEIVAVADNNNNKVFSSADL
                     WNLRRSRKSVTPIRRAAFIW"
     gene            4355..4693
                     /locus_tag="PLY81_02475"
     CDS             4355..4693
                     /locus_tag="PLY81_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNF29125.1"
                     /translation="MDIQNTQSQMRKGVLEFCILSIIRQGEVYPSDIADKMKAANLQI
                     LEGTLYPLLTRLKNAGYLTYRWVESNSGPPRKYFVMTDKGLDFYNELGKTWQELADAV
                     ATLTQQNNNS"
     gene            4726..6879
                     /locus_tag="PLY81_02480"
     CDS             4726..6879
                     /locus_tag="PLY81_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNF29126.1"
                     /translation="MKKVININFQGRVIPIEENAYDILKRYIESLGKLFANEEGKEEI
                     INDIEGRIAELFGEVLKKGSTCITAEDVNTIINSMGRPEDFDEDISQPNADKQQHQNY
                     EHQNSYSNTSDTKKMYRDENNKVLGGVCSGIANYFGIDPVVTRVIFVIFFGVMFVPYI
                     ILWVAIPSSSTQVIGSRRKRLFRDPDDKILAGVCSGISHYFGISVWIPRLLFLIPFIS
                     FIFRFNTWDIWDFPNFLKISFSPGALFIYIILWMILPEAKSSADKLEMKGEKVDLNNI
                     KNTIQSDIENFAKKAEKFGETISEKGAEFGNTVQEKGKQFSTEANTIFKKRGKGLGDI
                     IVLIVKIFAYFILGSVLLAIVVSLFGLGIVASGFLPLKGYIISTGWQNVFAWGTLLLF
                     IWVPIIAIVVFIVRRLAKIKGKSCLVRYTFSALWVLGLVCFINLLVSLSKDFKYRNNA
                     MEEQVLLTNPTVNKLEINPNNLTNYYNNNWFDMAPFADVDEDSSVIKNVRIRITKATN
                     DSFSVKMVKLSNGNSKKQANELAQKINFSIEQKDTLLYLGRGITINPKDKFRNQNVII
                     SIAVPIGKKIIIKRNAGWSWNRNIHVSFFDDSDFDWYNLDGSELAWKHNVEYIMTNEG
                     LKRTNNKYDEDDDDDFRMNEKDYLQKEYEEAQQKADRLKQKLDNNTKDSTGLDEKKYR
                     YEPTTPKTSNTPTKSTVQIPNNNAISKNILIKFGI"
     gene            complement(7207..7608)
                     /locus_tag="PLY81_02485"
     CDS             complement(7207..7608)
                     /locus_tag="PLY81_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF29127.1"
                     /translation="MKKYLIDTNIAVFYLRGKHNLESKFDQVTADDCYISEITLAELK
                     FGVEKSEKPEKNRKVLENFLTGVQVLPIFHSLDLYAKEKARLQKAGTPIDDFDLLIGV
                     TSITHNLTMVTNNTNHFKNIREIDLEDWTQA"
     gene            complement(7605..7853)
                     /locus_tag="PLY81_02490"
     CDS             complement(7605..7853)
                     /locus_tag="PLY81_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29128.1"
                     /translation="MGTTAEKNTTLIDGYVGMLVNLSTTNKLDLISKLTASVKTDLTN
                     KKSSFKKAYGAFESKKSAEEIIEEIRNSRVSTRQIEAF"
     gene            complement(8055..8216)
                     /locus_tag="PLY81_02495"
     CDS             complement(8055..8216)
                     /locus_tag="PLY81_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF29129.1"
                     /translation="MRTFHAQKYTTMVIIAFYNLKGGVGKTASAVTFAYLAAANGLKT
                     LVWDLDPQV"
     gene            8266..8955
                     /locus_tag="PLY81_02500"
     CDS             8266..8955
                     /locus_tag="PLY81_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29130.1"
                     /translation="MAIINIANNMGYKNAMFLLSILISFVLYGQKKIIFKDNFVNNKN
                     RWKLEKADDEFSVRIKDKALYFKKNHRNAVNNGCLWFEKNIRKFKAEKNFIITFYAKI
                     LSSDDVSNGFDFQWGKIEEDTNTSSSISTSLYQLDFGLTDMRLSKFDNINGWTYYDWS
                     DSYTETLNYTLQYGILYKYEIIQRNDTLTVLINDKIVYALPIKPQDGKSIGVQQCLKS
                     HWLLSRLSIKQ"
BASE COUNT         3392 a         1387 c         1381 g         2987 t
ORIGIN      
        1 ctgtaccaac cccacatttt aaaaccaaac ttatttaata atgcaataaa aaatttaatt
       61 gcaagcagag caacaataaa agcaactata ttgcctataa taaaattagt aaaattattg
      121 ctgttggctg taatcattgt ataacccttg gcttgttgca ctgtaccttc tccccaggtt
      181 tttataaaaa cagaatacaa tgttactgcc aacatggttg gtactgctaa gaaaaaagaa
      241 aactctgcag caacttttct acttaacccc aatgccatgc caccaataat agaagctgca
      301 ctacggctag taccaggcat catagccaag cattgaaaaa agccaatagt taaacttttt
      361 ttatagctaa tttgctcttc atctgtaatt tttggttgct taaaccaatt atcaacaaat
      421 aataatacaa tacctcctaa aactaaaaca acacttattg caatttgatt gcctaaaacc
      481 atttcaatct tatcatcaaa taattttcct aaaaccaaag ctggtattac agcaataatt
      541 agtttactat aaaatttaat atttttaaac tgaaaaaatt tactccaata agctactaca
      601 actgctaaaa ttgctccaaa ctgaatactc acctggtaca ttttcaaata ttcatcgtta
      661 ttatttaaac ctaataaatg tgcagtaaaa cccatgtgag ctgtagatga aataggcaaa
      721 aactcggtta gcccttctat tattgcaata acaatggcct gtatggtatt catttaatag
      781 cttttagtaa taaacaaaga aaacagtgtt tgagaactat tcaaaatgtt tattgtattt
      841 taaaataaag tttataaaag aacataaata aaaaactact tttgaaccca tttaataaaa
      901 ttgtagtgac agatattatt gaacttttac cagatagtat tgctaaccaa attgcagcag
      961 gagaagttat tcaacgacca gcaagtgccg taaaagaatt gttagaaaat gcagtagatg
     1021 caggtgccac agaaataaaa ttattaatag tagatgcagg aaaaagcctt gtatcggtta
     1081 ttgataatgg aaaaggaatg agtgaaacag atgcaagaat ggcttttgaa agacatgcca
     1141 ctagtaagat aaaaaaaatt gatgaccttt ttagtattaa aacaatgggt tttagaggag
     1201 aggctttagc aagcattgct gctgttgccc aggtagagct taaaaccaaa agagaaaagg
     1261 atgaattagg tacatgtatt gaaattgaaa atagtgttgt aaaaaatcaa gagccttgtg
     1321 ctaccagtat tggtactacc attagtatga aaaacctatt ttttaatgtg cctgctcgta
     1381 gaaatttttt aaaatccaat gcttcagaaa tgaagcatat catagatgag tttattaggg
     1441 ttgcaatgtc ttttcccgaa atagtattta ccttatctag caatggtcag caaatttatt
     1501 atttagaagc aggtagttta aaacaaagaa ttatacaaat tttaggtaat aattacaatg
     1561 ctaaattagt aagtgtaaat gaacaaacag attacctaaa tatatatggc tttgtaggta
     1621 aaccagaaac tgctaaaaaa acaagaggcg atcagtattt ttttgtaaac aaccggttta
     1681 taaaaagtgc ttatttaaat catgctgtaa acacagcttt tgaaaatatg ttagctaaag
     1741 acagttttcc aagttatgtg ttgtttatag atttagatcc atcgcaaatt gatattaatg
     1801 tacaccccac taaacaagaa ataaaatttg aagatgaaaa aattgtgtat gcatttgttc
     1861 aagcagctat aaaacatgca ctggcacaat ttagtattgc acccagttta gattttactt
     1921 taaatgccga tatacaaaat ttagatgcag tgcataaacc ctttacaacc gagcataagc
     1981 aacaagctac tgcctctaac ctatttaaaa catttactgg taaacaccag gcacataaaa
     2041 tagaaccaaa cgaaaaaagt gaattaaaac attggaaaga tttttttact actcggccaa
     2101 ttgttgaaat aaacaatgaa cccgaagtgg agcaaaaaat tcaatacaac caattacaaa
     2161 atgttgaaag tttatttatt caattacata aaacatatat attaacaact aaccaggctg
     2221 gatttttatt agtacatcag caacatgcac acgaaagagt tttgtacgaa aaatattctg
     2281 cctattctca caataagcaa attgctactc aaaaaaatct ttttcctata acatttgaat
     2341 tagcaacccc agatgctatt ttgttagaag atttaatgcc cgaaattatt actattggtt
     2401 atcaaataga gccttttggt aacaatactt ttattattca aggcacccca gcagatgttg
     2461 gtacaggaaa tgaaaaacac tctttagaac ttttactaga acaatttaaa cactttagta
     2521 gcgatataaa atacagtaaa agagaaaaac tagttcgttg tattagtagg caacaagcaa
     2581 taaaggcagg tacaacctta tctattacag aaatgcaaaa tttattagaa agtctttttc
     2641 aatgtacaac tccaaatgtt acacccacag gtaatccaac ttatatagag tttaaagaag
     2701 attatttaga cagaatgttt ggaaaaatgt aataaaaaaa tccgtaaagc tactctacgg
     2761 atttttagtt atttgtaaat aataaattaa actaggctta catcaaaatg tactaattga
     2821 acaaattcat taattctttc tgcaattgca tctttgctta aatttcttaa tctttcagta
     2881 ccaaattttt caacacaaaa acttgccatt gcactaccta caataatagc tgttttcata
     2941 ttttcaaaac taatgtcttt tgttttagct aaatgcccaa taaatcctcc agcaaaagta
     3001 tctcctgcac cagttggatc aaaaacatct tctaatggta aggctggtgc tacaaataca
     3061 ttgttttcgt gaaataataa agctccatgt tcaccttttt taattacaag gtatttgggt
     3121 cccatggtca ttattttttt tgcagctcta actattgaat aatcaccact aagttgcctt
     3181 gcctcactat cattaataat taatacatct attttttcta aaactttttt caaatcatcc
     3241 atggcaactt ccatccaaaa gttcatggta tctaaagcaa ttagtttagg tttgttttta
     3301 agctgctcaa taacacttaa ctgtactgca ggggctaaat tacctaacat taaaaactca
     3361 caatcctgat aactttcagg aactatgggt ttaaaatctc ctaatacatt caactctgta
     3421 accagggtat ctctactatt catatccatg tggtaacgcc cactccaaaa gaaagttttt
     3481 tcatcttttt taatttgaac tccttctaaa caaacatttc ttttacttaa ttcatctaat
     3541 tcatcttctg gaaaatcgcc acctactaca cttatttgtt ttacaggtgt aaaatatgtt
     3601 gaacaaaaag caatataggt gcctgctcca cctattattt tatcactttt accaaaaggg
     3661 gtttctattg catcaaaagc catagaacca accacaacta aagacataaa ttaataattt
     3721 ttattttttg atggcagcga aactaaagca ttgttcttaa ttagatagtt tttatgtttt
     3781 tttcttacaa attctacttc caaaatttaa aatttttatg ttttcaagta aaattttgca
     3841 attttcatca tgctatttta ccgctcatca taatactatg tattgcatag taccatgtaa
     3901 ggctttggtt gaatgcctat aaacattgag tttcagagct atttttatca ttatccttgg
     3961 caaactactt aaataaggat agtattatgc attgcatagt attaaaaaaa atcccatctt
     4021 tgtgttgtca atgtaaaaaa agggtataaa cccccaaaac attaaaaaaa caaaaaattt
     4081 aaaaaattaa aaaaatacta caatgaaaac tcaacaaaca aatcttttac aacctttaac
     4141 taacaacgaa atagttaaac ttactaccac tcaaaatgaa attgtggcag ttgctgataa
     4201 caataataat aaagttttta gttctgcaga tttatggaat ttaagaagat ctagaaaatc
     4261 tgttactcct atccgccgtg cagcatttat ttggtaatac tttattctat tacaaaacac
     4321 cggtattaaa tttttaacta ttaatattac tataatggat attcaaaata cacaaagcca
     4381 aatgcgaaaa ggagtattgg agttttgtat tttatctata atacgccagg gagaagttta
     4441 cccaagtgat attgcagaca aaatgaaagc agctaacctt caaattttag aaggcacatt
     4501 ataccctttg ctaaccagat taaaaaatgc aggatattta acttatagat gggttgaaag
     4561 caatagcgga ccaccccgaa aatattttgt aatgacagat aaaggtcttg acttttacaa
     4621 cgaattaggc aaaacctggc aagaacttgc agatgcagtg gccactttaa cccaacaaaa
     4681 caacaactca taaaactaac tttaaccaaa taaaccttac aaagtatgaa aaaggtaatt
     4741 aatataaact ttcaaggcag agtaattcca attgaagaaa atgcctacga tattttaaaa
     4801 agatacattg aaagcttagg taaacttttt gctaacgaag agggcaaaga agaaataata
     4861 aatgatatag aaggaagaat tgcagagctt tttggcgaag tattgaaaaa aggttctacc
     4921 tgcattacag cagaggatgt aaatactatt attaatagca tgggtaggcc agaagatttt
     4981 gatgaagata tttcgcagcc taatgccgat aagcaacagc atcaaaacta tgagcaccaa
     5041 aactcttata gtaatacaag cgataccaaa aaaatgtata gagatgaaaa caataaagta
     5101 ttagggggtg tttgcagtgg tatagctaat tattttggta ttgatccggt ggtaacccgt
     5161 gtaatttttg tgatattttt tggagtaatg tttgtgcctt acattattct atgggttgca
     5221 attccaagca gttccactca agtaattggc agtagaagaa aaagactttt tagagatcct
     5281 gatgataaaa ttttggctgg cgtttgtagt ggtatcagtc attattttgg gataagtgtt
     5341 tggattccga gattgctttt tttaataccg tttatttcct tcatatttcg ttttaatact
     5401 tgggatattt gggattttcc taatttctta aaaatatctt ttagcccagg agcattattt
     5461 atatatatta ttttatggat gattttaccc gaagcaaaat ctagtgccga taaactagaa
     5521 atgaaaggtg aaaaagttga tttaaataat ataaaaaata ctattcaaag cgatattgaa
     5581 aattttgcaa aaaaagcgga aaaatttggc gaaacaatta gcgaaaaagg agcagagttt
     5641 ggtaacacag ttcaagaaaa agggaagcaa ttttctaccg aggcaaatac aatttttaaa
     5701 aaaagaggta aaggcttggg cgatataatt gtacttattg taaaaatatt tgcatatttt
     5761 attttaggct cagtgttatt ggcaattgtt gtatcattat ttgggttagg tattgtagca
     5821 tcaggatttt taccacttaa aggttatatt atttcaactg gttggcaaaa tgtttttgca
     5881 tggggcacat tgttactatt tatttgggta cccattattg ctatagttgt ttttatagta
     5941 cgcagattgg caaaaattaa aggtaaaagt tgtttggtaa gatatacctt ctctgcacta
     6001 tgggtattag gtttggtttg ctttataaac ttacttgttt ctttaagtaa agattttaaa
     6061 tacagaaata atgctatgga agaacaagtt ttacttacca atcctacagt aaataaatta
     6121 gaaattaatc caaataactt aaccaattac tacaataata attggtttga tatggcccca
     6181 tttgcagatg tagatgaaga tagcagtgta ataaaaaatg taagaataag aattacaaaa
     6241 gcaaccaacg atagtttttc ggtaaaaatg gtaaaactaa gtaatggcaa cagtaaaaaa
     6301 caagctaatg agttagcaca aaaaataaac ttttctattg agcaaaaaga tacactattg
     6361 tatttaggaa gagggataac tattaaccca aaagataaat ttagaaatca aaatgtaata
     6421 attagtatag ccgtaccaat tggtaaaaaa ataattatta aaagaaatgc aggatggagt
     6481 tggaatagaa atattcatgt tagcttcttc gacgattcag attttgattg gtataattta
     6541 gatggctctg aacttgcttg gaaacataat gtagaataca taatgaccaa tgaagggtta
     6601 aaacgtacca ataataaata cgatgaggac gatgacgatg attttagaat gaatgaaaaa
     6661 gattatttac aaaaagaata cgaagaggca caacaaaaag ccgatagatt aaaacaaaag
     6721 ttagataata acacaaaaga cagcaccggt ttagatgaaa aaaaatatag gtatgaacct
     6781 actaccccta aaacatccaa cacacctact aaatcaacag tacaaatacc aaataacaat
     6841 gctatttcta aaaatatttt aataaaattt ggtatataaa taatttaaag ttggttgaag
     6901 ttgggaataa aagaaccttg tactttaatt agtgcagggt tctgtttttt taagctaatt
     6961 tttgaaatga atagaataag ttgtagttta attttaatct aaaatttctt ttttattata
     7021 gttctgccat tgtgtaagta taagagtaaa tacacctact agtatttaaa ctttttccta
     7081 aaaaatataa aaaaatattt tacaataaaa aaataaaaca tcttgtaatt tcaaatcgta
     7141 ctaatcttcg gaaagcttac aacaagaaga tttagagtat ttgtttagtt aggaagaaga
     7201 aaaaaactag gcttgtgtcc aatcttccaa atcaatttca cgaatatttt tgaaatgatt
     7261 tgtattgttt gtaaccattg tcaaattatg tgttatagaa gttacgccaa tcaacaaatc
     7321 aaaatcgtca ataggcgttc ctgctttttg aagtcttgct ttttcttttg cgtacaaatc
     7381 taatgaatgg aaaataggaa gtacttgaac accagttaaa aagttttcca atacttttcg
     7441 gttcttttca ggtttttcac ttttttcaac accaaatttc aattctgcca aagttatttc
     7501 tgaaatataa caatcatctg ccgtaacttg gtcaaacttt gattcaagat tatgcttgcc
     7561 tcttaaataa aaaacggcaa tgtttgtatc aatcaaatac tttttcaaaa tgcttctatt
     7621 tgtcttgtag aaactctgct gttacgaatt tcttcaataa tttcttctgc agattttttt
     7681 gattcaaaag caccatacgc ttttttgaat gacgattttt tgtttgttaa gtcggtttta
     7741 acagaagctg taagtttaga aataaggtca agcttgttgg tagtgcttaa attaacaagc
     7801 ataccaacat aaccgtctat aagcgtagta tttttttctg ctgttgttcc catttgccaa
     7861 atgtacaaaa agttataaaa aatggaagaa aataaaattg taatagtttg tcaagaatat
     7921 ttagttcctt tttacttctt ccccaacagt ttcattaaat cttctatttg tttggtaagt
     7981 cgcactattt ctgcatcttt tgaagcaagt agtttttcat aagcagtttt tgaatctgca
     8041 taaaaatttt caaattacac ttgagggtct aaatcccata ccaaagtttt taaaccattg
     8101 gctgctgcta agtatgcaaa ggttactgca gaagcagttt taccaactcc tccttttaaa
     8161 ttataaaaag ctattataac catagttgta tatttttgag catgaaaagt acgcacttgt
     8221 tggctctaca caaaattttt agaatagaag ttgtagtatt tttctatggc gataataaat
     8281 atagcaaaca atatgggata taaaaatgca atgttcttat taagcatctt aatttctttt
     8341 gttttatatg gacaaaaaaa aataattttt aaggacaact ttgttaataa taagaatcgt
     8401 tggaaattgg aaaaagcaga tgacgaattt tctgtaagaa ttaaagataa agcactttat
     8461 tttaaaaaga atcatagaaa cgctgttaat aatggttgtc tatggtttga aaaaaatatt
     8521 cggaagttta aagcagaaaa aaattttata attacttttt acgctaagat attgagctct
     8581 gacgatgttt ctaatggatt cgattttcaa tggggtaaaa tagaagaaga taccaatact
     8641 tcatcaagca tcagcacatc tctgtatcaa cttgattttg gactaacaga tatgcgttta
     8701 tcaaagtttg ataatattaa tggttggaca tattatgatt ggtctgatag ttatactgaa
     8761 acattaaatt atacattgca gtatggtatt ctatataaat atgaaatcat tcaaagaaat
     8821 gatactttaa ctgtattgat taatgataaa attgtttatg ctttacccat caaaccacaa
     8881 gatgggaaaa gtattggtgt tcaacaatgt ttgaaaagcc attggttatt aagcagacta
     8941 agtattaaac aataaagtat tttaactgta aaatttttct tcaatactta attatataaa
     9001 atcttctaca tgaaaaaagt attgaaaatt ttcggctacg ccgtgctggg cctgctgatc
     9061 ttaatcggct gcctggctgc ctatatccaa tttgcacccc tgcccacata cgccgtcaaa
     9121 gcgcccgacc tgaccgttac gcccgac
//
LOCUS       DAOQHP010000042         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3853_length_9121_cov_3.910214, whole genome shotgun sequence.
ACCESSION   DAOQHP010000042 DAOQHP010000000
VERSION     DAOQHP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_9121_cov_3.910214"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PLY81_02505"
     CDS             <1..1461
                     /locus_tag="PLY81_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNF29131.1"
                     /translation="KHISEMQKYLSSQSNIKSGAVWKGRENVLDAAMKQSDRWRNMKS
                     EGADEETIKKSFYEKTKMKVFSWNKNREADTVMTPFDSIKYSRQMLQAGFMVMDPITG
                     EVKAWVGGIDFKNYKYDHVNFNTKRQVGSTIKPLLYGLAIEEAGLTPTSMVQDVQQSF
                     GSYGLVPATSKTCTGATMPMAQALAGSRNCASAYIIKQLDNSGNNGARRFVDFLKRCD
                     FKTKLDAYPSMALGACEISLYEMLQGYTMFPGRGFNVQPMFITRIEDRNGNVLETFTP
                     KRKEVISDVTAYSVIKMMEGVMEFGTGRRIWSYDVKGKIAGKTGTTNDNSDAWFIGYT
                     PQILAGTWVGCDDRFIRFNSTAIGQGASAALPIWAYFYEKASRDPACGLDVTSTFAKP
                     EALTNDIIIDGISGFTPVPGGETEDGGAGTSSEYGEESSEYNDDSTTPTQTNETQNSD
                     NKNNQDNKNKTEKKQPPATLPNKDQKPKAVIPKKNT"
     gene            complement(1458..2396)
                     /locus_tag="PLY81_02510"
     CDS             complement(1458..2396)
                     /locus_tag="PLY81_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNF29132.1"
                     /translation="MIQYFKNIEGKTVEISKPENDIWVNLVPPFKEEDFTDLSNNLDI
                     PIEFLRDSLDIDERSRYELEDNVKFIVIKTPAENNSFNDSDAFYITIPICIILTHNHI
                     VTVNSFDNGAIKKFLNTFQNRQTDKRNMMVLKIFEKIVQDYLGYLKQINNRRNQYEQS
                     LYDSNSNEDLLNLMRIQKSLTYFVTALRSNEMLMMKLERTNFLGLNEDDRELLRDLIV
                     DTSQALEMANIYANILSSTMDAFASIISNNLNNVMKRLTSITIILSLPILVTSIYGMN
                     VEIPYQHSSHAFYIPVILSLIISVVIGWYFLKKKWF"
     gene            complement(2492..3265)
                     /gene="dapF"
                     /locus_tag="PLY81_02515"
     CDS             complement(2492..3265)
                     /gene="dapF"
                     /locus_tag="PLY81_02515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNF29133.1"
                     /translation="MTIFFSKYQGTGNDFIIIDNRENKINLSYQQINFLCNRKFGIGA
                     DGLMLLNSHSLYDFEMIYYNADGHESSMCGNGGRCITQFAHDIGLIKDKYLFTAIDGL
                     HEAVVKDIVYLKIQDVKGVENEHNEVFILNTGSPHYIKILPSITDLDVYTEGKKIRYN
                     SRFEKEGINVNFVQRINDNTIKVRTYERGVEDETLSCGTGVTAAALVNATTVGFNEVN
                     IQTIGGNLLVQFSKESDQVFHNIWLCGPANFVFSGSISI"
     gene            3659..4504
                     /locus_tag="PLY81_02520"
     CDS             3659..4504
                     /locus_tag="PLY81_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF29134.1"
                     /translation="MPKEIAFLINGQSKETLQEQFIEERNQREAIIEKNTTDANTQQL
                     KPKIKTIVYFPGSFAVFFLILTIGICSKAIGQWMSFERTKQQIAHQKVNAELSYLKSQ
                     VNPHFFFNTLNNIYSLAVVQSDKTAASVLKLSSIMRYMLTEVQEEKTLLEKEIDHIKN
                     YIDLQMVRLTNKVSVSFTVNGTIDGIKVAPLLLIPFIENAFKYGVSTVEQSAIEIEIN
                     TTEKCLQFISSNTIVQTQNILQSSTGIGISNVKRRLELLYKDNYTLNITNNTFNFKVF
                     LEINI"
     gene            4507..5202
                     /locus_tag="PLY81_02525"
     CDS             4507..5202
                     /locus_tag="PLY81_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29135.1"
                     /translation="MISAIALDDEPLALQIIQQYCNKINFITLEKTFTNPNEAYLYLK
                     NNKINLLFLDIQMPDINGLQFFKSLPIKPSVIFTTAFKDYALEGFNVNAIDYLLKPFE
                     FERFLIAANKASTAIEGNNITTVQNFINVKVNYEIQKINLAEIELIEALDDYIKIYIK
                     PYPVLTLMTLKNMLEKLPADEFVRIHRSYIIPLNKVEKFNKHKVFVAGKEIPIGSSFS
                     SVYDILTQHNLQE"
     gene            5277..6140
                     /locus_tag="PLY81_02530"
     CDS             5277..6140
                     /locus_tag="PLY81_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29136.1"
                     /translation="MLNKHCTTVLFLLLFNYFASTAQNNFAIAINYTPVLNSNNFSTV
                     FGGVNGNQIKLDKQGLIREMEFIAYPKTVFNIVKTIPEKGYNIYEITTNDYPYSNKKL
                     YIDSRFVKLSKLKPTERVTKLPNQQDILSRMKKLEGFPYMWGGNTPNGVIEMLNLYKP
                     KGTINQTTKNLWILKGVDCSGLIYQATDGFTPRNTSSLVNYGNAVDNIENLNASQIAK
                     LLKPLDLIVWNGHVVVVYNETTTIESTPDGGVKTTDLTKRLQIILKDKKPVNNWNNTS
                     GKRFVVRRWIN"
     gene            6198..7721
                     /locus_tag="PLY81_02535"
     CDS             6198..7721
                     /locus_tag="PLY81_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF29137.1"
                     /translation="MNFLHELQLQNHNNGSSTGTQWLPSDGQLIKSFSPVDGNEIGTV
                     NSTTNNVYKSIVTKAEQAFEEWRLWPAPKRGEIVRQIGNALREKKEPLGKLVSYEMGK
                     SLQEGLGEVQEMIDICDFAVGLSRQLYGLTIHSERPNHRMYEQWHPLGIVGIISAFNF
                     PVAVWSWNAALAWVCGNVCIWKPSEKTPLCSIAVQNIVSEVFQKNNVPEGVNCLIQGY
                     KEAGEWLSQDAKVALVSATGSTRMGKAVNAAVAQRLGKTILELGGNNAIIISKDADID
                     MAIIGSVFGAVGTAGQRCTTTRRLIIHESIYDTLKQKLINAYQQLKIGNPLDSKNHVG
                     PLIDKDAVTQYLSSIEQCKQQGGKFIVEGGVLTGEGFESGCYVKPCIAEANNNMAIVQ
                     HETFAPILYIMPYHKIEEAIAMQNQVPQGLSSAIMTLNVREAELFLSHKGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWGSSLPLAQGIEFNI"
     gene            7837..8946
                     /gene="lpxB"
                     /locus_tag="PLY81_02540"
     CDS             7837..8946
                     /gene="lpxB"
                     /locus_tag="PLY81_02540"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF29138.1"
                     /translation="MKYFIIAGEASGDLHGSNLIKQLRLADDNAIIQCWGGNLMQNAG
                     ATLLKHYKELAFMGFIEVVKNLPTILKNIKFCKQQINSFKPDVVVLIDYPGFNLRIAE
                     WAKKQGYKIVYYISPQVWAWKAGRVKKMKQCIDKMICILPFEQTYYQKKWNWKVDYVG
                     HPLIEAINQFKANYTAPNNSSNKFIALLPGSRKQEIIKKLPIMLEVAKAFPDYKFVVA
                     KAPGQDDDFYNQFLPVQPNVEFFRNDTYNVLMQSKAALVTSGTATLETALFEVPEVVC
                     YKGSNISYQIAKRLINIKYISLVNLIMDKLVVTELIQNKLTVTNLTTELNSLLNNEQR
                     KQQLQKDYTELKQILSISQQPSALAAKIIVDFINL"
     gene            complement(8936..>9121)
                     /locus_tag="PLY81_02545"
     CDS             complement(8936..>9121)
                     /locus_tag="PLY81_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29139.1"
                     /translation="LDTKILNIKKKYLFIKKRDPNEPRTQWTKYMHGMNRISLFMFLI
                     ALLIITFKLFILPFFKG"
BASE COUNT         3404 a         1304 c         1456 g         2957 t
ORIGIN      
        1 aagcatatta gtgaaatgca aaagtactta agttctcagt ctaacataaa atctggtgca
       61 gtatggaaag gaagagaaaa tgtgttagat gcagcaatga aacaaagtga tagatggaga
      121 aatatgaaat cagaaggtgc cgatgaggag acaattaaaa aaagctttta tgaaaaaact
      181 aaaatgaaag ttttttcatg gaataaaaat agagaagcag atacagtaat gacaccattt
      241 gactctataa aatatagtag gcaaatgtta caagcaggtt ttatggtaat ggatccaatt
      301 actggagaag taaaagcctg ggttggtgga atagatttta aaaactataa atacgaccac
      361 gtaaacttta atactaaacg ccaggtaggc tctaccatta aaccattact ttatggtttg
      421 gcaatagaag aggcaggcct tacaccaaca tcaatggtac aagatgtaca acaaagtttt
      481 ggttcttatg gcttagtacc tgcaacaagt aaaacatgca caggtgctac aatgcctatg
      541 gcacaagcat tagctggttc aagaaactgt gcatctgctt atattataaa acagttagat
      601 aattctggca ataatggagc aagaaggttt gttgattttt taaaacgttg cgattttaaa
      661 actaaattag atgcttaccc tagtatggct ttaggtgctt gtgaaatttc gttatatgaa
      721 atgttacaag gctataccat gtttcctgga agaggcttta atgtacaacc catgtttatt
      781 acacgtattg aagatagaaa cggaaatgta ttagaaactt ttactccaaa gcgtaaagaa
      841 gttattagtg atgttacagc atactcagta ataaaaatga tggaaggagt aatggaattt
      901 ggaacaggta gaagaatttg gagttatgat gtaaaaggaa aaattgcagg taaaacagga
      961 accaccaatg acaatagtga tgcttggttt ataggatata cacctcaaat attagcaggt
     1021 acttgggttg gttgcgatga tagatttatt cgatttaatt caacagcaat agggcaaggt
     1081 gcttctgctg ctttaccaat ttgggcatat ttttatgaaa aagcatctag agaccctgca
     1141 tgtggtttag atgtaaccag cacttttgct aagccagagg cattaactaa tgatattatt
     1201 attgatggaa taagtggctt tacccctgta ccagggggtg aaacagaaga tggcggagca
     1261 ggcactagct ctgaatatgg ggaggaaagt agtgaatata atgatgactc taccacacct
     1321 actcaaacaa atgaaactca aaattcagac aataaaaata atcaagacaa caaaaataaa
     1381 acagaaaaaa aacaaccacc tgctacttta cccaataaag atcaaaaacc caaagctgtt
     1441 attccaaaga aaaatactta aaaccatttc ttctttaaaa aataccaacc aataactacc
     1501 gatattatta aagagagaat tacaggtata taaaatgcat gtgaagagtg ttggtacgga
     1561 atttcaacat tcattccata aatactggtt actaaaattg gtaacgataa aataatagta
     1621 atagaagtta aacgtttcat tacattattt aaattattgc ttataatact tgcaaatgca
     1681 tccattgtac tacttaaaat atttgcataa atatttgcca tctctaaggc ctggctggta
     1741 tctacaatca agtctctaag caattctcta tcatcttcat tcagccctaa aaaatttgtt
     1801 ctttctaatt tcatcattag catttcattg ctacgcaaag cagtaacaaa ataagtaagg
     1861 cttttttgaa tacgcattaa atttaataaa tcttcgttac tgttactatc gtataaactt
     1921 tgttcgtact ggtttctgcg attattaatt tgttttaaat agcctaaata atcttgtaca
     1981 atcttttcaa aaatttttaa taccatcata tttcttttat ctgtttgcct attttgaaaa
     2041 gtgtttaaaa actttttaat tgctccattg tcaaaagagt taacagtaac aatatggtta
     2101 tgggttaaaa tgatgcaaat aggtattgta atataaaagg catcgctatc attaaaagaa
     2161 ttattttcgg ctggagtttt aattactata aattttacat tgtcttcaag ttcatatcta
     2221 gatctttcgt caatatctaa agaatctctc aaaaactcaa taggaatatc taaattatta
     2281 cttaaatctg taaagtcttc ttctttaaaa ggtggcacta ggtttaccca aatatcattt
     2341 tctggcttac ttatttctac ggttttaccc tctatgtttt taaaatactg aatcataaat
     2401 aacccatttg tttttgttag aaataagtat taaggcaacg aaattacagt atgtttccta
     2461 aacaaaaatt tcaattttta agtagaagaa gttatataga aatggaacct gaaaaaacaa
     2521 aattggcagg accacatagc caaatattat gaaacacctg atcactttct tttgaaaatt
     2581 gaactaataa attacctcca atagtttgta tattaacttc attaaaccct actgtagttg
     2641 cattaactaa tgcagcagca gttacacctg taccacaact taatgtttca tcttctacac
     2701 ctctttcata agttcttact ttaatggtgt tatcattaat acgttgaaca aagtttacat
     2761 ttatcccttc tttttcaaac ctagagttgt atcttatttt tttaccctca gtatatacat
     2821 ctaaatctgt aatacttggc aaaattttta tgtaatgtgg agatcctgta tttaaaataa
     2881 aaacttcgtt atgttcattt tctacacctt taacatcttg aatttttaag tacacaatgt
     2941 ctttaacaac tgcttcgtgt aaaccatcta ttgcagtaaa taaatactta tcttttatta
     3001 aaccaatatc atgtgcaaat tgtgtaatac atcttcctcc attgccacac atagagcttt
     3061 cgtgcccatc tgcattatag taaatcattt caaagtcgta taggctatgg ctatttagta
     3121 acattaatcc atctgcacca atgccaaact ttctattaca taaaaaattt atttgttggt
     3181 aagataaatt tattttgttc tcacggttgt caataattat aaaatcattg cctgtgcctt
     3241 ggtatttaga aaaaaaaatg gtcattaata aaagaaatga atggttatta aaggtttttc
     3301 atacaaaagt aaataactat ttactataaa tttactacta tataaatcta aaaattaagc
     3361 tactttagct atctcaattt ttaataaatt gtttaaacaa aaaaaaatac taaatatcag
     3421 tattcatatt gcagtatgga tttgcttttt tctattacct tatatttttt ctcctcaacc
     3481 aaaagatatt tcagttcaag tttcaaaata tttagtggta ctatacattg ttataaatat
     3541 ttttttactt ctcttttatt actttaatac acttgtatta ataccaaaat tattatttta
     3601 aaaaagatgg tttatatact ctgtaattat ttccattttt tttattgcct atttgtatgt
     3661 gccaaaagaa attgcttttt taataaatgg tcaatctaag gaaacattgc aagagcaatt
     3721 tattgaagaa agaaatcaac gtgaagcaat tattgaaaaa aatactactg atgcaaatac
     3781 tcaacaactt aaacctaaaa ttaaaaccat tgtttatttt cctgggtcgt ttgcagtttt
     3841 ttttctaata cttaccattg gtatatgtag caaagctata ggacagtgga tgagttttga
     3901 gagaactaaa caacaaattg cacatcaaaa agtaaatgca gagttgtcct atcttaaatc
     3961 tcaggtaaac cctcactttt ttttcaatac tctaaataat atttactctt tagctgttgt
     4021 gcaaagcgat aaaacagcag catccgtttt aaagctttct tctataatga ggtatatgct
     4081 aaccgaagta caagaagaaa aaactttact tgagaaggaa atagaccata ttaaaaatta
     4141 tatagattta caaatggtga ggcttacaaa taaagtgtct gtaagtttta ctgtaaatgg
     4201 aacaattgat ggcataaaag ttgcaccact attattaatt ccttttattg aaaatgcttt
     4261 taaatatggt gtaagcacag ttgaacaatc tgcaattgaa attgaaataa ataccaccga
     4321 aaaatgttta caatttattt cttctaatac cattgtgcaa acacaaaata ttttacagtc
     4381 atctacagga attggaataa gtaatgtaaa aagaagatta gagttgttgt ataaagacaa
     4441 ttacacactt aacattacta ataatacttt taattttaaa gtttttctag aaataaatat
     4501 ataaaaatga ttagtgcaat agctttagat gatgagcctt tggctttaca aattattcaa
     4561 cagtattgta ataaaattaa ttttattact ctcgaaaaaa cttttactaa ccccaatgaa
     4621 gcatatttat atttaaaaaa taataaaatt aatttgttgt ttttagatat tcaaatgcct
     4681 gatataaatg gcttacaatt ttttaaaagt ttaccaatta agccttctgt aattttcacc
     4741 actgctttta aagattacgc tttagaaggg tttaacgtta atgcaattga ttacttatta
     4801 aagccttttg agtttgaaag atttttaata gctgccaata aagcaagcac agcaattgaa
     4861 ggaaataata taacaacagt gcaaaacttt attaatgtaa aagtaaacta cgagatacaa
     4921 aaaataaatt tagcagaaat agaattaatt gaggcattag atgattatat aaaaatttat
     4981 ataaaaccct atcctgtgtt aactttaatg actcttaaaa acatgcttga aaaattacca
     5041 gcagatgaat ttgttagaat acatcgttct tatataatac cacttaataa agtagaaaag
     5101 tttaataaac ataaagtatt tgtagcagga aaagaaatac caattggcag cagtttttct
     5161 tctgtttacg atatattaac acaacacaat ttacaggagt agtatttgtt tatatttttt
     5221 tacattttta tttcaatttt tactctcaaa tttgctttaa acgtaaacat aaatgtatgt
     5281 tgaacaaaca ctgcactaca gtattatttt tattactatt taattatttc gcctccactg
     5341 ctcaaaataa ttttgctatt gcaataaact atacacctgt attaaactcc aacaattttt
     5401 caacagtgtt tggaggcgta aatggcaacc aaataaaatt agacaagcaa ggtttaataa
     5461 gagaaatgga atttatagca tatcctaaaa ctgtgtttaa tattgtaaaa acaataccag
     5521 aaaagggtta caatatatat gaaataacca caaacgatta cccctacagc aataaaaaat
     5581 tatatattga tagccgtttt gtaaagcttt caaaactaaa gccaactgaa agagttacaa
     5641 aattgccaaa tcaacaagat atattaagta gaatgaagaa gttggaaggc tttccgtata
     5701 tgtggggagg caacacacct aatggggtta tagaaatgct taacctttac aagcccaaag
     5761 gcacaataaa tcaaaccacc aaaaatttat ggatattaaa aggtgttgat tgctcaggtt
     5821 taatttatca ggcaacagat ggctttactc caagaaatac ttcttcttta gttaattatg
     5881 gaaacgcagt tgacaatatt gaaaatttaa atgcttctca aattgctaaa cttttaaagc
     5941 ctttagattt aatagtttgg aatggccatg ttgtggtggt gtataacgaa actacaacta
     6001 tagaaagtac cccagatgga ggagtaaaaa caactgactt aacaaaaagg ttacagatta
     6061 ttttaaaaga taaaaagcct gtaaataatt ggaataatac atcaggaaaa agatttgtag
     6121 tgagaagatg gataaattaa taaaccaaat tttagatacc atacattcct ttactttgca
     6181 acattaaatt ttaggatatg aattttttac acgaattaca attacaaaat cataataatg
     6241 gcagttctac aggcacacaa tggctaccct ctgatggaca actcataaaa agcttttcac
     6301 cggtagatgg aaatgaaatt ggtacggtaa atagtaccac aaacaacgtg tataaaagta
     6361 ttgtaacaaa agcagaacaa gcttttgaag agtggcgttt gtggcctgcc ccaaaaagag
     6421 gagaaattgt aaggcaaata ggaaacgcac tgcgtgaaaa gaaagaacca ttgggtaaat
     6481 tagtgagcta cgaaatgggt aaaagcttac aagaaggttt aggcgaagta caggaaatga
     6541 ttgatatatg tgattttgct gtaggccttt ctcgtcagtt gtatggttta actattcata
     6601 gcgaaagacc taaccacaga atgtatgaac aatggcaccc attaggtatt gtaggtatta
     6661 tttcagcttt taattttcct gttgctgttt ggagttggaa tgctgcatta gcttgggttt
     6721 gtggtaatgt atgtatttgg aagcctagtg aaaaaacacc actttgttct attgcagtac
     6781 aaaatattgt gtcggaagtt tttcaaaaaa ataatgtacc agaaggagta aattgtttaa
     6841 tacaaggata caaagaagca ggagagtggc taagccagga tgctaaggtt gccttagtaa
     6901 gtgcaacagg tagtacaaga atgggaaaag ctgtaaatgc tgcagtagca caacgtttag
     6961 gaaaaactat tttagaatta ggtggcaata atgcaattat tatttctaaa gatgcagata
     7021 ttgatatggc aataattggt agtgtttttg gagctgttgg tactgcaggt caacgttgta
     7081 ctactacaag aaggttaata attcatgaaa gcatttatga tactcttaaa caaaaattaa
     7141 ttaatgcata tcagcaacta aaaattggta atcctttaga tagtaaaaat catgttggcc
     7201 cactaataga taaagatgca gttacacaat atttatcctc tattgaacaa tgcaaacaac
     7261 aaggtggtaa atttattgta gagggtggag tacttacagg agaagggttt gaatctggtt
     7321 gttacgttaa gccttgtatt gcagaagcca ataataatat ggccattgtt caacacgaaa
     7381 cttttgctcc tatattatat attatgccat atcataaaat agaagaggct attgcaatgc
     7441 aaaatcaagt accacaaggc ctatcctcag ctattatgac tttgaatgtg agagaggcag
     7501 aattattctt atctcataaa ggtagcgatt gtggtattgc aaacgtaaac attggcacaa
     7561 gtggtgcaga aattggtggt gcatttggag gagagaaaga aactggtgga ggaagagaaa
     7621 gtggtagcga tgcatggaaa gcatatatgc gtaggcaaac caatacaatt aattggggta
     7681 gcagtttacc attggcacaa ggtatagaat ttaatatcta aaccattaaa cattaagtac
     7741 aatcagccat cttaaattgt tgcatttatt ttggctgatt tttttatttc tgattctttc
     7801 aaaatatgaa ttacatcatt gtactttgca agtaatatga aatattttat aatagcagga
     7861 gaagcaagtg gagatttgca tggtagtaat ttaattaaac aattacgcct tgcagatgat
     7921 aatgctatta tacaatgttg gggcggaaac cttatgcaaa atgcaggtgc tactctttta
     7981 aaacattata aagagttggc ttttatgggc tttattgaag tagtaaaaaa cttgccaacc
     8041 atattaaaaa atataaaatt ttgtaaacag caaataaata gttttaagcc agatgtggta
     8101 gtattaatag actatcctgg ttttaattta agaattgcag aatgggcaaa aaaacaagga
     8161 tataaaattg tttattacat atctccacaa gtttgggcat ggaaggcagg acgagtgaaa
     8221 aaaatgaaac aatgcattga taaaatgatt tgcattttac catttgagca aacctactac
     8281 caaaaaaaat ggaattggaa ggtagattat gtagggcatc cattaattga agcaataaat
     8341 caatttaaag caaattatac agcacctaac aactcttcta ataaatttat tgcattatta
     8401 ccaggtagta gaaaacagga aattataaaa aaactaccca ttatgttaga agtggcaaaa
     8461 gcttttcctg attataaatt tgtagttgct aaagccccag ggcaagatga tgatttttac
     8521 aatcagttct tacctgttca accaaatgtt gaatttttta gaaacgatac ttataatgta
     8581 ttaatgcaaa gtaaagctgc attagtaaca agtggtacag caactttaga aactgcattg
     8641 tttgaagtgc cagaagtagt ttgttataaa ggaagtaata ttagctacca aatagcaaaa
     8701 aggctaatta atataaaata tatatcgttg gtaaatttaa taatggataa attagttgtt
     8761 accgaattaa ttcaaaataa attaacagtt acaaatctta ctaccgaatt aaatagttta
     8821 ctaaacaacg aacaaagaaa acagcaatta caaaaagatt acacagagtt aaagcaaatt
     8881 ttaagcatct ctcaacaacc ctctgcattg gctgcaaaaa ttattgtaga ttttattaac
     8941 ctttaaaaaa aggtaatata aacagtttaa aagtaattat cagtaaagca attaaaaaca
     9001 taaataaaga aattctgttc atgccgtgca tgtactttgt ccattgagtt ctaggctcat
     9061 tagggtctct ttttttaata aaaaggtatt ttttttttat atttaatatc tttgtatcta
     9121 a
//
LOCUS       DAOQHP010000043         9049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3905_length_9049_cov_3.814988, whole genome shotgun sequence.
ACCESSION   DAOQHP010000043 DAOQHP010000000
VERSION     DAOQHP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3905_length_9049_cov_3.814988"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1214)
                     /locus_tag="PLY81_02550"
     CDS             complement(168..1214)
                     /locus_tag="PLY81_02550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF29140.1"
                     /translation="MQQSILLIGLFLSEKNKHKIYRTAADQLAELLHKNNYSIIKAST
                     YVNKIPRFVHTIFSIIFNSSKYSVAIVPLYGGTFSLFWAEAATVFLKLFRKKIILIIH
                     GGSIPTRMENDRKAKNYLRTIQKAHVVVCPSNYIIHHLQKHGVNAVLIENVLNLSEYS
                     FHEKKLLRPTLFWMRTFEDVYNPLMAVRVLAILQNTYPSAKMIMAGRDAGLLEETKQL
                     AQKLNVIDKIVFPGYIDNTAKNNIAQEYDIYICTNSIDNAPVSTIEMMSLGLPVVSTN
                     VGGIPFIVTNNKDGLLVNLNDDEGMVSKIALLIENINIAEKIIQEGLNTSKKYDEEIV
                     IKKWNKIFKQLNFT"
     gene            complement(1214..1867)
                     /locus_tag="PLY81_02555"
     CDS             complement(1214..1867)
                     /locus_tag="PLY81_02555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF29141.1"
                     /translation="MSNNQLYHTNSSHLPQLALCHQKCFKGSLSEKLGLAYIAKTLDW
                     FLKDDNKFLFHFQNTEGQVIGYCGGFVSKKNGDGSSSGMLQHAFKEAVIGIVKKPWLI
                     FNKEVVAMYPFIFKNIKQKLFKKSKAKQVVDYSNEEKKFAGLVVIGVHPAYRGKGFFE
                     KLMEQFFIDASLKGAIGGALSVKKNNARGIAAYKKNGWIIVREDAHTYIMQKLTQTS"
     gene            complement(1839..3173)
                     /locus_tag="PLY81_02560"
     CDS             complement(1839..3173)
                     /locus_tag="PLY81_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF29142.1"
                     /translation="MIYKAIIWKFLLALGIGVIIGAKLINVQLFGFAYFGAVLLLAYF
                     AVFNKIEQLFTVLPFVIYSEIFVRDRLDSLVPYLTIQYLYIFIFSFMLIRFSGGNKPH
                     NKAFYFLLGFAFLEIFNGLNPDRPKLLRSIFFNSFSMILAVIWASHNVLTPKLINKIL
                     ANVKLAGMYLSGIVIVAQVTGGIDYGNFSNSESSNGLAPVQLSGYLSVAACFIFFSLM
                     NKDEIKSRYVYIIAFSIITGVMILTFSRGGLYFLGAILTLYLFFNRKNLGNYFKYLLF
                     LPIGLVIYNLAIEKTEGKIIARYEAKGTSNRDILVEAAFKLFLENPVVGVGTSNYGTA
                     IVEYRLFNQESTAHNEFARAMAEHGILGIITYWGFFISLFITIFKRKEPAKQFSVYFI
                     ALFIFITIHNGLKISIQHLLIIMAIANPTAVKAIFYRKIKPNTIVKQSAISY"
     gene            complement(3163..4326)
                     /locus_tag="PLY81_02565"
     CDS             complement(3163..4326)
                     /locus_tag="PLY81_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29143.1"
                     /translation="MNLGIVSDCVHFTTPTGVVATDNHIFLRQLNALAVHFDRVTICC
                     PFIEFDDSKSYSVYTDKKFHFIPLSNPGGNSIKHKLKLLLTIPEWFKAFSKINKASDI
                     VYQRFPNNLNIPGFFYFYFKRKKVFATYTGAWKNEINQSKTYQFQKWLLKKFFRGPVW
                     VYSSEEKLAKNIFGGFSPSYTIQEWNEETVQVNNRINFLSNNKLTSLKLISVGALNSN
                     KNQQYILNNCLLLKQATIPFSLHIVGEGVLKNEYQNFIDQHNLNNEIILTGALNYTAL
                     RNLYREKDFVVQATLSEGFGKVPIEGFFHGLVPILHQSVMAPYFTNNGTRGYLFNAEN
                     SNDLFHVLSNVYSTTSAKLIAEKIKEGRIFVQSQTLENWASIYFSTVKKYFDL"
     gene            complement(4349..5698)
                     /locus_tag="PLY81_02570"
     CDS             complement(4349..5698)
                     /locus_tag="PLY81_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNF29144.1"
                     /translation="MIFSFIKYTQPAWYFNVLPSVKEFSCCYYHPKWLPLPNGILLDE
                     DTAYETEAAINADIGYRAWHSGYLIKACNNDLQKIIQLPKPSINDEYRFVTKYWGMHW
                     AALTLVRRIFTLHNPIKEIKGFIKNVNTPRYNLYNTVADWSEYKNFQSVLVEQQPLVS
                     VIIPTLNRYEYLADVMLDLEKQTWKNFEVIVIDQSENFNDAFYKKYNLKINVVQQKEK
                     LLWTARNNAVKLSKANYLLFFDDDSRVEPDWIEQHIKTLDFFNASISAGVSIAVVGGK
                     VSKSYDHFRWADQFDSGNALVKREVFEKIGLFDLQFNKERMGDSEFGIRAYLNGYKSI
                     SNPYGKRVHLKVSSGGLREIGHWDAFRPKKLFAPKPVPSVIYLFKKYYPSYLSKNVIY
                     LGIMLSNISYKSKSKNSMLALSVLLTFLKFPLLYIQYKKSLALANKKLQQGDKIEKL"
     gene            complement(5708..6883)
                     /locus_tag="PLY81_02575"
     CDS             complement(5708..6883)
                     /locus_tag="PLY81_02575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF29145.1"
                     /translation="MNIAIVVNNFPSNSETFIINKVIELAKAKNSVTVFSLNGTGNKD
                     LYELYHFKNLPNISIKYINLPKNIFELIKFFLSNPLLTISSFSATPKIFFSNIKSTLL
                     TKHFNNKFDIVHFEYSGLGASLLEIIPLVKSKKVVSCRGSAEKVKPLTDATRKEKLQQ
                     LFNLVDGIHCVSEDMKQTITPYCNNIEKVFVNRPAIDATFFQPPAVKPQNNITQILTV
                     GRFTFQKGYLLLLMVAKKLATKNINFVWNIIGEGPQHEELVFHIHTLGLEKCVKLVGK
                     KNKNEVNQYLASANMFVLTSVYEGIPNVVLEAMAMELPVISTNCGGVEEVIETGIDGF
                     IAPLYDVETIANQIEYLIAKPDKANEMGRLARKKILESFTLERQVNIFVEAYNKIIG"
     gene            complement(6889..8736)
                     /gene="asnB"
                     /locus_tag="PLY81_02580"
     CDS             complement(6889..8736)
                     /gene="asnB"
                     /locus_tag="PLY81_02580"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNF29146.1"
                     /translation="MCGIVGVFSYKCRTEKYNTYLSKCCKSMKHRGPDDSGLWYNNEN
                     YSAAFVRLSIRDLSLNGHQPMISACGNFVITFNGEIYNSNNYVAELKKLGVHFKSNSD
                     TEVLLYALIYFGIEKVLAEFDGIFAFSFYDVQKNKLFIARDRVGTKPLYIGFNNDFII
                     YSSQYDHIINIDFIKNNSLDYQSLGNYLSFGYILAGEGVIKKTFSLPQGHYLETTNDS
                     FLIHKYYEFDEKKNISISNVEALFEEATISQMVSDVPLGTFLSGGVDSPLINFWANKK
                     QQVKAFTIGNETDELDESYYAKAFANKIGVEHHFKKITENDFLQLLEDNFKAFSEPFA
                     DFSSIPTMLVSKMAKEHLTVILSGDGPDELFWGYNRNVIFPNKATLFNQGKLSLAVNK
                     ILGKGNISKRYFTADSIAHFYLQSLQIHGAEASLPKVYLEKIVNNKIFSLLPERLQQP
                     KNMNDIMQMVRWLEMNIHLQRILLKVDRASMFYSLEARVPYLSNAVLDYASTLNYSDC
                     INENQGKVNMKRILETKVDKEWVYKKKKGFDIPMRQWIQNEIKQDVYETILNMPTELA
                     CVFEIDNLENILQNEYDASNKNSNYGFIWAVYSLVKWHQNHRNSSILKD"
     gene            complement(8746..>9049)
                     /locus_tag="PLY81_02585"
     CDS             complement(8746..>9049)
                     /locus_tag="PLY81_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29147.1"
                     /translation="VVKNLIKVTTKGGYILLTVPYKYKKHLDPIDNMFRPTPNEIISL
                     FKDVATEVIDKQVIVIHDKSYYHKKKSKFPIWGNREIIAYHLGIRHKVSGVLLQLK"
BASE COUNT         3100 a         1486 c         1142 g         3321 t
ORIGIN      
        1 ttcttcaaaa ttggtataat agggcacatt atatttttct gctaatgctt ttgctttttc
       61 ttctactgta tcgcatactg cttttaatat accaagttta ttaatatgct ctgcatgcct
      121 ttgtgcaatt cttccacatc caattaatgc aaattttatt ttatccatta tgtaaaattc
      181 agttgtttaa aaattttatt ccattttttt atcacaattt cttcatcgta ttttttagaa
      241 gtatttaagc cttcttgaat tatcttttct gcaatattta tattctctat tagtaatgca
      301 attttactta ccattccttc gtcatcattc aagttcacta ataaaccatc tttattatta
      361 gttactataa aaggaatacc tccaacattt gtacttacaa caggtaagcc taaactcatc
      421 atttcaatag tgcttactgg tgcattatca atgctattgg tgcaaatata aatatcatac
      481 tcttgggcta tattattttt tgctgtatta tctatataac ctggaaaaac tatcttatct
      541 ataacgttta atttttgtgc taattgcttt gtctcttcta aaagccctgc atctctgcct
      601 gccattatca tttttgcaga tgggtatgta ttttgtaaga tagccaaaac tcttacagcc
      661 attagcggat tatacacatc ttcaaatgtt ctcatccaaa ataatgttgg ccttaataat
      721 tttttttcat gaaaagaata ttcagacaag tttaaaacat tttctattaa aacagcatta
      781 acaccatgct tttgtaaatg atgaatgatg tagttagaag ggcacaccac aacatgtgct
      841 ttttggattg ttcttaaata attttttgct tttcgatcat tttccattct agttggtatg
      901 gaacctccat gaatgattaa gattattttt ttacggaaaa gttttaagaa aactgttgct
      961 gcctctgccc aaaacaaact aaatgtgcca ccatacaatg gcactattgc aacagaatat
     1021 ttggaagaat taaaaataat agaaaatata gtatgtacaa aacgaggtat tttatttaca
     1081 taagtggatg cttttataat agaataatta ttcttatgta aaagttctgc taattgatct
     1141 gcggcagttc tataaatttt atgtttattc ttttcagaaa gaaataaacc aattagtaat
     1201 atactttgct gcattaactt gtttgggtta atttttgcat gatgtaagta tgtgcatctt
     1261 ctctaacaat tatccatcca ttttttttat aagctgcaat acctcttgca ttattttttt
     1321 taacactaag agcaccacca atagctcctt ttaaagaagc atcaataaaa aattgctcca
     1381 ttagcttttc aaaaaaacct ttacctctat aagcaggatg tacaccaata acaaccaagc
     1441 cagcaaattt tttttcttca tttgaataat caacaacctg ctttgcctta ctttttttaa
     1501 aaagtttttg ttttatattc ttaaaaataa atgggtacat tgctacaact tctttattga
     1561 agataagcca aggttttttt acaataccta ttacagcctc tttaaaagca tgttgcaaca
     1621 taccactgct acttccatct ccattttttt tagaaacaaa accaccacag taacctatta
     1681 cttgcccctc ggtattttga aaatgaaata aaaacttatt atcatctttt aaaaaccagt
     1741 ctaaggtttt agcaatataa gctaagccta atttttctga caaagagcct ttaaaacatt
     1801 tttgatgaca taaagccaat tgtggtaaat ggctgctatt agtatgatat agctgattgt
     1861 ttgacaatag tattaggttt tatttttctg taaaaaatag ctttaacagc agttggatta
     1921 gcaattgcca taatgattaa taaatgctga atgcttattt ttaaaccatt gtgaatggtg
     1981 atgaaaataa ataaagcaat aaaataaaca gaaaattgtt tggcaggttc ttttcgttta
     2041 aaaattgtaa taaataatga aataaaaaat ccccaataag taattattcc taaaatgcca
     2101 tgctctgcca tagctcttgc aaattcatta tgtgctgtac tttcttgatt aaacaatctg
     2161 tactccacaa ttgcagtacc atagttactt gtacctacac ctactactgg gttttctaaa
     2221 aatagtttaa atgcagcttc tactaatata tctctgttag atgtgccttt agcttcgtac
     2281 ctagcaataa ttttaccttc tgttttttca atggctaaat tataaataac taaaccaatt
     2341 ggtagaaaca ataaatattt gaaatagtta cctaaatttt ttctattaaa aaataaataa
     2401 agtgtaagaa ttgctcctaa aaagtataag ccaccacgag aaaaagttaa aatcattaca
     2461 cctgtaataa tggaaaatgc tataatataa acatacctgc tttttatttc atctttattc
     2521 attaaagaaa agaaaataaa acaagcagcc acacttaaat atccacttaa ctgtacaggt
     2581 gctaaaccat tacttgattc tgaattggaa aagtttccat aatctatacc acctgtaact
     2641 tgtgcaacaa ttacaatacc agataaatac ataccagcta attttacatt ggctaaaatt
     2701 ttattaatta gctttggtgt tagcacatta tgagatgccc aaataactgc taaaatcatt
     2761 gaaaaactat tgaagaaaat agaacgtaat agttttggcc tatctgggtt taggccattg
     2821 aatatttcta aaaatgcaaa gcctaataaa aaataaaatg ctttattgtg tggtttattt
     2881 ccaccactaa acctaataag catgaaggaa aaaataaaaa tatataagta ttgaattgtt
     2941 aaatatggca ctaaactatc taacctgtct cgtacaaata tttcgctata aattacaaaa
     3001 ggaagtacag taaacagttg ttctatttta ttaaaaacag caaaataggc aagcagtaaa
     3061 acagcaccaa aataagcaaa accaaataac tgaacattaa ttagttttgc cccaataatc
     3121 actcctattc caagtgctaa taaaaatttc catataatgg ctttataaat caaaatattt
     3181 ttttactgtt gaaaaataaa tagacgccca attttctaag gtttgagatt gaacaaatat
     3241 ccttccttct ttaatttttt cggctattaa ttttgcagaa gttgtggaat aaacattact
     3301 taatacatgg aacaaatcat ttgaattttc cgcattaaat aagtagcctc tagtaccatt
     3361 attggtaaaa tagggagcca taacagattg atgcaatatg ggaactaagc catgaaaaaa
     3421 accttcaatg ggcactttac caaacccttc tgacaacgtt gcttgtacca caaaatcttt
     3481 ttctctatat aaatttctta atgctgtata atttaaagca cctgttaaaa taatttcatt
     3541 gtttaggttg tgttggtcaa taaaattttg atactcattt ttcaataccc cttcaccaac
     3601 aatatgtaaa ctaaagggta tggtagcttg ttttagcaac aaacaattgt ttaaaatata
     3661 ttgttggttt ttatttgaat taagtgcccc tacagaaatt agttttaagg atgtaagttt
     3721 attgttactt aaaaaattta ttctattatt tacttgcact gtttcttcat tccattcttg
     3781 tatagtgtaa gatgggctaa agccgccaaa tatattttta gctaactttt cttcagatga
     3841 ataaacccaa accggccctc taaaaaattt ctttaacaac catttttgaa attggtatgt
     3901 tttagattga ttaatttcat ttttccatgc accagtataa gtagcaaaaa ctttttttct
     3961 tttaaaataa aaataaaaga agcctggtat attaagatta ttaggaaatc tttgataaac
     4021 aatatcgctt gctttattta ttttactaaa tgctttaaac cactctggaa tggtaagtaa
     4081 caattttaat ttgtgtttta tgctattacc acctggattg gaaagaggaa taaagtggaa
     4141 ttttttatcg gtataaaccg aataggattt gctatcatca aattcaataa aagggcagca
     4201 aatagttact ctatcaaagt gaacagctaa ggcattaagt tgacgaagga atatatgatt
     4261 atctgttgca acaacacctg ttggtgtagt aaaatgaaca caatccgaaa caattcctaa
     4321 attcattaaa gtagtgactt aaattaaact ataatttttc aatcttatcg ccttgttgca
     4381 actttttatt agctagtgct aaagattttt tgtattgaat gtataataat ggaaatttta
     4441 aaaaggtaag caatacactt aatgcaagca tactattttt acttttactt ttatacgaaa
     4501 tattggaaag catgatacct aaataaatta cattttttga caaataactt gggtaatatt
     4561 ttttaaataa atagataaca cttggcactg gtttgggtgc aaataatttt ttaggtctaa
     4621 aggcatccca atgtccaatt tctctcaatc caccactact tacttttaag tgaactcttt
     4681 tgccataagg gtttgagatg cttttatacc cattcaaata agctctaatg ccaaactcac
     4741 tatctcccat tctctcttta ttaaactgca aatcaaacaa accaattttt tcaaatactt
     4801 ctcttttaac taacgcattg ccactatcaa actgatcggc ccatcgaaaa tgatcatagc
     4861 tttttgaaac tttgccacca actactgcaa tggaaactcc tgcagaaata gatgcattaa
     4921 aaaaatctaa ggttttaata tgttgttcaa tccaatctgg ttctactctg gaatcatcat
     4981 caaaaaataa taaataattt gctttgctta gtttaacagc attgtttctg gcagtccaca
     5041 ataatttttc tttttgttga acaacattaa ttttgagatt atattttttg tagaaagcat
     5101 cgttaaaatt ttctgattgg tctatcacaa ttacttcgaa attcttccaa gtttgctttt
     5161 ctaaatcaag cattacatct gctaaatatt cgtatctatt taaagttgga ataatgacag
     5221 agacaagagg ctgctgctct actaaaacag attggaaatt tttgtattca ctccaatcag
     5281 caacagtatt gtataaatta tatcttggag tgtttacatt ttttataaat ccttttattt
     5341 ctttaatagg attatgcaag gtaaaaatcc ttcttactaa tgtaagtgca gcccaatgca
     5401 taccccagta ctttgtaaca aacctatact catcattgat gcttggttta ggcaactgaa
     5461 taattttttg taaatcattg ttgcatgctt taattaaata tccactatgc catgctctat
     5521 aaccaatatc tgcatttatt gctgcttcag tttcgtaagc agtgtcttca tccaacaaaa
     5581 tgccatttgg taaaggcaac catttaggat gataataaca acaagaaaat tcctttacac
     5641 ttggcagaac attaaagtac catgctggtt gagtatattt tataaaagag aaaatcacag
     5701 taaattctta accgattatt ttattatagg cttctacaaa aatatttact tgcctttcta
     5761 aagtaaatga ttctaaaatt ttctttctgg ctaacctacc catttcatta gctttatctg
     5821 gtttagcaat caaatattct atttgattag caattgtttc tacatcatac aaaggtgcta
     5881 taaatccatc tattcctgtt tcaattactt cttctacacc accacagttg gtagatatta
     5941 caggcaattc cattgccatt gcttctaata ctacattggg tattccttca taaacacttg
     6001 taagtacaaa catatttgca gatgctaaat attggtttac ttcattctta tttttctttc
     6061 ctactaattt cacacatttt tctaaaccta aagtatgtat atgaaaaaca agttcttcat
     6121 gctgtggtcc ttcgccaata atattccata caaaatttat gtttttagtt gctaattttt
     6181 tagcaaccat taataacaat aaataccctt tttgaaaagt gaatctgcct actgtaagta
     6241 tttgtgtaat gttgttttgt ggctttacag caggtggttg aaaaaatgta gcatcaattg
     6301 caggcctgtt tacaaaaacc ttttcaatat tattgcaata aggtgtaatg gtttgtttca
     6361 tatcctcact aacacaatgt attccatcaa ccaaattaaa tagttgttgt aacttttctt
     6421 ttctagtagc gtctgttaaa ggctttactt tttctgcact acctctgcaa ctcactactt
     6481 ttttagattt tactaaagga ataatttcta gtaaagaagc tcctaaacct gaatattcaa
     6541 aatgaactat atcaaactta ttattaaaat gtttggtgag taatgtagat tttatgttgc
     6601 tgaaaaatat ttttggagtg gctgaaaaag aactaatggt aagtaaaggg ttacttaaaa
     6661 aaaacttaat taattcaaaa atatttttag gtagatttat atacttaatt gaaatgttgg
     6721 gtaaattttt gaaatggtat aattcatata aatccttatt gccagtgcca tttaaactaa
     6781 atactgttac tgaattttta gctttagcca attcaattac tttgttaata ataaaggttt
     6841 cgctgttgga tggaaaatta ttaacaacga tggcaatatt cattttattt aatcctttaa
     6901 aattgatgaa ttacgatgat tttgatgcca ttttactaag gaataaacag cccaaataaa
     6961 tccataatta gaatttttgt ttgaggcatc atattcattt tgtaaaatat tttctagatt
     7021 atctatctca aaaacacaag ccaattctgt tggcatattt aatatagttt catatacatc
     7081 ttgtttaatt tcattttgaa tccattgtct cattggtata tcaaaccctt ttttcttttt
     7141 ataaacccat tccttatcca cttttgtttc taaaattctc ttcatgttaa ccttaccctg
     7201 attttcatta atacaatctg aataattaag tgttgatgca taatctaata cagcattaga
     7261 aaggtaaggt actcttgctt ctaaactata aaacatacta gctctatcta cttttaaaag
     7321 tatcctttgc aaatgaatat tcatttctag ccatctaacc atttgcataa tatcgttcat
     7381 attttttggt tgttgcaaac gttctggcaa taaagaaaat attttattgt ttacaatttt
     7441 ttctagatac acttttggta aagaagcctc tgccccatga atttgcaaag actgtaaata
     7501 aaaatgtgca atagaatcgg cagtaaaata tcttttagaa atgttaccct tccctaaaat
     7561 tttatttaca gccaacgata attttccctg attaaaaagt gttgctttat ttggaaaaat
     7621 tacgttacgg ttgtatcccc aaaataattc atctggacca tctccactta aaataacagt
     7681 taaatgttct tttgccattt tagaaaccaa catagttgga atagaagaga aatcggcaaa
     7741 tggttctgag aaagctttaa aattatcctc caataattgt agaaaatcgt tttcagtaat
     7801 ttttttgaaa tgatgctcca caccaatttt atttgcaaag gctttagcat agtagctttc
     7861 atctaattca tctgtttcat ttcctatggt gaaagctttt acctgttgct ttttatttgc
     7921 ccaaaaatta attaaagggc tatctacccc accagataaa aatgtgccta aaggaacatc
     7981 acttaccatt tgagaaatag ttgcttcttc aaataatgcc tctacattgc ttatagaaat
     8041 attctttttt tcatcaaatt cataatactt atgaattaaa aaactatcgt ttgttgtttc
     8101 taaataatgg ccttgcggta aagaaaaagt ttttttaata acaccctcgc cagcaagtat
     8161 ataaccaaat gaaagataat tgcctagtga ttgataatct aaactattat tttttataaa
     8221 atcaatatta attatatgat catactgaga gctgtatatt ataaaatcat tgttaaagcc
     8281 aatgtacaaa ggttttgtac caactctatc cctagcaata aataatttat ttttttgaac
     8341 atcataaaaa gaaaaagcaa aaattccatc aaactctgct aaaacttttt ctataccaaa
     8401 atatattaag gcatataaaa gcacttcagt atcgctatta gatttaaaat gtacacctaa
     8461 ttttttaagt tctgcaacat aattattgct gttataaatt tctccattaa aagtaatcac
     8521 aaaattacca caagccgaaa tcattggctg atgcccgttt aacgataagt ctctaataga
     8581 aagcctaaca aaagcagcag aataattttc gttattatac cataatccgc tatcatctgg
     8641 ccctctatgt ttcatagatt tacaacactt gcttagataa gtattgtatt tttctgttct
     8701 acacttataa ctaaaaactc ctacaattcc acacatacta gaaagttatt ttaattgtaa
     8761 taacacaccg cttactttgt gtcttatacc taaatggtat gcaataattt ctctgttacc
     8821 ccaaatagga aatttagatt tctttttatg ataataactt ttatcatgaa ttactatcac
     8881 ttgtttatct attacttctg ttgcaacatc tttaaataat gaaattattt catttggtgt
     8941 tggcctaaac atattatcta taggatctaa atgtttttta tacttataag gcacagtaag
     9001 taatatataa ccacctttag tagttacttt tattaaattt tttacaacc
//
LOCUS       DAOQHP010000044         8970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_3948_length_8970_cov_3.557824, whole genome shotgun sequence.
ACCESSION   DAOQHP010000044 DAOQHP010000000
VERSION     DAOQHP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_8970_cov_3.557824"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="metG"
                     /locus_tag="PLY81_02590"
     CDS             <1..1177
                     /gene="metG"
                     /locus_tag="PLY81_02590"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNF29148.1"
                     /translation="ILPDNVPSNEFMNLEGDKMSTSRGWSIEMEDYINGWIKKENGGE
                     SMADVLRYYLTNIAPETKDSEFTWKGFQDANNSELVSIFGNYVNRTFVLMHKLCGGKV
                     PAFHNNLLDETDKQLIIDIAQTKNSVEQLLEQYKFREAQFEVIDLARKANQYMQKKEP
                     WIVAKTLNETPNNQQLIDNCMHLCLQLTANLAILINPFLPFTAKKMLYMMKVVDKMLE
                     WKNAGSVKLLSVGYSLREPQLLFRKIEAEEINEQLEKLKQKAMLGKTEVATSEEVNVE
                     AELKNFKPEIQFDDFTKIDLRIGTIKTAEKVAKADKLLKLEVDLGSEVRTIVSGIAMH
                     FTPEEIINKQVTVVCNLAPRKMRGIESNGMILMAEDNNGKLHFINPDNVIKEGSSVS"
     gene            complement(1182..2414)
                     /locus_tag="PLY81_02595"
     CDS             complement(1182..2414)
                     /locus_tag="PLY81_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF29149.1"
                     /translation="MNLNVPFFIAKRLAINKKQSFSAFIIKLSIAATALSVAAMIITS
                     AFVNGFQDTVAKKVFSFWGHLRVQYYETSKAFISEENPIPKSMEVENILKKNNQVKHI
                     QPFATKSGVLEKNRNIEGILFKGIDETYDSSTIKPFILQGNWLNFKDSLYSKDIMISS
                     ILANELNIKLYDTVNVFFVSANEGESIYRPVIVKCIYKTGIEEYDKIFVVGDIKLIRR
                     LSNWNEDEIGGYEVILKDYQQMNAVNNNLDLPVIWNSKTIQQIYPNIFEWLQIQDLNK
                     NVMFIIMSIVAIINLITCLLILVLERIKMIGILKAVGYSNSSIQKIFLYHASIIAFIG
                     IIIGCFVGIGLCLLQQYTSFITLDESNYYVSVAPVKIIWGQVFAICGAVLVICFASLS
                     LPVILVKKINPLQAIQFR"
     gene            2577..3857
                     /locus_tag="PLY81_02600"
     CDS             2577..3857
                     /locus_tag="PLY81_02600"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNF29150.1"
                     /translation="MKKPFLMLFFLITFYAVLAQEKFSDAIYYPEIKTVQLYQQNNQM
                     SLPIINLGSSDLLELHFDDLDGYVKNYSYTFQLCNENWEPVDLSPFDYIKGFIQNRLN
                     QYRVSSIAQTKYVHYQALLPDRGSVPTKSGNYLLKVFLNGDINKVAFTKKFFVLDNKA
                     SIGLQVLQPYDNSLFRTYQRVQVSVNATQLNPINPQQQVKVVVLQNNRWDNVVRNIKP
                     AFIRGNVLEYNGEQDCLFAAGKEYRWVDLRSFRIATERVHSIDKLTIPNEIILKPDLT
                     RSQLRYAYFKDFNGWYFISSSENINPFWQADYANVHFTFIPPNNKAIEGKDVYIMSNF
                     TNSELNDSTKLLYNEEEGWYEKTFLMKQGYYSYTYVTKDKNNENAIADVTLTDGNLWE
                     TENDYTVFVYYKSVNGRHDELVGITTVNTRLLNR"
     gene            complement(3860..4891)
                     /locus_tag="PLY81_02605"
     CDS             complement(3860..4891)
                     /locus_tag="PLY81_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HNF29151.1"
                     /translation="MKNNFSYILLLIVVSIFIASIVSNFKGDDWEENSRKALYFLKGD
                     SVPSTATEYTLSNGVPYVHYVAENGVPAITRFNSTIVANYAIKYAQQFLENSDSTYYK
                     KFLNCVQFLKDSVTQQNNLTFYQFNWQQGWYPNISPPFISSMTNGRAIEVFLYAYQFT
                     NNNYYLQFAKNLLHAFYITVQNNGFAYKLNDSAWWYEEIAKPNVTTPYILDGHIYAIQ
                     GIQKLWLTTKSDSALYLFEKGINALKLKLPSYNAGKGEIYYDAHQHKADKKYHKILTN
                     QIYQLWQSTNDSTFLRYYQQWIKPSKVPYVIRMLKEKNISGLLLIAMLSALFFPILYL
                     VTNKIVLKK"
     gene            complement(4961..6772)
                     /gene="typA"
                     /locus_tag="PLY81_02610"
     CDS             complement(4961..6772)
                     /gene="typA"
                     /locus_tag="PLY81_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNF29152.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKILHATKIFRENQDSGELIMDNN
                     DLERERGITIFSKNAAVKYNGVKINVIDTPGHSDFGGEVERVLKMADGVILLVDAFEG
                     PMPQTRFVLQKALQLNLHPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLNFPT
                     FYGSGKNGWFNDSLTPTDNILPLMDGILKYVPAPKVAEGALQLQITSLDYSSFLGRIA
                     IGKVTRGSIKENMPIALVQADGTIKKSRVKELYVFEGMGKSRVNEVKAGDLCAVVGLE
                     DFNIGDTIADAENPEALPIISVDEPTMSMTFSINNSPFFGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDTDSADSFLVYGRGILHLGVLVETMRREGYELTVGNPQVIVKQINGKK
                     HEPYEVLVVDVPSEYSGKVIDLVTQRKGEMLVMESKGEMQHLEFDIPSRGLIGLRSQM
                     LTGTAGEAVMAHRFSEYKPWKGAIPGRNNGVLISKFQGATTAYSIDKLQDRGRFFVDP
                     GEEVYIGQVMAEHIKPGDLNVNAIEGKKLTNHRASGSDEGIRITPKILMTLEECMEYI
                     QQDECIEVTPKNIRMRKVILDENERNKAAKSLKVEAV"
     gene            complement(6966..8810)
                     /locus_tag="PLY81_02615"
     CDS             complement(6966..8810)
                     /locus_tag="PLY81_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29153.1"
                     /translation="MKHLAALNKYFWKYKKLFIAGIFCIIVSNYFRILTPQITGYIVD
                     AVQEKLATNQQIHTVAPKQYNYNYLVLMLINWLTTKQFSLSEIVVLCSVSLLVFAILG
                     GLFMFLMRQTIIVMSRHIEFAQKNEVFKKYLSLDTSFFKAHATGDLMNRISEDVSRVR
                     MYSGPAVMYIINLAFTIGFSIFFMLKANIELTLYVLSPLPFLAFIIYVVNSVINKKSE
                     KIQEQLSNLTSSAQEAYSGIRVLKSFVQENAITNFFSKKSNEYKQSAISLAKLEAFYF
                     PSMALIIGISTLLTIAIGSWYVIHKNNGVTAGTITEFVIYINMLTFPVSAIGWTASII
                     QRAAASQKRINNFLEIEPEIKNNSNKTEIDISGDIVFNNVSFIYQNTGIEAIKNFNLT
                     INKGSKVLVLGKTGSGKSTLAQLLLRFHNPKTGSITIGNNNLNSLNLYKLREQISYVP
                     QDVFLFSDSIKNNINFGLSNEAEMELIQNVAKLASVHNDILEFENGYETIIGERGVTL
                     SGGQKQRISIARALLKSPEIIVFDDCLNAVDAKTENTIIQNFYTFLQNKTAIIITHRI
                     FSSFKFDKIIVLNEGKIIEAGTHETLMALNGYYAALYKKQLTEEENNL"
BASE COUNT         3170 a         1340 c         1342 g         3118 t
ORIGIN      
        1 tattttgcca gataatgtgc cgagcaatga gtttatgaat ttagaaggag ataaaatgag
       61 cacaagcaga ggatggagta ttgaaatgga agattatata aacggttgga taaaaaaaga
      121 aaatggtggc gaaagtatgg cagatgtatt gcgttattat ttaacaaaca ttgcaccaga
      181 aacgaaagat agtgagttta catggaaagg ctttcaagat gctaataata gcgaattagt
      241 tagcattttt ggaaattacg taaacagaac ttttgtgtta atgcacaaat tatgcggagg
      301 taaagtacct gcttttcata ataacttact tgatgaaaca gataaacaac tgataattga
      361 tattgcacaa acaaaaaatt ctgttgaaca attattagaa caatataaat ttagagaagc
      421 acaatttgaa gttattgatt tagcaagaaa agcaaatcaa tatatgcaga aaaaagaacc
      481 ttggattgtt gctaaaactt taaatgaaac acccaacaat cagcaattaa ttgataattg
      541 tatgcattta tgtttacaat taactgctaa ccttgctatt ttaattaatc catttttacc
      601 atttactgct aaaaaaatgt tgtatatgat gaaggtggtt gataaaatgc tggaatggaa
      661 aaatgcagga agtgtaaaac tattaagtgt agggtatagt ttaagagaac ctcaattatt
      721 attcagaaaa attgaagccg aagaaattaa tgaacaatta gaaaaattaa aacaaaaagc
      781 aatgttgggt aaaacggaag ttgctactag cgaagaagta aatgttgaag ctgaattaaa
      841 aaattttaaa ccagaaatac agtttgatga ttttacaaaa attgacttaa gaattggtac
      901 tattaaaact gctgaaaaag tagcaaaagc tgataaactt ttaaaactgg aagttgattt
      961 aggaagtgaa gttagaacta ttgtttctgg cattgccatg cactttacac cagaagaaat
     1021 tattaataag caagtaactg ttgtttgtaa tttagcacct agaaaaatgc gtggtataga
     1081 aagtaatgga atgattttaa tggcagaaga taataatggc aaactacatt ttattaatcc
     1141 agataatgtt attaaagaag gcagtagtgt aagttaaaac cttatctaaa ctgtattgct
     1201 tgtaatgggt taattttttt aactagtatt acaggtaatg atagagaagc aaaacaaata
     1261 accagtaccg ccccacaaat tgcaaatact tgcccccaaa taatttttac aggagcaaca
     1321 gatacataat aattactttc atctaaggtt ataaaactag tatattgttg taataggcaa
     1381 aggccaatgc caacaaaaca gcctattatt attcctatga aagcaattat acttgcatga
     1441 tatagaaata ttttttgaat agagctattg ctatatccaa cagcttttaa aattccaatc
     1501 atttttattc tttcaagcac taaaatgagt aaacaggtaa taagattaat gatagccaca
     1561 atactcatta taatgaacat tacatttttg tttaaatcct gaatttgaag ccattcaaaa
     1621 atattagggt aaatttgttg aatagtttta ctattccaaa taacaggcaa atctaggttg
     1681 ttgtttacag cattcatttg ctgataatct tttaaaataa cttcatatcc gccaatttca
     1741 tcttcattcc agttgcttaa tctgcgtatt aatttaatat cgccaacaac aaaaatttta
     1801 tcgtattctt caattcctgt tttgtaaata cattttacaa ttacaggtct gtaaatagat
     1861 tctccctcgt tagcagatac aaaaaataca tttacagtgt cgtacagttt tatgtttagt
     1921 tcattggcca ggatactaga tatcataata tccttactgt ataaactatc cttaaaattc
     1981 agccaattgc cttgtagtat aaatggttta atagtactgc tatcataagt ttcatcaata
     2041 cctttaaata aaataccttc aatatttcta tttttttcca atacaccact tttggtagca
     2101 aagggttgaa tatgctttac ttgattattc ttctttaaaa tattctctac ctccatactt
     2161 tttggtatag gattttcttc ggaaataaaa gctttgcttg tttcgtaata ttgtactctc
     2221 aagtgacccc aaaagctaaa tacttttttt gcgactgtat cttgaaaacc attaacaaaa
     2281 gcagatgtta taatcattgc agccacactt aaagcagtag cagcaataga tagtttaatg
     2341 ataaaagcag aaaaagattg ctttttatta atagctaaac gctttgctat aaaaaaaggt
     2401 acattcaaat tcaaaaatgc attttgggtt tacaaaaagt aaaacttaaa caaaaattaa
     2461 aaagtatgta aatataatat gattagtata agaattttgt ttatggcata attttaggaa
     2521 cttgaaaaat aataaatgat atttacgctt tcaactttta gaagatttta ttaattatga
     2581 agaaaccatt tttaatgttg ttttttttaa taacttttta tgctgttctt gcacaagaaa
     2641 agtttagcga tgcaatttat tatcctgaaa ttaaaactgt acaattatac cagcaaaaca
     2701 accaaatgag cttacctatt atcaatttag gctcatcaga tttgttagaa ttgcattttg
     2761 atgatttaga tggatatgtg aaaaactact cttatacttt tcagctttgc aatgaaaatt
     2821 gggagccagt agatttaagc ccttttgatt atattaaagg gtttattcaa aaccggttaa
     2881 atcaatatag agtctcatct attgctcaaa ccaagtatgt tcattatcaa gcattgttgc
     2941 cagatagagg gagtgtgcca actaaaagtg ggaattattt attaaaagtg tttttaaatg
     3001 gagatattaa taaggttgca tttacaaaaa aattctttgt tttagataat aaagcttcta
     3061 ttgggttaca agttctacaa ccttatgata actctttatt tagaacttat cagcgtgtac
     3121 aagtaagtgt taatgctaca caactaaacc ctataaaccc acagcaacaa gtaaaagttg
     3181 tagtgttaca aaataaccga tgggataatg tggtaagaaa tattaaacca gcttttataa
     3241 gaggaaatgt tttagaatac aatggagagc aagattgctt atttgctgca ggaaaagaat
     3301 accgatgggt agatttaaga agttttagaa tagctacaga acgagtgcat agtattgata
     3361 aattaactat acccaatgaa attattttaa aacctgattt aacacgttct caattaaggt
     3421 atgcttactt taaagatttt aatggatggt attttatttc aagttcagaa aatatcaatc
     3481 ctttttggca ggcagattat gccaatgtac actttacttt tataccacca aacaataaag
     3541 ctatagaagg caaagatgtg tatataatga gcaattttac taatagtgag ctaaacgatt
     3601 ctacaaaatt attgtataat gaagaggaag ggtggtatga aaaaactttt ttaatgaagc
     3661 aaggttatta ctcttataca tacgttacaa aagataaaaa caacgaaaat gccattgcag
     3721 atgttaccct tactgatgga aatttatggg aaacagaaaa tgattatact gtatttgtat
     3781 attataaatc ggtaaatgga aggcatgatg agttagttgg tataactact gtaaatacaa
     3841 gattactaaa caggtagtat tactttttta aaactatttt atttgttacc aagtacagaa
     3901 tgggaaaaaa taatgcacta agcatagcaa ttaataacaa acctgaaatg tttttttctt
     3961 tcaacattct aattacgtaa ggtactttag aaggttttat ccattgttga taataacgta
     4021 aaaaggtact atcattagtg ctttgccata attgatatat ttggttggta agtattttat
     4081 ggtatttttt atctgctttg tgttgatggg catcgtaata tatttcacct ttacctgcat
     4141 tgtaagatgg tagcttaagt tttaatgcat ttataccttt ttcaaataag tataaagcag
     4201 aatcgctttt agtagttaac cataattttt ggataccttg aattgcataa atatgaccat
     4261 ctaaaatata aggtgtagtt acatttggtt ttgctatttc ctcataccac caagcagagt
     4321 cattaagctt ataagcaaaa ccattatttt gtactgttat ataaaaagca tggagtaaat
     4381 tttttgcaaa ctgcaaatag taattattgt tggtaaattg ataagcatat aaaaaaactt
     4441 caatagctct gccattggtc atgctactta tgaagggagg ggaaatgttt ggataccagc
     4501 cttgttgcca attaaattgg taaaaggtta aattattttg ttgcgtaaca gaatctttta
     4561 aaaactgaac acaatttaaa aattttttat agtaggtaga gtcagaattt tctaaaaatt
     4621 gttgagcata ttttattgca taattagcaa caatggtgga attaaaacga gtaatagcag
     4681 gaactccatt ttctgcaaca taatgtacat agggtacacc attagataat gtgtattctg
     4741 ttgccgtaga aggtacagag tctcctttta gaaagtataa agcttttctt gaattttcct
     4801 cccaatcatc tcctttaaaa ttactaacaa tactagcaat aaaaatagaa acaactataa
     4861 gtagaagtat atatgagaaa ttatttttca atatgtaaga ataatatcaa aaataatatt
     4921 tctactatac tatataaaaa tattgttata aagtaaagga ttaaacagct tctactttaa
     4981 ggcttttagc agccttatta cgttcgtttt cgtctaaaat aactttacgc atcctaatgt
     5041 ttttaggagt tacttcaata cattcatctt gctgaatata ttccatacat tcttctaaag
     5101 tcattaaaat tttaggcgta atacgaatac cttcatcaga gccactagct ctgtgattgg
     5161 taagcttttt accctcaata gcatttacat ttaagtctcc aggttttata tgctcagcca
     5221 ttacttgacc aatatacact tcttcacctg ggtcaacaaa aaatctacct ctgtcttgta
     5281 atttatctat agagtaagca gtagtagcac cttgaaattt agatattaaa acaccattat
     5341 ttctacctgg tattgcacct ttccatggtt tgtattcgct aaaacgatgt gccattacag
     5401 cttcgccagc agtgccagta agcatttgag aacgtaaacc tattaaccct cttgaaggga
     5461 tgtcaaattc taaatgttgc atttctccct tgctttccat taccaacatt tctcctttac
     5521 gttgtgttac taaatctatt actttaccac tgtattcact tggtacatct accactaata
     5581 cttcgtaagg ttcatgtttt ttaccattaa tttgttttac aattacttga gggttaccaa
     5641 ctgttaattc atacccttct ctacgcatag tttctactaa aacacctaaa tgtaatatgc
     5701 ctctgccata tactaaaaaa ctatctgcac tatctgtatc ttctactctt aaagctaaat
     5761 tcttttctgt ttctttcatt aacctatccc ttaagtgacg gcttgtaaca aattttccat
     5821 ctttaccaaa aaagggtgag ttgttaatac taaaagtcat gctcatagta ggctcatcaa
     5881 cgcttataat tggtaatgct tcagggtttt cagcatcagc aatggtatcg ccaatattaa
     5941 aatcttctaa gccaactaca gcacataaat caccagcttt tacttcattt actctgcttt
     6001 tgcccattcc ttcaaaaaca tataattctt ttacacggct ttttttaatt gttccatctg
     6061 cctgcactaa tgcaataggc atattttctt ttatagagcc tctggtaact ttaccaatag
     6121 caattcttcc taagaaagaa gaataatcga gagaagttat ttgaagttgt aatgcacctt
     6181 cagcaacttt aggagcagga acatatttta aaataccatc cattaaaggc aaaatgttat
     6241 ctgtaggtgt taagctatca ttaaaccagc cattttttcc actaccataa aatgtaggaa
     6301 aatttaattg ttcttcggtt gcatctaaat taaagaaaag ttcaaaaact gcatcatgca
     6361 cttcatctgg gcggcagttt ggtttatcta ctttattaat aactacaata ggatgtaggt
     6421 ttaattgtaa tgctttttgc aatacaaaac gtgtttgtgg cataggtcct tcaaaagcat
     6481 ctactaaaag tattacccca tcagccattt tcaaaactct ttcaacctca ccgccaaaat
     6541 cactgtggcc tggggtatct attacattta ttttaacgcc attatattta acagcagcat
     6601 ttttgctaaa aatggtaatt cctctttctc tttctaaatc attattatcc attattaact
     6661 cacctgagtc ttggttttct ctgaaaattt tagttgcatg taaaatttta tcaaccaaag
     6721 ttgttttacc atgatctacg tgtgcaataa ttgcaatgtt gcgtatttcc atatagtagt
     6781 ctaattatga ctttaatttt ttagaattag taaaaaataa aataaaccaa gcttttataa
     6841 aaggcgaagt gaaattttag ggctgcgaag ttagggcttt ctattgtttt ggtaaataaa
     6901 aagttttaaa atattgataa ttaatttatt atagctaacg aaggtaaatg ttttgaagaa
     6961 agttgttata agttattttc ctcctcggtt aactgttttt tatataaggc tgcatagtat
     7021 ccattaagag ccataagagt ttcatgagtg ccagcttcta taattttccc ttcattaaga
     7081 actataattt tatcaaattt aaaagatgaa aatattctat gggttataat tattgcagtt
     7141 ttattttgaa gaaaggtata aaaattttgg atgatagtat tttcagtttt agcgtcaaca
     7201 gcatttaagc aatcatcaaa aacaattatt tcagggcttt taagtaaagc cctagcaatg
     7261 gaaattcttt gcttttgacc accacttaat gtaacacctc tttcgccaat aatagtttca
     7321 tatccatttt caaactctaa aatatcgttg tgaacagaag ctaattttgc tacattttgt
     7381 attaattcca tttctgcctc attacttaaa ccaaagttta tattgttttt tatactatcg
     7441 ctaaataaaa aaacatcttg tggaacataa ctaatttgtt ctcttagttt atataagttt
     7501 aaagagttta agttattatt tccaattgta atagagccag ttttagggtt gtgaaatctt
     7561 aaaagcaatt gtgctaaagt tgattttcca cttcctgttt tacctaacac taatacttta
     7621 gagcctttgt taatggttaa attaaaattt tttatagctt ctattcctgt attttgataa
     7681 ataaaagaaa cattattaaa aacaatatcg cctgaaatat ctatttcagt tttgttggaa
     7741 ttatttttaa tttcaggttc tatttctaaa aaattattta ttcttttttg agaagcagct
     7801 gctcgttgaa taatgcttgc tgtccaacca attgcactta caggaaaagt aagcatgttt
     7861 atataaatta caaattctgt aattgttcct gcagttacac cattgttttt gtgaattaca
     7921 taccaactgc caatggcaat tgtaagcaat gtacttatgc caataattaa tgccattgat
     7981 ggaaaataga aggcttctaa ctttgctaaa ctaatagcac tttgtttgta ctcattactt
     8041 ttcttagaaa agaaattggt aattgcattt tcctgcacaa aagattttaa tactctaatt
     8101 ccagaataag cttcttgtgc cgaggaagtt aaattagata attgctcttg tattttttca
     8161 ctctttttat taataaccga atttactaca taaataataa aagcaagaaa gggtaaagga
     8221 gatagaacat ataatgttaa ctcaatatta gctttaagca taaaaaaaat actgaagcca
     8281 atagtaaaag caaggtttat aatatacatt actgcaggcc cggaatacat tctaactctg
     8341 cttacatctt cagaaattct gttcattaaa tcgcctgtag catgagcttt aaaaaagctt
     8401 gtatctaacg ataaatactt tttaaatact tcattttttt gggcaaattc tatatgcctg
     8461 ctcataacaa taatagtttg cctcattaaa aacataaata aaccacctaa aattgcaaat
     8521 actaataacg aaacggaaca aagaacaact atttcactta atgaaaattg ttttgtagta
     8581 agccaattaa taagcatcaa aaccaaataa ttatagttat actgcttggg ggcaactgtg
     8641 tgtatttgtt gattggttgc aagtttttct tgcacagcat ctacaatata cccagttatt
     8701 tgaggggtta aaattctaaa ataattagat actataatac aaaaaatacc tgcaataaat
     8761 agttttttgt atttccagaa atatttattt aatgctgcta aatgtttcaa aaattaacga
     8821 atgatttata acgaagttag tagaagaaaa ataatttttt ggcagataaa ctttcaacct
     8881 attattttgc aaccggagag atggcagagc ggtcgaatgc ggcggtcttg aaaaccgttg
     8941 actgtaacag gtccgggggt tcgaatccct
//
LOCUS       DAOQHP010000045         8534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4216_length_8534_cov_3.925817, whole genome shotgun sequence.
ACCESSION   DAOQHP010000045 DAOQHP010000000
VERSION     DAOQHP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4216_length_8534_cov_3.925817"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3181
                     /locus_tag="PLY81_02620"
     CDS             <1..3181
                     /locus_tag="PLY81_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNF29154.1"
                     /translation="YDSLQQAPLVNIEDKTWMKNVLHIGGATDYNIGTSITNYLNKYT
                     QIIEDTLFGAKVKKYNKLDNPAGYGAALVDFKNTYESGASIITYFGHSSANLLDFNLD
                     NPNAYNNPNKYPVFIVNGCDAGNFLTYEPQRLNIKTSISEKFVLAPERGAIGYLATTG
                     YGAINYLDTFTTGFYRALSTIDYNKPFGESIKDGIINVLNTTGLNDFFARYHAEQFIF
                     HGDPALKLNTFPLPDLVLKTEYTTTLPTKITVAKDSFYLKMDIYNIGKKINDSINLKI
                     TRQYPKGNSEVVLNKKLPAIALMTTETAAFPIIANRDKGTTILTATIDATNSINEMDE
                     NNNTAVISVSITDDEIVPIYPYNYAIVNNSNFKLAASTANPLDTLKNYIVEVDTTSLF
                     NSPIKYTQNKSSVGGVVEFDYGLTLQNNTTYFWRVATQGSSYWNTVSFTYKSSPDIGF
                     EQRHFLQHTDSKLTDIILDSSSRKFKFGQTLNNLFVVHSIYPYSGTEDQHFSIKVNGT
                     GIIASACLGNSVIINVFDTLSFNPWINTTNPFGATPTCLETRKYNFEYSYLDVSGRNN
                     AKSFLESIPNGMLVAVRMVYDGASVWANDWANDTLLNGSGNSLYHFLKNQGFAIDSFN
                     HPRTFAFIFKKNDSLHFTPKYLFSEGLYDRVILNADYNSDDTLGYVVSPKFGPAKAWK
                     FAKWNGNGNGSSFAKMNIIGIDTNGIENVLFNNMDTLQTSFDISSINAQQYPFVKLSI
                     LNQDSVKAFPYQLSNWSVEYVGVPEGAVAPNLHINIPDTIGLTSSYASDTLKGSFAFK
                     NVSKENFDSLTVKVVLTNLRLGTVHTFNLAKTKPLLAGDTLHINFAIDASLMSRDEYN
                     LYVAVNENAAQPEQYLFNNFLYKYIYFKTSVIVPVKLLNLTAKINGTGTDITWQVAEE
                     VNVLKYEVEHSNNAIDFKTIGSVDANGKNNYLLHHAQPTYGKNYYRLKILNTDGSYNY
                     STIRLVNFGKGVIVNVYPNPVIGKLNIAVSNNNNKQVQVNLYNNYGQLLFTEEFKNNS
                     TIDMSKLSSGVYILQVNDGIQWQTFRIVK"
     gene            complement(3255..4304)
                     /gene="queA"
                     /locus_tag="PLY81_02625"
     CDS             complement(3255..4304)
                     /gene="queA"
                     /locus_tag="PLY81_02625"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNF29155.1"
                     /translation="MKLSQFRYDLPLNLIAQHPTKKREDSRLLVVNRKTGNMENKHFR
                     DVLDYFDDKDAFVVNNTKVFSARMYGRKEKTGAKIEVFLLRELSSQNRLWDVIVDPAR
                     KIRVGNKLYFGESEELVAEVIDNTTSRGRTIKFLWEDTEESFRKMLETLGETPLPKYI
                     KRKPDEEDKERYQTVYAKHEGAVAAPTAGLHFSKELIKRCEIKGIRFAEITLHTGLGT
                     FRPIEVEDLSKYKMDAEYYNIPEGAVQIVNKAKDNGHRVCAIGATTMRALETNFTAQK
                     RLKSSEGWSNHFIHPPYDFSIADSMVTNFHLPKTSLFIMTCAFAGYDLTMEAYHKAIK
                     DKYRFYSYGDVLLII"
     gene            complement(4373..4585)
                     /locus_tag="PLY81_02630"
     CDS             complement(4373..4585)
                     /locus_tag="PLY81_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNF29156.1"
                     /translation="MDQTILEDLVSKTIPFGKYKGKIYCELPEVYIEWFARKGFPKGR
                     VGTLLQTMYEINLNGLQHLLNPLKKK"
     gene            complement(4610..7435)
                     /locus_tag="PLY81_02635"
     CDS             complement(4610..7435)
                     /locus_tag="PLY81_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNF29157.1"
                     /translation="MKNKLGKIFKYSGISIGSILVLMFLLPYLFPGFVSDKIKKWANK
                     SIESELNFSKARLSFFNHFPSLTLTLYDVNLKGSAPFKNDTLITANEIALGVDILSVF
                     KNTLKIDEVYVTNGKIKILVSKNGIPNYNIYKSSSTTKEHSEVHEESDAALKLDRIQI
                     ENCALEYNDQSLPMLLKAGKVNYLGQGDLSKEIFDLTSKIEMQDFDFIYNTQPYLSNK
                     NLKAQLITRINTHSLELDFQKNNLNINHLPIEVTGKLNFLSNGYSLQFNANTPSTKLK
                     NLFTALPPAFVQWVDKTNVDGKLALNVALNGNYIKSKNQAPDLKFTIQVNNGSFSIAN
                     SNEPIKNIYLNASIDIPKLNTQNLFLKVDTLNATLGKNYLKGAFEAKNFAEPFIKGHL
                     NVNMDLEKWNNILNIDSIAGITLKGNFKTEANFNGVYNTQKNTFPITNATIQWQNGYL
                     KTKYYASAINNINIDATIQNKSGTTKDVLINVKPISFLFEEQPITVSANLLNLNNLNY
                     SISSKGNLQIGKLYKLFAIDGYNINGNIKTDFTLQGSQQDALAGNYHKLNNKGTIELK
                     DINLTTPYLPNKFTITNGNFYFNNDKLIANNVTINYLNNTAVFKGKFDNIINYLLQPN
                     AALKADLFLNSNKLNVQDFMAFAKDSTTLINTNKTEKNSTPTSNSTGVILLPTNLNIN
                     FNAAIKTVEYNHLQLSNVLTNASIQNGNLKIHNADFNVIGSPIKMTAKYSPINFKKAH
                     FTYNISATELDIQKAYKEIQFFRDMLTSAKKMKGLVSINYSINGLLNDSLYPILPTLK
                     GNGTISIKNAKFYGFKLFNAISKKTNKDAIDNPDLSKHNIILKSKIANNIITIEKIKL
                     RVAGFRPKFEGQVSLDGRLNLKARLGLPPFGIFGIPLSITGTQENPIVKFKRGKKSTD
                     LEETEADEEDKKDAAEAEQKEQQQKNK"
     gene            complement(7512..8225)
                     /gene="pyrH"
                     /locus_tag="PLY81_02640"
     CDS             complement(7512..8225)
                     /gene="pyrH"
                     /locus_tag="PLY81_02640"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNF29158.1"
                     /translation="MLPKYKRVLLKLSGEALLGQNNGDPFDPKIIEQYAYDIKEVTNL
                     GVQVAIVIGGGNIYRGMNEADSGIERAHGDYMGMLATVINAMAMQAMLEKIGVYTRLQ
                     SAINMEQVAEPYIRRKALRHLEKGRVVIFGAGTGNPYFTTDTAGSLRAIEMKADVILK
                     GTRVDGIFTADPEKDATATKYDKLSFKECITNNLKVMDMTAFTLCMENKLPIIVFNMN
                     KPGNLLNVVEGLNVGTLVN"
BASE COUNT         2877 a         1255 c         1219 g         3183 t
ORIGIN      
        1 atacgattca ttacaacaag ccccacttgt gaatatagaa gataaaactt ggatgaaaaa
       61 tgtattgcat attggtggag caacagacta caatattggc acttctataa ccaattattt
      121 aaataaatat acccaaataa tagaggatac tttatttggt gcaaaggtta aaaaatataa
      181 taaattagat aatcctgctg gttatggtgc tgcattagtt gactttaaaa atacttacga
      241 gtcaggtgca tctataataa cttattttgg acactcatcg gcaaatttat tagattttaa
      301 tttagacaat cctaacgctt ataataaccc taataaatat cctgtattta tagttaatgg
      361 atgcgatgca ggaaattttt tgacttacga accacaaaga ctaaacataa aaacaagtat
      421 ttcagaaaag tttgttctag caccagaaag aggtgcaatt ggttatttag caacaacagg
      481 ctatggtgca attaattatt tagatacatt tactacaggc ttttatagag ccttaagtac
      541 tatagattat aataagccat ttggagaaag cataaaagat ggaattatta atgtattaaa
      601 cacaacaggc ttaaacgatt tttttgcaag gtatcatgca gaacaattta tttttcatgg
      661 agaccctgct ttaaaactta atacttttcc tttaccagat cttgttttaa aaacagaata
      721 cacaactacc ttaccaacaa aaattacagt tgctaaagat tcgttttatt taaaaatgga
      781 tatttataac attggaaaaa aaataaatga ctctataaat ttaaaaataa cacgtcaata
      841 tcctaaaggc aatagcgaag ttgtacttaa caaaaaacta cctgccatag cattaatgac
      901 aactgaaaca gcagcttttc ctatcattgc caatagagat aaaggaacca ctattttaac
      961 tgctacaata gatgctacca atagcattaa tgaaatggat gaaaataaca atacagctgt
     1021 aatttcagta tccattacag atgatgaaat tgttccaata tacccctata attatgctat
     1081 agtaaacaat tctaatttta aattagcagc ctctactgca aacccacttg ataccttaaa
     1141 aaattatatt gtagaagtag atactacttc cttattcaat tcacccatta aatacacaca
     1201 aaataaatca tcggttggtg gtgttgttga atttgattat ggattaacct tacaaaacaa
     1261 tacaacttat ttttggagag tagcaacaca aggaagtagt tattggaaca ctgtttcgtt
     1321 tacttataaa agcagcccag atattggctt tgaacaaaga cactttcttc aacatacaga
     1381 tagtaaacta acagatatta ttttagatag tagctccaga aaattcaaat ttgggcaaac
     1441 tttaaataac ctctttgttg ttcattctat ttatccttat agtggaacgg aagatcaaca
     1501 ttttagtata aaagtaaatg gtacaggtat tattgcaagt gcttgtttag gtaattctgt
     1561 aattataaat gtttttgata ctttaagttt taacccatgg ataaatacta ccaatccgtt
     1621 tggtgctaca ccaacttgcc tagaaacaag aaaatataat tttgaatatt cttatttaga
     1681 tgtatcaggt agaaataatg ccaaaagctt tttagaatct ataccaaatg gcatgttagt
     1741 agctgttcgt atggtgtatg atggagcctc tgtttgggca aatgattggg ccaatgatac
     1801 attattgaat ggctcaggta attccttgta tcatttctta aaaaatcaag gctttgcaat
     1861 agattctttc aatcatccaa gaacctttgc ttttattttt aagaaaaacg actcgttaca
     1921 ttttactcca aaatatttat ttagtgaagg attatatgat agggttattt taaatgcaga
     1981 ttataatagt gatgacactt taggctatgt tgtttctcca aaatttggac ctgcaaaagc
     2041 atggaaattt gcaaaatgga atggcaatgg caatggtagc agttttgcta aaatgaatat
     2101 tattggaatt gatactaacg gtattgaaaa tgtattattt aataatatgg atactttgca
     2161 aacaagtttt gatataagta gtattaatgc acaacaatat cctttcgtga aattatctat
     2221 attaaatcaa gactctgtaa aagcatttcc ttatcagctt agtaattgga gtgttgaata
     2281 tgttggtgtg ccagaaggtg cagttgctcc taatttacat atcaatattc ctgatacaat
     2341 tggtttaaca tcaagctatg caagcgatac tttaaaaggc agctttgctt ttaaaaatgt
     2401 aagtaaagaa aattttgata gtttaacagt aaaagtagtt ttaactaatt taagattagg
     2461 aactgttcat acatttaacc ttgctaaaac aaaaccatta cttgcagggg atacattgca
     2521 tattaatttt gctatagatg cctcgttaat gtcaagagat gaatataact tgtatgtagc
     2581 agttaatgaa aatgcagctc aaccagaaca atatttattt aataactttt tgtataagta
     2641 tatttatttt aaaacaagtg taattgttcc tgtaaaatta ttaaatctca ctgcaaaaat
     2701 taatggtaca ggaacagata taacctggca agttgcagag gaagttaatg ttttaaaata
     2761 tgaagttgaa catagtaata atgcaattga ttttaaaact ataggtagtg tagatgccaa
     2821 tggtaaaaac aattatttgt tacatcatgc acaacctacc tatggtaaaa attattacag
     2881 gctaaaaatt ttaaatacag atggtagcta taactattct accatacgac ttgtaaattt
     2941 tggaaaagga gttatagtaa atgtgtatcc taaccctgta ataggcaaat taaatattgc
     3001 agttagtaat aataataaca agcaagtaca agtaaactta tataacaatt acgggcagtt
     3061 attatttaca gaagaattta aaaataattc aactattgat atgagtaaat tatcatcagg
     3121 tgtatatatt ttacaagtaa atgatggtat tcaatggcaa acttttagaa tagtaaaata
     3181 attagattca tataataaaa aatggtgtac aacaatattt gtacaccatt tttttataaa
     3241 aataattagt agaattagat aattaataat acatctccat agctataaaa acgatattta
     3301 tcttttatag ctttatgata agcttccata gttaaatcat aacctgcaaa tgcacaagtc
     3361 ataataaata aacttgtttt aggtaaatga aaattagtta ccatactatc tgcaatacta
     3421 aaatcataag gagggtgaat aaaatggttt gaccaacctt cagaagattt taatcttttt
     3481 tgagcagtaa aattagtttc taatgccctc atagtagttg caccaattgc acaaacacga
     3541 tggccgttat ctttggcttt atttacaatt tgaactgcac cttctggtat attgtaatac
     3601 tcagcatcca ttttatattt actaaggtct tccacttcaa ttggtctaaa agtacctaag
     3661 cctgtatgta aagtaatttc tgcaaaccta atacctttta tttcacaacg tttaattaac
     3721 tctttactaa agtgcaagcc agcagttggt gctgcaacag caccttcgtg ttttgcataa
     3781 acagtttgat acctttcttt gtcctcttca tctggcttac gtttaatgta ttttggtaat
     3841 ggagtttcgc ccaaggtttc taacattttt ctaaaacttt cttctgtatc ttcccataaa
     3901 aattttattg ttcttccacg gctggttgta ttgtcaatta cttctgcaac aagttcttca
     3961 ctttcgccaa aatatagttt attgcctaca cgtattttac gagcagggtc aacaattaca
     4021 tcccataaac ggttttggct acttaattct ctaagtaaaa aaacttcaat ttttgcaccg
     4081 gttttttctt ttctgccata catacgagca gagaaaactt tagtgttgtt tactacaaac
     4141 gcatctttat cgtcaaaata atctaaaaca tcacggaagt gtttattttc catgttacct
     4201 gttttacgat ttacgactaa taaacggctg tcttcacgtt ttttggttgg gtgttgtgca
     4261 ataaggttta aaggtagatc gtacctgaat tgagataatt tcatgttacg gcatgattta
     4321 gttaaaaaat gaaaggctgc aaaagtaata gatttatagg tataaaactt ttttacttct
     4381 ttttcaacgg atttaataga tgttgtaagc cgtttaggtt tatttcgtac atggtttgta
     4441 gcaatgttcc aactcttcct ttaggaaagc cttttcttgc aaaccattca atataaactt
     4501 ctggcaattc acaataaatt tttcctttgt atttgccgaa tggtatggtt ttacttacta
     4561 gatcttctaa aatagtttga tccatagata ttaagagggc taaattgtat tatttatttt
     4621 tttgttgttg ttctttttgt tctgcttctg cagcatcttt tttatcttct tcatccgctt
     4681 cggtttcttc taaatcggtt gatttttttc ctcgtttaaa tttcacaatt ggattttctt
     4741 gtgtgccagt aatagaaagt ggaatgccaa atatgccaaa tggtggcaag cctaatctgg
     4801 cttttaaatt taacctacca tctaaactaa cctgcccttc aaactttggc ctaaagccgg
     4861 caactcgtag ttttattttt tcaatggtaa taatattatt agcaattttt gattttagta
     4921 ttatgttatg tttagataaa tccgggttat ctattgcatc tttatttgtt tttttactaa
     4981 ttgcattaaa caacttaaaa ccataaaatt ttgcattttt aatacttatt gttccattac
     5041 cttttaaggt tggtaaaatg gggtataagc tatcattcaa taacccattt atagaataat
     5101 taatgctaac cagacctttc attttttttg cagaagttaa catgtctcta aagaattgaa
     5161 tttctttata tgctttttga atgtctaatt ctgtagcaga aatgttgtaa gtaaaatgtg
     5221 cttttttgaa gttaatgggg gaatattttg cagtcatttt tattggtgag ccaataacat
     5281 taaaatctgc attatgtatt tttaaatttc cattttgtat gcttgcattg gttaaaacat
     5341 tactaagttg taagtggtta tattcaacag ttttaatggc agcattaaag ttaatgttta
     5401 agtttgtagg aagtaaaata accccagtag agtttgaggt tggtgtgcta tttttttctg
     5461 ttttattagt gttgattaag gttgtagaat cttttgcaaa agccataaaa tcctgcacat
     5521 ttaacttgtt actatttaaa aacaaatctg cttttaatgc tgcattgggt tgtaataagt
     5581 aattaataat attatcgaac ttgcctttaa aaacagccgt attgtttaaa taatttatag
     5641 ttacattatt ggctattaac ttatcattat taaaataaaa attaccattt gttatagtaa
     5701 acttattagg taaataaggt gttgttaaat taatatcttt aagttctata gtgcccttat
     5761 tgtttagttt atgatagttt cctgcaagtg catcttgttg tgagccttgt aaggtaaagt
     5821 ctgtttttat atttccgttt atattgtaac catctattgc aaacaattta tacaacttac
     5881 ctatttgcaa atttcctttt gatgaaatgg aatagttaag attatttaaa ttaagtaagt
     5941 tggcacttac tgttattggc tgctcttcaa ataaaaaaga aatgggttta acatttatta
     6001 gtacatcttt ggtagttcct gatttatttt gaatggttgc atctatatta atattattaa
     6061 ttgcactggc atagtatttt gtttttaaat atccattttg ccattgtata gtagcatttg
     6121 ttattggaaa tgtatttttt tgggtattat aaactccgtt aaaatttgct tctgttttaa
     6181 aatttccttt taatgttatg cctgcaatgc tatctatgtt taaaatatta ttccattttt
     6241 ctaaatccat attaacattt aaatgcccct taataaatgg ttcagcaaaa ttttttgctt
     6301 caaaagcacc tttcaaatag ttttttccaa gtgtagcatt taaagtatct acttttaaaa
     6361 ataaattttg ggtgtttagt tttggtatat caatggaggc atttaaataa atatttttga
     6421 taggttcgtt gctgtttgca atactaaatg aaccattgtt tacttggata gtaaatttta
     6481 aatcgggtgc ctggttttta cttttaatat agtttccgtt taatgctaca tttaaggcaa
     6541 gttttccatc tacattagtt ttatcaaccc attgaacaaa agcaggagga agtgcggtaa
     6601 acaaattttt taattttgta gaaggtgtat tggcattaaa ttgcaagcta tatccattac
     6661 ttaaaaaatt taattttcct gtaacttcaa ttggtaaatg atttatgttt aaattatttt
     6721 tttgaaaatc taactctaaa gaatgtgtat taatacgtgt tattagctgt gctttaagat
     6781 ttttgttact taagtaaggt tgtgtattat aaataaaatc aaaatcttgc atttctattt
     6841 tagaggtaag atcaaaaatt tctttactta aatctccttg tcctaaataa tttactttac
     6901 cagcttttaa cagcattggt aaagattgat cgttatattc taaagcacag ttttctattt
     6961 gtattctatc tagttttagt gcagcatcgc tttcttcatg tacctcagaa tgttctttgg
     7021 ttgttgaact gcttttataa atattataat taggaattcc gtttttacta actaaaattt
     7081 taattttacc attggttaca taaacctcat ctatttttaa agtgttttta aatacactta
     7141 aaatatctac acccaatgca atttcattag ctgtaattaa tgtatcattt ttaaatggtg
     7201 cagaaccttt taaattaaca tcatataaag taagagttaa agaaggaaag tgattgaaga
     7261 aggataatct tgctttagag aaatttaatt cagattcaat agatttatta gcccattttt
     7321 ttattttatc agatacaaac cctggaaata agtaaggtaa taaaaacatt aataccaaaa
     7381 tgctaccaat acttattcct gaatatttga atattttacc taatttattt ttcattataa
     7441 gcctgtataa aatagaaaac tgttagcaaa agtactaaca gtttttaaag aatattttta
     7501 ctattattta attaatttac caatgtacct acatttaaac cttctactac atttagcaaa
     7561 ttaccaggtt tgttcatatt aaaaacaata attggcaatt tattttccat acacaaagta
     7621 aaagcagtca tatccattac ttttaaattg tttgtaatac attctttaaa actaagttta
     7681 tcatatttag tagctgtagc atctttttca gggtcggcag taaaaatacc atctactctt
     7741 gtgcctttta aaatcacatc tgctttcatt tcaatggctc ttaagctacc agcagtatct
     7801 gttgtaaaat aagggttacc tgttcctgct ccaaaaatta caacacgccc tttttctaaa
     7861 tgtcttaaag cttttcttct aatatatggc tctgccactt gttccatatt aatagcactt
     7921 tgcaaacggg tgtacacacc aattttttct aacatagcct gcatagccat agcattaatt
     7981 acagtagcca acatacccat ataatctcca tgtgcacgtt caataccact atcagcttca
     8041 ttcatacctc tatatatgtt tccacctcca ataacaattg caacctgaac tcctaaattg
     8101 gttacttctt ttatatcata agcatattgt tcaattattt taggatcaaa tggatctcca
     8161 ttattttgtc ctaataaagc ttctccagat aattttaata atactctttt gtatttaggc
     8221 aacatgttat aatatttatg tgtgcaaata accactattt ttattatgca gccaatagta
     8281 ttgtaataaa tttaattata taggctattt aagttaacta tttgcccttt gttttgttag
     8341 tttttagctt aaaatagcta aaagatagag cagaaatatt ttttttactg atttatataa
     8401 taaattttac tagctatcgt tttaatataa cagccccaaa gcaattagtt taaaggctga
     8461 aatatttaat atccaatatc aaacattcta tgaaatccaa tttctacacg ctaataatgc
     8521 gtttaacctt ttgc
//
LOCUS       DAOQHP010000046         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4260_length_8479_cov_3.804012, whole genome shotgun sequence.
ACCESSION   DAOQHP010000046 DAOQHP010000000
VERSION     DAOQHP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4260_length_8479_cov_3.804012"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..938
                     /locus_tag="PLY81_02645"
     CDS             216..938
                     /locus_tag="PLY81_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNF29159.1"
                     /translation="MNKKYYIFFLLCFIISCKSNKQIFSKNEYGLIVLTTPKQYFSTV
                     SLDSSKLLKRLNGFIPNIVFDWRYATTNNFTKQILYTNPDAYLRIEAANNLKNVAIEL
                     EKIGLGIKVFDAYRPYSVTKKMWEVVPDDRYASDPAKGSGHNRGIAIDLTLYNLETGT
                     ELEMPTSYDNFTENAHANYQQLSQIVLKNRTILINTMQMFGFKVLDTEWWHFYLPNSS
                     KYELMDYDFKTMSKMCNVNTHK"
     gene            complement(995..2527)
                     /locus_tag="PLY81_02650"
     CDS             complement(995..2527)
                     /locus_tag="PLY81_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNF29160.1"
                     /translation="MNSNLKNLFTVVIVSAATAVLSVVGYDKYMQNKYAGTQEEGKLP
                     VNYARFFDNTNAPIGADFSLAATSATPAVVHIKTKTKSKQISNNAPRSRNPFSDMFGG
                     DMFDEFFNGPRMQVIPEQQASGSGVIVSKDGYIVTNNHVVDGADEIKVTLTDKKTYTA
                     SIIGTDPSTDLAVIKIEGNNFPYLIYGNSDEVKLGQWVLAIGYPLNLDVTVSAGIVSA
                     KARSININQRQSKTPIESFIQTDAAVNPGNSGGALINTNGELVGINSAIASPTGYYTG
                     YAYAIPVNIVKKVIADMVKYGSVQRAFIGISYPPEGIGEKQQEQFDKEYGTAWKTNEG
                     VFVIGVPEDGAAYAAGIKEKDIITKVNGVATPSGPQLQEQVSLYKPGDKVKITLKRNN
                     KEMVVSVTLKNKAGNTEIVKKSTILDKIGGTLETIDSKIAAANDIQGGVVVKKIGTGA
                     LSKTRVQEGFVITSVNGIEIKNLEDLKSALDKADGRIRLEGIYPGYEGSYTYPLNINN
                     ED"
     gene            complement(2873..3220)
                     /locus_tag="PLY81_02655"
     CDS             complement(2873..3220)
                     /locus_tag="PLY81_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF29161.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAITDGTTVKGDFT
                     LLNGTTIDVEQRNEPISVDTYFIVPDKCTECQGFHEEPQCAAVCPVDCCVPDEMYRET
                     VEELLAKKDKMHI"
     gene            3311..4318
                     /locus_tag="PLY81_02660"
     CDS             3311..4318
                     /locus_tag="PLY81_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNF29162.1"
                     /translation="MILQERINLLKKLNNYFLENNIELQNVKQKAFQENAWFIPEFVE
                     ESIKNISNEFLEENKLNNLIQQYKIPAENIQPKRIGIVMAGNIALVGFHDFLCAFITG
                     HSVVIKPSSKDSVLIKHIVQKLAEWNSEINSIISFAEILKGCDAFIATGSNNSSRYFE
                     YYFEKYPSIIRKNRTSVAILTGKESKTELDLLAKDIQLYFGLGCRNVTKLFVPEKYDF
                     IPLLDALKKYDYYLEYHKYKHNYDYHLALLIMGNKLYMNNGSIVFTENESNYSPISQV
                     HYSYYKKNEIDYLLENIQPNQEIQCIIGYQGVPFGCSQQPCITDFADGVDTIQFLINL
                     N"
     gene            complement(4315..5421)
                     /locus_tag="PLY81_02665"
     CDS             complement(4315..5421)
                     /locus_tag="PLY81_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF29163.1"
                     /translation="MKRVYTINKIKSTFVLYPISLKMRNRIHFTTIIVLFMLFGKQQN
                     VQAQDVHFSQFFEAPFLRNPSFSGLFQGDIRFQGIYRSQWASVTTPFTTGSFNFEYKK
                     PIGGADDFLTLGMQMLYDKAGSASLTTTSFLPALNYHKALSGEKNKYLSLGIMGGFVQ
                     RKFDRSKITTNSHYDGNGYNPSLPDGETFLNSNFNYLDGSIGLSFNSTIGGKAEDNYF
                     IGVAYHHLNRPKNSFYKNPDIELQAKMVVSTGIKFSLNETSYFTIHADYSKQGSFTEL
                     IGGAMYSYKIGDDIDNPKYTFHVGAFMRAKDAFIPMIKLDYHPFAFSFSYDANVSQLR
                     TASQGRGAMEFSISYTGFFDRDNSSKFVPLCPKF"
     gene            complement(5434..>8479)
                     /locus_tag="PLY81_02670"
     CDS             complement(5434..>8479)
                     /locus_tag="PLY81_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF29164.1"
                     /translation="KDTLQLNNYIYINPPIALFYTVFSCDTPLTRTFIDQSKGAGLTY
                     LWDFGDGNTSTAQSPTHTYATYGIYNVKLTVTNGVCTHSATAPANLSFNNPILVTTDT
                     AFCKYALATYKINNIVPSLITSYVWDFGDGTIMSGSNIDSVTHAYTTNGNVAPTVIIN
                     DVAGCADTLTISPPIKIFGPNANFVNQPGTCLYDTINFTNTSTTDGIHNLTQVIWNYG
                     DGITDTLTSPPYQHQYNATGFFTVKLKVLDDFGCYDTLIKPNAVQITKPIAGFFSNDT
                     IRCANSNVTFQDTSKGVNLTYLWNFGDTQTATQQNPQHPYLLQGLYSVKLKVTDIFGC
                     VDTLLKPLYIRVANSVANFKFLQGDTLGLCYPFLIETANLSSDAVSVNWSFGDNGFSN
                     LDTPSHIYTYAGKYTLKLKAYGYGGCVDSTQKDIIVRGPTGTFSYSPLQFCSPTVVNF
                     VAHTKNNATFVWDFADGVITLTPDSTISHTYNTPGIYRPGIIMIDSAGCQVPIIGTDT
                     VRVADVSTLIKVPQTNFCDSVQLNFLDSTIVYNDTVSTYLWNFGDGVTSTIKNPTHFY
                     NQTGSYKITLKVTTNLGCTNTDTLNVPINVVQTPSIAITGDSVSCINSLLQFNGAIIK
                     SDTNAISWQWNLANGNTSVLQNPTTQQYITAGTYNIKVIAFNIAGCADTVTKNILIHP
                     LPIINAGVDTFVCKGKTIKLNASGGVGYVWNMDSTLSCTNCASPNVFTQNSSIYKVTG
                     TSSFGCKNEDSVAVTVIEPFTFSISANDSICTGSSSQLLASGNADKFQWQPTTGLNNP
                     NIANPIATPTTTTVYTVIATDNKKCFADTGLVEIRVFPMPQFNIIESLVQSNVGNVVP
                     LLTSNSADITKWEWIPNQWLTCADCPTPNAIITDKIKYVAEASNPGGCFARDEVTIEP
                     LCNGYNVFIPNTFSPNNDGVNDVFYPRGKGLFTVRNMTIFNRWGEIVFTKRDFNANNA
                     ANGWDGTFNGKQLSADVYVYTIEVICANSQTMFLKGNITLLR"
BASE COUNT         2863 a         1348 c         1303 g         2965 t
ORIGIN      
        1 gttaagaaat ctttcataaa aaagttaaca cttagtgttg gccttttttt ataatcagct
       61 ttttgatttt gagcaaaaga agttgttgtt attaatccaa caacaaaaag taaaataatt
      121 ttcttcataa ttacatttta attaattgtt gcaaaattat gggtatatga taaaataaac
      181 gaattttaac tttgaatatc ttaataagtt tatacatgaa caaaaaatat tatatatttt
      241 tcctactttg ctttatcatt tcttgcaaat ctaataagca aatattttct aaaaatgagt
      301 atggtttaat agttctcacc actccaaaac aatatttttc taccgtatca ttagatagca
      361 gtaaactatt aaaaaggctt aatggtttta taccaaatat tgtttttgat tggagatatg
      421 caaccacaaa taattttaca aaacaaatat tatacacaaa cccagatgct tatttaagaa
      481 ttgaagcagc caataattta aaaaatgttg ctatagagtt agaaaaaata ggattaggaa
      541 taaaagtttt tgatgcttat cgtccttatt ctgttacaaa aaaaatgtgg gaggttgtgc
      601 cagacgatag atatgcatct gaccctgcaa aaggcagtgg gcataatcgg ggtattgcta
      661 tagacttaac attgtataat ttagaaacag gaactgaact tgaaatgcca acctcttacg
      721 ataattttac tgaaaatgct catgctaatt atcagcaact aagccaaata gttttaaaaa
      781 atagaacgat tttaataaat actatgcaaa tgtttggttt taaagtatta gatacggaat
      841 ggtggcattt ttatttacca aactcttcta aatacgaatt aatggattat gattttaaaa
      901 ctatgagcaa aatgtgtaat gtaaatactc ataaataaaa atgctaccta aaatttaggt
      961 agcattttat gtttaaaatg gtgagttaaa tttattagtc ttcgttgtta atgtttaaag
     1021 gatatgtgta agaaccttcg tagcctggat agataccttc taatctaatt cttccatcag
     1081 ctttatccaa tgcagatttt agatcctcta aattttttat ttcaatacca tttacagaag
     1141 taattacaaa gccctcttga acacgtgttt tggataatgc accagtacca atttttttaa
     1201 ccactacacc accttgtata tcatttgcag cagcaatttt tgaatcaatt gtttctaatg
     1261 ttcctccaat tttatctagt attgttgatt tttttactat ctcagtattt cctgctttat
     1321 ttttaagtgt tactgataca accatctctt tgttgtttct tttcaaagta atttttactt
     1381 tatctcctgg tttatataaa gatacttgct cttgtaattg tgggccacta ggagttgcta
     1441 caccatttac ttttgtaata atatcttttt cttttatacc agcagcataa gctgctccat
     1501 cttctggtac accaattaca aaaacacctt cattggtttt ccaggctgtg ccatattctt
     1561 tgtcaaactg ttcttgttgt ttttcaccaa taccttctgg tgggtaagaa atacctataa
     1621 aagctctttg aacagagcca tattttacca tgtcggcaat tacctttttt acaatattta
     1681 ctggtattgc ataagcatag cctgtataat agccagttgg agatgcaata gcactattga
     1741 tacctactaa ctctccatta gtatttatta aagctccacc actgttacct gggtttacag
     1801 cagcatctgt ttgtataaaa ctttcaatag gtgttttact ttggcgttga ttaatattaa
     1861 tgcttctagc ttttgcacta acaatgcctg cacttactgt tacatctaag tttaatggat
     1921 atccaatagc taaaacccat tgtccaagtt ttacttcatc actatttcca taaattaaat
     1981 aaggaaaatt attgccttca attttaatta ctgcaaggtc ggtacttggg tctgttccaa
     2041 taatactggc agtataagtt tttttatcag ttaaggtaac ttttatttca tcggcaccat
     2101 ctacaacatg gttattagta acaatatatc catctttact tacaattaca ccgctaccac
     2161 ttgcttgctg ttctggtatt acttgcatac gtggaccatt aaagaactca tcaaacatat
     2221 cgccaccaaa catatcacta aatgggtttc tgctacgagg ggcattattg cttatttgct
     2281 ttgattttgt tttggttttt atatgtacca cagcaggtgt agcacttgtt gctgctaaag
     2341 aaaaatctgc tccaattggg gcatttgtat tatcaaaaaa tcttgcatag tttacaggta
     2401 gtttaccttc ctcttgagtg cctgcatatt tgttttgcat atacttatca tatcctacca
     2461 cactcaaaac agcagtagca gcacttacta ttactactgt aaataaattt ttgagattgc
     2521 tattcataat tgttctttta aaatgattat tctgatttaa aattaatgat taaaatattt
     2581 caaataccat gcctttcgta tgcaagtaaa taaaccagta gaaatgtcag tacatttaat
     2641 acatcattta acattctttt acatagcatt taacaagttg atgtattttg agataagaat
     2701 ataaaattaa aaaagcaaaa gatgctttta gttcttttgc ttttaaaagc aggaaatatc
     2761 acccgccgtt gctaaaagta gtattgctac tttttattgt aatactaagg taataatgtt
     2821 ttgatattaa ttgctttttt tagttataat attatgcaca ttgcattgtg ctttaaatgt
     2881 gcattttatc tttcttggct aaaagttctt caactgtttc tctatacatt tcatctggca
     2941 cacagcaatc tacagggcaa actgctgcac attgtggttc ttcatgaaac ccttgacact
     3001 ctgtacattt atctggtaca ataaaatagg tgtccacact aattggttca ttacgttgtt
     3061 ctacatcaat ggtggtgcca tttaataatg taaaatctcc tttcactgta gtaccatctg
     3121 ttattgccca ctctactcct ccttcgtaaa ttgcattgtt aggacattca ggttcgcaag
     3181 ccccacagtt tatacattct tctgtaattt ttattgccat aataataaat tttatcttgg
     3241 ttatgaatag atagcaatag aaaagtattt tacaacctta cctttgccaa ctcaaaaata
     3301 aagttaagaa atgattttac aagaaagaat aaatttatta aaaaaattaa acaattattt
     3361 tcttgaaaat aatatagaac ttcaaaacgt aaaacaaaag gcatttcaag aaaatgcttg
     3421 gtttatacca gagtttgtgg aagaaagtat aaaaaatatt tcgaatgaat ttttagagga
     3481 gaacaaatta aataacttaa ttcaacaata taaaattcct gcagaaaata tacaacctaa
     3541 aagaattggt attgtaatgg ctggtaacat tgcattagtt ggttttcatg attttttatg
     3601 tgcatttata acaggacatt ctgttgttat taagccttct tctaaagatt ctgttttaat
     3661 aaaacatatt gttcaaaaat tagctgaatg gaatagcgaa attaatagca taataagctt
     3721 tgctgaaatt ttgaaaggtt gtgatgcgtt tatagcaaca ggcagcaaca acagcagtag
     3781 atactttgaa tattactttg aaaaataccc aagcattatt agaaaaaata gaactagtgt
     3841 tgccatactt actggcaaag aaagtaaaac cgaattagat ttactagcta aagacattca
     3901 actatatttt ggattaggtt gcagaaatgt tactaaactt tttgtacctg agaagtatga
     3961 ttttatacca ctattagatg cattaaaaaa atacgattat tatttagagt accacaaata
     4021 caaacataat tacgattacc atttggcatt attaataatg ggtaataaat tgtatatgaa
     4081 taatggctct attgttttta ctgaaaatga aagtaattac tctcctatta gtcaagttca
     4141 ttatagttac tataaaaaaa atgaaattga ttatttacta gaaaacattc aaccgaatca
     4201 agaaattcaa tgtataatag gctatcaagg tgtgccattt ggttgttctc aacaaccttg
     4261 tataaccgat tttgcagatg gagtggatac catacagttc ttaataaatt tgaactagaa
     4321 tttagggcaa agaggaacaa attttgagct attatctcta tcaaaaaaac ctgtatagga
     4381 aattgaaaac tccatagcac ctctaccctg gcttgcagtt cttaattgag aaacattagc
     4441 atcgtaacta aatgaaaatg caaaagggtg ataatctaat tttatcattg gtataaaagc
     4501 atctttagcc ctcataaatg cacctacatg aaaagtgtat ttaggattgt ctatatcatc
     4561 gccaatttta taagaataca ttgctccacc aattagctct gtaaagctac cttgtttaga
     4621 ataatcggca tgaattgtaa aataagaagt ttcatttagt gaaaatttga tacctgtaga
     4681 gacaaccatt ttggcttgta attctatatc ggggttttta tagaaagaat ttttaggacg
     4741 atttaaatgg tgatatgcaa cacctataaa ataattatcc tctgcttttc cgccaattgt
     4801 tgaattaaaa ctaagcccta tacttccatc taaataatta aaattagaat ttaaaaatgt
     4861 ttctccatct ggtaaagaag ggttataacc attaccatca taatggctat ttgtagtaat
     4921 cttacttcta tcgaattttc tttgtacaaa gcctcccatt atgccaagag aaaggtattt
     4981 atttttttct ccacttaatg ctttatggta atttaaagct ggtaaaaaac ttgtagttgt
     5041 taaagaggca gagcctgctt tatcatacaa catttgcata cctaaagtta aaaaatcatc
     5101 tgctccacca attggttttt tatattcaaa attaaaagag ccagttgtaa aaggagtagt
     5161 taccgatgcc cattgacttc tatatatacc ttgaaaacga atgtctcctt gaaataatcc
     5221 tgaaaaagaa ggatttctta aaaagggagc ttcaaaaaat tgtgaaaaat gaacatcttg
     5281 tgcttgtaca ttttgttgct tgccaaaaag cataaaaagc acaataattg tggtaaaatg
     5341 gatacggttt ctcattttta gagatattgg gtacaataca aacgtacttt ttattttatt
     5401 tattgtataa acccttttca taatagtgct tttttagcgt aaaagcgtaa tatttccttt
     5461 taaaaacatt gtttggctat ttgcacaaat aacttctata gtataaacgt aaacatctgc
     5521 agaaagttgt ttaccattaa atgttccatc ccagccattg gcagcattat tggcattaaa
     5581 atctctttta gtaaatacaa tttcgcccca acgattaaaa atggtcatat ttcttactgt
     5641 aaataaacct tttcctcttg gataaaaaac atcatttacg ccatcgttgt ttggtgaaaa
     5701 tgtatttgga ataaaaacat tgtatccatt acaaagtggt tcaatagtta cttcatctct
     5761 ggcaaagcaa ccaccaggat tagatgcttc ggctacatat ttaattttat ctgttataat
     5821 tgcattaggc gttgggcaat cggcacatgt aagccattga tttggtatcc attcccattt
     5881 agttatatct gcagagttgg aagttagtaa aggaaccaca ttgccaacat tagattgaac
     5941 taatgattca atgatattga attgaggcat ggggaaaacc ctaatctcaa ccaatcctgt
     6001 atcagcaaaa cactttttat tatcggttgc tataacagta taaacagtag ttgtagttgg
     6061 cgtagcaata gggttagcaa tatttggatt gtttaatcct gttgttggtt gccattgaaa
     6121 tttatctgca ttaccagatg ctaaaagttg agaactgcta ccagtacaaa tactatcatt
     6181 tgctgaaatt gaaaaggtaa atggttcaat tactgttact gccacagaat cttcattttt
     6241 acagccaaaa ctgcttgtac ctgttacttt ataaatagaa gagttttggg taaatacatt
     6301 tgggcttgca cagttagtac aacttaaagt agaatccatg ttccaaacat accctacacc
     6361 accacttgca tttagtttaa tagtttttcc cttacaaaca aaagtatcca caccagcatt
     6421 tattattggc aaagggtgta ttaaaatatt ttttgtaaca gtatcggcac agcctgctat
     6481 attaaatgca ataactttaa tattataagt gccagccgta atgtattgtt gtgtagtagg
     6541 gttctgaagt acagaggtat ttccatttgc caaattccat tgccaactga ttgcatttgt
     6601 atcacttttt attattgctc cattaaattg tagtaaacta ttaatgcaag aaacagaatc
     6661 tcctgtaata gctattgaag gtgtttgcac aacgttaata ggaacattca gtgtatcggt
     6721 attagtgcat cctaagttgg ttgttacttt tagagtaatt ttatagctac ctgtttgatt
     6781 gtaaaaatga gtagggtttt ttattgttga ggtaactcca tctccaaagt tccataaata
     6841 ggttgaaaca gtatcgttat aaacaatagt tgagtctaaa aaattaagtt gaacagaatc
     6901 gcagaaattt gtttgtggta cttttataag agtgcttaca tctgctactc ttactgtatc
     6961 tgttccaata attggcactt gacaccctgc tgaatctatc ataataattc caggcctgta
     7021 aataccaggt gtattgtagg tatgagaaat tgtagaatct ggtgttaatg taattacccc
     7081 atctgcaaag tcccaaacaa aggttgcgtt gttttttgta tgagcaacaa agtttacaac
     7141 agttggtgaa caaaactgta atggactgta agaaaatgta cctgttggcc ctcgtacaat
     7201 tatatctttt tgggttgaat caacacaacc cccatagcca taagctttta gttttaaagt
     7261 gtatttacct gcataagtat aaatatgaga aggtgtatct aaattagaaa agccattatc
     7321 accaaaactc caatttacac taaccgcatc agaagataaa ttagcagttt ctattaaaaa
     7381 aggataacac aaacccaaag tatctccttg taaaaattta aagttagcaa ccgaattggc
     7441 aaccctaata tacaatggtt ttaaaagtgt atctacacat ccaaaaatat cagtaacttt
     7501 taatttaacc gagtaaagcc cttgaagcaa atagggatgt tgagggtttt gttgtgttgc
     7561 agtttgagta tctccaaaat tccacaaata agtaaggtta actcctttag aagtgtcttg
     7621 aaatgttaca ttgctatttg cacatctaat agtatcgtta ctaaaaaaac ctgcaatagg
     7681 cttggttatt tgaaccgcat ttggttttat taaagtatca taacagccaa agtcatccaa
     7741 cacttttaat ttaacagtaa aaaaaccagt tgcattatat tgatgctggt atggtggaga
     7801 agttagtgta tctgttatac catcaccata attccaaatt acttgtgtta agttatgaat
     7861 accatctgtt gtagatgtat tggtgaaatt aattgtatca tataagcaag tacctggctg
     7921 atttacaaaa ttagcatttg gtccaaagat tttaattggt ggcgaaatag ttaatgtatc
     7981 tgcacaacca gcaacatcat ttataattac agttggagct acattgccat ttgtggtgta
     8041 tgcatgtgta actgaatcta tattggagcc actcattatt gtaccatcgc caaaatccca
     8101 cacataggaa gtaattaagg atggaactat gttatttatt ttatatgtag ctaaggcata
     8161 tttacaaaat gcagtatcag tagtaaccaa aattggatta ttaaatgaaa gattagcagg
     8221 tgcagttgca gaatgtgtac atacaccatt tgttacagta agcttaacat tataaatacc
     8281 ataagttgca taagtatgtg ttgggctttg tgcagttgat gtgttcccat ctccaaaatc
     8341 ccataagtaa gttaatccag ctcctttaga ttgatctata aaggttcttg ttaaaggagt
     8401 atcgcagcta aaaacagtat aaaacaaagc aataggtgga ttaatgtata tataattatt
     8461 tagttgcaat gtatcttta
//
LOCUS       DAOQHP010000047         8307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4386_length_8307_cov_3.771328, whole genome shotgun sequence.
ACCESSION   DAOQHP010000047 DAOQHP010000000
VERSION     DAOQHP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4386_length_8307_cov_3.771328"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLY81_02675"
     CDS             <1..916
                     /locus_tag="PLY81_02675"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNF29165.1"
                     /translation="LFKSFIQKSKSQTKFMMANPQVTFIDTFYKAMYKNNPLAPIAIP
                     KGEYYDKINLDRSLAIYKERFGDASGLHFVLVGSLPKDTTEMLIEKYIASLPVSNKKF
                     NFKDNGLRTAKGKININVHKGKEEKALILAVYSGEVPYSESLSLKAEAISEVLNIKII
                     EELREKIQGIYGGGIYSSVEKYPYNNYNYVAQLPCGPEKADTLVIALNNEINNIKTKG
                     PSVEDLNKVKQQWIEQYKTSIKENRTWLTKIQAITLEGASVDYFLNYEKIVNALTAAQ
                     IKATANLLFNGKNVITAVLKPEEKKGEE"
     gene            complement(995..2305)
                     /gene="aroA"
                     /locus_tag="PLY81_02680"
     CDS             complement(995..2305)
                     /gene="aroA"
                     /locus_tag="PLY81_02680"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNF29166.1"
                     /translation="MQAIIHPSQINGIITAPASKSIMQRACAAALIKKGKSIIYNYGN
                     SYDEKTALEIIQCLGATTIVNNNKICIESNGLYAVNNNIHCNESGLSVRLFTFLAATL
                     NEEITINANGSLLKRPMHFFKEIFPLLNVTIHQSNNIFPFFVKGPLLPTTIEINASIS
                     SQYLTGLLFAYSAALQNKYQTDSFLNEQICIKANNLNSKPYINLTLQVMKHFGLFTPE
                     VVGENRFCFNSKNYERSNANTTTEFTVEGDWSNAAFLLVAAAIAGKVKVNGMNFISEQ
                     ADRRIVEVVEKIGCNFFINNESIQVEKKHLNCFDFDATHCPDLFPPLVALACNCKGIS
                     TIKGVNRLLYKESNRAEALQTEFAKMGVEILVDGDIMIIKGDNEIKGSNLNSHQDHRI
                     VMACTIAALNASQNTKICNVEAVNKSYPLFFDHMKKLGTRIELY"
     gene            complement(2311..3321)
                     /gene="aroB"
                     /locus_tag="PLY81_02685"
     CDS             complement(2311..3321)
                     /gene="aroB"
                     /locus_tag="PLY81_02685"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNF29167.1"
                     /translation="MIKQTYLFKYSATNIFFNMPISSLTKLVDVKQCVIITDENIFAL
                     YKVKFKKYNTIVLKAGEEYKVQQTADSIIKQLLEFDADRNTILIGVGGGVITDITGYV
                     ASIYKRGVQLGFVPTTLLAMIDASIGGKNGIDVGHYKNMVGCFKQPNFILFDFLFLKT
                     LPQKQWQQAFAEIIKHACIKDAALFKEIQINNLQFYRSNKKALIALIEKNIVIKLGIV
                     QKDELENGERKLLNFGHTLAHALENLYQLNHGNAVSIGIAFAVKLSQKITGFNQTSAV
                     IELLNKYNLPVELNYKASKVFEVLKKDKKRNQQELHFVLLNKIGKAVIVPIPLQEIEK
                     LL"
     gene            complement(3378..4433)
                     /locus_tag="PLY81_02690"
     CDS             complement(3378..4433)
                     /locus_tag="PLY81_02690"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNF29168.1"
                     /translation="MFTKRPFIIAGPCSAETQEQLMQTAINLKSNTNIDLFRAGIWKP
                     RTRPGQFEGVGKVGLQWLQQVKNETGLATTIEVANTVQVDEALKHNIDVLWLGARTVA
                     NPFSVQEIATALQGVNIPILIKNPTNPDLELWTGAVERISKAGITNIGLVHRGFSVYG
                     NDEYRNAPMWHLAIEMKRRHPEKMFLCDPSHIAGNRNILQAISQKSIDLNYDGLMIES
                     HISPDNAWSDAKQQITPNILGSLLAKLIWRNEFAECNEDKNLLENLRQSINLLDDELI
                     QLLGKRMKIADTIGRFKKDNNITILQTSRWNEILLKIFSKGEELGLSKEFLTKYFDAV
                     HMESINHQNKVMNDDIQ"
     gene            complement(4474..6141)
                     /locus_tag="PLY81_02695"
     CDS             complement(4474..6141)
                     /locus_tag="PLY81_02695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF29169.1"
                     /translation="MITFSALGLDEKLVKATDALGFEHATPIQEKSIPVLLSGTKDMI
                     GLAQTGTGKTAAFGLPLLQLVQVTEKYPQALIVCPTRELCLQIVNEIEQFKKFISGMH
                     VVAVYGGASIGMQIRELKRGVQIVVATPGRLIDLIERKAINLEQIKYVVLDEADEMLN
                     MGFQEDIEFILQNTPQRESTWLFSATMPGEIKKVTKKYMKNPFEVTVGKVNTANKSID
                     HQYFITQAQHRYETLKRLIDFNPGMYGIIFTRTKADAQEIAEKLTREGYDIDALHGDL
                     SQPQRDKVMNAFRDKTLQLLIATDVAARGIDVKEITHVINYELPDDTEVYTHRSGRTG
                     RAGSTGVCMSIVHAREIYKLRNIERFIQTKFNKLEIPSGKDVCRKQFYTFMDKLLQTD
                     ISHGEYATFLPMLQEKFAEVSKEEVLQRVAALEFDRFLKYYENAEDLNIREQKRDTKR
                     EFGGDGRDQARSRGRDNKGVKYSGGNGFARLFVNLGTKDGFYKASFLQFILDMSDLRK
                     DVLGRIDMKEMNSWIEIDKKAAKQMIQSIDGKNYRGRKIRMNDANSR"
     gene            complement(6235..6732)
                     /locus_tag="PLY81_02700"
     CDS             complement(6235..6732)
                     /locus_tag="PLY81_02700"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNF29170.1"
                     /translation="MKKFFYLLVAALIFQACCVCNKMSAVDAMQFDKIVFHSSTCFGS
                     CPQIAIEINREGGTKLKRQFFNESATQTKERSGEFKGHISKEALDGLMLELNHSNYET
                     LQFPNVDCCDAPVITIIVYAKGKKVYLRSMMPPKDAGDLIKYLTELTTTVELPLAEDK
                     FEIEN"
     gene            6842..>8307
                     /locus_tag="PLY81_02705"
     CDS             6842..>8307
                     /locus_tag="PLY81_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNF29171.1"
                     /translation="MIRLLLVVGLLICNNIIAQTTNNSISIIPLPNNITIGKGYFTLN
                     NLTTINYTTAESKKLALLLQEYLQVQYNLNIKITEKATNNCIQFITNKKTNNEAYDLV
                     ISTNKIEIIGYDAGLFYGYQSLLQLLPVTIKNKEILLPIVTIKDTPRFEYRGMHLDVC
                     RHMFPLSFLKKYIDILASYKLNVFHWHLTDDQGWRLEIKKYPKLTSIGAYRNQTLIGN
                     YRTVGEYDNTAYGGFYTQEQAKELVAYARERFVTIIPEIEMPGHALAALAAYPTLGCG
                     DKTTNFQTAQTWGVFDDVFCAGKEETFELLQNVIDEVIEIFPSKYIHIGGDECPKTKW
                     KTCSSCQKRIKDNNLKDEHELQSYFIQRMEKYINNKGRKIIGWDEILEGGLAANATVM
                     SWRGDAGGIAAANQNHDVIMTPASYGLYFDHKQGADRNREPLSIGGLSTLEKVYLSNP
                     IPAVLEPTKHKFIKGVQANVWTEYIATTNKVEFTIYPR"
BASE COUNT         2769 a         1410 c         1133 g         2995 t
ORIGIN      
        1 tttatttaaa agttttattc aaaaaagtaa atcgcaaact aagtttatga tggctaaccc
       61 tcaggttact tttatagata ctttttataa agcaatgtat aaaaataatc cgttagctcc
      121 tatagctatt cctaaaggtg agtactacga taaaataaac cttgaccgtt cattagccat
      181 ttataaagaa cgttttggcg atgcaagtgg tttacatttt gttttagtag gtagcttacc
      241 aaaagatact acggaaatgc taattgaaaa atatattgct agccttcctg tttccaataa
      301 aaaattcaat tttaaagata atggcttacg tacagcaaaa ggaaaaatta atattaatgt
      361 acataaagga aaagaggaaa aagctttaat acttgctgtg tatagtggag aagtgcctta
      421 tagcgaatct ttatccttaa aagcagaagc tattagcgaa gttttaaata ttaaaattat
      481 tgaagagctg agagagaaaa ttcaaggtat ttatggtggt ggaatttaca gtagtgttga
      541 aaaatatcct tacaataatt acaactatgt tgctcaatta ccttgtggcc ctgaaaaagc
      601 agatacactt gtaattgctt taaacaatga gattaataat attaagacta aaggtccatc
      661 tgtagaagac ttgaacaaag taaaacaaca atggatagag caatacaaaa cttctataaa
      721 agagaacaga acttggttaa ctaaaattca agctattact ttagaaggtg ctagtgttga
      781 ttacttcctt aactatgaaa aaatagtaaa tgcattaaca gcagcacaaa ttaaagcaac
      841 tgcaaatctt ttatttaatg gtaaaaatgt aataactgca gttttaaaac cagaagaaaa
      901 gaaaggtgaa gaataattaa cttctttcac caacaaaata aagccgaatg aatcaaaaac
      961 attcggcttt atttttttaa aaatatacta tagcttaata aagttctatt ctagttccta
     1021 attttttcat gtggtcaaaa aacaatgggt aagatttgtt tacggcttct acattacaaa
     1081 tttttgtgtt ttgactagca tttagtgctg caattgtgca agccattaca attctatgat
     1141 cttgatgaga attaagattg gatcctttaa tttcattgtc acctttaata atcattatat
     1201 ctccatccac caatatttca actcccattt tagcaaattc agtttgcaaa gcttctgccc
     1261 tattactttc tttatatagt aacctattaa ctccttttat ggtagaaatt cctttacagt
     1321 tgcaagctag agccactaag ggtggaaata aatctggaca atgagttgca tcaaaatcaa
     1381 agcagtttaa atgttttttt tcaacttgaa tagattcatt atttataaaa aaattacacc
     1441 caattttttc tacaacttct actattcttc tatcggcttg ttccgatata aaattcatac
     1501 cattcacttt taccttgcca gcaatagcag cagctaccaa taaaaaagca gcattactcc
     1561 aatctccttc aactgtaaat tcagtggttg tgtttgcatt acttctttca taattcttac
     1621 tattaaagca aaacctgttc tctcctacca cttctggagt aaataagcca aaatgcttca
     1681 taacttgtaa ggtaaggttt atgtaaggct tactattaag gttatttgct tttatacaaa
     1741 tttgctcatt caaaaaacta tctgtttgat atttattttg taaagcagca ctataagcaa
     1801 ataataatcc agttaaatat tgagaactta tacttgcatt tatttcaata gtagtgggta
     1861 gtaatggtcc tttaacaaaa aatggaaata tgttattgct ttgatgaatg gtaacgttta
     1921 agagaggaaa aatttcttta aaaaaatgca taggtctttt taataaacta ccatttgcat
     1981 taatagtaat ttcctcattt aaagttgctg ctaaaaatgt aaatagcctt acacttaacc
     2041 cactttcgtt acaatgtata ttattattta ctgcataaag cccattgctt tcaatacaaa
     2101 ttttattgtt attaactatt gtagttgctc ctaagcattg aatgatttct aatgctgttt
     2161 tttcatcata gctgttacca taattataaa ttatgctttt acctttttta attagtgcag
     2221 cagcacaagc cctttgcata atacttttag atgcaggggc agtaataatg ccattaattt
     2281 gtgatgggtg aatgatagct tgcacaagta ttataataat ttttcaattt cttgtaaagg
     2341 aataggtaca ataacagctt tgccaatttt atttaataaa acaaaatgca actcttgttg
     2401 atttcttttt ttatccttct tcagtacttc aaaaactttt gatgccttgt aatttaattc
     2461 cacaggcaag ttatacttat tcaataattc aataactgca cttgtttgat taaatcctgt
     2521 aattttttgg gaaagtttaa ccgcaaatgc aatacctata gaaactgcat ttccatgatt
     2581 taattgataa aggttctcca atgcatgtgc taatgtatga ccaaaattta gcaatttcct
     2641 ttctccattt tctagttcat ctttttgaac aatgcctaat tttataacaa tatttttttc
     2701 tattaaagct attaatgctt ttttattact cctataaaat tgcaaattat ttatttgaat
     2761 ctctttaaat aatgcagcat cttttataca agcatgttta ataatttctg caaatgcttg
     2821 ctgccattgt ttttggggta aagttttcaa aaacaaaaaa tcaaataaaa taaaattggg
     2881 ttgtttaaaa cagccaacca tatttttata gtggccaaca tcaattccat tttttcctcc
     2941 aatactagca tctatcattg caagtaaagt agtaggtaca aaccctaatt gtacaccacg
     3001 tttataaatg ctggcaacat aacctgtaat atcagtaatt acaccaccac caacaccaat
     3061 taaaatagtg tttctatctg catcaaattc taataattgt ttaataatac tatcggctgt
     3121 ttgttgtact ttgtactcct ctcctgcttt taaaacaata gtgttatatt ttttgaattt
     3181 tactttatat aatgcaaaaa tattttcatc ggtaatgatt acacattgtt taacatccac
     3241 caatttagtt aaactgctaa ttggcatatt aaagaagatg ttggtagcag aatatttaaa
     3301 tagataggtt tgtttaatca tacagcttac ttgataaaaa ataatggttt aaagttaatg
     3361 tttaaaaccg tgccatgcta ctgtatatca tcattcatta ctttattttg atgattgata
     3421 ctttccatat gaactgcatc aaaatatttc gttaagaact ctttacttaa ccccaactcc
     3481 tctccttttg aaaatatttt taagagtatt tcattccatc tgctggtttg taaaattgta
     3541 atattattat cttttttaaa tctccctatt gtatcggcaa tcttcattct tttccctagt
     3601 aattgtataa gttcatcatc taataggtta atgctctgtc ttaaattttc taacaagttc
     3661 ttgtcttcat tacattctgc aaattcattt ctccaaatta atttagcaag taaacttcct
     3721 aatatatttg gtgtaatttg ctgtttagca tcactccaag cattatcagg agaaatatgg
     3781 ctttctatca ttaagccatc ataattaagg tcaatacttt tttgtgaaat ggcttgtaaa
     3841 atatttctat tgccagcaat gtgagaagga tcgcatagaa acattttttc tggatgcctt
     3901 ctcttcattt ctattgccaa atgccacata ggagcatttc tgtactcatc atttccatac
     3961 acactaaaac ctctatgcac taaaccaata tttgttatgc cagccttact aattctttcc
     4021 actgcaccag tccaaagttc taaatcgggg tttgttggat ttttaattaa aataggaata
     4081 tttacacctt gtaaagcagt agcaatttct tgtacactaa aagggttagc aacagttctg
     4141 gcaccaagcc aaagtacatc aatattatgt tttaaagcct catcaacctg tacagtattt
     4201 gccacctcta ttgtagtagc taagcctgtt tcatttttaa cttgttgtag ccattgtaaa
     4261 ccaaccttgc caactccttc aaactgccct ggccttgttc ttggcttcca aattcctgct
     4321 ctaaataaat caatattagt attgcttttt aaattaatgg cagtttgcat tagctgctcc
     4381 tgtgtttctg cagaacatgg cccagcgata ataaaaggtc gttttgtaaa catagtttaa
     4441 agcttgttta cctttaaatt ttaagcaaaa atattaacga gaattagcat cattcattct
     4501 aatttttctt cctctatagt ttttaccatc tatagattgt atcatttgtt tagccgcttt
     4561 tttatcaatt tcaatccagc tattcatttc tttcatatct attcttccta aaacatcttt
     4621 tcttaaatca ctcatatcta aaataaactg taaaaagcta gctttataaa atccatcttt
     4681 tgtacccagg ttaacaaata gtctagcaaa accattacca ccactatatt tcactccttt
     4741 attgtctctt cctctgcttc ttgcctggtc tcttccatcg cctccaaatt ctcttttagt
     4801 gtctcttttc tgttctctaa tgtttaaatc ttctgcattt tcataatatt tcaaaaacct
     4861 gtcaaactct aaagcagcta cccgttgcaa tacttcttct ttagatactt cggcaaattt
     4921 ttcttgtaac attggtaaaa atgtagcata ctctccatgc gaaatatctg tttgtaatag
     4981 tttatccatg aaagtgtaaa actgttttct acaaacatct ttaccacttg gaatttctag
     5041 tttattaaat tttgtttgaa taaaacgttc aatatttcta agcttataaa tttctctagc
     5101 atgtacaatg ctcatacaaa cgcctgtgct gcctgcacgt cctgttcttc cacttctatg
     5161 tgtgtaaact tcagtatcat caggtaattc gtagttaatt acatgggtaa tttctttaac
     5221 atctatacct cttgcagcta catctgttgc tataagtaat tgtagggttt tatctctaaa
     5281 agcattcata actttatctc tttgtggttg agataagtct ccatgtaagg catcaatatc
     5341 atatccttct ctagttaatt tttctgcaat ttcctgagca tctgcttttg ttcttgtaaa
     5401 aataattcca tacatacccg gattaaaatc tatcaacctt tttaaggttt cgtatctatg
     5461 ctgtgcttgt gtaataaaat attgatgatc tatactctta tttgctgtat ttactttgcc
     5521 aactgtaact tcaaaaggat ttttcatgta ctttttggtt acttttttta tctcgccagg
     5581 catagtagca ctaaatagcc aagtactttc tctttgaggt gtattttgca aaataaattc
     5641 aatatcttct tgaaagccca tgtttagcat ctcatctgcc tcatctaaaa ctacatattt
     5701 tatttgttct aaattaattg cttttctttc aattaaatca attaacctac ctggtgttgc
     5761 aactacaatt tgcaccccac gttttagttc tcttatttgc atgccaatac ttgctccacc
     5821 ataaacagca acaacatgca tacccgaaat gaatttttta aattgctcta tttcgtttac
     5881 aatttgtaaa caaagttctc tggtagggca aactattaaa gcctgaggat atttttctgt
     5941 tacttgtact aattgtaata atggcaaacc aaaagcagcc gttttacctg ttcctgtttg
     6001 tgccaaacct atcatatctt tagttccact taacaataca ggaatacttt tttcttgaat
     6061 aggtgtagca tgttcaaagc ctaaagcatc ggttgcctta accaattttt catccaaacc
     6121 caacgcacta aatgttatca tttattttaa aaatttgcgg caaaggtaac taatagcatt
     6181 tactatgcta attttttgtg ttcatcagta aataattgtg cagtaaacag gcttttaatt
     6241 ttcaatttca aatttatctt cagccaatgg cagttcaaca gttgtagtta actctgttaa
     6301 atatttaatt aagtcgccag catcttttgg tggcatcatt gaacgcaaat acactttttt
     6361 gcctttagca taaactataa ttgtaattac tggtgcatcg caacaatcta catttggaaa
     6421 ttgaagagtt tcatagttac tatggtttaa ctcaagcatt aatccatcta acgcttcttt
     6481 acttatatgt cctttaaact ctccagaacg ttctttagtt tgagttgcac tttcattaaa
     6541 aaattgtctt tttaactttg ttcctccttc tctatttatt tctattgcaa tttgtgggca
     6601 actaccaaag caagtggagg aatgaaatac tattttatca aattgcatcg catcaacagc
     6661 actcatttta ttgcatacac aacatgcttg aaaaattaat gcagcaacta ataaataaaa
     6721 aaactttttc atactataaa attactttat gcaaatttta aataaaagta gttagattaa
     6781 tttcattctt taaatttatt cttcgtttta atcaaatatt tttgacacta aaatatatac
     6841 tatgataagg ctattgctgg tagttggttt attaatttgc aataatatta ttgcacaaac
     6901 tactaataat tctatttcca ttattccatt acccaataat attacaatag gtaaaggata
     6961 ttttacttta aataacctaa caacaataaa ctatacaact gccgaaagca aaaaactggc
     7021 tttattgtta caagagtact tacaagttca atataacttg aatattaaaa taactgaaaa
     7081 agctacaaat aattgtattc aatttataac aaataaaaaa actaataatg aagcttatga
     7141 tttagttatt tcaaccaata aaattgaaat tattggttat gatgcaggtc tgttttatgg
     7201 ataccaatct ctacttcaac ttttaccagt aaccataaaa aataaagaaa tattattacc
     7261 tattgttacc ataaaagata caccaagatt tgagtataga ggaatgcatt tagatgtttg
     7321 caggcatatg tttccactaa gttttttaaa aaaatatata gatatattag caagttataa
     7381 actaaatgtt tttcattggc atttaaccga cgatcaaggt tggcgtttag aaataaaaaa
     7441 atatccaaag cttacctcta ttggtgcata tagaaatcaa actttaattg gaaattaccg
     7501 aacagttggt gaatatgaca atactgctta tggtggattt tatacacaag aacaggcaaa
     7561 agaattggtt gcttatgcaa gagaacgctt tgttactatt attcccgaaa ttgaaatgcc
     7621 tggccatgca ttagctgctt tggctgctta tccaacattg ggttgtggcg ataaaacaac
     7681 taactttcaa acagcacaaa cttggggcgt ttttgatgat gttttttgtg ccggcaaaga
     7741 agaaacattt gaactactgc aaaatgttat tgacgaagta attgaaattt ttccaagtaa
     7801 atacattcat attggaggcg atgaatgccc taaaacaaaa tggaaaacct gtagctcatg
     7861 tcaaaaaaga attaaagaca ataacttaaa agatgaacat gaattgcaga gttatttcat
     7921 tcaaagaatg gagaaataca ttaataataa aggtagaaaa attattggat gggatgaaat
     7981 acttgaaggt ggattagcag ccaatgctac agtaatgagt tggcgtggag atgcaggagg
     8041 aattgcagca gcaaatcaaa atcatgatgt aattatgaca cctgcatcgt atgggttata
     8101 ttttgatcat aaacaaggag cagacagaaa tagagagcct ttaagtattg gcggcctttc
     8161 tactttagaa aaagtatatc tatcaaaccc tatacctgca gttttagaac caacaaaaca
     8221 taaatttatt aaaggcgttc aagccaatgt ttggacagaa tatattgcaa ctacaaacaa
     8281 ggtagagttt accatttacc caagaat
//
LOCUS       DAOQHP010000048         8292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4394_length_8292_cov_3.737404, whole genome shotgun sequence.
ACCESSION   DAOQHP010000048 DAOQHP010000000
VERSION     DAOQHP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4394_length_8292_cov_3.737404"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..551
                     /locus_tag="PLY81_02710"
     CDS             102..551
                     /locus_tag="PLY81_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNF29172.1"
                     /translation="MIKINYPTYQFKFNQKNGKEFIFDAFRKLWVRLTPEEWVRQNFL
                     QYLVQEKKYPIACIAVEKEVMVNGIKKRCDIIVFKNSAPYMIVECKEMNVALTEKTIQ
                     QILTYNQSLSVNILVITNGNNTFALETTTKKSLNSIPAFSETIINDF"
     gene            597..2273
                     /gene="hutU"
                     /locus_tag="PLY81_02715"
     CDS             597..2273
                     /gene="hutU"
                     /locus_tag="PLY81_02715"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNF29173.1"
                     /translation="MSTYQRYDENKYKTPTGSSLNCKGWIQEAALRMLLNNLNPEVAE
                     RPDDLIVYGGRGKAARNFESLDLIIKALKNLENNETLLIQSGKPVAVLTTHADAPRVL
                     ISNSQLVPNWANWKHFTELEQKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAAAADKQ
                     FGGTLKGTLNVTAGLGGMGGAQPLAITMNEGVALVAEVEEWRIDKRIETKYLDEKYTD
                     IDKAIDAALEYKKQSVAKSIGVLCNAVHLLQKLNERNIIPDTLTDQTSAHDPLIGYVP
                     HTLTNEQANELREKNAEEYIKLSYESMYLHVQLMLQLQQKGAITFDYGNNIRARAQEH
                     GLKNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPSDIAVTDELIMQMFPDNKGLMR
                     WMKMAKEKIAFQGLPARICWLGQGEREKAGLAFNELVRTGKVKAPIVIGRDHLDTGSV
                     ASPNRETEAMLDGSDAVADWPILNALVNTAGGASWVSLHHGGGVGMGYSIHAGMVIVA
                     DGTEEATKRLSRVLRNDPGMGVIRHADAGYELAKQTAQEHGLDLVAKQNS"
     gene            2282..2695
                     /locus_tag="PLY81_02720"
     CDS             2282..2695
                     /locus_tag="PLY81_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004869 - cysteine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin domain-containing protein"
                     /protein_id="HNF29174.1"
                     /translation="MPYYNTEESIQHVVTLLNKRLYNAGYSFRVIEVSEYTVYVQASF
                     DFGYYVNVHIQFDNCIFTNLKERDEWPDAWHADQLFLLDEVEVENILNWYDVEKEPTN
                     NLFAFIFNINGCNINNAGVVVCEKLYIRWEHPYYD"
     gene            2775..2987
                     /locus_tag="PLY81_02725"
     CDS             2775..2987
                     /locus_tag="PLY81_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29175.1"
                     /translation="MEQQHKLAEEQNKQDFTKSWVSSSRFLFYLLITCMMASTIGGCF
                     KLYKSKYKGTPEYEIQGSTKYTPDYK"
     gene            3060..3132
                     /locus_tag="PLY81_02730"
     tRNA            3060..3132
                     /locus_tag="PLY81_02730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3093..3095,aa:Gly,seq:gcc)
     gene            3164..3249
                     /locus_tag="PLY81_02735"
     tRNA            3164..3249
                     /locus_tag="PLY81_02735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3198..3200,aa:Leu,seq:taa)
     gene            complement(3298..3957)
                     /locus_tag="PLY81_02740"
     CDS             complement(3298..3957)
                     /locus_tag="PLY81_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNF29176.1"
                     /translation="MPELKQIISLIKKDILLEVRQQYSFYGIILYVASTIFVIYLTMG
                     QPEEQIWNGLFWIVQLFIVTNAVAKSFLSESKGKMIYYYTIAGAKVFILSKLIFNLLL
                     MLLMSLISLLLFMVLLNNPMHNAMLFVGITILGGTSLSLVFTFLSAIAAKAMQQAALM
                     AILGFPLIIPQLLLLMKITTPAFSNVIQESWGQLILMLIALDVMVVLLAIILFPFLWK
                     D"
     gene            complement(3973..4536)
                     /locus_tag="PLY81_02745"
     CDS             complement(3973..4536)
                     /locus_tag="PLY81_02745"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNF29177.1"
                     /translation="MFIEPNLSGERRGWIEVICGSMFSGKTEELIRRLKRAKIANLKV
                     EIFKPGIDVRYDETKVVSHDANAIQSTPVDNSQKILLLTQDVDVVGIDEAQFFDDEIT
                     NVCETLATKGIRVIVAGLDMDYLGKPFGQMPNLLAIADYITKLHAICVQCGNIANISY
                     RKIEQDGQVLLGEKDVYEPRCRVCYHK"
     gene            4623..5318
                     /locus_tag="PLY81_02750"
     CDS             4623..5318
                     /locus_tag="PLY81_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29178.1"
                     /translation="MERVGTLINKLKEQLDQKQDAAKLLLTAQMLLAELQQQQPSSNS
                     GKVSVTMPSVTFVSEIIAEVKNESKPKAVEQPKKAPQNENQTGWLFNPELASIPTLVH
                     QEIVTNEVVDKVEDKKELFELKDTLVTEEEPSLNDILKEEKTEIATVLKGAPIKDLKK
                     AIGINDRYVFINELFRGDEVMYERSIKTINTFNIYAEAEYWIQRELKVKLGWEENNST
                     VESFNQLVKRRFA"
     gene            complement(5291..6550)
                     /locus_tag="PLY81_02755"
     CDS             complement(5291..6550)
                     /locus_tag="PLY81_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNF29179.1"
                     /translation="MKIFYNIFIAVYPFIAKIISTKNTKAKLWLTGRKNIFTKLKEAF
                     ANNKSEVIWMHCSSLGEFEQGRPVLEKIKQTYPSYTILLTFFSPSGYEIQKNYAYANW
                     VYYLPLDSAKNATQFLDIVQPKLTLFIKYEFWYYYLTEIKKRNIPLLLLSASFRKNQP
                     FFKWYGKIYREMLQCFSYFFVQDNNCADLLHKIGFTKNVSIAGDTRFDRVIEIANSFE
                     SIEIIEKFIGTDKVIVAGSTWTDDDEALDHYANTNTHIKFIIAPHQIETERVNECLKL
                     YKKAILFSVLVNEPNSNFTEKNVLIIDNIGMLSKLYKYATICFVGGAFGDDGVHNVLE
                     AAVYKKPVVFGPEFEKYIEAKELVENNGGISIENALELEQVFNKLLTDNLFYETTAIN
                     AENYVQNNSGATKIIVDFIYANRLLTN"
     gene            6674..7807
                     /locus_tag="PLY81_02760"
     CDS             6674..7807
                     /locus_tag="PLY81_02760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF29180.1"
                     /translation="MKPIQMVDTKTQYLKIKEEVDKGIFEVLESAQYIGGAKVNEFSE
                     ALNVYHNSAFTITCANGTDALQIALMALDLQPGDEVITPSFTYIATTEVVALLRLKPV
                     FVEVNPKTFCIEPDAIRNAITPKTKAIVPVHLYGHAAPMEEIMQIAKEYNLYVVEDNA
                     QAIGCNYTFNNGTTKKTATIGHIGCTSFYPSKNLGAYGDGGAIFTQDAMLAEKLKMIA
                     NHGQKTRYIHDIVGCNSRLDAIQAAILTVKLKQLDNYIVARNNAANYYNKAFANHTKI
                     TTPFVASYSNHVYHQYTLILDGVNRDGLVNYLAEKKIPSMIYYPIPGHKQKVFETIGS
                     EKYNLEITDWLTEKVISLPIHTELETDQLEYITTEVLNYINNH"
     gene            7815..>8292
                     /locus_tag="PLY81_02765"
     CDS             7815..>8292
                     /locus_tag="PLY81_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNF29181.1"
                     /translation="MKIAVVGTGYVGLVSGTCFAETGNTVTCIDIDQNKVSKLSNGEI
                     TIYEPGLEKIFLRNLKENRLKFTTNLAEGIKDAAIVFLALPTPPGEDGSADLKYVLGV
                     ANDLGKIITDYKVIVDKSTVPVGTAAKVHAAIAANAKVEFDVVSNPEFLREGVAVDD"
BASE COUNT         3006 a         1229 c         1388 g         2669 t
ORIGIN      
        1 ttattcttct aagttttatt tttcttcttt taaattatgt accagccaac ttatatttgg
       61 caaatagcat ttgtaaatat aggcaagaat ttccttaaac gatgataaaa ataaattacc
      121 ccacctatca atttaaattc aaccaaaaaa atgggaagga gtttattttt gatgctttta
      181 gaaaactttg ggtaaggctt acaccagaag aatgggttag acaaaatttt ttgcaatacc
      241 ttgttcaaga aaaaaaatac cctatagcat gtattgctgt tgaaaaagaa gtgatggtaa
      301 atggcataaa aaaacgatgc gatattatag ttttcaaaaa ctctgcacct tacatgattg
      361 ttgaatgtaa agaaatgaat gttgccttaa ccgaaaaaac aattcagcaa atacttactt
      421 ataaccaatc attatctgta aatatattgg taataaccaa tggcaataat acatttgcat
      481 tagaaaccac aacaaaaaaa tcacttaatt caattccagc attttctgaa acaataataa
      541 atgatttttg aagttggctt ctttaacctc ttaacaaaaa ttaataatta acttttatga
      601 gtacttatca acgttatgat gaaaataaat acaaaacacc aacaggtagt agcttaaact
      661 gcaaaggatg gatacaggaa gcagcgttaa gaatgttgtt aaataattta aaccccgagg
      721 ttgctgaaag accagatgat ttaattgttt atggaggaag gggcaaagct gccagaaatt
      781 ttgagtcgtt agatttaatt ataaaagcat taaaaaattt agaaaataac gaaacattac
      841 ttatacaaag tggtaagcct gtagctgtac ttaccacaca tgcagatgca ccacgtgttt
      901 taatcagcaa ttctcaatta gtacccaatt gggcaaattg gaagcacttt actgaattag
      961 aacaaaaagg attaatgatg tatgggcaaa tgactgctgg tagttggatt tatataggaa
     1021 gccaaggtat tgtacaaggc acttatgaga cttatgcagc agctgctgat aaacagtttg
     1081 gaggaacact taaaggaact ttaaatgtta cagcaggttt aggaggaatg ggaggtgcac
     1141 agcctttagc cattacaatg aatgagggtg ttgctttagt tgccgaagta gaagagtgga
     1201 gaatagataa aagaattgaa acgaaatatt tagacgaaaa atatacagat atagataaag
     1261 caatagatgc agcacttgaa tataaaaaac aaagtgtagc aaaaagtatt ggcgtacttt
     1321 gtaatgctgt tcatttgtta caaaaattaa atgaaagaaa tattataccc gatactttaa
     1381 cagaccaaac atctgcccat gatccattaa taggttatgt gccacatact ttaacaaacg
     1441 aacaagcaaa tgaattaaga gaaaaaaatg cagaggagta tattaaactt agctacgaaa
     1501 gtatgtattt acatgtacaa ctaatgttgc aattacaaca aaaaggtgca ataacatttg
     1561 attatggtaa caatataaga gcaagagctc aagagcatgg tttaaaaaat gcctttgatt
     1621 ttcctggatt tgtgccagct tatatacgtc cattattttg tgaaggaaaa gggcctttta
     1681 gatgggctgc tttaagtgga gacccatcag atattgcagt aaccgatgaa ttaattatgc
     1741 aaatgtttcc tgataacaaa ggattaatgc gttggatgaa aatggcaaaa gaaaaaattg
     1801 catttcaagg attacctgca agaatttgtt ggttaggaca gggcgaaaga gaaaaagcag
     1861 gattggcatt taatgaactt gttagaacag gaaaagtaaa agcacccatt gttattggta
     1921 gagaccattt agatacaggt agtgttgcta gccctaacag agaaactgaa gcaatgttag
     1981 atggaagtga tgctgtagca gattggccaa tattaaatgc attggtaaat actgctggtg
     2041 gtgctagttg ggtaagttta catcatggtg gtggggttgg tatgggttat agtattcatg
     2101 caggtatggt aattgttgct gatggaacag aggaagcaac aaaaagatta agtagagttt
     2161 taagaaatga tccaggaatg ggagtaatta gacatgcaga tgctggttac gaattagcaa
     2221 aacaaactgc tcaagaacat gggttggatt tagttgcaaa acaaaacagc taacctactt
     2281 aatgccttac tataatacag aagaaagcat ccaacatgta gtaacattat taaacaaacg
     2341 tttgtataat gcaggatata gctttagggt tattgaagtt tcagaatata cggtttatgt
     2401 acaggcaagt tttgattttg gatattatgt aaatgtgcat atacaatttg ataactgcat
     2461 ttttactaat ttaaaagaaa gagatgagtg gccagatgct tggcatgcag atcagctttt
     2521 tcttttggat gaagtggaag tggaaaatat attgaactgg tatgatgtag aaaaggagcc
     2581 tacaaacaat ttatttgctt ttatttttaa tataaatgga tgtaatatta acaatgctgg
     2641 cgtagttgtt tgcgaaaaat tatatattcg ttgggaacat ccttattatg actgataata
     2701 gaaaaataaa tagcttagtt agatattgaa gttttaattt acatccttat ttttgcaaac
     2761 gttaaatacc aattatggaa caacaacaca aactagctga agagcaaaat aaacaagact
     2821 ttacaaagag ttgggttagt tcatctcgtt ttttatttta tttattaatc acttgtatga
     2881 tggcctctac aataggtggc tgttttaaac tatataaaag taaatataag ggtacaccag
     2941 agtatgaaat acaaggcagt actaaataca ctccagatta taaataaatt ttaaaaaaaa
     3001 ttttgtttag aactttggtt atatattttt gcacccccaa ttagttcttt aaacatactg
     3061 cggaagtagc tcatttggta gagcacaacc ttgccaaggt tggggtggcc ggttcgagcc
     3121 cggtcttccg ctctaaattt tgatttttcg aaattttaag gttgccctgg tggtggaatt
     3181 ggtagacacg caggacttaa aatcctgtgg ccagcaatgg ccgtaacggt tcaagtccgt
     3241 tccggggcac taaaaggttg tttcattttt atgattcaac ctttttttat ttataagcta
     3301 atctttccag agaaagggaa ataatataat tgctaataaa acaaccatta catctaatgc
     3361 tatcaacata agtattaact gcccccaact ttcttgtata acatttgaaa aagctggtgt
     3421 tgttattttc attaataaaa gtaattgtgg aataattaat ggaaaaccta agatagccat
     3481 taatgcagcc tgctgcattg ctttagcagc tattgctgat aaaaaagtaa atactaaact
     3541 taaacttgta cctcctagta ttgtaatgcc tacaaaaagc attgcattat gcattgggtt
     3601 atttaataaa accataaaaa gcaataagct aattaggctc attaaaagca ttagtaaaag
     3661 gttaaaaatt aatttactta aaataaaaac cttagcaccg gcaatggtat aatagtatat
     3721 cattttaccc ttactttccg ataaaaaact ttttgctaca gcatttgtaa caataaataa
     3781 ttgtacaatc caaaataacc cattccatat ttgttcttcg ggctggccca tagttaaata
     3841 aataacaaat atggtactag ccacatacaa aataattccg taaaaagagt attgttgcct
     3901 tacttctagc aaaatatctt ttttaatgag actaataatt tgtttcaatt caggcattaa
     3961 aaaatgagtt ttttatttat gataacaaac cctgcatctt ggctcatata catctttttc
     4021 tcctaataaa acctgcccat cttgttctat ttttctgtag gaaatattag caatattacc
     4081 acattgcaca catattgcat gcaacttagt aatataatct gctatggcta aaagattagg
     4141 catctggcca aatggtttgc caagataatc catatctaaa cctgcaacaa ttacacgtat
     4201 tccttttgtg gcaagtgttt cgcaaacatt cgttatttca tcatcaaaaa attgtgcttc
     4261 atcaattcct actacatcta catcttgcgt taacaataaa attttttgag aattatctac
     4321 aggtgtactt tgtattgcat tagcatcgtg gcttacaact tttgtttcat catatcttac
     4381 atctattcct ggtttaaaaa tttctacttt taagttagca attttggcac gttttaatct
     4441 tctaatgagt tcttctgttt ttccactaaa catactgcca catatcactt ctatccatcc
     4501 tcttctttct ccacttaaat taggttctat aaacatttta tttaacttgg gtttattagt
     4561 aaaagcgtat aactttattg cctaaatatt actatttaga aacctcaaat gtatactatc
     4621 caatggaaag agttggcaca cttatcaaca aattaaaaga gcaactagac caaaagcaag
     4681 atgcagctaa attattgctt actgcacaaa tgcttttagc cgaattgcag caacaacagc
     4741 ctagttcaaa tagtggaaaa gtatctgtta caatgcctag tgttactttt gttagtgaaa
     4801 ttattgcaga ggtgaaaaat gaaagtaaac caaaagctgt agaacaacct aagaaggctc
     4861 cacaaaatga aaaccaaaca gggtggttgt ttaatcctga attagcttct atacctacac
     4921 ttgttcatca agaaattgta acaaatgaag tagttgataa agtggaggat aaaaaagaat
     4981 tgtttgaatt aaaagataca cttgtaacag aggaggagcc aagccttaat gatattttaa
     5041 aagaggaaaa aaccgaaata gcaaccgtat taaaaggagc acccattaaa gacttaaaaa
     5101 aagctattgg tattaacgac agatatgttt ttatcaacga gctttttagg ggagatgaag
     5161 ttatgtacga acgtagtatt aaaaccatta atacttttaa tatttatgcc gaggcagaat
     5221 attggataca aagagaatta aaggtgaagc ttggatggga agaaaataat tctaccgtag
     5281 aaagttttaa tcaattagtt aaaaggcggt ttgcataaat aaaatctaca ataatttttg
     5341 ttgccccact attattttga acataatttt ctgcattaat tgcagtagtt tcataaaaca
     5401 aattatctgt taatagctta ttaaatactt gctcaagttc taaagcattt tctatactta
     5461 ttccaccatt gttttctact aattcttttg cttctatata tttttcaaat tctggcccaa
     5521 atactacagg ttttttatat actgctgctt ctaaaacatt gtgtacacca tcgtctccaa
     5581 atgcaccacc tacaaagcat attgttgcat atttgtataa cttgcttaac atgccaatat
     5641 tatcaattat taaaacattt ttttctgtaa aattgctatt tggctcgtta actaatacag
     5701 aaaaaagtat agctttttta tacaacttta aacattcatt aaccctttca gtttctattt
     5761 ggtgcggtgc aataataaat tttatgtgag tattagtgtt ggcataatgg tctaaagctt
     5821 catcatcatc agtccaggtg ctacctgcaa caataacctt atcagttcca ataaattttt
     5881 caataatttc aatactttca aaactgtttg ctatttcaat aactctatca aatctagtat
     5941 cgccagcaat agaaacattt tttgtaaagc ctattttatg tagtaaatca gcacaattat
     6001 tatcttgaac aaaaaagtaa gaaaagcact gcagcatttc tctatatatt tttccgtacc
     6061 acttaaagaa aggttgattt tttctaaaac ttgcggataa tagtagcaag ggtatatttc
     6121 ttttcttaat ttctgttaaa taataatacc aaaactcata tttgatgaat aaagtaagtt
     6181 taggttgtac aatatctaaa aattgggtag cattcttagc cgaatctaat ggcaagtaat
     6241 aaacccaatt agcataagca taattctttt gaatttcata acccgatgga gaaaaaaaag
     6301 ttaataaaat tgtataggat ggatacgttt gttttatttt ttctaaaacc ggccttcctt
     6361 gctcaaactc acctaagcta gagcaatgca tccaaattac ttcgctttta ttattggcaa
     6421 atgcttcttt tagtttagta aaaatatttt ttctaccagt aagccacaac ttggcttttg
     6481 tatttttggt acttataatt tttgcaatga aaggataaac tgcaataaat atgttgtaaa
     6541 aaattttcaa tagattgttt gttggtacaa agaaaataaa atagcttaaa ataaactatt
     6601 aagttaaaga gttttgctaa caaattaaac gtattatttt ttctaatacc tttgcccaca
     6661 tttattttta actatgaagc ctatacaaat ggtagatact aaaacacagt atctaaaaat
     6721 taaagaagag gtagataaag gaatttttga agtattagaa agtgcacaat atattggagg
     6781 tgcaaaagtg aatgaatttt ctgaagcatt aaatgtttac cataatagtg cttttacaat
     6841 tacttgtgct aatggtacag atgctttaca aatagcatta atggcattag acttacagcc
     6901 tggcgatgaa gtaattacac cttcttttac ttatattgct actaccgagg tggttgcttt
     6961 actgcgttta aaacctgttt ttgtagaagt aaatccaaaa actttttgca tagaaccaga
     7021 tgctataaga aatgcaatta cacctaaaac aaaggccatt gttcctgtac atttatatgg
     7081 gcatgcagca ccaatggaag aaattatgca aattgcaaaa gaatataatt tgtatgtagt
     7141 agaagataat gctcaggcta ttggttgtaa ttatactttt aataatggca ctacaaaaaa
     7201 aactgctaca attggccata ttggttgtac atctttttat ccaagtaaaa acttaggagc
     7261 ttatggcgat ggtggtgcaa tttttacaca agatgcaatg cttgcagaaa aattaaaaat
     7321 gattgccaac catggtcaaa aaacaagata cattcatgat attgttggtt gcaactcaag
     7381 gttagatgct atacaagctg caatattaac cgttaaatta aaacagttag ataattatat
     7441 tgttgcccga aataatgctg ctaattatta caataaagca tttgcaaatc atacaaaaat
     7501 tactactcct tttgttgcct cttacagtaa ccatgtgtat catcaatata ctttaatact
     7561 tgatggagta aatcgagatg gcttagtaaa ttacttggca gagaaaaaaa ttccatctat
     7621 gatttattat cctattcctg gtcataaaca aaaagttttt gaaaccattg gtagcgaaaa
     7681 atataactta gaaattacag attggttaac agaaaaagta attagtttac caattcatac
     7741 agagcttgaa acagatcaat tagaatatat aactacagaa gttttaaatt atataaacaa
     7801 ccactaaata aaatatgaaa atagcagttg taggcacagg atatgtaggc ttagtatcag
     7861 gaacatgttt tgcagaaaca ggtaatacag ttacttgtat tgatattgac caaaataaag
     7921 tatctaaact tagtaatggc gaaataacca tttatgaacc agggttagaa aaaatatttc
     7981 taagaaattt aaaagaaaat cgtttaaaat ttactaccaa tttagctgag ggtattaaag
     8041 atgcagccat tgttttttta gcattaccaa caccacctgg cgaagatgga agtgcagatt
     8101 taaagtatgt gttgggagtg gccaatgact taggaaaaat tataacagac tataaagtaa
     8161 ttgttgataa aagtactgta ccagtaggta cagcagcaaa agtacatgct gcaattgctg
     8221 ccaatgcaaa agttgaattt gatgtggtaa gtaatcctga gtttttaaga gaaggtgttg
     8281 ctgtagatga tt
//
LOCUS       DAOQHP010000049         8267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4415_length_8267_cov_3.489406, whole genome shotgun sequence.
ACCESSION   DAOQHP010000049 DAOQHP010000000
VERSION     DAOQHP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_8267_cov_3.489406"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PLY81_02770"
     CDS             complement(<1..1447)
                     /locus_tag="PLY81_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNF29182.1"
                     /translation="MLKFLKFAIILLMAVPISVMAQKQKNKSAKTNGSAVVDTTITDD
                     VKDAMSDNIAVVTIDENDQGDGGAQNVSSVLTAGRDPFFSVMSFNFNAFRFKMRGYDG
                     DLNTTYLNGTPMDNLDNGFTPFGLWGGLNDVMRNRDVSLGLRPSTFAFGDIASSSSID
                     ARASKQRKQTQIGYAISNRNYTHRLTVYHGTGISKKGWAFVFAGSFRGADEAYVPGTY
                     YNGYSYFIGVDKRFGQKHLLSLSLFEAPTESGRQSGVVDELRTITGDNFYNPTWGLQN
                     GKKRNANIAKSSQPTFILNDEYRFNNRTTISTSISATWGKRSTSALDWYNAPDPRPDY
                     YRYLPSYQLSSELQQQVLEAMQTNINKRQINWHGLYEVNRNSFAAIQDANGIQGNTVA
                     GKRSRYIQEERVLKNKSDNTLRAYRRDILQLLEFLDKLNLEIKGIKEKHIKDFLQDLK
                     RENISKRSINRKLSSIKGYFKFLERKDYIKEN"
     gene            complement(1557..2816)
                     /locus_tag="PLY81_02775"
     CDS             complement(1557..2816)
                     /locus_tag="PLY81_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF29183.1"
                     /translation="MNKLSKLAESLVGSEIVKLGNAISERIRNGETIYNYTIGDFNPS
                     VFPIPTLLEELIIEEYRNKSTNYPPADGVVELRNAISYYLHEWLHLNYNEKEIQVASG
                     GRPIIYTIFKAIVDEGDKVIYGVPSWNNNHYVHLTNGQHCLIDCTPENNFMPLPEDVA
                     KHIKGATLICLCTPQNPTGTTISEESLGKICDLIIEENNSRGEHEKKLYLMFDQMYWM
                     LTMDNIQHFNPVTLRPAMKQYTIFVGGISKSLCATGVRVGWGFGPEHVISKMKAILSH
                     IGAWAPMAEQKATAKYLVNTPALKEFVSNFNKEIDYRLQHIYAGFIALKNKGYQIDAI
                     TPQAAIYLTVKIDLVGKQTNEGNILYTQEDVTAYLLNEAKLAIVPFYCFGASKNSAWY
                     RLSIGTSHKQDIPIMLQKLENALSKLK"
     gene            3016..4539
                     /gene="lysS"
                     /locus_tag="PLY81_02780"
     CDS             3016..4539
                     /gene="lysS"
                     /locus_tag="PLY81_02780"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNF29184.1"
                     /translation="MSRQLSEQEIIRREKLQKITNAGIDAFPAPLYPVNTYSADIKNN
                     FTEENKDTFSKVCVAGRIMSINDKGKVFFIKIQDSKGIIQLYVKRDEICPEEDKSFWD
                     NIIKHGLDLGDIIGCTGYVFITKTGETSIHAQTITLLTKSLKPLPVVKRDEEGNVFDE
                     VTDPEFRYRQRYADLIINPSVKETFTKRTKLVNTIREYLNNLGALEVDTPVLQAIPGG
                     AAARPFITHHNALDVPFYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPEF
                     TILEWYTAYKDYFWMMEITEQLLEKIAVALNGTTTLQVGDKTISFKAPFRRISIFDAI
                     KENTGIDISEKDEIGLREVCKQLNIDVPPTIGKGKLIDEIFGATSEHTYIQPTFIIDY
                     PVEMSPLTKKHRSKKGLVERFELMINGKEIANAYSELNDPIDQRERFEEQAKLMERGD
                     DEAMFIDYDFLRALEYGMPPTSGIGIGIDRLVMLMTNQQSIQDVLFFPQMRPEKQEE"
     gene            4595..5632
                     /locus_tag="PLY81_02785"
     CDS             4595..5632
                     /locus_tag="PLY81_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNF29185.1"
                     /translation="MSDLSKFNPNSLGNPNNNIFGLPFTEEEASLILLPVPWEVTVSY
                     GAGTARSAEHIFNAALQVDLFDVDAPNVWKKGFYMLPVDKKLLMKSDYLRKEAELYID
                     FIAKEENFEGNKFMCKNLKEINEGCNYMNNFVYQQTKQLLGNNKFVGLIGGDHSTTFG
                     YIKSLSEKYNQFGILQIDAHCDLRVAYENFTYSHASIMYNILSEFEQVNKLVQLGIRD
                     YSDDEYNYIKNSNGRVTTFFDRDIKHRMYEGEIWKNIVSEIVDALPNNIYISFDVDGL
                     DPKLCPNTGTPVAGGFETEQIVYLFNKLKESNKKIIGFDLVETGVGENDWDSNVSARL
                     LWKMCNLVMNQ"
     gene            5643..6170
                     /locus_tag="PLY81_02790"
     CDS             5643..6170
                     /locus_tag="PLY81_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29186.1"
                     /translation="MQFNFSVFNNRKKQIWLLAYGLMIVNGLIFLLFALKSNIYKLAF
                     IYSALLITTTLVSIFIKKYKSIFYNVSYLLIAFSWIQLNYPWIGVLLIILLSVTKKFT
                     ADIAVTINEKEVEIKNVFSNNYQWSSFQNVILKDKLLTLDFKNNKILQVEILNKTTLE
                     QEKEFNEFCQSCLKT"
     gene            6179..7474
                     /locus_tag="PLY81_02795"
     CDS             6179..7474
                     /locus_tag="PLY81_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF29187.1"
                     /translation="MLTHTPYLLYSDGKGNIFEDTTLFALGRAGWDAFEVELNNWIEL
                     PDGGNLYELPGRKAIGIDTTTGEMRLCEKGWAVAAFIPPAHTSLFLAAYETDKNAPTL
                     PLFCYTAAGWYNNKTYVTAIRIEKDIRQDCAGYNNEKIKNGTDWLLQQYPNNRLVTHL
                     MNTCCMTYSCPAARNFSLNRWECPIPISPACNANCIGCVSFQPTDEDITSPQDRLTFK
                     PTVEEIVEYTVPHLMQAPFPIISFGQGCEGEPLLMWETIVPTIKEIRKHTNKGSININ
                     TNGSKPDAVKKLFESGLDSIRVSLNSARKDIYTKYYRPNNYCFEDIVESLKIAHQYNG
                     WSSINYFVFPGMTDSVEEYEALRKLIKDTNLKMIQWRNFNIDPDWYLGILNISETNEM
                     IGVKQMMHLIQEEFPDLKFGYFNPPIERIKGDFEVNFAH"
     gene            7501..8250
                     /locus_tag="PLY81_02800"
     CDS             7501..8250
                     /locus_tag="PLY81_02800"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNF29188.1"
                     /translation="MNLQELIIAAWNDRTLLKDETYTNAVKAVIEEVDKGKLRTASPI
                     AQGNWQVNEWVKQAILLYFGIQQMQTWDVAPFEFYDKMLLKKNYAALGVRAVPHAVAR
                     YGAYLAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKGVHLSGGVGIGGVLEP
                     LQASPVIIEDGCFIGSRCIVVEGVIVEKEAVLGANVVLTQSTKIIDVSGSEPIEMKGR
                     VPAQSVVIPGSYNKKFAAGEFGVGCALFTAA"
BASE COUNT         2904 a         1248 c         1366 g         2749 t
ORIGIN      
        1 gattttcttt aatataatct tttctttcca aaaatttgaa atatccctta atagatgaaa
       61 gttttctgtt aatggaacgt ttactgatat tctctctttt tagatcttgt aagaaatcct
      121 taatatgttt ttctttgatt cctttaattt ctaaattaag tttgtctaaa aattccaata
      181 attgcaaaat atcccttctg tacgcacgaa gagtgttatc actcttgttt tttagaactc
      241 tttcttcttg aatatatcta cttcttttgc ctgcaacagt attaccttga attccattag
      301 catcttgaat agctgcaaaa ctatttctat ttacctcgta caaaccatgc cagttaattt
      361 gccttttatt aatattggtt tgcatagctt ctaaaacctg ttgttgcaat tcgctgctta
      421 attgatagct tggtaaataa cgatagtaat ctggtctagg atctggtgca ttataccaat
      481 ctaaagcact agtacttctt ttaccccaag ttgcactaat agatgtagag attgttgttc
      541 tattattaaa acggtactcg tcgtttaaaa taaaggttgg ctggctgctt tttgcaatat
      601 tagcattgcg ttttttgcca ttttgtaaac cccaagttgg gttatagaaa ttatctcctg
      661 taattgttct taattcatcc actacaccac tttgtctacc actttcagtt ggtgcttcaa
      721 ataaagacaa agacagcaaa tgcttttgac caaaacgttt atctacgcca ataaaataac
      781 tatatccgtt atagtaagtt ccaggtacat atgcttcatc tgcacctcta aaactacctg
      841 caaaaacaaa tgcccatcct tttttgctta ttcctgtacc atgataaacg gttaaacgat
      901 gtgtataatt tctattgcta attgcatagc ctatttgtgt ttgttttctt tgcttacttg
      961 ctctagcatc tatagaagaa gaagatgcaa tatcgccaaa agcaaaagtg ctaggcctta
     1021 agcctaaaga tacatctctg ttacgcatta catcatttaa acctccccat aaaccaaatg
     1081 gtgtaaaacc attatctaaa ttatccattg gggttccatt caaataagta gtattcaaat
     1141 ctccatcata gcccctcatt ttaaatctaa aagcattaaa attaaaagac attaccgaga
     1201 aaaaaggatc tctgccagct gtaagtacac ttgaaacatt ttgtgcacca ccatcgcctt
     1261 gatcattttc atcaatggta actactgcaa tattatcaga cattgcatct ttaacatcat
     1321 cggtaattgt tgtatctaca acagcactac cattggtttt agcagatttg tttttttgtt
     1381 tttgtgccat taccgatatt ggcacagcca ttaatagtat aatggcaaat tttaaaaatt
     1441 tcaacataag tttaatttag tccaaaaggc aaatttaagt tcatttaagt aataattagc
     1501 ctcttttctt taaaaggtat ttctttctgt attcagtaag atttataaag aatttattat
     1561 tttaatttac ttaaagcgtt ttctaatttt tgtagcataa ttggtatatc ttgtttatgg
     1621 cttgtgccaa tacttaatct ataccaggca ctatttttag aagctccaaa acaataaaat
     1681 ggtactattg ctaatttggc ttcattaagt aaataagcag ttacatcttc ttgtgtatat
     1741 aaaatatttc cttcattagt ttgcttacca actaaatcta tttttacagt taaataaatt
     1801 gcagcctgtg gtgtaatagc atcaatttga tatcctttat tttttaaagc tataaagcca
     1861 gcataaatat gctgcagtct ataatctatt tctttattaa aattgcttac aaattccttt
     1921 aatgcaggcg tattaactaa atattttgca gtagcttttt gttctgccat gggtgcccaa
     1981 gcaccaatat gacttagaat tgctttcatt ttagaaatta catgctcagg cccaaaaccc
     2041 caaccaactc ttacgcctgt agcacataac gattttgaaa taccacctac aaaaatggta
     2101 tattgcttca ttgctggtct taatgtaacc ggattaaagt gttgaatatt atccatagta
     2161 agcatccaat acatttgatc aaacattaag taaagctttt tttcatgttc tcctctcgag
     2221 ttgttttctt ctataattaa atcgcaaatt ttacccaagc tttcctcaga aattgttgta
     2281 ccagttgggt tttgaggtgt acataaacaa attaaagtgg ctcctttaat atgcttggca
     2341 acatcttctg gcagtggcat aaaattattt tctggtgtac aatcaattaa acaatgttgc
     2401 ccattggtta aatgtacata gtggttatta ttccaacttg gtacgccata aattactttg
     2461 tctccttcat ctacaatagc tttaaaaata gtataaataa taggtcttcc tccactagca
     2521 acctgaattt ctttttcatt ataatttaaa tgtaaccatt cgtgtaaata atagctaata
     2581 gcatttctta actctacaac accatctgct ggtggataat tagtgctttt atttctatat
     2641 tcttctataa ttagttcttc taataatgtt ggaataggaa aaacggaagg attaaaatcg
     2701 ccaatagtgt aattataaat ggtttctcca tttctaatac gttcactaat tgcatttcct
     2761 aattttacaa tttcactacc taccagtgat tctgcaagtt tacttagttt attcataaag
     2821 ccatattttg gcaacgaaga taagtttttg aaaaaaatca aaaagtaaaa tactaatatc
     2881 tgctatttac taattattag attattggct ttcaagcaaa atattttaat tctaaacaaa
     2941 tgttgctaat taaataaaac ccttttttaa agccaatgct aaccttactt ttgcacttct
     3001 taaaaaagaa aaaatatgag taggcaactt agtgaacaag aaattataag aagagaaaaa
     3061 cttcaaaaaa ttaccaatgc aggtatagat gcatttcctg cccctttata tcctgtaaat
     3121 acctattctg cagatatcaa aaataatttt accgaagaaa ataaggatac ttttagtaag
     3181 gtatgtgtag ctggtagaat tatgagtatt aatgataaag gcaaagtttt ttttattaaa
     3241 atacaagaca gtaaaggcat cattcaacta tatgtaaaaa gagatgagat ttgccctgaa
     3301 gaagacaaaa gcttttggga taatataatt aaacatggct tagaccttgg cgatataatt
     3361 ggttgcaccg gttatgtttt tatcaccaaa acaggcgaaa catctattca tgcacaaact
     3421 attacattac taactaaaag cttaaaacct ttacctgttg taaaacgaga tgaggaaggt
     3481 aatgtatttg atgaagttac tgacccagaa tttagataca gacaacgtta cgcagactta
     3541 attataaacc catcggttaa agaaacattt acaaagcgta ctaaacttgt aaatactatc
     3601 agagaatatt taaataattt aggtgcatta gaagtagata ctcctgtttt acaagcaata
     3661 cctggtggtg cagcagctag accatttatt acacaccata atgctttaga tgtacctttt
     3721 tatttaagaa ttgccaatga attgtattta aaaagattaa ttgtgggtgg ctttgattgg
     3781 gtttatgagt ttagcagaaa ttttagaaac gaaggcatgg atagaacgca taatccagaa
     3841 tttaccattt tagaatggta cactgcgtat aaagattatt tctggatgat ggaaataaca
     3901 gaacaacttt tagaaaagat tgcagttgct ttaaatggta ctactacatt acaagttggc
     3961 gacaaaacaa ttagctttaa agcacctttt agaagaattt ctatttttga tgccataaaa
     4021 gaaaacacag gtattgatat ttctgaaaaa gatgaaattg ggttaagaga agtatgtaaa
     4081 caattaaata ttgatgtgcc gcctactatt ggtaaaggaa aactaataga tgaaattttt
     4141 ggtgctacta gcgaacatac ttacattcaa ccaaccttta ttattgatta ccccgtagag
     4201 atgagtccac ttaccaaaaa acatagaagt aaaaaaggat tagtggaaag atttgagtta
     4261 atgattaatg gcaaagaaat tgctaatgca tatagcgaat taaacgaccc aattgaccaa
     4321 cgtgaacgtt ttgaagaaca agctaaacta atggaacgtg gcgatgatga agcaatgttt
     4381 attgattatg attttttaag agcattagag tatggtatgc caccaacaag tggtattggt
     4441 attggtattg atagattagt aatgttaatg actaatcagc agagcattca agatgtttta
     4501 ttctttccgc aaatgaggcc agaaaaacaa gaagaataat tattatttaa aaagtaataa
     4561 aatagaatta acaaaagttg tataaaaaaa gattatgtca gatttatcaa aatttaatcc
     4621 aaactcttta ggtaatccta acaacaatat ttttggtttg ccatttaccg aagaggaagc
     4681 atcgcttata cttttacctg taccttggga ggttactgta agttatggag caggtactgc
     4741 aagatcggca gaacatattt ttaatgctgc attgcaagta gatttatttg atgttgatgc
     4801 acccaatgtt tggaagaaag ggttttatat gctacccgta gataaaaagc ttttaatgaa
     4861 aagcgactat ttgcgtaaag aagcagaatt gtatattgat tttatagcaa aagaagaaaa
     4921 ctttgaaggc aataaattta tgtgtaaaaa ccttaaagaa attaatgaag gttgtaatta
     4981 catgaacaat tttgtttatc aacaaaccaa acaactttta ggtaataata aatttgttgg
     5041 cttaattggc ggagaccaca gtaccacttt tggatacatc aaatcactct cagaaaaata
     5101 caaccaattt ggtattttgc aaatagatgc ccactgcgat ttaagagttg cctacgaaaa
     5161 ctttacgtac agccacgcaa gtatcatgta taatatatta tcagagtttg aacaagttaa
     5221 caagttagtt caattaggta ttagagatta tagtgatgat gaatacaatt acattaaaaa
     5281 cagtaatggc agagtaacca cattttttga tagagatatt aaacacagaa tgtacgaagg
     5341 agaaatttgg aaaaatattg tatctgaaat agtagatgcc ttgcctaata atatttatat
     5401 aagttttgat gtagatggtt tagaccccaa actttgccca aatacaggaa cgcctgtagc
     5461 aggaggtttt gaaacagaac agattgttta cttatttaat aaattaaaag aaagtaataa
     5521 aaagataatt ggttttgact tggtagaaac aggagtgggc gaaaatgatt gggatagcaa
     5581 tgtatctgca aggcttttat ggaaaatgtg caatttagta atgaaccaat aattagtaga
     5641 atatgcaatt taatttttct gtttttaata accgaaaaaa acaaatttgg ttattggctt
     5701 acggattaat gatagttaat ggattaattt ttttgctgtt tgccctaaag agtaatattt
     5761 ataaattagc ttttatctat tctgcattac taataactac tacacttgta agcatcttca
     5821 taaaaaaata caaatcaatt ttctacaacg tttcctattt acttatagct tttagttgga
     5881 tacaacttaa ttatccatgg attggagtat tgctaattat tttactatca gttacaaaaa
     5941 agtttacagc cgatattgcc gttacaatta atgagaaaga ggttgaaatc aaaaatgtat
     6001 tctctaataa ttatcaatgg agcagttttc aaaatgtaat acttaaagac aaattactta
     6061 ctttagattt caaaaataat aaaatacttc aggtcgaaat tcttaataaa actacactag
     6121 agcaagaaaa agaatttaac gaattttgcc aaagttgttt aaaaacttaa cacagtttat
     6181 gcttacccac acaccatacc ttttatactc tgatggtaaa ggaaatatat ttgaagatac
     6241 aacattgttt gctttaggaa gagcaggatg ggatgcattt gaagtggagt taaataattg
     6301 gatagaatta cctgatggtg gaaatttgta tgaactacca ggtagaaaag ctattggcat
     6361 tgataccaca acaggcgaaa tgcgtttgtg cgaaaagggt tgggctgttg cagcatttat
     6421 tccccctgca cacaccagtt tatttttagc agcttatgaa acagataaaa atgccccaac
     6481 tttgcctttg ttttgttata ctgctgctgg ttggtataac aataaaactt atgtaaccgc
     6541 aataagaata gaaaaagaca tacgccaaga ttgtgctgga tataacaacg aaaaaataaa
     6601 aaatggaacc gattggcttc ttcaacaata tccaaataac agattggtaa ctcatttaat
     6661 gaacacttgc tgtatgactt atagttgccc tgcagcaaga aacttttcct tgaacagatg
     6721 ggaatgtcca attccaattt caccagcctg taatgccaat tgtattggct gtgtatcctt
     6781 tcaaccaaca gatgaagata taacttcgcc acaagataga ttaactttta aacctactgt
     6841 agaagaaata gtagaatata cagtaccaca tttaatgcaa gcaccttttc ctataataag
     6901 ctttgggcaa ggctgcgaag gtgagccttt attaatgtgg gaaacgattg tacctactat
     6961 aaaagaaata agaaaacaca ccaataaagg aagcataaat attaatacca acggctctaa
     7021 accagatgct gtaaaaaaat tatttgaatc tgggttagat agtattagag tgagtttaaa
     7081 cagtgccaga aaagatattt acacaaaata ttatcgcccc aataattatt gttttgaaga
     7141 tattgttgaa agcctaaaaa ttgctcatca atacaatggt tggagtagta ttaattattt
     7201 tgtttttcct ggaatgacag atagtgtgga agaatatgaa gctcttagaa aattaataaa
     7261 agataccaat ttaaaaatga tacaatggag aaattttaat atagatccag attggtattt
     7321 aggcatatta aatatttcag aaactaatga aatgattgga gtaaaacaaa tgatgcattt
     7381 aattcaagaa gaatttcctg atttaaaatt tggatatttt aatccaccaa tagaaaggat
     7441 aaaaggggat tttgaagtga actttgcaca ctaaaaatta ttttaataaa aaaaacagaa
     7501 atgaatttac aagaactaat tatagcagcc tggaacgata gaactttatt aaaagatgaa
     7561 acttatacca atgcagtaaa agcagtaatt gaagaagttg ataaaggaaa attaagaaca
     7621 gcttcaccaa tagctcaagg taattggcaa gtaaatgaat gggtaaaaca agctatactt
     7681 ttatattttg gtattcaaca aatgcaaaca tgggacgttg ctccttttga gttttacgat
     7741 aaaatgctat tgaaaaaaaa ttatgcagca ttaggtgtaa gagcagtgcc acatgcagtt
     7801 gcaagatatg gtgcttactt agctaaaaat gttgtgctta tgccaagcta tgtaaatatt
     7861 ggggcttatg tagatgaagg cacaatggtt gatacttggg caactgtggg tagttgtgct
     7921 caaattggta aaggtgttca tttaagtggt ggtgttggta ttggtggtgt tttagagcct
     7981 ttacaagcaa gccctgttat tatagaagat ggttgtttta ttggtagccg ttgtattgtt
     8041 gtagaaggtg taattgtaga aaaagaagcc gtattaggtg ctaatgttgt tctcactcaa
     8101 tctacaaaaa ttattgatgt tagtggtagc gagccaatag aaatgaaagg acgtgtacct
     8161 gcacaaagtg ttgttattcc tggttcttac aataaaaaat ttgctgcagg agaatttggg
     8221 gtaggttgtg cattatttac agcggcttaa ggagcaggtg gtactgt
//
LOCUS       DAOQHP010000050         8159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4506_length_8159_cov_4.334403, whole genome shotgun sequence.
ACCESSION   DAOQHP010000050 DAOQHP010000000
VERSION     DAOQHP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4506_length_8159_cov_4.334403"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PLY81_02805"
     CDS             <1..1388
                     /locus_tag="PLY81_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNF29189.1"
                     /translation="NLKNYIPFVNEGTDVYDIIDWISKQSWCNGSVGMFGGSYTGFSQ
                     WAIAKKIHSALKTIVPQVAVMPGYDFPMENNVPLSHILSWSNDNIYKSKLLPRSLPFD
                     YYNSGVAFNKMDSLAGYKNPIFQNWLKHPAYDSYWQSMVPTSVEYAKINIPVLSTTGY
                     YDGSQIGAIQYFKLHNKYNKNANHYFVIGPYDHWGAQRKPAKNLMGYEIDSIANINMM
                     NLAYDWLDYVLKGKPKPALLKDRVNYQVMGTNEWKHSSTLDKINNDTLTFYLNKTRLT
                     LTKPQKKEFQKQTVDFNDRENQNNYYTPEIIFDTLDASNGLVFTTDIFQKEYIVNGSF
                     SGNLWVSINKKDMDVSMALYEKMENGKYFFLTRYVGRASFAKDNSKRQLLQPNKKENI
                     PFNNTRFVSKKINKGSRLVILLNINKHPFEIINYGSGKPVSEETIKDAGKPLQIKWHN
                     DSFIKIPIWKE"
     gene            complement(1463..1924)
                     /locus_tag="PLY81_02810"
     CDS             complement(1463..1924)
                     /locus_tag="PLY81_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF29190.1"
                     /translation="MAVKTFLLLMACFLAGALTPIQAGFNFRLAKAVNNTISAALISF
                     LVGTVILLAYLAFTKQLKLDLVTVAKTEPFWVWLGGVIGAFFVVTLTFVVPKVGASLS
                     FSLIIAGQLIISIIIDHYGLLGVPVTPINMKRIAGILLLALAIWLIKEKNV"
     gene            complement(2033..3262)
                     /locus_tag="PLY81_02815"
     CDS             complement(2033..3262)
                     /locus_tag="PLY81_02815"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNF29191.1"
                     /translation="MAVKIKVANPIVELDGDEMTRIIWKFIKDKLILPYIEVDIKYYD
                     LGVEYRDATNDQVTIDAANAIKQYGVGIKCATITPDEQRVEEFKLKQMWKSPNGTIRN
                     ILDGTVFREPIVCNNVPRLVPNWTAPICIGRHAFGDQYRATDFVTKGKGKLTIKFEGE
                     DGNVIEHEVFNFKSDGVALAMYNTDESIIGFARSCFNTALQKKWPLYLSTKNTILKKY
                     DGRFKDIFEEIYQQEFKSKFEAAGITYEHRLIDDMVASALKWNGNFVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSTLVTPDGKIMEAEAAHGTVTRHYREHQKGRPTSTNPIASIFA
                     WTRGLEFRGKLDNNNDLIKFAHALEQVCIETVESGKMTKDLAVCIHGNNVKHGEHYLY
                     TEEFLDALDNALQAKLA"
     gene            complement(3415..4977)
                     /locus_tag="PLY81_02820"
     CDS             complement(3415..4977)
                     /locus_tag="PLY81_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF29192.1"
                     /translation="MRILTGLKNSALLVATLGILASCNSFKKKQQKSDVTGWAYNDKK
                     NGGFTVAKPKNIKTAPGLVFVQGGTFTMGATEEDVMGDWNNIPKRITVNSFFIDKTEV
                     ANVHYREYIFWMQNTFGEDDKYIQLMEDIKPDTLVWRSELSYNEPYVEYYFSHPSYNY
                     YPVVGVTWKQARDFCIWRTDRVNEKALIDKKIINAKDYAKGGMNNVGEDNFNTKAYLL
                     GLYNPKTPPSKGSPLKDSKGQPRQAKFDDGILYGDYRLPTEAEWEYAAYGYIMENPQK
                     RRNEKTRGEEVIGNKQVYAWRNAGYDNLRSTKRGAFQGAFLANFKRGSGDNMGVAGGL
                     NDRSAIPAEINSFYPNGFGIYNMSGNVSEWVADVYRPLTSLEGEDFNYYRGNVFQKVK
                     KDSSGEMQKDSLGRVVMVNEPDSVLRKRRNYQKSYAIDYLDGDQIADGRSAVYNYGTT
                     TLVSDKSRVVKGGSWNDRPYWLSPGARRFLEETQSSSTIGFRCAMTHYGGPESTKFGK
                     TGNNFPARRQKK"
     gene            complement(5329..7488)
                     /locus_tag="PLY81_02825"
     CDS             complement(5329..7488)
                     /locus_tag="PLY81_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNF29193.1"
                     /translation="MKKIIVSLVFALTAFRSYADEGMWLPMLLGQQVYKDMVKKGLKL
                     KPEQLYSINKSSIKDAIIIFGGGCTGEIVSNQGLIFTNHHCGYGAIATASTVEANYLR
                     DGFWAADKSKEISTNLSVKFLNRIEDVTEKINAELKNLSGAERDKKYREVVAKIVKEI
                     VGTSEFKDAVVASMFKGNQFFVFVYDVYKDIRLVGTPQESIGKFGGDTDNWEWPRHTG
                     DFSVFRVYTNADGSPANYNANNIPLKPKYHLPVSIKGIKDGDYSMIFGYPGGTNRYET
                     SYGVKLATDINNPSLVALRAERLKYMYNQMKNDAAVKLQLASRYASIANYWKFFDGET
                     RQLLKFDTYGAKQKDEAAFIEWAKDKAEYKNIFSDYEANYNAWTPFAKGRQYLNEGIF
                     GSPLVAYVSSWASLERRLARKDYNPEEIKKAMDAADKARKKFLEDENKKSDQQILATV
                     VSMFYSNIDKSQHPKGFYEDLFNNYNDIEQNPYKMFAADVFENTMAFDDAKWKAFMEK
                     PSAINIVEDPAYKVAKAFISNYSDNYAPKYTDFVNKNNDLGRLYMKGLFEMNPTKMNG
                     TYPDANFTMRVSYGNVKSYKPKDAVTYDYYCTLAGTVAKYKKGDYEFDLPAKLLELYK
                     NKDFGDYADKKLGDIVVNFITTNDITGGNSGSPVIDAKGNLIGLAFDGNSEALSHKLA
                     FDKELNRTICVDVRYVLWCIEKVGGAKHIIDELTLVK"
     gene            complement(7606..>8159)
                     /locus_tag="PLY81_02830"
     CDS             complement(7606..>8159)
                     /locus_tag="PLY81_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNF29194.1"
                     /translation="VQENNLETLFNHVEFTNNWKGKTEQLVLQIFYHTGIRLSELIQL
                     KTVNIDFYYQHIKVLGKGNKERIIPISKELLSDIKEYISLKPEAIKQVPQLLVTEKGT
                     ALQPRSVYAMVKKYLSMVTTVQKRSPHVLRHSFATHLMNSGADLNAVKELLGHSSLAA
                     TQVYTHNTIEQLKDIFKKAHPKA"
BASE COUNT         2652 a         1421 c         1124 g         2962 t
ORIGIN      
        1 ctaacttgaa aaattacata ccatttgtaa atgaaggaac agatgtttac gacattattg
       61 attggataag taaacaaagt tggtgtaatg gaagtgttgg aatgtttggt ggaagctaca
      121 ctggattttc tcagtgggct attgctaaaa aaattcattc agctttgaaa actattgtac
      181 cacaagttgc tgtaatgcct ggttatgatt ttccaatgga aaataatgtt ccattatctc
      241 atatattaag ttggagtaat gacaatattt ataaaagcaa acttcttccc agaagtttac
      301 cttttgacta ttacaatagt ggtgtagcat tcaataaaat ggacagttta gcaggttata
      361 aaaatccaat tttccaaaat tggttaaagc accctgctta tgatagttat tggcaatcaa
      421 tggtaccaac atcagtcgaa tatgctaaaa tcaatattcc tgttttatca acaacagggt
      481 attatgatgg ttctcaaata ggagctattc aatatttcaa actacataat aaatacaata
      541 aaaatgcaaa tcattatttt gtaattggcc cttatgatca ttggggtgca caacgcaaac
      601 ctgctaaaaa cttaatgggt tatgaaattg atagtattgc caatattaat atgatgaatt
      661 tggcttacga ttggcttgat tatgtactta aaggcaaacc aaagcctgca ctattgaaag
      721 acagagtaaa ctatcaagta atgggaacaa acgaatggaa acatagttca acacttgata
      781 aaattaacaa cgacacttta actttctatc taaataaaac aagactaaca ttaaccaagc
      841 cccaaaaaaa agaatttcaa aaacaaactg ttgactttaa cgacagagag aatcaaaata
      901 attattatac tcctgaaatt atttttgaca cacttgatgc aagcaatgga ttagttttta
      961 caactgatat ttttcaaaaa gaatatattg ttaatggctc tttttcagga aatctttggg
     1021 taagtataaa taaaaaagat atggacgttt caatggcact ttatgaaaaa atggaaaatg
     1081 gtaaatattt ttttctaaca agatatgttg gtagagcaag ttttgcaaaa gacaattcta
     1141 aacgacaact actacaacca aacaaaaaag aaaatattcc ttttaacaac acccgttttg
     1201 taagtaagaa aattaacaaa ggtagcaggc ttgttatatt gctaaacatt aacaaacatc
     1261 cttttgaaat aataaattat ggttcaggta aacctgtatc tgaagaaaca ataaaagatg
     1321 ctggaaaacc attacaaata aaatggcaca atgatagttt tattaaaatt ccaatttgga
     1381 aagaatagta agatagaaac aaactagcta ttaattatac aataaaagat acattgtgat
     1441 tgatttttct ttcacaaaaa agttaaacat tcttttcttt tattagccag attgcaagtg
     1501 ctaataataa aataccagca attcttttca tatttattgg ggttacaggc acacctaata
     1561 atccataatg atcaataatt attgaaataa ttaattgccc tgcaatgatt aaactaaaag
     1621 ataatgatgc cccaactttt ggaactacaa aggttaatgt aacaacaaaa aaagctccaa
     1681 ttacaccgcc taaccaaacc caaaaaggtt cagttttggc aactgtaact aaatcaagtt
     1741 ttaattgttt ggtgaaagct aaataagcca ataaaattac agtgccaact aaaaaagaaa
     1801 ttaaagctgc acttatagta ttattaactg cctttgctaa cctaaagtta aacccagcct
     1861 gtattggtgt taatgctcct gctaaaaagc aagccattaa aagcaaaaaa gtttttactg
     1921 ccataatttt atataagttg ctaaaattat aattttaaaa attagtttta aaaaaaacaa
     1981 taaaaaaact gccgtaaatt tatcggcagt tttataaaat agttatctac tattaagcta
     2041 atttggcttg taatgcgtta tctaaagcat ctaaaaattc ttcggtatat aaataatgtt
     2101 ctccatgttt tacattatta ccatgaatac aaactgctaa atcttttgtc attttaccac
     2161 tttcaacagt ttctatacaa acttgttcca aagcatgagc aaattttatt aaatcattgt
     2221 tgttgtctaa tttacctcta aactctaaac ccctcgtcca tgcaaaaata gatgcaatag
     2281 ggttggtaga agttggtctg cctttttgat gctcacggta atgccttgta actgttccat
     2341 gtgcagcttc tgcttccata atctttccat caggggtaac taatgtagat gtcattaaac
     2401 ctaaactacc aaaaccttga gctactgtat cgctttgtac atctccatca taatttttac
     2461 aagcccatac aaaattgcca ttccatttta aagcactagc aaccatatca tcaattaaac
     2521 gatgctcgta agtaatgcct gctgcttcaa attttgattt aaattcttgc tgataaattt
     2581 cttcaaaaat atctttaaac ctgccatcat attttttaag aatagtattt ttagtactta
     2641 aatataatgg ccattttttt tgtaaagcag tattaaagca acttctggca aagccaataa
     2701 tgctttcatc tgtattatac atggctaatg caacaccatc gcttttaaag ttaaatactt
     2761 catgttctat tacattcccg tcttctcctt caaacttaat ggttaattta ccttttcctt
     2821 tggttacaaa atctgttgct ctatattggt ctccaaaagc atgtcggcca atacaaattg
     2881 gtgctgtcca gtttggaact aaacgaggta cattgttgca aacaattggc tcacgaaaaa
     2941 ccgtaccatc taaaatattt ctaatagtac cattggggct cttccacatt tgttttaact
     3001 taaattcttc aaccctttgt tcatctggtg taatagttgc acatttaata ccaacaccat
     3061 actgttttat tgcattggct gcatcaattg ttacttggtc gttagttgca tctctatatt
     3121 caactcctaa atcgtaatat ttaatgtcaa cttcaatgta tggaagtatt aatttgtctt
     3181 taataaattt ccaaattatt cgtgtcatct cgtcgccatc taattctact attggattag
     3241 caactttaat tttaactgcc ataatattat aatttatttt taaaggtttg caaatgtagt
     3301 atataaatag attgcactta gaatcttaaa taataatata aaaaaagcca ctaaaaaaat
     3361 cagtgacttt attttattcc tgtttaaaac aagaaagctg tatttacctt tcccttattt
     3421 cttttgtctt cttgcaggaa aattgtttcc tgtttttcca aacttagtgc tttctggacc
     3481 tccataatgt gtcattgcac aacggaaacc aatggtactg ctgctttgtg tttcttctaa
     3541 aaatcttctt gcacctgggc ttagccaata tggtctatcg ttccaactac caccctttac
     3601 tactctagat ttatcagaaa ctaatgttgt tgtaccatag ttataaactg cacttctacc
     3661 atcagctatt tggtctccat ctaaataatc tatagcataa gatttttggt agtttctgcg
     3721 ttttcttaat acgctatctg gctcgttaac cataactact cttcctaatg aatctttttg
     3781 catttcgcca gatgaatctt ttttaacttt ttggaaaacg ttacctctat aatagttaaa
     3841 atcttctcct tctaaacttg ttaatggtct gtaaacatcg gcaacccact cacttacgtt
     3901 accactcata ttataaattc caaaaccatt aggataaaaa gaattaattt ctgcaggtat
     3961 tgcagaacgg tcatttaaac cacctgcaac tcccatatta tcgccagaac ctcttttaaa
     4021 gtttgctaag aatgcccctt gaaaagcacc tctttttgta gaacgtaagt tgtcgtaacc
     4081 tgcatttctc caagcataaa cttgtttgtt acctataact tcttcgcctc tggttttttc
     4141 atttctacgt ttttgagggt tttccataat gtagccataa gctgcatatt cccattcagc
     4201 ttcagttggt aaacgataat ctccatataa aataccatca tcaaatttag cctgacgagg
     4261 ttgtccttta gaatctttta aaggagagcc tttgcttgga ggtgttttag gattatataa
     4321 gcctaataaa tatgcttttg tattaaagtt atcttcgcca acattattca tgccgccttt
     4381 ggcataatct tttgcattaa tgattttttt atcaattaaa gctttttcgt taactctatc
     4441 ggtacgccaa atacaaaaat cacgagcttg tttccaagta acacctacta ctggataata
     4501 gttataactt gggtggctaa aataatactc aacgtatggt tcattataag ataattcact
     4561 tctccaaaca agggtatctg gtttaatatc ttccattaat tgaatatatt tatcatcttc
     4621 cccaaaggta ttttgcatcc aaaaaatata ctcacgatag tgtacattcg ctacttctgt
     4681 tttatcaata aagaacgagt ttactgtaat acgttttggg atattattcc aatctcccat
     4741 tacatcttct tcagtagcac ccattgtaaa tgtaccacct tgaacaaaca ccagtccagg
     4801 tgctgtttta atattttttg gctttgctac cgtaaagcct ccattctttt tatcattgta
     4861 agcccagccg gttacatctg acttttgttg tttcttttta aaagaattac aagatgctaa
     4921 gatgccaagt gttgcaacta gcaatgcaga atttttaagc cctgttaaga ttcgcatttt
     4981 tgaaagtttg agtgtactaa aacgttgatt ttaaaaaaat attgtctatt taagagttgc
     5041 gaatataaaa gtaattattg aattatttct aaactacaac taaaaataat ttaacaaatt
     5101 tatttagcat tgatttacaa gcaaaaattt taaaaaaata ctactttccc tgtaaatatt
     5161 tttggcgaaa tggtataaat ttaatttcga aaaaagtatt gattgtcaat cacttatttt
     5221 ttagactcta taatatttga ggttaagttt ttgaaaaaat gattacatat atataaaaaa
     5281 accctgaaat gtttcagggt tttagcaaca attactttaa aatgtatttt atttaactaa
     5341 agtaagttca tcaattatgt gttttgctcc acctactttt tctatacacc ataatacata
     5401 acgaacatca acacatattg tacggtttag ttctttatca aatgctaatt tgtggcttaa
     5461 ggcttcggaa ttgccatcaa atgccaatcc aattaaattg ccttttgcgt ctattactgg
     5521 gcttccagag ttaccacctg taatatcatt agtagtaata aagttaacca caatatcgcc
     5581 taatttttta tcggcatagt ctccaaaatc tttgttttta taaagctcaa gtaatttagc
     5641 aggcaaatca aattcataat cgcctttttt atatttagct actgtacctg ctaaagtgca
     5701 atagtaatcg taagttactg catcttttgg tttatagctt tttacattac cataagaaac
     5761 acgcatggta aaatttgcat ctggataagt tccattcatt ttagttgggt tcatttcaaa
     5821 taaacctttc atgtacaatc ttcctaaatc attgttcttg tttacaaaat ctgtgtattt
     5881 gggtgcataa ttatctgaat agttgctaat aaaggctttt gctactttgt aagcagggtc
     5941 ttctacaata ttaattgcac ttggtttttc cataaatgct ttccattttg catcatcaaa
     6001 agccattgtg ttttcaaaca catctgctgc aaacatttta taagggtttt gctcaatatc
     6061 gttgtaatta ttaaacaaat cttcgtaaaa accttttgga tgttggcttt tatctatatt
     6121 gctataaaac atagaaacta cggttgctaa tatttgctga tcgctttttt tgttttcatc
     6181 ttctaagaat ttttttcttg ctttatcagc agcatccatt gcttttttaa tttcttccgg
     6241 attataatct tttcttgcta atcttctttc taagcttgcc caacttgata cataagcaac
     6301 taatgggctt ccaaaaattc cttcgtttaa atattgtctt cctttagcaa aaggtgtcca
     6361 tgcattatag ttagcttcat aatcgctaaa aatattttta tattctgctt tatcttttgc
     6421 ccattctata aatgcagctt catctttttg ctttgcacca taagtatcaa attttaagag
     6481 ttgtcttgtt tctccatcaa aaaatttcca gtagttggca atacttgcat aacggctggc
     6541 taattgtaat tttacggcag catcattttt catttggtta tacatgtatt ttaatctttc
     6601 tgctcttaat gctactaaag atggattatt gatatctgtt gcaagcttaa caccataaga
     6661 tgtttcatat ctgtttgtac cgcctgggta gccaaaaatc atgctataat caccatcctt
     6721 aattccttta atgcttacag gtaaatgata ctttggcttt aatggtatgt tgttagcatt
     6781 atagtttgct ggtgaaccat ctgcgtttgt ataaacacga aaaacagaaa aatcgcctgt
     6841 atggcgaggc cactcccaat tatctgtatc gcctccaaat tttccaatac tttcttgtgg
     6901 tgtacctact aggcgaatgt ctttatacac atcatacaca aaaacaaaaa actgatttcc
     6961 cttgaacata ctagctacaa ctgcatcttt aaattcgctt gtgccaacta tttcttttac
     7021 aattttagca actacttctc tatacttttt atctctttcg gctcctgata aatttttaag
     7081 ttctgcatta attttttctg ttacatcttc aattctgttt aagaacttaa cacttaaatt
     7141 agtggatatt tctttgcttt tatctgctgc ccaaaatcca tctcttaagt agtttgcctc
     7201 tactgtactt gcagtagcaa ttgcaccata accacaatgg tggttggtaa aaattaagcc
     7261 ttggttgctt actatttcgc ctgtacaacc tccaccaaaa ataataatag catcttttat
     7321 agatgattta ttaatactat aaagctgttc tggttttagc tttaagcctt ttttaaccat
     7381 atccttataa acttgttgcc ccaataacat tggtagccac atgccttcat ctgcataact
     7441 acgaaatgca gttaaagcaa acactagaga aacaattatt tttttcatat tgcaaatttt
     7501 attgcggcaa agtaataaat aatgtgtttt taaaaatttg tttttaaaaa gaaattttta
     7561 attaatatat aaatggtaag taaattggat gaattagtat ataatttaag cctttggatg
     7621 ggctttttta aatatatctt tcaattgttc tattgtatta tgtgtataaa cctgtgttgc
     7681 tgcaaggctg ctatggccaa gtagctcttt tactgcattt aaatcggcac cactattcat
     7741 taaatgggtt gcaaaactat gccttaatac gtgagggctt cttttttgaa cagtggtaac
     7801 catgcttaaa tattttttta ccatggcata tacactgcgt ggttgtaatg ctgtaccttt
     7861 ttctgttact aataattgtg gaacttgctt tatagcttct ggttttaaac ttatatattc
     7921 ttttatatca cttaaaagtt ctttagatat aggaataatt ctctctttat tgcctttgcc
     7981 taatacttta atatgctgat aataaaaatc aatatttaca gttttaagtt gtattaattc
     8041 acttagccta atacctgtat gataaaatat ttgaagtact aattgttctg ttttaccttt
     8101 ccagttattg gtaaactcta catgattaaa taaggtttct aaattatttt cttgaacaa
//
LOCUS       DAOQHP010000051         8140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4526_length_8140_cov_4.060730, whole genome shotgun sequence.
ACCESSION   DAOQHP010000051 DAOQHP010000000
VERSION     DAOQHP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_8140_cov_4.060730"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLY81_02835"
     CDS             complement(<1..221)
                     /locus_tag="PLY81_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF29195.1"
                     /translation="MKHILTIFFSIIILNAFSQETVYPAKIQTETIALTNATIHIGNG
                     DIIENGMIVFSKGKIIDIRPTAPIADIKV"
     gene            complement(274..3339)
                     /locus_tag="PLY81_02840"
     CDS             complement(274..3339)
                     /locus_tag="PLY81_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF29196.1"
                     /translation="MFTKLKLLIVACFCIAIINAQETFPVNGTKDVRINCYAFTNATI
                     IQNATTTLQNATLVIKQGKIVGINIPVPKEAVVVDCKGKFIYPSFIDLFSEYGLKPVA
                     NTGFRGFGQPRQFESKTKGAYNWNQAIKPEINAFEKFEGNEKLAKELRAIGFGTVVTH
                     EKDGIARGTGALVTLGDDKENFELVKEKVGAFYSFNKGSSTQDYPGSLMGVIALIRQT
                     YIDAAWYKNKPTTEGTNISLQAWNNNLSLPQIFDIGNDKYNALRANKIAKEFGATYIL
                     NGSNDMYQRLDEIKAMNSSIILPLNFPAPMDVEDPNDARMVAYADLKHWEMAPYQPAM
                     FEKENINFSLTTNGLTATNDFLINLRKAIHNGLSEKTALDALTRLPATWINAYDITGS
                     LEPGKLANFFITNTNIFSDSATIFQNWVQGKKYIVKENGWNDYNGTYVFTVTTKGKTN
                     FYEATIKGNSEKPTLTLKPYEDSVNIPTTLTLNNQLVKINWSAKADKGKQNNLSGIIG
                     SDAWAGNGYLANGDVVIWKLFAKKENEGKSAKVDTSNKNTTHSTDKTAITINDVVYPF
                     SGYGYKKEFAPMQEKILIRNVTVWTNEKDGKLENTDVIINKGKIERIGKHLPVTLDMS
                     WKTIDGTGKHLTAGIIDEHSHIAITGGVNECTNAVTSEVRIGDILDPEDINIYRQLSG
                     GVTTSHLLHGSCNPIGGQTQLIKLRWGKGAEELKFQGSDGFIKFALGENVKSSRSTNN
                     TRFPDTRMGVEQVYADAFQRAIDYKQLPVGKRRDLQLDALVEILNNTRFITCHSYVQS
                     EINMLIKVAEKYNFKINTFTHILEGYKVADKMKKNGINASTFSDWWAYKLEVQDAIAY
                     NAAIMQKMGLNVAINSDDAEMARRLNQESAKSIKYGGVSEEEALKMATLNPAKMLHID
                     NKVGSIKVGKDADVVLWNDHPLSIYAKTLYTIVDGVIYFDREKDEELRKNIAQEKSRL
                     IQKLLAEKKKPFNAGKMQPARPRMEVINECEEDHKHNHSIFEREDEFSY"
     gene            complement(3435..4310)
                     /locus_tag="PLY81_02845"
     CDS             complement(3435..4310)
                     /locus_tag="PLY81_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNF29197.1"
                     /translation="MQLHPVDIVQNISAEEFKKKYYLTSKPLVIKSLSQQWSAYSKWN
                     WDYFKTLVGSQKVALYNNIKSNAYTPINTADDYKTFGEYIDMIKAGPAGWRIFLFNIF
                     DHAPQLINDFTWPEHLTKGFVKKYPMLFTGGATSITHMHFDIDLSHIFHTQFIGRKRV
                     LLFPFKEQYKLYRKPFEVLSFVDFSNYYDEANSKIDYTKFPILKNAQGMEVILEHGDT
                     LFMPAGYWHHMEYLDSGFAMSLRALQPSITGKLAGVWNLFGMRNIDTLLKKTIPQPWY
                     NWKQKKAYARAAKEI"
     gene            complement(4373..5191)
                     /locus_tag="PLY81_02850"
     CDS             complement(4373..5191)
                     /locus_tag="PLY81_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF29198.1"
                     /translation="MLFNNKTVFISGASRGIGKAIALKLASYGANIVIAAKSTTENSK
                     LGGTIYTAAKEIEAAGGKALAVQTDVRNEEQIANAVKQTIETFGGVDIVINNASAIQL
                     TNTAQTEAKRFDLMHSVNVRGSFLVVQHFLPYLQKGNNPHILTLSPPVNLISKWLSPH
                     IAYTMSKFNMSMMALAWAEEFKNFGIASNALWPQTTIDTAAVRNLLGGETLANMSRTP
                     QIIADAAFYILSKQNLEYTGNTFIDEIVLAKEGITNFDKYAVVPGAKLYTDLFL"
     gene            complement(5206..6054)
                     /locus_tag="PLY81_02855"
     CDS             complement(5206..6054)
                     /locus_tag="PLY81_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNF29199.1"
                     /translation="MNTTSYQIKLDHFEGPFDLLLFFIERDELDIYNIPITKITNDFL
                     NYIHSQEKLNIELSSEFILFVSTLMRIKAKLLIPRKELDADGKEIDPRQELVDKILEY
                     KKFKEASAKMADMEALRMLMVKRGNLQKELNEIGEQAGEGTEIQNITLFKLMKSFEKV
                     MQRLNERQNKPVHTVVRYNYTMEGSRDYMLHFIQKEKTVSFEKVFDVCESRVHAIFLF
                     LSMLELTQQRFMKLLIGEGRNNFIIEWNENREQEIQEEGLDINELNNMPTSFPDSIQD
                     DIATLN"
     gene            complement(6149..7060)
                     /locus_tag="PLY81_02860"
     CDS             complement(6149..7060)
                     /locus_tag="PLY81_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNF29200.1"
                     /translation="MQTTQHLLMIKPVHFTYNAQTAVNNAFQNAAADSNTQQNALNEF
                     NNFVELLMQNGIDVTVIEDTLQPHTPDSIFPNNWISFHANGTILLYPMYAENRRQERK
                     PHVLENISKKFSISTTIDLTNYEKSNLFLEGTGSMVLDREKNIAYACISPRTDATILE
                     EFCNKMHFTPMLFKANDSNNSPIYHTNVMMCVADKYVVICLDAITDHQERSTVIHSIE
                     SSGKQIVPITLQQMNKFAGNMLQVNNQKGEKFLIMSSQAYQALTPQQINQLQSYNHIL
                     HSNLTTIETNGGGSARCMMAEVFLPLK"
     gene            complement(7081..>8140)
                     /locus_tag="PLY81_02865"
     CDS             complement(7081..>8140)
                     /locus_tag="PLY81_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNF29201.1"
                     /translation="ITGIFPAEENGTKEDQYIFPPIPNFIFTRDNAIMMKDHILLSRM
                     ATTARKRESLISKFLALYFLFRDNPEKVIEVIEESDYFLYEEEERKQRIITIEGGDIM
                     MIHPTHLLVGCSERTSASAINEIIHTIFNNSHLEIEKISVVKIPKKRAQMHIDTIFTQ
                     VKRNVWVLYGRYSDTILKAEEDTKKSYLKKLSHVPKKTELEEVEIFQFYKNKKEPYAK
                     NKDYSISNAPAGLESLLRHISEVDFGVKPEDVKIIYSGNNEFPHDEREQWTDSSNVVA
                     LKEGVVISYDRNDKTTETFKNAGFNIVTTTQVFEMFEKGIDPHSLEDTVILIPSAELS
                     RARGGSHCMSMPLLRDKW"
BASE COUNT         2414 a         1378 c         1163 g         3185 t
ORIGIN      
        1 attactttaa tatctgcaat tggagctgtt ggtctaatgt caattatttt tcctttagaa
       61 aaaacaatca tgccattttc tataatatct ccattgccaa tatgtatagt tgcattagtt
      121 aatgcaatag tttcggtttg gatttttgca ggatatactg tttcttgaga aaaagcattt
      181 aatataataa tgctaaaaaa tatggttagt atatgcttca tataataatt ttaaaatgtt
      241 aaacaattca attttttatt tatgaattaa ggattaatag ctaaactcat cttctctttc
      301 aaaaatgctg tgattatgtt tatggtcttc ttcgcactca tttataactt ccattcttgg
      361 gcgtgcaggt tgcattttac ctgcattaaa tggtttcttt ttttctgcta ataatttttg
      421 aatcagtctt gatttttcct gagcaatatt ttttcttaac tcttcatctt tttctctatc
      481 aaaatatata accccatcta caatggtgta caatgttttt gcataaatgc ttagaggatg
      541 atcattccat aacacaacat cggcatcttt acccactttt atgctaccta ctttattatc
      601 tatatgcaac atttttgcag gattaagcgt tgccattttt aaagcttctt cttcgctaac
      661 gccaccatat ttaatagatt ttgcagattc ttgatttagc ctacgagcca tttctgcatc
      721 atcgctattt atagctacat ttagacccat tttttgcata atagcagcat tgtatgcaat
      781 tgcatcttgt acttctaatt tatatgccca ccaatcgcta aatgtggaag cattgattcc
      841 attttttttc attttatctg ctaccttata tccttctaaa atatgtgtga aagtgtttat
      901 tttaaaattg tacttttcgg caactttaat aagcatattt atttcgcttt gtacatagga
      961 atggcaagta ataaatcttg tattatttaa tatttctacc aatgcatcta attgcaaatc
     1021 tcttcttttg ccaactggta gttgtttgta atcaatggct ctttgaaaag catctgcata
     1081 cacttgttca acacccattc tggtatcagg aaaacgagtg ttattagtac ttctggagct
     1141 ttttacattt tctcctaatg caaattttat gaacccatcg gatccttgaa attttaattc
     1201 ttctgcacct ttaccccaac gtaattttat taattgtgtt tgcccaccta ttggattaca
     1261 gcttccatgt aataaatgag atgttgtaac accgccactt agttgtctat aaatatttat
     1321 atcttctggg tctaaaatgt cgcctattct tacttcggat gttactgcat tggtacattc
     1381 attaacaccg cctgttattg ctatatgaga atgttcgtct ataattcctg cagttaaatg
     1441 tttaccagtt ccatcaatgg ttttccaact catatctaat gttactggca agtgttttcc
     1501 tattctttca atttttcctt tattaataat tacatctgtg ttttctaatt ttccatcttt
     1561 ttcatttgtc cacactgtaa catttcttat taatattttt tcttgcattg gtgcaaattc
     1621 ttttttatat ccatagccac taaaaggata tacaacatcg ttaattgtta tggcagtttt
     1681 atctgttgaa tgtgtagtgt ttttattgga tgtatctact tttgccgatt tcccttcgtt
     1741 ttctttttta gcaaataatt tccaaattac cacatcgcca tttgctaaat agccattgcc
     1801 tgcccaagca tcagagccaa ttattccgct taaattattt tgttttcctt tatcggcttt
     1861 tgcagaccaa ttaattttta ctaactgatt attgagtgta agggttgtag gaatatttac
     1921 tgaatcttca taaggtttta atgttaatgt tggtttttca gaattgcctt ttatggtagc
     1981 ttcataaaaa tttgttttgc cttttgtggt aactgtaaat acataagttc cgttataatc
     2041 attccaacca ttttctttta caatatattt ttttccttga acccagtttt gaaaaatagt
     2101 tgcagaatcg gaaaaaatat ttgtgttggt aataaaaaag ttagccaatt ttccaggttc
     2161 taaacttcct gttatgtcat aagcatttat ccatgttgct ggtaaacgtg ttaaagcatc
     2221 taatgctgtt ttttcagata agccattatg aattgctttt cttaagttaa ttaaaaaatc
     2281 gtttgttgct gttaagccat ttgtggttag agagaaatta atattttctt tttcaaacat
     2341 tgctggttga taaggtgcca tttcccaatg ttttaaatcg gcataagcaa ccattcttgc
     2401 atcattaggg tcttctacat ccattggtgc aggaaaattt agtggtaata ttatagatga
     2461 attcattgct ttaatttcat ctaacctttg gtacatatca ttgctgccat ttaaaatata
     2521 agttgctcca aactcttttg caattttatt tgctcttaat gcattgtatt tatcatttcc
     2581 aatatcaaaa atttgaggta gggataaatt gttgttccat gcttgtaagc taatatttgt
     2641 gccttcggtg gttggtttgt ttttatacca tgctgcatca atatatgttt ggcgaataag
     2701 tgcaataaca cccattaaag agcctggata atcttgtgta gaacttcctt tattaaaaga
     2761 ataaaaggct cctacttttt ccttaactaa ttcaaaattt tctttatcat cgccaagtgt
     2821 aactaatgct ccagtaccac gtgcaatgcc atctttttcg tgtgttacaa ctgtgccaaa
     2881 accaattgct cttaactctt tagctaattt ttcgtttcct tcaaattttt caaaagcatt
     2941 tatttctggt tttattgcct gattccaatt ataagctcct tttgttttag attcaaactg
     3001 ccgaggttgc ccaaaacctc taaaccctgt atttgcaaca ggttttaggc catattccga
     3061 aaataaatct ataaaagacg gatatataaa ctttccttta caatctacca caactgcctc
     3121 tttaggtaca ggaatattta taccaacaat ctttccttgc tttattacaa gtgttgcatt
     3181 ttgtaaagtt gtggttgcgt tttgaataat agttgcatta gtaaatgcat aacaatttat
     3241 tcttacatct ttggttccgt ttacaggaaa agtttcttgg gcatttatta tagcaataca
     3301 aaagcatgct acaattagca gttttagttt tgtaaacatc tttaaaatat ttgttaggac
     3361 taaaattaga taaaacagta caaaaaatta taccgttcat aaaattaaaa aaataaaaat
     3421 agctgctaac cgttttatat ttctttagca gctctggcat aggctttttt ttgtttccag
     3481 ttataccatg gctgtggaat ggtttttttc aaaagcgtat ctatatttct cataccaaat
     3541 aaattccaca caccagcaag tttgcctgtt atagaaggtt gtaaggctct taaactcata
     3601 gcaaatccac tatctaaata ctccatgtgg tgccaataac cagcaggcat aaatagagta
     3661 tctccatgtt ctaaaattac ttccattcct tgtgcatttt ttagaatagg aaatttagta
     3721 taatctattt tactatttgc ttcatcataa tagttactaa aatcaacaaa acttaaaact
     3781 tcaaatggtt tacgatataa tttatattgc tctttaaacg gaaataataa tactcttttt
     3841 ctaccaataa actgtgtatg aaaaatatgg cttaaatcta tatcaaaatg catgtgagtt
     3901 atagatgttg caccaccagt gaataacatt ggatattttt ttacaaagcc ttttgttaaa
     3961 tgctctggcc aagtaaaatc atttattagt tgtggggcat gatcaaaaat attaaataaa
     4021 aaaattctcc atcctgcagg ccctgctttt atcatatcaa tatactctcc aaaggttttg
     4081 taatcatctg ctgtattgat aggtgtgtat gcattgcttt taatattatt atacaaagcc
     4141 actttttgac tgcctaccaa tgttttaaaa taatcccaat tccatttact ataagcagac
     4201 cattgctgag ataaactttt tattactaat ggctttgaag taagataata ttttttttta
     4261 aactcttctg ccgaaatatt ttgaacaata tctactggat gtaattgcat ataaaataaa
     4321 attcaaaaat aaataagaat ttattgaaga aaaatataat tatttaaaac ttttataaaa
     4381 ataaatcagt ataaagttta gcacctggta caacagcata tttatcaaag ttggtgatac
     4441 cttctttggc aagtacaatt tcatcaataa atgtgttgcc agtatattca agattttgtt
     4501 tagataaaat ataaaaagct gcgtctgcaa taatttgtgg agtgcgactc atgttagcta
     4561 aagtttcgcc acctaataaa tttcttactg cagcagtatc tatggttgtt tgtggccata
     4621 gtgcgttgct tgcaatgcca aaatttttaa actcttctgc ccaagctaat gccatcatac
     4681 tcatattaaa tttgctcata gtataggcaa tatgagggct aagccattta cttattaaat
     4741 ttactggtgg tgaaagtgta agtatgtgtg gattgtttcc tttttgtaga tagggtaaaa
     4801 aatgctgcac tactaaaaag ctaccacgta catttacact atgcattaag tcaaagcgtt
     4861 tcgcttctgt ttgtgctgta ttggttagtt gaattgcaga agcattgtta attacaatat
     4921 caacacctcc aaatgtttct attgtttgtt ttacagcatt ggctatttgc tcttcatttc
     4981 taacatctgt ttgtactgct aaagctttgc cacctgcagc ttctatttct tttgcagcag
     5041 tataaattgt acctcctagt tttgaatttt cagtagtgct ttttgccgca ataacaatat
     5101 tagctccata acttgcaagc tttaatgcta tggctttacc aatacctctt gaagctcctg
     5161 aaataaaaac tgttttattg ttaaataaca tgcggtaaaa aattattagt ttaatgttgc
     5221 aatatcatct tgtatggaat caggaaaaga agttggcata ttgtttaatt catttatatc
     5281 taacccttct tcctgtattt cttgttctct gttttcattc cattcaataa taaaattatt
     5341 tctcccctcg ccaattaata atttcatgaa tcgttgttgt gtaagttcta acatagataa
     5401 aaaaagaaag atggcatgca ctctactctc acaaacatca aaaacttttt cgaatgaaac
     5461 tgttttttct ttttggataa aatgtaacat gtaatctctg cttccctcca ttgtataatt
     5521 ataccttaca acagtgtgta ctggcttgtt ttgtctttca ttgaggcgtt gcattacttt
     5581 ttcaaaagat ttcattagtt tgaataaagt aatgttttga atttctgttc cttctcctgc
     5641 ttgctctcct atttcattta actctttttg caagttgcct ctttttacca ttaacattct
     5701 caaggcttcc atatctgcca tttttgcgga ggcttcttta aacttcttat actctaatat
     5761 tttatctact aactcttgtc gtggatctat ttctttacca tctgcatcta actctttacg
     5821 tggtataagt agctttgctt ttattctcat tagggtagaa acaaataaaa taaactcgct
     5881 acttaattct atatttaatt tttcctgaga gtgtatatag tttaaaaaat cgttggtaat
     5941 ttttgtaatg ggtatattgt aaatatctaa ctcatctctt tcaataaaaa ataataacaa
     6001 atcaaaaggc ccctcaaagt ggtctaattt tatttggtaa gaagttgtat tcacagcaat
     6061 tttatttatt gcaaattaaa cgtacaatta agatatgaag agcaattaca cagcttaatt
     6121 atccactata aaaaataatt tatacccttt attttaaggg aagaaatact tcagccatca
     6181 tacatcttgc acttcctcca ccatttgttt caatagttgt aaggttggaa tgtaatatat
     6241 ggttgtagct ttgtagttga tttatttgtt gtggcgttaa ggcttggtat gcttgcgatg
     6301 acataatcaa aaacttttct cctttttgat tattaacttg tagcatattg ccagcaaatt
     6361 tgttcatttg ctgtaaggtt attggtacaa tttgtttacc agagctttct atagagtgaa
     6421 taaccgtact tctctcttga tggtcagtaa ttgcatctaa acaaattacc acatatttat
     6481 cagctacaca catcattaca ttggtatgat aaatgggtga gttgtttgag tcattagctt
     6541 taaacaacat tggtgtaaag tgcattttat tgcaaaattc ttctaaaatt gtggcatctg
     6601 ttcttggaga aatacaggca taagctatgt ttttttctct atctaaaacc atgctcccag
     6661 tgccttctaa aaacaagtta cttttttcgt agttggttaa gtctatggtt gtagaaatac
     6721 tgaatttttt agaaatattt tcaagaacgt gtggttttct ttcttgcctt ctattttcgg
     6781 catacatggg gtataacagg atggtgccat tggcatgaaa agaaatccag ttattaggaa
     6841 aaatagaatc gggtgtatgt ggttgtaaag tatcttctat tacagttaca tcaataccgt
     6901 tctgcattaa taactctaca aaattattaa actcattcag tgcattttgc tgtgtgttgc
     6961 tatctgctgc tgcattttga aatgcattat ttactgctgt ttgtgcatta taagtaaaat
     7021 gcacaggctt tatcatcaac aaatgctgtg tagtttgcat aaaatttttt attaaatagt
     7081 ttaccattta tctcttaata aaggcatact catgcaatga gatcctcctc tggctctaga
     7141 taactctgca gatggtatta gtattactgt atcttctaaa ctatgtgggt caataccttt
     7201 ttcaaacatt tcgaacactt gggttgtagt tacaatatta aaccctgcat ttttaaacgt
     7261 ttctgttgtt ttatcatttc tatcataact aattacaacc ccttctttta aggctactac
     7321 attgctagag tctgtccatt gttctctttc atcatgagga aattcgttgt tgccgctata
     7381 aattatttta acatcttcgg gctttacacc aaaatctacc tcacttatat gccttaataa
     7441 tgactctaac cctgcagggg cattacttat gctataatct ttatttttag cataaggttc
     7501 ttttttgttt ttatagaatt gaaaaatttc tacttcttct agctctgttt ttttaggcac
     7561 atgcgataat tttttaagat aagatttctt ggtatcttct tctgctttta agatagtatc
     7621 agaatatctg ccatataaaa cccaaacatt tctttttact tgtgtaaaaa tggtgtctat
     7681 atgcatttgg gctctttttt taggaatttt tactacagaa attttttcaa tttctagatg
     7741 agagttattg aaaatggtat gaataatttc attaattgca ctagccgatg ttctttcgct
     7801 acaacctacc aataaatgtg ttgggtgaat catcataata tcgccaccct caatagtaat
     7861 tattctttgc ttacgttctt cttcttcata caaaaaataa tcactttctt caattacttc
     7921 aattaccttt tctggattat ctctaaataa aaaataaagt gctaaaaatt tagaaattaa
     7981 tgactctctt ttacgagctg ttgttgccat tctgctcagt aaaatatggt ctttcatcat
     8041 tatagcatta tctcttgtaa atataaaatt aggaatgggt ggaaaaatat attgatcctc
     8101 tttggttcca ttttcttctg caggaaaaat gcctgtaata
//
LOCUS       DAOQHP010000052         8133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4536_length_8133_cov_4.050879, whole genome shotgun sequence.
ACCESSION   DAOQHP010000052 DAOQHP010000000
VERSION     DAOQHP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_8133_cov_4.050879"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLY81_02870"
     CDS             <1..605
                     /locus_tag="PLY81_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNF29202.1"
                     /translation="AIQTLAKTTVPENIIQRIEEMAIKDKNSKVRSSAIDFLAKLENK
                     KYRSIFEKSVYDSSYSIAGASLEALEAIDSEAALAITKKIMKEPAKGRLSAAISNVLV
                     KHGGDEAFDFVAENFEKAPFSQAKFETMQALADILVKVTDFKKFIKGVDLIVEFRDSI
                     PEAYKAQITAYINNMVLKTIADKKEAAGDKEKADYVKSKL"
     gene            789..5918
                     /locus_tag="PLY81_02875"
     CDS             789..5918
                     /locus_tag="PLY81_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNF29203.1"
                     /translation="MATKSLPKKLIGIFCLTILLFTVVNKVQSQTTYYVNDGSLTGDI
                     ATTAIGDDLNAGTNTAPFATITKALTLAVSGDLIIADAGTYEENGFTITAGVKLSGVC
                     ASKSIIVVKVSSITLGSASELTRFTITRQSGSDVPSVALATTTGGADMKINNCFFTKC
                     RTAIYINSTTAANALTVSNNDFDDNRTGFVLADATYSNITISNNNIVDNRSFGIIFTA
                     ATTLSNISIANNNITGNLAAGIGIEGSATSGITLTNNWHGTPTVGIYLGVLNGGFSVD
                     AHNVPSSYPYNFTDLNGVAEPNYPKEIGGVGVTAISGATYASSEISFALPSCGVSSDN
                     PVGWYSTIQAAVTASNSGANIFVTSGIYAENVTVNKSLTIVGEDSALVKLDKGDANYT
                     ALGGNGFTITANNVSISKFKIRNYNNGVSNTIDASNISLSQITAIENYGSGFYSNKSI
                     NGLSITNSTFKNNGYKNNIHSGGTRRGIMTQSSSSDIANVTITNNVVTDNSLVGIDIN
                     LNRSTDGINISDNTLARNGDDELGVWLGNNNKDTSAAVVIKNNNITLSNAVRFGIEIK
                     NPSGSGDSTGVGSIFVSSNTITTSAHIGATRDMGGIVVIRRKDGYSGALCINDQPQGV
                     VIYNNTVNVENIHVNANDAYGIVVGGTGHIVYGNTINNTEVSIQLQTQNDNYLTNNSS
                     NQALTNYYFSRDNSKYSCAIVSSNTINMSGAPRLVTDVSTSTATLSGIQNINTGEYFC
                     AIQSAIDFASTNDVINVASGTYTEDLYLDKKLTIRGANFGISPSGPRMPETTLIPKSS
                     DPDPNSPTAVTMLYITATGSGSTIDGFTFDGNNPDSTSGVLINEGSVDVDACEAISSY
                     DGISNVSISNNIIQNINYCGIDFYNYTNGGTATSGNLIANNLFQGIRPSEYGMAILLY
                     NNCYTSITNNTILGVRVGVQTGNFSQTDPGTSHSITGNNIESVARGIWHNLHYNAASK
                     FDISNNNISTHVTGTANRGIMISTLQQTVGVTLSNNSISGAKYGYYLYSNPTTDTVTI
                     TGGFISSTNGIVADNYLGYNANAESSNYKLDGVSILSNDTAIIIRDDAANTNNATVSM
                     VLKNNIYANSMPSNIALAILGSDASALVTNAGGVTALHLLGNIVNNSGNSLDATNADL
                     EINGSTANISGGATVRNLISNTTTSTVIASGIGNTINITNSLTPGDGPLTTNNNLVLK
                     STATGTASILQGDVAGTYIVGDVTVEKYIPAKASRKWLFVTSPITGTLSNTWQQQIHL
                     TGAGTGGTVCPTLTAHTNGYDPTISSASNIYTYNASATAGMRWQKPATTNADNLTAGK
                     GFRLNVRGNRSLGCDLINGNPAFLAPTATTLSATGNINLSNKNAGDFTINYSNTDPLN
                     YVFIGNPYPSAISFSALYISNMANINNTYAIYIPSNAAGVYSYWDGSTFTGGSGYDNL
                     TGNIIANGQAIFVQAIGSGDLNLNFSESHKTDNTTAGYFRPNSNTQSFKVNYLNDNNN
                     KIDEIVVRFANDATISNNTIGKLDIPSMNHDTYITSLKGATHTSVQTRDINTISNDEV
                     WLNIGATKSGNYKLNFSDYDQLIGTDIYLIDHLANITQDVKQNPEYVFSVDINNAATK
                     GSSRFSVVFSQRVQPVVTQSIKMYPNPARNKVTIMLPTSADNNQHYSINVTDITGKTV
                     KVYKAVKGTQQLNIDKLTTGSYLVEITDSKGSRITEKLIKQ"
     gene            6067..6258
                     /locus_tag="PLY81_02880"
     CDS             6067..6258
                     /locus_tag="PLY81_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29204.1"
                     /translation="MKTKFLRVIGLLAIVMLIAMPVIAQDNFPDPEDDIPIDGGLTLL
                     LAAGAGYGAKKLRDKKRKM"
     gene            complement(6628..>8133)
                     /locus_tag="PLY81_02885"
     CDS             complement(6628..>8133)
                     /locus_tag="PLY81_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29205.1"
                     /translation="YFMYKAEKNTIQILLLCIVLIGVYYYRPTNLYFLGDDFFHIPES
                     VKNIWIQQNSVRPIGNISLLIDYFFSNSNALGYHITNLLIHTLNSFLVAVLSKKLFNY
                     FLILPSTNWLTWIVGIFFFVYPFHSEAVFWIIGRSGSLGALFFVAALICFLLRERSLL
                     YSIASILFFQLAIFSYESSWLFPLFICLIYFFKQGLKKILRSDIIYFALVWLLLLINL
                     FLISKATLNLFSNYEASAFIHFNIKLLFQNFIKLFARTLLPPFFSNLYFIVALIVTFL
                     ICVLLIILFFKKKANTKFFYLLSVLWLLSYLPYLSIGIDTHGTEGERYLYLPSVFFCL
                     WLVYILYSISNTRHFFISIILLMSFQLYFLQKSRAYYVKASDITKTTINQIKLLESKE
                     KLYFDNLPQYNKGAVVFRRGLYNAIRWLYPNQKYDVVVVSIDDSDIKPRDKHINVFEV
                     LYSDKNFPKAVTAIKEWNGIEQNKIRGGGIIFNPKKDAWFSFNEWSLTIVR"
BASE COUNT         3098 a         1224 c         1310 g         2501 t
ORIGIN      
        1 aagctattca aacattagca aaaacaactg taccagaaaa tattatacaa agaatagagg
       61 aaatggcaat aaaagataag aatagtaaag taagatccag tgcaattgac tttctagcaa
      121 aattagaaaa taaaaaatac agaagcattt ttgaaaaaag tgtatatgat tcatcttaca
      181 gtattgctgg tgcaagttta gaagcattag aagccattga tagcgaagct gcattagcta
      241 ttacaaaaaa aataatgaaa gaaccagcta aaggacgatt atctgctgcc atttccaatg
      301 tgttagttaa acatggtggc gatgaagcat ttgattttgt agctgaaaat tttgaaaaag
      361 cccccttttc tcaagctaaa tttgaaacca tgcaggcttt agctgatatt ttggttaaag
      421 taacagattt taaaaaattt ataaaaggcg ttgatttaat tgttgagttt agagactcaa
      481 taccagaagc ctacaaagca caaattactg catatattaa caatatggtt cttaaaacca
      541 ttgcagataa aaaagaagcg gcaggtgata aagaaaaggc agattacgta aaaagcaaat
      601 tgtaaggtta attcttaatt attttattac tcaagctatc acagtaagtg gtagcttttt
      661 tattatttat tttttttaat tattaaaagt ttatatattt gagttccccc cttaaaactt
      721 atcaacttaa aatcgagtat cctaaactgt ttttttaaaa aaaaattatt tatttaaaac
      781 tacaatttat ggcaactaaa tctttaccca aaaaattaat tggtattttt tgtttaacca
      841 ttttactatt tacggttgta aataaggtac aaagccaaac aacttattat gtaaatgatg
      901 gttccttaac aggagatatt gcaacaactg caattggcga tgacttaaat gcaggcacca
      961 atactgcacc ttttgccacc attacaaaag cactaaccct agctgttagt ggtgatttaa
     1021 taattgcaga tgctggcacc tacgaggaaa atggttttac cattacagca ggtgtaaaac
     1081 taagtggtgt atgtgcatct aaaagtatta tagtagttaa agttagttca attacattag
     1141 gctccgcctc agaactaaca cgttttacca tcacaaggca atcaggaagt gatgtaccaa
     1201 gtgttgcatt agctacaaca actggtggtg cagatatgaa aataaataat tgctttttta
     1261 caaaatgcag aactgcaatt tatataaact ctacaaccgc agcaaatgct ttaactgtaa
     1321 gcaataacga ttttgatgat aatagaactg gttttgtttt agcagatgct acttattcta
     1381 atattactat atctaataat aatattgtag ataacagaag ttttggaata atttttaccg
     1441 ctgccactac cctttcaaat atttctattg caaataataa tattacagga aatttagctg
     1501 caggaattgg tatagaaggc agtgcaacta gtggtattac acttacaaac aattggcacg
     1561 gaacaccaac agttggtatt tacctaggtg ttttaaatgg tggctttagt gtagatgctc
     1621 ataatgttcc ttcttcatat ccatataatt ttacagattt aaatggggtt gccgaaccaa
     1681 attatccaaa agaaattggt ggtgttggtg taactgcaat atctggtgct acgtatgcat
     1741 catctgaaat tagctttgca ttaccttctt gtggagtaag ttcagataat ccagttggtt
     1801 ggtattcaac tatacaagca gcagttacag cctctaactc cggagcaaat atttttgtaa
     1861 catctggtat ttatgcggag aatgttactg taaataaatc tttaactatt gttggagaag
     1921 atagtgcttt agtaaaacta gataaaggcg atgctaacta tactgcatta ggtggtaatg
     1981 ggtttactat tactgcaaat aatgtttcta tttctaaatt taaaattcgc aactacaata
     2041 atggagtttc taatactata gatgcaagta atatttcttt atctcaaatt actgcaattg
     2101 aaaactatgg atctggtttt tacagtaata aatctattaa tggattaagt attaccaatt
     2161 caacctttaa aaacaatggc tataaaaata atattcatag tggaggtaca agaagaggta
     2221 taatgacaca atctagcagt agcgatatag ctaatgtgac tattaccaat aatgtagtta
     2281 cagacaatag cttggttggt atagatatta acttaaacag atctacagat ggtatcaaca
     2341 tctctgataa tactttagca agaaatggag atgatgaact tggtgtatgg ttgggtaata
     2401 acaataaaga taccagtgct gcagtggtta ttaaaaataa caatataaca ctaagtaatg
     2461 ctgtaaggtt tggcattgaa attaaaaacc catctggtag tggagactct acaggtgtag
     2521 gaagtatttt tgtttctagc aacactatta caacaagtgc acatattggt gcaactagag
     2581 atatgggagg tatagtagta attagaagaa aagatggtta ttctggtgca ctatgtatta
     2641 atgaccaacc acaaggagta gttatttata ataataccgt taatgtagaa aacatacatg
     2701 taaatgcaaa cgacgcttat ggtatagtag ttggaggaac aggtcatatt gtatatggaa
     2761 atacaattaa caatacagaa gtctcgatac aacttcaaac ccaaaacgat aattatttaa
     2821 caaacaatag ttccaatcaa gcattaacca actactattt tagtagagat aattcaaaat
     2881 attcttgtgc tatagtttca agcaatacaa ttaatatgtc tggtgctcca aggttggtaa
     2941 cagatgtttc tacaagtaca gcaacattat ccggaataca aaatataaat acaggcgaat
     3001 atttttgtgc tattcaatct gctattgact ttgcatctac caatgatgtt ataaatgtgg
     3061 caagtggaac ttataccgaa gatttatatt tagataagaa actaaccatt aggggtgcaa
     3121 attttggaat aagtccttct ggcccaagaa tgccagaaac tactttaatt cctaaaagtt
     3181 cagatcctga tccaaatagt cctactgcag ttactatgct atatattaca gcaactggta
     3241 gtggctctac aattgatgga tttacttttg atggaaataa tccggatagc actagtggtg
     3301 ttcttattaa tgaaggaagt gtagatgtag atgcttgtga agcgatttca tcatacgacg
     3361 gaatttcgaa tgttagtatt tctaataaca ttatacaaaa tatcaactat tgcggaattg
     3421 atttttataa ctatactaat ggtggtactg caacatcagg aaatttaata gctaataatt
     3481 tatttcaagg tattagacct tcagaatacg gaatggcaat tttactttat aacaactgct
     3541 atacaagtat aacaaacaat actatattgg gtgtaagagt tggcgttcaa acaggtaatt
     3601 tttctcagac ggaccctgga accagtcatt ctattacagg aaacaatatt gaaagcgttg
     3661 ccagaggtat ttggcataat ctacattata atgcagcttc caaatttgac atctcaaaca
     3721 ataacatatc aacacatgtt acaggtacag ccaatagggg tattatgatt tctacattac
     3781 agcaaaccgt tggagttaca cttagtaata attctataag tggagcaaag tatggatatt
     3841 atttgtatag taaccctaca acagatacag ttactataac aggaggtttt atctcttcta
     3901 caaatggaat tgtcgcagat aattatttag gatataatgc aaatgcagaa tcaagtaact
     3961 acaaacttga tggtgttagt attttatcaa atgacactgc aataataata agagacgatg
     4021 cggcaaacac taataatgca actgtttcta tggtattgaa aaataatatt tacgctaata
     4081 gcatgccgtc caatattgca ttagcaatat taggaagcga tgcctccgca ttagtaacaa
     4141 atgcaggagg ggttactgcc ttacatttat taggaaacat tgtaaataat agtggaaatt
     4201 cgttagatgc aaccaatgca gatttagaaa taaatggttc aacagcaaac atctctggtg
     4261 gtgcaactgt tagaaacttg attagcaata caactacaag tactgtaata gcttcaggta
     4321 ttggaaatac gattaatatt actaattcct taacaccagg tgatggccca cttaccacca
     4381 ataataattt agtattaaaa tcaacagcaa ctggtacagc ttctattttg caaggcgatg
     4441 tagcgggcac ctatatagta ggtgatgtaa cagtagaaaa atatatacca gccaaagcaa
     4501 gtagaaaatg gctatttgtt acaagcccaa taactggcac attaagtaat acatggcagc
     4561 aacaaataca tttaacaggt gctggcacag gaggaactgt ttgccctaca cttactgcac
     4621 atactaatgg ttatgatcct actattagta gtgcatctaa tatttatacc tacaatgcct
     4681 ctgcaactgc tggtatgcgt tggcaaaaac ctgcaaccac caatgccgat aacttaactg
     4741 caggaaaagg ctttagatta aatgtacgtg gcaacagaag cttaggctgc gatttaataa
     4801 atggtaatcc agcattttta gcaccaactg ccactacttt aagtgcaact ggtaatatta
     4861 atttaagcaa taaaaatgca ggagatttta caatcaatta ctccaatacc gatcctctta
     4921 attatgtatt tattggcaac ccttatccta gtgctattag ctttagtgca ttgtatatta
     4981 gcaatatggc caatataaat aatacctatg ccatatatat accatccaat gctgctggtg
     5041 tatatagcta ttgggatggt agtacattta ctggaggttc tggatacgat aatttaacag
     5101 gcaatattat tgccaatggt caggcaatat ttgtacaagc aattgggtct ggtgatttaa
     5161 acctaaactt ttctgaatca cataaaacag ataatactac tgctggatat tttcgaccta
     5221 attcaaatac acaaagtttt aaagtaaatt atttaaatga taataataat aaaatagatg
     5281 aaatagtggt tcgttttgca aatgatgcaa ccatcagtaa taataccatt ggtaaattag
     5341 atataccaag tatgaaccat gacacttata taacaagctt aaaaggtgca acacatacat
     5401 cagtacaaac aagagacatt aatactatta gcaacgatga ggtttggtta aatataggtg
     5461 caaccaaaag cggcaactac aagttaaact ttagcgatta tgaccaacta attggaacag
     5521 atatatactt aatagaccat ttagccaata ttacacaaga tgtaaaacaa aacccagagt
     5581 atgtatttag tgtagatatt aataatgcag ctactaaagg aagtagcagg tttagtgtag
     5641 tatttagcca aagggtacaa cctgtggtaa cccaaagcat taaaatgtat cctaaccctg
     5701 caagaaataa agttaccata atgttaccta caagtgctga taacaaccaa cattatagca
     5761 taaacgtaac tgatattaca ggtaaaacag taaaggtata taaggcagtg aaaggtacac
     5821 aacaattaaa catagataag ttaacaactg gtagttattt agtggaaata acagatagca
     5881 aaggcagcag aataacagaa aaattaatca aacaataata tataatgtca tcctgagcga
     5941 agcgaaggat ctgctgaaaa atgtactaat gcacaagcag atgtttcgct tacgctcaaa
     6001 atgacaatag gaagaacaca aacagattct tcatttcgtt ctgaaaagta taaaaagtaa
     6061 aaaaatatga aaacaaaatt tttaagagta ataggcttat tagcaatagt aatgttaata
     6121 gcaatgccag ttatagcaca agataatttt ccagatcctg aagatgatat accaattgat
     6181 ggaggattaa ctttattact agcagcaggt gcaggctatg gtgctaaaaa attaagagac
     6241 aaaaaaagaa aaatgtagga gattgtaata tataataatt actccttaaa aggtattgca
     6301 gcaatgcaat acctttttta taatatcaat ataaacttat taaaacaaat agatttttat
     6361 acagattact tgagtggtaa gctaaataac attaccccaa ttataggata atgtataatt
     6421 aatgaaagta gcaaagtagc cacacatgta aaccctaata gattagatat tcctatcagt
     6481 ttacataatg gtaaagaatt tgtaataaaa aattgtagtt ttaaaaatgg tttaaatact
     6541 gatagattta gaattactta tttaactaga actgaaggat aatacattta aaagttaatt
     6601 tagggcaatt catattccta tattagatta tcttacaata gttaaagacc attcattaaa
     6661 actaaaccat gcatcttttt taggattaaa aataattcca ccacctctta ttttattttg
     6721 ttcaatacca ttccattctt taatggcagt aactgcttta ggaaagtttt tatcgctata
     6781 caatacttca aaaacattta tatgcttatc tcttggttta atatcactat catcaatact
     6841 aactactaca acatcatatt tttgattagg atataaccat ctaatagcgt tgtaaagccc
     6901 tcttctaaaa acaacagccc ctttattgta ttgtggtaaa ttatcgaaat acaatttttc
     6961 tttgctttct aataatttaa tctggtttat tgttgtttta gttatatcgc ttgcttttac
     7021 ataatatgct ctactttttt gtaaaaaata aagctgaaag ctcattagta aaataataga
     7081 aataaaaaaa tgccttgtat tgcttatact gtataagata taaacaagcc ataaacaaaa
     7141 aaatacagat ggtaaatata aatacctttc accttctgtt ccatgggtat caataccaat
     7201 agacaagtat ggaaggtaac tcaacagcca taatacactt aacaaataaa agaacttagt
     7261 attcgctttc ttcttaaaaa ataaaataat taataaaaca caaataagaa aggtaacaat
     7321 taaagcaaca ataaaataaa gattactaaa aaagggtggt aacaatgttc tagcaaatag
     7381 ttttataaaa ttttgaaata atagttttat attaaaatgt ataaatgccg atgcttcgta
     7441 attactaaaa agattaagtg ttgctttaga aatgaggaat aagttaatca ataacaaaag
     7501 ccaaacaaga gcaaagtaaa taatatcact tcttaatatt tttttaagcc cttgcttaaa
     7561 aaaataaatt aagcaaataa ataacggaaa cagccaagac gattcgtaac taaaaattgc
     7621 aagttgaaaa aatagtatag aagcaataga atataataat gaacgctctc ttaagagaaa
     7681 gcaaattaat gcagcaacaa aaaacaatgc tcctaaacta ccagatctgc caataatcca
     7741 aaacacagct tcgctatgaa atggatatac aaaaaagaaa attccaacta tccaagtaag
     7801 ccagtttgta gagggtaaaa ttaaaaaata attaaataat tttttactta gtacagctac
     7861 taaaaagcta tttaaggtat gaataagtaa attagtaata tgatagccca aggcattact
     7921 gttgctaaaa aaataatcaa taagaagaga gatattacca attggcctta cagagttttg
     7981 ttgaatccaa atattcttta cactctctgg tatgtgaaaa aaatcatcac ctaaaaaata
     8041 taaattagtt ggcctgtaat aataaacacc tatcaatact atgcaaagca ataaaatttg
     8101 aatagtattt ttttctgctt tatacataaa ata
//
LOCUS       DAOQHP010000053         7991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4660_length_7991_cov_4.118070, whole genome shotgun sequence.
ACCESSION   DAOQHP010000053 DAOQHP010000000
VERSION     DAOQHP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_7991_cov_4.118070"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLY81_02890"
     CDS             complement(<1..477)
                     /locus_tag="PLY81_02890"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNF29206.1"
                     /translation="MPVIPPFNLKKWIDENRDLLKPPVGNSCVYKDAENFIVMIVGGP
                     NARKDYHFNETEELFYQIEGDITVKIIDEGVFKNIHIKEGEMFLLPPKTPHSPQRGPN
                     TVGLVIEKKRESEDDGFLWFCENCGNKLYEETLHVDDIVKQLPPVMNRYWESIMHRT"
     gene            592..1377
                     /locus_tag="PLY81_02895"
     CDS             592..1377
                     /locus_tag="PLY81_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF29207.1"
                     /translation="MNISLENKTAIICGSTQGIGLATAKELSNLGATCILMARNQTAL
                     IEALKQLNYSFNQPHNYLVADFDNPSTVKSAMVEFIKKNTVHILVNNSGGPNAGQITD
                     ATEQQFLDTFNRHLICNHILTKAVINGMKEAGFGRIINIISTSVKIPLPNLGVSNTIR
                     GAVASWSKTMANELGQFNITVNNVLPGATATGRLKNIIENNASKKNITLEEIENEMQK
                     EIPMKRFAQPEEIANAVAFLASPAASYINGVSIPVDGGRTGSL"
     gene            1438..2895
                     /locus_tag="PLY81_02900"
     CDS             1438..2895
                     /locus_tag="PLY81_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNF29208.1"
                     /translation="MNLEIPYYIENYIGGNLVAPLSSQFIDCVNPATAEVFAQIPNSN
                     EKDIELAVQAATIAKESWGNTTLEERFKILNRIADLIDENIDAFALAETNDNGKPLWL
                     SKKVDIPRASANFRFFATGIMHFASESHSMQGKAINYTLRQPIGIVGCISPWNLPLYL
                     FTWKIAPALAAGNCVIAKPSEVTPITAFLLSKICIIAGLPAGVLNIVHGLGNSCGEAI
                     VKHKKIKAISFTGSTRAGEQIASIAAPMFKKLSLELGGKNPNIIFADCNWDKMMKTTI
                     QSSFSNQGQICLCGSRILIEESIYQKFKSEFIEKVKSLTVGDPLHENSKQGAVVSKSH
                     FDKVLNCIEIAKNEGGKILLGGNAVKLEGRCANGYFIEPTIIEGLDANCTTNMEEIFG
                     PIVTLQTFKTLEEALQLANTSNYGLSATIWTQDISKANYIAAKTESGILWVNCWLVRD
                     LRTPFGGNKNSGIGREGGWEALKFFTETKNVCIEF"
     gene            2965..3384
                     /locus_tag="PLY81_02905"
     CDS             2965..3384
                     /locus_tag="PLY81_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNF29209.1"
                     /translation="MENKVIHTELAAKPLGSYPHAVKVGNLLFLSGIGSRKPEDNSIP
                     GLQLDSCNNIIQYDIEAEVHQVMANVKAVLEASGSCWKNIVDVTVFLTNMKKDFPIYN
                     KLYGEYFKDVQACRTTVEVKSLPTPIAIELKVIATIN"
     gene            3392..4663
                     /gene="kynU"
                     /locus_tag="PLY81_02910"
     CDS             3392..4663
                     /gene="kynU"
                     /locus_tag="PLY81_02910"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNF29210.1"
                     /translation="MIYQNTLAFAKNLDEQDEIKDFRTKFFIPQHNGRDTVYLTGNSL
                     GLQPKSTAAYIQQELNDWANLGVEGHFHAKNPWMPYHEIFPKQLSKIVGALEEEVVVM
                     NQLTVNLHLLMVSFYQPTAQRYKIICEAKAFPSDQYAIESQVRFHGLNYEDAVIEVEP
                     REGEHTIREEDILNTIEQNKDSVALVLFGGVNYYTGQVFNIKNITEASHKVGALVGFD
                     FAHATGNINLQLHNWNVDFACWCSYKYLNSGPGGVAGAFIHQKHITNKKLKRFAGWWG
                     YNKETRFKMERGFIPIPTAEGWQLSNAPVLSMAAHKAALDIFDEVGMDRLHKKRKLLS
                     SFVLFILDDINQKSNTQTIEIITPRNENEIGCQVSMLMLKNGKQIFNALMQQGVIADW
                     REPNVIRIAPVPLYNSFEDVWKFGDIIKQIV"
     gene            complement(4853..5416)
                     /gene="ruvC"
                     /locus_tag="PLY81_02915"
     CDS             complement(4853..5416)
                     /gene="ruvC"
                     /locus_tag="PLY81_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNF29211.1"
                     /translation="MTTKPHIILGIDPGTQLLGFALLKVEGNKQQVLLMDVLKLTSHK
                     DIYVRLEKIHSKVTELIKLYQPKTFAIEAPFFGKNVQSMLKLGRAQGVAIAAAIHAGL
                     DVTEYSPKKVKQSITGNGNADKEQIWKMLQRVLNIDEKPQYYDATDALAVALCHCYQL
                     SSSLNLKDKKFNGWNDFIQKNPNRFKQ"
     gene            complement(5418..5984)
                     /locus_tag="PLY81_02920"
     CDS             complement(5418..5984)
                     /locus_tag="PLY81_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29212.1"
                     /translation="MKKFFYLLVLMLIIKTTNAQETSIIVGGETKPAISRDVNEKESD
                     VIDALKYYFQQKGVKKFKKSKGIYTVKGIKINEITNDNLDLFFSIDRKGKKKDKLTTV
                     SMAVHTTERQFVGESTHEQIKQKLPAFMDLLPTIVSDYHKKLEADRLQKEADDARKEK
                     EKLEKQAAKQAAKAAKLQKEADQAKTEK"
     gene            6080..7579
                     /locus_tag="PLY81_02925"
     CDS             6080..7579
                     /locus_tag="PLY81_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF29213.1"
                     /translation="MSGIKKLAGQTMWYGLSTIAAKFINYILTPYITFKFTNAQYGEM
                     SIIYSFIPFMNIIFTYGMETAYFRYANTYDAKKIYNTISISIIISSIIFSVIMLLMAK
                     PLTTLLTIEADNNYLVLAVCIIFLDALSTLPFAKLRQENRPLKFAFIRITSILINVFC
                     IYFFLSLCPNWLKENPTHWCSKFYQPNFAVGYILIANIVQSLFTLLLLSKEFFSFSLN
                     FDTKLWKEMMLYSLPLIIAGFGGMINETFDRIMLSWWAPVQTEMAAKSEVGIYAACYK
                     LSILITLSVQAFRMGAEPFFFKKSKDADAKKTYARVMNFFVITVCFMFLLVALYLDIW
                     KHFIQNANMWQGLHIVPILLLANIFLGIYYNLSIWYKLVNKTNVGALITIFGVVITLV
                     INYFFIPYYSYTACAWATFACYGSMMVLSYLWGQKEYPVPYEWVKLCSYIFYVVALYF
                     IHSILIKHFTTFWSSFTIASLLVLLFSFIVFIKEKSELKNLPIFNKLIK"
     gene            7651..>7991
                     /locus_tag="PLY81_02930"
     CDS             7651..>7991
                     /locus_tag="PLY81_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF29214.1"
                     /translation="MRVIIVGGGFAGLKLARLLNNKNGIDITLLDKYNFHQFQPLFYQ
                     VATAGLDASNISFPLRKVFQKSKNIRIRLAEVQKIDSKENKIVTDIGIFEYDYLVLAT
                     GANTNFFGNEL"
BASE COUNT         2736 a         1152 c         1252 g         2851 t
ORIGIN      
        1 agttctatgc attatacttt cccagtacct attcattaca ggtggtaact gttttacaat
       61 atcatctaca tgtaaagttt cttcgtacaa tttatttcca cagttttcac aaaaccataa
      121 aaaaccatca tcttcagatt ctcttttttt ctcaattact aaaccaacag tgttaggtcc
      181 tctttgtggg ctatgtggtg ttttaggagg taataaaaac atctctcctt ctttaatatg
      241 tatattttta aatacccctt catcaataat ttttactgtt atatcacctt ctatttgata
      301 aaataattct tctgtttcgt taaagtgata atcctttctt gcatttggtc cacctacaat
      361 cattacaata aaattttctg catctttata tacgcaagaa ttgccaactg gtggttttaa
      421 taaatctcta ttttcatcaa tccatttttt taaattgaag ggaggaatta ctggcatgtt
      481 actatttttt aagtttatga attaattagc ttcaaaagaa tttattaaga aacgtttaaa
      541 ggatacactt tctttttact acttttgaaa attaaattat caattacaaa gatgaatatt
      601 tctcttgaaa ataaaactgc aattatttgt ggcagcacac aaggcattgg tttagcaact
      661 gccaaagagt tgtctaattt aggagctact tgtattttaa tggctagaaa tcaaactgca
      721 ttgattgaag cactaaaaca attgaactat tcatttaacc aaccacacaa ttatttggtt
      781 gccgattttg ataacccttc tacagtgaaa agtgctatgg tggaatttat taaaaaaaat
      841 acagttcata ttttagttaa taattctgga ggccccaatg ctgggcaaat tacagatgca
      901 acagaacaac agtttttaga tacttttaat cgtcatctta tttgtaatca tatattaacc
      961 aaagcggtaa ttaatggaat gaaagaagct ggttttggaa gaatcattaa tataatttca
     1021 acctctgtaa aaattccctt acctaattta ggagtttcta atactatacg tggtgctgta
     1081 gctagttgga gtaaaacaat ggctaacgaa ttaggccaat ttaatataac agtaaataat
     1141 gttttacctg gtgctactgc aactggtaga ttaaaaaata taattgaaaa taacgcaagc
     1201 aaaaaaaata ttactttaga agaaattgaa aatgaaatgc aaaaagaaat tccgatgaag
     1261 cgttttgcac aaccagaaga gatagctaat gctgtagcat ttttagcatc gcctgcagct
     1321 tcttatatta atggtgttag tattccagta gatggcggta gaactggatc tttataaaag
     1381 aatttttttc taaaaaagca attaagtaag caaccataat ttaatctcta tctacaaatg
     1441 aatcttgaaa taccttatta catagaaaac tatattggtg gcaatttggt tgcaccatta
     1501 agtagtcagt ttatagattg cgttaatccg gctactgccg aagtttttgc acaaattcca
     1561 aatagcaacg aaaaagatat tgaattagca gtgcaagcag ctacaattgc taaagaaagt
     1621 tggggtaata caactttaga agaacgtttt aaaatcttaa atagaattgc agatttaatt
     1681 gatgaaaaca ttgatgcttt tgcattagcc gaaacaaatg ataatggtaa accattatgg
     1741 cttagtaaaa aggtggatat tcctagggca tcggctaatt tccgtttttt tgctacaggc
     1801 attatgcact ttgcttcaga aagccattcc atgcaaggaa aagctattaa ctatacatta
     1861 cgccaaccaa ttggaattgt tggttgtatt tctccatgga atttaccttt atatttattt
     1921 acctggaaaa ttgcacctgc tcttgcagca ggtaattgtg ttatagcaaa accaagcgaa
     1981 gtaaccccta ttacagcttt tttactttct aaaatttgta tcatagctgg tttacctgct
     2041 ggcgtattaa atattgtaca tggtttaggc aatagctgtg gcgaagctat tgtaaagcat
     2101 aaaaaaatta aagctatttc ttttacagga agcaccagag caggagaaca aattgcttca
     2161 atagctgcac ctatgtttaa aaaactttct ttagagttag gtggtaaaaa tcctaatatc
     2221 atttttgccg attgtaattg ggataaaatg atgaaaacaa caatacaatc ctcttttagt
     2281 aaccagggac aaatatgttt atgtgggagc agaattttaa ttgaagaaag catctatcaa
     2341 aaattcaaat cggaatttat tgaaaaagta aagagcctta cagttggaga tcctttacat
     2401 gaaaattcaa aacaaggtgc tgttgttagc aaatctcatt ttgataaggt tttgaattgt
     2461 attgaaattg caaaaaatga aggtggcaaa attttattag gaggtaatgc agtaaaacta
     2521 gaaggaagat gtgccaatgg atattttatt gaaccaacaa ttattgaagg gttagatgcc
     2581 aattgtacaa caaatatgga agaaattttt ggccctatag ttactttaca aacttttaaa
     2641 actctggaag aggctctgca attagccaac acaagtaact atggtttatc tgcaactatt
     2701 tggactcaag atatttctaa agctaattat attgctgcaa aaacagagag tggaatttta
     2761 tgggttaatt gttggttagt tagagattta agaacacctt ttggtggtaa taaaaatagt
     2821 ggaataggaa gagaaggcgg ttgggaagct ttaaagtttt ttacagaaac taaaaatgtt
     2881 tgtattgaat tttaatactc ttttttctta gtttatttca attaaagcat actttatttt
     2941 caatacttgt aatttaaaaa taaaatggag aacaaagtta ttcatacgga attagctgca
     3001 aaacccttag gctcttatcc tcatgcagta aaggttggca accttttatt cctttctgga
     3061 attggctcaa gaaaaccaga agataattca ataccaggtt tgcagttaga tagctgcaat
     3121 aacattattc aatatgatat tgaagctgaa gtgcatcaag taatggcaaa tgtaaaagca
     3181 gttttagaag ctagtggtag ttgttggaag aatattgtag atgttactgt ttttttaacc
     3241 aatatgaaaa aagattttcc tatttataat aaactttatg gcgaatattt taaagatgtt
     3301 caagcttgtc gtaccacagt agaagtaaaa agtttaccaa caccaatagc aatagaatta
     3361 aaagttattg ctacaataaa ctaactttaa aatgatttat caaaatacat tagcatttgc
     3421 aaaaaattta gatgagcaag atgaaataaa agattttaga actaaatttt ttattccaca
     3481 gcataatggt agagatactg tttatctaac aggcaattca ttaggtcttc aaccaaaatc
     3541 aacagcagct tatattcaac aagaattaaa tgattgggct aatttaggag ttgaaggaca
     3601 ttttcatgcc aaaaatccat ggatgcctta ccatgaaatt tttcctaaac aattatcaaa
     3661 aattgtaggt gcattagaag aagaagttgt ggtaatgaac caacttacag ttaatcttca
     3721 tttattaatg gtaagttttt atcaaccaac agcccagcga tataaaataa tttgcgaagc
     3781 aaaagctttt ccatccgatc aatatgctat tgaaagccaa gtaaggtttc atgggcttaa
     3841 ttacgaagat gcagttattg aagtagaacc aagagaagga gaacacacta taagagaaga
     3901 agatatttta aacacaatcg agcaaaataa agacagtgtg gctttagtat tatttggagg
     3961 agtaaattat tataccggac aagtttttaa tattaaaaat attactgagg caagccataa
     4021 agtaggtgca ttggttggat ttgattttgc ccatgctaca ggaaatatta acttacaact
     4081 gcataattgg aatgtagatt ttgcgtgttg gtgttcttat aaatatttaa actcaggccc
     4141 tggaggtgtt gcaggagctt ttatacatca aaaacatata acaaataaaa aattaaaaag
     4201 atttgccggt tggtggggat ataataaaga aacacgtttt aaaatggaac gtggttttat
     4261 accaatacca actgccgaag gatggcaatt aagtaatgca cctgttttaa gcatggctgc
     4321 tcataaagca gccttagata tttttgacga agttggtatg gataggttac ataaaaaaag
     4381 aaaactatta agcagttttg ttttatttat tttagacgat atcaaccaaa aaagcaatac
     4441 ccaaaccatt gaaattatta cgcctagaaa tgagaatgaa ataggttgcc aagtctctat
     4501 gctaatgcta aaaaacggaa aacaaatatt taatgcttta atgcagcaag gagtaattgc
     4561 agattggaga gaacctaatg taattagaat tgcacctgta ccactttaca actcttttga
     4621 agatgtttgg aaatttggag atataataaa gcaaattgta taattctgta aaaaattaaa
     4681 ggaggcagcg aagaatcgta gcctcctttt caaccctaaa cccttaaaaa actgatgtaa
     4741 tattacaaaa aattatacca atctttaaat gtacatgggc tattggggct atacttcggt
     4801 aaattgtata tattaatagg tgaattaata ttttttaacg atacatggtt tatcattgct
     4861 taaatctatt tggatttttt tgaataaaat cattccaacc attaaacttt ttatccttca
     4921 aatttaaact acttgatagt tgataacaat gacaaagagc aactgctaaa gcatctgtag
     4981 catcataata ttgaggcttt tcatcaatat tcaaaactcg ttgcaacatt ttccaaattt
     5041 gttctttatc tgcattgcca ttacctgtaa tagattgttt tactttttta ggagaatatt
     5101 ctgtaacatc taaaccagca tgaattgcag cagctattgc aacgccttgt gccctaccaa
     5161 gttttaacat actttgtacg ttcttaccaa aaaaaggggc ttctatggca aaagtttttg
     5221 gttgatataa cttgattaat tctgtaacct tagaatgaat tttttctaac cttacataaa
     5281 tatctttatg agaagttagt tttaaaacat ccattaataa aacttgttgt ttatttcctt
     5341 ctacttttaa taaagcaaac cctagtaact gtgtaccagg gtctatacca agaataatat
     5401 gtggctttgt tgtcaaatta tttttcagtt tttgcttggt ctgcttcctt ttgcaatttt
     5461 gctgcttttg ctgcttgttt ggcagcttgc ttttctaatt tttctttttc ttttcttgcg
     5521 tcatctgctt ctttttgcaa tctatctgct tctaattttt tatgataatc tgatacaatt
     5581 gttggtaaca aatccataaa agcaggtaat ttttgtttaa tttgttcatg tgtactttca
     5641 cctacaaatt gcctttctgt agtatgtact gccattgaaa ctgtagttaa tttatctttc
     5701 tttttacctt tcctatcaat actaaaaaat aaatctaggt tgtcattggt aatctcatta
     5761 atttttattc ctttaactgt ataaatgcct tttgattttt taaatttttt aactcctttt
     5821 tgttgaaaat aatactttaa tgcatcaatt acatccgatt ctttttcatt tacatctcta
     5881 ctaattgctg gctttgtttc accacccaca attatggaag tttcctgtgc attggttgtt
     5941 tttataatta gcattaacac taacaagtaa aaaaactttt tcattgattc ttaaatttag
     6001 tttaataatt atttcaaaat taataacata atttgtaata ccataatcat tactattttt
     6061 gatacataaa ttttaactat tgagtggaat aaaaaaactt gcaggtcaaa caatgtggta
     6121 cggattaagt accatagctg ccaagtttat taattacata cttacaccgt atattacctt
     6181 taagtttacc aacgcacagt atggtgaaat gagtattata tattctttca ttccttttat
     6241 gaatattatt tttacttacg gcatggaaac agcttatttt aggtatgcca atacctatga
     6301 tgctaaaaag atttacaata ctatttcaat ttcaattatt atttcttcta ttattttcag
     6361 tgtaataatg ctattaatgg caaaaccatt aaccacatta ttaacaattg aagccgataa
     6421 taattattta gttttagccg tttgtattat ttttttagat gccttaagta cattaccatt
     6481 tgctaaacta aggcaagaaa acaggccact aaagtttgca tttattagaa ttaccagtat
     6541 attaattaat gtattttgta tctacttttt tttgagcctt tgcccaaact ggttaaaaga
     6601 aaatcctact cattggtgta gtaagtttta ccaaccaaat tttgcagttg ggtatatttt
     6661 aattgcaaac attgtccagt ctctttttac attattatta ttatctaaag agttttttag
     6721 ttttagttta aactttgata ccaagctttg gaaagaaatg atgctgtata gtttaccatt
     6781 aatcatagca ggctttgggg gaatgataaa tgaaactttt gatagaataa tgctaagctg
     6841 gtgggctcct gtacaaacag aaatggctgc aaaatctgaa gtgggtattt atgcagcatg
     6901 ttataaatta tccatactca tcactttatc tgttcaagca tttagaatgg gtgccgaacc
     6961 atttttcttt aaaaaatcta aagatgcaga tgccaaaaaa acctatgcta gggtaatgaa
     7021 tttttttgta attactgttt gctttatgtt tcttttggtt gccttatatt tagatatttg
     7081 gaagcatttt attcaaaatg ctaatatgtg gcaaggctta catattgttc ctatattatt
     7141 attggcaaac atttttttag gtatttatta taatttaagt atttggtata aactagtaaa
     7201 taaaacaaat gttggtgccc ttataaccat ttttggagta gtgataacct tggtaataaa
     7261 ctactttttt attccttatt atagctatac agcctgtgct tgggcaactt ttgcttgtta
     7321 tggtagtatg atggttttaa gttatctatg gggacaaaaa gaataccctg taccttatga
     7381 atgggtaaaa ttatgttctt atatttttta cgttgttgct ttatatttta ttcatagcat
     7441 tttaattaaa cattttacca cattttggtc tagttttact attgctagtt tattagtatt
     7501 attattttcc tttattgttt ttatcaaaga aaaatcggag cttaaaaatt tacccatttt
     7561 taataaacta attaagtaat tgttaatata gttagtaatc ctaaccatat taaataattt
     7621 cctatatttg ctttaaataa tgcaattaaa atgagagtaa ttattgttgg tggaggattt
     7681 gcaggcttaa aacttgcaag attattgaat aataaaaatg gtatagatat aactttactt
     7741 gataaatata attttcatca gtttcagcct ttattttatc aagttgcaac tgctggatta
     7801 gatgcaagca acatttcctt tcccttaaga aaagtatttc aaaaaagtaa gaatattaga
     7861 ataaggttag ctgaagtaca aaaaattgac tctaaagaaa ataaaatagt aacagatatt
     7921 ggcatttttg agtacgatta tttagtactt gcaactggtg ctaataccaa cttttttggt
     7981 aacgaacttt t
//
LOCUS       DAOQHP010000054         7989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4663_length_7989_cov_3.252710, whole genome shotgun sequence.
ACCESSION   DAOQHP010000054 DAOQHP010000000
VERSION     DAOQHP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4663_length_7989_cov_3.252710"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLY81_02935"
     CDS             complement(<1..689)
                     /locus_tag="PLY81_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29215.1"
                     /translation="MHTTKISSLSKMLLFIGALFLIISLFVPIWSIYLDAPQYPEGLS
                     LKIWANAIAGDVDIINGLNHYIGMKTLHTKDFIEFAILPYIIVGFAILFALTALMGKK
                     KLLYIIFTAFVLFGIVAMADFYRWEYDYGHNLDPNAAIKVPGMAYQPPLIGFKQLLNF
                     GAYSIPSIGGWLFISAGLLSLTAVLIETKFLKKFSKSKSTLAVLLPMIVLFSSCNNQK
                     AEPISLNKDACT"
     gene            complement(1058..3022)
                     /gene="nosZ"
                     /locus_tag="PLY81_02940"
     CDS             complement(1058..3022)
                     /gene="nosZ"
                     /locus_tag="PLY81_02940"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNF29216.1"
                     /translation="MITKKIAVGLLAVAAVYFTSCKPKGSSSAVSGDAASKTYVAPGK
                     YDEFYNFVSGGFSGQLSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNTSHGFV
                     PWDDLHHVEMSMTDAMPNGKWVFVNGNNTPRLARVDLKTFRTAEIIELPNSGGNHSSP
                     FGTENSEYIVAGTRFSVPADTDNGDVPINTYKQNFKGHISFVSVNKDNGEMNIAFQLK
                     TPGVNFDLARTGKGKSSGWFFFSTYNTEQANTLLEVNASKNDKDFIMAVNWKKAEEYI
                     KAGKGKKQSVRYAHNKFNEETHTATSEIKNEVTVLDVAEFPDIVYMIPCPKSPHGCDV
                     NPTGEYIVGSGKLAALLPVFSFEKIQKAIADKAFEAEKYNGIPVIKYEAALYGEVQKP
                     GLGPLHTEFDAEGNAYTSFFVSSEVVKWNVKDLKIIDRKPTYYSVGHLMVPGGNTKKP
                     YGKYLVAYNKITKDRYLPTGPELSQSAQLFDISGEKMQLILDFPTIGEPHYAQAVPAD
                     LIKNNQVKIFEIAKNKHPYVAKGEGETKVVREGNKVHVYITAVRSHFAPDNIEGVKMG
                     DEVYFHVTNLEQDWDVPHGFGVKGANNGELLIMPGETTTLKWVPDRVGIFPIYCTDFC
                     SALHQEMQGYVRVSPKGSNVPLTWSLGDKK"
     gene            complement(3089..3583)
                     /locus_tag="PLY81_02945"
     CDS             complement(3089..3583)
                     /locus_tag="PLY81_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF29217.1"
                     /translation="MRKKSLMMLLLVMPLFYVACSGGKTENNASTEPTEGPTTTTETA
                     DASSYDPKRGEGKYDEKNVDVTKFDAALAGQGKKVAETKCLSCHKMTDEKLVGPGWKG
                     VTQRRLPHWIMNFITNPDPMIDKDPEVQAQLELCLVRMPNQNLAENEAREILEYMRQN
                     DGAK"
     gene            3888..4547
                     /gene="purQ"
                     /locus_tag="PLY81_02950"
     CDS             3888..4547
                     /gene="purQ"
                     /locus_tag="PLY81_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNF29218.1"
                     /translation="MKFGVVIFPGSNCDRDMQYTLENDLNKEVIMLWHKDKDLSMFST
                     NDCIVLPGGFSYGDYLRCGAIARFSPMMQSVIEFANKGGKVLGVCNGFQILCESGLLP
                     GVLLQNKNQKFICNNVFIKNKNSGALKIPIAHGEGRFYADNDTLDSLEKNNQIIFSYC
                     NEVGEIEQQYNPNGTLRNIAGISNNFGNVFGMMPHPERATNYQLQNTDGIKVFEILGL
                     N"
     gene            4568..5020
                     /locus_tag="PLY81_02955"
     CDS             4568..5020
                     /locus_tag="PLY81_02955"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNF29219.1"
                     /translation="MKNLILVLAGVIFTFSVQAQKAKNPVSFSYKAVKKTDGTVDIIA
                     LATIEKGWHIYSQNTDKGGPVPTNIAFKLNPLAKKTGKVLEKGKVEKTFDKTFGVNVM
                     YYSNQVQFIQNFKIKNGIKTSIYGTVEYMVCDDEMCLPPKKENFEVKI"
     gene            5063..7009
                     /locus_tag="PLY81_02960"
     CDS             5063..7009
                     /locus_tag="PLY81_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNF29220.1"
                     /translation="MKKVILFLFLCVSSYFVFAQSENPLVWKVSYKKIDTATFTITAS
                     TTIPAAWHLYGKNETIEELTSVQFITADFENDELIGEPKFNTSPTVIKDAIFENQNLN
                     VYTGEITVTQNVKINGLIPNKLKGTVEAFIAKGDEFVPTSFVYEVSLEGGVAANTDAQ
                     KILLKSVDLNKPAAQCGHVEEVKNSSILKVFFLGILGGLIALLTPCVFPMIPVTVSFF
                     TKRSANHKEAVKNGTLYGVFIFLIYIAASIPFHLISNIQPEILNNISTNAWLNIVFFA
                     IFIFFAISFFGFFEISLPSSIAGKADSKGSLGSIGGIFFMALTLTIVSFSCTGPILGS
                     LLVGTASGGAMELTAGLAGFGIALALPFALFAVFPNWLKSLPKSGGWLDTVKKVLAFA
                     ELAFAIKFLSNADTVMHWGLLKREVFIGLWILISIGLVLYLFGFLRLPHDDRTAKISV
                     GRKIFGLIALAFTIYLIPGLTNTKHANLKLLSGFPPPKNYSIYGKESAHHKGLEANIS
                     NNYLKALQMAKEQNKPLLIDFTGWACVNCRKMEENIWTVPEVKQFITDHFILLSLYVD
                     DKEKLPVADRFTYKTKSGFEKDIVTVGDKWSTFQTENFGQTTQPLYVILDTEEKLLNN
                     PIGYTPSYNEYLKWLQCGKNAFQK"
     gene            7072..7458
                     /locus_tag="PLY81_02965"
     CDS             7072..7458
                     /locus_tag="PLY81_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF29221.1"
                     /translation="MMKLNSVGIGTRILNFLVDIILIFLLAYVAFKIHKWYVFYYKIT
                     YINFGWFFFGTMFFYCLLFETFFAKTPGKWFTQTKVVNLSGNKPSFFAIVLRSFIRLT
                     IIDVFFIPFIDKTLHDYVSKTVVVET"
BASE COUNT         2779 a         1196 c         1191 g         2823 t
ORIGIN      
        1 gtgcatgcat ctttatttaa agaaattggt tcggcttttt ggttattgca agaagaaaat
       61 aaaactatca ttggtaacaa aacagccaat gtagatttac ttttgctaaa cttttttaaa
      121 aacttagttt ctattaaaac tgcagttaac gataataaac cagcagaaat aaaaagccaa
      181 cctccaatgc ttggtattga atatgcacca aagtttagta attgtttaaa ccctattaat
      241 ggtggttggt aagccattcc tggaacttta attgcagcat tggggtctaa attgtgccca
      301 taatcgtact cccatctata aaaatctgcc atggcaacaa taccaaataa aacaaaagct
      361 gtaaaaatta tatacaataa tttcttttta cccattaaag ctgttaatgc aaataaaata
      421 gcaaacccaa caattatata aggtaaaatt gcaaattcaa taaaatcttt agtatgtaaa
      481 gttttcatgc caatataatg gtttaaccca ttaataatat caacatcgcc agcaatagca
      541 ttagcccaaa tttttaaact caaaccttct ggatattgag gagcatctaa ataaatactc
      601 cagataggaa caaataatga aataataaga aataaggcac caataaaaag taacatttta
      661 gaaagggatg aaatttttgt agtatgcatt atataaaatt tatatggttt aagggtaaat
      721 tttgagttgc aaaattatca ttatatataa taatacccta tgacctcaat catgaaactg
      781 cttaaagaaa actagaatta taatttatac tcttttgaga aaaggcttta aaaaaaatat
      841 taaaaagtta gagttattaa acctaaagca ataggtaaaa taaaattaaa ttacatttat
      901 tggggcgttg ttattaacag ctagaaatgg aacaaaagct attgaaaaaa atatttaaac
      961 aattttcttt attttactgc ggaataactt tataaaaact aaaatgctgc ctaaaaatag
     1021 taggcagcat tttaatattt aactaataaa aaaagactta ttttttatca cctaaactcc
     1081 aagttaaagg tacattacta cctttaggag aaactcttac ataaccttgc atttcttggt
     1141 gtaatgcaga gcaaaaatct gtacaataaa ttgggaaaat acctactcta tctggtaccc
     1201 attttaatgt ggtagtttca ccaggcataa taagtaactc gccattatta gcacctttta
     1261 caccaaagcc atgtggtacg tcccaatctt gttctaagtt ggttacatga aagtaaactt
     1321 catctcccat tttaacacct tctatattat ctggtgcaaa gtgagaacga acagcagtaa
     1381 tgtaaacatg tactttattt ccttctctta caaccttagt ttcaccttct cctttagcta
     1441 cataagggtg tttattttta gcaatctcaa atatttttac ttggttattt ttaattaaat
     1501 cggcaggtac cgcttgtgca tagtgtggtt cgccaatggt tgggaagtct aaaattaatt
     1561 gcattttttc tccactaata tcaaataact gagcactttg agaaagctct ggaccagtag
     1621 gtaaataacg atctttagta attttgttat aagctactaa atatttacca taaggttttt
     1681 tagtattacc acctggaacc attaagtgac caacagaata gtaagttggc ttacgatcaa
     1741 taatttttaa atcttttaca ttccatttta ctacttcaga agaaacgaag aaggaagtat
     1801 atgcattacc ttcagcatca aactctgtat gcaaagggcc taatcctggt ttttgtactt
     1861 cgccatataa agcagcttcg tatttaataa caggaatacc attgtatttt tcagcctcaa
     1921 atgctttatc tgcaatagct ttttgaattt tttcgaagct aaatacaggt aataaagctg
     1981 ctaatttacc actacctaca atgtattcac ctgttgggtt tacatcgcaa ccatgtggag
     2041 atttagggca aggaatcatg taaacgatat ctggaaattc agcaacatct aaaactgtta
     2101 cttcattttt aatttctgaa gtggcagtat gtgtttcttc attaaattta ttatgggcat
     2161 atcttacaga ttgtttttta ccttttcctg ctttaatata ctcttctgct tttttccagt
     2221 ttacagccat gataaagtct ttatcatttt tagaagcatt tacttctaac aatgtgttag
     2281 cttgctctgt attgtaagta ctgaagaaga accaaccact agatttacct ttacctgtac
     2341 gtgcaaggtc aaagtttaca cctggtgttt ttaattggaa agcaatattc atttcgccat
     2401 tatctttatt aacacttaca aagctaatgt gacctttaaa attttgcttg taagtattaa
     2461 ttggcacatc accgttatct gtatctgcag gaacactaaa acgtgtacca gcaacaatat
     2521 attcagaatt ttcagtacca aatggcgaac tgtgattacc accactatta ggtaattcta
     2581 taatttcggc agtacggaaa gtttttaaat ctactcttgc taaacgaggg gtgttattac
     2641 catttacaaa tacccattta ccattaggca ttgcatctgt catagacatt tcaacgtggt
     2701 gtaaatcatc ccaaggtaca aaaccatgag aagtatttaa cattggctta gtttcttcgc
     2761 tatatcccca gcctttttca ggatcaacag agaatacagg aattacacgt aataaacgac
     2821 cacttggtaa gccataaaca cttaattggc cactaaagcc accagatacg aagttataaa
     2881 attcatcgta tttacctggt gctacataag ttttagaagc agcatcgcca cttactgcac
     2941 tactagagcc ttttggttta cacgaagtaa aataaacagc agctacagca agtaagccaa
     3001 cagctatttt ttttgttatc ataatttatt ttttaaggat ttaagaattt gaaaattggt
     3061 tgtataaaat gagctatcta aatattattt atttagcacc atcattttga cgcatatact
     3121 ctaatatttc tctagcttca ttttcagcta aattttgatt aggcatacgt actaaacaca
     3181 attcaagttg tgcttgtact tctgggtctt tatcaatcat tgggtctggg ttggtaataa
     3241 agttcataat ccaatgtggt aatcttcttt gtgttacacc tttccaacct gggccaacta
     3301 atttttcatc ggtcatttta tgacaagata aacatttagt ttctgccact tttttacctt
     3361 gtcctgctaa agcagcatca aacttggtaa catctacatt cttttcatca tacttccctt
     3421 caccacgttt aggatcatag ctagaagcat cagcagtttc tgtagttgta gtagggcctt
     3481 cagtaggctc tgtagatgca ttattttcgg ttttacctcc actacatgca acatagaata
     3541 aaggcattac taaaagtagc atcattaacg atttttttct cataattttc aatgatttaa
     3601 aattttatgc aaaagtaaat tgtgtatgtt attttaaggt atgacttaag tcatatttaa
     3661 gaaaaatatt tatttatttt ttaaactatt tttccatata acaccactta aaaacagttg
     3721 tgtgaaatta ttaaaaacaa aagtataaaa atatattaag cgttgcttat tttcttttgt
     3781 tatagctgtg tatttattaa agaaattaaa aagtgtataa ttgttttcgt aaatcaaata
     3841 tttaatacag ttaatgcaag tacctttgcc accaatttat acaaattatg aaatttggag
     3901 ttgttatttt tccaggctct aactgcgata gagatatgca gtatacttta gaaaacgatt
     3961 taaacaaaga agtaattatg ctttggcata aagataaaga tttaagtatg ttttctacta
     4021 acgattgtat tgttttacca ggtggctttt cttatggcga ttatttacgc tgtggagcta
     4081 ttgcaaggtt tagcccaatg atgcaaagtg ttattgaatt tgccaataaa ggtggaaagg
     4141 ttttaggagt atgtaatggc tttcaaattt tatgcgaaag tggtttactg ccaggcgttt
     4201 tattacaaaa taaaaatcaa aaatttattt gcaataatgt atttatcaaa aataaaaatt
     4261 caggagcttt aaaaatacct attgcacatg gcgagggaag attttatgct gataatgaca
     4321 ctttagatag tttagaaaaa aacaaccaaa ttatttttag ttattgtaat gaagttggtg
     4381 aaatagaaca acaatataat cctaacggaa ctttaagaaa tattgctggt ataagtaata
     4441 actttggcaa tgtttttgga atgatgcctc atcctgaaag agctacaaac taccaactac
     4501 aaaacaccga tggaataaaa gtatttgaaa ttttaggact taattaatta taaatttaaa
     4561 cccaaacatg aagaatttaa ttttagtatt agcaggtgta atttttacat tttctgtaca
     4621 ggcacaaaaa gcaaaaaatc ctgtaagttt ttcttacaaa gcagtgaaaa aaacagatgg
     4681 aactgtggat attattgcat tggctacaat tgaaaaaggc tggcatatat attctcaaaa
     4741 tacagataaa ggcggcccag tacctaccaa tattgctttt aagttaaacc ctcttgcaaa
     4801 aaaaacaggt aaggtattag aaaaaggtaa agtagaaaaa acatttgata aaacctttgg
     4861 agttaatgta atgtattata gcaatcaggt acagtttatt caaaatttta aaattaaaaa
     4921 cggaattaaa acatctattt atggtactgt agagtatatg gtttgcgatg atgaaatgtg
     4981 tttgcctcct aaaaaagaaa actttgaagt aaaaatttaa tgattacttc agttttttac
     5041 taccttttta aaacaccagt acatgaagaa agttattctg tttttgtttt tatgtgttag
     5101 cagttatttt gtatttgcac aaagtgaaaa tcctttagtt tggaaagtaa gttataaaaa
     5161 aattgatacc gctactttta ccattacagc aagtacaact atacctgcag catggcattt
     5221 atatggcaaa aatgaaacca ttgaagaact aaccagtgta caatttatta ctgctgattt
     5281 tgaaaatgat gaattaatag gagaacctaa atttaatact tcgcccacag ttattaaaga
     5341 tgccattttt gaaaaccaaa acttaaacgt ttatacagga gaaattacag ttactcaaaa
     5401 tgtaaaaata aatggcctta tacctaataa attaaaaggt actgtggagg catttattgc
     5461 taaaggagat gaatttgtac ctacctcttt tgtttatgaa gtaagcctag aaggtggtgt
     5521 agctgctaat acagatgcac aaaaaatatt attaaaatct gttgatttga ataaaccagc
     5581 agctcaatgt ggccatgtgg aagaagtaaa aaattctagt attttaaaag tatttttttt
     5641 aggtattttg ggtggtttaa ttgccttact tacaccatgc gtttttccta tgatacctgt
     5701 tacagtatcc ttcttcacca aaagatctgc aaaccataaa gaggctgtaa aaaatggtac
     5761 tttatatggt gtttttattt ttttaatcta tatagctgca agtattcctt ttcatttaat
     5821 tagtaatata cagccagaaa tattaaataa tatttctacc aatgcatggt tgaatattgt
     5881 attctttgcc atctttatat tttttgccat ttcatttttt ggttttttcg aaatatcctt
     5941 accatctagt attgcaggta aagcagatag taaaggaagt ttaggcagta taggtggaat
     6001 tttctttatg gcattaactt taactattgt ttcattttct tgcactggcc caattttagg
     6061 ttcattattg gtaggtacag caagtggtgg ggcaatggag cttacagcag gtttagcagg
     6121 ttttggtatt gcattggcat tgccttttgc gttatttgca gtttttccca attggctaaa
     6181 atctcttcca aagagtggtg gttggttaga tactgtaaaa aaagttttag cttttgccga
     6241 gttagctttt gcaattaaat ttttatctaa tgcagatact gttatgcatt ggggcttatt
     6301 aaaacgagaa gtttttattg gcttgtggat attgataagt attggattag ttttatattt
     6361 atttggattc ttaagattac cacacgacga cagaacagct aaaataagtg tgggtcgtaa
     6421 aatatttggt ttaatagctt tggcatttac catttattta atacctggtt taactaacac
     6481 caagcatgcc aaccttaaac ttttaagtgg ctttcctcct ccaaaaaatt atagtattta
     6541 tggcaaagaa agtgcacacc ataaaggctt agaagctaac atttctaaca actatttaaa
     6601 ggcactacaa atggctaaag aacaaaataa gcctttgcta attgatttta ctggttgggc
     6661 ttgtgtgaat tgtagaaaaa tggaagaaaa tatttggaca gttccagaag ttaaacaatt
     6721 tattacagac cattttattt tactttcttt atatgttgat gataaagaaa aactacctgt
     6781 tgccgaccga tttacttata aaaccaaaag tggttttgag aaggatattg ttacagttgg
     6841 agataaatgg tcaacctttc aaaccgaaaa ttttgggcaa acaacccaac ctttatatgt
     6901 tatattagat actgaggaaa aattgttgaa taatcctatt ggctatacac caagctataa
     6961 tgaatattta aaatggttgc aatgcggtaa aaatgctttt caaaaataaa tatttattac
     7021 tactctatgt aactaaatta taatttaaaa aatgttttta atttatacca aatgatgaaa
     7081 ttgaatagtg ttggcattgg tactcgtatc ctaaactttt tggtagatat tatccttata
     7141 tttttacttg cttatgtagc ttttaaaatt cataaatggt atgtgtttta ctacaaaata
     7201 acctatataa attttggatg gtttttcttt ggtacaatgt tcttctactg tttactattc
     7261 gaaacatttt ttgcaaaaac accaggcaaa tggtttactc aaactaaagt agttaatcta
     7321 tctggcaata agccttcttt ttttgccata gttttaagaa gttttataag gctaactatt
     7381 attgatgtat tttttattcc ttttattgat aaaacccttc acgattatgt aagtaaaaca
     7441 gttgttgtag aaacttagtc ccatttcgct tcttttataa aaaatacact tatacttaat
     7501 aacctacatt ctctttaaat atattttaag aaaactaatt cgtattcact tctctaactt
     7561 ttacatattg attctctaaa gtagtattag ccaacattaa agaaagacat ttttttaaaa
     7621 aacactacaa tcatcaaata attttattaa ttactatatt tatttgtata ttattgaata
     7681 tcaatacaaa ataatattaa taacaatagt caatggttaa ttcactaaaa aattaatacc
     7741 cataaaaggt aaaaatataa actaactgga taatttatct aaattcaata gtcaaaataa
     7801 ttttaaagat aattttatct ttattttacg ttacaattgc aaaattcttt taatctggtt
     7861 ttataaatat tttttgttat taataataaa agcaaaaaat actagatggt tgctgtattt
     7921 taaaaattaa attctgagtc aaaattttaa acttatttat tatgaaaaag tttttactac
     7981 tttttacct
//
LOCUS       DAOQHP010000055         7915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4732_length_7915_cov_3.902417, whole genome shotgun sequence.
ACCESSION   DAOQHP010000055 DAOQHP010000000
VERSION     DAOQHP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_7915_cov_3.902417"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLY81_02970"
     CDS             complement(<1..214)
                     /locus_tag="PLY81_02970"
                     /inference="COORDINATES: protein motif:HMM:NF023724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3630 family protein"
                     /protein_id="HNF29222.1"
                     /translation="MSYQLTNFSGAVEAVIDDNCGISKFHNIVHIMLQELKVKFLAKE
                     DDSDTVDWHFKYKGRPLTLHYNIYNGV"
     gene            complement(292..534)
                     /locus_tag="PLY81_02975"
     CDS             complement(292..534)
                     /locus_tag="PLY81_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29223.1"
                     /translation="MKQFKAVIIILILAVVLSSCNFFRNIFGGGDKYGCPTSVNNIGA
                     EKLSGDYKPKNREEAKAIKRAAKANKKAAKRNPYMY"
     gene            complement(669..1202)
                     /locus_tag="PLY81_02980"
     CDS             complement(669..1202)
                     /locus_tag="PLY81_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNF29224.1"
                     /translation="MAYILTVLNKTPNMGNNCFIAPNATIAGDVVMGNECSIWFNAVV
                     RGDVNYIKMGNKVNVQDGAVIHCTYQKNATNIGNNVSIGHNAIVHGCTIHDNVLVGMG
                     AIVMDRCIVHSNSIIAAGAVVLEGTVIEEGSIYAGVPAKKVKEISQDLIKGEIDRIAN
                     NYIQYASWFEKYNDAAK"
     gene            complement(1242..2516)
                     /locus_tag="PLY81_02985"
     CDS             complement(1242..2516)
                     /locus_tag="PLY81_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNF29225.1"
                     /translation="MAEIFIIIGLIIINGLFSMAEIALVSARKARLEAQANKGNEEAE
                     RALKLANKPDSFLSTVQIGITLIGILTGFFSGENIKNDVVIWLQKFEILQPHSDKLAT
                     ALVVVVITYFTLVLGELLPKRIGLSNPEVIAKMVATPMMFISKIAFPFIWLLNKSTNT
                     LSKLFNILPNNSQVTEDEIKAIINEGTEQGTIEEAEQEIIERVFRLGDRNITSLMTHR
                     SDIIWLDVHKTIGELKSKFEEGIFSVYPVCDGSIDNIKGSVTIKDLFISDSNTNISTL
                     VKQPLYVPENNTAYQVLEKFKETKIHHCYIVNEYGSLEGMITLNDILEAIVGDIPEIT
                     EDNYEIIEREDGSLLADAQIPFIDFLHKVGKEDWINDEEDFDFDTLAGFILHHTQEIP
                     TTGERLQWRDFEFEIIDMDKHRIDKILISYKK"
     gene            complement(2599..4101)
                     /locus_tag="PLY81_02990"
     CDS             complement(2599..4101)
                     /locus_tag="PLY81_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNF29226.1"
                     /translation="MQFSKDIFISYAHIDDESLIASQRGWISEFHRALEIRLAQLLGR
                     RPNIWRDMSLQGNHIFDQQIVSQFANVAIMISILTPRYVKSDWCIREVNEFYNACEQN
                     IGFNIQNIARIFKVIKTPIKIESHPEKIQNVLGYEFFSTDSNTGRVKEFGQVFGQQSE
                     LAYWEKLDDLANDICSFLETLESRTPTSETTNSTNDNFTKNRKKKIYLAEGSYDTLEF
                     RDSIKRELLDLDYTLLPDKQLPLVAPVLNNEVYSFLQNADLSIHLIGSGYGVVPEGTQ
                     KSIVEIQNEIASTISATKSLPRIIWVPENNVANDERQQNFLKKLQAGNEGIAGADLIV
                     SSLEDLKSIITDKLEKIKEEKNKDITESISTTNSTNKTKVVYLICDTNDLEIIQPLED
                     VLYNCGYEVLLPIFDGEETQIREDHIENLKICDTAIVFFGNGNELWLRSKMRDFIKIN
                     GYGRPQPLTSKIVYLAAPDNATKQRFRSVEAEVINGIHNVPEEKLISLLK"
     gene            complement(4113..6071)
                     /locus_tag="PLY81_02995"
     CDS             complement(4113..6071)
                     /locus_tag="PLY81_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF29227.1"
                     /translation="MNAPNPKFNPILFPLIVVLVFTFSIIGHYSYLAHEGHAFNLFGV
                     IYATICFFVMHHVDPVPHNTYILVAQYLAALLICTSIFSLVFAKVKKAYLVYRIGITY
                     RNHIVIFRLNNIGKTLAIELLQKGYKVIVVESDEQNPFMEAIKLNGGIVFTEKPTDYK
                     TLKMAAVSYSRVCILTSNRDEINIEIAGTLASYLLKYSGRKKGDPHKIMVHINNTSNE
                     NLLKDYFDIQNEDDHYDLETFNVYESAAKKIYDTYSPYKYIDVSDKESENAIAIIGFN
                     DVAESFIVENMILSHYPDKERIRIYLVDDHADEKFNYISYKYPFFQEFIDLVPIKLIN
                     GTFFGNFTWSKVHIENLSKIKVVYVFGQKDAEIINISTNFRQFLYTQTLSISQVPIIA
                     CLPEDTGVLDLLNSNRESGETLSQVFSNKLNINTYRLITNTCTAEGLIEESETTQRLS
                     KLINFFYAIKYEFAAILENKWNISNAVSITNLLEEELLGLPDKYEKLHEREIEKHLLT
                     LLSLKTNIPTSDLYDNFSIEKHWQHLSYRKKDSNKYVARQIATKIQMLQYIGCTPINK
                     QNITTFFPRLAPIEHIRWSAEKMVFNFKYGPYPTDKNEKYLLKEVLKIHDQLVSYEKL
                     NEFDQEKDLNLFLMIPVLSIIKTNNGTK"
     gene            6578..7051
                     /locus_tag="PLY81_03000"
     CDS             6578..7051
                     /locus_tag="PLY81_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNF29228.1"
                     /translation="MQIKLNFDKLDLQIIHSLINNADISYADLGKKLFVSAGTIHVRI
                     KKLEEHGIVKGKLLAVDYKNLGYDVIAFVGIYLEKSSLYDEVAKSLKKIPEIVRLNYT
                     TGNYSMFIEIVCKDIQQLRKVLHDKLQNIKGIERTETLISLEESFRRNILFIDEN"
     gene            complement(7102..>7915)
                     /locus_tag="PLY81_03005"
     CDS             complement(7102..>7915)
                     /locus_tag="PLY81_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF29229.1"
                     /translation="RVRNTPLCESAIIGTALGLSLEGNKSMVEMQFADFVSVGFNQIV
                     NNLAKIHYRWGQNADVIVRMPCGGGVGAGPFHSQTNEAWFTKVAGLKVVYPATPYDAK
                     GLLIAAINDPNPVLFFEHKALYRSIQGHVPKRYYEIEIGKANTVKQGNDITVITYGAG
                     VHWVLEFLEKNKNVSCELLDLRTLQPLDYEAIKNAVIKTGKALIVHEDSLTGGFGAEI
                     SAWINEHCFDKLDAPVMRCASLDTPIPFNTDLEKNYLAKSRLSEYFNKLLNY"
BASE COUNT         2593 a         1276 c         1091 g         2955 t
ORIGIN      
        1 aaacgccatt gtaaatatta taatgtaaag taagcggacg acctttgtat ttaaaatgcc
       61 aatctactgt atcgctatca tcttcttttg ctaagaactt tacttttagc tcttgtagca
      121 taatgtgtac aatgttatga aatttactaa ttccacaatt gtcgtcaata acagcctcca
      181 cggctcctga aaagttggtt aattggtagc tcatagaaat aaagttttat ggatttaaaa
      241 attggatata aagatttttt aactattttc tattaaccaa gaataccctt tttaatacat
      301 gtatgggttt ctttttgctg cctttttatt ggctttagct gctcttttta ttgcttttgc
      361 ttcctctcta ttctttggtt tataatcgcc tgataacttt tctgctccta tattatttac
      421 acttgttggg cagccatatt tatctcctcc tccaaaaata tttctaaaaa aattacaaga
      481 acttagaaca acagccaaaa taagtataat tataactgct ttaaattgtt tcatgaattt
      541 attttttgaa ctgcaaagtt atcatatacc aataagttca gtagtaattt ttatcaaaaa
      601 tctataaaaa gctttaaaac tgggttaaaa cttttgtttg tacattatat aacggcataa
      661 tataaaattt attttgctgc atcattatat ttttcaaacc aagaggcgta ttgaatataa
      721 ttgttggcaa tacggtctat ttcgccttta attaaatctt gagaaatttc tttaactttt
      781 tttgctggca cacctgcata aatacttcct tcttcaataa cagtgccttc taaaactact
      841 gcacctgcag caataatact gttactatgt acaatgcatc tatccattac tatggctccc
      901 attcctacta aaacattatc gtgtattgta cagccatgca caattgcatt atggccaatg
      961 gaaacattgt ttccaatatt ggtagcattt ttttgataag tacaatgtat tacagctcca
     1021 tcttgcacat ttactttgtt gcccattttt atataattca catcgcctct tacaactgca
     1081 ttaaaccata tactacactc atttcccatt actacatctc ctgcaatggt tgcattgggt
     1141 gctataaaac aattattgcc catgttgggc gttttattta gtactgtaag aatatatgcc
     1201 atacaacagg tttatatttt tagaataaag ttactctttg cttatttttt gtaagagatt
     1261 aatattttat caattctatg tttatccata tctataattt caaattcaaa atcacgccat
     1321 tgcaatctct ctcctgtagt aggtatttct tgtgtatgat gaagtataaa gcctgcaagt
     1381 gtatcaaaat caaaatcttc ctcatcattt atccagtctt cttttcctac tttatgtaaa
     1441 aaatctataa agggtatttg agcatctgcc aataagcttc catcttctct ttcaattatt
     1501 tcataattgt cttctgttat ttcaggaata tctcctacaa ttgcttctaa aatatcattt
     1561 aatgttatca tgccctctaa acttccatat tcgttaacta tataacaatg atgaattttt
     1621 gtttctttaa atttttctaa tacctggtaa gcagtattat tttctggcac atacaaaggt
     1681 tgttttacta aggttgaaat attagtgttg ctatcgctaa taaacaaatc tttaattgtt
     1741 acactacctt ttatattatc tatacttcca tcgcaaacag gataaacgga aaaaatacct
     1801 tcttcaaatt tactttttag ttcgccaata gttttatgta catctaacca aataatatcg
     1861 cttctatgcg tcattaacga ggttatattt ctatcgccta atctaaaaac tctttcaata
     1921 atttcttgtt ctgcttcttc aattgtacct tgctctgtac cctcattaat aattgctttt
     1981 atttcatctt ctgttacttg agagttgtta ggtaaaatat taaaaagttt agataaagtg
     2041 ttggtagatt tatttagaag ccaaataaaa ggaaatgcaa ttttagatat aaacatcatt
     2101 ggtgtagcaa ccatttttgc tataacttct gggttactta acccaattct ttttggtaac
     2161 aactcaccta aaactaaagt aaaataagta ataaccacta ctactaaagc agttgccaac
     2221 ttatcgctat gtggttgtaa tatctcaaat ttttgaagcc aaataaccac atcatttttt
     2281 atgttttcac cagaaaaaaa accagttaaa ataccaatta gtgtaatgcc aatttgtact
     2341 gttgatagaa aagaatcggg tttgttggct aattttaaag ctctttctgc ttcttcattg
     2401 cctttattgg cttgtgcctc aagccttgct ttacgtgccg aaactaatgc aatttcggcc
     2461 attgagaata gcccattaat aataattaac ccaataataa taaaaatttc agccatgata
     2521 ataataacgc aagatatatg tttttaattg attaagtaaa aaggctggct gtagtatatg
     2581 tatttgataa aaaattaatt attttaatag actaattaat ttttcttctg gtacgttgtg
     2641 aatgccatta ataacttcgg cttcaaccga tctaaatctt tgtttagtgg cattatctgg
     2701 tgctgccaag taaacaattt tagaggtaag tggttgaggc cttccataac catttatttt
     2761 aataaaatct ctcattttac ttcttaacca tagctcatta ccattaccaa aaaaaactat
     2821 tgcagtatcg caaatcttta aattttctat atggtcttct ctaatttgtg tttcttctcc
     2881 atcaaatatg ggtagcaaaa cttcgtagcc acagttgtac aatacatctt ctaatggttg
     2941 tattatctct aaatcgttcg tgtcgcaaat taagtacact acttttgttt tgttggtact
     3001 attggttgtt gagatacttt cagtaatatc tttatttttt tcttctttaa ttttttcaag
     3061 cttgtcggta ataatacttt ttaaatcttc taaactactt acaattaaat ctgcaccagc
     3121 aataccttca ttacctgctt gtaatttttt taagaaattt tgctgccttt catcgtttgc
     3181 aacattgttt tcaggtaccc aaattatacg aggtaatgat ttagttgcac ttattgtgga
     3241 ggctatttcg ttttgtattt caacaattga tttttgtgta ccttctggca caactccata
     3301 cccagagcct attaaatgaa tgcttaaatc agcattttgt aaaaaactgt aaacttcatt
     3361 atttaataca ggagccacta atggtaattg tttatctggc aatagagtgt aatctaaatc
     3421 taataattct cgtttaatac tgtctctaaa ttctaaagta tcgtagctac cttcggctaa
     3481 ataaattttc ttttttctat tttttgtaaa attgtcattt gttgaattgg ttgtttcgga
     3541 agtgggagtt ctgctttcga gggtttctaa aaaagaacat atatcgttag ctaaatcatc
     3601 taatttttcc caatatgcca actcagattg ttgaccaaat acttgtccaa attcttttac
     3661 tctaccagta ttagaatcag tactaaaaaa ttcatagccc aatacatttt gaattttttc
     3721 tgggtgcgat tctattttaa ttggtgtttt aataacttta aaaattctgg caatattttg
     3781 aatattaaaa ccaatatttt gctcacaagc attgtaaaat tcatttactt ctctaataca
     3841 ccaatcgctt tttacatatc ttggtgtaag aatagaaatc ataattgcca cattggcaaa
     3901 ttggctaaca atttgttggt caaaaatatg attgccttgt aatgacatgt ctcgccaaat
     3961 attaggtctt ctgcctaaaa gttgtgcaag cctaatttct aatgctctgt gaaattcact
     4021 tatccagcca cgttgtgatg caattaaaga ctcatcatca atatgagcat aactaataaa
     4081 tatgtcttta gaaaattgca tgcttttaag gcttatttag ttccattgtt tgttttaatt
     4141 atgcttaata caggaatcat taaaaataaa ttcaaatcct tttcctggtc gaactcattt
     4201 agtttttcat aagaaactaa ttggtcgtga atttttaaaa cttcttttaa taaatatttt
     4261 tcgtttttat ctgttggata tggcccatac ttaaaattaa aaaccatttt ttctgcactc
     4321 catctaatat gctctatagg ggcaaggcgt ggaaaaaatg tagtaatatt ttgtttgttt
     4381 attggtgtac aaccaatgta ttgtaacatt tgaattttag ttgctatttg tcttgcaaca
     4441 tatttattcg aatctttttt tctgtaagat aaatgttgcc aatgtttttc aatagaaaaa
     4501 ttatcatata aatcggaagt tgggatattt gtttttaaag aaagtagagt taataaatgt
     4561 ttttctattt ctctttcgtg tagtttttca tatttatctg gtaagccaag caattcttct
     4621 tctaataaat tggtaataga tacagcattg cttatattcc atttattttc taaaatagct
     4681 gcaaattcat atttaattgc ataaaaaaag ttgatgagtt tagaaagcct ttgcgtagtt
     4741 tcagattctt caattaatcc ctcggcggta caggtattag ttattaaacg ataagtgtta
     4801 atattaagtt tattgctgaa aacttggctc aatgtttcgc cagattctct gttggaattt
     4861 aataaatcca acacacccgt atcttctggc aaacatgcaa taataggtac ttgagaaatg
     4921 gataaggttt gagtatataa aaattggcgg aagttggtag agatatttat tatttcagca
     4981 tctttttgcc caaaaacata aaccactttt attttactta aattttcaat atgcacttta
     5041 gaccaagtga agttgccaaa aaaagtacca ttaattaatt taataggcac taagtctata
     5101 aattcttgaa agaaaggata tttataactg atataattaa atttttcatc ggcatgatca
     5161 tctactaaat aaattctaat tctttcttta tccggataat ggctaagaat catgttttca
     5221 acaataaaag attcggccac atcattaaat ccaattattg caattgcatt ttccgattct
     5281 ttatctgaca catcaatgta tttgtatgga gaataggtgt cgtaaatttt ttttgctgcc
     5341 gattcatata cattaaaagt ttctaaatca taatggtcat cttcgttttg aatatcaaaa
     5401 taatctttta ataaattttc attggaagtg ttgttaatat gaaccattat tttgtgtggg
     5461 tctccctttt ttctgccact atattttagt aggtacgatg ctaaagtgcc tgctatttct
     5521 atatttattt catctctgtt gctggttaat atgcatactc ttgaatagct tacagcagcc
     5581 atttttaatg ttttatagtc tgttggtttt tcggtaaaaa caattccacc atttagttta
     5641 atggcttcca taaaaggatt ttgttcatca ctttccacta caattacttt ataacctttt
     5701 tgcaaaagct ctattgctaa tgttttacca atattattta acctaaaaat tactatatga
     5761 tttctgtaag taataccaat acgataaact aaatatgctt ttttaacttt tgcaaatact
     5821 aatgaaaaaa tgcttgtaca aataagtagt gctgctaaat attgagcaac taatatataa
     5881 gtgttgtgag gcacagggtc aacatggtgc attacaaaaa aacaaatagt agcataaatt
     5941 actccaaata agttaaaagc atgtccctca tgagccaagt aagaataatg accaatgata
     6001 gaaaatgtaa aaactaatac cactataaga ggaaataaaa tgggattaaa ctttggatta
     6061 ggagcattca tattttatgg gtaataatta aaccttgaaa aattttggta ctaacaaata
     6121 taatggttta agaaatatag gatattttat aactgattta tgaagaatag attactaaaa
     6181 tacctctaat aaaaaagcct ttacaagata atgctgtaaa ggcttttaaa tgtaagttgc
     6241 tgtaggggaa gggttcgaac cttcaagagg tagttagcca gagagcaaaa aaatctcaat
     6301 tgccattgag aaaactggtg gtcgacccca gtcgctattc gtgcacaaac agcggcatta
     6361 cgctccgttt attccattat cctcaccccc gagacaagag ggcatgtctg ccaaaaattt
     6421 catcacccca cagtataaag aacttttttg tttgatagtg caagtatata aaattttatt
     6481 taataaattg tacaatattc aaaaaaattt ataaaattta tattatgtat aaaatattat
     6541 atctttaatt aaatattctt aaaaaagcac tataaaaatg cagataaaat taaattttga
     6601 caaattagac cttcaaataa tacattccct tatcaataat gcagatattt cttatgcgga
     6661 tttaggaaaa aaactttttg ttagtgcagg aaccatacat gtaagaataa aaaaattaga
     6721 ggaacatggt attgtaaaag gtaaactttt agctgttgat tataaaaatt tagggtatga
     6781 tgtaattgcc tttgttggta tatatttaga gaaaagttct ttatatgatg aagtagcaaa
     6841 atcattaaaa aaaattccag aaattgtaag attaaattat acaacaggta attatagtat
     6901 gtttatagag attgtttgta aagatattca gcaattaaga aaagtcttac atgataaatt
     6961 acaaaacatt aaaggcattg aaagaacaga aactctaata tcattagaag aaagttttag
     7021 aagaaatatt ttatttattg atgaaaatta aaaaattagt ataaatattt tttattgaaa
     7081 agtggattat cctttattgt attaataatt aagtagctta ttaaaatatt cggataacct
     7141 acttttagct aaatagtttt tctctaaatc tgtattaaaa ggtattggtg tatctaagct
     7201 tgcacaacgc ataacaggag catctaactt atcaaaacaa tgttcattta tccaggcact
     7261 tatttcggca ccaaagccac ctgttaaact atcttcgtgt acaattaaag ccttacctgt
     7321 ttttataaca gcatttttta tggcttcata atctaatggc tgcaaggttc ttaaatctaa
     7381 aagttcgcaa cttacatttt tattcttttc taaaaattcc aatacccaat gtacgcctgc
     7441 accataggta ataacagtta tatcattacc ttgtttaacg gtattggctt tgccaatttc
     7501 tatttcgtaa tatcgtttag gcacatgtcc ttgaatactt ctgtacaaag ctttatgttc
     7561 aaaaaatagt acagggttgg ggtcgttaat tgctgcaatt agtaatcctt ttgcatcgta
     7621 tggtgtagca ggataaacta cttttagccc tgcaactttt gtaaaccaag cttcatttgt
     7681 ttggctatga aatggcccag cacctacacc accaccacag ggcattctta caataacatc
     7741 tgcattttgc ccccaacgat aatgtatttt agctaaatta tttacaattt gattaaagcc
     7801 tacacttaca aaatcggcaa attgcatttc taccatactc ttatttccct ctaagcttaa
     7861 acccaatgca gtaccaatta tagcactttc gcaaagtggt gtattgcgta ctctt
//
LOCUS       DAOQHP010000056         7901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4747_length_7901_cov_3.599669, whole genome shotgun sequence.
ACCESSION   DAOQHP010000056 DAOQHP010000000
VERSION     DAOQHP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4747_length_7901_cov_3.599669"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLY81_03010"
     CDS             complement(<1..412)
                     /locus_tag="PLY81_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF29230.1"
                     /translation="MSKYITMLEPWRIGKVIKIEQETANTRRYWIEIEELSNFEFTAG
                     QFVTLDLPIHEQKNKRWRSYSIASSPDGTNVIELVIVLLEGGLGTTYIFNNIEVGSTF
                     TLRGPQGKFVLPENIDKDIFLICTGTGIAPFRSMV"
     gene            527..1021
                     /locus_tag="PLY81_03015"
     CDS             527..1021
                     /locus_tag="PLY81_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF29231.1"
                     /translation="MKNLFIIRHAKSSTNFLNNNDFERPLNEQGIKDAALMAKKLLSK
                     GIIIDGIVTSTAIRAATTANYFATAFAINKNNFWNEPTLYNANKKTFYTIINNWQFTL
                     NNIAIFSHNPGITHFANSVTNSKIDNMPPCGVFAIKINCNKWQSFETSEKKFWFFDCP
                     EKIW"
     gene            complement(1029..1856)
                     /gene="folP"
                     /locus_tag="PLY81_03020"
     CDS             complement(1029..1856)
                     /gene="folP"
                     /locus_tag="PLY81_03020"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNF29232.1"
                     /translation="MFTLNCKGELLTITKPIVMGIINTSPNSFYTNSQQNSIDSALFK
                     VEQMVQEGAIIIDIGGQSTKPNSLPITVEEELRRTVNVIEAIKKKFSNLIISIDTYNA
                     TVASKAVEAGASIVNDISGGTFDKNMMATVANMNVPYVCMHIKGTPQTMQQNPIYDNV
                     TQEVLSFFIDKINQAKQAGIKDVIIDVGFGFGKTIQHNFQLLKELSVFSILEKPILIG
                     VSRKSTIYKTLGVTPEQALNGTTALHTICLLNGANILRVHDVKEAIECIKLFEMYSQ"
     gene            complement(1925..2008)
                     /locus_tag="PLY81_03025"
     tRNA            complement(1925..2008)
                     /locus_tag="PLY81_03025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1972..1974),aa:Leu,seq:caa)
     gene            complement(2036..2545)
                     /gene="hscB"
                     /locus_tag="PLY81_03030"
     CDS             complement(2036..2545)
                     /gene="hscB"
                     /locus_tag="PLY81_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNF29233.1"
                     /translation="MNYFELFNIPISFNPNLSDVKNKFYALSRKFHPDFFTQATQDEQ
                     QYALEQSALINKAYKILNNQESVLKYILELNNLIEDEEKYKLPPNFLMQVMDLNEQLL
                     DAKLEQDESKVNLVVNEINNFKTSIYEPVKSLLENCKEIIYSREELLQIKDFYYKKKY
                     LNRILAGLH"
     gene            complement(2608..3423)
                     /gene="ispE"
                     /locus_tag="PLY81_03035"
     CDS             complement(2608..3423)
                     /gene="ispE"
                     /locus_tag="PLY81_03035"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNF29234.1"
                     /translation="MIVFPNCKINLGLNIISKREDGFHNLETIFYPLMIKDCLEILPT
                     HKKENNFTITGIPINVDSSQNICSKAWQLLKLNFPQLSNIQLHLHKTIPTGAGLGGGS
                     ANGAFTLQLLNKLFQLQLTTNQLINFALQLGSDCPFFIINTPCYSTSRGEQLQPINLS
                     LSNYYFVIVNPQIHINTATAFSTIQPIKPQKSVSEIIHQPIETWKEDLVNDFEKNIFL
                     QYPAIEKIKINLYQQGAVYASMSGTGSTVYGIFERESSFVNYFPSNYFVQQVI"
     gene            complement(3462..4046)
                     /locus_tag="PLY81_03040"
     CDS             complement(3462..4046)
                     /locus_tag="PLY81_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNF29235.1"
                     /translation="MKKIELEIIALSHSMTQSNSYAVVLGEISGIRRLPIVIGAFEAQ
                     AIAVALESIHPSRPLTHDLMKNFMTAFGVSLQEVIIHDLQEGIFFSKLICYSTNETIE
                     IDSRTSDAIALAVRFGCPIYTTSNVIDSAGIVLDESSTKKKTIENITTDETVSSNDFS
                     NFTLEELQNLLNDVLEQEDYIKAIAIRDEINKRK"
     gene            complement(4131..4439)
                     /locus_tag="PLY81_03045"
     CDS             complement(4131..4439)
                     /locus_tag="PLY81_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNF29236.1"
                     /translation="MLKNKLSALRIIGFAEAASWLLLLLIAMPLKYIWMKPLAVKIVG
                     WIHGILFITYITLALLVAKEHKWKFSKLINAFISAFVPLGTYFFDSRLKKEQLEFNKP
                     "
     gene            complement(4484..5500)
                     /locus_tag="PLY81_03050"
     CDS             complement(4484..5500)
                     /locus_tag="PLY81_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29237.1"
                     /translation="MKIFCLILSTCFCIATKAQKLTHKNYNTLQELEKSITTYSKDMI
                     MAEEAIDRYRADSFFTRGLVKALKTTYSFYYNFDSINTISKLYAPDSSFKIFTWQLVK
                     DYSSIRQKGAIQINTPDGSLKLFPLFDASDDMENPVDSTRNTTRWVGALYYKIILTTF
                     NNKKYYTLLGLDENNEKSNKKWIEVVTFNKEGHPVFGGRYFQYPNDELKPAQPAFRFC
                     LEYKKDARARLTYDDEIGAIVFDHLISEEKKPENRNTLIPDGDFEAFRWIDGRWIHVN
                     KLFDYSVDMTGVDPTIGNPPVPKPIKDKKPSKKEQKEIEKFDPYKVNEKRNKKEDQRN
                     KEEY"
     gene            5691..6446
                     /locus_tag="PLY81_03055"
     CDS             5691..6446
                     /locus_tag="PLY81_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29238.1"
                     /translation="MKNWFLFLFLLCATILKSKAQTSFIEYQKKFPRMVNALKTKEDT
                     LKKQFKEQNLQWPPKQLYIRSFKYDSQLEVWVRSNNKENYTLFKTYKVCALSGNLGPK
                     RIQGDYQVPEGFYYITDFNPKSEYHMSLGLNYPNASDLLLSDSAKPGSEIYIHGKCIT
                     VGCIPIMDKPMEELYILSSYAKHSGEDFIPVHIFPIKYTNYKSAQKLKVVTEEDKEYQ
                     IFTTKLKEVFDYFELHKQLPIISVNKKGEYVIM"
     gene            6498..7013
                     /locus_tag="PLY81_03060"
     CDS             6498..7013
                     /locus_tag="PLY81_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29239.1"
                     /translation="MKKGIKILLSTLAITLFTLSVNAQTEWVSQKIGNKIIAKFPTAP
                     IEVQAGAVYSLTDADSTNFTATFVDLEAMGLDSATLAGLVETEEFSEQFKTGLTSQMP
                     GVEVTKSDITKWNDLVAYNIEGTNASKKEKVTFKCIFWGSKMFTFICTYRDSVGPKNK
                     DIYINSLALIK"
     gene            complement(7081..7683)
                     /gene="infC"
                     /locus_tag="PLY81_03065"
     CDS             complement(7081..7683)
                     /gene="infC"
                     /locus_tag="PLY81_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNF29240.1"
                     /translation="MALPFKANSGGRGKFNPRFNRNQEPEHRINERIRVPQVRLVGDN
                     VEVGIYSTQEALRIAQDLELDLVEISPQADPPVCKAIDYKKFLYEKKKKEKEMKANAK
                     QSEVKEVRFTPGTDDHDFDFKAKHAENFLKEGNKVKAYVQFKGRAIMFKERGELVLLK
                     FAERLTEFGQPETLPKLEGKRMFLMLTPKTNKKKKEEKPS"
     gene            complement(7734..>7901)
                     /locus_tag="PLY81_03070"
     CDS             complement(7734..>7901)
                     /locus_tag="PLY81_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNF29241.1"
                     /translation="AELARVPYMLVVGEKEATENKLSVRRQGKGDVGQLTIEEFTKSI
                     VEEIINRKSLN"
BASE COUNT         2622 a         1216 c         1101 g         2962 t
ORIGIN      
        1 ttaccattga cctaaaaggg gcaatgcctg tgcctgtgca aataagaaaa atatctttat
       61 caatattctc tggtagtaca aatttgcctt gtgggccacg caaagtaaat gttgagccaa
      121 cttctatatt attgaaaata taagttgtac ctagacctcc ttctaacaat acaataacta
      181 attcaattac gttggtacca tctggagaag atgcaatgga gtaactacgc caacgcttat
      241 ttttttgttc gtgaatgggt aaatctaaag ttacaaactg tcctgctgta aattcaaagt
      301 tagatagctc ttcaatttct atccaatacc ttcttgtatt agcagtttct tgttctatct
      361 ttattacctt accgattctc catggttcaa gcatggttat atattttgac acaatttatg
      421 ttacaaattg tttttataca actattttat ttttatttta taaaactatt ttttggcaat
      481 aaataaaaag actcctatat tatagtttct tttaaatttt atgaaaatga aaaacttatt
      541 tatcatacga cacgccaaaa gcagtactaa ttttttaaat aataacgatt ttgaaagacc
      601 attaaatgaa caaggaataa aagatgccgc tttgatggct aaaaaacttt taagtaaggg
      661 aattattata gatggaattg ttactagtac tgcaattaga gctgctacta cagcaaacta
      721 ttttgcaact gcttttgcaa ttaataaaaa taatttttgg aatgaaccaa cgctttataa
      781 tgccaataaa aaaacttttt acaccattat caataattgg caatttacat taaacaatat
      841 agctattttt tctcacaacc caggtataac tcattttgca aatagtgtta ccaattcaaa
      901 aattgacaat atgccgcctt gtggtgtttt tgctataaaa attaattgca ataagtggca
      961 aagttttgaa acctcagaaa aaaaattttg gtttttcgat tgcccggaaa aaatatggta
     1021 acaaattttt attgagaata catttcaaat aattttatac actctatagc ttcttttaca
     1081 tcatgcactc ttaaaatatt ggctccattt agtaaacaaa ttgtatgtaa agcagtggta
     1141 ccattaagtg cttgctctgg tgtaactcct aatgttttat aaatggttga ttttcttgaa
     1201 acgcctatca atattggttt ctctaaaata gaaaaaacag acaactcttt tagtaactga
     1261 aaattatgtt gaattgtttt accaaatcca aagcctacat ctataattac atcttttatt
     1321 cccgcttgtt tagcttgatt tattttatct ataaagaaag ataaaacttc ttgtgttaca
     1381 ttatcgtaaa taggattttg ttgcatggtt tgtggtgtac cttttatatg catacacaca
     1441 taaggcacat tcatattagc cacagtagcc atcatatttt tatcaaatgt gccaccactt
     1501 atatcattaa caatagatgc tcctgcctct actgccttgc ttgcaactgt tgcattataa
     1561 gtgtctattg atatgattaa gttgctaaat ttttttttaa ttgcttctat tacatttact
     1621 gttcttctca attcttcctc tactgtaatt ggaaggctat ttggtttggt agattggcca
     1681 ccaatatcaa ttattatagc tccctcttgt accatttgct ctaccttaaa caaagcagaa
     1741 tctattgagt tttgttggct attggtataa aaagaattag gagaagtatt aataataccc
     1801 attactatag gtttagttat agtaagcaat tctcccttac aatttaatgt aaacattcaa
     1861 aaaaaatttt attaaagata ggggcaaata aaaaaaactt ctctagtaaa taaagaagtt
     1921 tttgtgccca gaacaggaat cgaacctgca catcattgct gataccagat tttgagtctg
     1981 acgcgtctac cagttccgcc atctgggcat ttgatgggtt gcaaatataa tagcatcaat
     2041 gcaaacctgc caaaattcgg ttaaggtatt ttttcttgta ataaaaatct tttatttgca
     2101 acaactcttc tctcgaataa ataatttctt tacaattttc caaaaggctt ttaacaggtt
     2161 cataaatgct tgttttaaaa ttattaattt cattaaccac aaggtttact ttactttcat
     2221 cctgttctaa ttttgcatct aataattgct cattcaaatc cattacttgc attaaaaaat
     2281 taggtggtag tttgtatttc tcttcatctt ctattaaatt gtttaattct aatatatact
     2341 tcaatactga ctcttgattg tttaaaattt tatacgcttt attaattaag gcagattgtt
     2401 ctaatgcata ttgttgttca tcttgtgttg cttgggtaaa aaaatctgga tgaaatttcc
     2461 tactcaatgc ataaaactta ttttttacat cagataaatt gggattaaag gaaattggta
     2521 tattaaataa ttcaaaataa ttcatacata aatttatatc agcaaagtta aacttacaag
     2581 gttgttttaa tttttgattt gcaaaattta tattacttgc tgtacaaaat agttggaagg
     2641 gaaataattt acaaaactag attccctttc gaaaatacca tatacagtgc ttcctgtacc
     2701 actcatactt gcataaactg caccttgttg gtataagtta attttaattt tctcaattgc
     2761 agggtactgc aaaaaaatat ttttttcaaa gtcatttact aaatcttctt tccaggtttc
     2821 tattggttga tgaataattt ctgaaacaga tttttgaggc tttataggtt gtatagtaga
     2881 aaatgctgtt gctgtattaa tatgaatttg aggattaaca attacaaaat agtagttact
     2941 taagcttaaa ttaattggtt gcaactgctc tcctcttgaa gttgaatagc aaggtgtatt
     3001 tataataaaa aaagggcaat cgctacctaa ttgtaaagca aaatttatta attggttagt
     3061 agttaattgc agttgaaata acttattcag taattgcaaa gtaaaggcac cattggcact
     3121 gccaccacct aagcctgcac ctgttggtat ggttttatgt aagtgtagct gaatattgga
     3181 tagttgtgga aagtttaatt ttaataactg ccatgctttt gagcaaatat tttgagatga
     3241 atctacatta attggtatgc cagttattgt aaagttattt tcttttttgt gagtaggtaa
     3301 aatttctaaa caatctttaa tcattaatgg ataaaaaatt gtttccaggt tatgaaaccc
     3361 atcttctctt ttactaatta tatttaagcc taaattaatt ttgcaatttg gaaaaactat
     3421 caaaatgata cttattaatg attaggtaac taaatagttt tttattttct tttatttatt
     3481 tcatctctta tagcaatagc tttaatataa tcttcttgtt ctaaaacatc gttcaataaa
     3541 ttttgtaatt cttctaaagt aaaattgcta aaatcattac tactaactgt ttcatcagtt
     3601 gtaatatttt ctattgtttt ctttttagtg ctgctttcat ctaaaactat accagcacta
     3661 tctataacat tacttgttgt ataaatgggg cacccaaatc ttacagctaa tgctattgca
     3721 tcgcttgttc tactatcaat ttcaattgtt tcattggttg aataacaaat taatttagaa
     3781 aaaaatattc cttcttgcaa atcatgaata attacttctt gaagcgatac tccaaaagca
     3841 gtcataaaat ttttcattaa atcgtgagtg agaggcctgc tagggtgaat actttctaaa
     3901 gcaactgcaa ttgcctgagc ttcgaatgca ccaattacaa ttggcaaacg gcgtattcca
     3961 ctaatttctc ctaatactac tgcataagag ttagattggg tcatactatg gcttaatgca
     4021 ataatttcta attcaatttt cttcataaat aaaaacagtt tacccttgct tggtaataaa
     4081 attccagtat caacaaaatt aaggtgttct attgtttaac caagtagtat ttaaggctta
     4141 ttaaattcaa gctgttcttt ttttagtctt gaatcaaaaa agtaagtacc caagggcaca
     4201 aaagctgaaa taaaagcatt tattaatttg ctaaatttcc atttatgttc tttggcaact
     4261 aatagtgcca aagtaatgta cgttataaac aaaattccat gtatccaacc aacaattttt
     4321 actgccaaag gcttcatcca aatatatttt aatggcattg caataagtaa taataataac
     4381 caagatgctg cctcggcaaa gccaattatt cttaaagcag agagcttatt ttttagcata
     4441 agatgttaga ttttttgttt aaaatgttta cttaagtgta gtattagtat tcttctttgt
     4501 tacgttggtc ttctttttta tttctctttt cgtttacttt atacggatca aatttctcaa
     4561 tttctttttg ctccttttta gaaggttttt tatctttaat tggcttaggc acaggaggat
     4621 tgccaatggt aggatcaaca cctgtcatat ctacactata atcaaataat ttgtttacat
     4681 gtatccatct tccatctatc catctaaatg cttcaaaatc tccatcgggt ataagcgtat
     4741 ttctattttc tggttttttt tcttcgctaa ttaaatggtc aaaaacaatg gcaccaattt
     4801 catcatcata agttaatctt gctcttgcat cctttttgta ttctaaacaa aatctaaaag
     4861 caggttgtgc aggttttaat tcatcgttag gatattgaaa atatctacca ccaaaaacag
     4921 gatgtccttc tttattaaat gttacaactt caatccattt cttattactt ttttcattat
     4981 tctcatctaa gcctaataaa gtataatatt tcttattatt aaatgtggta agaataattt
     5041 tatagtataa agctcccacc catcttgttg tatttctggt ggaatctacc gggttttcca
     5101 tatcatcgga tgcatcaaat aatggaaata attttaaaga gccatcgggt gtatttattt
     5161 gtattgcacc tttttgacga atggagctat aatcttttac taattgccaa gtaaatattt
     5221 taaagctact atctggtgca tatagttttg aaatggtatt aatagaatcg aaattataat
     5281 aaaatgaata ggttgttttt aatgctttta ccaaccctct tgtaaaaaaa ctatctgccc
     5341 tatatctgtc tatagcttcc tcagccataa tcatatcttt tgaataagtt gtaatacttt
     5401 tttctaattc ttgtaatgta ttataatttt tatgggtaag cttttgtgcc ttggtagcaa
     5461 tacaaaaaca agtactaaga attaaacaaa aaattttcat gaagttaaaa aaatagaata
     5521 ctaaattagt ttattgtttt tactttacta gtaataaaaa ttaaatgttt aaactttaag
     5581 ttttctttat cataacccga gatttgaaat gaaagttgaa agctaatttt taaatgttaa
     5641 tttacacctc tatatatttt tttattactt tataaagtat ttttgagccc atgaaaaatt
     5701 ggtttttatt tttgttttta ttgtgtgcaa ctattcttaa aagtaaagca caaacctctt
     5761 ttattgaata ccaaaagaag tttcctagaa tggttaatgc tttaaaaacc aaagaagata
     5821 ctcttaaaaa gcaatttaaa gaacaaaatt tacaatggcc gcccaaacaa ttatatataa
     5881 gaagttttaa gtatgactct caattagaag tatgggtaag gagtaataat aaagaaaact
     5941 acacactttt taaaacctat aaggtttgtg cattaagcgg aaatttaggc cctaaaagaa
     6001 ttcaaggcga ttatcaagtg cctgaaggtt tttattacat tacagatttt aaccctaaga
     6061 gtgaatatca tatgagtttg ggcttgaatt atcctaatgc atctgattta ttacttagcg
     6121 attctgccaa acctggaagt gaaatttaca ttcatggaaa gtgtattact gtaggctgta
     6181 ttccaataat ggataagcca atggaagaat tatacatact cagttcttat gcaaagcata
     6241 gtggcgaaga ttttattcct gttcatattt ttcctataaa atatactaac tacaaaagtg
     6301 ctcaaaaatt aaaagtagtt acagaagagg ataaggaata tcaaattttt actactaaac
     6361 tgaaagaagt atttgattat tttgaactac ataagcaatt accaattata tctgtaaata
     6421 aaaaaggtga atatgttatt atgtaatgct tattacatta atattgtgcc attattcaat
     6481 tcaaattttt aaaaaacatg aaaaagggga ttaaaatatt gttaagcaca ttagctataa
     6541 cattatttac attaagtgta aacgcacaaa ctgaatgggt atctcaaaaa attggcaaca
     6601 aaataattgc aaaatttcct acagcaccaa tagaggtaca ggctggtgct gtatatagtt
     6661 taacagatgc tgactctacc aatttcactg ctacatttgt agatttggaa gcaatgggtt
     6721 tagactctgc aacacttgca ggattggtag aaacagaaga attctctgag cagtttaaaa
     6781 ctggtttaac tagtcaaatg ccaggtgtag aagttacaaa atctgatatt acaaaatgga
     6841 atgacttagt agcttataat attgaaggca ctaatgcttc taaaaaagaa aaggtaactt
     6901 ttaaatgtat tttttgggga tcaaaaatgt ttacatttat ctgcacctat cgtgatagtg
     6961 tagggccaaa aaataaagat atatatataa actctttagc gttgattaaa tagtttttat
     7021 agttaatcca taaaaaaacc tctccaaaag gagaggtttt tcttttttat ttaaccctta
     7081 ttaacttggt ttttcttctt tcttcttttt atttgttttt ggtgtaagca ttaaaaacat
     7141 tcttttacct tctaattttg gtaaagtttc tggctgacca aattcggtta atctttcagc
     7201 aaattttaat aatactagtt cgcctctttc tttaaacata atagcacgtc ctttaaactg
     7261 tacataagcc tttaccttgt ttccttcttt taaaaaattt tctgcatgtt ttgctttaaa
     7321 atcaaaatca tgatcgtctg tacctggtgt aaaacgtact tcttttactt cactttgctt
     7381 agcattagcc ttcatttctt tttccttctt ctttttttca taaagaaatt ttttgtagtc
     7441 tattgcttta caaacaggag gatcagcctg aggtgaaatt tcaaccaaat ctaattctaa
     7501 atcctgagca attcttaatg cctcttgtgt agaataaata ccaacttcta cattatctcc
     7561 tactaaacga acttgaggta cacgtattct ttcattaata cgatgttctg gttcttgatt
     7621 tctattaaaa cgtgggttga atttacctct gcctccagag tttgctttaa atggtaatgc
     7681 cattaaaatt tatttggtta aaaattgctg gtttaaaatt aattaatatt tgattaattt
     7741 aatgattttc tatttattat ttcttctaca atgcttttag taaactcttc tattgttagt
     7801 tggcctacat ctccttttcc ttgcctacga acagagagtt tattttctgt tgcttctttt
     7861 tcgccaacaa caagcatata aggtacacga gctagctctg c
//
LOCUS       DAOQHP010000057         7786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4865_length_7786_cov_3.450912, whole genome shotgun sequence.
ACCESSION   DAOQHP010000057 DAOQHP010000000
VERSION     DAOQHP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_7786_cov_3.450912"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PLY81_03075"
     CDS             <1..1833
                     /locus_tag="PLY81_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29242.1"
                     /translation="NTKPATGQLYRFTPTDSCNGQPSSGNANASILATCANVPFNLNL
                     TAFTSTKGISFQWQSSGIGQNIWVNIPGADSNFVTTTQTNASDYRCIVTCTKSALNDT
                     SNVLTVGQIAAIACTLENDECATATTITQNAYNNCTGTLFNTSLASLSANTSTFYTGS
                     QEDDIWFKFIATNDKAVIRFFDITAVSGTLGNMQFAFYTGTNCNTLTDLGGRTVQINN
                     NEGEVLLYGLTLGNTYFVRVATEGTNWRAIGKICIAEPAIAIGSNNNCISLTTTESTI
                     SNHNSWIPIYDGAKLVAEINAQGNTMGAITANLFTTDIVRTFAGNGRPYLSRNIQITP
                     AIQPSSNVKVRLYILYAEITLLASNPSSLVNTINDLYITKNNDACLPNYSGAGTFIIP
                     TARVNYANGGYIEFETSSFSSFYLHGGLGVLPITIQYFNGKKDGNYNLLQWQTINELH
                     TTGFEVQRSTDGKYFSTIAMVPTKATNNNIDKTLQYSFNDYKPLFNSYYRLKQTDKNG
                     AVSYSNIVFISSAFINQTQLIGIYPNPVKDVCKVLLFIPTTTNISLVLTDITGKIITQ
                     KNTTYLQGNIEEKISMGQLLNGIYLLKITDINGNLISIQKIVKQ"
     gene            1861..2508
                     /gene="nth"
                     /locus_tag="PLY81_03080"
     CDS             1861..2508
                     /gene="nth"
                     /locus_tag="PLY81_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNF29243.1"
                     /translation="MNKKERYQFVIDYFQEFMPVAETELIYDNPYQLLVAVILSAQCT
                     DKRVNQTTPAIFKKYPHPQSLSEASFQDLFALIKSISYPNNKTKHLIGMAQMLMNKFN
                     GEIPLTVNELIQLPGVGRKTANVITSVIDNQPNMAVDTHVFRVSKRIGLVSPKANTPL
                     AVEKELIKNLPTELIYKAHHWLILHGRYICLARSPKCNECGIATACLYYAKLLKQ"
     gene            complement(2521..3492)
                     /locus_tag="PLY81_03085"
     CDS             complement(2521..3492)
                     /locus_tag="PLY81_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29244.1"
                     /translation="MKFTNLKYVLLFAIIILLGSCFKETSYEKGANVYGFSSGTLKDS
                     LGDCKGIVIKGAYQAGTVLTDSNYIIVGVNITQSGKYVIYSDTVNGFWFRDSGYVAVG
                     TNTIKIKAFGTPVSNVNADFVVTYNNSVCLFSITAGIVAPLPIISDYFPRSIGSNWGY
                     NVLGLDSLHTKSTPKDTILGGNSYRVFYANGSLDDTAFYRKSGGDYYRWDALDGNTGY
                     MPLLFLKEDKPAGTQWESNIANTVYSGIPTQVKMKYTLMSVNVSRTVESNTFDSVIHV
                     KNELQYKILGTFQTIQTMNTYFAKNVGLIEFEAAGIYQQKLRRWKVY"
     gene            3825..5330
                     /gene="atpD"
                     /locus_tag="PLY81_03090"
     CDS             3825..5330
                     /gene="atpD"
                     /locus_tag="PLY81_03090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNF29245.1"
                     /translation="MASKGTVKQVIGAVVDVHFPNDNALPDIYNALELTRENGDKLVL
                     EVQQHLGEDSVRTIAMDGTEGLVRGMEVIDTGNAIKMPIGEAINGRLFNVTGDAIDGL
                     PQLNKSNGRPIHAKPPSFENLNTATEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGKT
                     VLIQELINNIAKGHGGLSVFAGVGERTREGNDLLREMIEAGIMKYGDGFKHSMEEGGW
                     DLSKVDLEGLKESKATFVFGQMNEPPGARARVALSGLTIAEYFRDGDGSGKGKDILFF
                     VDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKHGSITSVQAVYV
                     PADDLTDPAPATTFAHLDATTVLSRKIADLGIYPAVDPLDSTSRILTPAVVGEAHYNT
                     ANRVKLILQRYKELQDIIAILGMDELSDDDKMTVSRARKVQRFLSQPFHVAEQFTGLK
                     GVFVNIDDTIRGFNAIMDGEVDEYPEAAFNLVGTLEDAIEKGKKMMAQAHG"
     gene            5372..5623
                     /gene="atpC"
                     /locus_tag="PLY81_03095"
     CDS             5372..5623
                     /gene="atpC"
                     /locus_tag="PLY81_03095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNF29246.1"
                     /translation="MTLEILTPEKKLYSGDVYGVQLPGINGLFEVLDKHAPLVSALNK
                     GKIKILKSKTSFEYYTIQKGFVEVLNNKATVLVEGAVEA"
     gene            5701..6240
                     /locus_tag="PLY81_03100"
     CDS             5701..6240
                     /locus_tag="PLY81_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29247.1"
                     /translation="MQQLGIGSRVEHTHFGKGVIVDIGSEFYTIWFKLQNTTKSIGKD
                     FELKVIEATENTQQLNNISLADIELALNNILEQRLHEFQLVPMANKWQNGNLILQPAD
                     ATLQPKEIPIETFFHKIVMVRDRLRLIEQKINANKVLTDEEKIDLQQYITAIYGSLTT
                     FNVLFKEAHHQFKGMSTKD"
     gene            6428..6727
                     /locus_tag="PLY81_03105"
     CDS             6428..6727
                     /locus_tag="PLY81_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNF29248.1"
                     /translation="MNIKIQAVRFNADQKLIDLVARKVEKLKTYHDKIIQVDVFLKLD
                     NVVHNIKDKIAEIRVTIPRHEFFVKASSKSFEESFENALLSISNQIKRKKEKLAA"
     gene            6839..>7786
                     /gene="holA"
                     /locus_tag="PLY81_03110"
     CDS             6839..>7786
                     /gene="holA"
                     /locus_tag="PLY81_03110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNF29249.1"
                     /translation="MSADKIISSWMKGKYAPVYFLDGEEPYFIDKIINYAETKIINEN
                     ETAFNLSIFYGRDSNWADIVNACKRYPMFADKQVVIIKEAQHLKEIDKLENYIIHPLP
                     STILVIAYKDKKLDARTKFAKTIKEKAEIFTSKKLNDNEVINWTSKIIEDYNLKISQS
                     ALHLLVEHIGNNLVRIANEIEKLSITLRNDKTITEDAIELYIGISKDYNEFELQKVIG
                     RKDFTKAMRIVQYYEKNPTQTPIQLILISLCNYFSKVYALYTIGNNNENTIATTLGIH
                     PFYVKDYIYSTRIYQTQEVEKILLLLNKYALKSVGVDNAS"
BASE COUNT         2858 a         1218 c         1238 g         2472 t
ORIGIN      
        1 aatactaagc ctgccactgg gcagttatac aggtttaccc caacagattc ttgtaatggg
       61 caacctagta gtggtaatgc caatgcatct attctcgcta cttgtgccaa tgtgccattc
      121 aatcttaact taactgcttt tacaagcaca aaaggtattt cttttcagtg gcaatcttct
      181 ggtatagggc aaaatatttg ggtaaatatt cctggagccg attctaattt tgtaacaact
      241 actcaaacca atgcaagtga ttatagatgt attgttactt gtaccaaatc ggctttaaat
      301 gataccagta atgtacttac agttgggcaa attgctgcaa ttgcttgtac tcttgaaaat
      361 gatgaatgtg caactgccac aactattaca caaaatgcct ataacaattg tactggtact
      421 ttatttaata ccagccttgc tagtttatct gctaatacct ctacctttta tacaggaagc
      481 caggaggatg atatttggtt taaatttatt gcaactaacg ataaagctgt tataagattt
      541 tttgatatta ctgcagttag tggcacattg ggtaatatgc aatttgcttt ttatacaggt
      601 acaaattgta atacattaac tgatttaggc ggacgcactg tacaaattaa taacaacgaa
      661 ggagaggttt tgctatatgg tttaaccctt ggcaacactt attttgtaag agttgccaca
      721 gaaggaacaa actggagagc cataggaaaa atttgtattg cagaaccagc aattgctatt
      781 ggtagtaata ataattgcat ttcgctaact acaacagaaa gtacaattag caaccataat
      841 agctggattc ctatttatga tggagcaaaa ttagtagccg aaataaatgc tcaaggaaat
      901 acaatgggtg ctataactgc caatttattt actaccgata ttgttagaac atttgcaggc
      961 aatggtaggc cctatttaag tagaaacata caaataacac cagccataca acccagtagt
     1021 aatgtaaaag ttagattata tattttatat gccgaaataa cacttttagc atctaatcca
     1081 tcgtctttag taaatactat taacgatttg tacataacta aaaacaacga tgcatgttta
     1141 cccaattact ctggtgcagg cacgtttata atacctactg caagggtaaa ttatgccaat
     1201 ggtggttata ttgaatttga aacttcttcc ttctcttctt tttatttaca tggaggctta
     1261 ggtgttttac caattaccat tcaatatttt aatggaaaaa aagatggcaa ctacaattta
     1321 ttacaatggc aaacaataaa cgaattacat actactggat ttgaagtaca aagaagtact
     1381 gatggaaaat atttttctac cattgcaatg gtacctacca aagcaaccaa caacaatata
     1441 gataaaactt tacaatatag ctttaacgat tacaaacctt tatttaatag ctattacaga
     1501 ctaaaacaaa cagataaaaa tggggctgtt tcttattcaa atattgtatt catttcatct
     1561 gcctttatta accaaacaca attaattggc atttatccaa accctgtaaa agatgtatgt
     1621 aaagtgctat tatttattcc aacaactacc aatatttctt tagttttaac agatattaca
     1681 ggcaaaatta ttacacaaaa aaatactact tacttacaag gcaatataga agagaagata
     1741 agtatggggc aattattaaa tggtatatac ttgttaaaaa ttacagacat taatggcaac
     1801 ctgattagca ttcaaaaaat agtaaaacaa taagtaaaaa aaattactat tttatataga
     1861 atgaataaaa aagaaagata ccaatttgta attgattact ttcaagagtt tatgccggtg
     1921 gcagaaacag aattgattta cgataatcct tatcaattat tagtagcagt aattttatcg
     1981 gctcaatgta cagataaaag ggtaaaccaa acaacgcctg ccatttttaa gaaatatcct
     2041 catccacaat ccttgtctga agccagtttt caagatttgt ttgctttgat aaaaagtatt
     2101 tcttacccca acaataaaac taaacactta ataggcatgg cacaaatgct tatgaataaa
     2161 tttaacggag aaataccttt aactgtaaat gaattaatac aattaccagg agtaggaaga
     2221 aaaacagcca acgtaattac cagtgttata gataaccaac ctaatatggc agtagataca
     2281 catgtgttta gggttagtaa acgtataggc ttggttagcc caaaagcaaa tacaccttta
     2341 gctgtagaaa aagaattgat taaaaactta cctaccgaac taatttataa agcacaccat
     2401 tggctaattt tacacggaag atatatttgt ttggcacgtt ctccaaaatg taacgaatgt
     2461 ggtattgcca ctgcctgctt gtattatgct aaactattaa agcaataaac attttatagt
     2521 ttaatacact ttccaccttc ttaatttttg ctgataaata cccgcagcct caaattcaat
     2581 tagccctaca tttttggcaa aataggtatt catagtttgt atggtttgaa aagttcctaa
     2641 aattttatat tgcagttcat tttttacatg aattacacta tcaaaagtgt tgctttcaac
     2701 agttctgcta acatttacac tcattaaggt atatttcatt tttacttgtg taggaatgcc
     2761 actatatact gtattagcaa tattactctc ccattgggtg cctgctggtt tatcttcttt
     2821 taaaaacaat aaaggcatat atccagtatt gccatctaaa gcatcccacc tataataatc
     2881 tccaccagat tttcggtaaa atgcagtatc atctaaacta ccattggcat aaaaaaccct
     2941 ataagaattt cctcctaaaa tagtatcttt tggcgtagat ttagtatgta acgaatctaa
     3001 gcctaacaca ttataacccc agttactacc tatagatctt ggaaaataat cgctaataat
     3061 tggtaaagga gcaacaatgc ctgcagttat tgaaaataaa caaaccgagt tattgtaagt
     3121 tacaacaaaa tcggcattta cattgcttac aggagtgcca aatgctttta tcttaatagt
     3181 attagtgcca acagcaacat agccactatc tctaaaccaa aatccattta ccgtatcaga
     3241 ataaataaca tatttacccg attgtgtaat attaactccc acaataatgt aattactatc
     3301 cgttaaaaca gtaccagctt ggtaagcacc ttttattact attcctttac aatctcccaa
     3361 actgtctttt aaagtacccg aagaaaagcc atacacatta gccccttttt cgtaacttgt
     3421 ttctttaaaa caagagccta aaaggataat tattgcaaat agcaatacat atttaaggtt
     3481 agtaaatttc atttatctgt ttttctgatt gtaaaaaact gttttattaa aaaaatattg
     3541 ttccaaaaat atttcaaaac cgaatatgta gctataatta ttcaagtttt tagataaacc
     3601 ttagtaatta cttgctgcct aacctaaaat taaattaata aaaactataa tcattatata
     3661 aaagttaggg ttaatataca ggtattatta tgaaaaagct ataaatacta tttttccgta
     3721 cttattcaca ctataaaaat tcctttaaaa acatggtata tttagttttt tgaaagattg
     3781 ttacccctta cctttgccgc caaatacaaa aatcataatt aattatggct tctaaaggta
     3841 cagttaaaca agtgataggt gcagtagtag atgtgcactt tccaaacgat aatgcattgc
     3901 ccgatattta caatgctctt gaacttacaa gagaaaatgg ggataaatta gtgttagaag
     3961 ttcaacaaca cttaggcgaa gacagtgtac gtactattgc aatggatggt acagaaggtt
     4021 tagtacgtgg tatggaagta attgatacag gcaatgccat taaaatgcca attggcgaag
     4081 ctattaatgg tcgtttattt aatgttacag gagatgcaat tgatggttta ccacaattaa
     4141 ataaaagcaa tggaagaccc attcatgcta aacctccaag ttttgaaaat ttaaatacag
     4201 caacagaagt actatttaca ggtattaaag taattgacct aattgagcct tatgcaaaag
     4261 gtggtaaaat tggtttattt ggtggtgctg gtgtaggtaa aacagtatta attcaagagt
     4321 tgattaataa tattgctaaa ggccatggtg gtttatcggt atttgcaggt gtgggcgaaa
     4381 gaacacgtga aggaaatgat ttattaagag aaatgattga agccggcatt atgaaatatg
     4441 gcgatggctt taaacatagt atggaagaag gtggctggga tttaagtaaa gtagatttag
     4501 aaggcttaaa agaaagtaaa gcaacttttg tatttggaca aatgaacgag cctcctggag
     4561 caagagcaag agtggcttta agtggtttaa ctattgctga atattttcgt gatggagatg
     4621 gctctggcaa aggaaaagac attctatttt tcgtagataa tatcttccgt tttacacaag
     4681 ctggctcaga agtttcggct ctattaggtc gtatgccttc tgcagtaggt tatcagccaa
     4741 ctttagcaac agaaatgggc ttaatgcaag aaagaattac ttctactaaa catggctcaa
     4801 ttacatctgt acaagctgtt tatgtgcctg cagatgactt aactgaccct gctcctgcta
     4861 ctacctttgc tcacttagat gctactacag tattaagtag aaaaattgcc gacttaggaa
     4921 tctatccagc agttgaccca ttagactcta cctctagaat tttaacccct gctgtagtag
     4981 gagaagccca ctacaacact gctaatagag taaaattaat attacaacgc tataaagagc
     5041 tacaagatat tattgctatt ttgggtatgg atgaattgag tgatgatgat aaaatgactg
     5101 tttctcgtgc aagaaaggta caacgtttct tatctcaacc atttcatgtg gcagaacaat
     5161 ttacaggctt aaaaggtgta tttgtaaata ttgacgatac cattagaggc tttaatgcaa
     5221 ttatggatgg cgaagtggat gaatatccag aagctgcatt taaccttgta ggtactttag
     5281 aagatgccat tgaaaaaggt aaaaaaatga tggctcaggc tcacggataa ttggtatttt
     5341 aatgattaaa ttttaaagta aatacaaaaa tatgacttta gaaatattaa caccagaaaa
     5401 aaagctttat agtggagatg tgtatggggt acaattacct ggtattaatg gtttatttga
     5461 agtgttagat aaacatgcac ctttagttag tgccttaaat aaaggaaaaa ttaaaatctt
     5521 aaaatctaaa acaagttttg aatactatac tattcaaaaa ggctttgtag aggttttaaa
     5581 taataaagca actgtactgg tagaaggtgc tgtagaagca taaaataata taacttatct
     5641 tatccttaaa atctctctgc actaagagag atttttttat tttctttaca acaaaaaatt
     5701 atgcaacaat taggtattgg atcaagagta gaacatacac attttggcaa aggtgttatt
     5761 gtagatattg gatctgagtt ttatacaatt tggtttaaat tacaaaatac caccaaaagc
     5821 ataggcaaag attttgaatt aaaagttata gaagcaactg aaaatactca acaattaaac
     5881 aatatttcat tagccgatat tgaacttgct ttaaacaata ttttagaaca acgtttacac
     5941 gaatttcagt tagtgccaat ggcaaataaa tggcaaaacg gaaatttaat tctacaacct
     6001 gccgatgcta ccttacaacc aaaagaaata ccaatagaaa ccttctttca taaaatagta
     6061 atggtaagag ataggctaag actgatagaa caaaagatta atgcaaataa agtattaacc
     6121 gatgaagaaa aaattgattt acaacaatat attactgcaa tatatggttc cttaaccacg
     6181 tttaatgttt tgtttaaaga agcccatcat cagtttaaag gcatgagtac aaaagactaa
     6241 taaataccta ttaaaggtaa attattgcaa gccgtgaatt gaaaatttat tatttacgaa
     6301 attttaaaca ttttttagtt ttttattcag gttaaaaatg ctaacttgta accaataaaa
     6361 ataacgattg cctatgtaag ttttctaccc ttaaacattt ttattcacct ttaaaacatt
     6421 atttgatatg aacattaaaa ttcaagcagt tcgtttcaat gcagatcaga agttgattga
     6481 cttagtagct cgtaaggtag aaaaacttaa aacctatcat gataaaatta ttcaggtaga
     6541 tgttttctta aaactagata atgtagtaca taatattaaa gataaaattg cagaaataag
     6601 agtaaccatt ccacgtcatg aattttttgt gaaagcatct tcaaaatcat tcgaagaaag
     6661 ttttgaaaat gcactcttat ctatctctaa tcaaattaaa cgtaaaaaag aaaaactggc
     6721 agcatagcct gttgtaaaag cctgctttat ttggcaggct tttttatttt tatcaacagt
     6781 gtgctattaa ttaatataat ttgttcttcg cctactttgc actccttatt tttgttacat
     6841 gtctgccgat aaaattatta gctcttggat gaaggggaaa tatgcacctg tttatttttt
     6901 agatggggag gaaccctatt ttattgataa aataattaac tatgccgaaa ctaaaattat
     6961 caatgaaaat gaaacagctt ttaaccttag tattttttat ggaagagatt ccaactgggc
     7021 agatatagtg aatgcctgca aacggtaccc aatgtttgca gataaacaag ttgtgataat
     7081 taaagaagca cagcatttaa aagaaataga taaattagaa aactacatta tccatccttt
     7141 gccatcaacc attttagtaa ttgcttataa agataaaaaa ttagatgccc gaactaagtt
     7201 tgcaaaaaca attaaagaaa aagccgaaat ttttacaagt aaaaaactta atgataatga
     7261 agtaataaac tggaccagca aaattattga agattataat ttaaaaattt cgcaaagtgc
     7321 attgcattta ttggtagagc atattggaaa taacttagta cgcattgcta atgaaataga
     7381 aaagttatcc attactttaa gaaatgataa aaccattaca gaagatgcaa ttgaactgta
     7441 tattggaata agtaaagatt ataacgaatt tgaattacaa aaagtaattg gcagaaaaga
     7501 ttttaccaag gcaatgcgta ttgtacagta ctacgagaaa aaccctactc aaacacccat
     7561 acaattaata ttaataagcc tttgtaatta ttttagtaaa gtgtatgcac tatatactat
     7621 tggcaataac aacgaaaata ctattgccac tacccttggc attcatcctt tttatgttaa
     7681 agactatatt tattctaccc gtatatacca aacgcaggaa gtagagaaaa ttttattgtt
     7741 actaaataaa tatgcattaa aaagtgtagg tgtagataat gcaagt
//
LOCUS       DAOQHP010000058         7636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_4992_length_7636_cov_3.333861, whole genome shotgun sequence.
ACCESSION   DAOQHP010000058 DAOQHP010000000
VERSION     DAOQHP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4992_length_7636_cov_3.333861"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLY81_03115"
     CDS             complement(<1..1345)
                     /locus_tag="PLY81_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29250.1"
                     /translation="MKYLLNILAFGFALLLFNSANAQIITTSNKVVKKIASKSMFAFD
                     GRKIVPHTVIIYNYDSSFYSIDYTKNIINWLKPTASDSVLIEYRYFNFKINENAYRYN
                     YDSVINNFIAAPQVISQSKQNENVGLFGQTISINYNGSFGRNLSFGNNQDAVFNSQLN
                     LQISGMLGDSIEIAAAITDNNIPIQPDGTTQQLNEFDKILLQFKKRNMQLDLGDIDVR
                     SNQYYFLNFYKRLQGASYKSLNSKKNSNFSFAGAIAKGKFARNIFNGLEGNQGPYKLA
                     GNNNEFFLIILAGTERVFMDGEMLQRGEDQDYIINYNTAEIVFTPKHMITKDKRIQVE
                     FEYADRNYLNYLVYAAKEMKVNKNLQINIAAYSNNDAKNSPINQQLNANQKQFLNSLG
                     DSIEQAFYHSVVLDSFSNTKILYAKRVNPINALWDSIYVYSTHADSAKYYLNFIII"
     gene            1603..2814
                     /gene="sucC"
                     /locus_tag="PLY81_03120"
     CDS             1603..2814
                     /gene="sucC"
                     /locus_tag="PLY81_03120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNF29251.1"
                     /translation="MNLHEYQAKELLKKYNVPVQEGIAVESVMAAEDAYRQIKTQTNN
                     NFAVIKAQIHAGGRGKGKVNESGLNGVKVAKSLEDISTYAKGILGGTLVTIQTGPAGK
                     KVNKVLIAQDVYYPGPNPVKEFYLSILLDRAKGQNVIMYSTEGGMDIEEVAHNTPEKI
                     FKEWVHPSGGLQGFQARKIAFNLGLSGDAFKSCVKFVTNLYNAYVGLDCSMLEINPLF
                     KTSDDKIIAVDCKMGLDENALMRHPDLAALRDITEEDPTEVEAGQFNLNYVKLEGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFKIILKDPNVKAILINI
                     FGGIVRCDRVAAGVIEAYNKLGNINIPIIVRLQGTNAAEAKKLIDESGLKVQSAILLS
                     EAADLVKKALA"
     gene            complement(2969..5119)
                     /gene="fusA"
                     /locus_tag="PLY81_03125"
     CDS             complement(2969..5119)
                     /gene="fusA"
                     /locus_tag="PLY81_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF29252.1"
                     /translation="MADLKFQRNFGIAAHIDAGKTTTTERILRYTGMIHKIGEVHDGG
                     ATTDWMEQEKERGITITSAAVSCQWNFPTILGKATPETKKYYFNIIDTPGHVDFTVEV
                     ERSMRVLDGLIALFSAVDGVEPQSETVWRQANRYKVPRIGFVNKMDRAGADFLNVVKQ
                     VKEMLGAKSVPLQLPIGAEDNFKGVVDLIKMKGIVWHAETEGMTFDEIPVPEDMMEDV
                     KFWRQNLIEAVAEYDDKLMEKFFENPETITEEEVHEAIRKACIDLSIVPMMCGSSFKN
                     KGVQTALDAVCRYLPSPVDIEETIGTNPDTGEQEVRKADAKEPFSALAFKIMTDPFVG
                     RLAFFRCYSGHLDAGSYVLNVRSGKKERISRIMKMFANKQNPIDFIEAGDIAAAVGFK
                     EIKTGDTLCDEKAPITLENMFIPEPVIAIAIEPKTQADVDKMGMAIAKLIEEDPTLRV
                     NTDEDTGQTILRGMGELHLEIIIDRLRREFKVEVNQGAPQVAYKEAFHSTIEHREVLK
                     KQSGGRGKFADIQFEIGPADEEWIKENEGKSFQFVNDLFGGSIPKEFVPAIIKGFETS
                     MVTGVLAGYPVNNMKVRVFDGSYHDVDSDSMSFELCAKSGFREAGRKAKPVLLEPIMK
                     VEVLTPDQYMGDVTGDLNRRRGMLEGMDSRNNLQVIKAKVPLSEMFGYVTQLRSLSSG
                     RATSTMEFSHYTPAPNNIAEEVIAKNKGKVKVEE"
     gene            5412..6650
                     /locus_tag="PLY81_03130"
     CDS             5412..6650
                     /locus_tag="PLY81_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF29253.1"
                     /translation="MNKNERIILFLLACINFTHILDFMILMPLGNYLMPYFNITTTQF
                     SFLVGAYTLSAAFSGFTAAFFVDGFDRKKVLLFAYSGFLLGTLACGIAPTYHLLLAAR
                     ILAGLFGGLIGAQVLSIISDVFVYERRGKAMGAVMSAFAIASTFGVPFSLYLANLFSW
                     HAPFIFVAIVGTILIPLIQKYVPAIDHHLQNKSEKKSPFEALHIIREDRNLILALTFS
                     GLVMLGHFLIIPFINPYMEFNNGFSKQLTPMIYLVGGIASFISANILGRISDKYGKLK
                     VFSICIFISLPLVIGVTHVPAIPFWIILVMFAFWFTVATGRGVTAQAMISNAVQAKHR
                     GSFMSFNSSVQQLGTSIASLVAGVIVIKENDGKLHHYKWLGYLSVVVLLACLFLGRYI
                     FKKENYSTSQLSEDNISELI"
     gene            6742..7365
                     /locus_tag="PLY81_03135"
     CDS             6742..7365
                     /locus_tag="PLY81_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNF29254.1"
                     /translation="MNQKKFTILCLGDSYTIGENVPIHDSFPYQTIQLLRKKGCNFYA
                     PEIVAKTAWTSFELIEHLSGITLLENYDYVTLLIGVNNQYRGLEIEEFKKDFEWLINK
                     ALQLVGFNSNKVIVLSIPNWGVTTFAKDRDEKSITKAIESYNLICKQLAENNKTNFID
                     ITNHSLLAKDNQQLLAADGLHYSGLAMQEWAVMLANVISKDILTEKK"
     gene            7426..>7636
                     /locus_tag="PLY81_03140"
     CDS             7426..>7636
                     /locus_tag="PLY81_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29255.1"
                     /translation="MKFIFTCILWLFFYSIPLSAQYYLRGEIKDEKGKFLPNVKISLF
                     SKNNFRFSYTSGNSGSFGIPSTLEID"
BASE COUNT         2542 a         1238 c         1153 g         2703 t
ORIGIN      
        1 ttataataat aaagtttaaa taatattttg cactatctgc atgggttgaa taaacataaa
       61 tactatccca caaagcattg atggggttta ctcgttttgc atataaaatt tttgtattag
      121 agaaagaatc taaaacaaca gaatgataaa aagcttgttc aatgctatct cctaaactat
      181 ttaaaaattg tttttgattt gcattcagct gttggttaat tggagaattt ttagcatcgt
      241 tattactata tgcagctata ttaatttgta aatttttatt tactttcatt tcctttgcag
      301 cataaaccaa atagtttaaa taatttctat cggcatattc aaactcaacc tgaattcttt
      361 tatcttttgt aatcatgtgc tttggtgtaa acacaatttc ggcagtgtta taatttataa
      421 tataatcctg gtcttcgcct ctttgtaaca tttcaccatc cataaaaact ctctctgtgc
      481 ccgccagaat aattaaaaaa aattcgttgt tattacctgc taatttataa ggcccttgat
      541 tcccttctaa cccattaaaa atatttcttg caaactttcc ttttgcaata gcacctgcaa
      601 aagagaagtt gctatttttc ttactgttta acgatttata gctagcacct tgtaaccttt
      661 tataaaaatt caggaaataa tattgattac ttcttacatc aatatctcct aaatctaatt
      721 gcatatttct tttcttaaac tgaagcaaaa ttttatcaaa ttcattcaat tgttgcgtag
      781 ttccatctgg ctgaattggt atattgttat ctgtaattgc agcagcaatt tctatgctat
      841 ctcccaacat accacttatc tgcaaattca attgtgagtt aaaaactgca tcttgattat
      901 ttccaaagct taaatttctt ccaaaacttc cattgtaatt aatagatata gtttgaccaa
      961 ataaaccaac attttcattt tgtttggatt ggctgattac ttgaggtgca gcaataaaat
     1021 tatttattac actatcataa ttgtatctat aagcattctc atttatcttg aaattaaaat
     1081 accggtattc aattaaaaca ctatctgatg ctgttggctt taaccaatta ataatatttt
     1141 tcgtataatc aatactataa aatgaactat cataattata aattataact gtatgaggca
     1201 caatttttct gccatcaaaa gcaaacatac ttttagaagc aatttttttt acaaccttat
     1261 tagatgttgt tattatttgt gcattagcag agttaaacag taacaatgca aaaccaaaag
     1321 ccaatatatt tagtaagtat ttcaaaaata aagttggaaa gtaaaattta catcatacaa
     1381 aaattgtagt aataagttaa gtacaagata gtatttaggc ttaaacggct gcctaaattt
     1441 ataccagtaa agttttataa attatttaca attgtaataa attaagttat ttaaacataa
     1501 aaaatataca ataaaaattc agctttaatt ctctaatctt aataaagttt aaatcaattt
     1561 tactaaactt aacttatatt cgccgcataa aaataatcta ccatgaattt acatgaatac
     1621 caagctaaag aattattaaa aaagtacaat gtacctgtac aagaaggtat agctgtagaa
     1681 agtgtaatgg ctgcagaaga tgcttacaga caaattaaaa cacaaaccaa taacaatttt
     1741 gcagttatta aagctcaaat tcatgctggt ggcagaggaa aaggaaaagt aaatgaaagc
     1801 ggcttaaatg gagttaaagt tgccaaaagt ttagaagata ttagcactta tgcaaaagga
     1861 attttaggtg gcactttagt aaccatacaa acaggccctg ctggtaaaaa agtaaacaag
     1921 gttttaattg cacaagatgt ttattaccct ggcccaaatc ctgtaaaaga gttttattta
     1981 tctattttat tagatagagc taaaggtcaa aacgtaatca tgtattctac agagggtgga
     2041 atggatattg aagaagtagc acacaacact ccagaaaaaa tatttaaaga atgggtgcat
     2101 ccttctgggg ggttacaagg ttttcaggct cgtaaaattg catttaattt aggcttaagt
     2161 ggcgatgctt ttaaaagctg tgtaaaattt gtaactaatc tttacaatgc ttatgtgggg
     2221 ttagattgtt ctatgttaga aataaatcct ttatttaaaa caagcgatga taaaattatt
     2281 gctgtagatt gtaaaatggg attggatgaa aatgctttaa tgcgtcatcc tgatttagct
     2341 gcattaagag atattactga agaagatcca acagaagttg aagcaggcca atttaaccta
     2401 aattatgtaa aacttgaagg caatgttggt tgtatggtaa atggtgcagg cttagcaatg
     2461 gctactatgg atatgattaa attaagtggt ggcgaaccag ccaatttttt agatgttggt
     2521 ggtacagcaa atgcccaaac ggtagaagca ggttttaaaa ttattttaaa agacccaaat
     2581 gtaaaagcta tattaattaa tatttttggt ggcattgttc gttgcgatcg tgttgctgct
     2641 ggtgttattg aggcttataa taaattgggt aatattaata ttccaattat tgttcgttta
     2701 caaggtacaa atgctgcaga agctaaaaaa ttaattgatg aaagtggctt aaaagtacaa
     2761 agtgccattt tgttaagtga ggcagccgat cttgttaaaa aagcccttgc ataattaaat
     2821 tatttataca tgttttacta ccattagtta aaagctaatg gttttttttg agactaccaa
     2881 tcaaacaatt attaattacc attttcaaaa ataatccctg agccataagg ttcagggatt
     2941 ataatgatac tttataaaag taattgaact attcttctac ttttactttt cctttatttt
     3001 ttgcaattac ttcttctgca atattattag gagcaggagt gtaatgagaa aattccattg
     3061 tagaagttgc acgtccagat gataaagaac gtaactgtgt aacataacca aacatttcac
     3121 tcaacggaac ttttgcttta ataacttgta ggttatttct gctatccatt ccttctaaca
     3181 taccacgtct tctatttaag tcacctgtta catcacccat gtattggtct ggtgttaata
     3241 cttccacttt cataattggt tcaagtaaaa caggttttgc ttttctacca gcttcacgga
     3301 aaccagattt tgcacaaagc tcaaaactca ttgaatcaga atccacatcg tggtaactac
     3361 catcaaaaac acgaactttc atattgttta caggataacc tgctaaaaca cctgttacca
     3421 ttgatgtttc aaaaccttta attatagcag gtacaaattc ttttggaatt gaaccaccaa
     3481 ataaatcatt cacaaactgg aagcttttac cttcattttc tttaatccac tcttcatctg
     3541 ctggcccaat ttcaaattgt atatcagcaa atttacctct accacccgat tgttttttca
     3601 aaacttctct atgttcaatt gtagagtgga aagcttcttt ataagcaact tgtggggcac
     3661 cttggtttac ttcaacctta aactcacgtc ttaaacggtc aataataatt tccaaatgta
     3721 actctcccat tccacgtaaa atagtttgtc cagtgtcttc atctgtattt acacgtaatg
     3781 taggatcctc ttcaattagt ttagcaattg ccatacccat tttatcaaca tctgcctgtg
     3841 ttttaggctc tattgcaatt gcaattacag gttctggaat aaacatattt tctaaggtaa
     3901 taggtgcttt ttcatcgcac aaagtatcac ctgttttaat ttctttaaat cctactgcag
     3961 cagcaatatc tccagcttca ataaaatcaa ttgggttttg cttatttgca aacattttca
     4021 ttatacggct aatacgttcc tttttaccac ttcttacatt taatacataa gaaccagcat
     4081 ctaaatgacc agaataacaa cggaagaaag ccaaacgacc tacgaaagga tcggtcatta
     4141 ttttaaatgc aagagccgag aatggttctt tagcatctgc ttttctaact tcttgttctc
     4201 cagtatctgg gttagtacca attgtttctt caatatctac agggcttggc aagtatctac
     4261 aaacagcatc taaggcagtt tgtacacctt tatttttaaa cgaagatcca cacatcattg
     4321 gcacaatgct taaatcaata caagctttac gaattgcctc gtgaacttcc tcttctgtaa
     4381 tagtttcagg gttttcaaag aatttctcca tcaacttgtc gtcgtactca gcaactgctt
     4441 caattaaatt ttgtctccaa aatttcacat cttccatcat atcttctggt actggaattt
     4501 catcaaaagt cattccttct gtttcagcat gccaaacaat acctttcatt ttaattaaat
     4561 ctaccacacc tttaaaatta tcttcggcac caataggtaa ttgcaatgga actgattttg
     4621 ctcccaacat ttcttttacc tgcttaacta catttaaaaa atcggcacca gcacggtcca
     4681 ttttgttaac aaaaccaatt cttggtactt tataacggtt agcctgacgc caaactgttt
     4741 cagattgagg ctctacacca tcaactgcac taaataaggc aattaaacca tctaaaactc
     4801 tcatactacg ttctacctca actgtaaaat ctacgtggcc cggagtatca attatattaa
     4861 aataatattt tttggtttcg ggtgtagctt ttcctaaaat agtaggaaaa ttccattgac
     4921 agctaaccgc agcagaggta attgtaatac ctctttcttt ttcctgctcc atccagtcag
     4981 tagttgcacc accatcgtgt acttctccaa ttttatgaat catacctgta tagcgtaaaa
     5041 tacgctcagt agtggtggtt ttaccagcat caatgtgtgc tgcaataccg aagttgcgtt
     5101 gaaattttaa atctgccata acgcttgtat ttgtgtgttt taattttttt gtaatgggca
     5161 gttttacaat attcagcttc cgttgcgtcg cacacttcaa ctgtataaaa tatacccttc
     5221 aatttttttg agccgcaaag gtaggggttt agacatcttt tctacaaaaa gtatctttaa
     5281 aatatcaaaa aaaaaattac atatcaataa aaatagccat gttaaaataa cctgaaatta
     5341 attttattca tcaatttact agagaactta aatttttaaa acctactttg cagccctttt
     5401 tgcaaattat tatgaataaa aacgaacgta taattttatt tttactagcc tgtataaatt
     5461 ttacccatat tctagatttt atgatattaa tgccattagg caactaccta atgccttatt
     5521 ttaatattac aaccactcag tttagttttt tggtgggagc atatacatta agtgcagctt
     5581 tctctggttt tactgcagct ttttttgtgg atggtttcga cagaaaaaaa gtattgttat
     5641 ttgcttattc tggttttctt ttaggcacat tagcttgtgg tattgctcct acttaccatt
     5701 tattacttgc tgcaagaatt ttagctggct tatttggtgg cttaattggt gcacaagtat
     5761 tatccatcat ttccgatgtg tttgtttatg aaagaagagg aaaagctatg ggtgctgtaa
     5821 tgagtgcttt tgctattgca agtacttttg gagttccttt ttctttatac ttagccaatc
     5881 tttttagttg gcatgctcct tttatttttg tagcaattgt tggcactatt ctcattccat
     5941 taatacaaaa atatgttcct gcaatagacc atcatttaca aaataagtca gaaaagaaat
     6001 cgccttttga agcattacat attattaggg aagatagaaa tttaattttg gcacttacgt
     6061 ttagtggctt agttatgcta gggcattttt taattattcc ttttataaac ccttatatgg
     6121 aatttaataa tggcttttca aagcagttaa cacctatgat ttatttagtt ggcggcattg
     6181 cttcatttat atctgccaat atattaggta gaatttctga taagtatgga aagctaaaag
     6241 tatttagtat ctgcattttt ataagtttac cattggtaat tggtgtaaca catgtacctg
     6301 caattccatt ttggattata cttgtaatgt ttgctttttg gtttacagtt gctacaggaa
     6361 gaggtgtaac tgcacaagca atgataagta atgcagtaca agctaaacat agaggtagtt
     6421 ttatgagttt taacagtagt gttcagcaat taggtacaag tattgcttct ttagttgcag
     6481 gggtaattgt aataaaagaa aacgatggaa aactacacca ttataaatgg ttaggctatt
     6541 taagcgtagt ggttttatta gcttgcctgt ttttagggag atatattttt aaaaaagaaa
     6601 attattcaac ttctcagtta tctgaagaca atatttcgga gttgatataa acatacaatt
     6661 caccttttaa aaatcaacat ttttctactt gtaatattta gttgttatta attacattta
     6721 ttgctgaaaa aggtttacta aatgaatcaa aaaaaattta caattctttg cttaggagat
     6781 tcttatacta ttggagaaaa tgtacccatt catgacagct ttccttatca aacaatacaa
     6841 ttattaagaa agaaggggtg taacttttat gcaccagaaa tagttgctaa aactgcttgg
     6901 acaagctttg aacttataga acatttatct ggcataacac tgctggaaaa ttacgattat
     6961 gttaccctct tgattggtgt aaacaatcaa tatagaggct tagagataga agaatttaaa
     7021 aaagattttg aatggctaat aaacaaagct ttacaacttg ttgggtttaa cagtaataag
     7081 gtaattgtat tatctatacc caattggggt gttacaactt ttgcaaaaga tagagatgaa
     7141 aaaagcataa ctaaggctat agaaagttat aatttaatat gtaaacaatt agcagaaaat
     7201 aataaaacta actttattga tataactaac cattctttac ttgcaaaaga taaccaacaa
     7261 ttattagctg cagatggttt gcactattct ggtttagcta tgcaagaatg ggcggtaatg
     7321 ttagccaatg ttatcagtaa ggatatttta acagagaaaa aataagagca attatatact
     7381 tttttatatt taattcgtta ttaaaaggct aaaccttggt gtttaatgaa atttattttt
     7441 acttgcatat tatggttatt tttttactcc attcctttat cagctcaata ttatttacga
     7501 ggtgaaatta aagatgaaaa aggcaagttt ttacctaacg taaaaattag tttattttca
     7561 aaaaataatt ttagattttc ttatacaagc ggaaattctg gttcatttgg aataccaagt
     7621 acattagaaa ttgata
//
LOCUS       DAOQHP010000059         7603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5043_length_7603_cov_3.526497, whole genome shotgun sequence.
ACCESSION   DAOQHP010000059 DAOQHP010000000
VERSION     DAOQHP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_7603_cov_3.526497"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..969)
                     /locus_tag="PLY81_03145"
     CDS             complement(76..969)
                     /locus_tag="PLY81_03145"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNF29256.1"
                     /translation="MNCPKGKLIAIGGAEDKGTDLEKGEILRNNLNFFELGILKRIIE
                     ETGGLDKRIEVVTTASSIPYEVGENYLNAFGKIGCTNVGLMHIRNREDVKKEEYLERV
                     KQCNAIMFTGGNQMRLSSIFGGTTFLDIIKSRYLQEEFIIAGTSAGAMAMSNTMIYEG
                     NATKAHLKGEVKITTGLAFMENVIFDSHFEKRGRFGRLAQAVATNPSCIGIGLGEDTG
                     MMITKGNVMQAIGSGLVIIIDGHELGHCNIADIPEGNPISVENLKVHFCENGNGYLVN
                     ERKFIVEVAQGALVEKKVEVE"
     gene            1168..2043
                     /locus_tag="PLY81_03150"
     CDS             1168..2043
                     /locus_tag="PLY81_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF29257.1"
                     /translation="MQKLLFTLLFWQLHVTTVFAKADTSIVVFFDFNKYELTQNSTNI
                     LKQFCADTLVLELIEIYGHTDQIGKEKYNLLLSQKRANSVKQFLLTNFIDNNKIIVTQ
                     GKGEKELITNKTKEEFRKENRRVVIYFEKRQPIVKPAIVEKKPEEKPKKPIEKETTLT
                     EKIKDSTVKTGDNLILKNINFVGGRHVFLRESYTALKELLQAMQNIPTLEIQIEGHIC
                     CQMANEDGVDLDTGTKDLSVRRAKAVYDFLVSNGIEPSRMIYKGFARKFPITDESNEF
                     ERMTNRRVEIKIIKK"
     gene            2163..4112
                     /locus_tag="PLY81_03155"
     CDS             2163..4112
                     /locus_tag="PLY81_03155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF29258.1"
                     /translation="MQVKQFCFLVSILFCCNHIIAQPNRWQQKIKYTITVELNDTENK
                     LNGIEKIEYTNNSLDTLNKIFFHLYWNAFQPNSSMDVRSRELGKTVIRKDKDGKDVLD
                     WDGRVTNRISQLKENEIGIQKVKYVVINGKQQKLIEHETILEVVLDKPILPNSKTVMN
                     VSFEAQVPIQIRRAGRDNAEGIKFSMSQWYPKVVEYDYNGWNANPYIAREFYGVWGDY
                     DVKIKLNNKYTVAATGVLQNPNTIGKGYSTPGLKVAKPSKDVITWNFIANNVHDFVWA
                     ADPDYTHESKEIRKGLTLHLFYKSTTPANDSAWHNILWAAEKVMPYIEKRFGKYPYPQ
                     YSFIQGGDGGMEYAMATLIKGSGLGTVFHEWMHSWYQFVLANNESLYGWMDEGFTTFA
                     EDEVTEWYNKNYAWQSPWITEKAKQKIIFQNKNNDTELPLKQYNNYAAYFNLVKSPFE
                     EPLSTHADHFNTNFGYSYASYIKGSVFLTQLGYITSDSVRDKILLAYYDTWKFKHPNV
                     NDFIRVAEKVSGLELQWYKEYWVYTTKNIDYALANINLDTITNKEILTLKRIGKMPMP
                     IDVLITYKDGSSEMYYIPLNLMYGNKPNESNTKNFVLTEWKWTHPEYTFSINKNINEI
                     KSIEIDPTQRMADINRINNKILIPD"
     gene            4123..4728
                     /locus_tag="PLY81_03160"
     CDS             4123..4728
                     /locus_tag="PLY81_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNF29259.1"
                     /translation="MISNDTKNILGLQLPTDPRWANLTEISLEAILTDHAYCEQKAAT
                     TCISLIQRFSKREKLVKELAPIVTEEWGHFRLVLNELHKRNLKLGNQRKDEYVNQLIA
                     FQQKGGNEEGRLLDQLLTMALIEARSCERFKLLSENLSDKYLRNFYRRFMESEAGHYT
                     LFITLAETYIDVLKVKKRWKEWLAYEADIMKSLELRGDRIH"
     gene            complement(4777..5595)
                     /locus_tag="PLY81_03165"
     CDS             complement(4777..5595)
                     /locus_tag="PLY81_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29260.1"
                     /translation="MKKNIVVLIFSFIPALLLAQQTKNLTPRQQKKADRREKIENLIK
                     KEEEGALIFNKQTVFGIKLSTDGYGLHFEKGKFKTNKLTTLWWSELNEHKHAKEQKFA
                     TGDPNWGFTIGTPYVYGKINNFYNYKLGYGQQRLIGGKGTKNGVATSLIYGGGIAIAI
                     LKPYFLDVQDPLSLETKTIRYKDDEQLFLDQTRIIGSAGIFKGIGYSKFIPGFHVKTA
                     LRFDYGRYNEMISALEAGINFEYYTKEIEHMALNKNHKMFFNAYVALVFGKRKN"
     gene            5638..6045
                     /locus_tag="PLY81_03170"
     CDS             5638..6045
                     /locus_tag="PLY81_03170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF29261.1"
                     /translation="MTSTVVYKGDLRTELTHLQSGKVIETDAPTDNHGKGERFSPTDL
                     MATSYASCMITTMAIKAADMNLNFDGTSINVTKIMSTDAPRRIAGIVTELNFTEKFTP
                     SNEQKEQLIRIARSCPVEKSLHPDVQLTVTFNW"
     gene            complement(6056..6556)
                     /locus_tag="PLY81_03175"
     CDS             complement(6056..6556)
                     /locus_tag="PLY81_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNF29262.1"
                     /translation="MPDKKYILNKQEASLKIKRLALEVAEILISNKNEIIIIGIKKNG
                     VTIAEKVAELLKQYVQQPLTVLQLSLNKATANFIELSENIDFTNKYVLLCDDVVNSGK
                     TLLYALKPILEQYPASIQTLVLVERMHTLFPIKADFVGISLTSTLQNNIVVEIENNEI
                     TGAYLE"
     gene            6696..>7603
                     /locus_tag="PLY81_03180"
     CDS             6696..>7603
                     /locus_tag="PLY81_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HNF29263.1"
                     /translation="MQFIPQILFLIITGIAIYLFYKKASEIRRNILLGKEEDFSDNKN
                     KRWNNMILLALGQKKMFRNPIVALLHFIVYAGFIIINIEVLEIVLDGILGKHRLFAGL
                     LPMGLYQFLINSFEILALLVLTACVVFLIRRNIIKVKRFTGNDLKGWAYNDANYILFT
                     EIVLMSMFLIMNASDTLLQERNFATYAQHSTGNFIISSYIQPLINSFSDNSLVLIERV
                     CWWLHIIGIFAFLNYLPYSKHLHVLLAFPNAYYARLEPKGKMKNMPSVQNEVLYAMQP
                     ELAPTNAAPPERFGAKDVFDLSWRNL"
BASE COUNT         2786 a         1147 c         1138 g         2532 t
ORIGIN      
        1 gtgtagataa tgcaagtagt aaccaagcag ttttattaaa agagttaact ataaaaatat
       61 tttcaaatta aatagttact ccacttcaac ctttttttca actagtgctc cttgggcaac
      121 ttctactata aattttcttt catttactaa ataaccatta ccgttttcac aaaaatgtac
      181 ttttaaattt tctacactta ttgggttacc ttctggaata tctgcaatat tacaatggcc
      241 aagttcgtgt ccatcaataa taataactaa accactacct atagcctgca ttacattacc
      301 ttttgtaatc atcatacctg tatcttcacc taagccaata ccaatacaac ttgggttagt
      361 agctacagcc tgagccaatc ttccaaatct tcctcttttt tcaaaatgag aatcaaaaat
      421 tacattttcc ataaatgcca accctgtggt aatcttcact tcgcctttta aatgtgcttt
      481 ggtagcattc ccttcatata tcattgtatt gctcattgcc atagcacctg cactagtacc
      541 agcaataata aattcttctt gcagatatcg gcttttaata atatctaaaa atgtagttcc
      601 accaaaaata gaagacaacc tcatttgatt accacctgta aacattattg cattgcattg
      661 tttaactctt tccaaatact cttctttttt tacatcttct ctatttctta tatgcattaa
      721 cccaacatta gtacagccaa tttttccaaa tgcattcaaa tagttttcgc ccacctcgta
      781 aggtatagaa gatgcagtgg taacaacttc tattctttta tctaaaccac ctgtttcttc
      841 aattattctt tttaaaattc ctaattcaaa aaaattaaga ttattacgta gtatttcacc
      901 tttttccaaa tcagtgcctt tatcctctgc tccaccaatg gcaattaatt ttcctttagg
      961 acagttcatt caaaaaaaat tttagttaat agggttctaa tgatttatct ggtttttgta
     1021 taaatcaatt tttattcatt aacaaatata tagtttacag cataatatta tgtgtttaat
     1081 cttttacaca ttgtgtatat tgtaattaca atatattcaa ataagtgtag ttttattggt
     1141 atatcttgct attaaaacaa tttttaaatg caaaaattac tctttacatt acttttttgg
     1201 caactgcatg ttactacagt atttgcaaaa gcagatacta gtattgtggt atttttcgat
     1261 tttaacaagt atgaattaac ccaaaattct acaaacattt taaaacaatt ttgtgccgat
     1321 acattagttt tagaattgat agaaatttat gggcataccg accaaattgg taaggaaaaa
     1381 tataatttat tattatctca aaagagagcc aatagtgtaa aacaattttt acttacaaac
     1441 tttatagata acaataaaat aattgttaca caaggcaaag gagaaaaaga attaattacc
     1501 aataaaacca aagaagaatt tagaaaagaa aacagaagag ttgttattta ttttgaaaaa
     1561 aggcagccca tagttaagcc tgcaatagtg gagaaaaagc ctgaagaaaa acccaaaaag
     1621 ccaatagaaa aggaaaccac tttaacagaa aaaataaaag acagtactgt aaaaacaggc
     1681 gataacttaa ttttaaaaaa catcaatttt gtaggtggtc gtcatgtttt tttaagagag
     1741 tcttatactg ctttaaaaga attactacaa gccatgcaaa atataccaac cttagagata
     1801 caaattgaag ggcatatttg ttgtcaaatg gcaaatgaag atggtgtaga tttagataca
     1861 ggcaccaaag atttatctgt aagacgtgca aaagctgttt acgatttttt agttagcaat
     1921 ggaatagaac ccagtagaat gatttataaa ggctttgcca gaaaatttcc tattacagat
     1981 gaaagcaatg agtttgaaag aatgaccaac agaagggtgg aaataaaaat tattaaaaaa
     2041 tagcctctat cttattctta agccattttc ttaccacaat tatataagtt ttaagaaaat
     2101 attgtagtaa tatttgtttc tttggcattg tacatgacat ggaagtttta aatttatttt
     2161 ttatgcaagt aaagcagttt tgttttttag taagtattct tttttgttgt aatcatatta
     2221 ttgctcaacc caatcgttgg caacaaaaaa taaaatatac tattactgta gagttgaacg
     2281 atacagaaaa taaacttaat ggtatcgaaa aaattgaata caccaacaac tcactagata
     2341 cgttaaacaa aatatttttt catttatatt ggaatgcttt tcaacctaac agcagtatgg
     2401 atgtaagaag cagagaacta ggtaaaacag ttattagaaa agataaagat ggaaaagatg
     2461 tgttagactg ggatggtaga gtaaccaata gaattagcca gttaaaagaa aatgaaatag
     2521 gaattcaaaa agtaaaatat gttgtaatta acggaaaaca acaaaagctt atagaacatg
     2581 aaacaatttt agaagtggta ttagataaac ccattttacc caactctaaa acagtaatga
     2641 atgtaagctt tgaagcacaa gtgcccatac aaataagaag agcaggaaga gataatgcag
     2701 aaggaataaa atttagcatg agccaatggt atcctaaagt tgtagaatac gattataatg
     2761 gctggaatgc caacccttat attgccagag agttttatgg cgtttgggga gattatgatg
     2821 taaaaattaa actaaataat aaatatacag ttgcagctac aggtgtacta caaaatccaa
     2881 atacaattgg taaaggctat agtacaccag gtttaaaagt tgctaaacca agtaaagatg
     2941 taattacatg gaattttatt gcaaataatg ttcatgattt tgtttgggct gccgacccag
     3001 attatacaca tgaaagtaaa gaaataagaa aaggccttac tctgcatctc ttttataaat
     3061 caacaacccc agccaacgat agtgcctggc ataatatttt atgggcagca gaaaaggtaa
     3121 tgccctacat agaaaaaaga tttggcaagt acccatatcc acaatactcc tttattcaag
     3181 gtggtgatgg tggaatggaa tatgctatgg ctacattaat aaaaggaagt ggtttaggaa
     3241 cagtttttca tgaatggatg catagttggt accaatttgt tttagcaaac aacgaaagcc
     3301 tttatggctg gatggacgaa gggtttacca cttttgctga agatgaagta acggaatggt
     3361 ataataaaaa ctatgcatgg caaagcccat ggataacaga aaaagcaaaa caaaaaataa
     3421 tttttcaaaa taaaaataac gataccgaac tgccattaaa gcaatataac aattatgctg
     3481 cttattttaa tttggtgaaa agccctttcg aagaaccatt atcaacccat gccgatcatt
     3541 ttaacaccaa ctttggatac tcttatgctt catatataaa aggctcagtg ttcttaacac
     3601 aattgggtta tatcaccagc gattcagtga gagataaaat tttattagcc tattatgaca
     3661 cctggaagtt taaacatcct aatgtaaacg attttattag agttgcagaa aaagtaagtg
     3721 ggctagaatt acaatggtac aaagagtact gggtatatac aactaaaaat atagattatg
     3781 ctttggctaa tattaaccta gatactatta ctaacaaaga aatattaact ttaaaaagaa
     3841 ttggtaaaat gcctatgcca attgatgttt taataaccta taaagatggt agtagcgaaa
     3901 tgtattatat acctttaaac cttatgtatg gcaacaaacc aaacgaaagt aataccaaaa
     3961 actttgtatt aacagagtgg aagtggacac atcctgaata tacttttagc attaataaaa
     4021 atatcaatga aataaaatct attgaaattg accctactca aagaatggca gacataaaca
     4081 ggataaacaa taaaatactt attccggatt aagaaattaa acatgatttc aaacgataca
     4141 aaaaatatat tagggttaca attaccaacc gatccacgtt gggctaattt aacagaaatt
     4201 agcttagaag ctattttaac cgatcatgct tactgcgagc aaaaagcagc tacaacatgt
     4261 atttcattaa ttcaaagatt tagtaaaaga gaaaaattag taaaagaatt agcaccaata
     4321 gttacagaag aatggggaca ttttagactt gttcttaacg agctacataa aagaaattta
     4381 aaactaggaa atcaacgaaa agatgaatat gtaaaccaat taattgcgtt tcagcaaaaa
     4441 ggtggtaatg aagaaggaag gctattagac caattactta ctatggcttt aattgaagcc
     4501 agaagttgtg aaagatttaa attattaagc gaaaatttaa gcgataagta tttaagaaat
     4561 ttttacaggc gttttatgga aagcgaagca ggccattata ctttgtttat aacacttgca
     4621 gaaacttaca ttgatgtact caaagtaaaa aaacgttgga aggagtggct tgcttacgaa
     4681 gcagatatta tgaaatcatt agagctacgg ggcgacagaa tacattaatt ttttaagctt
     4741 gcatgttttt cataaataac agaaaagaat tggtacttag ttctttcttt taccaaatac
     4801 caaggctaca taagcattaa aaaacatttt atggttttta ttaagagcca tgtgttcaat
     4861 ttctttagta taatactcaa agtttatacc tgcttctaaa gcagatatca tttcgttata
     4921 tctgccataa tcaaacctta aagcagtttt tacatgaaag cctggaataa atttcgaata
     4981 accaattcct ttaaaaattc ctgcagagcc tattattctg gtttgatcta aaaataattg
     5041 ttcatcatct ttatacctta tagtttttgt ttctaaacta agtgggtctt gcacatctaa
     5101 aaagtaaggc tttaaaattg caatggctat tcctcctcca taaattaacg aagttgctac
     5161 tccattttta gtgcctttgc ctccaattaa tctctgctga ccatagccta atttatagtt
     5221 ataaaaatta tttattttac catatacata aggtgtacca attgtaaatc cccaattagg
     5281 atcacctgtg gcaaattttt gttctttagc atgtttatgc tcatttaatt ccgaccacca
     5341 aagggtagtt aacttatttg ttttaaactt tcctttttca aaatgcagac cataaccatc
     5401 tgtacttagt ttaatgccaa atacagtttg cttattaaaa atcaatgctc cttcttcttc
     5461 tttcttaatt aggttttcta ttttctctct tctatctgct ttcttttgct ggcgtggagt
     5521 aaggtttttt gtttgctgtg caagtaataa agcaggtata aaactaaata tcagtactac
     5581 aatattcttt ttcatgaata aattattggt tgtaaattta gggcaaaatt tagtattatg
     5641 acaagtacag ttgtttacaa aggagattta agaacagaat taacacactt acaaagtgga
     5701 aaagtaattg aaacagatgc acctactgat aatcatggaa aaggagaacg ttttagccca
     5761 acagatttaa tggctacaag ctatgcaagt tgtatgatta ctacaatggc aataaaagct
     5821 gccgatatga acttaaattt tgatggcacc agtattaatg taaccaaaat tatgagtacc
     5881 gatgcaccta gaagaattgc aggaattgta actgaattaa attttaccga aaaatttaca
     5941 ccaagcaacg aacaaaaaga acaattaata agaattgcaa gaagttgccc tgttgaaaaa
     6001 agcttacatc cagatgtaca attaacagta acttttaatt ggtaataaat actttttatt
     6061 caagataggc tccagtaatt tcattgtttt ctatttctac aacaatatta ttttgtagtg
     6121 tagaggtaag gctaatacct acaaaatctg ctttaattgg aaatagagta tgcatacgtt
     6181 ctaccaaaac caatgtttga atacttgcag gatattgctc taaaattggc tttaaagcat
     6241 acaacaatgt tttgccacta tttacaacat catcgcataa taaaacatat ttattagtaa
     6301 aatcaatatt ttctgaaagc tctataaaat ttgcagtggc tttatttaaa cttaactgca
     6361 acacagtaag aggttgctgt acatactgtt ttaacaactc agctactttt tcggctatgg
     6421 ttactccatt tttcttaata ccaataataa taatttcatt tttattactt attaatattt
     6481 cggcaacttc taaagccaat cttttaattt ttaaagaggc ttcctgctta tttaaaatat
     6541 attttttatc tggcattact taatttttct acctcaaaat aaatacaaat aaatgaatgt
     6601 acaagtaatg ttttaataca ctgaattgta ttgtattaaa taataattta caacttatct
     6661 ttgaaaagct attttaatat tttaatttaa tttatatgca atttatacca caaattttat
     6721 ttttaattat aacaggtatt gcaatttatt tattttataa aaaagcaagt gaaatacgcc
     6781 gaaacatttt attaggtaaa gaagaagatt ttagcgacaa taaaaataaa cgatggaaca
     6841 atatgattct attggctctt ggtcaaaaaa aaatgtttag aaacccaatc gttgctcttt
     6901 tgcattttat tgtatatgct ggttttataa ttattaatat tgaagtttta gaaatagttt
     6961 tagatggcat tttaggaaaa cataggttgt ttgccggctt attgcctatg ggtttatacc
     7021 agtttttaat taatagtttt gaaattttag ccttattggt attaacagct tgtgttgtat
     7081 ttcttatacg tagaaatatt attaaagtaa aacgttttac tggcaacgac ttaaaaggtt
     7141 gggcttataa cgatgccaat tatatcttat ttactgagat agttttaatg agtatgtttt
     7201 taataatgaa tgccagtgat actcttcttc aagaaagaaa ttttgcaacc tatgctcaac
     7261 attccacagg caattttatt atttcttctt atatacaacc tcttataaat agtttttcag
     7321 ataacagttt agttttaata gaaagagtat gttggtggtt acatattatt ggcatatttg
     7381 ctttcttaaa ctatttacca tatagtaaac atttgcatgt tttgttagct tttcctaatg
     7441 cctattatgc aaggttagag cctaaaggaa aaatgaaaaa tatgcccagc gtacaaaatg
     7501 aagttttgta tgcaatgcag ccagagcttg cacctacaaa tgcagcacct ccagaaagat
     7561 ttggtgccaa agatgttttt gatttaagct ggagaaattt att
//
LOCUS       DAOQHP010000060         7541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5103_length_7541_cov_3.639060, whole genome shotgun sequence.
ACCESSION   DAOQHP010000060 DAOQHP010000000
VERSION     DAOQHP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5103_length_7541_cov_3.639060"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..105
                     /locus_tag="PLY81_03185"
     CDS             1..105
                     /locus_tag="PLY81_03185"
                     /inference="COORDINATES: protein motif:HMM:NF022462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2847 family protein"
                     /protein_id="HNF29264.1"
                     /translation="MKHESPQILVIKNGICIYNESHTAINMEDIIAIA"
     gene            complement(109..1143)
                     /gene="mltG"
                     /locus_tag="PLY81_03190"
     CDS             complement(109..1143)
                     /gene="mltG"
                     /locus_tag="PLY81_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNF29265.1"
                     /translation="MKKIFLYLVLVSALLALIIGYFAFTSTTKFTSKSTTFIIDNINK
                     QQISNLLVEKNIISNKTVFLWIGNAVGIWEKIKPGKYEVKKGESIIGIIKMLKNGRLA
                     ESKLVINKLRVKEDFAKLVSKNFSTDSVTVMQFLTSTDSLKMYAVDTNTVFTLFIPDT
                     YAFYWNTPLRKIMNKLNEAKNNFWNNNNRLEKSKAIGLSPENIYILASIVEEETNYKE
                     DRAKIASVYINRLHKNMPLQACPTIKYAMKDFALTRIYEKYLVNPSPYNTYRVKGLPP
                     GPICTPSPKCIDLVLNAPQTDYLFFVANANFDGYHHFSTNYAEHNKYAIEYQKALDLY
                     TAKKKEKNSK"
     gene            complement(1192..1965)
                     /locus_tag="PLY81_03195"
     CDS             complement(1192..1965)
                     /locus_tag="PLY81_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF29266.1"
                     /translation="MIEIKNITKSFSGRTIISNINATFETGKCNLIIGASGSGKSVLL
                     KVMVNLFTPDNGEALYDGISITALEQERRKELRQQIGMLFQGSALFDSMTVEQNVLFP
                     LDMFTKLSQADKKKRVNEVLERVNLVDANKKYPSEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPQTSLLIDKLIKEITTEYNMTTIVVTHDMNSVMEIGDYIMYLHKGSKQW
                     EGSNKDIIYSNDELLNKFIFASEFLQDAKQMRMLEENNK"
     gene            2107..2691
                     /locus_tag="PLY81_03200"
     CDS             2107..2691
                     /locus_tag="PLY81_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNF29267.1"
                     /translation="MSNKLEILSEIIANRRTTKPQLMNGKLIEEKTILKLLELANWAP
                     THANTEPWRFIVYTPETKTKFCKAHAALYKLHTDKDKFLLATFQKLEQMGNLASHIII
                     AYAKSGSNIKIPIVEEVAATSAAIQNFILAAEALNIATYWGTGGMIHQPVFKSFLNLN
                     TDDVVLGTIYLGYTNEKKEGKRITTIHEKINWVR"
     gene            complement(2711..3289)
                     /locus_tag="PLY81_03205"
     CDS             complement(2711..3289)
                     /locus_tag="PLY81_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HNF29268.1"
                     /translation="MSLEKNLQTRSNNECELCTAKKSLSIYNLPPDAEQLVEKSILIC
                     PTCLSQVEKKAPLDKEHWQCLLTSMWSEVDAVKVVSWRMLNRLREETWASDALEMLYF
                     EDDILNWAKATGDHENSSDIEFHTDCNGAILQNGDTVVLIKSLDVKGSTVNARLGTVV
                     KNIRLVADNTSQIEGKIEGQTIVILTKYLRKG"
     gene            complement(3331..3783)
                     /locus_tag="PLY81_03210"
     CDS             complement(3331..3783)
                     /locus_tag="PLY81_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF29269.1"
                     /translation="MQNIFNLTDTNELIKRINNLTPNTQPQWGKMNVVQMLAHCNVTY
                     EMIFENNFPKPTGIKKLILKYIIKNIVVGEKPYKKGIPTAPQFIITDSREFEVEKNRL
                     IHYINKVQQLGENFFDGKESHSFGKLTKQQWNNMLGKHLNHHLAQFGV"
     gene            complement(3880..>7541)
                     /locus_tag="PLY81_03215"
     CDS             complement(3880..>7541)
                     /locus_tag="PLY81_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29270.1"
                     /translation="IGKTYTFYFYATNVNNAGTGSSPEVSVRMDGTPGRPDGTLYIGP
                     TELLESETNNSQPLKGWKRFSLTGVATATSHRVTITDSETGSSRDELGITAIGITACE
                     TICEAPQAYSVGGANTIINSYYPATANAPAGSTRIQVGDKRTEGAGDNIQAGDLLLVI
                     QMQGSEINSTNTNNYGANNGTYRGYTSSVAGTYEYVYAASPVVAGVVYLATPLKKSYI
                     NANATGTAGQYRFQVVRVPQYTSLNIPSGASITNAEWNGASGGIIAANVSGTMTLNSG
                     VAIDASALGFRGGGGRMLTGGTGANTDTRTLSSNTTNGSKGEGIAGTPKYTRSIADVL
                     VDNGAEGYPNGSYAQGAPGNAGGGATDGRVSANDQNTGGGGGGNGGIGGRGGRAWSNP
                     AQYGGYGGGKFNEAASNRLVLGGGGGAGTTNNGTAEGMGYSVNYQNGFYSSGGSGGGM
                     IFLRVGAVSGSGAINANGADGLAVENDGGGGGGAGGSVYLFATNTAGLNNITVNAKGG
                     AGGNAWIYTADNGIPNDGDPEHGPGGGGSGGIIYSNGALNAASSVVGGNPGVTTTSNL
                     PYGASKGDDGIKLTSAVDAMINVVKVFCDIDDDDDGIADVIENANGLVDAFLDADGDG
                     IPNTYDPMPGTGVAEWGDTNSDGISDKYDNDNDGIINELDLDSDNDGVTDVIESYGVD
                     TNGDGIIDNYTDGNGDGLSDNAANTNAINGLGGIDTDGDGIPNYLDLDSDNDGIPDIV
                     EVGALDNNNNGKVDTFIDTNFNGINDSLEGVSNALVLSGSDTNNNGVADSWPNKNADR
                     TGRPNLYDLDSDGDGITDAEESGLIGRTGTNGTVASSAGIITGTYSNGWANTVQGLAS
                     LNLPNTDARGSANYLDIDSDDDGITDNVEAQSTSSYVVPTDTDSDNDGIADVYEVPAQ
                     IGTYGGNGLTPYNKDGDGNPDYKDTDTDNDGALDYIEGTGSSLYGVAYNTLNFTDTDG
                     DGLVDQWDIANISSLTAGNLYRNVTHSDMGPGGNLDGPAPSGSSARLPKHTWGPSTDR
                     DWRSNIILPLNIISFKVNYNAPNAVINWEVQNELQTENYDVEFSINGTEFTKIFTVQA
                     KNIGNANYTQWHNLDNYTQNVFYYRIKQTDKDGKIYYTNIASIKVNRDSKITVAPNPF
                     RNHFSVNYNSVSNEKVTISLISNDGKIISTKQVDVVKGINQIMFDNLQYLQHGLYFLR
                     VQTLKNITTLKLLKE"
BASE COUNT         2329 a         1473 c         1035 g         2704 t
ORIGIN      
        1 attaaacatg aatctcctca aattcttgtt ataaaaaatg gtatatgtat ttataacgaa
       61 agccatactg ctataaatat ggaagatatt attgcaattg cttaaaactt atttagaatt
      121 tttttctttc ttttttgcag tgtataaatc taacgccttt tggtattcaa ttgcatattt
      181 attatgctcg gcatagttag tagaaaagtg atgataacca tcgaaatttg cattggctac
      241 aaaaaataaa taatctgttt gaggggcatt taaaactaaa tcaatacact taggagaagg
      301 agtacaaatt gggcctggag gtaaaccttt taccctataa gtgttatacg gagaagggtt
      361 taccaaatat ttttcataaa tacgagttaa agcaaaatct ttcatagcat atttgatagt
      421 aggacaggct tgcaatggca tatttttatg tagcctgttt atatatacgc tagcaatttt
      481 ggctctatct tctttataat ttgtttcttc ttctactata gatgctaata tataaatgtt
      541 ttctggcgat aagccaattg ctttagattt ttctaatcgg ttattattat tccagaaatt
      601 atttttagct tcattaagtt tattcattat ttttctaagt ggcgtattcc aataaaacgc
      661 atacgtatct ggtataaata aggtaaaaac tgtattggta tccactgcat acatttttaa
      721 agaatcggtt gaggttaaaa actgcatgac agttacagaa tcggtagaaa aatttttgct
      781 tactagtttt gcaaaatctt cttttactct aagtttattg attactaatt tactttcggc
      841 caatcttcca tttttaagca ttttaataat gccaataata ctttcgcctt ttttcacctc
      901 gtattttcct ggcttaatct tttcccaaat tcctacagca tttccaatcc ataaaaacac
      961 agttttattg ctaataatat ttttctcaac aagtaaatta cttatttgtt gtttgttaat
     1021 attatcaatt ataaatgttg tcgatttact agtaaacttt gtagtagaag taaaagcaaa
     1081 atagccaata attaaggcaa gtagagcact aactagaacc aagtacaaaa atattttttt
     1141 cattttaata aaaaatgttt agaaaattat tgccaagaat aattgtaata attatttatt
     1201 attttcttca agcatacgca tttgtttagc atcttgtaaa aactccgaag caaaaataaa
     1261 tttattcaat aattcatcat tgctgtaaat aatatcttta ttgcttccct cccattgctt
     1321 actacctttg tgtaagtaca taatgtaatc tccaatttcc atcacactat tcatatcatg
     1381 tgtaacaaca atggtagtca tattatattc ggtagtaatt tcttttatta atttatcaat
     1441 taataaagat gtttgagggt ctaaaccact attaggttca tcgcaaaaaa gatatttagg
     1501 gtttaacaca attgctctag caatacctac tctttttttc attcctccgc taatctcaga
     1561 aggatatttt ttatttgcat ccactaaatt aactctttct aaaacttcat ttacccgttt
     1621 ctttttatcg gcttgagata gtttagtaaa catatctaga ggaaataaaa cattttgttc
     1681 aaccgtcata ctatcaaata aagcactgcc ttgaaacagc attcctattt gctgtcttag
     1741 ttctttcctt ctttcttgct ctaatgcagt aatgcttata ccatcgtata atgcctctcc
     1801 attatctggt gtaaaaagat taaccattac ttttagtagt acacttttac cactaccact
     1861 agccccaata attaaattgc attttccggt ttcaaaagtt gcattgatat tgctaataat
     1921 ggttctgcct gaaaatgatt tggtaatatt tttaatttct atcataacaa atgtttgtaa
     1981 agttatgcta atgcaagcaa attattggtg tacaatattt ctattatagt tgcttattga
     2041 ttaaatttta acaaccaatg catgtatttt tgaggtgtaa acaatttaat taaatacaat
     2101 tgaataatga gcaacaaatt ggaaatttta agtgaaatta ttgctaacag aagaaccact
     2161 aaacctcaat taatgaatgg caaattaatt gaagaaaaaa ccattttaaa attactggaa
     2221 cttgccaact gggctcctac acatgctaat acagaacctt ggaggtttat tgtttatacg
     2281 ccagaaacta aaacaaagtt ttgtaaagca catgcagcat tatataaatt acacacggat
     2341 aaagacaaat ttttgctagc aacttttcaa aaacttgaac aaatgggcaa ccttgcctct
     2401 cacattatta ttgcctatgc caaaagtggt agcaatatta aaataccaat tgtagaagag
     2461 gttgcagcca ctagtgcagc tatacaaaat tttatactgg ctgcagaagc tttaaatatt
     2521 gctacctatt ggggcactgg gggcatgata caccaaccag tttttaaaag cttcttaaat
     2581 ttaaatacag acgatgttgt tttaggtact atttatttgg gttataccaa cgaaaaaaaa
     2641 gaaggaaaaa ggattactac cattcatgaa aaaataaact gggtacgata aagtttaagg
     2701 aatacaaagt ttagcctttg cgtaaatact tggttaatat cacaattgtt tgtccttcta
     2761 ttttaccttc aatttggctg gtattatctg ctacaagcct aatatttttt acaacagttc
     2821 ctaaccttgc atttactgtg gagcctttta catctaatga ttttattaaa actacagtat
     2881 caccgttttg caaaatagca ccattgcaat ctgtatgaaa ctcaatgtca gaggagtttt
     2941 catggtctcc agttgcttta gcccaattta aaatatcgtc ttcaaaatac aacatctcta
     3001 atgcatcaga agcccaagtc tcttctctca atctgtttaa cattctccac gataccactt
     3061 taactgcatc tacttcgctc cacatgcttg ttaataaaca ttgccagtgt tctttatcaa
     3121 gaggtgcttt tttctctact tgacttaagc atgtagggca aattaaaatg cttttttcaa
     3181 ctaattgttc agcatctggc ggtaaattat agatagataa acttttttta gctgtacata
     3241 actcacattc attgttactt ctggtttgta ggtttttttc taaactcatt atctcttatt
     3301 ttaaagtttg caaaataaaa attaaacctt ttatacacca aactgtgcta aatggtggtt
     3361 taagtgtttg cccagcatgt tattccattg ttgcttagtt aatttaccaa aggagtgtga
     3421 ttcttttccg tcaaaaaaat tttcgcctaa ttgttgtact ttattaatgt aatgtatcaa
     3481 tctatttttt tcaacctcaa attctctact atctgttatt ataaattgcg gtgctgttgg
     3541 aattcctttt ttataaggct tttctcccac tacaatattt tttattatgt attttaaaat
     3601 taatttttta attccggttg gtttagggaa attattttca aaaatcattt cataagtaac
     3661 attgcaatgt gctaacattt gtaccacatt cattttaccc cattgtggct gtgtatttgg
     3721 tgttaaatta tttattcgtt ttattaactc gttggtatct gtaaggttga aaatattctg
     3781 catttgttaa ctcttttaaa aaaattttgg cgaaagtaaa taaaatagcc tcattaaaaa
     3841 tttaaaatga ggctaaattg aaattttatg tacttacaac tattctttta gaagtttaag
     3901 tgtagtaata ttttttaaag tttgaactct caggaaatat aaaccatgtt gtaaatattg
     3961 taagttatca aacataattt gattaatgcc tttaacaaca tctacttgtt ttgttgaaat
     4021 aattttacca tcattactta ttaaactgat tgtaactttc tcgttgctta cactattata
     4081 atttacacta aaatggtttc taaatggatt aggagcaact gtaattttgc tatctctatt
     4141 cacttttata gaagcaatgt tagtataata tatttttcca tctttatccg tttgtttaat
     4201 tctataataa aaaacatttt gagtgtaatt atctaaatta tgccattggg tgtaattagc
     4261 attgcctatg ttttttgcct gcactgtaaa tattttggta aactctgtac catttatgct
     4321 aaattcaaca tcataatttt cagtttgtag ctcgttctga acttcccaat taataacagc
     4381 attaggagca ttatagttta ccttaaatga aatgatattt aagggaagaa taatgttgct
     4441 acgccaatct ctatctgtag atggacccca tgtatgtttc ggtaatcttg cagatgaacc
     4501 agaaggagct ggaccatcca gattaccacc tggacccata tcgctatgtg ttacatttct
     4561 gtaaagatta cctgctgtta atgaactgat atttgcaata tcccattgat ccactaaccc
     4621 atcaccatcg gtatctgtaa aatttaaagt gttgtatgca acaccatata atgatgatcc
     4681 tgtaccttct atataatcta aagcaccatc attatcagta tccgtatctt tataatctgg
     4741 gttaccatct ccatctttat tataaggtgt tagcccatta cctccataag ttccaatttg
     4801 agcaggtacc tcgtaaacat ctgcaatacc atcgttatca gaatcagtat ctgtaggtac
     4861 aacataacta cttgtagatt gagcctctac attgtcagtt attccatcgt catcgctatc
     4921 aatatctaaa taatttgcag agccacgagc atcggtattg ggtaaattta gtgaagctaa
     4981 accttgcact gtatttgccc atccattact ataagttcct gtaattatac ctgcactgct
     5041 agcaacagta ccattagtac ctgttcttcc tattaaacca gattcttctg catctgttat
     5101 tccatcgcca tcactatcta aatcatacaa attaggtctt ccagttctat ctgcattttt
     5161 atttggccaa ctatcggcaa caccattatt attggtatct gagccagata acaccaaagc
     5221 attagacact ccttctaaac tatcatttat tccattaaaa ttcgtatcaa taaaagtatc
     5281 taccttgcca ttattgttat tatccaaagc tcctacttca acaatatctg gaataccatc
     5341 attatcgcta tctaaatcaa ggtaattagg tattccatca ccatcagtat caattccacc
     5401 caaaccattt attgcattag tattagcagc attatcgctt agcccatctc cattgccatc
     5461 tgtataatta tctataattc catctccatt agtatccaca ccatagcttt ctattacatc
     5521 tgttactcca tcattatcgc tatctaaatc taattcatta ataataccgt cattatcatt
     5581 atcgtattta tcgcttatac catctgagtt agtgtcgccc cattcggcaa ctcctgtacc
     5641 aggcattgga tcataggtat ttggaattcc gtcgccatct gcatctaaga atgcatctac
     5701 aagaccattt gcgttttcaa ttacatcagc aatgccatcg tcatcatcat caatatcaca
     5761 aaatactttt acaacattaa tcattgcatc tactgcagaa gtaagtttaa ttccatcatc
     5821 acctttagaa gctccataag gtaaattaga agttgtagtt actccagggt taccaccaac
     5881 aactgagctt gcagcgttta aggctccatt agagtaaatt attccaccac ttcctcctcc
     5941 accaggtcca tgttctgggt ctccatcatt tggtatgcca ttatctgccg tataaatcca
     6001 agcattacct ccagctccac ctttagcatt aactgtaata ttgttcaagc cagcggtatt
     6061 ggtagcaaat aaatacacag aacctcctgc ccctcctcct cctcctccat cattttctac
     6121 agctaaacca tcagcaccat ttgcattaat tgccccagaa ccacttactg caccaactct
     6181 aagaaagatc attcctccgc cagaacctcc actactataa aaaccatttt gataatttac
     6241 actataaccc ataccctctg cagtgccatt attagttgtt cctgctcctc cgcctccacc
     6301 taaaactaat ctgttggatg ctgcctcatt aaattttcca ccaccataac caccatattg
     6361 tgcagggtta gaccaagctc tgccaccacg tcctccaata cctccatttc ctcctcctcc
     6421 tcctccagta ttttgatcat tagcagaaac tctgccatct gtagcaccac cacctgcatt
     6481 gcctggtgca ccttgtgcat agctaccatt tggataacct tctgcaccat tatcaaccaa
     6541 tacatctgct atagaacgag tatattttgg agttcctgca ataccctctc cttttgaacc
     6601 attggtagta tttgatgaaa gtgtacgcgt atctgtatta gctccagttc ctcccgttaa
     6661 cattctacct ccccctcctc taaatcctaa tgcagaagca tctattgcaa caccactatt
     6721 taatgtcatt gttcctgata catttgcagc tattattcct cctgatgctc cattccattc
     6781 agcattagta atagatgcac cacttgggat atttaaagat gtatattgtg gtactctaac
     6841 tacttgaaaa cgatattgtc ctgcagtacc agtagcatta gcatttatgt aagacttttt
     6901 taatggcgta gctaaataaa ctacaccagc aactacaggg cttgctgcat aaacatattc
     6961 ataagttcct gcaacagaag atgtataacc tctataggtg ccattgtttg caccataatt
     7021 attagtatta gttgagttta tttctgatcc ttgcatttga attaccaata ataaatcacc
     7081 tgcttgaata ttatctcctg caccttctgt tctcttatct cctacttgta ttcttgtaga
     7141 acctgcaggt gcattggcag ttgctggata ataagaatta attatagtat ttgctcctcc
     7201 tacagaataa gcttgtggag cttcacatat tgtttcacaa gcagtaattc ctattgcagt
     7261 aattcctaat tcatctctag atgaaccagt ttcagaatca gtaatagtta ctctgtggct
     7321 tgtagctgtt gcaacaccag ttaaggaaaa tcttttccac ccttttaatg gttgactatt
     7381 gttcgtttca gattccaata actctgttgg gccaatatat aaagtaccat ctggtcttcc
     7441 aggtgttcca tccattctta cgcttacttc cggagaacta ccagttcctg cattgtttac
     7501 attagttgca taaaaataaa aggtgtaagt tttgcctata g
//
LOCUS       DAOQHP010000061         7503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5146_length_7503_cov_3.665682, whole genome shotgun sequence.
ACCESSION   DAOQHP010000061 DAOQHP010000000
VERSION     DAOQHP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5146_length_7503_cov_3.665682"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4363)
                     /locus_tag="PLY81_03220"
     CDS             complement(<1..4363)
                     /locus_tag="PLY81_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNF29271.1"
                     /translation="MKKFLFFFCFLLLAIGSTIAQPMWSIATCSNLTSTTYGPMYSTS
                     TANATNRTAIIYPATQLVGVANTTLTSMFIKRATASGSMLGTPNFKIYLLETSSTEWG
                     AGSLDWATATGPATLVYDGNPVSAVGSSAGFKEFSLTTTFAYGTQNLAVFFEYTNSTT
                     SNSISWDYEYGIGCSGNNNNTGKYTNNTTGVLPATLASSNYRRPVIGFNYSLPPCTVP
                     PTAGIAISNIDTACSGEDFILNLSGNSFGDGQTYQWQYSTTGTAPWTDIGTADIEQRD
                     TLNQLTTTYYRCAVTCSGNTAYSDSVKIFTPTLVSGTFTIDQTLPPSGSNFTSFADAI
                     NSIKCGISGPVVFNVVAGSGPYTITSPISIPDVYGASPSNTITINGNNQSIVYNASVS
                     ADRSAIILNGADFVSLNNLSIDVSAGTYGWGITLINAADSNYIGSCNITSSTSSTSTN
                     YAGIVITGSLTSATSIGNNGNGNLIENNNIIGGYYGISTYGESTTLCAGNKLVNNSIQ
                     DFNYYGIQAAYQADAEIKNNDIQRPNRTTFSTTNYGMNFISVSTGLNVSGNKIHHLFS
                     ALPVNTITTYAIYVSADGTVTNPNKFYNNLIYSIEGDGTHYGVFNSGGAYMQAYHNTI
                     ALDYVAATAGTTAGFWQTTTADGIEFKNNIVYITRGGTGAKYCIYKATPATPLVSNNN
                     AFYLGSAGSGAQSIGYETSARASLSDWQTATSQDLNSLVADPVFTDPISGDYSFTSSI
                     IDNLGSPLGITTDIIGNSRSGSTPDVGAYEYVAPACIAPPTAGSATSSSPNVCNGETF
                     SLNLTGNSSGTGQTYQWQYSTTGSAPWTDIDIADGSPLKSVSQITSTYYRCAVTCSGN
                     TTYSTSVQVVSPAMVSGTFTINKLLPTSGTNFASFAEAIDSISCGINGPVIFNVVSGS
                     GPYALPSQIIINSIYGTSTSNTITINGNGETVTYNASVSETRTALLLNGIDHLIIDSL
                     TIDVSAGTYGYGIQFMNQSDSNIVRKSTILNNTTATTSNYAGIVYSGSLTSATIADDN
                     GNGNLLENNRIVGGYYGITNIGAASSLNSNNTIRNNSVEDFYYYGIYFSNQNNGIIEN
                     NNIHRPLRTNSSSCYGIYSTTGNKGLFINSNKIHNTFAGQVSSTSSLYGIYISSDAPA
                     ASPNLIANNLIYANEGNGTHYGLYSIGSDTALFYHNTVSLDYTPATAGATYGIYMSST
                     ASSGVQYKNNIISITRGGSGVKYGFYDLSSNLYASNYNNFYLNSLGAGAQYVGYKSST
                     AQATLLDWQTASSNDMNSYVLNPMFYNPSPIGSIYLPTNGSLQNSGTPLSIPTDINGD
                     TRNLSTPDIGAYEMPAFYHYRSQATGNWGDLSTWESSTDSSSWAAATTIPSYYSLSAV
                     VSNGHTVTINSSATIDKTFVNPTGILAVNDALTVYLNGLTFRSSSSGTGALGQSTGSI
                     TGNVVVERFIPAKATRKW"
     gene            complement(4869..5267)
                     /locus_tag="PLY81_03225"
     CDS             complement(4869..5267)
                     /locus_tag="PLY81_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29272.1"
                     /translation="MIKFNELQTGDFVMVEYEGKMWEGEVTNLNHDEKQVEVTTEVQP
                     FWYAPESLHAVPLNDQQLMNFNFTKQVNEDGSIKYMKEAFRLVIPSKDDFSTIEMWYR
                     EDRRHHPDVHFMHQLQNHYYSMTKVHLTKS"
     gene            complement(5340..5804)
                     /locus_tag="PLY81_03230"
     CDS             complement(5340..5804)
                     /locus_tag="PLY81_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF29273.1"
                     /translation="MSQHSLDTLDFNILSCLQQDGRMSFTVMAEKLNVSIATVRTRVN
                     KLIEDGTINIIGRVNPEKVGFHAYAHIAVYVRPATLKEKVANKISKYKEVSFLALTSG
                     DYDLEVDVMCKNNDFLVEFVNKISTIEGVHQTKTTIYFKVYKYAQPDLDLLR"
     gene            5966..7423
                     /locus_tag="PLY81_03235"
     CDS             5966..7423
                     /locus_tag="PLY81_03235"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNF29274.1"
                     /translation="MKYPAIQNFINGKFVSASTTKTMDVISPLNGSHLSTVPMSAYND
                     LDNAVRAAKAAFPAWSKTPIKERVQVFFRYKTLLEKHLKELAELCTEENGKTYAESVA
                     EIEKCVELTEFATSLPQLIQGEILEVSKGVECRTEHAPLGVVASIVPFNFPSMVPNWT
                     IPNAIALGNCMIIKPSEKVPLSCGRLAEILKEAGLPDGVFNVVHGDVEIVEAICDHPG
                     IEAVSFVGSTKVAKIVYQRATQNLKRCLALGGAKNHLLVLPDAIPDMTAQNVAASMSG
                     CAGQRCMAASAMVAVGSVQHIIDKICEEAKKIIPGKNLGAVINKESQLRIEKYITEAE
                     QQGAKILVDGRNTKVEGQEYGTYVGPTVIDYVTPEMSVAKEEIFGPVISIMRTKTVDE
                     ALAIENANPYGNAASVFTQNGGMARYIIDKASAGMIGVNVGVPVPREPFSFGGWNESK
                     FGVGDITGKSSIEFWTKLKKSTTKWNAEAGINWMS"
BASE COUNT         2605 a         1214 c         1243 g         2441 t
ORIGIN      
        1 tccattttct ggtagctttt gctggtataa agcgttctac aactacatta ccggtgatag
       61 agcctgttga ctgacctaat gctcctgttc cagaagaact agatctaaat gttaatccat
      121 ttaaataaac agttagagca tcgtttacag ccaatattcc tgttggattt acaaatgttt
      181 tatcaattgt tgcagaacta ttaattgtaa ctgtatgccc attagaaaca actgcactta
      241 atgaataata gctaggtatt gttgtagcag cagcccatga agaactatct gttgaagact
      301 cccaagttga taaatctccc caattgcctg ttgcttgact acgataatga taaaaagcag
      361 gcatctcata agctccaata tccggagttg aaagatttct tgtgtcacca ttaatatccg
      421 ttggaatact taaaggagtt cctgaatttt gcaggctacc attggtaggt aaataaatac
      481 tgccaatggg tgaaggatta tagaacatag gatttaaaac ataactgttc atatcatttg
      541 aagatgctgt ttgccagtct aataaagtag cctgagctgt tgaagattta taacctacat
      601 attgtgctcc agctcctaaa ctatttaaat agaaattatt ataattgctt gcatataaat
      661 tgcttgataa gtcatagaaa ccatatttaa caccagaacc acctcttgtt attgatataa
      721 tattgttttt atactgaact ccagaagatg cagtagaact catataaatt ccataagttg
      781 caccagctgt tgcaggggta taatctaaag aaacagtatt atgatagaac aaagcagtat
      841 ctgaaccaat agaatataaa ccataatggg taccattacc ttcatttgca taaataaggt
      901 tattggctat aagatttgga cttgcagcag gagcatctga agaaatataa atgccataca
      961 aggaagaagt acttgaaacc tgacctgcaa aagtgttatg aattttatta gaattaataa
     1021 acaatccctt attaccagtt gtagaataaa taccataaca ggaactagaa tttgttctaa
     1081 gtggacggtg aatattatta ttttctatga tgccattatt ttggttagaa aaatatattc
     1141 cgtaataata aaaatcttcc acactattat ttctaattgt attattagaa tttagagaac
     1201 tagctgcacc aatatttgta atgccataat accctcctac tattctgtta ttttctaaca
     1261 aattaccatt tccattatca tctgcaattg ttgcactagt taacgaaccg ctatatacaa
     1321 tacctgcata attagaggta gtagctgttg tgttgttcaa aatagtactt tttctaacaa
     1381 tattgctatc tgattggttc ataaattgaa taccataacc ataagtacct gcacttacat
     1441 ctattgttaa actatctata attaaatgat ctattccatt taatagtagt gctgttctag
     1501 tttcagaaac acttgcatta taggttacag tttcgccatt accattaata gttattgtgt
     1561 tggaagtaga agtaccataa atactattta taataatctg agaaggtaaa gcgtaaggac
     1621 cagagccact aactacattg aaaataacag gcccgtttat accacagctt atagagtcaa
     1681 tagcttcggc aaaagaagca aaattggtac cactagttgg taataattta ttgatagtaa
     1741 aagtaccgct taccatagct ggagaaacca cttgcactga agtagagtaa gtagtattac
     1801 cactacatgt aactgcacaa cggtaataag tagaagtaat ttgacttaca gattttagtg
     1861 gagatccatc agcaatatca atatctgtcc aaggtgcaga tcctgtagtt gaatattgcc
     1921 attgataggt ttgaccagta ccagaactat taccagttaa attaagagaa aatgtttcac
     1981 cattacaaac atttggagaa gagctggtag cactacctgc tgttggtggg gctatacaag
     2041 ctggtgccac atattcataa gcacctacat ctggagtact tccacttctg ctatttccaa
     2101 taatatcagt agttattcct aaggggctac ctaaattatc tataatagaa gaagtaaaag
     2161 aataatctcc actaattggg tcggtaaata ctgggtctgc aactaagcta tttaagtctt
     2221 gacttgtagc tgtttgccaa tctgataaac tggctcttgc agaagtttca taacctatac
     2281 tttgtgctcc actacctgca ctgcctaagt aaaaggcatt attattacta actaaaggtg
     2341 tagctggtgt ggctttatag atacaatatt ttgctccagt accaccacgg gttatataca
     2401 caatattatt tttaaactct atcccatctg cagtagttgt ttgccaaaaa ccggcagtag
     2461 tacctgcagt agcagctaca taatctaatg caatagtatt gtgatatgct tgcatatagg
     2521 cacctcctga attaaaaact ccataatgag tgccatcccc ttctatacta taaattaagt
     2581 tattataaaa tttatttggg ttggttacag tgccatcagc acttacatag atagcatagg
     2641 tagtgatagt atttacaggt aaagcggaaa ataagtgatg tattttattg ccactaacat
     2701 tcaacccagt acttacagat ataaaattca ttccataatt agttgtagaa aaagttgttc
     2761 tgttaggcct ttgaatatca ttatttttaa tctcagcatc tgcttgataa gcagcctgaa
     2821 taccgtaata attaaaatct tgtatagaat tgtttactaa tttattacca gcacaaagtg
     2881 ttgttgattc gccataagtt gaaataccat aatacccacc aatgatatta ttattttcta
     2941 tcaaatttcc attaccattg ttaccaattg atgtggcact ggttaaggag cctgttatta
     3001 ctatacctgc atagtttgta gaagtagaag aagtagagct tgtaatatta catgagccaa
     3061 tataattact atctgctgca tttattaaag taatacccca accataagtg cctgcactta
     3121 catcaatact taaattatta aggctaacaa aatcggctcc atttaaaatt attgctgatc
     3181 tatcggcaga aacacttgca ttgtaaacga ttgattggtt attaccatta atagtgatgg
     3241 tgttagatgg agatgcacca taaacatcgg gtattgaaat tggtgaagta attgtgtatg
     3301 gaccactacc tgctacaaca ttaaatacaa caggaccact aataccacat tttatagaat
     3361 taatagcatc tgcaaagctt gtaaaattac ttccacttgg tggcagggtt tggtctatag
     3421 taaaggtacc actaaccaaa gttggagtaa atatctttac agaatctgaa taagccgtat
     3481 taccgctaca agttacagca caacgataat aagttgtagt taattggtta agagtatctc
     3541 tttgttcaat atcggctgtt ccaatatctg tccaaggtgc agtaccagtt gtagaatatt
     3601 gccattggta ggtttggcca tcaccaaaac tatttccaga aaggtttaaa ataaaatcct
     3661 ctccactaca tgctgtatca atattactta ttgcaatacc tgcagttggt ggaactgtac
     3721 atggcggtaa gctataatta aagccaataa ctggccttct ataattacta cttgctaatg
     3781 ttgcaggcaa aacacctgtt gtattgttgg tgtatttacc ggtattatta ttgttgccac
     3841 tgcaaccaat accatattca taatcccaac ttatactatt actagtggta gaattagtat
     3901 attcaaaaaa caccgctaaa ttttgggttc cataagcaaa cgtggttgtt agtgaaaact
     3961 ctttaaaccc tgcagaagaa ccaacagcag aaactgggtt gccatcatat accaatgtgg
     4021 ctggtcctgt tgctgttgcc cagtctaaac ttccagctcc ccattctgta gaagaagttt
     4081 ctaacaaata aattttaaaa ttgggcgtac caagcattga accactagca gtagctcttt
     4141 taataaacat agaagtaagc gttgtgtttg ccacccctac aagttgtgta gcagggtaaa
     4201 taatagccgt tctattagtt gcatttgctg tagaagtgct atacataggc ccataagtag
     4261 ttgaagttaa attactacaa gttgcaattg accacatagg ttgtgctata gtactaccta
     4321 ttgcaagtaa tagaaaacaa aaaaagaata aaaatttttt catgacttta ttattattga
     4381 ataaaaaaca attaaaaaaa aggtgaacca aatattagtt gtacaattta atttactgta
     4441 atatgtagaa aaggtggttt gggtttacat atccattctt ttaaataatc tttaaaaaaa
     4501 actttatggt ttcttttagt gaaaaataaa aaataaatat tataaaagct atgttgagaa
     4561 atagacttaa tgaactggca atagtgtttt gtccattcca tttcattata agcaaaaaat
     4621 atcttattaa gatattgtag tttcataagc agctaaacca ttcttaaatt gggctactaa
     4681 agtatatttt ttttttttaa aaaaaaatat caattgttga ggtaaacaaa ggtttctata
     4741 ctaacagtta aaaaaattag gctaactatc ttcttttaag ttgctgctta acagaaaact
     4801 gtatatgtaa tataaattac atactactaa aacagtttat tacttaaaag aagaaaccag
     4861 aaaataaact aacttttagt taaatgtact ttagtcatgc tgtaataatg attttgtagt
     4921 tggtgcataa aatgtacatc tggatgatgt cttctatctt ctctatacca catttctatt
     4981 gtgctaaaat catcttttga aggtatcact aacctaaaag cttctttcat atatttaata
     5041 cttccatcct cattaacttg cttggtaaaa ttaaaattca ttaactgttg gtcgtttaat
     5101 ggtacagcat gtaaactttc tggtgcgtac caaaaaggtt gcacctcggt ggttacttct
     5161 acttgttttt catcgtggtt aaggttggtt acttctcctt cccacatttt accttcatac
     5221 tcaaccatca caaaatctcc tgtttgtaat tcattaaatt ttatcatata gcaaagtttt
     5281 agagtgagaa ggtaaaaata attttttata tagtcaaaaa aaaatcagca ccattaaatt
     5341 tatctcaata aatccaaatc gggttgtgca tatttatata ctttaaaata aatggttgtt
     5401 ttggtttgat gaacaccttc tatagtagaa attttattta caaattcaac caaaaaatca
     5461 ttattcttac acatcacatc aacctctaaa tcataatctc cactggttag tgctaaaaag
     5521 cttacttctt tatatttcga aattttatta gctacttttt cttttaatgt tgcagggcgt
     5581 acatacactg cgatatgggc atacgcatga aagccaactt tttctggatt taccctaccg
     5641 ataatattaa tagttccatc ttctattaat ttatttactc tcgttcttac tgttgcaata
     5701 gatacattta atttttctgc cattacagta aaactcattc taccatcttg ttgtaagcaa
     5761 gacagaatat taaaatcaag ggtgtccaat gaatgttgac tcataatttt ttaaaaataa
     5821 ataattacca ttaaaataaa aatagtaaaa atattttgtt atttctaaaa taattttagt
     5881 tttatagctc attttctaaa aaatttataa aaatattctt tagaaaatct atgattaaat
     5941 taaaaagtaa aataaaaaac aaacaatgaa ataccctgct attcaaaatt tcatcaatgg
     6001 aaaatttgta agtgcatcta caactaaaac aatggatgta atttctcctt taaatggtag
     6061 tcatttatca actgttccaa tgtcggctta caacgattta gataatgctg ttcgtgcagc
     6121 taaagctgca tttcctgcat ggagcaaaac acctataaaa gaaagagttc aagtattttt
     6181 tagatataaa actttattag aaaaacattt aaaagaatta gcagaactat gtacagaaga
     6241 aaatggcaaa acctatgctg aatctgttgc agaaattgaa aaatgtgtag agcttacaga
     6301 gtttgccact tcattacctc aattaattca aggagaaatt ttagaggtta gtaaaggtgt
     6361 agagtgtaga acagaacatg caccattagg cgttgttgcc tctattgtgc cttttaattt
     6421 tccttctatg gttccaaatt ggactattcc taatgcaata gctttaggta attgtatgat
     6481 tataaaacca agtgaaaaag taccattaag ctgtggtcgc ttagcagaaa ttttaaaaga
     6541 agcagggtta cctgatggtg tatttaatgt agttcatggc gatgtagaaa ttgtagaagc
     6601 tatttgcgac catcctggca tagaagcagt ttcttttgta ggttctacca aagtggctaa
     6661 aattgtttat caacgggcaa cacaaaattt aaaaagatgt ttggcattgg gaggtgcaaa
     6721 aaatcattta ttggttttac cagatgcaat acctgatatg actgcacaaa atgttgctgc
     6781 aagtatgagt ggttgtgcag gacaacgttg tatggcagca agtgcaatgg ttgctgtagg
     6841 cagtgtacaa catattattg ataaaatttg tgaggaagca aaaaaaataa ttccaggcaa
     6901 aaatttaggt gctgtaatta ataaagaatc gcaattaaga attgaaaagt atataactga
     6961 agcagagcaa caaggtgcaa aaattttagt agatggcaga aatacaaaag tagaaggaca
     7021 agagtatgga acttatgttg gtccaacagt tattgactat gtaacaccag aaatgagtgt
     7081 tgccaaagaa gaaatttttg ggccagttat ttctatcatg cgtactaaaa cagtagatga
     7141 agctttggca atagaaaatg caaaccctta tggcaatgct gcaagtgtgt ttacacaaaa
     7201 tggtggtatg gcacgttata taattgataa agcaagtgca ggaatgattg gtgtaaatgt
     7261 tggtgtacct gtaccacgtg agccatttag ttttggcgga tggaatgaaa gcaaatttgg
     7321 cgtaggcgat attacaggaa aaagtagtat tgagttttgg actaaactaa aaaaatctac
     7381 tacaaaatgg aatgcagaag caggcatcaa ctggatgagt taaataaacc tgaaagcgta
     7441 gcgtatttaa atattaatta ataacgataa tcaatataaa aaatgaaaac agaaacctta
     7501 tca
//
LOCUS       DAOQHP010000062         7494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5155_length_7494_cov_4.300712, whole genome shotgun sequence.
ACCESSION   DAOQHP010000062 DAOQHP010000000
VERSION     DAOQHP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5155_length_7494_cov_4.300712"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLY81_03240"
     CDS             complement(<1..947)
                     /locus_tag="PLY81_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF29275.1"
                     /translation="MALSLKKIFSNTILLTGCIYSFVYLLSCLTPFISPTVWSGFTFL
                     ALAFPILFCVMLFWLFIVFIFYKKYRWLFLIVTLLGSTNIFNIVAFNFPSKFMQEKQE
                     GNIRILSWNVQDFIDSQIYNDTPGSVRRKMMEFIKNTDADILCFQDFQEHTSTLFRSC
                     VKDITTQLNYPFKYFSRDFETKRYYAFIQYGTIIFSRYPIIDSGRTTYSNKNFSESLA
                     FADIKKGNDTFRIFNTHLKSMYLGLVMDTLAFKDEFIKDDLDFLMKNQDKFSRLKYYD
                     KVHAAQAKIVKEQLNKSKYPFIFCGDLNSVPSSYTYHTI"
     gene            complement(954..1874)
                     /locus_tag="PLY81_03245"
     CDS             complement(954..1874)
                     /locus_tag="PLY81_03245"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF29276.1"
                     /translation="MNTKSINKNKKPLLGANDNGLVLLVAINALIFIVLNFLRIAYTV
                     SADTVAIGDANFQKQILHWFVLHSNANLLAEKPWTILLYMFTNFSIWSLLSNLLWLWA
                     FGYILQDLAGNKKMIPVYLYGGFIGGLVFLLSLSFIPSLYQYVSSGYFLIGSGTSVMA
                     VAVATTTLAPNYKIFPLINGGIPLWILMVIYAALQFGSLAMGNTGITIAYLFSASIGF
                     LYIQQLNKGKDWGSWMFTLVNWINDIFNPEKKYPNLKKNSSREEVLNNKTTIAQEKID
                     IILDKINKHGYDKLSKEEKEFLNNASNSEV"
     gene            complement(1902..2600)
                     /locus_tag="PLY81_03250"
     CDS             complement(1902..2600)
                     /locus_tag="PLY81_03250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF29277.1"
                     /translation="MREFRPTRFEILPVVVKNLLIINGLVFFAQITIGEKFNVNDIFA
                     LHTWQSPLFKPWQFITHMFMHGNFGHLFSNMFALWMFGSILENVWGPKRFLTFYLICG
                     LGAALCHMIVLYYDMQPIVEAFKTLNFEEQQEILFSNRFRLNEATLGASGAVFGCLAA
                     FGYLFPNTQIYIYFLFPLKAKWFVLIYAGLELMQAVQNSAGDNVAHVAHLGGALVGFL
                     LVYYWNKTNKQSLY"
     gene            complement(2667..4088)
                     /gene="rlmD"
                     /locus_tag="PLY81_03255"
     CDS             complement(2667..4088)
                     /gene="rlmD"
                     /locus_tag="PLY81_03255"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNF29278.1"
                     /translation="MKKRRQPTILQNILVEDYAAEGKSLAKIDGKVIFIERAVPGDTV
                     DIQLFKNKKDWAEGFPINFISLSKDRVEPFCAHFQICGGCQWQMLPYQKQLAFKQKQV
                     SDNLKRIGKIDLPEILPIVGAEQTQFYRNKIEYTFANKRYVPDEELAYLKHNNLPFNN
                     EGSFGGFHAKGVFDKVVEIKKCYLQAEPTNEIRNAITAFCIQNNYPFYDIKNHTGWLR
                     NIMVRIATTGEWMVNIMLGFEKEKWRTDLLDFVLQKFPTITTLLYTINTKFNDSIYDL
                     TPVAYYGKGFIEEKLEQYKYNISPKSFFQTNTKQAEKLYNITRDFAELTGSETVYDLY
                     CGTGSIGIFVSNKAKKIIGVETVSDAVEVAKINASLNNITHAHFFAGDVIKVCDDIFF
                     ATHGKADVIITDPPRAGMHEKLITKLLEIAAPIIVYVSCNPATQARDLALLNAKYIVT
                     KVQPVDMFPHTHHIENVVQLKLR"
     gene            4245..6395
                     /locus_tag="PLY81_03260"
     CDS             4245..6395
                     /locus_tag="PLY81_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNF29279.1"
                     /translation="MKKILLALVLCISILAKADEGMWLPQLLQQLNEGRMKAMGMKIS
                     AKDIYDINKSSLKDAIVSFGGFCTGEIISSKGLILTNHHCGFRAIQAHSTIENNIIEN
                     GFWAKTHKDELGNKGLYVTFIKNIIDVTNKVLAGVNESMQPNERQAVIDKNIDNINSS
                     TKLGKFEKSFVREFFDGNMYYLFVTETFNDVRLVGAPPNSIGNFGVDTDNWVWPRHTG
                     DFSLFRIYANKNNEPADYSSDNIPYTPKRFLNISMKGIKENDFTMVFGFPGKTNEYLP
                     SEAVRQIAQINDPAKIYVRDKALAVLSKYMRNDMSIKIQYAAKYAGIANAWKKWQGEV
                     LGLTKTNAVEKKLQYEQVYNTKLKANTTLYNKYNGVLDKLINAYKRIEQYGLARDYYN
                     ETISKIELFSLANRIIRLKNVQKNGAEKFKNAVDEQIKSLPDFYKDFNAMVDKDMFIA
                     MMDIYLNKMPKEFISNTVMEMYKANGADLNKWADKIYSNCTLVNKEKTLDALNNNAAL
                     LIDNTLEDDLVKLMLAIRRHYSEAVAGELVKIQDEIDQLQRQYMKAQMEVMKDKKFYP
                     NANSTLRVTYGKVDGYSPADARVYKFQTYLDGVMEKYKPGDYEFDVNEKLRTLYKNKD
                     YGIYGVKGKMPVCFIASNHTTGGNSGSPALNAYGNLIGLNFDRGWEGTMSDINYDASI
                     CRNIMVDIRYILFIIDKYAGAGHLVKEMKLMYDK"
     gene            complement(6406..>7494)
                     /locus_tag="PLY81_03265"
     CDS             complement(6406..>7494)
                     /locus_tag="PLY81_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNF29280.1"
                     /translation="LIVILLLCYTFSQYEDAFEKLPIHWALWPAIGGIAVGLIGYYEP
                     KTLGVGYNNITDVLSGTLTFNVILSLCLLKFTSWAIAIGSGTSGGTLAPLLTIGGAIG
                     ALLGILGNQWFPAIGISIPLCALVGMSAMFAGASRAFLTSIVFALETTGQSNALLPLL
                     ATCTGAYCVSYLLMTNTIMTEKIARRGVQTPTSYEPDILDKTLVEELPLDDVIILNDA
                     MNVGEVRTWLKQEADAITSYFIVSDLDGTYKGLISYSSLFNMQHNNDLIIGDLVKRKN
                     IFIQPSANLKTAVETMSKEDVDILPVIEEDHIIGILTYQNILSIYKRSVFEKENKQVQ
                     ISLSRQRLKMLVRGQKIISILKSKKANT"
BASE COUNT         2754 a         1136 c         1110 g         2494 t
ORIGIN      
        1 tgaattgtat gataggtata gcttgatgga acactattta aatcgccaca aaaaatgaac
       61 ggatatttac ttttgtttaa ttgctctttt actattttgg cttgggctgc atgtacttta
      121 tcgtaatatt ttaatctgct aaacttatcc tgattcttca ttaaaaaatc taaatcatct
      181 tttataaatt catctttaaa tgctaaggta tccataacaa gccctaaata catagacttt
      241 aaatgggtat taaaaattct aaatgtatcg ttaccttttt ttatatctgc aaaagcaaga
      301 gactcagaaa aatttttatt ggagtaagtt gttcttccag aatctataat tggatatctt
      361 gaaaaaataa tggtaccata ttgtataaaa gcataatatc gttttgtttc aaaatctcta
      421 ctaaaatact taaatgggta gtttaattgg gtggttatat cttttacaca agatctaaaa
      481 agggtagaag tatgttcttg aaaatcttga aaacataaaa tatctgcatc ggtattttta
      541 ataaactcca tcatctttct tcttacacta cctggcgtat cgttataaat ttggctatct
      601 ataaaatctt gtacattcca acttaaaatt ctaatgtttc cctcttgttt ctcttgcata
      661 aattttgaag gaaaattaaa tgcaactata ttaaagatat tagtactacc taaaagtgta
      721 acaattaaaa acagccatct gtatttttta taaaatataa atacgatgaa tagccaaaat
      781 agcattacac aaaaaagtat tggaaatgct aatgctaaaa aagtaaagcc actccaaact
      841 gttggactta taaaaggagt aaggcaactt agtaagtaaa caaaactata aatacagcct
      901 gttaagagaa tggtattgct aaatattttt tttaaactta atgccatttt aaattacact
      961 tcactattac ttgcattatt taaaaactct ttttcttctt tgctaagttt atcgtagccg
     1021 tgtttattta ttttatctaa aataatatca attttttctt gtgctatggt tgttttatta
     1081 tttaaaactt cttctctgct actatttttt tttaggttag gatatttttt ttcaggatta
     1141 aatatatcat taatccagtt aaccaatgta aacatccaac tgccccaatc tttaccttta
     1201 tttaactgtt gaatatataa aaaaccaata gaggcactaa ataaataagc aatggttata
     1261 ccagtattgc ccatagctaa actaccaaac tgcaatgcag cataaataac catcaaaatc
     1321 cataaaggaa taccaccatt tattagtgga aaaattttgt aattaggtgc aagtgtagtg
     1381 gtagcaacag ctacagccat tacacttgtt ccgctaccaa ttaaaaaata gccagaactt
     1441 acatattgat ataaagaagg tataaaactt agagaaagta aaaaaactaa gccaccaata
     1501 aagccaccat acaaataaac aggaatcatt tttttattgc cagccaaatc ttgtaaaata
     1561 taaccaaatg cccaaagcca taataaattg gaaagcaacg accaaatgct aaagtttgta
     1621 aacatgtaaa gtaaaattgt ccatggtttt tcggctaaca aatttgcgtt ggagtgtaag
     1681 acaaaccaat gtaaaatttg tttttgaaaa ttagcatcac caatagcaac cgtatctgca
     1741 gaaactgtat aggcaattct taaaaaattt aaaacaataa aaattaatgc atttattgct
     1801 actaataaaa ctagcccatt atcattagct cctaataaag gttttttatt tttattaata
     1861 gattttgtat tcatagttgg cttaaaaaat acttcaaata gttagtaaag agattgcttg
     1921 ttggttttgt tccagtaata aactaataaa aagccaacta atgcaccacc taaatgtgcc
     1981 acatgtgcta cattatctcc agcagagttt tgaacagctt gcattaactc tagcccagca
     2041 taaattagta caaaccactt tgcttttaaa gggaataaaa aataaatata aatttgtgta
     2101 ttagggaata aatacccaaa cgcagctaaa caaccaaata cagcaccact tgcaccaagt
     2161 gtagcctcgt ttaatctaaa tctgttgctg aataatattt cttgttgctc ttcaaaattt
     2221 aatgttttaa atgcctctac aattggttgc atatcgtaat acaaaacaat catgtggcat
     2281 aatgcagcac ctaggccaca aattaaataa aacgtaagaa atctttttgg cccccaaaca
     2341 ttttctaata ttgagccaaa catccacaag gcaaacatat tactgaataa atgcccaaaa
     2401 ttgccatgca taaacatatg cgtaataaat tgccaaggtt taaacaatgg gctttgccag
     2461 gtatgcaaag caaaaatatc atttacatta aatttttcgc caatagttat ttgagcaaaa
     2521 aaaactagcc cgtttataat tagcaggttt tttacaacaa caggtaaaat ttcaaatcta
     2581 gttggtctaa attctctcat ttgttttata tattgaatta ccaaaagtat attttaaaaa
     2641 tttaaccata ttacttttgt aaaaatttat cttaatttta gttgaacaac gttttctata
     2701 tggtgtgtgt gtggaaacat atcaacaggc tgcacttttg taacaatgta tttggcatta
     2761 agtaaggcta aatctcttgc ttgtgtagca gggttacaac ttacatatac aataattggt
     2821 gcagcaattt ctaataattt agtaattaat ttttcatgca tacctgcacg tggtgggtca
     2881 gtaattatta catctgcctt gccgtgggtt gcaaaaaaaa tatcatcgca aactttaata
     2941 acatctcctg caaaaaaatg agcatgggtt atattgttta aagaggcatt aatttttgca
     3001 acttctacag catctgatac agtttcaaca ccaataattt ttttagcttt attgcttaca
     3061 aaaattccta tactacctgt accacaatat aaatcataca ctgtttcact acctgttagt
     3121 tcagcaaaat ctcttgttat attgtataat ttttctgctt gttttgtatt ggtttgaaaa
     3181 aaagattttg gactaatatt atatttatat tgttctagct tttcttctat aaaacccttg
     3241 ccataatagg ctactggcgt taaatcgtat atactatcat taaactttgt attaatagta
     3301 taaagtaatg tagtaatggt tggaaatttt tgaagtacaa aatctaacaa atctgttctc
     3361 cacttttctt tctcaaaacc aagcattata ttcaccatcc attcaccagt ggtggcaatt
     3421 ctaaccatta tatttcttag ccagcctgta tgatttttta tatcataaaa aggataatta
     3481 ttttgaatac aaaaggcagt aattgcattt ctaatttcat ttgtaggctc tgcttgcagg
     3541 tagcattttt ttatttcaac tactttatca aaaacacctt ttgcatgaaa gccaccaaaa
     3601 ctaccctcgt tgttaaaggg caaattatta tgttttaaat aagccaattc ttcatctggc
     3661 acataccgtt tattagcaaa tgtgtattca attttgtttc tgtaaaactg tgtttgctct
     3721 gcaccaacta taggtaaaat ttctggtaaa tctattttgc caatacgttt taaattatcg
     3781 cttacttgtt tttgtttaaa agccaattgt ttttggtaag gaagcatttg ccattggcat
     3841 ccaccacaaa tttgaaaatg agcacaaaat ggttctactc tatctttaga aagggaaata
     3901 aaatttattg gaaagccctc ggcccaatct tttttatttt taaatagttg aatatctaca
     3961 gtatcaccag gtactgccct ttcaataaaa attactttac catctatttt tgcaagcgat
     4021 tttccttcgg ctgcataatc ttctaccaaa atattttgta aaatggtggg ttgccttctt
     4081 tttttcacaa ggcaaatttc attgaaaata ttgatataca gaaatgtaac atttaaagaa
     4141 tgattatttc tgaaacaaat aaaaatgtaa gtttaataac tttaattttg agttatactt
     4201 gcaaaagtta atattttatt ttttgaacat taaaattgat tagaatgaaa aaaattcttt
     4261 tagcccttgt attatgtatt agcatccttg caaaagcaga tgaaggaatg tggttgcctc
     4321 aattattgca acaacttaat gaaggtagaa tgaaagcaat gggtatgaaa atttcggcaa
     4381 aagatattta cgacatcaac aagagtagct taaaagacgc tattgttagc tttggtggtt
     4441 tttgcactgg cgaaataatt tctagcaaag gattaatatt aactaatcat cattgtggct
     4501 ttagagcaat acaagcacac tcaaccatag aaaataatat tatagaaaat ggcttttggg
     4561 ctaaaaccca taaagatgaa ctaggcaata aaggcttgta tgttactttt ataaaaaata
     4621 ttattgatgt aaccaacaaa gttttagcag gtgtaaatga aagcatgcaa ccaaacgaaa
     4681 gacaagctgt aattgataaa aacattgaca atataaattc atccaccaaa ttaggcaagt
     4741 tcgaaaagtc ttttgttcga gaattttttg atggcaatat gtattactta tttgtaacag
     4801 aaacttttaa cgatgtaaga ttagtgggtg caccaccaaa ctctattggc aattttggtg
     4861 tagatacaga taattgggtt tggccaaggc ataccggaga tttttcctta ttcagaattt
     4921 atgcaaataa aaataatgag cctgcagatt actcttctga taatattcct tataccccaa
     4981 aaaggttctt aaatatatct atgaagggta ttaaagaaaa tgattttaca atggtatttg
     5041 gttttcctgg taaaacaaac gagtacttac caagcgaagc tgttagacaa attgcacaaa
     5101 taaacgaccc tgctaaaatt tatgttagag ataaagcatt agctgtatta agtaaatata
     5161 tgcgtaacga tatgtctata aaaatacaat atgcagctaa atatgctggt attgctaatg
     5221 cctggaaaaa atggcaagga gaagttttag gcttaaccaa aacaaatgca gtagaaaaaa
     5281 aactacagta cgaacaagta tataatacaa aacttaaagc caatacaacg ctttataata
     5341 agtataatgg tgtattagat aaattaataa atgcatataa acgcatagag caatatggac
     5401 ttgcaagaga ttattataac gaaactataa gtaaaattga actgtttagt ttagcaaata
     5461 gaataattag attaaaaaat gtacaaaaaa atggagcaga aaagtttaaa aatgcggttg
     5521 atgaacaaat aaaaagctta cccgattttt ataaagattt taatgcaatg gttgataaag
     5581 atatgtttat tgcaatgatg gatatttatt taaacaaaat gcccaaagaa tttatatcta
     5641 atacagtaat ggaaatgtat aaagccaatg gtgcagactt aaacaagtgg gctgataaaa
     5701 tatatagcaa ttgtactttg gttaataaag aaaaaacctt agatgcttta aataataatg
     5761 ctgctctttt aatagataat actttagaag atgatttagt taagctgatg ctggcaatta
     5821 gaaggcatta ttcagaagct gttgcaggcg aattggttaa aatacaagat gaaattgatc
     5881 agcttcaaag gcagtatatg aaagcacaga tggaggtaat gaaagataag aaattttacc
     5941 ccaatgctaa tagtacatta agagttactt atggtaaagt agatggttat tcgccagccg
     6001 atgctagagt gtacaaattt caaacatatt tagatggtgt gatggaaaaa tataaaccag
     6061 gagattatga atttgatgta aatgaaaaat taagaaccct atacaaaaat aaagactatg
     6121 gaatttatgg agtaaaagga aaaatgcccg tttgctttat cgcatcaaat catacaacag
     6181 gaggcaatag tggaagccct gctttaaatg cttatggcaa tttaataggt ttaaactttg
     6241 atagagggtg ggaaggcacc atgagtgata ttaattatga tgccagtatt tgtagaaata
     6301 ttatggtaga tattcgttac attttattta ttatagataa atatgctggt gcaggacatt
     6361 tagttaaaga aatgaaatta atgtacgata aataattttg gactattaag tattagcttt
     6421 tttagatttt aaaatagaaa taattttttg gccacgaacc aacattttta aacgttgcct
     6481 acttaaagaa atctgtactt gtttgttctc tttttcaaat acagaacgtt tgtatataga
     6541 taatatattt tggtaagtta aaataccaat aatatggtct tcttcaataa caggtaatat
     6601 atctacatct tctttagaca tggtttcaac tgctgttttt aaattagcag atggttgtat
     6661 aaaaatattt tttcgtttta ctaaatcacc aataattaaa tcattattat gttgcatgtt
     6721 aaataagctt gagtagctaa ttaacccttt ataagttcca tctaaatcac ttacaataaa
     6781 ataacttgta atagcgtctg cttcttgttt tagccaagtt ctcacttcac caacattcat
     6841 tgcatcattt aaaataatta catcatctaa tggcagttct tctactaagg ttttatctaa
     6901 aatatcaggt tcataagagg taggtgtttg tacacctctt cttgcaattt tttctgtcat
     6961 aatggtatta gtcattaata aataagaaac acaataagca cctgtacaag ttgccagcaa
     7021 gggtaataat gcattggatt gaccagtagt ttctaaagca aaaacaattg aggttaaaaa
     7081 tgctcttgat gcacctgcaa acattgcaga cataccaacc aatgcacata atggtataga
     7141 aatgccaatt gcaggaaacc attgattacc taaaatgcca agcaatgcac caatggcacc
     7201 accaatagtt aataatgggg ccaaagtacc accagatgtt ccactaccaa tagctatagc
     7261 ccaagatgta aactttaata aacataatga tagaataaca ttaaaagtta aagtgccaga
     7321 taaaacatct gtaatattat tataaccaac acccaaagtt ttaggttcgt aataacctat
     7381 taagccaaca gcaataccac caattgcagg ccacaaagcc caatgtatag gtaatttttc
     7441 aaaagcatct tcatattgag aaaaggtata acagagaagc aatatgacaa taag
//
LOCUS       DAOQHP010000063         7483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5171_length_7483_cov_3.807754, whole genome shotgun sequence.
ACCESSION   DAOQHP010000063 DAOQHP010000000
VERSION     DAOQHP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5171_length_7483_cov_3.807754"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(557..784)
                     /locus_tag="PLY81_03270"
     CDS             complement(557..784)
                     /locus_tag="PLY81_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29281.1"
                     /translation="MKRVLVTVILILFVWQLNAQCSICTKTASQLGERPAAALNNAIV
                     YLMMTPLLIIGFVGYKWWKREKEIMKEEQSA"
     gene            864..2093
                     /locus_tag="PLY81_03275"
     CDS             864..2093
                     /locus_tag="PLY81_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF29282.1"
                     /translation="MKNNFPLSGFSSVALHAANNSNNAYAHSTPIYATSTFVFDNAQQ
                     GMERFESEDKEKIYSRWSNPTFKVAEETIAAFEAFNIKTNNGNTIQLKALLHASGQAA
                     MTTMFLSCLQVGDTVLSHYSLYGGTYELMYKVLRQAGINIEIVNFKNLNEVEDRIKQN
                     KTIKFIHIETPANPTIQCVDIEAISTLAKQFNIKVSVDNTFATPYLQQPFQFGVDFVL
                     HSTTKFLNGHGTSIGGVLLGSDIDFMKQKVWKWFALLGGNANAFDAFMLINGMKTLEL
                     RMERHCSNAKQVASFMQQHHAIAQVNYNGLESHVDYALTLKQMKHAGALLSFELKGGI
                     ESGKKFIDNLKMCTRAVSLGTVDTLVSHPASMTHYSVPKEERIKFGITDGLIRVSIGI
                     ENVEDILNDFDQALHSV"
     gene            complement(2106..2975)
                     /gene="era"
                     /locus_tag="PLY81_03280"
     CDS             complement(2106..2975)
                     /gene="era"
                     /locus_tag="PLY81_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNF29283.1"
                     /translation="MKSGFVNIFGKPNAGKSTLLNALMGEKLAIVSHKVQTTRHRIKG
                     FLTEPEKYQIIFSDTPGIIEAKYRLHEKMMLAVKNALEDADVALLIVDVNDSLDEADA
                     IFKQLNLKVPCILILNKIDEAKNGKIAEAEKFFSDKPYAKKIIKISALQQHHLSKLLN
                     AILELLPEGAPFYNEDDLTDLPTRFFVGELIREKIYTLFEDEIPYHVAVLVNEYKEKD
                     TLVKIQADIIVQRETQKSIIIGGGGKMIKQIGTIARKDIEQFIGSKVFLELFVKVKPK
                     WRDNELQLKEYGY"
     gene            complement(3047..3118)
                     /locus_tag="PLY81_03285"
     tRNA            complement(3047..3118)
                     /locus_tag="PLY81_03285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3084..3086),aa:Met,seq:cat)
     gene            complement(3170..3901)
                     /locus_tag="PLY81_03290"
     CDS             complement(3170..3901)
                     /locus_tag="PLY81_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNF29284.1"
                     /translation="MKKIVCLLLCIIGIINLNAQNTNTVVNDPNAELRDVDEFKSIEV
                     SSGFIVYISQGKTQAVAVSCSNEKNNEDIITTVENGVLKIYRPKSTFKLLHKNQKFRA
                     YITVKNIEKLDISGACIVNLVNPIKADDLEIDISGASIIKGEVNAKHLNSDVSGASIS
                     TLKGNVASARINVSGASILKAFNLLVEEYDINVSGASIANIYVTQKLIASASGTSSIK
                     YKSTTENIKVNSTGLSNISEYNKDN"
     regulatory      3980..4085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4147..6504
                     /locus_tag="PLY81_03295"
     CDS             4147..6504
                     /locus_tag="PLY81_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29285.1"
                     /translation="MKKFMGTLLFLFITAFAFAQKTKITGKVIDEQTNLPLYGATVKL
                     NSLITITDEQGYFIFKNVKKQPFTILVSNIGYLDYSSEIKNLESSNDLIIQLKRQVYF
                     LDALEVTSIRAADKSPFSKNNLYKEQLAKLNVGQDLPFVLNTLPSVVINSDAGNGIGY
                     TGIRIRGTDATRINVTVNGIPFNDAESQGSFFVNMPDLVSSTNTIQVQRGVGTTTNGT
                     GAFGATINLSTNEVIEKPYTEINNTVGSFSTWKSTIKAGTGLLNKHFTLDTRLSKILS
                     NGYVQRASSNLQAFYVSAAYIAKNTSLRLNIFSGNEKTYQAWNGIDEATLKSNRTYNS
                     SGTEKLGEPYKNETDNYKQTHYQLFFNHKANQKWQFNIATFLVRGLGYYENYKANQKF
                     SKYGLPDVIIGASTITKTDLVRQLWLDNYFYGTIFSSEYKKNKTNLTIGGGWSKYDGK
                     HFGKLPWAAIGIEKDYQYYNNPATKTEFNIYSKLQQQVSHSITLFSELQCRNVQQLMN
                     GFRNNPSLLVNRKFDFVNPKIGLTFIKKGWQTYASYAMVGKEPNRDDFEAGTITQPKA
                     EILHNIELGTEKKTSKYNLAANIYVMLYKNQLVLTGKINDVGAYTRINVPKSYRVGIE
                     LQGSYVFSNWLSMMANITISKNKIQQFTEYIDDYDNGTQVQINQSNKTIALSPSFISS
                     ASINFLPLKNIEFSLIGKFVGKQYLDNTESNLKQLNSFYTQDVRLIYSIKNCNLSFQV
                     NNILNTLYQPNGYTFNYIYGGKLIIENYYFPMAGINFLAGVNIKL"
     gene            6554..7147
                     /locus_tag="PLY81_03300"
     CDS             6554..7147
                     /locus_tag="PLY81_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF29286.1"
                     /translation="MNVKKIVVVGAESTGKSTLCTMLAQHYKTEWCAEFAREYLLKNG
                     TNYNFESLEIIAKGQLALEDECNNKVSKNWQPIADGKFINSHTLTTINHQPLLFIDTN
                     MYVMKVWCEFVFNKCHQFILDEIVSREYDLYLLCNIDLPWIADELREYPNEEPRQMLN
                     NIYKDILINQSTPWAEIKGSNYINRFKQAVKAINTIL"
     gene            complement(7154..>7483)
                     /locus_tag="PLY81_03305"
     CDS             complement(7154..>7483)
                     /locus_tag="PLY81_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29287.1"
                     /translation="DLIEFINMFRFKRNRQILALQKRLEVQEREKYIDHRFKKLLITK
                     LTGLQQPQLDTFMLNYRPEYYFLQRLNDLELGYYIQQCYQAYKLNLPNPYKTQERNNN
                     ERDENLN"
BASE COUNT         2636 a         1104 c         1196 g         2547 t
ORIGIN      
        1 ctgttctttg ttgttgctat tcagtagatc cttcgctacg ctcaggatga caaaaaaggg
       61 ggaataggat gacaaaaaaa agtattgtca ttccgaaaga agtgaggaat ctttacttct
      121 ctgtcattcc gaatgaaatg aaaaaaagta ttgtcattcc gagcctgcga ggaatctctt
      181 tgttcttaac ctttagatcc ttcgcttcgc tcaggatgac ataaaagggg ggaaaaggat
      241 gacatgattg aacgaaacga aaaataaaaa ttctctgtca ttccgagcct gcgaggaatc
      301 tatctgttct ttgttggtgc tacaatagat ccttcgcttc gctcaggatg acaagaaaag
      361 gggagcagga tgaaatgtag tgaatgaagt gaagcaaaaa atgcttgtca ttccgagctt
      421 gcgaggaatc taaatgtact ttggtacaaa acctcagtag atccttcgct acgctcagga
      481 tgacaagaaa aagtgtgcag aggattgacc ttaaaaagga agaataatat ggaaagatgg
      541 tagcaaaaaa taagaattag gcactttgtt cttccttcat aatttctttt tctcttttcc
      601 accatttgta accaacaaag ccaataatta ataaaggcgt catcattaaa tatacaattg
      661 cattatttaa ggcagcggca ggtctttctc ctaattgcga tgctgtttta gtgcaaatag
      721 aacattgtgc attaagctgc caaacaaaaa gtattaatat tacagttaca agtactcttt
      781 tcattatatg gcaaagataa aacaatccat tctaaaagca gttttgtact gcattttgta
      841 attttgattt tataaataat tgtatgaaaa ataattttcc actttcaggg tttagctctg
      901 ttgcattaca cgcagcaaat aatagtaaca atgcttatgc tcattccaca ccaatttatg
      961 ctacctcaac ctttgtgttt gataatgcac aacaaggaat ggaacgtttt gaaagtgaag
     1021 ataaagagaa aatttactca cgttggagta acccaacatt taaggttgcc gaagaaacaa
     1081 ttgctgcatt cgaagctttt aacattaaaa caaataatgg taatactata caacttaaag
     1141 ccttgttaca tgcaagtggc caagcagcaa tgaccaccat gtttttaagc tgcttacaag
     1201 taggcgatac tgttttgtct cattactcct tatatggtgg cacttacgag ttaatgtata
     1261 aagttttaag gcaagctgga ataaatattg aaattgtaaa ttttaaaaac ttgaatgaag
     1321 tagaagatag aataaaacaa aataaaacta ttaagtttat tcatattgaa acaccagcta
     1381 atcctactat tcaatgtgta gatatagaag ctatttctac attagcaaaa cagtttaata
     1441 ttaaagtatc tgttgataat acttttgcaa caccttattt acaacagcct tttcaatttg
     1501 gtgttgattt tgttttacat agtactacaa agtttttaaa tgggcatggt acttctattg
     1561 gtggtgtttt gttaggaagt gatattgatt ttatgaagca aaaagtatgg aaatggtttg
     1621 ctttattagg aggtaatgca aatgcttttg atgcttttat gctaattaat ggaatgaaaa
     1681 ctttagaatt aagaatggag aggcattgta gcaatgcaaa gcaagtagcc tcttttatgc
     1741 aacagcacca cgccattgca caagtgaatt ataatgggtt agaaagccat gttgattatg
     1801 cacttacatt aaagcaaatg aaacatgctg gtgctttact gagctttgaa ctaaagggtg
     1861 gtatagaatc gggtaaaaaa tttattgaca atcttaaaat gtgtacaaga gcagtttcct
     1921 taggtactgt agatacttta gtttcccatc ctgcaagtat gacacattat agtgttccta
     1981 aagaagaaag aataaaattt ggaattactg atggcttaat aagggttagt attggtatag
     2041 aaaatgtaga agatatttta aacgattttg accaagcttt acattctgtt taaatttttt
     2101 aaaacttaat acccatactc tttcaattgt agttcattat ctctccattt aggttttact
     2161 tttacaaata gttctaaaaa aactttactg ccaataaatt gctcaatatc ttttcttgcg
     2221 attgttccaa tttgttttat catttttccg ccgccgccaa ttattattga tttttgcgtt
     2281 tctctttgca caataatatc tgcttgaatt tttactaagg tatctttttc tttatattca
     2341 ttaactaaaa cagcaacatg gtaaggtatt tcatcttcaa ataaagtata aattttttct
     2401 cttatcaatt cgccaacaaa aaatctagta ggtaaatcgg ttaaatcatc ttcattataa
     2461 aaaggggcac cttctggcag caattctaaa attgcattta gtagtttaga taaatggtgt
     2521 tgttgtaatg cagaaatttt aattattttt tttgcataag gcttatccga aaaaaatttt
     2581 tcagcttctg ctattttacc atttttagct tcatcaattt tatttaatat taaaatgcaa
     2641 ggtactttaa ggtttagttg tttaaatatg gcatctgctt catctaagct atcatttaca
     2701 tctacaatta ataaagctac atctgcatct tctaaagcat tttttactgc aagcatcatt
     2761 ttttcatgaa gcctgtattt agcttcaata atgccaggtg tatcagaaaa aataatttga
     2821 tacttttcag gttctgttaa aaaacctttt attctatgcc tggttgtttg aaccttgtga
     2881 gatacaattg ctaatttctc ccccattaat gcattcagta aagtactttt tcctgcattg
     2941 ggtttaccaa aaatatttac aaaaccagat ttcatattgt ataaaaatag tatgctttaa
     3001 ggaaatggta taaaaaaata aagcccaaca ttgggcttta ttttagttgc gaggataggg
     3061 ttcgaaccta tgaccttcgg gttatgagcc cgacgagcta ccgctgctct acctcgcggt
     3121 attatggagt gcaaaagtag cggtatgcaa tatactacca aaatttattt taattgtcct
     3181 tattatactc tgaaatattg gataaacctg tactgttaac ttttatattt tctgttgtgc
     3241 ttttatattt gatgctactt gtgccgcttg cagaggcaat tagtttttga gtaacataaa
     3301 tattagcaat gcttgcccca cttacattta tatcatactc ttcaaccaat aagttaaatg
     3361 cttttaaaat acttgctcca ctaacattta tacgagcact agcaacatta cctttaagtg
     3421 tagatatgct tgcaccactt acatcactat tcaagtgctt ggcattaact tcgcctttta
     3481 taatacttgc accagaaata tctatctcta agtcatcagc ttttattggg ttaaccaagt
     3541 ttacaataca agcaccagaa atatctaatt tttctatatt ttttacggtt atataagccc
     3601 taaatttttg gtttttgtgt agtagtttaa atgtagattt tggtctgtaa attttcaata
     3661 caccattttc aacagtagta ataatatctt cattattctt ttcattactg caacttacag
     3721 caacagcttg tgttttaccc tgagaaatat atacaataaa accactagat acctcaatgc
     3781 ttttaaattc atctacatct cttaattctg cattagggtc gtttacaact gtattagtat
     3841 tttgtgcatt taaattgatt ataccaataa tacaaagcag taagcatact atctttttca
     3901 ttgtgctatt tttatattta acgaataaag agaataaagg ttacattata tcattaattt
     3961 atcttgtaca tttgcaccaa acaattcctc ggggtgctta ttcttttttt aaaagaatgg
     4021 ctgagattac acccgtagaa cctgaacagg gtaattcctg cgaagggaag gcggcttcta
     4081 tatttatagt caactttcta gagcgttatt cctgttccca tttttaaaat tataaaattt
     4141 tttacaatga aaaaattcat gggaacattg ctgtttttat ttattacagc atttgcattt
     4201 gcacaaaaaa caaaaattac cggcaaagtt attgatgaac aaacaaattt gccattgtat
     4261 ggtgccacag ttaaacttaa cagtttaatt actattacag atgaacaagg ttactttatt
     4321 ttcaagaatg taaagaaaca gccatttact attctagtaa gcaatattgg ttatttagat
     4381 tactcaagtg agataaaaaa tttagagagt agtaatgatt taataataca attaaaaagg
     4441 caagtgtatt ttttagatgc tttagaagta acatctatta gagctgcaga taaatctcct
     4501 tttagcaaaa acaatttata taaggagcaa ttggcaaagt tgaatgttgg acaagattta
     4561 ccttttgtat taaacaccct accatctgta gtaattaatt cagatgcagg caatggcata
     4621 ggttatactg gtattagaat tagaggtaca gatgccacca gaataaacgt aactgtaaac
     4681 ggaattcctt ttaatgatgc agaaagccag ggatcatttt ttgtaaatat gccagatttg
     4741 gtttcctcta caaacacaat tcaggtacaa agaggagttg gtacaacaac caatggtaca
     4801 ggagcatttg gtgctacaat aaacttaagt acaaatgaag taatagaaaa accttatacc
     4861 gaaattaata atacagttgg ctcttttagc acctggaaga gtacaataaa agcaggtact
     4921 ggattattga ataagcattt tactttagat accaggctta gtaaaatttt gagtaacggc
     4981 tatgtacaaa gagcaagtag taacttacaa gccttttatg tatcagcagc atatattgca
     5041 aaaaatacat ctttaaggct aaatattttt tctggtaatg aaaaaactta tcaggcatgg
     5101 aatggtatag atgaagctac tttaaaaagc aacagaacat ataactcaag tggtaccgag
     5161 aaattaggag agccgtataa aaacgaaacg gataattaca agcaaactca ttatcaactt
     5221 tttttcaatc ataaagcaaa tcaaaaatgg cagtttaata ttgctacttt tttagtgaga
     5281 gggttgggtt attatgaaaa ctataaagca aatcaaaagt ttagcaagta tggtttacct
     5341 gatgtaataa ttggtgcatc tacaattacc aaaacagact tagtaaggca attatggctt
     5401 gataattatt tttatggaac tattttttca tctgaatata aaaaaaataa aacaaattta
     5461 actattggag ggggttggag taaatacgat ggtaagcatt ttggcaaact gccttgggca
     5521 gccattggta tagagaagga ttatcaatat tataataatc ctgcaacaaa aacagaattt
     5581 aatatatatt caaaattaca acaacaagta tctcattcta taacattatt ttctgaattg
     5641 caatgtagaa atgttcaaca attaatgaat ggatttagaa ataatccttc tttattggta
     5701 aatagaaaat ttgattttgt taatcctaaa ataggtttaa cttttattaa aaaaggctgg
     5761 caaacttatg caagctatgc tatggtaggt aaagaaccca atagagatga ttttgaagct
     5821 ggaacaatta cacagccaaa ggcagaaatt ttacataata tagaattggg aacggaaaag
     5881 aagacatcta aatataattt ggctgcaaac atatatgtaa tgttatataa aaaccaatta
     5941 gtgttaacag gtaaaataaa tgatgttgga gcctatacca gaattaatgt acccaaaagt
     6001 tatagagttg gtatagaatt acaaggaagc tatgtatttt caaactggtt aagtatgatg
     6061 gcaaatatta ccatcagtaa aaataaaata caacaattta cagagtatat agatgattat
     6121 gataatggta ctcaggttca aattaaccaa tctaataaaa ccattgcttt gtcaccatct
     6181 tttataagta gtgccagtat caatttttta cctctaaaaa atattgaatt tagtttaatt
     6241 ggtaaatttg ttggtaagca atatttagac aatacagaaa gtaatttaaa acaattaaac
     6301 agcttttata ctcaagatgt aagattaatt tactctatta aaaattgcaa cctttcgttt
     6361 caggtaaata atattttaaa cacattgtac caacccaatg gctatacttt taattatatt
     6421 tatggtggaa aattaattat tgaaaactat tattttccaa tggctggaat aaatttttta
     6481 gcaggggtaa atataaaatt atagttagtt aaaaatagtt atacttgcta cattatagta
     6541 atgcacaatt agtatgaatg taaaaaaaat tgtagtggta ggtgccgaaa gtactggaaa
     6601 aagtactttg tgtacaatgt tggcacaaca ctataaaaca gaatggtgtg ccgaatttgc
     6661 aagagaatat ttattaaaaa atggtacaaa ttataatttt gaaagcctcg aaattattgc
     6721 aaaagggcag cttgctttag aagatgaatg caacaacaaa gtctctaaaa actggcaacc
     6781 aatagcagat ggaaaattta taaatagcca taccttaact actataaacc atcaaccatt
     6841 attatttata gataccaata tgtatgtaat gaaagtgtgg tgtgaatttg tttttaataa
     6901 atgtcatcaa tttattttag atgaaattgt aagcagagaa tatgatttgt acttgctttg
     6961 caatattgat ttaccttgga ttgccgatga attaagagaa tatcctaatg aagaacctag
     7021 gcaaatgctg aataatattt ataaagatat tcttattaac caatcaaccc cttgggcaga
     7081 aataaaaggt agtaattata ttaacagatt taaacaagca gttaaagcaa ttaatacaat
     7141 tctttaaaaa aaattaattc aaattttcat ctctctcgtt attatttctt tcttgtgttt
     7201 tatacggatt aggcaggttt aatttgtatg cctgataaca ttgttgtata taatagccca
     7261 gttctaaatc atttaacctt tgtaaaaaat aatattcagg tctatagttt aacataaaag
     7321 tatctaactg tggttgctgc aaaccagtta gtttagttat aagtagtttc ttaaaacgat
     7381 ggtcaatata tttttctctt tcttgaactt ctaatctttt ttgcaatgcc agtatttgcc
     7441 tgtttctttt aaacctaaac atattaataa actctattaa atc
//
LOCUS       DAOQHP010000064         7411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5226_length_7411_cov_3.321506, whole genome shotgun sequence.
ACCESSION   DAOQHP010000064 DAOQHP010000000
VERSION     DAOQHP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_7411_cov_3.321506"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PLY81_03310"
     CDS             <1..1087
                     /locus_tag="PLY81_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29288.1"
                     /translation="AETPVFVDKEFTQKMIDCCENIVDVITGFNFKTLTSHGIPANVR
                     VANENNHSHFIAFDFGVCENNEGKLEPQLIEMQGFPTLFAYQVLLDQVTRKYFSIPDN
                     YTPYLSNLNEESYLNLLKEIIVGNHPAENVILLEIFPHQQKTRVDFYCTEAYLGIKTI
                     CLTELILEGKKLYYEKDGKKIEVKRIYNRLIFDDLQQQTAEVQEKGKILLSDLEVEWV
                     PHPNWFYRISKYTLPYINHPFVPETKFLTDLIELPTDLENFVLKPLFSFAGQGVIIDV
                     TKEDISKVKDPENWILQKKVNYADIIKTPDGPAKAEVRIFYFWKDGEARPIATFNLAR
                     ISKGKMIGVRYNKGKEWVGGHIAYFEK"
     gene            1376..1660
                     /locus_tag="PLY81_03315"
     CDS             1376..1660
                     /locus_tag="PLY81_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29289.1"
                     /translation="MYKYLTFTILTSVLCITASAGEFNPKPNKSIANKNVTATIQTTK
                     KPLGFIKKTNTLAALKLNYQEVVKVKIDSNTVVEYVPIFVEKKIKIKRNS"
     gene            1999..6285
                     /gene="rpoC"
                     /locus_tag="PLY81_03320"
     CDS             1999..6285
                     /gene="rpoC"
                     /locus_tag="PLY81_03320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNF29290.1"
                     /translation="MAIKKDVNKTKPGFSKITISLASPDSILERSFGEVLKPETINYR
                     TYKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     KLVVPVVHIWYFKSLPNKIGYLLGMSSKKLETIVYYERYVVIQGGVKESLGTGDLLTE
                     EEYLDIIDTLPKENQYLPDEDPEKFIAKMGAEAVHDLLERLDLDELSYQLRNAAANET
                     SQQRKADALKRLSVVEAFRDSSARISNRPEWMVMQYIPVIPPELRPLVPLDGGRFASS
                     DLNDLYRRVIIRNNRLKRLMEIKAPEVILRNEKRMLQEAIDSLFDNSRKSNAVKAEGG
                     RALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKMHECGLPKDMAAELFKP
                     FIIRKLLERGIVKTVKSAKKYVDKKEAIVWDILENILKGHPVLLNRAPTLHRLSIQSF
                     QPRLIEGKAIQLHPLVTAAFNADFDGDQMAVHVPLSSAAILEAQLLMLSSHNILNPQN
                     GTPITLPSQDMVLGLYYITKIKKSTKEEPVKGEGKTFYSLDEVVIAYNENVVDLHAGI
                     KVKTTVRENGQLVNKLIDTTVGRVLFNQHVPKEVGYVNALLTKKSLREIIGEIIKITN
                     VPKTAKFLDDIKTLGFRMAFKGGLSFSVNDLIVPEERNELLENAKAEVDEVWESYNMG
                     LITNNERYNQVVDIWGRADMIITETLIHKMTTDKQGFNSVFMMLDSGARGSKTQVKQL
                     VGIRGLMAKPRKSGSSGSEVIENPILSNFKGGLNVLDYFISTHGARKGLADTALKTAD
                     AGYLTRRLVDVAQDVVISEEDCGTLRGIAISALKDNEDIIEPLADRIEGRTSLHDVLH
                     PVTDALIIKAGEEISSEVAKEIENSGIEAVEIRSVLTCESKRGVCVKCYGKNLATGYI
                     TQSGDAVGIIAAQSIGEPGTQLTLRTFHVGGVAGSSSVESSLNAKFDGTVQFDGLRTV
                     SSVNNEGNKVNIVIGRTGEVRTMDVKNDRLLITNNVPYGSTLLVKDGQQIKKGDVICT
                     WDPFNNVIVAEQNGTVEFESLIDGITFREESDDQTGHREKVIIETKDKTKIPTVIVAG
                     KDNKHYNLPVGSHLAVDEGEEVKAGQVLVKIPRILGKLRDITGGLPRVTELFEARNPG
                     NPAVVCEIDGIVTFGNVKRGNREIAVESKDGMIKKYLVPLSRQILVQDGDFIKAGAPL
                     SDGQIAPADILAIKGPFAVQEYVVNEIQEVYRLQGVKINDKHIEVIVRQMMKKVEIVD
                     AGDTKFLEEDLEDRVDFIAENDRIFDKKVVVDAGESTKMRAGKIITLRELREENSILR
                     RADKKTVEVRDAKPATATPVLLGITKASLGVQSWISAASFQETTKVLSTAAINGKVDY
                     MTGLKENVITGHLIPAGTGQREFDNLIVGSKEEYELLTAAREAMSFDDDE"
     gene            6453..6541
                     /locus_tag="PLY81_03325"
     tRNA            6453..6541
                     /locus_tag="PLY81_03325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6487..6489,aa:Ser,seq:gct)
     gene            6645..>7411
                     /locus_tag="PLY81_03330"
     CDS             6645..>7411
                     /locus_tag="PLY81_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF29291.1"
                     /translation="MATISTDQISFLVALIGADYVLYDTESLEKYSRDETEKLKFYPD
                     VIVKPKTTNEVAAIMQFCNQHLIPVTPRGAGTGLSGGALATLGGVILSTERMNSIIEI
                     DTRNMFVITEPGVITEDLMNAVKEKNLFYPPDPSSKGSCFIGGNIAENSGGPKAVKYG
                     VVKDYVLNVELVLPTGEVIWTGANVIKNATGYNLTQLIVGSEGTLGIVTKIVLKLLPL
                     PKVDLLMLVPFKNLNNASDAVSAIFNAGFTPSALELI"
BASE COUNT         2737 a         1010 c         1353 g         2311 t
ORIGIN      
        1 agccgaaact cccgtttttg tagataaaga atttactcaa aaaatgatag attgctgcga
       61 aaacattgta gatgttataa ctggctttaa ttttaaaaca cttacctctc atggcatacc
      121 tgcaaatgtt cgtgtagcaa atgaaaataa ccactctcat tttatagctt ttgattttgg
      181 agtttgtgaa aacaacgaag gaaaattaga acctcagtta atcgaaatgc agggttttcc
      241 tactttattt gcttatcaag tactattaga ccaagtaaca agaaaatatt ttagcattcc
      301 agataactac accccttatt taagtaattt aaacgaagaa tcctatctaa accttttaaa
      361 agaaattatt gttggcaatc atccagcaga aaatgtaata ctattagaaa tatttcctca
      421 tcagcaaaaa acaagagttg atttttattg taccgaagca tatttaggta taaaaactat
      481 ttgtttaact gaattaattt tagaaggcaa aaaattatac tacgaaaaag atggaaaaaa
      541 aattgaagta aaaagaatat acaataggtt aatttttgat gatttacaac aacaaacggc
      601 cgaagtacaa gaaaaaggca aaattttatt aagtgattta gaggtagaat gggtaccaca
      661 tcctaattgg ttttacagaa taagtaaata cactttacct tatatcaatc atccttttgt
      721 accagaaact aaatttttaa cagacttaat agaattgcca acagatttag aaaactttgt
      781 tttaaagcct ttattctctt ttgcagggca aggggtaatt attgatgtta ctaaagagga
      841 tataagtaaa gtgaaagatc cagaaaattg gattttacaa aaaaaagtaa actatgccga
      901 tattattaaa acacctgacg ggccagctaa agctgaagtg agaatatttt atttttggaa
      961 agatggagaa gctagaccaa tagcaacctt taatttagcc agaataagta aaggtaaaat
     1021 gataggagta cgatataata aaggaaaaga atgggttggt ggacatatag cttattttga
     1081 aaaatagctt cttttaattt tatttataac ccgataaagc attgttttat cgggattttt
     1141 attttatgta ttctacaatt tggaaaacta agccaaaaat ggaaaaattt aataaattta
     1201 tatttaaatt tttaattaaa aaaatataat tattcactat taatcaatga gttatttaat
     1261 tttataattg tattgttttg gtttggcata gtatttctta taataatatt ggttcagtaa
     1321 tccaatattt agtgaaaagc taaaacaatt tagcaaactt taatatttaa ataaaatgta
     1381 caagtattta actttcacta tcttaacttc tgttttatgc attactgcaa gtgctggcga
     1441 atttaaccca aagccaaata aaagcattgc aaacaaaaat gttactgcaa caatacaaac
     1501 aaccaaaaag cccttaggct ttattaaaaa aacaaatact ttagctgctc taaaactaaa
     1561 ttatcaagag gtagtaaaag taaaaataga cagtaatact gtagtagaat atgttccaat
     1621 ttttgtagag aaaaaaatta aaattaaaag aaacagttag aaataaccat accagtttat
     1681 taaataatac gttaggcctt ttatgcaaaa cataaaaggc tttttgttag gtgaaaacca
     1741 tgtatttaca gcttttaaat aattcaaaaa attacttttt catttcaaaa actatacata
     1801 ccttcgccgc cttaaacaat ttgggatatt gtatcttttt caaaaaattt tgtgttaaac
     1861 tgcagcttgt cttacaacaa aattgtgtaa gaatgtacaa gtaatgctga gtgcctcacc
     1921 aacattcagt tagctatgga aaccaaaaca ggaatgagaa gatagactat ttcaaaataa
     1981 aatttcatct actataatat ggctattaaa aaagatgtaa acaaaaccaa acctggtttt
     2041 tcaaaaatta caatcagcct agcctctcca gattctattt tggaaagaag ttttggcgaa
     2101 gttttaaaac ctgaaactat caactatcgt acgtataaac cagaaagaga tggtttattt
     2161 tgcgaaagaa tttttggccc tgtaaaagat tatgaatgtg cttgtggtaa atacaaacgt
     2221 attcgttata aaggcattgt ttgtgatcgt tgtggcgtag aagttacaga aaaaaaagta
     2281 agaagagaaa gaatggggca tataaaactg gtggttccgg ttgtgcacat ttggtacttc
     2341 aaaagtttac ctaataaaat tggttattta ttaggaatga gttctaaaaa attagaaaca
     2401 attgtttact acgaacgtta tgtagtaatt caaggtggtg tgaaagaaag tttaggtaca
     2461 ggagatttat taactgaaga agagtatttg gatattatag ataccttacc aaaagaaaat
     2521 cagtatttgc cagatgaaga cccagaaaag tttattgcta aaatgggtgc agaagcagtg
     2581 catgatttat tagaaagatt agaccttgat gaattaagct atcaattacg taatgctgca
     2641 gcaaacgaaa catctcaaca acgtaaagca gatgctttaa aacgtttgag tgttgtggaa
     2701 gcttttagag actcttctgc aagaatttca aaccgtccag aatggatggt tatgcaatat
     2761 attccagtta ttccaccaga attaagacca cttgtacctt tagatggtgg acgttttgct
     2821 tcttctgatt taaacgattt atatcgtcgt gtaatcatta gaaacaatcg tttaaaacgt
     2881 ttgatggaaa ttaaagctcc cgaagtgatt ttgcgtaacg aaaaacgtat gttacaggaa
     2941 gctattgata gcttatttga taatagccgc aaatcaaatg cagtaaaagc cgaaggtggt
     3001 agagcgttaa aatctttaag tgatgtttta aaaggtaaac aaggtcgttt ccgtcaaaac
     3061 ttattaggta aaagggttga ctattctggt cgttctgtaa ttgtagtagg acctgaatta
     3121 aaaatgcatg aatgtggatt gcctaaagat atggctgcag aattatttaa gccatttatt
     3181 attcgtaaat tattagaaag aggtattgtt aaaactgtaa aatctgctaa aaagtacgta
     3241 gataaaaaag aagcaattgt ttgggacatt ttagaaaata ttctaaaagg ccatcctgtt
     3301 ttattaaatc gtgcccctac ccttcacaga ttatctattc agtcttttca accaaggtta
     3361 attgaaggaa aagctataca attacaccca ttagttacag cagctttcaa cgctgacttt
     3421 gatggtgacc aaatggctgt acacgttcct ttaagtagtg ctgcaatttt agaagctcaa
     3481 ttattaatgt tgtcttctca caacatttta aatcctcaaa atggtacacc aattacctta
     3541 ccttctcaag acatggtact tggcttatac tatatcacaa aaattaaaaa atctaccaaa
     3601 gaagaacctg taaaaggcga aggaaaaaca ttttatagtt tagatgaagt tgtaattgct
     3661 tacaatgaaa acgtggttga tttacatgct ggtattaaag taaaaactac agttcgtgaa
     3721 aacggacagt tagtaaacaa actaattgat actacagtag gacgtgtatt atttaaccaa
     3781 catgttccaa aagaagtagg ttatgtaaat gcacttttaa ccaaaaaaag tttaagagaa
     3841 attattggag aaattattaa aattaccaat gtacctaaaa ctgctaagtt cttagatgat
     3901 atcaaaacct taggttttag aatggcattt aagggtggtc tttcatttag tgtaaacgac
     3961 ctaatagtac cagaagaaag aaacgaactt ttagaaaatg ctaaggcaga agttgatgaa
     4021 gtttgggaaa gttacaatat gggcttaatt accaataacg aacgttataa ccaagttgta
     4081 gatatttggg gtagagccga tatgattatt actgaaacat taattcataa aatgactaca
     4141 gataaacaag gatttaactc tgtattcatg atgttggata gtggtgccag aggaagtaaa
     4201 acacaggtaa aacagttagt tggtattaga ggcttaatgg ctaaaccacg taaaagcgga
     4261 tctagcggtt ctgaggtaat tgaaaatcca attctttcga actttaaagg cggcttgaat
     4321 gtattagact actttatatc tacccacggt gctcgtaaag gtttggcgga tacagcctta
     4381 aaaactgcag atgctggtta cttaacacgt agattagttg atgtagcaca agacgttgta
     4441 atttctgaag aagattgtgg tacactacgt ggaattgcga ttagtgcatt aaaagataat
     4501 gaagatatta ttgaaccatt agcagataga atagaaggta gaacatcatt acacgatgtt
     4561 ttacaccctg taacagatgc cttaattatt aaagcaggtg aagaaattag ttcggaagtt
     4621 gccaaagaaa ttgaaaatag cggaattgaa gcagtagaaa tacgttccgt tttaacctgc
     4681 gaatctaaac gtggtgtttg tgttaaatgc tatggcaaaa acctagcaac aggttatatt
     4741 acacaaagtg gcgatgctgt aggaattatt gcagcccaat ctattggtga acctggtaca
     4801 cagcttacac ttcgtacctt ccacgtaggt ggtgttgcag gcagctcttc tgttgaatct
     4861 tctttaaatg ctaagtttga tggtactgtt cagtttgatg gtttacgtac tgtatctagt
     4921 gtaaataatg aaggtaacaa agtaaatata gttattggtc gtactggtga agtaagaaca
     4981 atggatgtta aaaacgaccg attactaatt acaaataatg ttccttatgg ctctacatta
     5041 cttgtaaaag atggtcaaca aattaaaaag ggtgatgtta tttgtacatg ggatccattt
     5101 aacaatgtaa tagttgcaga acaaaatggt actgtagaat ttgaaagttt aatagatgga
     5161 attactttta gagaagaaag tgatgaccaa acaggacacc gtgaaaaagt aattattgaa
     5221 acaaaagata aaactaaaat tccaacagtt atagtagcag gaaaagataa taagcactat
     5281 aacttacctg taggtagcca cttagcagtt gacgaaggag aagaagttaa agctggtcag
     5341 gttttagtta aaattccacg tatattaggt aagttaagag atattacggg tggtttacct
     5401 cgtgttacag aattatttga ggcaagaaac ccaggcaatc ctgcagttgt ttgtgaaatt
     5461 gatggtatag ttacttttgg taatgtaaaa cgtggtaata gagaaattgc agttgaatct
     5521 aaagatggaa tgattaaaaa atatctagta ccattatctc gtcaaattct tgttcaagat
     5581 ggtgacttta ttaaagcagg tgcaccatta tctgatggac aaattgcacc agcagatatt
     5641 cttgcaatta aaggtccttt tgccgtacaa gaatatgtag taaacgaaat tcaagaagta
     5701 tatcgtttgc aaggtgtaaa aattaacgat aaacatattg aagtaattgt tcgtcagatg
     5761 atgaaaaagg tagaaatagt agatgctggc gatacaaaat tcttagaaga agatttagaa
     5821 gacagagtag attttattgc agaaaatgat agaatttttg ataaaaaagt agtggtagat
     5881 gctggcgaaa gcactaaaat gagagcagga aaaataatta ctcttcgtga actaagagaa
     5941 gagaattcta ttttaagaag agcagataaa aaaactgttg aagtgagaga tgctaaacct
     6001 gcaactgcaa ctccagtatt acttggtatt actaaagcct ctttaggtgt acaaagttgg
     6061 atttctgcag cttctttcca agaaacaact aaagtattaa gtacagcagc aattaatggt
     6121 aaagtagatt atatgacagg tttaaaagaa aacgtaatta caggtcattt aattcctgca
     6181 ggtaccgggc agcgtgaatt tgataattta attgtaggta gtaaagaaga atatgaatta
     6241 ttaactgcag caagagaggc aatgtctttt gatgatgatg aatagtttaa aattattctt
     6301 tcaatataaa aatgcaggcc ttaaaaagct tgcatttttt attccaaatt ctttaaaaaa
     6361 aaataaaaaa actatttaca aatagttttt tctatataca atttgctatt atatttgcag
     6421 ccctttcgtt atagcagaat agggataatg ttggtgagat ggatgagtgg ctgaaatcaa
     6481 cagtttgcta aactgtcgta cgggtcaaac tgtaccgagg gttcgaatcc ctctctcacc
     6541 gcaaagagat tgatttttat cagtctcttt ttttattctt taaacctaat gcaatattta
     6601 ttatctataa ttcttatcac atacatttgc tttagaaaaa tattatggct acaatatcaa
     6661 cagatcaaat atcatttctt gttgcattaa tcggtgctga ttatgtttta tatgatacag
     6721 aatctttaga aaaatatagt agagatgaaa cagaaaaatt aaaattttat ccagatgtta
     6781 ttgtaaaacc aaaaacaaca aacgaggttg ctgcaatcat gcagttttgt aatcagcatc
     6841 ttattccagt aactccacgt ggtgctggca caggtttaag tggaggtgca ttggctactt
     6901 taggaggtgt gattttaagt acagaaagaa tgaatagtat tattgaaata gatactagaa
     6961 atatgtttgt tataaccgaa cctggggtaa ttactgaaga cttaatgaat gctgttaaag
     7021 agaaaaattt attttatcct ccagacccta gcagtaaagg tagttgtttt attggtggca
     7081 atattgcaga aaatagtggc ggcccaaaag cagttaaata cggtgttgta aaagattatg
     7141 ttttaaatgt agagcttgtt ttgccaactg gtgaagtaat ttggacagga gccaatgtta
     7201 ttaaaaatgc aacaggatat aacttaacac aattaattgt aggcagcgaa ggcacattag
     7261 gtattgtaac aaaaattgtt ttaaaactat tgccattacc taaggtagat ttattaatgc
     7321 ttgtaccatt taagaactta aataatgcaa gcgatgcagt gagtgccatt tttaatgcag
     7381 gttttacacc aagtgcatta gaacttattg a
//
LOCUS       DAOQHP010000065         7408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5227_length_7408_cov_3.840473, whole genome shotgun sequence.
ACCESSION   DAOQHP010000065 DAOQHP010000000
VERSION     DAOQHP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5227_length_7408_cov_3.840473"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLY81_03335"
     CDS             complement(<1..253)
                     /locus_tag="PLY81_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HNF29292.1"
                     /translation="MIAVSEAKKLIAENCIYHKIIPIKIGDALDYVLAENIYAPFNSP
                     HFIQSAMDGYAFNFDNWNGKDEFTIVGEVQAGKCYDGPIN"
     gene            complement(280..1728)
                     /locus_tag="PLY81_03340"
     CDS             complement(280..1728)
                     /locus_tag="PLY81_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF29293.1"
                     /translation="MNILLTGANGYIGMRLLPVLVESGHEVTCVVRDKNRFKPAKDLL
                     DKINIIEFDFLHPENAVPCFKNKQFDAAYYLIHSLSDTSNTLKEYELRAAGCFVLVAL
                     LTNVKQIIYLSGISNDDKLSNHLTARKVVKDVFIKSGIPYTIFEAGIIVGSGSISFEI
                     IRDLTEKLPVMIVPRWLNSKCQPIAIRNVIYYLQHCVHNEKTYFKTFEIGGPDIFSYK
                     QMLLGLAKVRGYKRYIITFPVLFPGFSVYWLNLTTSANFTIAKQLVHSMKNDVVCKEY
                     AIREIIPQYLFTYEEAIKLAFARIEQNMVVSSWTDSASSSLHRLDVSQYIEVPVNGCF
                     KDKKWIEISTDEIEEVANRFFSIGGEQGWYYADSLWRIRGVFDKLIGGVGLSRGRRSK
                     VDIEAGDTIDFWRVILADKKNHRLLLFAEMKLPGEAWLEFSIINNNNRSILKQTATFR
                     PKGIMGRNYWYSMLPFHYFIFRNMIKRIAGKK"
     gene            complement(1739..2722)
                     /gene="moaA"
                     /locus_tag="PLY81_03345"
     CDS             complement(1739..2722)
                     /gene="moaA"
                     /locus_tag="PLY81_03345"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNF29294.1"
                     /translation="MLTDKFNRTHTYLRISITDKCNLRCTYCNPIDLPKGYFSVSNKM
                     SPDEIEQIATTFVKNGITKIRLTGGEPLVRKDAKEIILRLAKLNVELAITTNGVYVNE
                     FIEVFKQANIKTVNVSLDTLKQQKNLLITGRNEFDKVHENINLLLQNDFTVKVNVVVM
                     KNTNDNEILDFVEWTKNQPVHVRFIEFMPFSGNNWSNDKVFNYKEILQLIKTKYKYEN
                     LQQDKSDTAMKFKAVNHKGTFAIISTMSKPFCGGCNRLRLTTDGKMKNCLFSKTEVDI
                     LTALRNKENIEPLIQQCVWDKEEIQGGQFNSINGINDALAIKNRSMIFIGG"
     gene            complement(2738..3439)
                     /locus_tag="PLY81_03350"
     CDS             complement(2738..3439)
                     /locus_tag="PLY81_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNF29295.1"
                     /translation="MNNIFNNGNKDNSDYIKRFVEKDTGDVQVYSPMDPPDAYKPQGV
                     EPVPYNELHAMMQALMDEHKITLEKINDFEATLLSIKKDGVNKESNKKLGDFFAFIDE
                     KIVLHNLKEERILFPLIHDRMIDNGEHGTGKIPDTAVDLLENDHIKMMELATLTFSLM
                     GITSRITDPISNALLMDTAIEQGLSLVEMLRLHIFREDNVVFPLAHKYLTPSDYEKIT
                     HQMKKYFSIDLPSKN"
     gene            complement(3455..4534)
                     /locus_tag="PLY81_03355"
     CDS             complement(3455..4534)
                     /locus_tag="PLY81_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNF29296.1"
                     /translation="MKENKSIQISTTIKEGIKTLSPAYFALVMATGIISIGAYIMQFK
                     WIATTLFFLNNIFFVLLWALTILRVVWYFKFLKTDLTNHLTGTGFFTTVAGTCVLGIQ
                     YALMYERFTIATVLLVWGILLWLFLTYTIFTTLTTQQNKPTLDKGITGGWLTAIVATQ
                     SVSILSALVATHIGQPYRVEINFFALSMWLWGGMFYIWMISLIFYRYTFFKFSPDDLA
                     PPYWINMGAMAISALGGSVLILNAPGAPFLDSLLPFIKGFTVFYWATGTWWIPMLIIL
                     AIWRHIIKRYPLKYDPLYWGAVFPQGMYAVCTYRMSKALNMPFLHYFYQAFIYLAIIA
                     WTITFLGLLKFLYSKLVNQNHFILK"
     gene            complement(4568..6097)
                     /locus_tag="PLY81_03360"
     CDS             complement(4568..6097)
                     /locus_tag="PLY81_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF29297.1"
                     /translation="MVEWILPNLPNKNSYMNSTSTPRASYAALVLSTFAFIVCFSAWM
                     INGVLVTYLVDQRVFNWSPIQIGWLMGVPVLVGSVFRLPVGILTDKFGGRWVMTFILI
                     LCAIPMYFLSYANNYSTFLLLSFCFGLGGTSFAAGVAYTSLWFSKEKQGTALGIFGAG
                     NIGAAFTTIFAPSLLKKITNNGADIENWRALPQLYAALLVITAIVFFIFAKNKISPVS
                     KSMVQRLMPLKEVRVWRFGLYYFFVFGAFVALSQWLIPYYVNVYSLSIVSAGFMAAAF
                     SLPAGLFRAAGGWLSDKVGARMLLIWTLSVCLICMIFLFIPRMEIQAPGQGIMSKKAG
                     KVMAVSNSEILIGSDVYTLQDKAGDASNVTIRFGIHHDQEGFLFLPTTTFHQVPNVKV
                     GEEVKKGDLIAKGVSLIYFQANKWIFTTLVFIIGIMMGLGGAAVFKHISDYYPNNIGT
                     VGGIVGVLGGLGGFCGPIIFGFLLKQTGIWTSCWMFLAALVMLCIVLQRTAIKALLKN
                     N"
     gene            complement(6063..6509)
                     /locus_tag="PLY81_03365"
     CDS             complement(6063..6509)
                     /locus_tag="PLY81_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29298.1"
                     /translation="MFLSIVSDEQLMTNQIVKSILLIILSGGIVLNIFRIFRNPTRNK
                     KLLHIVLLLIISTIFYFVFNAYKIEAALLKNPQYIIGTTKGFCKTYGQSNIDFEYEIN
                     GKKFEGCNTYSPIPQSSIITNGGRYLVRYSNKYPEGGRMDFTKPAK"
     gene            complement(6512..7240)
                     /gene="narI"
                     /locus_tag="PLY81_03370"
     CDS             complement(6512..7240)
                     /gene="narI"
                     /locus_tag="PLY81_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNF29299.1"
                     /translation="MNLFDNFILVVLPYVAIATFLIGSIYRYTSRKFQVSSLSSQFLE
                     GKKLFYGSVPFHWGIVFIFLGHLIAFLFPRGVLAWNSQPVRLMILEISAFMFGISMLV
                     GLINLFIRRYTTPRLRYVTNYMDFAIYVLLILQTFAGLWIAYNYRWGSSWFSTLLTPY
                     LYSIFKFNPETSAIVLLPLMVKLHIAGAFIIIGILPFSRLIHLLVLPLNYIWRPYQQV
                     MWYWDKNKVRSHKTPWSLQRPKNT"
BASE COUNT         2539 a         1292 c         1004 g         2573 t
ORIGIN      
        1 tattaattgg gccatcataa catttacctg cttgcacttc tcctacaatt gtgaattcat
       61 cttttccatt ccaattatca aaattaaatg catatccatc cattgcagat tgtatgaaat
      121 gtggagaatt aaaaggagcg taaatgtttt ctgctaaaac atagtcaagt gcatctccaa
      181 ttttaatagg tataatttta tggtagatac aattttctgc aattaatttt tttgcttcag
      241 atactgcaat catttttcct ttctaaatat ttatacaaat tattttttgc ccgcaattcg
      301 tttaatcata tttctaaaaa taaaataatg aaatggcaac atagaatacc agtaattacg
      361 acccattata ccctttgggc gaaaggttgc cgtttgtttt aagatagacc gattgttatt
      421 atttatgata gaaaattcta accatgcctc accgggtaat ttcatttcgg caaataaaag
      481 aaggcgatgg ttttttttat cggctaaaat tacacgccaa aaatctattg tatcgccagc
      541 ttcaatatct actttacttc ttcttcctct acttaatcca acacctccaa ttaatttatc
      601 aaaaacgcct ctaattctcc aaaggctatc tgcataatac caaccttgtt cgccaccaat
      661 actaaaaaat cggttagcca cttcttcaat ttcatctgta gatatttcaa tccatttttt
      721 atctttaaaa caaccattaa ctggcacttc aatatactgg cttacatcta acctatgtag
      781 gctgctggat gcagagtctg tccagctaga aacaaccatg ttttgttcta ttcttgcaaa
      841 agctaatttt atggcttctt cataagtaaa taaatattgt ggaattattt ctcttatagc
      901 atactcttta caaacaacat catttttcat actatgcact aattgttttg ctatagtaaa
      961 atttgccgat gtggttaaat ttaaccaata cactgaaaag ccaggaaata aaacaggaaa
     1021 tgtaattata tatcttttat accctctaac ttttgctaag cctaataaca tttgtttata
     1081 gctaaatata tctggccctc caatttcaaa ggttttaaaa tatgtttttt cattgtgtac
     1141 acaatgttgt aaataataaa ttacattacg tattgcaatt ggttggcatt tagagtttag
     1201 ccaacgaggt acaatcatta caggaagttt ttctgtaagg tctctaataa tttcaaatga
     1261 aatgctaccg gagccaacaa taattcctgc ttcgaaaatg gtataaggaa tacccgattt
     1321 tataaacaca tcttttacaa cttttcttgc agttaagtgg ttcgataatt tatcatcgtt
     1381 gctaatgcca cttaaatata ttatttgttt tacattggtt aataaggcaa ctaatacaaa
     1441 acatcctgct gcccttagtt catattcttt taaagtattg gaggtatcgc ttaaggaatg
     1501 tataaggtaa taagctgcat caaattgttt atttttaaaa catggaacag cattttcggg
     1561 atgcaaaaaa tcaaactcaa taatatttat tttatctaat aaatcttttg ctggtttaaa
     1621 cctgttctta tctcttacca cacaagtaac ttcatgaccc gactctacca atactggtaa
     1681 taacctcatt cctatataac cattagctcc tgtaagtaat atattcatac gctattgttt
     1741 aacctccaat aaaaatcata cttctatttt taattgctaa agcgtcattt attccattta
     1801 tagaattgaa ttgtccccct tgtatttctt ccttatccca aacgcattgt tgaattagtg
     1861 gttcaatatt ttctttgttt cttaatgctg ttaaaatatc tacctcagtt ttagagaaca
     1921 aacagttttt catttttcca tcggtagtta atcttagtct attacaacca ccacaaaatg
     1981 gcttactcat tgtactaata attgcaaatg tgcctttatg gtttactgct ttaaatttca
     2041 ttgcagtatc acttttgtct tgctgtaggt tttcgtattt atattttgtt ttaataagtt
     2101 gaagtatttc tttataatta aatactttat cattactcca attatttccc gaaaatggca
     2161 taaactctat aaacctaaca tgaacaggtt ggttttttgt ccattctaca aaatctaaaa
     2221 tttcgttatc attagtattt ttcattacta ctacatttac ttttactgta aaatcatttt
     2281 gtagtagaag gttaatattt tcatgtactt tatcaaactc atttctgcct gtaattaata
     2341 aatttttttg ttgctttaat gtatctaaac ttacattaac ggttttaata tttgcttgtt
     2401 taaatacttc aataaattca ttaacgtaaa cgccattggt tgtaattgcc aattcaacat
     2461 ttaatttagc taatcgtaaa ataatttctt ttgcatcttt tcttactaag ggttcgccac
     2521 ctgtaagcct tattttagta attccgtttt ttacgaaagt ggttgcaatt tgttcaatct
     2581 catcggggct cattttatta gatacagaaa aatatccttt tggtaaatca ataggattac
     2641 aataggtgca acgtaaattg catttatctg ttatagaaat tcttaaataa gtatgtgttc
     2701 tattaaattt atcggttagc ataactctat attataatca atttttagag ggcaggtcta
     2761 tagagaagta ctttttcatt tgatgtgtaa ttttttcata atcgcttggt gttaggtatt
     2821 tatgggctaa tggaaaaact acattgtctt ctctaaagat atgcaatctt agcatttcta
     2881 ccaaagacaa gccttgttct atagcagtat ccatcagcaa tgcattagat ataggatctg
     2941 taattctaga agttataccc attaagctaa aagttaaggt tgccaactcc atcattttta
     3001 tatgatcatt ctctaacaaa tctactgctg tatctggtat ttttcctgtt ccatgttctc
     3061 cattatctat cattctatca tgaattaatg gaaataaaat tctttcttct tttaaattat
     3121 gaagtacaat tttttcatca ataaatgcaa aaaaatctcc aagcttttta ttgctttctt
     3181 tattcactcc atcttttttt atggataata atgtagcttc aaaatcatta attttttcaa
     3241 gtgttatttt atgttcatcc attaatgctt gcatcatagc atgtaattca ttatagggta
     3301 ctggttctac accttgtggt ttgtaagcat cgggtgggtc cattggtgag taaacttgta
     3361 catctcctgt atctttttct acaaatcttt taatataatc gctattgtct ttatttccgt
     3421 tgttaaaaat gttgttcata gtttgtattt atttttattt taaaataaaa tgattttggt
     3481 ttaccaattt agaatataaa aactttaata atcctaagaa ggttattgtc caggcaataa
     3541 ttgccaagta aataaatgcc tgataaaaat aatgcaaaaa gggcatattt aaagctttag
     3601 acattcgata ggtgcaaact gcatacatgc cttgcggaaa cacagctccc caatataaag
     3661 gatcgtattt taagggataa cgtttaataa tatgtcgcca aatggctaat ataatcagca
     3721 ttggaatcca ccatgtgcca gttgcccaat aaaaaactgt gaagcctttt ataaaaggca
     3781 ataacgaatc taaaaaaggt gctcctggtg catttaaaat taaaacactt cctccaagtg
     3841 ctgatattgc cattgcaccc atatttatcc aataaggagg agctaaatca tcgggagaaa
     3901 atttaaaaaa agtatatcta taaaagatta aagaaatcat ccagatataa aacatgcctc
     3961 cccaaagcca catagataat gcaaaaaaat taatttcaac tctgtagggt tgaccaatat
     4021 gcgtagctac taacgcactt aaaatagaaa ccgattgtgt ggcaacaatt gctgtaagcc
     4081 aaccacccgt tattccttta tctaatgtag gtttattttg ttgtgttgtt aatgttgtaa
     4141 aaatggtata ggttaaaaaa agccataata aaatacccca aactaataaa actgttgcaa
     4201 ttgtaaatcg ttcatacatt aaagcatact gtattcctaa aacacaagtt cctgcaactg
     4261 ttgtaaaaaa acctgtgcct gtaaggtgat tggttaaatc ggtttttaaa aatttaaaat
     4321 accatacaac tcttaaaata gttaatgccc ataatagcac aaagaaaata ttgttaagaa
     4381 aaaagagagt agtagcaatc catttaaact gcataatata tgcaccaata gagataatgc
     4441 ctgttgccat tactaaagca aagtaagcag gagaaagtgt ttttatacct tcttttattg
     4501 ttgtactaat ttgtatgctt ttattctctt tcatttataa ttagtaacat tcttttgttt
     4561 attgctatta attatttttc agtaaggcct ttattgctgt acgttgtaat acaatacata
     4621 gcataactaa cgctgctaaa aacatccagc aagaagtcca aatgcctgtt tgttttaata
     4681 aaaaaccaaa aattattggg ccacaaaatc ctcctaaccc acctaaaact cctacaatac
     4741 caccaacagt tccaatatta ttaggatagt agtctgaaat atgtttaaat actgctgcac
     4801 ctcctaaacc catcattata ccaattataa aaaccagtgt ggtgaaaatc catttatttg
     4861 cctgaaaata aataagagaa accccttttg ctattaaatc tcctttctta acctcctcac
     4921 caacttttac atttggtacc tgatgaaaag tagttgttgg taaaaacaaa aaaccttcct
     4981 gatcatgatg aataccaaac cttatagtaa cattggatgc atcacctgct ttgtcttgaa
     5041 gggtgtacac atcgcttcca attaatattt cagagttgga aacggccatt actttaccag
     5101 cttttttact cataatgcct tgtcctggtg cttgtatttc cattctaggt ataaataaaa
     5161 aaatcataca aataagacaa acactcaatg tccaaattaa taacattctt gcacctactt
     5221 tatccgataa ccagccacct gctgccctaa acaagcctgc tggcaaacta aaagctgcag
     5281 ccataaaacc agcagataca atagataaag aataaacatt tacatagtaa ggaattaacc
     5341 attgagacaa tgcaacaaaa gctccaaaaa caaaaaagta gtataaccca aaacgccaaa
     5401 cccttacttc ttttaatggc ataagccttt gtaccattga tttagatact ggcgaaattt
     5461 tgtttttagc aaatataaaa aacacaatag ctgtaattac taataaggca gcatatagct
     5521 gtggtaatgc tctccagttt tcaatatctg ctccattatt agttattttt tttaataagg
     5581 atggtgcaaa aattgtagtg aatgctgcac ctatattacc tgctccaaaa atgcccaatg
     5641 ctgtaccttg tttttctttg ctaaaccata aacttgtata ggcaacgcca gcagcaaaag
     5701 atgtgcctcc taaaccaaaa caaaaactaa gtaataaaaa tgttgagtag ttgtttgcat
     5761 aggataaaaa gtacatgggt attgcacata agattaatat aaatgtcatc acccatctgc
     5821 caccaaactt atctgttaaa atacctactg gcaatctaaa aacagagcct actaaaaccg
     5881 gtacacccat taaccatcca atttgaatag ggctccagtt aaaaactctt tgatctacta
     5941 aataagttac taatacacca ttaatcatcc aggcagaaaa acaaacaata aatgcaaatg
     6001 tgcttaacac aagtgcagca taagaggctc ttggggttga agtgctgttc atatagctgt
     6061 ttttatttgg caggtttggt aaaatccatt ctaccacctt caggatattt attagaatat
     6121 ctaactaaat accttccacc attagttatt atactacttt gtggaatagg actatatgta
     6181 ttgcaaccct caaatttttt tccatttatt tcatactcaa aatctatatt agattggcca
     6241 taggttttgc aaaagccttt ggttgtgcca ataatatatt gcggattttt gagtaatgct
     6301 gcttctattt tatatgcatt aaaaacaaaa taaaatattg ttgaaataat aagtaaaaga
     6361 actatgtgaa gtagtttttt attgcgggtt gggtttctaa aaatcctaaa aatatttaaa
     6421 acaatacctc cacttagtat tattagtaaa atagatttta ctatttgatt agtcattaat
     6481 tgctcatcac taacaatgct taaaaacatg actatgtatt tttgggcctc tgtagagacc
     6541 aaggtgtttt atggctacga accttatttt tatcccaata ccacataact tgttgataag
     6601 gcctccatat ataatttaat ggcaacacta ataaatgtat taatctcgaa aaaggtaaaa
     6661 tacctattat aataaaagca ccagcaatat gtaacttaac cattaaaggc aacaatacta
     6721 ttgcagaagt ttcggggtta aatttaaaaa ttgaatatag ataaggagtt agtaaagtag
     6781 aaaaccacga tgagccccag cggtaattat aagcaatcca caagcctgca aatgtttgta
     6841 gaattaataa aacataaatg gcaaaatcca tgtaatttgt tacatacctt aaacgtggtg
     6901 tagtatatct tcttataaat aaattaataa gcccaactaa catacttata ccaaacataa
     6961 aagcagatat ttctaaaatc attaacctaa caggttggct gttccaggct aaaacacctc
     7021 ttggaaaaag aaatgcaatt aagtgcccta aaaaaataaa tacaatgccc cagtgaaaag
     7081 gaactgagcc atagaataac tttttacctt ctaaaaattg agaagataaa gaagagactt
     7141 gaaactttct actagtatat ctatagatag atccaattaa aaaagttgct atagccacat
     7201 aaggcaatac aactaaaatg aaattatcaa aaaggttcat agcctgtagt tttattattt
     7261 ataaaatgtt ttaatttatt tttctgtgag catttctgtt tcaatgttta cactaaaatc
     7321 ttttatttct gttggctcat ccattaacga accagtgctg ccgtttgtat caataatatt
     7381 atatccaaag tctctttgta aaaccata
//
LOCUS       DAOQHP010000066         7344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5300_length_7344_cov_4.089038, whole genome shotgun sequence.
ACCESSION   DAOQHP010000066 DAOQHP010000000
VERSION     DAOQHP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_7344_cov_4.089038"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..905)
                     /locus_tag="PLY81_03375"
     CDS             complement(129..905)
                     /locus_tag="PLY81_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNF29300.1"
                     /translation="MIKKDLIFDIGMHIGQDTRHYLNKGFNVLAIEANPELVQQNKLK
                     FKKEIDEGRLTILNIGISDKNETIPFYKNHRLSEWSSFVKEIGTRLNSSYEIIQVECT
                     TTEDLFKKYGVPYYLKIDIEGNDYLCINAISKESIPSYASCEATSIELLHTLYAKGYR
                     KFKLINQADNFKPINIKKEKSKLFTAYLFIKCGILLRIQKYLPLKHIYSSSGPFGENT
                     KGKWLTYEETEKLYNQFYRKGENEKALNDISWFDFHATII"
     gene            complement(940..2325)
                     /locus_tag="PLY81_03380"
     CDS             complement(940..2325)
                     /locus_tag="PLY81_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29301.1"
                     /translation="MKFTKLICFIYFLNFTFQTIQAQCNHNLWSGSTFSPQPNTCINK
                     VIPNNFVDTINGVATIKSNLACNNSYYYPQFLLKDSLSCISNNFAIEFRIRNSYNIGG
                     IADYDLYFNLYAKNDTGLLNTSLAIMDTLGRQQFTKASFGAIEVTNSPSLLRNLSYWR
                     VVKIHYNNNLAVFSIDGIIMETIPYVSFMKRIDSLSIGFKGAGQVDWIRILNNDTEIW
                     REDFNSVCFSKFPNLCIYNSICDDICNWNLKGNNILLGQNSVPINIFGTLNNSDIRIL
                     TNNTDRGVITANGWLGWNTIPSSLFHIKSSRLFNNPSAIRFQNLPIRDGYILVTDSDG
                     YIYRSNSYSESLYNKIEELEQKVNLLVKQINALKEQNNKGYIKQNVPNPVISNSKTII
                     EYGIEKANGPCYLLITDLTGRQVNKIQLNASLKLSQIELNNNQLNAGLYLYSLIIAGE
                     IIDTKKMIISK"
     gene            complement(2503..3243)
                     /locus_tag="PLY81_03385"
     CDS             complement(2503..3243)
                     /locus_tag="PLY81_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF29302.1"
                     /translation="MLVYFLQEKFIFKPEKLKADFKFKYDIPFTELFFNIEQGVCING
                     LHFYRTNPSGLILYFHGNTRSIKGWAKYARDFYRFNYDVVLVDYRGFGKSTGKRSEKE
                     MLADMQFIYDELKKKYPEHHLLVYGRSMGSGFATKIACDNKPRYLILDSPYYSFKKVV
                     ERFIPILPVKYILRYHLRTDKWIRKVNCHTYIIHGTKDYLIPISQSIKLQKLNPSKVT
                     LIKINGGGHNNLPNFDEYHNFIRDILKE"
     gene            complement(3358..5349)
                     /locus_tag="PLY81_03390"
     CDS             complement(3358..5349)
                     /locus_tag="PLY81_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF29303.1"
                     /translation="MSKNLNKASATGFIIALGIIYGDIGTSPLYVFNAIISNKVVSEE
                     LIIGTLSCIIWTLTLLITIKYVFLVLKADNNGEGGIFSLYALVRRRKKWLIVPAMIGG
                     ATMLADGIITPPITVTPAIEGLKELPALREISTNTIVLIVLGILSIFFIMQQFGTASI
                     GKMFGPFMMIWFTMLAVLGIMHISDDFSILKAFNPMYAIKFLTHYPSGFWLLGAVFLC
                     TTGAEALYSDLGHCGKENIYVSWGYVKLCLVLNYLGQGAFLLSHYKGIEVNAATKASM
                     EINAFFDLMPQWFIIPGVIIATLAAIIASQAMISGSFTLISEAIRLNLWPKFKIRYPS
                     EERGQLFIPSVNLIMFVGCVTMVLHFRESSKMEAAYGLAIVLTMLSTTILFANYLVNK
                     RVQNSWIYLYLAIYIIIEASFLTALLQKFTHGGYITILLGGLMFAVMYVWFKSRKIKN
                     RYVEFVRLEDYISQIQELSNDKAIPKYATHLVYMTSSVHHKEIEHKIIYSILNKKPKR
                     ADIYWFVHVDVLDDPYTCEYIVEHIVPNDIIRIEFRLGFRMEQRINLMFKKVVTDLVA
                     NKEVNINSRYESLGKNNVVGDFQFIVLEKYLSQDNELPFFERIVMKLYFWLKEISLSE
                     ERGFGLDPGFVTVEKFPLIVAPVRNLNLKRVIPEQFDEE"
     gene            5510..5896
                     /locus_tag="PLY81_03395"
     CDS             5510..5896
                     /locus_tag="PLY81_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF29304.1"
                     /translation="MSKQIINTTNAPAPIGPYNQAVKTGNLLFVSGQIPINPATNELV
                     QGTIKEEATQVMLNLKAILSEANLSFEHIVKTTIFLSDMSLFAEVNEIYGSFFMKDFP
                     ARETVAVKGLPKGVNVEISVIATTVL"
     gene            complement(5958..6365)
                     /locus_tag="PLY81_03400"
     CDS             complement(5958..6365)
                     /locus_tag="PLY81_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNF29305.1"
                     /translation="MQNQCSLKIFIDDNPKPIGEYAAPVSFEIDTSKLVDGEHYLRIV
                     SISNSGKEGIRTIPFIVRNGPAISIEGINENDVVDGVVPLMINAYSKGDQKKFLIEGS
                     ETPRSIPAWLWAIIILFVGWSMYYFITSALMKL"
     gene            complement(6374..6634)
                     /locus_tag="PLY81_03405"
     CDS             complement(6374..6634)
                     /locus_tag="PLY81_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29306.1"
                     /translation="MKKISFLLLLALLSAPAVYACPVCERNKPKILQGITPHGSMPNS
                     NWDYVIVIIAVLLLLISLFFSVKWLISPKEGNSDHIKFSIIK"
     gene            complement(6631..>7344)
                     /locus_tag="PLY81_03410"
     CDS             complement(6631..>7344)
                     /locus_tag="PLY81_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNF29307.1"
                     /translation="TATLMGTERTGPDLTDVGNRQPSKEWNLLHLYNPRSVVAQSIMP
                     SYKWLFEIKENPSKNDVIVNVPAEFMHGKTGKVVATKKALQLVAYLQSLKQTSLPESI
                     KPYEFLYKQEKKVTANAGASEEETVDGKALFTANCQSCHQANGEGLKGAFPSLKGSPI
                     VLGDDLKLIVDIIMNGYDARPEFAVMTPVGTSMNFDEKQVAAIINYIKTSWGNDAKKT
                     KADEVKEIIEFIKTNNPAK"
BASE COUNT         2493 a         1177 c          954 g         2720 t
ORIGIN      
        1 ttcttatcca tttatctgtt cttaaatgat accttaaaat gtaattaaca ggcaatattg
       61 gaataaatct ttctactact ttttcttatg ttaattacaa ataaaattaa agtaaaaagt
      121 aattcgtttt aaattattgt tgcatgaaaa tcgaaccaac ttatatcatt taatgctttt
      181 tcattttctc cttttctgta aaactgatta tatagttttt ctgtttcttc ataagtaagc
      241 catttacctt ttgtgttttc tccaaaagga ccagaagaac tgtatatatg ttttaaaggc
      301 aaatactttt gaattcttaa taaaatacca cacttaataa acaaataagc tgtaaataat
      361 ttactctttt cctttttaat atttattggc ttaaaattgt ctgcctgatt aattaactta
      421 aattttctgt atcctttagc atataatgta tgtagtaatt ctattgaagt agcttcgcat
      481 gatgcatagc tcggaatact ctcttttgaa attgcattta tacataaata atcatttcct
      541 tcaatatcaa tttttaaata ataaggtaca ccatattttt taaataaatc ttcggttgtt
      601 gtacattcaa cttgaataat ttcgtaagat gaatttaatc ttgttcctat ttctttaaca
      661 aaagaactcc attcgcttaa cctgtgattt ttataaaaag gtatagtttc atttttgtca
      721 ctaataccta tatttaatat ggtaagcctg ccttcgtcta tttctttttt aaattttagt
      781 ttattctgct gtacaagttc cgggtttgct tctatagcca acacattaaa acctttattc
      841 aaatagtgtc ttgtatcttg accaatatgc attccaatat caaaaattaa atcctttttt
      901 atcatctctt aattatttaa aaactcaatg ctttacattt tattttgaga ttatcatttt
      961 cttagtatct ataatttcgc ctgcaataat aagagaatat aaatataaac cagcattaag
     1021 ttgattatta tttaactcaa tttgtgagag ctttaaagag gcattaagtt gtattttatt
     1081 tacttgccta cctgttaaat ccgtaattag caaataacat ggaccatttg ctttttcaat
     1141 accatattct ataatcgttt tactatttga aataactgga ttaggtacat tttgctttat
     1201 atatccttta ttattttgtt cttttaaagc attaatttgt tttacaagta agtttacttt
     1261 ttgctctaat tcttcaattt tattatataa tgattctgaa tagctatttg atctataaat
     1321 ataaccatca gaatcagtaa caagtatata tccatctctt attggtaaat tttgaaacct
     1381 tattgctgaa gggttattaa ataacctaga acttttaata tgaaataatg atgaaggaat
     1441 agtattccag cctaaccatc catttgcagt aataactccc ctatctgtat tattggttaa
     1501 aattctaata tctgaattat taagagttcc aaaaatgttt ataggtaccg aattttgccc
     1561 taataatata ttattacctt taagattcca gttgcagata tcatcacata ttgaattgta
     1621 aatacacaag tttggaaatt tcgaaaagca gacagagtta aaatcttcac gccaaatttc
     1681 tgtatcatta ttcaatattc ttatccaatc aacttgtcct gcacctttaa agccaattga
     1741 caaactatct attcttttca taaaactcac ataaggaatt gtttccatta ttatcccatc
     1801 tattgaaaaa acagctaaat tattattata atgaatcttt acaactcgcc aataggataa
     1861 gtttctgagt agtgaaggac tatttgttac ttcaatagca ccgaatgaag ctttagtaaa
     1921 ttgctgccta ccaagtgtat ccattattgc caagcttgta ttaagcaacc ctgtatcatt
     1981 ttttgcataa agattaaagt ataaatcata gtcggctatt cctccaatat tatatgaatt
     2041 ccttattcta aactcaattg caaaattatt tgaaatacag gataggctat cttttaataa
     2101 aaattgtggg taataataac tattattaca agctaaattg ctttttattg ttgcaactcc
     2161 atttattgta tcaacaaaat tatttggaat aactttattg atacaagtgt taggttgtgg
     2221 tgaaaaagtt gaaccactcc ataaattatg gttacattgt gcttgtatgg tttgaaaagt
     2281 aaaatttaaa aaataaataa agcatattag cttagtaaat ttcataatag tttattttaa
     2341 aaatcgaagt aaattttatg ccccaaagag agtcaaatgt atgaacaaaa taaattagta
     2401 caaaaaatca ttacttttgt tatcaatagt aattcttatc taacatcata gaaaacgctt
     2461 attcgttctt gggtgtaaag aagaatctgc taaactaaag tgttattctt ttaaaatatc
     2521 tcttataaaa ttatgatact catcaaaatt tggtaaatta ttatgcccac caccatttat
     2581 ttttattaat gtaacttttg atggatttag tttttgcaat tttatacttt gagaaatagg
     2641 tattaaataa tcttttgtac catgaatgat ataggtatga cagtttactt ttcttatcca
     2701 tttatctgtc cttaaatgat accttaaaat gtatttaaca ggcaatattg gaataaatct
     2761 ttctactact tttttaaaac tataataagg cgaatctaaa attaaataac gtggtttatt
     2821 atcgcaggca atttttgtag caaaaccact acccatactt cttccataca ctaataaatg
     2881 atgttcggga tatttttttt tgagttcgtc ataaataaat tgcatatctg ccaacatttc
     2941 tttttcgctt ctttttcctg tactttttcc aaatcctcta taatccacta atacaacatc
     3001 gtaattgaat ctgtaaaaat ctcttgcata ttttgcccaa ccttttatac ttctggtatt
     3061 gccatgaaaa tataagataa gacctgatgg gttagttcta taaaaatgta gcccatttat
     3121 acatacacct tgttctatat taaaaaaaag ttcggtaaat ggtatatcgt atttaaactt
     3181 aaaatcggct tttaattttt ctggtttaaa tataaatttt tcttgtaaaa aatataccag
     3241 cacagataac actattaggc tacaaattat atataatacc cactgattga tagaaaaaaa
     3301 ttatacaata aatatagaaa atatttggtt agattttaag caaatgctta tttatttcta
     3361 ctcttcatca aactgttctg gaataactct ttttaaattt aaatttcgta cgggtgcaac
     3421 aattaatgga aatttttcta ctgttacaaa acctggatct aagccaaagc ctctttcttc
     3481 gcttaatgaa atttctttta accagaaata gagtttcatc acaattcttt caaagaaagg
     3541 taattcatta tcctggctta aatatttttc aagtacaatg aactgaaaat ctccaacaac
     3601 attattcttt cctaagcttt cgtatcggct atttatattt acttctttat tggcaactaa
     3661 atctgtaaca acctttttaa acattaggtt tatacgttgc tccattctaa aacctaacct
     3721 aaattcaatt cttattatat cgtttggtac tatatgttct acaatatatt cacaggtata
     3781 aggatcgtct aaaacatcta catgtacaaa ccaatatata tccgctcgtt taggtttttt
     3841 attaagtatg gaataaataa ttttatgttc aatttcttta tgatgaacag aactggtcat
     3901 gtacactaag tgggtagcat attttggaat agctttatcg ttgcttagtt cttgtatttg
     3961 agaaatataa tcttccagtc taacaaactc aacatagcgg ttttttattt ttctgctttt
     4021 aaaccaaaca tacataacgg caaacattaa accacctagt agtattgtaa tataaccacc
     4081 atgagtaaat ttttgtaata aagcagttaa gaaagaagct tctataataa tatagattgc
     4141 taaatacaaa taaatccaac tgttttgaac tcttttgttt actaaataat tagcaaataa
     4201 aatggttgta gaaagcatgg ttaatacaat tgctaaacca taagctgctt ccattttaga
     4261 agattctcta aagtgtaata ccatagtaac acagccaaca aacataataa ggtttacgct
     4321 tggtataaaa agttgtcctc tttcttcaga tgggtatcga attttaaact taggccaaag
     4381 atttaatcta atagcttcac ttattaaagt aaatgagcca gaaatcattg cctggcttgc
     4441 aataatggct gcaagtgtag caattattac acctggaata ataaaccatt gaggcattaa
     4501 atcaaaaaat gcatttattt ccatagaggc tttagtagca gcatttactt ctataccttt
     4561 ataatgagat aataaaaatg ccccttgccc taaataattt aatactaaac aaagttttac
     4621 atagccccag gatacataaa tattttcttt gccacaatgt cctaaatcgc tgtatagggc
     4681 ttcggcacct gtggtacata aaaatacagc acccaataac caaaaaccac ttggataatg
     4741 tgtaagaaat tttatagcat acattgggtt aaatgctttt agaatactaa aatcatctga
     4801 tatatgcatt attcccaaaa ctgccaacat ggtaaaccat atcatcataa atggtccaaa
     4861 cattttacca atacttgcag taccaaattg ttgcataata aaaaatatcg ataaaattcc
     4921 aagtactatt aatacaatag tattggttga tatttctcta agggctggta attctttaag
     4981 tccttctatg gcaggagtta ctgttatagg tggtgtgata ataccatctg caagcatggt
     5041 tgcaccacca atcattgcag gtacaattaa ccattttttt cgccttctta ctaaagcata
     5101 taaagaaaaa atacctcctt cgccattatt atctgctttt aaaacaagaa aaacatattt
     5161 gattgtaata agtagtgtta gtgtccaaat tatacaagat agtgtaccaa taatcaactc
     5221 ttctgaaact actttgttgc ttataatagc attaaaaaca tagagaggag aagtaccaat
     5281 atcgccataa ataattccta aagcaataat aaaacctgta gcggaggctt tgtttaaatt
     5341 ttttgacaca aatctttttt aaatgataga acaaatgtat aatttataaa aatatacaag
     5401 aactattttt aatttatttc tttaatagga tgatattttt ttattttctg cattaggctt
     5461 tgtggtttta caaaaaatga tttacttgcc cctctataaa tgtaaacgta tgtcaaaaca
     5521 aattattaat acaaccaatg cacctgcacc tattggccct tataaccaag cagtaaaaac
     5581 tggtaacttg ctatttgtaa gtggtcaaat tcctattaat cctgccacta atgaattggt
     5641 gcaaggaaca attaaagaag aagctacaca ggttatgttg aacttaaaag ccattttatc
     5701 tgaagccaac ttaagctttg agcatattgt gaaaacaact atttttctta gcgatatgag
     5761 tttatttgct gaagtaaatg aaatatatgg tagctttttt atgaaagatt ttccagcaag
     5821 ggaaactgtt gcagttaaag ggttacccaa aggagtgaat gtagaaatta gtgtaatagc
     5881 tactacagtt ctttaaaaaa aattgctgcc aacatcatct cataggtgtt atattgatga
     5941 aggcagcaat gaatatatta tagcttcatt aaagcagagg taataaagta atacatagac
     6001 catccaacaa ataggataat tattgcccac aaccatgcag gaatactacg tggggtttca
     6061 cttccttcta ttaagaattt tttctgatct cctttgctgt aggcatttat cattaaaggt
     6121 acaacaccat ctactacatc attttcatta atgccttcaa tagatattgc aggtccattt
     6181 cttactataa atggtatggt tctaatgcct tctttgcctg agttacttat acttacaatt
     6241 cttaaataat gctctccatc cactaattta cttgtatcta tttcaaaaga tacaggagct
     6301 gcatattcac caattggttt tggattatcg tctataaata tttttaatga gcactgattt
     6361 tgcataatta tttttattta ataattgaaa atttaatatg gtcagaattt ccttctttag
     6421 ggcttataag ccatttaaca gaaaaaaata aacttatcaa taaaagtaaa acagctatta
     6481 ttacaattac ataatcccaa ttgctgtttg gcatactgcc atgtggtgta atgccttgta
     6541 aaatttttgg tttatttctt tcgcacactg gacaagcata tacagcaggt gcagaaagta
     6601 aagccaataa taggagaaag cttatttttt tcattttgca ggattattgg ttttaataaa
     6661 ttcaattatt tctttcacct catctgcttt tgttttttta gcatcgttgc cccaactggt
     6721 tttaatatag ttaataattg cagctacttg tttttcatca aagttcatac ttgttccaac
     6781 tggtgtcatt actgcaaatt caggacgagc atcataacca ttcataataa tatctacaat
     6841 tagtttcaaa tcatctccca atacaattgg gctacctttt aatgaaggaa atgcaccttt
     6901 taaaccctcg ccatttgcct gatgacagct ttggcagtta gcagtaaata atgctttacc
     6961 atcaactgtt tcttcttctg aagcacctgc attggcagtt actttctttt cttgcttgta
     7021 taaaaactca taaggtttta tactttctgg taatgaggtt tgttttaatg attgtaaata
     7081 agcaactaat tgtaaagctt ttttggttgc aaccactttg cctgttttac catgcataaa
     7141 ctctgcaggt acgtttacta taacatcatt tttagaagga ttttctttta tttcgaaaag
     7201 ccatttataa cttggcatta ttgattgtgc aacaacacta cgagggttat aaaggtgtaa
     7261 taaattccac tctttacttg gttgtctatt acctacatct gttaaatctg gtcctgttct
     7321 ttccgtgccc attaaagtgg cagt
//
LOCUS       DAOQHP010000067         7340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5305_length_7340_cov_3.468360, whole genome shotgun sequence.
ACCESSION   DAOQHP010000067 DAOQHP010000000
VERSION     DAOQHP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_7340_cov_3.468360"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLY81_03415"
     CDS             complement(<1..230)
                     /locus_tag="PLY81_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF29308.1"
                     /translation="MSKKRILFIATEMSPYLELTEFAEIVNKLAIKSNESGMEVRCIM
                     PRFGVINERRHRLHEVVRLSGINVSVNDDDYP"
     gene            complement(409..2046)
                     /gene="groL"
                     /locus_tag="PLY81_03420"
     CDS             complement(409..2046)
                     /gene="groL"
                     /locus_tag="PLY81_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNF29309.1"
                     /translation="MAKQIFFDIEARNKMKKGVDTLSNAVKVTLGPKGRNVVIDKKFG
                     APQITKDGVTVAKEIELEDAIENMGAQMVKEVASKTADLAGDGTTTATVLAQSIISEG
                     LKMVAAGANPMDLKRGIDKAVSLVVENLKGQSQTVGSDSKKIQQVATISANNDETIGK
                     LIAEAFGKVGKEGVITVEEAKGTDTTVDVVEGMQFDRGYISPYFVTNSEKMEAELQNP
                     YILIYDKKISAMKDILHILEKVAQSGRPLLIIAEDLEGEALATLVVNKLRGTIKVAAV
                     KAPGFGDRRKEMLTDIAILTAGTVISEELGHKLEGADLTSLGQAASVTIDKDNTTIVG
                     GKGKKADITARVNQIKAQVENTTSDYDREKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALAATRAAVEEGIVPGGGVAYIRAIDSLDAKVKGQIADEQTGMAIVRRALEE
                     PIRTLTTNAGIDGSIVVQKIKEGKGDFGFNARTEVYENLFKAGVIDPTKVSRVALENA
                     ASIAGMLLTTEAVIADKPEPKSATPAMPGPDMGGMGY"
     gene            complement(2167..2451)
                     /locus_tag="PLY81_03425"
     CDS             complement(2167..2451)
                     /locus_tag="PLY81_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNF29310.1"
                     /translation="MATKKLNVTPLHDRVIVKPAAAEEKTAGGIIIPDTAKEKPQRGI
                     VIAAGAGKKDEPVTVKVGDNVLYGKYAGTEIQIEGQDLLIMRESDILAIV"
     gene            complement(2654..4315)
                     /locus_tag="PLY81_03430"
     CDS             complement(2654..4315)
                     /locus_tag="PLY81_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNF29311.1"
                     /translation="MSEEKSLNFIEEIIEKDLQDGKHETILTRFPPEPNGYLHIGHAK
                     SICLNFGLALKYNGKTNLRFDDTNPVTEETEYVESIKEDINWLGFKWTNEFYASDYFD
                     KLYEFAIILIKKGLAYVDDSSSEEIAQQKGTPTIPGIRNRFSERDIEENVKLFTEMKD
                     GKYVDGEKVLRAKVDMSSPNMHMRDPIIYRIKHAHHHRTGNKWCIYPMYDFAHGQSDS
                     IENITHSICTLEFIPHRELYNWLIQQLAIFPSQQYEFARLNMTYTVMSKRKLLQLVNE
                     KHVVGWDDPRMPTISGMRRRGYGAAALRDFCDKIGIAKRENLIDIGLLEFCVREELNK
                     TALRRMVVFNPLKITITNFNKEEEMVSIEDNPEDPNSTHRMVPFSKEIYIEQEDFMEI
                     PPKKFFRLYPGGRVRLKGAYIIECNEVVKDNEGNITELQCSYLPESKSGNDTSGIHVK
                     GTLHWVSKAHAVHAEVKLFDRLFHIENVAEAEGDFKDHINPNSLQIIEKVYAEPALLD
                     IDIQKKYQFLRKGYFCIDKDSTKEKLIFNRTVTLKDTWAKEVKKN"
     gene            4433..4723
                     /gene="gatC"
                     /locus_tag="PLY81_03435"
     CDS             4433..4723
                     /gene="gatC"
                     /locus_tag="PLY81_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNF29312.1"
                     /translation="MNVTTELVDKLANLTMLQFNEEEKKDIQNDLQSMISFVEKLQEL
                     DTNGVEPLQHISSNNNVLREDNIKPSISKEEAFLNAPLQHENYFAVSKIIKK"
     gene            complement(4726..5391)
                     /gene="aat"
                     /locus_tag="PLY81_03440"
     CDS             complement(4726..5391)
                     /gene="aat"
                     /locus_tag="PLY81_03440"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNF29313.1"
                     /translation="MAITLLTEKLWFPPVETASLDGLLAIGGDLQPERILLAYQSGIF
                     PWYDGDLPLWWFPNPRFVLLPNELKISKSMQQLIKQNKFKVTVNNAFSEVIKNCKNCI
                     RKDQEGTWINEEVVNAYTQLHKMGYAHSVEAWQNNELVGGLYGIMMGQVFFGESMFSL
                     KSNASKYAFISYVQQLAKEGVALIDCQVYTKHLESLGAKMIMKEQFSQLLKHFLHSTQ
                     INK"
     gene            complement(5498..5572)
                     /locus_tag="PLY81_03445"
     tRNA            complement(5498..5572)
                     /locus_tag="PLY81_03445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5535..5537),aa:Val,seq:tac)
     gene            5718..6020
                     /locus_tag="PLY81_03450"
     CDS             5718..6020
                     /locus_tag="PLY81_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNF29314.1"
                     /translation="MLLHSKTNSNSKEQVDVDVLEISISPFQLIVWNDEVNTFEWVIK
                     SLIEVCNHTEEQAEQCAFLIHFKGKYAVKNGDYDTLKPMCNSITERGINATIEQLN"
     gene            complement(6028..6972)
                     /locus_tag="PLY81_03455"
     CDS             complement(6028..6972)
                     /locus_tag="PLY81_03455"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNF29315.1"
                     /translation="MKNWLFISFFLTFCGLNAQEIVVKRISGDIFKSVKKEADTSKWK
                     WKRNGIVGVNIAQGALSNWAAGGDNFSMSINTNFSYYIYHRAKRYTWDNSFDFNFGLV
                     QATTLGSRKNDDRIDLLSKFGYKVDTLNKWYASMLFNFRSQLFDGYNYSGSSSTFSSS
                     FLSPAYIILSVGMDYKPSPKFSLFVSPLTSRWVIVANDYLSKKGAYGVPINKHSVGEL
                     GAYVTMSYVNEIATNISYRGRMDLFSNYKNNPQNVDIFFTNMFSFRINKLFSATYNLD
                     LIYDDDIKIFGSNKNAPRLQLKSLIGIGFLMRLEPKKI"
     gene            7123..>7340
                     /locus_tag="PLY81_03460"
     CDS             7123..>7340
                     /locus_tag="PLY81_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNF29316.1"
                     /translation="METLTLVDKTHDEARQGEAFAPFKLDNNIYKKYFYIESYGCQMN
                     FSDSEIVASILHAEGFGATKDITIADLI"
BASE COUNT         2357 a         1299 c          962 g         2722 t
ORIGIN      
        1 aaaggataat cgtcatcatt aacagataca ttgataccac ttaatcttac tacttcatgt
       61 aagcgatgcc ttctttcgtt tattacacca aatcgtggca taatacagcg aacttccata
      121 ccactttcat tgctttttat ggctaattta ttcactattt cagcaaattc agtaagctct
      181 aggtatggcg acatttctgt agctataaat aaaattcttt ttttcgacat cagctcttct
      241 aatttagggg gtcgtcttta taaggctgca aaattaagat attatgctct aatatcaaac
      301 gtatttatga tattaacagc cttaaattca tcattttttt taattcttaa tcaaaaaacc
      361 ccgaaatgta aatttttcgg ggctttttgt tgtatctgta attttaaatt aatatcccat
      421 tccacccata tctggacctg gcattgcagg agttgcagat tttggttctg gtttatcagc
      481 aataactgct tcggtagtta ataacatacc tgcaatagat gctgcatttt ctaaagctac
      541 tctacttact ttggtagggt ctattacacc tgctttaaat aagttttcat acacttctgt
      601 tcttgcatta aaaccaaaat ctcctttgcc ttctttaatc ttttgaacta caatagagcc
      661 atcaatacct gcattggtag ttaaagttct aataggttct tctaatgctc ttcttactat
      721 tgccatacca gtttgttcat ctgctatttg tccttttact ttagcatcta aactatcaat
      781 tgctctaata taagcaacac caccacctgg tacaatacct tcttctactg cagctcttgt
      841 tgcagctaat gcatcatcca ctctgtcttt cttttctttc atttctacct ctgtagcagc
      901 accaacgtat aatacagcta caccaccagc taattttgct aaacgttctt gtaatttttc
      961 tctgtcatag tcagaggttg tattttctac ttgtgctttt atttgattta cacgagcagt
     1021 gatatcagct tttttacctt taccgccaac aatagttgta ttgtctttat caattgttac
     1081 tgatgcagct tggcctaatg aagttaaatc tgcaccttct aatttatgtc caagttcttc
     1141 gctaattaca gtacctgctg ttaatattgc aatatctgta agcatttctt ttcttctatc
     1201 accaaatcct ggggctttta ctgctgctac ttttattgta ccacgtaatt tatttacaac
     1261 taaagttgct aatgcttctc cttctaaatc ttctgcaata attaataaag gacgaccact
     1321 ttgagctact ttttctaaga tatgtaaaat atctttcatt gcagaaatct tcttatcata
     1381 aattaaaatg taaggatttt gcaattcagc ttccattttt tcgctattgg ttacaaagta
     1441 tggagaaatg taacctctat caaactgcat accttctaca acatcaacag ttgtatcagt
     1501 accttttgct tcctctacag ttataacacc ttctttacct actttaccaa aagcttcagc
     1561 aattaattta ccaattgttt catcgttgtt ggcagatata gttgctactt gttgaatttt
     1621 tttgctgtcg cttccaactg tttgcgattg gccttttaag ttttcaacaa ctaaagaaac
     1681 tgctttatca atgcctcttt ttaaatccat tgggtttgca ccagcagcta ccattttcaa
     1741 tccttcgcta ataattgatt gtgctaaaac agttgcagtt gtagtaccat cgccagctaa
     1801 atcggcagtt ttagatgcaa cttctttaac catttgtgca cccatgtttt caatagcatc
     1861 ttctaattca atttcttttg ctacggttac accatcttta gttatttgtg gtgctccaaa
     1921 ttttttatca ataactacat tacgaccttt agggccaaga gttactttta cagcgttaga
     1981 taaagtatcc acgccttttt tcattttatt tcttgcttca atatcaaaga aaatttgctt
     2041 tgccatattt cttactgttt aagaatttta tttataatta gtattttttg tttttcaaca
     2101 ccagtacctc tatgtttcag aagttgcgtc gcacacttgt acttttagta ctttgcttat
     2161 cgtattttaa acaatagcta aaatatccga ttctctcata attaaaaggt cttgcccttc
     2221 aatttgaatt tcggtacctg catatttacc atataaaacg ttatcaccaa cctttactgt
     2281 aacaggctca tcttttttac cagcaccagc agctattaca ataccacgtt gaggtttttc
     2341 ttttgcagta tcaggtataa taattcctcc tgcagttttt tcttctgcgg cagcaggttt
     2401 tacaattact ctgtcgtgca atggagtaac atttaatttt tttgtagcca taatcctaaa
     2461 tttttatttt ttgtatttta tttaaaaatg tataacccta tcttcaacaa tattgccatt
     2521 gatggaataa ggtcaaaatt tcagttttaa tttaattagc taaaaaattg gcagcgaaga
     2581 tgcagaattt tagacaaata gccgtgtcaa aatttcacat aactcattaa aataaaatgc
     2641 aaagctgtaa gttttaattt ttctttactt cttttgccca ggtatctttt aaggttacag
     2701 ttcggttaaa aattaatttt tctttggtac tgtccttatc tatacaaaaa tatcctttgc
     2761 gtaaaaattg atactttttt tggatatcaa tatctaataa agcaggctct gcataaacct
     2821 tttctattat ttgtaaggaa ttggggttta tatggtcttt aaaatctccc tctgcctctg
     2881 ctacattttc tatatgaaat aatctatcaa ataatttcac ttctgcatgt acagcatgtg
     2941 ctttactaac ccaatgtaat gtacctttta catgaatacc actcgtatca tttccacttt
     3001 tactttctgg caaataacta cattgtagct ctgtaatatt tccttcattg tcttttacta
     3061 cttcattaca ttcaattata tatgcacctt ttagtcttac tcttccacca ggatataatc
     3121 taaaaaattt ctttggtgga atttccataa aatcctcttg ttctatataa atttctttac
     3181 taaaaggcac catacgatgt gtagaatttg gatcttctgg attatcttct atacttacca
     3241 tttcttcttc tttattaaaa ttggttatag taatttttaa tggattaaat acaaccattc
     3301 ttctaagagc agttttattt aactcttctc ttacacaaaa ttctaacaaa ccaatatcaa
     3361 tcaaattttc tcttttagca attcctattt tatcacaaaa atctcttaaa gcagcagctc
     3421 cataccctct tcttctcata ccactaatag ttggcattct tggatcgtcc catccaacta
     3481 catgtttttc atttactagt tgtaacaatt ttcttttact cattacagta taagtcatat
     3541 ttaaccttgc aaactcatat tgttgcgaag ggaagattgc taactgttga ataagccaat
     3601 tgtatagctc tctatgaggt ataaattcaa gggtgcaaat tgagtgtgta atattttcaa
     3661 tagaatcact ttgtccatga gcaaaatcgt acattggata aatacaccat ttattacctg
     3721 ttctatgatg gtgagcatgc ttaattctgt atataatagg atctctcatg tgcatattgg
     3781 ggctgctcat atctactttt gctcttaata ctttttcacc atcgacgtat ttaccatctt
     3841 tcatttcggt aaataatttt acattttctt ctatatctct ttctgaaaat ctatttctta
     3901 taccaggtat tgttggagta cctttttgct gagcaatttc ttcgctgctg ctatcatcta
     3961 cataagctaa tccttttttt attaaaataa ttgcaaactc atataattta tcaaaataat
     4021 cagatgcata aaattcattt gtccacttaa agcctaacca gttaatatct tctttaatac
     4081 tttctacata ttctgtttct tctgttacag ggtttgtatc atcaaaacgc aagttagttt
     4141 taccattata ttttaatgct agaccaaaat ttaaacaaat acttttggca tggccaatat
     4201 gtaagtaccc attcggttct ggcggaaaac gtgtaagaat agtttcatgc tttccatctt
     4261 gtaaatcttt ttcaataatt tcttcaataa aatttaaact cttttcttca ctcatactat
     4321 ttattttagt agctggcaaa aatacgcttt agataatagt gaaaacaaaa aacaattcaa
     4381 ctaaaaaaaa tcaataaaat taaaaatcaa agaattaatt atttttgtag ttatgaatgt
     4441 tacaactgaa ttagtggata agttagccaa ccttacaatg ttgcaattta atgaggaaga
     4501 aaaaaaagat attcaaaatg atttgcaaag catgatttct tttgttgaaa aactacaaga
     4561 attagataca aatggagttg aaccattgca acacataagc agcaataata atgttttaag
     4621 agaagataat attaaaccaa gtattagtaa agaagaggca tttttaaatg caccattaca
     4681 acatgaaaat tattttgctg tttctaaaat aattaaaaaa taaggttact tatttatttg
     4741 tgtactgtgt agaaaatgtt taagcaattg cgaaaactgt tctttcataa tcatttttgc
     4801 tcctaaactt tctaaatgct ttgtatatac ctggcaatct attaaagcta caccttcttt
     4861 tgcaagttgc tgtacataac ttataaatgc atatttactg gcattagatt ttaagctaaa
     4921 catactttca ccaaaaaaaa cttgccccat cattatgcca tataaccctc ctactaactc
     4981 attattttgc caagcttcta ccgaatgtgc atagcccatt ttatgtaatt gagtataagc
     5041 atttaccact tcttcattta tccaagtacc ttcttgatct tttctaatac aatttttaca
     5101 gtttttaatt acttcagaaa atgcattgtt tacagtaacc ttaaatttat tttgcttaat
     5161 taattgctgc atgcttttac tgatttttag ctcattgggt aataaaacaa atcttgggtt
     5221 aggaaaccac caaagtggta aatcaccatc gtaccaagga aaaataccag attgataagc
     5281 aagtaaaatc ctttcgggct gtaaatctcc gccaattgct aataatccat ctaaagaagc
     5341 tgtttctacc ggaggaaacc aaagtttttc tgtaagtaat gttatagcca aatgtaatta
     5401 ttaaactagt tgatcaaata ggaaagataa aatcagaaag ctaacaacaa agaaaattta
     5461 tttcataaaa aaaacctaca aaatttgtag gtttttgtgg gagctgacgg gttcgaaccg
     5521 ccgaccctct gcttgtaagg cagatgctct aaaccagctg agctaagctc ccatattttt
     5581 aatttgggat tgcaaaaata ggtgcaataa aatattagcc aaaaaaaatt tcagttttta
     5641 aaaaatattt cttcttttac ttaatattat tttatttgtt gctaatttgt agttttattt
     5701 tttaaaacca attatgcatg ttgctgcatt caaaaacaaa cagtaattca aaagagcagg
     5761 tagatgtgga tgtacttgaa ataagtattt caccttttca attaattgtt tggaatgatg
     5821 aagtaaatac ttttgaatgg gtaataaaat ctttaattga agtttgtaac catacagaag
     5881 agcaagctga acaatgtgct tttttaattc attttaaagg aaaatatgca gtaaaaaatg
     5941 gcgattatga tacgttaaag cctatgtgta actctattac tgaaagagga attaatgcca
     6001 ccatagagca gttaaattaa gcctatttta gattttttta ggctccagcc tcattaaaaa
     6061 gccaatacca attaaacttt tcaattgtaa acgtggagca tttttattag aaccaaaaat
     6121 tttaatatca tcatcataga ttaaatccaa attataggtt gctgaaaata atttattgat
     6181 tctaaaagag aacatattag taaaaaagat atctacattt tgaggattat ttttatagtt
     6241 agaaaataga tccattctac ctctatatga aatattggtt gcaatttcgt tgacatagct
     6301 catggttaca taagccccca attcaccaac agaatgtttg tttataggca caccataagc
     6361 accttttttg gataaataat catttgcaac aataacccat cttgacgtta atggtgaaac
     6421 aaaaagtgag aattttggag aaggtttgta atccatacca acagataaga taatatatgc
     6481 aggcgataaa aaggaagatg aaaaagttga actactacct gaatagttgt agccatcaaa
     6541 caattggctt ctaaagttaa ataacataga agcataccat ttatttaaag tatctacttt
     6601 atatccaaat tttgaaagta agtcaattct atcatcattt tttctacttc ctaaagtagt
     6661 agcttgaact aagccaaaat taaaatcaaa actattatcc catgtatatc ttttagctct
     6721 atgataaata taataactaa aatttgtatt tattgacatc gaaaagttat cgccacctgc
     6781 agcccaattg cttaatgctc cttgtgcaat attaactcca acaataccat ttcgtttcca
     6841 tttccattta cttgtatcag cttctttttt tacactttta aatatatctc cagatattct
     6901 tttaactaca atttcttgag catttaggcc acaaaatgtt aaaaaaaatg aaataaataa
     6961 ccaatttttc atgtatttgc aacaaggtct tttcatgaat gaatgtaaaa atatttttta
     7021 caaaaataat atcagccaat ttaaattctg ttaatagtct gtcaaattgg caatagtaat
     7081 attttttgct acttttgcag cttaaataag tatttaattt taatggaaac tttaacgcta
     7141 gtagataaaa cacatgatga agcaagacaa ggggaagctt ttgcaccttt taaacttgat
     7201 aataacattt acaaaaaata tttttatatt gaaagctatg gctgccaaat gaactttagt
     7261 gatagtgaaa ttgtagcttc tattttacat gcagaaggtt ttggtgccac aaaagatatt
     7321 acaatagccg acttaatatt
//
LOCUS       DAOQHP010000068         7303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5346_length_7303_cov_3.632726, whole genome shotgun sequence.
ACCESSION   DAOQHP010000068 DAOQHP010000000
VERSION     DAOQHP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_7303_cov_3.632726"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /gene="thrS"
                     /locus_tag="PLY81_03465"
     CDS             complement(<1..1903)
                     /gene="thrS"
                     /locus_tag="PLY81_03465"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNF29317.1"
                     /translation="MINISFPDGAVRQYEAGVSAMDIAKSISEGLAKKVLVAGVNNEV
                     WDASRPITTDSSLKLFTWNDAEGKNTFWHSSAHLMAEAIEAKFPGVKFWVGPALENGF
                     YYDIDLDDRKITEEDLVELEKIMNELAKKNNVYLRTEKAKQEAIAYFTEKGDEYKLDL
                     LQNLEDGTITFYTQGNFTDLCRGPHIPNTGFIKAIKLLNIAGAYWKGDEKNKQLTRIY
                     GITFPTQKELDEYLTMLEEAKKRDHRKLGKELGIYTMNDDIGQGLILWMPNGTIIIEE
                     LEKLAKQSEEAAGYKRVVTPHIAKENLYLTSGHLPYYADSMFAPMEMDGEKYYLRAMN
                     CPHHHKIFDAEPKSYRDMPYRIAEYGTCYRYEQSGELFGLMRVRCLHMNDAHIYCTKE
                     QFAQEFKAVNDLYLKYFKIFGIEKYVMRLSLHDPEKLGQKYVNEPELWLETEELVRNV
                     LKETGVPFVEVKGEGAFYGPKIDVQIWSAIGREFTLATNQVDFAQPRSFKLSFTNQNN
                     EPEIPLIIHRAPLGTHERFIGFLLEHYAGKFPVWLAPLQVKILPISDKFLPYATTVKN
                     ALQQVGVRVEIDDRNEKIGKKIREAELARVPYMLVVGEKEATENKLSVRRQGKGDVGQ
                     LTIEEFTKSI"
     gene            complement(2058..3554)
                     /gene="guaB"
                     /locus_tag="PLY81_03470"
     CDS             complement(2058..3554)
                     /gene="guaB"
                     /locus_tag="PLY81_03470"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNF29318.1"
                     /translation="MATIKPSLSKNKVNRVFIDGLTFDDVLLLPAYSQVLPREVNLHS
                     HLTKEIVLNIPLLSAAMDTVTEANLAIALAREGGLGILHKNMSIEKQAEQVRKVKRSE
                     SGMIIDPLTLTTNSTIGDALKLMKENKIGGIPIIDNQGKLVGILTNRDLRFETDKKRK
                     VTDVMTNEKLITAPEGTDLKKAEIILRKYKIEKLPVVNKQGKLVGLITYRDILQLTNH
                     PNAVKDNYGRLLVGAALGITNDLMQRADALMNVGVDIVTLDSAHGHSKGVIEALKALR
                     KNFKKLQIIAGNVGTAEGALALANAGADCVKVGIGPGSICTTRIVAGAGVPQLTAVME
                     ASNALKKKNIPVIADGGIRYTGDMVKALAAGANTVMMGSVFAGTEESPGETIIYEGRK
                     FKTYRGMGSLGAMSQGSGDRYFQDVEDDVKKFVPEGIEGRVAFKGNLSEIVYQFVGGL
                     RSGMGYCGAKNIAALQNATFVKITNAGMKESHAHDIDITKEAPNYSRK"
     gene            complement(3650..4003)
                     /locus_tag="PLY81_03475"
                     /pseudo
     CDS             complement(3650..4003)
                     /locus_tag="PLY81_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
     gene            complement(4016..4192)
                     /locus_tag="PLY81_03480"
     CDS             complement(4016..4192)
                     /locus_tag="PLY81_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29319.1"
                     /translation="MLLLLQNFSEIEKELFLKNESEMQDNFLSMLHYFSKANTNNDFP
                     LLRSGLVLSGVNNY"
     gene            4368..5552
                     /locus_tag="PLY81_03485"
     CDS             4368..5552
                     /locus_tag="PLY81_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29320.1"
                     /translation="MKLFTVFLVILFVSCNGQSVLEVSKENSNTVGGGCEGCELMYVE
                     IPDEISNEHTSLGWFEGKQKLIITGTVFQLDGKTPAANTLIYYWHTDDAGLYSSNEQT
                     SEKVKAHGKLRGWIKTDSSGKYTIKTSRPASYPNEDIPQHIHLSIKEPNITNEYFADL
                     YFDDDKLYLAHKKKYGKIDRAGSEILRVLIDNEVQIAEHDIILGLNIPHYPSLKQSNN
                     LSGLNIGEDQPSFTPYHAFGADKGTQTCPVCKYGRYHGIIYFVGNHPNWNEIKTWLIF
                     LDNESRLREKYLKTYFVLGNEKEFIHENTFTFLESLGKELNLQNIALTFVPSFNDSST
                     EVNLNKIDAEAKNTIIIYKHRTIVDKYINLLPTKENFAKIINVLDKTKGNYFNLEEPK
                     HD"
     gene            5564..5986
                     /locus_tag="PLY81_03490"
     CDS             5564..5986
                     /locus_tag="PLY81_03490"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF29321.1"
                     /translation="MSELKTKKNNASVIDFINLVEKEQKRKECFQLLELFETVTKEKA
                     SMWGSSIIGFGEYKYKYESGREGDWFFAGFSPRKQNIVIYIIAGFTDYESLMQQLGKY
                     KTGSSCLYINKLADIDLKVLKLLIEKSFAFMKNKYAKN"
     gene            complement(6011..7276)
                     /locus_tag="PLY81_03495"
     CDS             complement(6011..7276)
                     /locus_tag="PLY81_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNF29322.1"
                     /translation="MKFLNLLFVLFTANFLYAQNKVVDTNQVVTPNRVNTEQTLQKPY
                     VILISADGFRYDYFKKYKAKNLLNIAAKGVWAKKGMHPSYPSITFPNHYSIITGLYPS
                     HHGIVDNIFYDSIRKETYKIGSKTVIDGTWYNGLPLWALAEKQGMLSASLFWVGSESN
                     AANTRPTYWYHYHEKFKGDAKVNIIKNWLTLPDSIRPHFITLYFPEVDYYGHKFGPDA
                     KETEASVHYIDVAIKKLVDSLQPLNLPINFIFVSDHGMIAVEEKNYIPLPEIDSKKFT
                     IINSYTLVRVTAKDKLDVLPLYQKLLNEKSDDYNVYLANNFPEKLNYSSKEDTIGRIG
                     DILLVPVGSKLLVNKNKKPTYGKHGFNSYEIPEMKASFIAWGENFKKGKKIKTFQNIH
                     IYPLVASILDLEILQPIDGSLKVLQKTIR"
BASE COUNT         2376 a         1264 c         1032 g         2631 t
ORIGIN      
        1 caatgctctt agtaaactct tctattgtta attggcctac atctcctttt ccttgcctac
       61 gaacagagag tttattttct gtagcttctt tttcgccaac aacaagcata tagggtacac
      121 gggctagttc tgcttctcta atttttttac ctattttctc atttctatcg tctatttcta
      181 cccttacacc aacttgttgc agtgcatttt taactgttgt ggcataaggt aaaaacttat
      241 cgctgatagg taaaattttt acttgtaatg gtgctaacca aacaggaaat ttaccagcat
      301 aatgttctaa caaaaagcca ataaatcttt catgtgtgcc taaaggtgct ctatgaataa
      361 ttaaaggaat ttctggttcg ttattttggt tagtaaaaga taatttaaaa ctgcgaggtt
      421 gagcaaaatc aacctggtta gttgctaaag taaactctct accaatggca ctccatattt
      481 gaacatctat tttagggcca taaaatgcac cttctccttt tacttcaaca aaaggtacac
      541 ctgtttcttt taaaacattt cttaccaatt cttctgtttc aagccaaagt tctggctcgt
      601 ttacatattt ctgtccaagt ttttcaggat cgtgtaagct taacctcatt acatattttt
      661 caataccaaa aattttgaaa tattttaggt acaaatcgtt tactgcctta aactcttgtg
      721 caaattgttc tttagtacaa taaatatggg catcattcat gtgcaggcat ctaaccctca
      781 ttaatccaaa taattcacca ctttgctcgt atctgtaaca agtaccatat tctgcaatac
      841 gataaggcat atctctataa ctcttaggct ctgcatcaaa tattttatgg tggtgtgggc
      901 agttcattgc tcttaaataa tatttttcac catccatttc cattggggca aacatactat
      961 cagcataata gggcaaatga ccgcttgtta agtacaaatt ttcttttgca atatgtggtg
     1021 taacaactct tttatagcct gctgcctctt cagattgttt tgctaatttt tctaattctt
     1081 caataataat agttccattg ggcatccata aaattaaccc ttggccaatg tcatcattca
     1141 tagtataaat tcctagttct ttacctaatt ttctatgatc tctttttttg gcttcttcta
     1201 acattgttag atattcatct aactcttttt gtgtaggaaa agtaatacca taaattcggg
     1261 taagctgttt atttttttca tctcctttcc aataagctcc tgcaatattt aatagtttta
     1321 ttgctttaat aaaacctgta ttaggaatat gtgggccacg acataagtca gtaaaattac
     1381 cttgtgtata aaaagttata gttccatctt ctaagttttg aagaaggtct aatttgtatt
     1441 catcgccttt ttctgtaaag taagctatcg cctcttgttt ggctttttct gttcttagat
     1501 atacattatt tttttttgct aattcgttca ttattttttc caactccact aaatcttctt
     1561 ctgtaatttt tctatcgtct aaatcaatat cataataaaa tccattttct aaagctggac
     1621 caacccaaaa tttaacgcca ggaaattttg cttctattgc ttctgccata aggtgtgcag
     1681 aagagtgcca gaatgtattt ttcccttcgg catcgttcca agtaaaaagt tttaaagaac
     1741 tatctgtcgt aataggtctt gatgcatccc acacctcatt atttacacca gctaccaata
     1801 ctttttttgc caacccttcg ctaatacttt ttgcaatatc cattgcactt actccagctt
     1861 cgtattgcct tacagcacca tcaggaaaac taatatttat catactataa acaactaaaa
     1921 tttttatggg tgcaaaagta acaaatgaaa ggcattaaga aggataaatt atgaactttg
     1981 tactataaac aaataaatat gcttttaagt gatgctttaa attggattat ttgcgtttat
     2041 aaatgataat ttctgattta ctttctgcta taattaggag cttctttggt aatatcaata
     2101 tcgtgagcat ggctttcttt cattcctgca ttagtaattt ttacaaatgt agcattttgc
     2161 aaggctgcaa tattttttgc tccacaataa cccataccag agcgtagccc tcctacaaat
     2221 tgatacacta tttcgcttaa gttgccttta aaagcaactc taccttcaat gccttctggt
     2281 acaaattttt tcacatcatc ttcaacatct tgaaaatatc tatcgccact gccttgactc
     2341 atagctccta aacttcccat gcctctatat gttttgaact ttctaccttc ataaattata
     2401 gtttcgccag ggctttcttc tgtaccagca aatacactgc ccatcattac agtatttgca
     2461 ccagcagcta aagcttttac catatctcct gtataacgaa taccaccatc tgcaataaca
     2521 ggaatatttt ttttcttcaa agcattactt gcttccataa ctgctgtaag ttgtggaacc
     2581 cctgcacctg caactatacg agttgtgcaa atactacctg gtccaatacc tacttttaca
     2641 caatctgcac ctgcatttgc caaagccaaa gctccttctg ctgtgcctac atttcctgca
     2701 ataatttgta gttttttaaa attttttctt aatgctttta aggcttctat tactcctttg
     2761 ctgtgcccat gagcactgtc taaggtaaca atatcaactc ctacattcat taaagcatcg
     2821 gcacgttgca ttaaatcgtt tgtgatgcct aatgctgcac caaccaataa acgaccatag
     2881 ttatctttta ctgcattagg gtgattggta agttgcaaaa tatctcgata cgtaattagc
     2941 ccaactaatt tgccttgttt gtttactact ggtagttttt caattttata ttttcttaaa
     3001 attatttctg ccttttttaa atctgttcct tcgggggctg taattaactt ttcgttagtc
     3061 attacatcgg taacttttct ttttttatct gtttcaaaac gaaggtctct gttagtaaga
     3121 attccaacaa gtttaccttg attatctata attggaattc ctccaatttt attttctttc
     3181 attaacttca aagcatcgcc aatagtagaa ttggttgtta aggttaatgg gtctataatc
     3241 attccacttt cacttctttt aacttttcta acttgttctg cctgtttttc aatgctcata
     3301 tttttgtgca gaatgcccaa acctccttct cttgctaaag caatagcaag attagcttct
     3361 gtaactgtat ccattgctgc cgaaagcaaa ggaatattta atactatttc tttagtaagg
     3421 tgagaatgta aattaacttc tcttggtaaa acctggctat atgcaggtaa taaaagtaca
     3481 tcatcaaaag ttaaaccatc aataaaaaca cgattaactt tattttttga aagtgaaggt
     3541 tttattgttg ccatgtgcaa aattaacggc tcaaaagata tgtaacaaat gaagtttgga
     3601 tttaattaaa aaactgactt tttagatttt aaaactttct ttgacaatat tactctccta
     3661 ctaatacaaa tccagcccat ttagttgggt ctttgtattt ggcttttaat gttaattgtg
     3721 cttttctaaa agctgaataa taagaatctc ctttaaaaaa attactataa aaaagttgca
     3781 tcaattctgc agtttcagta tcaggaactt cccataagct caagagtaat ttatttaccc
     3841 cagccatttt aaatgcacgc tacaaaccat acacaccttc gcttccatta atatctccta
     3901 aagctgtttg gcatgcactc aataccacta aatcggtatt taataaattg aggttgctta
     3961 tttcctgtgc agtaacaatg ccatcttctt tgttttctaa aatagtatct ttccctcagt
     4021 aattatttac tccacttaaa accaagccac ttcttagtaa tggaaaatca ttatttgtat
     4081 tagcttttga aaaataatgt aacatagata gaaaattatc ctgcatttcg ctttcatttt
     4141 ttaaaaacag ttctttttct atttcactaa aattttgtag aagcaacagc aatttaatta
     4201 tagttttaac tgaatgaatt gcagaaaaat gtataactca aactttatcg tagaaatgaa
     4261 atttaatatt aaattacttt atgtatttag ttagtttaac tatttttact aatagctcaa
     4321 ctaataatac gattacaaag ttcccaaaca aatataaaat gaattgtatg aaactcttta
     4381 ctgtatttct agttattcta tttgtaagct gtaatggtca atcagtttta gaagttagta
     4441 aagaaaatag caatacagtt ggtggtggtt gcgaaggttg tgagttaatg tatgtagaaa
     4501 taccagatga aattagcaat gaacatacca gcttgggatg gtttgagggc aaacaaaaat
     4561 taattattac aggtacagta tttcaacttg atggtaaaac cccagctgcc aatactttaa
     4621 tttattattg gcatacagat gatgctggct tgtattcaag caatgaacaa acttcagaga
     4681 aagtaaaagc acatggcaaa ttaagaggct ggatcaaaac tgactctagc ggaaaatata
     4741 caattaaaac atcaaggcca gcaagttacc caaatgaaga tatacctcag catattcatt
     4801 tatcaattaa agagcctaat ataacaaatg aatattttgc agatttatat tttgatgatg
     4861 ataaattata cctagcccat aaaaaaaaat atggcaagat tgatagagct ggctctgaga
     4921 tattgagagt tttaattgac aatgaagtac aaattgcaga acatgatatt attttaggtt
     4981 taaacattcc tcattatcca tcactaaaac aatcaaataa cctttcaggt ttaaacattg
     5041 gcgaggatca gccttctttt acaccctatc atgcttttgg tgcagacaaa ggaacgcaaa
     5101 cctgcccagt ttgcaagtat ggtcgttatc atggaattat ttattttgtt ggtaaccacc
     5161 caaattggaa tgagataaaa acatggttaa tttttttaga taatgaaagt agattaagag
     5221 aaaaatattt gaaaacatat tttgtattag gcaatgaaaa agaatttata catgaaaata
     5281 cttttacctt tttagaaagc cttggcaaag agttgaattt gcaaaatatt gcattaactt
     5341 ttgtgccctc ctttaacgac tctagtactg aagtaaattt gaataaaata gatgccgaag
     5401 caaagaatac tattatcatt tataaacata gaactattgt agataagtat attaacctac
     5461 ttcccacaaa agaaaatttt gcaaaaatta ttaatgtact ggataaaaca aaaggcaatt
     5521 actttaattt agaagaacca aaacatgatt aaaatataat accatgtctg aattaaaaac
     5581 aaaaaagaac aacgcttcgg ttatagattt tattaatttg gttgaaaaag agcagaaaag
     5641 aaaagaatgt tttcagttat tagaactatt tgaaaccgtt accaaagaaa aagcaagtat
     5701 gtggggtagc agtattattg gttttggaga atataagtat aaatatgaaa gtggtagaga
     5761 aggggattgg ttttttgcag gcttctcacc cagaaaacaa aatattgtaa tttatataat
     5821 tgcaggtttt actgattatg aaagtttaat gcaacaattg ggtaaatata aaacaggctc
     5881 ctcttgttta tatattaaca aactggctga tattgattta aaagtgttga agcttttaat
     5941 agaaaaatct tttgcattta tgaaaaataa atatgcaaaa aattaaacta aacaataagt
     6001 cttcaaaatt ttatcttatt gttttttgca acacttttag gctaccatct attggttgta
     6061 atatttctaa atctaaaata gatgcaacta atggatagat atgaatattt tggaaagttt
     6121 ttattttttt acctttttta aaattttcac cccaagcaat aaaacttgct ttcatttcgg
     6181 gaatttcata actattaaat ccatgtttgc cataagttgg ctttttattt ttatttacta
     6241 ataatttaga accaacagga actaataata tatcccctat tctaccaatt gtatcttctt
     6301 tactactgta atttagtttt tcaggaaaat tatttgctaa ataaacattg taatcatctg
     6361 acttttcatt taataatttt tggtataatg gcagtacatc taatttatct tttgctgtaa
     6421 ccctcactaa ggtatagcta ttgattattg taaatttttt actatctatt tcaggtagtg
     6481 gaatataatt cttctcttct acagcaatca ttccatgatc cgaaacaaaa ataaaattaa
     6541 taggcaaatt aagtggttgt aaactatcaa ctaatttctt tatagcaaca tctatataat
     6601 gaacagaagc ttctgtctcc tttgcatctg ggccaaattt atgtccataa taatccactt
     6661 caggaaaata taaagtaatg aaatgtggcc gaatactatc tggcaatgtt aaccagtttt
     6721 taatgatatt tacttttgca tcccctttaa atttttcgtg ataatgatac caataggttg
     6781 gccttgtatt agcagcatta ctctctgagc caacccaaaa caaactagct gaaagcatac
     6841 cttgtttttc tgccaatgcc catagtggca agccattata ccaagtgcca tcaattactg
     6901 ttttagaacc aattttataa gtttcttttc tgatagaatc gtaaaaaata ttatctacga
     6961 taccatgatg tgaaggatac aaccctgtaa taatgctata atgatttggg aaagtaatgg
     7021 atggataaga aggatgcatg cctttttttg cccaaacacc tttagctgca atgtttaata
     7081 aatttttggc tttatatttt ttaaaataat cgtatctaaa tccatcggca gaaattaaaa
     7141 ttacataagg cttttgtagc gtttgttcag tattaactct gtttggtgtt accacctgat
     7201 tggtgtcaac cactttattt tgagcatata aaaaatttgc tgtaaaaagt acaaataata
     7261 aattcaaaaa tttcatttgg taataattat tagtagtcta ttg
//
LOCUS       DAOQHP010000069         7179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5492_length_7179_cov_4.371280, whole genome shotgun sequence.
ACCESSION   DAOQHP010000069 DAOQHP010000000
VERSION     DAOQHP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_7179_cov_4.371280"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1131
                     /locus_tag="PLY81_03500"
     CDS             85..1131
                     /locus_tag="PLY81_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNF29323.1"
                     /translation="MKNHFKILTPSLLILSILFTACERDQMPPLIPPSTGGDTTLNIL
                     AGSESGAYAANSAFIDFSTDIISTSGRASWDVAFYCGSDFKVGLNYKTVALTKQINKN
                     DLTNVTAADTIGMALSLSYSSTDFELLDSFSGNLEYTKIPVNTTAADNKVFIVQRGTG
                     AGIAAKPWYKIRVLKNSNISYTLQYARITETTFKTIEITKDNNYNFKFVSFDNGLVLS
                     EPKKDDWDIQYTAAVYKFPMGPVEIPYIFSDMVLINYLGGVQAAQVLNTQFSYNTITL
                     ANAQSLTYSNDRWAIADKWRTSTSSPTAGVKTDRFYVIKDQIDNYYKLKFISFHNTEG
                     GTRGKPKIAYELIK"
     gene            complement(1224..2426)
                     /locus_tag="PLY81_03505"
     CDS             complement(1224..2426)
                     /locus_tag="PLY81_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HNF29324.1"
                     /translation="MKTIKQLLLGMAVICTTVVTAQDKKVYKKVGCATVEPIQQVFAF
                     DTTTAGKRGLADNYYLWDNGKTLYVKIMNGSKKYKDMIKKYAKDWEKYANIKFEFVEV
                     GNTHIRVVLTGNDGGGNYSKLGTMALMVPQDEHTMHLDTTSFKTPEYTYTCIVHEFGH
                     AIGLMHEHMNPTSGIKWDKNRIYASYKLNQGWDKAMVDAQLFKKYSINYTNGTKYDPK
                     SIMHYPISRWETTDGFHVEWNNSISEGDKQLISALYPKKGARVNEVPRFTVSDFTQMQ
                     VERSDSKEGVSFFPSFTINTAGKSGRVYYLAVFTDDQGNMFETSSNEYKVFGVSGTSK
                     TSVLSPGQKIVVNKGKKDLELFIPYSEIPEKIRNKNIRASFYVFLYDNDEMKLLYASK
                     SVPCNMAR"
     gene            complement(2476..3681)
                     /locus_tag="PLY81_03510"
     CDS             complement(2476..3681)
                     /locus_tag="PLY81_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HNF29325.1"
                     /translation="MKKIKLIVLIALSSVFCVNLNAQKKIKQPVVQKNGCSSVQPIAN
                     VFLADTIPGRGLADNYYLWDNGKTIYVKFLSGSKLMQDKVKTIAKEWEKYANIKFKFV
                     EVGNSHIRINLDDKGGHNSTIGTLALSVPNDERTMNFDTTDFKTYAAMYRTVLHEFGH
                     ALGLLHEHYSPVSGIKWNKEAVYNELKKTQGWSKEMVDANLFQQFNQNYTNGTSYDSK
                     SIMHYPIKANWTTNAYSVNWNNKLSDGDIAMIKTLYPVGERENEVPRFVISNFTSLKV
                     QNNDNKQGVSIYPSFTLSTAGKSATVYFVAFFYDEDGNAIQKTTKSYSISGVTATFKS
                     FIIAADKTLNPNKNKASELELFIPYNELPVIKGKNNFQVEFKVYMLDGRELKLLYQSK
                     RKVFNFMKS"
     gene            3890..3963
                     /locus_tag="PLY81_03515"
     tRNA            3890..3963
                     /locus_tag="PLY81_03515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3924..3926,aa:Pro,seq:tgg)
     gene            complement(4084..5115)
                     /locus_tag="PLY81_03520"
     CDS             complement(4084..5115)
                     /locus_tag="PLY81_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNF29326.1"
                     /translation="MSTTKAPTLTAKDFATDQEVRWCPGCGDYSILAQVQKVMPGIGV
                     PKENIVFISGIGCSSRFPYYMNTYGMHSIHGRAAAIASGLKASRPELSVWIISGDGDS
                     MSIGGNHTIHFLRRNFDVNLLLFNNQIYGLTKGQYSPTSEANKITKSTPMGSIDHPFN
                     PLALAMGADATFVARSMDRDPKHLQYVLTRSHQHKGASFVEIYQNCNIFNDGAFETFT
                     EKATKADNTLFLEQNKPLVFGATQNKGIKLDGFKPIIVDLEEGASTNDLWIHDEHDFY
                     KAQILTRMFDDPSIEGHLPRPFGIFYQTQRACYEDVMAMQIEEAIAKKGVGDLNKLLK
                     GREVWEIAS"
     gene            5268..6662
                     /locus_tag="PLY81_03525"
     CDS             5268..6662
                     /locus_tag="PLY81_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF29327.1"
                     /translation="MATKVISVNIHKTIAFIKESFNSEDKDFTTGSIRKAVILLAIPM
                     ILEMSMESIFAVVDIFFVAKLGSYATATVGLTESMLAIIYSIGFGISMATTAMVARRI
                     GEKNYVEAANAAAQSILIAISLSIVITLFGIIYAKELLYLMGATKEIAEQGFLYTAIM
                     LGGNLVIILLFLLNGIFRGAGNAAIAMKSLWLANICNIILCPLFIHYYGLTGAAIATT
                     IGRSIGVIYQLHQLLKGKGFIRLYSKYFKPQLQIIKNLISVTTTATLQFIITSASWIA
                     MARIMTSFGNEAIAGYTIAIRLIVFFILPAFGLSNAAATLVGQNLGAKQPDRAAKSVW
                     KVAKYNAIYMGFVSVLFLFGAETFVKFFAHEIKVIETAVHALRVISLGYIFYGIGMVM
                     MNAFNGAGDTKTPTIVNFFWFWIIQIPMAYIAAKHFNWGEWGVYFAIVITETMVTITS
                     MYLFTKGKWKLVKI"
     gene            complement(6769..>7179)
                     /locus_tag="PLY81_03530"
     CDS             complement(6769..>7179)
                     /locus_tag="PLY81_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29328.1"
                     /translation="GFASGYSFMDDSKPNVNSSIYYRLKMVDKDGSFMYSKILIIKPA
                     GIKTVENLRIYPSIISSGNNVIWAEFISTKEQPITIQYYTVEGKIVTQLTQNAFKGNN
                     KIIITPNVVLNNGVNYIKVVTEGFEKVIPIVKYN"
BASE COUNT         2328 a         1123 c         1053 g         2675 t
ORIGIN      
        1 atgcctatgg tcgttcttat tttataacaa taaattttca gtggagtaaa aaataaatta
       61 ttaaatacat agaaatataa aaacatgaag aatcatttta aaatattaac cccatcctta
      121 ttgattttat ctattttatt tactgcttgc gaaagagatc aaatgcctcc tttaattcca
      181 ccttctacag gaggagatac tacattaaat attttagcag gaagtgaaag tggagcttat
      241 gcagccaact ctgctttcat agattttagt acagatatta ttagtacatc tggcagagca
      301 agttgggatg tagcctttta ctgtggcagt gattttaaag ttggattaaa ctataaaaca
      361 gtagctctta caaaacaaat taataaaaac gatttaacaa atgtaactgc tgccgatact
      421 attggtatgg ctttatcctt atcttattct tcaaccgatt ttgaactact agatagtttt
      481 tcaggaaatt tagaatatac aaaaattccg gtaaatacaa ctgctgccga taataaagta
      541 tttattgtac aaaggggaac aggtgcaggt attgcagcaa aaccatggta taaaataaga
      601 gtattaaaaa atagcaatat tagctacacc ttacaatatg caagaattac agaaacaact
      661 tttaaaacaa ttgagataac caaagacaat aattacaatt ttaaatttgt ttcttttgat
      721 aatggtttgg ttttatctga acctaaaaaa gatgattggg atatacaata tactgctgca
      781 gtttacaaat ttccaatggg cccggtagaa attccttata ttttttcaga tatggtttta
      841 attaattatt taggtggtgt acaagctgct caagtattaa acacacaatt ttcttataac
      901 accattactt tggctaatgc acaaagttta acttatagta atgataggtg ggcaatagca
      961 gataaatggc gtacatcaac atcaagccct acagcaggtg ttaaaacaga tagattttat
     1021 gtaataaaag atcaaattga taattattat aaactaaaat tcatcagctt tcataataca
     1081 gagggtggca caagaggtaa accaaaaatt gcttacgaac taattaaata attttctaaa
     1141 attcattgta ataattctaa gtggttaaat ataaaaagag gctacttatg ggtagcctct
     1201 ttaattttta ttaattttac ttattaccta gccatattac atggtacact tttagaggca
     1261 tataataatt tcatttcatc attatcgtat aagaaaacat aaaacgaagc tcttatattt
     1321 ttatttctta ttttttctgg aatttcagaa taaggaataa ataattctaa atcttttttg
     1381 cctttattca ctacaatttt ttgaccagga ctaagaacag atgttttaga tgtaccactt
     1441 acaccaaata ctttgtactc attactagat gtttcaaaca tattgccttg gtcatctgta
     1501 aacactgcta aataatatac tctaccactt tttcctgctg tatttattgt aaaggatggg
     1561 aaaaagctta ccccttcttt agaatcactt ctttcaacct gcatttgagt aaaatcgcta
     1621 acagtaaagc gtggcacttc attaaccctt gctccttttt taggatataa agccgatatt
     1681 aattgtttat ctccttcaga tatgctgttg ttccattcta catgaaatcc atcggtagtt
     1741 tcccaacgtg aaataggata gtgcatgata gatttaggat cgtattttgt gccattagta
     1801 taattaatgc tatatttttt aaataattga gcatctacca ttgctttatc ccaaccctga
     1861 tttaatttgt aagatgcgta aattctattt ttatcccatt taatgccaga cgttgggttc
     1921 atgtgttcat gcattaaacc aattgcatgc ccaaactcat gtacaataca agtataagta
     1981 tattcaggtg ttttaaatga tgtagtatct aagtgcatag tatgttcatc ttgaggtacc
     2041 attaaggcca ttgttcctaa tttagaataa tttccacctc catcatttcc tgtaagtaca
     2101 acacgaatat gggtgttacc aacttcaaca aattcaaatt ttatattggc atatttttcc
     2161 caatctttag catacttctt tatcatgtct ttatactttt tacttccatt cattatttta
     2221 acataaagtg ttttgccatt atcccacaga taataattat ctgctaaacc tcttttgcct
     2281 gctgttgtag tatcaaaagc aaatacttgt tgaatgggtt ctactgtagc acaaccaact
     2341 tttttatata cttttttatc ttgtgctgtt acaacggttg tgcatattac tgccatacct
     2401 aataatagtt gttttatcgt tttcattgtt ttcattgttt ataattttaa tgtttttata
     2461 tagatgtatt ttgttttaag atttcataaa attgaatact tttcttttag actggtataa
     2521 aagttttaat tctctaccat ctaacatata aactttaaac tctacctgaa aattattttt
     2581 tccttttatc acaggtaatt cgttataagg tataaaaagt tccaattcgc ttgctttatt
     2641 tttatttgga tttaaagttt tatctgcagc aataataaat gatttaaaag ttgcagttac
     2701 accactaata ctataagatt tagttgtttt ttgaattgca tttccatctt catcataaaa
     2761 aaaggcaaca aaataaactg tagctgactt gcctgctgta cttagtgtaa aagaaggata
     2821 tattgataca ccttgcttat tatcattgtt ttgaactttt aaagatgtaa agttagatat
     2881 tacaaaacga ggtacttcat tttctctttc tcctactgga tataaagttt taatcattgc
     2941 aatatctcca tcagataact tattgttcca atttacagaa taagcattag ttgtccaatt
     3001 tgcttttata gggtagtgca taatagattt agagtcgtaa cttgtaccat tggtatagtt
     3061 ttgattaaat tgttgaaata aattagcatc aaccatttct ttagaccagc cttgtgtttt
     3121 tttcagttca ttatatactg cttctttatt ccattttatt cctgaaactg gactataatg
     3181 ttcgtgtaac aaacctaatg catggccaaa ctcatgtaaa actgttctat acatggctgc
     3241 ataggtttta aaatcggtag tatcaaaatt cattgttctt tcatcatttg gtacagagag
     3301 agccaatgta ccaattgtgg aattatgccc acctttatca tctaaattta ttctaatatg
     3361 gctgtttcca acttctacaa acttaaattt tatattagca tatttttccc attctttagc
     3421 tattgtttta actttatctt gcattaattt actgccgctt aaaaacttta cataaattgt
     3481 ttttccgtta tcccacaaat aataattatc cgctagccct ctaccaggta ttgtatctgc
     3541 taaaaataca ttagcaatag gctgtacact gctacaacca tttttttgga ctactggttg
     3601 ttttattttt ttttgtgcat ttaagtttac acaaaacaca cttgataagg ctattaatac
     3661 tattaatttt atttttttca tattcgttta aattctattc tttaaaaaat taaacccttt
     3721 caataatagt aaaaagataa atatttttaa agttttacat attgatgcct gtttttatta
     3781 ctaaaatacc caataaccct tataaataag taagatattt taataaatat aaatacagaa
     3841 ttttttttta ttagtctgcc acctatattt gcacccctat ctcaaaattc gggaggtagt
     3901 tcagcccggt agaatacctg gtttgggacc agggggtcgc aggttcgaat cctgtcctcc
     3961 cgactttcaa aaagctcaca gtatttgtga gcttttactt tttgttgcat atttacacaa
     4021 aatctattcc aaataattat tcaacttatt attgtttaat aaaacaagcg ttattatttt
     4081 ggtttaggag gctatttccc aaacctctct accttttaac aatttattta aatctccaac
     4141 accctttttt gcaatagctt cttctatttg cattgccatt acatcttcat aacaggctct
     4201 ttgtgtttgg taaaaaattc caaaagggcg tggtaaatgc ccttcaatgc ttgggtcatc
     4261 aaacattcgg gttagtattt gtgctttata aaaatcatgt tcatcatgaa tccataaatc
     4321 attagtgctt gctccttctt ctaaatctac aataatgggt ttaaagccat ctaactttat
     4381 tcctttattt tgagtagcac caaaaactaa aggtttgttc tgctctaaaa acaaagtatt
     4441 atctgctttt gttgcttttt ctgtaaaagt ttcaaaagca ccatcgttaa aaatattaca
     4501 gttctgataa atttctacaa aagaggctcc tttgtgctga tggcttcttg ttaaaacata
     4561 ttgtaaatgt tttggatccc tatccatact tcttgcaaca aatgtagcat cggctcccat
     4621 agctaaagct aaaggattaa atggatgatc tatgctaccc attggagtgc ttttggttat
     4681 tttatttgct tcagatgttg gggaatattg tcctttggtt aggccataaa tttggttgtt
     4741 aaataataat aagtttacat caaaatttct tcttaaaaaa tgaattgtat ggttgcctcc
     4801 aatgctcata ctatcaccat caccactaat tatccaaaca cttaattcag ggcggctagc
     4861 ttttaaacca cttgctatag ctgcagctct accatgtata gagtgcattc cataggtatt
     4921 catataatat ggaaatctag aggagcaacc tattccacta ataaaaacaa tattttcttt
     4981 aggtacacca attcctggca ttactttttg aacttgtgct aaaatggaat aatcgccaca
     5041 gcctgggcac caacgtacct cttggtctgt tgcaaaatct ttagctgtaa gtgtaggtgc
     5101 ttttgtggta ctcattgcaa atttatttta tacacaaagg taaaggttag tgaaattata
     5161 aaagatacat aagtttttga ataaaggtat tattgcttaa ataaatttac tgatgaatct
     5221 ttacatttaa ttcattttga atttatattt atcaactaat ttttttaatg gcaaccaaag
     5281 ttatttcagt aaatattcat aaaactattg cttttattaa ggagtctttt aatagtgagg
     5341 ataaagattt tacaacagga agtattcgta aggcagttat tttattagct atacccatga
     5401 ttttagaaat gagtatggaa agcatatttg ctgtggttga catttttttt gttgccaaat
     5461 taggctctta tgcaacagca acagttggtt taacagaaag tatgctggca attatatact
     5521 ctattggttt tggtataagt atggctacaa ctgcaatggt tgccagaaga attggagaaa
     5581 aaaattatgt agaggccgca aatgctgcag cccaatctat tttaattgct atttcattaa
     5641 gtatagttat aacattattc ggaattattt atgctaaaga acttttatac ttaatgggtg
     5701 ctacaaaaga aatagcagag caaggatttt tgtacactgc aataatgtta ggtggcaacc
     5761 ttgtaattat attattgttt ttgttaaatg gtatttttag aggtgcaggt aatgcagcta
     5821 ttgcaatgaa aagtttatgg cttgcaaata tttgcaatat cattttatgc cctttgttta
     5881 tacattatta tggattaact ggtgctgcta ttgcaacaac aattggaaga agtataggag
     5941 ttatttatca acttcatcaa ctattaaaag gaaaaggttt tatacgttta tacagtaaat
     6001 attttaaacc acaactgcaa ataataaaaa atcttataag tgttacaact actgcaacac
     6061 tgcaatttat tattaccagt gccagttgga ttgctatggc tagaataatg acaagttttg
     6121 gaaacgaagc tattgcaggt tacacaatag caataagatt aattgtattt tttattctgc
     6181 cagcttttgg attaagtaat gctgctgcaa cacttgttgg tcaaaattta ggtgcaaaac
     6241 aacccgaccg tgctgcaaaa agtgtttgga aggtagccaa atacaatgcc atttatatgg
     6301 gttttgtatc ggtgcttttt ttatttggag cagaaacttt tgtgaaattt tttgcacatg
     6361 aaataaaagt aatagaaacc gctgtacatg ctttaagagt aataagttta ggatatattt
     6421 tttatggaat tggaatggta atgatgaatg cttttaatgg tgcaggcgat accaaaacac
     6481 caactattgt taattttttc tggttttgga ttattcaaat tccaatggca tatatagctg
     6541 caaagcattt taattggggc gaatggggtg tttattttgc aattgttatt acagaaacta
     6601 tggttacaat tacctccatg tatttattta ctaagggcaa atggaaatta gtgaagattt
     6661 agataatttt ttttgaattt catttcctaa taaaaatgtc attgctacaa tgtagcaatg
     6721 acattttaag tttataatgc aacttaatta aaacttacct tctgttattt agttatattt
     6781 tacaattggt attactttct caaacccttc tgttacaacc ttaatataat ttacaccatt
     6841 attcaaaact acattaggtg ttattattat tttattgttg cccttaaaag cattttgtgt
     6901 aagttgtgta actatttttc cttcaactgt ataatactgt atagtaatag gctgctcttt
     6961 ggtgctaata aactctgccc aaattacatt gttaccagat gaaataattg aagggtaaat
     7021 tcttaaattt tctactgttt ttatacctgc aggttttatt atcaaaattt ttgaatacat
     7081 aaaactgcca tctttatcta ccatttttaa acgataataa atagatgaat taacatttgg
     7141 tttactatca tccataaaag agtagccgga agcaaatcc
//
LOCUS       DAOQHP010000070         7147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5519_length_7147_cov_3.357163, whole genome shotgun sequence.
ACCESSION   DAOQHP010000070 DAOQHP010000000
VERSION     DAOQHP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_7147_cov_3.357163"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLY81_03535"
     CDS             complement(<1..612)
                     /locus_tag="PLY81_03535"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF29329.1"
                     /translation="MRKMLLLLTCLIVLYSTSIAQYCNSSFSNASFEHITNVTFGGVN
                     NSSTGNTGGPVNYLNKVAYVYYNNSHNLSVSILADASEYVYAFIDWNQDGDFADSDET
                     YTVASNVSTTGPFVLSIATPLTALAGNTRMRVMVDYNGSTPDPCKNSTYGEAEDYTVN
                     VGNMPACMPPTNLAANPVGISTASVSWDASVSNPTGGYQFELRT"
     gene            complement(736..1311)
                     /gene="lptC"
                     /locus_tag="PLY81_03540"
     CDS             complement(736..1311)
                     /gene="lptC"
                     /locus_tag="PLY81_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNF29330.1"
                     /translation="MISNFKHITFQKAVLVVGCFFVFSCENKIETVRDLGKATLGVEE
                     GKNIESFISQSGKMRAKLNAPIMLRYLLDTPKIEFTKTLFVSFYDDTLGIESNLFAKY
                     GRYLENDNKVYLKDSVVVYNIAGDTLWTSELYWNQDTEEFFTNKPVRVKREFNNKYIH
                     SIGVRANQNLKDITFYKIQPDSYFIVSDSIY"
     gene            complement(1304..2671)
                     /locus_tag="PLY81_03545"
     CDS             complement(1304..2671)
                     /locus_tag="PLY81_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29331.1"
                     /translation="MQLAKTIINRFTATVIFTVCFIFITAAQENSPFSRYGLGDLTNT
                     FQTLNNGMGGVAVAYNDLQSINFNNPASFASTRIVTYDLGVTIDSRILRSKAPVNKYN
                     SANFSPAYIAVATPISSKHSFGLAFGFKPVTRISYNIIRNERLAGIDSVQSLFEGTGG
                     MNEVFVGVGKKWKNLAIGLSTGYHFGKKQNNTKRGFINDTVVYYKANYNTSTTFGGVF
                     LMGGFQYDILLSDSAKNRKITKPIKDFYYLKLGGTFSLGHQLNAEQTSFKETFDYDYS
                     GGSYTIDSLNVGNTIKGKIKIPANFGFGFLFQKTSVDISGVYDRWAIGADLLLSNWKN
                     YRFYDKKDATVNSWQFKIGGQWTPDAKNTKSYFSRVAYRAGLNVGKDYINADGKEMKT
                     FSFSIGLGLPVRSTRFSYQYSTIHTALEIGKRGSAANNITENFFKLSVGFSLSDIWFI
                     KRKYD"
     gene            complement(2646..3371)
                     /locus_tag="PLY81_03550"
     CDS             complement(2646..3371)
                     /locus_tag="PLY81_03550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNF29332.1"
                     /translation="MQTTLCFDFGNTRLKCAVFKGAELLQVEVLENDKVANVEVLLKK
                     YQPQKSIFSSVINHNKAIEELLQQKTTFHQLNANSKLPFTTPVGKPETIGADRLAICA
                     GAIHLYPNNHNLAIGLGTCITFNFINKFHEFLGGSISPGMNMRFKAMHQQTALLPLVE
                     ATHNFPLIGYDTKTNLLSGVLLGMAKEIDGIIEAYSKKYNKFNVLITGGDMAFFVSHL
                     KNKIFADPYLIFKGLYAISENNN"
     gene            complement(3408..4868)
                     /locus_tag="PLY81_03555"
     CDS             complement(3408..4868)
                     /locus_tag="PLY81_03555"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF29333.1"
                     /translation="MSNKVAIIGAGPAGLSAAYLLGKAGISTTIFEQDKQYVGGISRT
                     ENYKGYYFDIGGHRFFSKSKEVEDFWTEILQDDLLERPRSSRIYYNHTFFAYPLVALE
                     ALKKLGLWQSFLCVASYLKAKLFPVKNPQNVEDWVSNQFGKKLYSIFFKTYTEKVWGI
                     SCKDISADWAAQRIKGLSLSSAIKNALIKPSYANKEKVIKTLIDSFRYPKKGPGMMWE
                     TCRDKCLQLGVKVKMNAEVIGLNFNDNLWQVVMKNEQFDEKFTYVLSSASIKNIVPNI
                     LPSVNKVAKEAALQLRYRDFLTVVLICKDKDSFTDNWIYIHDPSVKVGRIQNFKSWSP
                     YMVPDESMACYGLEYFCFEGDGMWQKNNDDLIDLATNEMHQIGLLNKENVVEGYVVRQ
                     PKAYPVYDKNYKEKVQLVRDGLKPYKGLYLIGRNGMHKYNNQDHSMMTAMFSAKNIIA
                     GKELYDVWKVNQDAEYQEEGERGVNEGGRLIPIVQN"
     gene            5021..6202
                     /locus_tag="PLY81_03560"
     CDS             5021..6202
                     /locus_tag="PLY81_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29334.1"
                     /translation="MRLNFTIIFNLIFFFSLNSFSNISFAQYSTTNNKNKNNSHTKNI
                     TGVWRGYFIQREFDLLTKKFHEERYKYEVQINNTQTSKLQGVTYSYLDKRFYGKTALQ
                     GIFTVKSNNVLIKESKMLELKMTGNSEPCLMTCYLTYSKIGDKEILKGDYSSTINSSK
                     SDCGEGIVYLEKVPDSDFGKEDFLVNKPNSSLSKSSKNSSPNKKINSAKLKYKPGAED
                     ALVTNNKPIIKKEATPKLVDKIEKKESNQPTAPLEKIEKIEKPITPLPKVLTERKNNL
                     VKTFYVGEGEISLELYDNGQIDNDIVSIYHNGVPVIQNERLSTIPIKLNIKITASEPV
                     QEFIIVAESLGEIPPNSSLLKIKAGNKTYQVFLTSDLQKNAKIVFEYKPQNEDSQTPK
                     K"
     gene            complement(6212..>7147)
                     /locus_tag="PLY81_03565"
     CDS             complement(6212..>7147)
                     /locus_tag="PLY81_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29335.1"
                     /translation="VYYATAMILLAIIIYAIGVYKKINFSTQIPKQNIFYLIFSLLFA
                     VLFNVLYYYWLFSFSVPTENNLPVNPFAIKNFTQAFAAIFHLTHFNFHFYLIVVVAFY
                     LMFKKYKISSTLNWLNTAPIAIFILFFLIYFIHGSVIIQRIYLVLIPVFSLFVAICFN
                     ELCTKKWISKRLNLFLLLNCITLLLSFYVAKQKVVKNNIESIHTQDLINHYYLFNYNP
                     YQASLVAQKIAKATKTNLFFQEGFGDSGIKYYLKESNINYSELQESTLLIENKFTLLT
                     NNKKWIEELLVKHHWNLQKKLSNLNYYTVYLCSKN"
BASE COUNT         2557 a         1181 c          949 g         2460 t
ORIGIN      
        1 tgttcttaac tcaaattgat agccaccagt agggtttgat acagaagcat cccaagaaac
       61 cgatgctgta cttatgccaa ctggatttgc agctaaattt gtagggggca tacaagcagg
      121 catgttgcct acatttactg tataatcttc tgcttcgcca taagtagaat ttttacatgg
      181 gtctggagtg gatccgttat agtcaaccat cactctcatt cttgtatttc ctgcaagtgc
      241 agttaaagga gtagctatac ttagaacaaa aggtccagta gttgaaacat tggaagcaac
      301 tgtataagtt tcatctgaat ctgcaaaatc accatcttgg ttccaatcta taaatgcata
      361 cacatattca cttgcatcag ctaaaatgga aaccgacaaa ttatgagaat tgttgtaata
      421 aacataagca accttattta aataatttac agggcctcct gtattaccag tagaagaatt
      481 attgacccca ccaaaagtta cattagtaat gtgctcaaaa gatgcattac taaaagaaga
      541 gttgcaatac tgggctatac ttgttgaata caatacaatt aaacaagtaa gcaagagtaa
      601 catttttctc ataataagtt aattttaagg cttcaatgat ataacttttt ttttttaaaa
      661 aaaataaaaa gaggataacc tcctattaat tttatacaag cgttaaaaat gtaaagaaaa
      721 cagatgggaa attactcaat agatactatc tgaaacaata aaataactat caggctgaat
      781 tttataaaat gtgatgtctt ttaaattttg gttagctctt acacctatac tatgaatata
      841 cttattatta aactctcgtt tcactcttac tggtttatta gtgaaaaact cttctgtatc
      901 ctgattccag tacaactcag aagtccataa agtatcgcca gcaatattgt aaaccactac
      961 actatctttt aaataaactt tattatcatt ttctaaatac ctgccatatt tagcaaacaa
     1021 attgctttca ataccaagcg tatcatcata aaaacttaca aacaaagttt tagtaaattc
     1081 tattttaggg gtatctaata aataccttaa cataattgga gcatttagtt ttgctctcat
     1141 tttaccagat tggctaataa agctttctat attcttccct tcttctactc ctaaagttgc
     1201 ttttcctaaa tctcttacag tttctatttt attttcgcaa gaaaacacaa aaaagcagcc
     1261 taccactaag actgcctttt ggaaagtaat atgtttaaaa ttgctaatca tatttacgtt
     1321 taataaacca aatatcactt aagctaaagc caacagaaag tttaaagaaa ttttcggtta
     1381 tgttgtttgc tgcactacct cttttaccta tttctaatgc tgtatgaatt gtggaatatt
     1441 ggtagctaaa ccttgtagaa cgtacaggca aacctagtcc aatagaaaaa gaaaaagttt
     1501 tcatttcttt tccgtcagca ttaatataat ctttacctac attcaatcct gctctataag
     1561 ctactctact gaaatagctt ttggtatttt tagcatctgg tgtccattgc cctcctattt
     1621 taaattgcca actatttaca gtagcatctt ttttatcgta aaaacgataa tttttccaat
     1681 tagataaaag taaatctgca ccaatagccc atctatcata aacgcctgaa atatctacag
     1741 aggttttttg aaataaaaat ccgaaaccaa aatttgctgg aattttaatt ttacctttaa
     1801 tagtattacc cacatttaaa ctatcaatag tgtaagagcc accagaatag tcataatcaa
     1861 atgtttcttt aaatgaagtt tgctctgcat ttagttgatg gcctagagaa aaagtaccac
     1921 ctaattttaa gtaataaaaa tctttaatag gctttgttat ttttctattc ttagcagaat
     1981 cggataaaag tatatcgtat tgaaatcctc ccattaaaaa gactccacca aatgttgtag
     2041 atgtattgta attggcttta taatatacta ccgtatcgtt aataaaacca cgtttggtat
     2101 tgttttgctt tttaccaaaa tgataaccag tagataaccc aattgctaag tttttccatt
     2161 tttttccaac cccaacaaat acttcattca tgccacctgt accttcaaat aaagattgaa
     2221 cactatcaat tcctgctaat ctttcatttc taataatatt atagcttata cgagttacag
     2281 gtttaaaacc aaaagctaaa ccaaaagagt gtttagaact aattggggtt gcaactgcaa
     2341 tatatgcagg tgaaaaattg gctgaattgt atttatttac tggagcttta cttcttagta
     2401 ttctggaatc tattgtaaca cctaaatcat aagtaacaat tctggtactg gcaaaagatg
     2461 caggattatt gaagttgata gattgaagat cattataagc tacagcaaca ccacccatac
     2521 cattatttaa ggtttgaaaa gtattcgtta aatcacccaa tccgtatctg gaaaatggtg
     2581 aattttcttg tgctgctgta atgaagatga aacaaactgt aaaaattaca gtagctgtaa
     2641 atctattaat tattgttttc gctaattgca taaagtcctt taaatattaa ataagggtcg
     2701 gcaaatatct tgtttttaag atgagagaca aaaaaagcca tatcgccacc tgttattagc
     2761 acgttaaatt tgttatattt tttagaataa gcttctataa taccatctat ttctttagcc
     2821 atacctaata aaacaccact taataaatta gttttagtat cgtagccaat taaaggaaaa
     2881 ttatgagtag cttctaccaa aggcaataaa gctgtttgtt ggtgcattgc tttaaaacgc
     2941 atgttcatgc ctggtgaaat gctaccacct aaaaactcat gaaatttatt gatgaaatta
     3001 aaagtaatac aagtacctaa gcctattgcc aaattgtggt tattagggta caaatgtata
     3061 gctcctgcac aaattgccaa tctatcggca ccaatagttt ctggcttgcc aactggtgtg
     3121 gtaaatggta atttgctgtt tgcatttaat tggtgaaaag tagttttttg ttgtaacaac
     3181 tcttctattg ctttattatg atttattact gaggaaaaaa ttgatttttg tggttgatat
     3241 ttttttaata gtacttcaac gtttgccacc ttatcatttt ctaatacctc tacttgcagc
     3301 agctctgccc ctttaaaaac agcacatttt aatcttgtat tcccaaaatc gaagcaaagt
     3361 gttgtttgca taatttttta taagttaaaa aatattttta atcaaagtta gttttgtaca
     3421 attggtatta gccgtcctcc ttcatttaca cctctttcgc cttcttcctg gtattctgca
     3481 tcttggttta ctttccaaac atcatataat tcttttcctg caataatatt tttagctgaa
     3541 aacatagcag tcatcatact atgatcttgg ttattatatt tatgcatacc atttctgcca
     3601 attaagtaaa gacctttata aggttttaat ccatctctaa ctaactgcac tttttcttta
     3661 taatttttat cgtacacagg ataagcttta ggctgcctaa ctacataccc ctctactaca
     3721 ttttctttgt ttaataatcc tatttggtgc atttcattgg ttgctaaatc aattaaatca
     3781 tcattgtttt tttgccacat gccatctcct tcaaaacaaa aatactctaa accataacat
     3841 gccatacttt catctggtac catatatggg ctccaacttt taaagttctg aattcttcca
     3901 actttaactg atgggtcgtg aatataaatc caattatcgg taaagctgtc tttatcttta
     3961 caaattaata ctactgttaa aaaatctcta taccttaatt gtaacgcagc ctcttttgct
     4021 actttgttta ctgaaggtag aatatttggc actatatttt ttatagatgc tgaggaaagg
     4081 acgtaggtaa acttttcgtc aaattgttca tttttcatga ctacttgcca aaggttatca
     4141 ttaaaattaa gacctataac ttctgcattc atttttactt taacacctaa ttgcaaacat
     4201 ttatctctac aggtttccca catcattcct gggccttttt taggatatct aaaagaatct
     4261 attaatgttt taattacctt ttctttatta gcgtaactag gttttattag tgcatttttt
     4321 attgcagagg ataatgataa acctttaatt ctttgagctg cccaatctgc cgaaatatct
     4381 ttacagctta taccccaaac tttttcggta tatgttttaa aaaaaataga atataatttt
     4441 ttaccaaact gattagaaac ccagtcttct acattttgag ggttttttac aggaaaaagt
     4501 tttgctttta aataacttgc aacacaaaga aaagattgcc aaagccctaa ttttttgagt
     4561 gcttctagag caactaatgg atacgcaaag aatgtatggt tgtaataaat tctactactt
     4621 cttggccttt ccagtaaatc atcctgtaaa atttctgtcc aaaaatcttc tacctctttg
     4681 ctcttgctaa aaaacctatg tccaccaata tcaaaataat atcctttata gttttcagtt
     4741 cttgaaatac cacccacata ttgcttatcc tgttcaaaaa tagttgtact aattcctgcc
     4801 ttgcctaata aataagcagc tgataaacct gctggacctg caccaataat tgccacctta
     4861 ttgctcattc aatttcaatc aaaatttata aagccgtgaa aatattactt agatgccaat
     4921 aaataaaaat aaaaactaca aaggttaaat aacttttaaa taaataacct ttaacgattc
     4981 tttgagtgta aagtattgca tataaattta gatttgcacc atgagattga attttacaat
     5041 aatcttcaac ttaattttct ttttttcact aaactctttt agtaatatat cttttgctca
     5101 atattctaca acaaacaata aaaataaaaa caactcacat actaaaaata ttacaggtgt
     5161 ttggcgtggg tactttattc aaagagaatt tgatttactt accaaaaaat ttcatgaaga
     5221 acgttataaa tacgaagttc aaataaacaa tactcaaacc agcaaactac aaggtgttac
     5281 ttactcttat ttagacaaaa gattttatgg taaaacagca ttacaaggaa tttttactgt
     5341 taaatctaac aatgttttaa taaaagaaag taaaatgtta gagctaaaaa tgactggcaa
     5401 ttcagagcca tgcttaatga cttgttattt gacttattct aaaattggag ataaagaaat
     5461 attaaaagga gattattcaa gcacaataaa ttcttcaaaa agtgattgcg gcgaaggtat
     5521 tgtatattta gaaaaagtac cagatagtga ttttggaaag gaagattttt tagtgaataa
     5581 acctaactca tctctaagca aatcctcaaa aaattcttcg cctaataaaa aaataaattc
     5641 agcaaaatta aaatataagc caggtgccga agatgcatta gttacaaaca ataaaccaat
     5701 aataaaaaaa gaagcaaccc caaaactggt tgataaaatt gaaaagaaag aaagcaatca
     5761 acctactgcc ccacttgaga aaattgaaaa aatagaaaaa cctataaccc cacttcctaa
     5821 ggttttaaca gaaagaaaaa ataatttagt aaaaaccttt tatgttggcg aaggtgaaat
     5881 aagtttagaa ttatacgata atggacaaat agataatgat attgtatcta tttaccacaa
     5941 tggtgtaccg gttatacaaa atgaaaggtt aagcactatt ccaattaaac taaatattaa
     6001 aattactgct tctgaacctg tacaagaatt tattatcgtt gctgaaagtt taggagaaat
     6061 tccgcctaat tcatctttat taaaaattaa agctggtaat aaaacttatc aagttttctt
     6121 aacctccgac ttgcagaaga atgctaaaat tgtttttgaa tataaaccac aaaacgaaga
     6181 cagccaaaca cctaaaaaat aattacccaa cttaattttt actgcataaa taaacagtat
     6241 aatagttaag gttgcttaat tttttttgaa gattccaatg gtgcttaacc aatagctctt
     6301 caatccattt cttgttattg gtaagcaatg taaatttatt ttctattaaa agagtacttt
     6361 cttgcaattc actataattt atattagatt cttttaaata atactttata ccgctatctc
     6421 caaatccttc ctgaaaaaat aaatttgttt ttgttgcttt agcaattttt tgtgcaacta
     6481 atgaggcttg ataaggatta taattaaata aataataatg gttaattaaa tcttgtgtgt
     6541 gtatagattc tatattattt ttaacaacct tttgtttggc tacataaaaa gaaagtagca
     6601 atgtaataca gttcaaaagc aaaaataaat ttaatctttt gcttatccat ttttttgtac
     6661 aaagctcatt aaagcaaatt gcaacaaaca aggaaaatac aggaattaaa accaaataaa
     6721 ttctttgaat aattacagaa ccatgtataa aataaattaa aaagaaaagt ataaaaatag
     6781 caattggtgc agtatttaac caatttaaag tgctactgat tttatacttt ttaaacatca
     6841 gataaaaagc caccacaaca atcaaataaa agtgaaaatt aaagtgtgtt aagtgaaaaa
     6901 tagctgcaaa tgcttgtgta aaatttttaa ttgcaaatgg gttaactggt aaattatttt
     6961 ctgttggaac agagaagcta aataaccaat aatagtaaag tacattaaat aatactgcaa
     7021 acaataaaga aaatattaaa taaaaaatat tttgcttagg tatttgtgta ctgaaattaa
     7081 tttttttata tacaccaata gcataaataa ttattgccaa caaaatcatt gctgtagcat
     7141 aatatac
//
LOCUS       DAOQHP010000071         7143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5523_length_7143_cov_3.555869, whole genome shotgun sequence.
ACCESSION   DAOQHP010000071 DAOQHP010000000
VERSION     DAOQHP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_7143_cov_3.555869"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1394)
                     /locus_tag="PLY81_03570"
     CDS             complement(192..1394)
                     /locus_tag="PLY81_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29336.1"
                     /translation="MKHFLFFILFSQLTMFGSAQSNNSDSSQTKTFKVAIFAPIFLDS
                     AFNGTFFKISGNNSLPKTILPGLDFYNGVMMAIDSLQREKMPLEILFFDTKSKDLPMK
                     KILTNNVWDSVSLIIASFKDRHEIKPMADFAKLKKIPIISATYPNDGGVNDNPYFVLL
                     NSTLRTHCEGVYKYIQKNFSTNNLIYIKRHGRVENEIEKIFREIGEITPAIPLKYKTV
                     ILTDSLNQQDLRKVLDSSKTNVIICGSVNENFGIRLVKTLSTLKGTKSIAIGMPTWDG
                     IKEFNNLSMDEATKGVEIVYSSPYYFPRKEAFGKYITEQYKDKFFARASDWFLKGYET
                     TYNFCHLLVKYQQDFMQQLTSTEYKVFTDYNIQPLLNKNEVPSIDYFENKNLYFYRKQ
                     DGILKSVH"
     gene            complement(1455..2990)
                     /gene="guaA"
                     /locus_tag="PLY81_03575"
     CDS             complement(1455..2990)
                     /gene="guaA"
                     /locus_tag="PLY81_03575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNF29337.1"
                     /translation="MTSKILILDFGSQFTQLIARCVREANIYCEIIPYNKKFTINNSL
                     KGIILSGSPFSVNDINNPEVDIQSFIEHVPILAVCYGAQLTAKMFGGRVDKSNKREYG
                     RAVLKIQQADTLFNSVSKNTQVWMSHSDSIVELPKGFEVLATTESIPVAAFKNTDLDV
                     NPLYGLQFHPEVYHSTDGKQMIQNFLINICKCSQDWTPASYVVETVEALKKQIGNAHV
                     IMALSGGVDSTVAAMLISKAIGNRLHGIFVDNGLLRKNEFEQVLESYKQLQLNVTGVN
                     AKQLFYDALKNKTEPEEKRKIIGKLFIDVFQEESKKVKEIKFLGQGTIYPDVIESVSV
                     HGPSATIKSHHNVGGLPDTMHLELVEPLRFLFKDEVRKVGLELGIPADLINRHPFPGP
                     GLAIRILGEITEDKVKLLQDADNIYIQNLKETNLYQKVWQAGTILLPVKSVGVMGDER
                     TYEFTVALRAVTSVDGMTADWAHLPYEFLADVSNEIINNVRGINRVVYDISSKPPATI
                     EWE"
     gene            complement(3069..4955)
                     /locus_tag="PLY81_03580"
     CDS             complement(3069..4955)
                     /locus_tag="PLY81_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF29338.1"
                     /translation="MKNFKNIYFVLLLLTFSLTSYSQMINGRVINERGKAAANISVKF
                     TNKANTISTNKDGSFKIMATKLPDTLIFSAEGYESYKVIVTEKTVKDPDFSIVLLTKR
                     EKSSDPTVEGAGFLEGAEMLTMAGRGLVSAGTVSSSDTYTKTSDASKHKEVSTVSYGK
                     IKSTRAMGSASDGGKSKTYYYEDSVEVAKDQYETKLLTAGEVNDFSKWKMWEDYTDKD
                     FATYSNYWKLFAKKRYCVQVQTNNYYAAIGQKVYLLDANTLDTVWQAITDNTGKSELW
                     GRMNTTTSQNNNYIIVCEGESVKIKTPYLFHEGINRITLTKSCVARNNVEIAFVVDAT
                     GSMGDEIEYLKLELEDVLTKTFNRYNNLELKASAVFYKDRGDDYVTKHINFNNDLLKM
                     LNFIKLQQAGGGGDMPEALDAALEVAIDSLSWSSDARAKIMFVVLDAPAHDGTQNKIY
                     SLIQKAAAKGIRIVPIVCSGADKNTEFLMRSVALATNGTYLFLTNHSGVGDKHIEPTT
                     DAYSVELLNSLLPRIIQQMIYANTCVEQEKSEPIVKTNNNILNIKVSPNPTKGQVTIT
                     TTKPLKEIYIADFTGKILMRIPVNIKQTSWSINIAHFPAGTYIVKYVTTENKWGAEKI
                     ILVH"
     gene            5080..6774
                     /locus_tag="PLY81_03585"
     CDS             5080..6774
                     /locus_tag="PLY81_03585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNF29339.1"
                     /translation="MKKVILFVFVLIISAKFSFSQKTVANGWHLLNYQQDSVYGIDLN
                     RAYAFLLEKNKKATPVIVAVIDSGIDTAHEDLKNILWKNTKEIPGNGIDDDKNGYIDD
                     IYGWNFLGNKDGRNIKKASSEKSRIFHTYKNKYENKELDTNLLSKDEKYIYKTWLRSA
                     AEINASTQEQANITYIELALKSLIKNDKIVREDMGKEEYTFEELEIYIPKTNDAKRAK
                     VGVLQIIKLFSLDSKSKNTEVIEEISEYVEGKKASFEEAEKAPFDYRADIVKDNTKDI
                     NDKNYGNNDVMASTPKHGTHVSGIIAAQRNNNLGMDGVADNVKIMTIRAVPDGDEYDK
                     DVALAIFYAVDNGAKVINMSFGKGFSPEKKWVDSAVQYAASKDVIILHAAGNDSKNID
                     SSENFPNPFMYYTGKRAPNYVTVGASSDPKISGSLAASFSNYGINTVDVFAPGDKIYS
                     TIPGGNKYGNLQGTSMATPVVAGIAALLRSYYPSLSAVQIKNIIEKSATIYDNSVATF
                     LPGTNDKTTLSELCRTGGLVNAYAAVQLAEKTVPEVNKVEENKLPIKPTKKVKKKN"
     gene            6774..>7143
                     /locus_tag="PLY81_03590"
     CDS             6774..>7143
                     /locus_tag="PLY81_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF29340.1"
                     /translation="MATPKEQDYPAYYAQYLAKINAANILDLMSKYAGLIEQFIHEIP
                     DDKANFAYAKNKWTIKEVLQHLIDSERVFVYRAMRFARKDFIELEGYDENEYTKNAHA
                     NCRDFSILKQEFLLLRKTTDL"
BASE COUNT         2411 a         1143 c         1029 g         2560 t
ORIGIN      
        1 ccttttgatt caatcttgtc aaaatatttg ctaacaagag caactctaaa tgggtcgcca
       61 actgtaatta cagtatctgc caattcatct ggtgtaatat ctaaatgata cacagcacct
      121 ctactattaa taattaattc tgaatctgct attctcttca tataaaattt gttttatttt
      181 ttagttatta tttagtgtac cgattttaaa attccatctt gttttctata aaagtataaa
      241 tttttatttt caaaataatc tatactcggt acttcatttt tgttgagtaa tggttgaata
      301 ttataatctg taaatacttt atattcggta ctggttagtt gctgcataaa atcttgctga
      361 tacttaacta gcaaatgaca aaagttgtat gttgtttcat aaccttttaa aaaccagtcg
      421 ctggctcttg caaaaaattt atctttatac tgttctgtta tatattttcc aaaagcttct
      481 tttcttggaa aataatatgg agaagaataa actatttcta caccttttgt tgcttcatcc
      541 attgataaat tattaaattc ctttattcca tcccatgttg gcatgccaat agcaattgat
      601 tttgtacctt ttaatgtact caatgttttt actaatctta taccaaagtt ttcatttaca
      661 ctaccacaaa taattacatt tgttttagaa ctatctaaaa cttttctcaa atcttgctgg
      721 tttagagaat cggttaaaat aactgtttta tattttaatg gaattgctgg tgttatttct
      781 cctatctctc taaatatttt ttcaatttca ttttcaacac gtccatgcct ttttatataa
      841 attaaattat tagttgagaa atttttttga atatatttat aaactccttc acaatgggta
      901 cgtaaagtag aatttaatag aacaaaatat ggattatcgt ttacaccacc atcgttagga
      961 taggtagcag atataattgg aatttttttt agcttggcaa aatctgccat aggttttatt
     1021 tcatgcctat ctttaaaaga tgcaataatt agagaaacac tatcccaaac attgttggtt
     1081 aaaatttttt tcataggtaa atctttactc tttgtatcaa agaataaaat ttctaatggc
     1141 attttttctc tctgtaaact gtcaatagcc atcattacac cattataaaa atctagccct
     1201 ggtaatattg ttttaggcaa gctattattt cctgaaattt taaaaaaggt gccattaaat
     1261 gcagagtcta aaaaaattgg agcaaagatt gctaccttaa aagtttttgt ttggctgcta
     1321 tccgagttat tggattgtgc actaccaaac atggttaatt gagaaaataa aataaaaaat
     1381 aaaaaatgct tcattagtaa aataataatt acttataaac aggtataaat atagtgccaa
     1441 aaaagataaa aagtttactc ccactcaatt gttgctggtg gcttactgct aatatcgtaa
     1501 acaactctgt taattcctct tacattatta ataatttcgt tagatacatc tgctaaaaac
     1561 tcgtagggca aatgtgccca atctgcagtc ataccatcta cacttgttac agctcttaaa
     1621 gcaacagtaa attcgtaggt tctttcatca cccattactc caacgctttt tactggcaac
     1681 aaaattgttc ctgcttgcca tactttttgg taaaggttag tttcttttaa attttgaata
     1741 tatatattgt cggcatcttg caaaagcttt actttatctt cagtgatttc accaagaatt
     1801 ctaatggcta accctggtcc tggaaatggg tgcctgttta tcaaatctgc aggaatgcct
     1861 aattctaagc ctacttttct tacttcatct ttaaataaaa aacgcaaggg ttctaccaac
     1921 tccaaatgca ttgtatctgg caagccacct acattatggt ggcttttaat tgttgcacta
     1981 ggaccatgaa cacttacact ttcaataaca tctggataga ttgtaccttg cccaagaaat
     2041 tttatttctt tcactttttt tgactcttcc tgaaaaacat caataaatag tttaccaata
     2101 atttttcttt tctcttcagg ctctgttttg ttttttaatg catcataaaa taattgctta
     2161 gcattaacac ctgttacgtt taattgtaat tgtttataag attctaaaac ctgttcaaat
     2221 tcattttttc ttagcagtcc attatctaca aaaatgccat gtaatctatt accaattgct
     2281 ttactaatta gcattgctgc aacagtgcta tcaacaccac cacttaatgc cataataaca
     2341 tgtgcattgc caatttgctt ttttaaggct tctacagttt ctactacata cgaagcaggt
     2401 gtccaatctt gtgaacattt gcaaatattg attaaaaagt tttgaatcat ttgctttcca
     2461 tcagttgaat gatatacttc tggatggaat tgtaagccat ataatggatt tacatctaaa
     2521 tcggtatttt taaaagcagc tacaggaata ctttctgttg ttgccaatac ttcaaaccct
     2581 ttgggaagtt ctacaatact atcgctatgg ctcatccata cttgagtatt ctttgaaaca
     2641 ctattaaata aggtatctgc ttgttgaatt tttaatactg cccttccgta ttctctttta
     2701 ttacttttat ctactctacc accaaacatt tttgcagtaa gttgtgcccc atagcataca
     2761 gctaaaatag gtacatgctc aataaaagat tgtatatcaa cttcaggatt atttatatca
     2821 tttacactaa atgggctgcc actaagtatg atacctttta aagaattgtt tatagtaaat
     2881 tttttattgt aaggaataat ttcacaataa atatttgctt ctctaacaca acgagcaata
     2941 agctgtgtaa attggcttcc aaaatctaaa attagaatct tacttgtcat gctgcgaaga
     3001 taagcctttg cagaaaaaag gcttatccta attagaataa gccttttgtt aaatacacat
     3061 ttttgaggtt aatgtactaa aataattttt tcagcccccc atttgttttc tgttgtaaca
     3121 tattttacaa tataagtacc agcaggaaaa tgagcaatat ttatagacca gcttgtttgt
     3181 tttatgttta caggaatacg cattaaaatt ttacctgtaa aatcggcaat ataaatttct
     3241 tttaaaggct tggtagtagt tatagtaacc tgccctttgg ttgggtttgg actaacttta
     3301 atatttaaaa tattattgtt agtttttaca attggttcag atttttcttg ctctacacaa
     3361 gtatttgcat aaatcatttg ttgaataatt ctaggcaata aactatttaa taattctaca
     3421 ctatatgcat ctgttgtagg ctcaatatgt ttatctccta ctccactatg atttgttaaa
     3481 aataaatagg taccattagt tgccaaagct acagaacgca ttaaaaattc agtattttta
     3541 tcagcaccac tacatacaat tggtacaata cgaattcctt tagcagcggc tttttgaatt
     3601 aaactgtata ttttattttg tgttccatca tgtgcaggtg catctaaaac tacaaacatt
     3661 attttagctc tagcatcact gctccaactc aagctatcta ttgctacttc taaagcagca
     3721 tctaaagctt ctggcatatc accaccacca cctgcttgct gtaatttaat aaaatttaac
     3781 atttttaata agtcgttatt aaaattgatg tgtttcgtta cataatcatc tcctctatct
     3841 ttataaaaaa cagccgatgc ctttaattct aaattattat atctgttaaa tgttttagtt
     3901 aatacatctt ctaattctaa tttcaaatat tcaatttcat ctcccatact tcctgttgca
     3961 tcaacaacaa atgcaatttc tacattgttc cttgctacac aacttttagt taaagtaatt
     4021 ctattaatac cttcatgaaa caagtacgga gttttaattt tcacactttc tccttcacaa
     4081 actattatat agttgttgtt ttgcgatgta gttgtattca ttctgcccca taactctgat
     4141 tttccagtat tatctgttat agcttgccaa acagtatcta aagtatttgc atctaataaa
     4201 taaacttttt gaccaattgc agcatagtaa ttatttgttt gcacttgcac acagtaacgt
     4261 ttttttgcaa atagtttcca gtaattagag taggttgcaa aatctttatc tgtataatct
     4321 tcccacattt tccatttaga aaaatcattt acctcgcctg ctgtaagtaa tttagtttcg
     4381 tattggtctt tggcaacttc aacgctatct tcataataat aagttttgct tttaccccca
     4441 tcagatgcag agcccatagc tcttgtactt ttaattttac cataactaac tgttgaaact
     4501 tctttgtgtt tagaggcatc ggaagttttt gtatatgtat cgctactact aactgtacct
     4561 gctgaaacta agcctctacc tgccattgtt aacatttcag ctccttctaa aaaacctgca
     4621 ccttctactg taggatcaga tgatttttct cttttagtta atagcacaat agaaaaatcg
     4681 ggatctttca ccgttttttc tgtaactata actttatagg attcatatcc ttctgcacta
     4741 aaaatcaatg tatcgggtaa tttggtagcc ataattttaa agctaccgtc tttatttgta
     4801 gatatagtat tggctttgtt cgtaaacttt acacttatat tggctgctgc cttgcctcgt
     4861 tcatttatta ctctaccatt tatcatttgg ctgtaagagg ttaaagaaaa agtaagcaac
     4921 aacagtacaa agtatatatt tttaaagttt ttcatactaa aattttatac cagatgcaaa
     4981 aaggttttta attacataaa atttgccaaa aaaatatttt atcatttctt tcaattataa
     5041 agtgccattt ttgctttaat aaaatttgaa ataaaccaca tgaaaaaagt aatacttttt
     5101 gtttttgttc taatcatttc agcaaaattc agtttttcgc aaaaaactgt tgccaatgga
     5161 tggcatttgt taaattacca gcaagatagt gtttatggta ttgatttaaa ccgagcctat
     5221 gcctttcttc ttgaaaaaaa taaaaaagca acacctgtta ttgttgcagt tatagactct
     5281 ggtattgata ctgcacatga agatttaaaa aatattttat ggaagaatac taaagaaatt
     5341 cctggcaatg gtattgatga cgataaaaat gggtatatag atgatattta tggttggaat
     5401 tttttaggca ataaagacgg tagaaatatt aaaaaagcca gtagtgaaaa gagtagaatt
     5461 tttcatactt ataaaaataa atatgaaaat aaagagttag ataccaattt gctttctaaa
     5521 gatgaaaaat acatttataa aacttggtta agaagtgcag cagaaattaa tgcaagtaca
     5581 caggagcagg caaatattac ctatatagaa cttgcattaa aaagcttaat taagaatgat
     5641 aaaatagtaa gagaagatat gggtaaagaa gaatatactt ttgaagaatt agaaatttat
     5701 atacccaaaa caaatgatgc taaaagagca aaagttggtg tattgcaaat aattaaactt
     5761 tttagcttag attctaaaag taaaaatact gaggtaattg aggaaataag tgaatatgta
     5821 gaaggcaaaa aagcatcttt tgaagaagca gaaaaagcac catttgatta tagagcagat
     5881 attgtaaaag ataatactaa agatattaat gataagaatt atggcaataa tgatgttatg
     5941 gcatctactc caaaacatgg aacacatgtt agtggcatta ttgctgcaca acgtaacaat
     6001 aacttaggta tggatggagt tgcagacaat gtaaaaatta tgacaattag agctgtgcca
     6061 gatggagatg aatacgataa agatgttgct ttagctattt tttatgcagt tgataatgga
     6121 gcaaaagtga ttaatatgag ttttggtaaa ggcttttcgc cagaaaaaaa atgggtggat
     6181 agtgcagtac aatatgctgc ctctaaagat gtaattatac tgcatgctgc aggcaacgat
     6241 tcaaaaaata ttgactcatc cgagaacttt ccaaatccat ttatgtatta tacaggtaaa
     6301 agagctccta actatgtaac tgttggagca agtagcgatc ctaaaatttc tggttcatta
     6361 gcagcaagtt ttagcaacta tggaataaac acagtagatg tttttgcccc tggcgataaa
     6421 atttattcta ctatacctgg tggtaataaa tatggcaatt tacaaggcac tagtatggca
     6481 acgcctgttg ttgctggtat tgcagcatta ttacgttctt attaccctag tttaagtgct
     6541 gttcaaatta aaaatattat tgaaaaatct gcaactattt acgataattc tgtagcaact
     6601 tttttgccag gtacaaatga taaaacaact ttaagtgagt tatgtagaac tggtggttta
     6661 gttaatgcct atgctgctgt tcaacttgca gaaaaaactg taccagaagt aaataaagta
     6721 gaagaaaata aattgcctat taaacctact aaaaaggtaa aaaagaaaaa ttaatggcta
     6781 ctcctaaaga acaagattat cctgcatatt atgcccaata tttagcaaaa ataaatgctg
     6841 caaatatttt ggatctaatg agcaaatatg caggtttaat tgaacaattt atacatgaaa
     6901 taccagatga taaagccaac tttgcttatg caaaaaacaa atggactatt aaagaagtat
     6961 tacaacattt aatagattca gaaagagttt ttgtgtatag ggcaatgcga tttgcaagaa
     7021 aagattttat tgaattagaa ggctacgatg aaaatgaata tactaaaaat gctcatgcaa
     7081 attgtagaga ctttagtatt ttaaaacaag agtttttatt actgaggaaa acaactgatt
     7141 tat
//
LOCUS       DAOQHP010000072         7030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5648_length_7030_cov_3.400860, whole genome shotgun sequence.
ACCESSION   DAOQHP010000072 DAOQHP010000000
VERSION     DAOQHP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_7030_cov_3.400860"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..302)
                     /locus_tag="PLY81_03595"
     CDS             complement(162..302)
                     /locus_tag="PLY81_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29341.1"
                     /translation="MEYRKTATKTKGKSVAKKTSTKQKGNVEAVLKAAKTNKLDLSYL
                     RE"
     gene            complement(759..1148)
                     /locus_tag="PLY81_03600"
     CDS             complement(759..1148)
                     /locus_tag="PLY81_03600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNF29342.1"
                     /translation="MSIFSKIIAGKIPCFKIAEDENFLAFLDIFPVQYGHTLIIPKIE
                     IDKLFDLPNEYLSTILVFAKPVAQAIQKAYPCNRVNIITVGLEVPHAHIHLIATNGLK
                     DVDLNKPKLKLTNEQLKEVQMNILKFL"
     gene            complement(1159..1413)
                     /locus_tag="PLY81_03605"
     CDS             complement(1159..1413)
                     /locus_tag="PLY81_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29343.1"
                     /translation="MLLKIILWGIVIYYLYKFVFGVVMPVSKATSDMKSKINEMQRAQ
                     EQFIKQQQQQQEQFQKQKVHEENIHLKKGDYIEFEEIKDK"
     gene            complement(1438..2331)
                     /gene="fabD"
                     /locus_tag="PLY81_03610"
     CDS             complement(1438..2331)
                     /gene="fabD"
                     /locus_tag="PLY81_03610"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNF29344.1"
                     /translation="MNKHAFIFPGQGSQFKGMGKNLYETNSLAKEMFEQANEILKFRI
                     SDIMFEGSDEALKQTNVTQPAVFLHSVIAYSTLHNAKPDMVAGHSLGEFSALVANKTL
                     SFEDALLLVSIRANAMQKACESNPSTMAAVLALADDKVEEICSLVQKQSGEVVVAANY
                     NCPGQLVISGSIKGIEIACEQMKAAGAKRALVLPVGGAFHSPLMSEAKAELAMAIEAT
                     IFNTPICPIYQNVVAKGISDATQIKQNLILQLTGAVKWTQSVQAMVADGATHFTEAGP
                     GKVLQGLVQKIYKEAMVDGVS"
     gene            complement(2341..3270)
                     /locus_tag="PLY81_03615"
     CDS             complement(2341..3270)
                     /locus_tag="PLY81_03615"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNF29345.1"
                     /translation="MSAFQFPNQTNFYQGKVRDVYTINNHFVVMIASNRISAFDVILP
                     KPIPFKGQVLNQIAATMLQATKDICPNWLINSPAPNVSIGKKCEPFKIEIVVRGNLTG
                     HAWRTYKSGQRILCGIKLPNGLRENDFFENPIITPTTKASVGHDEDISKDEIITQNLA
                     SLNEWETLEKYALQLFERGKQIAAKQGLILVDTKYEFGKINGEIFLMDEIHTPDSSRY
                     FYAEGFEERIENGQSQKQLSKEFVREWLLANNFMGKEGQTVPTMSNEWVQTISLRYIE
                     LYEKILGEKFKPQELSNEETYHKIEQSLNQLIN"
     gene            3428..6286
                     /locus_tag="PLY81_03620"
     CDS             3428..6286
                     /locus_tag="PLY81_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29346.1"
                     /translation="MLCIFTICAAAVSAQQTIISGKVSNSKSKESVSAVSVTVKGTTS
                     GTYTDDKGEFKLVTTQKLPFTLVFSSVGFADKEVSITDASQSVQVSLDVKYSMGDEIV
                     VAASRVSEKIIESPVSIERMGASAVKNAVAPNFYDAILNFKGVDMTTSSINFRTISTR
                     GFNGSGNLRANQLVDGMDNQAPALNFSVGNIVGMTELDVDNVELLPGASSALYGSGGM
                     NGTILMSSKNPFKYQGLSFQVKQGINHVDSKQTTAAPYFDWAFRWGKKVTERFAFKIS
                     GQFIQAQDWQAVDYANLARNNVYSQITNGDRITDPNYDGVNVYGDEVGANMQYLARLG
                     LLASGIAPNSAAYTGLNSVAGMGLNYAQSVAFIQNNVPALVPALAAFPTVFSINRGYY
                     ASNISRTGYNEKDLVDYNSYNVKLSGGLYYKLKNNVEASVVGYWGQATSVYTGIDRYS
                     LKNLKMGQYKAELKAKNWFLRGYLISENSGDSYANTITAVQMNRMWKADATWFSQYMT
                     AYTQTMLGTNGNATLAHAQGRAAADQGRYMPGSTGFMNAFEQVTGTSITNGGGLFADK
                     SKLYHFEGQYNLTDKIKVAEVLVGASYRVFNLNSGGTIFADHTPNFKNGNINISEVGG
                     YVQVAKRVNDFLKLTGSIRYDKNQNFDGRFTPRATALFKVAKDNNIRVSFQTAYRFPS
                     TQDQWINLNSPSAKLIGALPIFNDVYGFSTNPIYTAESVGLFRAKFAATGAIDPTVLV
                     KGTFQAVKPETVKSYEIGYRGVIKNKLMIDAYFYFSEYNNFLGRVAVARGQNGTQMEL
                     LSPSSSNNYSFVTNTASNVKAIGWGISADYMLPKGFVFNANVSGDQLNDVPSTVFTQF
                     NTPKMRFNIGLSNTNVYKNWGFGIVHRWQDKVNWEGTFGTGVIPSYGTWDAMVSYKTA
                     NKGLFKIGALNFWNKYYRSAFGNPQVGGVYYISYGYNL"
     gene            6510..>7030
                     /locus_tag="PLY81_03625"
     CDS             6510..>7030
                     /locus_tag="PLY81_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNF29347.1"
                     /translation="MTTNMLFLIVPILGIVGLLYTFIKFNWVAKQDAGNERMKEISNY
                     IAEGAMAFLKAEWKILTYFVIIVALLLGFMATKNPHSHWSIAIAFILGAICSAVAGYI
                     GMKVATKANVRTAQAARTSLSKALSVSFTGGAVMGVGVAGLAVLGLGGVFIILKGMFA
                     PEAVNSLEMLKTI"
BASE COUNT         2268 a         1098 c         1143 g         2521 t
ORIGIN      
        1 gcgggttcgt tttttttcat tttcacttgc accactaagt aattctaaat aattaatacg
       61 tgtaattagt atttgcgtta ttcttagttc attaaaaata gcttccattt ttgtgttgtg
      121 gtaattcaaa tggaaaagta ctgtagtatc tgcaattact atcattctct taaataagat
      181 aaatctaatt tatttgtttt tgcagctttt aaaacagctt caacatttcc tttttgcttt
      241 gttgatgttt ttttggcaac agactttcct ttggtttttg ttgctgtttt tctgtattcc
      301 ataaaaagag ttttaacaaa tgtacagaat ttaaggcttg ccattttttc tattgtttaa
      361 ataatttgtt tgtcttctga gttatatttt attaattcta ctttccaaat ttttaaacta
      421 aaattgctac ctgtaaatct gtgttgttac ttgcttaaga tttaactgct ttaatgtatt
      481 tcctaagcct tttgattggg tttaaatact tgttacctca tttagttttt tttcgttttt
      541 aagttttgtt tttataacat catccagtac ttttttgctg aggtgccata ttaataatat
      601 ttattaatta caaaaggtaa ataaaagttg gcaatattgt gccaacttct tagggataaa
      661 aacgtgtgac atttcatttt tacagcaaaa gttggtaatt ttctcttact gttcctactc
      721 cgtaggcctg gaacgtgtac gtcaccatgg tagcagatct acaaaaactt taaaatattc
      781 atttgaactt ccttcaactg ctcattggtt agttttaatt ttggcttgtt aagatcaaca
      841 tcttttaaac catttgttgc aattaaatga atgtgtgcat gtggcacttc taaaccaaca
      901 gtaataatat ttactctgtt acaaggatat gctttttgaa tagcttgtgc aactggtttt
      961 gcaaaaacta aaattgtaga aagatattca ttgggtaggt caaataattt atcaatttca
     1021 atttttggaa taattaaagt atgaccatat tgcacaggaa aaatatctaa gaatgctaaa
     1081 aaattttcat cttctgctat tttaaagcat ggaattttgc cggcaataat tttagaaaaa
     1141 atactcataa tatatctttt atttatcttt tatttcttca aattctatat agtctccttt
     1201 ttttagatgt atattttctt catgtacttt ttgcttttga aattgctctt gctgttgttg
     1261 ctgttgcttt ataaattgtt cttgtgctct ttgcatttca tttattttag acttcatatc
     1321 actagtagcc ttactaacag gcatcacaac accaaaaaca aatttgtata aataataaat
     1381 tactataccc cataaaataa tcttaagtaa catactttgt atttttttaa actacactta
     1441 actaacgcca tctaccatag cctctttata aattttttga accaatcctt gtaatacttt
     1501 tccagggcca gcttctgtaa aatgagttgc tccatctgct accattgctt gtacactttg
     1561 tgtccattta acagcaccag ttaattgtag tattaagttt tgttttattt gtgtagcatc
     1621 gcttatccct tttgctacaa cattttgata aattgggcat attggtgtat taaaaatggt
     1681 ggcttcaata gccatagcta attctgcttt tgcttctgac attaatgggc tatgaaatgc
     1741 accaccaacg ggtaatacta aagctctttt agcaccagca gctttcattt gttcgcaagc
     1801 tatttcaata cccttaatgc ttccactaat tactaactgt cctgggcaat tatagtttgc
     1861 tgcaactaca acttcgccag attgcttttg tactaaactg caaatttctt ctaccttatc
     1921 atctgcaagt gctaatactg cagccattgt acttgggttg ctttcacaag ctttttgcat
     1981 tgcatttgcc cttatactaa ctagtaatag agcatcttca aaacttaaag ttttattagc
     2041 taccaaggcc gaaaactcac ctaaagaatg ccctgcaacc atatctggct ttgcattatg
     2101 taatgtgcta tatgctatta ctgagtgtag gaaaactgca ggttgtgtaa cattagtttg
     2161 ctttagagct tcatcgcttc cttcaaacat aatatcacta attctgaact ttaaaatttc
     2221 atttgcttgt tcaaacattt cttttgccaa actatttgtt tcgtacaaat ttttacccat
     2281 tcctttaaat tgagagccct gacctggaaa aataaaagca tgtttgttca taattaattt
     2341 ttaattgatt aattggttta aagattgttc aattttatga taagtttctt cattagatag
     2401 ctcttgaggt ttaaatttct ctcctaaaat tttttcgtac aattcaatat aacgtagaga
     2461 aatggtttga acccactcgt tactcattgt tggaactgtt tgtccctctt tacccataaa
     2521 gttattagct aaaagccatt ctcttacaaa ctctttgctc agttgctttt ggctttgtcc
     2581 attttcaatt ctttcttcaa acccttcagc ataaaaatat ctagatgaat cgggtgtatg
     2641 aatttcatcc attaaaaaaa tttctccatt tatttttcca aactcatact ttgtatccac
     2701 taaaattaag ccttgtttag cagcaatttg ttttcctctt tcaaataatt gtaatgcata
     2761 tttttcaaga gtttcccact catttaatga agctaaattt tgcgtaataa tttcatcttt
     2821 actaatatct tcatcatgcc ctacacttgc tttagtggtt ggggtgataa ttgggttttc
     2881 gaaaaaatca ttttctctta aaccatttgg taacttaatg ccacacaaaa ttctttggcc
     2941 acttttataa gtacgccatg catgaccagt taaattacct cttacaacta tttctatttt
     3001 aaaaggctca catttttttc ctatagaaac atttggggca ggagaattta ttaaccaatt
     3061 ggggcaaatg tctttagtag cctgcaacat agtagcagca atttgattaa gtacctggcc
     3121 tttaaatgga attggttttg gtaaaataac atcaaaagca gaaattcgat tactggcaat
     3181 cattactaca aaatgattat taatagtata aacatctctt actttacctt ggtaaaagtt
     3241 tgtttgattt ggaaattgaa atgcagacat acattcaaaa ataacacaca aaaacttgtt
     3301 actaaaacta atttgaaaaa acaaataata attttcaaaa ataaatattt gtatagtaaa
     3361 ctaactgtta ttatattgct aaacaattaa acaatattgt agtatgagaa atatcaaatt
     3421 taccctaatg ctgtgcattt ttactatttg tgcagcagca gtttcggcac aacaaactat
     3481 tatttcagga aaagtttcca acagcaaatc aaaagaatct gtttcggcag tatcggttac
     3541 agttaaagga accacatctg gaacatatac agatgacaaa ggagaattta aattagtaac
     3601 tactcaaaaa cttcctttta ctttggtttt ttcatctgta ggctttgcag ataaagaagt
     3661 ttcaattaca gatgcttcac aaagtgttca agttagttta gatgtaaaat actccatggg
     3721 agatgagatt gttgttgcag catctcgtgt atcagaaaaa attatagaat ctccagtatc
     3781 tattgaacgt atgggtgcta gtgcagttaa aaatgcagtt gctcctaact tttacgatgc
     3841 tattttaaac tttaaaggtg ttgatatgac aacatctagt attaacttta gaactatcag
     3901 tacaagaggg tttaatggta gtggtaattt acgtgccaat caattagttg atggtatgga
     3961 taatcaagcc ccagctttaa atttctcagt aggtaatatt gttggtatga cagaattaga
     4021 tgtagataat gtagagttat tacctggtgc ttcttctgct ttatatggct ctggtggtat
     4081 gaatggcacc attttaatga gcagtaaaaa tccttttaaa tatcaaggat taagttttca
     4141 agtaaaacaa ggaattaacc atgtggatag caaacaaact acagcagctc cttattttga
     4201 ttgggctttt cgttggggta aaaaagtaac agaaaggttt gcatttaaaa tttctggaca
     4261 atttatacaa gcccaagatt ggcaagcagt agattatgcc aacttagcta gaaataatgt
     4321 gtacagccaa attaccaatg gagacagaat tactgatcct aattatgatg gtgtaaatgt
     4381 gtatggagat gaagttggag caaacatgca atatttagca aggttaggtt tattagcaag
     4441 tggtattgca ccaaattctg ctgcatatac tggcttaaac tcagttgcag gtatgggatt
     4501 aaattatgct caatctgttg catttattca aaataatgta ccagctttag taccagcttt
     4561 ggctgcattc ccaactgttt tttctatcaa tagaggatat tatgcttcta atattagccg
     4621 tacaggatat aacgaaaaag atttagttga ttacaattct tacaatgtaa aactttccgg
     4681 aggtttatat tacaaactta aaaataatgt tgaagcttct gtagtaggtt attggggaca
     4741 agctacaagt gtttacacag gtattgatag atatagctta aagaatttaa aaatgggtca
     4801 gtataaagca gaattaaaag ctaaaaattg gtttttaaga ggctacttaa tttctgaaaa
     4861 ttctggcgat tcttatgcaa acactattac tgcagttcaa atgaacagaa tgtggaaagc
     4921 tgatgctact tggttttctc aatacatgac tgcttataca caaacaatgt taggtaccaa
     4981 tggaaatgcc actttagctc atgcacaagg acgtgcagct gcagaccaag gaagatatat
     5041 gccaggttct acaggcttta tgaatgcgtt tgaacaagta acaggaactt caattactaa
     5101 tggtggaggc ttatttgcag ataaaagtaa actataccat tttgaaggcc agtacaattt
     5161 aaccgataaa ataaaagtgg cagaagtatt ggttggtgca agctacagag tatttaacct
     5221 aaattctggt ggtactattt ttgctgacca tacacctaat tttaaaaatg gaaatattaa
     5281 tattagcgaa gtaggtgggt atgttcaagt tgctaaaaga gtaaatgatt tcttaaaact
     5341 tacaggttct atcaggtatg ataaaaacca aaactttgat ggtagattta caccaagagc
     5401 aactgcttta ttcaaagttg caaaagacaa taacataaga gtatcctttc aaacagctta
     5461 tcgttttcct tctacacaag accaatggat taacctaaac agcccaagtg caaaattaat
     5521 tggtgcatta ccaatattta atgatgtata tggctttagt acaaatccta tttatacagc
     5581 ggaaagtgtt ggtttattcc gtgctaaatt tgcagcaact ggtgcaattg acccaacagt
     5641 acttgttaaa ggtactttcc aagcagttaa accagaaact gtaaaatcat acgaaattgg
     5701 ctacagagga gttattaaaa ataaattaat gatagatgct tatttctatt ttagtgaata
     5761 caataacttt ttaggaaggg tagcagtagc aagaggtcaa aatggtactc aaatggaact
     5821 tttaagccca tcaagcagca ataattattc ttttgttact aatactgcta gcaatgtaaa
     5881 agcaattgga tggggtatta gtgctgatta tatgttacca aaaggatttg tatttaatgc
     5941 aaacgtttct ggcgaccaat taaatgatgt tccttctaca gtatttaccc aatttaatac
     6001 tcctaaaatg agatttaata ttggcttaag taacaccaat gtatataaaa attggggctt
     6061 tggtattgtg cacagatggc aagataaagt gaattgggaa ggtacttttg gtacaggtgt
     6121 tattccttct tatggcacat gggatgctat ggttagctac aaaacagcta acaaaggatt
     6181 atttaaaatt ggtgctttaa acttctggaa taagtattat agaagtgcat ttggtaaccc
     6241 tcaagtaggt ggtgtttact atataagtta cggttataac ttataattaa tacaacatat
     6301 taattactaa aggctaccct ttttaagggt agccttttta atataatttt aaaagggtag
     6361 aacaaatgga acaactgaat ttattttata caatactaaa atttgaaatt aataaaaacc
     6421 ttatgaatta gttcatttaa actgatattt ataataaata tccccctatt tttgccaccc
     6481 attttaaatt tttaagtata tacttgctta tgactactaa catgttattt ttaatagtac
     6541 ctattttagg tattgttggt ttgctttaca catttattaa gtttaactgg gttgccaaac
     6601 aagatgctgg caacgaaaga atgaaagaaa ttagtaatta cattgcagaa ggtgctatgg
     6661 catttttaaa agcagagtgg aaaatactaa cctattttgt aattattgta gccttgttat
     6721 taggctttat ggctactaaa aatccacata gccattggag tattgcaata gcctttattt
     6781 taggagcaat ttgtagtgca gttgcaggtt atataggtat gaaggttgct acaaaagcca
     6841 atgtacgtac agcacaggca gctcgtacaa gtttatcaaa agctttatct gtatcattta
     6901 caggaggagc agtaatgggt gttggtgtag caggtttagc tgttttagga ttaggaggtg
     6961 tatttattat attaaaaggc atgtttgcac cagaggctgt aaactcatta gaaatgctta
     7021 aaactattga
//
LOCUS       DAOQHP010000073         6982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5731_length_6982_cov_3.920167, whole genome shotgun sequence.
ACCESSION   DAOQHP010000073 DAOQHP010000000
VERSION     DAOQHP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_6982_cov_3.920167"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLY81_03630"
     CDS             complement(<1..254)
                     /locus_tag="PLY81_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29348.1"
                     /translation="MIKKKLILLMWIAVGILTVVLLGAAMQKKNEYKCKDIKIEIVGS
                     VQHMFVDEKDILQTVNSIGLVTGNSISNINISKVYIYCLPA"
     gene            complement(255..1610)
                     /gene="murC"
                     /locus_tag="PLY81_03635"
     CDS             complement(255..1610)
                     /gene="murC"
                     /locus_tag="PLY81_03635"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNF29349.1"
                     /translation="MIELDNIKKIYFIGIGGIGMSALARYFKFKKVEVAGYDKTKTAL
                     TQLLENTGINIHYSEDVDAIPKDVDVVVYTPAIPNEHKELVYYQQHQYHVVKRSDILQ
                     CITESSFNICIAGTHGKTTVTTMIAHVLRHSGFGCNAFIGGIASNYNTNFWSSENNVV
                     VVEADEYDRSFLKLVPDVAVITAMDADHLDIYGTAQELEKTFIQFSKKLKQNGCLITK
                     HGLKRDTELNADTKFTYSFQNNLASVYAGNIRVVNGSYVFDVIGNQFNINNITLHLGG
                     LHNIENAIAAITVATYLKIDDSKIKDAIEDFKGVKRRFEYIVRNEENVLIDDYAHHPE
                     ELKALINGVRSIYKNEKLTIVFQPHLYSRTNDLAAGFAESLDLADEVILLPIYPAREL
                     PIAGVESEMILSKMNLKNKQVLSKNDFINWVKNNKPKMLVMAGAGDIDVMVNDVKEIL
                     A"
     gene            complement(1607..2704)
                     /gene="murG"
                     /locus_tag="PLY81_03640"
     CDS             complement(1607..2704)
                     /gene="murG"
                     /locus_tag="PLY81_03640"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNF29350.1"
                     /translation="MIEATKIIIAGGGTGGHIFPAIAIANALKLHQPNIQILFVGAKG
                     KMEMEKIPQAGYEIKGLDIAGFNRSSLIKNIGLPFKLIKSFIQVRSIFKQFKPHAVVG
                     VGGYSTFPVLKYAQAKSIPTFIHESNSFAGKANRMLGKNATKIFVATSGMEQFFTKDK
                     IFISGNPVRKSIVENSITKQEALKFFNLREEKKTVLVIGGSLGAKSINQAIAMQINEF
                     AKNNLQLIWQTGKLTADEYLAIAKGKSNIWCNHFINTMEMAYAAADIVISRAGAMAVA
                     ELCISAKPVVFVPFPFAAEDHQTANAKVLVNKQAALMIQDNEVKEKLVETVFELAKND
                     EKMQSMRTNIASLAIKNADEIIASEILKNIK"
     gene            complement(2805..4142)
                     /locus_tag="PLY81_03645"
     CDS             complement(2805..4142)
                     /locus_tag="PLY81_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNF29351.1"
                     /translation="MFNKSNIPFSKTSPLSKLSKADIPFASLNKESLLKRTSGDKYIW
                     GIVLVLALISILVVYSATGSLAYKMNKGNNEVYLFKQLSFTVIGLMVMYFLHRINYTL
                     FSRIATLLFFISIPLLIYTLVFGAKINEGSRWIKLPVINMTVQTSDIAKLALFMYISK
                     LLSKKQEVIKDFKKGFLPIITPVFVICVLIMPANLSTALLTGATSLLIMFIGRISLKH
                     ILLTILVASIPILFIVSVAWLTYNKSDVNERTKPVVTEKLKSVGRFGTWVKRVQDHLF
                     AVDTEVPYQVQQAKIAIAKGGILIGQGPGNSEQRNFLPQAYNDFIYSIIIEEYGLLGA
                     AVIIFIYLSFLFRCIKIFRRCPYAFGAFLALALSFTLVIQAIANMAVNVNLLPVTGVT
                     LPLVSMGGSSFLFTCAAIGIILSVARNVEQLEGVKDEQDRTNNFNGQQQAVVE"
     gene            complement(4186..5526)
                     /gene="murD"
                     /locus_tag="PLY81_03650"
     CDS             complement(4186..5526)
                     /gene="murD"
                     /locus_tag="PLY81_03650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNF29352.1"
                     /translation="MAHRIVILGAGESGVGAALLSKKKGYEVFVSDAGVIKEKYKKKL
                     FDNEIEFEEGKHTAEIILNANEVLKSPGIPETNKMVIAIREKGIEVIGEFELAYRCKG
                     DSKIVAITGSNGKSTTTALIYHICQKAGLDCALVGNIGYSIAAQIAEDPKMLYVAEIS
                     SFQLDDIKTFRPDIAVLLNITEDHLDRYNYQFENYINSKFRVILNQNKNDYFIYNDDD
                     EIITKKLKTLIPNTNPLPFSMKHEIKKGGNIKGDQMMLRIQEERVSMSIYDFALKGKH
                     NNYNTMAAGIAAATLGIRKDKIREAVKDFQSLEHRMEFVATVRGVEFINDSKSTNTNS
                     TWFALESMNKPTVLILGGVDKGNDYELMNELVAQKVKAIVCLGNDNKKILEAFKDKVP
                     VIVEADSAKKAVNAAFKLSVKGDVVLLSPACASFDLFKNYEDRGVQFKEAVKEL"
     gene            complement(5608..6915)
                     /gene="mraY"
                     /locus_tag="PLY81_03655"
     CDS             complement(5608..6915)
                     /gene="mraY"
                     /locus_tag="PLY81_03655"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNF29353.1"
                     /translation="MLYHLFTWLKNEFNLPGSGLFQFLTFRIAMAVLLSLLIATVYGK
                     RIIIYLQKKQIGESVRDLGLEGQMQKKGTPTMGGIIILLSILIPTLLFANLDKVYIRL
                     MILCTVWLGIIGFLDDLLKIRARKAAQQKGEAYKKKDSDGLAGISKIIGQIGLGIIIG
                     ATLYFSNAVTVEREVIGTTYQKQAEEKFAKDSTIFSRNIDGVEKRYVTVKTPITTIPF
                     VKNHEFNYSKLISWIGSGAEKYTWIVYIFIVTLIITAVSNGANLTDGLDGLAAGVSAI
                     IGIGIGLFVYVSGNYKFADYLNIMYIPNLGELSIFVAAFVGACIGFLWYNAYPAQVFM
                     GDTGSLALGGIIASLAIIVRKELLIPIFCGIFLVENLSVIIQVSYFKYTKKKYGEGRR
                     VFLMSPLHHHYQKRGYHENKIAIRFWIITVLLVIASIATLKMR"
BASE COUNT         2201 a         1328 c          870 g         2583 t
ORIGIN      
        1 gcgggcaaac agtaaatata aacctttgaa atgttaatat tactaattga attgcctgtt
       61 actaaaccaa ttgaattaac tgtttgtaga atatcttttt cgtcaacaaa catgtgctgt
      121 actgaaccaa caatttcaat tttgatatcc ttgcacttgt actcattctt tttttgcatg
      181 gctgcaccaa gtaatactac agttagtatg cctactgcaa tccacattaa taatatgagt
      241 ttctttttta tcacttatgc taaaatttct tttacatcat ttaccattac atcaatatct
      301 cctgcacctg ccattaccaa catttttggc ttattatttt tcacccagtt tataaaatca
      361 ttttttgata acacttgttt atttttcaaa ttcattttgc tcaaaatcat ttcactttca
      421 acccctgcaa ttggcaattc tctagctgga taaatgggta ataatattac ttcatctgcc
      481 aggtctaagc tttctgcaaa acctgctgcc aaatcatttg ttctgctata taaatgaggt
      541 tgaaatacta tagttaattt ttcattttta taaatacttc taactccatt tatcaatgcc
      601 tttaattctt ctggatgatg agcataatca tcaattaaaa cattctcttc atttctaact
      661 atatattcaa atcttctttt aactccttta aaatcttcaa ttgcatcttt tattttacta
      721 tcatcaatct ttaaatatgt agcaactgta attgctgcaa ttgcattttc tatattatgc
      781 aagccaccca aatgaagtgt tatattatta atattaaatt gattgcctat aacatcaaaa
      841 acataactac catttactac tctaatatta cctgcatata cacttgctaa attgttttga
      901 aagctgtatg taaattttgt atcagcattc aactcagtat ctctttttaa cccatgttta
      961 gttattaagc agccattctg ttttaacttt tttgaaaact gaataaatgt tttttccaac
     1021 tcttgggcag ttccataaat atctaaatgg tctgcatcca tagcagtgat aactgcaaca
     1081 tctggtacca gttttaaaaa actcctatca tattcatctg cctctaccac cactacatta
     1141 ttttcactac tccaaaagtt ggtattgtaa ttgcttgcaa tacctccaat aaaagcattg
     1201 catccaaaac cgctatgtct taaaacatga gcaatcatag tagttacagt tgttttacca
     1261 tgcgtgcctg caatacatat attaaaagaa ctttcagtga tacattgtaa aatatcgcta
     1321 cgttttacaa catggtattg gtgttgttga taatacacta attctttatg ctcgtttgga
     1381 attgcaggtg tataaaccac tacatccaca tctttgggaa ttgcatctac atcttcggag
     1441 taatgaatgt ttatgcctgt attttcaaga agttgggtta gtgcagtttt tgttttatca
     1501 taccctgcta cttcaacctt tttaaattta aaataccttg ctaaagcact cataccaatg
     1561 cctccaattc caataaaata aattttcttt atgttatcta attctatcat ttaatatttt
     1621 tcaaaatttc tgatgcaata atttcatccg catttttaat tgctaacgat gcaatattgg
     1681 tacgcataga ttgcatcttt tcatcatttt ttgctaattc aaaaacagtt tctactaatt
     1741 tttctttaac ctcattatcc tgtatcatta atgctgcctg tttattcact aaaacttttg
     1801 catttgcagt ttgatgatct tctgcagcaa aaggaaatgg cacaaacact acaggctttg
     1861 cagaaataca taattctgca actgccatag ctcctgcacg gctaattaca atatctgctg
     1921 ctgcataagc catttccatt gtattaataa aatggttaca ccaaatattg cttttacctt
     1981 ttgcaatagc taaatactca tcggctgtta actttccggt ttgccaaata agctgcaaat
     2041 tattttttgc aaactcgttt atttgcatag caatagcttg attaatgctt tttgcaccca
     2101 agctgccacc aattaccaat accgtttttt tctcttctct taaattaaaa aactttaatg
     2161 cctcttgttt agttattgaa ttttctacaa ttgattttcg tacaggatta ccagaaataa
     2221 aaattttatc ttttgtaaaa aattgttcca tgcccgaagt agccacaaaa atttttgtag
     2281 catttttacc cagcattcta tttgctttac cagcaaaaga gttactttca tgaataaatg
     2341 taggtattga ttttgcttgg gcatatttaa gtactggaaa tgttgaataa ccaccaacgc
     2401 caactactgc atgtggctta aactgtttaa atatgctgcg tacctgtata aaacttttta
     2461 taagtttaaa aggcaaacca atatttttta tcaaagaact tctattaaaa ccagctatat
     2521 ctaatccttt tatttcatat cctgcctgag gaattttttc catttccatt ttcccttttg
     2581 caccaacaaa caaaatttga atgtttggtt gatggagctt tagtgcattt gcaatagcaa
     2641 ttgcaggaaa aatatgacct cctgttcctc caccagctat aataatttta gtagcttcta
     2701 tcatagttgg ctagttatat ttttcatttt gttccacttt ctaaaaaaat tatttctttc
     2761 ttctttcatt tctaaaccaa tccacatctt taaccaatta cttcttattc aaccacagct
     2821 tgctgttggc cattaaaatt atttgttctg tcttgttcat cttttactcc ttctaattgt
     2881 tctacatttc tagcaacact taatataata ccaattgcag cacatgtaaa taaaaaggaa
     2941 cttccaccca tacttaccaa tggtagtgta actcctgtaa caggtaacag gtttacatta
     3001 acagccatat tggctattgc ttgtattacc aatgtaaagc ttaaagcaag tgctaaaaat
     3061 gcaccaaatg cataagggca tcttctaaaa attttgatac atctaaacaa aaaagacaaa
     3121 taaataaata tgataacggc agcaccaagt aaaccatatt cttcaataat tattgaataa
     3181 ataaagtcgt tataagcttg tggtaaaaaa tttctttgct cactattacc tgggccctga
     3241 ccaattaaaa tgcccccctt tgcaatagct attttagctt gttgtacttg gtatggaact
     3301 tctgtatcaa ccgcaaacaa gtggtcttgt acacgtttaa cccaagttcc aaaacgccca
     3361 accgatttta atttttctgt tacaaccggt ttagttcttt catttacatc tgatttattg
     3421 taggtaagcc aagcaacaga tactataaat aatattggta tagatgcaac taatatggtt
     3481 agtaaaatat gctttaaact aattctacct ataaacataa ttaataaaga agttgcccct
     3541 gtaagcaaag ctgtacttaa gtttgcaggc attattaaaa cacaaattac aaacactggt
     3601 gtgataattg gcagaaaccc tttcttaaaa tcttttatta cttcttgctt tttgcttaat
     3661 agctttgaaa tatacataaa caaagcaagt ttagcaatgt cgctggtttg aacagtcata
     3721 ttaattacag gtagctttat ccacctacta ccttcattaa tttttgctcc aaaaactaat
     3781 gtataaatta acagaggaat agagataaag aataacaaag ttgcaattct tgaaaaaagc
     3841 gtataattta ttctatgcaa gaaatacatt accatcaacc ctattactgt aaaggataac
     3901 tgtttaaaca aatacacttc attattgcct ttgttcattt tatatgccaa agatccggtt
     3961 gcactataaa ccacaagaat ggaaataagt gctaatacca atacaattcc ccaaatgtat
     4021 ttgtctccac tagttctttt tagtaaactt tctttgttta gtgaagcaaa tggaatatct
     4081 gctttgctta atttagaaag tggagaggtt ttagaaaatg gtatgttaga cttattaaac
     4141 attaattctt gttattctat ttttgctttt attgtttgat aaatactata gctcttttac
     4201 agcttcttta aattgaactc ctctgtcttc gtagttttta aacaaatcaa agcttgcaca
     4261 tgcaggactt aataaaacta catcgccttt tacactaagc ttaaatgcag cattaacagc
     4321 tttctttgct gagtctgcct ctactataac aggtacttta tctttaaatg cctccaaaat
     4381 ttttttatta tcgttgccta agcaaacaat agcttttact ttttgggcta ctaattcatt
     4441 cattaattcg tagtcattgc ctttatcaac accacctaaa ataagtactg ttggtttgtt
     4501 catactttct aaagcaaacc aggtactgtt ggtatttgtt gacttactat cattgataaa
     4561 ctctacacca cgaacagtag caacaaactc catacgatgc tcaaggcttt gaaaatcttt
     4621 tacagcttct cttattttat cttttctaat tccgagtgta gctgctgcaa tacctgctgc
     4681 catggtgtta tagttgttgt gtttaccttt tagagcaaag tcgtaaatac tcatacttac
     4741 tctttcctcc tgaatgcgga gcatcatttg atctccttta atgttgcctc ctttttttat
     4801 ttcatgtttc atagaaaatg gtaatgggtt agtatttggg attaatgttt ttaatttttt
     4861 ggttattatt tcatcatcat cattataaat aaaataatca tttttgtttt gattcaagat
     4921 gactcgaaac ttactgttta tatagttttc aaactgatag ttatatctgt ctaaatggtc
     4981 ttctgtaatg tttagtaaca cagctatatc tggcctgaaa gttttaatat catctaactg
     5041 aaatgaactg atttcggcta catacaacat tttaggatct tctgcaattt gtgctgcaat
     5101 gctgtaacca atgttaccaa ccaatgcaca atccaatcct gccttttggc agatatgata
     5161 aataagtgca gttgttgtgc ttttaccatt gctacctgta attgcaacta ttttgctatc
     5221 tcctttacat ctatatgcca actcaaattc accaattact tctattcctt tttctctaat
     5281 agcaataacc attttattag tttcaggaat tcccgggctt ttcaaaactt cgttggcatt
     5341 taatataatt tcagcagtat gttttccttc ttcaaattct atttcattat caaagagctt
     5401 ttttttatat ttttctttta ttactccagc atcgctaaca aaaacttcgt atcctttctt
     5461 tttacttaat aatgcagcac ctacaccact ttctcctgcc ccaagtatta ctattctatg
     5521 tgccatattt ttttaaattc tctttaatat ttttctttca acaagcagta aattttaact
     5581 cctactttaa aagttcactt acagctttta ccgcattttt aatgttgcta tactagcaat
     5641 aactaataac acagtaatta tccaaaacct tattgcaatt ttattttcgt gataccctct
     5701 tttctgataa tgatgatgta aagggctcat taaaaaaacc cttcttcctt ctccgtattt
     5761 ctttttggta tatttgaaat aagaaacctg aatgattacg cttaaatttt caacaagaaa
     5821 tattccgcag aatattggaa ttaataattc ttttcttaca ataatggcta atgaagcaat
     5881 gatgccacct aaagccaaac taccagtatc gcccatgaaa acttgtgcag gataagcatt
     5941 gtaccacaaa aatccgatac atgcaccaac aaatgctgca acaaaaatgc ttagttcacc
     6001 caaattgggt atatacataa tattcaaata atcggcaaac ttgtaattac cgcttacata
     6061 tacaaacaag ccaataccaa taccaatgat tgcacttaca cctgcagcca aaccatctaa
     6121 accatctgtt aaatttgcac cattactaac agctgttatg atgagtgtaa caataaaaat
     6181 gtacactatc caagtatatt tttcagcacc acttcctatc cagctaatta atttactgta
     6241 attaaattca tgatttttaa caaaaggtat agttgtgatt ggtgttttaa cagtaacata
     6301 tcttttctct acaccatcaa tatttctaga aaatatagtg gagtcttttg caaatttttc
     6361 ttctgcttgt ttttgatagg ttgtgcctat tacttcacgt tctacagtta ctgcattact
     6421 aaaatataat gtggcaccaa taataatacc taaaccaatt tgaccaataa ttttacttat
     6481 acctgctagg ccatcactgt ctttcttttt ataagcttcg cctttttgct gagcagcttt
     6541 tcttgctctt atttttaata aatcgtctaa aaagccaata ataccaagcc acacagtgca
     6601 taaaatcatt aatcgtatat acactttatc taagtttgca aataacaatg ttggaattaa
     6661 aatgcttaat aaaataatta ttccacccat tgttggcgta cctttttttt gcatttgacc
     6721 ttcaaggcca aggtctctca cactctctcc aatttgcttt ttttgtaagt aaataataat
     6781 tcgtttacca tatacagtag ctataagcaa cgataaaagc actgccattg caatacgaaa
     6841 cgtaagaaac tgaaacaagc cagagcctgg taagttaaat tcgtttttta gccatgtaaa
     6901 taaatggtat agcataaatt gattttctac tattgttgtt tattcaataa attgtttaat
     6961 atttctttat catcaaaagg gt
//
LOCUS       DAOQHP010000074         6879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5842_length_6879_cov_3.657972, whole genome shotgun sequence.
ACCESSION   DAOQHP010000074 DAOQHP010000000
VERSION     DAOQHP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5842_length_6879_cov_3.657972"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLY81_03660"
     CDS             <1..1025
                     /locus_tag="PLY81_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNF29354.1"
                     /translation="EIHVSGLFPQALPFICFLAFWFLNMLIIYYGVESIKKLLVFKAI
                     FLPVATLALLFWAITQANGLDNILNQESKLHGSDFWKYFFPALTGMIGFWATLSLNIT
                     DFTRYAKSQKAQITGQAIGLPPSMTLFAFVGVVVTLATTIIYGQTEWDIVKLASKFES
                     KLVVSFAMIGIIISTLATNIAANIVSPANDFANLNPKKINFKIGGYITGVVGILILPW
                     KLIADPNGYIFTWLIAYSGLLGPIGGIMIADYFFIRKQKLIVEDLYKHNGMYGYKNGF
                     NIIALIAFIIGVIPVVPGFLVTVKLIDPNSVATWLSILYHYAWFVGFFVSGILYLILT
                     AKNAKD"
     gene            1048..1425
                     /locus_tag="PLY81_03665"
     CDS             1048..1425
                     /locus_tag="PLY81_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF29355.1"
                     /translation="MTENEISNKVIGNGIEVHSALGPGLLESAYKECLYYKLISSGFL
                     VEKEKAMPLVFENVKLECGYRIDLLVEKKLVIEIKSVEALNDIHLAQTLTYMKLGNYK
                     LGLLMNFNVLRLKDGIKRVVNGL"
     gene            1481..2863
                     /gene="hydA"
                     /locus_tag="PLY81_03670"
     CDS             1481..2863
                     /gene="hydA"
                     /locus_tag="PLY81_03670"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNF29356.1"
                     /translation="MSVLIKNGRIITADSDQTADIFISGETVQAIGKNLNVQSDKEID
                     ATGLLIFPGGIDPHVHLDMPFMGTFSSDSYETGTRAALYGGTTMVLDFILQKQGNSLQ
                     DALQEWKSRSDNNCVGDYSFHMAVTDFNEETKKEIKHFIEEEGITSFKTFMAYKGALM
                     IDDRQMIGLMEEVKKHGGLINVHATNGDMIDYLIAKHKEEGKLSPLYHYLSQPEITEA
                     EASERFIDMASYTGCPGYIVHLTCEGALNAVRNATKRNQKIFVETCIQYLILDASLYE
                     RDFEGAKWVMSPPLREKKDQQTLWAGINQGLVQVVATDHCPFMWEQKLLGKNDFSKIP
                     NGHPAIENRMELLFSEGVEKNKITLNKFVEVTSTNAAKIFGMFPKKGTIAVGSDADIV
                     LFNPNEKHVLSASTHHMNVDYSGYEGWEVTGKVKTVLLRGKIAIDNNECKVEKGFGKF
                     VKRNKVASIV"
     gene            2889..3269
                     /locus_tag="PLY81_03675"
     CDS             2889..3269
                     /locus_tag="PLY81_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF29357.1"
                     /translation="MSDLKKTDSIIQQKSYAFALAVIALYKNLVTDKKEFVLSKQVLR
                     SGTSIGANIQEAVASQSKRDFIHKLSISLKEARETQYWLNLLKDSSYIPESEFNNLNN
                     SCDEVKKILTSIILTTKERYLSNS"
     gene            3363..4229
                     /locus_tag="PLY81_03680"
     CDS             3363..4229
                     /locus_tag="PLY81_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNF29358.1"
                     /translation="MPRIIKSGLIQMSLPKTEGEGTIEEIKEAMVQKHIPLIEEAGKK
                     GVQILCFQEIFNTPYFCPGQDANWYASAETVPGPTIERMQVYAKKYNMVIIVPVYEKE
                     QAGVLYNTAAVIDADGSYLGKYRKNHIPHTGGFWEKFFFKPGNLGYPVFQTKYAKVGV
                     YICYDRHFPDGARCLGLNGAEIVYNPSATTVGQSQYLWKLEQPAHAVANGYFMGCINR
                     VGTEKPWNLGKFYGTSYFVNPLGQIIAEASEDKDELLIAEFDLDLIEQIRSKWQFFRD
                     RRPETYGELVRL"
     gene            4307..5644
                     /locus_tag="PLY81_03685"
     CDS             4307..5644
                     /locus_tag="PLY81_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF29359.1"
                     /translation="MITNNRLTEAQYNQNFSDIHPMFETKDAALVAANKCLFCYDAPC
                     TKACPTSIDIPKFIKQITTENIKGSAHTIFQSNILGAGCGKVCPVEKLCEGSCVYNLM
                     EETPVQIAKLQRYSTEKAIEENWKLFERKPSNGKKVAIVGAGPAGLSCAHVLAREGVE
                     VTIYEKESKGGGLMTYGIAAYKVTPAFCEDEVNYIISIGGIDIKYNQTLGKDFSLDEL
                     QKNYDAVYLAFGIGTINKLNIPGEDLDGVEDAIHFIYNIRTNEFKNISVGDRVAVIGM
                     GNTAIDAATQSKRLGAKEVAMVYRRTEAEKTCTQHELDIARLDGCATLWLAAPKEIIG
                     DSGKVKALVCDVMQLGEPDASGRPTPIPTGETFTLEVDMIIKATGQKPYTALVEQIGL
                     ENNKGKIILQSDKASTNVKGVFAGGDAINGGKEAVDAVQAGKDGANAILNFIF"
     gene            5647..6015
                     /locus_tag="PLY81_03690"
     CDS             5647..6015
                     /locus_tag="PLY81_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF29360.1"
                     /translation="MELVVKTENKILELSFEFSLQIIQLYKILIEHKEFVLSKQLLKS
                     GTSIGANVEEAIAAQSRKDFIAKLSIASREARETRYWLRLLDKSKLVETDYSSYLNSI
                     EHIINILTKNVKTTSETNQQ"
     gene            6095..>6879
                     /gene="preA"
                     /locus_tag="PLY81_03695"
     CDS             6095..>6879
                     /gene="preA"
                     /locus_tag="PLY81_03695"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HNF29361.1"
                     /translation="MANISSNFLGIKSPNPFWLASAPPTDKKINVLRAYEAGWGGVVW
                     KTLGSQVKNVSSRYSSVDYGGHRVMGFNNIELISDRPLDINLKEITEVVKLFPDRAMI
                     VSLMADNNKKAWHELIKKCEDAGAMGFELNFGCPHGMTERGMGAAVGQDPEIAKMVVE
                     WVMEKATIPVITKLTPNVHSVVPTGKACVEAGTNALSLINTIQSVTGVDLDTLVPNPY
                     VAGKSVFGGYCGPAVKPIALKFLTTIAQDPTTSKVPISGIGGV"
BASE COUNT         2504 a          939 c         1296 g         2140 t
ORIGIN      
        1 aagaaattca tgtttcagga ttgttccctc aagcattacc ttttatttgt tttttagcat
       61 tttggttttt aaatatgctc attatttatt atggtgtgga gagtattaaa aagttattgg
      121 tttttaaagc tattttttta ccagttgcaa ctttagcatt attattttgg gcaattactc
      181 aagcaaatgg tttagacaat attcttaatc aggaatcaaa attacatggc tcagattttt
      241 ggaaatattt ctttccagca cttacaggta tgataggttt ttgggctact ttatcattaa
      301 atattacaga ttttactagg tatgcaaaaa gccaaaaagc acaaataact gggcaagcaa
      361 ttggcttacc acctagtatg actttatttg cttttgtagg cgtagtggta acattggcta
      421 ctactataat ttatggacaa acagaatggg atatagtaaa attagcaagc aaatttgaaa
      481 gtaaattagt agtaagtttt gctatgattg gaattattat ttcaacctta gcaaccaata
      541 tagctgccaa tattgtaagc cccgcaaacg attttgcaaa ccttaatcct aaaaaaataa
      601 attttaaaat tggtggttat attacaggtg ttgttggtat tttaatttta ccatggaaat
      661 taattgcaga ccctaatgga tatattttta cttggcttat tgcatattca ggtttgttgg
      721 gacccattgg aggaataatg attgcagatt atttttttat tcgcaaacaa aaattaatag
      781 tagaagattt atataaacac aacggaatgt atggttacaa aaatggcttc aacattattg
      841 cattgattgc ttttataatt ggagtaattc ctgttgttcc aggcttttta gttactgtaa
      901 agttgataga cccaaattct gttgcaacat ggctttccat cttgtatcat tatgcatggt
      961 ttgttggttt ttttgttagt ggtattttat atttaatatt aaccgcaaag aacgctaagg
     1021 attaacgcaa agaactctaa gttatttatg acagaaaatg aaatttcaaa taaagtgatt
     1081 ggtaatggga ttgaagtaca ttcagctctc ggtccaggct tattggaaag tgcatataag
     1141 gaatgtctct attataaatt gataagctca ggattcttgg ttgaaaaaga aaaagcaatg
     1201 ccattagtgt ttgaaaatgt taaattagaa tgcggttata gaatagactt attagtagaa
     1261 aaaaaactgg ttattgaaat aaaaagtgtt gaggcattaa acgatattca tttggcacaa
     1321 acacttactt atatgaaact tggtaattat aaattaggtt tgttgatgaa ctttaatgtt
     1381 ctgagattaa aagatggcat caaaagagtt gttaacggac tttaatcttt gtaaacattg
     1441 cgtaattctt tgtgcccttt gcggtttaaa aaaataactt atgagtgtat taattaaaaa
     1501 cggaagaatc attacagcag attctgatca gactgctgac atttttatta gcggcgaaac
     1561 agttcaagcc attgggaaaa atctaaatgt acaatccgat aaggaaattg atgcaacagg
     1621 attattaatt ttccctggag gcatagaccc tcatgtgcat ttagatatgc cctttatggg
     1681 tacattcagt agcgatagtt atgaaactgg tacaagggct gctttgtatg gtggaacaac
     1741 tatggtatta gattttattt tacaaaaaca aggcaatagt ttacaagatg ctttacaaga
     1801 atggaaaagc agaagtgata ataattgtgt tggtgattat agttttcaca tggctgttac
     1861 agattttaat gaggaaacaa aaaaagaaat aaaacatttt atagaggaag aaggaattac
     1921 ttctttcaaa acttttatgg catataaagg ggccttaatg atagatgaca gacagatgat
     1981 tggtttaatg gaagaagtta aaaagcatgg tggtttaatt aatgtacatg ctaccaatgg
     2041 cgatatgata gattacttaa ttgcaaaaca taaagaggaa ggaaaactat ctcccctata
     2101 tcattattta tcgcaaccag aaattacaga agcagaagca agtgaacgat ttatagatat
     2161 ggcaagttat acaggttgcc ctggttatat tgtacatctt acttgcgaag gtgctttaaa
     2221 tgcagtacgc aatgcaacca agcgtaacca aaaaatattt gtagaaactt gtattcagta
     2281 tttaattctt gatgcaagtt tatatgaaag agattttgaa ggagctaaat gggttatgag
     2341 tccaccactt cgtgaaaaga aagatcaaca aacattgtgg gcaggtataa atcagggttt
     2401 ggtgcaagta gttgctacag accattgccc atttatgtgg gagcaaaaat tattaggtaa
     2461 aaatgatttc agtaaaattc caaacgggca tcctgcaata gaaaacagaa tggaattatt
     2521 atttagcgaa ggtgttgaaa aaaataaaat tactttaaac aaatttgtag aagtaacaag
     2581 taccaacgct gcaaaaatat ttggcatgtt tcctaaaaaa ggaaccattg ctgttggtag
     2641 cgatgcagat attgttttat tcaacccaaa tgaaaaacat gtattatctg catcaacaca
     2701 tcacatgaat gtagattata gtggttatga aggatgggaa gtaacaggaa aagtaaaaac
     2761 agttttatta agaggtaaaa ttgctattga taataatgaa tgtaaagtag agaaaggatt
     2821 tggaaagttt gtgaagcgga ataaagttgc ttctatagtt tagagtttag agtttagaat
     2881 gaagagttat gagtgattta aaaaaaacag atagtatcat tcaacaaaag tcttatgctt
     2941 ttgcattagc agttattgca ttgtataaaa acttagtaac tgataaaaaa gaattcgttc
     3001 tttcaaaaca agttttaaga agtggaactt ccattggtgc taatattcag gaagctgttg
     3061 ccagtcagtc caaaagagac ttcattcaca aattgtcaat atctttaaaa gaagcaagag
     3121 aaacccagta ttggcttaac cttttaaaag atagcagtta tattccggag tctgaattta
     3181 ataacctaaa taattcttgt gacgaggtga aaaaaattct aaccagcatt attctaacaa
     3241 ctaaagaaag atatttatcc aattcataac tcataactca taactcataa ctcataactc
     3301 ataactcata actattaact cacaactcta aactcataac tcttaactca taactaaaca
     3361 atatgcccag aataattaaa tcaggattaa ttcaaatgtc tttacctaaa acagaaggtg
     3421 aaggaacaat tgaagaaatt aaagaagcaa tggtgcaaaa gcatattcct ttaattgaag
     3481 aagcaggaaa aaaaggagtg caaatacttt gctttcagga aatattcaat acgccatatt
     3541 tctgtcctgg gcaagatgcc aattggtatg caagtgctga aacagttcct ggacctacga
     3601 tagagcgaat gcaggtttat gcaaaaaaat acaacatggt tatcattgtt ccagtttatg
     3661 aaaaagaaca agctggggtg ttgtacaata ctgctgcagt aattgatgct gatggatctt
     3721 atcttggcaa gtacagaaaa aaccatattc ctcatacagg cggcttttgg gaaaaattct
     3781 ttttcaaacc tggtaatttg ggctatcctg tttttcaaac aaaatatgca aaggttgggg
     3841 tgtatatatg ttatgataga catttccctg atggtgcaag atgtttgggt ttaaatggtg
     3901 ccgaaattgt ttataatcca agtgcaacaa ctgttggtca atctcaatat ttatggaagt
     3961 tagaacaacc tgcacatgct gttgccaatg gatattttat gggttgcatt aatcgtgtag
     4021 gaactgaaaa gccttggaat cttggaaagt tttatggcac ctcttatttt gtaaatccat
     4081 taggacaaat tattgcagaa gcaagtgaag ataaagatga actactcatt gcagaatttg
     4141 atttagattt gatagaacaa ataagaagca aatggcagtt ctttagagat agaagaccag
     4201 aaacgtatgg agaattagta agattataat atcttagaaa gaggaatcta aggcgatgaa
     4261 tactcataat cattattctt aaatcataac tcataataaa aataaaatga ttacaaataa
     4321 caggctaaca gaagctcaat acaatcaaaa cttttcagac attcatccaa tgtttgaaac
     4381 aaaagatgca gctttggttg cagctaataa atgtttgttt tgctatgatg caccttgtac
     4441 aaaagcttgc cctacaagta ttgatatacc taagtttata aaacaaatca ctaccgaaaa
     4501 catcaaaggc tctgcacata ctatttttca atcaaatatt ttaggtgctg gttgtggtaa
     4561 agtttgccct gtagaaaaat tatgtgaagg ctcttgtgta tataatttaa tggaagaaac
     4621 gcctgtacaa attgcaaaat tgcaacgata ttctacagaa aaagcaatag aagaaaactg
     4681 gaaattgttt gaaagaaaac catctaatgg aaaaaaagta gctattgttg gtgctggccc
     4741 tgcaggttta agttgtgctc atgttttagc aagagaaggt gttgaagtaa ctatttatga
     4801 aaaagaaagt aaaggtggag gcttaatgac ttatggtatt gctgcttata aagtaacacc
     4861 tgctttttgt gaagatgaag taaactatat tataagcatt ggaggaattg atataaaata
     4921 caatcaaaca ttaggcaaag atttttcgct ggatgagtta caaaaaaatt atgatgcagt
     4981 ttatttggct tttggcattg gaacaattaa taaattaaac attcctggag aagatttaga
     5041 tggcgttgaa gatgcaattc attttattta caacataaga accaatgagt ttaaaaatat
     5101 ttctgtagga gatagagtag ctgtaatagg aatgggtaat acagccattg atgctgctac
     5161 tcagtcaaaa cgtttaggag ctaaagaagt agccatggtt tatagaagaa cggaagcaga
     5221 aaaaacctgt acacagcacg aattagatat tgcaagatta gatggttgtg ctacattatg
     5281 gcttgcagca ccaaaagaaa ttattggaga tagtggaaaa gtaaaagctt tggtttgcga
     5341 tgttatgcaa ttaggtgaac ctgatgcttc tggcagacca acaccaatac caactggcga
     5401 aacgtttact ttagaagtag atatgataat aaaagcaact ggacaaaaac catacacagc
     5461 tttagtagaa caaataggct tggaaaataa caaaggaaaa attattttac aatcagataa
     5521 agcatctaca aatgttaaag gtgtatttgc aggaggagat gcaataaatg gaggaaaaga
     5581 agctgtagat gctgtacagg ctggtaaaga tggagctaat gctattttaa attttatatt
     5641 ttaaatatgg aattagtagt gaaaactgaa aataagatat tagaactgtc atttgaattt
     5701 tctctacaaa taattcagct ttataaaatt ttgattgagc ataaagagtt tgttttatca
     5761 aaacaattgc taaaaagtgg tacaagtatt ggagcaaacg ttgaagaagc aattgctgca
     5821 caatcaagaa aagatttcat agcaaagctc tcaatagcat caagggaagc aagagaaact
     5881 cgttactggt taagactttt agataaaagt aagttagttg aaaccgatta ttcttcttat
     5941 ctaaattcca ttgaacatat cataaacata ctaacaaaaa atgtaaaaac tacttcagaa
     6001 acaaaccaac aataatttat tattcataat tcataattca taattaataa tttataattc
     6061 aaaatttata attcaaaatt taaaatagtt ccttatggca aatatttctt caaatttttt
     6121 aggtattaaa tcacctaatc cattttggtt agcaagtgca ccaccaaccg ataaaaaaat
     6181 aaatgtgcta agagcttatg aagcaggttg gggtggcgtt gtatggaaaa ccttaggctc
     6241 acaagtaaag aatgtttcca gtaggtattc ttctgttgat tatggtgggc atcgtgttat
     6301 gggtttcaac aatattgagt taattagcga tagaccatta gatataaatc ttaaagaaat
     6361 tacagaagtt gtaaaacttt ttccagaccg tgctatgatt gtttctttaa tggcagataa
     6421 caataaaaaa gcttggcacg aattaattaa aaagtgtgaa gatgcaggag ctatgggctt
     6481 tgaactcaac tttggttgcc ctcatggcat gacagaaaga ggcatgggtg ctgcagttgg
     6541 tcaagaccct gaaattgcta aaatggtggt agaatgggta atggaaaaag caaccattcc
     6601 tgtaattaca aaacttacac ccaatgtaca ttcagttgtg ccaacaggta aagcttgtgt
     6661 agaagcaggt accaatgctt taagtttaat caataccata caatctgtaa ctggtgtaga
     6721 tttagataca cttgtaccca atccttatgt tgcaggtaaa agtgtttttg gaggttattg
     6781 tggtcctgca gtaaaaccta tagcattaaa atttttaaca acaatagctc aagatcctac
     6841 tacctctaaa gtgccaataa gtggcatagg tggtgtaag
//
LOCUS       DAOQHP010000075         6803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5931_length_6803_cov_2.623740, whole genome shotgun sequence.
ACCESSION   DAOQHP010000075 DAOQHP010000000
VERSION     DAOQHP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_6803_cov_2.623740"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1761)
                     /locus_tag="PLY81_03700"
     CDS             complement(145..1761)
                     /locus_tag="PLY81_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF29362.1"
                     /translation="MKYVFKNEFTIAIFAAILFIPFIGHVHLFDWDEINFAESAREMI
                     ATGNYFDVQINYSKFTEKPPLFFWMQVLSMKAFGINEFAARFPNAVCGILTLVLLFRI
                     GKKIYNENLARIWVLTYLGTLVTFLYFKSGIIDPWFNLFIFLAIYHYYLLITYENSKT
                     KQAVLVGLFLGLAVLTKGPVAILIAMLCMLTYVVLNKGKFNISTKNFILILLSCLVVC
                     FAWFGVDLLKNGPAFIIEFTQRQVAIFSTSDAGHGQPFFYHWWVLLVGCFPASIFFIS
                     GQMIQNGNKEQKEFKLWMVILFWVTLLLFSIVKTKIIHYSSLCWFPLTFIAALFIYEL
                     WEGRIKKIHTLLKILFALVGVTLGLIITVLPIVLQYKNKWVHYIKDDFAKGNLQANVQ
                     WYWIDGVGGLLLLFSVVYFLCSKKIKLKTQLLYASVTVCCFFTAVLIAPKVEAISQRA
                     NIEFFESKQNEDCYIDTWGYKSYAHFFYSKKQPANFDSTKNMQWMLYGKIDKPVYIST
                     KIQNKKILDTVQNLKMLYQKNGFVFYNRLP"
     gene            1821..2786
                     /locus_tag="PLY81_03705"
     CDS             1821..2786
                     /locus_tag="PLY81_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF29363.1"
                     /translation="MHSFKELVNIFATHFNQAHFPDNPNSLYEPCNYFLTLGGKRIRP
                     IMCLMGNELFDEIKPDAFYAATAIELFHNFSLIHDDIMDAASLRRGMQTIHTKYNPNT
                     AILSGDVMLIRCYDYINKIQPIYLPKVLHLFNKTAREVCEGQQLDMDFEKLSAVSLED
                     YIKMISLKTSVLIASSLEIGAVLGGAGEGNCKKIYDFGINLGIAFQIQDDYLDAFGDP
                     EKFGKQVGGDIKQNKKTFLLLHALEVANQNQREELINLLQTDSSDKVERVTTIFKACN
                     VDAWANELKEKYVQTAFKNLEEIAVTSVRKKYLQELASFLIKRDI"
     gene            2938..4614
                     /locus_tag="PLY81_03710"
     CDS             2938..4614
                     /locus_tag="PLY81_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF29364.1"
                     /translation="MSLFRKKSLDKINIEFTESHHQQGLKRVLNVKDLTFMGIAAVVG
                     AGIFSTIGKASFDGGPGVVFLFLITAVTCGFSALCYAEFAGRVPISGSAYTYAYVSFG
                     EIIAWIIGWALILEYAIGNIVVAISWSGYFDNLLQGLGVHLPKWLLADPFTASETFKA
                     AIAEQASGKPLSAAMQEAINIYNSAPIIAGKHIFFNLPAFIIVVLITLLAYVGIKESK
                     KSANYMVIFKIAVIIFIIVMGFFYVDAKNWNPFLPNGFGGVLAGVSAVFYAYIGFDAI
                     STTAEECKNPQRDLPKGMIYSLLICTVLYILIALVLTGMVNYTELNVTDPLAFVFEKL
                     GLHKIGYIISISAVVATTSVLLVFQLGQPRIWMSMSRDGLLPKRFAKVHPKFGTPSFA
                     TIVTGLLVGIPTLIMPADLVTDLTSIGTLFAFVLVCLGVLVLPKLSKEQTKGRFRLPY
                     INGKFIIPALVILFIWFTKERIENSFLQIGSESYHEILFLIFILISVVIAVFSFIKNY
                     SLIPIIGALCCMYLMIEIPAESWIWFFVWMAIGLSIYFMYGRKRSRLNNA"
     gene            complement(4835..>6803)
                     /locus_tag="PLY81_03715"
     CDS             complement(4835..>6803)
                     /locus_tag="PLY81_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNF29365.1"
                     /translation="AWSENKGVTHFTHWFQPLTGTTAEKHDSFFTLKSDGTAIEEFDG
                     AALVQQEPDASSFPNGGLRATFEARGYTAWDPSSPAFIMEMCNGKTLCIPTIFISYTG
                     ESLDYKAPLLKALEALNKSAVDVCNYFDKNITKVSATLGWEQEYFVIDEGLANARPDI
                     MQCGRTVFGASPAKGQQLEDHYFGSIPDRIYTYMRDFEAEAYKLGIPLRTRHNEVAPG
                     QFECAPIFEEVSVAVDHNTLLMDLMERVASRHQLKVLFHEKPFDGINGSGKHNNWSMS
                     TDTGVNLLAPGKTPKTNLMFLTFFVNSIKAVHDYADLLRAAIASAGNDHRLGANEAPP
                     AIISVFVGQYLAKVLEEVETRVGDKFDEQDEAILKLDIHKSIPELLLDNTDRNRTSPF
                     AFTGNKFEFRAVGSSANCANAMTTLNTIMAETLKNFKHEVDSLIEKGEKKEIAILQTL
                     QKYITHSKKVLFEGDGYSEAWHKEAKKRGLPNVPTTPKALDAMVTDKAKKIFENNGVF
                     TLAELEARHDIELEKFIKKVQIEARVMGDLALNHIIPVAVKYQNELLNNINGLKQIGL
                     PAKNYAVQLKIVEEISNHLQQVYNNADALVEARKVANKIEDTRTKAIAYDTDVKAKYF
                     EILRYHVDKLEHLVCNNEWTLPKYRELLFVK"
BASE COUNT         2268 a         1059 c         1000 g         2476 t
ORIGIN      
        1 atctcttaaa gcagttgcaa taacttttgc tcctctatca tgtccatcta atccaacctt
       61 tgctactaaa actcttattg gtctattcat aattatttaa tataatatag tgtaaaagta
      121 attatttaaa atattcattt tgttttatgg caatctatta taaaatacaa agccattttt
      181 ttggtataac atttttagat tctgtaccgt atctaatatt tttttatttt gaatttttgt
      241 agatatgtaa actggtttat caatttttcc atacaacatc cactgcatat tttttgttga
      301 atcaaaattt gctggttgct tttttgaata aaagaaatga gcataacttt tgtaacccca
      361 agtatctata taacaatcct cgttttgttt gctttcaaaa aattcaatat ttgctctttg
      421 agatattgcc tctactttag gtgcaattaa tacagcagta aaaaaacaac atacagttac
      481 actagcatac aataactgcg tttttaattt tattttttta ctacacaaaa aatatacaac
      541 agaaaataaa agtaacaaac ctcctactcc atctatccaa taccattgaa cattggcttg
      601 caaattccct tttgcaaaat catcttttat ataatgtacc catttatttt tatattgtaa
      661 tactatgggt aaaacagtaa ttattaagcc taatgttacc cctactaaag caaataatat
      721 ttttaataga gtgtgtattt tttttattct cccctcccat aattcatata taaataatgc
      781 agcaataaat gttaatggaa accaacataa ggaagagtaa tgaataattt ttgttttaac
      841 aattgaaaac aataataagg taacccaaaa taaaataacc atccatagtt taaactcttt
      901 ttgttctttg tttccatttt gtatcatttg cccgcttata aaaaatatgg aagcaggaaa
      961 acagcctact aataaaaccc accaatgata aaaaaatggt tggccatgtc ctgcatcgct
     1021 agtactaaaa atggcaacct gcctttgtgt aaattctatg ataaatgctg ggccattttt
     1081 taataaatct actccaaacc atgcaaagca aacgactaaa caactaagta agataagaat
     1141 aaaattttta gttgaaatat taaatttacc tttgtttaat actacatagg ttagcataca
     1201 aagcattgca attaaaattg ccacagggcc tttagttaaa acagcaagcc ctaaaaaaag
     1261 tccaactaaa acagcttgtt tggttttact attttcatac gttattaaaa gataataatg
     1321 gtaaatagct aaaaaaataa ataggttaaa ccaaggatct ataataccac ttttaaaata
     1381 taaaaaggtt actaaagtgc ctaaataagt tagcacccaa atacgagcta ggttttcgtt
     1441 ataaattttt ttaccaattc taaatagtaa tactaaagtt aaaataccac aaacagcatt
     1501 tggaaatcga gcagcaaact cattaatacc aaaggctttc atacttaata cttgcatcca
     1561 aaaaaacaat ggaggctttt cggtaaactt ggaataatta atttgaacat caaagtaatt
     1621 acctgttgca atcatttctc tggcactttc tgcaaaattt atttcgtccc aatcaaataa
     1681 atgaacatgg cctataaaag gaataaaaag tattgctgca aaaattgcaa tggtaaattc
     1741 atttttaaat acatacttca tgttagtgca aaagtaagta agacagctta gttccttatt
     1801 tttgttgaaa ataaagtttc atgcattcgt ttaaagaatt ggtaaatatt tttgcaactc
     1861 attttaacca agcacatttt ccagacaatc ctaacagctt atatgaacca tgtaattact
     1921 ttttaacttt aggaggtaaa agaataagac ccattatgtg tttaatgggt aatgaattat
     1981 ttgatgaaat taaaccagat gcattttatg cagccactgc aatagaactg tttcataatt
     2041 tttctttaat acatgatgat attatggatg ctgcctcttt aagaagagga atgcaaacaa
     2101 ttcacacaaa atacaatcct aatactgcta ttttaagtgg agatgtaatg ctaataagat
     2161 gttatgatta catcaacaaa atacagccaa tttatttgcc taaagtgctt catttattta
     2221 ataaaacagc tagagaagtt tgcgaaggcc aacaattaga tatggatttt gagaaattat
     2281 ctgccgtaag tttagaagat tatataaaaa tgatttcttt aaaaacatcg gtattaatag
     2341 cctctagttt agaaattggt gctgttttag gtggtgcagg agaaggtaat tgcaaaaaga
     2401 tttatgattt tggaattaat ttaggtatag cctttcaaat acaagacgat tatttagatg
     2461 catttggaga cccagaaaaa tttggtaagc aagttggtgg cgatattaaa caaaataaga
     2521 aaacattttt gctgctgcat gctcttgaag ttgccaatca aaatcaaaga gaagaattaa
     2581 ttaatttatt acaaacagat tcttcagata aagttgaaag agttactact atttttaaag
     2641 catgtaatgt agatgcctgg gcaaatgaat taaaagaaaa atatgtacaa actgcattta
     2701 aaaatttaga agaaattgca gtaacctctg taagaaaaaa atatttgcaa gagcttgcat
     2761 cgtttttaat taaacgagat atttaataac agccattttg gtttaataag aattgtgttg
     2821 taaaaattaa actcctgtca tttaattata gcttaataac attattctaa aatttatatt
     2881 ttattggaat actacttata ttagcaatgt attaaaacca actaaaacta aagctatatg
     2941 tctttattcc gtaaaaaatc tttagataaa attaatattg agtttactga atcgcaccat
     3001 caacaaggtt taaaaagggt tttaaacgtt aaagatttaa cctttatggg tattgcagca
     3061 gttgtaggtg ctggtatttt ttctacaatt ggcaaagcat cttttgatgg aggacctggt
     3121 gttgtatttt tatttttaat aactgctgta acctgtggtt ttagtgcttt gtgttatgca
     3181 gagttcgctg gcagagttcc aatttctggc agtgcttaca cctatgctta tgtaagcttt
     3241 ggagaaataa ttgcatggat aattggttgg gctttaattt tagaatatgc tattggtaat
     3301 atagttgttg caataagttg gagtggatat tttgataatt tattacaagg tttaggagtt
     3361 catttaccca aatggttatt agccgatcct tttactgcat ctgaaacttt taaggctgcc
     3421 attgcagaac aagctagtgg aaaaccatta tctgcagcta tgcaagaagc tattaatatt
     3481 tacaatagtg ctccaataat tgcaggtaaa catattttct ttaatctgcc tgcatttatt
     3541 attgttgtac tgataacctt acttgcttat gtaggtatta aagaaagtaa aaagagtgct
     3601 aactatatgg taatttttaa aattgcagtt atcattttta ttatagtaat gggatttttt
     3661 tatgtggatg caaaaaactg gaacccattt ttacctaatg gctttggtgg tgttttagct
     3721 ggcgtatctg ctgtgtttta tgcctacatt ggctttgatg caataagtac tactgcagaa
     3781 gaatgtaaaa atccacaaag agatttacca aaaggtatga tatactcttt actaatttgt
     3841 actgttttat acatattaat tgcattggtt ttaaccggca tggttaatta tactgaatta
     3901 aatgtaaccg acccattggc ttttgttttt gaaaaattag gcttacataa aataggttat
     3961 ataatttcca taagtgcagt agttgcaact accagtgtat tattagtttt tcaattaggt
     4021 caacctagaa tttggatgag catgagcaga gatggattat tacctaaaag atttgctaaa
     4081 gtacatccta aatttggcac cccttcgttt gcaactattg ttacaggttt attagttgga
     4141 ataccaactt tgataatgcc agccgacctt gtaactgacc ttacaagcat tggtactctg
     4201 tttgcatttg tattagtttg tttaggcgtt ttagttttac ctaaactaag taaagagcaa
     4261 acaaaaggaa ggtttagatt accttatatt aatggcaaat ttattattcc agctttagta
     4321 atattgttta tttggtttac taaagaaaga atagaaaatt cttttttaca aataggaagt
     4381 gaatcttacc acgaaatttt atttttaatt tttatcctta tctctgttgt tattgcagtt
     4441 tttagtttta taaaaaatta ttctcttatc ccaattattg gtgctttatg ttgtatgtat
     4501 ttaatgatag aaattcctgc agaaagttgg atttggtttt ttgtttggat ggcaattggt
     4561 ttaagtattt attttatgta tggtagaaaa agaagcaggt taaataatgc ataaatgtga
     4621 atgtttatat tttatttagg tgttttctgt aaatattttt tttaattatc tcgtcttttg
     4681 tgcaagcatt gataaagtgc aaaagcatgt aaggaatatt aactttgtgc attaacaatt
     4741 cacatcaaaa agttttaatt gtacattatt gtaaataaaa agcaccgtta tttattataa
     4801 taacgatgct taatttattg gttaaaataa ctgtttactt cacaaataac aattctctat
     4861 atttaggtaa agtccattca ttattgcaaa ctaaatgttc taatttatct acatgataac
     4921 gtagtatttc aaagtattta gccttcacat ctgtatcata agcaattgct tttgttctcg
     4981 tatcttcaat tttatttgct acttttctag cttcaaccaa agcatctgca ttattgtaaa
     5041 cttgttgtaa gtggttgcta atttcttcta caattttaag ctgtacagca tagttttttg
     5101 ctggtaatcc aatttgcttt aagccattga tattattcag taattcattt tgatatttta
     5161 ctgcaacggg tataatatgg ttcagtgcta aatctcccat tacccttgct tctatttgta
     5221 cttttttaat aaatttttca agctcaatat catgcctagc ttctaattca gctaaagtaa
     5281 atactccatt attttcaaat atttttttag ctttatctgt aaccattgca tctaatgctt
     5341 ttggtgttgt tggcacattt ggtaagcctc tctttttagc ttctttgtgc caagcttcgc
     5401 tatacccatc tccttcaaat aatacttttt tactgtgtgt aatatatttt tgcaatgttt
     5461 gtaaaattgc aatttctttc ttttctcctt tctctattaa gctatccact tcgtgtttaa
     5521 aattcttcaa agtttctgcc attatagtgt ttaaagtggt cattgcatta gcacaatttg
     5581 cactagagcc aactgctcta aactcaaatt tatttcctgt aaaagcaaaa ggggatgttc
     5641 tatttctatc ggtattatct aataataatt caggaatact tttatgtata tctaatttca
     5701 aaatagcttc atcttgttca tcaaacttat ctcctaccct tgtttctact tcttctaaaa
     5761 cttttgctaa atattgcccc acaaataccg aaataattgc tggtggtgct tcattagcac
     5821 ccaacctatg gtcgttacct gcacttgcaa tggcagctct taataaatcg gcataatcat
     5881 gcactgcttt aatgctattt acaaaaaatg taagaaacat taaatttgtt ttaggagttt
     5941 tgccaggtgc taataagttt acacctgtat ctgtactcat actccagtta ttatgttttc
     6001 cactaccatt aataccatca aatggttttt catgaaaaag aacttttaat tggtgcctgc
     6061 tagcaacacg ttccattaaa tccataagca gtgtattgtg atctacagca acactcactt
     6121 cttcaaatat aggagcacac tcaaactggc ctggagccac ttcattatgc cttgttctta
     6181 atggaatacc taatttataa gcttcagcct caaagtctct catataggta taaattctat
     6241 cgggaataga gccaaaataa tgatcttcta attgttgtcc ttttgcaggt gatgcaccaa
     6301 aaactgttct tccgcattgc ataatgtctg gtctggcatt tgccaatcct tcatctatta
     6361 caaaatattc ttgctcccag ccaagtgtag cagaaacctt cgtaatattt ttatcaaaat
     6421 aattacaaac atctactgca cttttattaa gtgcttctaa tgcttttaat aaaggtgctt
     6481 tataatccaa agattcgcct gtataggaaa taaaaattgt aggaatacac aaggttttac
     6541 cattacacat ttccataata aatgcaggag aagaaggatc ccatgcagta taacctcttg
     6601 cctcaaaagt ggctcttaat ccaccatttg gaaaggagga agcatctggc tcttgttgca
     6661 ccaatgcagc accatcaaat tcttcaatag ctgtaccatc gctttttaag gtaaaaaatg
     6721 aatcatgttt ttctgcagtt gtacctgtta atggttgaaa ccaatgcgta aaatgggtta
     6781 ctcctttatt ttcagaccat gct
//
LOCUS       DAOQHP010000076         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_5956_length_6786_cov_3.945476, whole genome shotgun sequence.
ACCESSION   DAOQHP010000076 DAOQHP010000000
VERSION     DAOQHP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5956_length_6786_cov_3.945476"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(790..3435)
                     /locus_tag="PLY81_03720"
     CDS             complement(790..3435)
                     /locus_tag="PLY81_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29366.1"
                     /translation="MKLFLSTLIIITLQINSFAQMPGGGRQRQGAQANNGHFYGKFVD
                     SKTNKPIEGVAVQLFANVFDTVSKKKVETNLRTIIAANNGDFSFEGLNTMQTYKIKAS
                     VIGYKPFEKSLKFNTKANADGDIASMVDIDLGNIKLDQDPSDLGNVTVVATKQMFEMG
                     IDRKVFNVDKNIVSTGQTATEVMKSIPSVSVDIDGNVQLRNATPEIFIDGRPTTLTLD
                     QIPADIIDRIEIITNPSAKYDASGGNAGIINIILKKNKRTGYNGGISTGIDSRGKINL
                     GGDFNYRENKINFFGSGRFNQRKSISTSYANTNFTGNNPFNVKSNSENDNNGYFAFLR
                     GGLDYFVNNRNTISVSANYNKGQFNSDAIQNVDTTTTSLQATSNRITDSKFTFENFGT
                     QLSYKHNFTKSGHNLSADINYNSSKNSNNSNINSLIYNSNGTPKYPLNQQQGIGDGTN
                     KFFTLQTDYENKLTENSKLELGVRAAIRNYTTNNLQYINYNTNNVGFVLSTAASNRYK
                     FNDQVFAAYSTYSFKYNKFSFQLGLRAESSNYTGNLLTLAGKDSSSFKVNYNLSLFPS
                     AFITYKLSDKEDLQLNYSRRVNRPGFFQLLPTYDFSDPQNPSVGNPALNPEFTNSYEF
                     SYNNNYKRGANFLATAYFKHSNNLVTRYIYKDINRNIQSGQTTNDSLFYTSYINASTS
                     YSYGLELTNKMNITKWWDLTLNLNLYNSVINANIPGQTIDNSIVSWFGKINNNFKIMK
                     GLSVQLSSDFRSKTIIPQGGGRGGRGGGGGGMWGGSPTLAQGYTLPRYFDVDLAIRKD
                     WNWKNGQSGSLTLSMGNIFNVPVKTYTEAIYFTQDTDRLRDRQVLRLNFSYRFGKFDV
                     SLFKRKNSKADQGEGGGDMGGGIGM"
     gene            complement(3588..4193)
                     /locus_tag="PLY81_03725"
     CDS             complement(3588..4193)
                     /locus_tag="PLY81_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNF29367.1"
                     /translation="MNYYWLVLIPIISALIGWFTNYIAIKMLFYPKNPTYIFGINFQG
                     VFPKKQKQFAEKLGKLVSNELLSFKEIEDKIANPQNIQKIMPLVEEHIDGFLKVKLAE
                     QMPVISMFISYKTIDQLKTVFMNELEVLFPSIMNNYMGKLKAELDLEKIVTEKVNNFS
                     SDKLEEILNSIMKKELKFVEILGGVLGFIIGIFQVLITLLV"
     gene            complement(4254..4327)
                     /locus_tag="PLY81_03730"
     tRNA            complement(4254..4327)
                     /locus_tag="PLY81_03730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4291..4293),aa:His,seq:gtg)
     gene            4487..6622
                     /locus_tag="PLY81_03735"
     CDS             4487..6622
                     /locus_tag="PLY81_03735"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNF29368.1"
                     /translation="MKSRKGIIVLITLVFGILCFAFANNISEGGEKDSLEQKKQLLSS
                     IGLMIEKQHYHPKKIDDNFSKQVLKEFLEGLDNEKNIFILSDIEVFKKYENYIDDEMH
                     GGDIKVVPAITTVYERRILELEKVYQNILSSAFNFDSSDVYITDYKTLNYAKNEEERN
                     DRWRKKLKYLTLERYVDLLTNRDKLKQTDTAYKNNEQLEEQARKMVLSIMNRTFEKIK
                     ISFTQDKLFNTYVNVITNYIDPHTDYFPPIEKRAFDENMSGRFYGIGAQLQEQEGVIK
                     IASVMAGYPAWRSGEIEQNDVIVKVAQGNGEPIDITGYDVTDAVKLIRGNKGTEVRLT
                     IKKQNGTIKVVSLIRDEIIQDESYARSAVINDNGKKVGYIYLPDFYADFERPDGARCS
                     EDVAKEIMKLKVENVEGIVFDLRSNGGGSLYEVVQMVGLFIKTGPVVQVKDKAGKISV
                     LKDDNPLVLYDGPLAVMVNGFSASASEIFAAAIQDYKRGIIIGTNTSTYGKGTVQRNI
                     PLGKVLDYQTGRTEFGAIKLTFQKFYRINGGSTQLKGVVPDIVMPDTYEYLEFREKDN
                     SNSLPWDEIPKADYAVDSNSNELNFIISSENKMISENNNLNIIHNNALWLANNTKKPI
                     SLNFDNYKIFQKQIKSTVAQTTTLSKLSKEMDVTVTSADKSKYFSNVDKAKGDRYQAW
                     LKNIKNDMYISTTADMIVNMANKKIVYNY"
BASE COUNT         2370 a          935 c         1006 g         2475 t
ORIGIN      
        1 atttattgag aggctttttt attgtattta ccctacttaa attaattttc aatttttagc
       61 aatattttta ttttttatta gtattattct attttataaa tgcattcggt ttgtgtaatg
      121 attgcaacaa ggttttatat tttgtaatac aattatctaa attttattaa ttcattttgt
      181 aactacaact aaatatttag tattttatat tttttatttt ggattgttac tgtaaatatt
      241 tttgtttata tgtcacttag ggcaaataaa cataaatatt atatagggat atttaatgct
      301 attaattttt gctctttaat actttgttag tacataatac tacatataaa ttttcagatg
      361 atgtaatgtg catcatacgt gaaatgagat agttagtaaa tatgaaaatt cataaataca
      421 ttctgtagat tgtttttttt tgctatgtta tttcatttta atactttgaa actttagttt
      481 ttagaactca aatccttcgt ctgttctaat agtgttttta tataggatgt aggtagagtt
      541 ttgttctatt atattcaaat ttttaagttt tactcttgta gaaaacaaaa agaaagaaat
      601 attcattaaa ctaaagaaat tgttcaaaat atttttagaa ttgcttttat agttttttaa
      661 ttctttgtta ttgattacgg cttctctatc ttaatattta acatactgtc ttaaattttt
      721 aaagctaccc ataatgagta gctttaaaat ataataaaga aatatacatt tgtaattatc
      781 aactgaaaac tacattccaa ttccacctcc catatctcca ccaccttctc cctgatcggc
      841 tttagaattt tttcttttaa atagagatac atcaaatttt ccaaaacggt aagaaaaatt
      901 taatctaaga acttgtctat ctcttaacct gtctgtatct tgagtaaaat aaattgcctc
      961 ggtataagtt ttaacaggta cattaaaaat attacccata cttaaagtta aagaaccact
     1021 ttgaccattt ttccaattcc aatcttttct tatagcaagg tccacatcaa aatatcttgg
     1081 taaagtgtaa ccttgagcca gagtaggaga tcctccccac ataccaccac cacctcctcc
     1141 tcttcctcct ctaccaccac cttggggtat aatagtttta gaacgaaaat cgctagaaag
     1201 ttgtactgaa aggcccttca ttattttaaa attattattt atttttccaa accaacttac
     1261 aatagagttg tcaattgttt gccctggaat atttgcattg ataacagaat tgtaaagatt
     1321 tagatttagt gttaaatccc accattttgt gatattcatt ttattagtaa gctctaatcc
     1381 ataagaatag cttgtactag catttatata actggtataa aataagctat cgttagttgt
     1441 ttgtccactt tgtatatttc tattgatgtc tttataaata tatcttgtaa ctaaattgtt
     1501 gctgtgttta aaataagctg ttgctaaaaa gttggcacct cttttataat tattattata
     1561 agagaattcg tatgaatttg taaattctgg atttaaggct gggttaccta cacttggatt
     1621 ttgagggtcg gagaaatcgt aggttggtaa taattgaaaa aagcctggtc tatttactct
     1681 tctagaataa ttcaattgta ggtcttcttt atcgcttaat ttataggtaa taaaagcact
     1741 tggaaataga ctaagattgt agtttacttt aaaagaggag gaatcttttc ctgctaatgt
     1801 taaaaggttt ccggtatagt ttgaactttc ggcccttaag cctaattgga aactaaattt
     1861 attatattta aagctatatg ttgaataggc tgcaaaaact tgatcgttaa atttataacg
     1921 gttacttgct gctgtactta atacaaaccc tacattgtta gtattgtaat taatatattg
     1981 taaattgttg gtagtataat ttcttatagc tgctctaaca cctaattcta gtttagagtt
     2041 ttcggttaac ttattttcat aatctgtttg aagagtaaaa aacttgttgg ttccatcacc
     2101 aatgccttgt tgttgattta atggatattt tggcgttccg ttagaattgt aaattaaaga
     2161 attgatgttt gagttgttgc tatttttact tgagttgtaa ttgatatctg ccgataggtt
     2221 atgcccactt ttagtaaagt tgtgtttgta acttaattga gttccaaaat tttcaaatgt
     2281 aaattttgaa tcagtaattc tatttgaagt tgcttgtaag cttgtagtag ttgtatctac
     2341 attttgaatt gcatctgaat taaactgccc tttattatag tttgctgaaa cagaaattgt
     2401 atttctattg ttcacaaaat aatctaagcc acctcttaag aatgcaaaat aaccattgtt
     2461 gtcattttcg ctgtttgatt tcacattaaa aggattattg cctgtaaagt ttgtatttgc
     2521 ataagaagtt gaaatagatt ttctctgatt aaatctaccg cttccaaaaa agttaatttt
     2581 attttcacga tagttaaaat ctccgcctaa atttatttta cctctagagt caatacctgt
     2641 actaattcct ccattatagc ccgttctttt atttttctta agaataatat ttatgatacc
     2701 tgcattacct ccacttgcat catattttgc agaagggtta gttataattt ctattctatc
     2761 aataatatct gcaggaattt gatctaatgt taatgtagtt ggtcttccat ctataaatat
     2821 ttctggagtt gcatttctta attgtacatt tccatcaata tcaaccgaaa cagaagggat
     2881 agatttcatt acctctgttg ctgtttgacc tgtactaaca atatttttat ctacattaaa
     2941 aacttttcta tcaataccca tttcaaacat ttgtttagtt gcaacaactg ttacattacc
     3001 taggtcggat gggtcttgat ctaattttat atttcctaaa tcaatatcca ccatactagc
     3061 tatatcgcca tctgcatttg cttttgtatt aaattttaag cttttttcaa aaggcttata
     3121 accaattaca cttgctttaa ttttataggt ttgcattgta tttaaacctt caaaactaaa
     3181 atcgccatta ttggctgcta taattgttct taaattagtt tctactttct tttttgatac
     3241 agtatcaaaa acgttagcaa ataattgaac agctacacct tctattggtt tattagtttt
     3301 agagtcaaca aatttaccat aaaaatgccc gttgtttgct tgtgcacctt gcctttgcct
     3361 accaccacct ggcatttgag caaaactgtt aatttgtaag gtaataatga ttaaagttga
     3421 aaggaataat ttcataatac ttattatata atagttgaaa ccacaaatat tctacgaaac
     3481 ttttagtttt tagttatata aagtataagt ggtaatttat taggctaaga ggagtaaaat
     3541 tagtttatag actatttaat aacctgaaaa gtttagttaa tgaaatgtta tacaagtaaa
     3601 gtaattagaa cttgaaaaat acctataata aagcctaaaa ctccacctaa aatttctaca
     3661 aatttcagtt cttttttcat tatgctattt aaaatttctt ctaatttatc ggatgaaaag
     3721 ttattcactt tttcagttac aattttctcc aaatcaagct ctgcctttag tttgcccata
     3781 taattgttca tgatggatgg aaatagtact tccaattcgt tcataaacac tgtttttaat
     3841 tggtcaatgg ttttgtaact aataaacata ctaataactg gcatttgctc ggctaatttt
     3901 actttcaaaa aaccatcaat atgctcttcc accaaaggca taattttttg tatattttga
     3961 ggattagcaa ttttatcttc aatttcttta aaagaaagta gttcgttgct tactaatttt
     4021 cctaactttt ccgcaaattg cttttgtttt tttggaaata cgccctgaaa gttaatacca
     4081 aatatataag ttggattttt agggtaaaac aacattttta ttgctatata gttagtaaac
     4141 cacccaatta atgcagaaat aattggtatt aatacaagcc aatagtaatt cataatgaaa
     4201 tatttaaaaa agaagccttg taatattttt gtttacaagg cttaaaattc aagggagggt
     4261 aatgggtctc gaacccacga cctacagtgc cacaaactgt cactctaacc aactgagcta
     4321 taccctccat ttataaagga ttgcaaaaat agcagaaaaa atgattctgt aaaaatgatt
     4381 ttttatcaat atttattaaa acctatgtta atagtataaa tgggaatagg atacttttaa
     4441 aaagtagtac atttgaaaaa taattatgca aaaatttatt caatttatga agagtagaaa
     4501 aggtattata gtgttaataa cattagtgtt tggtatttta tgttttgctt ttgcaaataa
     4561 tatatcagaa ggtggcgaaa aggattcatt agagcaaaaa aaacaattac tttcctccat
     4621 aggcttaatg atagagaaac aacactatca ccccaaaaaa attgatgata acttttctaa
     4681 acaagtatta aaagagtttt tggaagggct ggataatgaa aaaaatattt ttattctatc
     4741 agatatagaa gtttttaaaa agtatgaaaa ttatattgac gatgaaatgc atggtggcga
     4801 tattaaggtt gtgccagcta taactacagt ttatgaaaga agaattttag aacttgaaaa
     4861 agtgtatcaa aatattttat catctgcctt taattttgat tcaagtgatg tttatattac
     4921 tgattataaa acattaaact atgctaaaaa cgaagaggag agaaatgaca ggtggagaaa
     4981 gaaattaaaa tatttaaccc ttgaaaggta tgtagattta ttgaccaaca gagataaact
     5041 aaaacaaact gatactgctt ataaaaataa tgagcaatta gaagaacaag caagaaaaat
     5101 ggttttaagc attatgaata gaaccttcga aaaaattaaa atttctttta ctcaagataa
     5161 attatttaat acttatgtta atgttattac caattatata gacccacata cagattactt
     5221 tccaccaatt gaaaaacgtg catttgatga aaatatgagt ggaagatttt atggtattgg
     5281 agcacagcta caagagcaag aaggcgttat aaaaattgca agtgtaatgg ctggctatcc
     5341 tgcatggcgt agtggagaaa ttgagcaaaa tgatgtaatt gtaaaagtag cccaaggtaa
     5401 tggagagcca atagatatta ctggttatga tgtaacagat gcagtaaaat tgataagagg
     5461 aaataaagga acagaagtaa gattaaccat aaaaaaacaa aatggtacaa ttaaagtagt
     5521 ttctttaatt agagatgaaa ttatacaaga tgaatcttat gctcgtagtg ctgttattaa
     5581 tgataatggc aaaaaagttg ggtatattta tttgccagat ttttatgccg actttgaaag
     5641 gccagatgga gcaagatgct ctgaagatgt agcaaaagaa attatgaaac taaaggtaga
     5701 aaatgtggaa ggaattgtat ttgaccttag aagtaatgga ggaggctcct tatacgaagt
     5761 ggtacaaatg gttggtttat ttattaaaac cgggcctgtt gtacaagtta aagataaagc
     5821 aggtaaaatt tctgttttaa aagatgacaa cccattagta ttatacgatg gacctttagc
     5881 agttatggtg aatggtttca gtgcttcggc ttcagaaatt tttgctgctg ctattcaaga
     5941 ttacaaaaga ggaataataa ttggcactaa tacatcaact tacggtaaag gaacagtgca
     6001 aagaaatata cctttaggta aggtacttga ttaccaaaca ggcagaaccg aatttggagc
     6061 cataaaatta accttccaaa agttttatag aattaatggc ggctccacac aattaaaagg
     6121 tgttgtacca gatattgtga tgccagatac ttacgagtat ttagaattta gagaaaaaga
     6181 caatagtaac tcattgccat gggatgaaat tccaaaagca gattatgcag tagatagtaa
     6241 ttccaatgaa ttgaatttta ttattagttc agaaaataaa atgattagtg aaaacaataa
     6301 cttaaatatt attcataata atgcactttg gcttgccaat aatactaaaa aacctatttc
     6361 attaaacttt gataattata aaatatttca aaagcaaatt aaaagcacag tagcacaaac
     6421 aacaacatta tctaaattat ctaaagaaat ggatgtaact gtaactagtg cagataaatc
     6481 aaaatatttt tctaatgttg ataaggcaaa aggagatcgt tatcaggctt ggttaaaaaa
     6541 tataaaaaat gatatgtata tttcaacaac agcagatatg attgtaaata tggcaaacaa
     6601 aaaaattgta tataactact aatttatttt tttaaatctt cttttaagca actctttcag
     6661 ggttgctttt tatttatatc cacattgtgt ggatttttac tctacttcat ctgagttgct
     6721 aaaagttaac tttgaaatgt tactttaacc cattgattta tatgccaaaa tccattacaa
     6781 gtagtt
//
LOCUS       DAOQHP010000077         6707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6049_length_6707_cov_3.625225, whole genome shotgun sequence.
ACCESSION   DAOQHP010000077 DAOQHP010000000
VERSION     DAOQHP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6049_length_6707_cov_3.625225"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1104)
                     /gene="rpsB"
                     /locus_tag="PLY81_03740"
     CDS             complement(208..1104)
                     /gene="rpsB"
                     /locus_tag="PLY81_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNF29369.1"
                     /translation="MENNTSLQQQLLEAGVHFGHLKKKWNPKMLPYIFAEKKGIHIID
                     LNKTVVHLQETAAALKQIAKSGKKIMFVATKKQAKEIVSECAKRVNMPYATERWLGGM
                     LTNFNTVRKSVKKMQSIDKMLNDGSAESLTKKERLTLSRDKEKMEKVLGGIAQLGRLP
                     SALFIVDIGVEHIALAEAKRLGISTFGVVDTNCDPNKVDFAIPANDDATKSIAIITNY
                     ITAAIAEGLAERMANKEEEEANQNDDNNENEAKAKRLEQEAQAEGGRGRGRGGRTTGD
                     GATNANNAPKRRVPNTRRPAGK"
     gene            complement(1221..1610)
                     /gene="rpsI"
                     /locus_tag="PLY81_03745"
     CDS             complement(1221..1610)
                     /gene="rpsI"
                     /locus_tag="PLY81_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNF29370.1"
                     /translation="MEKQKNAVGRRKEAVTRVFISKGDGKITVNDKDYKTYFPLAYLQ
                     NQVEAPLKTVEATGKFDIVVNAQGGGLKGQAEAIKLGISRALIEVNSELRPALKAAGY
                     LKRDPRSVERKKFGHKKARKSYQFSKR"
     gene            complement(1655..2098)
                     /gene="rplM"
                     /locus_tag="PLY81_03750"
     CDS             complement(1655..2098)
                     /gene="rplM"
                     /locus_tag="PLY81_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNF29371.1"
                     /translation="MSKLHFTTKHANAATVTHNWYVVDGTNQTVGRMCSKIAAILRGK
                     NKPYYTPHVDCGDYIIVTNCDKVKFTGNKLGEKIYDTFSGYPGGRKEEVAKDLIKRRP
                     EVIIERAVKGMLPKNRLGRKMYKKLFVYTGSSHPHTAQQPKELKF"
     gene            complement(2675..3238)
                     /locus_tag="PLY81_03755"
     CDS             complement(2675..3238)
                     /locus_tag="PLY81_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNF29372.1"
                     /translation="MIHSVRFIAVLDTNVVYPVIIRDILFWFAYYDLYTPKWSEHIFD
                     EWKRVMVEKEVSEEEANKRISKANSAFPDALVQNYKGLIDHLELPDKDDRHVLAAAIK
                     TNANLIVTNNIKDFPEEYLQSFSLSAKTADDFLTDIIDLNQEQAVAAFKEMVLNKKNP
                     KQDEFEVLNLLRNAGLKDTANYLHALL"
     gene            complement(3228..3683)
                     /locus_tag="PLY81_03760"
     CDS             complement(3228..3683)
                     /locus_tag="PLY81_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF29373.1"
                     /translation="MKVLEQIRKPSKLDQKIASESYDALASVIEQLNSEQPEIEIEET
                     SEKIRIPLSALKLLGDILKAMGQGKLISLVPIATEVTTQAAAEILGCSRPHLVKLLED
                     GKIAFTKVGKHRRIKFDDIMRYRKQMKEQQKQNIIDIMNLDEETGLYDS"
     gene            4032..4781
                     /locus_tag="PLY81_03765"
     CDS             4032..4781
                     /locus_tag="PLY81_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF29374.1"
                     /translation="MLNGKKVIVVLPAYNAEKTLEQTYNEIPLNVVDEVILVNDASRD
                     NTVGEAERLGIKHIVTHTNNRGYGGNQKSCYNKALELGADIVIMVHPDYQYTPKLIIP
                     MCSIIANEVYPVVFASRILGKGALKGGMPMYKFIANRILTLTQNILLNQKLSEYHTGY
                     RAFSRVVLEKVNYNANSDDFVFDNQMIAQIFDAGFEIGEVTCPTKYFEDASSINLKRS
                     ITYGLGVLKVSFGYFFKKLGLNNSVIYNHKK"
     gene            4827..5498
                     /gene="ung"
                     /locus_tag="PLY81_03770"
     CDS             4827..5498
                     /gene="ung"
                     /locus_tag="PLY81_03770"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNF29375.1"
                     /translation="MDVQIEASWKAVLQQEFTKNYFQQIVAHLKTEKATKTIIYPSGS
                     LIFNAFKQTPFNQVKVVILGQDPYHGIGQAMGLSFSVPNGVSNPPSLQNIFKELQTDI
                     GMPIPSSGNLTAWAKQGVLLLNAVLTVRANEAASHSKIGWTNFTDAVIQKLSDEKIHL
                     VFLLWGKFAQEKQILIDETKHSVLKAAHPSPLSAYNGFFGCKHFSRTNEILVKNGVEP
                     INWSF"
     gene            5512..6324
                     /locus_tag="PLY81_03775"
     CDS             5512..6324
                     /locus_tag="PLY81_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF29376.1"
                     /translation="MKQLLEFFKNVFARIWALWGLVTFVVTFFIFFIPSMCTHLIADV
                     NKSQNVFIYIAKIWMNVWLFLVGCRVKVRGKENVEKNKTYIFTCNHNTLLDVPITCPY
                     IPGGNKTIAKKSFVKIPIFGWYYAKGGILIDRKSDASRRMGFEQMKKTLQQKIHVCIY
                     PEGTRNRTGKPLKSFYDGAFKLAELAQTPIVPTMLYNTLKAMPNNKFFYLLPHSLEIH
                     FLPAISPQNLTMKELKDKVFDIMYKEFELNNKKKTVSIFYLLKQLTSIFIIT"
     gene            complement(6317..>6707)
                     /locus_tag="PLY81_03780"
     CDS             complement(6317..>6707)
                     /locus_tag="PLY81_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNF29377.1"
                     /translation="VALSKQLGKIPCVVNDYPGFIANKILMPMINEAIISLYEGIAGV
                     EEIDTIMKLGMAHPMGPLQLADFIGLDVCLSILNVLYNGYGNTKYAPCPLLVNMVTAG
                     KLGVKSGEGFYTYTAGSKDLVVSSRFK"
BASE COUNT         2178 a         1088 c         1012 g         2429 t
ORIGIN      
        1 atatctgcag cagttatagc tgttgttgta ctcatggtat tttaagttta atatttttta
       61 taaggttatg tttaagatat ttattattga aaatatgttt agtataataa ataatatttt
      121 gaatgaaaaa ttaataaata gataaaaagc aaattggctg actatacaaa taatcaacca
      181 attaattgct attatttaac tgataaatta tttacctgct ggtctgcgag tattaggtac
      241 tctacgttta ggagcattat tagcattagt agcaccatca ccagttgttc taccacctct
      301 gccacgtcca cgacctcctt cagcttgtgc ttcttgttct agtctttttg ccttagcttc
      361 attttcgtta ttgtcgtcgt tttggtttgc ttcttcttct tctttatttg ccatacgttc
      421 agccaagcct tctgcaattg ctgcagtaat ataattggta ataatggcaa tagattttgt
      481 agcatcatca tttgcaggaa ttgcaaaatc tactttatta gggtcgcaat tagtatccac
      541 aactccaaaa gtagaaatac ctaaacgctt agcttctgct aatgcaatat gctctacacc
      601 aatatcaaca ataaataaag ctgatggtaa acgacctaat tgagcaatac cacccaatac
      661 tttttccatt ttttctttat cacggctaag agttaaacgt tcttttttag ttaggctttc
      721 tgcactacca tcgtttaaca ttttatcaat gctctgcatt tttttaacac ttttgcgtac
      781 agtattaaaa ttggtaagca taccacctaa ccaacgttct gttgcataag gcatatttac
      841 acgtttagca cattcgctaa ctatttcttt tgcttgtttt tttgttgcaa caaacataat
      901 ttttttaccg ctttttgcaa tttgctttaa agcagcagct gtttcttgta agtgaacaac
      961 agttttattt aaatcaataa tatgaatacc ttttttttca gcaaaaatgt aaggtaacat
     1021 tttaggattc cacttctttt ttaaatgtcc aaagtgtaca cctgcttcta atagctgttg
     1081 ttgaagtgaa gtgttatttt ccataattta tgtggctttt atactctatt aaccagagaa
     1141 aaagctttat ttttaaagtt gaataaaatt tattaagaac tttgaattta ctcaatacta
     1201 tatagtgtaa ttgaataaaa ttaacgtttg ctgaactgat agctttttct ggctttttta
     1261 tgaccaaatt tcttacgttc aacactacgt ggatctcttt ttaaataacc agcagctttt
     1321 aaagcaggtc ttaattcaga atttacttct attaacgccc tagatattcc taactttatt
     1381 gcttctgcct gtccttttaa accaccacct tgtgcattaa caacaatatc aaacttacct
     1441 gttgcttcta ccgtttttaa aggagcttct acctgatttt gtaagtaagc taaaggaaaa
     1501 taagttttat aatctttatc atttacagta atttttccat cgcctttgct gatgaataca
     1561 cgtgttacag cttctttacg acgaccaact gcgttttttt gtttttccat tttataatag
     1621 attggaatgt tttaaatttt gttttgtaat taagttagaa ttttaactct tttggttgtt
     1681 gtgctgtatg aggatgagac gaaccagtat atacaaataa ttttttgtac attttacgtc
     1741 ctaatctgtt tttaggcaac atacctttta ctgctctttc aataattact tccggacggc
     1801 gtttaattaa atctttagca acttcttctt tacgtccacc aggataacca gaaaaagtgt
     1861 cgtaaatttt ttctcctaat ttattacctg taaattttac tttatcacag ttagtaacaa
     1921 tgatatagtc tccacaatct acgtgtggtg tgtagtaagg tttgttttta cctcttagta
     1981 ttgctgcaat tttgctgcac atacgtccta cggtttgatt agtaccgtcc acaacatacc
     2041 aattatgtgt tacggtagcc gcatttgcat gctttgtagt gaaatgtaat ttgctcattt
     2101 tattttttgt tttaattaaa tcaatgctat cccattgatt ttaaatagcc ctttttaaaa
     2161 ggattgcaaa attaggtgtg ttatctttta ttttcaaaat tttgtagaaa tattttttac
     2221 cgcaattttg taactaattg atttttagta aaaaaattga caaaatattt ttatacatag
     2281 ctgaaatgct tgaaaatatt gatgttttta ctaaacttat tcttaaaaag aagctattat
     2341 agtgcattac acaatttatt ctacaaaaaa tatctttatt aaatccgcat gaaacaacaa
     2401 actaattttc atactttata gaatatatga tacaattaaa tatttgggta atggtaaagc
     2461 attggcgatg ttgcgttatt ctgtgttacg tctgcaaggt gccgctgctg aataaaaata
     2521 caaaagctca ttgtttattc tgttgctcgg cgttattcgt cagacgaaac tgaagctgat
     2581 tagcgatata cagccgattg ttcttctgtc gccccgccat atacaccaat gcacctgtta
     2641 gcggtttgtg ccttacgatt tcagtttgaa atatttacaa cagtgcatgc aaataatttg
     2701 cggtgtcttt aagtcctgca tttcttaaga ggtttagcac ttcaaattca tcctgtttgg
     2761 gatttttctt attcaaaacc atttccttga aagcagcgac agcttgttct tgattgaggt
     2821 ctataatatc ggttagaaag tcatcagcgg tttttgcact tagactaaaa gattgcaaat
     2881 actcttctgg aaagtcttta atattgtttg ttacaataag attagcattt gtctttattg
     2941 ccgcagccaa gacgtgtcgg tcatctttat ctggcaattc caaatggtct attaagcctt
     3001 tgtaattttg aactaaagcg tctgggaaag ctgaatttgc tttggatatt cttttatttg
     3061 cctcctcctc tgaaacttct ttttccacca ttactctttt ccactcatca aaaatatgtt
     3121 cgctccattt tggggtataa aggtcataat atgcaaacca aaataaaata tctctgataa
     3181 ttacaggata gacaacattt gtatctaaga cagctataaa gcgaacacta tgaatcatac
     3241 aagccagttt cttcgtctaa attcattata tcaataatgt tttgcttctg ttgctctttc
     3301 atttgttttc tatacctcat aatatcatca aattttattc ttctatgctt tccaactttg
     3361 gtaaatgcaa tctttccatc ttcaagtagc tttaccaaat gaggccttga acatccaagg
     3421 atttcagccg cagcttgagt agttacttca gtagcaatag gcacaagaga aattaattta
     3481 ccttgtccca tagcctttaa aatatctcct aatagtttca aggcacttaa tggaattctg
     3541 attttttcgc ttgtttcttc tatttcaatt tcaggttgct cagaattaag ttgctctatc
     3601 actgaagcta aagcgtcgta agattcgctt gcaatctttt gatcaagctt tgaaggtttt
     3661 cttatttgtt ctaatacttt catgagtata aatttttgag ttctgtcgca aattaaacga
     3721 aataaacgaa atagtcaaaa taaaagaaaa taaaattttg cagtttaaca atctatcaag
     3781 caagtaaaaa ttatcaatat tgattatcag tgagttagta aaagtaaagg tttgggatag
     3841 accagcagtt tttggcagtt tctcaattaa gtaaactctg tccagtggca taaccgctaa
     3901 catactattt aagctctctt aacaatgaaa tgtaatgttt attacatttt tttcttcaat
     3961 aacaatactc agttttcatt atcggttact tattaactac tatttttaca aactttacaa
     4021 aaagaaatct tatgctaaat ggtaaaaaag taatagtggt tttacctgct tataatgccg
     4081 aaaaaacatt agagcaaacc tataatgaaa taccattgaa tgtggttgat gaagtaattt
     4141 tagtaaatga tgccagccga gataatacag ttggcgaagc agaaagatta gggataaaac
     4201 atatagtaac ccataccaat aacagagggt atggaggtaa ccaaaaatct tgttacaaca
     4261 aggcattaga acttggtgca gatattgtaa taatggttca cccagactat caatatacgc
     4321 ctaaactaat aatacccatg tgtagtatta ttgccaacga agtttatcca gttgtttttg
     4381 cttctagaat tttaggaaaa ggagcattaa aaggcggaat gccaatgtat aaatttattg
     4441 caaacagaat tttaactcta acgcaaaata ttttattaaa ccaaaaatta agtgaatacc
     4501 acacagggta cagagctttt agcagagttg ttttagaaaa agtaaactat aatgctaata
     4561 gcgatgattt tgtttttgat aaccaaatga ttgctcaaat ttttgatgca ggatttgaaa
     4621 taggcgaagt tacttgccct acaaaatatt ttgaggatgc ttcttctata aatcttaaaa
     4681 gaagtattac ctatggttta ggtgttttaa aagtttcgtt tgggtatttt ttcaagaaac
     4741 ttggcttaaa taattcagtt atttacaatc ataaaaaata aaagcaattt tattaagctt
     4801 actattttct acccaaaaat atttatatgg atgtacaaat agaagccagt tggaaagcag
     4861 ttttgcagca agagtttacc aaaaactatt ttcaacaaat tgttgcacat cttaaaacag
     4921 aaaaagcaac taaaactatt atttatccaa gtggctcatt aatttttaat gcatttaaac
     4981 aaactccatt taatcaggtt aaagtagtaa tcttaggtca agacccctat catggtattg
     5041 ggcaagcaat ggggttaagt tttagtgtgc caaatggagt ttctaatcca ccatctttac
     5101 aaaatatttt taaagaatta caaactgata ttggaatgcc tattccaagc tctggcaatt
     5161 taactgcatg ggctaaacaa ggtgttttgc ttttaaatgc tgtgttaact gttagagcca
     5221 acgaagcagc aagccatagt aaaattgggt ggacaaactt tactgatgct gtaattcaaa
     5281 aattatctga tgaaaaaata catcttgttt ttctactttg gggaaaattt gcacaagaaa
     5341 agcaaatatt aattgatgaa actaaacatt ccgtattaaa agctgctcat ccatcgccat
     5401 tgagtgctta caacggcttt tttggctgta aacattttag cagaactaat gaaatacttg
     5461 ttaaaaatgg cgttgagcct ataaactgga gtttttaaaa ttttatatta tatgaaacaa
     5521 ttacttgaat tttttaaaaa tgtttttgcc agaatttggg cattatgggg tttggtaact
     5581 tttgttgtta ccttttttat tttctttatt ccatcaatgt gtacccactt aattgcagat
     5641 gtaaataaaa gccaaaatgt ttttatttat atagcaaaaa tttggatgaa tgtttggctg
     5701 tttttagttg gctgtagggt aaaagttaga ggtaaagaga atgttgaaaa aaataaaact
     5761 tatattttta catgcaacca taacacttta ttagatgtac caataacttg cccatatata
     5821 ccaggcggaa ataaaacaat tgctaaaaaa agttttgtaa aaattccaat ttttgggtgg
     5881 tattatgcta aaggaggaat attgattgat agaaaaagtg atgcaagtag aagaatggga
     5941 tttgaacaaa tgaaaaaaac tttgcaacaa aaaattcatg tttgtattta tcctgaagga
     6001 acaagaaaca gaacaggtaa gcctttgaaa agcttttatg atggtgcatt taaacttgca
     6061 gaacttgcac aaacaccaat agttccaact atgttataca atacattaaa ggctatgcct
     6121 aacaataagt tcttttattt gttaccccat agtttagaga tacatttttt accagctatc
     6181 agtcctcaaa atttaactat gaaagaactt aaagataagg tttttgatat tatgtataaa
     6241 gaatttgaac ttaataataa aaaaaaaact gtttcaattt tttatttatt gaaacagtta
     6301 acaagtattt ttataattac ttaaatctgc tactcacaac taaatctttg ctaccggctg
     6361 tgtaagtata aaatccttcg ccagatttta cgcctaattt gccagctgtt accatgttta
     6421 ctaataaagg gcatggagca tattttgtat ttccataacc attatacaat acgtttaaaa
     6481 tgcttaagca tacatctaaa ccaataaagt cggctaattg taaaggtccc atagggtgtg
     6541 ccattcctaa cttcataata gtatcaattt cttccacacc tgcaatacct tcgtataaac
     6601 taataattgc ctcattaatc ataggcatta atattttgtt tgcaataaac cctggataat
     6661 catttacaac acatggtatt ttacccaatt gtttacttaa cgcaaca
//
LOCUS       DAOQHP010000078         6664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6123_length_6664_cov_3.773945, whole genome shotgun sequence.
ACCESSION   DAOQHP010000078 DAOQHP010000000
VERSION     DAOQHP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6123_length_6664_cov_3.773945"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLY81_03785"
     CDS             complement(<1..838)
                     /locus_tag="PLY81_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF29378.1"
                     /translation="MTTNTAVLSIQNLVKYYGSIKALDGISYDIPKGCVYGILGPNGS
                     GKTTTLGIVLNVLNQTSGTYLWFGNPANADTRKKIGSLLETPNFYHYLSAVDNLKITQ
                     SINKRGTEEDIHKVLEIVNLAQRKNSKFSTYSLGMKQRLAIAAALLGNPEVLVLDEPT
                     NGLDPVGIAEIRELIKSLAENGKTIIMASHLLDEVEKVCTHVAIMKKGVLITAGAVKD
                     VLVNEDIVEISCANNEKLVEIMKLFPEIQNIQIEKDKLLLYFLLGKANLEAINQYCFS
                     NNI"
     gene            complement(864..2114)
                     /gene="pepT"
                     /locus_tag="PLY81_03790"
     CDS             complement(864..2114)
                     /gene="pepT"
                     /locus_tag="PLY81_03790"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNF29379.1"
                     /translation="MIANYKHTVVERFLEYVQIDTQSNPNSTTHPTTEKQKNLSNLLA
                     QQLVLLGLQDVVADEYGYVYATIPSNTTKKVPVICFCSHVDTAPDCSGNNVKPIFHAN
                     YSGNDIILPDDDNQILSIQKYPYLKEHIGNSIITASGKTLLGADDKAGVAIIMDMVNY
                     LTTHSSIKHGTIKILFTPDEEVGQGTAKINLEKLGADFAYTLDGGELGTLEDETFSAD
                     GATITVHGVIAHPGYAKNKLVNALKIVGEILASLPENEFSPETTDGRQGFVHPTAIEG
                     IAEKASINFIVRDFETSKLKEHEQRLEAIAQKVVAKYKGATMQMDVFEQYRNMKEVLD
                     KYPQVVEHAAKAYKRAGLNVINEPIRGGTDGSRLSFMGLPCPNLFTGMQAIHSKNEWI
                     GVKDMEKAVEVLVHLAQIWEEEGV"
     gene            complement(2238..2969)
                     /locus_tag="PLY81_03795"
     CDS             complement(2238..2969)
                     /locus_tag="PLY81_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF29380.1"
                     /translation="MNKLTDSWFSPALNKEMPIVSYGFNGFAVLLVPTAAADYLEYER
                     FQMLEALAPLIDKGKLRVFSINSINNESWLNNEILPEHKAIRHNQFNDYVFNEVVPFI
                     RNMAGADTFIYTSGASFGALHSMNLFLKRPDIINGCISMSGVYDLTEYTKGFWDEQVY
                     FNSPFHYVPNLTDTWYIDKIKASHHIHIATGSGNYEDPEANKKFSQVLWNKGIWHDLD
                     VWGPDIHHDWPTWRAMLPYILEVKF"
     gene            complement(3066..3153)
                     /locus_tag="PLY81_03800"
     tRNA            complement(3066..3153)
                     /locus_tag="PLY81_03800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3117..3119),aa:Ser,seq:gga)
     gene            complement(3197..3628)
                     /locus_tag="PLY81_03805"
     CDS             complement(3197..3628)
                     /locus_tag="PLY81_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNF29381.1"
                     /translation="MNQPSNNFLTAVLKNKCPRCRQGNLFLNKNPYKLKDGLLMHEKC
                     LVCGQPTEIEVGFYYGTGYLSYAISFAFLVATMVAWAVIFGLSFKDNSIFEWLITACI
                     LLVMLQPILMRLSRTLWLSFFVKYDANWKNNPVDKPERFIK"
     gene            complement(3663..4934)
                     /gene="murF"
                     /locus_tag="PLY81_03810"
     CDS             complement(3663..4934)
                     /gene="murF"
                     /locus_tag="PLY81_03810"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNF29382.1"
                     /translation="MKIEELYHIYCNHSIICTDTRKIESNCIFFALKGENFNGNQFAL
                     EALNNGAAFVVVDEQVGISNDKIILVENVLYTLQQLAKYHRQQFTIPFIAITGSNGKT
                     TTKELVSAVLKTQYTTYTTQGNLNNHIGVPLTILSIKKDAEMAVIEMGANHQKEIESY
                     CLYTLPTHGIITNCGKAHLEGFGGEEGIKKGKGELFSFLKEHNGTAFVYNEYDYLLEM
                     AMGIKEIVEYGSSSTLFSASIVQFDPYLSIKFSTNVTTEICTNLVGKYNFSNVLTAVA
                     IGLYFNISMDKIKNAIVQYKPSNSRSQLLKIESNYFILDAYNANPSSMKVALENFAAI
                     KADNKVVMLGAMMELGIDSIKEHQQIADLLLQLKFKNVVLTGGDFKFTHHPFIYKENN
                     EAAKLWLQQNKFTNTYFLIKGSRSMQMEKIL"
     gene            5059..5517
                     /locus_tag="PLY81_03815"
     CDS             5059..5517
                     /locus_tag="PLY81_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF29383.1"
                     /translation="MKLEKAIQSSKFKSEVQKAGLNILYTAWWLKTATSKELKLFGLT
                     HEQYNVLRILKGKHPEQMCVKDIAGRMIEKNSNVPRIIDRLVAKKLVKRSQCCNDGRQ
                     TVMELTTQGILILNNSNNKLDTLFENLMSLNNKEAQALNQLLEKLRENEL"
     gene            5567..6427
                     /locus_tag="PLY81_03820"
     CDS             5567..6427
                     /locus_tag="PLY81_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNF29384.1"
                     /translation="MKTVKKIYSGKATNVGSIKVQELLPSIDGFLDPFLVLHHGIAIA
                     DENLPLKEQGVGPHPHRGFSPVSFIYKGGIHHRDSRNNNSVVYKGGAQWINAGMGIIH
                     SERLPEDILAHGGKQEIIQFWINTPAKFKMQQPAYFPVPSEEMPTIHSEDGLTTLRVP
                     AGKFENIKGTIPTFSDVTTIMGDIKKEGTYNFTFNTQHNSLLYVLEGELLVNEQTKIS
                     SKQLAVFNNDGEIFTIKANENTLFFVASGEPLNEPIAAHGPFVMNTQTEILEAFKDYQ
                     LGKMGVLIEE"
     gene            6466..>6664
                     /locus_tag="PLY81_03825"
     CDS             6466..>6664
                     /locus_tag="PLY81_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF29385.1"
                     /translation="MLTYKIDAAHSEITFKVKHLMITNVTGNFTNFNASLTTTKEDFS
                     DAEITFEAATNSINTNNEQRDA"
BASE COUNT         2281 a         1126 c          946 g         2311 t
ORIGIN      
        1 caatgttatt actaaaacaa tattgattta tagcttctaa atttgcttta ccaagtaaaa
       61 aataaagtag cagtttgtct ttttctattt gtatattttg aatttcggga aagagtttca
      121 ttatttctac taatttttca ttattagcac aagaaatttc aacaatatct tcatttacca
      181 atacatcttt tactgcccca gcagttatta aaacaccttt tttcatgata gctacatggg
      241 tgcatacctt ttcaacttcg tctaacaagt ggcttgccat aataatagtt ttaccatttt
      301 ctgcaagact tttaattaat tctctaattt cagcaatgcc tacaggatct aaaccattag
      361 ttggttcatc taaaactaaa acctctggat taccaagtaa agcagcagca atagccaacc
      421 tttgtttcat acccaaacta taggtactga atttgctgtt tttcctttga gctaaattta
      481 ctatttctaa tactttatgt atatcttctt ctgttcctct tttgtttatg gattgagtaa
      541 tttttaaatt atcaaccgca gacaagtaat gataaaaatt aggtgtctct aataagctcc
      601 caattttttt tctggtatcg gcattagcag ggtttccaaa ccataaataa gtaccactag
      661 tttggtttag tacattaaga acaataccta aggttgttgt tttgccacta ccattggggc
      721 ccaaaatacc ataaacacag ccttttggaa tatcgtaaga tataccatct agggctttta
      781 tggagccata atattttact aaattttgta tggataaaac agctgtattt gtagtcatat
      841 tattaattaa ttgtcttata aaatcacact ccttcttctt cccaaatttg agcaaggtgt
      901 accaaaactt ctacagcttt ttccatatct tttacaccaa tccattcatt tttgctatgt
      961 attgcctgca tacctgtaaa taaatttggg cagggtaagc ccataaaact taaacggctg
     1021 ccatctgttc ctcctcgtat tggctcatta ataacattta atcctgcacg tttataagct
     1081 ttggctgcat gctcaactac ttgtggatat ttatctaata cttctttcat atttctatat
     1141 tgttcaaaaa catccatctg catggttgcc cctttatatt tagcaacaac tttttgtgca
     1201 attgcttcta atctctgttc atgctctttt agtttgcttg tttcaaaatc tctaacaata
     1261 aaattaatgc ttgctttttc tgcaatacct tcaatggctg ttgggtgtac aaatccttgt
     1321 ctgccatctg ttgtttctgg actaaattca ttctcgggta aactagctaa aatttctcct
     1381 acaattttta gagcatttac taatttattt tttgcataac caggatgtgc aattacacca
     1441 tgaacagtaa tggttgctcc atctgcacta aaggtttcat cttctaaagt acccaattct
     1501 cctccatcca aagtataagc aaaatctgcc ccaagttttt ctaaattaat ttttgcagtt
     1561 ccttgtccta cttcttcatc tggtgtaaat aatattttta ttgtaccatg ttttattgat
     1621 gaatgtgtag ttaagtaatt caccatatcc ataataattg ctacaccagc tttatcatcg
     1681 gcacctaaca atgttttacc acttgctgta attattgagt tgccaatatg ctcttttaaa
     1741 tatggatatt tttggataga taaaatctgg ttatcatcat ctggtagaat tatatcattt
     1801 cctgagtagt tagcgtgaaa aataggtttt acattatttc cgctacaatc tggtgcagtg
     1861 tctacgtggc tgcaaaaaca tattacaggt acttttttag ttgtgttaga tggtatggtt
     1921 gcatatacat agccatattc atctgcaact acatcttgta aacccaataa tactaattgt
     1981 tgggctaata agttggataa gtttttttgc ttttctgttg taggatgggt agtactattt
     2041 ggattacttt gtgtatctat ttgcacatat tccaaaaacc tttcaacaac tgtatgttta
     2101 taatttgcta tcattaataa taataattta gggcaagata aaataaagca ttcttaaata
     2161 aatgcctttg gtgtaatttt taataaaaag agtaaagtgg ttgattactt tactctataa
     2221 gatatttata aaaaatttta aaacttaacc tctaaaatat aaggcaacat tgctctccaa
     2281 gtaggccagt catgatgaat atctgggccc catacatcta aatcatgcca aattccttta
     2341 ttccacaaaa cttggctgaa ttttttattg gcttcaggat cttcgtaatt accactacct
     2401 gttgcaatat gtatatgatg gcttgcttta attttatcaa tataccaagt atctgttaag
     2461 tttggcacat aatgaaaagg gctgttaaaa taaacttgtt cgtcccaaaa gcctttggtg
     2521 tactctgtta aatcatacac tccactcatg ctaatacaac cattaataat atctggcctt
     2581 tttaaaaata aattcatact gtgcaatgct ccaaaacttg caccactggt ataaataaaa
     2641 gtatctgcac ctgccatatt tctaataaaa ggtactactt cgttaaaaac ataatcatta
     2701 aactgattat ggcgtattgc tttatgctct ggcaatattt cattatttaa ccaactttca
     2761 ttattaatac tgttaatgct aaaaactcta agttttcctt tatctattaa tggtgccaat
     2821 gcttctaaca tttgaaacct ttcgtattct aaataatctg cagctgcagt aggtactaat
     2881 aatacagcaa aaccattgaa tccatacgac acaataggca tttccttatt taaggctggg
     2941 ctaaaccatg aatctgtaag tttattcata acagttaatt ttcaattaaa gaataagata
     3001 tatttataac aatgttatca aaaaaaattg aaataaatag gaattaataa attggaataa
     3061 ggaagcagag gggaagggat tcgaaccctc gattcgcttt agacgaatac acactttcca
     3121 ggcgtgctcc ttcaaccact cggacacccc tcttttaaaa agtaggcaaa tgtaaagttt
     3181 ctactcttaa tgaaagttat tttataaatc tttctggctt atctacaggg ttatttttcc
     3241 aattagcatc atatttcaca aaaaaactga gccaaagtgt tctagataat cgcattagaa
     3301 ttggttgcag cattactagc agtatgcaag cagtaataag ccattcaaaa atactattat
     3361 ctttaaaaga caatccaaat atcacagccc atgctaccat tgttgccact aaaaacgcaa
     3421 aagaaattgc ataacttaaa tagcctgtgc cataatagaa accaacttca atttcagttg
     3481 gttggccaca aaccaaacat ttttcatgca ttaaaagacc atctttaagt ttatatgggt
     3541 ttttgtttaa aaacaagttt ccttgtctgc atcttgggca tttattctta agtacagcag
     3601 ttaagaaatt attagagggc tggttcatac cacaaagtta aatttttatt tgggcaaaat
     3661 agttataaaa ttttttccat ctgcatagat ctcgaccctt taattaagaa ataagtgttg
     3721 gtaaatttat tttgctgcag ccacaactta gctgcttcat tattctcttt ataaataaat
     3781 ggatgatggg tgaatttaaa atctccgcct gttaatacaa catttttaaa ttttaactgc
     3841 aataataaat ctgcaatttg ttgatgttct tttatgctgt caattcctaa ctccatcata
     3901 gctccaagca ttacaacttt attatctgcc ttaattgcag caaaattttc taaagccact
     3961 ttcatactac ttggattggc attgtatgca tctaaaataa aataattact ctctattttt
     4021 aataactggc tcctgctgtt agatggtttg tattgtacaa ttgcattttt tattttatcc
     4081 atagatatat taaaatacaa accaatggca acagccgtta aaacatttga gaaattatat
     4141 tttccaacca aattagtgca aatttctgta gtaacatttg tgctaaattt aatagacaag
     4201 taagggtcaa actgaacaat gcttgcagag aataatgtcg atgaagaacc atattcaact
     4261 atttctttta ttcccatagc catctctaat aaataatcat actcattata aacaaatgca
     4321 gtaccattat gttcttttaa aaaagaaaac agttcccctt tccctttttt tattccttct
     4381 tctccgccaa aaccctctaa atgtgcttta ccacaatttg ttataatgcc atgtgttggc
     4441 aatgtatata aacaatagct ttctatttct ttttgatggt ttgcccccat ttctatcaca
     4501 gccatttcag catctttttt aatacttaaa atagttaagg gcacgccaat atgattgttt
     4561 aaattgcctt gtgtagtata tgttgtgtac tgagttttta aaacagcact aaccaattct
     4621 ttagttgttg ttttaccatt gctgcctgtt attgcaataa aaggaatggt aaactgttgc
     4681 cgatggtatt ttgctaattg ttgtaaagta tataaaacat tttcaaccaa aataatttta
     4741 tcattgctta tacctacttg ctcatctacc acgacaaaag cagctccatt attcaaagct
     4801 tctaatgcaa attggttacc attaaaattt tctcccttta aagcaaaaaa aatacaattt
     4861 gattcaatct ttcttgtatc ggtacaaata attgaatgat tgcaataaat atggtataac
     4921 tcttctattt tcaaatttat attacatttt atatcacaaa aataagcaga tttaacactg
     4981 aagattttgt tgatttattt gtttgaaaaa ataaaatttg gaaatacatg tacatacatg
     5041 tatatttgca ttttagaaat gaaattagaa aaagcaatac aaagtagtaa atttaaaagt
     5101 gaagtgcaaa aagcagggtt gaatatatta tatacagctt ggtggttaaa aacagcaaca
     5161 tccaaagaat taaaattatt tggcttaact cacgaacaat ataatgtatt aagaatatta
     5221 aaaggtaagc atcctgaaca aatgtgtgtt aaagatattg ctggtagaat gattgaaaaa
     5281 aactctaacg tacccagaat tattgacaga ttggtggcaa aaaaattagt taaacgaagc
     5341 caatgctgta atgatggaag acaaacagta atggagttaa ctacacaagg aattttaatt
     5401 ttaaataatt cgaataataa attagatact ttgtttgaaa atttaatgtc attaaataat
     5461 aaagaagccc aagcattaaa ccaactactt gaaaaactaa gagaaaacga attgtaattt
     5521 ttttataaaa ttacatgtac atacatatat ttaaaaaaca aacaaaatga aaacagttaa
     5581 aaaaatttat tcaggaaaag caaccaatgt tggcagtata aaagtgcaag agttgttgcc
     5641 atctatagat gggtttttgg atcccttttt agtactacat catggtattg ccattgcaga
     5701 tgaaaatttg cctttaaaag aacaaggtgt tggcccacac ccccatagag gttttagtcc
     5761 tgtaagcttt atttacaaag gaggcattca tcatagagat agtagaaata acaatagtgt
     5821 tgtgtacaaa ggtggtgcac aatggataaa tgcaggtatg ggaataatac acagtgaaag
     5881 gcttccagaa gatatattag cacatggagg taaacaagag ataattcaat tttggattaa
     5941 tacacctgca aaatttaaaa tgcaacaacc agcctatttt cctgttccct ctgaggaaat
     6001 gcctaccata cattcagaag atggattaac aaccttaaga gtacctgctg gaaaatttga
     6061 aaatataaaa ggtactattc ctactttttc ggatgttact actataatgg gcgacataaa
     6121 aaaagaagga acttacaact ttacttttaa tacccaacac aatagtttac tgtatgtatt
     6181 agagggcgaa ttattagtga atgaacaaac aaaaatatcc tcaaaacaat tagctgtttt
     6241 taataacgat ggggaaatat ttacaataaa agctaatgaa aatactttgt tttttgttgc
     6301 aagtggcgaa ccattaaatg agccaattgc tgcacatgga ccatttgtaa tgaataccca
     6361 aacagaaatt ttagaagctt ttaaagatta tcaattaggc aaaatgggcg tattaattga
     6421 agaataaaat ttaattacat tatttataca ctaaaaaaac aaactatgtt aacttacaaa
     6481 attgatgctg cacacagcga aattactttt aaagtaaaac acttaatgat taccaatgtt
     6541 acaggtaatt ttaccaactt caatgcatct ttaacaacca ctaaagaaga ctttagtgat
     6601 gcagaaatta cttttgaagc agcaaccaat agtattaata ctaataacga acaaagggat
     6661 gcac
//
LOCUS       DAOQHP010000079         6638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6170_length_6638_cov_3.661552, whole genome shotgun sequence.
ACCESSION   DAOQHP010000079 DAOQHP010000000
VERSION     DAOQHP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6170_length_6638_cov_3.661552"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PLY81_03830"
     CDS             <1..145
                     /locus_tag="PLY81_03830"
                     /inference="COORDINATES: protein motif:HMM:NF040139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HNF29386.1"
                     /translation="EKAGGKATNGKQRILDIQPTELHQRTPFFIGSSSMMDELETYLA
                     ANK"
     gene            207..1118
                     /locus_tag="PLY81_03835"
     CDS             207..1118
                     /locus_tag="PLY81_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNF29387.1"
                     /translation="MPTVKNYLSLIKFSHTIFALPFAMIGFVLGIKTVDEFNKSLLIG
                     KLQLNNFWSFFAIKLLLVLVCMVSARSAAMAFNRYLDRNFDSKNPRTAIREIPSGIIA
                     PNSALRFVIVNCLIFITATFFINDICLYLSPIALLVILFYSYTKRFTALCHLVLGIGL
                     SLAPIGAYLAITGAFSFIPLLFSFAVIFWVSGFDIIYALQDDEFDKAYKLHSIPAALG
                     KKKALKVSELLHFFCTICVIWAGWLGSFSWLYWLGVCIFIAMLVYQHSIVKPNDLSKV
                     NIAFMTANGIASVVFAVFVIADLLILA"
     gene            1178..1657
                     /locus_tag="PLY81_03840"
     CDS             1178..1657
                     /locus_tag="PLY81_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29388.1"
                     /translation="MKKLFTFLLVTLVVFSSNSLKAQDWKLGVSIMPGVASNGGYGFV
                     LGSDARLQTKLMPKMHFILTTGVTQYFKKSPLSGFGYIPLKPGVKYFWGDNFYTAGEI
                     GLGFGLVKGSGRSFIWSPSVGLAFKKVDVSLKYEDTPDFGRYGKHFAIRVARGFDLK"
     gene            1669..2082
                     /gene="ruvX"
                     /locus_tag="PLY81_03845"
     CDS             1669..2082
                     /gene="ruvX"
                     /locus_tag="PLY81_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNF29389.1"
                     /translation="MARIICIDYGAKRSGIAVTDPLQIIATALTTINTSNLLAFLQNY
                     FTQEEVELILVGEPLGLNDEATHATPLVYAFVKNLKKQFPLIPVKLYDERFSSKMAVQ
                     AMIEMGMKKKERKIKGNIDQIAATIMLQEYLANKT"
     gene            2136..2714
                     /gene="def"
                     /locus_tag="PLY81_03850"
     CDS             2136..2714
                     /gene="def"
                     /locus_tag="PLY81_03850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNF29390.1"
                     /translation="MIFPIVAYGASVLRKKALAIDSQYPQLNTLISNMWETMYASNGV
                     GLAAPQINKDIRLFVIDSSQIFENMEEAEKKNYPDSPGLKKVFINAQIKDLDGEEWSF
                     NEGCLSIPKIREDILRPEMVVLEYMDENFETHIEAFHGITARVILHEYDHIEGKLFID
                     YLKPLKKKLLQGKLNDIMKGKVKVDYKMTFIK"
     gene            2711..3649
                     /locus_tag="PLY81_03855"
     CDS             2711..3649
                     /locus_tag="PLY81_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29391.1"
                     /translation="MKWFAVLIGCCCNNILHAQDSVIVIDKKKFTLTEVVVRNNFDYK
                     KILQQIKEDTTFYKAFKNLRILGYTAFNDIKMLNKKDKIIASLFSKTIQTRNNNCRTM
                     QVLEENVTGNFYDKNGNFNYTTANMYASLFFTNGTVCNENNIVAVNTFSTANKKGMEK
                     HKEQLKMLFFNPGKKIPGIPFIGNKIDLYDNAAQKIYNYTLDLQEKGGKIYYVFSIIP
                     KEKLGLFKADNVVIDEMVTWFDQKTMEVVYRSYSLSYNAGVYDFDVNMEVEMTNHNGY
                     IVPKTLRYRGNWDVMFKKREKALFTATLFNFKSEGE"
     gene            3779..4030
                     /locus_tag="PLY81_03860"
     CDS             3779..4030
                     /locus_tag="PLY81_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNF29392.1"
                     /translation="MKQGIHPESYRLVIFKDMSNGHAFLSKSTAATKETLKWEDGNEY
                     PLIKLDISNTSHPFYTGKNVMVDTAGRIDKFKKRYEKKK"
     gene            4119..4847
                     /gene="bshB1"
                     /locus_tag="PLY81_03865"
     CDS             4119..4847
                     /gene="bshB1"
                     /locus_tag="PLY81_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNF29393.1"
                     /translation="MKLDILAFGVHPDDIELGCAGTLLAAMAEGKKVGIIDLTQGELG
                     TRGTAETRKVEAENAAKILNVQIRENLKMADGFFKNDEAHQRKVIETIRKFKPEIVLC
                     NAPEDRHPDHGRSSKLVSDASFLSGLIKIVTNDNGKLQEAWRPKYVFHYIQDRFLQPD
                     FVVDISAHQATKMQAIACYTTQFFNTDSDEPQTYISNPVFLETVKARAMMLGKRIGVE
                     YAEGFISEKVIGINSFDAFIQNVT"
     gene            4900..6144
                     /gene="hutI"
                     /locus_tag="PLY81_03870"
     CDS             4900..6144
                     /gene="hutI"
                     /locus_tag="PLY81_03870"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNF29394.1"
                     /translation="MSATLITNIKFLVNATETPALLKGKDLANLPVLENAYLIISNKK
                     IVAYGVMEDFLHHTQTIKEVIDAEGQMVLPCWCDSHTHLVFAASRENEFVDKLKGKTY
                     AEIAASGGGILNSAKRIAYSTEQELFDSAYNRLHELIKLGTGAIEIKSGYGLSVESEL
                     KMLRVIKKMKEKSPIPIKATFLGAHTYPLKFKEDHKGYIDLIINEMLPAIAKENLADY
                     IDVFCEEGFFSKEETITICKAGMQYGLKPKIHANQLNLSGGVQAGRELNALSVDHLET
                     MNDEAINLLAKSNTIGTLLPTAAYFLRMPFQPARKLIDAGCTIALASDFNPGSSPSGN
                     MNFVVSMSCIQMKMLPEEAINAATINGAAAMELQNEVGSITKGKIANLIFTKPIPSIA
                     YMPYAFGSNLIDKVMINGSFYK"
     gene            6252..>6638
                     /locus_tag="PLY81_03875"
     CDS             6252..>6638
                     /locus_tag="PLY81_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNF29395.1"
                     /translation="MRSIAFREALREAMSEEMRRDERVFLMGEEVAEYNGAYKVSQGM
                     LAEFGAKRVIDTPIAELGFAAIAVGAAQNGLRPIVEFMTWNFAVLPLDQIFNTASKML
                     AMSGGQIKCPIVFRGLNASAGQLGAQH"
BASE COUNT         2410 a          871 c         1161 g         2196 t
ORIGIN      
        1 tgaaaaagca ggtggcaaag caaccaatgg taaacaaaga attttagata tacaaccaac
       61 cgaattacac caacgtactc ctttttttat tggaagtagt agtatgatgg atgagttaga
      121 aacctacttg gctgccaata aataattaaa aaataccaac agttagaatg tgttttgtac
      181 aatagaatac attctatttt ttatgtatgc ctactgtaaa aaactatcta tccttaatta
      241 agttttctca taccattttt gcattaccat ttgcaatgat aggttttgtg ttgggcataa
      301 aaacggtgga tgaatttaat aaatcacttt taataggtaa attacaactt aataattttt
      361 ggtctttttt tgccataaaa ctgcttttgg tattggtttg tatggtgagt gcaaggtcgg
      421 cagcaatggc atttaataga tatttagata gaaattttga ttctaaaaac ccacgaacag
      481 ccattagaga aataccaagt ggtattatag caccaaacag tgcattgcgg tttgtaattg
      541 ttaattgcct aatttttatt actgctactt tttttattaa tgatatttgt ttatacttat
      601 caccaatagc cttactggta attttatttt acagttatac aaaaagattt acagcattat
      661 gccatctagt attaggcatt ggtttatcct tagcacctat aggagcttat ttagctataa
      721 ctggtgcatt tagttttatt cctttactat tttcatttgc tgttattttt tgggtaagtg
      781 gttttgatat tatttatgct ttacaagatg atgagtttga taaagcctat aaacttcact
      841 ctataccagc agcattaggt aaaaagaaag ctttaaaagt atccgaatta ttacactttt
      901 tttgtactat atgtgtaatt tgggcaggtt ggttaggtag ttttagttgg ttgtattggt
      961 taggtgtatg tatttttatt gcaatgttag tgtaccaaca ttctattgtt aaacccaacg
     1021 acttaagtaa agtaaacatt gcatttatga ctgccaatgg cattgcaagt gttgtttttg
     1081 ctgtatttgt tattgccgat ttattgattt tagcttaata ctttttacat tttctaaaaa
     1141 aaatgtactt tcgccgttaa tcttataata atttgatatg aaaaaattgt ttactttttt
     1201 acttgttacg ttagtagtat tttcttccaa ttcattaaaa gcacaagatt ggaaattagg
     1261 ggttagtatt atgcctggtg ttgcatcaaa cggaggatat ggatttgttt tgggttctga
     1321 tgcaaggtta caaaccaagc taatgcctaa aatgcatttt attttaacta ctggggtaac
     1381 tcaatatttt aaaaaaagtc cactatctgg ctttggctat atacctttaa aaccaggtgt
     1441 taaatacttt tggggcgata atttttatac tgctggcgaa attggtttag gttttggttt
     1501 agtaaaaggt agcggtagat cttttatttg gtctccatct gttgggttgg catttaaaaa
     1561 ggtagatgtt agcttgaaat atgaagatac accagacttt ggacgatatg ggaaacactt
     1621 tgctataaga gtagcaaggg gttttgattt aaaataattt tacaactaat ggctagaatt
     1681 atttgtattg attatggtgc caaaagatct ggtattgctg ttaccgatcc attacaaata
     1741 attgcaacag cattaacaac tataaataca agtaacctac ttgcattttt gcaaaattat
     1801 tttacccaag aagaagttga attaatattg gttggcgaac ctttaggttt aaatgatgaa
     1861 gcaacacatg caacaccatt ggtttatgca tttgtaaaaa atttaaaaaa acaattccca
     1921 ttaataccag ttaaacttta cgatgaaagg tttagttcta aaatggctgt tcaagcaatg
     1981 attgaaatgg gaatgaaaaa aaaagaaaga aaaattaaag gcaatataga ccaaatagct
     2041 gctactataa tgttgcaaga atatttggct aacaaaacat aatatagctc ttttttaaaa
     2101 taattaaaag ttataaatat aaaatagttt aacaaatgat ttttcccata gtggcttatg
     2161 gtgcaagtgt attaagaaaa aaggctttag caatagatag ccaatatcca caattaaata
     2221 ctttaatcag caatatgtgg gagaccatgt atgctagtaa tggcgttggt ttagctgccc
     2281 ctcaaataaa taaagatatc aggctttttg taattgacag ttctcaaatt tttgaaaata
     2341 tggaggaggc agagaagaag aattaccctg attctcctgg tttaaaaaaa gtatttataa
     2401 atgcacaaat aaaagattta gatggagaag agtggtcgtt taacgaaggc tgtttatcta
     2461 ttcctaaaat aagagaagat attttaagac cagaaatggt tgtgttagaa tatatggatg
     2521 aaaattttga aacacatata gaagcttttc atggtattac agctagggtt attttacatg
     2581 aatacgacca tatagaaggt aaattattta tagattattt aaagccttta aagaaaaaac
     2641 tattacaagg caagttaaac gatatcatga aaggcaaagt aaaagtagat tacaaaatga
     2701 catttattaa atgaaatggt ttgctgtttt aataggttgt tgctgtaata atatcctaca
     2761 tgctcaggat tcggtgattg taattgataa aaaaaaattc actttaacag aagttgtagt
     2821 tagaaataat tttgactaca aaaaaattct gcaacaaatt aaagaagata ctacttttta
     2881 taaagccttt aaaaatttac gtatattagg ttacactgcg ttcaacgata ttaaaatgtt
     2941 gaataaaaaa gataaaatta ttgcttcgtt gtttagtaaa accatacaaa caagaaataa
     3001 taattgcaga actatgcaag tgttagaaga gaatgtaaca ggcaattttt acgataaaaa
     3061 tggcaacttt aattatacaa cagctaatat gtatgcttcg ttatttttta caaatggaac
     3121 agtttgtaat gaaaacaata tagttgcagt taataccttt tctactgcaa acaaaaaagg
     3181 tatggagaaa cataaagaac aacttaaaat gttatttttt aatccaggta aaaaaattcc
     3241 aggaatacca tttattggca ataaaattga tttatacgat aatgctgcac aaaaaattta
     3301 taattatact ttagatcttc aagaaaaagg agggaaaatt tattatgtat tttctattat
     3361 tcctaaagaa aaattagggt tgtttaaagc agataatgtt gttattgatg aaatggttac
     3421 ttggttcgat caaaaaacca tggaagtggt gtataggagc tattctttaa gttataatgc
     3481 aggggtttat gattttgatg taaatatgga agtagaaatg accaaccata atggttatat
     3541 tgtacccaaa acattaagat atagagggaa ttgggatgta atgtttaaaa aaagagaaaa
     3601 agcattattt actgcaactt tattcaactt taaaagcgaa ggagaatagc acttatttta
     3661 atattgattc tcagctattt ctataccttt ttggatatga atgttgtagc ccctaaattt
     3721 gcaaactatt attatttaat tgacacggca aaatccgtgc agccttaaaa aaaaatttat
     3781 gaaacaaggt atccatccag aaagttatcg tttagtaatt ttcaaagaca tgagtaatgg
     3841 gcatgcattt ttaagcaaat caactgctgc aactaaagaa acacttaaat gggaagatgg
     3901 taatgagtat cctttaatta aattagatat ctctaataca tctcatcctt tttacactgg
     3961 taaaaatgta atggttgaca ctgcaggacg tattgataaa ttcaaaaaac gttacgaaaa
     4021 gaaaaaatag ttttcattgt tttttttatt ttagaaaaat cccgttccta aaaacgggat
     4081 ttttcttttt cattattttt atcaaaattt tagaataaat gaaattagat attttagcat
     4141 ttggtgtaca ccccgatgat attgaattag gctgtgcagg aacattatta gctgcaatgg
     4201 cagaaggtaa aaaagttggt attatagatt taactcaagg agaattagga acaagaggta
     4261 cagcagaaac cagaaaagtg gaggctgaaa atgcagccaa aattttaaat gtacaaataa
     4321 gagaaaactt aaaaatggcc gatggctttt ttaagaatga tgaagctcat caaagaaaag
     4381 ttattgaaac aataagaaaa tttaaaccag aaatagtatt gtgcaatgca ccagaagata
     4441 ggcacccaga tcatggaaga agctccaaat tagttagtga tgcatctttt ttaagcggtt
     4501 taattaaaat tgttacaaac gacaatggta aattacaaga agcctggcgt cctaaatatg
     4561 tgtttcatta tatacaagat agatttttgc aacctgattt tgtagtagat atatctgcac
     4621 atcaggcaac aaaaatgcaa gccattgctt gttatactac acaattcttt aatacagatt
     4681 ctgatgaacc tcaaacgtat atttctaacc cagtattttt agaaactgtt aaagcaaggg
     4741 caatgatgtt ggggaaaaga attggtgtag agtatgcaga aggatttata tctgaaaaag
     4801 taataggaat aaattctttt gatgccttta ttcaaaatgt aacttaatat tacaattaat
     4861 ttgaaaattg atttatcatt ataaaaagtg tataagaata tgtctgctac cttaataacc
     4921 aatataaaat ttttggttaa tgcaaccgaa acaccagcct tattaaaagg aaaagattta
     4981 gcaaatctgc ctgttttaga aaatgcatat ttaattattt ctaacaaaaa aatagttgct
     5041 tatggtgtaa tggaagattt tttacatcat acacaaacca ttaaagaagt aatagatgca
     5101 gaaggacaaa tggttttacc ttgttggtgc gatagccaca cacatttagt atttgcagca
     5161 agtagagaaa atgaatttgt agataaatta aaaggcaaaa cctatgcaga aattgctgca
     5221 agtggtggtg gaattttaaa ttctgctaaa agaattgctt actctaccga gcaagaactt
     5281 tttgactctg cttataatag gttgcatgaa ttaataaaat taggcacagg tgcaatagaa
     5341 attaaaagtg gttatggact aagtgtagaa agtgaactta aaatgcttcg tgttataaaa
     5401 aaaatgaaag aaaaatctcc tataccaata aaggcaactt ttttaggggc acatacttat
     5461 ccacttaaat ttaaagaaga tcataaaggc tatattgatt taattattaa cgaaatgctt
     5521 cctgccattg ctaaagaaaa tttggcagat tatattgatg tgttttgcga agagggattt
     5581 tttagtaaag aagaaactat taccatctgc aaagctggta tgcaatatgg tttaaagcct
     5641 aaaattcatg caaaccaatt aaacctaagt ggtggcgtac aagcaggtcg agaattaaat
     5701 gccctttcag tagaccattt agaaacaatg aatgatgaag caataaactt attagctaaa
     5761 tcaaatacaa taggtacatt attaccaaca gcagcttatt ttttaagaat gccttttcag
     5821 cctgccagaa aattaattga tgcaggttgt accattgcac ttgcaagtga ttttaatcct
     5881 ggttcatctc caagtggcaa tatgaatttt gtagtatcta tgagctgtat tcaaatgaaa
     5941 atgctaccag aagaagcaat taatgcagct accattaatg gtgctgcagc aatggaactg
     6001 caaaatgagg ttggcagtat tactaaagga aaaattgcca acttaatttt caccaaacca
     6061 attccatcaa tagcttatat gccttatgct tttggaagta atttaattga caaggtaatg
     6121 ataaatggaa gtttttataa ataataaacc atttctaact ttatttagaa agtatttcaa
     6181 actcaataat actacattgg attatatgca ctaatcaatt acttttgcaa acaccttaat
     6241 ataaaattat tatgcgttca atagcattta gagaagcatt aagagaagcc atgagtgaag
     6301 aaatgagaag agatgaaaga gttttcttaa tgggtgaaga agttgccgaa tacaatggtg
     6361 cttataaagt aagccaaggt atgcttgccg agtttggagc caaaagagta attgataccc
     6421 ctattgccga acttggtttt gcagcaattg ctgtaggggc tgcacaaaat ggtttaagac
     6481 ctatagtaga gtttatgaca tggaactttg cagtgttacc tcttgatcaa atttttaata
     6541 cagcctctaa aatgttggct atgagtggtg ggcaaattaa atgccctatc gtttttcgtg
     6601 gccttaatgc aagtgcaggc cagttaggtg cacaacat
//
LOCUS       DAOQHP010000080         6600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6235_length_6600_cov_3.826891, whole genome shotgun sequence.
ACCESSION   DAOQHP010000080 DAOQHP010000000
VERSION     DAOQHP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_6600_cov_3.826891"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLY81_03880"
     CDS             <1..540
                     /locus_tag="PLY81_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29396.1"
                     /translation="PATEQSTEKDDPDAVATHTNKTTKVTKPVAVAPVNKPKTVMPKY
                     SGGNSNGGNSKDNYNDSRSEGNDKSGVGDKGKPWGSIDGTVYDGPGGTRVYGLGKRRI
                     TRFPTFTDDFSENAKVAINIVVDKNGNVTYAAVNPNGTSTANKQTRAVALKRAKEIKF
                     ETSEQEQRGTIILDMRVSG"
     gene            558..1853
                     /locus_tag="PLY81_03885"
     CDS             558..1853
                     /locus_tag="PLY81_03885"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNF29397.1"
                     /translation="MNYKESVDYLFEQLPMFSRVGGMAIKEGLTNTILICNKLNNPQN
                     KFKSVHIAGTNGKGSVSHMLAAILQTAGFKTGLYTSPHLTDFRERIKVNGKKCSQEFV
                     VQFTEQMKPFIEEFKPSFFEITVGMAFEYFAKEKVDIAIIETGLGGRLDSTNIITPIL
                     SIITNIGLDHVQMLGNSLPEIAVEKAGIIKQNVSVIIGETNAATKTVFIKSAQEKNAS
                     IQFADELFKVNNYKYSIDNLTIEIENIKQHTKEVFTLDLTGLYQLKNIITVIAALKKL
                     QDLDWNINNSHIKTALNKTKQLTGLKGRWQVIRKNNPPVFADVAHNSDGVKQVIQHLQ
                     LLSFNQLHLIVGMVKDKDVSAVLSLLPSNAKYYFTQAQIPRAMPAEDLQKLAAKFNLE
                     GYSFSNVNLAIEYAIQKASKSDLVLIMGSVFLIGEINSI"
     gene            2528..>6600
                     /locus_tag="PLY81_03890"
     CDS             2528..>6600
                     /locus_tag="PLY81_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29398.1"
                     /translation="MKLLNRLTFFVLLFGSLLFNASTVSAQLGFAASNVSNFEVAPTG
                     FNEVNTLNDVNIKTEINSFNSIHYTTCVGLTSGGTVAANQQNCVAFTPNAFTNSVSAS
                     GGSGGSIVYQWQKLVSCGSFSNISGATAATYASGSVTKTTIFKRKAWRNCSGGTGNVA
                     VYSNEVTVLIVYPGTISGDESGNCNFNSSTITGTNHSADNNYCLSDGTPQNQSYQWQK
                     SIDGGNTWTDISGANAQNYSPGSITQTAKFRRATTVSGYTTYSNVVTKTVNISPITLS
                     TESFPNSFNTGFTAPINTNFNGSTGSWGAYSTTARSTVVVHSAYYQSSPYALKMVNYS
                     TNGYAATSSKATSPKIDLSAGNNVELSFKLYTYSVSSANTCYSIKLEFSNDNGATWNL
                     IESSTAQEIANAYGQGTWNTVSLTVPGNYYNSNFRYRFATEQAAGCNYNTYLYIDDIT
                     FKSYPTCLPCPSSTNAGIDSTQCNNPDFTMAATPAPQGSTGTWSVINGSANISNVNSP
                     TSVITVTGSPATLRWTVSTPGCPDVYDDVVLTNTNNVTTLSYESFTNSFNTGFTAPIN
                     TTFTGSTGTWSAYSSTSRSTIVVNNAHVQSAPYALKIVNYNTNGYAATTSRAKSPKIN
                     LTGGNNVEMSFKLYTYSVSNSNTCFTFNVEISNDNGTTWTTVLAQTSKQLENTYGQGT
                     WNTITIPIPVSYYNANFRYRYSGVQQANCCYDTYLYIDDIRLNSNTPCVPCPSSTDAG
                     SDITQCNDANFTMTATPAPQGSTGTWSVVSGTATILNANSPTTTVTTTSSPVTLRWTL
                     VTPGCPNVTDDVVLTNNAQVVTLSNESFTNSFNTGFSAPINSTFTGAIGTWGAYSSTS
                     RSTIVVNNAHIQSAPYALKIYSVNTNGYSASTARATSPTVNLTSGSNVEMSFRLYTYA
                     VNANNTGYSFNVEFSSDNGSTWTNVFTRTSQELYNVYGQSTWNTINISVPNSYYNANF
                     KYRLSGVIKANTNYDTHLYIDDITFKSYPPCLPCPTEADAGADISNCNNATFTMDATP
                     APQGSTGTWSVISGSASISNVNSPTSSVTVSGSPATLRWTITTPGCPTIYDDVVLTNN
                     DNVVTLSTESFPNSFNTGFTAPINSTFTGSTGTWGAYSSTSRSTIVVHNSNYQSAPYA
                     LKLYNISTSGYAATTARATSPKLDLTGGDSVVMNFRILPHTVSGSNTCYTLKLEFSND
                     NGSTWNVMETGTAQDIYNTLGQGTWSTVSYVVPSMYYNANFKYRFVTSQAAGCNYNTY
                     IYVDDITFKSYPPCLPCPTNDDVDAGVDQGQCNNNIFTMSASAPPQGSTGTWSINSGT
                     ATIANPNSPTTTVTVSVSPVTLRWTLTTPNCSNVYDDV"
BASE COUNT         2291 a         1068 c         1073 g         2168 t
ORIGIN      
        1 cctgccacag agcagagtac agagaaagac gaccctgatg cagttgcaac ccataccaat
       61 aaaacaacta aagttaccaa acctgttgca gtagcacctg ttaataagcc taaaactgta
      121 atgccaaaat atagtggtgg taatagcaat ggtggtaata gtaaagataa ttataacgac
      181 agtagaagtg agggtaacga taaatcgggt gtgggagata aaggcaagcc ttggggaagt
      241 attgatggaa ctgtttatga tggaccaggt ggaacaagag tttatggttt aggtaaaaga
      301 agaataacac gctttcctac atttacagat gacttttcag aaaatgcaaa agttgctatt
      361 aatattgtgg tagataaaaa cggaaatgta acgtatgccg ctgtaaatcc taatggtaca
      421 agcactgcaa ataaacaaac tagagctgtt gcattgaaaa gagcaaaaga aattaaattt
      481 gaaacaagtg agcaagagca acgaggtact attattttag acatgagagt ttctgggtaa
      541 cttgctttaa ttaacttatg aactataaag agtctgttga ctatttattt gaacaactac
      601 ctatgtttag cagggtagga ggcatggcta taaaagaggg cttaaccaat actattttaa
      661 tctgtaacaa attaaacaat ccacaaaata aatttaagtc agtacatatt gcaggcacca
      721 atggcaaagg ctctgtaagc cacatgttag ctgcaatttt gcaaactgct ggattcaaaa
      781 caggcttata tacatcgcca catttaacag attttaggga gagaattaaa gttaatggta
      841 aaaaatgctc gcaagaattt gttgtgcaat ttactgagca aatgaagcct tttatagaag
      901 aatttaaacc ttcttttttt gaaattaccg taggcatggc ttttgaatat tttgcaaaag
      961 aaaaagtgga tattgccatt attgaaacag gtttaggtgg tagattagat agcacaaata
     1021 tcatcacacc aattttatcc atcattacta atattggatt agaccatgtt caaatgttgg
     1081 gcaattcatt accagaaatt gcagtagaaa aagcaggaat tattaagcaa aacgtatcag
     1141 taataattgg cgaaacaaat gcagcaacaa aaacagtttt tattaaatct gcacaggaaa
     1201 aaaatgcttc aatccagttt gcagatgaac tgtttaaagt aaataattat aagtacagca
     1261 ttgataactt aaccatagag attgaaaata ttaaacaaca cactaaagaa gtttttactt
     1321 tagatctaac tgggttgtat caacttaaaa atattatcac agtaattgca gctttaaaaa
     1381 aattacaaga tttagattgg aatatcaata atagccatat taaaactgca ttaaacaaaa
     1441 caaaacagct aacaggttta aaaggcagat ggcaagtaat cagaaaaaat aatccaccgg
     1501 tttttgccga tgttgctcat aattcagatg gtgtaaagca agtaatacaa catctacaat
     1561 tactgtcatt taatcaactt catttaattg taggtatggt taaagataaa gatgtaagtg
     1621 cagttttatc tttattacct tcaaatgcaa aatattactt tacacaagct caaattccta
     1681 gagcaatgcc tgcagaggat ttacaaaagt tggctgctaa atttaattta gaaggctact
     1741 cttttagcaa tgtaaattta gccatagaat atgcaataca gaaagcatct aaaagtgatt
     1801 tagtacttat aatgggtagt gtttttttaa ttggcgaaat aaattcaata taaccaataa
     1861 aaaatatcac cccaaaaggg taaagtttta aatttttatt tcttttacaa actagtacaa
     1921 aagcagtata attttatttt tgtagagctt taatagaaaa aagcattaaa gtcattaata
     1981 tccttataat cattcagttt tatttagggc ttaagcttat tttaatttaa ttattgtggt
     2041 tatttagaac atatttaaat tggcatcatt aacttattga ttgaatgtgc tttataacta
     2101 ttttttattt attttaatta atattaggta gaaactatat aaattttaag gataaaactt
     2161 aattctttta acttcaaaat tggctcaaaa tatttttttc aaaacctgaa tgttccaaaa
     2221 cttgctattt tcttctaact taaattttaa tgtttgaaat gtgaataaag tttccaataa
     2281 tgggacaaat cctcaaattg gacatatttt caaaatagat tgcaaccatt tcattttgaa
     2341 caacatcatt caaaaggttt gtataatttt gtattcaatt caattcacta tccattccta
     2401 tttaagagct gtttacataa atcctacaag ctaagttaaa ctcagttcct attaaaaatt
     2461 taactattca agtactctaa gaaacagaaa tgtttcatta agcaattgtg cttaaaaatt
     2521 ataaattatg aaattattaa acagacttac tttttttgtt ttattgtttg gctctttatt
     2581 atttaatgct tcaacagtat cagctcagtt gggctttgct gcttcaaacg tttctaactt
     2641 tgaggttgct ccaactgggt ttaatgaagt taatacacta aacgatgtaa atattaaaac
     2701 tgaaattaat tcttttaata gtatccatta tactacatgt gttggtctta caagtggtgg
     2761 aacagttgct gctaaccaac aaaattgtgt agcatttaca cctaatgcat ttaccaattc
     2821 tgtttctgca agtggtggtt ctggtggtag cattgtttac caatggcaaa agttggttag
     2881 ttgtggtagt ttttctaata tctctggtgc tactgcagca acttatgcat ctggttcggt
     2941 tacaaaaaca acaattttta aaagaaaagc ttggagaaac tgttcaggtg gtactggtaa
     3001 tgtagctgtt tattctaatg aagttactgt attaattgtt tatccaggaa ctatttcagg
     3061 tgatgagtct ggcaattgta attttaattc ctctactatt acaggtacca atcatagtgc
     3121 tgataacaat tattgtttaa gcgatggtac accacaaaac caaagttatc agtggcaaaa
     3181 aagtatagat ggtggaaata catggacaga tatttctgga gccaatgccc aaaactactc
     3241 accaggatct atcacacaaa ctgcaaaatt tagaagagcc actactgtta gtggttatac
     3301 tacttatagc aatgtagtaa ctaaaactgt aaatatttca cctattacac tttctacaga
     3361 aagttttcct aatagtttta atacaggctt tactgcacca attaatacta attttaatgg
     3421 ttctacaggt tcttggggtg catattcaac aacagctaga tcaacagttg ttgtgcatag
     3481 tgcttattac caatcttcgc cttatgcttt aaagatggtt aattacagca caaatggtta
     3541 cgctgctaca tcatctaaag ctacttctcc aaaaattgac ttatctgcag gaaataatgt
     3601 tgagttaagt tttaaattat atacttattc agtttctagt gctaatactt gttatagtat
     3661 taaactagaa ttttcaaacg ataatggagc aacttggaat ttaatagaaa gtagcactgc
     3721 tcaagaaatt gcaaatgctt atggtcaagg tacatggaat actgtttctt taactgtacc
     3781 tggtaattat tacaactcta attttagata tcgttttgca acagaacaag ctgctggttg
     3841 taattacaat acttacttgt atatagatga tattacattc aaatcttatc caacttgttt
     3901 gccatgccca tctagtacta atgcaggtat agattctaca caatgtaata acccagattt
     3961 tacaatggct gcaacgcctg caccacaagg atctacaggt acttggagtg tgattaatgg
     4021 cagtgcaaat atttctaatg ttaattctcc tacatctgta attactgtta caggttcacc
     4081 agcaacatta agatggacag tttccacacc tggttgccca gatgtttatg atgatgttgt
     4141 tttaactaat actaataatg ttactacatt atcttacgaa tcttttacaa acagttttaa
     4201 tacaggcttt actgctccta taaataccac atttactgga tcaactggta cttggagtgc
     4261 atattctagc acaagcagat ctacaattgt tgttaataat gcacatgtgc agtctgcacc
     4321 ttatgcctta aaaattgtaa actacaatac aaacggttat gctgcaacaa cttcaagagc
     4381 aaaatctcca aaaataaatt taacaggcgg taataatgta gaaatgagtt ttaagcttta
     4441 tacttactca gtttctaaca gtaatacttg ttttactttt aatgtagaga tttctaatga
     4501 taatggcaca acttggacta cagttttagc tcaaacttct aaacaattag agaataccta
     4561 tggtcaagga acatggaata caattactat tccaattcct gtaagttatt ataatgcaaa
     4621 cttcagatat agatatagtg gtgttcaaca agcaaattgt tgttatgata cttatttata
     4681 tattgatgac attagactaa attcaaatac accttgtgta ccttgccctt caagtacaga
     4741 tgctggttcg gatattacac aatgtaatga tgctaatttt accatgactg caacacctgc
     4801 accacaaggt tctactggta cttggagtgt tgtaagtggt acagcaacta ttttaaatgc
     4861 aaattcacca acaacaactg taacaacaac ctcttctcct gtaactttaa gatggacttt
     4921 agttactcct ggttgtccta atgttactga tgatgtagtt ttaaccaaca atgctcaggt
     4981 cgttacttta tctaacgaat cttttacaaa tagttttaat acaggatttt cagctccaat
     5041 taactcaaca tttacaggtg caattggtac atggggtgct tactcttcaa caagtagatc
     5101 caccatagtt gttaataatg cacatataca atctgcacct tatgcattaa agatttatag
     5161 tgtaaataca aatggttata gtgcaagcac agcaagggca acatcaccta cagttaactt
     5221 aactagtggc tctaatgtgg aaatgagttt tagattatac acttatgctg taaatgctaa
     5281 taatacaggc tactctttta acgtagaatt ttcgagtgat aatggtagta catggactaa
     5341 tgtatttaca agaacttctc aagagttata caatgtgtat ggtcaaagta cttggaatac
     5401 tatcaacatt tctgttccta atagctacta taatgcaaac tttaaatatc gtttaagtgg
     5461 tgtaataaaa gctaatacaa attatgacac acatttatac atagatgata ttacttttaa
     5521 atcataccca ccttgtttgc cttgcccaac agaagctgat gcaggagctg atattagtaa
     5581 ttgtaataac gcaacattta caatggatgc aacacctgca ccacaaggat caactggtac
     5641 atggagtgtg ataagtggaa gtgcatcgat ttcaaatgta aattctccta caagttcagt
     5701 aactgtttca gggtctcctg caactttaag atggacaatt acaacaccag gctgccctac
     5761 tatttatgat gatgttgtgt taactaataa tgataatgtt gttacattat caacggaatc
     5821 tttccctaac agttttaata ctggctttac ggcaccaatt aattctacat ttacaggttc
     5881 tacaggtaca tggggtgcct actcaagtac atcaagatct acaatagttg ttcataattc
     5941 aaattatcag tctgctcctt atgctttaaa gttgtataat attagcacaa gtggatatgc
     6001 agcaactaca gctagagcaa cctctcctaa attagattta acaggtggtg atagtgttgt
     6061 aatgaatttt agaatattac ctcacacagt ttctggctct aacacttgtt atacattaaa
     6121 attagaattt tctaatgata atggtagtac atggaatgta atggaaacag gtacagcaca
     6181 agatatttat aataccttag gtcaaggaac ttggtcaaca gttagctatg tagttccatc
     6241 tatgtactat aatgctaatt ttaaatatcg ttttgttact tcacaggctg ctggttgtaa
     6301 ttataatact tatatttatg tagatgatat tacatttaaa tcataccctc catgtttacc
     6361 ttgtcctacc aacgatgatg tagatgcagg tgtagatcaa gggcaatgta ataataatat
     6421 ctttacaatg tctgcttctg cacctccaca aggttcaaca ggaacttgga gcattaatag
     6481 tggcactgca actattgcaa atccaaattc tccaacaaca accgtaacag tatcagtttc
     6541 tcctgtaaca ttaagatgga cattaaccac tcctaactgt tctaatgtat atgacgatgt
//
LOCUS       DAOQHP010000081         6556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6313_length_6556_cov_3.937548, whole genome shotgun sequence.
ACCESSION   DAOQHP010000081 DAOQHP010000000
VERSION     DAOQHP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_6556_cov_3.937548"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLY81_03895"
     CDS             <1..253
                     /locus_tag="PLY81_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNF29399.1"
                     /translation="IMSSITAETTQVMENIKAILHQAEIDFSHVVKTTIFLSDMSLFT
                     EVNEVYAKYFLTDYPARETVTVKGLPKDANVEISVIAHR"
     gene            278..3643
                     /locus_tag="PLY81_03900"
     CDS             278..3643
                     /locus_tag="PLY81_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF29400.1"
                     /translation="MSYTPKNKVRIVTAASLFDGHDAAINIMRRIMQSKGAEIIHLGH
                     NRSVKEIVECAIEEDAHGIAITSYQGGHVEFFKYMKDLLEENSCGHIKIFGGGGGTIL
                     PDEVRELHDYGITKIYTPDDGRHMGLEGMIEDVINTLKLTSLITHNWNGKLPLDEVKD
                     VRKVARAITLAENGNDYKDLVKQLATANKHQAAVLGITGTGGAGKSSVTDEIVRRFLH
                     TFTDKTIAVISVDPSKKKTGGALLGDRIRMNAISHPRAYMRSLATRDDNTALSAYVKD
                     AIEICKKANYDFIILESAGVGQSDASILDYCDISMYVMTPEYGAASQLEKINMLDYAD
                     VVCINKFDKAGALDAYQDVCKQYKRNHKLFTAKNEDVPVIGTIASKFNDEGVNHLFDK
                     LLQVIETKTNIRFGIFTHQATAKVTDAVIPAKRVRYLSEISSSIRDYNQLAIDQCLLA
                     TKLYKLQGSLEILKTTTDTALLEQLQQQINFYTEQLTPVAKKLIHNWEEKKTAYSKDF
                     YEYKVRDKVIQQEMFTTSLSGTRIPKVVLPKYKDWGDLLKWQCQENFPGSFPFTSGVF
                     PLKREGEDPTRMFAGEGGPERTNKRFHYVSIGQPAHRLSTAFDSVTLYGEDPTHRPDI
                     YGKIGNSGVSIATVDDAKKLYSGFDLCHPKTSVSMTINGPAPIILAFFMNAAIDQECE
                     KYIEQNNLWATVEKISKEKYITSNAPLYYNPSSPERLPEGNNGLGLKLLGLSGEEVLP
                     KEIYEQLKAKALQSVRGTVQADILKEDQAQNTCIFSTEFALKLMGDVQEYFIQQNVRN
                     FYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMNIDDFAPNLSFFFSNGM
                     DAEYSVIGRVARRIWAKAMKYKYKANKRSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGSAKTENFIQGSFAI
                     EELTDLVEEAVLTVFDKITERGGVLGAMERMYQRNKIQEESMHYEMQKHTGELPLIGV
                     NTFLNKKGSPTITPTEVIRSTTEEKEQQIQNLQAFHKRNEHKSQQELQQLKAAAVNNQ
                     NLFDVLMQTVKYCSLGQITSALYEVGGQYRRNM"
     gene            3676..4845
                     /locus_tag="PLY81_03905"
     CDS             3676..4845
                     /locus_tag="PLY81_03905"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNF29401.1"
                     /translation="MSSNNATNKKFKPATIYIHAGMEPDPSTGSVMVPIYQTSTFVQE
                     SPGNHKGYEYARSQNPTRFALEEAFAKIENGNHALAFSSGVAATDAVIKLLQPGDEVI
                     AANDMYGGTYRLFTKVFEKFGIIFTFIDTTNADNVAKAISAKTKLVWVETPTNPLMNI
                     TDIAAIAAITKANNCLLCVDNTFASPYLQNPLNLGADIVMHSATKYLSGHSDVIQGCL
                     VMNDKHLRDDLYFIQKSCGAVPGPLDCFLVLRGIKTLHLRMQRHCENGEKIAHWLRKH
                     PKVKQVYWPGFEDNKNYSIAKSQMNGFGGMISFELVNESVEEAKRVLSSTHLFSLAES
                     LGGVESLINHPASMTHASIPREERIKNGLSDGLIRLSVGIEDADDLINDLDKAIG"
     gene            complement(4854..>6556)
                     /locus_tag="PLY81_03910"
     CDS             complement(4854..>6556)
                     /locus_tag="PLY81_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNF29402.1"
                     /translation="AICTFLLMFFFGADYGHFSYVSTRLNASALNFAEDAKISFEMLW
                     ESYPIFWMILALIILAFLFYKLYTLTHQRIEANNITSINYLRRWYLVISLFLIWGIYG
                     SINSKPLKWKDAFIYKNNFKSYLALNPLQNFFTTLRFRKPVADGNLVHKYYNDIANFL
                     QFDSLTKQQASFKRWIYPHSHALESKPNVVLVICESFSMYKSSMSNNPLNATPYFNTL
                     SKQGIFFNRCFTPHFATARGIFATLTGIPDVQLSKFSSRNEESLSQHTIINNFDDYNK
                     FYFIGGSSEFNNYKGLLKNIKNLQLFEEGSYTSPKVNVWGISDKDLFTESNNILAKQE
                     KPFFAIIQTAGNHRPYTIPENDKEFKRKIIDKDTLQKYGFLSEEEYNAFAYMDYSIEH
                     FIKTATKEKYFQNTIFVFVGDHGVEGNASAMYPNAWTINRLSDEHVPLLFFAPHLLQP
                     KQYQKVVSQIDVLPTIAGLVYQPYFNTTLGRDILKPGIKNNGAFIIHHDEGKIGWLTD
                     SFYFVKNIRFEQEELIPIQSNIANWSPTQETEIKKKYSVFTSAFYETAKWMLINNKQK
                     "
BASE COUNT         2401 a          985 c         1155 g         2015 t
ORIGIN      
        1 aattatgtca agcattacag cagaaacaac gcaagtaatg gaaaatataa aagcgatttt
       61 acatcaagca gaaattgatt tcagccatgt agtaaaaaca actatttttt taagtgatat
      121 gtcgcttttt acagaagtta atgaagtata tgcaaaatat ttcttaaccg attatcctgc
      181 tcgagaaaca gtgactgtaa aaggcttacc taaagatgct aatgttgaaa ttagtgtaat
      241 tgcacataga tagaatatat agataaatct tttcattatg agttatactc ccaaaaacaa
      301 agttcgtatt gtaactgctg caagtttatt tgatggacat gatgctgcaa taaatattat
      361 gcgtagaata atgcaaagca aaggggcaga aattattcat ttagggcata acagaagtgt
      421 aaaagaaatt gtagaatgtg ctattgagga agatgctcat ggtattgcta tcacatcgta
      481 ccaaggtggg catgtagagt tttttaaata catgaaagat ttgttagagg aaaatagttg
      541 tgggcatatc aaaatttttg gtggtggtgg cggaaccatt ttaccagatg aagtaagaga
      601 attgcatgat tatggaatta caaaaattta tacccccgac gatggcagac acatgggcct
      661 tgaaggaatg attgaagacg taattaacac cttaaaacta accagcttaa ttacacataa
      721 ttggaatgga aagctacctt tagacgaagt gaaagatgta agaaaagttg ctcgagctat
      781 tacacttgca gagaacggta atgattataa agatttggta aaacaacttg ctacagcaaa
      841 taaacatcaa gcagcagttt taggaattac aggtacaggt ggtgcaggaa aaagctcagt
      901 aaccgatgaa atagttagac gatttttaca cacttttacc gataaaacca ttgccgttat
      961 ttcggttgac cccagcaaga aaaaaacagg tggtgcatta ttaggcgaca gaattagaat
     1021 gaatgcaata tcacatccaa gagcttatat gcgtagcctt gctaccagag atgacaatac
     1081 tgctttaagt gcttatgtaa aagatgctat agaaatttgc aaaaaggcca actacgattt
     1141 tattatttta gaaagtgcag gtgtaggaca aagcgatgca agtattttag attattgtga
     1201 tataagcatg tatgtaatga ccccagaata tggtgcagca tcgcaactag aaaaaataaa
     1261 catgctagat tatgccgatg tagtttgtat taataaattt gacaaagctg gtgctttaga
     1321 tgcatatcaa gatgtatgta aacaatacaa aagaaaccat aagcttttta ctgcaaaaaa
     1381 tgaagatgtg cctgttatag gcaccatagc aagtaagttt aacgacgagg gtgtaaacca
     1441 tctttttgat aaattattac aagtaataga aaccaaaacc aacataaggt ttggcatatt
     1501 cacccatcaa gcaacagcaa aagttaccga tgctgtaata ccagcaaaaa gagtgagata
     1561 tttaagtgaa atatcctcct ctattagaga ctataaccaa ttagctatag accaatgctt
     1621 gcttgcaact aaattatata aactacaggg cagtttagaa attttaaaaa ccaccaccga
     1681 tactgctttg ctagaacagt tacaacaaca aataaatttt tataccgaac agcttacacc
     1741 tgtagcaaaa aaattaattc ataattggga ggaaaagaaa acagcttata gtaaagattt
     1801 ttatgaatat aaagtaagag ataaagttat tcagcaagaa atgtttacaa ctagcttatc
     1861 cggcacaaga atacctaaag ttgttttacc aaaatataaa gattggggag acttattaaa
     1921 atggcaatgt caggaaaatt ttcctggaag ttttccattt acatcgggtg tatttccttt
     1981 aaaaagagaa ggtgaagacc ctactagaat gtttgcagga gaaggcggac ctgaaagaac
     2041 caataaacgt tttcattatg taagtattgg gcagccagca catcgtttaa gtactgcttt
     2101 tgatagtgtt actttatacg gagaagaccc aactcatcgc ccagatattt atggaaaaat
     2161 tggtaacagt ggcgtaagca ttgctactgt agatgatgct aaaaaattat acagcggttt
     2221 tgatttatgt catcctaaaa cttctgtatc aatgaccatt aatggtccag ctcctatcat
     2281 tcttgcattt ttcatgaatg ctgcaattga tcaggaatgt gagaaatata tagaacaaaa
     2341 taatttatgg gcaaccgttg aaaaaattag taaagaaaaa tacattacct caaatgcacc
     2401 tctttattac aacccatcat cgccagaacg tttgccagaa ggcaataatg gtttaggttt
     2461 aaaattatta ggtttaagcg gagaagaggt attgcctaaa gaaatatatg aacaacttaa
     2521 agcaaaagct ttacaaagtg ttcgtggtac agtacaggca gatattttaa aagaagacca
     2581 ggctcaaaat acttgtattt tttctacaga gtttgcatta aaattaatgg gagatgtaca
     2641 agaatatttt atacaacaaa atgtaagaaa tttttatagt gtttctatca gtggttacca
     2701 tattgccgaa gctggtgcaa accctattac acaattagct tttacattgg caaatggttt
     2761 tacctatgta gaatattatt taagcagagg aatgaatata gacgattttg cacctaacct
     2821 ttctttcttc ttcagcaatg gtatggatgc agaatacagt gtaattggca gggttgcaag
     2881 acgtatttgg gctaaagcaa tgaagtacaa atacaaagca aataaacgca gccaaatgtt
     2941 gaaatatcat attcaaacaa gtggtagaag tttgcatgct caagaaatag attttaacga
     3001 tataagaacc accttgcaag cattgtatgc tatttatgat aattgcaatt cattacacac
     3061 taatgcttat gatgaggcaa ttactacacc aacagaagaa agtgtaagaa gagcaatggc
     3121 tatacagtta attattaata aagaattagg cagtgctaaa acagaaaatt ttattcaggg
     3181 tagttttgca atagaagaat taaccgattt ggtggaagaa gctgtactta ctgtatttga
     3241 taaaattaca gaacgaggtg gcgttttagg agcaatggaa agaatgtatc aaagaaataa
     3301 aatacaagaa gaaagtatgc actacgaaat gcaaaaacat acaggagaac ttccattaat
     3361 tggggtaaat accttcttaa ataaaaaagg tagcccaacc attactccta cagaagtaat
     3421 tcgtagtacc acagaagaaa aagaacaaca aattcaaaac ctacaggcat ttcataaacg
     3481 taatgagcat aaaagccaac aagaattgca acaattaaaa gcagcagcag taaacaatca
     3541 aaatttattt gatgtgttaa tgcaaactgt aaaatattgc agcttggggc aaataacaag
     3601 tgctttgtac gaagtagggg gccaatacag aagaaatatg tagttaatat aattaattaa
     3661 aaaaaataaa aaattatgag ttcaaataac gcaaccaata aaaaatttaa accagcaaca
     3721 atatatatcc atgctggcat ggaacctgat ccaagtactg gctcagtaat ggtgcctata
     3781 tatcaaacaa gtacatttgt tcaagaatct ccaggtaacc ataaaggtta cgaatatgcc
     3841 agaagccaaa accctacacg ttttgcatta gaagaggcat ttgctaaaat agaaaatggt
     3901 aaccatgctt tggcatttag tagtggcgtt gcagcaacag atgcagttat aaaacttttg
     3961 cagccaggcg atgaagttat tgctgcaaat gatatgtatg gtggcactta cagattattt
     4021 acaaaagtgt tcgaaaaatt tggaatcatt tttactttta ttgatactac taatgcagat
     4081 aatgtagcta aagcaatttc agcaaaaacc aaactggttt gggtagaaac gccaactaac
     4141 ccattaatga acattacaga tattgcagca atagcagcta ttactaaagc caataattgc
     4201 ttgctttgtg tagataatac ttttgcatca ccgtacttac aaaatccttt aaacttagga
     4261 gcagatattg taatgcacag tgctacaaaa tatttaagtg gacatagcga tgttatacaa
     4321 ggctgccttg taatgaatga taaacatctg agagatgatt tgtattttat acaaaaaagt
     4381 tgtggtgcag ttcctggtcc tttagattgt tttttggttt tacgtggtat taaaactttg
     4441 catttacgca tgcaacgcca ttgtgaaaat ggagaaaaaa ttgctcattg gttacgcaag
     4501 catccaaagg ttaaacaagt ttattggcca ggatttgaag ataataaaaa ctatagcatt
     4561 gctaaaagtc aaatgaatgg ttttggtgga atgataagtt ttgaattagt aaacgaaagt
     4621 gtagaagagg ctaaaagagt tttatccagc acacaccttt tttcattagc agaaagttta
     4681 ggaggtgtag aaagtttaat taaccatcct gcaagtatga cacatgcaag tatcccaaga
     4741 gaagaaagaa ttaaaaatgg tttatctgat ggattaatta gattaagtgt tggtattgaa
     4801 gatgcagatg atttaattaa cgatttagat aaagcaattg gatagttaat aaattatttc
     4861 tgtttattat taatcagcat ccattttgct gtttcgtaaa aagcagatgt aaaaacagaa
     4921 tatttctttt ttatctctgt ttcttgtgtg ggtgaccagt ttgcaatatt agattgaata
     4981 ggaattaatt cttcttgttc aaatcttatg ttttttacaa agtagaaaga gtctgttagc
     5041 cagccaattt ttccttcatc gtgatgtata ataaatgctc cattgttttt tatacctggt
     5101 tttaagatat ctctgcctaa tgttgtatta aaataaggtt gatatactaa acctgcaata
     5161 gttggtaata catctatttg tgaaacaact ttttgatatt gtttaggctg taacaaatgt
     5221 ggtgcaaaaa ataatagagg tacatgctca tcgctaagcc tgtttattgt ccatgcattt
     5281 ggatacattg cagaagcatt accttccact ccatgatctc ctacaaaaac aaaaatagta
     5341 ttttgaaaat atttttcctt agtggcagtt ttaataaaat gttctattga ataatccata
     5401 taagcaaaag cattgtattc ctcttccgat aaaaatccat acttctgaag tgtatcttta
     5461 tcaataattt ttcttttaaa ttctttatca ttttctggaa tggtataagg cctgtggttg
     5521 ccagcagttt gaatgatagc aaaaaatggc ttttcttgct ttgctaaaat gttgttactt
     5581 tcagtaaata aatctttatc gctaataccc catacattta cttttggcga ggtataactt
     5641 ccttcttcaa acaattgtaa attttttata ttttttaata accctttgta gttattaaat
     5701 tcactactgc ctccaataaa ataaaattta ttatagtcat caaaattatt aataattgta
     5761 tgctggctta ggctctcttc atttctacta ctaaactttg ataattgtac atctggtatg
     5821 cctgtaagtg ttgcaaaaat gccacgtgct gttgcaaaat gtggtgtaaa acatctatta
     5881 aaaaaaatac cttgtttgct taaagtgtta aaataaggtg ttgcatttag cggattatta
     5941 ctcatgctac ttttatacat actaaaactt tcgcaaataa ccaacaccac atttggtttg
     6001 ctttctaatg catggctatg tggatatatc caccttttaa aactagcttg ctgttttgtt
     6061 aagctatcaa attgtaaaaa attagctata tcgttataat atttatgaac aagattacca
     6121 tcggctacgg gttttctaaa acgaagtgta gtaaaaaaat tttgtaaagg gtttagtgct
     6181 aagtacgact taaaattatt tttatatata aaagcatctt tccactttag aggcttgctg
     6241 ttaatgctac cataaatgcc ccatattaaa aaaagtgaaa taaccaaata ccatcttctt
     6301 aagtagttta tgcttgttat attatttgcc tcaatacgtt ggtgtgttaa agtatataat
     6361 ttataaaaca aaaaagccaa tataattaaa gccaaaatca tccaaaaaat tggatacgac
     6421 tcccaaagca tttcaaaaga aatttttgca tcttcggcaa agtttaatgc acttgcattt
     6481 agccttgtac taacataaga aaaatgtcca taatctgcac caaagaaaaa cattagtaaa
     6541 aaagtacaaa ttgcca
//
LOCUS       DAOQHP010000082         6489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6417_length_6489_cov_4.024868, whole genome shotgun sequence.
ACCESSION   DAOQHP010000082 DAOQHP010000000
VERSION     DAOQHP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_6489_cov_4.024868"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLY81_03915"
     CDS             <1..449
                     /locus_tag="PLY81_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29403.1"
                     /translation="IIHKKNIFATYLFLIMKYSQLIGILAAIAIIGVCYMPWVYVPSI
                     NVTLSGINGKVNEQLTFGRQVIPHTALSLLLILFFLIPKIWAKRTNLFIGFISLSWAI
                     KNYIIFSMCRQTICPDKKAGLYLLIILAFIVQISTLLPKLTVKQKD"
     gene            580..2637
                     /locus_tag="PLY81_03920"
     CDS             580..2637
                     /locus_tag="PLY81_03920"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNF29404.1"
                     /translation="MKKIYPGLLAVVLFYTLTANAQVNYTYNAYQSTYVHLEDGITPA
                     LDNPTPVGYYEDDEGFANQIPIGFTFNFNNTNYTHLNINVNGFVTFGSNFTMNVGSRY
                     STNNLMNGPMQENIRPIIAPLWDDLRLKNNKCLSYAVKGNAPNRIFIVQWDSASWNYN
                     LLDPAISFQMILHEGTNKVQFIYKALEGKLSDASASVGIATCSSCTGSFLSVTSIDKN
                     AQISGLEENKYINSLPTTGTLIEFEPGKNELPSNVMVNAYNTHEITLSWNTSAIAEYD
                     YAITTSPLQPLTFSTTNTPSATLQNLEKGKQYYLHVRTSTAAGKSGWVSIPAKTASYT
                     TLPYVEKFEGISIPNNPEAIHIANPLGGNSWQTIAVPGVPAFNYAISYKGDNINNADA
                     WFMLPAMQLTGGLTYRLQFKYRASDSLGGDQKLEVKIGKMMNNGMIGWQTIYKNVKIN
                     QLKFQDISKLFAAPTSDEYFIAFRCFSEKSNTAIWIDDIDVSKVKPLPIKMIAFSGFR
                     EGFVNKISWQTSAEFNNSSFELQRSSDGKNFTSINKIETKANNGTSSTTLNYSLIDNT
                     PNTVDYYRLKVTDKESNDFYVQDIIKISSELPFLISAQKTYPNPATNVLNTVVYSKYK
                     TVGIIKIMDNYGHIVKAYSINIEAGDNIIQTDISQFNRGMYFVKVENKIGKAGNAKSF
                     IKQ"
     gene            2793..4061
                     /locus_tag="PLY81_03925"
     CDS             2793..4061
                     /locus_tag="PLY81_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29405.1"
                     /translation="MKNLIICLVCFLYIPFANAQINYSFTTSGASFDMVTNGTTPMFT
                     GNGTDPLADEGFVNYIPIGFSFSYNNYSNTYNEVAISSNGFISLTELSDSYVINNLNN
                     GASGERPIIAPLWDDLDLSLTSNLTYTTTGSAPNRVFTVQWLNVNWGFGSTSAAISFQ
                     LKLYETSNWIEFIYRPEAGFATSPSASIGLTALGTGTNNFVSIGGFSLTPSVSVSSES
                     NNITAKPNNVIKYTFKPGVLPVKMEYFTVTKDKEKHILKWQTVQETNHKQFNIQRSNN
                     GVNFSNIASQPSFESNGNSKTYLYADTKPLIGNNYYRLQQIDVDGKTNYSSIVYIKNV
                     NNNWASLNIYPNPVVNNLQVNINTHKAEKVNISVTDISNTVLLHSVYYTQPSENLYIL
                     NVQNLAKGTYFIKVYNSSTEQFIVQKFIKN"
     gene            complement(4058..5059)
                     /locus_tag="PLY81_03930"
     CDS             complement(4058..5059)
                     /locus_tag="PLY81_03930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNF29406.1"
                     /translation="MKFRYEFNIKSLPKAINHNHKIFLIGSCFTENIGEKLKKYKFSV
                     LENPNGILFNPVSVSEALINIIENKQYVANDLFEYNEAWHSWQHHSRFSGITVEETLE
                     KINQSTATAHQYLKQADYLLITLGSAWIYTLTNKANNATVGTVAANNHKAPADWFERK
                     LPDVAQVITVLVTMLNKLGSINPNIQVIFTISPVRHLREGAIENNRSKAALISAVHYL
                     IDNLPKLYYFPAYELVIDDLRDYRFYAEDLVHPNYAATDYVWSKFVQSCMQKETIELM
                     EIIKEINLAFQHKPFNAHSNQHTQFLATYFAKTKALTKKYANLDFDKELAYFKNLND"
     gene            5208..5636
                     /locus_tag="PLY81_03935"
     CDS             5208..5636
                     /locus_tag="PLY81_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29407.1"
                     /translation="MKKQIIGIVLIVAMVSFSGCKKAIEKIKEQEIVNAITNGKWVVS
                     KFVEGTTTTTTDFAGWETIYYENGTFTSSKTGETNQTGTWAGNASNWTFTITFNATPP
                     TPLNKLGGVWTVTRAVSTDKGSYAKTEAGVNYELEMTKMP"
     gene            complement(5710..6372)
                     /locus_tag="PLY81_03940"
     CDS             complement(5710..6372)
                     /locus_tag="PLY81_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29408.1"
                     /translation="MSNFLLSAKNITKKYNELPILKGIDIAIESNQIVSIVGSSGAGK
                     STLLHILGTLDKADSGEIWLNDIRVDLLSGKKLAAFRNMHIGFIFQFHHLLPEFSAIE
                     NVYIPAWIAKRKTTEVKKEAAQLLDILGLSNRLNHKPNQLSGGEQQRVAVARALINKP
                     SIVFADEPTGNLDSKNAKELHHLFTQLCTNLHQTFLIVTHNEELASLSNRVLHMKDGY
                     MV"
BASE COUNT         2355 a          968 c          944 g         2222 t
ORIGIN      
        1 ttataattca caaaaaaaat atatttgcga cctatttatt tttaattatg aaatattctc
       61 agttaatagg catattagca gctatagcaa taattggtgt ttgttatatg ccttgggtat
      121 atgttcctag tattaatgta actttatctg gaataaatgg taaggtgaat gagcagttaa
      181 cttttggccg ccaagtaatt cctcatacag cattaagttt attgttaatt ttattctttt
      241 taattccaaa aatttgggct aaaagaacca atttatttat aggttttata agtttaagtt
      301 gggcaataaa aaattatatc attttttcga tgtgtaggca aacaatatgc cctgataaaa
      361 aagctggttt atacctatta ataattttag cttttattgt tcaaatttct acactattgc
      421 caaaacttac agtaaaacaa aaagattaaa gtaagaagaa atctaccttg tgcacattgt
      481 gtaaataatt gtattcatat ttttttgctt ctaacacaat aaaataatta attattagtt
      541 tattagttag caaatatgct ttaatccaat atcaatgcta tgaagaagat ttacccaggt
      601 cttttagctg tagttttatt ttatacctta actgcaaatg cacaagtaaa ctatacttac
      661 aatgcttatc aaagtactta tgtgcattta gaagatggta ttacaccagc tttagataat
      721 ccaacacctg taggttatta cgaagatgat gaaggttttg ctaatcaaat acctattggc
      781 tttactttta attttaataa tacaaactat acacatttaa atataaatgt aaatgggttt
      841 gtaacatttg ggtcaaactt tactatgaat gttggtagcc gttacagcac aaacaattta
      901 atgaatggcc caatgcaaga aaatatcagg ccaattattg cccctctttg ggatgattta
      961 agactaaaaa acaataaatg tttaagctat gctgtaaaag gaaatgcacc taacagaatt
     1021 tttattgtac aatgggatag tgcatcttgg aattataatt tattagatcc tgcaatatcc
     1081 tttcaaatga ttttacatga aggaactaat aaggttcaat ttatttataa agcattagaa
     1141 ggaaaactat cagatgctag tgcttctgta ggtattgcaa cttgtagttc ttgcacagga
     1201 agttttttat cggtaacatc aattgataaa aatgcacaaa taagtggatt agaagaaaac
     1261 aaatacataa attctttacc tacaacaggc actttaatag agtttgaacc aggtaaaaac
     1321 gaattaccat ctaatgtaat ggtaaatgct tataatacac atgaaataac tttatcttgg
     1381 aatacttctg caatagcaga atatgattat gcaattacta cttctccttt acagccttta
     1441 actttttcta caactaatac gccttctgca acacttcaaa acttagagaa aggaaagcaa
     1501 tattatttac atgtaagaac aagcactgca gcaggtaaaa gtggatgggt ttcaatacct
     1561 gctaaaactg catcttatac taccctacct tatgtagaaa agtttgaggg tatttctatt
     1621 cctaataatc ctgaagcaat tcatattgcc aatccattag gtggcaattc ttggcaaaca
     1681 attgctgttc caggtgtgcc tgcttttaat tatgccatca gttataaagg agataatatt
     1741 aataatgccg acgcatggtt tatgttgcct gctatgcaat taacaggcgg attaacttat
     1801 cgtttacaat tcaaatatag agctagcgat tccttaggtg gagatcaaaa attagaagta
     1861 aaaattggaa aaatgatgaa taatggaatg ataggttggc aaactattta taaaaatgta
     1921 aaaattaatc aactaaaatt tcaggatatt tctaaacttt ttgcagcacc taccagcgat
     1981 gagtatttta tagcttttag atgtttttct gaaaagagta acactgccat ttggattgat
     2041 gatattgacg taagtaaagt aaagccttta ccaataaaaa tgatagcttt tagtggcttt
     2101 agagaaggtt ttgtaaataa aatttcttgg caaacctcag cagagtttaa caatagcagc
     2161 tttgaattac aaagaagttc cgatggtaaa aattttacca gcataaacaa aatagaaacc
     2221 aaagcaaata acggaacaag ttcaacaaca ttaaactata gcttaataga taatacacca
     2281 aacacagttg attattatag gttgaaagtt actgataaag aaagtaatga tttttatgtg
     2341 caagatatta ttaaaataag tagcgaatta ccctttttaa ttagtgcaca aaaaacttat
     2401 cctaaccctg ccacaaatgt tttaaatact gttgtttact caaaatataa aacagtgggt
     2461 ataataaaaa ttatggataa ttatggccat attgtaaaag cttatagtat taatattgaa
     2521 gcaggagata acattattca aacagatatt agccaattta ataggggaat gtattttgtg
     2581 aaagttgaaa ataaaattgg caaagcaggc aatgcaaaat cttttataaa acaataaaat
     2641 ctttaggagc cacttgaaag tatcgctaaa tgcatctacc tgatgaaaaa ggcaagaagc
     2701 aaaaagctca actcttttca ataaatgttt tgatagctca tttattgctg ttgttttctt
     2761 gtggcttcta aaactaatta actataacca atatgaaaaa cctaattatc tgcttagtat
     2821 gctttttata catacctttt gctaatgctc aaattaatta ttcctttaca acttctggtg
     2881 ctagttttga tatggtaact aatggaacaa caccaatgtt tactggtaat ggaactgacc
     2941 ctttggctga tgaaggattt gtaaattata ttccaattgg tttttcattt agctataata
     3001 attatagcaa tacttataac gaagtagcta tttcatcaaa cggttttatt agccttactg
     3061 aattaagtga tagctatgta attaacaatt taaataatgg tgcaagtggc gaaagaccaa
     3121 taatagcacc actttgggat gatttagatt tatcactcac ctctaattta acttatacca
     3181 caacaggctc tgcacctaac agagtattta ctgttcaatg gctaaacgtt aactggggtt
     3241 ttggttcaac ttctgcagcc atttcttttc aactaaaatt atacgaaaca agtaattgga
     3301 ttgaatttat ttaccgccca gaagctggtt ttgctacttc cccatctgca tctattggat
     3361 taactgcttt aggaaccgga actaataatt ttgtttctat tggtggtttt tctttaacac
     3421 catctgttag tgtaagcagc gaatcaaaca acattaccgc aaagccaaac aatgtaatta
     3481 aatacacatt taagcctggt gtattaccag ttaaaatgga atatttcact gttactaaag
     3541 ataaagaaaa gcatatttta aaatggcaaa ctgtgcaaga aacaaatcat aaacaattta
     3601 atattcaacg ttctaataat ggtgtaaact ttagtaacat agcatcacaa ccaagctttg
     3661 aaagtaatgg aaatagtaaa acctatttat atgcagatac taaaccatta ataggtaaca
     3721 attattacag acttcaacaa atagacgtag atggaaaaac aaattactct tctattgtgt
     3781 atataaaaaa tgtaaacaat aactgggctt cattaaatat ttatcctaac ccagttgtaa
     3841 ataacttaca agtaaatatt aacacacata aagcagaaaa ggtaaatatt agcgttacag
     3901 atatttctaa cacagtttta ttacactccg tttattatac acagccatca gaaaacttat
     3961 acattttgaa tgttcaaaat ttggctaaag gaacctattt tattaaagtt tacaattcat
     4021 ccacagaaca attcattgtt caaaaattta taaaaaatta atcgtttaaa tttttaaaat
     4081 atgctaactc tttgtcaaaa tcaaggttgg catatttttt tgttaatgct tttgttttag
     4141 caaagtaagt agcaagaaat tgtgtatgct gattagaatg tgcattaaat ggcttatgct
     4201 gaaatgccaa attaatttct ttaattattt ccattaattc tatggtttct ttctgcatac
     4261 aggattgtac aaatttgctc catacataat cagtagcagc ataattagga tgtactaaat
     4321 cttctgcata aaacctataa tctcttaaat catcaattac taattcatac gcaggaaagt
     4381 aatatagctt tggcaaatta tcaattaagt aatgtacagc agagataagt gctgccttgc
     4441 ttctattatt ttcaattgct ccttctctta aatgccttac tgggctaata gtaaatatta
     4501 cctgaatatt tggatttata cttcctaact tatttaacat agtaacaagt actgtaataa
     4561 cttgtgcaac atctggcaat tttctttcaa accaatcggc aggggcttta tgattatttg
     4621 ctgcaactgt accaacagtt gcattatttg ctttgttggt taaagtataa atccatgcac
     4681 tacctaaagt aattagcaaa taatctgcct gctttaaata ttggtgtgca gtagctgtag
     4741 attggtttat tttctctaat gtttcttcta cagttatacc agagaatctg ctatggtgtt
     4801 gccaactatg ccatgcctcg ttatattcaa acaaatcatt ggcaacatat tgtttattct
     4861 caataatatt aattaatgcc tctgaaacac ttacagggtt aaataaaatg ccatttggat
     4921 tttctaatac ggagaattta tattttttca acttttcccc tatattttca gtaaaacaag
     4981 agccaattaa aaaaatttta tggttatggt taattgcttt aggcagcgat ttaatattaa
     5041 attcatatct aaatttcata tagcaaatat attatgtagt gttgcctatt agcaataaat
     5101 taaacttaat ttcgttttat taccatccaa aaaataaaaa aatttgctgg agttatttaa
     5161 taaatatctt agcaaaataa taatattaca acttaatata aatatttatg aaaaagcaaa
     5221 taattggtat agtattaata gttgcaatgg taagttttag tggctgtaaa aaagctattg
     5281 aaaaaattaa agaacaggaa attgtaaatg ccattactaa tggtaaatgg gtagtttcta
     5341 aatttgtaga aggcactact accaccacta ctgactttgc aggatgggaa acaatttatt
     5401 atgaaaatgg cacatttaca agttctaaaa ctggtgaaac aaatcaaaca ggtacttggg
     5461 caggaaatgc ttctaattgg acatttacaa ttacttttaa tgcaacacca ccaacaccac
     5521 taaataaatt aggaggtgtt tggacagtaa cgagagcagt ttctacagat aaaggcagtt
     5581 atgcaaaaac cgaagctggt gtaaattatg aactagaaat gacaaaaatg ccttaaaggc
     5641 tatattaagt tttaaaaaat agatgataaa gttttgtagt attataccaa ataaataact
     5701 taaaacaatt taaaccatat atccatcttt catgtgcaat actctattgc ttaaagatgc
     5761 taattcttcg ttatgggtaa caattaaaaa ggtttgatga agatttgtgc atagttgtgt
     5821 aaataaatga tgcaactctt ttgcattttt actatctaag tttcctgttg gctcatctgc
     5881 aaaaactatt gatggtttat taattaatgc tcttgctact gctacacgtt gctgttctcc
     5941 tccactaagt tgattgggtt tatggtttaa cctattgctt aaacctaaaa tatctaataa
     6001 ttgtgcagcc tcttttttaa cctcggttgt ttttcttttt gcaatccatg ccggaatata
     6061 aacattttca atagcagaaa actcgggtaa taaatgatga aactgaaaaa taaaaccaat
     6121 atgcatattt ctaaatgcag ctaatttttt accacttaat aaatcaaccc taatatcatt
     6181 caaccatatt tcgccactat ctgctttatc taatgttcct aaaatatgca gcaaagtgct
     6241 tttaccagca ccactactac caactatact aacaatttgg ttgctttcta ttgcaatatc
     6301 aatacctttt aaaatgggta attcgttata ttttttggtt atattttttg cagataataa
     6361 aaaatttgac ataaattatt tcaataatgg tatagaaatg tataacaact ttgtttaaaa
     6421 ataatgaata ttaaaaacta aaaagcaatc taaataaaga ttgctttttt caaaaaaata
     6481 ttttaaaat
//
LOCUS       DAOQHP010000083         6486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6422_length_6486_cov_3.407557, whole genome shotgun sequence.
ACCESSION   DAOQHP010000083 DAOQHP010000000
VERSION     DAOQHP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6422_length_6486_cov_3.407557"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLY81_03945"
     CDS             <1..798
                     /locus_tag="PLY81_03945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF29409.1"
                     /translation="KIIYTNNVKEANKIKAKLIVGADGDHSVVLRSLGERSVNREHYA
                     GGLRQYWKGIEGISSEDNFLELYLPKSLPLAYLWIFPLPNGEANVGCGLLSSLIQKNN
                     VNLKNLLQDIITKDPVLAYRFRNAVPLEKPMGWGLPMATLKRKASGNGWLLTGDAASL
                     INPLTGEGIGPGMMSGYIAAHFIQRAVKQNNFSEKLFKNYDREINRRLEKSITNFNLI
                     KKVSPLIYNFFINVVAPHPLCRLYFQRNVGKWIKTAYDNKPIKIKFD"
     gene            complement(809..1942)
                     /gene="tgt"
                     /locus_tag="PLY81_03950"
                     /pseudo
     CDS             complement(809..1942)
                     /gene="tgt"
                     /locus_tag="PLY81_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
     gene            2052..2870
                     /locus_tag="PLY81_03955"
     CDS             2052..2870
                     /locus_tag="PLY81_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF29410.1"
                     /translation="MNYKLISLSVVIATLLIACQTNSITGRSQLSLVSETEVQTMAKT
                     QYRQFLSQSKVVSTNTSKDAEMVNRIGKRISTAITKYYTEQGLSNELIGYEWEYHLVD
                     SKEVNAWCMPGGKIVVYTGLLPVTQNEAALAVVMGHEVAHALARHGNERMSQGLLQQL
                     GGVALSTALSNKPQQTQNLFMSAYGISSQVGFMLPHSRKQELEADKFGLRYAALAGYN
                     IREAIPLWERMEKMSAGQKTPEFLSTHPQEERRIKDLQKIMDETIRDYYRPISK"
     gene            3011..3661
                     /locus_tag="PLY81_03960"
     CDS             3011..3661
                     /locus_tag="PLY81_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF29411.1"
                     /translation="MSNLSLFEKLTLQDEKNLLIQGVPSGIEKQFSKLSYAKNVTPLL
                     KIKKVDFALVFAVNKIQLNNVVREVLCVLRDNSKFWIACPKPTSKIVSDLNRDCSWDV
                     LKNNGYERVETVDIDHMWSAVQFKRNLELPIVQPEPKIESAIAFEKSLIHYPIELERI
                     FAKHKNVKDFFVSLPTTNQKEYLSWIQAAKRADTRQRRLDVVLEKLMAGKKKPSEK"
     gene            3733..4959
                     /locus_tag="PLY81_03965"
     CDS             3733..4959
                     /locus_tag="PLY81_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF29412.1"
                     /translation="MNTKTLIVIGGGAAGFFCAINAARYNKNLKIIIVEKTNKLLSKV
                     KVSGGGRCNVTHDCYEINELIKHYPRGNNFLKKAFHWFNTTHTLEWFKAEGIELKKEE
                     DGRMFPKTNSSQTIIDCLLHLANYYNIEILLSSDVIKINKLNDLFTLQLANNKVLNCN
                     FLCIATGGYSKALQFKWLEDIGHTIEKPVPSLFTFNIPNNTITTLMGISVEYATIKII
                     GTKFQYSGPLLITHWGISGPAVLKLSAFAAQELNLKNYQFKIQINWLKDWTEQSLRED
                     WNSFREQYSSNKIYNKNPFQLPNRLWQFILQQSSIKEDAQWANLLSKQQNCLIKNLTA
                     MDLEVNGKTTFKEEFVTCGGIKLNEIDVNTMQSKIIPHLFFAGEVLNVDGITGGFNFQ
                     NAWTTGFIAAKGIAES"
     gene            complement(5002..5661)
                     /locus_tag="PLY81_03970"
     CDS             complement(5002..5661)
                     /locus_tag="PLY81_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNF29413.1"
                     /translation="MLPYQEIIIRILLASFLGALIGLERERKHWAAGLRTHMMVCVGS
                     CLIMMVSAFGFKDILGTPNVTLDPSRIAAQVVSGIGFIGAGTILLLKQGTIRGLTTAS
                     GLWTVAAIGLATGGGMYFAAVFVTIIAIIILWALKPLELNYFKKKNKTLRIITSLNEE
                     NKQLLNKLISTSIITNLQFEKIEDEYIYILEFENDHSEEIEKIINQIKNFPFIKQVSW
                     S"
     gene            complement(5748..6308)
                     /locus_tag="PLY81_03975"
     CDS             complement(5748..6308)
                     /locus_tag="PLY81_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNF29414.1"
                     /translation="MKNNFTKLHRLLHWVTALAMMILFITGFLRMNWMSKTTIANAIN
                     YKTTDAPLTKEQIGGIIKTIREPMWQWHEIFAKVMIITLIVRIIYMVLKGIKFTNPFK
                     TNINFKEKIQGLTYVYFYVFVTISAFTGICLENGFFATYEDAIEDIHKWGLYWFPIFM
                     ILHIGGVYSAEHSNKKGITSKMISGE"
BASE COUNT         2364 a          944 c         1031 g         2147 t
ORIGIN      
        1 aaaattattt ataccaataa tgtaaaggag gcaaacaaaa ttaaagccaa attaattgtt
       61 ggtgccgatg gcgaccactc agtagtttta agaagtttgg gagaaagaag tgttaacaga
      121 gaacattatg ctggaggttt aagacagtat tggaaaggta tagaaggtat atcttcagaa
      181 gataattttt tagaattgta tttacctaaa agcttaccat tagcttattt atggattttt
      241 ccgttaccaa atggtgaagc caatgttggt tgtgggttgc tgagcagctt aatacaaaaa
      301 aataacgtaa accttaaaaa ccttttgcag gatattatta ctaaagaccc tgttttagct
      361 tatagattta gaaatgcagt accattagag aaaccaatgg gttggggctt acctatggca
      421 accttaaaaa gaaaagcaag tggtaatggt tggttactta caggcgatgc agcttcgtta
      481 attaatcctt taacaggaga aggaattggt ccaggaatga tgagtgggta tattgctgca
      541 cactttattc agcgtgctgt aaaacaaaat aatttttctg agaaattatt taaaaattat
      601 gatagagaaa taaatagaag gctagaaaaa agtataacaa acttcaatct aataaaaaaa
      661 gtatctcctt taatttataa tttttttata aatgtggtag ctccacatcc tttatgtcgt
      721 ttatattttc aaagaaatgt aggtaaatgg ataaaaactg cttatgataa taagcctata
      781 aaaataaaat tcgattgaag ataaaatttt ataaacgttg cttaattatt ggcaaaatat
      841 tattcttcca actataaaaa ttattactta atatttgatt tctggcttcg ttaactaacc
      901 ataagtaaaa agataggttt tgaagacttg caatttgtaa tcctaatatt tcatctgcaa
      961 caaataaatg ccttaaataa gctttagaat aaaaattact aatttctgaa ggcaagccag
     1021 tatcaattgg cgaaaaatct gttgcccatt tcttattttt tatattaata acaccttgtg
     1081 tagtaaataa cattccattt cttccatttc ttgttggcat cacacaatca aacatatcaa
     1141 cccctaaagc tatacattca ataatatccc atggggtacc aacacccatt aagtacctag
     1201 gtttttggta aggtaaattc tcgcagcaaa gagcagtaaa ttcatacatc atttcaatag
     1261 gttctccaac acttagtcca ccaatggcat tgcctgttgt gtgcaaacta cttacaaaat
     1321 cgcaagaagc ctttcttaaa tctttataag tgctaccttg tacaattgga aataaatttt
     1381 gagtttagcc atatttatca ggtgtttctg ataacctgtt tatgcaacgc tgcaaccatc
     1441 tatgagttaa ctccatactt ttttgtgcat aggtataatt actagggtaa ggtgggcatt
     1501 catcaaaagc cataattata tctgcaccaa tgcttctctc aatatccatt actttttctg
     1561 gtgtaaacaa atgcctacta ccatcaatat ggctttgaaa taaaacccca tcttctgtaa
     1621 tttttctgtt agcagccaac gaaaaaacct gatatccacc actatcagtt aatataggtt
     1681 tatgccaacc attaaattta tgtaagcctc cggctgcctc taaaatatct acacctgggc
     1741 gtaagtataa atgataagta ttgcctaaaa ttatttgagc ctgaacatca atacaaagtt
     1801 gttgttgcgt aactgctttt acgctaccta ctgtaccaac tggcataaaa attggagtaa
     1861 gtatttcgcc atggtctgta ataatttttc cggctcttgc taaattttca gtatgctgta
     1921 gttgaaatga aagttgaggc ataaaccaac aaaaatataa ttttaaattg tataacaatt
     1981 tatattaaaa ttattttcag aaatggggca tattttttta ttgattattt ttgcaaacta
     2041 ttttataaat tatgaattat aagttaataa gtttaagtgt tgtaatagct acgctattaa
     2101 ttgcttgtca aacaaactca ataacaggta ggtcgcaatt aagtttagta tccgaaacag
     2161 aagtgcaaac aatggcaaaa acacaatacc gccagttttt aagccaaagt aaagttgtta
     2221 gtacaaatac tagtaaagat gctgaaatgg taaatagaat tggtaaaaga atttctactg
     2281 caattacaaa atattataca gagcaaggat taagcaatga acttataggc tacgaatggg
     2341 agtatcattt ggtagatagt aaagaggtaa atgcttggtg catgccaggt ggtaaaattg
     2401 ttgtttatac tggtttgttg ccagtaacac aaaatgaagc agcattggct gttgtaatgg
     2461 ggcacgaagt tgctcatgca ttggcaaggc atggtaatga aagaatgagc caaggattat
     2521 tacaacaatt aggaggtgtt gcattaagta ctgctttatc taataaacca caacaaactc
     2581 aaaatttatt tatgtctgct tatggtatca gttcacaagt tggctttatg ttgccacata
     2641 gccgtaaaca agaattagaa gcagataaat ttggattaag atatgcagca ttagctggtt
     2701 ataatattag agaagcaata ccactttggg aaaggatgga aaaaatgagt gcagggcaaa
     2761 aaacaccaga gtttttaagt actcacccac aagaggaaag aagaataaaa gaccttcaaa
     2821 aaataatgga tgaaactatt agagattatt atagacccat atccaaataa tttattactg
     2881 attttcaata ggttaaaaaa tactggttgt taaaattaca tttctatctt taatagacta
     2941 aaaaaaatca ataaattatt ataactttgc acttcttttt ttacatcacc tgaaaaacca
     3001 attttaggat atgtctaact taagtttatt tgaaaaatta acattacaag acgaaaaaaa
     3061 ccttttaatt caaggtgtgc cttctggaat tgaaaagcaa ttttctaaac tatcttatgc
     3121 caaaaatgta actcctcttt taaaaataaa gaaggtagat tttgcattag tttttgcagt
     3181 aaacaaaata caattaaata atgtagtaag agaagtattg tgtgtactac gtgataatag
     3241 taagttttgg attgcatgcc ctaagccaac aagtaaaata gtaagcgatt taaatagaga
     3301 ctgcagttgg gatgtattaa aaaacaatgg ttacgaacgt gtagaaactg tagatataga
     3361 tcatatgtgg agtgctgtac aatttaaaag aaatttagaa ttaccaattg tacaacctga
     3421 acctaaaatt gaaagtgcaa ttgcttttga aaaaagttta atacactacc caattgagct
     3481 tgaaagaatt tttgcaaaac ataaaaatgt aaaagatttt tttgtatcgc ttcctactac
     3541 caaccaaaaa gaatatttat cttggataca agctgctaaa agggcagata caagacaaag
     3601 aagattggat gtagttttag aaaaattaat ggcaggcaaa aaaaagcctt ctgagaaata
     3661 accccaaaac accttacaaa ttgtaaggtg tttttttttg ctattatacc ttgtagattt
     3721 gtataattag ttatgaatac aaaaacctta attgttattg gtggcggtgc tgctggtttt
     3781 ttttgtgcca ttaatgctgc tagatataat aaaaatctta aaataattat tgtagaaaag
     3841 acgaataaac tgctaagtaa agtaaaagta agtggtggtg gaagatgcaa tgtaacacat
     3901 gattgttatg aaataaatga attaataaaa cactatccaa gaggtaataa ttttttaaaa
     3961 aaagcatttc attggtttaa tactacacat actttagaat ggtttaaagc cgaaggaata
     4021 gagttgaaga aggaagaaga tggtagaatg tttcctaaaa ccaacagttc tcaaacaatt
     4081 atagattgcc ttttacattt agccaattac tataacattg aaattttgtt aagcagcgat
     4141 gttattaaga taaataaact taatgatctg tttaccctgc aattagcaaa taataaagta
     4201 ttaaactgta attttttatg tattgcaaca ggtggttata gcaaagcctt gcagtttaaa
     4261 tggcttgaag atataggcca tacaatagaa aaacctgttc catctttatt tacttttaat
     4321 attcctaata atacaataac aacattaatg ggcataagtg ttgagtatgc aaccataaaa
     4381 attattggca caaaatttca atatagtgga ccattgttaa taactcactg gggtataagt
     4441 ggcccagcag ttttaaaatt atctgctttt gctgctcagg agttaaacct taaaaattat
     4501 caatttaaaa ttcaaattaa ttggttgaaa gattggacag aacaatcctt aagagaagat
     4561 tggaatagct ttcgtgaaca atattcctca aataaaattt ataataaaaa tccttttcaa
     4621 ttacccaata gattatggca gtttatactt caacaaagta gtattaagga agatgcacaa
     4681 tgggccaatt tattaagtaa acaacaaaat tgtttaataa aaaaccttac tgccatggat
     4741 ttagaggtga atggtaaaac tacttttaaa gaagagtttg taacctgtgg tggtattaaa
     4801 ttaaacgaaa ttgatgtgaa tacaatgcaa agtaaaatta taccacattt gttttttgca
     4861 ggagaagtac taaatgtaga tggtattaca ggaggtttta attttcaaaa tgcatggaca
     4921 actggcttta ttgcagcaaa gggaattgca gaaagttaat ttatactatt tactggtttg
     4981 taatttttta aaattggata attaactcca tgaaacctgt tttatgaaag ggaaattttt
     5041 aatttggtta attatttttt caatctcttc tgagtggtca ttttcaaatt ctaaaatata
     5101 aatatattca tcctcgattt tttcaaattg taggttggtg attatagatg tacttataag
     5161 tttattaagt agctgcttat tttcttcgtt taagcttgta ataattctta gtgttttatt
     5221 ttttttctta aaataattta gttctaaagg ttttaaagcc cataaaataa taatagcaat
     5281 aattgtaaca aatactgctg caaaatacat tccaccgcct gttgctaggc caatagcagc
     5341 cactgtccat aaaccagagg ctgttgttag tccacgtatt gtaccttgct ttaataataa
     5401 aatagttcct gcaccaataa aaccaattcc actaacaact tgtgctgcaa ttctagaagg
     5461 gtcgagtgta acgtttggtg tgcctaaaat atccttaaat ccaaaagcag aaaccatcat
     5521 tattaaacaa gaacctacac ataccatcat gtgtgttctt aagcctgctg cccaatgttt
     5581 tctttctctt tctaaaccaa ttaaagcacc taaaaatgaa gcaagtaaaa tacgaataat
     5641 tatttcttga taaggtaaca taaagtttgg agttttgaat aaagtgaata ctccaaaata
     5701 ggatacaaaa attatgctaa taaatcaagt agtaaaagag ccatgcatta ttcaccactt
     5761 atcattttag aagttatacc ttttttatta gaatgttcgg ctgaatatac tcctccaata
     5821 tgtaatatca taaaaatagg aaaccagtac aagccccatt tgtgaatatc ttcaatggca
     5881 tcttcatacg ttgcaaaaaa accattttct aaacaaatac ctgtaaatgc agatatagtg
     5941 acaaacacat aaaagtaaac ataagttaag ccttgaattt tttctttaaa attaatattt
     6001 gttttaaatg gattagtaaa ttttatccct tttaaaacca tatatataat tctaacaatt
     6061 aaagttataa tcattacttt ggcaaatatt tcgtgccatt gccacatagg ttctctaata
     6121 gttttaatta ttccaccaat ttgttcttta gttaagggtg catcggttgt tttgtaatta
     6181 attgcatttg caatggtggt tttactcatc caattcattc ttaaaaaacc tgtaataaac
     6241 aaaatcatca tagccaatgc agtaacccaa tgtaacaaac gatgtaattt ggtgaaatta
     6301 tttttcatat tataaattta ttattcaaaa aagttaccaa agctttgata agattgttca
     6361 atttttgaaa caatttcttt tgcttttgtt gcatctgtag ttgcctccaa atcttctaca
     6421 agttttaaat gaggctctaa ccatttatgt aattcatcgt ggcttttgcc tgtcatggtg
     6481 cagctt
//
LOCUS       DAOQHP010000084         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6445_length_6473_cov_4.628856, whole genome shotgun sequence.
ACCESSION   DAOQHP010000084 DAOQHP010000000
VERSION     DAOQHP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_6473_cov_4.628856"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLY81_03980"
     CDS             <1..503
                     /locus_tag="PLY81_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29415.1"
                     /translation="ANSFANRAFSVDSSHADACYVIALVNSNYILIEKENKALGAYIN
                     TTKQFAEKAIQLNNKHAKAHFILGNWYLEMVNLSGLKKTAVKLFYGTLPKYSLEDAIK
                     NLELCRLYDQYYMINYLQLAKAYIQDNNPTKAIEVLQKLKKLPLRTADDAAIKEEGVS
                     LLSSLQ"
     gene            513..773
                     /locus_tag="PLY81_03985"
     CDS             513..773
                     /locus_tag="PLY81_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNF29416.1"
                     /translation="MDIKQLFELAVTNSKTLTEKPNNEILLQLYSLYKQATEGDNKED
                     APSNPFDFVAKFKHEAWTKLKGLSKETAMQQYIDLVNNLKNG"
     gene            907..3558
                     /locus_tag="PLY81_03990"
     CDS             907..3558
                     /locus_tag="PLY81_03990"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29417.1"
                     /translation="MLKKSLLIILAVFQLTSLFAQALYPVSIQEKAANSTLIIEGKVV
                     ERTSFWNTAHTMIFTSNKIKIYKVFKGEVAADFVEVLTQGGSVGNDAIHASDLLDLVW
                     GQEGIFFCHPNVINLKSPNTNNSLLEVYSSSQGFLNYNILEDKATDPFNVYNGISTNL
                     YPLIMQLTGQSFVIKDNTFNVNQQKQNLANKTSAPSISSFTPETVNAGAMLDPTNNLL
                     TINGTGFGTFTGNAAVIFNDANTIAGTDYLVAASNPQLSNLVVSWTDTEIKIKVPARA
                     GSGAIKVRDNLGNVAYSFTNLTAHYGVINTTFNSGGTYYVKEFNLMDVNSAGGYTVYY
                     STSTSGGGVNLDISPTKQTFQRALTTWREISGFNVIEGGTTTTQVITGSTSECVIMYD
                     NTNTGVSPLSAGTLAVCYSYSGICNVTNEARKPKFDVIIRNNGVSSGTTNFNEGPCPS
                     IAVNNSYIDLESVLLHELGHALNLAHVNLPSEGAGAGTTNPGNIMHYSVHGSNRRITP
                     DKSARDGATYSIQQQNNVYGNCTSQTEMVPLTIITEPKDECPLTFPSTPTDGGTIVNF
                     DMVHATSNRFVDPYFTQIKCDGSGSNVTSTAFYPILTDNSGNLNISITNYIATPTSLT
                     LCSSVIPGQLGTGFRFALYEVSTCPSAGAYPTPISCTTFTKNMVFNPITGLSANTNYL
                     IFISAIENTKANFNMVISGSALPIKLSSFNGKVQRDYNNLDWTADQIINVNKIEIERS
                     STGSDFTTIGTIDGAAVYNKVGNFKDYKPTINSYYRLKIINNDGSVEYSKTINLKRTE
                     KVLFSISPNPATTYADVQISSEVKGKYSLVLYNLNGQRVYSKAINLNQGLNNHKINTV
                     TFAKGVYRLILMNENNNNVYSNSLIVQ"
     gene            complement(3708..4802)
                     /locus_tag="PLY81_03995"
     CDS             complement(3708..4802)
                     /locus_tag="PLY81_03995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNF29418.1"
                     /translation="MIIISCNNNNKSKVNNHLFPFRGLSKIEKLDFSSKALNIYNSIL
                     GNEFSGGILIAKNGEIVFEKYNGFINFKEKEHIKSSTPIHIASISKPITALAILQLVE
                     KKLISLDDTIQKFFPSFPYQNITVLHLLNHRSGLPNYLYFMDTAWRASTKFVTNNDVL
                     QFMIKNQPAAYSNPNTVFHYCNTNYVLLAIIVESVTHQFFPNYIKENIFKPIGMQNSF
                     VFNINDTSNYVPSYQFNNIPYTLESFDCVYGDKNVYSTVRDLWLLDKALYNNSIISKN
                     TFNIATTAYSNETKGNKNYGLGWRLFLKNNDTVIYHGGWWHGNNSLFTRLIKDTATVI
                     TLGNRYNKRIYNSKQMATIFSNKEIGEVEE"
     gene            4928..6169
                     /gene="serA"
                     /locus_tag="PLY81_04000"
     CDS             4928..6169
                     /gene="serA"
                     /locus_tag="PLY81_04000"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNF29419.1"
                     /translation="MNKKLQNTSYPKEKINILFLENISEKAVQILKQHGYVNVKKNSG
                     ALSEEELIKAIKDVHVLGIRSKTFITEKVLQAANKLQAIGCFCIGVNQVNLQAATNKG
                     VVVFNAPYSNTRSVAELVIGSAIVLIRKIIDKNNAAHKGIWLKDSKGSYELRGKTLGI
                     IGYGNIGSQVSVLAESLGMKVMFYDVETKLPLGNATVCKNLKELLSVSDIISLHVPET
                     PQTKNLINKNNLKYFKNGCILINYARGEVVDLDALATALKEMQLSGAAVDVFPVEPEK
                     NGDTFKSPIQGLNNVLLTPHIGGSTEEAQQNIGEDISYKLIHFLESGITNGSHTVPAI
                     ILPPVDSAHRILHIHKNVPGVLSAINTSLSKHNINIVGQHLKTNDDIGYVVLDVDKKL
                     SKHAVELLKEIKETIKVRMLY"
     gene            complement(6166..>6473)
                     /locus_tag="PLY81_04005"
     CDS             complement(6166..>6473)
                     /locus_tag="PLY81_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNF29420.1"
                     /translation="CIGGDYKLPNSTYDNFYWSTDVGKKWASPNTAPPYGYRSCLRII
                     DKETMIACGLNGVDISKDGGDDWKNISKEGFNVCMVSAKNKMVFLAGDRGKVGIYYY"
BASE COUNT         2401 a          972 c          950 g         2150 t
ORIGIN      
        1 ctgctaattc ctttgcaaac agagcatttt cggtagattc atcacatgca gatgcctgct
       61 atgttattgc cctagtaaat agtaactata ttttaataga aaaagaaaat aaagcattag
      121 gagcctacat taatactaca aaacaatttg cagaaaaagc aattcaactg aacaacaagc
      181 atgcaaaggc acattttatt ttaggtaatt ggtatttaga aatggttaac ttatctggcc
      241 ttaaaaaaac tgctgttaaa ttgttttatg gtacattgcc aaagtatagt ttagaagatg
      301 ccattaagaa tttagaacta tgccgtttgt acgatcaata ttatatgata aattatttac
      361 aacttgctaa agcatatata caagataaca accctaccaa agcaatagaa gttttacaaa
      421 agttaaaaaa acttccatta agaacagccg acgatgctgc aattaaagaa gaaggcgtca
      481 gtttattatc atcattacaa taataaaaca atatggatat aaaacaatta tttgaattag
      541 ctgttactaa cagtaaaact ttgacagaaa aacccaacaa cgaaatttta ttacaacttt
      601 atagcctata taaacaggca acagaaggag ataataagga agatgcacca tctaacccat
      661 tcgattttgt tgctaaattt aaacacgaag cttggacaaa attaaaggga ctaagtaaag
      721 aaactgctat gcaacaatat atagatttgg taaacaattt aaaaaatggg taaatacaat
      781 actttataat gcagcataaa aagctattca agtatcaaaa tactagcttt attactagtt
      841 agtaactaat tacaatatgg atactaaatt ttacaaatta ttttatcttt gctacaaatt
      901 acatttatgt taaaaaaatc tttactcatt attcttgcag tttttcaact aacaagttta
      961 tttgctcagg cactttatcc tgtatcaatt caagaaaaag ctgccaattc taccttaatt
     1021 attgaaggta aagtagtaga aagaacttct ttttggaata cggcacatac catgatattt
     1081 acttccaata aaataaaaat ttacaaagtt tttaaaggag aagtagctgc cgattttgta
     1141 gaagtattaa ctcagggtgg ttcagtaggc aacgatgcca ttcatgcaag tgatttatta
     1201 gacttagttt gggggcaaga gggtatattt ttctgccatc ccaatgttat taatttaaaa
     1261 tctcctaata caaataatag tttattagaa gtttattcaa gctcacaagg gtttttaaac
     1321 tataatattt tagaagacaa agcaaccgac ccatttaatg tatataatgg aatatctacc
     1381 aacttatatc cattaattat gcaactaaca ggtcaatctt ttgttattaa agacaataca
     1441 tttaatgtaa atcagcaaaa acaaaattta gccaataaaa catctgcacc atctataagt
     1501 tcatttacac cagaaactgt aaatgcaggt gcaatgttag atcctacaaa caacttatta
     1561 accattaacg gtactggctt tggaactttt actggaaatg ctgctgttat ctttaacgat
     1621 gcaaacacaa ttgccggaac tgattattta gttgctgctt ctaacccaca actttccaac
     1681 ttagtagttt cctggaccga tactgaaatt aaaattaaag tacctgcaag agctggtagt
     1741 ggtgcaataa aagttagaga taatcttggt aatgttgcat actcctttac caatttaact
     1801 gcacattatg gtgtaattaa taccactttt aactcaggtg gtacatatta tgtaaaagag
     1861 tttaatttaa tggatgttaa tagtgccggt ggttatacag tttattatag tacaagtact
     1921 tctggtggag gtgtaaattt agatattagc ccaaccaaac aaacttttca acgtgcatta
     1981 acaacttgga gagaaatttc tggctttaat gttatagaag gtggtactac aacaactcaa
     2041 gtaattacag gttctacatc agaatgtgtt attatgtacg ataataccaa cactggcgtt
     2101 tcacctttat ctgcaggtac acttgctgta tgctattctt actctggcat ttgtaatgtt
     2161 actaatgaag ctagaaaacc aaaatttgat gttattatta gaaataatgg cgtatcttct
     2221 ggaacaacta attttaatga aggaccttgc ccttcaatag cagtaaataa ttcttatata
     2281 gatctagaat cagttttact acatgaatta ggccatgctt taaaccttgc tcacgttaac
     2341 cttccttcag aaggtgctgg tgcaggtaca actaatcctg gtaatattat gcactattca
     2401 gttcatggat ccaatagaag aataacacca gataaatcag ctagagatgg tgctacatac
     2461 tccatacaac aacaaaataa tgtatatggt aattgtactt ctcaaacaga aatggtacct
     2521 ctaactataa ttactgaacc aaaagatgaa tgtcctttaa ccttcccttc tacaccaaca
     2581 gatggcggta caattgtaaa ttttgatatg gttcatgcaa ctagtaacag atttgttgac
     2641 ccatatttta cccaaattaa atgcgatggt tctggaagca atgttaccag tacagcattt
     2701 tatcctattc ttactgataa tagtggtaac ttaaatattt caattactaa ctatattgca
     2761 acaccaactt cattaactct atgctcttct gttatacctg ggcaacttgg tacagggttt
     2821 agatttgctt tatacgaagt aagtacttgt ccttcagcag gtgcatatcc aaccccaatt
     2881 tcttgtacaa cttttacaaa aaacatggtg ttcaatccta taacaggatt aagtgctaat
     2941 acaaattatt taatttttat aagtgctata gaaaatacta aggctaattt taatatggtt
     3001 attagtggtt cagcacttcc tataaaacta agctcattta atggtaaagt acaacgtgat
     3061 tataataatt tagattggac tgcagaccaa attattaatg taaataaaat agaaattgaa
     3121 agaagtagca caggatcaga ttttacaaca ataggcacaa ttgatggggc tgctgtttat
     3181 aataaagtag gaaactttaa agattataag ccaacaataa atagttacta tcgtttaaaa
     3241 ataattaata atgatggtag tgtagagtat tcaaaaacaa ttaatttaaa aagaaccgaa
     3301 aaagtattat tttctatttc accaaaccct gcaactactt atgcagatgt acaaatttct
     3361 agcgaagtaa aaggtaaata ctctttagtt ttatataact taaatggcca aagagtttat
     3421 agtaaagcaa ttaacttaaa ccaaggttta aataaccata aaattaatac agtaacattt
     3481 gctaaaggtg tttatcgttt aatattaatg aatgaaaata ataacaatgt ttattctaat
     3541 tcgttaattg ttcaataagt ttttttcaag tattttaaac aggttactaa ctatttagta
     3601 acctgttttt tttgatagta gataaaaata aaggctttta atttttaata aaaaatacta
     3661 actaaaaagt acattaagct tatcaataag tgcaatttta gttataacta ttcttcaact
     3721 tctcctattt ctttattaga aaatatagta gccatttgtt tgctattata aattcttttg
     3781 ttatacctat tacctaaagt aataacagta gcagtatctt taattaacct agtaaataaa
     3841 ctattattac catgccacca accaccatga taaataactg tatcgttatt ctttaaaaat
     3901 aaacgccagc ctaaaccata atttttattt cctttagttt cattactata ggcagtagta
     3961 gctatattaa aagtattttt ggagataata ctattattgt acaaagcctt atctaataac
     4021 cataaatctc taactgtaga atatacattc ttatctccat aaacacagtc aaagctctct
     4081 aatgtatagg gtatattatt gaattggtaa gaaggaacat aattggaagt atcatttata
     4141 ttaaacacaa agctattttg cataccaatt ggtttaaata tattttcttt aatataatta
     4201 ggaaaaaatt gatgtgtaac agattcaaca attatagcta ataaaacata attggtgtta
     4261 caataatgaa aaacagtgtt tgggtttgag taagcagcag gttggttttt aatcataaat
     4321 tgtaacacat cattattggt tacaaatttt gtagaagccc tccaagcagt atccataaaa
     4381 tataaataat ttggtaagcc acttctatga tttagtaaat gaagtactgt tatattttga
     4441 taaggaaagc tagggaaaaa tttttgaata gtatcgtcta agctgattag ctttttttca
     4501 acaagctgta atattgctaa tgcagtaata ggtttagaaa tagatgcaat atgaatagga
     4561 gttgagcttt taatatgttc cttttcttta aaatttataa agccattgta tttttcaaaa
     4621 actatttctc catttttggc aattaaaatt cccccactaa attcattccc taaaatgcta
     4681 ttataaatgt ttaatgcttt actcgaaaag tctaattttt cgattttact taagccccta
     4741 aatggaaata aatggttgtt aactttgctt ttattattat tgttacaaga tataattatc
     4801 atccccaata ttaatagcac tactctaatc ataattttaa aaaatatact ttgtttaaag
     4861 ccaacaaata tagtttagca ccaatgtatt gttccataaa ttgttcacaa atcgtttaat
     4921 ttgctgtatg aataaaaaat tacaaaatac tagttatcca aaagaaaaaa taaatatcct
     4981 ttttttagaa aatattagcg aaaaggctgt tcaaatttta aagcagcatg gatatgttaa
     5041 tgtgaaaaaa aattctggag cattaagtga agaagagtta attaaagcca ttaaagatgt
     5101 tcatgtttta ggcataaggt caaaaacatt tattaccgaa aaagtattac aagctgcaaa
     5161 taaattacaa gctattggtt gtttttgtat tggagtaaat caggtaaatt tacaagcagc
     5221 aaccaataaa ggggttgtag tatttaatgc accttacagt aataccagaa gtgtggcaga
     5281 attagtaatt ggctctgcaa ttgtattaat tagaaaaatt attgataaaa ataatgctgc
     5341 tcataaaggt atttggttaa aagattctaa aggaagttat gagttaagag gtaaaacatt
     5401 gggtattatt ggatatggta atattggtag tcaggtaagt gtactggcag aaagccttgg
     5461 tatgaaagta atgttttacg atgtagaaac taaacttcca ttaggtaatg caactgtgtg
     5521 taaaaattta aaagagttat tgtctgtttc cgatattatt tcattgcatg taccagaaac
     5581 tcctcaaacc aaaaatttaa taaataaaaa caaccttaaa tattttaaaa atggatgtat
     5641 attaataaat tatgccagag gcgaagtagt agatttagat gctttagcta cggcattaaa
     5701 agaaatgcaa ctaagtggtg cagctgtaga tgtatttcct gttgaacctg aaaaaaatgg
     5761 cgatacattt aaaagcccta ttcaaggctt aaacaatgta ctgctaacac cacatattgg
     5821 aggtagcacg gaagaagctc aacaaaatat tggagaagat atcagttata aactcattca
     5881 tttcttagaa agtggaatta ccaatggctc tcacactgta cctgcaatta ttttaccacc
     5941 tgtagattct gcacacagaa ttttacacat acacaaaaat gtaccaggtg tattaagtgc
     6001 cataaataca tctctatcta aacataatat aaatattgta ggacaacacc ttaaaaccaa
     6061 tgatgatatt ggttatgtgg tattagatgt agataaaaaa ttatctaaac atgcggttga
     6121 attattaaaa gaaataaaag aaactattaa agttagaatg ttgtattagt agtagtaaat
     6181 cccaactttt cctctatcgc cagctaagaa aaccatttta ttttttgcag ataccatgca
     6241 aacattaaaa ccttctttag agatgttttt ccaatcatct ccaccatctt tactaatatc
     6301 aacaccattt agcccacagg ctatcatggt ttctttatct attatacgta aacaacttct
     6361 gtatccataa ggaggtgctg tattgggaga agcccatttt ttcccaacat ctgttgacca
     6421 ataaaaatta tcgtaagtac tatttggtaa tttgtaatct ccaccaatac aat
//
LOCUS       DAOQHP010000085         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6605_length_6380_cov_3.901976, whole genome shotgun sequence.
ACCESSION   DAOQHP010000085 DAOQHP010000000
VERSION     DAOQHP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6605_length_6380_cov_3.901976"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLY81_04010"
     CDS             <1..709
                     /locus_tag="PLY81_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF29421.1"
                     /translation="MFQNSMMRLRYSSIPVVTAPHGLTLGGGCEMNLHADKICAAAET
                     YIGLVELGVGLIPGGGGTKEFVLRASDELHKDEPETNTLKDRFFSIATAKVATSAAEA
                     FTMGILRKGQDEVVMNMGRRIAEAKKSVIELFDQGYVMPQQRKDIKVLGRLGLGAMLA
                     GINGMQRANYATEHDAFVAKKLAYVMCGGDLSEPQFVTEQYLLDLEREAFLSLCGEKK
                     TLERIQGVLKNGKPVRN"
     gene            1019..1633
                     /locus_tag="PLY81_04015"
     CDS             1019..1633
                     /locus_tag="PLY81_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29422.1"
                     /translation="MHFVPNNLYHIYNQGNNKQVIFHDEDDYYTFLRLTRNLFHSVCD
                     IVAYCCMPNHFHFIISTNGYSCEIKQQGNISIDAITNSIRKLLSSFARLYNKKYLQSG
                     SVFRQKTKSKCLTNCDIEVLTSMNGREYLFTCFHYVHQNPLRAGLVNKLEDWDFSSFK
                     DYAGLSSGTLINKEVAKFYDIYNPSNFIKESYMVISDEYIKFIV"
     gene            complement(1802..1963)
                     /locus_tag="PLY81_04020"
     CDS             complement(1802..1963)
                     /locus_tag="PLY81_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29423.1"
                     /translation="MKDSVTVKPFSSKEIIISLTPTSNIIYVEEEKPKSISIFSMDGK
                     EQTEKFVMN"
     gene            2171..2887
                     /locus_tag="PLY81_04025"
     CDS             2171..2887
                     /locus_tag="PLY81_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF29424.1"
                     /translation="MICKKEWQQFFSNLTGYIIIAVFLIVNGLVLFVFDDNIFSFGYA
                     TLEKFFMLAPWVLLLLIPAVTMRSLPDEYKLGTIEVLKTLPLTPWQIITGKYFGNLLV
                     IFLSLLPTFIYIFSIQNLADGNGLDLAATIGSYIGLFFLAAVFTAIGICAGSFTNNAV
                     VAFIISLFACALLYYGFNAISKLSQLSGGIDYYLEMAGIDFHYKNISKGVIDTRDVVY
                     FLSIIFLLLLITVRNLKHKI"
     gene            2920..4623
                     /gene="gldG"
                     /locus_tag="PLY81_04030"
     CDS             2920..4623
                     /gene="gldG"
                     /locus_tag="PLY81_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNF29425.1"
                     /translation="MQKLLQHKFSWLLLIAAFAIVNFMASIVHTKIDLTKEKRYTISK
                     ATKDILQHIDDKLEITVFLEGNLPAGFQKLANTTNDFLQLLKEKNNAKITFQFISPDT
                     EVDGTNIKFEDTLVAMGATPINLTIQVKQGQESKVVFPYALIKYKTHTQLISLYSGNS
                     RMISQDEINNAEAMMEYKFLKAFDAFVFPEKPLVAYSFANGEPTDIRTYDLLQTLQKD
                     YSLYTFDIAQRPAIPDTFKVLMIVKPSLQFSEDEKLKIDQYLMRGGKILCFIDNLNAE
                     QDSLNFKPELVAYDRNLNLTDLLFKYGVRINTDLVMDLQCNFMPFAVGGAKENPQYEF
                     LHWNYYPLFESKNNHTINKNLGLVSGRFVNSIDTVKAEGITKTILLSSSDNSRKLGTP
                     ALISLNENRNTPQDILFQQKNIPVAVLLEGSFTSLYKNRLAQHIKDSVEKIGYPFLEK
                     SFSESKIIVVADGDIVLNDASQKLGPLPMGLNMYTVGTQYEYQFANREFLLNSLEYLT
                     NKQSIIQSRNKEQILRLLNTSKVEEEKVKWQMINIGLPLLFVIIYGWVYNLIRKKKFT
                     T"
     gene            4639..5598
                     /locus_tag="PLY81_04035"
     CDS             4639..5598
                     /locus_tag="PLY81_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNF29426.1"
                     /translation="MTTHQIVYLVFAVVLVVALIFDLGLLNKKNKEVSIKAALQQTML
                     WVSLALGFFVFMWVEEGQTLALQYLSAYLMEWSLSIDNIFVFILIFSAFSVKEKHYGR
                     VLLIGILMAIVFRIIFITVGVALVDRFEWILYIFGAFLLYTGIKMFTANHDEEFKPQE
                     SKIYRFMQRFLPLVPHDGGGKYIIREKGKPVYTTLFVVVIMLAAIDLVFALDSIPAVM
                     GISRDKLVIYTSNIFAVLGLRSLFFLLRGAVSKFDYLQQGIAIVLVFIGIKMLAEHYI
                     SMVVSKNIQVAISLGVILVCISGSIFYSIFMKKKGVPKDVEEE"
     gene            5611..6246
                     /locus_tag="PLY81_04040"
     CDS             5611..6246
                     /locus_tag="PLY81_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNF29427.1"
                     /translation="MSYTIHNIAIILKATATINNNTLIEQLLIDSRKITFPTASLFFA
                     LHSKVKNGHSFINEVYERGVRNFVIDEDIDSTPFTNANFLKVPNTLVALQALATHHRN
                     EFNQLQVIGITGSNGKTIVKEWLNQLLQTDFNIVRSPRSYNSQIGVPLSVWQINSTNT
                     LGIFEAGISTANEMDTLEKIIKPTIGILTNIGGAHNEGFEDDTHKFLEKIK"
BASE COUNT         2211 a          851 c         1083 g         2235 t
ORIGIN      
        1 aatgtttcaa aattccatga tgcgtttgcg ttatagcagt attcctgttg ttacagctcc
       61 acatggttta acattaggcg gaggctgcga aatgaattta catgctgata aaatttgtgc
      121 tgctgcagaa acttatattg gtttggttga gttaggtgta ggcttaattc ccggtggtgg
      181 tggcactaaa gaatttgttt taagagcaag tgatgaacta cataaagatg aaccagaaac
      241 caatacctta aaagatagat ttttttctat agcaactgca aaagtagcta caagtgctgc
      301 agaagctttt acaatgggta tattaagaaa aggacaagat gaagtagtaa tgaacatggg
      361 tagaagaatt gcagaagcaa aaaaatctgt aattgaattg tttgaccaag gttatgtaat
      421 gccacagcaa agaaaagata ttaaagtatt agggcgattg ggtttgggtg caatgctagc
      481 aggtattaat ggtatgcaac gtgctaatta tgctacagaa catgatgcgt ttgttgctaa
      541 aaaattagct tatgtaatgt gtggtggcga tttaagtgaa ccacaatttg taactgaaca
      601 atatttattg gatttagaaa gagaagcttt tttaagcctt tgcggcgaaa agaaaacttt
      661 agaaagaata cagggagttt taaaaaatgg caaacctgta agaaactaag atagtctgac
      721 cgcctcggtc tgcctatctt agtttctcat ctttatatta cagtcagacc gtctcggaca
      781 tactgtaaat ttcttcaggc cttcactgct tgcctatctt agtttttcac cttttattat
      841 attacagtct gaccgtctcg gtctgactgt aaatttttta tgaccttctc ggtcagacta
      901 tcttagtttc tcatctttat attacaatca gaccgtttcg gtcagactgt aaatctctta
      961 tcttgtatta tatatcagtc aaaatatttg gagcaaactg ttgtaaattt gttacactat
     1021 gcattttgtt cccaataacc tctaccacat atacaatcag ggaaataata aacaagtaat
     1081 atttcatgat gaggatgatt actatacttt tttacgatta acaagaaatt tatttcattc
     1141 tgtttgtgat attgttgcat attgctgtat gcctaatcat tttcatttta taattagtac
     1201 aaatggatat tcttgtgaaa taaaacaaca aggtaatatt agcattgatg ctattaccaa
     1261 ttcaatcaga aaacttttga gcagctttgc taggctatac aataaaaagt atctacaatc
     1321 tggctcggtg ttcagacaga aaacaaaatc taaatgctta acaaattgcg atatagaggt
     1381 tttaacatca atgaatggta gagaatattt gtttacttgt tttcattatg ttcatcaaaa
     1441 tccattaaga gcaggattag taaataagtt ggaggattgg gatttttcat cgtttaaaga
     1501 ttatgcaggt ttaagtagtg ggactttgat aaataaagaa gtagcaaaat tttatgatat
     1561 ttataatcct tccaatttta taaaagaatc atatatggta ataagcgatg aatatatcaa
     1621 attcatagtt tgaccaactc ggcctgacta tgttggtttt tcatcttaca ttatctatca
     1681 gtatgtcagt cagaccggga cggtcagact aatagcataa acttaaactt cttttcagtc
     1741 ttctcggtaa gaccgtctcg gtctgactat ggaaggattg agtaaacaat aatgtaatag
     1801 cttaattcat tacaaacttt tctgtttgtt ctttgccatc catagaaaat atgcttatgg
     1861 attttggttt ttcttcttct acataaataa tgttgctagt aggagtaaga cttattataa
     1921 tttcttttga agaaaaaggt tttacagtta cactatcttt catattttaa aaaattaaaa
     1981 tgatcaaaat attctctata acataattgt tttggtcttt aaataaataa gactattgtt
     2041 tttgttctgc atataattct gaagtaacaa atgaagtaat tgttaaattt gatttaattt
     2101 agttgtaaac ttttttcttt taaaataagc tactcaatct tttatcattt agttttacaa
     2161 acttatgttg atgatttgta aaaaagaatg gcagcagttt tttagtaacc taacaggcta
     2221 tataattatt gctgttttct taattgtaaa tggcttggtg ctatttgttt ttgatgataa
     2281 cattttttct tttggatatg ccaccttaga aaaatttttt atgcttgcac catgggtatt
     2341 gttattacta atacctgccg ttacaatgcg tagtttgccc gatgagtata aattaggtac
     2401 tatagaagtt ttaaaaaccc taccacttac accatggcaa ataataacag gtaaatattt
     2461 tggtaattta ttggtaattt ttctttcctt attacctacg tttatttaca ttttttcaat
     2521 acaaaacctt gctgacggaa atggtttaga tttagctgca actattggct cttatattgg
     2581 attgtttttt ttagctgcag tgtttacagc tattggtata tgtgcaggta gttttacaaa
     2641 taatgctgtg gtagctttta taattagcct ttttgcttgt gccttactgt attatggttt
     2701 taatgccatc agtaaattat ctcaattatc tggcggtata gattattatt tagaaatggc
     2761 aggaatagat tttcattaca agaacattag taagggtgtt atagatacta gagatgttgt
     2821 ttactttttg agtattattt ttttattact gttaattaca gtgagaaact taaaacataa
     2881 aatataatta gctaaactta gtattagatt attagaaaaa tgcaaaaact attacaacat
     2941 aaatttagtt ggcttttact aattgcagca tttgccattg ttaattttat ggcaagtatc
     3001 gttcatacta aaatagattt aactaaagaa aaaagatata ccattagtaa agccacaaaa
     3061 gacattctac aacatattga tgataaactt gaaataactg tttttttaga aggaaattta
     3121 cctgctggtt ttcaaaaatt ggcaaacact accaacgatt ttttacaatt attgaaagaa
     3181 aaaaataatg ctaagattac atttcaattt atatctccag ataccgaagt agatggcacc
     3241 aacataaaat ttgaagatac tttagtagca atgggtgcaa caccaattaa tcttacaata
     3301 caagtaaaac aagggcaaga gagtaaagta gtttttcctt atgcactaat aaaatataaa
     3361 acgcatactc aattaataag tttatatagt ggtaatagca gaatgatttc tcaagatgaa
     3421 attaacaatg ctgaagcaat gatggaatac aaatttttaa aagcatttga tgcatttgtt
     3481 tttcctgaaa aacctttagt agcttactct tttgcaaatg gagaaccaac agatattaga
     3541 acttacgatt tactacaaac attacaaaaa gattatagtt tatatacgtt tgatattgct
     3601 caaaggccag caataccaga tacttttaaa gtactaatga tcgtaaaacc atctttacag
     3661 tttagcgaag atgaaaaatt gaaaatagat caatacctca tgcgtggagg aaaaattttg
     3721 tgttttattg ataatctaaa tgcagagcaa gatagtttaa attttaagcc agaattagtg
     3781 gcttatgata gaaaccttaa ccttacagac ttattgttta aatatggggt aagaattaat
     3841 acggatttag taatggatct tcaatgcaat tttatgcctt ttgctgtggg tggtgcaaaa
     3901 gaaaaccctc aatacgaatt tttgcattgg aattattacc ctctatttga aagtaaaaat
     3961 aatcatacta tcaacaaaaa tttgggctta gtatctggtc gttttgtaaa ctcaatagat
     4021 actgtaaaag cagaaggtat aaccaaaacc attttacttt cttcttctga taattcaaga
     4081 aaattaggta cgccagcttt aattagttta aatgaaaaca gaaatacccc acaagatatt
     4141 ttatttcaac aaaaaaatat tccggtagca gttttattgg aaggtagttt tacctcttta
     4201 tacaaaaaca ggttggcaca acatattaaa gatagtgtag aaaaaattgg ttatcctttt
     4261 ttagaaaaaa gtttctctga aagtaaaata attgtggtgg cagatggaga tatagtttta
     4321 aatgatgcaa gccaaaaact tgggccacta ccaatgggct taaatatgta cactgtaggt
     4381 acacaatatg aatatcaatt tgctaacaga gagtttttgt taaatagttt agaatactta
     4441 accaataagc aaagtataat tcaatctcgt aacaaagaac aaattttaag attactgaat
     4501 acttctaaag tagaagaaga aaaagtaaaa tggcaaatga ttaatattgg gctgccttta
     4561 ctgtttgtaa ttatttatgg atgggtttat aacctcatca gaaaaaagaa attcaccact
     4621 taacttatat tttagttcat gacaacacat caaattgttt atttagtttt tgccgttgta
     4681 ctagtagttg cattaatttt tgacttaggc ctattgaata aaaaaaacaa agaagttagt
     4741 attaaagcag cattgcaaca aactatgtta tgggttagtt tagctctcgg cttttttgtt
     4801 tttatgtggg tagaagaagg tcaaacgctt gccttacagt atttaagtgc ttacttaatg
     4861 gagtggagtt taagtataga taacatcttc gtatttattc ttattttttc ggcttttagt
     4921 gtaaaagaaa aacattatgg cagggtttta ttaattggta ttttaatggc tatagttttt
     4981 agaataattt ttataactgt aggtgttgca ttggtagata gatttgaatg gatattatac
     5041 atttttggag cttttctttt atatacaggt attaaaatgt ttactgcaaa ccacgatgaa
     5101 gaatttaaac ctcaagaaag taaaatttat aggtttatgc aaaggttttt accattagta
     5161 ccacacgatg gtggtggtaa atatataatt agagaaaaag gtaaacctgt atatacaaca
     5221 ttatttgttg tggttataat gttagctgct attgatttag tttttgcttt agatagtatt
     5281 cctgcagtaa tgggtataag cagagataaa ttggtgattt atacttctaa tatttttgct
     5341 gttttagggt taagaagttt attcttttta ttgcgtggag cagtttctaa attcgattat
     5401 ttgcaacaag gtattgccat tgtattggtt tttattggta taaaaatgtt ggcagagcat
     5461 tatattagca tggtagtaag taaaaatatt caagtagcaa tatcattagg agtaatttta
     5521 gtatgtatca gcggctctat tttttattct atttttatga aaaagaaagg tgtaccaaaa
     5581 gatgttgaag aagagtagct taataataac atgagttata caatacataa tattgcaatt
     5641 attttaaagg ccactgcaac tattaataac aatactttaa ttgaacaatt attaattgat
     5701 agcagaaaaa taacctttcc tacagcatct ttattttttg ctttacacag caaagtaaaa
     5761 aatggacatt cctttataaa tgaagtatat gaacgaggtg ttagaaattt tgtaattgat
     5821 gaagatattg atagtacacc ttttaccaat gctaattttt taaaagtacc aaacacatta
     5881 gtagcgttac aggctctagc aacacatcac agaaatgaat ttaatcaatt acaagtaatt
     5941 ggaattacag gtagtaatgg aaaaactatt gtgaaagaat ggctaaacca attactacaa
     6001 acagatttta atatagtaag aagtccaaga agttataact cgcaaattgg tgtaccatta
     6061 agtgtatggc aaattaactc tactaacact ttaggcattt ttgaagctgg tatttcaaca
     6121 gctaacgaaa tggatacttt agaaaaaatt attaaaccaa caataggcat actaaccaat
     6181 attggtggag cacataatga agggtttgaa gatgatacac ataagtttct ggagaaaata
     6241 aaataaccat gccggccaga aaaaaagata gtttccaggt ttatgttaag cagatcgagg
     6301 caatttccaa ggttgccaac ctgatcactt ccgggatgta cttggaggaa ttattgcgtt
     6361 tggtggtgca agttactgcc
//
LOCUS       DAOQHP010000086         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6659_length_6341_cov_4.405982, whole genome shotgun sequence.
ACCESSION   DAOQHP010000086 DAOQHP010000000
VERSION     DAOQHP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_6341_cov_4.405982"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /gene="trxA"
                     /locus_tag="PLY81_04045"
     CDS             <1..267
                     /gene="trxA"
                     /locus_tag="PLY81_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNF29428.1"
                     /translation="PILVDFFATWCGPCKTMAPILEEVKKRVGEKATILKIDVDKNPQ
                     TAAAYQISGVPTLILFKNGKPQWRQSGVISAKDIETIINNFYNH"
     gene            278..658
                     /locus_tag="PLY81_04050"
     CDS             278..658
                     /locus_tag="PLY81_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF29429.1"
                     /translation="MKNFLLVFIISILLNACSAQDTAHYKVLSSSNYALALAQDKSAQ
                     LIDVRTPEEYEEGHLENAVNINIKDSNFIELVKKLNKEKPVYVYCRSGVRSRKAGAEL
                     EKLGFKQIFDLDGGILSWTGKTVK"
     gene            634..1629
                     /locus_tag="PLY81_04055"
     CDS             634..1629
                     /locus_tag="PLY81_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNF29430.1"
                     /translation="MDWKNSKVKILAAQKTYGIVLAVVIMLVAKFLAAYIPNLGVTIL
                     ALMLGIIVRQLIPNFSKFTPGVVWSEKYVLETAIVFIGFGFEINKFSKIGTSTILMIT
                     VSIIVVILISLLLGKIFGSNNNKLYWLLGAGSAICGSSAIGATAPLIEAKEEETGLSL
                     AVVNVLGLIGMILLPLIAAAFQFSSVDTGIFLGGILQSVGHVVSAGFAVSDEVGQFST
                     VVKMGRVAFLIPFLLIIFFLFRKRSNGTKIKLPLFILFFLIAVLISQLNIFNANAIKQ
                     ITKLGDGLLNIGMAAIGLKINIKSLWKISGKAFFAGGIIFIFQIILYFLFITVKL"
     gene            1752..2711
                     /gene="ric"
                     /locus_tag="PLY81_04060"
     CDS             1752..2711
                     /gene="ric"
                     /locus_tag="PLY81_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNF29431.1"
                     /translation="METTVENILNVTLLEPRLKHPTIFARFDELNEGESLTIHNDHDP
                     KPLYYQLLGQRGNIFTWDYLENGPEWWKIKIQKRIIGEGNETLGEIAANDIRKAQIFK
                     KYGIDFCCGGKKTVKEVCAEKGLDINVIEKELAEADKVVASRPLPYNDWSLDFLADYI
                     VNTHHSYVKQTLPDLKNYAAKVAQVHGSAHPELIPIYELVDAVYAEMSTHMVKEEQIL
                     FPYIKEIVGAKNNGTPIQFSSMGSVRGPINMMEMEHESTGKCLEEIRTLSNNFTLPSD
                     ACASYTLLFKLLEEFEEDLHIHIHLENNILFPKALEIEKKLNN"
     gene            2713..3036
                     /locus_tag="PLY81_04065"
     CDS             2713..3036
                     /locus_tag="PLY81_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF29432.1"
                     /translation="MQFKISQALPQCAEGKVKGFYGKDFINLQNGTVKLVRVDSFSKY
                     PLHLHPDKTEYAFVVKGNPFFVIDENKFTGEPGDFFIFPKNTKHAIENNTSNECVLLI
                     GAIIM"
     gene            3039..3848
                     /locus_tag="PLY81_04070"
     CDS             3039..3848
                     /locus_tag="PLY81_04070"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNF29433.1"
                     /translation="MIKINANTKISALLKAHPQALDAIISISSKFNKLKNPLLRKLMA
                     SRTSIAMASKIGGVQVADFFEKFKSLGFEIDETIAEKKTNNESNLPIFIQHISKENIK
                     ELDVRPVINSGNDPFNIILNSVKQLQKGDVLKLINSFEPAPLIPILNKQGFEAFVEHE
                     SSEVVNTYFYKINETEINSPDLTNNEEDWQSIIEKYSNNLVTIDVRNLEMPLPMLTIL
                     EEIEKIPNYKALYVYHKRIPVFLLPELKERNFDYRTKEITDGEVHLLIFKA"
     gene            3852..5141
                     /locus_tag="PLY81_04075"
     CDS             3852..5141
                     /locus_tag="PLY81_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HNF29434.1"
                     /translation="MFVGLGNSSIVKTTSYKVVLPFYLYAAISFIVASIFLLLSSNTF
                     LAHYFQPHILAITHIMALGWGTMIILGASHQLVPVLIEGELYSYKLAHLSFILSAIGI
                     PLLVYGFYVFDMGTPAKWGGRLVLLSILCYLINIGYSISKSKKENVHAVYVFTSVIWL
                     FITVSLGLALVYNFTYNFLPQNSLHYLPLHVHTGILGWFLLLVIGVASRLIPMFLISK
                     YTNPTILWIIFWLINTALISFIIIFFYLPLSYTLIPDYLLFVAIVLFIYYCYNAYKQR
                     IRKQVDEQVKLSLLSTVLMLLPILLLFIIIILFINSSAENYQPMVAYGFMIFFGWITA
                     IILGMTFKTLPFIVWNKIYHQRSNKGKTPNPKDLFSNTIFKIMSIAYILGIVIFLLGL
                     VFENAILLKFGAFFILVTSILYNWNVLKIVYHKPTTT"
     gene            5138..5461
                     /locus_tag="PLY81_04080"
     CDS             5138..5461
                     /locus_tag="PLY81_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNF29435.1"
                     /translation="MNLKTNNEEKSKLAIEGLYNVIDPEIGLNVVDLGLIYELHFDEE
                     ENRLTCTMTLTTEFCPMGESITDNARHALQQAYGDYGIDINLIFDPPWNMEMISDEGR
                     AFLGR"
     gene            5464..5895
                     /locus_tag="PLY81_04085"
     CDS             5464..5895
                     /locus_tag="PLY81_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF29436.1"
                     /translation="MLSLSCKAAIKAVIFLGSKFETEEKFSIKTIAGFINENEHTVGK
                     LLQKLVKENIINSTKGPNGGFFISAKQINQPVIKIVEAIDGKDIFKQCGLGLEKCSES
                     HPCPFHNNFKPIRDMFKKLCEEKKVIDLYQNVNEGLAYLTN"
     gene            5994..>6341
                     /locus_tag="PLY81_04090"
     CDS             5994..>6341
                     /locus_tag="PLY81_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29437.1"
                     /translation="MKTELLEFENKHKLLEPTSTERVATLEAISEYTENFLGNIDTLP
                     AFVNNVSEKAPFIQSINFEEQNTLNEVLKNIRLYIDTPGINPASGGHLGYIPGGGLFY
                     GAVGDYLACITNRY"
BASE COUNT         2268 a          860 c         1027 g         2186 t
ORIGIN      
        1 cctatattgg tagatttttt tgcaacttgg tgtggcccat gtaaaacaat ggctcctatt
       61 ttagaagagg taaaaaaacg tgttggcgaa aaagctacta ttttaaaaat tgatgtagat
      121 aaaaatccac aaactgctgc tgcttatcaa attagtggtg taccaacatt aattttgttt
      181 aaaaatggta agccacaatg gcggcaatcg ggtgttattt ctgcaaaaga tatagaaaca
      241 ataataaata atttctacaa ccattaaacc tttttacatg aaaaactttt tgttggtatt
      301 tataattagt atcttattaa atgcttgtag tgcacaagat acagcacatt ataaagtgtt
      361 atctagtagt aattatgcac tagccttagc acaagataaa tcggcacaat taatagatgt
      421 tagaacacca gaagaatatg aagaagggca tttagaaaat gctgtaaaca tcaatataaa
      481 agatagtaac tttatagaat tagttaaaaa actaaacaaa gaaaagcctg tttatgtgta
      541 ttgcagaagt ggtgtaagaa gtagaaaagc tggagctgaa ttagaaaaat tgggctttaa
      601 gcaaattttt gatttagacg gtggtatttt atcgtggact ggaaaaacag taaagtaaaa
      661 atattggcag cacaaaaaac ttatggaatt gttttagcag ttgtaataat gcttgttgct
      721 aaatttttag cagcttatat tcctaatttg ggcgttacta tacttgcact aatgttaggt
      781 attattgtaa ggcaattaat tcccaacttt tctaaattta caccaggtgt tgtatggtca
      841 gaaaaatatg tattagaaac tgcaattgtt tttatagggt ttggatttga aataaataag
      901 ttttcaaaaa ttggtacctc taccatatta atgattacag taagcattat tgttgttata
      961 ttaatttctc ttttattagg caaaattttt ggaagcaata acaataaact ttattggctt
     1021 ttaggtgcag gaagtgcaat ttgtggctcc tctgcaattg gagcaactgc cccattaata
     1081 gaggcaaaag aagaagaaac cggattatct ttagctgttg taaatgtact tggcttaatt
     1141 ggtatgattt tgcttccatt aattgccgct gcttttcagt tttcttctgt tgatactggt
     1201 attttcttag gaggtattct acaatctgtt gggcatgtag taagtgcagg gtttgcagtt
     1261 agcgatgaag tggggcaatt ttctactgtt gtaaaaatgg gtagagtagc ttttcttatt
     1321 ccttttcttc ttataatttt tttcctattc agaaaaagaa gtaatggtac aaaaattaaa
     1381 cttccattat ttatattgtt cttcttaata gctgttttaa tttctcaact taatattttt
     1441 aatgctaacg ctataaaaca aattacaaaa ctgggcgatg gtttattaaa tattggtatg
     1501 gctgcaattg gacttaaaat aaacatcaaa tctttatgga aaatttcagg caaagcattt
     1561 tttgcaggcg gtattatttt tatttttcaa attatcctgt actttttatt tataacagtt
     1621 aagttataat aaggcgagca attaactaca tcttctaaat gtcattcatt tgtcaataat
     1681 aaaagtattt aattactttt attgtaattc tcttaccttt gctgtattat taacaaaata
     1741 aaaaaaatgt gatggaaaca actgtagaaa acattctaaa tgtaaccctg ttagagccaa
     1801 ggttaaagca cccaactatt tttgcaagat ttgatgaact taacgaaggg gaaagtttaa
     1861 ctattcataa cgatcatgac ccaaagcctt tatattatca attattgggg cagagaggta
     1921 atatttttac ttgggattat ttagaaaatg gtccagaatg gtggaaaata aaaattcaaa
     1981 aacgcattat aggcgagggt aatgaaaccc ttggagagat tgctgctaat gatataagaa
     2041 aagctcaaat atttaaaaaa tatggaatag acttttgctg tggtggtaaa aaaacagtaa
     2101 aagaagtttg tgcagaaaaa gggcttgata taaatgttat tgaaaaagaa ttagcagaag
     2161 cagataaggt tgtagcctct cgtccattac cttataatga ttggagctta gactttttag
     2221 cagattatat tgtaaacacc caccatagtt atgtaaaaca aacattaccc gatttaaaaa
     2281 attatgctgc aaaagttgca caagttcatg gaagtgcaca tcctgagcta attcctattt
     2341 acgaattagt agatgctgtt tatgcagaaa tgagtaccca catggtgaaa gaagaacaaa
     2401 tcttatttcc atatataaaa gaaatagttg gtgcaaaaaa taatggaacc cctattcagt
     2461 ttagctctat gggttctgta agaggtccaa ttaatatgat ggaaatggag catgaatcta
     2521 ccggtaaatg tttagaagaa ataagaacac ttagcaataa ttttacacta ccaagcgatg
     2581 cttgtgctag ttatacttta ttgtttaaat tgttagaaga gtttgaagaa gatttacaca
     2641 ttcatataca tttagaaaac aatatacttt ttcctaaagc attagaaata gaaaaaaaat
     2701 taaacaacta aaatgcaatt taaaattagc caggccttgc cacaatgtgc agagggtaaa
     2761 gtaaaagggt tttatggtaa agactttatc aatttacaaa atggtacagt aaaactagtt
     2821 agggttgact cattttcaaa atatccatta cacttgcatc cagataaaac tgaatatgca
     2881 tttgttgtaa aaggaaaccc tttttttgtg attgatgaaa ataagtttac tggcgagcct
     2941 ggcgattttt ttattttccc caaaaacaca aaacatgcaa tagaaaataa tactagcaat
     3001 gaatgtgttt tattaattgg tgcaataatt atgtagaaat gataaaaatt aacgccaata
     3061 caaaaatttc tgctttatta aaagcacatc cacaagcatt agatgctatt attagtattt
     3121 catctaaatt caataaacta aaaaaccctc ttttaagaaa actaatggct tctcgtacat
     3181 ctattgcaat ggcaagtaaa attggtggtg tacaagtagc agattttttc gaaaaattta
     3241 aatccttagg gtttgaaata gatgaaacaa ttgcagaaaa aaaaaccaac aacgaaagca
     3301 atctcccaat attcattcaa catatttcta aagaaaatat taaagagtta gatgttcgtc
     3361 cggttattaa tagtggtaac gatcctttta atattattct taactcagta aagcaattac
     3421 aaaaaggaga tgttttaaaa ctaattaata gttttgaacc tgcaccatta attccaattt
     3481 taaacaaaca gggttttgaa gcatttgtag aacacgaaag cagcgaggtt gttaatactt
     3541 atttttataa aataaatgaa acagaaatta acagcccaga tttaacaaac aatgaagagg
     3601 attggcaaag cattattgaa aagtattcta ataatttagt aaccattgat gttcgtaatt
     3661 tagaaatgcc tctaccaatg cttaccattt tagaggagat agaaaaaata ccaaactata
     3721 aagccttata tgtttaccat aaaagaattc ctgttttttt attacccgaa cttaaagaac
     3781 gaaattttga ttatcgtaca aaagaaatca cagatggcga agtacattta ctaattttta
     3841 aagcataaaa aatgttcgtt ggcttaggta attcatccat tgtaaaaacc acttcctata
     3901 aagttgtttt acctttttat ttgtatgcag caatttcatt tattgttgca tcaatatttt
     3961 tattgctttc ttcgaatacc tttttagccc attattttca accacacatt ttagcaataa
     4021 cacatataat ggcattgggt tggggcacaa tgattatttt gggggcaagc catcaattag
     4081 taccagtgtt aatagaagga gagttgtata gttataagtt agcacacctt tcatttatct
     4141 tatctgctat tggtattcca ttattggttt atggttttta tgtttttgat atgggaactc
     4201 ctgcaaaatg gggaggaaga ttagtactac tttccattct gtgttatcta attaatattg
     4261 gttacagtat ttcaaaaagt aagaaagaaa atgttcatgc agtgtatgtt ttcacatcag
     4321 ttatttggtt atttattact gttagtttag ggttagcatt ggtatataat tttacttata
     4381 attttttgcc gcaaaactca ttgcactatt tacctctgca tgtgcatact ggtattttgg
     4441 gctggttctt attgttagtt attggcgtag catccagatt aattcctatg tttttaattt
     4501 caaaatatac aaaccctaca attttatgga ttattttttg gttaattaat accgccctta
     4561 tttcatttat aataatcttc ttttatcttc ctctttctta tacattaatt ccagattatt
     4621 tactttttgt tgccatagtt ttgtttattt attattgtta caatgcctat aaacaaagaa
     4681 taagaaaaca agtagatgag caagtaaaac tatctttact ttcaacagta ttaatgttat
     4741 taccaatact actactattt attatcatta ttttatttat caatagctct gccgaaaatt
     4801 atcaaccaat ggttgcttat ggttttatga tattttttgg atggataaca gccattattt
     4861 taggtatgac ctttaaaaca cttccattta ttgtttggaa taaaatatat catcaacgtt
     4921 ctaataaagg taaaacgcct aaccctaaag atttgtttag caataccatt tttaaaataa
     4981 tgagtatagc ttatatatta ggcattgtaa tttttttatt aggtttagtt tttgaaaatg
     5041 ctattttatt aaaatttggt gcctttttta ttttagtaac ttccatttta tataattgga
     5101 atgttttaaa aattgtttat cacaaaccaa caacaacatg aatttaaaaa ctaataacga
     5161 agaaaaaagt aaacttgcaa tagagggttt gtacaatgta atagatcctg aaataggctt
     5221 aaatgtagtt gaccttggct taatttatga actacatttt gatgaggaag aaaataggct
     5281 aacatgtaca atgaccctaa ctacagagtt ttgcccaatg ggagaatcga taacagataa
     5341 tgcaaggcat gcactgcaac aggcttatgg agattacgga attgatatta atttaatttt
     5401 tgaccctcct tggaatatgg aaatgatttc tgatgaagga agggcatttt taggaagata
     5461 gttatgttaa gtttaagttg taaagctgct attaaagcag taattttttt aggttcaaaa
     5521 tttgaaaccg aagaaaaatt tagtattaaa accattgctg gttttataaa tgaaaatgaa
     5581 cataccgtag gaaaactttt acagaaatta gtaaaagaaa atattatcaa tagcacaaaa
     5641 ggacccaatg gcggtttctt tatttctgct aaacaaatta atcagcctgt aattaaaatt
     5701 gtagaggcta ttgatggaaa agatattttt aaacaatgtg gtttaggatt agaaaaatgc
     5761 tcagaatcgc atccatgccc atttcacaat aactttaaac caataagaga tatgtttaaa
     5821 aaattatgtg aagaaaaaaa agtaatagac ctctatcaaa atgtaaacga aggtttagcc
     5881 tacttaacca actaattatt ttctaagttt tccatttata taataaattt atactcccta
     5941 gaacggtggc ctatatttat gttttaaaca tatagaataa ttatttatta aaaatgaaaa
     6001 ccgaactgtt agagtttgaa aataaacata aattattaga gccaacttca accgaaaggg
     6061 tagctacact agaagcaatt tctgaataca ccgaaaactt tttaggtaat atagatactc
     6121 tgcctgcatt tgtaaacaac gtttcagaaa aagctccatt tatacagagt attaattttg
     6181 aagaacaaaa tactttaaat gaagtattaa aaaatatacg attatatatt gatacaccag
     6241 gtataaatcc tgcctctggc gggcatttag gctatatacc aggtggtggt ttattttatg
     6301 gtgctgttgg cgattactta gcttgcatta caaataggta t
//
LOCUS       DAOQHP010000087         6336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6667_length_6336_cov_3.696068, whole genome shotgun sequence.
ACCESSION   DAOQHP010000087 DAOQHP010000000
VERSION     DAOQHP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6667_length_6336_cov_3.696068"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLY81_04095"
     CDS             complement(<1..318)
                     /locus_tag="PLY81_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29438.1"
                     /translation="MRKIVLIFTIFQVLVVAVSAQSIFTRSTFTAAYSPITVIGGATE
                     GTLTGDDADQLSVPIGFTFSYNGIDRTTINLNTNGTAYFGSTAPTATIGRTNTNLYVT
                     ATNF"
     gene            complement(632..2197)
                     /locus_tag="PLY81_04100"
     CDS             complement(632..2197)
                     /locus_tag="PLY81_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HNF29439.1"
                     /translation="MQPKLFSLLFSTIFISTVIAQPPARTQPPATPASKEATTSSTAN
                     KSGNFPKNILDVDAKVEKNNEVTIKGKSVKYKVTAGTRPIWDKDGEVIAGIFYTFYER
                     TDVDKSTVRPIVFSFNGGPGSASVWMMLGYTGPKLLHIDEEGNPLQPYGLKDNPYSIL
                     DVADIVYVDPVNTGFSRPLGKSVSNETFFGVNADITYLAEWINSFVNKNKRWASPKYL
                     IGESYGTTRVSGLAMELQSRHWMYLNGVILVSPTDLGIKRDGPVADALTLPYYAATAW
                     YHKKLSADLQQKDLVNMLPEVEQFTINEYIPALTKGSQLTDVERNKIIEKVSKYSGLS
                     AKVVKENNLKISTGFFWKELLRDRGFTVGRLDSRYLGLDLKDAGNYPEYNSELVSWLH
                     SFTPAINLYLRDELGYETNLKYNMFGSVYPWNNERNTTGYNLASAMAQNPYLHVLVQS
                     GYYDGACDYFSAQYNMWQLDISNKLKNRISWEGYRSGHMMYLRKEDLAVANEHIRSFI
                     IKSTPAKTMPAKY"
     gene            2344..2571
                     /locus_tag="PLY81_04105"
     CDS             2344..2571
                     /locus_tag="PLY81_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNF29440.1"
                     /translation="MIKLSELAIGQKATIHSFEQNELYIKLMEMGCVPGELVKVEQIA
                     PLGDPISISIAGYSLSLRLSEAEEIFVEEIL"
     gene            2587..3156
                     /gene="nadD"
                     /locus_tag="PLY81_04110"
     CDS             2587..3156
                     /gene="nadD"
                     /locus_tag="PLY81_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNF29441.1"
                     /translation="MNIGLYFGSFNPIHTAHLLIASYVADSALVDKVWFVVSPQNPLK
                     PSNSLLNEYDRLHLVNLAIENDNRFKAVDIEFRLPKPSYTIDTLTYLQEKYPTYTFYV
                     IIGADSYVNLPKWKNYEILKNNYPFIIYNRPGIELKQPLPKNFKILTAPLLEISSTEI
                     RKKIKAKKSILYMLPDAVRIEIERNGYYK"
     gene            complement(3147..4229)
                     /locus_tag="PLY81_04115"
     CDS             complement(3147..4229)
                     /locus_tag="PLY81_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNF29442.1"
                     /translation="MNKHYYVAIMAGGIGSRFWPKSRTSMPKQFLDILNTGKSLIQWT
                     YERFAAFIPEENIFVVTSEDYEDIVAQQLPQLNKQNIVTEPSRKNTAPCIAYISYKLL
                     QKDPKAALIVAPSDHVILDVETFKNTCLKALNFVTQIKAFVTLGITPSYPNTGYGYIQ
                     HDTFSVAEGIYKVKTFTEKPNLELAKTFLASGDFLWNAGIFVWQVNNIIKAFEKFQPE
                     MFDLFENEKALFNSTKEKEAINRIYPLCTNISIDFAIMEKADNVYVMPSSFGWSDLGT
                     WNSAYENLEKDYLANAVVGNNVIIIDATKCMISATDNKLVLLQGLDDCIVVDTQDVLM
                     ICKKDKEQEIKEYVAEVKRIKGDIYL"
     gene            complement(4266..5231)
                     /locus_tag="PLY81_04120"
     CDS             complement(4266..5231)
                     /locus_tag="PLY81_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNF29443.1"
                     /translation="MSNGNIIKIALETIELEGQSVLGLKKFIDSNFENAVNIIHQAKG
                     RVVITGIGKSAIIAQKIAATFNSTGTPSLFMHAADAIHGDLGMLQKEDVAIIISKSGE
                     SPEIKVLVPLIKNFGNTIISIVGNIESFLALQSNIILNTSVSKEACPNNLAPTSSTTA
                     QMVMGDVLAVSLMYLNGFTGKDFAKYHPGGNLGKRLYLRVEDLYKSNQKPQVMEDASL
                     KEVIIEITEKRLGATAVLDNNNHLKGIITDGDLRRMLEKNDQFLNLAAKDIVTTNPKT
                     ISPETMAVQALEVFKTFDITHLIVTENNQYLGILHLHDLLREGIV"
     gene            5397..>6336
                     /locus_tag="PLY81_04125"
     CDS             5397..>6336
                     /locus_tag="PLY81_04125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNF29444.1"
                     /translation="MPISRKAATKKTTTAKKTIKRPVLENNRLPKKKYEFDLQEGLQK
                     YFGFTEFKGTQEKAIKSLLAGDDTFVIMPTGGGKSLCYQLPAMLMPGCAIIVSPLIAL
                     MKNQVDLVRGYSEQDDVAHFLNSSLNKGQIKIVKDDLLSGATKLLFVAPETLTKEENL
                     DFFSDLNISFFAVDEAHCISEWGHDFRPEYRRLREMMDVINPHIPVIALTATATPKVQ
                     SDIIKNLGLREPNIFISSFNRTNLYYEIQPKGKKEQTVKHIVKFIASHKGKSGIIYTL
                     NRKTTEELAELLVANNIKAVAYHAGLDQKVRAKGQDQ"
BASE COUNT         2141 a         1017 c          957 g         2221 t
ORIGIN      
        1 aaaatttgtg gcagttacat acaaattagt atttgttcta ccgatggttg cagttggtgc
       61 tgtgctgcca aaatatgctg ttccgttagt gtttaaatta atggtagtac gatctatacc
      121 attatagctg aatgtaaaac caatgggtac agaaagctgg tctgcatcat cgccagttaa
      181 tgtaccttct gtagcaccac caataacagt tattggagag taagctgcag taaatgtgct
      241 tcttgtaaat attgattgtg cagatacagc aacaaccaat acttggaaaa ttgtaaaaat
      301 gagtacaatc tttctcataa ttgatagatt taggtaaatt aaatagggtt taaatgaaga
      361 atagcgggtt ttaaacttta gaccaagttt atcagtagta tgatggattt gtgaattgca
      421 atttatatta tattttcttt aacaaaaaat ttttttttgt tagaatatat ttcaatatcc
      481 aattcagata aggagatttt aatatttaat taaatgctgc attagagtat aaattttttt
      541 tatttttttg aactagataa tttgtttaat aaaaaagcca atgcaaataa tgtcattggc
      601 tataaaaaaa ctgattgtaa aaataaattt attaatactt tgcaggcata gttttagctg
      661 gagtagattt aataataaat gaccttatat gttcgtttgc aactgccaaa tcttcttttc
      721 ttaaatacat catgtggcca cttctatacc cctcccaaga aattctattt ttaagtttat
      781 tagaaatatc taattgccac atattatatt gggcactaaa ataatcgcaa gccccatcgt
      841 aataaccact ttgcactaaa acatgtaaat atgggttttg ggccatagca cttgcaaggt
      901 tataacctgt agtatttctc tcattattcc aaggatatac cgagccaaac atattgtatt
      961 ttaaattagt ttcatagcct aattcatctc ttaaatatag gttaatggca ggtgtaaaac
     1021 tatgtaacca agaaactaat tcggagttgt attcagggta atttcctgca tcttttaaat
     1081 ctaatcctaa gtatctagag tctaaccttc ctacagtaaa acctctatct cttaataatt
     1141 ctttccaaaa aaagcctgta gaaattttta aattattttc ttttaccact ttggcagata
     1201 agcctgagta tttactcact ttttctatta ttttgtttct ttctacatct gtaagttggc
     1261 tgcctttggt taatgcaggt atatattcgt taatagtaaa ttgttctact tctggcaaca
     1321 tatttactaa atctttttgt tgtaaatctg ccgatagttt tttatggtac catgcagtag
     1381 cagcataata aggaagggtt aaagcatctg caactggtcc atctctttta atacctaaat
     1441 cggttggaga aactaaaatt actccgttta aatacatcca atgtctgctt tgtaattcca
     1501 tagcaagccc agaaactctt gttgtgccat agctttcgcc aattaaatat tttggcgaag
     1561 cccatctttt atttttattt acaaagctat ttatccattc tgccaggtag gtaatatctg
     1621 catttacacc aaagaaagtt tcgttactaa ctgattttcc taatggcctt gaaaagcctg
     1681 tatttacagg atctacataa acaatatcgg caacatctaa aatagaatac gggttatctt
     1741 ttaagccata aggttgtaaa ggatttcctt cctcatcaat atgtaaaagc tttggccctg
     1801 tatagccaag catcatccat acacttgcac ttcctgggcc accattaaat gaaaaaacta
     1861 tgggacgtac agtacttttg tccacatcgg ttctttcata aaaagtataa aaaataccgg
     1921 caattacttc tccatcttta tcccaaatag gtcttgtacc agcagttacc ttatatttta
     1981 ctgatttgcc tttaatagtt acctcattat ttttttcaac ttttgcatct acatctaaaa
     2041 tatttttagg aaaattaccc gacttgtttg cagttgaaga tgttgtagcc tctttagatg
     2101 caggtgttgc aggtggctgt gttcttgctg gtggttgagc aattacagta cttatgaata
     2161 ttgtactgaa caataaacta aataattttg gttgcatatt ggttatattt tttcttaaaa
     2221 tgaaaggtat aatttttagt taagaaagat gaaaatgatt gtatgagtgg tagaaaaaag
     2281 attaatctta aatcttcatc actactggta tataactaca tttgcagcaa taaaaattgt
     2341 ggaatgatta aattgagcga attagcaata ggccaaaaag caaccattca ttcttttgaa
     2401 caaaatgagt tgtatattaa attaatggaa atgggttgtg tacctggaga gttagttaaa
     2461 gtagagcaaa ttgcaccact tggcgatcct atttctatca gtattgctgg ctactcttta
     2521 agtttaaggc ttagtgaagc agaagaaatt tttgtggaag aaatactata actgaattta
     2581 tctacaatga atattggatt gtattttggc tcttttaacc ccattcatac tgcccattta
     2641 ttaattgcaa gttatgtggc agatagtgct ttagtggata aggtttggtt tgttgtttct
     2701 ccgcaaaacc cattaaaacc ttctaatagt ttattaaatg aatatgacag gttacattta
     2761 gtgaatttag ctatagaaaa tgataacagg tttaaagctg ttgatattga atttagatta
     2821 cccaaaccat cttatacaat tgatacttta acttacttac aagagaaata tcctacttat
     2881 actttttatg taattattgg tgcagatagt tatgtaaact tgcccaaatg gaagaattac
     2941 gaaatactaa aaaataacta tccttttatt atttataata gacctggcat tgagttaaaa
     3001 caaccattac caaaaaattt taaaatactt actgctccac tgttagagat ttcctcaacc
     3061 gaaataagaa aaaaaataaa agctaaaaaa agtattttat atatgttgcc cgatgctgta
     3121 agaatagaaa tagaaaggaa tggttactat aaataaatat ctccttttat tctttttacc
     3181 tctgcaacat attctttaat ttcttgttct ttatcttttt tgcaaatcat taatacatct
     3241 tgagtatcta ctacaataca atcatctaaa ccttgcaata gcactaattt attatccgta
     3301 gcactaatca tacatttggt agcatcaata ataattacat tattgcctac tactgcattg
     3361 gctaaataat ctttttctaa attttcataa gcactgttcc aggttcctaa atcactccag
     3421 ccaaatgagg aaggcataac ataaacgtta tctgcttttt ccataatggc aaaatctata
     3481 gaaatattgg tacataaagg atagatacga ttaattgctt ctttttcttt tgtagaattg
     3541 aaaagtgctt tttcattttc aaaaaggtca aacatttctg gttgaaattt ttcgaatgcc
     3601 ttaataatat tgtttacctg ccaaacaaat atgcctgcat tccataaaaa atctccactt
     3661 gctaaaaagg tttttgctaa ttctaaattt ggcttttcgg taaaagtttt tactttataa
     3721 ataccttcgg caacactaaa agtatcgtgt tgtatataac catagcctgt atttgggtaa
     3781 gatggagtta tgcctaaagt aacaaaagct ttaatttgtg taacaaaatt taatgctttt
     3841 aaacaagtat ttttaaaggt ttctacatct aaaattacat gatcgctggg tgctacaatt
     3901 aatgcagctt tggggtcttt ttgtaaaagt ttataagata tataagcaat acaaggggca
     3961 gtattttttc tgcttggttc tgtaacaata ttttgcttat ttaattgtgg caattgttga
     4021 gcaacaatgt cttcataatc ttcggaagta acaacaaaaa tattttcttc gggaataaat
     4081 gctgcaaacc tttcataggt ccattgaatt aagcttttgc ctgtatttaa aatatctaag
     4141 aattgtttag gcatagaggt tctgctttta ggccaaaacc tactaccaat tccgccagcc
     4201 attattgcaa cgtaataatg tttattcata tagcaaaaaa taagaagtgc aaataaacgt
     4261 atattttaaa caatcccttc tcttaataaa tcatgtaaat gtaaaatacc taaatattgg
     4321 ttattttctg taacaattaa atgggtaata tcaaaagttt taaatacctc taaagcttgc
     4381 acagccattg tttcgggaga tattgttttt gggtttgtag ttacaatatc ttttgcagct
     4441 aaatttaaga attggtcatt cttttctaac attcttctca aatctccatc tgtaataatg
     4501 ccttttaaat ggttattatt atctaacacg gcagtggcac ctaatctttt ttcggttatt
     4561 tctataatca cctcttttaa agaggcatct tccataacct gtggtttttg attgctttta
     4621 tacaaatctt ccactcttaa atataatctt ttacctaagt ttccgcctgg gtgatattta
     4681 gcaaaatctt ttcctgtaaa tccgtttaag tacattaaag aaacagccaa tacatctccc
     4741 attaccattt gtgctgtagt actacttgtg ggagccaaat tattagggca agcctcttta
     4801 ctaacagaag tatttaatat aatattactc tgtaatgcaa gaaaactttc tatattacca
     4861 acaattgaaa ttatagtatt accaaaattt ttaattagcg gaaccagtac ttttatttcg
     4921 gggctttcgc cacttttact aataataatg gcaacatctt ctttttgtaa catacccaaa
     4981 tcgccatgta tagcatcggc tgcatgcata aataaagaag gtgtgccagt agaattaaaa
     5041 gtagctgcaa ttttttgagc tataatagca cttttgccaa tgcctgtaat aactaccctg
     5101 ccttttgcct gatgaattat attaactgca ttttcaaaat ttgaatcaat aaattttttt
     5161 aagcctaaca ccgattgacc ttctagttca atggtttcta aagctatttt tatgatattt
     5221 ccgttactca tttgtcaaag gtaaagttta acaacttact gttgtaattc aatactaatt
     5281 tcattaactt cgctaccctt taaaaattta ctataaattt ttatttctgt aataagaata
     5341 aattgtatct tttaaccttt aaagatttat agataaagag gaaaagaaca cttattatgc
     5401 ccatatcaag aaaagcagct accaagaaaa ccacaactgc aaaaaaaact attaagcgac
     5461 cagttttgga aaacaatcgt ttacccaaaa aaaagtatga gtttgactta caggaagggt
     5521 tacaaaaata ttttggcttt acagagttta aaggaacgca agaaaaagca attaaaagct
     5581 tacttgcagg cgatgatact tttgtaatta tgccaacagg tggtggcaaa agtttatgct
     5641 atcaactgcc agcaatgctt atgcctggtt gtgctattat tgtatcgcct cttattgcat
     5701 taatgaaaaa ccaggtagac ttagttagag gctatagtga acaagacgat gtagcacatt
     5761 ttttaaactc ctccttaaat aaagggcaaa taaaaattgt aaaagatgat ttactttctg
     5821 gagcaaccaa gttacttttt gttgcaccag aaacattaac aaaagaagaa aaccttgatt
     5881 tttttagcga cctgaatatt tctttttttg cagtagatga agcacactgt ataagtgaat
     5941 gggggcatga ttttagacca gaatacagaa ggttgagaga aatgatggat gtaattaatc
     6001 ctcatattcc tgtaattgct ttaactgcaa ctgcaacacc taaagtgcaa agtgatatta
     6061 taaaaaactt aggattaaga gaacccaata tatttatttc atctttcaat cgtacaaatt
     6121 tatattacga aattcagcct aaaggaaaaa aagaacaaac tgtaaaacac attgtaaaat
     6181 tcatcgcctc acataaaggt aaaagcggaa ttatttacac tttaaataga aaaacaaccg
     6241 aagaattagc agaactttta gttgccaata atattaaagc tgttgcatac catgctggct
     6301 tagaccaaaa agtgagagct aaaggacaag atcagt
//
LOCUS       DAOQHP010000088         6326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6684_length_6326_cov_3.459895, whole genome shotgun sequence.
ACCESSION   DAOQHP010000088 DAOQHP010000000
VERSION     DAOQHP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_6326_cov_3.459895"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLY81_04130"
     CDS             complement(<1..235)
                     /locus_tag="PLY81_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNF29445.1"
                     /translation="MALPKEPRQKMINLMYLVLTALLALNVSSEILNAFKTVKSSLES
                     SSELANGKTQQIFESFKDKMEDPTTKEQATIWYE"
     gene            complement(317..1084)
                     /gene="gldL"
                     /locus_tag="PLY81_04135"
     CDS             complement(317..1084)
                     /gene="gldL"
                     /locus_tag="PLY81_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNF29446.1"
                     /translation="MAAKIPPKVGKIVDVLVSIAAAIVLFGALQKLMHTKLADLFLQI
                     GLYSEAAIFLGYGILYMYYPAMDEHEVHVPGLEGVEKDNNPLKAMEKMMKEADITPAN
                     LGKLSAGFEKFGSTVSQISDLGDVVKSTSDFSSKTKEAATALGSVAGAVSQATVSLGS
                     FNEASESTKQFHGQVQVLTKNLASLNTIYELELQESNNHLKALNQFYGKLAEAGAAMN
                     GTAEDAEKAKQQIAALAGNLSKLNQVYGNMLTAMAGR"
     gene            complement(1194..2492)
                     /locus_tag="PLY81_04140"
     CDS             complement(1194..2492)
                     /locus_tag="PLY81_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF29447.1"
                     /translation="MRNSLSALLLPALLISLVACNKKGKVKGTPDNGQLVGVAPSAKP
                     NTGRLAGLVYIPPGTFHMGPSDEDINFNYTARNKQVSINGFWMDNTEITNNEYRQFVY
                     WTRDSIALKELGYVKNTNGGGGGDSSIAVDWSKKKDIKYDQSTLEKLGNKLMIDAPER
                     LYGKIEVDPKKIVYHIEYFDLTEAARIENKDIPRTNFIKRFDQQIYPDTLVWLRDFSY
                     AYNEPMTKRYFSHPSFGNYPVVGVNWKQAVAFSHWRTHYLNSYLGKKKKATESDFRLP
                     SEAEWEYAARGGRTNSMYPWGNYYLRNKKGCLLANFKPGRGNYPEDGGFYTVRADAYW
                     PNDFGLYNMSGNVAEWTGSYYYEGAYNLIHDMNPDIRYDAKDSDPPRMKRKVVRGGSW
                     KDVGYYLQTGTRSYEYQDTAKSYIGFRCVIDLAPRSGKRK"
     gene            complement(2700..4730)
                     /locus_tag="PLY81_04145"
     CDS             complement(2700..4730)
                     /locus_tag="PLY81_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF29448.1"
                     /translation="MYTNKLISETSVYLLQHAHNPVNWFTWGEEAFKLAKEENKPILL
                     SIGYAACHWCHVMEKESFEDVETASLMNKYFINIKIDREERPDIDAIYMDAVQAITGS
                     GGWPLHVFLTPDKKPFYGGTYFPPERKYNRLSWKEVLTNIADAYTNKYNEIEEQANKL
                     TQHLQQSNLLNFQNLNLEEEQIFNEETDFEIASTILKSADMVDGGFGNAPKFLQTYSI
                     QYLLRHYHFTKNIDALNHAVLSLNKMISGGIYDQLGGGFSRYSTDAQWHAPHFEKMLY
                     DNALMVNLLSEAYQLTNNKDYKRIIEETLEFVLSEMTAKNEGFYSAFDADSEGEEGKF
                     YTWTKTEIENALKEKAKLFCSYYNITERGNWENTNILFAKHSIAEFAQKQNITENELS
                     IFLIECKKILFEIRVNRIKPNLDDKVLLGWNALMITAFCKAFAATGNLKYKQTAIEKI
                     YFIENNLKVNSQWKHTYKNGKSKIDAFLDDYAYLIEAYIYLQEISADSKYLIIAKQLT
                     QHVLKNYSSNSIFFNYTNQNQPDILVHKQDVYDGALPSANAIMAKNLDYLGIVFSIQE
                     WQNIANKMLQNMVPSIINHPNSFAVWASLVQQKIYGTNEIALCGKSILTELPKLLQKY
                     IPNKVLQTSIVEKKDFPLLKNRFLIEKNTFYLCRNNSCYKPFDTIEALLAIV"
     gene            complement(4828..5622)
                     /locus_tag="PLY81_04150"
     CDS             complement(4828..5622)
                     /locus_tag="PLY81_04150"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNF29449.1"
                     /translation="MAKIGAKSTVPTKAVKRILISQPRPESEKSPYFDLERKYDVQLF
                     FTPFIKLDAITAKEFRKQKIDIASYSAVVFTSRNAIDFFFKMCEEMKVPVSQDTKYFC
                     ITEAVALYLQKFTLYRKRKVFYGADGSNKSLFDVINKHKENERFMYVCSENQQDNEIV
                     NWFKNHHCDFTLAFMYRTISNDIKEFFKKTSYDVICFFTPSGIKSLFDNIPEFKQKNT
                     EIGVFGNNTLLAAENAGLKTLIKAPIPQVPSMITALDKYLAEKNKN"
     gene            complement(5643..>6326)
                     /locus_tag="PLY81_04155"
     CDS             complement(5643..>6326)
                     /locus_tag="PLY81_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNF29450.1"
                     /translation="IFMIDVKRKAENNHFLFYLLIGTLLFASLIKAIFSKYFSDIFKI
                     FFQSSFRQKQTREQLLQDSLPSLLMNILFFISSSIFITLIAKQYHLLIENFWLTLLYS
                     TILITSVYFFKYIFLSFIGWVFNIAKTINAYVFTVFIINKLLGVILIPVILIMAFSLH
                     NIAYVSLIIALILIAILFIYRYWLGIKSVRNSFKVSALHFFLYLCAVEVLPMVLLYKA
                     VFNVFKSNL"
BASE COUNT         2028 a         1039 c          871 g         2388 t
ORIGIN      
        1 tttcgtacca aatagtagct tgctctttgg tagttgggtc ttccatttta tctttaaaag
       61 attcaaatat ttgctgagtt ttgccattag ctaactcact tgaactttct aagctacttt
      121 ttacagtttt aaaagcgttt aatatttcag atgatacgtt taatgctaat agtgcagtta
      181 acactaagta cattaagtta atcatcttct gccgtggctc cttaggtagt gccatgttgt
      241 attattttat ttaattcaat ttacaaatta ggtttatctc gtttaatttc ttgtatttat
      301 cgtaatacaa tagaaattat ctaccagcca ttgcagttag catattacca taaacttggt
      361 taagtttact taaattacca gctaatgcag caatttgttg tttagctttt tccgcatctt
      421 ctgctgtacc attcattgca gcacctgctt cagctaattt accatagaat tggtttaaag
      481 cttttaagtg gttattactt tcttgtaatt ccaattcata aattgtattt aatgatgcta
      541 agtttttagt taaaacttga acttggccat gaaattgttt agtactttca gaagcctcat
      601 taaatgaacc aagagaaaca gttgcttggc taacagcacc agctacactt cctaatgcag
      661 ttgcagcttc tttagtttta cttgaaaaat cagaagttga tttaacaaca tctcctaaat
      721 cgcttatttg tgaaactgta gaaccaaatt tttcaaaacc agcacttaat ttacctaaat
      781 tagctggtgt aatatctgct tctttcatca ttttttccat tgcttttaat ggattattat
      841 ctttttctac accctccaaa cctggcacat gcacttcgtg ttcgtccatt gcaggatagt
      901 acatgtatag aataccataa ccaagaaata ttgcagcctc tgaatacaaa cctatttgaa
      961 gaaataagtc tgctaattta gtatgcatta atttttgtaa agctccaaat aatactattg
     1021 cagctgcaat agatactaat acgtcaacga ttttaccaac tttaggagga atttttgctg
     1081 ccatgtttta gatttttaaa tttttgtggt tttatattta ctgaataaat agactttttt
     1141 ttattgtaca taaggttgta caacttcttt attaattaat tcttattatt agtttatttt
     1201 cttttacctg atcttggtgc taggtcaatt acacaacgga accctatata tgattttgct
     1261 gtatcttgat attcatatga tcttgttccg gtttgtaagt aataacctac atctttccaa
     1321 ctgccccctc taacaacttt tcttttcatt cttggagggt cgctatcttt tgcatcgtaa
     1381 cgaatatctg ggttcatgtc atgaattagg ttgtatgcac cttcataata gtatgaacct
     1441 gtccattctg caacattacc agacatatta tataatccaa aatcatttgg ccaatatgca
     1501 tcagctcgta ctgtgtaaaa ccctccatct tctgggtaat taccacgacc tggtttaaag
     1561 ttagctaata aacatccttt tttatttctt aaatagtagt taccccaagg atacatagag
     1621 tttgttctgc ctccacgagc agcatactcc cactcagctt cacttggtaa tctaaaatca
     1681 ctttcagttg cctttttctt tttacctaaa tagctattca ggtaatgtgt tctccaatga
     1741 gagaaagcta ctgcttgttt ccagttaaca cctactacag gataattacc aaatgatggg
     1801 tgactgaaat atcttttagt cattggttca ttataggcat aagagaaatc tcttaaccaa
     1861 accaatgtat ctggataaat ttgttggtca aatcttttta taaaattagt tctaggaatg
     1921 tctttatttt caattctagc agcctctgtt aaatcaaaat attcaatatg gtaaactatt
     1981 tttttaggat ctacttcaat ttttccatat aatctttcag gagcatctat cattagttta
     2041 tttcctaatt tttcaagagt gctttgatca tatttaatat cctttttctt gctccaatca
     2101 acagcaatag aagagtctcc tcctccacca ccattggtgt ttttaacgta gcctaactct
     2161 tttaatgcaa tggaatctct tgtccaataa acaaactgac gatattcgtt attagtaatt
     2221 tcagtattat ccatccaaaa accattgata gatacttgtt tattacgtgc agtatagttg
     2281 aagtttatgt cttcatcgct tggccccata tgaaaagttc ctggtggtat gtaaaccaaa
     2341 ccagcaagcc ttccagtatt gggctttgca cttggtgcaa cgccaactaa ctgaccatta
     2401 tcaggtgttc ctttcacctt accttttttg ttacaggcta ctaaacttat cagtaaagca
     2461 ggtaacagga gagcacttaa actatttctc attctacaaa ataattgaag ggtagtacag
     2521 tgtacaaata tatagatatt ttttttgtcg caaatttttt ttcataaaac aaccctatta
     2581 agggcattta ataacgattg attttaaaac ttattgtatt atataaatgt attttggtag
     2641 aattttaacc tatagatagt ttttatgtat ttcaaggtct aaattaagcc aacacattgt
     2701 tatacaattg ctaaaagtgc ttcaatagta tcaaatggtt tgtaacaact gttatttcgg
     2761 cacaaataaa aggtattttt ttctattaaa aacctatttt ttaataatgg aaaatctttt
     2821 ttttccacaa tagaggtctg taaaacttta tttggaatat atttttgaag taatttgggt
     2881 aattcggtta aaatgctttt accacaaagt gctatttcgt ttgtgccata aattttttgt
     2941 tgtactaaag atgcccaaac tgcaaatgaa tttgggtggt ttataatgga tggaaccata
     3001 ttttgaagca ttttattggc tatgttttgc cattcctgaa ttgaaaaaac aattcctaaa
     3061 taatctaagt tttttgccat tattgcattt gcacttggta atgcaccatc gtacacatct
     3121 tgcttatgca ctaaaatatc tggctgattt tggttggtat aattaaaaaa tattgaattg
     3181 ctactataat tttttaaaac atgttgggtt aactgtttgg caattattaa atatttacta
     3241 tcagccgata tttcttgtaa ataaatataa gcttcaatta aataagcata atcatctaaa
     3301 aaagcatcaa tttttgattt gccattctta taagtatgtt tccattgact gttaaccttt
     3361 aaattatttt caataaaata tattttttct atggctgttt gcttgtattt aaggttgcct
     3421 gttgctgcaa atgctttaca aaatgcagtt atcattaatg cattccaacc cagtagaacc
     3481 ttatcgtcta aatttggttt tatcctattt acccttattt caaataatat ttttttacat
     3541 tctattaaaa aaatacttag ttcattttca gtaatatttt gtttttgtgc aaattctgca
     3601 atagagtgtt ttgcaaataa tatattagtg ttctcccagt tacctctctc ggttatatta
     3661 taataggaac aaaaaagctt ggctttttct tttaaagcat tttcaatttc ggttttagtc
     3721 caagtataaa acttaccttc ttctccttca ctatcagcat caaaagcact ataaaaccct
     3781 tcatttttgg ctgtcatttc ggataataca aactctaagg tttcctctat aattctttta
     3841 taatctttat tatttgttaa ttgatatgct tcggataata gatttaccat tagagcatta
     3901 tcgtataaca ttttttcgaa atgaggtgca tgccactgtg catctgtact gtatcttgaa
     3961 aagcctccac ccaattgatc ataaatacct cccgatatca ttttatttag gcttaaaaca
     4021 gcatggttta aggcatcaat gttttttgta aaatgatagt gccttaacaa atattgtata
     4081 gaataagttt gaagaaattt aggagcattt ccaaaaccac catcaaccat atcggcagat
     4141 tttaaaatgg ttgaggctat ttcaaagtct gtttcttcat taaaaatttg ctcttcttct
     4201 aaatttagat tctgaaaatt gagaagatta gattgttgta agtgctgtgt aagtttattt
     4261 gcttgttcct ctatttcatt atacttatta gtataagcat ctgcaatatt agtaagtact
     4321 tctttccagc ttaatctatt atactttctt tcaggaggga aataagtacc tccataaaat
     4381 ggcttcttat ctggcgttaa aaatacatga agtggccaac ctccgcttcc tgtaattgct
     4441 tgcactgcat ccatataaat tgcatcaata tctggtcttt cttctctatc tattttaatg
     4501 tttataaaat atttgttcat taaacttgca gtttctacat cttcaaagct ttctttctcc
     4561 attacatggc accaatgaca agctgcataa ccaatgctaa gtaatatggg cttattttct
     4621 tcttttgcca atttaaaagc ctcttcgccc caagtaaacc agttaacagg gttgtgggca
     4681 tgttgtagaa gataaacgga ggtttcacta attaatttat tagtgtacat aataatatac
     4741 tttaaggaaa taaagttacc tgtttgctaa aagaataaaa gcaatgatta gtgtttctac
     4801 aaaaaatacc ttaagtagta aagtgtatta atttttattt ttttcagcta aatatttatc
     4861 taatgcagta atcatactag gcacttgtgg tattggtgct ttaattaaag ttttaagacc
     4921 ggcattttcg gcagctaata aagtgttatt tccaaaaacc ccaatttcag tatttttttg
     4981 tttaaactca ggtatattgt caaataaact ttttatacca cttggtgtaa aaaagcaaat
     5041 tacatcataa cttgtttttt taaaaaactc tttaatatcg ttgctaatgg ttctatacat
     5101 aaaggctaaa gtaaaatcac aatgatggtt tttaaaccag tttacaatct cattatcttg
     5161 ttgattttcg ctacacacat acataaatct ttcattttct ttatgtttgt ttatcacatc
     5221 aaataaactt ttattggatc cgtctgcacc ataaaaaact tttctttttc tatataaggt
     5281 aaacttttga agatataaag ctacagcctc agtaatacag aaatatttag tatcttgact
     5341 aactggtact ttcatttctt cgcacatttt aaaaaagaag tcaatggcat ttctactggt
     5401 aaaaactaca gcagaatacg aagcaatatc tattttttgt ttacgaaatt ctttagcagt
     5461 aattgcatct aatttaataa atggagtaaa aaataattgc acatcgtatt ttctctccaa
     5521 atcaaaataa ggagattttt cactttcggg acgaggctga gaaattaaaa ttctctttac
     5581 tgcttttgtt ggtactgtac tttttgctcc tattttagcc atgcttttct acaaaattta
     5641 tgctataaat tactcttaaa tacattaaag acagccttat acaataacac cataggcaat
     5701 acttcaacgg cacaaaggta aagaaaaaaa tgtaaggcac ttactttaaa actatttcgt
     5761 acagatttta ttcctaacca atacctatag atgaaaagta tagctattaa tattaaagca
     5821 atgataaggc ttacgtaagc aatattgtgc aaagagaaag ccattattaa aataactgga
     5881 attagaatta cgcctaataa tttattaatt ataaaaacgg taaatacata ggcattaatg
     5941 gttttggcaa tgttaaatac ccatccaata aaacttaaaa aaatgtattt aaaaaagtaa
     6001 acggatgtaa ttaaaatggt gctatataat aatgttaacc aaaaattttc tatcagtaaa
     6061 tggtattgct ttgcaattaa agttataaaa atactactgc ttataaaaaa tagaatattc
     6121 attaatagag atggtaaact atcttgcagt aattgctctc tagtttgttt ttgcctaaaa
     6181 gatgattgaa aaaagatttt aaatatatct gaaaaatatt tcgagaatat tgctttaatt
     6241 aaggaagcaa aaagtaaagt accaattagt aaataaaata aaaagtggtt attttctgct
     6301 tttcttttta catcaatcat aaatat
//
LOCUS       DAOQHP010000089         6261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6797_length_6261_cov_3.478086, whole genome shotgun sequence.
ACCESSION   DAOQHP010000089 DAOQHP010000000
VERSION     DAOQHP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_6261_cov_3.478086"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..599)
                     /gene="rpsG"
                     /locus_tag="PLY81_04160"
     CDS             complement(132..599)
                     /gene="rpsG"
                     /locus_tag="PLY81_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNF29451.1"
                     /translation="MRKAQAKKLPLAPDARFNDKQVTRFINNLMWDGKKSTAITIFND
                     AVDKVSSITGEDGYEVWKKALANVTPAVEVRSRRIGGATFQIPSEVRADRKISLSMKW
                     LIRYSRERNGKTMADKLASEIISASKGEGAAFKKKEDVHRMAEANKAFSHFKV"
     gene            complement(681..1061)
                     /gene="rpsL"
                     /locus_tag="PLY81_04165"
     CDS             complement(681..1061)
                     /gene="rpsL"
                     /locus_tag="PLY81_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNF29452.1"
                     /translation="MPTIQQLVRKGREIIRAKSKSRALDACPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVKDRKQSRSKYGTKKEKAKK"
     gene            complement(1249..2313)
                     /locus_tag="PLY81_04170"
     CDS             complement(1249..2313)
                     /locus_tag="PLY81_04170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNF29453.1"
                     /translation="MTAAVDINVIKAESSKLKEMSLENIPFGKYFTDHMLEADYENGE
                     WKNVEIKPYQPLVIEPSMASLHYGQAIFEGIKAYKSPNGDAFIFRPKDNYNRFNVSAE
                     RMQMPAVPEDIFMEGMRMLVELDKSWIPGHTDHSLYIRPFMFSTDHLIGVRPSETYKF
                     MIILSPTGPYYNAPMKIYVEEKYTRAAPGGVGFSKNAGNYAASMYPTAMARKAGYDQV
                     LWTDAFEHKYLQEVGTMNVFFIIGNKAITPSLEDGTILAGVTRDSAITILKKMGLEVV
                     ERKITTDELIDVYKSGELKEVFGTGTAATISLIKELKYKDFIMEFDVDSWKVSTELKE
                     RLNGIRYGTATDEFGWMYKL"
     gene            2385..2897
                     /locus_tag="PLY81_04175"
     CDS             2385..2897
                     /locus_tag="PLY81_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29454.1"
                     /translation="MNEPISLSKYVIAKLYKDNIVITDKIEENLEPEKSADVNILFLG
                     ENKKNIVILLKEEDAMHLKDDSFQFLSSILQACKLNMADVAIINYNNQSINLNKVTKQ
                     FAPKFVIIFGELPNTIKQDATIDFYTPININSAVWLKAAVLDEMKENSTNAKVEKSKL
                     WNCLKQIFKL"
     gene            2901..3503
                     /gene="rdgB"
                     /locus_tag="PLY81_04180"
     CDS             2901..3503
                     /gene="rdgB"
                     /locus_tag="PLY81_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNF29455.1"
                     /translation="MTEKFTLIFATNNANKVQEIKSVLGNNFEIISLKEAGIDIEIPE
                     PYDTLKENATEKSSVIFRLTNKNCFSEDTGLEVEALHGEPGVKSARYANEYPKFESNI
                     EKLLYNLKDIKNRNAQFRTVISLIINKQEYFFEGICKGKIIDTPKGNEGFGYDPIFVP
                     DGSNKTFAEMPIEEKNKFSHRKKAMQQLILFLSQNKTNFS"
     gene            3607..3891
                     /locus_tag="PLY81_04185"
     CDS             3607..3891
                     /locus_tag="PLY81_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29456.1"
                     /translation="MASSAIAFNNDLLKENWRVKPSSSTITKEAKVSYLMVEQVEPPK
                     YMWDKICKILDAEKEAENKTNYQKSSNKTIKALLIAGGLITIASILLFLL"
     gene            3972..4406
                     /locus_tag="PLY81_04190"
     CDS             3972..4406
                     /locus_tag="PLY81_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF29457.1"
                     /translation="MQRVKVELPINFNFSTIIPIRITDMNYGGHVGNDSFLSLIHEAR
                     MQYLQHFGYTEINFENTSLIMADAAIEFKHEIIYTNTIKISVQAAAFTKIGFDLYYLL
                     EIINGEELIIAGKAKTGMLCFDYTNKKITTVPTKVISKLTTQ"
     gene            4417..5445
                     /gene="thiL"
                     /locus_tag="PLY81_04195"
     CDS             4417..5445
                     /gene="thiL"
                     /locus_tag="PLY81_04195"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNF29458.1"
                     /translation="MQQRTEIANLGEFGLIEHLTKNFEIKNASTILSVGDDAAVIDHF
                     GKQTVITTDMLVEGIHFDLMYTPLKHLGYKSVIVNLSDVYAMNAIPTQITISIAFSNR
                     FSVEALSEFYEGVYAACEKYAVDLVGGDTTSSQKGFIISVTAIGEVAPDKYVQRSTAQ
                     QGDLICVSGNTGGAFLGLTLMQREKKIYLENPAIQPDLEGEDYIVGRLLKPEARKDII
                     EFFAANNIVPTAMMDVSDGISSEVLHLCKQSNVGCKIFEDKLPIDEKTRAAAFKFGLD
                     PTVCALNGGEDYELIFTLKQEDYDKITLNEEIAVIGYMTALEEGNKLQTKGGNIFDIT
                     AQGWNAFT"
     gene            5470..>6261
                     /locus_tag="PLY81_04200"
     CDS             5470..>6261
                     /locus_tag="PLY81_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29459.1"
                     /translation="MKKKLIHIYLDSIVILFSLLTSCVSYKIPNYTYNKKLPVALLKE
                     DLTILKKTLEANHPSLYWYNTKDSIDFNFENALSTMKDSLNEVQFKKKVAEIISTIHC
                     GHTSVNYSKNFTKLYKHHLYPMFPLYLKVWADSMVVLGNTIETDSILKRGVIITTING
                     VSNRLILDSIFKSISTDGYSVNYKNQVVSNNFPAWYKQTWGLDTSYTISYLDSLGNKA
                     TTTIKNYSPIKDTSTIKKEITKIKKPNRKQLKNARRLAKRYLQIDT"
BASE COUNT         2248 a          967 c          905 g         2141 t
ORIGIN      
        1 caacattcct gagaaacaaa aagactcagg ttgaatttga accagggtct gatacgtatc
       61 agaccttttt ttatgcaaaa aaaatggcta caaaatttgt aaccattact gtataataaa
      121 aaataagtaa attaaacttt aaagtgagag aatgctttat ttgcttctgc catacgatgt
      181 acatcttctt tctttttaaa tgcagcacct tctcctttac ttgcagatat aatttcactg
      241 gctaatttat ctgccattgt tttaccattt ctttcacggc tataacgaat taaccatttc
      301 atgcttaaag aaatttttct atctgcacga acttcacttg gtatttgaaa agtagcacca
      361 cctattcttc tgctacgtac ttcaacagct ggtgtaacat tggctaatgc ttttttccat
      421 acttcataac catcttctcc tgttatgctg ctaactttat ctacagcatc attaaaaatg
      481 gtaatggctg tgcttttttt accatcccac attaaattgt taataaaacg agttacctgt
      541 ttgtcgttaa atctagcatc tggtgctaaa ggcaactttt tggcttgtgc tttacgcatt
      601 ggttttaatt tttaagttta caaactgaat ttcttcaagt attaaaactt tactcagttt
      661 ataataatta ttgaaattga ttatttttta gctttttctt tctttgtacc atatttacta
      721 cggctttgtt ttctatcttt tacacctgca gtatctaaac taccacgtac aatatgataa
      781 cgtacacctg gtaaatcttt tacccttcca ccacgtatta aaactattga gtgctcttgc
      841 aaattatgac cttctccagg tatataagca attacttcaa ttttatttgt taaacgaact
      901 ttggcaactt tacgtaaagc tgagttaggt tttttaggtg tagtggtata tactcttgta
      961 caaacaccac gacgttgagg acaagcatct aaagctcttg atttgctttt tgctctaata
     1021 atttcacgac cttttcttac taattgttga attgtaggca ttgtaacctt ttatttttta
     1081 ggacggcaaa agtagtagtt tttgcttata atccaaaaga tttaataaaa tattattgtt
     1141 tttttttcac atttaatgta aaaaatttat tggaaaacag atttataccc atttattcac
     1201 aaaaagaggg caaaaaccct ctttttgata atttatataa agtaataatt ataatttata
     1261 catccatcca aattcatctg ttgcagtacc atatctaatt ccatttaatc tttcttttag
     1321 ttctgtagat accttccaac tatctacatc aaattccata ataaaatctt tatatttcaa
     1381 ttcttttatt aaagaaatag ttgctgcagt tcctgtacca aaaacttctt ttagttcgcc
     1441 agatttatat acatcaatca attcatctgt tgtaattttt ctttcaacca cttccaaacc
     1501 cattttcttt aaaatagtta tggcactatc tctggttaca cctgctaaaa tagttccatc
     1561 ttctaatgat ggtgttatgg ctttatttcc tataataaaa aacacattca ttgtacccac
     1621 ttcttgtaaa tacttatgtt caaatgcatc tgtccataaa acttggtcat agccagcttt
     1681 tcttgccatt gcagttggat acatacttgc agcataattt cctgcatttt tgctaaaacc
     1741 aacaccacca ggtgctgctc ttgtatattt ctcttctaca taaattttca ttggggcatt
     1801 ataataaggg cctgttgggc ttaatataat catgaattta tatgtttcag aaggtctaac
     1861 accaattaaa tgatctgtag aaaacataaa tggcctgata tataaagaat ggtctgtatg
     1921 cccaggaatc cagcttttat ctaattcaac taacatacgc atgccttcca taaaaatatc
     1981 ttcgggtaca gcaggcattt gcattctttc ggcagataca ttaaatctat tataattatc
     2041 ttttggccta aatataaaag catctccatt aggcgattta taagccttta tgccttcaaa
     2101 aatggcctgt ccgtagtgta acgatgccat tgaaggttca ataactaatg gctgatatgg
     2161 tttaatttct acattcttcc actcaccatt ttcataatca gcttcaagca tgtgatcagt
     2221 aaaatatttc ccaaaaggaa tattttctaa actcatttct tttaatttgc ttgattcagc
     2281 cttaataaca ttaatatcta ctgctgctgt cataactaaa tatatttgct gcaaagaaac
     2341 gtaatttttt aaaactaaca attgttaata ttgcacatgt aattatgaac gaacctattt
     2401 cactatccaa gtatgtaatt gccaagttat ataaagataa tatagtaatt acagataaaa
     2461 ttgaggaaaa tttagaacct gaaaagagtg cagatgttaa tattttgttt ttaggcgaaa
     2521 acaaaaaaaa tattgttatt ctgttaaaag aagaagatgc tatgcattta aaagacgata
     2581 gttttcagtt tttatcatcc attttacaag cttgtaagtt aaatatggcc gatgtagcaa
     2641 taattaatta caacaatcaa tcaataaatt tgaataaagt aactaaacaa tttgccccta
     2701 agtttgttat tatttttggc gaattgccca atacaataaa gcaggatgct actatagatt
     2761 tttatactcc aattaatatt aatagtgcag tttggttaaa agctgctgtt ttagatgaaa
     2821 tgaaggaaaa tagtaccaat gctaaagtag aaaaaagcaa attatggaat tgcttaaagc
     2881 aaatttttaa attataatga atgacagaaa aatttacttt aatttttgca accaataatg
     2941 caaacaaggt tcaagaaata aaatctgttt taggaaacaa ttttgagata attagtttaa
     3001 aagaagcagg aatagatatt gaaataccag agccttatga taccttaaaa gaaaatgcaa
     3061 cagaaaaatc gtctgttatt tttaggctaa caaataaaaa ttgttttagc gaagatactg
     3121 gattagaagt ggaagcttta catggagaac caggtgtaaa aagtgccaga tatgccaatg
     3181 aatatccaaa atttgaatcg aatattgaaa agcttttata caatttaaaa gacataaaaa
     3241 atagaaatgc tcaatttaga acagtaattt ctttaattat aaataaacaa gaatattttt
     3301 ttgaaggaat atgtaaaggt aaaattatag atactccaaa aggcaatgag ggctttggtt
     3361 acgaccccat ttttgtgcct gatggcagca ataaaacctt tgctgaaatg ccaattgaag
     3421 aaaaaaacaa atttagtcac agaaaaaaag ccatgcagca attaatactt tttctttccc
     3481 aaaataagac taatttttcc taattcaaga ttttttttgc attcatgcaa ctatttaatc
     3541 tctatttcta tcttgttttt atacagtgtt tttaagtacc taattattct atcaaaagca
     3601 tttattatgg catcatctgc tattgcattc aataatgatt tgttaaaaga aaactggaga
     3661 gtgaaacctt cctcatctac cataactaaa gaagctaagg tatcttacct aatggtggaa
     3721 caagttgaac caccaaaata tatgtgggat aaaatttgta aaatattaga tgcagaaaaa
     3781 gaagcagaaa acaaaaccaa ttatcaaaaa tcatctaata aaaccattaa agctttactg
     3841 attgcaggcg gtttaattac aattgcttcc atattacttt ttttacttta attgcaccag
     3901 ctacattcca attttttcaa ttttatactt aaaaaacttc taattcaata ttcttacact
     3961 attttgcaac aatgcaaaga gtaaaagttg aacttcctat taattttaat ttttctacta
     4021 ttattcctat tagaattact gatatgaatt atggtggaca tgtaggcaat gatagctttt
     4081 tatcgttaat acacgaagcc agaatgcagt atttgcagca ttttggttat acggaaatta
     4141 attttgaaaa tactagttta attatggccg atgcagctat agaatttaaa cacgaaatta
     4201 tttatacaaa tacaattaaa atttcggtac aagctgctgc cttcaccaaa attggatttg
     4261 atttatatta tcttttagaa attatcaatg gagaagaatt aataattgca ggcaaagcca
     4321 aaacaggtat gttatgcttt gactatacta ataaaaaaat aacaactgta cccacaaaag
     4381 ttattagtaa attaacaaca caataaacaa attacaatgc aacaaagaac ggagattgct
     4441 aacttaggag agtttggatt gatagaacat ctcactaaaa attttgaaat aaaaaatgca
     4501 tcaactatac taagtgttgg agatgatgct gctgtaattg accattttgg aaaacaaact
     4561 gttattacaa cagatatgtt ggtagagggt attcattttg atttaatgta caccccttta
     4621 aaacatttag gttataaaag tgtaatagta aacttaagcg atgtgtatgc aatgaatgct
     4681 ataccaacac aaattactat tagtattgct tttagcaaca ggtttagtgt agaagcctta
     4741 agcgaatttt atgaaggtgt ttatgcagct tgcgaaaaat atgctgtaga tttagttgga
     4801 ggtgatacaa cttcctctca aaaaggattt ataatatctg ttactgcaat tggcgaagtt
     4861 gcaccagata aatatgtaca aagaagtaca gcacaacaag gagacttaat ttgtgtatct
     4921 ggtaacacag gtggtgcttt tttaggttta acacttatgc aaagagaaaa aaaaatatat
     4981 cttgaaaacc ctgccataca accagactta gaaggagaag attatattgt aggcaggtta
     5041 ttaaagcctg aagccagaaa ggatattata gaattttttg ctgcaaataa cattgtacca
     5101 actgcaatga tggatgtaag tgatggcatt agtagcgagg ttttacacct ttgcaaacaa
     5161 agtaatgtag gttgtaaaat atttgaagat aaattaccca ttgatgaaaa aacaagagct
     5221 gctgctttta aatttggctt agaccccact gtttgtgcat taaatggcgg cgaagattat
     5281 gaattgattt ttaccctaaa acaagaagat tatgataaaa ttactttaaa tgaagaaata
     5341 gctgtaatag gctatatgac tgcattagaa gagggtaaca aattacaaac caaaggaggt
     5401 aatatttttg atattactgc acaaggttgg aacgcattta cataaagttt ataaaaaatt
     5461 gttctttcat tgaagaagaa attgatacat atttatttgg atagtatagt tatattattt
     5521 tccttgctta cttcatgcgt atcttataaa ataccaaatt atacctataa caaaaagcta
     5581 cctgtagcat tattaaaaga agatttaacc attttaaaaa aaactttaga agccaatcac
     5641 ccatctttat attggtacaa taccaaagac agtatagatt ttaattttga gaatgcactt
     5701 tctacaatga aggattcttt aaatgaagta cagtttaaaa aaaaggttgc tgaaattata
     5761 agtacaatac attgtggcca taccagtgtt aactattcta aaaattttac caagctttac
     5821 aaacatcacc tctaccccat gtttccttta tacttaaaag tgtgggcaga tagtatggtt
     5881 gttttaggta atacaattga aacagattcc atacttaaaa gaggcgttat tattaccact
     5941 attaatggag ttagcaatcg acttatttta gattcaattt ttaaaagtat tagtacagat
     6001 ggttatagtg ttaattacaa aaatcaggta gtaagcaata actttcctgc atggtataaa
     6061 caaacctggg gtttagatac aagttataca attagctatc tggatagttt aggaaataag
     6121 gcaactacta ccataaaaaa ttattcccct ataaaagata cttctacaat aaaaaaagaa
     6181 attacaaaaa ttaaaaagcc aaatagaaaa caacttaaaa atgcaaggcg tttagcaaaa
     6241 agatatttac aaattgatac t
//
LOCUS       DAOQHP010000090         6260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6802_length_6260_cov_3.728122, whole genome shotgun sequence.
ACCESSION   DAOQHP010000090 DAOQHP010000000
VERSION     DAOQHP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6802_length_6260_cov_3.728122"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLY81_04205"
     CDS             <1..472
                     /locus_tag="PLY81_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNF29460.1"
                     /translation="VPGRTGRNVSAATTIRLANEVPNIGGIKEASDDMNQCMQIIRDR
                     PDHFLVVSGDDALTMPQLSLGFDGVISVAAQAFPSQFTAMVNAGLAYNFNLAQKNHFK
                     LLEAYDLLFVENNPAGVKGFLYELGIIENVLRLPVVPLSNSVQEKVKTFLKNFK"
     gene            509..1192
                     /gene="cmk"
                     /locus_tag="PLY81_04210"
     CDS             509..1192
                     /gene="cmk"
                     /locus_tag="PLY81_04210"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNF29461.1"
                     /translation="MKKIIIAIDGFSSCGKSTLAKQLGAKLNYIFVDSGAMYRAIALY
                     FLQNNIDINKDIQVTEALKNIQLSFAFNNEKGSSDMFLNNINVEEQIREMAVSNNVSK
                     VSTLKPVRDFAVAQQQQMGIAKGIVMDGRDVGTVVFPTAELKIFVTASIDVRVERRYK
                     ELIEKNSNISKEEVKNNLQERDYIDSTRAIGPLKQAEDAKVLDNSHISREEQLAIALQ
                     WANAIIHSI"
     gene            1211..4615
                     /gene="mfd"
                     /locus_tag="PLY81_04215"
     CDS             1211..4615
                     /gene="mfd"
                     /locus_tag="PLY81_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNF29462.1"
                     /translation="MNLGVLHEQYKNNSCLLQLADRLLFADAQKIFLKNAQGSSPEFI
                     ITNIFSFTQTSELNHLIILNDSEEAAYFHNTLENLTNAYDLFYFPSSFKIRKNFKLLN
                     VSHVMLRTEMLTKLSVGGNKKIIVTYPEALFEKVVLPNTLHSNIISIKTNDTINLNSL
                     MELFVMYGFVRTDFVYEPGQFALRGGILDIYSFGNEKPYRIELFGNEVDSIRIFEPEK
                     QLSERKLLQVNIIPNVETQFESNDKISLLEFLSENTIIWLKDWDIIKEKLETQEEDFG
                     LFIERIKSNTNSTEEENEAEENTKILKEINLVDIVPATTIERQVQQKHIVEFGYKPYW
                     ANFEIEFNTQSQPSFNRNFDLLIQNLKKYETEKYSIYIFADQVKQLERLEAIFKDLQT
                     EIHFTPIPISIHEGFIDHQLKIVCYTDHQIFQRYHKYKVKQAFSKNKALTLKTLRDLQ
                     PDDFVTHIDHGVGRYSGLQKIEVNGKLQEAVRIIYKDSDILYVNINSLHKISKYTGKE
                     GTVPKVNKLGSDVWNKLKEKTKTKVKEIAFDLIKLYAQRKAQKGFSHTPDNYLQTELE
                     ASFIYEDTLDQAKAVADVKKDMESENPMDRLVCGDVGFGKTEVAIRAAFKTVVDGKQA
                     AVLVPTTILAFQHFKTFKERLRDFPLTVDFINRFKSSKEKKETLQKLQEGKIDIIIGT
                     HGLLGKDVKFKDLGLLVIDEEQKFGVAHKEKLKLLKTNVDSLTLTATPIPRTLQFSLM
                     GARDLSIINTPPPNRQPIHTEIQIFNDEFIRDTIYFETERGGQVFFIHNRVVGLPEMA
                     AFIQAQCPDLQVAYAHGQMEGSQLEEKILDFIDKKYDVLICTNIVESGVDIPNVNTII
                     INNAHQFGLSDLHQLRGRVGRSNKKAFCYLLAPPISTLPVDSRKRLQTLEQFSDLGSG
                     FQIAMRDLDIRGAGDMLGGEQSGFIAEIGFETYQKILDEAIRELKRTSFKELFKEEIS
                     KQDDYVNDCTIDTDLQILIPDFYIESITERLSIYTRMNDCKKEEELQLLYTEMIDRFG
                     NIPTEVDDLFTTVRCRWLAIDLGFEKMSLKENTMRCYFVNKNDSPYFESNTFKNIINF
                     LQKETNKGRLKQVAKNFLLVVDNINGMSEMLTFLNKMHNFVIAKNS"
     gene            4673..5827
                     /locus_tag="PLY81_04220"
     CDS             4673..5827
                     /locus_tag="PLY81_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNF29463.1"
                     /translation="MHIQSKLPQIGTTIFTTMSSLATEYNAVNLGQGFPDFEMNKELI
                     DLVHAAMLQGNNQYVHSNGLALLRDRIAEKYANLYQSNIDADKQITITPGGTYAIYTA
                     LTTVLNAGDEVIIFEPAYDSYIPNIVVNGAKPICISLQYPNYAIPWNEVRAKVNSKTK
                     MIIINSPHNPTGAVLSQEDINELRSIVANTNIFILSDEVYEHLIFDDLKNLSILRYQD
                     LLERSFVCFSFGKTYHCTGWKMGYCISSAALMQEFRKVHQFNCFSSNSPLQFALASYL
                     NNQDAYNLLGKQFQQKRDFFRNLMQQTPFKFIPSQGSYFECYSYEGLSNLSDKDFAIQ
                     LTKKLGVTVIPVSAFYHNEEDNKVIRFCFAKKEATLQLAANRLMQFENLF"
     gene            complement(5824..>6260)
                     /locus_tag="PLY81_04225"
     CDS             complement(5824..>6260)
                     /locus_tag="PLY81_04225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29464.1"
                     /translation="LMVGDGPERPAAEELVRNLNLETDVRFVGKQEQMEEILAVSDVF
                     VLPSEYESFGLAALEAMAAKAIVLSTNAGGLPEINIHCKTGFMADVGDVKTLANFAIT
                     ILQDEAKLNKMKMAAYQQACKFDLNSIIPLYEKLYSRFCRMN"
BASE COUNT         2346 a          903 c         1044 g         1967 t
ORIGIN      
        1 tgtaccaggt agaacaggac gaaatgtaag tgctgccacc actatacgtt tagctaatga
       61 agtacccaat attggaggta ttaaggaagc tagtgatgat atgaaccagt gtatgcaaat
      121 aattagagac aggcccgatc attttttagt agttagtgga gacgatgctt taacaatgcc
      181 acaactttca ttaggatttg atggtgttat aagtgttgct gcacaagcat ttccctcaca
      241 atttactgca atggtaaatg caggcttggc ttataatttt aacctagctc aaaaaaatca
      301 ttttaaactt ttagaagcat acgatttatt gtttgtagaa aataatcctg caggagtaaa
      361 ggggttttta tatgaactag gaattattga aaatgttttg cgtttacctg ttgtgccatt
      421 aagcaactct gttcaagaaa aagtaaaaac ttttttgaaa aattttaaat aaatttcctt
      481 ccaatttttt ctcaagatat tttagttaat gaagaaaata attattgcta ttgatggatt
      541 ttcatcttgc ggaaagagca ctttagcaaa acaattaggt gctaaactaa actacatttt
      601 tgtagatagt ggtgcaatgt atagagcaat agcattgtat tttttacaaa ataatattga
      661 tatcaataag gatattcaag taacggaagc tttaaaaaat atacaattat cttttgcatt
      721 caataatgaa aaaggcagca gtgatatgtt tttaaataat ataaatgtag aagagcaaat
      781 aagagaaatg gcagttagca acaatgttag caaagtaagt acattaaaac cagtacgaga
      841 ttttgccgta gcacaacaac aacaaatggg cattgcaaaa ggcattgtaa tggatggaag
      901 agatgttggc actgttgttt ttcctactgc cgaattaaaa atttttgtta cagcaagtat
      961 tgatgtacgt gtagaaagaa ggtataaaga attgatagaa aaaaattcta acataagtaa
     1021 agaagaagta aaaaataatt tacaagaaag agattatata gatagcacca gagctattgg
     1081 cccacttaaa caagcagagg atgcaaaagt gttagacaat agccatattt ccagagaaga
     1141 acaattagca attgcattac aatgggctaa tgcaataatc catagcattt aattatattt
     1201 gcacccctgt atgaatttgg gtgttttgca tgaacagtat aaaaataatt cctgcctttt
     1261 acaattggca gacaggttgc tatttgccga tgctcaaaaa atatttctaa aaaatgcaca
     1321 aggcagttcg ccagagttta ttatcactaa tattttttct ttcacccaaa catcagaact
     1381 taatcattta attatcttaa atgattctga agaggcagcc tattttcata ataccttaga
     1441 aaacttaacc aacgcttacg acctttttta ttttccatcc tcctttaaaa tacgaaaaaa
     1501 ttttaaacta cttaatgtat cgcatgtaat gcttagaaca gaaatgctta ctaagctttc
     1561 tgttggcggt aataaaaaaa taattgttac ttaccccgaa gccttatttg aaaaagttgt
     1621 actacccaat actttacata gcaatattat tagcataaaa accaatgata ctattaacct
     1681 aaatagttta atggagttgt ttgtaatgta tgggtttgta agaacagatt ttgtgtacga
     1741 acctggacaa tttgcattac gtggcggcat tttagatatt tactcatttg gcaatgaaaa
     1801 accctataga atagagctgt ttggcaacga agtagatagt ataagaattt ttgagccaga
     1861 aaagcaactt agtgaaagaa aactattaca agtaaatatt atacctaatg tagaaacaca
     1921 gtttgagagt aacgacaaaa tttctttact agaattttta tcagaaaata ccattatttg
     1981 gcttaaagat tgggatatta taaaagaaaa attagaaacc caagaagaag actttggatt
     2041 gtttatagaa agaataaaaa gcaatactaa tagcacagaa gaagagaatg aagcagaaga
     2101 gaatacaaaa attttaaaag aaattaattt agtagatatt gtacctgcta caacaattga
     2161 aaggcaagta caacaaaagc atattgttga atttggttat aaaccatatt gggcaaattt
     2221 tgaaatagaa tttaatacac aatcgcaacc ttcatttaat agaaattttg atttacttat
     2281 tcaaaattta aaaaaatacg aaacagaaaa atattccatt tacatatttg cagatcaagt
     2341 aaaacaatta gaaagattag aggccatttt taaagattta caaaccgaaa ttcattttac
     2401 accaattcca atttctatac atgagggttt tatagaccac caattaaaaa ttgtttgcta
     2461 taccgatcat caaatttttc aacgctatca caaatacaaa gtaaaacaag cgtttagtaa
     2521 aaacaaagct cttacattaa aaactttaag agacttacaa cccgacgatt ttgtaacaca
     2581 tatagatcat ggtgtaggca gatatagtgg tttgcaaaaa atagaagtta atggaaaact
     2641 acaagaagct gtacgaatta tttataaaga cagcgatatt ttatatgtaa acataaacag
     2701 ccttcataaa atttctaaat atacaggcaa agaaggcact gtgccaaaag ttaataaatt
     2761 aggtagcgat gtttggaata agctaaaaga aaaaactaaa accaaagtaa aagaaatagc
     2821 atttgattta ataaaactat atgcacaacg taaggcacaa aaaggatttt ctcatacgcc
     2881 agataattat ttacaaaccg agttagaagc aagctttatt tatgaagata ctttagacca
     2941 ggcaaaagca gtagccgatg tgaaaaaaga tatggaaagt gaaaatccaa tggatagatt
     3001 ggtatgtggc gatgttggct ttggtaaaac agaagtagcc attagagcag catttaaaac
     3061 tgtggttgat ggcaaacaag ctgctgtatt agttccaact acaattttag catttcaaca
     3121 ctttaaaact tttaaagaaa ggctcagaga ttttccatta acagtagatt ttataaatag
     3181 atttaaatca tctaaagaaa aaaaagaaac cttacaaaaa ctacaggaag gaaaaataga
     3241 tattatcata ggcactcatg gcttattagg caaagatgtt aagtttaaag atttagggtt
     3301 attagtaatt gatgaggaac aaaaatttgg tgtagcacat aaagaaaaat taaaattact
     3361 taaaaccaat gtagatagtt taacattaac agcaactcct ataccacgta cacttcaatt
     3421 tagtttaatg ggtgcaagag atttaagtat aataaataca ccaccaccaa acaggcagcc
     3481 catacatacc gaaatacaaa tatttaatga cgaatttatt agagacacta tttattttga
     3541 aacagaaaga ggagggcaag ttttctttat tcataacagg gtagttgggt tgccagaaat
     3601 ggcagctttt atacaagcac aatgccccga tttgcaagtt gcttatgcac atgggcaaat
     3661 ggaaggcagc cagttggaag aaaaaatttt agattttatt gataaaaaat atgatgtatt
     3721 aatttgtacc aatattgtag aaagtggtgt agatattcca aacgttaata ccataattat
     3781 aaataatgct catcagtttg gtttaagcga tttgcatcaa ttaagaggaa gagtaggaag
     3841 aagcaataaa aaagcttttt gttatttgct agccccacca attagtacat tgcctgtaga
     3901 tagtagaaaa agattacaaa ccttagagca gtttagcgat ttaggcagtg gctttcaaat
     3961 tgccatgaga gatttagata ttcgtggtgc aggagatatg cttggtggtg aacaaagtgg
     4021 ttttattgca gaaattggtt ttgaaaccta tcagaaaatt ttagatgaag caattagaga
     4081 acttaaaaga acctcgttta aagaattatt taaagaagaa attagtaaac aagatgatta
     4141 tgtaaacgat tgtacaatag atactgactt gcaaattctt attccagatt tttatataga
     4201 aagcattaca gaaagacttt ctatttatac cagaatgaat gactgtaaaa aagaagaaga
     4261 gttacaatta ttatacaccg aaatgataga tagatttgga aatataccaa cagaggtaga
     4321 tgatttgttt actactgttc gttgccgttg gcttgcaatt gatttaggtt tcgaaaaaat
     4381 gagcctcaaa gaaaatacca tgcgttgcta ctttgtaaat aaaaatgaca gcccttattt
     4441 tgaaagtaac acctttaaaa acataattaa ttttttacaa aaagaaacca ataaaggaag
     4501 gttaaagcaa gtagctaaaa attttttgtt agtagttgat aatatcaatg gtatgagtga
     4561 gatgctcacc ttcttaaata aaatgcacaa ctttgtaatt gctaaaaata gttaagcaaa
     4621 aaatctcccc taagctatat ttattcttgc tgcttctttt tatcttcgtt acatgcacat
     4681 acaatctaaa ttacctcaaa taggtacaac tatttttact acaatgagca gcttagctac
     4741 agaatacaat gctgttaatt tagggcaagg gtttccggat tttgagatga ataaagagtt
     4801 aattgatttg gtacatgctg ctatgttgca aggcaacaat caatatgtac atagtaatgg
     4861 gcttgcttta ttaagagata gaattgcaga aaagtatgcc aatctttatc aatccaatat
     4921 tgatgcagat aaacaaatta ctatcactcc gggtggcact tatgctattt acactgcact
     4981 aacaactgtt ttaaatgctg gagatgaagt aattattttt gaaccagcgt atgatagtta
     5041 tatacccaat attgtagtta atggggcaaa gcctatttgc atttctcttc aatatccaaa
     5101 ctacgccatt ccatggaacg aagtaagggc taaagtgaat agtaaaacta aaatgattat
     5161 tatcaattcg ccacataacc caactggtgc agttttaagc caagaggata ttaatgaatt
     5221 aagaagtatt gttgccaata ccaatatttt tattttgagc gatgaagtgt atgagcattt
     5281 aatttttgat gacctaaaaa atttaagtat tcttcgttat caagatttat tagaaagaag
     5341 ttttgtttgc ttttcttttg gcaaaaccta tcattgtact ggctggaaaa tgggttattg
     5401 tattagtagt gctgctctaa tgcaagagtt tagaaaagta catcagttta attgtttcag
     5461 cagcaacagc cccttacaat ttgcattagc aagttactta aataatcaag atgcatataa
     5521 tttactaggc aaacaatttc aacaaaaaag agattttttt agaaacttaa tgcagcaaac
     5581 accctttaag tttataccct ctcaaggtag ttattttgag tgttacagtt atgaaggatt
     5641 aagtaattta agcgataaag attttgctat acagctaaca aaaaagttag gggtaacagt
     5701 aatacccgtt tctgcatttt atcataacga agaagataat aaagtaattc gtttttgttt
     5761 tgcaaagaaa gaagctactt tacaacttgc agcaaacaga ttaatgcaat ttgaaaattt
     5821 attttagttc attctgcaaa acctactata caatttttca tataatggta taatactatt
     5881 caaatcaaat ttacaagctt gttgataagc tgccattttc attttattta atttggcttc
     5941 gtcttgtagt attgttattg caaaatttgc caaagttttt acatctccaa catctgccat
     6001 aaaacctgtt ttgcagtgta tatttatttc tggcaaacct cctgcattag tgcttaatac
     6061 aatagctttt gcagccattg cttctaatgc agctaagcca aaactttcat attcgctggg
     6121 tagtacaaaa acatcactta ctgcaagtat ttcttccatt tgttcttgtt tgcctacaaa
     6181 ccttacatcg gtttctaaat ttaagtttct aactaattct tctgctgcag gtctttctgg
     6241 tccatcgcca accattagta
//
LOCUS       DAOQHP010000091         6253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6816_length_6253_cov_3.515650, whole genome shotgun sequence.
ACCESSION   DAOQHP010000091 DAOQHP010000000
VERSION     DAOQHP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_6253_cov_3.515650"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLY81_04230"
     CDS             complement(<1..1123)
                     /locus_tag="PLY81_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF29465.1"
                     /translation="MAKEKSNTAKLDFKLEYAAAPESKSSAKINSKYGLFINGKFEKP
                     ESKKYFDTINPATEEKIATIAEANQTDVNKAVKAARTAYNNVWSKMPAKERAKYIYRI
                     ARMIQERARELAIIETMDGGKPIRESRDIDIPLAANHFFYYAGWADKLQYAFPNKKIA
                     PLGVAGQIIPWNFPLLMAAWKIAPALATGNTVVLKPAETTPLTALKLAEIIQESDLPP
                     GVVNIITGAGATGAAIVNHPDINKIAFTGSTDVGKIIQRAIAGTNKKATLELGGKAAN
                     IIFDDAPLDQAVEGVINGIFFNQGHVCCAGSRLFVQESVYNKVLQKLRDRIETLIVGD
                     PLDKNTDIGAINSKQQMDTILKHLKIGEKEGAVMHQSQCS"
     gene            complement(1187..2107)
                     /gene="deoC"
                     /locus_tag="PLY81_04235"
     CDS             complement(1187..2107)
                     /gene="deoC"
                     /locus_tag="PLY81_04235"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNF29466.1"
                     /translation="MAVTQTNFTIPDFSISPKIDQIGVEERAKRFTARSIKTTSKLSG
                     LKLVLNMIDLTTLEGKDTNGKVKQLCYKAQHLHNNYPDLPTVAAVCVYPSMVKVAKNA
                     LGNSGVKVAAVATAFPSGQAPKEVKITDTKFAVDNGADEIDMVISRGKFLQGEYNFVF
                     DEIATIKEACGNARLKVILETGELVTYDNVRKASDIAMYAGADFIKTSTGKIQPAATM
                     PVTLVMLEAIRDFYYKTGKMIAMKPAGGISKSKAALHYLVMLQETLGSAWMSNHWFRF
                     GASSLANDVLMQLEKERTGVYQSANYFSID"
     gene            2293..3237
                     /locus_tag="PLY81_04240"
     CDS             2293..3237
                     /locus_tag="PLY81_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF29467.1"
                     /translation="MEDKLKMFENRLLKVYKHKSKQAKRQEVCCYRVYDHDLPEFPFC
                     IELYEDKIYLAEYVRRHGMTDEEHEEWLEQCIVLICKLLNVPDENVFVKQRKRKSNRL
                     DQYEKTGEEKTFFTVLENGLKFLVNLSDYLDTGLFLDHRITRSMVKEQCAGKRVLNLF
                     AYTGSFSVYAAAGNAASVTTVDLSKTYLQWAEDNFTINHFKDKNKYNFIHADVKQYLK
                     TLQPNSFDLVIIDPPTFSNSKRMKDFLDTQRDHAELLNDILFALSENGVIYFSTNYTK
                     FVMDTENIKASYIKDITKATTPFDFEGKLKRWCFKIGK"
     gene            3297..4019
                     /locus_tag="PLY81_04245"
     CDS             3297..4019
                     /locus_tag="PLY81_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNF29468.1"
                     /translation="MLKYFFAFCIISICFSSCFIFGYRKIKGNGIYTTEKRNLSANKI
                     KVEGNLDIQIAQSNEPGVNIKADANLLEYIITELEDESLVIKTKRKYKLVSENPIIVE
                     VNTALLKSISIAGKSNVKGLTKFEGGEKLDIKIAGKGNVQLAVNTPKVEVKLAGVGEI
                     NLEGETKEVIVKISGSGNFKGKNLKAENATIKIAGSGDASVFASENLDVKISGSGNVE
                     YIGSPNIDQKISGSGTIKPIKE"
     gene            4147..5403
                     /locus_tag="PLY81_04250"
     CDS             4147..5403
                     /locus_tag="PLY81_04250"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNF29469.1"
                     /translation="MKVFKFGGASANSIERIQNIPEILQQYSTEQIVMVISAMGKTTN
                     ALEKVAEAFYNGNNIQALDLFEIVKQHHINTAKYLMITNYNNCINQLNDYFTEVEWLL
                     HDKPTQQYDYYYDQIVCIGEMMSTCVISCYFTEIGIGNTFLDVRDILKTDDNFRDAAV
                     NWEKTTANLKEIVLHPSLTIIQGFIGSTDENQSTTLGREGSDYTAAIFANLLHADSLT
                     IWKDVEGVMNADPKEFEDAQLINELSFNEVIEMAYYGAQVIHPKTIKPLQNKNIPLYV
                     KSFLDSSLKGTIISKQNNKNLPPIIVIKNNQVLMHLHTQDFSFVGEEPMAALYKIFST
                     LKIQPNLIQTGAISVQICLDNKEDKIEQLAINAASLFEVKVEKELQLLTIRHFTEELK
                     NKMLHNKNVILLQQTKQTLQALYTIE"
     gene            5461..5742
                     /locus_tag="PLY81_04255"
     CDS             5461..5742
                     /locus_tag="PLY81_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNF29470.1"
                     /translation="MAQYSLGDAMKKFIKQSNLRNGLRAVQIEEVWEEVMGKTIAKYT
                     DKIEIVNQTLFIKTQIGSLKNELMYQKPLIIERINEKFGEKIIATVVIQ"
     gene            complement(5773..>6253)
                     /locus_tag="PLY81_04260"
     CDS             complement(5773..>6253)
                     /locus_tag="PLY81_04260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF29471.1"
                     /translation="ELDAEKKREEKTIKDYATKHNLKAVYTQNGVGVVVETMGDTAFK
                     ADSGKQVSVYYSGKLMDSGKEFDSNVKNGVKANPFIFTLDAKMVIPGWDEAIKYFAKG
                     GKGKLLIPALMGYGAQGSAPVIPPYANLVFDVEITDVTDAPKLQPTTPPITVQPVTK"
BASE COUNT         2147 a          896 c         1028 g         2182 t
ORIGIN      
        1 tactgcattg gctttggtgc attactgctc cttctttttc gccaattttt aaatgcttta
       61 aaatagtatc catttgttgt ttagagttaa tggcacctat atcagtattt ttatctaatg
      121 ggtctcctac aattaaagtt tcaattctat ctcttaattt ttgtaatact ttgttgtaaa
      181 cagattcttg tacaaatagc ctggaaccag cacaacaaac atgcccctgg ttaaaaaata
      241 taccatttat tacgccttca acagcttggt ctaaaggggc atcatcaaaa attatgttgg
      301 cagctttacc tcctaattct aaagttgctt ttttatttgt gccagcaata gcacgttgaa
      361 taattttacc aacatcggta gaaccggtaa atgcaatttt attaatatct gggtgattta
      421 caattgcagc acctgtagct cctgcacctg ttataatatt tacaacacct ggtggcaaat
      481 cactttcttg aataatttcg gctaatttta atgctgttaa tggagtagtt tctgctggtt
      541 ttaaaacaac tgtatttcct gtggctaaag caggggcaat tttccatgca gccattagta
      601 atggaaaatt ccaaggtata atttggccag ctacacctaa tggagctatt tttttattag
      661 ggaatgcata ctgcaattta tctgcccaac ctgcataata aaaaaaatgg ttagctgcaa
      721 gtggtatatc aatatctcta ctttctctta tgggcttacc tccatccatt gtttcaatta
      781 tcgctaattc tctagcacgt tcttgaatca ttcttgcaat acgatatata tattttgctc
      841 tttcttttgc aggcattttg ctccatacat tattgtatgc tgtacgagct gcttttactg
      901 ctttatttac atcagtttga ttggcttcgg ctatggttgc aattttttct tcagtagcag
      961 ggtttatggt gtcaaaatat tttttgcttt ctggtttttc aaatttacca ttaataaaca
     1021 aaccgtattt tgaattaatt tttgcactgc tcttactttc tggtgctgct gcatattcta
     1081 atttaaaatc tagttttgca gtattgcttt tttcttttgc cattctttta tatttaaaag
     1141 gttctaatgg ttattggtgt aattattttt aatagtaata atagtatcaa tctatactaa
     1201 agtagttggc actttggtaa actcctgttc tttctttttc taattgcatt agtacatcgt
     1261 tggctaagct gcttgctcca aatctaaacc aatgattact catccatgca ctgcctaatg
     1321 tttcttgtaa cattactaaa taatgcaatg cagctttgga tttacttatg ccaccagcgg
     1381 gtttcatggc aatcattttt cctgttttat aataaaaatc tctgatagct tctaacatta
     1441 caagtgtaac aggcattgta gctgcaggtt gtatttttcc ggtagaggtt ttaataaaat
     1501 ctgcacctgc atacatggct atatcgcttg cttttctcac attgtcgtag gttactaatt
     1561 caccagtttc taaaattact tttagtcttg catttccaca ggcttcttta atggttgcaa
     1621 tttcatcaaa aacaaaattg tattcacctt gtaaaaattt accacggcta attaccatgt
     1681 caatctcatc ggcaccatta tctacagcaa atttggtgtc tgtaatttta acttcctttg
     1741 gtgcctgtcc acttgggaag gcagtagcca cagcagctac ttttactcca gagtttccta
     1801 aagcattttt ggcaactttt accatacttg gatatacaca tactgcagca acggttggta
     1861 aatctggata attattatgt aaatgttgag ccttataaca aagttgtttt actttaccat
     1921 ttgtgtcctt cccttctaaa gttgttaagt caatcatatt taaaacaagc tttaaaccac
     1981 ttaatttaga agttgttttt atacttcttg cagtaaagcg ttttgctctt tcttctactc
     2041 ctatttggtc tattttagga gaaattgaaa agtcggggat tgtaaaattt gtttgtgtta
     2101 cagccataat cattttttgt aaatatcaaa tgtaaatgat ttagtattaa attagaaatt
     2161 ttagcaaaga gtttaaataa gattatataa ccgatttttg tattgttgcc aagtgtacaa
     2221 actgtacaag tgtgcgacgc aacaaatgct gcaaaatgtt gaaatgttga ttttaaaatt
     2281 ttaattattg taatggaaga taaattaaaa atgtttgaga accgtttact aaaggtatat
     2341 aagcataaaa gcaagcaggc taaaaggcaa gaagtatgtt gttatagagt gtacgaccat
     2401 gatttgcctg agtttccttt ttgcattgaa ttgtacgaag ataaaattta tttagctgaa
     2461 tatgtaagaa ggcatggtat gactgatgaa gagcatgaag aatggttaga acaatgtatt
     2521 gtactaattt gtaaattgct aaatgtgcct gatgaaaatg tttttgtaaa acaacgaaaa
     2581 agaaaaagta accgattaga tcaatatgaa aaaacgggag aagaaaaaac atttttcact
     2641 gttttagaaa atggattaaa atttttagta aatctttctg attatttaga tactggtttg
     2701 tttttagatc atagaattac cagaagcatg gttaaggaac aatgtgcagg taaaagggta
     2761 ttaaatttat ttgcttacac tggctccttt agtgtgtatg ctgcagcagg taatgctgca
     2821 agtgtaacta ctgttgattt aagtaaaact tatttgcaat gggccgaaga taatttcacc
     2881 attaatcatt ttaaagataa aaacaaatat aactttatac atgccgatgt aaagcaatat
     2941 ttaaaaacct tgcagcccaa tagttttgat ttggtaatta ttgacccacc cacatttagt
     3001 aacagtaaaa gaatgaaaga ttttttagac acacaaagag accatgctga attattaaat
     3061 gatatactct ttgcattatc agaaaatggt gtaatttatt ttagcactaa ttataccaaa
     3121 tttgtgatgg atactgaaaa tattaaagca tcttatatta aagacataac taaagctact
     3181 actccatttg attttgaagg aaagctaaaa agatggtgtt ttaagatagg taaataaccg
     3241 ttataaatgg taattgaaat tcataatgtt tatttatttt tataaaaaaa attgatatgc
     3301 taaaatattt ttttgctttt tgtattattt ccatttgttt ttcctcttgt tttatttttg
     3361 ggtatagaaa aataaaaggg aacggtattt atacaactga aaaacgcaat ttatctgcca
     3421 ataaaattaa agtggaaggt aatttagata tacaaattgc acaatctaac gaaccaggtg
     3481 ttaatataaa agctgatgcc aatttattag aatatataat aactgaatta gaagatgaaa
     3541 gccttgtaat taaaacaaaa agaaagtata agcttgtatc ggaaaaccca attattgttg
     3601 aagtgaatac ggcattatta aaatctatta gtattgctgg taaaagcaat gtaaaaggct
     3661 taactaaatt tgagggtggc gaaaaattag atattaaaat tgctggtaag ggtaatgtgc
     3721 aacttgcagt taacactcct aaagtagaag ttaaacttgc tggtgttggt gagataaatt
     3781 tagaaggtga aacgaaagaa gtaattgtga aaatatcggg tagtggtaat tttaaaggca
     3841 aaaatttaaa agcagagaat gctactatta aaattgcagg cagtggagat gcatctgtat
     3901 ttgcctcaga gaatttagat gttaaaatat cgggtagtgg taatgtggaa tatataggaa
     3961 gccctaatat tgatcagaaa atttcaggta gtggtactat taaacctata aaagagtaag
     4021 aaagttatct aataaataaa aagacacaac aatactcttt aaaaattttg ctataaatat
     4081 ttagttaact tttaaattaa gcttacactc aacaagtatc tttgaggcat ttaattattt
     4141 aatcgtatga aggtttttaa atttggagga gctagtgcaa attctataga aaggatacaa
     4201 aacattccag aaatattaca gcaatatagt acagagcaaa ttgtaatggt tatttctgca
     4261 atgggtaaaa ctactaatgc acttgaaaaa gttgctgagg ccttttataa tggtaacaat
     4321 atacaagctt tagatttatt tgaaattgta aaacaacacc acattaatac tgctaagtat
     4381 ttaatgatta ctaattataa caattgcata aatcaattaa acgattattt tactgaagtg
     4441 gaatggttac tacatgataa acctacgcag caatacgatt actattatga tcaaattgtt
     4501 tgtattggtg aaatgatgag tacttgtgtg attagttgct attttactga aataggcatt
     4561 ggtaatacct ttttagatgt tagagatatt ttaaaaacag atgataattt tagagatgct
     4621 gcagtaaatt gggagaaaac aactgccaac ttaaaagaga ttgtattgca tccaagcctt
     4681 acaattattc aagggtttat tggaagtaca gacgaaaacc agagtaccac acttggtaga
     4741 gaaggtagcg attatactgc agctattttt gccaatttat tacatgcaga tagtttaact
     4801 atttggaaag atgtagaagg tgtgatgaat gctgacccta aggaatttga agatgcacag
     4861 ttaattaatg aactaagttt taatgaagta attgaaatgg catattatgg agcccaggtt
     4921 attcatccta aaacaataaa accactacaa aataaaaata ttccattgta tgttaaatct
     4981 tttttagata gtagtttaaa aggaacaatt atttctaaac aaaataataa aaacttacct
     5041 cctattattg tgataaaaaa taatcaggtt ttaatgcact tgcatacaca ggattttagt
     5101 tttgttggcg aagagccaat ggctgcttta tataaaatat tttctacatt aaaaattcag
     5161 ccaaacttaa ttcaaacagg tgcaataagt gttcaaatat gtttagataa taaagaagat
     5221 aaaattgaac aacttgcaat taacgcagca agtttatttg aagtgaaagt agaaaaagag
     5281 cttcagttat taacaataag gcattttaca gaagaactta aaaacaaaat gctacacaat
     5341 aaaaatgtta ttttactaca acaaactaaa caaaccttgc aagctttata cactatagaa
     5401 taataaactt gcaaaaagtt attgaattgt ttatgtattt ttatgttata attttaacct
     5461 atggcccaat atagtttagg cgatgcaatg aaaaaattta ttaagcaaag taatttgcgt
     5521 aatgggctta gagcagtgca aattgaagaa gtatgggaag aagtaatggg caaaacaatt
     5581 gcaaaataca ccgataagat tgaaattgta aatcaaacac tttttattaa aacgcaaata
     5641 ggctctttaa agaacgaatt aatgtaccaa aaaccattaa taattgaaag aataaatgag
     5701 aagtttggag aaaaaataat tgctactgta gttatacaat agcaattact ttaataggca
     5761 ataattatat ttttattttg ttacaggttg tactgttata ggtggtgttg taggttgcaa
     5821 ttttggggca tcagtaacat ctgtaatttc tacatcaaaa acaaggttgg cataaggagg
     5881 aataactgga gcagagcctt gtgcaccata gcccataaga gctggtatta ataattttcc
     5941 tttaccacct ttagcaaaat atttaatggc ttcatcccat cctggaataa ccattttagc
     6001 atctagggta aaaataaaag gattagcttt tacaccattt tttacattac tatcaaactc
     6061 tttaccagaa tccattaatt tgcctgaata atatactgat acttgtttac cagaatctgc
     6121 tttaaatgct gtgtcaccca tagtttcaac aactacacct actccatttt gggtataaac
     6181 tgcttttaag ttatgtttag ttgcatagtc ttttattgtt ttttcttccc gttttttttc
     6241 tgcatctaat tct
//
LOCUS       DAOQHP010000092         6221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6869_length_6221_cov_3.389880, whole genome shotgun sequence.
ACCESSION   DAOQHP010000092 DAOQHP010000000
VERSION     DAOQHP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6869_length_6221_cov_3.389880"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLY81_04265"
     CDS             complement(<1..309)
                     /locus_tag="PLY81_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF29472.1"
                     /translation="MTKEKILVIGASGQIGVELTLALRNLYGNNNVIASDLREQNPLL
                     EGTGPYVSLDVMNKEMLHVQVIRQNITQIYLLAAILSATGEKNPNLAWHLNMQGLLNV
                     L"
     gene            441..1265
                     /gene="kdsA"
                     /locus_tag="PLY81_04270"
     CDS             441..1265
                     /gene="kdsA"
                     /locus_tag="PLY81_04270"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNF29473.1"
                     /translation="MNYSYLNNLFSNQTYHKQNFFLIAGPCVVESEEIVTEVAETVAN
                     VCKKLQIPYIFKASYRKANRTSATSFTGLGDELALDLIKKVGTTYNLPTLTDVHAHEE
                     CAMAANFVDVLQIPAFLCRQTDLLVAAAQTNKIVNVKKGQFLSGQSMQFAVDKIVKAG
                     NNKVMLTERGTTFGYQDLVVDYRNITWMKEHGTPVIMDCTHSLQQPNQTTGITGGNPA
                     LIGTIAKAAIATGADGLFIETHPNPSKAKSDGANMLPLHLLENLLEQLVKIRSVIQ"
     gene            1489..2679
                     /locus_tag="PLY81_04275"
     CDS             1489..2679
                     /locus_tag="PLY81_04275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF29474.1"
                     /translation="MKLSTLLNKFNEPETLKMAKLGRELRAQGKDVIDLSLGEPDFDT
                     PTHIKEAAIKAINDNWSHYTPVPGFLDLREAVCTKLKRDNNLDYLPENIVVSTGAKQS
                     LANAILALVDVNDEVIIPTPFWVTYSELVKIAGGKVVTISTTPEAKFKITPQQLEASI
                     TKNSKVFMFSSPCNPSGAVYSKQELAELVKVFEKYPNVFIISDEIYEFINYKDGHNSI
                     AQFSSIKDRVVVINGLSKGYAMTGWRLGYLAANTTIAKACEKLQGQFTSGTCSITQKA
                     AVVALTADLKPTFEMTEEFAKRKKRVLEIVKQIPGFKCTEPEGAFYIFPDVSYYYGKS
                     DGVTTISNASDLSMYILNTAYVSSVMGDAFGEPNCVRFSFANSLENIEKAWERIIAAL
                     NKLH"
     gene            complement(2782..3447)
                     /gene="rsmI"
                     /locus_tag="PLY81_04280"
     CDS             complement(2782..3447)
                     /gene="rsmI"
                     /locus_tag="PLY81_04280"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNF29475.1"
                     /translation="MLYLVPTPIGNLKDFTYRAVEILKGVDVILCEDTRTSSRLLDYY
                     EINKPLSPYHKFNEHKIVAHLVQQMQAGKVIALITDAGTPGISDPAFLLVRECIKENI
                     VVECLPGATAFVPALVNSGLPTNNFVFEGFLPLKKGRQTKLKQLATEDRTIVIYESPM
                     RLVKTLEELAMYFGNDRLCCVSRELTKMFEENKRGTLKENANYFAQKTVKGEIVITIA
                     GAE"
     gene            complement(3460..3708)
                     /gene="purS"
                     /locus_tag="PLY81_04285"
     CDS             complement(3460..3708)
                     /gene="purS"
                     /locus_tag="PLY81_04285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNF29476.1"
                     /translation="MLYNIQIKVMPLKDLLDPQGKAIQNGMKNLGITSVTDVRVGKHV
                     TLQIDAANENEAKLIAEEAGKKLLANPVMEFFEIELVN"
     gene            complement(3734..4495)
                     /gene="pssA"
                     /locus_tag="PLY81_04290"
     CDS             complement(3734..4495)
                     /gene="pssA"
                     /locus_tag="PLY81_04290"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNF29477.1"
                     /translation="MIKQIPNLFTLLNLFFGCLAVVTSLQSGLTIVLDGYGEQLVEIP
                     ESIFMASIFIGIAAIIDFLDGFVARLFKATSEIGKQLDSLADVVSFGVAPSIIVYQFL
                     RLSFAQQEGGLDVNTLWLMPAFIIPCCGAYRLARYNLDTQETSSFKGVPIPAVGLLIA
                     SYPIVYWYSGNANYLINLLRNQWFWYAIIILVSYLMVSTLPMLSLKFKGVSFKNLLPI
                     ILILLISFIAAFFVGWFAVTIGFITYVIVSLLFKN"
     gene            complement(4519..5814)
                     /locus_tag="PLY81_04295"
     CDS             complement(4519..5814)
                     /locus_tag="PLY81_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNF29478.1"
                     /translation="MKYLPLNPEIFIHNRKRFVKEMAPNSIAIIVSNDEFPSNGDALH
                     SFKQNSDLYWLTGIEQEDSLIILFPDNPDPKYREVLVLVRPNELKEKWDGKRLRANEG
                     TSISGIQTIVWLDSLDALMQAWIHLADNIYLDSNENDRKANLIRSREYRFIDEMKARY
                     PLHHYKRAAQIFGKLRAIKSKEEVEVIQHAINITDNTFRRLLQFIQPGVFEYEIEAEL
                     IHSFLSQRATGEAYGSILASGDRARTLHYVSNNQECRDGELILMDFGAAYGGYCADLT
                     RTVPVNGKFTKRQKIVYNACLHLHNYAKSILKPGISIVDYTDKVGEEATQQFLKIGLL
                     KKMDIKNEDKDNRAYRKYLYHGISHHLGIDVHDLGTRTAPIQAGMVFTVEPGIYIEEE
                     QMGIRIENNLWITKNGHIDLMKNIPITVEEIEALMKKQK"
     gene            complement(5897..>6221)
                     /locus_tag="PLY81_04300"
     CDS             complement(5897..>6221)
                     /locus_tag="PLY81_04300"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNF29479.1"
                     /translation="NFSEKSAYQPKSLGNQMIENIIINTVVPMLFAYGLYNKEEHYKT
                     KAIEWLQALSPEKNSVITEWKNTEITAQTAFDTQALLQLNKFYCTKLNCLSCAVGVKI
                     LRASN"
BASE COUNT         2140 a          984 c          945 g         2152 t
ORIGIN      
        1 taaaacattt aataaacctt gcatatttaa gtgccaagca aggttaggat ttttttcgcc
       61 tgttgcactt aaaattgctg ccaataaata aatttgagtg atgttttgcc ttattacttg
      121 tacatgtaac atttctttgt tcattacatc taaacttaca taaggtcctg taccctccaa
      181 taaaggattt tgttctctta agtcagatgc aataacattg ttattaccat atagatttct
      241 taaagcaagg gttaattcaa ccccaatttg tccacttgca cctattacta aaattttttc
      301 tttggtcata ataaggatta taaaattcta aaatgctaaa atgcaaagaa aaggaactta
      361 ggaatataaa gttttaacca tttaaaagcc ttttattatt ttctatttaa cctatgtttg
      421 caacttgtaa tttttattgt atgaattata gttatttgaa caatttattt agtaatcaaa
      481 cttaccataa gcaaaacttt tttcttattg ccgggccatg tgttgtagaa agtgaagaga
      541 tagtaacaga ggttgccgaa actgtggcaa atgtttgcaa aaaactacaa ataccttata
      601 tttttaaagc aagctacaga aaagctaata gaacaagtgc aacatctttt acaggattag
      661 gagatgagtt ggctttagat ttaataaaga aagttggtac aacttataat ttgcccacat
      721 taactgatgt acatgcacat gaagaatgtg caatggctgc caattttgtg gatgttttgc
      781 agatacctgc ctttttatgt aggcaaacag atttgttagt tgcagcagcc caaacaaata
      841 aaattgtaaa tgtaaaaaaa ggacagtttt taagtgggca aagtatgcaa tttgcagtgg
      901 ataaaattgt aaaagcaggc aacaacaagg taatgcttac tgaacgtgga actacatttg
      961 ggtatcagga tttagttgtt gactatagaa atattacctg gatgaaagaa catggtacac
     1021 cagtaattat ggattgtaca catagccttc aacaacctaa ccaaacaaca ggaattactg
     1081 gcggaaatcc tgcattaatt ggcacaatag ccaaagctgc catagcaact ggtgctgatg
     1141 gcttgtttat agaaacacac cctaatccat ctaaagcaaa aagcgatggt gccaatatgc
     1201 tgccattaca tttattagaa aatttattag aacagttagt gaaaattaga tcagtaatac
     1261 aataaaaaaa aatgctcttg aaaacaagag catctcataa gttatcaacg gtattgcacc
     1321 ctttacataa aattaactta tgcaaaagtg aacttttgtt ttttatttta ataacctcta
     1381 aaaacataat attaacccaa ttagacattt taatttagca caacatttat atttacaatt
     1441 caaataaaaa ttaataataa tttcaatagt atttaaaatt tttttgttat gaagttatct
     1501 accttattaa acaaattcaa tgaacctgaa actttaaaaa tggcaaaact tggccgtgaa
     1561 ttaagagcac aaggaaaaga tgtaattgac ttaagtttag gagaacccga ttttgataca
     1621 ccaacgcata ttaaagaagc tgctattaaa gcaattaatg ataattggag ccactataca
     1681 ccagttccag gatttttaga tttgagagag gcagtatgta ctaaattaaa aagagataat
     1741 aacttagatt acttacctga gaatattgta gtttcaacag gtgcaaaaca aagcttagca
     1801 aatgcaattt tagcattggt tgatgtgaat gatgaagtaa ttataccaac gcctttttgg
     1861 gttacttaca gcgaattagt aaaaattgca ggaggtaaag tagttacaat tagtacaacc
     1921 cccgaagcaa aatttaaaat tacacctcaa cagttagaag cttctattac aaaaaattct
     1981 aaggttttta tgttttcatc gccttgtaac ccaagcggtg ctgtatatag caaacaagag
     2041 ttggctgaat tagtgaaagt gtttgaaaaa tatccaaatg tttttattat ttcagacgag
     2101 atttatgaat ttattaatta caaagatgga cataatagta ttgcacaatt tagttccatt
     2161 aaagataggg tagtggtgat aaatggatta agtaaaggct atgcgatgac tggttggcgt
     2221 ttaggatatt tagctgcaaa tacaactatt gcaaaagctt gcgaaaaatt acaaggacaa
     2281 tttacaagtg gcacttgttc tattacacaa aaggcagcag tagtagcact tacagcagat
     2341 ttaaaaccta cttttgaaat gactgaagag tttgcaaaaa ggaagaagag agtgttagaa
     2401 attgtaaaac aaataccagg ctttaaatgc accgaaccag aaggagcttt ttatattttt
     2461 cctgatgtga gttattatta tggtaaatca gacggagtta caaccatttc taatgcttct
     2521 gatttaagta tgtatatttt aaatactgct tatgtaagta gtgtaatggg agatgctttt
     2581 ggggaaccta attgtgttcg tttttctttt gcaaatagct tggaaaatat agaaaaagca
     2641 tgggaaagaa ttattgctgc attaaataaa ttgcattaat tattttgaaa tgaaaaagaa
     2701 taaaagccct tcttatttta atgtataaga agggcttttt aaataacaat caatttgtaa
     2761 tgaagtaatt ttgaagtgca actactctgc accagcaatt gtaattacaa tttcaccttt
     2821 tactgttttt tgagcaaaat aattggcatt ctctttcagc gttccacgtt tgttttcttc
     2881 aaacattttt gttaattctc tacttacaca acaaagccta tcattaccaa agtacattgc
     2941 aagctcttct aaggttttta ctaaacgcat tgggctttcg taaattacta ttgttctatc
     3001 ttcggttgct aattgtttaa gtttggtttg ccttcctttt tttaagggta aaaaaccttc
     3061 aaaaacaaag ttattggttg gcaatccact attaaccaag gcaggcacaa aagcagttgc
     3121 accaggcaaa cattctacca caatattttc tttaatacat tctctaacta ataaaaatgc
     3181 tgggtcgcta atacctggtg tgccagcatc tgtaattaag gcaattactt taccagcttg
     3241 catttgctgt actaaatgag caacaatttt atgttcgtta aatttatgat agggagaaag
     3301 tggtttatta atttcgtaat aatctaaaag ccgagatgag gttcttgtat cttcacataa
     3361 tattacatca acacctttta aaatttcaac agcacggtaa gtaaaatctt ttaagttacc
     3421 tattggtgtt ggtacaaggt aaagcatttt atattagcat taatttacaa gttcaatttc
     3481 aaaaaattcc attactgggt ttgccaatag tttttttcct gcttcttcag caattaactt
     3541 tgcttcgttt tcattggcag catcaatttg taatgttaca tgtttaccta ctcttacatc
     3601 tgtaacactg gtaataccta aattcttcat cccattttga atagcttttc cttgtgggtc
     3661 taataaatct tttaaaggca ttacttttat ttgaatatta tacaacattt gatttttttt
     3721 ttgtatgtaa atattaattt ttaaaaagta aagatacaat tacataagta ataaaaccta
     3781 tggtaactgc aaaccagcca acaaaaaatg ctgcaataaa acttattaat aaaataagta
     3841 taattggaag aagattttta aagcttactc ctttaaattt taaagagagc atgggtaatg
     3901 tacttaccat tagatagctc actaaaatta tgatagcata ccaaaaccac tgatttctta
     3961 ataagtttat taaataattt gcattgccac tataccaata tacaattgga taagatgcaa
     4021 taagtaaacc aactgctggt attggaacac ctttaaaact tgaagtttct tgggtatcta
     4081 aattatatct ggctaatcta tatgcaccac aacatggtat gataaaagca ggcataagcc
     4141 ataaagtatt aacatctaaa ccaccttctt gttgtgcaaa tgataaacgt aaaaactgat
     4201 atacaataat ggaaggtgca acaccaaaac ttactacatc tgctaaacta tctaattgct
     4261 ttcctatttc ggaagttgct ttaaacaacc ttgcaacaaa accatctaaa aaatcaatta
     4321 ttgctgcaat gccaataaaa atacttgcca taaaaatgct ttcaggtatt tctactaatt
     4381 gttcaccata tccatctaac acaatagtta aaccactttg taaagaggtt acaacggcta
     4441 aacaaccaaa aaataaattt aatagagtaa ataaattggg aatttgtttt atcattgaat
     4501 aagaatattt tgctaatatt atttttgttt tttcattaaa gcctcaattt cttcaacagt
     4561 aatcggaata tttttcatta aatcaatatg tccgtttttt gttatccata agttgttttc
     4621 aattctaatt cccatttgtt cttcttcaat ataaatgcct ggctctacag taaaaaccat
     4681 gcctgcttgt attggagcag ttcgtgtacc taaatcatgc acatcaattc ctaaatgatg
     4741 gcttatacca tggtataaat atttacgata ggctctattg tctttgtctt cattttttat
     4801 atccatcttt ttaagtagtc ctatttttaa aaattgctgt gttgcttctt cgccaacttt
     4861 atcggtataa tcaacaatag atattccagg ttttaaaatg cttttagcat aattgtgtaa
     4921 atgtaaacaa gcattataaa ctattttttg gcgtttggta aattttccgt ttaccggtac
     4981 agttctggtt aaatctgcac aatagccacc ataagcagca ccaaagtcca tcaatattaa
     5041 ttctccgtct ctacattctt ggttattgct tacataatgt aaggttcttg ccctgtctcc
     5101 actggctaaa atgctaccat aggcttctcc tgtagctcgt tggcttaaaa agctatgtat
     5161 taattctgct tcaatttcat attcaaaaac acctggttgt ataaattgta aaagtcttct
     5221 aaaagtatta tctgtaatat ttatggcatg ttgtattact tcaacttctt ctttagattt
     5281 aattgctcta agttttccaa aaatttgggc tgctctttta taatggtgta aagggtaacg
     5341 agccttcatt tcatcaataa atctatattc tctgctacgt ataaggttag cttttctatc
     5401 attttcattg ctatctaaat aaatattatc tgctaaatgt atccaagctt gcattaaagc
     5461 atccaggcta tctaaccata ctatagtttg tatgccagaa atggaagtac cttcgttggc
     5521 tcttaatctt ttaccatccc atttttcttt taattcatta ggacgaacta aaaccaatac
     5581 ttctctatat tttggatctg gattatctgg aaaaagtata atcaaggaat cttcttgttc
     5641 aatgccagta agccaatata aatcgctgtt ttgtttaaaa gaatgaagag catcgccatt
     5701 gctaggaaat tcatcgttac ttacaataat ggctatactg ttgggtgcca tttcttttac
     5761 aaaacgctta cgattatgaa taaaaatctc tgggttcaat ggtagatatt tcatatcaaa
     5821 aattttaaag tttgcaagat ataacaattt atacagagca aataaaataa aactgctaaa
     5881 gggtaacaga ttttttttaa ttgctagctc ttaaaatttt tacaccaaca gcacaactca
     5941 aacaatttaa tttggtgcag taaaatttat ttaattgtaa tagagcttgt gtatcaaatg
     6001 cagtttgggc ggtaatttca gtatttttcc actcggtaat tacagaattt ttctcaggcg
     6061 ataacgcttg taaccattct atagctttgg ttttgtaatg ttcttcctta ttatacaaac
     6121 cataagcgaa aagcattggt acaacagtat taataataat attttctatc atttggttgc
     6181 ctaagctttt gggttgataa gctgattttt cactaaaatt g
//
LOCUS       DAOQHP010000093         6198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6899_length_6198_cov_3.347062, whole genome shotgun sequence.
ACCESSION   DAOQHP010000093 DAOQHP010000000
VERSION     DAOQHP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_6198_cov_3.347062"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLY81_04305"
     CDS             complement(<1..266)
                     /locus_tag="PLY81_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29480.1"
                     /translation="MKIHLAFTISLFISIQIVAQNTPVLKKQISLTEINNVLNNSNQN
                     TFLVSGNDNYTLIEQASKIQLGTLQVDVGSYSLPGHKQTYLWNN"
     gene            complement(324..779)
                     /locus_tag="PLY81_04310"
     CDS             complement(324..779)
                     /locus_tag="PLY81_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29481.1"
                     /translation="MEIFNADFADFIKSLNKHDVEYMLVGGYAVILRGYSRSTGDMDI
                     WVNKTEPNFTQLQKAITEFGLPLAAIPKEKFFSEDYDVFSFGRPPYAIEIMTALKGVA
                     FDEAFAHATIQKVENVEIRVIHLNQLLQAKKAAGRHKDLNDIENLPQSN"
     gene            complement(780..989)
                     /locus_tag="PLY81_04315"
     CDS             complement(780..989)
                     /locus_tag="PLY81_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29482.1"
                     /translation="MKTSFLDRTVFEARNMTKDGNIDNNYWHDKSIEEKMQAAAIVTA
                     AAFGEPDFLKKKVERTIFTSRKHKK"
     gene            complement(1197..3233)
                     /gene="uvrB"
                     /locus_tag="PLY81_04320"
     CDS             complement(1197..3233)
                     /gene="uvrB"
                     /locus_tag="PLY81_04320"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNF29483.1"
                     /translation="MTFKLHTSYQPSGDQPTAIQQLVDGVNNGEQFQTLLGVTGSGKT
                     FTIANVIEKIQRPTLVLTHNKTLVAQLYGEFKQFFPDNAVGYFVSYYDYYQPEAYMPV
                     SNVFIEKDLSINEELDKLRLQATSQLLSGRRDVIIVASVSCIYGMGNPTEFENGIIRI
                     KKGQTISRQGFLHNLVNVLYHRSQGDFSRGTFRVNGDTVDINLPYVDYGIRITFFGNE
                     IEEIESLEISSSKKISAMDDAAIFPANLYLAPKDMIQQVMNDIQDEMMEQVAYFKTQG
                     KLLEAQRIKERVEYDIEMIRELGYCSGIENYSRFFDRRKPGTRPFCLLDYFPKDFVLV
                     IDESHQTIPQVGGMYGGDRSRKMVLVEYGFRLPSALDNRPLNFHEFENQIGQTIFVSA
                     TPGDYELKKTEGIIVEQVVRPTGLLEPPIEVRPSVNQIDDLLEEIDKRVKKNDRVLVT
                     TLTKRMAEEMDKYLQRINIKSKYIHSDVDALERVEILRQLRLGEIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNERSLTQTAGRAARNVNGLVIFYADKITQSMQRTIDET
                     NRRREKQIAYNVMHNITPKTVSKSIQQIMQQTSVLDIKGFDETKQYAIIDGDEMFNSA
                     AEEKRTYKTIPQMEKAIASLKKKMEKAAKDLDFIEAAKLRDEMFRMEKELQSMK"
     gene            complement(3423..5294)
                     /gene="htpG"
                     /locus_tag="PLY81_04325"
     CDS             complement(3423..5294)
                     /gene="htpG"
                     /locus_tag="PLY81_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNF29484.1"
                     /translation="MQKGQIRVQTENIFPIIKKFLYSEHDIFLRELISNATDATQKLK
                     TLSSIGEAKGELGDLRIDVVLNAKEKTICFEDRGVGMTKEEVDKYLNQVAFSGAEEFL
                     QKYKDASIIGHFGLGFYSAFMVAEKVEVITQSHNADAATVYWSCDGSPEFELYEVEKK
                     QRGTKIILHINDESKEFLEAHKLKEILNKFCKYLPIPIYFTDIAEEKKEGEEEKSINN
                     TNPIWVKKPSDLTKEDYENFYRELYPFGETPLFWIHLNVDYPFNLTGILYFPKIKQSY
                     EIQKDKIQLYSNQVFVTDEVKDIVPEFLMLLHGVIDSPDIPLNVSRSYLQGDPNVKKI
                     NNHITKKVADKLEEIFNKERTEFEQKWESLGLFVKYGMMTDDKFLEKANKFLLLENTT
                     ENKFYTLEEYKTTTEALQKNKEGKQVILYTTNPIQQDAYIQAAQAKGYNVVKMETIVD
                     AAFINNMEMKWENVTFTRVDADITDNLIDKQDTTESVLSKNEEEELKKLFEIKENEIT
                     VSVEIKGLQKDSQPVIATRPEFMRRMKDMASMGGGMSAFYAQMPDEVTLTVNGNHPIY
                     QTLLKESDIEKKEKQVRNLADLALLSQGLLKGAALTNFINRSVYLMQNEETKIAATV"
     gene            5449..>6198
                     /locus_tag="PLY81_04330"
     CDS             5449..>6198
                     /locus_tag="PLY81_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29485.1"
                     /translation="MNSSKLIVLITCFVFLGCALFFINNNFWQDEIYTLIHFVFVPFK
                     TTLTNYHNANNHILFSLVLNIHKQIAGINNLQQALLNPVYFRIIPFIISTFSFIIFYT
                     ITAKIYGKQIALIASSVFASTFALLNFSVQLRGYSLSTFLAIIQTYYFLKIIKNQVVL
                     KTDFIKLFIATSLCIFCLPVNVYYATAMILLAIIIYVFVAYKKYNFSTQISKHNIFYL
                     ILSLLFAIVLNILYYYWLFSFSVPTENNLPVN"
BASE COUNT         1836 a         1058 c          800 g         2504 t
ORIGIN      
        1 ttattgttcc acaaataagt ttgtttatgc cctggcaagc tgtaactgcc aacatctacc
       61 tgtagggtgc ctaattgtat tttagaagcc tgttctatta atgtgtagtt atcatttcca
      121 cttaccaaaa atgtattttg attgctattg tttaaaacat tattaatttc tgtaagactg
      181 atttgttttt ttaaaacggg tgtgttttga gcaactattt gaatgctgat aaatagtgaa
      241 atagtaaatg caagatgtat tttcaaagta tagtttttaa tagtgttaaa gttaaaatct
      301 tagcaggata tctgttctgc aaattaattt gactgaggca aattttctat atcatttaaa
      361 tctttatggc ggccagcagc ttttttggct tgtaaaagtt gatttaaatg aataaccctg
      421 atttccacat tctctacttt ttgaattgtt gcatgtgcaa aagcttcatc aaatgcaact
      481 ccttttaatg cagtcataat ttctatagca taaggtgggc gaccaaaaga aaacacatca
      541 tagtcttctg aaaaaaattt ttctttaggt attgcagcaa gcggcaaacc aaattcagta
      601 attgcttttt gaagttgggt aaaattgggt tctgttttat taacccaaat atccatgtcg
      661 cctgtacttc tgctataacc ccttaaaata acagcatatc caccaacaag catatattca
      721 acatcgtgtt tgttgaggct ttttataaaa tctgcaaaat ctgcattaaa aatctccatt
      781 tactttttgt gctttctgct ggtaaaaatc gtacgttcta cttttttctt taaaaaatct
      841 ggttcgccaa aagctgctgc tgttacaatt gcagcagcct gcattttttc ttctatggat
      901 ttatcatgcc aatagttatt atcaatattg ccatcttttg tcatgtttct ggcttcaaaa
      961 actgttctat ctaaaaaact ggttttcatt atgtgcaatt tacaaagatt ttaaataatt
     1021 tacatttatt tcatcccctc caacttcttt ttaaagcaaa atagattcct cattacattc
     1081 ggaatgacat ttattttttt tcatagcaaa acagattcct cattacattc ggaatgacat
     1141 ttattttttt tcaaagcaaa atatattcct cattacattc ggaatgacat ttatttctat
     1201 ttcatacttt gtaactcctt ttccattcta aacatctcat ctcttagctt agctgcttct
     1261 ataaaatcta aatcttttgc tgctttttcc attttctttt ttaaagatgc aatggctttt
     1321 tccatttgtg gaatagtttt ataagttctt ttttcttctg ctgcactatt aaacatttca
     1381 tctccatcta ttattgcata ctgtttagtt tcgtcaaagc ctttaatatc taatactgat
     1441 gtttgctgca taatttgttg aatgctttta cttacggttt ttggtgtaat gttatgcatt
     1501 acattataag ctatttgttt ttctcttctt ctattggttt catcaatggt gcgttgcatg
     1561 ctttgtgtaa ttttatctgc ataaaagatt accaagccgt ttacatttct tgcagcacgt
     1621 cctgctgttt gtgtaaggct tctctcattt cttaaaaagc cttctttgtc tgcatctaaa
     1681 atggcaacta aacttacttc aggtaaatct aatccttctc ttaataaatt tactccaact
     1741 aaaacatcta tttctcccaa acgcaactgt cgcaatattt ctacacgttc taaagcatcc
     1801 acatcgctat gaatgtattt tgattttatg ttgatgcgtt gtaaatattt atccatttct
     1861 tctgccattc ttttagtaag tgttgttact aaaaccctgt catttttttt aacacgttta
     1921 tctatttctt ctaacaaatc atctatttgg tttacacttg gtcttacttc aataggaggt
     1981 tcaagtaaac ctgttggcct tactacttgt tctactataa ttccttctgt tttttttaat
     2041 tcatagtcgc caggtgtggc acttacaaaa atggtttgtc caatttggtt ctcaaattca
     2101 tgaaaattca aaggcctgtt atctaaggca gatggcaatc taaatccata ttctaccaat
     2161 accatttttc tacttctatc gcctccatac attcctccaa cttgaggtat ggtttgatgg
     2221 ctttcatcaa ttaccaatac aaaatcttta ggaaaataat ctaacaaaca aaatggtctg
     2281 gttcctggtt ttcttctatc aaaaaatctc gaataatttt caataccact gcagtaacct
     2341 aattctctaa tcatttctat atcgtactcc actctttctt ttatacgttg tgcttctaat
     2401 aattttcctt gagttttaaa ataagctact tgctccatca tttcatcttg tatatcattc
     2461 atcacttgct gaatcatatc tttaggtgca aggtataagt tggcaggaaa aatggctgca
     2521 tcatccatag ctgaaatttt tttagaagaa cttatttcaa ggctttcaat ttcttctatt
     2581 tcgttgccaa aaaatgttat tcttatacca taatctacat aaggtaaatt aatatctact
     2641 gtatctccat ttactctgaa agttcctctg ctaaaatctc cctggctacg atgatacaaa
     2701 acatttacaa ggttatgtaa aaaaccttgg cgagaaatgg tttgcccttt ttttattctg
     2761 attattccat tttcaaactc tgttgggttg cccataccat aaatgcaact tacactggca
     2821 acaataatta catctcttct gccacttaat aattggctgg ttgcttgtaa ccgaagtttg
     2881 tctaactctt cattaatact taaatctttt tctataaaaa cattgcttac aggcatataa
     2941 gcttctggct gataataatc gtagtagctt acaaaatatc ctactgcatt gtctggaaaa
     3001 aattgcttaa actcgccata taactgagct actaatgttt tgttatgagt tagcactaaa
     3061 gttggccgct gaattttttc gataacattg gcaatggtaa aagttttgcc actgccagtt
     3121 acacccaata gtgtttgaaa ttgttctcca ttatttacac catccaccaa ttgctgaatt
     3181 gctgttggtt ggtcgccact tggttgatag gaagtgtgta atttaaaagt catggtgtaa
     3241 aatacttaaa aggaattagt ttaaataaat aaatttacta tgatgcaact ctttacataa
     3301 aaaagcccaa taataatatt aacaaaatta ttgggctgaa aaattaaaat ataaaggttg
     3361 ctttaagttt ttactacaaa agattttaaa acaatcttga ataaataatg gttaaaacaa
     3421 tattacacag ttgctgctat tttggtctct tcattctgca ttaaataaac acttctgttt
     3481 ataaaatttg taagtgcagc accttttagt aagccttgag aaagtaatgc taaatctgcc
     3541 aaattacgaa cttgtttttc ttttttctct atatcacttt cttttaataa ggtttgataa
     3601 ataggatgat tgccatttac tgttaaggtt acttcatcgg gcatttgagc ataaaaagct
     3661 gacattccac ctcccatact tgccatatct ttcattctac gcataaactc tggtctggtg
     3721 gcaattactg gttgagaatc tttttgtaag ccttttattt caacacttac agttatctca
     3781 ttttctttta tttcaaataa tttttttagt tcttcttctt catttttaga taaaacactt
     3841 tctgttgtat cttgcttatc aattaaatta tctgttatat ctgcatcaac tcttgtaaat
     3901 gttacattct cccatttcat ttccatgtta ttaataaagg cagcatctac aatagtttcc
     3961 atttttacaa cattatatcc ttttgcttgt gcagcttgta tataagcatc ttgttgaatt
     4021 gggttggttg tataaagtat tacctgttta ccttctttat ttttttgtaa ggcttctgta
     4081 gttgttttgt attcttctaa agtataaaat ttattttctg ttgtgttttc taataataaa
     4141 aatttattgg ctttttctaa aaatttatca tcggtcatca ttccatattt tacaaataag
     4201 ccaagacttt cccatttttg ctcaaactca gttctttctt tattaaaaat ttcttctaat
     4261 ttatctgcta cttttttggt gatatggtta ttaatttttt taacattggg gtcgccttgt
     4321 aaatagcttc tactaacgtt aagaggtata tctgggctat ctattacacc atgcaacaac
     4381 attaaaaatt ctggaacaat atcttttact tcatcagtta caaaaacctg gttgctgtat
     4441 aattgaattt tatctttctg tatttcgtag ctctgcttaa tttttggaaa atataaaata
     4501 ccagtaaggt taaaaggata atctacattt aaatgaatcc aaaataaagg tgtttctcca
     4561 aatggataca gttcacgata aaaattttca taatcttctt tagttaaatc gcttggcttt
     4621 tttacccaaa ttggattggt attattgata cttttttctt cttcaccttc tttcttttct
     4681 tcagctatat cggtaaaata aattggtatt ggtaagtatt tacaaaattt atttaaaatt
     4741 tcttttagtt tatgagcttc taaaaattct ttgctttcat cgttaatatg aagaataatt
     4801 ttggtgcctc tttgtttttt ttctacttcg tataattcaa attctgggct gccatcacaa
     4861 ctccagtaaa ctgttgcagc atctgcatta tggctttgtg taattacttc tactttttct
     4921 gcaaccataa atgcactata aaaacctaaa ccaaaatgcc caataatgct tgcatcttta
     4981 tatttctgta aaaattcttc tgcaccgcta aaggcaacct gattaaggta tttatctact
     5041 tcctctttag tcattccaac accacggtct tcaaaacaaa ttgttttttc ttttgcattt
     5101 aatacaacgt ctattcttaa atcaccaagt tcgccttttg cttctccaat ggaagataaa
     5161 gtttttagtt tctgagtagc atcggttgca ttgcttatta attctcttaa aaaaatatcg
     5221 tgttcgctgt acaaaaattt cttaataatt ggaaaaatgt tttctgtttg tacacgaatc
     5281 tgtccttttt gcattgtata agtactttta atggttaaaa taactaatta agcccatttt
     5341 aggctgacct acacagaaaa tcaaacaaaa taccaaattg ttttttctga caggttggca
     5401 attttttaat tggagttatt tacttttaat aattattgaa aagatataat gaatagtagt
     5461 aaactaatag tattaataac ttgctttgtt tttttaggct gtgctttgtt ttttattaat
     5521 aataattttt ggcaagatga aatttatact ctgattcatt ttgtatttgt tccttttaaa
     5581 actaccttaa ccaattacca caatgctaat aatcatattt tatttagttt agttttaaat
     5641 attcataagc aaattgctgg aataaataat ttgcaacagg cattattaaa ccctgtttat
     5701 ttcagaatta ttcctttcat aatttcaact ttttctttta ttatttttta taccattact
     5761 gctaaaattt atggcaaaca aattgcctta attgcatcat ctgtttttgc ttccactttt
     5821 gcattgctta atttttctgt tcagttacgt ggttactctc taagtacatt tttagctatt
     5881 atacaaacgt attatttttt aaaaatcatt aaaaatcagg ttgtacttaa aaccgatttt
     5941 ataaaattat ttattgctac aagcttatgt attttttgtt tacctgttaa tgtatattat
     6001 gctacagcaa tgattttgtt ggcaattatt atttatgttt ttgttgcata caaaaaatat
     6061 aatttcagca ctcaaatatc aaagcataat attttttatt taatactatc tttattgttt
     6121 gcaattgtat taaatatact ctactattat tggttattta gtttttctgt tccaacagaa
     6181 aataatttac cagttaac
//
LOCUS       DAOQHP010000094         6191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6909_length_6191_cov_4.221969, whole genome shotgun sequence.
ACCESSION   DAOQHP010000094 DAOQHP010000000
VERSION     DAOQHP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6909_length_6191_cov_4.221969"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="PLY81_04335"
     CDS             complement(<1..1847)
                     /locus_tag="PLY81_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNF29486.1"
                     /translation="MSTPLQILQQYWHHQTFRGEQEKIIEAVLNKNDVVALLPTGGGK
                     SVCFQVPAMLMDGLCLVISPLIALMKDQVENLTKKNIPAAAIYSGMSFFEINKILQSA
                     TDNNLKFLYISPERINTKLFQQCIHQLNICLVAVDEAHCISQWGYDFRPSYLKIAKIK
                     EQLNSVPFIALTATATTVVKNDIIDKLQLKNVIVFKQSFEKSNISFSVFNVDSKINKA
                     VEILNKVQGSSIIYCKNRNETKAIAEQLLKQNISANFYHAGLTQEERSVRQNNWIKNK
                     SRVIVCTNAFGMGIDKPDVKTVIHFNIPDCIENYYQEAGRAGRNGSKAYAVLLYQLSD
                     EIELDTLAEVKFPAIETIKEVYQHIANYLQIPIGIGEGNYYNFDIIDFCNNFKLDVVQ
                     VINILKYLEQEGFFAFSENIFIPAKVVFTSNKQTLNSVENRYPHLDLVMKTLLRTYEG
                     IYENNVSINEKLIAKIARLSYDTVFSNLNTLHTLGIINYMPQKETPQLYYMLNRAPAN
                     GLFINYSTYLQRKKLYKEKIEAIKQYVHLAETCRSKFIANYFGDKNVSNCNCCDNCLN
                     LKKNKLSLKEFDQIEKLIYRVLSNNELPLNTLLQQLSNFKKEKVLQVIT"
     gene            complement(1895..2905)
                     /locus_tag="PLY81_04340"
     CDS             complement(1895..2905)
                     /locus_tag="PLY81_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNF29487.1"
                     /translation="MKLVSYLKDGHDQLAFLVDNFLFDCDMVHPELPGNMNMFLHYWD
                     DMYPIAQKVDAAIKAGSISKDRGIDISTIQIIAPIPFPTSCRDGYAFRQHVAAARRNR
                     KVDMIAEFDQYPIFYFTNHLSIQGEGNIYCMPDHFEKLDFELEAAIVICRPGRNIPAE
                     EADKYIGGLMIMNDMSARRLQMEEMLLNLGPAKGKDFCTVIGPCLVTLDELEAFEIPA
                     KENHVGKSWNLKMKCWVNGIQVSEGNVGDMDWTFAEIIERCSYGVNLHPGDVIGSGTV
                     GTGCFLELNGTGKLNNPNYQEQWLQENDVVEMEIEQIGLLKNTIVKEESSFSILAKKK
                     LP"
     gene            complement(2978..3259)
                     /locus_tag="PLY81_04345"
     CDS             complement(2978..3259)
                     /locus_tag="PLY81_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29488.1"
                     /translation="MNTSLDILQIENYTKEDLLKFINNLIHHDFEKLVFLLYRIDVAE
                     EKIKNLLSNNHTTNVEELIANAIIERVMEKKISKEKYKQQNHISEEEKW"
     gene            complement(3275..4177)
                     /locus_tag="PLY81_04350"
     CDS             complement(3275..4177)
                     /locus_tag="PLY81_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNF29489.1"
                     /translation="MSKENILESIDKKYADLGENAETYLKGLLQAKPITYWDYIQVDT
                     LHSLQKPRTNYKDEEIFIMYHQVTELVLKMMVHELKQLVFEEHTEEIYIDKVNRLNRY
                     TSMLITSFDVMKYGMNYDDYNIFRSTLTPASGFQCAQFRYIEIFCTRLENLINEEGKK
                     RLGPNPSVEEYFEHIYWKDAGLNRQTGKKSLTLQLFEEKYLSSFIELAKKVQGNTLEE
                     KINKLPNVSEAFTKKMKEFDHLYNVEWPIVHLQTAQHYLDSKGENKAATGGSEWKKYL
                     HPQYQQRKFFPTIWSEEEKRNWGK"
     gene            complement(4263..4334)
                     /locus_tag="PLY81_04355"
     tRNA            complement(4263..4334)
                     /locus_tag="PLY81_04355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4299..4301),aa:Arg,seq:cct)
     gene            complement(4385..>6191)
                     /locus_tag="PLY81_04360"
     CDS             complement(4385..>6191)
                     /locus_tag="PLY81_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF29490.1"
                     /translation="NTTISTFPYHEDFEAGQGNWTTGGNNNDWQYGTPSKSIITGAAS
                     GTKCWIVGKLTGNFYNFGENAWIMSPCYDFSSLTNPQISFSVFWETERKYDGATFQYS
                     VNGGNTWNTLGSTNSNNNCNGVNWFNYSPITSLGVDGWSGTKLPTSGSCNGTGGSDGW
                     VTSKHILTGLAGQNNVRFRFVFGAGTTCNSFNGFAVDDITISEAPVNTCNFIFSCTGN
                     NTVNFTNTSSICATNFNWNFGDNASPSNTSNAENPTHIFTAPGTYNVSLTVTFPGNII
                     VNKIKTVTVLSVNTSIVNSIKCNGDSIGSALAIVTGGGGVYNYNWNTNPVQTTNTATN
                     LKAGSYTVTVSATNTCTTNSTIIFTQPDSLKATSIVANELCKQQNGFIEVVTTGGVLP
                     YTYLWNTNPIQNTSKASNLKAGNYAVIITDANLCKLSMNNSIKDSINNINLNLGNDTS
                     FCPGSQLILNAGAGFANYVWQNASTNATFTATQTGNYFVIVTDKDGCTKTDTIKINVD
                     CSDIYFPTAFTPNSDGLNDTFGALGNLFSLTSYQFYVYGRWGQLIFYTNNAFEKWDGK
                     QNGNNLDSGTYIWFATYSINNKPLLFKKGTVIMIR"
BASE COUNT         1999 a         1043 c          827 g         2322 t
ORIGIN      
        1 aatgtaatta cttgtaaaac tttttctttt ttaaaattag acaactgttg tagcaaagta
       61 tttagtggta attcgttatt ggacaatacc cgatatatta gtttttcaat ttggtcaaat
      121 tctttgagag ataatttgtt ctttttaagg tttaaacaat tatcgcaaca attacaattg
      181 ctcacatttt tatctccaaa atagtttgca ataaatttac ttctacaagt ttctgctaaa
      241 tgtacatatt gctttatggc ttcaattttt tctttatata atttttttct ttgtagataa
      301 gtggaataat tgataaataa tccatttgca ggtgccctat ttaacatata atacaattga
      361 ggtgtttctt tttgtggcat atagttaatt atgcccaatg tatgtaatgt atttaaatta
      421 gaaaatactg tatcgtaaga tagtcttgca atttttgcaa tcaatttttc attaattgaa
      481 acattatttt cataaattcc ttcataagta cgaagcaagg ttttcattac taaatctaaa
      541 tgagggtatc tgttttcaac actatttaaa gtttgtttat ttgatgtaaa aaccactttt
      601 gcaggtataa aaatattttc tgaaaaagca aaaaaacctt cctgctctaa atattttaaa
      661 atattaatta cttgtacaac atctaattta aaattgttgc aaaaatcaat tatgtcaaaa
      721 ttgtaataat tgccttcgcc aatgccaatt ggtatttgaa gataattagc aatatgctga
      781 tacacttctt taattgtttc aattgcagga aattttacct ctgctaaagt atctaattca
      841 atttcatcgg atagttgata caacaaaaca gcataagctt tactaccatt tctgcctgca
      901 cggccagcct cttgataata attttctata caatctggaa tattaaaatg tataaccgtt
      961 ttaacatctg gtttatcaat acccattcca aaagcatttg tacaaacaat aaccctgctt
     1021 ttatttttta tccaattatt ttgtcttaca ctcctttcct cttgcgttaa accagcatga
     1081 taaaaattgg cagatatatt ttgttttaat aattgttctg ctattgcttt tgtttcattt
     1141 ctgtttttac aataaataat gctacttcct tgtactttat tcaatatttc tactgcttta
     1201 tttattttac tatcaacatt aaaaacagaa aaagaaatat ttgatttttc gaaagactgt
     1261 ttaaaaacta ttacattttt caattgtaat ttgtcaataa tatcgttttt tacaacagta
     1321 gttgcagttg cagttaaagc aataaatggc acactattta attgctcttt aatttttgca
     1381 atttttaaat aggatggcct aaaatcgtag ccccattggc taatacagtg tgcttcatct
     1441 actgcaacta aacaaatatt taattgatga atacattgct gaaataattt tgtatttatt
     1501 ctttcaggtg aaatatataa aaactttaga ttgttatctg tagcactttg aagtatttta
     1561 ttaatttcaa aaaaactcat cccactataa atggcagctg caggtatatt ttttttggtt
     1621 aagttttcaa cctgatcttt cattaatgca ataagaggag aaattactaa gcacaaacca
     1681 tccattagca ttgctggtac ttgaaagcaa acagatttgc caccaccagt tggcaataaa
     1741 gcaacaacat cgtttttatt taaaactgct tcaataattt tttcttgttc gcctctaaat
     1801 gtttgatgat gccaatattg ctgtaatatt tgcaaaggtg tactcatgct tattgcaata
     1861 ttagcaataa gttagctatg tagtttgtac taaattaagg taatttcttt ttggctaaaa
     1921 tagaaaatga gctttcttct tttacaattg tattttttaa taagcctatt tgttcaattt
     1981 ccatttctac tacatcattt tcttgcaacc attgctcctg gtaatttgga ttgtttagtt
     2041 tacctgtgcc attaagttct aaaaaacagc ctgtgccaac tgttccacta ccaattacat
     2101 ctcctgggtg aagatttaca ccataactac aacgttcaat aatttctgca aaagtccaat
     2161 ccatatcacc aacatttcct tcacttactt gaatgccatt tacccaacat ttcattttta
     2221 aattccatga tttaccaaca tggttttctt ttgcaggaat ttcaaatgct tccaactcat
     2281 ctaaagtaac taagcatgga ccaattacgg tacaaaaatc ttttcctttg gcgggcccta
     2341 agtttaacaa catttcttcc atttgcaacc ttcttgcact catgtcgttc ataatcatta
     2401 aaccaccaat atatttatct gcttcttctg caggtatatt tctgccaggg cggcaaataa
     2461 caattgctgc ttctaattca aaatctaatt tctcaaaatg atcaggcata caataaatat
     2521 ttccttcgcc ttgtatgctt aaatgatttg taaaataaaa aatagggtat tgatcaaact
     2581 ctgcaatcat atcaactttt ctatttcttc tggctgctgc aacgtgttgt ctaaaagcat
     2641 atccatctct acaacttgtt ggaaatggta ttggagcaat tatttgtata gtgcttatat
     2701 caatacctct atctttacta atactgcctg cttttattgc tgcatctact ttttgtgcaa
     2761 tagggtacat atcatcccaa taatgtaaaa acatattcat attgcccggt aattcggggt
     2821 gcaccatatc acaatcaaac aaaaaattat ccactaaaaa tgctaattgg tcgtgtccat
     2881 cttttaaata agaaacaagt ttcatggtaa aaatgtttat gttgcgaaag tatattaata
     2941 cacttaaact taataaataa ttgtagtaaa tattagctta ccacttctct tcttctgaga
     3001 tatggttttg ttgtttatat ttttctttag aaatcttttt ttccatcact ctttcaatta
     3061 ttgcattagc aattaattct tccacattag ttgtatgatt attgctcaat aagtttttta
     3121 ttttttcttc tgcaacatca attctgtata gaagaaaaac aagtttttca aaatcgtgat
     3181 gaattaaatt attaataaat tttaataaat cttctttagt gtaattttct atttgtagga
     3241 tatctaaaga agtgttcatt tgaaaaattt aaaattattt tccccaattt ctcttttctt
     3301 cctcactcca aatagttgga aaaaatttac gttgttgata ttgagggtgt aggtattttt
     3361 tccattcgct gccacctgtt gctgctttat tttctccttt actatctaaa taatgctgtg
     3421 cagtttgtaa atgaacaatt ggccattcta cattatacaa atggtcaaat tctttcattt
     3481 ttttagtaaa tgcttcagac acattaggga gtttgtttat tttttcttct aaggtattac
     3541 cctgcacttt tttggcaagc tcaataaaac tagataaata tttttcttcg aaaagttgta
     3601 gagttaaaga tttttttcct gtttgcctgt ttaatcctgc atctttccaa taaatatgtt
     3661 caaaatattc ttcaacagat gggttggggc ctaatctttt cttaccttct tcgttaatta
     3721 aattttctaa cctcgtacaa aatatttcta tatatctaaa ttgagcacac tgaaaaccac
     3781 ttgcaggcgt aagagtactt ctaaatatat tgtaatcatc ataattcatt ccatacttca
     3841 ttacatcaaa agaggtaatt aacatagaag tgtagcggtt taatctgttt actttatcta
     3901 tataaatttc ctcagtatgt tcttcaaata ccaactgctt tagttcatga accatcattt
     3961 taagaactaa ctctgttacc tgatgataca tgataaaaat ttcttcatct ttataatttg
     4021 ttcttggttt ttgtaatgag tgtaaagtat ctacctgtat ataatcccaa taggtaatag
     4081 gttttgcctg taataaccct tttaaataag tttctgcatt ttctcctaaa tcagcatatt
     4141 ttttatctat actttctaaa atattttctt tactcatagc aaacaaatta ataagtttaa
     4201 gttttgatta ttaagaataa aattagggca ataaaagttg ctcttaatat taaaattatg
     4261 agtggtctca ccaagaatcg aacttggatc tagagtttag gaaactccta ttctatccgt
     4321 tgaactatga gacctttaga catacaattt tcaatgagcc taagcaacaa atgtaagtaa
     4381 aaaattatct aatcattatt acagttcctt ttttaaatag taagggttta ttatttatgg
     4441 aataagtggc aaaccaaata taggtgccac tatctaaatt gtttccattt tgtttgccat
     4501 cccatttttc aaaagcattg tttgtataaa aaattaattg cccccaccga ccataaacat
     4561 aaaattgata gcttgttaat gaaaataaat tacccaatgc tccaaatgta tcatttaacc
     4621 catcactatt aggtgtaaat gcagttggaa aataaatatc gctacaatca acattaattt
     4681 ttatggtatc cgtttttgtg cagccatctt tatctgttac aataacaaaa taatttccag
     4741 tttgtgtagc tgtaaatgtt gcattagtag aagcgttttg ccaaacataa tttgcaaatc
     4801 cggcacctgc atttaatatt aattgactac ctgggcaaaa agaagtatcg ttacctaagt
     4861 ttaggtttat attgtttata gaatctttta tagaattatt cattgataat ttacacaaat
     4921 ttgcatctgt aataattact gcataattac ctgcttttaa attgctggct ttagaagtgt
     4981 tttgtatggg attggtattc cataaataag tataaggcaa aacaccacca gtagtaacta
     5041 cctcaataaa accattttgc tgcttacata attcatttgc aacaatagag gttgccttta
     5101 acgaatcggg ctgtgtaaaa ataatagtag aatttgtggt acatgtatta gtagcactta
     5161 cagtaactgt ataacttcct gcctttaaat ttgttgcagt attagttgtt tgaacaggat
     5221 tggtattcca attataatta taaactccac caccaccagt tacaattgca agtgccgatc
     5281 ctatagaatc gccattacat ttaatactat ttactattga tgtattaaca ctaagtacag
     5341 taacagtttt tattttattt acaataatat ttccaggaaa agtaactgtt aaagaaacat
     5401 tatatgtgcc aggtgcagta aaaatatgcg taggattttc agcattactt gtatttgatg
     5461 gagatgcatt atcgccaaaa ttccagttaa aattggttgc acatattgag gaggtattgg
     5521 tgaagttgac tgtgttattg cctgtacatg aaaaaataaa attacatgtg tttaccggtg
     5581 cttctgaaat agtaatatca tctaccgcaa agccattaaa gctattacag gttgttcctg
     5641 caccaaaaac aaatctaaaa cgcacattat tttgccctgc taaacctgtt aaaatatgtt
     5701 tagaggttac ccaaccatcg ctaccacctg taccattaca gctaccagac gttggtaatt
     5761 ttgttccact ccaaccatca acccctaaag aggtaattgg tgagtaatta aaccaattaa
     5821 caccattaca attattatta gaattggttg agcctaatgt attccaggta ttacctccat
     5881 ttacagaata ttgaaaagtt gcaccatcat attttctttc tgtctcccaa aaaacgctaa
     5941 aagaaatttg tgggttggtt aaggagctaa agtcataaca ggggctcata atccatgcat
     6001 tctcaccaaa gttgtaaaag ttacctgtta acttgcctac aatccaacat tttgtaccac
     6061 ttgctgcacc agtaatgata ctttttgatg gagtgccata ttgccaatcg ttattgtttc
     6121 ctccagtagt ccaattccct tgcccagctt caaaatcctc atgataagga aaagtagaaa
     6181 ttgtagtatt a
//
LOCUS       DAOQHP010000095         6166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6957_length_6166_cov_3.840779, whole genome shotgun sequence.
ACCESSION   DAOQHP010000095 DAOQHP010000000
VERSION     DAOQHP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6957_length_6166_cov_3.840779"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLY81_04365"
     CDS             <1..275
                     /locus_tag="PLY81_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HNF29491.1"
                     /translation="NQNAQVRIEDYAAKNAITVPVNTIQTDEKGKFVFIAVEQNGKLI
                     AQKKQIVVGELYNNLIEVKSGLQANEKLITEGFQNIYEGQLLKVNG"
     gene            398..3961
                     /locus_tag="PLY81_04370"
     CDS             398..3961
                     /locus_tag="PLY81_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF29492.1"
                     /translation="MKQFIEGVAQKFKQFKPTSWAIDNRTAVYIITILISLYGITKFN
                     TLPKEQFPDIVVPTISINTIYFGNSPNDMENLVTRPIEKQLKGISGAKVKKIESISQQ
                     DFSLIVVEFDTDVKTEIAKQKVKDAVDKAQTDLPTDLTTQPNVQDFDFSEMPIMYVNV
                     SGDYDGMTLKEYADKMQDKFEELSEINRADIVGAPEREIQINVDQYKMQAAKISFTDI
                     SNAISFENRDISGGLVEVGEMKRSIRVKGQFANALEMQNLIVKNLQGAAVYLKDIAQI
                     VDTVKETESYARLDGKNVVTLNIIKRSGENLIEAATKVKAVVAEMQETGQLPPKDKLK
                     IVITGDQSKQTATSFHELINTIIIGFVLVLLVLMFFMGVNNAFFVALSVPLSIFVAFL
                     FLPVADFIVGTSVTLNFIVLFALLFGLGIIVDDAIVVIENTHRIYANGKVKIERAAKE
                     AAGEVFIPVLAGTLTTLAPFFPLLMWKGIIGKFMIYLPVMLILTLAASLIVAFIINPV
                     FAVSFMKPEGRQYEKPKNQIFKANWFWLFLSFGIILNLASWHGLGNFLIFIPLLAVLE
                     RYVLRDIIHFFQERLLPALMNKYEQLLKWILKGRRPAIALVSLFILFPISLFLLIARG
                     NKSTFFPSGDPNFIYVYLKMPVGTKTSTTDSVTAVLEKRVHKILANEMPEKNGIVESI
                     ITNVAVSANNPRDNNRSTQSNLGRIQVSFVEYEKRHGKTTMPFMEEIRKQMVGIPGAI
                     INVAQEDAGPPTDPPVNIEISGDDFQEISSVASALLFHLDSNRIEGIEGLKLDVDLLN
                     PEISIVVDREKALHEGLSTGQVGMEIRNAVFGKEASKLKDGEDDYKIQIRYADLQRNN
                     ITDIMNMRITFRDMNTMGIKQIPLSAIAKVDYTNTTGAVKRKNTKRTIQIQSNVTDPS
                     LAGPVNIELTKKINDFKSKTNIPGDVTIKQTGQGEQEKETGIFLITALITSLLIIFMI
                     LVLQFNGLSKPFIVLTEIFFSIIGVFIGYAVTGMTIASIMLGVGIVGLAGIVIKNGIL
                     LIEFTDELKHRGYKTREAAIEAGKIRIIPVLLTALATMLGLFPLAVGFNIDFVSLFQH
                     LNPKIFFGGDSVVFWGPLSWTIIFGLIFSFFLTLIMVPSMYILSERLRRPMTRFYGTK
                     FIAILGFTGPIFFVLTGIMFLVKKIQGKKVWMGELKEVAEK"
     gene            4114..>6166
                     /locus_tag="PLY81_04375"
     CDS             4114..>6166
                     /locus_tag="PLY81_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF29493.1"
                     /translation="MNHIPQLITDLGLILGAAAIVTILFKWLKQPLVLGYLIAGFLVG
                     PNLHLTPSIAEVANIKVWAEIGVIFLLFGLGLEFSFKKLMKVGAPASITAIIEVSGMV
                     FLGFMLGKLMGWKQIDSIFLGGVMSISSTTIIMRAFDELGVKGKQFAGLVFGILVVED
                     LIAIVLLVLLTTLSISSTFSGSEMLFSVVKLIFFLALWFVLGIFFVPTILRKAKRLLN
                     DEMLLITSLALCILMVWLAARVGFSPALGAFIMGSILAETVYAEKIEHLLKPVKDLFG
                     AVFFVSVGMLFNPQIVVDYAGIIILMIVVIISGKFFFASLGALLSGQALKTSVQAGFS
                     LTQIGEFSFIIASLGLSLNVTSDFIYPIAIAVSAITTFTTPYLLKLSDPFYNWIVKIL
                     PVNWVKAINRYSNRSQKQSIKNEWQEYVRSWIISSFLLTVLISFVVLLSSYYILPYTR
                     TKLGDNTWATIVGSILALIVMAPFLWNLVIPFKKDKLKELAQIQRFRGSLYVLHFIRI
                     AIAIFLIGFLLDRFFSPKIALTVSIIMFIILIIFSKKIQALYNKLKNQFLLNYNEREA
                     IKITEVQSELAPWDAHIVNFTVDANFNGIGQTLLELGWREDFGINIARIFRGEKIINT
                     PGRYERIFPSDKLSIIGTDEQLMQFSSFMKAEVENKKESTTQVKRNEITLEQLIIIPN
                     SL"
BASE COUNT         2213 a          821 c         1056 g         2076 t
ORIGIN      
        1 ctaatcaaaa tgcacaagtt agaatagaag attatgctgc taaaaatgca attacagtgc
       61 ctgttaacac tatacaaaca gatgaaaaag gcaaatttgt ttttattgca gtagagcaaa
      121 atggtaaatt aattgctcaa aaaaagcaaa ttgttgttgg tgaattatac aacaatttaa
      181 ttgaagtaaa atctggttta caagctaatg aaaaattaat tacagaaggg tttcaaaaca
      241 tttatgaagg gcaattgctt aaagtaaatg gataaaaaat aaatttaaga gaacccaaaa
      301 aacaatttta gaatttgttt actaaacatt attctacatt aaataaaaac ctaccaccta
      361 gttttatttt taaaactaat gtagtaaaac attttgtatg aagcaattta ttgaaggagt
      421 agcccaaaag tttaaacaat ttaaaccaac aagctgggca atagataata gaacagcagt
      481 atatataatt accatactta tttcattata tggtataact aaatttaata ctttacctaa
      541 agaacagttt cctgatattg ttgtacctac cataagtatt aataccattt attttggaaa
      601 ctctccaaat gatatggaaa atttggttac cagaccaatt gaaaaacaat taaaaggtat
      661 aagtggagcc aaagtaaaaa aaatagaaag catatcgcaa caagacttta gtttaattgt
      721 ggtggagttt gatacagatg taaaaacaga aatagcaaaa caaaaagtta aagatgctgt
      781 agataaagct caaacagatt tacctacaga ccttactaca caacccaatg tacaggactt
      841 tgattttagt gaaatgccta tcatgtatgt aaatgtaagt ggagattatg atggtatgac
      901 attaaaagaa tatgccgata aaatgcaaga taaatttgaa gaactttccg aaataaatcg
      961 tgccgatatt gttggtgcac cagaaagaga aattcaaatt aatgttgacc aatacaaaat
     1021 gcaagctgca aaaatatctt ttaccgatat tagcaatgca ataagttttg aaaacagaga
     1081 tattagtgga ggattagtag aagttggaga aatgaaaaga agcattagag ttaaagggca
     1141 atttgctaat gctttagaaa tgcaaaactt aatagttaaa aacttacaag gtgcagcagt
     1201 ttatttaaaa gacattgcac aaattgtaga tactgtaaaa gaaacagaaa gctatgcccg
     1261 tttagatggt aaaaatgtag taacactaaa tatcattaaa cgtagtggcg aaaatttaat
     1321 tgaagcagca actaaagtaa aagcagttgt tgcagaaatg caagaaacag gacaactacc
     1381 tccaaaagat aaattaaaaa ttgtaattac aggagaccaa agtaaacaaa ctgccacttc
     1441 ttttcatgaa cttattaata caattattat tgggtttgtt ttagtgcttt tggtattaat
     1501 gtttttcatg ggtgtaaata atgcattctt tgtggcatta agtgtaccac tttccatatt
     1561 tgtagctttc ttatttttac ctgttgcaga ttttattgtt ggtacaagcg ttactctaaa
     1621 ctttattgtg ctatttgcat tgctatttgg tttaggaatt attgtagatg atgcaattgt
     1681 tgtgatagaa aacacccatc gtatttatgc aaacggaaaa gtaaaaatag aaagagcagc
     1741 caaagaagct gctggcgaag tttttattcc agtattagca ggtacactaa ccaccttagc
     1801 cccattcttt cctttattaa tgtggaaagg aattataggc aagtttatga tttatttacc
     1861 tgtaatgctt attctaactt tagctgcatc gttaatagtt gcttttatca taaaccctgt
     1921 ttttgcagtt agttttatga agccagaagg taggcaatac gaaaaaccca aaaaccaaat
     1981 atttaaagca aattggtttt ggctattttt aagttttggt attatactaa atttagcaag
     2041 ctggcatggg ttaggcaatt tcttaatatt tattccttta ttagcagtac tggaaagata
     2101 tgttttaaga gatattattc acttttttca agaaagatta ttgcctgcat taatgaataa
     2161 atatgaacaa ttattaaaat ggattttaaa aggaagaagg ccagccatag cattagtaag
     2221 tttatttatt ttatttccaa tttcattatt cttattaatt gcaagaggta ataaaagtac
     2281 tttctttcct agcggagatc ctaactttat ttatgtttat cttaaaatgc cagtgggcac
     2341 taaaacctcc actaccgatt ctgttacagc agttctagaa aaaagagtgc ataaaatttt
     2401 agccaatgaa atgcccgaaa aaaatggcat cgtagaaagt ataattacca atgttgcagt
     2461 tagtgccaat aatccaagag ataataacag aagtacgcaa agtaatttag gaagaataca
     2521 agtaagcttt gttgaatacg aaaagaggca tggtaaaacc acaatgccat ttatggagga
     2581 aattagaaaa caaatggttg gtattcctgg tgcaataatt aatgttgcac aagaagatgc
     2641 aggaccgcct acagaccctc cagtaaacat agaaataagt ggcgatgatt ttcaagaaat
     2701 ttcatctgta gcatctgccc tattgtttca cttagacagt aatagaattg aaggtattga
     2761 aggtttgaaa ctagatgtag atttattaaa ccccgaaatt tcaatagtgg tagatagaga
     2821 aaaagcatta catgaaggat taagcacagg gcaagtaggt atggaaatta ggaacgctgt
     2881 ttttggtaaa gaagcaagta aattaaaaga tggtgaagat gattataaaa ttcaaattcg
     2941 ttatgccgat ttacaacgta ataacattac agatattatg aacatgcgta ttacatttag
     3001 agatatgaat acaatgggta ttaaacaaat acctctgagt gcaattgcaa aagtagatta
     3061 caccaatacc acaggtgctg taaaacgcaa aaacactaaa cgtactattc aaattcaatc
     3121 aaatgttact gacccttctt tagctgggcc tgtaaatatt gaattaacta aaaaaataaa
     3181 cgactttaaa tctaaaacaa atattcctgg cgatgttacc attaagcaaa caggacaagg
     3241 tgagcaagaa aaagaaactg gtatattttt aataacagct ttaataacct cgttgttaat
     3301 tatttttatg atattggttt tacaatttaa tggtctaagt aaaccattta tagtattaac
     3361 cgaaattttc tttagtataa ttggtgtatt tattggctat gcagttacag gtatgacaat
     3421 tgcatctatt atgcttggtg taggaattgt tggcttagct ggtattgtta ttaaaaatgg
     3481 aatattatta attgagttta cagatgagtt gaaacacaga ggctataaaa ccagagaggc
     3541 tgctatagaa gctggtaaaa ttagaattat tcctgtttta cttactgcat tggctaccat
     3601 gctaggttta tttccattag cagttggatt taatattgac tttgtttcat tatttcagca
     3661 cctaaatcct aaaatatttt ttggtggaga tagtgtagta ttctggggtc cattaagctg
     3721 gacaattatt ttcggattaa ttttctcttt cttcttaacc ttaataatgg taccaagtat
     3781 gtacatttta agtgaaagat taagaaggcc aatgacacgt ttttatggta caaaattcat
     3841 agccatactt ggctttaccg gaccaatttt ctttgtttta actggtatta tgtttttagt
     3901 taaaaaaata caaggtaaaa aagtatggat gggtgagtta aaagaagtag cagaaaaata
     3961 gataataata aaacaattat aaccaaaacc cattgaataa attcagtggg ttttttaatt
     4021 agaaatattt tcatgaatag caaattacaa aaattaaata aaaaaaatta tcttacatgc
     4081 ttaaagcata ctttttcata agccagccaa aacatgaatc atataccgca attaattaca
     4141 gacttaggtt taattttagg tgcagcagca atagttacca tattatttaa atggttaaag
     4201 caacccttgg tattaggcta tttaattgca ggttttttag ttgggccaaa tttacattta
     4261 acaccctcta ttgcagaagt agcaaacatt aaagtatggg cagaaattgg tgtaatattt
     4321 ttattatttg gcttgggttt agagtttagt tttaaaaaat taatgaaagt aggagcacca
     4381 gcttctatca ctgcaataat tgaagtgtct ggaatggttt ttttggggtt tatgctgggt
     4441 aaactgatgg gctggaaaca aattgatagt atttttttag gtggtgtaat gagcatttct
     4501 tctacaacca ttattatgag agcctttgat gaattgggtg taaaaggaaa gcagtttgca
     4561 ggcttagtgt ttggaatttt agtggtagaa gatttaatag ccatagtgtt gcttgtattg
     4621 ctcactaccc tttctataag tagtacattt tctggcagtg aaatgttatt ttctgtagtt
     4681 aaattaattt tctttttagc attgtggttt gtattgggaa tattttttgt acctacaatt
     4741 ttaagaaaag ccaaaagatt attaaatgat gaaatgcttt tgataacatc tttagcatta
     4801 tgtattttaa tggtttggtt agctgcaaga gttggtttct ctcctgcatt aggagccttt
     4861 attatgggtt ctattttagc agaaacggta tatgcagaaa aaattgaaca cttgctaaaa
     4921 cctgttaaag acttatttgg tgcagtattc tttgtatcgg ttggtatgtt atttaatcca
     4981 caaattgtgg tagattatgc aggcataatt atattaatga ttgtagtaat tataagtggt
     5041 aaatttttct ttgcttcttt gggtgcattg ctttctgggc aagctctaaa aacatctgtt
     5101 caggcagggt ttagccttac acaaattggc gaattttctt ttattattgc aagcctgggc
     5161 ctttctttaa atgtaactag cgattttatt taccctattg caattgcagt atctgctatt
     5221 acaacattta ctacaccata tttattaaaa ctatcagacc ccttctataa ttggattgta
     5281 aaaattttac cagttaactg ggtaaaagca attaacagat atagtaaccg ttctcaaaaa
     5341 caatctatca aaaacgaatg gcaagagtat gttcgttctt ggattattag ttcgttttta
     5401 ttaactgtat taatttcatt tgtagtacta ctatcctctt attatatttt accatataca
     5461 agaacaaaat taggcgataa tacttgggca acaattgttg gttctatttt agcactaatt
     5521 gtaatggcac cttttttatg gaatttagta atacctttca aaaaagataa attaaaagaa
     5581 cttgcccaaa tacaacgctt tagaggtagc ttgtatgtat tacattttat aagaattgcc
     5641 atagccattt ttttgattgg ctttttatta gatagattct tttcgccaaa aattgctctt
     5701 actgtttcta ttattatgtt tataatttta attatttttt caaaaaaaat tcaagcatta
     5761 tataataaat taaaaaatca gtttttactt aattataacg aacgagaggc tattaaaatt
     5821 acagaagttc aatctgaact tgcaccatgg gatgcacata ttgtaaactt tactgttgat
     5881 gctaatttta atggtattgg tcaaactcta ttagaattgg gctggagaga agattttgga
     5941 attaatattg ccagaatttt tagaggagaa aaaataataa acacgcctgg acgttacgaa
     6001 agaatttttc catccgataa attatccatt attggtaccg atgagcaatt aatgcagttt
     6061 agttctttta tgaaagcaga agtggagaat aaaaaggaat ctacaacaca agtaaaaaga
     6121 aatgaaataa ctttagagca attaattatt attcctaatt ctttat
//
LOCUS       DAOQHP010000096         6155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_6979_length_6155_cov_3.771803, whole genome shotgun sequence.
ACCESSION   DAOQHP010000096 DAOQHP010000000
VERSION     DAOQHP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6979_length_6155_cov_3.771803"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..580)
                     /locus_tag="PLY81_04380"
     CDS             complement(92..580)
                     /locus_tag="PLY81_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNF29494.1"
                     /translation="MNIHFTAFKKVCSNRLKLNLFLLTKLPMVFFSGIKLKEFSNEKS
                     ITTVNYKWINQNPFNSIYFAVLSMAAELSTGLLAFGNIYKRKPKVSMLVIKMESFFYK
                     KAVGKISFICKDGLAIEEAIEKAIAYNNATPITCTSIGINEQNEIVAKFIIEWSFKSK
                     PR"
     gene            complement(620..1165)
                     /locus_tag="PLY81_04385"
     CDS             complement(620..1165)
                     /locus_tag="PLY81_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29495.1"
                     /translation="MIQQFSYFIQDVQRMLGKYKIRIIHIWLSRLFWGVFLYRLERGL
                     YLTFGKAYSVMRIILIPLFNLIQAYSNIDIHYKADIRGGLLVLHPSVGIVISAFADIG
                     KNLTLTGGNIIGAREGCKLGELKIGNECTLGANAVVLGPINLGNYINIGASACVVKDC
                     VIDGTSLIGVPAKLIPKQPVI"
     gene            1369..4413
                     /locus_tag="PLY81_04390"
     CDS             1369..4413
                     /locus_tag="PLY81_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29496.1"
                     /translation="MHQHKMKLLIILAIVGIALTGNAQFTKINTDSDIAYKMAKEYWQ
                     KEQYSLAYPIFKNIYTQDNYNSNLKEHIKTESKFYFIICGLQLNDPTIETLAVDFITL
                     EANAQKVGIVSFYLGEYYYRKKDFNTANNYFEKASIENLTNQQIAQMKFHQAYGYFTT
                     QNFVKAKPLFNSIRQLTESPNYIDANYYYGFIMFFEKKYKEALESFKLVEKTETYQNI
                     VPFYIAEIYYFAGERDKAIEYAKTALQKGNQYYKLELTQLIGHSLFEKRMYKEALPYL
                     EEYVTKSKKVRREDLYELAYCYYEAGKWNKAIDGFKQLGGKEDSLAQNSMYLLADAYL
                     KIDDKVGARNAFLFCATNSSNAVQKEVSQFNYAKLSYEQGYLDIAHKEIKSFLKTYPK
                     SVYLNEAKELQIAILANTSNYREAMALIDSLPQQSENIQKIYPKILYGRAVENINDQQ
                     LVVANELLSKLLQVPFNANYIQYTYFWKGEIAYRNGNTNEALDYFTLYLKNPQTNEEI
                     NTNNARYNAAYCFLKKENYTAALELFQKVVLNVSASSSLLIQDAYIRSADCFFMLKNY
                     NQALKMYEVVIEQNLSQADYALYQKAIIAGANNKFTEKINLLQSIAIRYPNSPLITEA
                     YLEVANTYLAEEKWADAIPYLDKVIKDKNATAFHPQAYLNNGIAYFNLTKYANALELF
                     KTLVIKYPNSPESDGAIEYIRKIFIEDQKPGEFISFMRENGKPVSYNEEDSLTFKAAL
                     IKYEAREFENAIRGFAEYLSKFPDGRYNIEANYFSAEINVSKKDYEKALPFYIAVAAK
                     APNIYAERSALQCARIYYFDKKDYLNAEKYFTQVKSLTMQQENKLEAMRGLLRCQYKQ
                     LKWAEALPNAQELLKEKGIAADDKMMANMIVAKNHQINNNLQEAISAYKLVIQIATSE
                     FSAEAQYRIAEIYLLQNKLNDAEKAAFDVIKKYGSYEYWVTKSYLLLGDIYFIQKDYF
                     NAEATFKSIAENATITELKQEAEQKLAKVIEEKNKTNKVEQP"
     gene            4423..>6155
                     /locus_tag="PLY81_04395"
     CDS             4423..>6155
                     /locus_tag="PLY81_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29497.1"
                     /translation="MQKIKNICFIALSFVLLLGNSAYAQKRNTKKINKKTTTKKVVTK
                     KQTAATSKPIKPITTETPKKVNEDTTKPDVVIIYAAFKPSLRNAAKINFTAASPVLDS
                     SKISLKYNVPAQNLFFSYQPVPLKPLALFVDSTNQWVNNQYVKVGYGGYNTPYIETGL
                     SFGNGKSKLTSIYAMHTSSKGSMPFQQFGKTNLNIVGNYIVNNAHELVGSLYYKNNTQ
                     YQYGYQPNTLVFTKKDLKQQFNSIGVVATYKRKMPNQYGITYAPTLHINYFFDAKKAG
                     EFNLIAEMPVTKTFKENFKLNVGLTADITAYKDNIKTLNNNLIFLNAGVQYKLNKVKF
                     NIGLQPAFDNKQVNLLPNITAEAKLKNDKFILVAGFTGKFIKNNYNHLSSYNPWMDQP
                     TFLANTRVLEVSAGFKGSAGQHFTYNASVGYLGFKNMPLFVNNTIDEKSFVMMNDANL
                     QAIKIAGEVGYQIQEKFSFLAGTSIYQFTESANKYAYGILPIEINGSLKWKVLKDLLV
                     KTDVFIWEGPKYLSKNNSTEKLSAAIDINIGAEYSIIPKLNLWLQFNNLFNNKYERWN
                     RYEVLGLNVLAG"
BASE COUNT         2411 a          950 c          867 g         1927 t
ORIGIN      
        1 caacattcct gagaaacaaa aagactcagg ttgaatttga accagggtct gatacttatc
       61 agaccttttt ttattttaaa aactgattat actatctagg tttagattta aagctccact
      121 caattataaa ctttgctaca atttcgtttt gctcgttaat accaatagat gtgcaagtaa
      181 ttggggttgc attattataa gctatagctt tttcaattgc ctcttctatg gccagcccat
      241 ccttacaaat aaaagaaatt ttcccaactg cttttttgta aaaaaaagat tccattttaa
      301 ttactagcat acttacttta ggttttcttt tataaatatt accaaatgca agtaaaccag
      361 tactaagctc ggcagccatg cttaacacgg caaaataaat tgaattaaat gggttctggt
      421 taatccactt gtaatttaca gttgtaatag atttttcatt actaaattct ttcagtttaa
      481 ttccactaaa aaataccatt ggtagtttag taagtaaaaa taaatttaac tttaatctgt
      541 tgctacatac ttttttaaat gcagtaaaat gtatattcac aaatcaattt ttgcttaaaa
      601 tgaactaaaa taaagtagtt tatattacag gttgtttagg tattaacttt gcaggtacac
      661 caatcaaact tgtgccatca ataacacaat ctttaaccac acaagcagaa gctccaatat
      721 tgatataatt acctaaatta attgggccaa gaaccactgc attagcacct aaagtacatt
      781 cattcccaat tttcaactca cctagtttac aaccttctct tgcaccaatt atattaccac
      841 ctgttaaggt taaattttta cctatatctg caaatgcaga aataacaata cctacagatg
      901 gatgtaaaac caataaccct cccctaatat cagctttata atgaatatct atattagaat
      961 atgcttgaat taaattaaac aacggaatta aaataattcg cattacacta tatgcttttc
     1021 caaaagttaa atacaagccc ctttctaacc tatataaaaa aactccccaa aaaagtcggc
     1081 taagccatat atgtataatt ctaattttat actttcccaa cattcgttgt acatcttgta
     1141 taaaataaga aaattgttga atcattgtat tggtatatgt accaaaaata aagtaattac
     1201 tatttatact ttgatttatg gtttataaaa tttatacatt atacagttta tccacaaata
     1261 ataaataatg ggtacaaaag gcacttgtac actttgcata aacaaccgat tttgcacttt
     1321 gttaagaaaa taaaaaacaa tatttgttat tcataagttt attattctat gcatcaacat
     1381 aaaatgaagc tattaattat tttagctatt gtaggtatag ctcttactgg caatgcccaa
     1441 ttcacaaaaa ttaataccga ttctgatatt gcttataaaa tggctaaaga atattggcaa
     1501 aaagagcaat acagccttgc ctatccaatt tttaaaaata tttatactca agataactat
     1561 aatagcaatt taaaagaaca tataaaaacc gaatcaaaat tttactttat tatttgcgga
     1621 ttacagctta acgatccaac cattgaaact ttagcagttg attttattac tttagaagcc
     1681 aatgctcaaa aggtaggcat tgtaagtttt tatttaggcg aatattatta cagaaaaaaa
     1741 gatttcaata cagctaataa ttattttgag aaagcaagca tagaaaacct aaccaaccaa
     1801 caaatagccc aaatgaaatt tcatcaggct tatggttatt ttaccactca aaattttgta
     1861 aaagctaaac ctttatttaa ttcaattagg caacttacag aaagcccaaa ttatatagat
     1921 gcaaattatt actacggctt tataatgttt tttgaaaaga aatataaaga agcattagaa
     1981 agctttaaac tagttgaaaa aacagaaacc tatcaaaaca tagtgccatt ttatattgcc
     2041 gagatttatt attttgctgg agaaagagac aaggctattg aatatgctaa aactgcacta
     2101 caaaaaggaa atcagtatta taaattagaa ttaacacaac ttattgggca ctctttgttt
     2161 gagaaaagaa tgtacaaaga ggcactaccc tacttagaag aatatgtaac aaaatctaaa
     2221 aaagtaagaa gagaagattt atacgaatta gcatactgtt attacgaagc tggtaaatgg
     2281 aataaagcta ttgatggttt taaacaatta ggtggtaaag aagattcttt agctcaaaac
     2341 agcatgtatc ttttagctga tgcctattta aaaattgacg ataaagttgg agcaagaaat
     2401 gcttttttat tttgtgcaac caacagtagt aacgctgtac aaaaagaggt atcgcagttt
     2461 aattatgcta agcttagtta cgagcaaggc tatttagata ttgcccataa agaaataaaa
     2521 tcttttctaa aaacataccc aaaatctgtt tatttaaacg aagcaaaaga actacagata
     2581 gccattttgg caaatacaag taattacaga gaagcaatgg ctttaataga ttctctgccg
     2641 caacaaagtg aaaatattca aaaaatttac cctaaaattt tatatggcag agctgtagaa
     2701 aatattaacg accaacagtt agttgtagct aatgaattat tatcaaaact tttacaagta
     2761 ccatttaatg caaattatat ccaatatact tatttctgga aaggagaaat tgcttataga
     2821 aatggcaaca ccaatgaagc tctagattat tttactttat atttaaaaaa ccctcaaaca
     2881 aacgaagaaa taaatactaa taatgcccgt tacaatgctg catattgttt tttaaagaaa
     2941 gagaattata ccgcagcctt agagttattt caaaaagtag ttctaaatgt ttctgcttcc
     3001 tcttctctcc tcattcaaga tgcttatata agaagtgctg attgtttttt tatgttaaaa
     3061 aactacaacc aggctttaaa aatgtatgaa gtagtaattg agcaaaattt aagccaggca
     3121 gattatgcct tataccaaaa agcaattatt gcaggtgcca ataataagtt tacagaaaaa
     3181 ataaatttac tacaaagtat agcaataaga tatccaaatt ctccattaat aacagaagct
     3241 tatttagaag tagctaacac ttatttggca gaagaaaaat gggcagatgc tattccatat
     3301 ttagataaag taattaaaga taaaaatgca actgcctttc atcctcaagc atacttaaat
     3361 aatggtattg catattttaa cttaacaaaa tatgccaatg cattagagct gttcaaaaca
     3421 ttggttataa aatatccaaa ttctccagaa agtgatggag ccatagaata tattagaaaa
     3481 atatttattg aagaccaaaa accaggagag tttatatcct ttatgagaga aaatggtaaa
     3541 ccagtttctt ataacgaaga agactcctta acctttaaag cagcattaat aaagtacgag
     3601 gcaagagagt ttgaaaatgc aataaggggc tttgcagaat atttatctaa atttcctgat
     3661 ggaaggtaca atatagaagc taattatttt tctgcagaaa taaatgtatc taaaaaagat
     3721 tatgaaaaag ctttgccttt ttatattgcc gttgctgcaa aagcaccaaa tatttatgca
     3781 gaacgtagtg cattgcaatg tgccagaata tattatttcg acaaaaaaga ttatctcaat
     3841 gccgaaaaat attttactca ggttaaaagc cttacaatgc aacaagaaaa taagttagaa
     3901 gcaatgcgtg gcttattacg ttgccagtat aaacaattaa aatgggcaga agcattacca
     3961 aatgcacagg aattgttaaa agaaaaagga attgcagccg atgataaaat gatggcaaat
     4021 atgattgtag ccaaaaacca tcaaatcaac aataacttac aagaagcaat ctctgcatat
     4081 aaattagtga tacaaattgc tacatctgaa tttagtgcag aagctcaata cagaattgca
     4141 gaaatatatt tattacaaaa caaattaaac gatgctgaaa aagcagcttt tgatgtaatt
     4201 aaaaaatatg gctcgtatga gtattgggtt accaaaagct atcttctttt aggagacatt
     4261 tactttattc aaaaagatta ttttaatgca gaagctacct ttaaaagtat tgcagaaaat
     4321 gcaactatta cagagttaaa acaagaagct gaacaaaaat tagctaaagt aattgaagaa
     4381 aaaaataaaa ccaataaagt agaacaacca taaatatcta ttatgcaaaa aattaaaaat
     4441 atttgtttta ttgcactttc ttttgttttg cttttaggca atagtgccta tgcacaaaaa
     4501 agaaatacta aaaaaatcaa taaaaaaact acaaccaaaa aagtagttac caaaaaacaa
     4561 acagcagcca catctaaacc aataaaaccc attactaccg aaacacctaa aaaagtaaat
     4621 gaagatacca ccaagccaga tgtagtaatt atttatgcag cttttaaacc ttctttaaga
     4681 aatgcagcta aaataaattt tactgcagca tcacctgttt tagatagttc aaaaatatcg
     4741 ttgaaatata atgtgcctgc ccaaaaccta tttttctctt accaaccagt acctctaaaa
     4801 cccctagcat tgtttgtaga ttctacaaat caatgggtaa ataatcagta tgttaaggta
     4861 ggttacggtg gatacaatac cccatatatt gaaacaggct taagctttgg taatggcaaa
     4921 tcaaaactta ccagtattta tgctatgcat acttcttcaa aaggcagtat gccctttcaa
     4981 caatttggta aaactaattt aaatattgtt ggaaattata ttgttaataa tgcccatgag
     5041 ttagttggca gcctttatta taaaaacaac acccaatacc aatatggcta tcaacccaat
     5101 actttagttt ttaccaaaaa agatttaaag caacaattta acagtattgg agttgttgca
     5161 acctataaac gtaaaatgcc aaatcaatat ggtattactt atgcaccaac cttacatatc
     5221 aactattttt ttgacgccaa gaaagcaggt gaatttaatt taattgcaga gatgccagtt
     5281 actaaaacct ttaaagaaaa ctttaagtta aatgtgggat taactgcaga tattacagca
     5341 tataaagaca atataaaaac tttaaataat aatttaattt tcttaaatgc tggtgtacaa
     5401 tacaaattaa ataaggtaaa atttaatatt ggcttacaac ctgcctttga taataagcaa
     5461 gtaaatcttt taccaaatat tacagcagaa gcaaaactta aaaacgataa atttatttta
     5521 gttgcaggct ttaccggaaa gttcattaaa aataattaca accatttgtc tagctataac
     5581 ccttggatgg atcagcctac atttcttgcc aatacaagag tcttagaagt tagtgcaggc
     5641 tttaaaggat ctgctggcca gcattttact tacaatgcaa gtgttggtta cttgggcttt
     5701 aaaaacatgc ctttattcgt aaacaatacc attgatgaaa aatcttttgt aatgatgaat
     5761 gatgctaacc ttcaagccat taaaattgct ggagaagttg gttatcaaat tcaagaaaaa
     5821 ttttctttct tggcaggaac aagcatctat cagtttacag aaagtgccaa taaatatgct
     5881 tatggcattt tgccaataga aataaatgga agtttaaaat ggaaagtatt aaaagattta
     5941 ttagtaaaaa cggatgtttt tatttgggaa ggtcctaaat atttatccaa aaataatagt
     6001 accgaaaaac taagtgctgc tatagacatc aatattggtg cagaatacag cataatacct
     6061 aaattaaatt tatggttaca gtttaataat ttatttaata ataaatacga acgttggaac
     6121 cgttatgaag ttttaggttt gaatgtattg gcagg
//
LOCUS       DAOQHP010000097         6119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7053_length_6119_cov_3.936840, whole genome shotgun sequence.
ACCESSION   DAOQHP010000097 DAOQHP010000000
VERSION     DAOQHP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7053_length_6119_cov_3.936840"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2374)
                     /locus_tag="PLY81_04400"
     CDS             complement(<1..2374)
                     /locus_tag="PLY81_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF29498.1"
                     /translation="MPKLFLFIITLLINKNINAQKQDELICRHINNQNGLMYGHLTSI
                     AQDKQGYIWITGDEGLQRYDGYEFINYLHNPTTPYETLPNGRLTKVAVDYQNKLWIGS
                     FSNGFGYYNSFNNTYKLYLPKNQPHLSPNAIGFRSFLFSNDSVTYACSTDGLVKLLNG
                     EIVKVFTTNNSSLQGSLIGRIAKDRNNNIWIGSLGGLNMLTSDENNLYNHTNNKTITA
                     FSNNVLKDNTGNKAAIADLFIDGKNNLWISSWKPELYCYNISTNSFKTIKLPNPKPFI
                     YDNMAQCFVEDNNGNLWIGTSNNGVYKYNYSEDKFTHYIHDRSNNRSIASNSINCMLK
                     DYQGNIWLVSDNNISIFNPSDLPVKIILANTAISATMLAKDKSLWAADNNYLFHFDSS
                     LNLLNKIKHNQDKINNVWKVKESINSDEIFILKEKGLTIYNKMNGSIEPFKDLPILKN
                     NPITDIIELNNGNLFLLRWWWDKNLLYLDRAKRKISAVNVPLFDKNNFEISCAIKKDS
                     INYYLFSKKGLMLLNTSTKMVNTLDSNFHAGPTILINNQFYSATSSSGIRIYNLYSKH
                     ISEISKYNGLPINNTKSIIYAGNNEFWIGGTTGIIKWNQQRNSFNTFRENYGINSDNI
                     AENSLSVMPDGELVFNDGSLKLLASENIKKFPPPKVSIIQCIAGDSELTLEKLNGKVV
                     INHNNNILQFKYGAINYNQNIQYHYKLEGHDKTWKIGNSRIVNYTNLAHGNYVFYVKA
                     ITEDGRWNNEITKVNIYVTQAFYKAIWFYALVAIIVICTILFYYRIKINNL"
     gene            complement(2538..3611)
                     /locus_tag="PLY81_04405"
     CDS             complement(2538..3611)
                     /locus_tag="PLY81_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNF29499.1"
                     /translation="MQFRNFKMVGYVVYGRGCFNQLNEILAPHRKNNAPIIFLVDHFF
                     ECKPLINKIPLQGNDKIIFANVTYEPKTTQVDSIAEQLKNEFGTVSGIVGIGGGSTMD
                     LAKAVSLMMNNPGSSADYQGWDLVKQAGVYKVGIPTLSGTGAEVSRTTVLTGPARKLG
                     MNSDFTPFDQIILDPELTKNAPGNQRFYTGMDCYIHCIESLEGTFLNEFSKSYGEKAL
                     QLCRDIFLGKETWDDECDDKLMMASYAGGMSIAYSQVGVAHAVSYGLSYVLGTKHGIG
                     NCIVMNHLEEYYPQGHKEFKKMVDKGGYEIPNGICSNLTEEQFESMMNVSLSLKPLWE
                     NALGKNWEAIMTKQKLRKLYERL"
     gene            complement(3788..3860)
                     /locus_tag="PLY81_04410"
     tRNA            complement(3788..3860)
                     /locus_tag="PLY81_04410"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3825..3827),aa:Phe,seq:gaa)
     gene            4084..4269
                     /locus_tag="PLY81_04415"
     CDS             4084..4269
                     /locus_tag="PLY81_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNF29500.1"
                     /translation="MPGGSEWIIIILAVLILFGGRKIPEFMRGIGKGIREFNDAKNNV
                     KKEIEDGMNEKDKNSSN"
     gene            4310..5746
                     /gene="gatA"
                     /locus_tag="PLY81_04420"
     CDS             4310..5746
                     /gene="gatA"
                     /locus_tag="PLY81_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNF29501.1"
                     /translation="MFTFSTIKDYHIALLNGNTTCVSTVNHYLQAIKDSYHLNAFLEV
                     FECEAIDIAKKLDLNRQQGMPLGKLHGVVVALKDVIIYKGHRASAASKILENYISIYN
                     ATVVEKLLAQEAIIIGINNCDEFAMGSSNENSAFGNVKNALDTTRVSGGSSGGSAVAV
                     QAGMCMISLGSDTGGSVRQPADFCGIVGLKPTYGRVSRYGLIAYASSFDQIGIFANSV
                     EDAALTLEVIAGEDEFDSTVSSLPVPSYSTLLEQAKDQKFSIAYFKETLDNPALDPEI
                     RDTFYSFIKTLTENGHSVQVVDFEYLEYIVPTYYILTTAEASCNLSRYDGVRFGHQTQ
                     HINATLTDVYKATRSIGFGKEVKRRIMLGAFVLSEGYYDAYFTKAQQVRNLLLQKTNT
                     IFEKFDAIISPTVPTVAWKFGEKSNSATEMYLADIYTVYANLVGIPAISLPLFKHSNK
                     MPFGLQIMVSSNKEVILHSLSKNLIKLK"
     gene            5824..>6119
                     /locus_tag="PLY81_04425"
     CDS             5824..>6119
                     /locus_tag="PLY81_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29502.1"
                     /translation="MQWLLNMIGYPKNIQKTAIVLVGLLVLGFGAVSFTKADIQEYRG
                     RGFYALNDTNSNSLIAELAPKNYYTQNAISVDINPLAVGFMQQYADKQGKWFEK"
BASE COUNT         2044 a          966 c          881 g         2228 t
ORIGIN      
        1 ttaagttgtt tatcttaatt ctgtaataaa ataaaatagt acaaattaca ataattgcaa
       61 ctaaagcata gaaccaaatt gctttgtaaa aagcttgtgt tacatatata ttaactttgg
      121 taatttcatt gttccatcgt ccatcctcag taatagcctt tacataaaaa acataatttc
      181 catgagcaag attagtataa tttacaatcc tgctattacc aattttccat gttttatcat
      241 gcccttctaa cttataatgg tattgaatat tttggttgta attaatagct ccatatttaa
      301 attgaagtat attgttatta tgattaatta caactttacc atttagtttt tcaagtgtta
      361 actccgaatc tccggcaata cattgtatga ttgaaacttt aggtggtgga aattttttta
      421 tattttcaga tgctaataac tttaatgaac catcattaaa aaccaattct ccatctggca
      481 ttactgaaag agaattttcg gcaatattat ctgaatttat accatagttt tctcgaaatg
      541 tattgaatga atttctttgc tgattccatt ttattatacc tgtagtacct ccaatccaaa
      601 attcattatt tccagcataa ataatactct tggtattatt tataggtaat ccattgtatt
      661 tactaatttc agaaatatgt ttgctataaa gattgtaaat cctaattcca gaactagatg
      721 ttgctgaata aaattgatta ttaattagaa tagttggacc agcatgaaaa tttgaatcta
      781 gtgtgtttac catctttgta ctagtattta aaagcattaa tcctttcttt gaaaacaaat
      841 aataattaat tgaatctttt tttattgcac atgaaatttc aaaattattt ttatcaaata
      901 atggaacatt aactgcacta atttttcttt tagctctatc taaatacagc aaatttttat
      961 cccaccacca tcgtaataaa aataggttac cattatttaa ttctataata tccgttattg
     1021 ggttgttttt aagtattggc aaatctttaa atggctcaat actaccattc atcttattat
     1081 aaattgttaa gcccttctct ttcaaaatga atatttcatc actgtttata gattctttta
     1141 ctttccaaac attatttatt ttatcttggt tatgttttat tttatttaaa agatttaaag
     1201 aagaatcaaa atgaaataaa taattattat ctgctgccca taagctttta tcttttgcaa
     1261 gcatagttgc agaaattgcg gtatttgcaa gaattatttt tactggtaaa tcagaagggt
     1321 taaagatgct aatattatta tctgaaacta accaaatatt tccttgataa tcttttagca
     1381 tacaatttat actattgctt gcaatgcttc tattattact tctatcgtga atatagtgtg
     1441 taaacttatc ttcggaataa ttgtatttgt aaactccatt atttgaggtt cctatccaaa
     1501 ggtttccatt attatcttca acaaagcatt gtgccatatt atcataaata aaaggctttg
     1561 ggttgggcag ctttattgtt ttaaaactat tagttgaaat attatagcaa tacaattctg
     1621 gtttccaact actaatccaa agattatttt taccatcaat aaaaagatcg gcaattgcag
     1681 ctttgttacc tgtattgtct tttaaaacat tattggaaaa tgcagtaatt gttttattat
     1741 tggtgtgatt atacaaatta ttttcatcgc ttgttaacat atttaatcca ccaagtgatc
     1801 ctatccaaat attgttattt ctatctttag ctattcttcc aatcaatgat ccttgtagtg
     1861 aggagttgtt tgttgtaaaa acttttacaa tttctccgtt caaaagtttt actagtccat
     1921 ctgtactgca agcataagtt acagagtcgt tactaaataa aaaacttcta aaaccaattg
     1981 catttggcga aaggtgtggt tgatttttgg gtaagtaaag tttataagta ttattgaaag
     2041 aattgtaata accaaaacca ttgctaaaag aaccaatcca taatttgttt tgataatcca
     2101 cagcaacttt tgttaatctt ccgttaggta aggtttcata aggtgttgtt ggattgtgta
     2161 aataattaat aaactcatac ccatcgtaac gttgaagccc ttcatctcct gtaatccaaa
     2221 tataaccttg cttgtcttgt gctatactag ttaagtgccc atacattaac ccattttgat
     2281 tatttatatg cctgcaaatt aattcatctt gcttctgtgc atttatgttt ttattaatta
     2341 acaatgtaat aatgaacaga aatagtttag gcatgagttt attttgctat ataaaactat
     2401 tgtttttttt atgaacttta ttaaagttca acattaaatt cgtttttata tagttagcat
     2461 tgaataaaaa atgcattgac actttctaaa aaactgtatc aatgcattta aagagggttg
     2521 ttatttttat ataactgtta cagcctttcg tatagttttc ttaatttttg ttttgtcata
     2581 attgcttccc aatttttgcc caaagcattc tcccataatg gttttaggct aaggctaaca
     2641 ttcatcatac tttcaaactg ttcttctgtt aaattgctac agatgccatt aggtatttca
     2701 tatcctcctt tgtcaaccat ttttttaaat tctttgtgtc cctgtgggta atactcttct
     2761 aaatggttca ttacaataca atttcctatt ccatgtttgg tgcccaaaac ataacttaaa
     2821 ccatagctta cagcatgtgc aacaccaact tggctatacg ctatactcat acctcctgca
     2881 taacttgcca tcattaattt atcatcacat tcatcatccc atgtttcttt acctaaaaaa
     2941 atgtctctac aaagttgcaa tgccttttca ccataacttt tgctaaactc attcaaaaaa
     3001 gttccttcta aactttcaat gcaatgtata taacaatcca ttcctgtata aaaacgctga
     3061 ttacctggtg catttttagt aagctctggg tcaagtataa tttgatcaaa aggtgtaaag
     3121 tcactattca ttcctaattt tctggcaggg cctgttaaaa ccgttgttct acttacttct
     3181 gcacctgtac cacttaaagt aggaatacca actttatata cacctgcttg ttttactaaa
     3241 tcccagcctt gatagtctgc actactccct gggttgttca tcattaacga aacagctttt
     3301 gctaaatcca ttgtactacc accaccaatg cctacaatac cgctaactgt accaaactca
     3361 ttttttaatt gctctgcaat ggaatctact tgtgtagttt tgggttcgta agtaacattg
     3421 gcaaaaataa ttttatcatt tccttgcaat ggaattttgt tgattaatgg tttgcactca
     3481 aaaaaatgat ctactaaaaa aataatagga gcattgtttt ttctgtgtgg agctaaaatt
     3541 tcatttaact gattaaaaca acctcttcca tacaccacat atccaaccat tttaaaattt
     3601 ctaaattgca taattatttt tttaaataat gaataaacaa aagtaaggtt tgttttaaac
     3661 tatgttttaa aactatcaat tattaaaaaa aattaccaaa atgtaataaa atagtaaaat
     3721 gtataagagc ctctttaagc ttattgaaag aaaaagagag gctattaaaa ataacctctc
     3781 tttaaagtgg tgtgggccgg gatcgaaccg gcgacacaag gattttcagt cctttgctct
     3841 acctactgag ctaccgcacc tgcctcccta attggggttg caaatataat ggcaaatcct
     3901 aattcacaaa aaaataataa ttaatttaca acatactttt ttagaatact taaatgtaat
     3961 gatacaatag tttattattt acataaaatg agtgaaaatt attttgactt tattaagaat
     4021 tttattgttt acctttgtgt cttataaaag agcaacatgt ttatacctaa attacttttg
     4081 ggaatgcccg gtggcagcga atggattatt attattttag ctgttcttat tttatttggc
     4141 ggtagaaaaa tacctgaatt catgcgtggc ataggaaaag gtattcgtga atttaacgat
     4201 gcaaaaaata acgttaaaaa agaaattgaa gacgggatga acgaaaaaga caaaaattca
     4261 tctaactaat ttctggcatt tcaataatta ttaaaattac ttaaagcctt tgtttacttt
     4321 tagtacaatc aaagactatc atattgcttt gcttaatggc aatacaacat gtgtatctac
     4381 tgttaaccat tatttacaag caattaaaga ctcctatcat ctaaatgctt ttttggaagt
     4441 ttttgaatgt gaagctattg atatagcaaa aaaattagat ttaaacagac aacaaggcat
     4501 gcctttagga aaactacatg gtgttgtagt tgctttaaaa gatgtaatta tttataaagg
     4561 gcatagggct tctgctgcat caaaaatatt agaaaattat atttcaattt ataatgctac
     4621 cgttgtagaa aaactattag ctcaagaagc tatcattatt ggcataaaca attgcgatga
     4681 atttgcaatg ggaagcagta atgaaaacag tgcttttgga aatgtaaaaa atgctttaga
     4741 cactacaaga gtttctggag gatcttctgg aggttctgct gtagcagtgc aagctggcat
     4801 gtgcatgatt agtttaggta gcgatacagg tggcagtgtt agacaaccag cagatttttg
     4861 tggaattgta ggtttaaaac caacttatgg cagagtttct aggtatggtt taatagctta
     4921 tgcttcctcc ttcgatcaaa ttggtatttt tgcaaactca gttgaagatg ctgctttaac
     4981 cttagaggta attgcaggag aagatgagtt tgatagtact gtttcttcct taccagttcc
     5041 ttcttattct actttgttag aacaagcaaa agatcaaaaa ttttctatcg cttattttaa
     5101 agaaacctta gataatcctg ctttagaccc agaaatacga gacacttttt actcgtttat
     5161 aaaaacttta acagaaaatg gccatagcgt gcaagttgtt gattttgaat atcttgaata
     5221 tatagttcct acatattata ttttaacaac tgccgaggct agttgcaacc tttctaggta
     5281 cgatggggta aggtttgggc atcaaacaca acatataaat gcaactttga cagacgttta
     5341 caaagctacc agaagtattg gttttggtaa agaagttaaa cgcagaatta tgttaggtgc
     5401 ttttgtactt agtgaaggat attatgatgc atattttact aaggcacaac aagttagaaa
     5461 tttattactt caaaaaacca atacgatttt tgaaaagttc gacgcaatca tttctcctac
     5521 tgtaccaaca gtagcatgga aatttggaga aaaaagcaac tccgctaccg aaatgtacct
     5581 ggctgacatt tatacagtat atgcaaacct tgttggtatt cctgctattt cccttccatt
     5641 atttaaacac tccaataaaa tgccatttgg gttacaaata atggtttcta gtaataaaga
     5701 agtaattttg cactccctta gtaaaaattt aataaagtta aagtaagctt tacaacaatt
     5761 tttaaaggaa gttttcaacc acactaactc ttatttttta tcatttaaac aagtgaatag
     5821 attatgcagt ggttattaaa tatgataggt tatccaaaaa atatacaaaa aactgctatt
     5881 gttttagtag gacttttggt tttgggtttt ggtgcagttt cttttacaaa agcagatatt
     5941 caagaatata gaggcagggg attttatgca cttaacgaca ctaactcaaa ttcattaata
     6001 gcagaactag ctcctaaaaa ttattacaca caaaatgcta taagtgtaga tataaatcct
     6061 ttagcagtag ggtttatgca gcaatatgct gacaaacaag gtaaatggtt tgaaaagat
//
LOCUS       DAOQHP010000098         6084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7115_length_6084_cov_3.525129, whole genome shotgun sequence.
ACCESSION   DAOQHP010000098 DAOQHP010000000
VERSION     DAOQHP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_6084_cov_3.525129"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..951
                     /locus_tag="PLY81_04430"
     CDS             493..951
                     /locus_tag="PLY81_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF29503.1"
                     /translation="MALFNIRVYGILKDENNNVLLSDEYIYGNYFTKFPGGGLEFGEG
                     TRECLIREFKEETGLDITVGKHIYTTDFFQISAFNNKDQIISIYYYVHASNLSALKTS
                     ATPFNFKDEQLIDKKGEVESFRFIELNKLTTNDVTLPIDKVVVEIIKQAI"
     gene            1043..2131
                     /locus_tag="PLY81_04435"
     CDS             1043..2131
                     /locus_tag="PLY81_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29504.1"
                     /translation="MQENNTYKIVQIIDVLDAGGAEKVLITLSNLLHKHGHKVTVITT
                     VRKGIIANQLQEGIEIKELNRKHKWHLKSMQQLVQWCKHADIVHVHSAHNLRFLFVAA
                     AFFGLRKKIFFHEHFGNIDVDKRVHWHQKIIFPKVVFIAVSKSLYQWALENLKLKKEN
                     TFLLENIIIKLPIEKIEKIDTDKKHLVMVSNFRPPKNIEFAIELMKFLPSHFHLTILG
                     QPAYTEYLTSIINQIEENSLQHKVTIEHNCSNVQSILPKFDMAIHTANTESGPLALIE
                     YMAQQIPFITYNTGEVVQQIKPHLGELVMNDFDISNWVKKLLAIIENDNSALKEKLKT
                     TFEEFYSEEAYYEKCMAIYKTALKNKKA"
     gene            complement(2165..2899)
                     /locus_tag="PLY81_04440"
     CDS             complement(2165..2899)
                     /locus_tag="PLY81_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNF29505.1"
                     /translation="MELDPTETAIAEPGAFMMMHDGIQLETIFGDGSQQQGGLLGKLF
                     SAGKRMLVGESLFMTAFTNVSSTKKSVSFASPYPGKIVTIDLMRIGGKVICQKDAFLC
                     AAKGVKIGIEFQRKLGTGLFGGEGFIMENLEGDGMAFLHASGFVQEIDLQQGELLKID
                     TGCIVGFTATVDYDIQFVGGIKNTLFGGEGLFFATLKGPGRIWVQSLPISRLAGRILA
                     YGTYKRKEEGSILGGIGNILDGDGWK"
     gene            complement(2974..4764)
                     /locus_tag="PLY81_04445"
     CDS             complement(2974..4764)
                     /locus_tag="PLY81_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNF29506.1"
                     /translation="MNYPNYRLFDAIEYQKNRFPKSDMLNAKEAGTWKHYSTSNIETI
                     VNKFSAGLLKLGVQCNDCTAEGSDKIAIISNNRPEWLFTDFAVQQIGAILVPVYPTTN
                     PLELEFILNDAQIKYVFASSEELYLKAKSVASKVPSVKNIYTFDKVENAPYYLEVLEL
                     ANEELLQNVEKIKKGIPVTHTATIIYTSGTTGTPKGVMLSHENIVSCTFLSKVSFPFE
                     DAPQSKVLSFLPLNHIFEKCVSYIYLFSGISIYYAESLETIGENLKEIQPDGFTTVPR
                     LLEKVYEKIMLKGNELTGIKRKLFFWSVAVASKYDNLKSGGLLYNIKLAIARKLVFKK
                     WREALGGNIQYIVTGGAACQENLLRIFNGAGIPVYEGYGPTENSPVISVNRKEKGGSM
                     YGSVGPIIEGLEVKLAEDGEILVKGPTVMQGYYKRPDLTAEVLKENWLYTGDIGVWVN
                     NKFLKITDRKKELFKTSGGKYVAPQPIENKLKENRFIEQVIIVGAEKKFVGALIVPSF
                     SALSEWMKNNNINVSSNNEAIVNPAVLNLYKEIVEEYNQYFNHVEQVKKFELLPNEWS
                     IDTGEMTPKMSLKRKIILEKYKSSIDRIYN"
     gene            complement(4927..5382)
                     /gene="rpiB"
                     /locus_tag="PLY81_04450"
     CDS             complement(4927..5382)
                     /gene="rpiB"
                     /locus_tag="PLY81_04450"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNF29507.1"
                     /translation="MNPHFNLSLPIAIGCDHAGVDYKIAIKNYLQQKGYVVNDFGTNT
                     KDSVDYPDYAHAVANSVESNLVAFGILLCGSANGVCITANKHQQVRAGLAWNNDVAKL
                     IRQHNNANIICLPARFIALPLALEMIDNFITTAFEGGRHENRVNKIACC"
     gene            complement(5413..>6084)
                     /gene="tatC"
                     /locus_tag="PLY81_04455"
     CDS             complement(5413..>6084)
                     /gene="tatC"
                     /locus_tag="PLY81_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNF29508.1"
                     /translation="FISYRALCNFSHWLGIGDVLCMPPVKVEMQTTTFGGQFISSITI
                     AFVGGFIVAFPYIFWEFWRFIKPALQEKEVKNTRFAIFWVSFFFFTGAAFGFFLLGPF
                     TFNFLAGFQLGTHHVLVTRPTLNDYIDNLVNILLGCGIAFELPVLSYILTKIGIITPS
                     FLKKTRKYAVVLILVAAAVITPSPDWMSQMLVFIPLWMLYEISIIISSRVFKEDLKKE
                     QEEWG"
BASE COUNT         2145 a         1015 c          805 g         2119 t
ORIGIN      
        1 taatctttac atattttaat tgctaaagaa atcttaaagc ctactttttt tatagtttaa
       61 tacttctaag aactaattac attgcaaaaa tgaatactta accctaaaaa ttttgctaac
      121 gttatatcaa cgaattgtta attattaata ttgtaataaa aataaaaaag ttacctatta
      181 aagtgtaacc cattaagcca caaataatta atttagctat tttttattga ttattctgag
      241 tttgcccata ttttctctac taatgcatta aatttagtac aaacctgaca aattgccttt
      301 tctgtcaata tatgtttagc aagtgtataa ctgatttttc aattaaaaaa agtagctgaa
      361 aaaatcattc tataaaaaat ttaaaaaatg gtgtttccaa aatttcaaat actcaaaaag
      421 ataaattgtt attgaaatat gtttaaggtt tagatttgaa caaactagtt ttacacttat
      481 taattttgca caatggcttt atttaatata agagtttatg gcattttgaa agatgaaaat
      541 aataatgttt tattgagtga tgaatatatt tatggaaatt attttacaaa atttcctggt
      601 ggtggtttag agtttggaga aggaacaaga gaatgtttaa taagagaatt taaagaagaa
      661 acagggcttg atattaccgt tggaaagcat atttacacta ctgatttttt tcagatttca
      721 gcatttaaca ataaagatca aattatttct atttactatt atgtgcatgc aagtaatttg
      781 agtgctttaa aaacatcggc tacgccattt aattttaaag atgaacaatt aatagataaa
      841 aaaggtgaag tggagagttt tagatttatt gaattgaaca aattaactac caatgatgta
      901 accttgccta ttgataaagt tgttgtggaa atcatcaaac aagctatata actaatactt
      961 tctttttttc ttttttaaaa aatttattct caactattcc tatctgtgca tttaagtttt
     1021 tctgtacatt cgtaactaaa aagtgcaaga aaataataca tataaaattg tacaaattat
     1081 tgatgtattg gatgctggcg gagcagaaaa agtactgatt acattaagta atttactgca
     1141 taaacatggg cataaagtaa ctgttattac cactgtaaga aaaggtatta ttgcaaatca
     1201 attacaggaa ggtattgaaa taaaagaact taatagaaag cataaatggc acttaaaaag
     1261 tatgcaacaa ttagtgcaat ggtgtaaaca tgctgatatt gtgcatgtac actctgccca
     1321 taacctacgt tttctttttg ttgctgctgc tttttttggt ttacgtaaaa aaatattttt
     1381 tcatgaacac tttggaaata tagatgtaga taaaagagtg cattggcatc aaaaaataat
     1441 atttcctaaa gttgttttta ttgctgtatc taaaagttta taccaatggg ctttagaaaa
     1501 tttaaaactt aaaaaagaaa atacattttt attagaaaat attattataa aactaccaat
     1561 agaaaaaata gaaaaaattg ataccgataa aaagcattta gtaatggtaa gcaattttag
     1621 gccaccaaaa aatattgagt ttgccattga gttgatgaaa tttttaccta gccattttca
     1681 tttaacaata ttaggacaac ctgcctatac agaatatcta acttctatca ttaatcaaat
     1741 tgaagaaaat agtttacagc ataaagtaac cattgaacat aattgtagca acgtacaatc
     1801 tattttacca aaatttgata tggccataca cactgctaat acagaaagtg gcccattggc
     1861 tttaatagaa tatatggcac agcaaatacc ttttattact tataatactg gcgaagtagt
     1921 tcagcaaatt aaaccccact taggcgaatt agttatgaac gattttgata tttctaactg
     1981 ggtaaagaag ttgcttgcaa ttatagaaaa tgataattct gcattaaaag aaaaattaaa
     2041 aacaactttt gaggaatttt attcagaaga ggcttattat gaaaaatgta tggcaattta
     2101 taaaacagct ttaaagaata aaaaagccta attcaaaaaa attgcaataa tgaaagtata
     2161 acaactactt ccaaccatct ccatctaaaa tattaccaat accaccaagt atgctacctt
     2221 cttctttgcg tttataagta ccataagcca aaattctacc agctaaacgg ctaataggta
     2281 aactttgtac ccaaattctt cctgggcctt ttaatgttgc aaaaaataat ccttcgccac
     2341 caaataaagt atttttaata cctcctacaa actgtatatc ataatcaacg gttgctgtaa
     2401 aaccaacaat acagccagta tctattttta gtaattcacc ttgttgtaaa tctatttctt
     2461 gtacaaaccc actggcatgt aaaaaagcca ttccatcgcc ttctaaattt tccataataa
     2521 aaccttcacc accaaataaa cctgtaccta attttctttg aaattcaata ccaattttaa
     2581 ctccttttgc agcacataaa aatgcatctt tttggcaaat aactttacca cctattcgca
     2641 ttaaatcaat ggttacaatt ttaccaggat aaggagaagc aaaacttaca ctttttttag
     2701 tactgcttac attggtaaat gcagtcataa acaaactttc gcccaccaac attcgtttac
     2761 ctgcactaaa taatttacct aataaaccac cttgttgttg actgccatcg ccaaaaatgg
     2821 tttctaattg tatgccatca tgcatcatca taaatgcacc tggctctgca attgctgttt
     2881 cagtggggtc taattcaatt tatacatatt gcatttcttc cccaaaaatt ctgtaatcaa
     2941 tttcatgatt gcctctcata aaaaatattt taattaatta taaattctgt caatagatga
     3001 tttgtatttt tctaaaataa tttttcgttt taagctcatt ttaggagtca tttcaccagt
     3061 gtcaatactc cattcattgg gtaataattc aaatttttta acctgttcaa catgattaaa
     3121 atattggtta tactcttcta caatttcttt atacaaattt aaaacagctg ggtttacaat
     3181 tgcttcgtta tttgatgaaa cattaatatt attatttttc atccattcac ttaatgcaga
     3241 gaatgaagga acaattaagg cacctacaaa ttttttctcg gcacctacaa taattacttg
     3301 ttcaataaat ctattctctt ttaatttatt ttctattggt tgaggggcaa catattttcc
     3361 tccacttgtt ttaaatagct ctttttttct atctgttatt tttaagaatt tattatttac
     3421 ccaaactcct atatcacctg tgtatagcca attttctttt aaaacttcag ccgttaaatc
     3481 ggggcgtttg taatagcctt gcataactgt tgggcctttt actaatattt ctccatcttc
     3541 ggcaagtttt acttctaatc cttcaataat tggacccact gagccataca ttgagcctcc
     3601 cttttctttt ctgttaacac taataacagg gctgttttct gtagggccat atccttcata
     3661 aacaggaatg ccagcaccat taaatattct taataaattt tcctgacaag ctgcaccacc
     3721 agtaacaatg tattgaatat tgccacctaa tgcttctctc cattttttaa aaactaattt
     3781 acgagctata gctaatttta tattgtacaa aaggcctcca ctttttaagt tatcgtattt
     3841 agatgctact gccacactcc aaaaaaataa ttttctttta atgcctgtaa gttcatttcc
     3901 ttttaacatt attttctcgt acactttttc taatagcctt ggcacagtag taaaaccatc
     3961 tggttgaatt tcttttaaat tttcgccaat ggtttctaaa ctttcagcat aataaatact
     4021 aattccgcta aataaataaa tataacttac acatttttca aaaatatggt taagtggtaa
     4081 aaagcttagt acttttgatt gtggtgcatc ttcgaaagga aaacttactt tagataaaaa
     4141 tgtacaactt acaatatttt cgtggctaag cataacccct ttgggcgtac ctgttgtgcc
     4201 agatgtataa ataattgttg ctgtatgtgt aacaggaatt ccttttttta ttttttctac
     4261 attttgtaaa agttcttcat tggctagttc taaaacctcc agataataag gtgcattttc
     4321 tactttatca aaagtgtaaa tattttttac agaaggaact ttacttgcta ccgactttgc
     4381 ttttaagtac agttcctcac tactagcaaa aacatatttt atttgggcat cattcaaaat
     4441 aaattctaac tctaaagggt ttgtagtagg gtaaacagga actaaaattg ccccaatttg
     4501 ttgcactgca aaatctgtaa ataaccactc aggcctgttg ttactaatga tagcaatttt
     4561 atcacttcct tcggctgtgc aatcattgca ttgtacccca agttttaaaa gccctgcaga
     4621 gaatttattt acaattgttt ctatattgga tgtgctataa tgcttccaag tgcctgcttc
     4681 tttggcatta agcatatcgc ttttggggaa cctatttttt tgatattcta tagcatcaaa
     4741 taatctgtaa ttagggtaat tcataaggct gagttgataa atgaatgaat taattttcta
     4801 atcgtcaata cctatctaca caatattagc aaaaaagcat tagataaaat aaaccattaa
     4861 gaattttttt atccttaaat aatatttttc aaattatatt actaaaacaa ccatagaaga
     4921 atttgcttag cagcaagcaa ttttattaac cctgttttcg tgtcttccac cttcgaatgc
     4981 agtagtaata aaattatcta tcatttctaa agctaatggt agagcaataa aacgagctgg
     5041 caagcaaatt atatttgcat tgttatgttg ccttataagt tttgcaacat cattattcca
     5101 tgctaggcct gcacgaactt gttggtgttt atttgcagta atacaaactc catttgcact
     5161 accacatagc agaatgccga aagctaccaa attactttct acactatttg caactgcatg
     5221 agcataatcg ggataatcca cagaatcttt tgtattggtg ccgaaatcat tcacaacata
     5281 tcctttttgt tgtaaataat ttttaatagc aattttataa tctacacctg catggtcgca
     5341 acctattgca attggtaggg aaagattaaa atgtggattc ataatttttt attttgttat
     5401 caattaaaaa ggttaccccc actcctcttg ttcttttttt aaatcttctt taaaaactct
     5461 tgaagaaata attatactta tttcatacaa catccataaa ggaataaata ctagcatttg
     5521 gctcatccaa tctgggcttg gtgtaataac agcagctgca accaaaatta atacaactgc
     5581 atacttcctt gtttttttaa gaaaagaagg tgttataatt ccaatttttg taagaatgta
     5641 ggacaatact ggcaattcaa atgcaatgcc acaacctagt aaaatattta ctaaattatc
     5701 aatataatca tttagggtag gccttgtaac caatacatga tgtgtaccta attgaaaacc
     5761 tgctaaaaaa ttaaaggtaa aaggacctaa caaaaagaaa ccaaaagctg cacctgtaaa
     5821 gaaaaaaaag gaaacccaaa aaattgcaaa ccttgtattc ttaacttctt tttcttgtaa
     5881 ggctggctta ataaaacgcc aaaactccca aaaaatatac ggaaatgcaa caataaaacc
     5941 accaacaaaa gcaatggtaa tactacttat aaactggcca ccaaaagttg tggtttgcat
     6001 ttctactttt acaggaggca tacacaaaac atctccaatg cccaaccaat gactaaagtt
     6061 gcataaggct ctataactga taaa
//
LOCUS       DAOQHP010000099         6044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7179_length_6044_cov_3.464351, whole genome shotgun sequence.
ACCESSION   DAOQHP010000099 DAOQHP010000000
VERSION     DAOQHP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_6044_cov_3.464351"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2916
                     /locus_tag="PLY81_04460"
     CDS             145..2916
                     /locus_tag="PLY81_04460"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF29509.1"
                     /translation="MKYFFIVFIILICFQKSNAQNASIKGVITDTLNKHNLTNAVISL
                     LHSKDSILYTFTRSDINGNFNLSKINKGKYIVLVTYPTYADYFDTIHILNDNNINLET
                     VKLTPKAHLLADVTVVSKIAAVRMKGDTIVYKADSFKVKQGATVEDLIKLFPGIQVDK
                     NGNITAHGTRVEKVMVDGEEFFGDDPTIATKNLSADMVNEVQVFDKKSDQATFTGVDD
                     GQTSRTINLKLKENAKKGWFGKLEASIAIKDVWNNSLMANSFKGKRKLSFYGIASSNG
                     KTGLNWDESSKYGSQSNTSFSMSDDGSTMYFEGDDFDGMEYFGQGLPKSWSGGINYGN
                     KFNNNEQQVNGSYLYSKVINEGTGYSKSKSLLPDTVFFNNENNKTTGVKDKHGINGTV
                     DWQMDSLLSLKVTVSASKGNSYGNSNYYNEALNEQNQYVNNSIRNKINQGKNKNINYN
                     VLVRKKFKKIGRTLSLSLEQKFNTQNNDGYLYSENNFYNANGSLNFSDTTDQKKLSDN
                     NTNNLKSKIAFTEQLLKSLVVEFSYTYQLNNYTNKVLSYDKNNSGKYEDFNTKFSNNF
                     KFTNATSLIGTMFRYQKNKLNVSFGGNVSLANFKQKDLIKDTTYHYQFNNIYPTAILT
                     YKFNSNKRITINYNGNTKQPSINQLQPVADNNNPLFIQKGNPNLVQEFNHAFAVNYNN
                     YQVLKNRGYGFRIRYNTTYNAIRSSIYTDSLGRSISQYVNLNGNNYFTTNINYNTKVK
                     KLDIYVNASYSFNIGNSISIVNNQTNKNVNKSHQMYVGISKEKENKFNLWLYNSIAYN
                     ISSSSIRSEINTQYWSASLGADLSVYLPWKLELNNNIEAELRQKTELFSGNNNITIWN
                     IYISRKIFKGDKSQLRFTAFDILNQRRNYERDISSNTITETGYQQLSQYFLFSFIWNF
                     NKGGAAITK"
     gene            2924..3691
                     /locus_tag="PLY81_04465"
     CDS             2924..3691
                     /locus_tag="PLY81_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNF29510.1"
                     /translation="MKSILLSFIFYFLFINLPAQQIFINTGKIEFEKQVNLHKVLELS
                     WGDEENDNVWIMNMKKELPKIKSTFYNLYFTQNKTIYKAGKEAPQTAQKIPEWLADND
                     ALENITYTDLATHKTIAKKKVFETTFLLEDSITKINWRITNDTRTIAGIECRKAVGKI
                     FDSVYVIAFYTDLITCTGGPLSFTGLPGMILGVAIPRIHTTLFATKVELANIDETIFA
                     IPKSGKKTSYVNLLKQLKSSMNDWGKEGNLIIWKTLL"
     gene            complement(3698..5098)
                     /locus_tag="PLY81_04470"
     CDS             complement(3698..5098)
                     /locus_tag="PLY81_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNF29511.1"
                     /translation="MVLTICYLIGSITFIIGLKMLSHPASARKGNLVAAAGMAIAIVG
                     TIFLYEEEGKKLGNYFWIFGGLAIGTVVGTLIAKKVKMTAMPEMVSLFNGMGGACAAL
                     ISIIEFQHIAHHLNNNVTANYGLLISIFLGAIIGSVSFAGSMIAWGKLNGTIKDFSYN
                     GQQFINVGLMAVILGIAGYLVFSLSLTNAYWFYIIFVVSLLYGVLFVMPIGGADMPVV
                     ISLLNSFTGVAAACGGFLYDNKVMLTGGILVGAAGTLLTILMCKAMNRSLKNVLLGSF
                     GGNKAGAAGSKEQGSFKEIQLSDAATILAFANKVMIVPGYGLAVAQAQHTCHELEKLL
                     EDKGVEVKYAIHPVAGRMPGHMNVLLAEADVSYDKLLEMEDANEEFRSTDVVLVLGAN
                     DVVNPAAKKDPASPIYGMPILEVEQAKNVIVNKRSMKPGYAGIENDLFFQPKTSMLFG
                     DAKAALQKLITEIKSV"
     gene            complement(5151..5459)
                     /locus_tag="PLY81_04475"
     CDS             complement(5151..5459)
                     /locus_tag="PLY81_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF29512.1"
                     /translation="MENILNFIKEHEQIIYIVVLMIFLGIEVIGKVPSVLHTPLMSGA
                     NAIHGVVIIGAIIVMGKAEADNYLALVLGFLAVILGTLNVVGGFVVTDRMLEMFKKKK
                     "
     gene            complement(5495..>6044)
                     /locus_tag="PLY81_04480"
     CDS             complement(5495..>6044)
                     /locus_tag="PLY81_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HNF29513.1"
                     /translation="VEVFDTRPAVKEEVQSLGAKFIEVEGAADASKAGGYAVEQSDEY
                     KQKQQHKIAESVAKSDVIITTAQIPGKQAPILITQAMLDTMKTGSVIIDLAAATGGNT
                     PKTKNNENFTTENGVLIVGNSNLPCGMPFDASKMYGKNILNFLQLIITNEGALNLNFE
                     DDLVKGTCITHNGSIVHNRLID"
BASE COUNT         2270 a          948 c          844 g         1982 t
ORIGIN      
        1 taaatattaa taaatttaat aacgcaatat ttttaactca gtaaattaaa ccttctttta
       61 atacacatta tttgagttag cccaagaaga tgatttttaa atcttaaata aaactacact
      121 ttaataagta attgaaatag taatatgaaa tattttttta ttgtttttat catattaata
      181 tgctttcaaa aatctaatgc tcaaaatgca agtataaagg gtgttattac agacacttta
      241 aacaaacata atttaactaa tgctgtaatt agtttattac attcaaaaga ttctatttta
      301 tatactttta caagaagcga tataaatgga aattttaacc tctctaaaat taataaaggg
      361 aaatatattg ttttagttac ctatcctact tatgcagatt attttgacac tatccatatt
      421 ttgaatgata acaacatcaa cttagaaaca gttaaattaa cacctaaagc tcatttattg
      481 gctgatgtta ctgttgtaag taaaatagct gctgtgagaa tgaaaggcga taccatagtg
      541 tataaagcag atagctttaa ggttaaacaa ggagctactg tagaagatct tatcaaacta
      601 tttcctggta ttcaggtaga taaaaatgga aacataacag cacatggcac aagagtagaa
      661 aaagtaatgg tagatggtga ggaatttttt ggagatgacc caaccattgc aacaaaaaat
      721 ttaagtgccg atatggtaaa tgaagtacaa gtatttgata aaaaatcgga ccaagcaact
      781 tttacaggtg tggatgatgg ccagactagc cgtaccataa atttaaagtt aaaagaaaat
      841 gcaaagaaag gatggtttgg aaaattagaa gcaagtattg ctattaaaga tgtttggaat
      901 aattctttaa tggctaatag ttttaaaggt aaaagaaaat tatcttttta tggtattgca
      961 agtagtaatg gcaaaacagg tttaaattgg gatgagtcta gtaaatatgg ctctcaatct
     1021 aacacttctt tttctatgag tgatgatgga tctacaatgt attttgaagg agatgatttt
     1081 gatggtatgg aatattttgg gcaaggttta cctaaaagct ggagtggtgg tataaattat
     1141 ggaaataaat ttaataataa tgaacaacaa gttaatggta gttacttgta ttcaaaagtt
     1201 attaatgaag gaacaggtta ttcaaaatca aaatcattat tacccgatac cgttttcttt
     1261 aacaatgaaa acaacaaaac tactggcgta aaagacaagc atggtattaa tggaactgta
     1321 gattggcaaa tggatagctt gctatcttta aaagtaactg tttctgcaag taaaggaaat
     1381 agctatggca acagtaatta ttataacgaa gccttaaatg aacagaacca gtatgtaaac
     1441 aacagtatca gaaacaaaat aaatcagggg aagaataaga atataaatta taatgtattg
     1501 gtaagaaaaa aatttaaaaa aataggaaga acattatctt taagtttaga gcaaaagttc
     1561 aacactcaaa ataatgatgg ttacttatat tctgaaaata atttttataa tgctaatgga
     1621 agtttaaatt tttctgacac tacggaccaa aagaaattat cggataataa tactaataat
     1681 ttaaaatcaa aaattgcatt tacagaacaa cttttaaaaa gtttagttgt tgaatttagc
     1741 tatacttacc agttaaataa ttacaccaac aaagtattaa gttatgataa aaacaattct
     1801 ggaaaatacg aagattttaa tactaaattt agtaacaact ttaagtttac caatgctaca
     1861 agtttaattg gcaccatgtt tagataccaa aaaaataaat taaatgtatc ttttggtgga
     1921 aatgtatctt tagccaactt taaacaaaaa gatttaataa aagatacaac ttaccattac
     1981 caattcaaca atatatatcc taccgctatt ttaacttata aatttaactc taataaaaga
     2041 attaccatta attataatgg aaacaccaag cagccaagta taaaccaact acaacctgtt
     2101 gcagataaca acaacccatt gtttatacaa aaaggcaacc ctaatcttgt tcaggaattt
     2161 aaccatgcat ttgcagttaa ttacaataat tatcaggttt taaaaaacag aggttatggt
     2221 tttagaatta gatacaatac cacttataat gcaattagaa gcagcattta tacagatagt
     2281 ttaggaagaa gcatatcgca atatgtaaac ctaaacggta ataattactt taccaccaat
     2341 attaattaca acactaaagt taaaaagtta gatatttatg ttaatgcaag ttactcgttt
     2401 aatattggca atagtataag tatagtaaac aaccaaacca ataaaaatgt aaataaaagc
     2461 catcaaatgt atgtaggcat ttctaaagaa aaagaaaaca aatttaactt gtggctatac
     2521 aactctattg cttataatat atcctcttct tctatacgtt ctgaaattaa tacccaatat
     2581 tggtcagcaa gtttaggagc agatttgtcg gtttatttac cttggaaact agaactaaat
     2641 aataatattg aagctgagtt aaggcaaaaa actgaattat tttcaggtaa taataatatt
     2701 actatttgga atatttatat ctccagaaaa atttttaaag gcgataaaag tcagctacgt
     2761 tttactgcat ttgatatatt aaatcaaaga agaaattatg aaagagatat tagcagcaat
     2821 actattactg aaactggcta tcagcaacta tctcaatatt ttttattcag ttttatttgg
     2881 aattttaata aaggtggtgc tgcaataaca aaataaaaat tatatgaaat caatactatt
     2941 atcttttatt ttttattttt tgtttataaa cttacctgct cagcaaattt ttattaacac
     3001 tggtaaaatt gaatttgaga aacaagttaa ccttcataaa gtacttgaat taagctgggg
     3061 cgatgaggag aatgacaatg tatggataat gaatatgaaa aaagaattgc ccaaaataaa
     3121 atctactttt tataatttat attttaccca aaataaaaca atatataaag caggtaaaga
     3181 agccccacaa actgcacaga aaataccaga gtggctcgct gataatgatg cattagaaaa
     3241 tattacttat accgatttag caacccataa aacaattgca aagaaaaaag tttttgaaac
     3301 taccttttta ttagaagata gtataactaa aataaattgg cgtataacaa atgatacacg
     3361 aaccattgca ggcattgaat gtagaaaagc agttggtaaa atttttgaca gtgtatatgt
     3421 aattgctttt tatactgatt taataacatg cactggtggg cctttaagct ttacaggttt
     3481 accaggtatg attttaggag ttgcaattcc aagaattcat acaaccttat ttgcaacaaa
     3541 agttgaatta gcaaatattg atgaaacaat atttgccatt cctaaatctg gcaaaaaaac
     3601 aagttatgtt aatttactta agcaattaaa atcatcaatg aacgattggg gtaaagaggg
     3661 taatttaatt atatggaaaa ccctacttta atataaatta aacactttta atttctgtta
     3721 tcaatttttg taaagcagct tttgcatcgc caaaaagcat ggaggtttta ggctgaaaaa
     3781 acaaatcgtt ttcaatgcca gcataacctg gtttcatact tcttttatta acaataacat
     3841 tttttgcttg ttctacttct aaaattggca taccataaat tggcgatgca ggatcttttt
     3901 tagcagccgg atttaccaca tcattagcac ctaaaactaa cactacatcg gtacttctaa
     3961 actcttcatt agcatcttcc atttctaata atttatcata acttacatct gcctctgcta
     4021 ataaaacatt catgtggcct ggcatacgac ctgcaacagg gtgtattgca tatttcactt
     4081 ctacaccttt atcttctaat aatttttcaa gttcgtgaca tgtatgttga gcttgggcaa
     4141 cagccaaacc atagcctggt acaatcatta ctttattggc aaatgccaaa attgttgcag
     4201 catcgctaag ctgtatttct ttgaacgagc cttgttcttt gcttcctgct gcaccagctt
     4261 tgttaccacc aaaagagcct aataacacat tcttcaaact tctattcata gctttacaca
     4321 ttaatatggt aagcaatgta cctgcagcac ctactaaaat acctccagtt agcattactt
     4381 tattgtcata taaaaagcct ccacaagcag ctgcaacacc agtaaaagag ttgagtaaag
     4441 atattactac aggcatatct gcaccaccta ttggcataac aaataaaacg ccatataaaa
     4501 gtgatacaac aaatatgata taaaaccaat aagcattggt taatgaaagt gaaaatacca
     4561 aatacccggc aatacctaaa attactgcca ttaaacctac atttataaac tgttgcccat
     4621 tgtaggaaaa atctttaatt gtaccattaa gtttacccca agcaatcata ctacctgcaa
     4681 aagaaactga accaataatt gcccctaaaa atatacttat taataagcca taattggcag
     4741 taacattatt atttaagtga tgagcaatat gctgaaactc aataatagaa attaatgctg
     4801 cacaagcccc acccatacca ttaaataagc ttaccatttc tggcattgca gtcattttta
     4861 cttttttagc tattaaagta cctaccacag taccaattgc caagcctcca aatatccaaa
     4921 agtaattgcc taattttttc ccttcttctt catataaaaa aatggtgcca acaattgcaa
     4981 ttgccatacc tgcagcagct acaaggtttc cttttctggc acttgcaggg tgcgataaca
     5041 tttttaaacc aataataaaa gtgatacttc ctattaaata acatattgtt aataccataa
     5101 aaattaaaat taaatttctt acaattgatt aatcaaactt tataaaataa tcatttcttt
     5161 ttcttaaaca tttctaacat tctatctgtt acaacaaagc caccaactac atttaaagtt
     5221 cctaaaataa ctgctaaaaa gcctaacact aaagcaaggt aattatctgc ttctgctttt
     5281 cccattacta ttattgcacc aataattaca acaccatgaa tggcattggc accactcatt
     5341 aaaggtgtgt gcaatacact tggcacttta cctattactt caattcctaa aaaaatcatt
     5401 agcacaacta tgtaaatgat ttgctcatgt tctttaataa agtttaagat attttccatt
     5461 tttattttga attaattttt aaaaataaaa taggctaatc aattaaccta ttgtgaacga
     5521 ttgaaccatt atgagtaata catgtgcctt tcactaaatc atcttcaaaa tttaagttaa
     5581 gtgcaccttc atttgtaata attaattgta aaaagttaag aatatttttt ccatacattt
     5641 tgcttgcgtc aaaaggcata ccacaaggta aattactgtt gcctacaatg agaacaccat
     5701 tttctgttgt aaagttttca ttattttttg ttttaggtgt attaccacct gttgctgcag
     5761 ctaaatctat aattacactt cctgtcttca tggtatctaa catggcttgt gtaattagta
     5821 taggtgcttg tttgcctgga atttgtgcag tagtaataat tacatcgctt ttggctacac
     5881 tttcggcaat tttatgttgt tgtttttgtt tgtattcatc cgattgttct acagcataac
     5941 caccagcttt agaagcatct gctgcacctt ctacttctat aaatttagct cctaaacttt
     6001 gcacttcttc tttaactgct ggccttgtat caaaaacttc tact
//
LOCUS       DAOQHP010000100         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7226_length_6010_cov_3.580353, whole genome shotgun sequence.
ACCESSION   DAOQHP010000100 DAOQHP010000000
VERSION     DAOQHP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7226_length_6010_cov_3.580353"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2654)
                     /gene="gcvP"
                     /locus_tag="PLY81_04485"
     CDS             complement(<1..2654)
                     /gene="gcvP"
                     /locus_tag="PLY81_04485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNF29514.1"
                     /translation="MNLFNQQNNEFTNRHIGTTNSTEVAKMLQTIGIESIDELVNKTV
                     PTGIRLNNELNTPPAISEFEYLNELKLVAAKNKVFKTYIGQGYYNTITPSVILRNVFE
                     NPGWYTQYTPYQAEISQGRLESLLNFQTMVCDLTGLPLANASLLDEGTAAAEAMNMLF
                     NAAESDSKKKFFVDEAIFPQTIDVIKTRAIPVQIELVFGDYKNAIIDENYFGAIVQYP
                     NNNGSIEDYSNFIQQAHTVGAGVVMATDLLALTLLKSPGELGADVVVGNAQRFGVPMG
                     FGGPHAGFFAAKEEFKRGMPGRIIGVSIDAQGNRALRMALQTREQHIKREKATSNICT
                     AQALLANMAAMYAVYHGAQGLKDIATRVAMLTQVLNNSLNELGFTNINTCYFDTVIIK
                     ADKNIIQPIAEKNQINFRYFSNGLIGISLDETTTQKDVLDIVYVFAESLGKDEAEIEF
                     DSDLVLTSIPNFAKRTSNYLTHPVFNTHHSESQMMRYIKQLENKDLSLNTSMISLGSC
                     TMKLNAATEMIPVSWAEFGQLHPFVPTNQSEGYQQIIQELSSFLCEITGFDACSLQPN
                     SGAQGEYAGLLTIMGYHIANNNAHKNVVLIPISAHGTNPASAVLAGMKVVVVKSNEDG
                     TIDISDLKAKAEQYKDNLGALMVTYPSTYGVFEEGIKEICSIVHANGGQVYMDGANMN
                     AQVGLTSPGIIGADVCHLNLHKTFAIPHGGGGPGVGPICVKSHLAKYLPGHVSFNNNA
                     VGAVSAAPYGSASILLISYAYIKLLGNVGIKNATKYAILNANYMKARLNNHYSILYTG
                     ANGTCAHEFIVDLRPFKQTAGIEAEDVAKRLIDYGFHAPTMSFPVAGTIMIEPTESED
                     KAELDRFCDALISIREEIAAIEDGRSDK"
     gene            complement(2795..3655)
                     /locus_tag="PLY81_04490"
     CDS             complement(2795..3655)
                     /locus_tag="PLY81_04490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF29515.1"
                     /translation="MEAVKENILLLISTPIYLIIICIELLISNYGSRKLYSLKDTVTN
                     IYLMLLNSIIDLAFRSIYIGVIFTFFYKYRIINNWQSAIWYWLFLLLAEDFLYYWLHR
                     FDHKIRFFWAAHVTHHSSQKMNFTVGFRSSVFQPLYRFIYFIPLPLLGFKPLDIVFMY
                     SATQIWGIFVHTELVRNMGWLEYIFVTPSHHRVHHGSNAKYIDKNMGMFLIIWDKLFG
                     TFQKELDNNEYEPIKYGLTTEITNPNAMNIIFHEWNQIRKDLAQPKLSFKQKWLYIFG
                     KPGYKHHDKS"
     gene            3736..4218
                     /locus_tag="PLY81_04495"
     CDS             3736..4218
                     /locus_tag="PLY81_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF29516.1"
                     /translation="MAIEINENWQKKSAENQKSYKFFLQKADKSKLIKQLPQLHEEAF
                     KTIDCLQCAACCKNYSPRFKTTDIKRISKHLKLKESIFIDTYLTIDTDGDYVLKSAPC
                     SFLGKDNSCTIYEQRPSDCKRFPYTDEDVLLKRTNLTLKNASFCPAVYYVLESLIQKK
                     "
     gene            4652..4855
                     /locus_tag="PLY81_04500"
     CDS             4652..4855
                     /locus_tag="PLY81_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29517.1"
                     /translation="METSVARKVADQTANDFLPLHGTDYVEFYVGNAKQAAHFYKTAF
                     GFQSVAYAGPETGVKDKASYVIS"
     gene            4874..5782
                     /gene="hppD"
                     /locus_tag="PLY81_04505"
                     /pseudo
     CDS             4874..5782
                     /gene="hppD"
                     /locus_tag="PLY81_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
     gene            5806..>6010
                     /locus_tag="PLY81_04510"
     CDS             5806..>6010
                     /locus_tag="PLY81_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29518.1"
                     /translation="MNKIFWKYFIAFGLIIIFKNTNAQKIDSLLKEAEKLEFKLKEVD
                     ALNKYKEVLTIYGDNFKALIKASE"
BASE COUNT         2153 a          919 c          882 g         2056 t
ORIGIN      
        1 aatttatcgc ttctgccatc ttctattgca gcaatttctt ctcttataga gattaaagca
       61 tcacaaaatc tgtctaattc tgctttatct tcactttctg ttggctcaat cattatagtg
      121 cctgcaacag gaaagctcat ggttggtgca tgaaatccat aatcaattaa acgctttgct
      181 acatcttctg cttcaattcc tgctgtttgt ttaaatggtc ttaaatctac aataaactca
      241 tgtgcacaag tgccattcgc accagtatat aaaatagagt agtggttgtt taatctagct
      301 ttcatataat ttgcatttaa aattgcatac ttggtagcat tctttattcc aacatttcct
      361 aataacttaa tatatgcata actaattaat aaaatagaag cactgccata aggtgcagca
      421 gaaacagccc caactgcatt gttattgaat gaaacatgac caggtaagta tttagctaaa
      481 tgagatttaa cacaaatagg accaacacct gggccaccac caccatgtgg tattgcaaaa
      541 gttttatgta aatttaaatg gcaaacatct gcaccaataa ttccaggaga tgttaaaccc
      601 acttgagcat tcatattagc tccatccata tacacttgtc ctccatttgc atgtactata
      661 ctacaaattt cttttatacc ttcttcaaat acaccataag tactagggta agtaaccatt
      721 aatgctccta aattatcttt atattgttct gcttttgctt tcaaatcaga aatgtcgata
      781 gtgccatctt cattactttt tactacaact actttcatac ctgctaaaac tgcactagct
      841 gggtttgtgc catgtgcaga aattggaatt aataccacat ttttatgagc attattatta
      901 gcaatatgat aacccattat agttaataat cctgcatatt ctccttgtgc accactatta
      961 ggttgaaggc tacaagcatc aaagcctgta atttcacata aaaagctgga tagttcctga
     1021 attatttgtt gatacccttc tgactgattg gttggaacaa aaggatgtaa ttgaccaaac
     1081 tctgcccaac taacaggaat catttctgtt gctgcattta atttcatagt acaactacct
     1141 aatgaaatca tgctagtatt aagagataaa tctttgtttt ctaattgttt tatataacgc
     1201 atcatttgac tttcactatg atgggtatta aaaactggat gtgttaaata attagaagtt
     1261 ctttttgcaa aatttggaat agaggttaat accaaatcac tatcaaattc aatttccgct
     1321 tcatcttttc ctaaactttc tgcaaataca tacacaatat ctaaaacgtc tttttgggtg
     1381 gttgtttcat caaggctaat tccaattaat ccgttggaaa aatatctaaa attaatctga
     1441 tttttttctg ctattggttg aataatgttt ttatctgctt taatgataac agtatcaaaa
     1501 taacaagtat tgatattagt aaagcctaac tcatttaatg aattatttaa aacttgtgta
     1561 agcattgcca ctcttgtagc aatatctttt aatccctgtg caccatgata aacagcatac
     1621 atagcagcca tattggctaa taaagcttga gcagtacaaa tattactagt tgctttttct
     1681 ctcttaatat gttgctctct tgtttgtaaa gccatacgta aagctctgtt accttgtgca
     1741 tctatactta ctccaattat tctaccaggc ataccacgtt taaactcttc ttttgctgca
     1801 aaaaaacctg catgtggacc gccaaagccc ataggaacac caaaacgttg tgcattacca
     1861 acaacaacat cagctcctag ctctcctggt gattttaata aagttaatgc taataaatct
     1921 gtagccatta ctacacctgc accaactgta tgagcctgtt gaataaaatt gctataatct
     1981 tctatagagc cattgttatt tggatattgt acaattgcac caaaataatt ttcatcaatt
     2041 attgcatttt tataatctcc aaaaactaat tcaatttgca caggaatagc ccttgttttt
     2101 ataacatcta ttgtttgagg aaatattgct tcgtccacaa aaaatttctt ttttgaatcg
     2161 ctttcagcag cattaaataa catgttcatg gcttctgcag cagcagttcc ttcatctaat
     2221 aatgaagcat tggctaatgg caatcctgtt aaatcgcaaa ccattgtttg aaagtttaat
     2281 aaactttcta atctaccctg agaaatttct gcctgataag gagtatattg tgtgtaccag
     2341 cctggatttt caaaaacatt tcttaaaata acactaggtg taatagtgtt ataataccct
     2401 tgtccaatat aagttttaaa aaccttattt tttgctgcaa ctaatttaag ttcatttaaa
     2461 tattcaaact cgctaattgc aggtggtgta tttaattcgt tgtttaacct aatgccagta
     2521 ggcactgttt tattaactaa ctcatcaata ctttcaatac caattgtttg taacatttta
     2581 gctacttctg tagaatttgt tgttccaata tgcctgttag taaattcgtt attttgctga
     2641 ttaaataagt tcataaaaaa tatattttta aatagtgagg tcttaaaaaa ttgcggcaaa
     2701 tgtacgagtt cctattttat ttttaattga ttgttatagg tatagaaaaa gttgttatta
     2761 aatgtaaaaa aagaaaattt atttctacaa aaaatcaact cttatcatgg tgtttatacc
     2821 ctggtttccc aaaaatataa agccattttt gtttaaagga taatttaggc tgagccaaat
     2881 ctttacgaat ttggttccat tcatgaaaaa taatattcat tgcatttggg ttggtaattt
     2941 cagtagttaa accatattta attggttcat actcattatt atctaattct ttttgaaatg
     3001 taccaaaaag tttatcccaa ataattaaaa acattcccat gtttttatca atgtattttg
     3061 cattgcttcc atgatgtact cgatgatgag atggtgtaac aaatatatac tctaaccaac
     3121 ccatatttct aactaattcg gtatgtacaa aaatgcccca aatttgagta gcagaataca
     3181 taaatacaat gtctaaaggt ttaaaaccaa gtaagggtaa tggaataaaa taaataaacc
     3241 tatatagtgg ctgaaaaaca gatgaacgaa aacctactgt aaaattcatt ttttgagaag
     3301 agtgatgcgt tacatgagct gcccaaaaaa atcttatttt atgatcaaac ctatgtagcc
     3361 aataatataa aaaatcttct gctaataata aaaaaagcca ataccaaata gctgattgcc
     3421 aattattaat tattctgtat ttataaaaaa aagtaaaaat aactccaata taaatgcttc
     3481 taaaagctaa atcaataata ctatttaata gcattaaata aatattggta actgtatctt
     3541 ttaaggaata caattttcta ctaccatagt tagatataag taattcaata caaataatta
     3601 ttaaataaat tggtgtagaa attagtaaga gaatattttc tttaactgcc tccataataa
     3661 aacaaagtaa tcaattttat aataaagtga gaatgaaaat taatgtttaa ctagtttaaa
     3721 taaatacatt tgcctatggc aattgaaata aatgaaaatt ggcagaaaaa aagtgccgaa
     3781 aaccaaaaga gttataaatt ttttttacaa aaagcagata aaagtaagct tataaagcaa
     3841 ctgcctcaat tacatgagga agcttttaaa acaatagatt gtttgcaatg tgctgcatgt
     3901 tgtaaaaact attcaccacg ttttaaaaca actgatatta aaagaattag taagcattta
     3961 aaactaaaag aaagcatttt tatagatact tatctaacaa ttgatactga tggagattat
     4021 gtactaaaat ctgctccttg tagtttttta ggtaaagata attcttgtac tatttacgag
     4081 caacgtccta gcgattgtaa acgatttccc tatactgatg aagatgtgtt attaaaacgt
     4141 accaatttaa cacttaaaaa tgcatcattt tgtccggcag tgtattatgt tttagaaagt
     4201 ctaattcaaa aaaagtaaat tattctattt accagcttca aattgtagat tatatatact
     4261 gattagatgc atctcataaa aaacttggtt tgggttattt atatgctacc tgttataatg
     4321 gaacttaaag tataatagat accactttgc cagtaataat taacctttct gccaattctt
     4381 tataagagaa aataatcaat gtttatttaa tagaaaagaa ggtggaatga caggttcaat
     4441 aacttctact ttaagttact atgagtttac taaaactaga gaaatacttg cagatgagaa
     4501 tgaagaataa tttttttagt tgaattgaca aacttgtata atcatattca gcttttttaa
     4561 gttaatttaa ttatttatcc atgtataata ttcttaaata aactacattt gacacgcttt
     4621 tttaaattat tattttaata ttatttcaat tatggaaaca tcagtagcaa ggaaagttgc
     4681 agatcaaaca gcaaatgatt ttttaccatt gcatggtacc gattatgtag agttttatgt
     4741 aggtaatgcc aaacaagcag ctcacttcta taaaactgct tttggttttc aaagtgtggc
     4801 ttatgctggc ccagaaacag gtgtaaaaga taaagccagc tatgtaatta gttaaaacaa
     4861 gttaactttt gttcttacta ctcctttaaa aacaaataac cccattgcag atcatattta
     4921 taaacatggc gatggtgtaa aggttttggc attaagagta gatgatgcta cagatgcatg
     4981 gcatcaaaca accaaaagag gcggtaaaag ttatttagag cctgttacct taaaagataa
     5041 tgatggagaa gttgtgttaa gtggtattca tacttatggc gatacagtac acatttttgt
     5101 tgaaagaaaa aattacaaag gtgcatttat gcctggctat gtaaaatgga attcaactta
     5161 taaccctgca agtacaggac tattatatgt agatcattgt gtaggaaatg ttggttggaa
     5221 tcaaatgaac ccatgggtta agttttatga agatgtttta ggatttaaaa atattttaag
     5281 ttttgatgac aacgatattt ctacagaata ctcagcatta atgagtaaag taatgagcaa
     5341 tggaaacgga tttgtaaaat tccctatcaa cgaaccagca gaaggcaaga aaaaaagcca
     5401 agtagaagag tatttagatt tttatgatgg agaaggtgta caacacgtag ctattgcaac
     5461 aaataatatt attgaaacag ttacagattt acaaaatagg ggggtagaat ttttaaaaat
     5521 tccaccaagc tattatgaaa cagttttaga tagagttgga aaaattgatg aagacctaaa
     5581 accactaagt gaattaggca ttttaataga ccgagatgaa gaaggctact tattacaaat
     5641 tttttcaaaa ccattagaag ataggcctac tttatttttt gaaattatac aacgaaaagg
     5701 agctaaaagc tttggtaaag gaaattttaa agcattattt gaagccttag agagagagca
     5761 agatttaaga ggtaatttat aaattaagtt tctattaaaa ttaccatgaa caaaatattt
     5821 tggaagtatt ttattgcttt tggtttaata ataattttta aaaataccaa tgctcaaaag
     5881 atagattctt tacttaaaga agctgaaaag ttagaattta aattaaaaga ggttgatgct
     5941 ttaaataaat ataaagaagt attaactata tatggggata attttaaagc tctcataaaa
     6001 gcatctgaac
//
LOCUS       DAOQHP010000101         6009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7228_length_6009_cov_4.181391, whole genome shotgun sequence.
ACCESSION   DAOQHP010000101 DAOQHP010000000
VERSION     DAOQHP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_6009_cov_4.181391"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLY81_04515"
     CDS             complement(<1..439)
                     /locus_tag="PLY81_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF29519.1"
                     /translation="MKNFFLTLLFITSYSAIYAQPKKVVADKIIAQVGDKIILRSDVI
                     NAIEDYKRQGQAASLPPNPECAFLEAQLIQKALILQAEKDSLTVSDEEIEAALDNQIR
                     GFIQMYGSKEMLEEYSGRTIYQLKEDFRGPFRERKLADLMKSNI"
     gene            complement(662..1012)
                     /gene="rplS"
                     /locus_tag="PLY81_04520"
     CDS             complement(662..1012)
                     /gene="rplS"
                     /locus_tag="PLY81_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNF29520.1"
                     /translation="MNQAVQFVHEQLTATKNHPKFKAGDNVTVNYKIVEGGKERIQSF
                     RGDVIKIQGNGSTATFTVRKISDGVGVERLFPFASPNVDAIVLNRVGKVRRAKLFYLR
                     ERSGKSARIKEKRI"
     gene            complement(1110..1748)
                     /locus_tag="PLY81_04525"
     CDS             complement(1110..1748)
                     /locus_tag="PLY81_04525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNF29521.1"
                     /translation="MSKPFLDYLSDSELLIDKQYQPQTEKLLEYYELYKLVKNLNGSI
                     LKCGVNNNETFSYFSFFKKTNQYNTNQPLVVFEKNPSVFEPALVNNETVITVKSSEDI
                     ISSRKNIALKSKKEDIDFIPGNVTNALPTYLINNPELKIALLVIDLDNYEQTLAALQY
                     LYPRIVSNGILIISNYYKKAEDTAAIKEYFANQSVIIRNYSKSKGLHYIIKD"
     gene            1953..2660
                     /locus_tag="PLY81_04530"
     CDS             1953..2660
                     /locus_tag="PLY81_04530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNF29522.1"
                     /translation="MQLPFFYESNLTEQSNLFQLSSTSFKHAIQVLRLQKEDAIHITN
                     GKGLLCRAIILNADKKQATVKVEAIKFIEKEVVKTCIGISLLKNNNRFEWFLEKATEL
                     GITEIYPLLCSRTEKQHFRLERLNAIIISAMLQSQQAWLPTLLQPLSFNNIVVDKKDY
                     NIKLIAHCNLKEKVPLQKISIKENTMLLIGPEGDFTEDEIFLAEKNNFIAVSLGNNRF
                     RTETAGIAAAVLLKLNN"
     gene            2663..3838
                     /locus_tag="PLY81_04535"
     CDS             2663..3838
                     /locus_tag="PLY81_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29523.1"
                     /translation="MQIKEVNTKSLENDFITVNVLMNKSNKAYIRPLDNDINDIFNPN
                     KNKAYQNGNAKRWVLVDENGNLIGRIAAFISSKYINKNTNYLTGGIGFFDCINNQNAA
                     NTLFNTAKDWLQQNGAEAMDGPINFGDRDKWWGLMVEGFNDEPVFGMPFNPAYYQNLF
                     EQYGFKNYYNQYWYKMMVHDPLPARFKERYEKFAAKPDYYAKHADLKNLEKYAADFAK
                     VYNTAWAQHGEGKEVTKEQILKLFNTMKPIMDVRVIWFAYFKDEPIAMFINIPDINQY
                     IKYFNGKLNLLNKLKLLWMKKNRKHKRLTGLAFGVIPKFQALGVDSYIIQSCANLIQN
                     KNWYESYEMGWAGDWNPRMVNIYKSLGGSQNRRMVTYRYIFDESKYSFERHPVMEYN"
     gene            3885..4271
                     /gene="apaG"
                     /locus_tag="PLY81_04540"
     CDS             3885..4271
                     /gene="apaG"
                     /locus_tag="PLY81_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNF29524.1"
                     /translation="MISKISEGIKVNVECNYNTKHSKPILGDFIFSYCITIENYNAFT
                     VQLLKRKWHIFDSLGAYREVEGDGVVGVQPIIKPGDHFQYVSFCNLKTEIGKMQGSYE
                     LLNLNTLETFYVTIPEFELIAPLKYN"
     gene            complement(4268..5065)
                     /gene="lpxA"
                     /locus_tag="PLY81_04545"
     CDS             complement(4268..5065)
                     /gene="lpxA"
                     /locus_tag="PLY81_04545"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNF29525.1"
                     /translation="MTHPLTYIDPNAKIAANVKIDPFTVIHDNVKIGEGTWIGSNVTI
                     MEGTRIGKNCRIFPGAVIGAIPQDLKFNGEKTNVEIGDNTTIREFVTINRGTNDRGKT
                     KVGNNCLIMAYTHLAHDCIIGNNCILSNSVQMAGHVVVGDYAIIGGVSAVHQFVHIGQ
                     HTFIAGGSLVSKDVPPFMKAVRNPLSYGGVNSVGLKRRGFTLEQINHILDIYRIIYNK
                     GLNTSQAIDYIEQEIQASDERDDIIMFIRESGRGIIKRFTKGANDED"
     gene            complement(5128..>6009)
                     /gene="fabZ"
                     /locus_tag="PLY81_04550"
     CDS             complement(5128..>6009)
                     /gene="fabZ"
                     /locus_tag="PLY81_04550"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNF29526.1"
                     /translation="TQHAGLKTIKDFSQEIAPCRTFCFLHELEMLLDNDLIKGGDINN
                     AIVVVDKPISKEEMERLAVAFKRDKIEVKSEGYLNNLELRFPNEPARHKLLDIIGDLA
                     LIGYPIKARIIANRPGHSTNVEFAKRIKQYIKRNKHVKDVPVYDPLIPSVFTLEQIEK
                     TLPHRHPFLLVDKIVELTDTQIVGVKNVTFNEWFFPGHFPGNPVMPGVLQIEALAQTG
                     GILCINAMPKGEYNTYFLKIDNCKFKQMVKPGDTMLLKMELVSPIRRGICEMKGTVYV
                     AGKVATEAILVAQVIKK"
BASE COUNT         2081 a          892 c          879 g         2157 t
ORIGIN      
        1 caatattact cttcattaaa tcagctaatt ttctttctct aaagggccct ctaaaatctt
       61 cttttaattg ataaattgtt ctaccagaat attcttctaa catttcttta cttccataca
      121 tttgaataaa tccacgaatt tggttatcaa gtgctgcttc aatttcttca tcgctaactg
      181 ttagtgaatc tttttcggct tgcagtatta aagctttttg aattagttga gcttctaaaa
      241 aagcacattc ggggttggga ggtaaacttg cagcctgtcc ttgtctttta taatcttcaa
      301 ttgcattaat aacatcagaa cgtaaaatta ttttatctcc tacctgtgct attattttat
      361 cggctacaac cttttttggc tgggcataaa tggcactata agaagtaata aaaagtaggg
      421 ttaaaaaaaa atttttcatt tgtttatctt ttaatctttc ttgttatgaa agaatttatc
      481 ttacaataat ttgctattat gcttttttgt aacaaactat tttatggctc ttgtatttta
      541 tataaaagca atgttgtaaa aatagcagga gtttttaaaa atacagttta attataatgt
      601 tttaacaaaa taaaaaaagc ggaaattaaa tattccgctt ttaaaaataa gtatataata
      661 attagatacg tttttcttta attcttgcac ttttaccact tctttcacgt aagtaaaata
      721 attttgcacg gcgtacttta ccaactctat ttaaaacaat tgcatctaca tttggagagg
      781 caaacggaaa taaacgctca acaccaacgc catctgatat tttacgaact gtaaaagtag
      841 ctgttgaacc gttaccctga attttaatca catcgccacg gaaactttga atacgctctt
      901 taccaccttc tacaatttta tagtttacag ttacattatc gcctgcttta aacttaggat
      961 gattttttgt agctgttaat tgctcatgta caaattgaac tgcttgattc ataacacttt
     1021 ctttttttcg gattgcaaag gtaaggggtt gtaattaaat agaaaaaata aataggctgt
     1081 ttttgtaaat attattgagt aaaaagaaat taatctttaa taatatagtg taacccttta
     1141 ctttttgaat aattacgaat tataacactt tgatttgcaa agtattcttt aattgcagca
     1201 gtatcttctg cttttttata ataattacta attattaaaa taccgttgct tacaattctt
     1261 ggatataggt actgcaaagc agccaaagtt tgttcataat tgtctaaatc tatcactaat
     1321 aaggctattt taagctctgg gttattaata agataagtag gtaaagcgtt agttacattg
     1381 ccaggaataa aatcaatatc ttctttctta ctttttagtg caatattttt tctcgatgaa
     1441 attatatctt ctgaactttt tactgtaatt actgtttcat tatttactaa tgcaggttca
     1501 aatacagaag gatttttttc aaatacaacc aatggttgat tggtattata ttgattggtt
     1561 ttcttgaaaa aggaaaaata actaaaagtc tcgttattgt ttacaccaca ttttaaaata
     1621 gagccattaa ggttttttac taatttatac aattcgtaat actctaatag tttctctgtt
     1681 tggggttggt actgcttatc tataagcaat tcagagtctg acaagtaatc taaaaaaggc
     1741 tttgacatta aaatcaattg ttgattgttt aacaattggt tttttctata gagataattg
     1801 ggtacttaaa atgattaaat ttcgcttaaa taacggatta cttactatga tgaattttta
     1861 tatataaagg ttgcaaaaga gtataaaaaa tcatctgttt ttacttaaca tttctgatac
     1921 aactaaacca aactgtaaat aaatttgcca atatgcaatt accatttttt tatgagtcaa
     1981 atttaacaga acaaagcaac ttatttcaat taagttctac ttcttttaaa catgctattc
     2041 aagttttaag gctacaaaaa gaagatgcta tacatattac caatggcaaa ggtttattat
     2101 gcagggctat tattttaaat gcagataaaa aacaagctac ggttaaagta gaagctataa
     2161 agtttattga aaaagaggta gttaagacat gtattggtat ttcattatta aaaaataata
     2221 ataggtttga atggttttta gaaaaagcaa cagaattagg cattacagaa atttatcctt
     2281 tattatgctc cagaacagaa aaacaacatt ttaggttaga aagactaaat gctattatca
     2341 tttctgcaat gttacaaagc cagcaagctt ggcttcctac tttattgcag ccactaagtt
     2401 ttaataatat tgtagtggat aaaaaagatt ataatattaa gcttattgcc cattgtaacc
     2461 ttaaagagaa agtaccctta caaaaaattt caattaaaga aaatacaatg cttttaattg
     2521 gccccgaagg agattttaca gaagatgaaa tttttttagc cgaaaaaaat aattttatcg
     2581 ctgtatcatt aggtaataac aggtttagaa cagaaactgc aggtatagca gcagcagtat
     2641 tattaaaatt aaataattaa aaatgcaaat aaaagaagta aatacaaaat ctttagaaaa
     2701 tgattttatt actgtaaatg tgttaatgaa caaaagtaat aaggcatata ttaggccttt
     2761 agataatgat attaacgata tttttaaccc taacaaaaac aaagcatatc aaaacggcaa
     2821 tgctaaaaga tgggttttag tagatgaaaa tggaaattta attggaagaa ttgcagcttt
     2881 tatttcttca aaatatatca ataaaaatac caattactta acaggcggta taggtttttt
     2941 tgattgtata aataatcaaa atgctgccaa tactttattt aatacagcaa aagattggct
     3001 tcagcaaaat ggtgcagagg ctatggatgg accaattaat tttggagaca gagataaatg
     3061 gtgggggtta atggttgaag gttttaatga tgagcctgtt tttggaatgc cttttaaccc
     3121 tgcttactac caaaatttat ttgagcaata tggctttaaa aattattata accaatattg
     3181 gtataaaatg atggtacatg accctttacc agcaagattt aaagaaagat atgaaaaatt
     3241 tgctgctaag ccagactatt atgcaaaaca tgccgattta aagaatttag aaaaatatgc
     3301 tgccgatttt gcaaaagttt ataatactgc atgggcacaa catggcgaag gaaaagaagt
     3361 aaccaaagag caaattttaa agctttttaa tactatgaaa ccaattatgg atgttagggt
     3421 aatttggttt gcatatttta aagatgaacc aattgctatg tttataaaca taccagacat
     3481 taaccaatat attaagtact ttaatggtaa gttaaattta cttaataaac ttaaattgct
     3541 ttggatgaaa aaaaacagaa aacataaaag gcttacaggg cttgcatttg gtgtaattcc
     3601 taaatttcaa gcattagggg tggatagtta tatcatacaa tcttgtgcta atttaattca
     3661 aaacaaaaac tggtatgaaa gttatgaaat gggttgggca ggagattgga atcctagaat
     3721 ggtaaatatt tataaaagct taggtggtag ccaaaataga agaatggtta cctatcgtta
     3781 tatttttgat gaaagtaaat attcgtttga gaggcatcct gtaatggagt ataattaaca
     3841 agatttaaaa agttttaaac ttatctttac ttaaacaata atcaatgatt tcaaaaattt
     3901 ctgaaggcat aaaagttaat gtagagtgta attataacac taaacatagc aagcctattt
     3961 tgggagattt tatcttttct tattgtataa caattgaaaa ttataatgct tttaccgttc
     4021 aactactaaa aagaaaatgg catatttttg atagccttgg agcttacaga gaggtagaag
     4081 gtgatggtgt tgtgggtgta caacctatta ttaaaccagg agaccatttt caatatgtta
     4141 gcttttgcaa tttaaaaaca gaaattggca aaatgcaagg cagttacgag ttattaaatt
     4201 taaatacttt ggaaactttt tatgttacta ttccagaatt tgagttgatt gcaccattaa
     4261 aatataacta atcttcgtcg ttggcacctt tagtaaaacg ttttataatt cctctaccac
     4321 tttctctaat aaacattata atatcatctc tttcatcact agcttgaatt tcttgttcaa
     4381 tatagtcaat agcttgcgaa gtgtttaacc ctttattata tattattcta taaatatcta
     4441 aaatatgatt tatttgctct aaagtaaagc ctctgcgttt taagcctaca ctatttacac
     4501 caccatagct taatggattt ctaacagctt tcataaaagg aggtacgtct ttactaacta
     4561 aactaccacc tgcaataaaa gtatgttggc caatgtgcac aaattgatgt acagcactta
     4621 cacctccaat aatagcataa tctcctacta caacatgccc agccatttgt acactattac
     4681 ttaaaataca attattgcca ataatacaat catgtgctaa atgggtgtaa gccataatta
     4741 agcagttatt gccaactttt gttttacctc tatcgttggt gcctctattg atagtaacaa
     4801 actctctaat tgtagtgtta tcccctattt ctacattcgt tttttcgcca ttaaatttta
     4861 aatcttgtgg tattgcacca attactgcac ccggaaaaat tctacaattt ttacctattc
     4921 ttgttccttc cattattgta acattgctac caatccaggt tccttctcca atttttacat
     4981 tatcatgtat aaccgtaaaa gggtctattt ttacgttggc tgcaatttta gcatttgggt
     5041 caatatatgt taatggatgg gtcatacaaa tttatttatt tgaaagagaa tagggtttat
     5101 gaaaaatatt tatcaaaatt agtttcctta ttttttaatt acttgggcta ctaaaattgc
     5161 ttctgttgca acttttcctg ctacataaac agtacctttc atttcgcaaa tgcctcttct
     5221 tataggcgat actaattcca tttttaaaag catagtatcc cctggtttta ccatttgttt
     5281 aaatttacaa ttatctattt ttaagaaata agtattgtat tcacctttag gcattgcatt
     5341 aatacacaaa atgccaccag tttgtgctaa agcttcaatt tgtaaaactc ctggcattac
     5401 aggatttcct ggaaaatgac ctgggaaaaa ccattcgtta aaagttacat ttttaactcc
     5461 tacaatttgg gtatctgtta gctccacaat tttatcaacc aataagaaag gatgacgatg
     5521 tggtaatgtt ttttcaattt gttctaaagt gaaaacagaa ggaattaaag gatcataaac
     5581 aggtacatct ttaacgtgtt tatttctttt tatatattgc tttattcttt tggcaaactc
     5641 cacattagtg ctatgtcctg gcctattggc tataattctg gctttaatag gatagccaat
     5701 taatgcaaga tctccaataa tatctaaaag cttatgcctt gcaggctcat taggaaaacg
     5761 aagctctaaa ttgtttaagt atccttctga ttttacttct attttatctc ttttaaatgc
     5821 tactgcaagc ctttccatct cttcttttga aattggctta tctacaacca caatggcatt
     5881 attaatatcg ccacctttta ttaaatcatt atctaatagc atttctaatt catgcaaaaa
     5941 acaaaatgtt ctacaaggtg caatttcctg gctaaaatct ttaattgttt ttaatccggc
     6001 atgctgtgt
//
LOCUS       DAOQHP010000102         5928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7404_length_5928_cov_3.191214, whole genome shotgun sequence.
ACCESSION   DAOQHP010000102 DAOQHP010000000
VERSION     DAOQHP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7404_length_5928_cov_3.191214"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..394
                     /gene="rpmB"
                     /locus_tag="PLY81_04555"
     CDS             158..394
                     /gene="rpmB"
                     /locus_tag="PLY81_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNF29527.1"
                     /translation="MARVCQITGKKPLTGNSVSHSNIKTKRRFLPNLQTKRFFFTEEN
                     RWITLKLTTEGIRTINKNGLATVIKDLRAQGEKI"
     gene            432..614
                     /gene="rpmG"
                     /locus_tag="PLY81_04560"
     CDS             432..614
                     /gene="rpmG"
                     /locus_tag="PLY81_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNF29528.1"
                     /translation="MAKKGNRVQVILECTEHKTSGKPGTSRYITVKNKKNTPERLELK
                     KFNPILKKVTLHKEIK"
     gene            617..790
                     /locus_tag="PLY81_04565"
     CDS             617..790
                     /locus_tag="PLY81_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HNF29529.1"
                     /translation="MAKAASKNSKVKDLKASAEAKNWTKVIKSVRSPKTGAYTFKEAI
                     VHKDKVKDYLASK"
     gene            942..1904
                     /gene="ftsY"
                     /locus_tag="PLY81_04570"
     CDS             942..1904
                     /gene="ftsY"
                     /locus_tag="PLY81_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNF29530.1"
                     /translation="MGFFNKLFGKKEKETLNDGLQKTKEGFFTKLTKAIAGKSTVDAE
                     VLDNLEEALIGADVGVETTLQIIEGIEKRVKEEKYISTAELNKVLQNEIQKMLTDAND
                     DAYRNFEIPTNKKPYIILVVGVNGVGKTTTIGKLAHNYKKAGYNVLLGAADTFRAAAV
                     DQLTIWSERVGVPIVKQEMGSDPSAVAFDTVQSAIAKGSDVVLIDTAGRLHNKTHLME
                     ELSKIKRVIKKNISDAPHEVMLVLDGSTGQNALEQAKQFTAATDVNALTITKLDGTAK
                     GGVVLAIANQFKIPVKFIGVGEKIDDLIIFDKQEFVDSLFKLEN"
     gene            1939..3249
                     /gene="rimO"
                     /locus_tag="PLY81_04575"
     CDS             1939..3249
                     /gene="rimO"
                     /locus_tag="PLY81_04575"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNF29531.1"
                     /translation="MKAKSLKQDKVNIITLGCSKNLVDSEVLSGQLLANEIDVVHENK
                     KLDHNIVVINTCGFIDKAKEESINTILDQLELKRRGKLDKVYVTGCLSERYREDLAKE
                     IAEVDAWFGTLELPLILKQFNADYKTELLGERLLSTPKHYAFMKISEGCNRTCSFCAI
                     PLMRGNHISKPIEVLVKEAETLVQKGVKEVMLIAQELTYYGLDIYKKRSLPKLLNALA
                     DVKGLEWIRLHYAYPSKFPLEIVEVMKERENICNYLDMPLQHASDAMLKAMRRNITRE
                     EMNELIYKIRNIIPDICLRTTLISGFPGETEKDVEELKDFLKIHRFDRVGIFTYSHEE
                     NTGAYTLEDTIPQTEKEARAQEIMEVQQEISLEKNQAKVGKTFKVIIDKKEAGRYLGR
                     TEFDSVEVDNEVVIQSDKKLTIGSFVNVKITKAYDYDIEGVVVE"
     gene            complement(3269..3778)
                     /locus_tag="PLY81_04580"
     CDS             complement(3269..3778)
                     /locus_tag="PLY81_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF29532.1"
                     /translation="MSLNFETNLTNIQQLASNKEKENDDFISYLKSFDGEYIDKQVHL
                     LNEIVSPKIDCTLCGNCCKTLLINVQEIEANNLAAHLEINRAIFDEQFIEKGSNGMML
                     IKTVPCSFLKDNKCTVYNFRFEGCKEFPAMHLPNFKDRLFTTFMHYNRCPIIYNIIEQ
                     LKLVLNFKE"
     gene            3933..5822
                     /locus_tag="PLY81_04585"
     CDS             3933..5822
                     /locus_tag="PLY81_04585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNF29533.1"
                     /translation="MSEIKKMTNYTEDDIKSLDWKEHIRLRPGMYIGKLGDGSSADDG
                     IYVLLKEVLDNCVDEHTMGFGKHIDISIENKTVTIRDFGRGIPLGKVVDVVSKINTGA
                     KYDSKAFQKSVGLNGVGTKAVNALSNYFKVESFRDGKTKVAEFERGVLLKEHKEAATT
                     EENGTMVTFVPDESIFHNYKFLNEYLDNQFWNYCYLNAGLVINFNNKKYVSKNGLLDL
                     LNRKTNAEELRYPIIHLKGEDIEVAISHNNDYGEDIYSFVNGQYTTQGGTHYQAFREA
                     YVKTIRDFFKKDYDTADIRQSIVAAVSVRVQEPVFESQTKTKLGSSVVYEGGPSMKKF
                     IEDFLTKELDNFLHRNTSVADALRRRIEQSEKERKELSGIRKLANERAKKANLHNKKL
                     RDCRVHLNDEPPNKNKEEFIELQNKSTIFITEGDSASGSITKARSVDTQAVFSLRGKP
                     LNSYGLSKKIVYENEEFNLLQHALNIEDGLENLRYKNVVIATDADVDGMHIRLLLMTF
                     FLQFFPDLVKQNHVYILETPLFRVRNKQETIYCYDETEKQNAIHKLGGKPEITRFKGL
                     GEISPVEFERFINEDIRLQPVLLEKGEHIQQLLEYYMGKNTPQRQEFIIDNLKVELDI
                     VETVV"
BASE COUNT         2361 a          740 c         1021 g         1806 t
ORIGIN      
        1 attggtttta tctataaaaa tgctgtacaa aatggcataa ttttgttagt attgcattac
       61 tttttaagac aaaaagcgtt atttgaatat ttttttaaat taactattgt tatttccata
      121 aaacccccta cttttgccgt cccgaaaaaa tagagttatg gctagagtat gtcaaattac
      181 agggaagaaa cctcttacag gaaatagtgt ttctcactca aacattaaaa ctaagcgtag
      241 gtttttacct aatttacaaa ccaaacgttt cttttttact gaagaaaacc gttggataac
      301 attaaaactt acaacagaag gtataagaac catcaacaaa aatggcttag ctactgttat
      361 taaagattta agagcacaag gagaaaaaat ttaaacctaa aaggttttaa tagtatataa
      421 attttattaa aatggcaaag aaaggcaaca gggtacaagt tatattggag tgtacagagc
      481 ataaaacttc tggaaaacca ggcacaagcc gctatataac tgtgaaaaat aaaaaaaata
      541 cccctgaaag attagagtta aaaaaattta accctatttt aaagaaagta acattacata
      601 aagaaattaa ataatcatgg caaaagcagc ttcaaaaaac tcaaaggtaa aagaccttaa
      661 agcttcggca gaagctaaaa actggactaa agtaattaaa tctgtacgta gtcctaaaac
      721 aggtgcatac acttttaaag aagccattgt gcacaaagac aaagtaaaag actatttagc
      781 ttctaaataa ttttttatca ataatagtat ttctaataaa ggctttctgt aaagtacaga
      841 aggctttttt agtttcttat ttaactaaat ttgcttactg ataaaaagta gcgaaatatt
      901 caaaacacaa ttctttttta catttcagaa tcttcataat tatgggcttt tttaataaac
      961 tttttggcaa aaaagaaaaa gaaactttaa acgatggttt gcaaaaaact aaagaaggtt
     1021 tttttacaaa attaacgaaa gcaattgcag gcaaaagtac tgttgatgca gaagttttag
     1081 ataatttaga agaggcatta ataggtgctg atgtaggcgt tgaaacaact ttgcaaatta
     1141 ttgagggtat tgaaaaaaga gtgaaagaag aaaaatatat aagtacagca gaattgaata
     1201 aagtattgca gaatgaaatt caaaaaatgc ttaccgatgc taatgatgat gcttatagaa
     1261 acttcgaaat tcctaccaat aaaaaaccat atatcatttt agttgttggg gttaatggtg
     1321 ttggtaaaac taccacaatt ggtaaattgg cacataatta taagaaagca ggatacaatg
     1381 tattattagg tgcagccgat acttttagag ctgcagctgt agatcaactt actatatgga
     1441 gtgaacgtgt tggagtgcca attgtaaaac aagaaatggg aagtgaccca agtgctgtag
     1501 catttgatac agtacaaagt gccattgcaa aaggtagcga tgttgtttta attgataccg
     1561 caggaaggct gcataataaa acacatttaa tggaagagtt aagcaaaata aaaagagtaa
     1621 taaagaaaaa tatttcagat gcaccacacg aagtaatgct ggttttagat ggttctacag
     1681 gtcaaaatgc tttagaacaa gcaaagcaat ttactgcagc taccgatgtg aatgctctta
     1741 ctataactaa attagatggt actgcaaaag gaggcgtagt acttgctatt gccaaccaat
     1801 ttaaaatacc tgttaagttt ataggtgtag gcgaaaaaat agacgactta atcatttttg
     1861 ataaacaaga atttgtggat agtttattta aacttgaaaa ctaaaaaaat acctttcaca
     1921 catctttaag aaattttaat gaaagcaaaa tcgctaaaac aagataaagt aaatattata
     1981 acattaggtt gtagtaaaaa cctggttgat agtgaagttt taagcggcca gcttttagcc
     2041 aatgaaatag atgtagtaca cgaaaataaa aaactagatc ataacattgt agtaattaat
     2101 acctgtggtt ttattgataa agctaaggaa gaaagtatca atacaatttt agaccagctt
     2161 gaattaaaac gaagaggtaa attagataaa gtgtatgtta caggctgctt aagcgaaaga
     2221 tacagagaag accttgctaa agaaattgct gaggtagatg catggtttgg tacattagaa
     2281 ttgccattaa ttttaaaaca atttaatgcc gattataaaa ctgaattatt gggcgaaagg
     2341 cttttaagta caccaaaaca ttatgccttc atgaaaatta gcgaaggctg taacagaact
     2401 tgtagctttt gtgcaatacc cttaatgcgt ggcaaccata ttagtaaacc aattgaagtt
     2461 ttagttaaag aagctgaaac acttgtacag aaaggggtta aagaagtaat gttaattgct
     2521 caagagctta cttactatgg tttagatatt tataaaaaac gttcgttgcc aaaactatta
     2581 aatgcattag cagatgttaa aggattagag tggattagac ttcattacgc atatccttcc
     2641 aaatttcctc ttgaaatagt tgaggtaatg aaagaaagag aaaatatttg taattattta
     2701 gatatgcctt tgcaacatgc aagtgatgca atgttgaagg caatgagaag aaatattaca
     2761 agagaggaaa tgaatgaatt aatctataaa ataagaaaca ttatcccaga tatttgctta
     2821 cgtacaacat taatttcagg atttcctggc gaaacagaaa aggatgtaga agagttaaaa
     2881 gattttttaa aaattcaccg ctttgacagg gtaggaatat ttacttatag ccatgaagaa
     2941 aatacaggag cttatacttt agaagacaca attccgcaaa cagaaaaaga agctagagca
     3001 caagaaataa tggaagtgca acaagaaata agtttagaaa aaaatcaagc taaagttggt
     3061 aaaacattta aagttattat tgataaaaaa gaagcaggaa gatatttagg tagaactgaa
     3121 tttgatagtg tagaagtaga taatgaagta gtaatacaat cagataagaa acttacaata
     3181 ggaagttttg taaatgtaaa aattactaaa gcctacgatt atgatataga aggagtagtg
     3241 gttgaataag cataaataat gtaaaaaatt actctttgaa atttaaaacc aactttaatt
     3301 gttctatgat attgtaaata attggacacc gattatagtg cataaaagta gtaaaaagcc
     3361 tatccttaaa attaggcaaa tgcatagcag gaaattcttt acatccttca aacctaaaat
     3421 tataaacagt acatttatta tcttttaaaa aactacatgg tacagtttta attaacatca
     3481 taccattaga gcctttttct ataaattgtt catcaaatat tgctctgtta atttctaaat
     3541 gtgcagctaa attatttgct tcaatttctt gcacgtttat taaaagtgtt ttgcaacaat
     3601 ttccgcacaa agtgcaatca atttttggag atactatttc atttaaaaga tgtacttgtt
     3661 tatcaatata ttctccatca aaactcttta aataagaaat aaaatcatcg ttttcttttt
     3721 ctttattact agctaattgc tgaatatttg taagatttgt ttcaaaattt aaagacatat
     3781 aaacaaactt atgttattta tttcatttgc ctgcaaatcc ttgttttatc cacatttcat
     3841 tggcttgtgt ttataatcta tactttagat gccttaatgc tttgttagat ttgtaactaa
     3901 gaaaactata aaaaatagca acaatttttc aaatgagtga aattaaaaaa atgacaaatt
     3961 ataccgaaga tgatattaaa agtttagatt ggaaagagca tataagatta aggcctggta
     4021 tgtacattgg taaattagga gatggctcat ctgctgatga cggtatttat gttttattaa
     4081 aagaagtttt agataattgt gttgatgaac atacaatggg ttttggaaag catattgata
     4141 tttctataga aaataaaact gtaaccatca gagattttgg tagaggcatt ccattaggca
     4201 aggttgtaga tgttgttagt aaaataaata caggagcaaa gtacgatagt aaagcatttc
     4261 aaaaaagtgt tggtttaaat ggcgttggta caaaggctgt aaatgcgtta agcaattatt
     4321 ttaaggtaga aagttttaga gatggaaaaa caaaagtggc agagtttgaa agaggtgtat
     4381 tattaaaaga acacaaagaa gcagctacaa cagaagaaaa tggaacaatg gtaacatttg
     4441 ttccagacga atctattttt cataattata aattcttaaa tgaatattta gacaatcaat
     4501 tctggaatta ttgttactta aatgcagggt tggttattaa ttttaacaac aaaaaatacg
     4561 tcagcaaaaa tggcttacta gatttactaa acagaaaaac caatgcagaa gaattacgat
     4621 atcctattat tcatttaaaa ggagaagaca tagaagttgc aattagccat aataatgatt
     4681 atggagaaga tatttattct ttcgtgaacg gtcaatatac aacacaaggt ggtacacact
     4741 atcaggcttt tagagaagct tatgtaaaaa caatcagaga cttttttaaa aaagattatg
     4801 atactgcaga tattcgccaa agcatagttg cagcagttag tgttagagta caagagccag
     4861 tttttgaaag tcaaacaaaa acaaaattag gatcctctgt ggtgtatgaa ggtggcccaa
     4921 gcatgaaaaa atttatagaa gattttttaa ccaaagaatt agataatttt ttacatagaa
     4981 atacatctgt agcagatgct ttaagaagaa ggatagaaca aagcgaaaaa gaaagaaaag
     5041 aattaagtgg aattagaaaa ttagcaaatg aacgagccaa aaaagctaac cttcataata
     5101 aaaaacttag agattgtaga gtacatttaa acgatgagcc acctaacaaa aacaaagaag
     5161 agtttataga gttacaaaat aaaagtacca tttttataac agaaggagat agtgcaagtg
     5221 gtagtataac aaaagctaga agtgtagata cacaagcagt ttttagcctt agaggtaaac
     5281 ctttaaatag ctatggttta agcaaaaaaa ttgtttacga aaacgaagag tttaacttat
     5341 tgcaacatgc attaaatatt gaagatggct tagaaaacct tagatataaa aatgtggtaa
     5401 ttgctaccga tgctgatgtt gatggaatgc acattcgttt acttttaatg actttctttc
     5461 tgcaattttt ccccgatctt gttaaacaaa accatgttta tattttagaa acccctttat
     5521 ttagagttcg taataaacaa gaaactattt attgttatga cgaaactgaa aaacaaaatg
     5581 caattcataa acttggagga aagcctgaaa taacaaggtt taaaggctta ggtgaaatta
     5641 gcccagttga atttgaaaga tttataaatg aagatattcg gttacagcca gtattgttag
     5701 aaaaaggaga acatattcaa caattgttag aatattatat gggaaaaaat acaccacaaa
     5761 ggcaagagtt tattattgat aacttaaaag tagaattaga tattgttgaa acagtggttt
     5821 aaactttatt attttaattt ttatataaat gttcgaattt cataaagata gaaaaaggta
     5881 ttttgatatt caggttgcta atgccagtga atatgttatt ccttttat
//
LOCUS       DAOQHP010000103         5923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7415_length_5923_cov_3.297716, whole genome shotgun sequence.
ACCESSION   DAOQHP010000103 DAOQHP010000000
VERSION     DAOQHP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7415_length_5923_cov_3.297716"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLY81_04590"
     CDS             complement(<1..891)
                     /locus_tag="PLY81_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF29534.1"
                     /translation="MSETLFKVTIDNIVIEVPAGTTILNAARKIGGEVAPPTMCYYSK
                     LEGSGGKCRTCLVEVSKGSEKDPRPMPKLVASCRTTVMDGMEVKNITSERVLTARAGV
                     VEFLLINHPLDCPICDQAGECYLQDLSYEHGADKSRFEFQKRTFKKVDLGDKIQLHMT
                     RCILCYRCVLVANQLTNQREHGILDRGDHAQIATYIQKNLQNDFIGNIIDVCPVGALT
                     DKTFRFKNRVWFTKPVDAHRDCNNPKCCGEVRLWMRGDEVYRVTGRIDEWGEVKDASN
                     GKPGWICNECRFDKKKVSDWV"
     gene            complement(1007..2362)
                     /gene="nuoF"
                     /locus_tag="PLY81_04595"
     CDS             complement(1007..2362)
                     /gene="nuoF"
                     /locus_tag="PLY81_04595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNF29535.1"
                     /translation="MGSIKLLLEKAHVEGIRYYDVYRREGGYRSVEKALKTMSPETIV
                     EEVKKSGLRGRGGAGFPAGMKWSFIAKPEGVPRHLVCNADESEPGTFKDRYLMEFIPH
                     LLIEGLIVSSFALGSNDTYIYIRGEYAWIVDILDHAILEVKNNGWLGKNILGSGFDCN
                     IHVQRGAGAYICGEETALIESLEGKRGNPRIKPPFPAVQGLWMRPTVVNNVETLAAVV
                     PIINMGGEEYAKIGVGRSTGTKLISACGNINKPGVYEIDMTISVEEFIYSDEYCGGIA
                     NGKRLKACIPGGSSVPILPANLLLKTAKGETRFMNYESLSDGGFASGSMMGSGGFIVL
                     DEDQCVVKNTYTLARFYRHESCGQCSPCREGTGWMEKILWNIESGKGKMSDIDLLWDV
                     QSKIEGNTICPLGDAAAWPVAAAIRHFRDEFEWHITNKEEAQKRNFGLAHYANPIHVP
                     A"
     gene            complement(2479..2979)
                     /locus_tag="PLY81_04600"
     CDS             complement(2479..2979)
                     /locus_tag="PLY81_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNF29536.1"
                     /translation="MIQFNDTQMAEFKRLVNRYPADKKKSALLPVLHLAQDSFGGWLS
                     TETMDYVAALLEIKPIEVYEVATFYSMYNLKPVGKYMFEVCQTGPCMLRGSDDIIAYI
                     NEKLGIKPGETTADGMFTLKTVECLGACGYAPMMQLGKHYKEHLTKQKIDEIIEDCKK
                     AAINNN"
     gene            complement(3051..4283)
                     /locus_tag="PLY81_04605"
     CDS             complement(3051..4283)
                     /locus_tag="PLY81_04605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF29537.1"
                     /translation="MSDILHHNIKLPKGAIEKETTTLNLGPTHPATHGVMQNIIELDG
                     ERIVSAEQTIGYIHRAFEKLAERRPLFQITTLTDRLNYCSAPLNNMGWHLTCEKLLGI
                     QTPKRVDYLRVIIMELSRITDHLICNSIVGVDSGAFTGFLYVMQYRELVYEIYEEICG
                     ARLTTNMGRIGGFERNFNDTAWKKLDKFLVEFPKAWKEFGNLFLRNRIFMDRLIGTGA
                     ISAERALNYGFTGPNLRAAGVDYDVRVHTPYSSYQDFNFTIPVGTTGDNYDRFLVRDQ
                     EVWQSISIIQQAYQKVQEFKGAEAEVYHADAPEYYLPEKELVYTRMESLIWHFKIVMG
                     ETEIPKGEVYNSIEGANGELGFYLISDGGRTPYRLHFRRPCFIYYQAYSELTKGGMLS
                     DAIMTMSSLNLIAGELDA"
     gene            complement(4325..4846)
                     /locus_tag="PLY81_04610"
     CDS             complement(4325..4846)
                     /locus_tag="PLY81_04610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNF29538.1"
                     /translation="MMALNNEIVKQKLVEKFGEQLTNWEEPYNFLTFVAPKDLNLKVL
                     QFLYDDETLKFRFLTDLCAVHYPDRKGEEVAVVYHLHNLENNIRVRFKVYTDAEKTEV
                     YTATALYEAANWMERETYDFFGVNFIGHPNLKRILNVDEMDYFPLRKQYPLEDQTRVD
                     KDDEMFGRGGSIL"
     gene            complement(4870..5457)
                     /locus_tag="PLY81_04615"
     CDS             complement(4870..5457)
                     /locus_tag="PLY81_04615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNF29539.1"
                     /translation="MRPVKYNIHPKEAKLADSLIVSEMPDGYTGEGFFATKLSDVVGL
                     ARKNSIWPMPFATSCCGIEFMATMASHYDLARFGSERVGFSPRQCDLLMVMGTIAKKM
                     GPVLRQVYIQMAEPRWVMAVGACASTGGIFDTYSVLQGIDQIIPVDVYVPGCAPRPEA
                     ILDGVMRIQDIVQNESIRRRNTDHYKALLNSYGIE"
     gene            complement(5524..5919)
                     /locus_tag="PLY81_04620"
     CDS             complement(5524..5919)
                     /locus_tag="PLY81_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNF29540.1"
                     /translation="MHHFLLNASNANTTSNYLPIGIQLIFAVGFVATMLFLSAMVGPK
                     RRTKEKLETFTSGIESHGDARQPVSVKYFLVAILFVLFDVEVIFFYPYAVNFKMLGWE
                     GFLAVLMFVALFLVGFIYIIKKKALKWED"
BASE COUNT         1759 a         1227 c          788 g         2149 t
ORIGIN      
        1 tacccaatcg cttactttct ttttatcaaa tctacattcg ttgcatatcc aacctggttt
       61 gccattactt gcatctttca cttcacccca ttcatcaatt cttcctgtta ctctatatac
      121 ttcgtcacct ctcatccata atctaacttc tccgcagcat ttagggttat tgcaatctct
      181 atgagcatcc acaggtttag taaaccatac acggttttta aatctaaaag ttttatctgt
      241 taatgcacct accgggcata catcaattat gtttcctata aaatcgtttt gtaaattttt
      301 ctgaatataa gtggcaattt gtgcatggtc gcctctgtct aaaatgccat gttctctttg
      361 gttagttagt tgattggcta ctaacacaca acgatagcat aaaatacaac gtgtcatgtg
      421 tagttgtatt ttatctccta aatctacttt tttaaaggtt cttttttgaa attcaaatct
      481 agatttatct gctccatgtt cataacttaa atcttgtaaa taacattcgc ctgcttggtc
      541 acaaattggg caatctaagg ggtggtttat taataaaaat tcaactacac ctgctcttgc
      601 agttaatact ctttcggatg taatgttttt tacttccatt ccatccatta cagtggtacg
      661 gcagcttgcc actaatttag gcatagggcg tgggtctttt tcggagcctt tacttacctc
      721 tactaaacaa gttctgcatt tacctccact gccttctaat ttgctatagt aacacatagt
      781 aggtggagca acttctcccc caatttttct tgcagcattt aatattgtag tacctgctgg
      841 tacttcaata acaatgttat caatggttac tttaaataat gtttcactca taatattaat
      901 tcaaactaaa catctaataa tacaattctc acttttaacc tatttcttca ataatacttc
      961 tgtttaattt taaagttatt ttcttacata tttctctact caaaaattaa gcaggcacat
     1021 gaattggatt ggcataatgt gctaatccaa aatttctttt ttgtgcttcc tctttattgg
     1081 taatatgcca ttcaaactca tctctaaaat gcctaatggc tgctgcaact ggccaagctg
     1141 ctgcatcgcc taatgggcaa attgtatttc cttcaatttt agattgcaca tcccacaata
     1201 aatctatatc actcattttc ccttttccac tttcaatatt ccataatatt ttttccatcc
     1261 agccagtgcc ttctctacaa ggcgagcatt gcccacaact ttcatgacga taaaatcttg
     1321 ccagggtgta ggtatttttt actacacatt gatcttcatc taaaacaata aagcctccgc
     1381 tacccatcat acttcctgaa gcaaaacctc catcgctaag gctttcataa ttcataaatc
     1441 ttgtttcgcc ttttgctgtt ttaagtaaga ggttggcagg taaaataggc actgacgacc
     1501 cgcctggaat acaggctttt agtcttttac catttgctat tcctccacaa tattcatcac
     1561 tataaataaa ttcttctacg cttatggtca tatcaatttc atacacccct ggtttattaa
     1621 tatttccaca agccgatatt agttttgttc ctgtacttct gcctacacca atttttgcat
     1681 attcttctcc acccatatta atgattggta caacagcagc taaagtttct acattattta
     1741 ctacagtagg tctcatccat aaaccttgta ctgcaggaaa aggaggtttt attcttggat
     1801 ttcctctttt accttcaagg ctctcaatca atgcagtttc ctctccacat atataagctc
     1861 ctgcaccacg ttgtacatga atattacaat caaagcctga gcctaaaata tttttaccta
     1921 accagccatt atttttcact tctaaaatgg catgatctaa aatatctaca atccaagcat
     1981 attctccacg tatataaata tatgtatcgt tgctgcctaa tgcaaacgaa gaaactatta
     2041 atccttctat tagcaaatgg ggtataaact ccattaaata tctgtcttta aacgtacctg
     2101 gttcactttc gtctgcatta caaactaaat gacgaggcac accttctggt tttgcaataa
     2161 agctccattt cattccagca ggaaaaccag caccaccacg accacgtaac ccacttttct
     2221 tcacttcttc aacaatagtt tcggggctca ttgtttttaa agctttttct acgcttctat
     2281 aaccaccttc acgcctgtac acatcataat acctgatgcc ttctacatgt gctttttcta
     2341 ataataattt tatactgccc atattgtatc tctaaagaga tttttaaaat ttattttttt
     2401 gtactctgct tttctgcaac tatttagctt ttgttgcgtc gcacacttgt actgtaacaa
     2461 gcatagccac cattagtttt aattattgtt tattgctgct tttttacaat cttcaataat
     2521 ttcatctatt ttttgtttgg ttaaatgctc tttataatgc tttcctaact gcatcatagg
     2581 ggcataacca caggctccta aacattctac tgtttttaat gtaaacattc catctgccgt
     2641 tgtttcgcca ggttttattc ctaatttttc atttatatat gcaataatat catcgctgcc
     2701 tcttaacata caaggaccag tttggcaaac ttcaaacata tatttaccaa caggttttaa
     2761 attgtacatg ctataaaaag tagctacttc atatacttct attggtttaa tttctaaaag
     2821 ggcagctaca taatccatag tttcggtgct taaccaacca ccaaagctat cttgtgccaa
     2881 atgcagtaca ggcaataagg cactcttttt tttatcagca ggatagcggt ttactaacct
     2941 tttaaattct gccatttgtg tatcgttaaa ctgtatcact tttctatttt aatttttaat
     3001 tttaaataac tgaaataatc aatttttaaa attttacttt ctatatttta ttaagcatct
     3061 agctcaccag ctattaaatt taagctactc attgtcataa ttgcatcgct caacatgcca
     3121 ccttttgtta actcgctata cgcctgataa taaataaagc aagggcgtct aaaatgtaat
     3181 ctataaggtg ttctgcctcc atcgctaatt aaataaaagc ccaattctcc gtttgctcct
     3241 tcaatgctat tatatacttc ccctttgggt atttctgttt cgcccattac aattttaaaa
     3301 tgccatatta aagattccat tctggtataa accagttctt tttcaggcaa ataatattct
     3361 ggtgcatctg catggtacac ttctgcttcg gctcctttaa attcttgtac tttttgatat
     3421 gcttgttgaa tgattgaaat ggattgccaa acttcttggt ctctcactaa aaatctatca
     3481 taattatcgc cagttgtacc tactggaata gtaaaattaa aatcttggta actactataa
     3541 ggtgtatgta ctctaacatc ataatctaca ccagcagctc ttaaatttgg accagtaaag
     3601 ccatagttta aagctctttc ggcactaatt gcaccagttc caattaaacg atccatgaaa
     3661 attctgtttc ttaaaaacag attgccaaat tctttccaag ctttaggaaa ttcaactaaa
     3721 aatttatcta atttcttcca tgcggtatca ttaaagtttc tttcaaaacc gccaatacgc
     3781 cccatgtttg ttgttaatct tgcaccacat atttcttcat aaatttcata caccaattct
     3841 ctgtattgca ttacgtataa gaatcctgta aaagcaccac tatctacccc aacaatagaa
     3901 ttgcaaatta agtggtcggt aatacgagat aattccataa taattactcg taaataatct
     3961 acccttttag gtgtttgaat gcctaataat ttttcgcaag tgaggtgcca acccatattg
     4021 tttaaagggg ctgaacaata atttaatcta tctgttaatg tggtaatttg aaataggggt
     4081 ctgcgttctg ctaatttttc aaaagctcta tgtatatagc caatagtttg ctctgcactt
     4141 acaatacgtt ctccatctaa ctcaataata ttttgcatta caccatgtgt tgcagggtgt
     4201 gttgggccaa ggtttaatgt agttgtttct ttttctattg caccttttgg cagttttata
     4261 ttatggtgta aaatatctga cataattttt tttaaagctg attataattt ttagttagat
     4321 gaaattacaa aatagagcct ccacgaccga acatttcatc atctttatca actcttgttt
     4381 gatcttctaa tggatattgt tttcttaacg gaaaataatc catttcatct acatttaata
     4441 ttctttttaa attaggatga ccaataaagt ttactccaaa aaaatcgtat gtttctcttt
     4501 ccatccaatt ggcagcttcg tatagtgctg ttgctgtata tacttctgtt ttttctgcat
     4561 cggtatatac tttaaatctt actctaatat tgttttctag attgtgcaaa tgataaacaa
     4621 ctgcaacttc ttcgcctttt ctatctggat aatgaactgc acacaaatcg gttaaaaatc
     4681 taaatttaag agtttcatca tcatataaaa actgaagtac ttttaagttt aaatctttgg
     4741 gggcaacaaa agttaggaag ttgtaaggtt cttcccaatt ggttaattgt tctccaaatt
     4801 tttcaactaa tttttgtttt actatttcgt tgtttaatgc catcatttaa aaattcaact
     4861 taataaattt tattcaattc cgtaactgtt taataaagcc ttatagtggt ctgtgtttct
     4921 tcttctaata ctttcatttt gtactatatc ttgtattctc atcactccat ctaaaatagc
     4981 ttctggtctt ggtgcacagc caggcacata cacatctaca ggtattatct ggtctattcc
     5041 ttgtaataca ctataggtat caaaaatacc accagtgctt gcacaagctc caacagccat
     5101 tacccaacgt ggttcagcca tttgtatata aacctgacgt agtacaggcc ccattttttt
     5161 agctattgtt cccataacca ttaacaaatc gcactggcgt ggagaaaagc ccacacgttc
     5221 gcttccaaag cgtgctaaat cgtaatggct tgccatggta gccataaatt ctataccaca
     5281 acaactggtt gcaaaaggca ttggccaaat agaatttttg cgtgccagac caactacatc
     5341 gctaagcttt gttgcaaaaa atccttcgcc agtatatcca tcaggcattt cgctaacaat
     5401 taaactgtcg gctaattttg cttcttttgg atgtatatta tattttactg gtctcatttt
     5461 aaatttataa tttttacttc tggttactaa ccatattctt acataatgct tacaattgtt
     5521 tatttaatct tcccacttta atgctttttt cttaattata taaataaatc caactaagaa
     5581 aagagctaca aacattagaa cagctaaaaa gccttcccag cccaacattt taaaattaac
     5641 tgcataaggg taaaagaaga taacctctac atcaaacaac acaaataaaa tagccactaa
     5701 aaaatattta accgaaactg gttgccttgc atcgccatga gattctatgc cactagtaaa
     5761 tgtttctagt ttttctttgg ttcttctttt tgggccaacc attgcagata aaaataacat
     5821 agttgcaaca aaacccacag caaaaattaa ttgaatacca attggaagat agttagatgt
     5881 agtatttgca ttggatgcat ttaataagaa atgatgcata att
//
LOCUS       DAOQHP010000104         5865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7509_length_5865_cov_3.912220, whole genome shotgun sequence.
ACCESSION   DAOQHP010000104 DAOQHP010000000
VERSION     DAOQHP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7509_length_5865_cov_3.912220"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLY81_04625"
     CDS             <1..334
                     /locus_tag="PLY81_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF29541.1"
                     /translation="WLPLGTDFPVEDISPFKTFLAAVIRKDAVGFPANGFQKENALTR
                     EEALRGMTIWAAKANFLENETGSLVVGKKADFILLNNDLMQVPETEILNTKVIATWSD
                     GKKVYNKK"
     gene            complement(347..1252)
                     /locus_tag="PLY81_04630"
     CDS             complement(347..1252)
                     /locus_tag="PLY81_04630"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNF29542.1"
                     /translation="MFAVTILGNNSAIPAYGRMPTAQVVTLNDQQFLIDCGEGTQMQL
                     AKYKIRKSKINHIFISHLHGDHYFGLIGLITSMGLLNRENDLHIYAPAGIEQIIQLQL
                     QVANITLPFSLFFHVLHTEGILINENKFSVECFTVQHSIECWGFIFREKKAPRKIDKE
                     KAVQYQIPAAFYNQLKEGLDYVTKEGLIINNKDVTTTNIPARSYAYCADTKYFEELIP
                     KVKDVNVLYHETTYLKDFEEKAILRFHSTTHQAANIAKQCNAHKLLIGHFSSKYENLD
                     QFLTEAKEVFKSTELAIEGVCYNIL"
     gene            complement(1319..1720)
                     /gene="mce"
                     /locus_tag="PLY81_04635"
     CDS             complement(1319..1720)
                     /gene="mce"
                     /locus_tag="PLY81_04635"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNF29543.1"
                     /translation="MKKVEHIGIAVKDFAISIPLFENLLNVPCYKQETVESENVNTAF
                     FKLGETKIELLQSTNSDGVIAKFIEKKGEGIHHLAFDTDNILAEVERLKNAGFTLLNE
                     TPKEGADNKLVCFLHPKSTNGVLIELCQEKK"
     gene            1846..3072
                     /locus_tag="PLY81_04640"
     CDS             1846..3072
                     /locus_tag="PLY81_04640"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNF29544.1"
                     /translation="MQDMLKLPIYLDNNATTPMDPRVLEAMLPFFTQHFGNAASRNHQ
                     FGWEAEEAVDYAREQVAKLVGADAKEIIFTSGATEADNLAIKGVFEMYASKGNHIITV
                     TTEHKAVLDTCKHIEKVGGEVTYLEVNGDGLIDLTLLEAAIKPTTVLIAIMYANNEIG
                     VVQPIKEIAAIAKKHGILFFTDATQAVGKIPVDVIKDGIDLMAFSSHKMYGPKGIGAL
                     YVRRKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKACEIARLDMEKDTKHIVVL
                     RDKLENALLELEESYLNGNKEHRLPHVSNISFKYVEGEGLMMGFNKNIAVSSGSACTS
                     ASLEPSYVLKALGLGDDLAHSSLRFGLSRFTTEEEIDYTIEAVRNTVLKLREMSPLWE
                     MFKEGIDLDTVEWAHH"
     gene            3161..3580
                     /gene="iscU"
                     /locus_tag="PLY81_04645"
     CDS             3161..3580
                     /gene="iscU"
                     /locus_tag="PLY81_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNF29545.1"
                     /translation="MAYSDKVIDHYSNPKNVGTLDKSSKSVGTGLVGAPECGDVMRLQ
                     IEVDDATGLIKDAKFKTFGCGSAIASSSLATEWLKGKSVDEAVKIDNMDIVEELNLPP
                     VKIHCSVLAEDAIKAAINDYRQKNGLEELTFEQKGVH"
     gene            3585..3938
                     /locus_tag="PLY81_04650"
     CDS             3585..3938
                     /locus_tag="PLY81_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF29546.1"
                     /translation="MISTNENSIYISDKARAKVAKLMEEAGVANDSSYFLRVGVVGGG
                     CSGLSYKLDFDNEVKPMDQVFENDGIKIVTDLKSFLYLVNTELDFSDGLNGKGFYFSN
                     PNASRSCGCGESFAV"
     gene            complement(3940..4638)
                     /locus_tag="PLY81_04655"
     CDS             complement(3940..4638)
                     /locus_tag="PLY81_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29547.1"
                     /translation="MNNTKKAVFNWSGGKDSALALQKVLANNDYEVIALLTTITTGTN
                     KSSVHAIPLHLLQQQAKSIGIKLYVIEVNNDLSNYITEISKAVEIFKQQQVTHFIFGD
                     LSSSNIFNYTKKIFNPLKIEVVEPLYNKTSEEVMEDFLSSGIKAKIIVANYDIFSKDI
                     IGADLNSACVNTMPKGIDICGEQGEYHSFVYEGSIFKTAIKYKIKATKKVAYNIKLDN
                     NKIKTFTYWQAIME"
     gene            complement(4715..5032)
                     /locus_tag="PLY81_04660"
     CDS             complement(4715..5032)
                     /locus_tag="PLY81_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29548.1"
                     /translation="MKIFIVDFSPNNNFLLHFQKEGLHYTIEKNDGGNAYKTVATELP
                     EKIFVNFASKPSHCIQTIKAILNRKTTSKIEIFFVDGDEKNNEKVKELGTSITYHQIK
                     NYI"
     gene            complement(5029..5580)
                     /locus_tag="PLY81_04665"
     CDS             complement(5029..5580)
                     /locus_tag="PLY81_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNF29549.1"
                     /translation="MPKYISLLRGINVSGQKIIKMNELVALYNDVGFNNVTAYIQSGN
                     VVFSTTKTATQKLATRIEEKIKQHYSFEVSVLVLELLTFKSIVEHNPYSIATDKDSKQ
                     LYITFLYKPSVVTTIDALDKIKLPNEEVTIANNAVYFYFAQGYGKTKMNNNFIEQKLK
                     VIATTRNWKTCLQLLKIANTIQP"
     gene            complement(5673..>5865)
                     /locus_tag="PLY81_04670"
     CDS             complement(5673..>5865)
                     /locus_tag="PLY81_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29550.1"
                     /translation="SHTRIWEQLKAEEVEAEKKEAKVQAEQTKKAVKAVQSKVEKATL
                     GDLGALADIKAKLDEAKDK"
BASE COUNT         1994 a          809 c          973 g         2089 t
ORIGIN      
        1 ttggctgcct ttaggtactg attttcctgt ggaagatatt tctcctttta aaactttttt
       61 agcagcagtt ataagaaaag atgcagtagg atttcctgca aatggttttc aaaaagaaaa
      121 tgctttaacc agagaagaag cacttagggg aatgacaatt tgggcagcta aagcaaattt
      181 tctagaaaat gaaacaggaa gtttagtggt aggaaagaaa gccgatttta ttttgctaaa
      241 caacgatttg atgcaagttc ctgaaactga aattttaaat acaaaagtta ttgcaacctg
      301 gagtgatggc aaaaaagttt ataataaaaa ataactttaa ctaagtctat aaaatattat
      361 aacaaacacc ttcaatagct aattcagtag atttgaaaac ttcttttgct tctgttaaaa
      421 attggtctaa attctcatat ttactactaa aatgcccaat taataattta tgtgcattgc
      481 attgtttggc aatgttagct gcctggtgtg ttgtagaatg gaaacgtaaa attgcctttt
      541 cttcaaaatc ttttaaataa gttgtttcgt ggtataatac atttacatct ttcactttag
      601 gtatcaactc ttcaaaatat tttgtatcag cacaataggc ataagacctg gcaggtatat
      661 tagttgtagt tacatcttta ttattaataa ttaaaccttc tttagttaca taatctaacc
      721 cttcttttaa ttggttgtaa aaggctgcag gaatttgata ttgaactgct ttttctttat
      781 caatttttct tggtgctttc ttctctctaa aaataaagcc ccaacattca atactgtgtt
      841 gtactgtaaa acattcaaca gaaaatttat tttcattaat taaaattcct tctgtatgca
      901 atacatgaaa aaataaagaa aatggtaagg tgatattggc aacttgtaat tgtagttgta
      961 taatttgttc tattccagca ggtgcataaa tgtgtaaatc attctctcta tttaataagc
     1021 ccatgcttgt aattaaacca attaaaccaa agtaatggtc gccatgtaaa tgagaaatga
     1081 aaatatgatt aatcttgctt tttcttattt tatatttagc aagttgcatt tgtgttcctt
     1141 cgccacagtc aattaaaaac tgttggtcgt ttaaagtaac aacttgagca gttggcattc
     1201 tgccataagc aggtattgct gaattgtttc ctaaaattgt tactgcaaac attttaaaaa
     1261 tataattaga ttatggctta ataattattt tatttatatc atttaaacag taggtatatc
     1321 attttttttc ttggcataat tctattaaaa caccattagt acttttagga tgcagaaaac
     1381 aaaccaattt attatctgca ccttctttgg gtgtttcatt taacaaagta aagccagcat
     1441 tttttaacct ttctacttcc gccaaaatat tatcagtatc aaaagctaag tgatggatgc
     1501 cttcgccttt tttctcaata aattttgcaa ttacgccatc agaatttgta ctttgcaaaa
     1561 gctcaatttt agtttcgcca agtttaaaaa atgcagtgtt aacattttct gattctactg
     1621 tttcttgttt ataacagggt acatttaata aattttcaaa taatggtatg ctaatggcaa
     1681 aatctttaac tgcaatacca atatgttcta cttttttcat aaaaaatatt taataatttt
     1741 tattctactt acgatatatg gaaattgaaa ttcataaagc tgttatctct taaaaagaag
     1801 cctaaaaaat agtacatttg tggcatattt ttttaaattt tttaaatgca agatatgttg
     1861 aagttgccaa tttatttaga taataatgct accactccaa tggatccaag agtgttagaa
     1921 gctatgctgc ctttttttac acaacatttt ggtaatgcag ccagcagaaa ccaccaattt
     1981 ggatgggagg cagaagaagc tgtggattat gcaagggagc aagtagcaaa attagtaggt
     2041 gcagatgcta aggaaataat ttttacaagt ggtgctaccg aagcagataa tttagccatt
     2101 aaaggtgtgt ttgaaatgta tgcaagtaaa ggcaaccata ttataacagt taccactgaa
     2161 cataaagcag tattagatac ttgtaaacat attgaaaaag taggaggtga ggtaacctat
     2221 ttagaagtaa atggagatgg gttaatagac cttacattat tagaagctgc aattaaacca
     2281 actactgttt taattgcaat tatgtatgct aacaatgaaa ttggtgttgt acaacctata
     2341 aaagaaattg ctgcaatagc caaaaaacat ggcattttat tttttacaga tgctacacaa
     2401 gcagttggta aaatacctgt tgatgtaata aaagatggta tagatttaat ggcattcagc
     2461 agccataaaa tgtatggccc aaaaggtatt ggtgctttat atgttagaag aaaaaatcct
     2521 agagtaaaag taactgcaca aatggatggt ggaggccatg aaagaggtat gagaagtggc
     2581 acgttaaatg taccaggaat tgtaggtttt ggtaaagcct gcgaaattgc aagattagat
     2641 atggagaaag ataccaaaca tatagtagta ttaagagata aattagaaaa tgccttattg
     2701 gaattagaag aatcttattt aaacggaaat aaagaacatc gattaccaca tgttagtaat
     2761 atttctttta agtatgtaga aggagaagga ttaatgatgg gctttaataa aaatattgca
     2821 gtaagttcag gttctgcctg cacttctgca tctcttgaac caagttatgt gttaaaagct
     2881 ttaggcttag gcgatgattt agcccacagc tctctacgtt ttggattaag cagatttaca
     2941 acagaagaag aaatagatta taccattgaa gcagtgagaa atactgtgtt gaagctgaga
     3001 gaaatgagtc cgttatggga aatgtttaaa gaaggtatag atttggatac tgtagaatgg
     3061 gcacaccact aaaacaaaat agtattgtgt ttttaacaaa ataaattaga gcagagaaac
     3121 taaaaatttt ttataatcgt aatttaaaaa tatcaattat atggcatatt cagataaagt
     3181 aatagaccac tatagtaacc ctaaaaatgt tggcacttta gataaaagta gtaaaagtgt
     3241 aggaacaggt ttagtaggtg caccagaatg tggagatgta atgcgtttac aaattgaagt
     3301 ggatgatgca acaggattaa ttaaagatgc aaaatttaaa acatttggtt gcggctcagc
     3361 tattgcatcc tcctcattag caacagaatg gttaaaagga aaatcggtag atgaagcagt
     3421 aaaaatagat aatatggata ttgtggaaga attgaattta ccacctgtga aaattcattg
     3481 ttctgtatta gcagaagatg caattaaagc tgcaattaat gattatcgtc aaaaaaatgg
     3541 cttagaagaa ttaacttttg agcaaaaagg agtacactaa ttttatgata tctactaatg
     3601 aaaatagtat ttacataagt gataaagcta gagctaaagt tgcaaaacta atggaagagg
     3661 ctggtgttgc taatgattca tcttatttct taagagtagg agtagttggt ggtggctgta
     3721 gtggcttaag ctataagtta gattttgata atgaggtaaa acctatggac caggtttttg
     3781 aaaatgatgg aattaaaatt gttaccgacc ttaaaagctt tttatactta gtaaatactg
     3841 aattagactt tagtgatggg ttaaatggaa aagggtttta ttttagtaat cctaatgcaa
     3901 gcaggagctg tggttgcgga gaaagttttg ctgtttaatt tattccatta ttgcttgcca
     3961 ataagtaaaa gtttttattt tattgttatc taatttaata ttgtaagcca cttttttagt
     4021 ggctttaatt ttatacttaa ttgctgtttt aaaaatactt ccttcgtaaa caaaactgtg
     4081 gtattcacct tgctctccac aaatatcaat acctttaggc atggtgttta cacaagctga
     4141 gtttaaatct gcacctataa tatctttact aaaaatatcg tagttggcca caataatttt
     4201 tgctttaata ccggaggata aaaaatcttc cataacttct tcagaagttt tgttataaag
     4261 tggttctacc acttcaatct taagtgggtt aaaaattttc ttcgtgtagt tgaatatgtt
     4321 tgaactggat aaatcgccaa aaataaaatg agttacttgt tgctgtttaa atatttcaac
     4381 ggccttgctt atttcggtaa tgtaattact taagtcatta tttacctcaa taacatataa
     4441 tttaatacct atactttttg cttgttgttg taataaatgc aatggtatag catgtaccga
     4501 agatttattt gtacctgtag tgatagtggt taaaagtgct ataacttcat aatcgttatt
     4561 tgctaaaact ttttgtagtg ctaaagcaga atcttttcca ccactccaat taaaaacagc
     4621 tttcttagta ttattcatct attaaaaata tgctactcaa gtaaaataaa atatttgttg
     4681 cttggtatat aatacaagca ttcattttac aactttaaat atagtttttg atttgatggt
     4741 atgtaattga tgtacctagc tccttaactt tttcgttgtt tttttcatct ccatctacaa
     4801 aaaatatttc aatttttgat gtagtttttc tgtttaaaat tgcttttata gtttgtatgc
     4861 aatggcttgg tttgcttgca aaatttacaa atattttttc agggagttct gtggcaacag
     4921 ttttgtacgc attgcctcca tcattttttt caatagtata atgaaggcct tctttttgaa
     4981 aatgaagtaa gaaattatta tttggcgaaa aatctacaat aaaaattttc atggttgaat
     5041 tgtattggca atttttaaaa gttgtaagca ggttttccaa ttacgagttg tagctattac
     5101 tttcaatttt tgttcaataa aattattgtt catttttgtt ttaccataac cttgtgcaaa
     5161 ataaaagtac accgcattat tagcaatcgt aacttcttcg tttggtagtt ttattttatc
     5221 taatgcatca atagttgtaa ctacggaagg tttgtacaaa aatgttatat acaattgctt
     5281 actatcttta tcagttgcaa tagaataggg gttgtgctca acaatacttt taaaagtcaa
     5341 taactctaaa accaatacag aaacttcaaa tgaataatgt tgttttattt tctcttcaat
     5401 tctggtggct agtttttggg tagcagtttt agtagttgaa aaaactacat taccactttg
     5461 aatataggca gttacattat taaaacctac atcattgtat agtgcaacca attcattcat
     5521 tttaataatc ttttggccac ttacatttat tcctctcaat aatgaaatat atttcggcat
     5581 tcttttttta atgtaaagta ttcattcaaa tatataaaaa aaacagcccc aataatgcaa
     5641 ggggctgttg aagaaagtat atctaaaatt atttacttgt cttttgcttc atcaagttta
     5701 gctttaatat cagctaaagc acctaaatct cctaaagtag ctttttcaac tttagattga
     5761 acagctttta cggctttttt agtttgttca gcttgtactt ttgcttcttt cttttctgct
     5821 tctacctctt ctgcttttaa ttgttcccaa atacgagtat ggctt
//
LOCUS       DAOQHP010000105         5862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7514_length_5862_cov_3.792147, whole genome shotgun sequence.
ACCESSION   DAOQHP010000105 DAOQHP010000000
VERSION     DAOQHP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_5862_cov_3.792147"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLY81_04675"
     CDS             <1..593
                     /locus_tag="PLY81_04675"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29551.1"
                     /translation="VQDYRSTYNDIRDWLRREKSGKETEESTIDWDDVVFEVDLLKSQ
                     EINLDYILELIFEHNKKTKDKDTLITEIRRVIRASVGNRAKESLVVDFINETDLDTIQ
                     DKANVIDSFFVFAQNKQKTEALELITEENLNVEEAKRYITTSLKREFASENGTELNAL
                     LPKMSPLNPQYLTKKQSVFQKLVSFVEKFKGVGGQL"
     gene            909..2339
                     /locus_tag="PLY81_04680"
     CDS             909..2339
                     /locus_tag="PLY81_04680"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNF29552.1"
                     /translation="MKYLSICLFILSNIFFTHVFAQNSTSNFKKLNIINTTNDSGFLT
                     IGSININGNKRTKDYIILREVPFKLGNKYKPHELTGLLELARNQIMNTALFVDAQVYV
                     TNLLKDTAFINIDVKERWYIFPIPYFNLVDRNFNQWINEHKASLDRVNYGIKFFHFNV
                     SGRNDKLSLDVIGGYSQQLQFNYTNPFADKTLKHGFSVGFGYVRQKELNYASLNNKQV
                     FVRTNSFVRDGFRSLLTYSYRPDSKYRFYVSLGLNMDKISDTVLKLNPTYFPYYTNKI
                     TYPTLLVMLQYFGVDYIPFPKKGFLYQVSMFNRSFNKKANLFQLSVKTTNVLPLATNS
                     FLNIHTNSILKIGDNRSYFNQSLMGYNEVFLRGLESYVIDGMAGVVANNSLYQKLFKF
                     VFKNPIKIKGHENIPFHFYAKLFSDFGYAYNKYAAVNNPLANKFLYTAGAGIDITTIY
                     DFVFRIEYGLNQLGNTAFGLRVKGEF"
     gene            complement(2340..3413)
                     /locus_tag="PLY81_04685"
     CDS             complement(2340..3413)
                     /locus_tag="PLY81_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNF29553.1"
                     /translation="MRLFVFSFLSILILSNVAFTQPNKQDDNLFAAIKEKDVKKFHKA
                     IKKGANINARNSQNFTPLHAIFEYNNYGLGSAENEMFETLIQLGADVNAEINSKYHNT
                     PLVSAIRANACKYIEPLIKAGANVKVGRLLHLACFKTSYDCVEILVKNGLDVNAVDDL
                     NETPLIALLTNNDPYNKTIKILEYLHKKGADIFILNKEGKSLVDLCNRWNNTQCSNYL
                     MKNGVVARIKPSTSSENNGDKKVRTSFSRNYYGKTYSNGKTVEMYANISVSPNEIKIY
                     NEEGSLLHKCTVIKSKYIKENGIDGIEYYTKHQSGSNLYIYFYEMPNFTYYSNGVQAS
                     ITGWNRCVLKNPGESSLILDAQW"
     gene            complement(3435..5117)
                     /locus_tag="PLY81_04690"
     CDS             complement(3435..5117)
                     /locus_tag="PLY81_04690"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNF29554.1"
                     /translation="MKIFLAQQNYHIGNFELNTQKIIQAIEEAKQKGGDLIVFSEMCV
                     CGYPARDFVEFEDFINKCYHAINKIKEHADSIGVLIGSPARNKIKEGKDLFNASFFLY
                     EKEIKAEVHKTLLPTYDVFDEYRYFEPAYNWNTIDFKGKKIAVTICEDIWNLGDNPLY
                     RICPMDELMKQEPDVMINLSASPFDYTHIEDRKAIIKANVLKYKLPLIYCNTVGSQTE
                     IIFDGASLVFDKNANLIKALPVFKETIDCVELNNDGTFNASVIEMANQISNKELNPST
                     LNEQLNIAQIHDALVLGIKDYFSKMNFTKAILGSSGGIDSAVVLTLACQALGAENVRA
                     VLMPSPYSTEHSVNDAVQLSKNLNNPYDIIRIDNVYESFLSTLIPIFNNLPFSLAEEN
                     LQSRTRGNLLMAIANKFGYILLNTSNKSELATGYGTLYGDMAGGLGVLGDCYKLQVYA
                     LANYINRDKEIIPINIINKAPSAELRPEQKDSDSLPDYTILDNILYQYIEMRLGPSEI
                     KALGFEAATVDRILKMVNNNEYKRNQFCPIIRISPKAFGMGRRMPIVGKYLS"
     gene            5260..>5862
                     /gene="meaB"
                     /locus_tag="PLY81_04695"
     CDS             5260..>5862
                     /gene="meaB"
                     /locus_tag="PLY81_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNF29555.1"
                     /translation="MFHQLLQQIQQGNIKSLARCISLIENEVDGYELFLKQLPYSTTK
                     IIGITGPPGAGKSTLTDALISEIIAQQKKVAVVCVDPSSPFNLGALLGDRIRMSEWYN
                     NENVFIRSLATRGSLGGLHPKIIEITDILKAAPFDYIIVETVGVGQSEIEIVGLADTT
                     IVVVVPEAGDEVQTMKAGLMEIADIFVVNKADRPDADLFVK"
BASE COUNT         1996 a          874 c          864 g         2128 t
ORIGIN      
        1 cagttcagga ttaccgttca acttacaatg atattcgtga ttggttaaga cgagaaaaaa
       61 gtgggaaaga aacagaagaa tcaacaatcg actgggatga tgtagttttt gaagttgact
      121 tactcaaatc acaagagata aaccttgact atattctaga attgatattt gagcataata
      181 agaaaactaa agacaaagac actttgatta ccgaaatacg cagagttatt cgtgcaagtg
      241 taggaaatag agccaaagaa agtttggttg ttgatttcat caatgagact gaccttgaca
      301 caattcaaga caaagccaat gtgatagatt cattttttgt atttgctcaa aacaagcaga
      361 aaacggaagc attggaatta atcacagaag aaaatctgaa tgttgaagaa gcaaaacgat
      421 acataacaac ttcattaaag cgagaatttg caagtgaaaa cggaacagaa ctcaatgcac
      481 ttttaccgaa aatgagtcca ttaaatcctc aatacttgac aaaaaagcaa agcgtttttc
      541 aaaagttagt ttcgtttgta gagaaattta aaggagttgg aggacaactg taaaaaacta
      601 aaagtaaaat ttgagcattt aaatgtttta tgacattact tccaatggca actgtatttg
      661 aaatttaaaa gacatattga ttaaagcaga aattacattt tatatataag aacattactt
      721 ttttaaaaac tttcctaagc ttctatatta aatagctaaa attagagtaa ggttaaccca
      781 ccatttttta taaaataggc ttgttctttt tcattttttg ttttatttta tagaattgag
      841 caagttcctt tttatttcca ttctacaaaa acctattttt ataactttat taatcttgtt
      901 ttttattaat gaagtattta tccatctgct tatttatttt atctaatatt ttttttacgc
      961 atgtttttgc ccaaaatagt acatctaact ttaagaaact taatataata aataccacaa
     1021 acgattctgg atttttaact attggctcaa ttaatattaa tggcaataaa agaacaaaag
     1081 attatataat attaagagaa gtgcctttta aattagggaa taaatacaaa ccacatgaac
     1141 ttacagggtt gttagaattg gcaagaaatc aaataatgaa cactgcacta tttgtagatg
     1201 cacaagttta tgtaacaaat ttattaaaag atactgcttt tataaatatt gatgtgaaag
     1261 aaaggtggta catttttcct attccttatt ttaatttggt tgatagaaat tttaatcagt
     1321 ggattaatga gcataaagca agccttgata gggtgaatta tggaataaaa ttttttcatt
     1381 tcaatgttag tggtagaaat gataaacttt cgttagatgt gattggaggc tatagccaac
     1441 agctacaatt taattatacc aacccttttg cagataaaac tttaaagcat ggtttctctg
     1501 ttggttttgg ttatgtaagg caaaaagagt taaactatgc ctctttaaat aataagcagg
     1561 tatttgtacg taccaatagt tttgttagag atggatttag aagcttactc acttattctt
     1621 atcgcccaga ttctaaatat cgtttttatg ttagcttagg cttaaatatg gataaaattt
     1681 ctgacacagt tttaaaatta aaccctactt attttcccta ctataccaat aaaattacat
     1741 atcctacctt attggtaatg ctacaatatt ttggggtaga ttatattcct tttccaaaaa
     1801 aaggtttttt gtatcaggta agcatgttta atagaagttt taacaaaaag gcaaaccttt
     1861 ttcagttgag tgtaaaaaca accaatgtat taccattagc tacaaatagt tttttgaaca
     1921 tacatactaa tagcatttta aaaattggag ataatagaag ttattttaac caatcgttaa
     1981 tggggtataa cgaggttttt ttaagaggct tagagtctta tgttattgat ggaatggctg
     2041 gcgttgtagc caacaattcc ttgtatcaaa aactatttaa gtttgttttt aaaaatccta
     2101 ttaaaataaa agggcatgaa aatattccat ttcattttta tgcaaaactg tttagcgatt
     2161 ttgggtatgc ttataataaa tatgcagcag ttaataaccc tttagcaaat aagtttttat
     2221 atactgctgg tgcaggaatt gatattacta ctatatatga ttttgttttt agaattgaat
     2281 atgggctaaa tcaattaggt aatacagctt ttggacttag agttaaagga gagttttaac
     2341 taccattgtg catctaatat taaagaactc tcccctgggt tctttaatac acaacggttc
     2401 cagcctgtaa tacttgcttg tacaccattg ctataatatg taaaattggg catttcataa
     2461 aagtatatgt ataaattact tccagattga tgttttgtgt aatattcaat accatcaatg
     2521 ccgttttctt ttatgtattt gctttttatt acagtacatt tatgaagtaa agaaccctct
     2581 tcattatata tttttatttc attaggacta acagaaatgt tagcatacat ttctactgtt
     2641 ttaccattac tataggtttt gccataataa tttctactaa aagaagttct tacttttttg
     2701 tctccattat tttcactcga agtggaaggt tttatacgag ccactactcc atttttcatt
     2761 aaataatttg aacattgtgt attgttccat ctgttacaca aatcaactaa actttttcct
     2821 tctttgttta atatgaaaat gtctgcacct tttttatgta aatactctag aatttttatt
     2881 gttttattat agggatcgtt gttagtaagc aatgcaatta aaggtgtttc atttaagtca
     2941 tctactgcat ttacatctaa gccatttttt accaatattt ctacacaatc ataagatgtt
     3001 ttaaaacacg ctaggtgtaa taaacgacca acttttacat tagcaccggc ttttattaaa
     3061 ggctctatgt atttacaggc atttgcacga attgcagata ccaaaggagt attatggtat
     3121 ttactattaa tttctgcatt tacatcagct cctaattgta taagtgtttc aaacatttca
     3181 ttttctgcac ttcctaaccc ataattatta tattcaaaaa tggcatgtaa gggtgtaaaa
     3241 ttttgactgt ttcttgcatt aatattggct ccctttttaa tagctttatg aaactttttt
     3301 acatcttttt cttttatggc agcaaacaaa ttatcatctt gtttattggg ctgtgtaaaa
     3361 gcaacattgc ttaaaattaa aatagataaa aaactaaaaa caaataaacg catattaaaa
     3421 taaattttta aaaatcaact taaatattta cctacaatgg gcattcttct acccatgcca
     3481 aaagctttag ggcttattct aataattggg caaaactgat ttcttttata ttcattgttg
     3541 tttaccatct ttaaaattct gtccacagtt gcagcctcaa agcccaatgc ttttatttca
     3601 gatggtccta atctcatttc aatatactga tacaaaatat tatctaaaat ggtataatcc
     3661 ggtaagctat cactatcttt ttgttcaggt cttaactctg cacttggtgc tttgtttata
     3721 atatttatag gaataatttc tttatctcta tttatataat ttgccaaagc atacacttgc
     3781 aatttatagc aatctcctaa aacgcctaaa ccaccagcca tgtcaccata taatgttcca
     3841 tagcctgttg ctaattcact tttattgcta gtatttaaaa gaatataacc aaatttgttt
     3901 gcaatggcca ttagtaaatt tcctctggta cggctttgta agttttcttc tgcaaggcta
     3961 aaaggtaaat tattaaatat aggaattaat gtagaaagaa aactttcata aacattgtct
     4021 attctaatta tatcataagg gttatttagg tttttactta gctgtactgc atcatttaca
     4081 ctatgctcag tactatatgg cgatggcatt aaaactgctc ttacattttc ggcaccaaga
     4141 gcttggcatg ctaaagttaa tactactgca ctatcaattc caccactact tcctagaatg
     4201 gcttttgtaa aattcatttt agaaaaataa tctttaatcc ccaatactaa agcatcgtgt
     4261 atttgagcaa tgtttaattg ttcatttaag gtagatggat ttaattcttt attagaaatt
     4321 tgattagcca tttcaataac agatgcattg aaagttccat cattgttgag ttctacgcaa
     4381 tctattgttt ctttaaaaac aggtaatgct ttaataagat ttgcattttt atcaaataca
     4441 agacttgctc catcaaaaat aatttctgtt tgactaccta ctgtattgca ataaatcaaa
     4501 ggtagtttgt atttcaaaac attggcttta ataattgctt ttctatcttc aatatgagtg
     4561 taatcaaaag ggcttgcact taggttaatc attacatctg gctcttgctt cattaattca
     4621 tccatggggc aaattctata caatggatta tcacccaaat tccaaatgtc ttcgcaaatg
     4681 gttactgcta ttttttttcc tttaaaatct atggtattcc aattataagc aggttcaaaa
     4741 tatctgtatt cgtcaaacac atcatacgtt ggcagtaagg ttttatgtac ttctgctttt
     4801 atttcttttt catacaaaaa gaaagaagca ttgaataaat cttttccttc tttaatttta
     4861 tttcttgctg gtgagccaat taaaacacca atactatcag catgttcttt tattttgttt
     4921 attgcatggt aacatttatt aataaagtct tcaaactcta caaaatctct agcagggtag
     4981 ccacaaacac acatttcgct gaatacaatt aaatcgccac ctttttgttt agcctcttct
     5041 atggcctgaa taattttttg tgtattcaat tcaaaatttc caatatgata attttgttga
     5101 gctaaaaata ttttcatggt acaaatttca tatttataca aatacaaaaa agttatttaa
     5161 cagatagagt tactttaatg aaaatttaaa atagttgtaa gctatttatc ttttattgct
     5221 attctaagta aattcgtaca ttaaatacaa ttaccaaaaa tgtttcatca actattacaa
     5281 caaattcaac aaggcaatat taaatcttta gcacgttgta tttctttaat agaaaatgaa
     5341 gtggatggat acgaattgtt tttaaagcag ttgccttatt ctactacaaa aattattggc
     5401 attacaggcc cacctggtgc aggaaaaagt actttaaccg atgctttaat tagcgaaata
     5461 attgcacagc aaaaaaaggt tgctgtagtt tgtgtagatc cttcatcgcc ttttaattta
     5521 ggtgctttgt taggcgatag aattagaatg agtgaatggt ataacaacga aaatgtattt
     5581 atacgttctt tagcaacaag aggcagtttg ggaggcctac atccaaaaat tattgaaata
     5641 acagatattt taaaagctgc tcctttcgat tatattattg tagaaacagt gggcgttgga
     5701 caaagtgaaa ttgaaatagt aggattggca gatacaacca ttgtagttgt agtgccagag
     5761 gctggcgatg aagtgcaaac tatgaaggca ggcttaatgg aaattgcaga tatttttgtt
     5821 gtaaataaag cagataggcc agatgcagat ttatttgtaa aa
//
LOCUS       DAOQHP010000106         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7616_length_5808_cov_2.930645, whole genome shotgun sequence.
ACCESSION   DAOQHP010000106 DAOQHP010000000
VERSION     DAOQHP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_5808_cov_2.930645"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLY81_04700"
     CDS             complement(<1..911)
                     /locus_tag="PLY81_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNF29556.1"
                     /translation="MSNENEIKHHTDSGQCPYHQVAKNALPTGSNKEIKKDWWPNQLN
                     LQVLRQNSSLSNPMDKDFDYAQAFNQLDYFALKADLAKLMTDSQEWWPADFGNYGPFF
                     IRMAWHSAGTYRIGDGRGGAGTGQQRFSPLNSWPDNGNLDKARRLLWPIKQKYGSKIS
                     WADLMILTGNVALETMGFKTLGFAGGRADVWEPESDIYWGSETKWLATSKEANSRYSG
                     ERDLENPLAAVQMGLIYVNPEGPDGNSDPLAAAVDMRETFARMAMNDEETVALIAGGH
                     TFGKTHGAGDVALVGPEPEAAPIEAQG"
     gene            1023..1973
                     /locus_tag="PLY81_04705"
     CDS             1023..1973
                     /locus_tag="PLY81_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF29557.1"
                     /translation="MTLIQLEYIIAVSNLKHFAKAANFCNVTQPTLSMQIQKLEEEFE
                     VKIFDRSKQPVIPTILGEKIIEQAKRIIAEKENLSQLLIQTKGETAGELKIGIIPTLA
                     PYLLPLFLNKFIKKYPKVKLKITELNTNSIIKKLKENTIDIGILVTPLNEKVISETPL
                     FYEELMAYISKRNKEFNKTYMLTKDIDIKKLWLLEEGHCFRTQVLNLCELRKNMNGES
                     QFEYAAGSFETLRKMVDVTDGLTILPELATEYFSASEMQRIRHFKAPAPVREISIVLH
                     RNYAKQQLVTILKDEILNAIPEKIKKNKKTFIVPFESKES"
     gene            1999..2508
                     /locus_tag="PLY81_04710"
     CDS             1999..2508
                     /locus_tag="PLY81_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29558.1"
                     /translation="MKKILILLFVMAFNYNSFSQGIATESAKQFYNKIFEYDYVEKKP
                     IFVAGRDSLRNFYLSHFNAFDSIVTRAIIKGDTAKYLRIHFEYIIDENGVPYNSQFKY
                     IGSTRYGSSSGDKKLKYFNDLNSYFKEATKTMIHKMPNWRPALQNNIRVKCNVEDYFQ
                     IWLGINPEK"
     gene            2539..4323
                     /locus_tag="PLY81_04715"
     CDS             2539..4323
                     /locus_tag="PLY81_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29559.1"
                     /translation="MKILIRYIKPYKWLIALAFLLATINQIFSLLDPLIVGKVIDLFA
                     NHPKHFDTNKTLLRTENEFLFGSSTFHGLIFFILLLIGTAMVSRVAKAFQDYTVNVII
                     QKFGAGIFTDGLKHSMQLPFQEFEDQRSGETLSILTKVRTDTEKFISYFINVFLSIII
                     SIVFVSIYAFKLHWSIVPVYMGGSILIGFTTSLLSKKIKNIQKNIVKETTVLAGSTTE
                     SLRNIELVKSLGLTNEEVKRLNNNTYKILGLELKKVKSLRTLSFIQGTMINFLRQVIT
                     FTLLWLIYKDVLTTGQFISLQFYSFFIFGPMQEIGNIIISYREAQASLLNFDELMKKK
                     PEPKPTSPLHLGIIKEVSFEHISFKHTSAKYKALDDISFKVNEGETVAFVGPSGSGKS
                     TLVKLLVGLYTPQEGNIKYNNLYSKEIDFDELRTQIGFVTQDTNLFAGTIKENLLFVN
                     SSATNDELNDVLEKASCQNLLERADNGINTVIGEGGLKLSGGEKQRISIARALLRKPH
                     LLIFDEATSALDSITEEEITNTIKQISLHKKQVTIMIAHRLSTIMHADRIFVLEKGKI
                     VETGNHQELLNEKGLYFAMWRQQIGERK"
     gene            complement(4334..5440)
                     /locus_tag="PLY81_04720"
     CDS             complement(4334..5440)
                     /locus_tag="PLY81_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNF29560.1"
                     /translation="MSAILSIFNDFFIHSFWMKVLKLYNKKVETKFYFKFCFFILFGL
                     GIYQNSNAQVATVDKIKMYAPYRFNLLTEQNFFDSLSQNTPSHLRNYKRIRLVSAVNI
                     VGYGGAMIGLYNAWYKNYPQSNFHFFNDNEEWLQVDKAGHAYSAYVESKASMELWRWA
                     GLPRKQRIWLGGLSGATYQTAIEFLDAYSVGWGWSWGDFIANCYGSGLLIAQELLWDE
                     QRVDLKFSFHKVNYKDPFLHNRADKLFGKSFLNRMLKDYNGQTYWLSANIKSFFPKSN
                     VPSWLNVAVGYGADGMFDGDDNIWKDENNNVIDRSDIPRYRQWYIAPDINLTKIKTKS
                     KFLKVALFFLNSFKFPAPSIGFSKKGVEFNWLHF"
     gene            5512..>5808
                     /locus_tag="PLY81_04725"
     CDS             5512..>5808
                     /locus_tag="PLY81_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNF29561.1"
                     /translation="MDNSISVKVNEWLGGNYDETTKQIIKNLQAENELELTESFYKNL
                     EFGTGGLRGIMGVGTNRMNKYTVGMATQGFANYLKKVYGQEEIKIVIGHDSRNNS"
BASE COUNT         2162 a          897 c          861 g         1888 t
ORIGIN      
        1 aaaccttgtg cttctatagg tgctgcttca ggttcagggc ctaccaaagc aacatcacct
       61 gcaccatgag ttttaccaaa agtatgccca cctgcaatta atgcaacagt ttcttcatcg
      121 ttcatagcca ttcttgcaaa agtttctctc atatcaactg ctgctgctaa tggatcagag
      181 ttcccatctg ggccttcagg gtttacataa atcaagccca tttgtactgc agctaaggga
      241 ttttctaaat ctctttcacc tgagtatcta ctattggctt ctttactagt tgccagccat
      301 tttgtttcac taccccaata aatatcagat tctggctccc aaacatcggc tctaccacct
      361 gcaaaaccca aagttttaaa acccattgtt tcaagagcta cattacccgt taaaatcatt
      421 aaatctgccc aggaaatttt gctaccgtat ttttgtttaa taggccataa caatcttcta
      481 gctttatcta aattgccatt atctggccat gaatttaaag gcgaaaatct ttgttgccct
      541 gtacctgctc cacctcttcc atctcctatt ctataggttc ctgcactatg ccatgccata
      601 cgaataaaga aaggtccgta atttccaaaa tctgctggcc accattcttg agagtctgtc
      661 attaatttag ctaaatcggc ttttaaagca aaataatcta attgattgaa agcttgtgca
      721 tagtcaaaat ctttatccat ggggttagat aaagaagagt tttggcgtaa gacttgtaag
      781 tttaattgat taggccacca atctttttta atttctttat tactacctgt aggtaaagca
      841 ttttttgcca cctgatggta agggcattga ccactatcag tatggtgttt tatttcgttt
      901 tcattgctca taaaaaatta ttttgaggtt gattaattat agaatgcaac aaacttattg
      961 aaaattattg tatcaataaa atcaattaac tttatataat catagataaa acctattaac
     1021 aaatgaccct tattcagtta gaatatatta tagcagtaag taatttaaaa cactttgcaa
     1081 aagcagcaaa tttttgtaat gttacacagc caacattaag catgcaaata cagaaattag
     1141 aagaggaatt tgaagtgaag atttttgata gaagtaagca accagttata cctacaatat
     1201 tgggtgaaaa aattattgaa caagccaaaa gaattatagc agaaaaagaa aacttaagcc
     1261 aattattaat acaaacaaaa ggagaaactg ctggggagtt aaaaattgga attattccta
     1321 cacttgcacc ctacttattg cctttgtttt taaataaatt tattaaaaaa tatccaaaag
     1381 ttaaattaaa aataaccgaa cttaacacta atagtataat aaagaaatta aaagagaata
     1441 caatagacat aggcatttta gtaaccccac ttaacgagaa agtaatttcc gaaactccct
     1501 tattttacga agagttaatg gcatatattt caaaaagaaa taaggaattt aataaaactt
     1561 atatgctaac aaaagatata gatataaaaa aattatggct tttagaagaa gggcactgct
     1621 ttagaaccca ggttttaaat ctttgcgaat taagaaaaaa tatgaatggc gaaagccaat
     1681 ttgaatatgc tgctggcagc tttgaaactt tgagaaaaat ggttgatgta accgatgggt
     1741 taaccatttt acctgaattg gcaacagaat attttagtgc tagcgaaatg cagagaataa
     1801 ggcactttaa agctcctgca cctgtaagag aaattagtat tgttttacat agaaattatg
     1861 caaagcaaca attggtaaca attttaaaag atgaaatttt aaatgctatt ccggaaaaaa
     1921 ttaaaaaaaa taaaaaaaca tttattgttc catttgaaag taaagaatca taaatttaat
     1981 tttaaaaaat attgcaaaat gaagaagata ctgatattgc tatttgttat ggctttcaac
     2041 tacaattctt tttcgcaagg aattgcaacc gaaagtgcca aacaatttta taataaaatt
     2101 tttgagtatg actatgtaga gaaaaaacca atatttgttg ctggcagaga ttctttaaga
     2161 aatttttatt tatcgcattt taatgctttt gattcaatag ttaccagagc cattataaaa
     2221 ggtgatacag caaagtactt aaggatacat tttgagtata taattgatga aaatggtgta
     2281 ccatacaatt ctcaatttaa atatattggt agtacaagat atggcagttc atctggagat
     2341 aaaaaattaa aatattttaa cgaccttaac tcgtatttta aagaagctac aaaaacaatg
     2401 atacataaaa tgcctaattg gcggccagcc ttacaaaata atataagagt aaaatgcaat
     2461 gtggaagatt attttcaaat ttggttaggt atcaatccag aaaaataaca aaaccatcga
     2521 actgcaattt taaaacaaat gaaaatttta atccgctaca ttaaacctta taaatggctt
     2581 atagcactag cttttttatt agcaaccatt aatcaaatct tttcactctt agacccactc
     2641 attgttggaa aagtaattga tttatttgcc aatcatccaa aacattttga caccaataaa
     2701 actttattaa gaacagagaa tgaattttta tttggcagtt caacttttca tggattaatc
     2761 ttttttattt tattgcttat tggcactgca atggtaagta gagttgcaaa agcctttcag
     2821 gattatacag tgaatgttat tattcaaaaa tttggagcag gcatatttac agatggacta
     2881 aaacacagta tgcaattgcc atttcaagaa tttgaagacc aacgaagtgg tgaaacatta
     2941 tctattttaa ctaaagtaag aacagataca gaaaaattta tttcctattt tataaatgta
     3001 tttttaagca taattatcag cattgttttt gtttctattt atgcttttaa acttcattgg
     3061 tctattgtac ctgtatatat gggaggctct attttaattg ggtttaccac tagtttattg
     3121 agcaaaaaaa ttaaaaatat tcaaaaaaat attgtaaaag aaactactgt tttagcaggc
     3181 tctactacag aaagtttaag aaatatagag ttagttaaaa gtctaggttt aaccaacgaa
     3241 gaagttaaaa ggcttaacaa taatacctat aaaattttag ggcttgaact aaaaaaagta
     3301 aaaagtttaa gaactctaag ttttattcaa ggtactatga ttaatttttt aaggcaggta
     3361 attacattta ctttgttatg gttaatttat aaagatgttt taactacggg tcaatttata
     3421 tcccttcagt tttatagttt tttcattttt ggccctatgc aagaaattgg caatattatt
     3481 attagttaca gagaagctca ggcatcttta ttaaactttg atgagttgat gaagaaaaag
     3541 ccagagccta aaccaacctc acctttacat ttaggtatta ttaaagaagt tagttttgaa
     3601 catatatctt ttaaacacac ctctgcaaaa tacaaagcac tagatgatat ttcttttaaa
     3661 gtaaatgagg gagaaacagt tgcttttgtt gggccaagtg gtagtggtaa aagtacttta
     3721 gttaaattat tagttggttt atacacccca caagaaggga atattaaata caataattta
     3781 tacagcaaag aaatagattt tgatgaactt agaacacaga ttggttttgt aactcaggat
     3841 accaatttat ttgcaggaac tataaaagaa aacctgcttt ttgtaaactc ttcggcaact
     3901 aatgatgaac taaatgatgt attggaaaaa gcaagttgcc aaaatttatt agaacgtgca
     3961 gataatggca ttaatactgt aattggagaa ggaggtttaa aactaagtgg tggcgaaaag
     4021 caaagaattt ccattgccag agccctttta agaaaacctc atttattaat ttttgatgaa
     4081 gcaacaagtg ctttagatag tataacagaa gaggaaatta ctaatactat taagcaaatt
     4141 agccttcata aaaagcaagt aaccataatg atagcacata gattgagtac tattatgcat
     4201 gcagatagaa tatttgtttt agaaaaaggt aaaatagttg aaacaggtaa tcaccaagaa
     4261 ttactaaatg aaaaaggttt atattttgct atgtggaggc aacaaattgg agaaagaaaa
     4321 taaaaaaaat tctttaaaaa tgcaaccaat taaactctac accctttttg ctaaaaccaa
     4381 tactaggtgc tggaaattta aatgaattta aaaaaaacaa agccactttt aaaaacttac
     4441 tttttgtttt aattttagtt aaatttatat ctggtgcaat ataccattgt ctatatctag
     4501 gtatatcact tctatcaatt acattattgt tttcatcttt ccaaatattg tcatctccat
     4561 caaacattcc atctgcaccg taaccaacag caacatttaa ccaagagggt acattgcttt
     4621 taggaaaaaa agattttata ttagcactta accaataagt ttggccattg tagtctttca
     4681 acattctgtt taaaaaactt ttgccaaaaa gcttatcagc tcggttatgt aaaaacgggt
     4741 ctttataatt tactttatga aacgaaaatt ttaaatcaac tctttgttca tcccataata
     4801 attcttgtgc aataagcagt ccactaccat aacaatttgc aataaaatct ccccagctcc
     4861 aaccccaacc aacactataa gcatctaaaa attcaatagc agtttggtat gttgcaccac
     4921 tcaatcctcc taaccaaatt ctttgtttac gtggtaagcc agcccaacgc caaagttcca
     4981 tacttgcttt actctctaca taagcactat atgcatggcc tgctttatct acctgtaacc
     5041 actcttcatt atcattaaaa aaatgaaaat tagattgtgg ataattttta taccaagcat
     5101 tatacaatcc aatcatagca cccccatatc ctacaatatt cactgcagaa accaacctaa
     5161 ttcttttata atttcttaaa tgagatggag tattttgaga aagcgaatca aaaaaatttt
     5221 gttctgtaag caaattaaaa cgataaggtg cgtacatttt aattttatct acagtagcaa
     5281 cctgtgcatt gctattttgg taaataccca acccaaataa tatgaagaaa caaaatttaa
     5341 aataaaattt tgtttccact tttttattat aaagcttaag tactttcatc caaaaactat
     5401 gtataaaaaa gtcgttaaat atgcttaaaa ttgcactcat taatgtacag gttatcaaca
     5461 aatgtatttt attattattt agatttttaa aaataaaatt aattaacaat tatggataat
     5521 tcaatttcag ttaaagttaa cgaatggctc ggtggaaatt atgatgaaac aaccaaacaa
     5581 attattaaaa atttacaagc agaaaatgaa ctagaactta ctgagagttt ttataagaac
     5641 ttagaatttg gtactggtgg cctaagaggc ataatgggtg ttggcactaa cagaatgaat
     5701 aaatatactg ttggtatggc tacgcaaggc tttgcaaact atttaaaaaa agtatatggg
     5761 caagaagaaa taaaaattgt cattgggcat gatagtagaa ataatagt
//
LOCUS       DAOQHP010000107         5771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7688_length_5771_cov_3.556683, whole genome shotgun sequence.
ACCESSION   DAOQHP010000107 DAOQHP010000000
VERSION     DAOQHP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7688_length_5771_cov_3.556683"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="paaC"
                     /locus_tag="PLY81_04730"
     CDS             <1..518
                     /gene="paaC"
                     /locus_tag="PLY81_04730"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNF29562.1"
                     /translation="IESEYKNLLLVELPKEDWAHTVLRQFYFSVFLFYLYQQLQQNKD
                     VQIAAIATKAIKEVAYHVRWSGEWVIRLGDGTEESNQRMQYAVEAYKNFTGEFFVLTN
                     YEKQMQIDFELIQQQWNDKVAAVFNDATLSFTNSNFHQVGGKEGRHTEYLGYILTDMQ
                     YLQRTYPNATW"
     gene            512..1015
                     /gene="paaJ"
                     /locus_tag="PLY81_04735"
     CDS             512..1015
                     /gene="paaJ"
                     /locus_tag="PLY81_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNF29563.1"
                     /translation="MVNEQSISVANIWKIVETIPDPEIPVISIVELGVVREVKIENDI
                     IEIIITPTYIGCPAMDMITMHIKLALMEAGFKNVKVTSVLSPAWTTDWMTEETKNKLK
                     AYGIAPPNTKQIVCTPDLFIQDEAVACPHCNNFNTKMVSRFGSTACKALYFCNNCNEP
                     FDYFKCH"
     gene            1054..1830
                     /locus_tag="PLY81_04740"
     CDS             1054..1830
                     /locus_tag="PLY81_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF29564.1"
                     /translation="MPTVLFEIKNNIGFITLNRPEKFNAFNREMALLLQDILTTCNNN
                     NQVRAVFITGAGKAFCSGQDLAEVVDPNGPGMNRILAEHYNPIIQKIRSLEKPVIAAV
                     NGVAAGAGANIALCCDIVIAAENATFIQAFSKIGLIPDSGGTYFLPRLIGWQKASAIM
                     FLGDKITAVEADKWGMIYKFFSADVFETEALKIANTLAEMPTKGLYFTKQALSNSFTN
                     TLHEQLVDEDKLQQQAAATFDFKEGVAAFLEKRKPIFKGE"
     gene            1880..2296
                     /gene="paaI"
                     /locus_tag="PLY81_04745"
     CDS             1880..2296
                     /gene="paaI"
                     /locus_tag="PLY81_04745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNF29565.1"
                     /translation="MTTTPQQVVAHMMQHDLFSQWLGIEVLEIKEGYSKIKMLVRPEM
                     ANGFNIVHGGIAFSLADSAFAFACNNRNNLSVALDTSINFTKPVHVGDILIAEAKELH
                     NGKSTGLYHITITNQNNHIVAMFKGTCFRTSKPLVK"
     gene            complement(2302..2664)
                     /locus_tag="PLY81_04750"
     CDS             complement(2302..2664)
                     /locus_tag="PLY81_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNF29566.1"
                     /translation="MKKKTLVLGASDNTERYSNMAVKKLVAHGHTVVAVGNKDTFIDT
                     IPVYKEKIIEDGIDTVTLYLSPKNQIPFYSYIISLKPKRIIFNPGTENEDLVTLAKQN
                     NIQTIEACTLVMLNTGQY"
     gene            complement(2666..3112)
                     /locus_tag="PLY81_04755"
     CDS             complement(2666..3112)
                     /locus_tag="PLY81_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF29567.1"
                     /translation="MSIITAERIAADFEMQMTDATGNTMKMDIPIEQGGNGKGLRPMQ
                     TMLAALCGCSTVDIVSILNKQKQTIETFKVFVDGEREKNKTMALWQTIHLVFEMSGNI
                     EPDKAFRAVQLSLDKYCSVAETLRKAGASISFTVILNNEKLNYQPE"
     gene            3217..4632
                     /locus_tag="PLY81_04760"
     CDS             3217..4632
                     /locus_tag="PLY81_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF29568.1"
                     /translation="MLIKQLYTGCLSEAAYFIANNGEAAVIDPLRDIDEYLQLAKEHK
                     VSIKYIFETHFHADFVSGHLDLAAATGATIVYGPNTDTKLKVHIAFDGEVFKIGNLSV
                     KVLHTPGHTLESTCFLLKDEQEKDYAVFTGDTLFVGDVGRPDLAQKGADLTMNDLAGM
                     LYESLQNKIFPLDNQVIVYPAHGAGSSCGKNLGKETYSTIGEQKISNYALQTQTKEDF
                     VKAVTEGLLAPPQYFPINAKINKEGYQSISTILENGLKALSVNEFKAKASDENVIIVD
                     TRNATVFTNGFVPNSISIGLEGRFAEWAGSILPFTKSILLVTEKGKEKESVVRLSRVG
                     FDKFAGYLDGGFEAWQLANENTDMIVDVECDELAMDIPFDENLIVVDVRRETEYADGH
                     LKQSINIPLNELTDPASMANLEDNHNIYIHCAGGYRSVIAASLLKQQGIHNLRNVLGG
                     WSKIKELADKFEIEKSTEVLN"
     gene            complement(4869..5639)
                     /locus_tag="PLY81_04765"
     CDS             complement(4869..5639)
                     /locus_tag="PLY81_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNF29569.1"
                     /translation="MIIDTHCHLYLDEFEKDRLEVMQRAIKKGVQKFYLPSIDSTVID
                     KMLRMEKEYPKNCFLMMGLHPCSVNENIDAELTTVLNCLKQRPFAAVGEIGLDFYWDT
                     SFKEQQYNAFKTQMQWAIDYNLPIVIHSRNAMQETINTVKPFAAMGLRGIFHCFSGSI
                     EEANTIINMNFLLGIGGVITYKKSGLAETLKEIALSNVVVETDAPYLTPLPFRGKRNE
                     SAYLEYIISEIAKVKNCSVQEVITTTTNNAENLFNKYE"
BASE COUNT         1990 a          849 c          966 g         1966 t
ORIGIN      
        1 ggatagaaag tgaatacaag aatttgttat tggtagaatt acctaaagaa gattgggcac
       61 atacagtatt aaggcaattt tatttttctg tttttctgtt ttatttatac caacaactgc
      121 aacaaaataa agatgtacaa attgcagcta ttgccacaaa ggcaataaaa gaagttgctt
      181 accatgttcg ttggagtggg gaatgggtaa tacgtttagg agatggcaca gaagaaagca
      241 accaacgtat gcagtatgct gtggaggctt acaaaaactt tacaggagaa ttttttgtat
      301 taaccaatta tgaaaaacaa atgcaaatag attttgaatt aatacaacaa cagtggaacg
      361 ataaagttgc agcagttttt aatgatgcaa cactttcttt taccaatagt aattttcatc
      421 aggtaggagg taaagaagga aggcatacag aatacttagg atatatttta accgatatgc
      481 aatatttgca aagaacctat cccaatgcta catggtaaat gaacaatcca tatcagtagc
      541 aaatatttgg aagatagtag aaactattcc cgacccagaa atacccgtaa tttcaattgt
      601 agaactaggc gttgtaagag aggtaaaaat agaaaatgat attattgaaa ttataattac
      661 accaacttat ataggctgcc ctgcaatgga tatgataacg atgcatatta aactagcatt
      721 aatggaagca gggtttaaaa atgtaaaagt aacttctgtt ttatcaccag catggaccac
      781 agattggatg acggaagaaa caaaaaacaa actaaaagcc tatggtattg ctccacccaa
      841 tactaaacaa atagtttgta cacccgattt gtttatacaa gatgaggctg tagcctgtcc
      901 acattgcaat aactttaata ctaaaatggt atctaggttt ggcagtacag cctgtaaagc
      961 tttatatttt tgcaacaatt gtaatgagcc tttcgattat tttaaatgtc attaaaaata
     1021 ataattacat ctactttacc ataaataata tatatgccca ctgttttatt tgaaataaaa
     1081 aataatattg gttttataac cttaaaccgc ccagagaaat ttaatgcttt taacagagaa
     1141 atggctttgt tattacaaga tattttaacc acttgcaata acaataacca agtaagagca
     1201 gtttttataa ccggtgcagg aaaagcattt tgctctggtc aggatttggc agaagtagtg
     1261 gaccctaatg ggcctggtat gaacagaatt ttagcagaac attataaccc cattattcaa
     1321 aaaataagaa gcctggaaaa gcctgtaata gctgcagtaa atggtgttgc agcaggtgct
     1381 ggtgcaaata ttgctttatg ttgcgatatt gtaatagctg cagaaaatgc aacattcatt
     1441 caggcatttt ctaaaatagg attgatacct gacagtggtg gaacttattt tttacctcgc
     1501 ctaataggct ggcaaaaagc tagtgcaata atgtttttag gagataaaat tacagcagtg
     1561 gaagccgaca aatggggtat gatttataaa tttttttctg ccgatgtttt tgaaacagaa
     1621 gccttaaaaa ttgcaaacac tttagccgaa atgccaacca aaggtttgta ttttactaaa
     1681 caagcattga gtaattcttt tacaaatact ttacacgagc aattggttga tgaagataaa
     1741 ttgcaacaac aggctgctgc aacctttgat tttaaggaag gtgttgcagc ttttttagaa
     1801 aaaagaaaac caatttttaa aggagaataa tgaagtgtaa gaaagtatat actttattat
     1861 tctttaaata atttaataca tgaccaccac accacaacaa gttgttgcac acatgatgca
     1921 gcatgattta ttttctcaat ggttaggaat agaagtttta gaaataaaag aaggatacag
     1981 caaaataaaa atgttggtta gacctgaaat ggcaaatggc tttaatattg tacatggtgg
     2041 aattgccttt agtttggcag atagtgcatt tgcctttgca tgtaataata gaaacaatct
     2101 ttctgtggca ttagatacct ctattaattt tacaaaacca gtacatgttg gcgatatatt
     2161 aattgcagaa gcaaaagaat tgcacaatgg aaaaagtaca ggtctatacc atattacaat
     2221 taccaatcaa aacaaccata ttgtagcaat gtttaaaggc acttgtttta gaacaagtaa
     2281 acctttggtg aaataacttt atcaatattg ccctgtatta agcattacta aagtacaagc
     2341 ttctatagtt tgaatattat tttgtttggc tagtgtaact aaatcttcgt tctctgtacc
     2401 agggttaaaa ataattcttt taggttttag agatataatg taagaataaa aaggtatttg
     2461 gtttttaggg cttaaataaa gagttactgt gtctattcca tcttcaatta ttttttcttt
     2521 atatactggt atagtatcaa taaaagtgtc tttgttacca acagcaacaa cagtatgtcc
     2581 atgagcaact aactttttta ctgccatatt gctgtatctt tctgtattat cagaagcacc
     2641 taaaactaaa gttttcttct tcattttatt ctggttggta atttaatttt tcattattta
     2701 atataaccgt aaagctaatg ctggcaccag cttttcttaa agtttctgca accgagcaat
     2761 atttatctaa cgataattga acagctctaa atgctttatc tggttcaata ttgccactca
     2821 tttcaaaaac taaatgaatg gtttgccaca aagccatggt tttatttttt tccctttcgc
     2881 catctacaaa aactttaaat gtttcaatag tttgtttttg cttatttaat atacttacaa
     2941 tatctacagt gctacaccca caaagtgctg caagcatagt ttgcatgggt cttaatcctt
     3001 ttccatttcc accttgttca atgggtatat ccattttcat agtgttacct gttgcatcgg
     3061 tcatttgcat ttcaaaatct gcagcaattc tttcggcagt tataattgac ataaaatata
     3121 atttttactt ctaagttaag tgttttttaa attacaaata tggtgtaaga atgtagaata
     3181 tatttttata tggtatcttt gaaaaaatta ttatagatgc taattaaaca attatataca
     3241 ggctgtttaa gcgaagctgc ttattttatt gcaaataatg gagaagcagc agtgatagac
     3301 cccttaagag atattgatga atatttacaa ttagccaagg aacacaaggt tagtattaaa
     3361 tatatttttg aaacccattt tcatgctgat tttgtaagtg ggcatttaga ccttgcagca
     3421 gcaacaggtg caaccattgt atatggccct aataccgata ctaaactgaa agtgcatatt
     3481 gcttttgacg gagaggtttt taaaattggt aacctatctg taaaagtatt acatactcct
     3541 ggtcacactt tagaaagtac ttgcttttta ttaaaagatg aacaggaaaa agattatgca
     3601 gtttttacag gtgatacact ttttgtaggc gatgtaggaa ggccagattt agctcaaaaa
     3661 ggggcagatt taactatgaa tgatttggca ggtatgttgt acgagagctt acaaaataaa
     3721 atttttccct tagataatca ggtaattgtt tatcctgctc atggtgcagg aagcagttgt
     3781 ggtaaaaatt taggtaaaga aacatatagt actattggag agcaaaaaat ttctaattat
     3841 gctctacaaa ctcaaacaaa agaagatttt gtgaaagcag taaccgaagg gttgcttgct
     3901 ccacctcaat attttcctat taatgctaaa ataaataaag aaggctacca gagtataagt
     3961 acaattttag aaaatggatt aaaagcttta tcagtaaatg aatttaaagc taaagcaagt
     4021 gatgaaaatg taattattgt agatactcgt aatgcaacag tttttaccaa tggttttgtg
     4081 cccaatagta tttctatagg gttagaaggc agatttgcag aatgggctgg tagtatttta
     4141 ccttttacta aatctatttt attagttaca gaaaaaggga aggaaaaaga aagtgttgta
     4201 agattatcga gagtcggatt tgataaattt gcaggctatt tagatggtgg gtttgaagca
     4261 tggcaacttg ccaacgaaaa tacagatatg attgtagatg ttgagtgtga tgaattggca
     4321 atggacattc cttttgatga aaatttaatt gtagtagatg ttaggagaga gacagaatat
     4381 gctgatggtc atttaaagca atctattaat attcctttaa atgaacttac cgatcctgca
     4441 agtatggcta atttagaaga taaccataat atttatattc attgtgcagg tggttacaga
     4501 agtgtaattg cagcctcttt attaaagcaa caaggaattc ataatttaag aaatgtttta
     4561 ggagggtgga gtaaaataaa agagttagca gataaatttg aaattgaaaa aagtacagaa
     4621 gtgttaaact aatttttatg gaacctgaat taagaaaaat gctaaataga attgtttata
     4681 cactttctac cttaattgtt tggttacttt ctaatattac atttggtatt aaatttcagt
     4741 ttgctttttt taagaatcat ttttcattag gtaatatttt attttatata tggcttgttg
     4801 ttagtactgt tttgatgctg tttttaataa aaaaaatttg gagtaaacct ttaaacataa
     4861 atctataact attcatactt gttaaacaaa ttttctgcat tgtttgtggt ggtggttata
     4921 acctcttgta ctgagcagtt ttttactttt gcaatttcag aaattatata ctctaaatag
     4981 gcactttcat tcctctttcc cctaaagggt aatggagtta aataaggtgc atctgtctct
     5041 accacaacat tactcaatgc tatttctttt aaagtttctg ctaaaccaga ttttttatag
     5101 gttattaccc ctccaatacc taataaaaaa ttcatattta taattgtatt agcctcctca
     5161 atgcttccag aaaaacaatg aaatattcct ctcaacccca tagcagcaaa aggctttaca
     5221 gtgttaatgg tttcttgcat ggcatttcta ctatgtatta caattggcaa attataatct
     5281 atagcccatt gcatttgagt tttaaatgca ttatactgct gctctttaaa acttgtatcc
     5341 caataaaagt ctaaaccaat ttcaccaact gctgcaaatg gtctctgttt taaacagttt
     5401 aaaacagttg ttaattcggc atctatattt tcgtttaccg agcaaggatg taagcccatc
     5461 attaaaaaac aatttttagg gtactctttt tccatcctca gcattttatc tattactgta
     5521 ctatctatgc ttggtaaata aaatttttgt accccttttt ttatagccct ttgcataacc
     5581 tctaacctgt ctttttcaaa ttcatctaaa tataaatggc agtgggtatc aattatcatg
     5641 tatttttttt taagttatat actaaaattt atactaattc gtttttatag agtaattact
     5701 tttttctacc aatataagta attataaaaa ccaattttgt ttaaattaaa atccaatgtt
     5761 gccaatgtac a
//
LOCUS       DAOQHP010000108         5755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7711_length_5755_cov_4.733684, whole genome shotgun sequence.
ACCESSION   DAOQHP010000108 DAOQHP010000000
VERSION     DAOQHP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_5755_cov_4.733684"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..1547
                     /gene="pdhA"
                     /locus_tag="PLY81_04770"
     CDS             552..1547
                     /gene="pdhA"
                     /locus_tag="PLY81_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNF29570.1"
                     /translation="MATKFSKETYLYWYELMQLIRQFELKAEEMYKMAGKIRGFFHAY
                     VGQEAIAAGCMTATRHEDPYITAYRDHGFALAKGTSANACMAELYGKATGCAKGKGGS
                     MHFFDVENYFFGGHGIVGAQIGTGAGLAFAEKYKGTDNVVLCFFGDGASRQGMLHETF
                     NMAMNWKLPVIFICENNHYAMGTSIERTSNVIDIYKLADAYEMPADQIDGMSPEAVHT
                     GIARAVKRAREGGGPTLLEMKTYRYKGHSISDPQKYRTKEEVEEYKEKDPIIQVANTI
                     LENNFANQEDLNNIDAKIAAIVEKSVQFAEESPWPDDSEVLKDVYADPNYPFIVD"
     gene            1590..2276
                     /locus_tag="PLY81_04775"
     CDS             1590..2276
                     /locus_tag="PLY81_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29571.1"
                     /translation="MSEQIVKKDMWMQLESFWNKKKKIILTVGGAIVVIAGGLYGYKQ
                     YIVLPKEEKAADAIYKVQQFFQQDSSNLVINGNNETGAKGALYIIKNYSGTKAANLAY
                     YYAGISYLKLKDFNNAIKYLKDFSTNALQVQMSAYTALGHAYAESGKKDEAVSYYKKA
                     ATTFEEDKDNSAENLFLAAQLLEIMNKPKEALDIYKTIKQKYPQTSRGYEADKFIYRL
                     SIEKNDFSIN"
     gene            2279..2764
                     /gene="ribH"
                     /locus_tag="PLY81_04780"
     CDS             2279..2764
                     /gene="ribH"
                     /locus_tag="PLY81_04780"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNF29572.1"
                     /translation="MATKGNKNLNKGIPKLKDAFIVIIKTEWNSKIVNQLENGVIAVL
                     KENNINYKTIIVPGAIEIPFALKQHYTYSSTLPSAYITLGTVIKGDTPHFDYVCKFVT
                     EGILQLNLSLDVPVVFGVLTVNTEKEATERTTGSHGNKGEEFAITAIKMIALNNTLKK
                     L"
     gene            complement(2835..4922)
                     /locus_tag="PLY81_04785"
     CDS             complement(2835..4922)
                     /locus_tag="PLY81_04785"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNF29573.1"
                     /translation="MKNKHFNWLVLFFLTITTITIIAACNKKFDEPDTTFVDPNLPIT
                     MTLKELKALLPTTGTVVEITVDKTISGIVVADDKSGNFYKSISIQDATGGVSLRLDGT
                     NLYTSYPVGRRVYINVKGLCVGEYGGMIQLGAVNKATSPISQTPIPQNLFDKYVIKGG
                     FGNVITPKIFSSITDLKTTLQDTVQNTLIQLENFQFSAADTLKTFADPSLASSAVNLN
                     IQNCSGNTITLRTSSYSNFAGIQVPKKKGTITAIYTFFNGTKQLLIRDTSDVQFKDDR
                     CGSSISPTLVYKSIQDVRAMGDGAIMPANTGIRGTIVSNTLNESNGNYRIQDGSGYGL
                     QLRFPSINPNFALNDSVAVDISGLSLSLFNGDLQITNVVNAIKLATGTVVPRNTTVSQ
                     INSNINSWSSTVVKLTNVSIAETSSNATGKNYTVTDASGSIVSFVRTTLGVSSLPANA
                     TTFIGYVSLYNGTPQITIRNAADVVAGGSGPGGGGSTTGITLGSTSPFTLNFNGIGSG
                     LPTGVSVYTGATTTSFGTAGSFNSALGLWANFSAGFKNFASATGLSATSDQTAQDAAS
                     NRALGVRQTSSTGYDPGASFVFLLDNTTGKNNVQLQFLLQSLDNSVGRTVTWTVDYAL
                     GDSPASFTAASASGTLTTSNVFSSNTINVNFGNAIDNKSQKVWIRVSALSNSTGSANR
                     PSTAIDDVSISWN"
     gene            complement(4974..>5755)
                     /locus_tag="PLY81_04790"
     CDS             complement(4974..>5755)
                     /locus_tag="PLY81_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29574.1"
                     /translation="AGYVLNAPKIKVRLTGYFTKIDNQMNGMTFYHDDYRNFVNYAMS
                     NIDKLHFGLEFGAEAKIMRNVTLNVAVAAGRYYYNSRQDATVTVDNDATVLTKDLIYS
                     QNYRLAGTPQNTFSLGFSYRSPKFWFVSLSANYAANRWLDFNPIRRTFDAVEGLDITK
                     PNEAAIRESILAQTKLEDDYTIDFFGGWSWKLPKSFGFKKSTYLVFNLGINNLLNNKN
                     IISGGFEQLRFDYADKNINKFPPKLYYAYGLNYFGSVTLRF"
BASE COUNT         2066 a          885 c          919 g         1885 t
ORIGIN      
        1 attttatgta aaaaagataa aataaaacaa taaaatgctg cttaaaaata aaaatctggc
       61 actgttatct tcaaagtcgt aatacgctat aggtagttgt gcgtttgcaa caaaacccat
      121 aaaaattaat aaagataaaa gtgtaaattt tgaaactctc atatatgtat tatttaaaaa
      181 aaatatttta tgaatattag aaccgacaaa aatagcaaaa tatagtacaa agaaaacgtt
      241 ttttgcgtaa aatattgaat tattttcaaa aatattaaac accaaaagca cttctatttt
      301 aaagttttaa gttaaaataa aattttatgt aaaaaagata aaataaaaca ataaaatgct
      361 gcttaaaaat aaaaattggt taactctatc cgtgtttgac tataattagt ctttacagtt
      421 taaccatgat acatttttac tttcaatatt attatctaac aagaaattag attatttaga
      481 taaatcaata ctatattttt aattaagtaa tgcttaaacc ctatatttgc caaccaaaat
      541 tttttgtact agtggcaact aaattttcaa aagaaactta tctatattgg tacgaactca
      601 tgcaacttat tcgtcagttt gagttaaagg ctgaagaaat gtataaaatg gctggaaaaa
      661 tacgtggttt ttttcatgcc tacgttggcc aagaagcaat tgctgcaggt tgtatgacag
      721 ccaccagaca cgaagaccct tatattacag catatagaga tcatggcttt gcacttgcaa
      781 aaggaacatc ggcaaatgca tgcatggccg aattgtatgg taaagcaact ggttgtgcaa
      841 aagggaaagg cggtagtatg cacttttttg atgttgaaaa ttattttttt ggtggacacg
      901 gaattgtagg tgcacaaatt ggaactggag ctggattagc atttgcagaa aaatataaag
      961 gtactgataa tgtagtgctt tgtttttttg gggatggagc atctaggcaa ggcatgcttc
     1021 acgaaacgtt taatatggca atgaattgga agttgcctgt aatttttatt tgcgaaaata
     1081 accattatgc aatgggtact tctattgaac gtactagtaa tgtaattgat atttataaat
     1141 tagccgatgc ttatgaaatg cctgcagacc aaattgatgg catgagcccg gaagctgtac
     1201 atactggtat tgcaagagct gtaaaacgtg caagagaagg tggtggacca actttgcttg
     1261 agatgaaaac ttatcgctac aaaggtcatt caattagcga ccctcagaaa tatagaacta
     1321 aagaagaagt agaagaatac aaagagaaag atccaatcat tcaagtagct aataccattt
     1381 tagaaaacaa ctttgcaaat caagaagact taaacaatat tgatgcaaaa attgctgcca
     1441 tagttgaaaa aagcgtacaa tttgcagaag aaagcccttg gccagatgat agtgaagtat
     1501 taaaagatgt atatgctgac cccaactacc catttatagt agattaaggt aaactaaaca
     1561 atcaattaaa aaacaattat ttatatttta tgagtgaaca aattgtaaag aaagatatgt
     1621 ggatgcaatt agaaagtttt tggaataaaa agaaaaaaat tattcttact gttggtggtg
     1681 caattgttgt aattgctggc ggattatatg gttacaaaca atatattgtt ttacctaaag
     1741 aagaaaaggc tgctgatgct atttataaag tacaacaatt ctttcagcaa gactcttcca
     1801 accttgtaat aaatggtaat aacgaaactg gagcaaaagg tgctttatat attattaaaa
     1861 actatagtgg cactaaagca gctaatttag cttattacta tgcaggtatt agttatttaa
     1921 aactgaaaga ttttaacaat gctattaaat atttaaaaga ttttagcact aatgctcttc
     1981 aagtacaaat gagtgcatat acagctttag gccatgctta tgcagaatct ggcaaaaaag
     2041 atgaagcagt gagttattat aaaaaagctg ctacaacttt tgaagaagac aaagacaact
     2101 ctgccgaaaa tttattttta gcagcacagt tattagaaat aatgaataag ccaaaagagg
     2161 cattagatat ttataaaact atcaaacaaa aatatcctca aacaagccgt ggatatgaag
     2221 ctgataaatt tatttacaga ttaagtatag agaaaaacga ttttagcata aactaattat
     2281 ggcaaccaaa ggcaataaaa acttaaataa aggcatccca aaattaaagg atgcctttat
     2341 tgtaattatt aaaacagaat ggaatagtaa aattgtgaat caattagaaa atggggttat
     2401 tgctgtttta aaagaaaata atataaacta taaaacaatt atcgttcctg gtgctattga
     2461 aataccattt gcattaaaac aacattatac ttatagttct actttaccta gtgcatatat
     2521 tactttaggc actgtaatta aaggagatac tcctcatttt gactatgtat gcaaatttgt
     2581 aacagaagga attttacaat taaacctaag tttagatgtt cctgttgtat ttggcgttct
     2641 aacagtaaat acagaaaaag aagctaccga aagaactaca ggtagccatg gaaataaagg
     2701 agaggaattt gctataactg caattaaaat gattgcttta aacaatactt tgaagaaatt
     2761 ataatgattt taattccttc accacataaa aaagccttgt aatactacaa ggcttttttg
     2821 taaaccatct ataattaatt ccagcttata gatacatcat ctatagcggt ggaaggtctg
     2881 tttgcactac cagttgaatt tgaaagtgca gaaactctta tccaaacttt ttgagattta
     2941 ttgtcaatag catttccaaa atttacatta attgtgttac tagaaaatac gttacttgtg
     3001 gttaatgtac ctgaagctga agcagcagta aatgaagctg ggctatcccc taaagcataa
     3061 tccacagtcc aggttacagt tcttccaacc gaattgtcta aagattgtaa taagaattgt
     3121 aattgaacat tatttttacc cgttgtatta tctaataaaa atacaaaaga agctccagga
     3181 tcataacctg tagaactagt ttggcgaaca cctaaagctc tattgcttgc agcatcttgt
     3241 gcagtttgat ctgaagttgc agataaacca gttgcagaag caaagttttt aaatcctgca
     3301 ctaaaattag cccaaagtcc caaagcagaa ttgaaagaac cagcagtgcc aaaagatgtt
     3361 gttgtagccc ctgtataaac acttacacct gttggtaaac cagagccaat gccattaaag
     3421 tttaatgtaa atggtgatgt agaacccaaa gtaatacctg tagtgctacc acctcctcca
     3481 ggaccagaac caccagcaac aacatctgct gcatttctaa tggttatttg aggcgttcca
     3541 ttatataaag aaacatatcc tataaaagta gttgcatttg ctggtaatga tgaaacacct
     3601 aaagttgttc taacaaatga aacaattgag ccagatgcat cggttactgt ataatttttt
     3661 ccagttgcat ttgatgaggt ttcagctatg gaaacattag ttagtttaac aactgtagaa
     3721 gaccatgaat tgatgtttga gttgatttga gagacggttg tatttctagg cacaacagta
     3781 ccagttgcta attttattgc attaaccaca ttggtaattt gtaaatctcc attgaataaa
     3841 gaaagtgata agccagaaat atctacagca acactatcat ttaatgcaaa gtttggatta
     3901 atagatggga aacgtaattg taacccataa ccactaccat cttgaatacg gtagttacca
     3961 tttgattcgt ttaaagtatt agatacaata gtacctctaa tgcctgtatt tgctggcatt
     4021 atggccccat ctcccattgc tctaacatct tgaatagatt tataaactaa agttgggctt
     4081 atactgctac cacatctgtc atctttaaat tgtacatcgc tggtatctct aattaataat
     4141 tgtttagtac cattaaaaaa agtatagata gcggttatag ttcctttctt tttaggtact
     4201 tgaattccag caaaattaga atagctactt gttcttaaag ttatagtatt acctgaacaa
     4261 ttttgaatat ttagatttac agctgaagat gctaaactag ggtctgcaaa agtttttaaa
     4321 gtatctgcag cactaaattg aaagttctct aactgtatta aagtattttg aacagtatct
     4381 tgtaatgttg tttttaaatc ggttatgcta gagaaaattt taggagtaat tacatttcca
     4441 aatcctcctt taatcacata tttatcaaac aaattttgtg gtattggtgt ttgagaaatt
     4501 ggtgaagtag ctttgttaac tgcacctaat tgaatcattc caccatactc acccacacac
     4561 aatcctttaa cattaatata tactcttctt cctacagggt agcttgtata taaattagtt
     4621 ccatctaatc ttaaactaac gccaccagtt gcatcttgta tagagattga tttataaaaa
     4681 tttccgcttt tatcatctgc aactactatt cctgaaatag ttttatctac tgtaatttct
     4741 acaactgttc ctgttgtagg taacaatgct tttagctctt ttaaagtcat tgttattggt
     4801 aagttggggt caacaaaagt tgtatctggt tcgtcaaatt ttttattaca tgctgcaata
     4861 atagtaattg tggttatcgt taaaaaaaac aatactaacc aattgaagtg tttatttttc
     4921 atatttctca tttgtaagag ttataataat tttttactga attttttaat ttattaaaat
     4981 ctaagtgtta cactgccaaa ataatttaag ccataagcat aataaagctt aggtggaaac
     5041 ttattaatat ttttatcggc ataatcaaat ctcaactgct caaagccgcc agagatgata
     5101 tttttattgt ttaataaatt atttatacct aggttaaata ccaaataggt tgatttttta
     5161 aacccaaagc tctttggtaa tttccaactc caaccaccaa aaaagtctat agtataatcg
     5221 tcttctaatt ttgtttgtgc taaaatactt tctctaatag ctgcctcgtt tggcttggta
     5281 atatctaatc cttccactgc atcaaaagtt cttctaatcg ggttaaaatc taaccatcta
     5341 ttagctgcat agttggcact taaactaaca aaccaaaatt tagggcttct ataagaaaat
     5401 cctaagctga atgtgttttg aggggtgcct gctaatctat agttttgaga ataaattaaa
     5461 tctttagtta gaacagtagc atcattatct actgttacag tagcatcttg acggctgtta
     5521 taataatatc ttcctgcggc aactgctaca tttaatgtta catttctcat tattttagct
     5581 tctgcaccaa actctaaccc aaaatgtagt ttatctatgt tactcatagc atagtttaca
     5641 aaatttctgt aatcatcatg ataaaaagtc attccattca tttggttatc tattttagta
     5701 aaataacctg taagtctaac ttttatttta ggtgcattta acacataacc tgctt
//
LOCUS       DAOQHP010000109         5686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7856_length_5686_cov_3.876931, whole genome shotgun sequence.
ACCESSION   DAOQHP010000109 DAOQHP010000000
VERSION     DAOQHP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_5686_cov_3.876931"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PLY81_04795"
     CDS             <1..1407
                     /locus_tag="PLY81_04795"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNF29575.1"
                     /translation="ANVFAANGCKVFLFEALRPTPELSFAIRHLGCKAGVVCTASHNP
                     KEYNGYKAYWNDGSQLVPPHDKNVIKEVEAIANVDEVKWTGGQANITLLGKEMDEAYI
                     GMVKALSIYPDVIAKQHNLKIVYTPIHGTGITIVPETLKQFGFSNVTIVEEQATPDGN
                     FPTVVYPNPEETEAMSIGLKKAIEIDADILCGTDPDSDRVGIGVKNHKGEWVLMNGNQ
                     TLVLAFAYLIEARKLKGLAKPNDMVISTIVSTDMAHNVAQQNNISSYGVLTGFKWIAE
                     LIREKEKDENYIIGGEESFGLMIGNGTRDKDAVSAVALLCEMAAYEKEHGRSLFDKLI
                     ELYIKYGFYYESLISITKKGMNGQAEIAEMMEGYRTNPPKVINGSKVVEILDYEKQIG
                     KDFESGDSWKIELPKSNVLQFITEDGSKISARPSGTEPKIKFYFSVNTKLNSKNEFDE
                     KFELLQNKIKGIIASLNL"
     gene            complement(1479..1928)
                     /locus_tag="PLY81_04800"
     CDS             complement(1479..1928)
                     /locus_tag="PLY81_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF29576.1"
                     /translation="MQQAKKGDTVKIHYHGRLTDGTTFDSSEGREPLQFELGSGMVIP
                     GFDNGVTGMSVGEKKTINIPAAEAYGESNPDMIMDFPKAQFPPDLTPEVGMQLTMHNG
                     AGQQFNVTIIELKEESVILDANHHLAGKELIFDLELVDIKAKSLIIT"
     gene            2229..2453
                     /locus_tag="PLY81_04805"
     CDS             2229..2453
                     /locus_tag="PLY81_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNF29577.1"
                     /translation="MFWTLELASYLEEAPWPATKDELIDYSIRSGAPIEVVENLQELD
                     DEGTEVYESIEDIWPDYPSQDDFMFNEDEY"
     gene            complement(2481..4001)
                     /locus_tag="PLY81_04810"
     CDS             complement(2481..4001)
                     /locus_tag="PLY81_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNF29578.1"
                     /translation="MQPIATSFLLQSLGYTLINSLWQVAIIWLLYILFTNFLKLPSKH
                     KYLIAVVAQIIGFCSFIYTFCYNFFSTSSNLKNGLFLQSNNFNSFFIPLILKVEKALP
                     FLSVVYLLVTIILLVKVIIGFNHTNNLRINKLHKVNAEWRVFVNEMAIALGLNKEVKI
                     YLSELVNTPLTIGYFKPIILLPLASINNLSTYQIEAVILHELAHIKRFDFLVNILLLI
                     VEIVLFFNPFSRLLSEHIKQERENSCDDWVSQFKYCPNLYANALLQIAMSNLRPYQFG
                     MAAISNKNELLKRVNRITNSKSAKNNYSALRFGFVFMLFALLSFTFFFSGNIQNESIN
                     KATNRLLSNTIKNEVKTLSHTGLAQNKSSNNQQEQYILFEKLVKEPADIESISTNDNK
                     RKLSTNSEVGKPFNSKKNSPKNSKPKINDTELEEEDFEIPESSMPFSYAFNTLNDSSY
                     TVTATDIEQENNISKLIIRKKGLTEKELEKIIPKRYDIVISNEGKPDKKIILEVWQ"
     gene            complement(4010..4393)
                     /locus_tag="PLY81_04815"
     CDS             complement(4010..4393)
                     /locus_tag="PLY81_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNF29579.1"
                     /translation="MFNKQKIKPTESELEILRILWDKKSASVREVHEILSLHKESGYT
                     TTLKLLQIMFEKSLVIRDDTSKVHIYTPNVSKESTQKQFMHKMVETLFGGSSVQLAMQ
                     ALGNSKPSKDELEEIEKILNRLKNK"
     gene            complement(4521..>5686)
                     /locus_tag="PLY81_04820"
     CDS             complement(4521..>5686)
                     /locus_tag="PLY81_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF29580.1"
                     /translation="GLGVSYIKGISNHMDFASNLGGSFIDYPFLDAPKLGKEKFLLEL
                     DATLRLKLLTDKYIVVPYVSGGVGATALGGSHFGAYIPVSTGLQFNLGQSEAFLFTDV
                     SYRYPVTQKTGNYHLNYSIGLGAPLCDKPAAKPVEPPMPPKKVEPKDTDKDGIVDSLD
                     KCPTVYGIAKYQGCPIPDTDKDGINDEEDKCPTVYGVAKYQGCPIPDTDKDGINDEED
                     KCPTVAGLARYQGCPIPDTDKDGINDEEDKCPTEAGPAHNNGCPVLTEEKKQKVQLYA
                     KNIYFATGKSALLTKSYKSLDTIVALLKEKGNETLGLNIEGYTDNVGKPASNLKLSQS
                     RANAVMGYFKKKGIAENRLSAKGFGQESPVADNKTAQGRALNRRVELKLVEKQ"
BASE COUNT         1938 a          843 c          878 g         2027 t
ORIGIN      
        1 gcaaatgttt ttgcagcaaa tggttgcaaa gtatttttat ttgaagcatt aagaccaacg
       61 ccagaattaa gttttgccat aaggcattta ggctgtaaag caggtgtagt atgtactgca
      121 agccataacc caaaagaata taatggttat aaagcatatt ggaatgatgg aagtcaatta
      181 gttccaccac atgataaaaa tgttattaaa gaggtagagg caattgcaaa tgtagatgaa
      241 gttaaatgga caggtggcca agcaaatata acattattgg gtaaggaaat ggatgaagca
      301 tatattggta tggttaaagc tttaagcatt tatcctgatg ttattgctaa gcaacataat
      361 ttgaaaattg tttacacacc cattcacggt actggcatta caattgtacc agaaacttta
      421 aagcaatttg gttttagtaa tgtaacaatt gtggaagaac aagctacacc agatggcaat
      481 tttcctacag ttgtttatcc taatccagaa gaaactgaag caatgagtat tggattaaaa
      541 aaagctattg aaatagatgc agacattttg tgtggtacag atcctgatag tgatagagtt
      601 ggaataggtg taaaaaatca taaaggtgaa tgggttttaa tgaatggaaa tcaaacttta
      661 gttttagctt ttgcttattt aatagaagct agaaaattaa aaggtttggc aaaaccaaat
      721 gatatggtta tttctactat agtttctacc gatatggccc ataatgttgc acagcaaaat
      781 aatataagta gctatggtgt tttaacaggg tttaaatgga ttgctgaatt aattagagaa
      841 aaagaaaaag atgaaaatta tataattggt ggagaagaaa gttttggttt aatgattggt
      901 aatggaacta gagataaaga tgctgtaagt gctgttgcct tattgtgcga aatggcagct
      961 tatgaaaaag aacatggaag aagcttattt gataaactta ttgaactata tataaaatat
     1021 ggtttttatt acgaatcctt aattagtatt actaagaaag gtatgaacgg acaagcagaa
     1081 attgcagaaa tgatggaagg atatagaacc aacccaccta aagtaattaa tggtagcaaa
     1141 gtagtagaaa tattagacta tgaaaagcaa ataggaaaag attttgaaag tggcgatagc
     1201 tggaaaatag aattgcctaa gagtaatgta ttacaattca ttactgaaga tggaagtaaa
     1261 atatcggcaa ggccaagtgg aacagagcct aaaataaaat tttattttag tgtaaatact
     1321 aaactcaact ctaaaaatga gttcgacgaa aaatttgaat tacttcaaaa taaaattaaa
     1381 ggtataattg caagtttgaa cttgtagagt gaatacacta cacaataaaa aacctgaaac
     1441 ataatatttc aggtttttta ttgttgttca attataaact aagtaattat taaagatttg
     1501 gctttaatat ctaccaattc taaatcaaaa attaattctt tacctgctaa atgatggtta
     1561 gcatctaata ttacagattc ctctttaagt tcaataatgg taacattaaa ttgctgacct
     1621 gcaccattat gcattgttaa ttgcatgcca acttctggag ttaaatctgg aggaaattga
     1681 gctttaggaa aatccataat catatctggg ttactttcac cataggcttc tgctgcaggt
     1741 atgttaatag ttttcttttc tccaacactc atgccagtta caccattatc aaaaccagga
     1801 attaccattc cgctacctaa ttcaaattgt aatggttctc ttccttcact actgtcaaaa
     1861 gttgttccat ctgttaatct accatgataa tgaattttaa cagtatcgcc tttttttgct
     1921 tgttgcatta ttttattttt aaaatgaata atataccgtt ttctttcaaa taaatttatt
     1981 tgcacctatt cgggcagcca aagtacaccc taatttatta acctacaaga aattttaatt
     2041 tatgtgcata aaacagctta tagcaacata acaaacagaa tttctttacc agctattggt
     2101 atgtaaattg tattcccata acacttttat aaattatttt ttcctaaaat aaatatttca
     2161 caaaagcact taaaactctt atttggttat ttcatttata cggttacatt tgcaaaaaat
     2221 taaggactat gttttggaca ttagagttgg caagttatct tgaagaagcc ccttggcctg
     2281 ctacaaaaga tgaactaata gattatagca tacgtagtgg tgcccctatt gaagtagttg
     2341 aaaatttgca ggaattagat gacgaaggaa cagaagttta tgagagtatt gaagacattt
     2401 ggccagatta tccaagccaa gatgacttta tgtttaacga agatgaatat taaataatta
     2461 accacctttt gggtggtttt ttattgccat acttctaata taattttctt atctggttta
     2521 ccttcattag aaattacaat atcatacctc ttgggtatta ttttttctaa ctctttttcc
     2581 gttaatcctt tttttctaat aattagttta gatatattat tctcttgttc aatatctgtt
     2641 gctgttacag tataagagct atcatttaat gtattaaatg cataactaaa tggcattgaa
     2701 ctttcaggaa tctcaaaatc ttcctcttct aattctgtat cattaatttt tggcttactg
     2761 tttttaggag aatttttttt gctattaaat ggcttaccta cttcagaatt tgtagaaagt
     2821 tttctcttat tatcatttgt agaaatgctt tctatatctg ctggctcttt aaccaatttt
     2881 tcaaataata tatattgttc ttgttgatta ttgctacttt tattttgtgc taaaccagta
     2941 tgtgataagg ttttaacttc atttttaatt gtgttactta ataatctatt tgttgcttta
     3001 ttaattgatt cgttttgaat attaccagaa aagaaaaatg taaaagatag cagtgcaaat
     3061 aacataaaaa caaagccaaa tcgtaatgca gaataattat ttttagcact ttttgaattt
     3121 gtaattctat tgactctttt taataactca tttttatttg atatagcagc cataccaaat
     3181 tgatagggcc taaggttact cattgcaatt tgtagcaaag catttgcata taaattgggg
     3241 caatatttaa attgcgaaac ccagtcatca caactatttt ctctctcttg ttttatatgc
     3301 tcacttaaca atcttgaaaa aggattaaag aataaaacta tttcaactat tagaagtaaa
     3361 atattaacta aaaaatcaaa ccttttaata tgtgctaatt cgtgtaatat tactgcctct
     3421 atctgatagg tagataaatt attaatactt gctaaaggca ataatataat aggcttaaaa
     3481 tacccaattg ttagtggtgt gtttaccaat tcgcttaagt atattttaac ttctttattt
     3541 aatcctaatg caattgccat ttcattaaca aaaactctcc attctgcatt tactttatgt
     3601 agtttattaa ttcttaaatt gtttgtatga ttaaacccta taatgacttt tacaagtaga
     3661 attattgtta ctaataaata tacaacagat aagaaaggca atgctttttc tacttttaga
     3721 attaggggta taaaaaatga attaaaatta tttgattgaa ggaataggcc attctttaag
     3781 ttagatgaag tagaaaaaaa gttatagcag aaagtatata taaaactaca gaaaccaatt
     3841 atttgggcta caacagcaat tagatattta tgtttggaag gtaacttcaa gaaatttgtg
     3901 aaaagaatat aaagcaacca gattattgcc acttgccata aactatttat tagtgtatag
     3961 cctaaagatt gtaataaaaa ggatgtagca attggttgca taaataaagc tatttatttt
     4021 ttagcctatt caatattttt tctatttctt ccaattcatc tttacttggt ttgctgttac
     4081 ctaaagcttg cattgctaat tgtacagatg agcctccaaa aagcgtttca accattttat
     4141 gcataaattg tttctgtgta gattcttttg aaacgttggg tgtataaata tgtactttgc
     4201 ttgtatcatc tctaattacc aaactctttt caaacattat ttgtaataat tttagagttg
     4261 ttgtataacc actttctttg tgtaaactca aaatttcatg cacctctcta acactagcag
     4321 atttcttatc ccataatatt cttaaaattt ccaattcact ttcggtaggt ttaatttttt
     4381 gcttattaaa cataattacg attttattcg taagcaatat actttataaa ttttaattag
     4441 aattataaaa ttatataaat ggtagtaaaa acaaactgcc ccaatatttg gggcagtttg
     4501 aatataaatt aaagcgaata ttattgtttt tcaactaact taagttccac tcttctattt
     4561 agtgctctgc cttgtgctgt tttattatca gctacagggc tttcttgacc aaatcctttt
     4621 gcagataaac gattttctgc aatacctttt ttcttaaagt agcccattac agcatttgca
     4681 cgtgattgag ataattttaa attacttgca ggtttaccta cattatcagt gtagccttca
     4741 atatttaaac ctaatgtttc attgcctttt tcttttaata aagctacaat agtatctaat
     4801 gatttatagc tttttgttaa taaggcagat ttaccagtag caaaataaat atttttagcg
     4861 tataactgaa ccttttgttt tttctcttct gttaaaactg ggcagccatt attgtgtgca
     4921 gggccagctt ctgtagggca tttgtcttct tcatcattaa tgccatcttt atccgtatct
     4981 ggtataggac aaccttgata acgagctaaa ccagcaactg tagggcattt gtcttcttca
     5041 tcgttaatac catctttatc cgtatctggt attgggcaac cttgatattt tgcaacacca
     5101 tatactgtag ggcatttatc ttcttcatca ttaatgccat ctttatctgt atctggaatt
     5161 gggcaacctt gatattttgc aataccatac actgtagggc atttatctaa actatcaaca
     5221 atgccatctt tgtcggtatc ttttggttca acttttttag gaggcattgg aggttctact
     5281 ggttttgcag caggtttatc gcataaagga gcacctaagc caattgaata gtttaagtga
     5341 tagttaccag ttttttgagt tacagggtaa cgataagaaa catctgtaaa taaaaatgct
     5401 tcgctttggc ctaaattaaa ttgtaagcct gtactaactg gaatgtaagc accaaaatgt
     5461 gagccaccta atgctgttgc accaacacca ccagaaacat aaggaacaac tatatactta
     5521 tcagttaata attttagtct taaagtagca tctaactcta ataaaaactt ttctttaccc
     5581 aattttggag catctaaaaa aggataatca ataaaactac cgcctaaatt ggatgcaaaa
     5641 tccatgtgat tagaaatgcc tttaatatac gaaactccca atcctg
//
LOCUS       DAOQHP010000110         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7948_length_5651_cov_4.902252, whole genome shotgun sequence.
ACCESSION   DAOQHP010000110 DAOQHP010000000
VERSION     DAOQHP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7948_length_5651_cov_4.902252"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLY81_04825"
     CDS             <1..266
                     /locus_tag="PLY81_04825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF29581.1"
                     /translation="QGKKIDSTQLNQTYETRIKPNAPNFSYTKQQKEIYKRIGGTPFL
                     DQNYTVFGEMISGLNVLDKIAHVPTKPGDRPIKDVRMKIKMLN"
     gene            330..707
                     /gene="gcvH"
                     /locus_tag="PLY81_04830"
     CDS             330..707
                     /gene="gcvH"
                     /locus_tag="PLY81_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNF29582.1"
                     /translation="MNFPSNLRYTKEHEWVSIDGNTATIGITEFAQSELGDIVYVDIN
                     TVGNSLQANEVFGTIEAVKTVSDLFLPVAGTVSEVNKALEKNPELVNNDPYGEGWIIK
                     LTVNNISDIDTLLDSAGYAAVVG"
     gene            806..1297
                     /locus_tag="PLY81_04835"
     CDS             806..1297
                     /locus_tag="PLY81_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNF29583.1"
                     /translation="MPSFDIASKVDIQTLDNAINVVKKEITNRFDFRDSHVVIELNKK
                     DYTVLLEADSDMKLQQIIDVLISRAMKQGIDGNAFDFSKDAYPSGKVVKKNVSVKNGL
                     KQEDAKKIVKAIKDSGLKVQPAIMDNIIRVTAKKIDDLQAVIALCNNGNFGVPLQYEN
                     MKS"
     gene            complement(1311..1814)
                     /locus_tag="PLY81_04840"
     CDS             complement(1311..1814)
                     /locus_tag="PLY81_04840"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF29584.1"
                     /translation="MLAAKDYIEHYHLTPHPEGGFYKENYRSNLVLKQHSLPVTFTGN
                     RNVSTAIFFLLEQGNFSAFHKIKSDECWHFYAGQTLKVHIIYPNGVLETILLGNNTIL
                     GEQFQFVVPANCWFASEPAESTTFSFVGCTVAPGFDFDDFELAKAAHLMQLYPEHETI
                     IKRLCRC"
     gene            complement(1844..2500)
                     /locus_tag="PLY81_04845"
     CDS             complement(1844..2500)
                     /locus_tag="PLY81_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29585.1"
                     /translation="MSDKIKIIITDDHALFRQGVKAALSFRPDIEFLGEAENGMQLLN
                     MLKFLQPDVILLDIQMPIMDGIATLPEVKKVLPNVKVIMLTMHNEITLITKLMSIGAN
                     SYLTKDSESDVIYDAIKTCYENEFFFNEFTNRAMLSELRTQTAAPVKSDENAELSDKE
                     LQVLKLMCDEKSTKEIADIVEISPRTVEAIRDRLKIKTGAKSTAGLILYAVKHGLIEA
                     "
     gene            complement(2652..3143)
                     /locus_tag="PLY81_04850"
     CDS             complement(2652..3143)
                     /locus_tag="PLY81_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29586.1"
                     /translation="MRFIKLALISFLLLFIVVTAIGLLLPSTVIVSRAIDINAPKDSV
                     YNKVKNIYGWREWIAGMDKPEVNITSPTEANLFGTKVNITNINDTVITSTWLGRKGSL
                     QESVIRIIHYQNTPQIVVQWQFTESLKWYPWQRFGSMMNEAVTGKQLENNLAALKMLV
                     EKK"
     gene            complement(3166..4257)
                     /locus_tag="PLY81_04855"
     CDS             complement(3166..4257)
                     /locus_tag="PLY81_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF29587.1"
                     /translation="MQYKYSQLTALIAITKASLLAIIKNPGSIFFSLVFPLVFIWIFG
                     SFGNGGLNKINIAFSNQSDTNNAVFAILKNLPQIKIATYSNEALQKADLEKGNLAAII
                     KIASNDTANKHQYLINVTATNATSAEVAQLIPILENISIKINPDFKNYIQIEKNIYSI
                     RAYRQIDFILPGQIGFSILFSTLFGIAFTFYNLREQLILKRFYASPVNKLNILTGIGF
                     SRLFFQLINVVVLIIVGRFWLGFTLINGLVTFLEMILITFLMLIVLMGVGLIISSVVK
                     TDATISLLINLFALPQMLLSGTFFPITVFPDWMQNICSIFPLTHFNTAMRKISFEGLG
                     LVSIWQHIAVIGLWGLVVYILIYKIFKWE"
     gene            4420..4917
                     /gene="folK"
                     /locus_tag="PLY81_04860"
     CDS             4420..4917
                     /gene="folK"
                     /locus_tag="PLY81_04860"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNF29588.1"
                     /translation="MDKAKKEYIVYLLTGSNIGNKKENLYHAKHLVDKHCGNVSNHSP
                     IYQTAAWGLTNQPSFYNQALQLHTFLEPENLMQTLLNIEEMLGRVRKEKMGPRIIDID
                     ILLIENYTSNTNLLQVPHIALPFRKFALIPLADIAATVLHPILKITIAELLTKCKDDL
                     DVQKI"
     gene            4957..5589
                     /locus_tag="PLY81_04865"
     CDS             4957..5589
                     /locus_tag="PLY81_04865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF29589.1"
                     /translation="MKHHFITVEGNIGAGKTTLTHLLAKHFNARIILEEFADNPFLSK
                     FYDNPKQYAFPVELFFMAERYKQMKDMVHTKDLFQTVTVSDYLFTKCLLFAKVTLPEE
                     EFRLYQKLFDIIHQQLLFPDILIYLHAPVQKLQENIKKRNREYEQQIKDEYLYNLQET
                     YTSYIKQHNIKTIFIDASNADFLGNEKHFQVVLNALEKDIDSGQHYFSLP"
BASE COUNT         2090 a          858 c          803 g         1900 t
ORIGIN      
        1 ttcaaggaaa aaaaatagat tcaactcaac ttaatcaaac ttacgaaacc cgtataaaac
       61 caaatgctcc taattttagt tacaccaaac aacaaaaaga aatttataaa cgaattgggg
      121 gcacaccctt tttagatcag aattataccg tttttggcga aatgatttct ggcttaaatg
      181 tattggataa aattgcacat gttccaacaa aacctggcga caggcctata aaagatgtaa
      241 gaatgaaaat aaaaatgttg aattaattta atttcatttg aattaatagt aaaaaaacta
      301 atctttgcac aaataaaact ttcacaatta tgaactttcc ttcaaaccta cgttatacaa
      361 aagaacatga atgggttagt attgatggca atactgctac cattggcatt actgaatttg
      421 cacaaagtga attgggtgat attgtttatg tagatataaa tacagttggc aatagtttac
      481 aagccaacga agtgtttggt acaattgaag ccgtaaaaac tgttagcgat ttatttttac
      541 ccgttgcagg tactgtttct gaagtgaata aagctttaga aaaaaatccg gaattggtta
      601 ataacgaccc ttatggagaa ggctggataa taaaacttac tgtaaataat atttcagata
      661 tagatacttt attagatagt gcaggatatg ctgctgtggt aggataattt aataattact
      721 tttttatacc acataaatta caggcttata gtacttttaa acttgtagtt tatgtggttt
      781 attttttaat actaaaattt attgtatgcc atcttttgac attgccagca aagtagatat
      841 tcaaacctta gacaatgcta ttaatgtagt aaaaaaagaa ataactaata ggtttgattt
      901 tagagatagc catgtagtta tagaattaaa taaaaaagac tatacagttt tattagaggc
      961 tgacagcgat atgaaattac agcaaataat agatgtactt ataagcagag caatgaagca
     1021 aggtatagat ggcaatgctt ttgattttag taaagatgct tacccaagtg gtaaagtagt
     1081 aaagaaaaat gtaagtgtaa aaaatggttt aaaacaagag gatgcaaaaa aaatagttaa
     1141 agcaattaaa gacagtggct taaaagttca acctgctatt atggataata taataagagt
     1201 tactgctaaa aaaatagatg atttacaagc tgtaattgct ctatgtaata atggaaattt
     1261 tggtgtgcct ttacaatacg aaaatatgaa aagctaataa tccaaattca ttagcatctg
     1321 cataaccttt taattatagt ttcatgttca ggataaagct gcattaaatg tgctgcttta
     1381 gctaattcaa aatcatcaaa atcaaaacca ggagcaacgg tacagccaac aaaactaaat
     1441 gttgtacttt ctgcaggttc gctagcaaac caacaattgg caggcactac aaattgaaat
     1501 tgctctccca aaatagtgtt gttgcctaat aatattgttt ctaatactcc gttggggtaa
     1561 attatatgca cttttaaagt ttgccctgca taaaaatgcc aacactcatc gcttttaatt
     1621 ttatgaaatg ccgaaaaatt tccttgttct aataaaaaaa atattgctgt gcttacattt
     1681 ctgttacctg taaatgttac tggcaaactg tgctgcttta gcactaaatt agagcgatag
     1741 ttttctttat aaaacccacc ttctggatga ggtgttaaat ggtaatgctc tatataatct
     1801 tttgctgcta acaactataa agtttaagtt atgaaagaag agtttaggct tctattaagc
     1861 catgttttac agcatataaa attaagccag cagtactttt agctcctgtt ttaattttta
     1921 atctgtctct aatagcttca actgttcttg gactaatttc tacaatgtct gcaatttctt
     1981 tagtgctttt ttcatcacac attaacttta aaacctgcaa ttccttatcg ctcagttctg
     2041 cattctcatc actttttact ggtgctgctg tttgggttct caattcagat aacattgctc
     2101 ggttagtaaa ttcgttaaag aagaattcgt tttcgtaaca agtttttatg gcatcataaa
     2161 taacatcact ctctgaatct ttggttaaat agctattggc tccaatactc attagcttag
     2221 taattaatgt aatttcatta tgcatggtta gcataattac ttttacattg ggtaaaactt
     2281 tttttacttc aggtaatgta gcaattccat ccataattgg catctgaata tccaacaaaa
     2341 tcacatctgg ttgcaaaaat ttaagcatgt ttaaaagttg cattccattt tcagcttctc
     2401 ctaaaaattc tatatctggc ctaaaactta aagctgcttt tacaccttgt ctaaacaaag
     2461 catggtcatc tgttataatt atttttattt tatcgctcat agctataaca aatatataaa
     2521 cttttggaaa ggatagtaaa aatatcaaaa aaaaccttcc attcttacaa ggaaaatgta
     2581 ataaaaaata agaaactact aagtattaat actaatcatt aaagtaaaca ataaagtgtt
     2641 tactcatttt tctacttttt ttctacaagc atttttaaag cagctaaatt attttctaac
     2701 tgtttgcctg taactgcttc attcatcata ctaccaaaac gttgccaagg ataccatttt
     2761 aaagattctg taaactgcca ttgaacaact atttgaggtg tgttttggta atgaattatt
     2821 cgtataacac tttcttgtag gcttcctttt ctaccaagcc aggttgaggt aataacagta
     2881 tcgttaatgt ttgttatatt cactttggtg ccaaataaat tagcttctgt tggcgaagtg
     2941 atattcactt caggcttatc cattcctgca atccattctc tccaaccata tatgtttttt
     3001 actttattat aaacactgtc tttaggggca tttatatcaa tagccctaga aactataact
     3061 gtagagggta ataataaacc aattgcagta acaacaataa ataaaagcaa aaaacttatt
     3121 aaagccaatt ttataaaacg cataataaaa atttgaataa tgaatttact cccacttaaa
     3181 aattttataa attaaaatat aaactaccaa accccataaa ccaattactg caatatgctg
     3241 ccatatactt actaaaccta aaccttcaaa agatatttta cgcatagcag tattaaaatg
     3301 tgttaaagga aaaattgaac aaatattctg catccaatca ggaaaaacag taattggaaa
     3361 gaaagtgcca cttaacaaca tttgcggtaa agcaaataaa ttaataagca atgatatggt
     3421 tgcatctgtt ttaactacac tagatattat taagcctaca cccattaaaa caataagcat
     3481 taaaaaggta attaaaatca tttctaaaaa agtaaccaag ccatttatta atgtaaaacc
     3541 tagccaaaat cttcctacaa taattaatac aactacattt attaactgaa aaaacaaacg
     3601 actaaaacca ataccagtaa gaatattaag tttatttacc ggagatgcat aaaacctttt
     3661 taatattaat tgctctctta gattgtaaaa agtaaatgct atgccaaata aggtagaaaa
     3721 taatatggaa aaacctattt gacctggtaa aataaaatct atttgccgat aggctcttat
     3781 ggaataaata tttttttcta tctgaatata gtttttaaaa tccgggttaa tttttataga
     3841 aatattttct aaaatgggta taagttgtgc aacctctgca cttgtagcat tagtagctgt
     3901 tacattaatt aagtattggt gtttattggc agtgtcgtta ctagcaattt taataatagc
     3961 tgctaaattt cctttttcta aatctgcttt ttgtaaagcc tcattactat aagtggctat
     4021 tttaatttgt ggtaaatttt ttaaaattgc aaatacagca ttattggtat ctgactggtt
     4081 actgaaagca atatttattt tgttaagccc accattacca aacgatccaa aaatccaaat
     4141 aaaaaccaac gggaaaacaa gactaaaaaa aatggaacct ggatttttta tgatagccaa
     4201 taagcttgcc tttgtaatgg ctattaatgc tgttagctga ctgtatttgt attgcatatt
     4261 attattgaag ttgcaaactt atttatatta gttagattat ttttattttt tgcagataaa
     4321 aacaatctat tagcctaaaa tttatccctc aaatttttat ttaattaaat gtgttaaaac
     4381 aaaagataaa attatttttg tacattttat ataaacataa tggataaggc taaaaaagaa
     4441 tatatagttt atttgcttac aggtagcaat attggcaata aaaaagaaaa tttataccat
     4501 gctaaacact tagttgataa gcattgtggc aacgtatcta atcactcccc aatctaccaa
     4561 actgcagctt ggggcttaac caaccaacca agcttttata accaagcatt acaattgcat
     4621 acttttttag aacctgaaaa cttaatgcaa acattattaa atatagaaga aatgttaggc
     4681 agagttagga aagaaaaaat gggccccaga ataatagata ttgatatttt gctgattgaa
     4741 aactatacta gcaacactaa tttactgcaa gtaccacata ttgcattgcc ttttagaaaa
     4801 tttgcattaa taccactggc agatattgct gcaacagtat tgcaccctat attaaaaata
     4861 accatagctg aattacttac taagtgtaag gatgatttag atgtgcaaaa gatttaatta
     4921 ttatcatgct ttaggcttat aaatttgaag ccttgtatga agcatcattt tattactgtt
     4981 gaaggaaata ttggagctgg taaaactacc cttactcatt tacttgcaaa acattttaat
     5041 gcacgtatta tattagaaga atttgcagac aatcctttct tgtcaaaatt ttatgataac
     5101 ccaaaacaat acgcttttcc ggttgaatta ttttttatgg cagaaagata taagcaaatg
     5161 aaagatatgg tacatacgaa agatttattt caaacagtaa cagtatctga ttatttgttt
     5221 acaaaatgtt tgctttttgc caaagttacc ttacccgaag aggagtttag attatatcaa
     5281 aagttgtttg atatcattca tcagcaacta ctttttccag atatattaat atacttacat
     5341 gcacctgttc aaaaactaca agaaaatata aaaaaaagaa acagagagta tgagcaacaa
     5401 attaaagatg aatatttata taatttgcaa gaaacctata caagctatat aaagcagcat
     5461 aatataaaaa ctatttttat agatgcctct aatgcagatt ttttaggaaa tgaaaaacat
     5521 tttcaggttg ttttaaatgc attagaaaaa gatattgaca gtgggcaaca ttatttttct
     5581 ttaccttaaa tccaaggttt ataaattatt ctatacaact gaataatggc tgttataaaa
     5641 aacacaacac c
//
LOCUS       DAOQHP010000111         5632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_7984_length_5632_cov_3.596916, whole genome shotgun sequence.
ACCESSION   DAOQHP010000111 DAOQHP010000000
VERSION     DAOQHP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_5632_cov_3.596916"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..2109)
                     /gene="hscA"
                     /locus_tag="PLY81_04870"
     CDS             complement(253..2109)
                     /gene="hscA"
                     /locus_tag="PLY81_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNF29590.1"
                     /translation="MSKISINIATGSLQQAEIVVGIDLGTTNSLIAVIHPESKQAVAL
                     KEHDSSSLVPSVIYFDELNNAIVGDNAKQFLEKTPERTIFSSKRLMGKSYNDIKENEF
                     FFSYKVIDDDTDRLVKVQVGDKFYSPVELSSYILKELKARAEHILKTSVNKVVITVPA
                     YFNDAQRQATRDAGKLAGLDVLRIINEPTAASLAYGIGINQTEEKTIAVYDLGGGTFD
                     VSILTISNGIFEVLSTHGNTYLGGDDFDKVIVDYWSKEFNFSKDELQQNKQLMQGLRI
                     KAEEAKKHLSHNAEYRTNFNGDILTINKDKFEQLIQPFVNKTLDSCKQALKDSNITIN
                     EIDEVVMVGGSTRVPLVTNAVSNFFNKKVNNTLNPDEVVALGAAIQADILAGNNKEIL
                     LLDITPLSLGIETMGGLMDVLMPRNSKIPSKAARQYTTSKDGQINMRISVYQGERDLV
                     KDNRKLAEFNLTNIPAMPAGLPKVEVSFLIDADGILKVKAKELRSGVEQTIDVKPQYG
                     LTDDEVEKMLMDSITHAKDDIAIRALTEARTEGEQLLETTEKFIGKNAAQLSQDEMLA
                     TAQAMQTLQLALTMNDKNLIQTKIEELNEISRPYAERIMDNAVSSAMKGKVI"
     gene            complement(2311..3108)
                     /locus_tag="PLY81_04875"
     CDS             complement(2311..3108)
                     /locus_tag="PLY81_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNF29591.1"
                     /translation="MIQLIIKDKSTKLFIILSGFLIANALIAEVIGVKIFSLEETFGF
                     KPLAIKLLGEDLSLNLTAGVLLWPVVFIMTDIINEYYGVKGVRFLSYLSAFIIVYTFF
                     VFYASIHLSPANFFIISKQGSGVPNMQYAYEGVLGQGTKIIIGSITAFLLGQLIDAFT
                     FKKIKKITGEKKIWLRSTGSTLISQFIDSYVVLFIAFYIGSRIGAKNGDFVWSFQLFL
                     AVGTLNYIYKGLVAIILTPVIYLLHYFIEKYLGHEKATAMKEAAMNS"
     gene            3443..>5632
                     /locus_tag="PLY81_04880"
     CDS             3443..>5632
                     /locus_tag="PLY81_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29592.1"
                     /translation="MKNINWKKILPHVYAIAIFLIVAIIYCKPALEDKVLHQADVTQW
                     KSMAQNQQAVLDATDKVPLWSNGMFGGMPGYMIKGWSNNMVGYYFMDLISLNIPKPFL
                     FFFLASICFYFLTQVLRANTWVGTIISLCYAYATYNVVIVAEGHDTKMLSLAVLPGLI
                     GAIQLIFNKKYLWGITLTALFSSVLVAQKHYQIAYYGILIVAFMSIAFVVQCILKKEL
                     KHLLFAGLFAIAGVALGALQNAVILLPDNEYTKESIRGGSQLANDHTKTTKEGLTKEY
                     AFSYSMYKTEPFVMLIPRMFGGSNKMELEPEKSKAVANLQAMNPQIAQQLQSALSFYW
                     GGIGGTSGPPYVGAIICFLALIGFVILNNKHKWWILALTVLTIMMSWGSFFEGFNNFL
                     FNHLPLYNKFRAPSMILVVPTILLNIMAVLTLQKIIEAKDKAALFIQYKKGLMITGGV
                     FIILLLMYFGFDYKGESDTNLINNINNIPDAETKAVYLDAGKKMLNGLVEDRKSLFMS
                     DILRALFYVAVAALAIWLLIKNKAKEWMLLASIGLFSFIDIMTVDVKYLNSDNYQYKE
                     DYETSFNPTPADKFILQDKSDYRVFDVSNGAQAAINYGARASYYHQSVGGYHPAKLSI
                     FQDLAENQLYNYPNCKPVLNMLNTKYIIHGTSKADQVEINRDACGPVWFIKALKIVGT
                     PNEEMNALTNLSVKDTAVIGKGFEQIANTKFSYDTTANITVEMKQNDV"
BASE COUNT         1930 a          931 c          807 g         1964 t
ORIGIN      
        1 attctgagca cccattattt atgtcattct gagcgaagcg aagaatctac tgagcaccca
       61 ttatttatgt cattctgagc gaagcgaaga atctcctgag ctcccattat ttatgtcatt
      121 ctgagcgaag cgaagaatct actgaaaaaa gtactaatgc acaagtagat gtttcgcttc
      181 gctcaacatg acaatacttt ttgtttcact ttgctcaaca tgtcaatagg aaaaatacaa
      241 agcaaaaacc aactaaatta cctttccctt catagcacta cttacagcat tatccattat
      301 acgttcggca taagggcggc ttatttcatt cagttcttct attttggttt gaattaaatt
      361 tttatcattc attgttaaag ccaattgtaa tgtttgcatt gcttgtgcag tagccaacat
      421 ttcatcttgc gaaagttgtg ctgcattttt accaataaat ttttctgttg tttctaacaa
      481 ttgttctcct tcagttctag cttctgttaa agctctaatt gcaatatcat cttttgcatg
      541 tgtaatgcta tccatcagca ttttttcaac ttcatcatcg gttaagccat actgtggttt
      601 tacatcaatt gtttgttcta caccactcct taactctttt gctttcactt ttaaaatacc
      661 atctgcatca atcaaaaaac ttacttctac ttttggtaag cctgcaggca tagctggtat
      721 attggttaaa ttaaattcag ctaactttct attgtctttt accaaatctc tttccccttg
      781 atacacagaa attctcatat ttatttgtcc atctttagag gttgtatatt gccttgcagc
      841 tttagaagga attttagaat tacgtggcat taaaacatcc attaagcccc ccatagtttc
      901 tatacctaag ctcaagggtg taatatctaa caataatatt tctttattat ttcctgctaa
      961 aatatctgct tgaatagctg cacctaaagc aactacttca tctgggttta gtgtattgtt
     1021 tactttttta ttaaaaaaat tactaaccgc atttgtaact aatggcactc ttgtgctacc
     1081 gccaaccata accacttcgt caatttcatt tatggtaatg tttgaatctt ttaaagcttg
     1141 tttacaacta tctaatgttt tatttacaaa tggttgaata agttgttcaa atttatcttt
     1201 attaattgta agtatgtctc cattaaaatt tgtacggtat tcagcattat ggcttaaatg
     1261 tttttttgct tcttctgcct ttatacgcaa gccctgcatt aattgcttat tttgttgtaa
     1321 ttcatcttta gaaaaattaa attctttgct ccaataatca acaataactt tatcaaaatc
     1381 atccccacct aaataagtat ttccatgggt gcttaatact tcaaaaatgc cattacttat
     1441 ggtaagaata gacacatcaa atgtgccacc acccaaatca taaactgcaa ttgttttttc
     1501 ttctgtttgg ttaattccaa ttccatatgc aagacttgct gctgttggct cattaattat
     1561 tcttaaaaca tctaaaccag ctagtttacc agcatctctt gttgcctgtc gttgtgcatc
     1621 gttaaaataa gcaggcaccg ttattaccac tttgtttaca gaagttttta aaatatgctc
     1681 tgctcttgct tttaattctt ttaaaatata agaggaaagc tcaactggcg aataaaactt
     1741 atcccctacc tgcactttca ctaatctatc agtatcatca tcaattactt tataggagaa
     1801 aaaaaactca ttttctttaa tatcattata gcttttcccc attaatcttt tcgaagaaaa
     1861 aatggttcgt tcaggagttt tttctaaaaa ttgctttgca ttatctccaa caattgcatt
     1921 atttaattca tcaaaataaa taacacttgg tacaaggcta ctgctatcat gctcttttaa
     1981 ggcaactgcc tgcttgctct ctggatggat aacagctatt aaactatttg tagtacctaa
     2041 atctatgcct acaacaattt ctgcctgctg taagctacca gttgcaatat tgatagaaat
     2101 ttttgacata gctaatctga aactgaagga ttattaattt cagcaaaaat aatgattgca
     2161 aatgtagagt aaccgaaatg gtttatgaga taagaaagaa aaaacaatac tactataatt
     2221 gtagtttaat tctataaact aattagtatc aagaatagta actgaaaaaa tcaaactcta
     2281 aaaagcatga taatgaatat ttagaaattg ttaactattc attgcagctt ctttcatggc
     2341 tgttgctttt tcatgcccta aatatttttc aataaaataa tgtagtaaat aaattacagg
     2401 tgtaagtata attgctacca accctttgta aatataattt aatgtaccaa cagctaaaaa
     2461 aagttgaaaa ctccaaacaa aatcgccgtt tttagcccct attctagagc caatataaaa
     2521 agcaataaac aataccacat aactatctat aaattgagat attaaagtag agcctgttga
     2581 tcttaaccaa attttctttt ctcctgtaat tttttttatt tttttaaatg taaacgcatc
     2641 aattaattgt cctaataaaa atgcagttat gctaccaatt ataatttttg ttccttgccc
     2701 taaaacacct tcataagcat attgcatatt aggcacgcca ctaccttgct tacttataat
     2761 aaaaaaattg gctggagata aatgaataga tgcataaaaa acaaaaaaag tatatacaat
     2821 aataaatgca cttaggtaag ataaaaaccg cacacctttt actccatagt attcattaat
     2881 aatatcagtc attataaaaa ctactggcca aagtaaaaca cctgcagtta aattaaggct
     2941 taaatcctct ccaagcaatt taatagctaa tggtttaaac ccaaaagttt cttctaacga
     3001 aaaaatttta accccaatta cttctgcaat taaggcattg gcaattaaaa atccacttag
     3061 tataataaac aacttagttg atttatcttt aataattaac tgaatcataa ttgaagataa
     3121 aatgaattaa aaatttgggc tattaaaaaa attaaattaa caacaccaag gcttactgga
     3181 attatttttt ttcttacaac cacacttacc aaccataaaa aactagccat aatatttaaa
     3241 aaaatagcaa aaaaaccaag aacaaccaag gtacttacta aaaatgcagg tagttgttct
     3301 acactaaaat atctagctat tacagagaat acataacaca cattacatat taaagcaatt
     3361 ctactaaaaa ataaaagcac cctcatattt ttattttgaa ctgaaaaata acattaattt
     3421 gcttgttata aaaagaaaat aaatgaagaa tataaattgg aagaaaattt taccccatgt
     3481 ttatgccatt gcaatttttt taatagttgc aattatttat tgtaaacctg ccttagaaga
     3541 taaggtttta catcaggcag atgttacaca atggaaatct atggcacaaa atcaacaagc
     3601 agttttagat gccactgata aggttccact ttggtcaaat ggtatgtttg gtggtatgcc
     3661 aggttatatg attaaaggct ggagtaataa tatggttggt tactatttta tggatcttat
     3721 ttcgctaaat attccaaagc cttttctatt cttcttttta gcaagtattt gcttttactt
     3781 tttaacccaa gtattaagag ctaatacttg ggtaggaact ataatttcat tgtgttacgc
     3841 ttatgcaact tataatgtag ttattgttgc agaagggcat gatacaaaaa tgctatcgct
     3901 tgcagtacta cctggattaa taggtgccat tcaacttata tttaataaaa aatatttatg
     3961 gggcataaca ttaactgcac tattctctag tgtactagtg gcacaaaaac actatcaaat
     4021 tgcatattat ggtattttga ttgttgcttt tatgagtatt gcttttgtgg ttcaatgtat
     4081 tcttaaaaaa gaattgaaac acttattatt tgcaggttta tttgctatag ctggtgttgc
     4141 attaggggca ttacaaaatg ctgttatatt attgccagat aatgaatata ccaaagaatc
     4201 tattagaggt ggttcgcaat tagccaatga ccataccaaa actactaaag aaggtttaac
     4261 taaagaatat gcatttagtt acagcatgta caaaacagag ccttttgtaa tgcttattcc
     4321 aagaatgttt ggaggaagta ataaaatgga attggaacca gaaaaatcta aagcagtagc
     4381 taatttacag gcaatgaacc cgcaaattgc tcaacaatta caaagtgctt taagttttta
     4441 ttggggagga attggtggca cttctggccc accttatgtt ggtgcaatta tttgttttct
     4501 tgcattaatt ggttttgtta ttttaaacaa caaacataaa tggtggatat tagcccttac
     4561 agttttaact attatgatga gttggggaag cttttttgaa gggttcaaca actttttatt
     4621 taatcatttg cctttgtata acaagtttag agcacctagt atgattttag tagtgcctac
     4681 cattttatta aatataatgg ctgtacttac ccttcaaaaa attattgaag ccaaagataa
     4741 agctgcactt tttatacaat acaaaaaagg cttaatgatt acaggcggtg tttttattat
     4801 tttattactg atgtattttg gctttgatta taaaggagaa tctgacacca acttaataaa
     4861 caacatcaac aatattcctg atgcagaaac caaagctgtt tatttagatg ctggaaaaaa
     4921 aatgttgaat ggtttagtgg aagacagaaa aagtttattt atgagcgaca ttcttagagc
     4981 attattttat gttgctgtag ctgcacttgc tatttggcta ttaataaaaa ataaagctaa
     5041 agagtggatg cttcttgcat ctattggatt attttctttt attgatatta tgacagttga
     5101 tgttaaatat ttaaatagcg ataactacca atataaagaa gattatgaaa cttcttttaa
     5161 ccctacacct gcagataaat ttattttaca agacaaatca gattatagag tttttgatgt
     5221 aagcaatggt gcacaggctg caataaatta tggtgcaaga gcatcttatt atcatcaatc
     5281 tgttggaggc tatcatcctg ccaaattgag catttttcaa gatttggccg aaaatcaatt
     5341 atacaattat cctaactgta aacctgtttt aaacatgcta aataccaagt atattataca
     5401 tggtacaagc aaagctgatc aggtagaaat taatagagat gcttgtggac ctgtttggtt
     5461 tattaaggca ttaaaaattg ttggtactcc taatgaagaa atgaatgctt taaccaactt
     5521 atctgtaaaa gatactgcag ttataggaaa aggatttgag caaatagcca atacaaaatt
     5581 tagttacgat actacagcaa atattactgt agaaatgaaa caaaatgatg ta
//
LOCUS       DAOQHP010000112         5566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8129_length_5566_cov_3.501179, whole genome shotgun sequence.
ACCESSION   DAOQHP010000112 DAOQHP010000000
VERSION     DAOQHP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_5566_cov_3.501179"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLY81_04885"
     CDS             complement(<1..430)
                     /locus_tag="PLY81_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNF29593.1"
                     /translation="MTYLIILSIAFGISFLGQIVLSNMNMAATQLSVQEGFNKAWKFG
                     IGVAIVEIIYLRLALTGMSLVTQYKYIFKLLGWITVVVFLILGVLAFISAYKQVVEKK
                     GLLINNKINRFLLGVTISALNPVQIPFWFLWSTQLIQNNVL"
     gene            complement(472..1518)
                     /locus_tag="PLY81_04890"
     CDS             complement(472..1518)
                     /locus_tag="PLY81_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29594.1"
                     /translation="MKKILQKIADWEQWNFYVIYTPLVFVWLYYFIRTGKFWYFSNVN
                     PSLQFSGLEGETKKEMFEQLPLHTFPTTIYIKPACNFMEVVQQVNKAALHYPVAVKPD
                     IGTKGLCFRKIENEEQLKQYHATVPFEYLVQSFIEYPLELSVFYIRYPNSTKGKITGL
                     IAKEYLQVKGNGKQTLLELIQQHPKAKFRLNELTVKHAEHLQDIIENNEPYFLSITGN
                     HNRGATFVNLYQEIDQQLTNVFDEISNYTKHFYYGRFDIKTTNLKDLKAGKNISILEF
                     NGTGAEPNHIYDCNMSYFKALKTIAAHWADMYNIGRINYHKGIPYISFKQGRKFLADA
                     FTAYAVLEEYEKIC"
     gene            complement(1605..2660)
                     /gene="hisC"
                     /locus_tag="PLY81_04895"
     CDS             complement(1605..2660)
                     /gene="hisC"
                     /locus_tag="PLY81_04895"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNF29595.1"
                     /translation="MAFNINHLLRPNIEKIVNQKNNISKNEIKISLHKNENSLGSPLK
                     KWYNRYPDDEQNNLKQAIAALKNVTTENVFLANGTNESIEILYKCFCAPEKDNVIICP
                     PTYSQFENFADIYNIEVRKAVLLENFQLDLITLENLVDTHTKIIWLCSPNNPTGNSLN
                     LNDIEFVLNNFNGLVVVDEAYINFSAQKSLTALLKEYDNLIILQTFSIAWGLAGLRLA
                     IALSNPNTITILNKVKNTNTISTVSKETALKAFEEIGQVNDMIKLLVEMRIALADVFK
                     QIPFIIKVHDSDANFILIEVDNASKLTDYLLSVQIAVKNVSHLPNCHNCIRITVGNEK
                     ENTDLVDALSNYLEMHY"
     gene            2750..3982
                     /locus_tag="PLY81_04900"
     CDS             2750..3982
                     /locus_tag="PLY81_04900"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNF29596.1"
                     /translation="MKQFQVPTNYRSSLISAVKNKRKQEDKLKKDFTPTLLDFGNLQI
                     YLARHFGFCYGVENAIEIAFRTIDENPGKKIYLLSEMIHNPQVNKDLKERGVQFLQDT
                     YGKQLIPFEDINKEDIVLIPAFGTTLKIEEMLHKKGAGTDKFNTTCPFVEKVWNRGEQ
                     IARKGYSIIVHGKPKHEETRATFSHAASMAPTVVVNDMDETIELAKYITGEKNEEQFY
                     TEFEGKYSQNFNVKKDLLKIGVINQTTQLATDTQAIADYLKMVMIKKFNLDAKSVSNH
                     FADTRDTLCYATNDNQSAVSGMLNTPADLALVIGGYNSSNTSHLVELCEQKLPTYFIE
                     SAAKIISKSELIDCNWKTKEIKTVQHYLPQTTPVKILITSGASCPDALVEEVIRKIAS
                     FYNAEEKIEEMEKIFSVN"
     gene            4129..>5566
                     /locus_tag="PLY81_04905"
     CDS             4129..>5566
                     /locus_tag="PLY81_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNF29597.1"
                     /translation="MANRLKKKNIETPNPEELTTDKVADVTVAEVVKDERTTKIMGAV
                     SLLFTIFLFVAFTSYLFTWQEDQDKVQKFGVKIFSISDVQVSNLLGLLGAYIAHVFIY
                     KGFGIASFLFCTFFFVLGVNLLFGKKIFSIKRNIKYLITGLIVLSVAFTFMAAKAEFS
                     WGGAVGELLNSWLIKWIGKIGTGTILLLSVFAYFIWRFNPTFTYPEQFFKNIFAPKPK
                     KEIPTEDVAETQDNETINKLTVDSNDEEELQNFELKENINKEPTSNNRLKTEGNAVTV
                     IMPDVEENNLPIFDIVDKPEIELNEVLVAEAPLSVNDKFANAENSNHDLSLEIKPITD
                     ENNSVEEQEIETTNKIEEELDEVDIDEQEENSALEVRSDELATFNYDPTLDLRQYQYP
                     SLDLLETHGSEKIIHDPTELETNKNQIIATLKNYDISIQRISATVGPTVTLYEIVPAP
                     GVRISRIKNLEDDIALSLAALGIRIIAPI"
BASE COUNT         2020 a          841 c          824 g         1881 t
ORIGIN      
        1 gtaatacatt attttgaata agttgagtgc tccataaaaa ccaaaatggt atttgaactg
       61 ggtttaaagc actaatagtt acacccagca aaaatctatt aattttatta tttattaaaa
      121 gccctttttt ttctaccact tgtttatatg cacttataaa agcaagtaca cctaatatta
      181 aaaaaaccac aactgtaatc caacccaata atttaaaaat atatttatac tgagttacca
      241 aactcatgcc tgttaaagcc aatcttaaat aaataatttc aactattgca actccaatac
      301 caaatttcca agccttatta aagccttcct gcacacttaa ttgtgtagct gccatgttca
      361 tattacttaa aacaatttgc cccaaaaaac taattccaaa tgctattgat aaaataatca
      421 aataagtcat aatagtttaa aattagttat gaaattttta agataaatga attagcaaat
      481 tttttcgtat tcttctaaaa cagcataagc tgtaaaggcg tcagctaaaa attttcttcc
      541 ttgtttaaaa gaaatatagg gtatgccttt atggtaattt attctaccaa tattatacat
      601 atctgcccaa tgtgcagcaa ttgtttttaa agctttaaag taactcatgt tgcaatcata
      661 aatatgattt ggctctgcac cggtgccatt aaactctaat atgctaatat ttttacctgc
      721 ttttaaatct tttagattag tagttttaat atcaaatcta ccgtagtaaa aatgtttagt
      781 ataattactt atttcatcaa aaacattggt aagttgctga tctatttctt ggtataaatt
      841 tacaaaagtg gcacccctgt tatgattgcc tgtaatactt aaaaaataag gttcattatt
      901 ttcaataata tcttgtaaat gttctgcatg tttaacagtt aattcattta acctaaattt
      961 tgctttaggg tgttgttgaa ttaactctaa taaagtttgt ttaccattac cttttacttg
     1021 taaatattct tttgcaatta agccagtaat cttgcctttt gtgctgttag ggtaacgaat
     1081 ataaaacaca cttaattcta aagggtattc tataaaagat tgtacaaggt actcaaaagg
     1141 cactgttgca tgatattgtt ttagctgctc ttcattttct atttttctaa aacataaacc
     1201 ctttgtgcca atatctggtt ttactgcaac aggataatgt aaagctgctt tatttacttg
     1261 ttgaaccacc tccataaaat tacaagctgg cttaatgtaa atggttgttg gaaaagtgtg
     1321 taacggcaac tgctcaaaca tttctttttt agtttcgcct tctaaacccg aaaattgtaa
     1381 acttggattt acattagaaa aataccaaaa cttaccagtt cttataaaat aatacaacca
     1441 aacaaaaacc aatggagtat aaattacata aaaattccac tgctcccaat ctgcaatttt
     1501 ttgcaaaatt tttttcaaat tctaattttt attggcggca aaaatagtta gttagcatgt
     1561 attaaaatac tgttttttag taattaacta catagtttcc tatattaata atgcatttct
     1621 aagtaattac tcagggcatc tactaaatct gtattctctt tttcatttcc aacagttatt
     1681 ctaatacagt tatggcaatt gggtaaatga ctcacatttt taacagcaat ctgtacactt
     1741 aacaaataat cagttagctt actggcatta tctacttcta ttaaaataaa attggcatcg
     1801 ctatcatgta ctttaataat aaaaggaatt tgcttaaaaa catctgccaa agcaattctc
     1861 atttccacca aaagttttat catatcgttc acctgcccaa tttcttcaaa agcttttaag
     1921 gctgtttctt tacttactgt gctaatagta ttggtgtttt taactttgtt taaaatagta
     1981 atagtattgg ggttagatag agcgatggct aaccttaaac cagccaaacc ccacgcaata
     2041 ctaaaggttt gaagtataat taagttgtca tattctttta gtaaagcagt taaagacttt
     2101 tgtgcagaaa aattaatata ggcttcatct actaccacca aaccattaaa gttatttaaa
     2161 acaaattcaa tatcgtttaa gtttaaactg ttgcctgttg ggttattggg cgaacagagc
     2221 caaataattt tggtatgtgt atctactaaa ttttctaatg taatcaaatc caactgaaaa
     2281 ttttctagta gtactgcttt tcgtacttca atattataaa tgtctgcaaa attttcgaat
     2341 tgactataag ttggtggaca aataataaca ttatcctttt caggggcaca aaagcatttg
     2401 tataaaattt ctatactttc attagtacca ttggctaaaa aaacattttc ggttgttaca
     2461 ttttttaaag cagcaatagc ttgttttaaa ttattttgtt catcatcagg atatctattg
     2521 taccattttt ttaaagggct acctaagctg ttttcatttt tatgtaaact aatttttatt
     2581 tcgtttttag aaatattatt tttttggttt acaatttttt caatgtttgg tcttaaaaga
     2641 tggttgatat taaaagccat aactgctttg tatatgataa ttttaaaata aaaggctaaa
     2701 ttaaatgctt taagcatact tcgtacattt gcacaagttc aaataaatta tgaaacaatt
     2761 tcaagtacct actaattata gaagcagcct tataagtgct gtaaaaaata aacgaaaaca
     2821 agaagataaa ttaaaaaaag actttacccc aaccttatta gattttggaa acttacaaat
     2881 ttatcttgct cgtcattttg gtttttgtta tggtgtagaa aatgcaattg aaattgcttt
     2941 tagaacaata gatgaaaacc caggcaaaaa aatttattta cttagtgaaa tgatacataa
     3001 cccacaagta aataaagatt taaaagaacg tggtgtacaa tttttacaag atacctatgg
     3061 caaacagctt attccttttg aagatataaa taaagaagat attgttttaa ttcctgcatt
     3121 tggtactact ttaaaaatag aagaaatgct acacaaaaaa ggtgcaggta ccgataagtt
     3181 taataccact tgcccatttg tagaaaaagt gtggaaccgt ggtgagcaga ttgccagaaa
     3241 aggctatagc attattgtgc atggcaaacc caagcatgaa gaaacaagag ccactttttc
     3301 tcatgcagca tcaatggcac caacagttgt ggtaaacgat atggatgaaa caatagaatt
     3361 agcaaaatat attacaggag aaaaaaatga ggaacaattt tatactgagt ttgaaggcaa
     3421 atacagccaa aactttaatg ttaaaaaaga tttattgaaa attggggtaa tcaatcaaac
     3481 aacacaatta gctacagata cacaagccat tgcagattat ttaaaaatgg taatgataaa
     3541 aaagtttaac ctcgatgcta aatcggtgtc taatcatttt gcagatacaa gagatacgct
     3601 ttgttatgcc acaaacgata atcaatctgc tgtatctggt atgttaaata cacctgcaga
     3661 tttggcattg gtaattggtg gctacaattc atcaaacact tcccatttag tggaactttg
     3721 tgaacaaaaa ttaccaactt attttattga atctgctgca aaaattatca gcaaatcaga
     3781 actgattgat tgcaactgga aaaccaaaga aattaaaact gtgcagcatt atttaccaca
     3841 aactacacca gtaaaaattt taataacaag tggtgcaagc tgccccgatg cattggttga
     3901 agaagtaatc agaaaaattg ccagctttta taatgccgaa gaaaaaatag aggaaatgga
     3961 aaaaatattt tcagttaact agtattcctt cttccttttt gcaataaaaa aattccataa
     4021 gttatccact ttgggcattt aatgtagttt ttaaccaaaa gtaatcccca tggttgttat
     4081 aactgtagat gcatttgtag cttaggcaat atttaatttg cctacattat ggccaatcgt
     4141 ttaaaaaaga aaaatattga aactcccaac cccgaagagt taacaacaga taaggttgca
     4201 gatgttacag tagccgaagt tgtaaaagat gaacgtacta ctaaaataat gggtgctgtt
     4261 agtttattgt ttactatttt tttatttgtt gcttttacat cttatttatt tacctggcaa
     4321 gaagatcaag ataaagtaca aaaatttggc gtaaaaattt tctcaatatc tgatgttcag
     4381 gttagtaatt tattagggtt attgggtgct tatattgctc atgtttttat ttacaaaggc
     4441 tttggcattg catcgttttt attttgcact ttcttttttg tattaggagt aaatctactt
     4501 ttcggaaaaa aaatattttc aattaaaaga aatattaaat acttaattac aggtttaata
     4561 gttttaagtg ttgcctttac ttttatggca gctaaagctg agtttagctg gggtggtgct
     4621 gttggagagt tattaaatag ttggttaatt aaatggattg gaaaaattgg aacaggcaca
     4681 atattgctac taagtgtttt tgcatatttt atttggcgtt tcaatccaac atttacatac
     4741 cccgagcaat tctttaaaaa tatttttgct ccaaagccta aaaaagaaat tcctacagaa
     4801 gatgttgctg aaacacaaga taatgaaaca ataaacaaac ttactgttga tagtaatgat
     4861 gaagaagaat tgcaaaattt tgaactaaaa gaaaatataa ataaagagcc aaccagcaat
     4921 aacagattaa aaacagaagg taatgcagta actgtaatta tgccagatgt ggaagaaaat
     4981 aatttgccaa tttttgatat tgtagataaa ccagaaattg aattgaacga agttttagtg
     5041 gcagaagctc cgctatcagt aaatgataag tttgcaaatg cagaaaacag taaccatgat
     5101 ttaagtttag aaataaaacc aataactgat gaaaataata gtgttgaaga acaagaaatt
     5161 gaaacaacaa ataaaataga ggaagaattg gatgaagtgg atattgatga acaagaagaa
     5221 aactctgcat tagaagtaag aagcgatgaa ttggcaactt ttaattatga ccccacttta
     5281 gatttaagac agtatcaata ccctagttta gatttattag aaacacatgg tagcgaaaaa
     5341 attattcatg acccaaccga actggaaaca aacaaaaatc aaattattgc tactcttaaa
     5401 aattacgata tttccattca gcgtatttct gccactgttg gcccaacagt tactttatac
     5461 gaaatagtac cagccccagg tgtaagaatt agcagaataa aaaatttaga agacgatata
     5521 gctttaagtc ttgcagcact tggcattcgt attattgcac caatac
//
LOCUS       DAOQHP010000113         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8160_length_5555_cov_3.948727, whole genome shotgun sequence.
ACCESSION   DAOQHP010000113 DAOQHP010000000
VERSION     DAOQHP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8160_length_5555_cov_3.948727"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLY81_04910"
     CDS             complement(<1..795)
                     /locus_tag="PLY81_04910"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNF29598.1"
                     /translation="MSKKMRTLTEPFKIKMVEPLRITTEEYRQNALEKAGYNPFLLRS
                     DDVFIDLLTDSGTGAMSDKQWGGIMMGDESYAGARSWEHLQTVVQNLTGMQYVLPTHQ
                     GRAAERILYSILGGKGKVFISNTHFDTTRANIEYVGSEAYDIVCKEAEDVESYHAFKG
                     NIDLEKLETLIQQHGKNIAAIIMTVTNNSSGGQPVSMKNMKDVRAICTKYGLMMVIDG
                     CRIAENSYYVKHREEGFENKTYKEIAQEMLNLGDAFIMSAKKDGMVN"
     gene            complement(946..1815)
                     /locus_tag="PLY81_04915"
     CDS             complement(946..1815)
                     /locus_tag="PLY81_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNF29599.1"
                     /translation="MFITLRTTVRSYFVGIGQIMLQENAITGLLFLIGIFYNSVIMGI
                     TIFFATIIGTSTAKLLKYDVNNINKGLYGFNAALVGIAGIVFLKIKILSISILIIACV
                     LSTILHNFFIKKKLPVYTLSFVTVTYSIIFFIKTFTPNLLILNEGCSPAYNLNFDYIF
                     KGFGQVIFQENAVSGIVFFIAIFISSPIAAIYAITFSELSGLIAISLSIPLNLVNAGL
                     FSYNALLCAIAFANRKLFTIFWVLLASSLSLIFSLLMNQIQIIQLTLPFVLACFIVST
                     LKNRNHINKQLLN"
     gene            complement(2001..2429)
                     /locus_tag="PLY81_04920"
     CDS             complement(2001..2429)
                     /locus_tag="PLY81_04920"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF29600.1"
                     /translation="MIVAKKFKWEAAHRLPWHIGKCKQLHGHSYKMIVEFEGEPNENG
                     MIIDFNDIKNMLDPLVKQIDHATIISKNDIELKEIFEAKQWKYFLLPYDSTAENLCAF
                     FLETIINENKQVLKENNISKVGIKIFETETAYAYSTKLVD"
     gene            2707..3543
                     /locus_tag="PLY81_04925"
     CDS             2707..3543
                     /locus_tag="PLY81_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF29601.1"
                     /translation="MKIVPHLIFVTITLFVISAKKLHAQKAGNEIFKETCSACHTVGK
                     GKLIGPDLANVHNHLTLDYFTKFVKSSQSVIKSGDKYADSIFNAYGKMAMPDHPQLSE
                     EQIKSIYDYLKEASGGGAEEAIPEEELLPGDIERGRALFVGNLRFTNSGASCNSCHNV
                     NIAGLITGGALAKDLTQSVTRLTETGVAGVIKGLPYPQMKETYAMHPITPQEVADLTT
                     FLVYVDEKSAIKTGNYTGNYMLLGGGGGIVFLLSLYSFFWFKRKRQAVNKSVFSRQIK
                     SA"
     gene            3691..>5555
                     /locus_tag="PLY81_04930"
     CDS             3691..>5555
                     /locus_tag="PLY81_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNF29602.1"
                     /translation="MSWIKDIISPQTRKWEEFYRNRFQHDRIVRSTHGVNCTGGCSWA
                     IHVKEGIVVFETQQLDYPLIDDKLPPYEPRGCQRGISYSWYLYSPLRIKYPLIRGTLL
                     DAFREEKQKVNGDPMKAWENLQNNKEQRKKYQNSRGKGGFRRTTWDEVLEIMAAANIH
                     TAKKYGPDRVIGFSPIPAMSMLSYASGARFLQLFGGVNLSFYDWYCDLPPAFPEIWGE
                     QTDVCESADWYNAKMIADMGTNLNMTRTPDCHFFAESRHNGTKTVVFSPDFSQLCKYA
                     DQWVPLHAGSDGAFWMSVTHVILKEYHFEKQTPYFIDYVKRYTDSPMLVHLTKTENGF
                     TAGRMVRANELSKWKNIENGDWKFLSIDEGTGEFVVPKGTMGYRWDKAGGKWNMKYEC
                     GETDKSYNPILSFINNNDDVLEVTFTEFGLSKTAERGVPVKWLDTENGRVPVTTVYDL
                     TMAQYGVDRGLKGDYPTDYNNPDAAYTPAWQEIFTGIDSKTLLQFAREWAETANTTEG
                     KCMILVGAGVNHWYHQNLTYRAGAMALMVTGCVGKNGGGLNHYVGQEKLAPVDSWGSL
                     AFAKDWVPVSRLQQAPLWHYINTCQYRYDGHHSNYNTTNKNKWTDKHVADTIFTSV"
BASE COUNT         2004 a          847 c          913 g         1791 t
ORIGIN      
        1 atttaccatt ccatcttttt ttgcactcat aataaatgca tcgcctagat taagcatttc
       61 ttgtgctatc tctttgtagg ttttattctc aaagccctcc tctctatgtt ttacataata
      121 actgttttct gcaattctgc aaccatcaat caccatcatt aaaccatatt tagtacaaat
      181 tgctctaaca tctttcatgt ttttcatgct tacaggctgg ccaccagacg aattgttagt
      241 tacagtcata ataattgcag caatgttttt accatgttgc tgaattagag tttctaattt
      301 ttctaaatca atattacctt taaaagcatg ataagattct acatcttctg cctctttaca
      361 aacaatatca taagcttcac tacctacata ttctatgttt gccctggttg tgtcaaaatg
      421 tgtattagaa ataaatactt ttcctttacc acctaaaata ctatacaaaa tacgttctgc
      481 tgctctacct tggtgtgtag gtaaaacata ttgcattccg gtaaggtttt gtacaacggt
      541 ttgtaaatgt tcccaacttc ttgcaccagc ataactttcg tctcccatca ttattcctcc
      601 ccactgtttg tcactcattg cacctgtacc actatcggtt aataaatcaa taaaaacatc
      661 gtccgacctt aataaaaatg gattataacc cgccttttct aaagcatttt gcctatattc
      721 ttctgtagta atacgcaagg gttcaaccat ctttatttta aatggttctg ttagtgttct
      781 cattttttta ctcatataaa ttgttataaa gggatgaaaa gagatagatt tttttgggtg
      841 gcaaagatag tagattaatt aaaaaagaca ccaaagtctt aatttaattt tataaacgga
      901 aagtataata ataaatttaa taaatattac acagtactac aatggttaat ttaataattg
      961 cttattaata tggtttctat ttttaagagt agaaacaata aagcatgcta atacaaacgg
     1021 aagtgtaagt tgaataattt gtatttgatt cataagcaaa ctaaaaatta gcgaaaggga
     1081 agaggctagc aaaacccaaa aaatagtaaa tagttttcta ttagcaaatg caatagcaca
     1141 taacaatgca ttgtagctaa atagcccagc attaacaaga tttaaaggaa tggaaagact
     1201 aattgcaatt aaaccagata actccgaaaa tgtaatagca taaatggcag ctataggaga
     1261 actaataaaa atagcaataa aaaaaactat accagatact gcattttctt gaaaaattac
     1321 ttgaccaaac cctttaaaaa tatagtcaaa atttaaattg tatgcagggc tacagccttc
     1381 gtttaatatt aaaagatttg gtgtaaatgt ttttataaaa aaaataatac tatatgtaac
     1441 agttacaaaa gataatgtgt aaacgggtaa ttttttttta ataaagaagt tgtgtaaaat
     1501 tgtggatagt acgcatgcaa ttattaaaat agaaatagat aagattttta tttttaagaa
     1561 tacaatacca gcaatgccta ctagtgcagc gttaaatcca taaagccctt tatttatatt
     1621 atttacatca tactttaata gtttggctgt gcttgtacca attattgttg caaaaaatat
     1681 tgtaatgccc attataaccg aattgtaaaa tattcctatt aaaaacaaca atcctgtaat
     1741 tgcattttct tgtaacataa tttggccaat accaacaaaa taagacctaa cagttgttct
     1801 aagagttata aacatataaa gttgtaattg cttacaatga agttactaaa acaatgaatt
     1861 taaactaaac tatattcagt tttagaattg taattttatt aattctaatt aagaaattgt
     1921 aaacattgtg ttttaaaaag gaatgtaaac tttagcaaag taggtatcct acattatact
     1981 aatttaaaaa ataaaaacat ttaatcaact aatttagtag agtaggcata ggcggtttct
     2041 gtttcaaaaa tttttatacc cacttttgaa atgttgtttt cctttaaaac ctgtttattt
     2101 tcatttatta tggtttctaa aaaaaaggca cataaatttt ctgcagtaga atcgtagggt
     2161 aataaaaaat acttccattg tttggcctca aatatttctt ttaactctat atcgttttta
     2221 gaaattatag ttgcatgatc tatttgttta accaaggggt ctaacatatt tttaatgtca
     2281 ttaaaatcaa taatcattcc gttttcattt ggctcccctt caaactcaac aatcatttta
     2341 taagaatggc catgtaattg tttacattta cctatatgcc aaggtagcct gtgtgcagcc
     2401 tcccacttaa atttttttgc tacaatcatt tatagttata ctattttaaa agaatatgca
     2461 aaattgtaag tttttttctt aaaacaatat gtaaactttt gtttttactg catatatgcc
     2521 ttctattatt atcaaaatat aagggtattt atttaaaaaa aatattttgg ttctatttcg
     2581 cttcgggata tatttgtctt tgattacgaa atcgggaaaa aattaaatgg aaatagtaca
     2641 atagcaagtt aatatcaaaa gccaggagat gtaacaattt tttaaagctg taaaaacgaa
     2701 cacagtatga aaatagttcc tcacttaatt tttgtaacaa taactttatt tgttatttcg
     2761 gctaaaaagc tacatgccca aaaagcagga aacgaaattt ttaaagaaac ctgctctgcc
     2821 tgccacactg ttggaaaagg aaaacttata ggcccagatt tagccaatgt tcacaaccac
     2881 ttaacattag attattttac aaaatttgta aagtcatctc aatcggtaat aaaatctgga
     2941 gataaatatg ccgattcaat ttttaatgct tatggtaaaa tggcaatgcc agatcatcct
     3001 caactttctg aagaacaaat aaaatctatt tatgattatc taaaagaagc tagtggaggt
     3061 ggtgcagaag aagcaatacc agaagaagaa ttattaccag gagatattga aagaggcagg
     3121 gctttatttg ttggtaactt aagattcact aactctggtg catcttgcaa ctcttgtcat
     3181 aacgtaaata ttgcaggcct tattacaggc ggtgctttag caaaagatct tacacaaagt
     3241 gttaccagat taactgaaac aggtgttgca ggcgttataa aaggattgcc ttatccacaa
     3301 atgaaagaaa cctatgcaat gcatcctatt acaccacaag aggttgcaga cttaactaca
     3361 tttttagtat atgttgatga aaaatcggca attaaaactg gtaattacac aggtaactat
     3421 atgcttttag gtggcggtgg tggtatagta tttttattaa gcctttactc atttttttgg
     3481 tttaaaagaa aaaggcaagc tgtaaacaaa tcggtattta gcagacaaat aaaatcggct
     3541 taaataaaaa ttaaggaata aaaaaatacc aaacctaacc ctaaaaaaaa taactttttc
     3601 ggcagcaaag tttaagaaac tatagaacta attaaataat tgaaagattt taaggtttaa
     3661 aaatattcat tcatctataa taataaaaac atgagctgga taaaagacat catctctcct
     3721 caaacacgca aatgggaaga attttataga aacagatttc aacatgatag aattgttaga
     3781 agtacacatg gggtaaattg tactggtggc tgctcttggg ctatacatgt aaaagaagga
     3841 attgtggtat ttgaaacaca acagttagat tatcctttaa tagatgataa acttccgcct
     3901 tacgaaccaa gaggttgtca acgaggcatt tcttattcgt ggtatttata tagtccatta
     3961 cgtataaaat atcctttgat aagaggtact ttattagatg cttttagaga agaaaaacaa
     4021 aaagtaaatg gagacccaat gaaagcctgg gagaatttac agaataataa agagcaaaga
     4081 aaaaaatacc aaaattcaag aggtaagggt ggctttagaa gaacaacatg ggatgaggtt
     4141 ttagaaatta tggctgcagc caatattcat actgctaaaa aatatggccc cgatagggtt
     4201 attgggtttt cacccattcc ggcaatgtct atgttaagct atgcatcggg tgccaggttt
     4261 ttacaattat ttggaggtgt aaatcttagc ttttacgatt ggtattgcga tttacctcca
     4321 gcctttcctg aaatatgggg cgagcaaaca gatgtttgcg aaagtgccga ttggtacaac
     4381 gcaaaaatga ttgctgatat gggtaccaat ttaaatatga ctagaacacc agattgccac
     4441 ttctttgcag aatctagaca taatggtacc aaaactgtag ttttctctcc tgattttagc
     4501 caattatgca aatatgcaga tcaatgggtt cctcttcatg ctggcagcga tggtgcattt
     4561 tggatgtcgg ttacacatgt aattttaaaa gaataccatt ttgaaaaaca aacaccatac
     4621 tttattgatt atgtaaaaag atataccgat agccccatgt tggtacatct aacaaaaaca
     4681 gaaaatggct ttactgctgg cagaatggtt agagcaaatg aattaagtaa atggaaaaat
     4741 atagaaaatg gagactggaa atttttatcg atagatgaag gcacaggaga atttgtagtt
     4801 cctaagggca ccatgggata tagatgggat aaagcaggtg gaaaatggaa tatgaaatat
     4861 gaatgtggcg aaacagataa aagttacaat ccgatattaa gttttattaa taataatgac
     4921 gatgttttag aagtaacatt tactgaattt ggtttatcta aaacagctga aagaggtgtt
     4981 cctgtaaaat ggcttgatac agaaaatgga agagtaccag ttaccacagt gtatgattta
     5041 accatggctc aatatggtgt agacaggggt ttaaaaggag attacccaac agactataat
     5101 aatccagatg ctgcatacac accagcctgg caagaaatat ttacaggcat tgattctaaa
     5161 actttattac aatttgcaag agagtgggcc gaaactgcta atacaacaga aggaaaatgt
     5221 atgatattag ttggtgctgg tgttaatcat tggtaccatc aaaacctaac ttatcgtgca
     5281 ggggcaatgg cattaatggt tactggttgt gtaggtaaaa atggaggagg tttaaatcac
     5341 tatgtggggc aagaaaaatt agcaccagta gattcttggg gttctttagc ttttgctaaa
     5401 gattgggtgc ctgtttcaag gttacaacaa gcaccacttt ggcattatat taatacttgc
     5461 caatataggt atgatggaca ccattcaaac tacaacacta ctaataaaaa taaatggaca
     5521 gataaacacg tggcagatac catatttaca tctgt
//
LOCUS       DAOQHP010000114         5544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8196_length_5544_cov_4.108581, whole genome shotgun sequence.
ACCESSION   DAOQHP010000114 DAOQHP010000000
VERSION     DAOQHP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_5544_cov_4.108581"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="rplC"
                     /locus_tag="PLY81_04935"
     CDS             complement(<1..495)
                     /gene="rplC"
                     /locus_tag="PLY81_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNF29603.1"
                     /translation="MKGIIGKKIGMTSIFSADGKQTACTIIEVAPNVVTQVKTIDTDG
                     YNALQLAIGDKKEKHATKAEINHFAKANTPAKQFTKEFRDYSLTKNLGETITIDIFEE
                     GDKVEVVGTTKGKGFQGVVKRHGFSGVGEGSHGQHDRQRAPGSIGGSSYPSRVFKGMR
                     MAGRM"
     gene            complement(795..1100)
                     /gene="rpsJ"
                     /locus_tag="PLY81_04940"
     CDS             complement(795..1100)
                     /gene="rpsJ"
                     /locus_tag="PLY81_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNF29604.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAERIVKTVRSTGAVVTGPIPLPTR
                     KRIFTVLRSPHVNKKSREQFQLCTHKRLLDIYTSTSSTVDALSKLDLPSGVEVEIKA"
     gene            complement(1336..1899)
                     /locus_tag="PLY81_04945"
     CDS             complement(1336..1899)
                     /locus_tag="PLY81_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29605.1"
                     /translation="MKINRSVLFTVAIIILTVVVKFICAPILSLSGISTVIAVALFAG
                     MSIHKKQNTFMLPLIALIVSDIIIEVLYQFNWFQFSGFYKGQILNYGLLLLSVLVGWA
                     IKGKSILSIGLGIFAASTIFFILSNLSVWYFGTMYTKDFSGLMLCYESALPFYKNQLI
                     STAIFLPIIMFAYNYFIEKKYRVAVSH"
     gene            complement(1975..3171)
                     /gene="manA"
                     /locus_tag="PLY81_04950"
     CDS             complement(1975..3171)
                     /gene="manA"
                     /locus_tag="PLY81_04950"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNF29606.1"
                     /translation="MNDKIFKLKGVVQHYAWGGFSFIPNLLKLKPTNQQPYAEYWLGA
                     HTSAPSVVVNKNISLLELINQNPEKIVGQEVVKQYNGLPYLFKILDVHEMLSIQVHPN
                     KEEAIKGFEKEEQAGIPINAINRNYKDKNHKPEVMIALSEFWLLHGFKEKSEIEQTLT
                     NHLELNLLLPTFKKEGIKGLYKIAMELPQEQVNTILKPIVKKSIFNKSQNLINKHNTE
                     WWIAKFYANNSTENLDRGIFSILLFNIVQLQKGEAIFQAAGIPHAYLEGQNVELMANS
                     DNVLRGGLTPKHIDVPELLKHTLFQSIQPSTIKPQINGNEKTYLCPVPDFSISIISLK
                     VGERYSSHTFSFEILLVIEGAIIIQGLENNIIKQGEAIGIVADTNYTFTATGDAIIFK
                     AFVPQP"
     gene            3205..3564
                     /locus_tag="PLY81_04955"
     CDS             3205..3564
                     /locus_tag="PLY81_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNF29607.1"
                     /translation="MKLNTKNIGNLGEDLSAKWFELKGYFILERNWRYKRSEVDIIAS
                     KENKLHFIEIKTRTNSNFGYPEESINQVKMNALKKAAVAYLELNTCWKEFQFDVLSVQ
                     LNKNKDHEFFLIEDVFF"
     gene            complement(3575..4051)
                     /locus_tag="PLY81_04960"
     CDS             complement(3575..4051)
                     /locus_tag="PLY81_04960"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNF29608.1"
                     /translation="MEDILYTDDYFMQQALKEANKAFNEDEVPIGAVVVVNNKIISKG
                     YNQTEKLNDCTAHAEMIALTSAFNFLGAKYIPQASLYVTVEPCLMCCGALYWSKLAKI
                     VYGASDEKSGGLRFFKNNDGEILTSKWPFHPKTKLIKGVLENECEMLMKTFFKLKR"
     gene            4117..5487
                     /locus_tag="PLY81_04965"
     CDS             4117..5487
                     /locus_tag="PLY81_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF29609.1"
                     /translation="MKEQLQNLRNYFNTGATKKYLFRIEQLKKISFALKKYEKEILIA
                     LHTDLGKCEAEAYASEIAIVQNEIKFFIKQLKRLMQPKEVSTNLMNLPGKSKIYYEPL
                     GIVLIIAPWNYPFQLAFNPLVGAIAAGNCVVVKPSEFANATSLIMEKIVKEIFSEDYI
                     YLVQGDGAQIVPTIIEEGSINHVFYTGSTSVGKLIYEMAAKKLIPVTLELGGKSPCVI
                     EDDANLKIAAKRVVIGKFINAGQTCIAPDYLLIHKKIKAKFLEILISTIQSFYTNNAA
                     LSKDYGKIINEKRFNTLISFLQQGKIIYGGKYDKESLYIEPTIMQEIPTNAPINHEEI
                     FGPILPIFDFETQSEAVEIIKQNATPLAFYIFTSSTTKEENWINAIPFGGGCINNTAY
                     HFTNHHLPFGGIGLSGIGSYHGKKSFYTFTHAKPVLKSATWFDPNIKYPPFKGKLRWL
                     KMILKL"
BASE COUNT         1930 a          848 c          809 g         1957 t
ORIGIN      
        1 catacggcct gccattctca ttcctttaaa aactctagaa ggataagaag aaccgcctat
       61 tgaacctggt gctctttgac gatcatgttg accgtggctt ccttcaccca caccactaaa
      121 tccatgacgc tttacaacac cttgaaagcc tttaccttta gttgtaccaa ctacttcaac
      181 cttatcacct tcttcaaaaa tgtctatagt aattgtttcg cctaggtttt tagttaaaga
      241 ataatcacga aattcttttg taaattgttt agctggagtg ttagctttag caaagtgatt
      301 aatttctgct tttgtagcat gtttttcttt tttatcacca attgcaagct gaagtgcatt
      361 gtaaccatct gtatcgatgg ttttaacttg tgtaacaaca ttaggagcaa cttctattat
      421 ggtacaagct gtttgcttac catcggcaga gaagatgcta gtcatcccaa ttttttttcc
      481 aataattcct ttcatcgttt tgcggtttgt gcccgctgag gttataaagg acattatgct
      541 gatgaaaagc ataattcaat ccttgtttgc taccgacctt ttcctttgtt tttcttaaat
      601 taagaaaggg gaagtaccga tagtaaacaa acctcgaagg gcttgtttat atgtttttcc
      661 taaatatttt gataataaaa tttttaggac ttttattttt cagagctctt ttcttcgtag
      721 tacgaattga gagataaata tttgttttat aagaactgtt cgtttattta gcgtaaaacg
      781 aaaaacgccg tttgttatgc cttaatttcc acctcaacac cacttggtaa atctaattta
      841 cttaatgcat ctacagtact tgaagtagaa gtataaatat ctaacaagcg tttatgtgtg
      901 cacaattgaa actgctcacg gctttttttg ttaacgtgtg gtgaacgtaa tactgtaaat
      961 attcttttac gtgtaggcaa aggaatagga ccggttacta cggctcctgt gctacgaact
     1021 gtttttacaa ttctttcagc agatttatct actaaattgt gatcgtagct ttgtaattta
     1081 attctaattc tttgagacat actaaaaccc aattttccgt tggggttgca aaagtagggg
     1141 ctttagagtt actgtgcaaa gtttttttga aaaaaaatca attttataca atttatttta
     1201 aaaaattatc tgtccaatag ataaaaataa tgctttaatt catccaaatt tattattaat
     1261 tgattaataa tcaatatatt aaaataaaaa aatcctgtaa taaattacag gattgaatga
     1321 atgaatattt tattactaat gtgatacggc tacacggtat ttcttttcta taaaatagtt
     1381 ataagcaaac ataattatag gcaagaagat agcagtagat attaattggt ttttataaaa
     1441 tggtaaagca ctttcataac aaagcattaa accactaaaa tcttttgtgt acatggttcc
     1501 aaaataccaa acacttaaat tagataagat gaaaaatatg gtagaagcag caaatatgcc
     1561 taaaccaatg gatagaatac ttttaccttt aattgcccaa ccaactagta cacttaataa
     1621 taataaaccg taattcaata tttgcccttt ataaaaaccc gaaaactgaa accaattaaa
     1681 ctggtacaat acttctatta taatatcact aacaattaat gctattaatg gcaacataaa
     1741 ggtgttttgt tttttatgaa tgctcatgcc agcaaacaat gctactgcaa taacggttga
     1801 aataccactt aaacttaata ttggggcaca aataaacttt actactacag ttaaaattat
     1861 aattgcaact gtaaataaaa cggaacgatt aattttcatg atagtgaaaa atttttacaa
     1921 aaataaggcg ttataacgtt tgtttcaaat gagatttgtt gaataaagaa aattttaagg
     1981 ttgaggtaca aatgctttaa aaataattgc atctcccgta gcagtaaaag tgtagtttgt
     2041 atcagcaaca attccaatgg cttctccttg ttttattatg ttgttttcta acccctgaat
     2101 tattatggca ccttcaatta ctaataatat ttcaaaagaa aaagtgtgag aagaatatct
     2161 ttcgcctact tttaatgaaa tgatgctaat agaaaaatct ggtaccgggc ataaataagt
     2221 tttttcgtta ccattaattt gaggttttat ggtagagggt tgaatagatt gaaataaagt
     2281 atgttttaat aactcaggta catctatatg tttgggagtt aatccacctc ttaatacatt
     2341 atcgctattg gccattaact ctacattttg tccttctaaa tatgcatgtg gtattccagc
     2401 tgcctgaaat attgcttctc ctttttgcag ttgaacaata ttaaaaagta gtatagagaa
     2461 tattcctctg tctaaatttt cagtactatt atttgcataa aattttgcaa tccaccactc
     2521 tgtattatgt ttattaatta aattttgaga tttattaaaa atagattttt ttactattgg
     2581 ttttaaaata gtatttacct gctcttgagg cagctccatt gcaattttat atagcccttt
     2641 tattccttct tttttaaagg ttggaagcaa taaatttaac tctaaatggt tggttagagt
     2701 ttgttcaatt tctgattttt ctttaaagcc atgtaacaac caaaactcac ttaatgcaat
     2761 cataacttct ggcttatgat ttttgtcttt ataatttctg ttaattgcat taataggtat
     2821 tcctgcctgc tcttcttttt caaaaccttt tatagcttct tctttgtttg ggtgaacctg
     2881 tatgcttaac atttcatgaa catctaaaat tttaaataag tagggtaacc cattatactg
     2941 tttaactact tcttgcccta caattttttc tggattttga ttaattaatt caagtaaaga
     3001 aatattttta ttaactacaa cagaaggggc tgaagtatgt gcacctagcc aatactctgc
     3061 ataaggttgt tgattagttg gttttaattt taataaatta ggaataaaag aaaaccctcc
     3121 ccatgcataa tgttgaacaa caccttttaa tttaaaaatt ttatcgttca tttgttttgt
     3181 ataactttat tctgctcaaa cataatgaaa ttaaacacaa aaaatatagg taatttaggc
     3241 gaagatttat cagcaaaatg gtttgagtta aaaggttatt ttatactgga aagaaattgg
     3301 aggtataaaa gaagtgaagt agatattatt gccagcaaag aaaataagct acattttatt
     3361 gaaattaaaa caagaaccaa cagcaatttt ggttatccgg aagaaagcat taaccaagtt
     3421 aaaatgaatg cattgaagaa agctgcagta gcctatttag aattaaatac ctgttggaaa
     3481 gaatttcaat ttgatgtact gtcggtacag ttaaataaaa acaaagacca tgagtttttt
     3541 ttaattgaag atgtattttt ttaaaaaatt attattacct ctttaattta aaaaaagttt
     3601 tcataagcat ttcacattcg ttttctaaaa ctccctttat caattttgtt ttaggatgaa
     3661 aaggccattt gcttgtaaga atttctccat cattattttt aaaaaatctt agtcctccac
     3721 ttttttcatc acttgcacca taaacaattt tagcaagttt actccaatac aaagcaccac
     3781 agcacattaa acaaggttca acagttacat ataaagatgc ctgtggaata tacttagcac
     3841 ctaaaaaatt aaatgcactt gttagtgcaa tcatttctgc atgggctgta caatcattta
     3901 gcttttctgt ttggttatac cctttactaa ttattttatt gtttacaact acaactgcac
     3961 caataggcac ttcatcttca ttaaaagcct tatttgcttc ctttaaagct tgttgcataa
     4021 aataatcgtc ggtatataaa atatcttcca ttaataatta caaatatgaa ctttatttta
     4081 aaatgtatga ttaacttgtg ttattatatt taatatatga aagaacaact acaaaattta
     4141 agaaattatt ttaatacagg ggctacaaaa aaatatctat ttagaataga acaattaaaa
     4201 aaaatttctt ttgcacttaa aaaatatgag aaggaaatat taatagccct tcatacagat
     4261 ttaggtaaat gtgaagcaga agcatacgcc tctgaaatag ctattgtaca aaatgaaatt
     4321 aagtttttta ttaagcaact aaaacgctta atgcaaccca aagaagttag caccaattta
     4381 atgaacttgc ccggaaaaag taaaatttat tacgaaccat taggcattgt tttaattatt
     4441 gctccctgga attatccatt tcaacttgct tttaaccctt tagttggagc aattgctgct
     4501 ggcaattgtg tagtagttaa acctagcgag tttgcaaatg ctacttcttt aattatggag
     4561 aaaatagtta aagaaatttt ttcggaagat tatatttatt tagtgcaagg tgatggtgca
     4621 caaattgtac ctactattat agaagaagga agtattaacc atgtttttta tacaggtagt
     4681 acaagtgtag gaaaattaat ttatgaaatg gcagccaaaa aattaatacc tgttacttta
     4741 gaacttggtg gaaaaagccc ttgtgtaatt gaagatgatg ccaatttaaa aattgctgct
     4801 aaaagagttg taataggaaa atttataaac gctgggcaaa cttgtatagc accagattac
     4861 cttttgattc ataaaaaaat aaaagctaaa tttttagaaa tattgatttc tactattcaa
     4921 agtttttata ccaataatgc agccttaagc aaagattatg gaaaaataat taacgaaaaa
     4981 aggttcaata ctttaataag ttttttacaa caaggaaaaa ttatttatgg aggaaagtat
     5041 gataaagaaa gcctttacat tgaaccaaca ataatgcaag aaatacccac taatgcccct
     5101 ataaaccatg aagaaatttt tggcccaata ctacccatat ttgattttga aacgcaatct
     5161 gaagctgtag aaataataaa gcagaatgct acccctttag cattttatat ttttacttct
     5221 tctacaacta aagaagagaa ttggataaat gcaattcctt ttggtggggg gtgtattaac
     5281 aatactgcat atcacttcac taaccatcat ttaccatttg gtggcattgg cctaagtggc
     5341 attggcagct atcatggaaa aaaatcattt tacacattta cacatgccaa acctgtttta
     5401 aaatctgcaa cctggtttga ccctaacata aaatatcctc catttaaagg aaaattaaga
     5461 tggttaaaaa tgattttaaa attataattt atttaaagta attatacctg tactactggt
     5521 ggatactaat ttttctgttt ctaa
//
LOCUS       DAOQHP010000115         5524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8243_length_5524_cov_3.991772, whole genome shotgun sequence.
ACCESSION   DAOQHP010000115 DAOQHP010000000
VERSION     DAOQHP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8243_length_5524_cov_3.991772"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLY81_04970"
     CDS             <1..216
                     /locus_tag="PLY81_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNF29610.1"
                     /translation="KFANLIIEKGSICVNGISLTCFNVTKNSFTVAIIPYTFTNTNMH
                     SITVGNYVNIEFDVLGKYINRAKELGS"
     gene            complement(213..1028)
                     /locus_tag="PLY81_04975"
     CDS             complement(213..1028)
                     /locus_tag="PLY81_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29611.1"
                     /translation="MGKLVFVYIIVISFYFNKSYANDTLLNLNIEQTIPGSFTNCYVD
                     NLGNIYLISATNQIKKLNSNLDSVAVFNDIKHFGTIDFIDVSNPLKILVFYKAFSTIT
                     ILDRFLSKKQTIDLRAINIQQAACIAQGYDNTIWVYDELDASIKKIDDFGKLLTTSTS
                     FRMLFDEAVLPTNIIDKDGLLYLYSKNFGWLIFDYYGALKTKVMEKNWSDVQVINKTL
                     FGHNHQELLWLQPTLPLLHKRHFNINLKDVQKLIQSNGYFYTLTSKGVTIYSY"
     gene            complement(1016..1771)
                     /locus_tag="PLY81_04980"
     CDS             complement(1016..1771)
                     /locus_tag="PLY81_04980"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF29612.1"
                     /translation="MTQATAKNIYFLSDFHLGAPNFSDSLLREKKIVAFLDSIKNNAT
                     EVFILGDMFDFWFEYKNTVPKGFTRILGKLAELTDSGIVIHFFVGNHDMWMKDYFKTE
                     LNIKVYFEPIITERFGKKLYIAHGDGLGPGDKGYKFLKKIFRNKICQWMFGILHPTWG
                     IGLANYFSRKSREKTGTADAHFLGEENEWLFIYSKEQLQKQPIDYFIFGHRHYPIKLA
                     IENSYYVNLGDWINNFTYVVLNNETLELKKWEN"
     gene            complement(1771..2202)
                     /locus_tag="PLY81_04985"
     CDS             complement(1771..2202)
                     /locus_tag="PLY81_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNF29613.1"
                     /translation="MQTVFNLFVYGSLRKGFKNPAYNYVSRYFSLVSNNAKAKGLLFN
                     MGNFPVAKPTANNKFITGELYTIINKDEFSFAIAQLDDYEGVYAEENEKPLYYREKTI
                     VYFENVATEAWVYWFNGEVNGKQIIESGDVLQFFSEQQKGS"
     gene            complement(2242..3057)
                     /locus_tag="PLY81_04990"
     CDS             complement(2242..3057)
                     /locus_tag="PLY81_04990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNF29614.1"
                     /translation="MVFITANFAKNNNLLPTSNTPNISLPTIGNTFIELAEIDSTNTY
                     AMKQIQAKVAEHGTAYFAHNQTNGKGTRGKSWHSETGNNIILSVILDINWLSVRNQFL
                     LSAWAALSVFDFLSKYVQNGIKIKWTNDIYIDNSKIAGILIENKVKGNKLQWAVVGIG
                     ININQINFDSTLSKATSLALVTNKKYDALLLSKELCFCLEKRFQSLINKDANGLINDY
                     NKNLFKLNEVVKLKKDNLLFNCTIKKVTKTGFLVVNNGIEQQLAFGEVEWILS"
     gene            3110..3517
                     /gene="rsfS"
                     /locus_tag="PLY81_04995"
     CDS             3110..3517
                     /gene="rsfS"
                     /locus_tag="PLY81_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNF29615.1"
                     /translation="MANLSVLNTRKKSTVVKLTRNSKIFKSIINAIEDKKGEHIVSLD
                     LRKIPEASADFFIICQASSTTQLKAICEFIVEEVKLKCNETPNKQEGKQALQWILVDY
                     INIVVHIMYPEARNFYKLEEMWNDAGILKHETN"
     gene            3589..>5524
                     /gene="ftsH"
                     /locus_tag="PLY81_05000"
     CDS             3589..>5524
                     /gene="ftsH"
                     /locus_tag="PLY81_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNF29616.1"
                     /translation="MDEQKQKNLRPNLNTGGNKSKKSPKFSIYWIYAAIIILLLSYRI
                     FDMGTPETVSTTSLEFKNEMLAKGDVSKIDQVDNAGRKSIKVYLIADSLDKPFYKEKF
                     KNATIKPKEVAKKGLPLFEFKVSDFKNFDDELSKFYSENKNIQEVPKNYKQEGEFWGP
                     IANIVLTLIIVIGSWVLLMRKMGGGSGSGGGAGGIFSIGKSKAQLFEKGTKVNINFGD
                     VAGLDEAKVEVMEIVDFLKNPKKYTALGGKIPKGALLVGPPGTGKTLLAKAMAGEAQV
                     PFFSLSGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGKNVMMSND
                     ERENTLNQLLVEMDGFAGDAGIIILAATNRPDVLDSALLRPGRFDRQITIDKPDVKGR
                     EAIFKVHLQPIKVSETLDVHKLAEQTPGFAGADIANVCNEAALIAARKNKAAVDMSDF
                     QDAIDRVIGGLEKKNKIIAPHEKEIIAYHEAGHAICGWFLEHAYPLLKVTIVPRGSAA
                     LGYAQYTPKEQHLYNTDQLMDQICMTLGGRAAEEIFFNKISTGAANDLQQITKIAYSM
                     VTVYGMNSKVGNISFYDPGQENAFQKPYSEETGKIIDEEVRKLIDTAYIRTKELLTEK
                     KEQVEKLAKELLDKEVLHQSD"
BASE COUNT         2026 a          837 c          832 g         1829 t
ORIGIN      
        1 aaatttgcta atttaataat tgaaaaaggc tctatttgtg taaatggtat tagccttaca
       61 tgttttaatg ttactaaaaa tagttttact gttgctatta taccttatac ttttaccaat
      121 acaaatatgc attcaattac tgttggcaat tatgtaaata ttgagtttga tgtattaggt
      181 aagtatatta acagagctaa agaattaggc agttaataac tataaattgt aactcctttg
      241 gatgttaagg tataaaaata accattactt tgaattagct tttgtacgtc ttttaaatta
      301 atattaaagt gccgtttatg tagtaatggc aaggttggtt gcaaccacaa aagctcttga
      361 tggttatgtc caaacaaagt tttattgatt acctgtacat ctgaccaatt tttttccatt
      421 actttagttt ttaaagcacc ataataatca aaaattaacc agccaaagtt tttgctatac
      481 aagtataaca aaccatcttt atctattatg ttggtaggta aaacagcttc atcaaacaac
      541 attctaaagc tggtagatgt agttagtaat ttcccaaaat catcaatttt ttttatagag
      601 gcatctaact catcataaac ccaaatagta ttatcataac cttgtgcaat gcatgcagct
      661 tgttgaatat ttattgctct taagtcgata gtttgttttt tgcttagaaa tctatctaaa
      721 atagttattg tagaaaatgc tttataaaat accaatattt tcaaagggtt cgaaacatca
      781 ataaaatcaa tggttccaaa atgtttaata tcattaaaaa cagcaacact atccaaattg
      841 gagtttagct tttttatttg attggtggca gaaattaaat aaatgtttcc taaattatct
      901 acataacaat tcgtaaaact gccaggtatt gtttgttcaa tatttaaatt taataatgta
      961 tcgtttgcat aacttttatt aaagtaaaaa ctaataacaa ttatataaac aaaaactagt
     1021 tttcccattt tttcagttca agagtttcat tatttaacac tacataggta aagttattaa
     1081 tccaatctcc taggtttaca tagtagctat tttctatagc aagttttatt ggataatgtc
     1141 tatgcccaaa aataaaataa tctattggct gtttttgtaa ttgctcttta ctataaataa
     1201 ataaccattc attttcttct cctaaaaaat gagcatctgc agtacctgtt ttttctctac
     1261 tttttctact aaaataattt gctaacccta tgccccatgt gggatgcaaa ataccaaaca
     1321 tccattggca aattttattt ctaaatattt tttttaaaaa tttatagccc ttatctcctg
     1381 gccctaagcc atcgccatga gctatatata attttttacc aaatcgttct gtaataatag
     1441 gttcaaaata tactttaatg tttagttcgg ttttaaagta atctttcatc cacatatcgt
     1501 ggttgcctac aaaaaaatgt attacaatgc cactatctgt tagctcagct aactttccta
     1561 aaattcttgt aaagcctttt ggtacagtat ttttatactc aaaccaaaaa tcaaacatat
     1621 cgcccaatat aaaaacttca gttgcattat ttttaatgct atctaaaaaa gcaactatct
     1681 ttttctctct taaaaggctg tcagaaaaat ttggggctcc taaatgaaaa tcggataaga
     1741 agtaaatatt tttagcagtt gcttgtgtca ttaactgcct ttttgttgtt cagaaaaaaa
     1801 ttgaagtaca tcgccacttt caataatttg ttttccgttt acttctccat taaaccaata
     1861 aacccatgct tctgtagcta cattttcaaa gtaaacaata gtcttttctc tatagtataa
     1921 aggcttttca ttttcttctg cataaacacc ttcataatca tctaactgag caatggcaaa
     1981 actaaattca tctttattaa ttattgtata aagttctcct gtaataaatt tattattggc
     2041 tgtgggcttg gctacaggaa aattgcccat attaaacaac aatccttttg cttttgcatt
     2101 atttgaaact aatgaaaaat acctgctcac ataattgtaa gccggatttt taaacccttt
     2161 tcttaaggaa ccatacacaa ataaattaaa aactgtttgc ataatgctaa gatacaagca
     2221 gtaattatat aatacaaatt actaactcaa aatccactct acttctccaa aagcaagttg
     2281 ttgctcaata ccattgttta caactaaaaa gccagtttta gtaacttttt taatagtgca
     2341 attaaataat aagttgtctt ttttaagttt tacaacttcg tttagcttaa ataaattttt
     2401 attataatca tttattaacc catttgcatc cttgtttatt aatgattgaa accttttctc
     2461 caaacaaaaa cacaattctt tagaaagtaa taaagcatca tactttttat tagttactaa
     2521 tgccagcgat gtagcttttg atagagtaga atcaaaattg atttgattaa tgttaattcc
     2581 aatgccaaca acagcccatt gtagcttatt gcctttaact ttattttcaa ttaaaatgcc
     2641 tgctatttta ctattatcta tataaatgtc attagtccat ttaattttta tgccattctg
     2701 tacatattta ctcaaaaaat caaaaacaga taatgcagcc caagcactta ataaaaactg
     2761 attacgtaca ctaagccaat taatatctaa tataaccgat agtataatgt tattgcctgt
     2821 ttcagaatgc cagcttttac ccctagttcc tttaccattt gtttgattat gagcaaaata
     2881 ggctgtacca tgttctgcca cttttgcctg aatttgtttc atggcataag tattggtact
     2941 atcaatttct gctaattcaa taaaagtatt accaatagtt ggtaaagaaa tgttaggagt
     3001 gtttgatgtt ggcaaaagat tgttattttt ggcaaaatta gcagtaataa aaaccatttg
     3061 gttttataca tgcaacataa aaattaatta actataataa tattcatttt tggcaaatct
     3121 ttctgtttta aatacccgca aaaaaagtac tgttgtaaaa cttaccagaa attctaaaat
     3181 tttcaaatcc ataattaatg caattgaaga taaaaaagga gaacatattg taagtttaga
     3241 tttaagaaaa ataccagaag catcggcaga tttctttatc atttgccagg cttctagtac
     3301 tactcaatta aaagcaattt gcgaatttat tgtagaagaa gtgaagttaa aatgcaatga
     3361 aacacctaat aagcaagagg gtaagcaagc attacaatgg attttagttg attatataaa
     3421 tatagttgta cacattatgt atcctgaagc aagaaacttt tacaaattag aagaaatgtg
     3481 gaatgatgca ggaatattaa aacacgaaac caattaattt aattacaaaa gccaagaagg
     3541 tcaaagaaaa aaacacaaaa ttcaacgacc ataaactatt atcaacctat ggatgagcaa
     3601 aaacaaaaaa acttaagacc taatttaaat acaggaggaa ataaatcaaa gaaaagccca
     3661 aagttttcga tttattggat atacgctgcc attattattc tattattaag ttatagaata
     3721 tttgatatgg gtacacctga aactgtttct accacatcat tagaatttaa aaatgaaatg
     3781 cttgccaaag gcgatgtttc aaaaatagac caagtagata atgctggaag aaaaagcata
     3841 aaagtatatt taatagcaga tagcttagat aaaccttttt ataaagaaaa atttaaaaat
     3901 gcaactataa aaccaaaaga agttgctaaa aaagggctac cattatttga gtttaaagta
     3961 tctgacttta aaaattttga tgatgaatta tccaaatttt actctgaaaa taaaaatatt
     4021 caagaggttc caaagaatta taaacaagag ggagaatttt ggggacccat tgcaaatatt
     4081 gttctaacac ttattatagt aattggctcc tgggttttat taatgcgtaa aatgggtgga
     4141 ggctcaggca gtggtggtgg tgctggtggt atttttagta taggaaaaag taaagcccag
     4201 ctatttgaaa aaggtactaa agtaaacatc aattttggtg atgtagccgg tttagatgaa
     4261 gccaaagtag aagtaatgga aattgttgac ttcctaaaaa accctaaaaa atacacagca
     4321 ttaggaggca aaatacctaa aggagcttta ttagttggcc ctccaggaac aggtaaaacc
     4381 ttattagcaa aagctatggc tggcgaagcc caagttccat tctttagctt aagcggtagc
     4441 gattttgtgg aaatgtttgt aggtgttggt gcaagccgtg taagagattt atttaaacaa
     4501 gcccgtgaaa aagcaccatg tattattttt attgatgaaa tagatgctat aggcagagca
     4561 agagggaaaa atgtaatgat gagtaatgac gaaagagaaa acacccttaa ccaattactt
     4621 gtagaaatgg atggctttgc aggagacgct ggtattatta ttcttgctgc aaccaacagg
     4681 ccagatgttt tagatagtgc tttattaaga ccaggcaggt ttgatagaca aataactatt
     4741 gataaaccag atgttaaagg cagagaagca atttttaaag tacacctaca acctattaag
     4801 gtttctgaaa cattagatgt acataagctt gccgaacaaa cacctggttt tgcaggtgcc
     4861 gacattgcaa atgtttgcaa tgaagctgct ttaatagctg ccagaaaaaa taaagcagca
     4921 gttgatatga gcgattttca agatgctatt gatagagtaa ttggcggact tgaaaagaaa
     4981 aataaaataa ttgctccaca cgaaaaagaa attattgcat accacgaagc tggtcatgct
     5041 atatgtggtt ggtttttaga gcatgcttac cctttattaa aagtaacaat tgtaccaaga
     5101 ggttctgcag cattaggata tgcccaatat acccctaaag aacaacactt atataataca
     5161 gatcaattga tggatcagat atgtatgact ttgggcggta gagctgcaga agaaattttc
     5221 tttaacaaaa tatctacagg tgcagcgaac gatttacagc aaattactaa aattgcttac
     5281 agtatggtta ctgtatatgg tatgaacagt aaagtaggta atataagttt ttacgaccca
     5341 ggacaagaga atgcttttca aaaaccttat agtgaagaaa caggtaaaat aatagatgaa
     5401 gaagtaagaa aactaattga tactgcatac atacgcacaa aagaattatt aacagagaaa
     5461 aaagaacaag tagaaaaatt agcaaaagaa ttattagata aagaagtatt gcaccaaagc
     5521 gatg
//
LOCUS       DAOQHP010000116         5487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8333_length_5487_cov_3.754050, whole genome shotgun sequence.
ACCESSION   DAOQHP010000116 DAOQHP010000000
VERSION     DAOQHP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8333_length_5487_cov_3.754050"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..484
                     /locus_tag="PLY81_05005"
     CDS             68..484
                     /locus_tag="PLY81_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29617.1"
                     /translation="MKKVLVFLFSIILFTSCSKQDNFIEPTNSTTGNQLFYRDAHLAI
                     QSFTLSGTNSNSIQIDFSTLYEKNISKIEIMSGETPNYLCAVYTVNLTINSAQLKNYS
                     VTDSKTSSSTAYYMIKYTLNNGDFGFTNVIKYQRNK"
     gene            complement(552..1544)
                     /locus_tag="PLY81_05010"
     CDS             complement(552..1544)
                     /locus_tag="PLY81_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29618.1"
                     /translation="MSLQYYNPFNNLYFNNNTCFLTGEEIEEQQNYITVFPEWVLDRF
                     LLHDKKFTLMDNVTSFKYNQMVLPCSIKVKNAYNLLDQEIKAAFENGYQSVQEIDSQK
                     LFTWMGVFVYCVLYQDLLIEKEKKQKQNAEFDISPTLKNRFALFHLMLQSLVAPISFG
                     AIKPWGITIVKLKYSKDIFNYRDDTVNLMFTLGMNGFGIIATLQDNGIVMQKEQALLH
                     KINQATLHPIQFEELKARFIYNNYLLQFKPKYIIKQHADCLDINAVEFKANDISTLFA
                     NWNEKTFADVLSEYWKPWGLKKNEIYTFPNAPLSFLENENTYNFIEPDTIDLPY"
     gene            complement(1598..1900)
                     /locus_tag="PLY81_05015"
     CDS             complement(1598..1900)
                     /locus_tag="PLY81_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNF29619.1"
                     /translation="MSELIPANIVIADRTYRLKIDADDEELVRKTAKLINDKILEFKD
                     SLAGKDMQDYVSMVLLWFATEQNKTGKAMISLQTTEQKLLAFEKQIDKAINSINTI"
     gene            complement(1910..2164)
                     /locus_tag="PLY81_05020"
     CDS             complement(1910..2164)
                     /locus_tag="PLY81_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29620.1"
                     /translation="MDSTIDQQLSVLQNKLQLLLEKCHALKKENEQLKKQIEKNKQSQ
                     ENSYKNLIALKTNNPEELEKRIDKYLQEIDRCLLLLNVEA"
     gene            complement(2217..4643)
                     /gene="pheT"
                     /locus_tag="PLY81_05025"
     CDS             complement(2217..4643)
                     /gene="pheT"
                     /locus_tag="PLY81_05025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNF29621.1"
                     /translation="MTISYNWLSEYLPIKIEPEKLSKILTSVGLEVEFMEPYQSIKGA
                     LEGLVIGEVLSCEQHPNADKLKITTVNINQEQPLQIVCGASNVAIAQKVVVATVGTTI
                     YPLSGDPITMKVAKIRGVESYGMICAEDEIGLSANHNGIIVLPNDVEVGNAAAQYFNP
                     YNDIIFEIGLTPNRMDAMSHYGVAKDVCAYLSHHLKEEAKPKNIFSVPFKTDNNNIPI
                     QVTIENIDACPRYAGVSISGVSIKESPIWLKNKLSAIGLKPINNIVDITNYILHETGQ
                     PLHAFDVNAITNNQIIIKNLDANTSFITLDGIERKLSNNDLMICNGANEPMCIAGVFG
                     GFKSGITPSTTNIFVECACFNASSIRKSSIHHSLRTDAATRFEKGVDIGNTINVLQRA
                     IALIKEIAGGTVSCEIIDVYPVKKEKAEVSLKNHYLKKLSGKNYHPDAVKRILTSLGF
                     EIVKEGMDEIRLAVPFSKPDITLAADIVEEIVRIDGLDNIEIPSSINITPQVTFLETK
                     EGLREKIANLLTGLGFCEILTNSITNSKYFTNNELNNTVKMLNNLSAELDILKPNMLP
                     TGLEVLVFNINRKNCNLQLFEFGKTYCVNSDGNYSEEEHVTLYLTGLESENNWNQKAK
                     TIGFYETKGIANAILKLLNIQDINFTEDASNASFINIATNNTLLGNIQIISKNTLDAF
                     DIKQAVFCVDFNFSALLNEVEKNKIVYKEVSKFPVVQRDLAMVVSSKTTYNQVEQVIK
                     KLKFNTLQSTRLFDVFMSDKLGADKKSMAVNFTFSDNEKTLTDKDIDGMMNKLIFHFE
                     KELAAEIRKQ"
     gene            complement(4760..5275)
                     /gene="rimM"
                     /locus_tag="PLY81_05030"
     CDS             complement(4760..5275)
                     /gene="rimM"
                     /locus_tag="PLY81_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNF29622.1"
                     /translation="MNNYIHIGKIVATFGVNGEVILNHSLAKKAGFKNITVLFVELIK
                     GNKIPYFLVHAKAKTNNETILLFEDINNKEAAHKLIGKPIWLNEEDFRKQAGKQAPIG
                     LLGYNVVENKNILGSVLEVIEQPHQVLLGILYKEKDAYIPLHQETLIKINHDKKEIIV
                     TLPDGLLDIYS"
BASE COUNT         1769 a          851 c          799 g         2068 t
ORIGIN      
        1 tttcaatttt tgcccgatta aagatataaa ttctttaaaa attttaatca aattataatt
       61 tcaggaaatg aaaaaggtac tagttttttt attctctatt attttattta cttcttgcag
      121 taaacaagat aattttattg aaccaacaaa ttcaactaca ggcaatcagt tgttttatag
      181 agatgctcat cttgccattc aatcatttac acttagtgga acaaatagta attctattca
      241 aatagatttt tctactttat acgaaaaaaa tattagtaaa atagaaatta tgagtggcga
      301 gacgcccaac tacttatgtg ctgtttatac tgtaaactta acaattaaca gtgcacagtt
      361 aaaaaattac agtgttacag attctaaaac aagcagctca actgcttatt atatgataaa
      421 atatacgttg aacaatggag actttggttt taccaatgta ataaaatatc aacgtaacaa
      481 ataagtgttt attcgtaccc aaaaagttta atccgccaac ctacacttaa acccagtttt
      541 actgggtttt tttaatatgg taagtcaatt gtgtcaggct caataaagtt gtaagtgttt
      601 tcgttttcta aaaaacttaa aggagcgttg ggaaaagtat aaatttcgtt tttctttaag
      661 ccccaaggtt tccaatattc acttaaaaca tctgcaaagg ttttttcgtt ccagttggca
      721 aataaagtag aaatatcatt tgccttaaac tcaacagcat taatgtctag gcaatcggca
      781 tgctgtttta taatgtattt tggtttgaat tgcagtaaat aattattata aataaatctt
      841 gcttttagtt cttcgaattg aattggatgt agggttgctt gattaatttt atgtagtaat
      901 gcctgctctt tttgcattac aataccatta tcttgtaggg ttgcaataat accaaaacca
      961 ttcatgccaa gtgtaaacat taggttaact gtatcatctc tataattaaa aatatctttt
     1021 gagtatttta gttttacaat agttatgccc catggtttta tagcaccaaa acttataggt
     1081 gctactaatg attgcaacat taaatgaaaa agggcaaacc tgttttttaa agttggcgaa
     1141 atatcaaatt ctgcattctg cttttgtttt ttttcttttt caattaataa atcttggtat
     1201 aaaacgcagt aaacaaaaac tcccatccat gtaaataact tttgactatc tatttcttgc
     1261 acagactggt agccgttttc gaaagcagcc tttatttctt ggtctaataa attatatgca
     1321 ttttttactt ttatggaaca gggtaacacc atttggttgt atttaaatga ggttacatta
     1381 tccattaggg taaatttttt atcgtgtaat agaaagcgat ctaaaaccca ttcaggaaaa
     1441 acagtaatat aattttgttg ctcctctatt tcttctcctg ttaaaaaaca agtattattg
     1501 ttaaaataga gattattaaa gggattgtaa tattgtaaag acattatttt gttttaacag
     1561 atttaaaaga gagaaaaatt aaaacaaaag agatagatta aattgtattg atgctgttaa
     1621 tggctttatc aatttgcttt tcaaaggcca acagtttttg ctcggttgtt tgaagagaaa
     1681 tcattgcttt gccggtttta ttttgttctg ttgcaaacca aagcaaaacc atactaacat
     1741 aatcttgcat gtctttacct gcaagagagt ctttaaactc tagaatttta tcattaatta
     1801 gtttagctgt ttttcgtacc agctcttcat catcggcatc aatttttaaa cgataggttc
     1861 tatcggcaat tacaatattg gcaggaatta attcagacat ttttaaaaat tatgcttcta
     1921 catttaataa taataaacac ctatctattt cttgtaaata tttatctatt cttttttcta
     1981 actcttccgg attatttgtt ttaagggcta ttaagttttt atacgaattt tcttgacttt
     2041 gtttattttt ttctatttgt ttttttagtt gttcgttttc tttttttaaa gcatgacact
     2101 tttccaacaa aagttgaagc ttattttgta atacagataa ttgttgatct attgttgaat
     2161 ccatttttac aaatctagtt aattaaagtt ttcaatactt ttattatagt tggtgtttat
     2221 tgttttctta tttctgctgc caattctttt tcaaaatgga aaattaattt attcatcatg
     2281 ccatcaatat ctttatcggt taatgttttt tcattatcgc taaaggtaaa gtttacagcc
     2341 attgattttt tatctgcacc aagtttatcg ctcataaaaa catcaaacaa acgagtactt
     2401 tgaagtgtat taaattttaa tttttttatt acttgttcta cttgattata ggtagttttt
     2461 gaacttacaa ccattgccaa atctcgttgt acaacaggaa atttgcttac ctctttgtaa
     2521 actattttgt ttttttcaac ttcatttaat aaggcagaaa aattaaaatc aacacaaaaa
     2581 accgcttgct ttatatcaaa tgcatccagt gtatttttag atattatttg tatattacct
     2641 aacagggtat tattggtagc aatatttata aaagatgcat tgcttgcatc ttcggtaaaa
     2701 ttaatatcct gtatattcaa cagtttaagt attgcattgg caattccctt ggtttcataa
     2761 aaaccaatag ttttggcttt ttggttccag ttattttcac tttctaagcc tgtaaggtat
     2821 agggttacat gttcttcttc cgaataattg ccatcgctgt ttacacaata tgttttacca
     2881 aattcaaaca actgtaggtt acaatttttt ctattaatat taaagaccaa cacttctaat
     2941 cctgtaggta gcatattggg ttttaagata tccaactcgg cacttaagtt gttaagcatt
     3001 ttaacggtgt tgttcaactc attgtttgta aaatacttac tatttgtaat ggagttggtt
     3061 aaaatttcgc aaaagccaag gccggtaaga agattagcaa ttttttctct caacccttct
     3121 tttgtttcta aaaaggttac ttgtggggtt atgtttatgg aagatggaat ttcaatatta
     3181 tctaagccat caattcttac aatttcttca acaatatctg cagcaagggt tatgtctggt
     3241 ttgctaaaag gtacagccag tctaatttca tccattcctt ccttaactat ttcaaagccc
     3301 aggcttgtta aaattctttt aactgcatcg gggtgataat tttttccgct tagttttttt
     3361 aagtaatggt tttttagaga aacctcagcc ttttcttttt taacagggta aacatctata
     3421 atttcacaag aaaccgtacc gcctgcaatt tcttttatta aagctatagc cctttgtaaa
     3481 acattaattg tattgccaat atcaacacct ttttcaaatc tagttgcagc atctgttcta
     3541 aggctatggt gtattgaaga tttgcggata gagcttgcat taaagcaagc acattctaca
     3601 aaaatatttg ttgtagaggg tgttattcca cttttaaatc ctccaaaaac accagcaatg
     3661 cacattggtt cattggctcc attacaaatc attaaatcat tgttgcttaa ttttctttct
     3721 ataccatcta aggttataaa gcttgtatta gcatctaagt tttttattat aatttggttg
     3781 tttgtaattg cattaacatc aaaagcatgt aagggctggc cggtttcgtg taaaatataa
     3841 ttagtaatat caacaatatt gttaattggt ttaaggccaa tagcagataa tttatttttt
     3901 aaccaaatgg gactttcttt aatagaaacg ccactaatac taacacctgc atatcttggg
     3961 caggcatcta tattttcaat agttacctga atgggtatgt tattattatc tgttttaaat
     4021 ggtacagaga aaatgttttt gggctttgcc tcttctttta aatgatgaga taaatatgca
     4081 caaacatctt tggctacacc ataatggctc attgcatcca ttctattagg ggttaagcca
     4141 atttcaaaaa taatatcatt ataagggtta aaatattgag cagcagcgtt gccaacttca
     4201 acatcattgg gtaatactat aatgccatta tggttggcag ataaaccaat ttcatcttcg
     4261 gcacaaatca ttccataact ttctacacca cgaattttag ctactttcat ggttattggg
     4321 tctccactaa ggggataaat ggttgtacca acagtggcta caacaacctt ttgggctatt
     4381 gcaacattag atgccccaca aacaatttgc aggggttgct cttgattaat atttacagtg
     4441 gtaattttta gtttatcggc attggggtgt tgttcgcaac ttaaaacttc tccaattacc
     4501 aaaccctcta atgcaccttt aatactttgg tagggttcca taaactccac ttctaaacct
     4561 acagaagtta atatttttga aagtttttct ggttcaattt ttatgggtaa atattcactt
     4621 agccaattat agcttattgt cataatttat ttgcttatac agctagcgaa gatactattt
     4681 aaagaaatgt ataaaacaca ttaaatatat tttttaaaga atagaattta gtaaggaaag
     4741 gaaagtaaat aactggtttt taggaataaa tatctaacaa tccatctggc agtgtaacaa
     4801 taatttcttt cttatcgtga ttaattttta ttaaggtttc ctgatgaagg ggaatataag
     4861 catctttttc tttgtaaaga atacccaata aaacctgatg gggttgttct ataacttcta
     4921 aaacagagcc taaaatattt ttattttcaa caacattata acccaataag ccaatagggg
     4981 cctgctttcc agcttgcttt ctaaaatctt cctcgtttag ccaaattggt ttgcctataa
     5041 gcttatgggc agcttcttta ttgtttatgt cttcaaacaa taaaatggtt tcgttattgg
     5101 ttttggcttt tgcatgaact aaaaagtagg ggattttatt gcctttaatt agttctacaa
     5161 aaagtacagt tatattttta aagcctgctt ttttagctaa agagtgattt aaaatcacct
     5221 ctccatttac accaaaagtg gccacaattt taccaatatg gatataatta ttcataaaac
     5281 aaactgcaaa attatttaat ttgaatatac taccaattaa actataaaac agctactttg
     5341 attccttgaa aattaatttt taatacataa aaaaagataa taaaaagagc ggttttatat
     5401 gtacattcgc aagcattttg aaattaataa aaatcaagta gcacaagcat tatgagcatg
     5461 gaacaaaagt caaattatgg tgccgat
//
LOCUS       DAOQHP010000117         5453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8415_length_5453_cov_4.095961, whole genome shotgun sequence.
ACCESSION   DAOQHP010000117 DAOQHP010000000
VERSION     DAOQHP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_5453_cov_4.095961"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLY81_05035"
     CDS             <1..390
                     /locus_tag="PLY81_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29623.1"
                     /translation="NIKLKQTALESYYNLTQNKEVLKAFSTSNLEEMKATAEILNNKE
                     NPNPYFSKEEFLNKLQLNSIVNQYQLQYLCRFAIGLENENKLNNNFIAVNKTTALEYI
                     LKVKANNNQKILLDIIGLSVLFRLQQE"
     gene            496..1326
                     /locus_tag="PLY81_05040"
     CDS             496..1326
                     /locus_tag="PLY81_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF29624.1"
                     /translation="MIETILLPSDFSATAVNAGIYAIELAQQVGAKKIVVYHTYDAAS
                     VSDPMVSYTQKIVTEPFRQKSAAQLNEFIDKLNAKNKSSIVIEAFHSYADLKDALNEL
                     IASTGAQLIVMGITGGGVFKETFIGSNSVNVAKSTNIPVIIVPTETSFSPISNILLLS
                     DLNNVADTTPTEAIKNILDASKAKLHVLNVTDNTEKAEASSDKQQLGKLFEAYNPTFN
                     FMSNPDFVGAVDWFVKEKGIDLVIVIPKKHGLFETLFKFNHTKTLAFHGKVPLMAVKK
                     "
     gene            complement(1379..1939)
                     /locus_tag="PLY81_05045"
     CDS             complement(1379..1939)
                     /locus_tag="PLY81_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNF29625.1"
                     /translation="MKKTFIVLTTMAMYLLTACSGEQTTTGSESTTQTTDAVGQSGVV
                     DDQSAKNIVQTAAGSKDHTTLVAAVKAAGLLDALSNAGPFTVFAPTNAAFDKLPAGTV
                     EDLLKPENKGKLENALGYHTYVGSIKAEMMQDGQELNMVFEGNVTITKKDGKTFVNGS
                     EIVASIPTSNGMIHVINDVLMPPAKK"
     gene            complement(2062..2769)
                     /locus_tag="PLY81_05050"
     CDS             complement(2062..2769)
                     /locus_tag="PLY81_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29626.1"
                     /translation="MIENKACILLVEDEEGLNETLKLNLEMEGYDVTSAYAGDEALTE
                     LSKAHFDLMILDIMLPEIDGITVAQTVRLSNDELPILMLSAKNSGADRVLGLKKGADD
                     YLTKPFNLEELLLRVEKLIQKNKKIYDKESTLGDTYSFGNNLIDFKSLDAITYDGKKI
                     KLSKKETNLLKLLIENKNEVVPREKILLVVWGYNVFPTTRTVDNFILMFRKHFEENSK
                     QSKHFHSVRGIGYKFTD"
     gene            complement(2773..3747)
                     /locus_tag="PLY81_05055"
     CDS             complement(2773..3747)
                     /locus_tag="PLY81_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF29627.1"
                     /translation="MKSFFHKKLALITTVYWFLLLYMIAALLFWFIKLNHHASDTAEM
                     QIALLQTSDSLYKEKISTIEATKKRKIAQYLGEGTTFLAVILIGAVFVYRATRKQIRL
                     SVQQQNFMMAVTHELKTPIAITHLNLETLQKRKLDEEKQQKLISNTIQEANRLNSLCN
                     NILVAAQLDGGAYYSNTYELNLSDLTETCIEDFAIRYPQRKLEADIQQQLFINGEALL
                     LQMLVNNLIENALKYSPKEMPVTIRLYTSNKQTFLDVLDKGCGVKPQDRKKIFDKFYR
                     SGSESTRTTKGTGLGLYLCKKIMKSHNGNITMTNNHPQGSIFTAVFNM"
     gene            complement(3747..4202)
                     /locus_tag="PLY81_05060"
     CDS             complement(3747..4202)
                     /locus_tag="PLY81_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNF29628.1"
                     /translation="MKFTAYLFFVMAFFSSCTTLDVYERTTFFNNHQWKSQQPVQAVF
                     EISDTTTLYNLYFVLRHEDAYKYNNIWVDISMQSPDTTISVKRNFVLGDNEKWLGSSM
                     SDIIEHRIKFSTAPAKLKKGKYIFTLHQIMREDPLHSILNAGIRVERTK"
     gene            complement(4251..5126)
                     /locus_tag="PLY81_05065"
     CDS             complement(4251..5126)
                     /locus_tag="PLY81_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNF29629.1"
                     /translation="MLYSMTGYGRGEKIIGDKTFLIEIKSLNGKQFDLRLFLPNLLKP
                     FEFEIRSKIAEDLERGSVECVITIKQNGINKPVSINTELLKAYYKSISEIAEELNADK
                     TQLMAAILRLPEVVSNSTEILSDTEWKEVEALLKNVLSDINEHRKEEGKSSECDLLQR
                     LVSIEKLQDEVKTLEPLRREKIKEGLQKLLIEYVGVNNYDTNRLEQELIYYIEKIDIS
                     EEQVRLINHCNYFRNILEEPTKAKGKKLSFILQEFGREINTTGSKANNADIQKKVVLM
                     KDELEKMKEQVLNVL"
BASE COUNT         1773 a          869 c          743 g         2068 t
ORIGIN      
        1 aatataaaac taaaacaaac agccttagaa agctattaca acttaaccca aaataaagaa
       61 gtattaaagg cattttcaac aagtaattta gaagagatga aagcaactgc agaaatactt
      121 aacaataagg aaaacccaaa tccctacttt tctaaagaag agtttttaaa caaattacaa
      181 ttaaactcta ttgttaatca atatcaatta cagtatttat gccgctttgc aataggttta
      241 gaaaacgaaa ataaattaaa taataatttc attgctgtaa ataaaacaac agctttagaa
      301 tatattttaa aagtgaaagc caataataat caaaaaattt tattagatat aattgggcta
      361 agtgttttat ttaggttgca acaagaataa aaatagactg gttgtttact ataatatatt
      421 tagcagagaa cccatataac ataaaaaact tttttctttt caatcatcgt ttatctttac
      481 accatacatt gctttatgat agaaactatt ttacttcctt ccgatttttc tgcaacagcc
      541 gttaatgcag gaatttacgc tattgagtta gcccagcaag ttggagctaa aaaaattgtt
      601 gtgtaccaca cttatgacgc tgccagcgtt tctgacccaa tggttagcta cactcaaaaa
      661 attgtaacag agcctttcag acaaaaaagt gctgcacaat taaatgagtt tatagataaa
      721 cttaatgcta aaaacaaaag cagtattgta attgaagcat ttcatagcta tgcagattta
      781 aaagatgcat tgaatgagtt aattgcctct accggtgcac aattaattgt aatgggcatt
      841 actggtggtg gagtatttaa agaaactttt attggtagta actctgtaaa cgttgcaaaa
      901 agtaccaata ttcctgtaat aatagtacct accgaaacaa gctttagccc aattagtaat
      961 attttattac ttagcgattt aaataatgta gcagatacta caccaacaga agctattaaa
     1021 aatattttag atgcctctaa agcaaaattg catgttttaa atgttacgga taatacggaa
     1081 aaagcagaag cttcttctga taaacaacag ttaggcaaat tatttgaagc ttataaccct
     1141 acttttaatt ttatgagtaa cccagatttt gtgggtgctg tagattggtt tgttaaagaa
     1201 aaaggaattg acttggtgat tgtaatacct aaaaaacatg gtttatttga aacattattt
     1261 aaatttaacc acactaaaac acttgccttt catggaaaag tgccattaat ggctgtaaaa
     1321 aaataatctc ataaaaaaac ctctttttaa agaggttttt ttgtatagga gtgttcaact
     1381 acttttttgc tggtggcatt aatacatcat taattacatg tatcatacca ttagatgttg
     1441 gtatagaagc tactatttca gaaccattta caaaggtctt tccatctttt ttggtaatag
     1501 taacattacc ctcaaacacc atatttaatt cttggccatc ttgcatcatt tctgctttaa
     1561 tagagcctac ataagtatgg tagcctagag cgttttctaa tttgccttta ttttctggtt
     1621 ttaataaatc ttccactgtg cctgcaggta atttatcaaa tgctgcattg gttggtgcaa
     1681 aaactgtaaa agggccagca ttgcttaaag catctaataa gccagctgct ttaactgctg
     1741 caactaaggt tgtatgatct ttactgcctg ctgctgtttg tacaatattt tttgctgatt
     1801 gatcgtcaac aacaccagat tggccaactg catctgtggt ttgggtagta gattctgaac
     1861 ctgttgtagt ttgctcacct gaacaagcag ttaataaata cattgccatt gtggtaagca
     1921 caataaacgt ttttttcata acaaaaatgg tttagggttg attgaaaatg caaagttgat
     1981 atgagaaaaa acaataaagc atgactgtaa tcacacagaa ggaaaatatt ttgaatgttt
     2041 taaagctggt ggtttaagta gttaatcagt aaacttataa ccaatacccc taacactatg
     2101 aaagtgttta gattgcttac tattttcttc gaaatgttta cgaaacatta atataaaatt
     2161 atctaccgtt cttgtagtag gaaaaacatt atagccccaa acaaccaata atattttttc
     2221 tctaggtaca acttcatttt tattttctat taataacttt aaaagattag tttctttttt
     2281 actaagctta attttttttc catcataggt tattgcatct aacgatttaa agtcaattaa
     2341 gttatttcca aaagaataag tatcgccaag tgtgctttct ttatcgtaaa tttttttatt
     2401 tttttgaatt agcttttcta cccttaataa taactcttct aaattaaatg gcttggttaa
     2461 ataatcatct gccccttttt ttaaacccaa cactctatct gcccctgaat ttttagcact
     2521 tagcattaat atgggcaact catcattact taatcttact gtttgtgcaa cagttattcc
     2581 atcaatttct ggtagcataa tatctaaaat cattaaatca aaatgggctt tgcttaattc
     2641 agttaaagcc tcgtctcctg cataagcact tgtaacatca tacccttcca tttctaagtt
     2701 taacttcagg gtttcgttaa ggccttcttc atcttctacc aataatatgc atgctttatt
     2761 ctctatcata atttacatat taaaaacggc tgtaaaaata cttccttgtg gatgattgtt
     2821 tgtcatggta atgtttccat tgtggctttt cataattttt ttacataagt ataagcctaa
     2881 gcctgttcct ttagttgttc ttgtcgattc gctaccagat ctataaaatt tatcgaatat
     2941 tttttttcta tcttgtggtt ttacgccaca acctttatct aatacatcta aaaatgtttg
     3001 cttgttgctt gtatataatc taatagttac gggcatttct ttgggtgaat attttaatgc
     3061 gttttcaatt aaattattta caagcatttg caacaacaat gcttcgccat ttataaataa
     3121 ttgctgttgt atatctgctt ctaattttcg ttgagggtat cttatggcaa agtcttctat
     3181 gcaggtttct gttaggtcgc ttaaatttaa ttcgtatgta ttactgtaat atgctcctcc
     3241 atctaattga gcagcaacta aaatattatt acataaactg tttaatctat ttgcttcctg
     3301 aattgtgtta gaaataagtt tctgttgttt ttcttcgtct aattttcttt tttgtagagt
     3361 ttctaaattt aaatgagtaa tggcaatagg tgttttaagt tcgtgtgtaa ccgccatcat
     3421 aaaattttgc tgctgaaccg atagtcttat ttgctttctg gttgccctat acacaaatac
     3481 tgccccaatt agtattactg ctaaaaaagt agttccttct cccaggtatt gtgcaatttt
     3541 ccttttttta gttgcttcta ttgtagaaat tttttcttta tataaagaat cagatgtttg
     3601 caataaagca atttgcattt ctgcagtatc gcttgcatga tggttaagtt taataaacca
     3661 aaataacaat gcagcaatca tgtacagtaa taaaaaccaa tatactgtgg taattaaggc
     3721 taatttttta tggaaaaaag atttcattat ttagttcttt ctactcttat ccctgcattt
     3781 aaaatactat gcaatgggtc ttctctcatt atttgatgaa gagtaaaaat atattttcct
     3841 ttcttaagtt tagcgggtgc cgtgctaaat tttattctgt gttctattat atcactcata
     3901 gaggagccaa gccatttttc attatctcct aatacaaaat ttctttttac agaaatagtg
     3961 gtatctggtg attgcatgga tatatccacc caaatattat tatacttata tgcatcttca
     4021 tgccttagca caaaatataa gttatataaa gtagtagtat cggaaatttc aaaaaccgct
     4081 tgaactggtt gctgactttt ccattgatga ttattaaaaa aagttgttct ttcatataca
     4141 tctaacgttg tacacgaaga aaaaaatgcc atcacaaaaa ataaatacgc agtaaatttc
     4201 attttaaaac ttgttgaaat aataaatttc tatctaaaca attttgggtt ttataataca
     4261 tttaatacct gttccttcat tttctctagt tcatctttca ttaatactac tttcttttgt
     4321 atatcagcat tatttgcttt gctgcctgta gtattaattt ctctgccaaa ttcttgtaaa
     4381 ataaatgata attttttgcc cttggctttg gttggttctt ctaaaatatt tctaaaataa
     4441 ttacaatgat ttattaatct tacttgttct tcgctaatat caattttttc aatataataa
     4501 atcaattctt gttctaatct gttagtatca taattattta ctccaacata ttctataagt
     4561 agtttttgta atccttcttt aattttttct cttcttaaag gttctaacgt tttaacttca
     4621 tcttgcaatt tttcaatact aacaaggcgt tgcagtaaat cacattcaga actttttcct
     4681 tcttcttttc tatgctcatt aatgtcagat aaaacatttt ttaataaggc ttccacttct
     4741 ttccattcag tatcacttaa aatttctgtt gagtttgaaa ccacttctgg taatcttaaa
     4801 atagctgcca tcaattgtgt tttatccgca ttcaattctt ctgctatttc tgaaatgctt
     4861 ttatagtatg cctttaagag ttcagtatta atacttacag gtttattaat tccattttgt
     4921 ttaattgtaa taacacattc aacacttcct cgttctaagt cttcggcaat tttgcttctt
     4981 atttcaaact caaagggttt taataagttg ggtagaaata accttaaatc aaattgtttt
     5041 ccgtttaatg attttatttc aattaaaaat gttttatcgc caattatctt ttctcctctt
     5101 ccataacctg tcattgaata aagcataata aagtattact gtttaatgta aagcaatttt
     5161 aaaagtatct cgtaaaagta gtttaaaaaa tttaatgcaa acatacaatt catctattgt
     5221 actttttgag gttaattaat taaataaata aaaattacgt gtttagtatt attaatgcaa
     5281 tctatcttgt taattttaac cttctaaact agattatgta tagcaataaa gagcaatggc
     5341 tacaacagtc aaagttattt ttaagtaata gtttaaatag ccagtttgtt ttatctaata
     5401 ttaacactct tagggctgtt ttgcagtttc atgaatataa ttattatgtt tta
//
LOCUS       DAOQHP010000118         5450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8426_length_5450_cov_3.341983, whole genome shotgun sequence.
ACCESSION   DAOQHP010000118 DAOQHP010000000
VERSION     DAOQHP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_5450_cov_3.341983"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLY81_05070"
     CDS             complement(<1..248)
                     /locus_tag="PLY81_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNF29630.1"
                     /translation="MTTEDFLKIVSTIPTLPGIYKYFDIENELIYVGKAKNLKNRVRS
                     YFGKSVNNYKTHKLVNAITRIEFTIVDSEQDAFLLENA"
     gene            complement(264..839)
                     /locus_tag="PLY81_05075"
     CDS             complement(264..839)
                     /locus_tag="PLY81_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29631.1"
                     /translation="MKYILTFSTFFILLLSCKEQKKEQEVSQNNIDTLPYYNYTAAIN
                     TEADSLIKIKKQFLKIVTNPNNSNNASKISLSDFKNLVQLFTQQNISEPPLKQFYKEQ
                     IFTDLTTNSTIINYTTSKDSLPVKNISIMLDAKNANTLKRIDIKNIYSVPDTLITENY
                     AWIFGKEFYFVKYYEPTNSIAFTTKTNISFK"
     gene            918..1997
                     /locus_tag="PLY81_05080"
     CDS             918..1997
                     /locus_tag="PLY81_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29632.1"
                     /translation="MKKSFYWILFAICFIHNYVFANMASPYIGGTKQSSAISSKHIQI
                     HSENIHIAIDKNFKTAKFIVEYNILADTNGLQVPLLFIAEDFKENFKVWVDGNLVEIK
                     NVNELLTNNPTAFNQLFNGINNTNEISIYWDKNIGNVYQLRDLKYFETDIAKGNHKIK
                     VEYVASVQIDRSNWVKNYKLKYSLSPAKFWKSFGSLAITLELEGTQKVTTNLGLPIEK
                     NYSTINNWKFTTLPADFIEISFTPTISSYAETLISMGPFGISMVFTILLFAINLYLIF
                     WYRRSNINKKYSPPVIIGSLLIPLISIIIYMYSYSFIDNAIGEEAGKYHGYFILVIII
                     YPILFLVYLLFTWLIDKLYKRKLLK"
     gene            complement(1989..3419)
                     /locus_tag="PLY81_05085"
     CDS             complement(1989..3419)
                     /locus_tag="PLY81_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF29633.1"
                     /translation="MYQHNNDKDNLEQFLQTVADKNKMYSDEKVWNAIYKELHGYKRW
                     NALTIISFIVITVLTVFTILFNNTVKLNFIASNTFKEVKNKVPKKATTALISENIIPQ
                     QKIFSQSNKSYNTYPTLAFIEKESLPEAVHSKTNKSISLAFLDKLFLNNYPNLNGYIA
                     TTNSIEEPTINSIYNLKHSTLNINANTSLLAALNTVPKQEEQIILPKVIIQENPTTEI
                     EKYISKTAFTKNKKSAIELQVYATPSMSYRKLVDDKTRNTISLRDGNTTTPLPSVYYV
                     DVNEVVHHKPALGLEAGVGILYKLTDKVKLRTGLQFNIRKYYIDSYKSGLNIAQIAVI
                     TNNGLDTINQFATSSTTGPLNKSLKNSLYQISIPLGIQWSFLQSKNFEIGLGLSIQPT
                     LTLNKNVYLISTDYKYYTDGTSFFRKWNINTGLDLNFIYKVGNYSFFLSPQLRYQHLP
                     TYTDEYPIKEYRLDNGLRFGFTKKLF"
     gene            complement(3433..4008)
                     /locus_tag="PLY81_05090"
     CDS             complement(3433..4008)
                     /locus_tag="PLY81_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF29634.1"
                     /translation="MIDQSLIQGCIDNDATAQRALYNTFSPKMMSICYRFAYNKNDAQ
                     DILQEAFLKIFSQIHTYQFKGSFEGWVRRIVVNSCINYIKRNKKFNDNVDIANAYNIQ
                     IKENNIGQMMFTKQVVECIRLLPIGFRTVLNLYAIEGYSHKEISEILDIQESTSRSQY
                     TRAKVILENILIKKGLIKNKEEELNWIAELK"
     gene            complement(4115..>5450)
                     /locus_tag="PLY81_05095"
     CDS             complement(4115..>5450)
                     /locus_tag="PLY81_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNF29635.1"
                     /translation="FGLNIDRASYSNIRRFLNNGVTVPVNAVRIEEMLNYFDYNLNRD
                     KNITKVNEFCVKNYLSTCPWDNNSSLLFLTINAPKLNLDTIPPCNLVFLIDVSGSMDK
                     PNRLPLLQSAFKMLVDNLRDIDLVTIVIYGGGVAKVLDATSGSEKVAIKNVIDSLSAS
                     GDTPGQAAIQTAYASVAKTFIPGGNNRVILATDGDFNVGVNSEKALEDLITGYMQSGI
                     YLTCLGVGMGNYKDSKLETLAKKGRGNFAYIDNLAEAQKVLVSEFTKTLYSVANDASI
                     NIEFNSKNISEYRLIGFDNKKSAIIDNNSELEGGEVGSGHNMIAIFELKDLPNSKEKI
                     NIGEATLQFKTTDSSKKLIIQNFNLANNIQAFDTISNTLKFATAVSMFGSLLKQSKYA
                     NGYDFETIYRIALPTVLPNEYAKQEFLRLLFTAEKIYNPKRKRHNKKQEGFR"
BASE COUNT         1821 a          736 c          751 g         2142 t
ORIGIN      
        1 aatgcatttt ctagcaaaaa ggcatcttgc tcactatcta caattgtaaa ctcaatacgt
       61 gttatagcgt ttacaagttt atgggtttta taattattta ccgatttgcc aaaataactt
      121 ctaactctat tttttaaatt tttggcttta ccaacataaa taagttcatt ttctatatca
      181 aagtatttat aaattccagg aagggtagga attgtagata caatttttag aaaatcttca
      241 gtagtcataa tagcttttaa taattattta aagctaatgt tagttttggt tgtaaaagca
      301 atagaattgg taggctcgta gtattttaca aaataaaatt ctttgccaaa aatccatgca
      361 tagttttcag taattaaagt gtcgggtact gaataaatat ttttaatgtc aattcttttt
      421 agtgtgttgg catttttagc atctaacatt atacttatat ttttaacagg taaactgtct
      481 tttgaagttg tataatttat aatggtacta ttagtagtta aatctgtaaa aatttgctct
      541 ttataaaact gttttaacgg aggttctgaa atattttgtt gggtaaataa ttgaactaga
      601 tttttaaaat ctgaaaggga aattttagat gcattattag aattattagg attagttact
      661 atttttaaaa attgtttttt aatttttatc aaactatccg cttctgtatt aatagctgct
      721 gtatagttat aataaggcaa tgtatctata ttattttgag atacttcttg ctcttttttc
      781 tgttctttac aacttaataa taaaataaaa aatgtactaa aggttaaaat atatttcata
      841 aataaaatta gttgatattg ttttaatggc ttatttttcc tgtaaagaaa tgattaattg
      901 ataaaaatag tttaaaaatg aagaaatctt tttattggat attatttgca atctgtttta
      961 ttcacaatta tgtttttgcc aatatggctt cgccatatat aggaggcaca aagcaaagtt
     1021 ctgctatttc tagtaaacac attcaaattc attcagaaaa cattcatatt gctattgata
     1081 aaaattttaa aacagcaaaa ttcattgttg agtataatat tttagcagat accaatgggc
     1141 tacaagttcc attattgttt attgcagaag attttaagga aaattttaaa gtatgggttg
     1201 atggcaattt ggttgaaatt aaaaatgtta atgaacttct tactaataac cctacagcat
     1261 tcaatcaatt gtttaatgga attaataaca ccaatgaaat aagtatttat tgggataaaa
     1321 atattggcaa tgtttatcaa ctaagagatt taaaatattt tgaaacagat attgccaaag
     1381 gtaatcacaa aataaaggtt gaatacgttg catcagtaca aatagataga tctaattggg
     1441 taaagaatta taaacttaag tattcattgt ctcctgcaaa gttttggaaa tcatttggca
     1501 gtttagcaat tactttagag ttagaaggaa cgcaaaaagt tactaccaat ttaggattac
     1561 ctatagaaaa aaactattct acaattaaca actggaagtt tactactttg cctgccgact
     1621 ttattgaaat atcttttaca ccaactatta gcagctatgc tgaaacatta ataagcatgg
     1681 gcccgtttgg tataagtatg gtttttacta tactattatt tgctattaat ttgtatttaa
     1741 ttttttggta tagaagaagt aatattaata aaaaatactc tcctcctgta attataggaa
     1801 gtttattaat acccttaatt tctataatta tttatatgta tagttattct tttattgata
     1861 atgcaatagg tgaggaagca gggaaatatc atggatattt catattagta attattattt
     1921 atcctatttt atttttagtt tatttattat ttacctggct aattgacaaa ctttataaaa
     1981 gaaaattatt aaaataattt tttagtaaat ccaaatctta aaccattgtc tagcctgtat
     2041 tcttttattg gatattcatc ggtatatgta ggtaaatgtt gatagcgtaa ttgtgggctt
     2101 aaaaagaaac tataattacc aactttgtaa ataaagttta aatctaatcc agtattaata
     2161 ttccattttc taaaaaatga agtaccatct gtataatatt tataatcggt tgaaattaaa
     2221 taaacatttt tatttaaagt taaagttggc tgaatactaa ggcctaaacc aatttcgaaa
     2281 tttttagatt gtaaaaaact ccattgaata ccaagtggaa ttgaaatctg gtataaacta
     2341 ttttttaaag atttatttaa aggccctgtg gtactagaag tggcaaattg attgatggta
     2401 tctaaaccat tattggtaat tactgcaatt tgagcaatgt ttaatccact tttataacta
     2461 tcaatgtaat atttacgaat attaaattgt agtccagttc ttaacttaac tttatcagtt
     2521 aatttatata aaatacccac accagcttct aaacctaatg caggtttatg gtgtaccact
     2581 tcattaacat ctacataata aacagaaggt aggggagttg ttgtattacc atctcttaaa
     2641 ctaatggtat ttctagtttt gtcatcaact agttttctgt aactcatgct aggggttgca
     2701 taaacttgta actctatagc agatttttta ttttttgtga aagctgtttt agaaatgtat
     2761 ttttctattt ccgttgttgg attttcttga ataataactt taggtaatat tatttgttcc
     2821 tcttgtttag gtactgtgtt taaagctgct aataagctag tgtttgcatt aatatttaag
     2881 gtagaatgtt ttaaattata aatactatta atggtaggct cttctattga gtttgtagtg
     2941 gcgatatagc catttaaatt ggggtaatta ttcaaaaaca acttatctaa aaaagctaag
     3001 ctaatactct tgtttgtttt tgaatgaacg gcttctggta gcgattcttt ttcaataaat
     3061 gctaaagttg gatatgtatt gtatgattta ttagattggc taaaaatttt ttgctgcgga
     3121 ataatatttt ctgaaattaa tgctgtagtt gcttttttgg ggactttatt ttttacttct
     3181 ttgaaagtgt tactggctat aaaattaagt ttaaccgtat tattaaataa aatggtaaaa
     3241 actgttaaaa ctgtaataac aataaaagaa ataattgtaa gggcattcca ccttttataa
     3301 ccatgtagtt ctttgtaaat tgcattccaa actttttcat cagaatacat tttattttta
     3361 tcagcaactg tttgtaaaaa ttgctctaag ttatctttat cgttattatg ttggtacact
     3421 ttaaacttac atttatttta gttctgcaat ccagtttagt tcttcttctt tgtttttgat
     3481 taaccctttt ttaattaaaa tattttctaa aattactttt gctctggtat attggcttct
     3541 gctagtactt tcttgaatat ctaaaatttc tgaaatttcc ttgtggctat acccttcaat
     3601 ggcgtataaa tttaatactg ttctaaaacc aattggcaat aatcttatac attcaactac
     3661 ttgttttgta aacatcattt gcccaatatt gttttcttta atttgtatat tataagcatt
     3721 ggctatatcc acattatcat taaatttttt atttcttttt atataattaa tgcaactgtt
     3781 tacaacaatt cttcttaccc atccttcaaa agagccttta aattgatagg tgtgaatttg
     3841 agaaaagatt tttaaaaaag cttcttgtaa aatatcttga gcatcgtttt tattataggc
     3901 aaaacggtaa caaatactca tcatttttgg gctgaatgta ttgtacaatg ctctttgtgc
     3961 agtagcatca ttatctatac aaccttgtat taaactttgg tcaatcattt tgagtactga
     4021 ttatttaacc ctaaatgtat gacagttttt ttagaaatct gctgctttta tacttaattg
     4081 tataggcggt atttaaaagt aaaggaaaat atatttatct aaacccttct tgctttttgt
     4141 tatgcctttt ccttttggga ttataaattt tttcggcagt aaataatagc cttaaaaact
     4201 cttgtttagc atattcattg ggtaatacag ttggtagagc aatacgataa atagtttcaa
     4261 aatcgtaccc atttgcatat tttgattgtt ttaataaact accaaacata gaaacagcag
     4321 tagcaaattt taaagtatta ctaattgtat caaaagcttg tatgttgtta gctaagttaa
     4381 aattttgaat gattaatttt ttgcttgagt ccgtagtttt aaattgtagt gtagcttccc
     4441 caatatttat tttttctttt gaattgggta ggtcttttaa ttcaaaaatg gcaatcatat
     4501 tatgaccact tccaacctca ccaccttcta gttcactatt attatctatt attgccgatt
     4561 ttttattgtc aaacccaatt aatctatact cagaaatatt ctttgaatta aattcaatat
     4621 taattgaagc atcattggct acagagtata aagtttttgt aaattcagat accagaactt
     4681 tttgagcttc tgcaagatta tcaatgtatg caaaattccc tcttcctttt ttagctaaag
     4741 tttctaattt actatcttta taattaccca taccaacacc caaacaagtt aagtaaatgc
     4801 cactttgcat ataaccagtt attaggtctt ctaaagcttt ttcggagttt acgccaacat
     4861 taaaatcgcc atctgttgct aatattactc tgttgttgcc acctggtata aaagtttttg
     4921 caacagatgc ataagctgtt tgtatagcag cttggcctgg agtgtctcca ctggcagata
     4981 aagaatcaat tacattttta atagctactt tttcgctacc acttgttgca tctagcactt
     5041 ttgctacacc acctccataa attacaattg ttactaaatc tatatctctt aaattatcta
     5101 ccaacatttt aaatgccgat tgcaaaagtg gtaacctatt aggtttatcc atagaacctg
     5161 aaacatctat caaaaaaact aaattacaag gaggtatagt atctaaattt aattttgggg
     5221 cattaattgt taaaaataat aagctgctgt tattatccca agggcaggtt gataaataat
     5281 tttttacaca aaattcatta accttagtaa tgttcttatc tcggtttaaa ttataatcaa
     5341 aatagttgag catttcttct attctaacag catttactgg tactgtaact ccattattta
     5401 aaaaccttct gatgttacta taagaagcac ggtctatatt taaaccaaaa
//
LOCUS       DAOQHP010000119         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8459_length_5437_cov_3.736343, whole genome shotgun sequence.
ACCESSION   DAOQHP010000119 DAOQHP010000000
VERSION     DAOQHP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8459_length_5437_cov_3.736343"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PLY81_05100"
     CDS             <1..900
                     /locus_tag="PLY81_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNF29636.1"
                     /translation="GIFPVITDDFEELRDCMEKLQLNDASLTFELETSQALGFGFRCG
                     FLGLLHMEIIQERLEREYNQTVITTVPNVSFFAYTTRGEKIVVNNPAEMPETTKIEKI
                     EEPFIKAQIITHSDYIGNIMTLCLGKRGILINQSYITTTRVELIFEMPLTEIVFDFYD
                     KLKSQTRGYASFDYSPIGYRDADIVRMDILLNAEKVDALSALIHRSRSQDFGRKLCEK
                     LKELLPRQQFQIAIQAAIGAKIIARENISAIRKDVTAKCYGGDISRKRKLLEKQKEGK
                     KRMRQIGTVEVPQEAFLAVLKLD"
     gene            complement(906..2837)
                     /locus_tag="PLY81_05105"
     CDS             complement(906..2837)
                     /locus_tag="PLY81_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF29637.1"
                     /translation="MQKSSTLLLQFFYLILLVVCSYTLCRIFFVLFNLKELDIPTFNS
                     FATILWHGLRFDVAAIAVTNSLFIILFFLPVKLATNKLYQLVLKVIFIIINSFCIIAN
                     LMDVAYFPFVHKRSQADALLFFNGQKGNDFFRLIPTFLAQYWYLLFIVGLLIYLLYFF
                     YKKIIINSNLKNTLTIKHYSLRAIVFLMAIGITIIAIRGGFQTKPLDIIHASEVVSAK
                     NIAAVINTPFSIIKTVDKKSLEEKIYFPTSVLSELNNGIHLPPSNHSFTKKNVVIIIL
                     ESVSKRHIHYFGSKAATPFLDSLFNQSLVFTNAFANAKESIQGIPAILSSIPSLQTDP
                     FIFSSYASNKITSLPEVLKAEGYTTSFFHGGFNGTMGFNSYAKLAAFDNYYGMNEYNN
                     SKDYDGKWGIWDEPFLQFTSQKISETKEPFMASIFTLNTHHPFQIPQKYSHKFNKHQE
                     PFLNCIEYLDFALQQFFNISKNKPWFNNTLFVITADHTAPLLKNETTTLMDDFRIPLV
                     FFQQNNHDLKGINNSIANQIDILPSVLANLNYNKPYFSFGNNLFSNSTIKNAIQFTGN
                     IYQYIDSTNFYQFNGEQGIAFYNWPNDSLLKNNLINKANSFALLKSDSCLKKKIQFYN
                     SAMINNKMNISSIRINQKN"
     gene            complement(2883..3560)
                     /locus_tag="PLY81_05110"
     CDS             complement(2883..3560)
                     /locus_tag="PLY81_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29638.1"
                     /translation="MKKNVLFITLFLFFGVANAQKLVKLIQKNDAYFVEYDNKVQKEI
                     IQGKGNSVIYYSKKNQYVLYKKLIKKSVLMNKEDGREDAADMYSIYIFNILNNKTIEV
                     FKSCLDGNDGTKPSYANSKIYPFNSICNPDNFMLSPDENIIFFNSKAWAVSNAIHYFS
                     INDLMLKFFHSGSLIKVDNNGVTIEISDVETVRENGEIVSKGRYWQKCLYDFNGKFIK
                     YIGEKNR"
     gene            complement(3669..3739)
                     /locus_tag="PLY81_05115"
     tRNA            complement(3669..3739)
                     /locus_tag="PLY81_05115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3705..3707),aa:Cys,seq:gca)
     gene            complement(3829..5157)
                     /gene="gldE"
                     /locus_tag="PLY81_05120"
     CDS             complement(3829..5157)
                     /gene="gldE"
                     /locus_tag="PLY81_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNF29639.1"
                     /translation="MNDFSIFFKPTLVTIIDSQGTTILFVLLLFLLVLSFILSGAEVA
                     FFSLNYKDISVLKTKQQPVYKRIVTLLENPKTLLGSLLIANTFSNISIIIISNVLLDN
                     LIHFHPNFEWVEFLIKVTLVTFMLVLFSEVMPKVMATQNNVRFAKDVGWLVEIVSYVF
                     NSLSNWLVKYSDLVEKKLSNKNLNNNTNEELYHAIDITHTDSNESEKNILKGILKFGN
                     TTVKQVMKTRLDVHGIDYNISFSELLQLINELHYSRLPVFKDDLDEIAGIIHTKDILQ
                     YLDADNNFNWHSLLRQPYFVHEQKMIEDLLSEFQSKRIHFAIVVDEFGGTSGIVTMED
                     IMEEVIGEIADEFDDEDAGQKKIDDRNYIFEGKTMIRDVCKFMNLSIDTFDGIKGESD
                     SLAGLILEIAGEIPYVTQVITCGDFEFTMLEVAKNRINKIKVTIVPHMVE"
     gene            complement(5235..>5437)
                     /locus_tag="PLY81_05125"
     CDS             complement(5235..>5437)
                     /locus_tag="PLY81_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF29640.1"
                     /translation="IYLEGRLRTRSWEDKEGNKKFATEIVGDNLIMLDKRNEGSHSAN
                     DIIDDGYNDNQSLPNTDENLPF"
BASE COUNT         1975 a          775 c          775 g         1912 t
ORIGIN      
        1 ggaatttttc cagtaattac cgatgatttt gaagagttga gagattgtat ggagaagctg
       61 caattaaatg atgcttcctt aacctttgaa ttagaaacat ctcaggcact tggttttgga
      121 tttagatgtg ggtttttagg tttattacac atggaaatta ttcaagagcg tttagaaaga
      181 gaatataacc aaactgtaat aactactgtt cctaacgtta gcttttttgc atatacaaca
      241 agaggcgaaa agatagtggt gaacaaccca gcagaaatgc ctgaaacaac aaaaattgaa
      301 aaaatagaag aaccttttat taaggcacaa ataattaccc attcagatta tattggtaat
      361 attatgacac tttgcttagg taaacgtggc atactaatta atcaatctta cattactaca
      421 acaagggttg aactaatatt tgaaatgccc ttaacggaaa tagtatttga tttttatgat
      481 aagttaaaaa gccaaacaag gggttatgcc tcatttgatt attctccaat tggctataga
      541 gatgcagata ttgttagaat ggatatttta ttaaatgcag aaaaagtaga tgctttaagt
      601 gctttaattc atcgtagtcg ttctcaagat tttggaagaa aattatgtga aaaactgaaa
      661 gaacttttac ctcgccaaca atttcaaatt gctatacagg ccgcaattgg tgcaaaaata
      721 attgcacgtg aaaatattag tgccattaga aaagatgtaa ctgcaaaatg ttatggtggc
      781 gatataagca gaaaaagaaa attattagaa aaacagaaag aaggcaaaaa acgaatgaga
      841 caaattggaa ctgtagaagt accacaagaa gcctttttag cagtgttaaa gttagattaa
      901 actccttagt ttttctgatt gattctaatg ctagaaatat tcattttgtt attaatcatt
      961 gcactattat aaaactgaat tttctttttt aagcaactat cacttttaag taaagcaaag
     1021 gaattagcct tattaattaa attgtttttt agtaaactat catttggcca attataaaaa
     1081 gcaatgcctt gttctccatt aaactggtaa aaattagttg aatctatata ctgataaatg
     1141 ttacctgtaa attgaattgc attttttatg gtagaattac taaataaatt atttccaaag
     1201 gaaaaatagg gtttattata gtttaaatta gctaaaacgc tgggtaaaat atctatttgg
     1261 ttggcaatgc tattatttat gccttttaaa tcatggttat tttgctgaaa aaataccaat
     1321 ggaattctaa aatcatccat cagtgttgtt gtttcatttt ttagcaatgg tgcagtatgg
     1381 tcggctgtta taacaaacaa agtattatta aaccaaggtt tgttttttga aatattaaaa
     1441 aactgttgta atgcaaaatc taaatattca atacaattta aaaacggctc ctggtgctta
     1501 ttaaatttat gtgaatactt ttgaggtatt tgaaaaggat ggtgtgtatt taaagtaaaa
     1561 atggaagcca taaaaggctc tttagtttca gatatttttt gggaagtaaa ctgcaaaaaa
     1621 ggttcgtccc atattcccca ttttccatca taatcttttg aattattata ttcattcata
     1681 ccataataat tatcaaaagc agcaagttta gcatagctgt taaagcccat tgtaccatta
     1741 aaacctccat gaaaaaagct ggttgtatat ccttctgctt tcaacacttc tggtagagat
     1801 gtaattttgt tggatgcata agaagaaaaa ataaaaggat ctgtttgcaa agaaggaata
     1861 gaagaaagta ttgcaggaat gccttgaatg gattcttttg cattggcaaa ggcattagta
     1921 aaaacaaggc tttggttaaa taaggagtct aaaaatggag tagcagcttt acttccaaaa
     1981 taatgaatgt gtcttttaga aacactttct aaaataataa taactacatt tttttttgta
     2041 aaagaatgat tggaaggtgg aagatgaatg ccattattca attctgataa tacagaggtg
     2101 gggaaatata ttttttcctc taaacttttt ttatctactg ttttgatgat ggaaaatggt
     2161 gtatttatta ctgcagcaat attttttgca gaaactactt ctgaggcatg aataatatct
     2221 aaaggttttg tttgaaaacc tcctctaatt gcaattattg taattccaat agccattaaa
     2281 aatacaattg ctcttaaact gtaatgttta atagttaaag tattttttag gttactattg
     2341 atgataattt tcttgtaaaa aaaatataac aaataaatta gtaatccaac tataaatagt
     2401 aaataccaat actgagctaa aaaagtaggg ataagcctaa aaaaatcatt acctttttga
     2461 ccattaaaaa ataataatgc atctgcctga gaacgtttgt gtacaaacgg aaaatatgca
     2521 acatccatta aattggctat aatgcaaaag ctatttatta taataaaaat aacttttaac
     2581 actaattgat atagtttatt ggttgctaat tttactggta aaaaaaataa aattataaat
     2641 aaactattag taactgcaat ggctgcaaca tcaaatctta aaccatgcca tagtatagtt
     2701 gcaaatgaat taaaggttgg aatgtctaat tcttttaaat taaataaaac aaaaaatatt
     2761 ctacaaagcg tatagctaca aacgacaagt aaaataagat agaaaaactg aagaagtagg
     2821 gttgaagatt tttgcattct ctaataaatt cagtagcaaa attatagtta agcactactt
     2881 ttttacctgt ttttttcgcc aatatattta ataaatttac cgttaaaatc gtataaacat
     2941 ttttgccaat aacgtccttt gctaactatt tcaccatttt ctctaactgt ttcaacatca
     3001 gatatttcaa ttgttacacc attgttgtca accttaatta gtgaaccaga atgaaagaac
     3061 tttaacatta aatcgttaat actaaaatag tgaatagcat tagatacagc ccatgctttg
     3121 gagttaaaga aaataatatt ttcatctggg ctcaacataa aattatccgg attacaaatt
     3181 gagttaaatg gatagatttt agaatttgca taacttggtt ttgtaccatc atttccatcc
     3241 aaacaagatt taaatacttc aatggtttta ttgtttaata tattgaatat atagatagaa
     3301 tacatatctg cagcatcttc tcttccatct tctttattca ttaatacaga ttttttaatt
     3361 aattttttgt ataaaacgta ctgatttttt ttagagtaat atataacaga atttcctttt
     3421 ccttgtatta tttctttttg tactttatta tcatactcaa caaaataggc atcatttttt
     3481 tgaattaatt taactagctt ttgtgcattt gctaccccaa aaaataaaaa taatgtaata
     3541 aacaatacat tttttttcat acacattaat ttatacttta attctcaaat ataccaattg
     3601 tagttaagaa gaaagggggt tagagcaaat aaagttaagg taaataataa gaaaaaaagt
     3661 taggaaagag gtcccgacca gattcgaact ggtgtggaag cttttgcaga gctctgccta
     3721 gccactcggc cacaggacct tttaagatgg ttgcaaacat aaggtaaaac taaataaatg
     3781 caataagtaa ttttaggata atgtatgggt gatataaaaa atgttaagtt attctaccat
     3841 gtgtggtaca atggtaactt taattttatt tattctgttt ttagctactt ctaacatggt
     3901 aaattcaaaa tcgccacaag taattacctg tgtaacataa ggtatttcgc ctgcaatttc
     3961 taaaattaat cctgctaaag aatcactttc tccttttata ccatcaaaag tatcaataga
     4021 aagattcatg aatttgcaaa catctcttat catagtttta ccttcaaaaa tgtaatttct
     4081 gtcatctatt ttcttttgtc ctgcatcttc atcatcaaat tcatcggcaa tttctcctat
     4141 tacttcttcc ataatatctt ccatagtaac aataccactt gtacctccaa actcatccac
     4201 tactatagca aaatgaattc gtttgctctg aaattcactt aagagatctt caatcatttt
     4261 ttgttcatga acaaaataag gttgccttaa aagggagtgc cagttaaaat tattatcggc
     4321 atctaagtat tgtagtatat cttttgtatg aataattcca gctatttcat ctaaatcgtc
     4381 tttaaaaact ggtaacctgc tataatgtag ttcatttatt aattgaagta attcgctaaa
     4441 agaaatattg taatcaatac catgcacatc taatcttgtt ttcataacct gtttaacagt
     4501 agtattgcca aactttaaaa ttccttttaa aatatttttt tcactttcat tactgtcggt
     4561 atgtgtaata tcaattgcat ggtaaagttc ttcgttggta ttgttgttta agtttttatt
     4621 agataatttt ttttcaacta aatcgctata ctttactaac caattgctta aactattaaa
     4681 aacatacgat acaatttcta ccagccatcc cacatctttt gcaaaacgca cattattttg
     4741 tgttgccatt actttaggca taacctcact aaataaaaca agcataaagg ttactaaggt
     4801 tacttttatt aaaaactcaa cccattcaaa atttggatga aaatgtatta agttatctaa
     4861 taaaacattg cttataataa taatagaaat atttgaaaaa gtattagcaa ttaaaagaga
     4921 acctaataat gttttaggat tttccaaaag ggttactatt cttttataaa cgggctgctg
     4981 ctttgttttt aaaacactaa tatctttata gtttaacgaa aaaaaagcca cttcggcacc
     5041 acttaaaata aacgataata ccaataaaaa aagtaacaaa acaaacaaga tggttgtacc
     5101 ttgagagtca attatggtta caagagtagg tttaaaaaat atcgaaaaat cgttcaaaat
     5161 attttcttta gtgaataata tttatacaac tacttaggtt gtatgtgtta ttaattttac
     5221 aagaatacca acctttaaaa aggtaagttt tcatcagtat tgggtaaaga ttggttatcg
     5281 ttatagccat catcaattat atcgttagct gaatggctac cctcatttct tttatcaagc
     5341 attattaaat tatctccaac aatttctgtt gcaaattttt tgttaccttc tttatcttcc
     5401 caacttctgg ttctcaaacg tccttctaaa tatatta
//
LOCUS       DAOQHP010000120         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8500_length_5421_cov_4.369549, whole genome shotgun sequence.
ACCESSION   DAOQHP010000120 DAOQHP010000000
VERSION     DAOQHP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8500_length_5421_cov_4.369549"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PLY81_05130"
     CDS             <1..1405
                     /locus_tag="PLY81_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF29641.1"
                     /translation="EEQKQKYIPKLASGEFKGAYGLTEPNSGSDALSAKTTAKLSEDG
                     KYYILNGQKCWITNGGFADVYTVFAKVDGTQFTAFIIERGFEGFTQGPEEHKMGIKGS
                     STVQLYFQDCKVPVENLLGEIGKGHIIAFNILNIGRLKLCAAALGGSKSAATTSVQYA
                     NTREQFKLPIAKFGAIKHKLAEMAIKIWVTETALYRTAKWIDNKEHELLEAGKPFNEA
                     LLGAAEEYAIECAILKVFGSETLDFVVDEGVQIHGGNGFSDEYVISKAYRDSRINRIY
                     EGTNEINRLLTVDMVLKRAMKGKLDLMTPAMNVQKELMSIPDFGTEEDGAFSKELKYI
                     ANFKKAILMVAGAAVQKLMMQLDKEQEVLMNIADMAIEVFHSESALLRVIKLYNQHGA
                     EATSLQTDMMRTYLYDAADKINKAGKDALNSFADGDELRMMHIGLKRFTKVEPFNTKE
                     ARRRICNKMLEANAYCY"
     gene            1683..3671
                     /locus_tag="PLY81_05135"
     CDS             1683..3671
                     /locus_tag="PLY81_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF29642.1"
                     /translation="MFVISCNSNSKNKLNSERLPAIDTIIDKAGKISFLGGEPALKFI
                     DSATYNKKFSTKEKIRLFGYLSYLYSKYLHNIEKSALYSDSMFYLLNNKNIDDYSYEY
                     SYSYFTRGDVLFDQRKFGEAYSYYYKGKQVGVAFYDSATLGEYSYRLGHVLYLQGKFN
                     EAKLSFMQALELWKTATLTFGKYYATQEIISNIGLCYYKANQPDSSLFYFKKALNYID
                     NAEQNYINRKELHNAAKGVIFGNIGKVYEKTKQYDSALTYYKKGVIINLADGYEIEDA
                     LINNIHVAQVYGIQHKYDSMKNTLLQTEKWLKKSSEYKLANLMWHKQMWQYYDKTNQT
                     QQAYTYLTKYTELFTQHSKETISINSINIDQQVEFLESKKAIEVMQKESKIKEIYLYG
                     SILLVVLMSGFIVVTLIFWNKSKQNIKKLQQLNNTIQHQNFILETQSREKEKILKLVA
                     HDLRTPIASIAALTDMLEEEKEKEPQKELIGIMKMACDSSLKLISEILESSSSTNKEI
                     EKEMVSASDCLSDCISQLQINAAEKKQKFVIKPLPYAVSLYVNKDKMKRVMYNLITNA
                     IKFSHEGGEIHLKMEQHNDKSILISIQDFGIGIPDHIKTNIFNLNDEAKRSGTKGEKS
                     FGLGLTICKDIVAAHNGKIWVESTENKGTTFFVELPLS"
     gene            3770..4063
                     /locus_tag="PLY81_05140"
     CDS             3770..4063
                     /locus_tag="PLY81_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNF29643.1"
                     /translation="MRVIAKKVLRDFWEKHCDCEQQLKAWFLETSKAEWINPNEIKVE
                     YPSASIIGNDRIVFNIKGNKYRLIVKINFDYQMVWIRFIGTHSEYDKVNAKTI"
     gene            4065..4421
                     /locus_tag="PLY81_05145"
     CDS             4065..4421
                     /locus_tag="PLY81_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF29644.1"
                     /translation="MNIKPIKTKKDYEQAMLRLEKLFDAKKGTSKGDELEVISMLVEK
                     YEDEHFPIDLPDPVEAIKFRMEQLGMTQTDLANLVGQKSRASEILNRKRKLSLDMIRQ
                     LHQQWRIPTEVLVQAY"
     gene            complement(4594..5097)
                     /locus_tag="PLY81_05150"
     CDS             complement(4594..5097)
                     /locus_tag="PLY81_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF29645.1"
                     /translation="MDNIILRPWKKEDAQALAAIANNKNIWNNVLDNFPSPYTVTDAL
                     QWINRESAIIPVSKFAIECNHKIVGGIGLLTYDDIYKCSIELGYFIGEPFWGLGIATK
                     AIEKIVQHIKDDRPGVKRIFARVFENNKASMKALQKAGFYLEGIQKNAAVKNSELVNI
                     YVLVKLV"
     gene            complement(5183..>5421)
                     /locus_tag="PLY81_05155"
     CDS             complement(5183..>5421)
                     /locus_tag="PLY81_05155"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNF29646.1"
                     /translation="KLPEKDEIKNMGKNQAGIALKTFYNDNNIKGFDLTSQREMSGTM
                     YIAGKLKNNNKGYNITVMAKKTNDKFGIISIRIN"
BASE COUNT         2053 a          723 c          916 g         1729 t
ORIGIN      
        1 agaagaacaa aaacaaaaat atattcctaa attggcaagt ggcgaattta aaggagctta
       61 tggacttaca gaacccaata gtggcagcga tgcactgagt gcaaaaacta cagccaaact
      121 aagtgaagat ggaaaatatt atattttaaa tgggcaaaaa tgttggatta ctaatggggg
      181 ctttgcagat gtttacacag tatttgcaaa agtagatgga acacagttta cagcatttat
      241 tattgaaaga ggttttgaag gatttacgca aggaccagaa gaacacaaaa tgggtattaa
      301 aggatcatca actgttcaat tatattttca agattgtaaa gtacccgtag aaaatttatt
      361 aggagaaatt ggcaaaggcc acattattgc gtttaatatt ttaaatattg gaaggttaaa
      421 attatgtgct gcagcattag gtggtagtaa aagtgctgca acaacctctg ttcaatatgc
      481 taataccaga gagcaattta aattgcccat tgcaaaattt ggtgcaataa aacataagtt
      541 ggcagaaatg gcaataaaaa tttgggttac agaaactgca ttatacagaa ctgcaaaatg
      601 gatagacaat aaagagcatg aattattaga agccggaaaa ccttttaatg aagcattatt
      661 aggagctgca gaagaatatg ctattgaatg tgcaatatta aaagtatttg gtagtgaaac
      721 attagatttt gtagtagatg aaggagtgca aattcatgga ggcaatggct ttagcgatga
      781 gtatgtaata agtaaggctt atagagatag cagaataaat agaatttatg aaggcactaa
      841 tgaaataaat agattactta ccgttgatat ggtattaaaa cgtgcaatga aaggcaaatt
      901 agatttaatg acacctgcta tgaacgtaca aaaagaatta atgagcattc cagattttgg
      961 cactgaggag gatggagctt ttagcaaaga attaaaatat attgccaact tcaaaaaagc
     1021 aattttaatg gttgctggtg cagcagttca gaagttaatg atgcagttag ataaagaaca
     1081 agaagtattg atgaatattg ctgatatggc tattgaagtt tttcattcag aaagtgcctt
     1141 attaagggtt ataaagctat ataatcaaca tggtgcagag gcaacatctc tacagactga
     1201 tatgatgaga acttatttat atgatgctgc cgataaaatt aataaagcag gaaaagatgc
     1261 tctaaacagt tttgcagatg gtgatgaatt aagaatgatg catattggat taaaaaggtt
     1321 tacaaaagtt gaacctttta acaccaaaga agcaagaaga agaatttgca ataaaatgtt
     1381 agaagccaat gcctattgtt attaggcctc aaaagggtaa attttatcac tttaaattta
     1441 cccttttgag taacatatta aatttaatta tcttaatttt gaaataagtt ttttaaaaag
     1501 taaaatatta ttagattgta agctaaaaac tatttaataa atattaagct atttgcaatt
     1561 aaagataatt ggttgcattt taaacaagca attaatgtat tttaattcat taaaacctat
     1621 aaatgcactt taaaaagaat agtcatatca tttctattag tttcttgtat ccaattctga
     1681 tattgtttgt tatttcttgt aattctaatt ctaaaaataa acttaattca gaacggttac
     1741 ctgcaataga tactattatt gataaagctg gaaaaatttc ttttttaggt ggcgaacctg
     1801 ccttgaaatt tattgattct gctacttaca acaaaaaatt ttctacaaaa gaaaagataa
     1861 gactgtttgg ctatctttct tatttatact ctaaatattt acataatatt gaaaagtctg
     1921 ctttgtactc cgattctatg ttttatcttc taaacaataa aaacatagat gattattcct
     1981 atgaatattc ttattcttat tttacacgag gagatgtttt atttgatcaa agaaagtttg
     2041 gagaagcata ctcttactac tataaaggca aacaagtagg tgttgcattt tatgactctg
     2101 caaccttagg agagtatagc tataggttag ggcatgtatt gtatttgcaa ggtaaattta
     2161 atgaagctaa gctaagtttt atgcaggctt tggaattatg gaaaacagcc acattaacat
     2221 ttggaaaata ctatgctacg caagaaatta ttagcaatat tggtttgtgt tattataaag
     2281 caaaccaacc agatagttca ctattttact ttaaaaaggc actaaactat atagataatg
     2341 cagagcaaaa ttatattaat agaaaggaat tacataatgc tgctaaaggg gtaatttttg
     2401 gtaatattgg taaagtgtac gaaaaaacta aacagtatga ttcggcatta acgtattata
     2461 aaaaaggagt aataattaat ttagcagatg gttatgaaat agaagatgca ttaataaata
     2521 atattcatgt tgcacaagtt tatggcatac agcataaata tgatagtatg aaaaacacat
     2581 tactgcaaac tgaaaaatgg ctaaaaaaat cttcagaata taagttagcc aatttaatgt
     2641 ggcataaaca aatgtggcaa tattatgata aaacaaatca aacccaacaa gcctatactt
     2701 acttaacaaa atacacagaa ttatttactc agcactctaa agaaaccatt tcaattaact
     2761 ccatcaatat tgatcagcaa gtagaatttc tagaatctaa aaaggcaata gaagtaatgc
     2821 aaaaagaatc gaagattaaa gaaatttatt tatatggatc cattttacta gttgttttaa
     2881 tgagtggctt tattgtggtt actttaattt tttggaacaa gtcgaagcaa aatattaaga
     2941 aactacagca gcttaataat actattcagc atcaaaattt tattttagaa acacaaagca
     3001 gagaaaagga aaaaatttta aaattagttg cacacgattt aagaacacca attgcatcca
     3061 ttgctgcatt aacagatatg ttagaagagg aaaaagaaaa agaacctcaa aaagagttga
     3121 ttggaattat gaaaatggca tgtgatagtt ctttaaagtt aatttctgaa attttagaaa
     3181 gctcttcaag cacaaacaaa gaaattgaga aagaaatggt aagtgccagt gattgtttat
     3241 cagattgtat ttctcaatta caaattaatg ctgcagaaaa gaaacaaaaa tttgttataa
     3301 agcctttgcc ttacgcagtt agcttgtacg ttaataaaga taaaatgaaa agggtaatgt
     3361 ataacctaat taccaatgct attaaattta gccacgaggg aggtgaaata catttaaaaa
     3421 tggagcagca taatgataaa tctattttaa tttctattca agattttggt attggcatac
     3481 ccgatcatat taaaactaat atttttaatt taaatgatga agcaaaaaga agtggtacta
     3541 aaggcgaaaa atcttttggt ttaggtttaa ctatctgtaa agatattgtg gctgcacata
     3601 atggtaaaat atgggttgaa agtactgaaa ataaaggcac aacatttttt gtagaattgc
     3661 ctttaagtta atgtaataat ttacttacac caagtacgga ttgaaatttt cataaaaatt
     3721 acatttccca ttttggtaac ttttgcttac ttttgacaaa acaagaaggt tgagagtaat
     3781 tgcaaagaaa gtacttagag atttttggga gaaacactgt gattgtgagc aacaattaaa
     3841 agcatggttt ctggaaacct ctaaagccga atggataaat cccaacgaaa taaaagttga
     3901 gtatccgagt gcaagcatta ttggcaacga caggattgta ttcaatataa aaggcaacaa
     3961 gtatcgctta atagtaaaaa tcaattttga ttatcaaatg gtatggatac ggtttattgg
     4021 gacacattcc gaatatgaca aagtaaacgc aaaaactatt taatatgaac atcaagccca
     4081 tcaagacaaa gaaggactac gaacaagcaa tgctaaggct tgagaaacta tttgacgcca
     4141 agaaaggaac atccaagggg gacgaattag aagtaataag tatgttagtt gaaaaatacg
     4201 aagatgaaca tttcccaata gacctgccag acccagttga agctataaaa tttagaatgg
     4261 agcaacttgg aatgacacaa acagacttag ctaatcttgt tgggcaaaaa agtcgtgcaa
     4321 gtgaaatatt gaatcggaag cgaaaactct cgttagatat gattagacag ttgcatcaac
     4381 agtggcgtat acctacagag gttttggtgc aagcttacta attacaacgt acttctgcca
     4441 acgttgcatt ggtaatatgg tgggtgaagt gtatcgtcct aaaattagtt gacaattttt
     4501 caatgtttgt actaccatcg gtttacagta atttttgtaa acctgatagt agtttacact
     4561 ttgttctttc tcaaacttaa tgtaataatt tacttacacc aattttacca aaacataaat
     4621 atttactaat tcactgtttt ttacagcagc atttttttga ataccttcta aataaaaacc
     4681 tgctttttgt aaggctttca tactggcttt attattttca aaaactcttg caaaaattct
     4741 ttttactcca ggcctatcat ctttaatatg ctgtacaatt ttttcaatag ctttggtagc
     4801 aatacccaaa ccccaaaatg gttcaccaat aaaataacct aactctatac tgcatttata
     4861 aatatcatcg taagttaata aaccaatacc accaacaatt ttatggttac attcaatagc
     4921 aaattttgaa acgggtatta tagcactttc tctatttatc cattgcaaag catctgttac
     4981 agtataaggg ctaggaaaat tatccaatac attattccaa atatttttat tattggcaat
     5041 agcagccagg gcttgagcat cttctttttt ccaaggtctt aaaataatat tatccatgat
     5101 tgatgttagg agtaagcact aaagttaatc aaaaatataa aagataatat ttgtaaaaaa
     5161 gaagtagttt atgagagaga aattagttta ttctaataga tataatacca aatttatcat
     5221 ttgttttttt ggccattaca gtaatattat agcccttgtt attatttttt aattttcctg
     5281 caatgtacat agtaccgctc atttctcttt gagaggttag gtcgaatcct ttaatattgt
     5341 tatcgttata aaaagttttt aaagcaatac cagcttggtt tttacccata ttttttattt
     5401 cgtctttttc gggtagtttt a
//
LOCUS       DAOQHP010000121         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8637_length_5366_cov_3.490115, whole genome shotgun sequence.
ACCESSION   DAOQHP010000121 DAOQHP010000000
VERSION     DAOQHP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8637_length_5366_cov_3.490115"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLY81_05160"
     CDS             <1..369
                     /locus_tag="PLY81_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNF29647.1"
                     /translation="DIDGTVNVNCDRCGNTIQQQLWDEFNIIVKMTDEPEIMNEQEED
                     PDIYYISRGESHIHISDWLYEFVSLSVPMQNICGNDENGKSLCNQEVITKLEQMQAKN
                     INAENQMWKGLEQFKDLEKE"
     gene            458..652
                     /gene="rpmF"
                     /locus_tag="PLY81_05165"
     CDS             458..652
                     /gene="rpmF"
                     /locus_tag="PLY81_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNF29648.1"
                     /translation="MPNPKRRHSQQRSAKRRTHYIAESVTLSKDTATGETHVRHRAHV
                     SEGKLYYKGKLVAEKAPLKA"
     gene            765..1709
                     /gene="plsX"
                     /locus_tag="PLY81_05170"
     CDS             765..1709
                     /gene="plsX"
                     /locus_tag="PLY81_05170"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNF29649.1"
                     /translation="MNIGFDLMGGDFAPTEAVKGLKLYLQQANNVTVIGIANKAIIEP
                     LLAEHSIQNNIQLIHSDEVIGYNEHPTKALKEKPNSSIAIGFHLLATGKIDAFISAGN
                     TGAMLVGAMFSIKAIAGVQRPTIATIIPKTNGTTGLLLDVGLNADCKPEHLNQFATLG
                     NLYAQNILGIPNPTTGLLNVGEEEGKGNILAQATYPMLKENTMINFVGNVEGRDIFND
                     KADVIVCDGFTGNVVLKMAESFYDIATQRNLHNDTYLSRFHYENYGGTPVLGVSKPVI
                     IGHGISNDVAFKNMLALAEKMITSDVCGKIKNSFSTNN"
     gene            1746..1973
                     /locus_tag="PLY81_05175"
     CDS             1746..1973
                     /locus_tag="PLY81_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29650.1"
                     /translation="MTIENFKSLPIEKKLIELKHSAELLGSYERNSENGGPKTPGDIY
                     ALYDFWVFLSDDEKMLIPTRRNPFPEVEEEE"
     gene            2206..3042
                     /locus_tag="PLY81_05180"
     CDS             2206..3042
                     /locus_tag="PLY81_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF29651.1"
                     /translation="MFKLFSTGYLLLSTSLIFTLSSCGNSKNEKVSNTDSLNNVQAKP
                     TPGTPIKYDSSKRYIFLTWDDSPQPPGTTNCLKIFKEEGVKSTFFAVGFNQVGPHKKR
                     IIDSIRNSYPNVLLANHSFSHGFNDKYTKFYNTPDSAYNDFMRNERDLNIGVKIIRLP
                     GNNTWAFNGKIHGQKADNAIVKKLDANGYKIIGWDLEWMQQPKAKAPKQSVEEMIKSI
                     NQKFDEGMTNEANMLVILSHDRLFEKQQYADSLRKFIQVLKSDPRNVFETIDHYPLVQ
                     NK"
     gene            3055..4071
                     /locus_tag="PLY81_05185"
     CDS             3055..4071
                     /locus_tag="PLY81_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNF29652.1"
                     /translation="MIDFRSDTVTKPTKQMLEAMLQAKVGDDVFGEDPTVNELEEYAA
                     NLFGIQAALFCPSGTMTNQIAIKVHTQPGDEIICDNLSHIYQYEGGGIAVNSACSVKL
                     INGNYGKINAKQVAENINNINDVHKPISKLLSLENTSNRGGGSCYHFDDFIAIKEVCT
                     QNNLMLHLDGARLFNAIIYKNESAKQYGKVFNSISICLSKGLGAPIGSLLLGDKNFIL
                     KARRVRKVFGGGMRQAGIIAAAGLYALQNNIQRLSTDHHHAQLIATALRQKSFVTNIL
                     PVETNIIIFEVAEKYTAKELIATLYKNNILAFAIAENQIRLVLHLGITTEMVEETIQL
                     IQKL"
     gene            4088..5293
                     /gene="lysA"
                     /locus_tag="PLY81_05190"
     CDS             4088..5293
                     /gene="lysA"
                     /locus_tag="PLY81_05190"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNF29653.1"
                     /translation="MQQPTNEQFLEIAQQFGTPVYVYDANKIVSQYQKFKSAFNSPTV
                     KIFYACKALTNINILKLLKLQGANIDCSSINEVKLAIHAGFEPQQILYTSNGIGFDEI
                     EEAKNLGIHINIDSISNLKKFGQKFNNSYPVGVRLRPNIMAGGNIKIATGHDKSKFGI
                     PITQIAEVVDIMQQYNLLINGVHIHTGSDIKEADVFLKGIEVLFDVIHLFPNLMYVDL
                     GGGFKVPFEDTEEETDMITLANKILEKFNQHLHQNKLQIWFEPGKYLVSQAGYFLTKI
                     NVLKKTNNIEFASINSGFNHLLRPMFYDAYHKIVNISNPNGTIKEYDIVGNICETDTF
                     AANRNINEINEGDLLVFYNAGAYGFEMSSNYNSRYKPAEVLIHNNQISLIRKADRFED
                     LLKNQIVVL"
BASE COUNT         2059 a          745 c          885 g         1677 t
ORIGIN      
        1 gatattgatg gaacagtaaa tgtaaattgt gacagatgtg gtaacaccat acaacaacaa
       61 ttatgggatg agtttaatat tattgtaaaa atgactgatg agccagagat aatgaatgag
      121 caggaagaag acccggatat ttattatata agcagaggtg aaagtcatat tcatatttcc
      181 gattggttat atgagtttgt aagtttaagt gtgccaatgc aaaatatttg cggaaatgac
      241 gaaaatggca aatcattatg taatcaagag gttattacta aacttgagca aatgcaagca
      301 aagaatatta atgcagaaaa ccagatgtgg aaaggattag aacagtttaa agatttagaa
      361 aaagaataag tacaaaataa tataaaagct actacaaata acttttatta tttaaaaaca
      421 aataaaaaca attggattaa ttttaaaaat ttgagttatg ccaaatccaa aaagaagaca
      481 ctcacaacaa cgtagtgcaa aaagaagaac acattatatt gctgaatcag ttactttgag
      541 taaagatact gcaacaggag aaacacatgt acgccaccgt gctcatgtaa gtgaaggaaa
      601 attgtattat aaaggaaaat tagttgcaga aaaagctcct ttaaaagctt agcaactact
      661 atgttgaatt ctttaaataa tttaagtgtc aggtacttta gtggtatcag acacttgttg
      721 ttttatagaa gttagtacaa tttaccttta aatctaatgg aagcatgaat attggttttg
      781 atttaatggg tggtgatttt gcaccaaccg aagcagttaa agggcttaaa ctatacttgc
      841 aacaagcaaa taatgttact gtaattggca ttgctaataa agccattatt gaacctctat
      901 tagcagaaca cagtattcaa aacaatatac aattaataca ttcagatgaa gtaattgggt
      961 acaatgaaca tcctacaaaa gctttaaaag aaaagcccaa tagctctatt gcaattggtt
     1021 ttcacttatt agcaaccggt aaaatagatg cttttattag tgctggcaat actggtgcaa
     1081 tgttagttgg tgctatgttt agcattaagg caattgcagg agttcaaaga ccaacaattg
     1141 caactattat tccaaaaaca aatggcacta caggtttatt attagatgtt ggtttaaatg
     1201 cagattgtaa gccagaacac ttaaatcaat ttgctacctt aggtaatctt tatgctcaaa
     1261 atattttagg cattcccaat cctacaacag gtttattaaa tgttggcgaa gaagaaggca
     1321 agggtaatat acttgcacaa gccacctacc ctatgcttaa agaaaataca atgattaatt
     1381 ttgtgggaaa tgtggaaggg agagatattt ttaatgataa agcagatgta atagtttgtg
     1441 atggttttac aggaaatgta gtattaaaaa tggccgaaag tttttatgat attgctacac
     1501 aaagaaactt acataacgac acttatttaa gtaggtttca ttatgaaaat tatggcggta
     1561 cacctgtatt gggtgtttca aaaccagtaa taattggcca tggtattagc aatgatgtag
     1621 cttttaaaaa tatgttggca cttgcagaaa aaatgattac ttccgatgtt tgtggtaaaa
     1681 ttaaaaacag cttttcaacc aacaactaaa caaaacttaa aattattaac ctatatactt
     1741 agtttatgac aattgaaaat tttaaatcat tacccataga aaaaaaatta attgagttaa
     1801 aacactccgc cgaattgtta ggatcttacg aaagaaattc tgaaaatggt ggacctaaaa
     1861 caccaggtga tatttatgct ttatacgatt tttgggtatt cttaagcgat gatgaaaaaa
     1921 tgttgattcc tactagaaga aacccttttc ctgaagtaga agaagaagaa taattaatat
     1981 ttctatcatt taattatagt gggtataact tgcaagttgc tggttatacc ttttttatgt
     2041 gcccaaaaaa atttcatcat ttcttctttt aatttcctat caatttataa ttaataatct
     2101 tactcatagt ataatttaca tatttacaaa acttattcac taggtattaa taacattaat
     2161 ttttgctact taatatgatt tttatatttg aacaaaataa attctatgtt caaattattt
     2221 tctacaggtt atctgctact ttctacttcg ttaattttta cgttaagtag ttgtggaaat
     2281 tcaaaaaatg aaaaagtatc caatacagat tctttaaaca atgtacaagc caaacctaca
     2341 cctggaactc caataaaata cgatagcagt aaacgctaca tttttttaac ttgggatgat
     2401 tctcctcaac caccaggtac aaccaattgt ttaaaaatat ttaaggaaga aggtgtgaaa
     2461 tctacttttt ttgcagtagg ctttaaccaa gttggccctc acaaaaaacg tatcatagat
     2521 tctatacgaa acagctaccc aaatgtactg ttagccaatc atagtttctc tcatggcttt
     2581 aatgataagt acaccaaatt ttataacact ccagatagtg cttacaatga ttttatgcgt
     2641 aatgaaagag atttaaacat tggagtaaaa ataataagac tgccaggcaa taacacatgg
     2701 gcatttaatg gcaaaataca tgggcaaaaa gcagacaatg caatagtaaa aaaattagat
     2761 gctaatggtt ataaaattat aggctgggat ttagagtgga tgcaacaacc aaaagcaaaa
     2821 gcacccaaac aaagtgtaga ggaaatgatt aaaagcatca accaaaagtt tgatgaaggc
     2881 atgactaatg aagcaaatat gttggttatt ttatctcatg atagactatt tgagaagcaa
     2941 caatatgcag actctttaag aaaatttatt caagttttaa aaagtgatcc tagaaatgta
     3001 tttgaaacaa tagaccacta tccattggtt caaaacaaat aaaatatatt taggatgatt
     3061 gattttagga gcgatacagt taccaaacca actaagcaaa tgctggaagc aatgcttcaa
     3121 gcaaaagttg gagacgatgt ttttggcgaa gacccaacag taaacgaatt agaagaatat
     3181 gctgctaatt tatttggtat tcaagcagca ttgttttgtc catcaggaac aatgaccaac
     3241 caaattgcta taaaagttca tacccaacca ggagatgaaa ttatttgcga taacttaagt
     3301 catatttatc aatatgaagg tggaggtatt gcagtaaata gtgcttgttc tgtaaaacta
     3361 attaatggta attatggtaa aattaatgct aagcaagtag cagaaaatat taacaatata
     3421 aatgatgttc ataagccaat aagcaaattg ctgagtctag aaaatacttc caatcgtggt
     3481 ggcggtagtt gctaccattt tgatgatttt attgcaataa aagaagtatg tactcaaaat
     3541 aatttaatgc tgcatttaga tggtgcaaga ttgtttaatg caataatcta taaaaatgaa
     3601 tcagcaaaac aatatggcaa agtatttaac tctatttcta tatgcctaag caaaggattg
     3661 ggtgccccaa taggcagcct attattaggc gataaaaatt ttattttaaa agcccgtaga
     3721 gtaagaaagg tttttggagg aggaatgaga caagcaggta taattgcagc agcaggctta
     3781 tatgctttac aaaataatat acaaagactt tctacagacc accatcatgc tcagcttatt
     3841 gccacagcat taaggcaaaa aagctttgta actaatattt taccagtaga aaccaatatt
     3901 atcatatttg aagtagctga aaaatatact gcaaaagagt taatagccac tctttataaa
     3961 aataatattt tagcttttgc catagcagaa aatcaaatta gattagtgtt acatttaggc
     4021 ataacaactg aaatggttga agaaaccata caattaattc aaaaattata atacttattt
     4081 ttgcaaaatg caacaaccca ctaacgagca gtttttagag attgctcaac aatttggtac
     4141 accagtttat gtatatgatg ctaataaaat tgtaagtcaa tatcaaaaat ttaaatctgc
     4201 ttttaacagc cctactgtaa aaatatttta tgcctgtaag gcactaacta atatcaatat
     4261 tttaaaactt ttaaaacttc aaggtgccaa tattgattgt agcagcatta atgaagtaaa
     4321 gcttgcaata catgctggtt ttgagcccca acaaatacta tatacatcta acggaattgg
     4381 gtttgatgaa atagaagaag ccaaaaacct tggtattcac ataaacattg atagcataag
     4441 taatttaaaa aagtttggac aaaaatttaa taactcctac cctgttggtg ttaggttaag
     4501 gcccaatata atggctggag gaaatataaa aattgctaca ggtcatgata aaagcaaatt
     4561 tggaattcct ataacccaaa ttgccgaagt agtggatata atgcagcaat ataatttatt
     4621 aattaatggt gtgcatatac atacaggaag cgatattaaa gaagcagatg tttttttaaa
     4681 aggtattgaa gtgttgtttg atgtaatcca tttatttcct aatttaatgt atgttgattt
     4741 aggcggtggc tttaaagttc cttttgaaga tacggaagaa gaaactgata tgatcacatt
     4801 agcaaataaa attcttgaaa aattcaatca acacttacat caaaataaat tacaaatttg
     4861 gtttgaacct ggaaaatatt tagtgagcca agccggatat tttttaacaa aaattaatgt
     4921 gctcaaaaaa accaataata ttgaatttgc aagtatcaat tctgggttta atcatttatt
     4981 acgccctatg ttttacgatg cataccataa aattgtaaat attagtaacc ccaatggcac
     5041 tattaaagaa tacgatattg ttggaaatat ttgcgaaaca gacacatttg ctgcaaacag
     5101 aaatataaac gaaattaacg agggtgattt attggttttt tataatgccg gagcttatgg
     5161 ttttgaaatg agtagcaatt ataatagtag atataaacca gcagaagttt taattcataa
     5221 caatcaaatt agtttaatta gaaaagcaga taggtttgaa gatttattaa aaaatcagat
     5281 tgttgtttta taaaaaatct aactaaatta tcatcttaac aatattaatt tttttcctgt
     5341 tctattcaat atttgtaata taaatt
//
LOCUS       DAOQHP010000122         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8666_length_5354_cov_3.534063, whole genome shotgun sequence.
ACCESSION   DAOQHP010000122 DAOQHP010000000
VERSION     DAOQHP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8666_length_5354_cov_3.534063"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLY81_05195"
     CDS             <1..520
                     /locus_tag="PLY81_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF29654.1"
                     /translation="ESTKYPFVIRYPRGEGVMPEWKTAFEQLTIGKGRKLKDGENIAI
                     LTFGHPGNFAQAAIRELRTNGINPAHYDLRFVKPIDEDLLHEVFTNYNKVITVEDGTV
                     VGGFGSAVVEFMAAHHYNAEIKILGIPDRIVEHGTLKELHRECEYDTEAIVEAVKNMS
                     NIKVKTEQMLMG"
     gene            complement(684..1370)
                     /locus_tag="PLY81_05200"
     CDS             complement(684..1370)
                     /locus_tag="PLY81_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29655.1"
                     /translation="MLDVNVKKILIADDEPDIIEIIQYNLLKEGYIVYTAKNGDEAID
                     KAKKINPDLIILDIMMPKKTGIEVCTILRTQAMFKETMVVFLTALSDEETHIKGLEIG
                     ADDFISKPISSKVLVSRINALFRRISKEEVKAINIGNLTIDPLKFIVLKNGNEIILAK
                     KEFELLYLLASDIGKVFLRNEILQHVWGANVIVGDRTIDVHVRKIRQKLNLNCITTVK
                     GVGYKFVIEA"
     gene            1523..3712
                     /locus_tag="PLY81_05205"
     CDS             1523..3712
                     /locus_tag="PLY81_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29656.1"
                     /translation="MKLLVSILLFITTGFSLAAQSFVPTVPIPMPQVPTMRQLNHDII
                     DKSQKLITSLDGKDDGVFTVSVSDSLNQITNDALQIIVDNMQAEIELNKLIDENNKYK
                     WLRSLNDMLSSFISGYRVKLFKGNILYNLIISYKEAIELEWKNESIKPIVERNIFEIG
                     EILIKNFALKNNVGVAESEDIILLKRIERYPHRTMSLLSQNPNAYFADSVIVSQAFKD
                     PEQLYNYAAAPNALGRRIQASNNPLVKRIGLLALMKTGRMYFPFLDNLYREKISMDSI
                     TKMLHDSLGYFKLLVTTQIDYAKRLKQNDTPLVMNVLTNKLKSKAVELYVNQINALHD
                     AQENIRFECLKQLSPQDLYYVAVLGEEEIYTSSYLGVYKRIFLKMKHPVADTLLKWVD
                     YDYYKKFIKIAANYNTLDDFLTRMNKANAEKLMRNFVTGLEKTKTLEDAVDVANSYAS
                     IYNNNIKKLILNQIEQNLLQSEKEQNKRGQIIYSLLNTIFLSMDSSQKIDLASRLGIN
                     GIYKMPISSLKDSTGRIIIQQFFYGDKDGKSVFNSFFKYYHAPLWKKVEKENWVELYS
                     TKGVPITIYCNKPLDNELDLDLKAQEALGRYLEENELYPTVVIHRGHSYFVGSTIDQL
                     PSSAKVVLLGSCGGYQRLSDVLETCPESHIISSKQTGTGTVNIGLINAITHDLRLNKD
                     LNWPLIWKNLAKQFVGVSKERFDDYVPPHKNLGAIFIIAYNLALQVE"
     gene            complement(3726..4862)
                     /locus_tag="PLY81_05210"
     CDS             complement(3726..4862)
                     /locus_tag="PLY81_05210"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNF29657.1"
                     /translation="MTKILVVGAGMVGSAMALDLSKKYAVTVADINSTNLNKIQQKNN
                     AIQIISLDVTDETALTNCIVNFNFVVCAVPGFLGFKTLRTIINTGKSVVDISFFPENA
                     LELDALAKQKNVTAIVDCGVAPGMGNIIVGYQNTQMQINNFECLVGGLPKIKKWPFNY
                     KAPFSPIDVIEEYTRPARYIENGNLVVKPAMSDIEHIEMDGVGTLEAFNSDGLRSLIY
                     TMPHITNMKEKTLRYPGHIEKIIALRDAGFFNEEPIIINNTKISPLEFTSKILFKEWL
                     LGEEEEEITVMKIIISGTQNDQPIKVEYNLYDEYNATTKTSSMARTTGYTATAAVCLI
                     VENLFVEKGIFPPELVGKNAVCYNYIINYLLERGVKYKQEVTIG"
     gene            complement(4950..>5354)
                     /locus_tag="PLY81_05215"
     CDS             complement(4950..>5354)
                     /locus_tag="PLY81_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF29658.1"
                     /translation="IMNIRKRLRQKPDLHTSALNDILFILLLFFLIVSTLANPNVVKV
                     NNPKGKSDTKAKQNIVISIDKENKIYLGQAVIPENNLDSVIKVEVDKVKAFIDTPSVV
                     INADTISYTGGFFKVMGAAKKAGAKIVANVKD"
BASE COUNT         1916 a          789 c          763 g         1886 t
ORIGIN      
        1 agaatctaca aaatatcctt ttgtaattcg ttatccaaga ggcgaaggtg ttatgccaga
       61 atggaaaact gcttttgaac agttaaccat tggaaaagga agaaagctaa aagatggaga
      121 gaatattgct attttaactt ttggtcatcc tggcaacttt gcacaagctg caataagaga
      181 acttagaact aatggtatta atcctgcaca ttacgatttg cgttttgtaa agccaattga
      241 tgaagatttg ttacacgaag tttttacaaa ctacaataaa gttattactg tggaagatgg
      301 tactgttgtt ggtggttttg gcagtgcagt tgtagaattt atggctgctc atcattataa
      361 tgctgaaata aaaatattag gcattcctga taggattgtt gagcatggaa ctttaaaaga
      421 actacataga gagtgtgaat atgatactga agcaattgtt gaggctgtaa aaaatatgag
      481 taatattaaa gtaaaaactg agcaaatgct catgggataa tactttgcca atgttttgca
      541 actttcattg cagtgtatct tacaatttag ctttgataaa ttacaaaaca taatttaata
      601 attgtagctt gatttaataa atgaagttat aatgttgttt taatagtgta acaaactctc
      661 aaggcaccaa tttacggtta gatttacgct tcaattacaa atttataacc cacccccttt
      721 actgtagtaa tacaatttaa gtttaatttt tgtctaattt ttcttacatg cacatctatt
      781 gttctatcac caacaattac gtttgcaccc catacatgtt gtagtatttc gtttcttaaa
      841 aaaactttac caatatctga tgctaataga tatagaagtt caaattcttt tttagccaat
      901 ataatttcat taccattttt tagaacaata aattttaatg ggtctattgt taagttgcca
      961 atatttattg ctttaacttc ttccttacta attcttctaa acaaagcatt tattctacta
     1021 actaaaactt tagagctaat tggtttacta ataaaatcat ctgctccaat ttctaaacct
     1081 ttaatatgtg tttcttcatc gcttaaagct gttaaaaaaa caaccattgt ttctttaaac
     1141 atagcttggg ttcttaaaat agtacaaact tcaatacctg tttttttagg catcattatg
     1201 tctaaaataa ttaaatcggg gtttattttt ttggctttat ctattgcttc atctccattt
     1261 ttagcagtat aaacaatgta gccttctttt aataaattat attgtattat ttcaataata
     1321 tctggttcat catctgcaat taatattttt tttacattta catctagcat aatttctacg
     1381 caatttaatg ctacaaaaat aatcttctaa catgtataac agcattatta atattaccta
     1441 attgttattt gtcattaaca acttatatag aaataggcaa gtataaaata tttacttttg
     1501 tgaaacatta gagcaaatta gaatgaaatt attagtttcc attttacttt ttataacaac
     1561 aggattttct ttagctgcac aaagttttgt accaactgta ccaataccaa tgccacaagt
     1621 accaaccatg cgtcagttaa atcatgatat tattgataaa agccaaaaac ttataacaag
     1681 tttagatgga aaagatgatg gagtttttac tgtaagtgtt agtgattctt taaaccaaat
     1741 tactaatgat gctttgcaaa ttatagtaga taatatgcag gccgaaattg aattaaataa
     1801 attaattgat gaaaataaca aatacaaatg gctacgcagt ttaaacgata tgctctcctc
     1861 ctttatatct ggctatagag taaaactttt taaaggaaat attttataca atttaattat
     1921 tagttataaa gaagcaattg aacttgaatg gaaaaatgaa tcgataaaac caattgttga
     1981 aagaaatatt tttgaaattg gtgaaatact aattaaaaat tttgcactaa aaaataatgt
     2041 tggtgttgca gagagtgaag atattatttt attaaaaaga atagaaagat accctcatag
     2101 aacaatgagt ttactatcgc aaaacccaaa tgcatatttt gcagatagtg ttattgtaag
     2161 ccaggctttt aaagaccctg agcaactata caactatgca gcagcaccaa atgctttagg
     2221 ccgaagaata caagcatcta acaacccctt ggttaaaaga attggtttat tggctttaat
     2281 gaaaaccggc agaatgtatt ttcctttttt agataattta tatcgagaaa aaatttcgat
     2341 ggactcaatt actaaaatgt tgcacgacag tttaggctac tttaaacttt tagtaaccac
     2401 acaaattgat tatgcaaaaa gattaaaaca aaatgataca ccattggtaa tgaatgtgct
     2461 taccaataaa ttaaaatcta aagctgttga attatatgtt aatcaaataa atgctttaca
     2521 cgatgctcaa gaaaacataa gatttgaatg cctaaaacaa ctatcgccac aagatttata
     2581 ctatgttgca gttttaggcg aagaagaaat ttatactagc agttacctgg gtgtttataa
     2641 aagaattttc ttaaaaatga aacacccggt ggcagatact ttattaaaat gggtggatta
     2701 tgattattat aaaaagttta taaaaattgc cgctaactat aatacactag acgatttttt
     2761 aacaagaatg aataaagcca atgcagaaaa gttaatgaga aactttgtaa caggtttaga
     2821 aaaaactaaa acacttgaag atgctgtaga tgttgctaac tcttatgcaa gcatttataa
     2881 taataatatc aagaaattaa ttttaaatca aatagaacaa aaccttttac aaagcgaaaa
     2941 agaacaaaac aaacgtgggc aaattatata cagtttacta aataccattt tcttatctat
     3001 ggattcatct caaaaaatag atttagccag tagattagga attaatggaa tttacaaaat
     3061 gccaattagt agtttaaaag acagcactgg cagaataatt attcaacaat ttttttatgg
     3121 cgataaagat ggaaaaagtg tttttaattc tttctttaaa tattaccatg cacccttatg
     3181 gaaaaaagta gaaaaagaaa actgggtaga actatatagc acaaaaggag ttccaataac
     3241 tatttattgc aataagcctt tagataatga actagattta gatttaaaag cacaagaagc
     3301 tttaggaaga tatttagaag aaaatgaact gtacccaaca gttgtaatac acaggggcca
     3361 tagttatttt gttgggtcaa caattgatca actcccaagc tcggcaaaag tagttttatt
     3421 aggaagttgt ggtggttatc aaagattaag tgatgtatta gaaacttgcc ctgaatctca
     3481 tatcatttca tctaaacaaa ctggtactgg tacagtaaac ataggtttaa ttaatgccat
     3541 tacacatgat ttaaggctta ataaagattt aaattggccc ttaatttgga aaaatttagc
     3601 aaaacaattt gtaggtgtta gtaaagaaag gtttgatgat tatgtaccac cacataaaaa
     3661 tttaggtgct atttttatca ttgcttataa tttggcttta caagttgaat aaaatttaaa
     3721 aatgtttatc ctatagttac ttcttgttta tacttaacac ctctttctaa taaatagtta
     3781 ataatataat tgtaacaaac tgcattttta cccaccaact ctggtggaaa aatgcctttt
     3841 tctacaaaca aattttctac tattaaacat actgctgctg tagctgtata gcctgttgtt
     3901 cttgccattg acgatgtttt ggttgtagca ttatattcat catataaatt atattctacc
     3961 tttattggtt ggtcattttg tgttccagaa ataataattt tcattactgt tatttcttct
     4021 tcctcctctc ctaacaacca ttctttaaat aatattttag aggtaaactc aagaggtgat
     4081 attttggtgt tgtttataat tatgggttct tcattaaaaa aacctgcatc tcttaaagca
     4141 attatttttt caatatggcc aggataacgc aaagtttttt ctttcatatt ggttatatgt
     4201 ggcatggtat aaattaaact tcttaaccca tcagaattaa atgcttcaag agttccaaca
     4261 ccatccattt caatatgctc tatatccgac atggcaggct ttacaactaa atttccattt
     4321 tcaatatacc ttgcaggtcg tgtatattct tctataacat ctattggcga aaatggagct
     4381 ttgtaattaa atggccattt ttttattttg ggtaaaccgc ctactaaaca ttcaaaatta
     4441 tttatttgca tttgagtatt ttggtagcct acaattatat ttcccattcc tggtgctacg
     4501 ccacaatcaa caattgcagt aacatttttt tgttttgcta aagcatctaa ctctaatgca
     4561 ttttctggaa aaaaagaaat atccacaacg ctttttcctg tatttattat agttctcaaa
     4621 gttttaaatc caagaaaacc tggaacagca caaacaacaa aattaaaatt tacaatacag
     4681 tttgtaagtg cagtttcatc tgtaacatca agtgaaatta tttgaatagc attatttttt
     4741 tgctgaattt tattaagatt ggttgagtta atatctgcaa cggttacagc atatttttta
     4801 gataaatcta aagccattgc actgccaacc atacctgcac caacaactaa aattttcgtc
     4861 ataattatat tttgtaactt gttcaagtta gcaaaattat taatagcttg aaggtttata
     4921 gaaaaagaaa ttggagtaaa taactaaaat taatctttta cgttagcaac aattttagca
     4981 cctgcctttt tagctgctcc cattacttta aaaaaaccac ctgtataaga aattgtatca
     5041 gcattaatta caacagaagg agtatcaata aatgctttta ctttatctac ttcaactttt
     5101 attactgagt ctaaattatt ttcaggtata actgcctggc ctaaataaat tttattttcc
     5161 ttatcaatac taattacaat attttgttta gcctttgtat cgcttttgcc tttaggattg
     5221 tttactttaa ccacattagg gtttgccaat gttgatacaa ttaaaaaaaa tagtaataga
     5281 ataaacaaaa tatcgtttaa agcactagtg tgtaaatctg gtttctgtct taatcgtttt
     5341 ctgatattca taat
//
LOCUS       DAOQHP010000123         5330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8736_length_5330_cov_3.892701, whole genome shotgun sequence.
ACCESSION   DAOQHP010000123 DAOQHP010000000
VERSION     DAOQHP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_5330_cov_3.892701"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PLY81_05220"
     CDS             <1..1321
                     /locus_tag="PLY81_05220"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNF29659.1"
                     /translation="NMKHILLIGAGKSATVLIHFLIELVQNKNWHLTVADGNEQLLND
                     KVKTSKNVSILVLKIDNELERKQAIASADIVISMMPPHLHYLIAIDCMEFNKHLLTAS
                     YVDEKIQALAKTIKEKNLLFLCEMGLDPGIDHMSAMKLIHEIKEKGGNIVSFKSHCGG
                     LIAPESDDNPWHYKISWNPKNIILAGKAGATYLENGKVVKLPYEALFKNNISVTTSNN
                     KYAYYPNRDSLNYISIYQLQQIKTFVRTTLRHPQFCTGWNMIINLQLTNEVKQYQTNG
                     LSIAKFFETHFKEFNCHEFLKEIKKNDSLHQQLLFIGLNDKDTIINKGLCNAAEVLQF
                     ILEKKLVLNNADKDLIVMLHEFEYLLHNKKYYVTSLLELAGKDNQQTAMAKTVGLPLG
                     IAAKLILENKITTTGLHIPILPEIYLPVLEELQNYGISFNEDSSEIS"
     gene            complement(1390..3930)
                     /gene="topA"
                     /locus_tag="PLY81_05225"
     CDS             complement(1390..3930)
                     /gene="topA"
                     /locus_tag="PLY81_05225"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNF29660.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGKDFEVKSCFGHIRDLEKDDMGI
                     DINNHYQPRYIVPDEKLKVVNELKKLSKNKEVWLASDEDREGESISWHLAEVLNLNPL
                     ETKRIVFHEITAPAIKKAVANPRFINMDLVNAQQARRVLDRLVGFELSPVLWRKIGMQ
                     RSLSAGRVQSVAVRLIVEREREINNFNTESSYKVEASLTANDINNKAVNFKAESTKKI
                     NNVINATAFLETCKNANFTVKDVQVKPTKRTPSAPFTTSTLQQEASRKLGYGVSKTML
                     LAQKLYESGKITYMRTDSINLSDTALQDIQKQVIESYGNQYYQPRKFKNKNESAQEAH
                     EAIRPTYMSNDTIADDETRKLYELIWKRTIACQMSDAAFEKTTAIINISTNNEELVAT
                     GEVMKFDGFLKVYFEGKDDDEEDNGEEFLPSLKNGQQLVFNEMTATEKFTRAAPRYTE
                     ASLVKKLEELGIGRPSTYAPTISTIIKRNYVEKRDKDAIKRKVDIVTLTPDFKITNTT
                     ILENTGAEKAKLFPTDLGMLVTDFLKQHFKNVMDYDFTAQIEKEFDEIAEGNKKWSSM
                     IDGFYKPFHETIEHTLENAERAKGERELGIEEATGRKIVARMGRYGAMVQIGEATDEE
                     KPRFAKLNPTQSIETITLEEALALFALPRIIGEYNGQELSVNIGRFGPYIKMGDQFIS
                     IPKNEDLFEIDVERAKLFIEEKQQADAPISTYNEMPVTKGKGRFGPFIKWNDMYINVP
                     RKYNFDNLSKQDIEELIEAKINKEANRYIQQWPSEKISIENGRWGAFIRFNKKMLKLG
                     KKADGTKYDTAELETIDIETIKKMIAEQVPNAFAVKEKKLKTKAAKKK"
     gene            4165..5067
                     /locus_tag="PLY81_05230"
     CDS             4165..5067
                     /locus_tag="PLY81_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF29661.1"
                     /translation="MQPTLVILAAGMASRYGSMKQIQGFGPSGETIMEYSIYDAIQAG
                     FGKVVFIIREEFVDMFKENFGKKLEGKIKVDYVYQQLDSYTFGRTIPEERKKPWGTAH
                     AILCCKEKVNEPFAVINADDYYGKNAFVKAHQFLTTVVAENNYAIIGYELKKTLSENG
                     SVSRGVCDVDEEGNLIEINERTKIYKNEKGVITYEDETGLHEVSEAASVSMNFFGFHP
                     NFILTCEKYFSEFLDNEINNPKSEFFIPLVVDKLVKSNTAKVSVIPTTAQWFGVTYKE
                     DAPGVQASINNLIQQGQYPNNLWA"
     gene            5077..>5330
                     /locus_tag="PLY81_05235"
     CDS             5077..>5330
                     /locus_tag="PLY81_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29662.1"
                     /translation="MYKGIAFLYFVCFTLYIIYSRVPDYFDGETAPATIHWLKDSTTN
                     TTIPKAVFIHGKTKYEVDARYVLRSLPENKKLTVIYLVKKP"
BASE COUNT         1802 a          845 c          764 g         1919 t
ORIGIN      
        1 taatatgaaa catattttat taattggtgc aggaaaatct gctactgttt taattcattt
       61 cttaattgaa ttagttcaaa ataaaaattg gcatttaaca gtagccgatg gcaacgaaca
      121 attgttaaat gataaagtta aaacaagtaa aaatgtatct atacttgttt taaaaataga
      181 taatgaactg gaaagaaaac aagcaattgc atctgcagac attgttattt ctatgatgcc
      241 accacatttg cattacttga ttgcaataga ttgtatggag ttcaataaac atctactcac
      301 agcatcttat gtagatgaaa aaattcaggc tttagcaaaa acaattaaag aaaagaattt
      361 acttttttta tgtgaaatgg gcttagaccc tggcatagac cacatgagtg ccatgaaatt
      421 aattcatgaa ataaaagaaa aaggaggaaa tattgtttca tttaaatcgc attgtggagg
      481 tttaattgca ccagaaagcg atgacaaccc ttggcattat aaaataagtt ggaacccgaa
      541 aaatattatt cttgctggta aagctggtgc aacttatctt gaaaatggca aagtggtgaa
      601 attgccatat gaagcacttt ttaaaaacaa tatttctgta acaacaagta ataataaata
      661 tgcttattat ccaaacagag attctttaaa ttatatttca atttatcaat tacagcaaat
      721 taaaactttt gtaagaacca ctttaaggca tccacaattt tgcacaggat ggaatatgat
      781 tattaattta caattaacta atgaagtaaa acaataccaa acaaatggct taagtattgc
      841 aaagtttttt gaaactcatt ttaaagaatt taattgccat gaatttctaa aggaaattaa
      901 aaaaaatgat tcattgcacc aacagctttt atttattggt ttaaatgata aggataccat
      961 cattaataaa ggtttatgta atgctgctga agtactacaa tttattctcg aaaaaaaatt
     1021 agttttaaac aacgcagata aagatttaat tgtaatgctt catgaatttg aatatttgct
     1081 acacaataaa aaatattatg taacaagttt attagaactt gcaggaaaag acaatcagca
     1141 aactgcaatg gccaaaacag ttggtctgcc attaggaata gcagcaaaat taattttaga
     1201 gaataaaata acaacaacag gcttgcatat tccaatatta ccagaaatat atttacctgt
     1261 tttggaggaa ttacagaatt atggaatttc ttttaatgaa gatagttctg aaatttcata
     1321 agtttaataa ttatttaaaa cctattcatt taagccaaaa gaattctatt aataaaaatg
     1381 caataagggc tatttctttt tagcagcttt ggtttttaat tttttttctt taacagcaaa
     1441 agcattaggt acttgttctg caatcatttt cttaattgtt tcaatatcta tagtttctaa
     1501 ttctgcagta tcgtattttg tgccatctgc ttttttacca agtttcaaca tttttttatt
     1561 aaacctaata aaagcacccc atctaccatt ttcaatgcta attttttccg aaggccattg
     1621 ttgaatgtag cggttggctt ctttattaat ttttgcttca atcaactctt caatatcctg
     1681 cttactaagg ttgtcaaaat tatattttct tggaacgtta atatacatat cattccattt
     1741 aataaatggt ccaaatcttc ctttgccttt tgtaacaggc atttcattat aggttgaaat
     1801 tggagcatcg gcctgttgtt tttcttcaat aaataatttg gcacgttcta catcaatttc
     1861 aaataaatct tcatttttag gaatagaaat aaactgatca cccattttaa tgtaagggcc
     1921 aaatctacca atatttacag ataattcctg accattatat tcaccaatta ttcttggtag
     1981 ggcaaataaa gccaatgctt cctctaaagt tatagtttct atgctttgtg tggggtttaa
     2041 ttttgcaaag cgtggttttt cttcatcggt tgcctctcct atttgaacca ttgcaccata
     2101 acgtcccata cgagcaacta tttttctccc tgttgcttct tcaattccta attctctttc
     2161 gccttttgct ctttctgcat tttctaaagt gtgttcaatg gtttcatgaa agggtttata
     2221 aaatccatct atcatactac tccacttctt attaccttct gcaatttcat caaactcttt
     2281 ttctatttgt gcagtaaaat cataatccat tacgtttttg aaatgctgct ttaaaaaatc
     2341 ggtaacaagc attcctaaat ctgtaggaaa aagtttagct ttttcagcac cagtattttc
     2401 taaaattgtt gtgttagtaa ttttaaaatc tggtgtaagt gttacaatat ctacttttct
     2461 tttaatcgca tctttatctc ttttttcaac ataatttctt ttaattatgg tgctaatggt
     2521 tggtgcataa gtacttggac ggccaatacc taactcttct aattttttta ctaaacttgc
     2581 ttccgtatat cttggtgcag ctcttgtaaa tttttctgtt gcagtcattt cattaaaaac
     2641 aagttgctgt ccatttttta atgaaggtaa aaattcttct ccattatctt cttcatcatc
     2701 gtctttaccc tcaaaataaa cctttaaaaa cccatcaaat ttcattactt caccagttgc
     2761 aactagctct tcattgttag tgcttatatt aataatagca gtagtttttt caaatgctgc
     2821 atcactcatt tggcaagcaa ttgttctttt ccaaattagt tcgtacagct ttcttgtttc
     2881 atcatcagca atggtatcat tactcatgta agtaggcctt atagcttcat gtgcctcttg
     2941 tgcagattca tttttatttt taaactttct tggctgataa tattggttgc cataagactc
     3001 tattacttgc ttttgtatat cttgcaatgc ggtatcacta aggttaatac tatctgtacg
     3061 catgtaagta attttaccac tttcatatag cttttgtgcc aataacattg ttttacttac
     3121 cccatatcct aatttccttg aagcttcttg ttgtaatgta gatgttgtaa aaggtgctga
     3181 tggtgtgcgt ttggtaggtt ttacctgcac atcttttact gtaaaatttg catttttaca
     3241 ggtttccaaa aatgcagttg cattaattac attattaatt tttttagtgc tctccgcttt
     3301 aaaattaact gccttattat taatatcatt ggcagttaaa ctagcttcaa ccttataact
     3361 actttctgta ttaaagttat ttatctctct ctctctttca acaattaatc ttacagccac
     3421 actttgtaca cggcctgcac ttaaacttct ttgcatacca attttcctcc acaaaacagg
     3481 gcttagttca aagcctacca atctgtctaa tactctacga gcttgctggg cattcactaa
     3541 atccatatta ataaaacgtg gattggcaac agctttttta atagctggtg cagtaatttc
     3601 gtgaaaaaca attcgtttag tttctaatgg atttagattt aaaacttctg ctaaatgcca
     3661 actaatgctc tctccttctc tatcctcatc ggatgctaac caaacttctt tattttttga
     3721 aagctttttt aactcgttta caactttaag tttttcatca ggtacaatat atcttggttg
     3781 gtaatgatta ttaatatcaa tacccatatc atctttttct aaatctctaa tatggccaaa
     3841 acaacttttc acttcaaaat ctttacccaa tattttttca attgtttttg cttttgccgg
     3901 agactctact attaataaat ttttagccat atcttatact tcctctttca aaatttaaat
     3961 actcaaataa ataatcaaga acaattttag cttaataaaa ttttacttaa actactgcaa
     4021 tattagccat atagctataa aaagcaaaaa cggaagttga tttgataata attaataaat
     4081 attttttatg aagaaatcta attatagaag gtataaatta ttctatatta aacaattatc
     4141 ttcgcagcat tttaaattaa aattatgcaa cctacattag taattttagc tgcaggaatg
     4201 gcaagccgtt atggcagtat gaaacaaata caaggttttg gaccaagtgg tgaaacaatt
     4261 atggaataca gtatttacga cgccatacaa gcaggttttg gcaaagttgt atttattata
     4321 agagaagaat ttgtagatat gtttaaagaa aattttggga aaaaattaga aggcaaaata
     4381 aaagtagatt acgtttatca acaattagat agttatactt ttggaagaac tataccagaa
     4441 gaaagaaaaa aaccatgggg caccgcacat gccatacttt gttgcaaaga aaaagtaaac
     4501 gaaccatttg ctgtaattaa tgcagatgac tattatggta aaaatgcttt tgttaaagca
     4561 caccaatttt taactacagt tgttgccgaa aacaattatg caattatagg ctatgaactt
     4621 aaaaaaacat taagtgaaaa tggaagtgtg agtagaggtg tttgtgatgt agatgaggaa
     4681 ggaaatttaa ttgaaataaa cgagagaact aaaatttaca aaaatgaaaa aggagtaatt
     4741 acttatgaag acgaaactgg tttacatgaa gtaagtgaag ctgcaagtgt aagtatgaat
     4801 ttttttggat ttcatcctaa ttttattcta acctgcgaaa aatattttag tgaattttta
     4861 gataatgaaa taaataaccc taaaagcgaa ttttttattc ctttagtggt agataaattg
     4921 gtaaaaagca atacagcaaa agtatctgta ataccaacaa cagcacaatg gtttggtgtt
     4981 acctataaag aagatgcacc aggtgtacaa gcaagtatta ataatttaat tcagcaaggg
     5041 cagtacccta ataatctttg ggcttagtgg tttattatgt ataaaggcat tgctttttta
     5101 tattttgtat gctttacttt gtatattatt tacagtaggg taccagatta ttttgatgga
     5161 gaaactgcac ctgcaactat ccattggcta aaagacagta ctacaaacac aactatacct
     5221 aaagcagttt ttattcatgg taaaacaaaa tatgaggtag atgcccgtta tgttttaaga
     5281 agcctaccag aaaataaaaa actaactgtt atttatcttg ttaaaaagcc
//
LOCUS       DAOQHP010000124         5292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8839_length_5292_cov_3.691044, whole genome shotgun sequence.
ACCESSION   DAOQHP010000124 DAOQHP010000000
VERSION     DAOQHP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8839_length_5292_cov_3.691044"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLY81_05240"
     CDS             <1..721
                     /locus_tag="PLY81_05240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF29663.1"
                     /translation="YVIPFIEKKFTIKEEMRVLEIGCGEGGVLKAFLQKGCTGLGVEF
                     DIPRIEDAKQFMPQETQSGKIKFISKDIYNVNAATELDGLFDIIILKDVIEHIHDQAK
                     LIAWMKNFLQPHGIIFFGFPPWYMPFGGHQQMCRTKLMQMPYIHLLPKQLYKWLLKKN
                     NEAVDDLMEIRETQITIERFEKICKQTNYTIIHKTHYLLNPIYKWKFNVKPRKQFAFI
                     QAIPFVRNFFTTCVYYAITPN"
     gene            833..1453
                     /locus_tag="PLY81_05245"
     CDS             833..1453
                     /locus_tag="PLY81_05245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNF29664.1"
                     /translation="MNHLSKWRASTTVKGYNDWYQKKWDYSRRYILYEEIYKTENITT
                     QAIDYFEFGVCEGHSFKWWLQKNQNTTSNFYGFDTFEGLPEKYGFFEKGTMAATIENL
                     NIKDTRASFYKELFQDTLNPFLENYKSSNKKIIHLDADIFSSTIFTLSQLFRFLNKGD
                     IIIFDEFAVPTHEFMAFKIFSESFYLNYEVIGATNNYLFLAIKITK"
     gene            1475..2290
                     /locus_tag="PLY81_05250"
     CDS             1475..2290
                     /locus_tag="PLY81_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HNF29665.1"
                     /translation="MIHIVFEQANVNALKNAIELDENLQGDVLEIKDDYAVGPLYNIY
                     ESEGYQQRREWWKNLLENSPYKEQLDIVDDKLTVHNLIKKLEENTEEKVWIWAGQNQH
                     DVCGYYWLMSQLKNFQSRIFILYLNNLPFINEKGQIFYPQHLSEIQPKEFLKAKKLAR
                     EITLSEFELDPDEWKKLSNENAMVRILEGGKKIVSKEENFYDNEILSAINNEPQKLNK
                     ILLHILNKMKIKTGDVFLVWRINELAKQMKIEISGDWNKGWKEMEMSLLKTPQ"
     gene            2321..4822
                     /locus_tag="PLY81_05255"
     CDS             2321..4822
                     /locus_tag="PLY81_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNF29666.1"
                     /translation="MMSDTNNNESGVQLQFKNWFLDYASYVILERAVPAIEDGLKPVQ
                     RRILHAMKEMDDGRFNKVANIIGQAMQYHPHGDASIGDALVNMGQKDLLIETQGNWGD
                     IRTGDDAAAPRYIEARLSKLALEIAFNNKTTNWQLSYDGRKNEPIVLPMKFPLLLAQG
                     AEGIAVGLSTKILPHNFIEIIEASIKYLKGKKFELYPDFQTGGLIDVSNYNEGRRGGK
                     IKVRCIIEEIDKKSLVIKDVPFGVTVAQLCESITKANDSGKIKIKKLTEFTGKNVEIQ
                     IDLAAGISSDITIDALYAFTDCEVSISPNACIIENNKPKFVGVQELLKISVDETKMLL
                     QKELQIKLAELEDKWHYTSLEKIFFEEKIYKELEQKYASWELVLQAIENAFQPFIKKL
                     KRKSISREDVLKLTEKPVRRIYKLDIDDLIEQIKAIDAEIVQVNNHLEHLTDFTITYF
                     ENLQKKFAKGRERKSEIKLFDTIKVQHITIANTKLYVNREEGFIGTSLKKDELLFECS
                     DLDDIIVFTKRGIMKVVKVGDKVFIGKDIIHAAIFKKNDERTTYNMIYVDAKSGISYA
                     KRFNVTGITRDKEYDLTKGSDKSKVHYFSSNANGEAEMVKIILSPNCTARNKELEFYF
                     EELEIKGRSSQGNIVTKYAVKSIKFKEAGRSTLSGIKLWFDDIYGRLNTEEKGQYLGM
                     FEDDKLLLIYKDGYYEITDTELTQRLDGDKILLIEKFNPQKIITAIYLDNDKQQFNVK
                     RFYIETTTLKSKFLFIKEGDGNYLETITTATEPILQVQTGRGQQVRKAKFKIDKMVEV
                     MGWKAIGAKLMDFSKTVEMEWIQVPKVENNQTELF"
     gene            complement(4891..>5292)
                     /locus_tag="PLY81_05260"
     CDS             complement(4891..>5292)
                     /locus_tag="PLY81_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF29667.1"
                     /translation="FMLKSGNVNVENGRITKGQFVIDMANLKVTDKAGVKLEGHLKAD
                     DFFDVTKHPEAIFEIENVNYTSETAVELTGTLTIKGIKVPIKFPGIVRGVSDKKLFAQ
                     AFFSLDSRLLPITDKYAASDVQIMVYLYANK"
BASE COUNT         2113 a          671 c          892 g         1616 t
ORIGIN      
        1 atatgttatt ccttttatag aaaaaaaatt tacgataaaa gaagaaatga gggttttaga
       61 aattggctgt ggcgaagggg gcgttttaaa agccttttta caaaagggat gtacaggatt
      121 aggggtagag tttgatatac ccagaatcga agatgcaaaa caatttatgc cgcaagaaac
      181 acaatctggt aaaattaaat ttatatcaaa agatatttat aatgtgaatg cagctaccga
      241 attagatggc ttatttgata ttatcatttt aaaagatgtt atagaacata tacatgatca
      301 agcaaagcta attgcatgga tgaaaaattt tttacaaccg catggtatta tcttttttgg
      361 ctttcctcct tggtatatgc cttttggtgg ccatcagcaa atgtgcagaa caaaacttat
      421 gcaaatgcca tacattcatc tgctgccaaa acaactctat aaatggttac ttaaaaaaaa
      481 taatgaagct gtagatgatt taatggaaat tagagaaacc caaataacta ttgaacgttt
      541 tgaaaaaata tgcaagcaaa caaactatac aataattcat aaaacacatt atctcttaaa
      601 ccctatttat aaatggaagt ttaatgtaaa accaagaaaa caatttgcat ttatacaggc
      661 aattccattt gtaagaaatt tttttacaac ctgtgtatat tatgcaataa ctccaaatta
      721 attttcaatg acacaattag atattataag aaaaaccaaa tcactgttta tgaaatggaa
      781 gctacacaac ttgtttagcc cattcaatgg ttttttactg aacctctatt acatgaatca
      841 tttaagcaaa tggagagcat ctacaactgt aaaaggatac aacgattggt atcaaaaaaa
      901 atgggattat agtagaagat atatactata cgaagaaatt tataaaaccg aaaacattac
      961 tacacaagca atagattatt ttgaatttgg cgtgtgcgaa ggacattctt ttaaatggtg
     1021 gctgcaaaaa aaccaaaaca ctacaagtaa tttctatggt tttgatactt ttgaaggcct
     1081 gcctgaaaaa tatggatttt ttgaaaaagg aacaatggct gcaaccattg aaaacttaaa
     1141 tattaaagac acaagagcaa gtttttataa agaacttttt caagataccc ttaacccttt
     1201 tttagaaaat tataaaagta gtaataagaa aattattcat ttagatgctg atatttttag
     1261 ctctaccatt tttaccttat ctcagttatt tagattttta aataaaggag atataataat
     1321 atttgatgaa tttgctgtgc caacgcatga gttcatggct tttaaaattt tttctgaaag
     1381 cttttatctt aactacgaag taataggggc aaccaataat tatttgtttc ttgctataaa
     1441 aattactaaa taaaatttaa actatttata ctacatgata catatcgttt ttgaacaagc
     1501 aaatgtgaat gccttaaaaa atgcaattga actagatgaa aatttacaag gtgatgtgtt
     1561 agaaataaaa gatgattatg ctgttgggcc attgtataat atttatgaat cagaaggtta
     1621 tcagcaaaga agagaatggt ggaagaattt attagaaaac tcaccttaca aagagcaatt
     1681 agatattgtt gatgataaat taacagttca caaccttata aaaaaattag aagaaaatac
     1741 agaagaaaaa gtttggattt gggcaggtca aaaccaacac gatgtgtgtg gctattattg
     1801 gttaatgagt caattaaaaa attttcaaag cagaatattt attttatact taaataattt
     1861 gccatttata aatgagaaag gtcaaatatt ttatccgcag catttatcag aaattcaacc
     1921 caaagaattt ttaaaagcaa aaaaattagc tcgtgaaatt actttaagcg aatttgagct
     1981 tgaccctgat gaatggaaaa aacttagcaa tgaaaatgca atggttagaa ttttagaagg
     2041 aggtaaaaaa attgtaagta aagaagaaaa tttttatgac aatgaaattt tatctgcaat
     2101 aaataacgaa ccacaaaagc tcaataaaat tttactccat attttaaata aaatgaaaat
     2161 aaaaacggga gacgtttttt tagtttggag aataaatgag ttggcaaagc aaatgaaaat
     2221 agaaattagt ggagattgga ataaaggatg gaaagaaatg gaaatgagtt tgcttaaaac
     2281 tccacaataa attacatgat tgataaattt gaattttaaa atgatgtccg atacaaacaa
     2341 caatgaatct ggtgtacaac tgcaatttaa aaattggttt ttagattatg catcttatgt
     2401 aattctagaa cgtgctgtgc ctgctattga agatggattg aaacctgtac aacgcagaat
     2461 tcttcatgca atgaaagaga tggatgatgg gcgttttaat aaagtagcca acattattgg
     2521 gcaggcaatg caataccatc ctcatggaga tgcaagcatt ggtgatgcat tggtaaatat
     2581 gggccagaaa gatttattaa tagaaactca gggaaattgg ggagatataa gaacaggaga
     2641 tgatgctgct gcaccacgtt atattgaagc aagacttagc aaacttgctt tagaaattgc
     2701 ctttaataat aaaaccacta attggcaatt aagttacgat ggcagaaaaa atgagccaat
     2761 tgtattgcct atgaaatttc cattactgct tgctcaaggt gcagaaggca ttgcagtagg
     2821 gttaagtaca aaaattttac cacacaattt tatcgaaatt attgaagcct ctatcaaata
     2881 tttaaaaggt aagaagtttg aactttatcc agattttcaa actggtggtt taatagatgt
     2941 aagtaattat aatgaaggta gaagaggtgg caaaataaaa gtgagatgta ttattgaaga
     3001 aattgataaa aaaagcttag taattaaaga tgtgccattt ggtgttacgg tagcacagct
     3061 ttgtgaaagt attacaaaag caaacgattc cggaaaaatt aaaataaaaa aacttactga
     3121 gtttacaggc aaaaacgttg aaattcaaat tgatttagcc gctggcatat caagcgatat
     3181 tactatagat gccttatatg cttttactga ttgcgaagta agcattagcc ccaatgcatg
     3241 cattattgaa aataacaaac caaaatttgt tggtgtgcag gaattattaa aaatttctgt
     3301 agatgaaaca aaaatgttat tacaaaaaga gctgcaaatt aaattagcag agcttgaaga
     3361 taaatggcat tatacttctt tagaaaaaat attctttgaa gaaaaaatat ataaagaatt
     3421 agaacaaaaa tatgcctcgt gggagcttgt tttacaagca attgaaaatg cctttcaacc
     3481 atttattaaa aagttaaaaa gaaaaagtat tagtagagaa gatgtgttga aacttactga
     3541 aaaacctgta agaagaattt ataaattaga tatagatgat ttaatagaac agataaaagc
     3601 tattgatgct gaaattgtac aggtaaataa ccatttagag catcttacag attttacaat
     3661 tacctatttt gaaaaccttc agaaaaaatt tgcaaaaggt agagaaagaa aatcggaaat
     3721 taaattgttt gacacaatta aagtgcaaca tattaccatt gccaacacaa aattatatgt
     3781 gaatagggaa gagggcttta taggaacaag tttaaagaaa gatgaattat tatttgaatg
     3841 ctccgactta gatgatatta ttgtttttac caaacgtggc ataatgaaag tggtaaaagt
     3901 aggagataaa gtttttattg gaaaagacat tattcatgcc gcaattttta aaaagaacga
     3961 tgaacgtact acctacaaca tgatttatgt agatgcaaaa tcgggtatta gttatgcaaa
     4021 aagatttaat gtaacaggta ttaccagaga taaagaatat gacttaacca aaggcagcga
     4081 taaaagtaaa gtacattact tttcatcaaa tgcaaatggt gaagcagaaa tggtaaaaat
     4141 aattttaagc cctaattgta cagctagaaa taaagaatta gaattttatt ttgaagaatt
     4201 agaaattaaa ggccgaagca gccagggaaa tattgtaact aaatatgcag taaaatctat
     4261 caagtttaaa gaagctggta gaagtacatt aagtggaatt aaactatggt ttgatgatat
     4321 ttatggtagg ttaaatacag aagaaaaagg gcagtattta ggaatgtttg aagacgataa
     4381 attacttctt atttacaagg acggatatta tgaaattaca gatacggaat taacacaaag
     4441 attggatgga gataaaattt tactaatcga aaaatttaac cctcaaaaaa ttattactgc
     4501 tatttattta gataatgata agcaacaatt taatgttaaa agattttata tagaaactac
     4561 aactttaaaa tctaaatttt tatttattaa agaaggagat ggaaattatt tagaaacaat
     4621 taccacagca acagagccaa tattgcaggt gcaaacaggg cgtggacaac aagtaagaaa
     4681 agctaagttt aaaatagaca aaatggttga agtaatgggc tggaaggcaa taggtgcaaa
     4741 gcttatggac tttagtaaaa ctgttgagat ggagtggata caagtaccta aagtagaaaa
     4801 caatcaaaca gaattatttt aaataaaaag agccaagttt ttcaactttg gctctttatt
     4861 aataaaacca caaaatatac aatcatccaa ttatttattt gcgtataaat aaaccattat
     4921 ttgtacgtca gaagcagcat atttatctgt aataggtaat aatcttgaat ctaagctaaa
     4981 gaaagcttga gcaaataatt ttttatcaga aacaccacgt acaataccag gaaacttaat
     5041 aggcacttta atacccttta tggttaatgt acctgtaagt tctacagcag tttctgaagt
     5101 gtaatttaca ttttcaatct caaaaattgc ttccggatgt tttgtaacat caaaaaaatc
     5161 atcggctttt aaatgccctt ctaatttaac gccagcttta tctgtaactt ttaaattagc
     5221 catatcaatt acaaattgcc ctttggttat tcttccattt tcaacattta catttccgct
     5281 ctttagcata aa
//
LOCUS       DAOQHP010000125         5279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8874_length_5279_cov_3.861600, whole genome shotgun sequence.
ACCESSION   DAOQHP010000125 DAOQHP010000000
VERSION     DAOQHP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8874_length_5279_cov_3.861600"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..610
                     /gene="nusG"
                     /locus_tag="PLY81_05265"
     CDS             5..610
                     /gene="nusG"
                     /locus_tag="PLY81_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNF29668.1"
                     /translation="MMEEITVQETVQQQPAENTKWYVLRVVSGKERSVKEYLDKDIAR
                     NGWTTIVKQVFLPMEKVYKVQNGKKVMREKNYFPGYVMLEVIDGKLTDDLVQHISNIS
                     NIMHFLTDGKGSKGNIISLRKAEVNKMLGKVDEMNDLGVTISEPYIVGETIKIIEGPF
                     NDFNGVIEEVNDEKKKLKVVVKIFGRSTPVELNYVQVEKLN"
     gene            825..1307
                     /locus_tag="PLY81_05270"
     CDS             825..1307
                     /locus_tag="PLY81_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29669.1"
                     /translation="MKKLNLLGTMLLGLLVLSTISCKKSTTSGSGSGSLTGSWSLVNA
                     KVEMLSGSTVVTSGTVTRAQIDADPVTYADLQFFKSLTFTSSTVTNGTGSTINYTIKT
                     GTDGNKYFVETGSADTDPDDRLYFYFEGGNLRIYNTQPGSPVAGSPTTQKVTMYYNKV
                     "
     gene            complement(1376..2533)
                     /locus_tag="PLY81_05275"
     CDS             complement(1376..2533)
                     /locus_tag="PLY81_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF29670.1"
                     /translation="MSYLKFKANNIFSGTEMFSDEYVLIAKQDGTIEAIVKQSEAGDD
                     LQYFNGILSPGFINTHCHLELSHLKGIIREKTGLVNFILDIVANRSSKEDIILQAIKN
                     AEDTMLKNGIVAVGDICNTTYTLYQKKQNNLAYYNFIETSGWLPNIAEKKYNEAVNLY
                     NQFSTITKKCSIVPHAPYSVSNELWCLIEKHFQNKIISIHNQEANAENELFLNGTGAF
                     MYLYQQMNITNNSFQPTKKSSLQSYFHWLENTQNILLVHNTCTSEKDIHFLHQQPEFI
                     KHKLFFCLCPNANLYIEDTLPNINLLRESNIQLTLGTDSLASNNNLNIVNEIKTLQTH
                     YPKITLAEILQWATINGAKALDMEDTLGSFEKGKKPGIILLENTNDIKRLV"
     gene            complement(2564..>5279)
                     /locus_tag="PLY81_05280"
     CDS             complement(2564..>5279)
                     /locus_tag="PLY81_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29671.1"
                     /translation="YKKDNVTYFLYLARMYKNLNTASLKICKKKFKDVLAEFMESKQN
                     QYTEDIRKRKNATKGKRTISEDVSKHNYYNFQINPNPKNNAYAMVQFKKGMYSVILND
                     NYYYVKTLLKRGVRVLQGDQSPNYPIMAWDGKGTRLLVIYAEDAKVKLFVWDYVARYK
                     RFKQVLEGVDQVIDASFMLDANTVILSAVKNGHTDIFIYKIQENKLEQITNDVYDDLN
                     PTYVSFPNRSGIIFSSNRPSANAPMEDTAIPSRNRFNVFLVDILNKSATKQITQLSSL
                     QYSNASYPMQYNVNHFTFVADETGIGNRYAGFFSTQRDGLDTLYYIGEDIIRNPTKNE
                     LDSSLAAWRIEKPDSVSYFQVYKDSTYTFPITNYQSSILETRIAGNNGMVSEVRREGD
                     YKYLFKLQVDDKALKNRNINPALSNYMKQVRGIVKQQKGKPNKSKKETTTVKADKPTT
                     DTTKPTNKKVFQTEFDDSVENTDTANTTNKTTPANTGNKESEKIELKKRLFNYQLKFS
                     TDYILGGISNNILINRYQMYGGGNGPVQLNNGNDVNWSFRVGISDVMEDIKIVGGLRF
                     GQSLSDKDAFISFQNFRKRLDWGVTYYRSVVSNFFPAGGYGNRLNTNLYQLNVSYPFD
                     ETKSLRATIGFRSDRGIMKAEDHISLPFTDTITKYALARMEYVYDNTLNPAQNIWKGL
                     RWKTYMDFNLPMVKGASKNFNFNFGFDARNYLEIYKNIIWATRAAGDFSWGEQKIIYY
                     LGGVDGWIGPKFNNMNRPAPDQTYAFQSLAVNMRGFNQNIANGNNAIVINSEIRVPVF
                     ATFLNKPINNAFLRNFQLVQFIDLGTAWNGKFNGIQRPYTIFPGSNSDDPVSVRIKAG
                     GIGPFVGGYGFGARSTLLGYFLKADIAWEMKGIFKGKPIFYFAMGLDF"
BASE COUNT         1755 a          820 c          773 g         1931 t
ORIGIN      
        1 attaatgatg gaagaaataa ctgtacagga aaccgtacaa caacaaccag cagaaaatac
       61 taaatggtat gtgttgagag ttgttagtgg aaaagaacgt agcgtaaaag aatacttgga
      121 taaagatata gctcgtaatg ggtggactac aattgtgaaa caggtttttt taccaatgga
      181 aaaagtatat aaagtacaaa atggtaaaaa agtaatgagg gaaaaaaact attttccagg
      241 ttatgtaatg cttgaagtaa ttgatggaaa gctgactgat gatttagtac agcatataag
      301 taatatttct aacatcatgc actttttaac tgatggtaaa ggatctaaag gaaacattat
      361 ttctttacgt aaagcagagg ttaataaaat gcttggtaag gttgatgaaa tgaatgattt
      421 aggtgtaact atcagcgaac catatattgt tggcgaaaca attaaaatta ttgaaggtcc
      481 attcaacgat tttaatggtg ttattgaaga agtgaatgat gaaaagaaaa aattaaaagt
      541 agtagttaaa atatttggaa gaagcacacc agtggagtta aattatgttc aagtagaaaa
      601 attgaactaa aaataataca attaaatttt acgaaaggag cttatggctc ctttttttat
      661 tgtctgaaca ttctttttat ttagttactt ctaaaaaaag gctttatgtt ataattaaaa
      721 ttagaaggta ttgatagtaa agatttaagt taattaaaag ataaactcat gcatgaatat
      781 aaaaaaataa gtatatttgt tttttatcac ataaaaaatt aaatatgaaa aaattaaatt
      841 tattaggtac aatgctactt ggcttattag ttttaagtac tatttcttgc aaaaaaagta
      901 ctacaagtgg tagtggttct ggctcattaa caggttcatg gagtttagta aatgcaaaag
      961 tagaaatgtt atcaggttct acagtggtaa cttcaggtac agttactaga gcacagatag
     1021 atgctgaccc tgtaacttat gctgatttac aatttttcaa atcacttact tttacaagta
     1081 gcactgttac taatggtact ggttcaacta ttaattatac aataaaaaca ggtacagatg
     1141 ggaataaata tttcgtagaa acaggaagtg cagatacaga cccagatgat cgtttgtatt
     1201 tttattttga aggaggtaat cttcgtatct ataacacaca accaggatca ccagttgcag
     1261 gaagcccaac aactcaaaaa gtaaccatgt actataacaa agtgtaattt attttattca
     1321 ttatacttta aaaaacgatt gccttaaatt aaaaggcaat cgttttttgt ttaaactaca
     1381 ctaatctttt tatatcatta gtattttcta ataaaataat acctggtttt ttgccttttt
     1441 caaaacttcc caatgtatct tccatatcta aagcctttgc tccattaatg gtagcccatt
     1501 gtagtatttc tgctaatgta attttgggat aatgtgtttg taaagttttt atttcattaa
     1561 ctatatttaa attgttatta ctagctaaac tatctgtacc taaagtaagt tgtatgttac
     1621 tttctcttaa taaattaata ttgggtaaag tatcttctat atataaattg gcattaggac
     1681 acaaacaaaa gaagagttta tgttttataa actctggttg ttgatgcaaa aaatgaatgt
     1741 ctttttcaga agtgcatgta ttgtgtacca ataaaatatt ctgagtgttt tccagccaat
     1801 gaaagtaaga ttgcaaacta cttttttttg tgggttgaaa tgaattattt gtaatattca
     1861 tttgttgata caaatacata aaagcccctg tgccattaag gaataactca ttctctgcat
     1921 ttgcctcttg attgtgaatt gaaattattt tattttgaaa atgtttttct attaaacacc
     1981 ataattcatt ggatacagaa taaggagcat gcggaacaat agaacatttt tttgttattg
     2041 tagaaaactg attgtacaaa ttaacagcct cgttgtattt tttttctgct atgttaggca
     2101 accatccact tgtttcaata aagttatagt aggctaaatt attttgtttt ttttgataaa
     2161 gtgtataagt agtattacaa atatctccaa ctgcaacaat accatttttc agcatggtat
     2221 cttcagcatt ttttattgct tgtaaaatta tatcttcctt gctgcttcta tttgctacaa
     2281 tgtctaatat aaaatttact aagcctgttt tttctctaat aatacctttt aagtggctta
     2341 actcaagatg gcaatgggta ttaataaatc cgggagataa tattccatta aaatattgta
     2401 aatcatctcc agcctctgat tgttttacaa tagcttcaat agttccatct tgtttggcta
     2461 ttaaaacata ttcatctgaa aacatttctg ttccagaaaa aatattgttt gccttaaact
     2521 ttaagtagct caattttaaa ttaattaatc caataattgg gtattaaaaa tctaacccca
     2581 ttgcaaaata gaaaataggc tttcctttaa aaatcccttt catttcccat gcaatatcag
     2641 cttttaaaaa ataacccagt agtgtacttc ttgctccaaa accataacca ccaacaaatg
     2701 gaccaatgcc accagcttta attcttacag aaactggatc atcggaattg ctaccaggga
     2761 aaatggtata aggcctttga atgccattaa atttgccatt ccatgcagtg cctaaatcta
     2821 taaattgtac taactgaaag ttgcgtaaaa aagcattatt aataggtttg ttcaaaaatg
     2881 ttgcaaaaac aggcactcta atttcactat taattacaat tgcattatta ccattagcaa
     2941 tattttgatt aaagccacgc atgttaactg ctaagctttg aaaagcatag gtttggtctg
     3001 gtgcaggcct gttcatatta ttaaacttag gtccaatcca tccatctaca ccacctaaat
     3061 aataaattat cttttgttcg ccccagctaa aatcacctgc agctctggta gcccaaataa
     3121 tatttttata aatttctaga tagtttctgg catcaaaacc aaaattgaaa ttaaaatttt
     3181 tactggctcc ttttaccatt ggcaggttaa aatccatata tgttttccat cttaaacctt
     3241 tccaaatatt ttgagcaggg tttaaagtat tatcatacac atactccatt cttgccagag
     3301 catattttgt aatggtatca gtaaaaggta aagaaatgtg atcttcagct ttcattattc
     3361 ctctgtctga tctaaagcca atagttgctc ttaaactctt ggtttcatca aaaggataag
     3421 agacatttag ttgatacaag tttgtattta acctgttgcc atagccacca gcaggaaaga
     3481 aattagaaac cacacttctg taataagtta ctccccaatc taatcttttt ctaaaatttt
     3541 gaaatgaaat aaatgcatct ttatcgctaa gagattgtcc aaatcttaat cctcccacaa
     3601 ttttaatatc ttccattaca tcgcttatac caactctaaa actccagttt acatcgttac
     3661 cattgtttaa ttgaactggt ccattacctc caccatacat ctgataacgg ttaattaata
     3721 tattattact aatgcctcct aaaatataat ctgtactaaa ctttaattga taattaaaca
     3781 atcttttctt aagctcaatt ttttccgatt ctttattacc tgtatttgca ggagttgttt
     3841 tattggtagt atttgcagtg tctgtgtttt caacactatc atcaaactct gtttgaaaaa
     3901 cttttttatt ggttggtttg gttgtatcag tagttggttt atctgccttt acagttgttg
     3961 tttctttttt agatttattt ggtttgcctt tttgttgttt tacaattcct cttacttgct
     4021 tcatatagtt agataaagca gggttaatat ttctattttt taatgcttta tcatctactt
     4081 gcagtttaaa taaatactta taatctcctt ctcttcttac ttcactaacc attccattat
     4141 tgcctgcaat tcttgtttct aaaatagagc tttgataatt ggttatagga aaagtataag
     4201 tagaatcttt ataaacttga aagtagctta cactatcagg cttttctatt ctccaagcag
     4261 ctaatgagct atctaattca tttttggttg gatttctaat gatgtcctct ccaatatagt
     4321 aaagtgtatc taagccatct ctttgtgtag agaagaagcc tgcataacga ttgccaattc
     4381 ctgtttcatc ggcaacaaac gtaaaatggt ttacattata ctgcattggg taagatgcat
     4441 tgctgtattg taaactgctt aattgagtta tttgtttagt tgcactttta ttaagtatat
     4501 ccactaaaaa aacgttaaat ctatttctac ttggtattgc tgtatcttcc ataggggcat
     4561 ttgcacttgg cctgttggag gagaaaataa ttcctgaacg attaggaaaa gaaacataag
     4621 ttgggtttaa atcatcatac acatcgttgg taatttgctc tagtttattt tcttgaattt
     4681 tataaataaa aatatcggta tgcccatttt ttacagcact taaaataaca gtattagcat
     4741 ctaacataaa cgaggcatca ataacctgat caacaccttc taaaacttgt ttaaatcttt
     4801 tgtagcgtgc cacataatcc caaacaaata attttacttt agcatcttca gcataaatta
     4861 ctaaaagcct ggttcctttg ccatcccaag ccattatggg ataatttggg ctttggtctc
     4921 cttgtaaaac cctaacacct ctttttaata atgtttttac ataataataa ttatcattta
     4981 aaataacact atacatacct tttttaaact gtaccattgc ataggcatta tttttagggt
     5041 tggggtttat ttgaaaatta taataattat gcttactaac atcttctgat atggttcgtt
     5101 tacctttagt tgcatttttt ctttttctga tgtcttctgt atattgattt tgcttgctct
     5161 ccataaactc tgcaagcaca tctttaaatt ttttcttgca tatttttaag gaggctgtgt
     5221 ttaagttttt atacattcta gccaaataca aaaaataggt tacattatct tttttgtat
//
LOCUS       DAOQHP010000126         5278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8880_length_5278_cov_3.533601, whole genome shotgun sequence.
ACCESSION   DAOQHP010000126 DAOQHP010000000
VERSION     DAOQHP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8880_length_5278_cov_3.533601"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLY81_05285"
     CDS             <1..360
                     /locus_tag="PLY81_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29672.1"
                     /translation="DNEENIRGRRVYYRLKQIDNDGSFQFSEVFSIHIPLNSKYTIYP
                     NPAMNFIIIEKTGINNNLSHQIQLTDLAGKILISKYYANANSQIILPIQHLPNGTYIL
                     RFTAGNESFIQKIVKRN"
     gene            complement(418..1026)
                     /locus_tag="PLY81_05290"
     CDS             complement(418..1026)
                     /locus_tag="PLY81_05290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF29673.1"
                     /translation="MLLFIHPDNPNPRNIKTVVECLKDGGTIIYPTDTIYGLGCSIYE
                     HKAIEKVAKIKNVSPEKAHLSFICYDLSDLSKYTKSISTPLYRMLKNYLPGAYTFILP
                     ASKEVPKILHSKKKTIGLRVPDNNIARTIIKELGSPILSASLPGEMVEEYTDPELMYE
                     NFKHLVDIVIDGGAGGMIPSTVVDCSQEPYQLIRKGLAAWND"
     gene            complement(1032..2024)
                     /locus_tag="PLY81_05295"
     CDS             complement(1032..2024)
                     /locus_tag="PLY81_05295"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNF29674.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTEIIPVASKKSIGKEIE
                     FKGKKYKVVSMEDAIKAKPALALFSAGGSTSLEWANKFAEANITVIDNSSAWRMDESK
                     KLVVPEVNATVLTVNDRIIANPNCSTIQMVVALQPLHKKYKIKRVVVSTYQSVTGTGM
                     KAVDQLFNERNNKEGAMAYKYKIDLNAIPQIDVFLDNGYTKEEMKMVKETCKIMQDET
                     IKVTATTVRIPVIGGHSESVNVEFDNEFELEEVKEILTNSPGIIVQQNDAEQFYPMPI
                     WAHEKDEVFVGRIRRDESQPKTLNLWIVSDNLRKGAATNAIQIAEYLYKNGFLN"
     gene            2199..2768
                     /locus_tag="PLY81_05300"
     CDS             2199..2768
                     /locus_tag="PLY81_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNF29675.1"
                     /translation="MFIVFSSAIAQTNYKQYIKQYQQEYVAKHEVVVGDDKQFFSFFK
                     ADENFKTNSTVTLLSDTIGFIMKTSGTKDKKFYRYAIINFKLYKKPFRLTLYSNKIIK
                     DSSQTNAYMFLPFTDNTCGNESYSGGRYLDFELADIKQNNLSLDFNKAYNPYCVYALG
                     YNCPIPLRENHLQINIRAGEKMFKGKKKK"
     gene            complement(2780..3511)
                     /locus_tag="PLY81_05305"
     CDS             complement(2780..3511)
                     /locus_tag="PLY81_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF29676.1"
                     /translation="MSSTLNKAMLLAAGLGTRLQPFTNHHPKALAIVNNKTLLQRNIE
                     YLQQFGISNVIINVHHFASQITDALQQNNGWGSNITISNESNEVLETGGGLQKAAWYF
                     DNTEHFLLMNVDILTNLNLYELLNAHIQNKALATLAVTDRESSRYLLFNENNKLCGWR
                     NVKTGEEKIPVPTNPLFQKAFSGIQIIQTKLLSLIQQKGKFSMIDVYLSLAKENNIQC
                     FNHSHTKLIDVGKPENLERAASMFE"
     gene            complement(3521..4963)
                     /locus_tag="PLY81_05310"
     CDS             complement(3521..4963)
                     /locus_tag="PLY81_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNF29677.1"
                     /translation="MNIIVNNIESLFYNWQKSAPLKIEKLQQSGSDRIYFRVFGSHQN
                     YIATYNLNIQENKSFIAFARHFKNKGLNVPEIYCHNEDYTIYIQQDVGEKSLLNLLEE
                     YGQNDFVFNFFKESLKQLAAFQIIGNEGMDYSLCLTAQEFGKEAILSDLLYFKYYFLD
                     TLKLPYDKQAMLKDFDALSTYLSYSEHKYFMFRDFQSRNIMLENNKISFIDFQGGMKG
                     ALQYDVASLLWQAKAALSNEWKDALLEYYMDEAEKLLHQKIERNSFISQYHGYVLIRL
                     LQVLGAYGFRGLFERKAHFLTSIPLGLLNLKYFIENKKVGITTPEFDRVLKIIISNNI
                     FEKFTPQQANDETRLLVTINSFSYKNGIPADTSENGGGFVFDMRGILNPGRQEVYKYL
                     SGKDKPVQDFLDQQTKMPNFLNSVWDLIDINVENYLTRDFENLMINFGCTGGQHRSVY
                     AAEQTARHLRNKYKLKVILNHTNQANWLRK"
     gene            complement(5039..>5278)
                     /locus_tag="PLY81_05315"
     CDS             complement(5039..>5278)
                     /locus_tag="PLY81_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29678.1"
                     /translation="NLFKQQRPYIYFNADDKYGVIDSNWLLIVRNDKFKGLYKYRNNN
                     VTNYANEYKDIVNRMQTYAESNLQTFQYLLKNKKL"
BASE COUNT         1723 a          854 c          704 g         1997 t
ORIGIN      
        1 gacaatgaag aaaatataag aggcagaagg gtttattata gattgaagca aattgacaat
       61 gatggaagtt ttcaattcag cgaagtattt agcatacata ttcctttaaa cagtaaatac
      121 actatttatc ctaaccctgc aatgaatttt attattattg aaaaaacagg cattaataat
      181 aatttgtcgc atcaaattca acttaccgat ttggcaggta aaattttaat aagtaagtat
      241 tacgcaaatg ccaatagtca aattatcctt ccaattcagc atttaccaaa tggtacttat
      301 attttaaggt ttacagcagg caatgaaagt tttattcaaa aaattgtaaa aagaaactaa
      361 cagaaaattg gtagaataat tttaataaaa ctgtgttgtt aaaaatggaa tgaggagcta
      421 atcattccag gcagctaatc cttttctaat taattgataa ggttcttgtg agcaatccac
      481 aactgttgat ggaatcattc ctcctgcacc tccatctata acaatatcta ctaaatgttt
      541 aaagttttca tacattaatt cagggtcagt atattcttca accatttcgc caggtaatga
      601 tgctgaaaga attgggctac caagttcttt tattattgtc ctggcaatat tattatctgg
      661 cacacgcaag ccaatagtct tttttttact atgtaaaatt tttggcactt ctttacttgc
      721 aggtaatata aaagtgtaag caccgggcaa gtaatttttt agcatccgat ataatggtgt
      781 agaaattgat ttagtgtatt tgctcaaatc gctaaggtcg taacaaataa aacttaaatg
      841 agccttttca ggcgaaacgt tttttatttt ggcaactttt tctatggctt tatgttcata
      901 aatactacaa cccaaaccat aaattgtatc tgttggataa ataatagttc caccatcttt
      961 taaacactct acaactgttt ttatgtttct aggattggga ttatctgggt gaataaataa
     1021 tagcataaat attaattcaa aaatccattt ttatataaat attctgcaat ctgaatagca
     1081 ttagtagctg caccttttct aagattatca ctcactatcc aaagatttaa agttttgggt
     1141 tgagattcat ctcttctaat tctaccaaca aaaacctcat ctttttcgtg tgcccaaata
     1201 ggcattgggt aaaattgttc tgcatcattt tgttgaacaa ttataccggg tgaattggtt
     1261 aaaatttctt taacctcctc aagttcaaat tcattatcaa attctacatt cacactttca
     1321 ctatgtccac caataacagg aatacgaacg gttgtagctg taacttttat ggtttcatct
     1381 tgcattattt tacaagtttc tttaaccatt ttcatttctt ccttagtata accattatct
     1441 aaaaaaacat caatttgtgg tatagcattc aaatctattt tatacttata agccatagca
     1501 ccctctttat tatttctttc attaaaaagt tggtcaactg ctttcatacc tgtacctgta
     1561 acactttgat aagttgaaac aactactctt ttaattttat attttttatg tagtggctgt
     1621 aatgcaacta ccatttgtat tgtagagcaa ttagggttag caataattct atcattaaca
     1681 gttaacacag ttgcatttac ttcgggcaca actaattttt tactttcatc catacgccag
     1741 gcactactat tatcaataac tgtaatattg gcttctgcaa acttgtttgc ccactctaaa
     1801 cttgtgcttc ctccggcact aaaaagtgct aaagcaggtt ttgcttttat agcatcttcc
     1861 atacttacta ctttatactt tttgccttta aactcaattt cttttccaat acttttcttg
     1921 cttgctacag gtataatttc tgttacagga aaatttcttt ctgccagtac ctgtaacatt
     1981 tttgttccta ctaagccagt ggctcctact actgctactt tcattataat ttttgttcta
     2041 ataatattat ggaaaaaaat taattcatat aattttttat ggcaacaaaa gtaattagtg
     2101 ttttggttat tgtgtaaaaa ttattcggtt tgcttaattt tatatttatt atgattaaaa
     2161 aaatgaaaaa taaaaaactt ctacaactta ttttgtttgt gtttatagta ttttcttctg
     2221 caatagctca aactaactat aagcagtaca tcaaacaata ccaacaagag tatgtagcta
     2281 aacatgaagt tgttgttggt gatgataaac agtttttttc tttttttaaa gctgatgaaa
     2341 attttaaaac caattcaact gttacccttc ttagcgatac tattggtttt attatgaaaa
     2401 cttctggcac aaaagataaa aaattttatc gctatgctat aattaatttt aaactttata
     2461 aaaagccatt caggttaact ttatatagta ataaaataat taaagacagt agccaaacta
     2521 atgcatacat gtttttacct tttacagata atacttgtgg taatgaaagt tatagtggtg
     2581 gaaggtattt agattttgaa ttagccgata ttaaacaaaa caatttaagt ttagatttca
     2641 acaaagccta caacccttat tgtgtatatg ctttgggcta taattgccct atacctttaa
     2701 gagaaaatca tttgcaaatt aatattcgtg caggcgaaaa aatgtttaag ggtaaaaaga
     2761 aaaaatgata caataaattt tactcaaaca tgcttgctgc cctttccaaa ttctctggct
     2821 tgcctacatc tattaatttt gtatggctat gattaaaaca ttgaatatta ttttcttttg
     2881 ctaaacttaa ataaacatct atcatagaga acttaccttt ttgctgtatt aggcttaata
     2941 atttagtttg aattatttga ataccgctaa atgctttttg aaataatgga ttagttggta
     3001 ctggtatttt ctcttctcct gtttttacat ttctccaacc acataattta ttgttttcat
     3061 taaacaataa atatctcgat gattccctat ctgtaacagc taaagtagct aaggctttat
     3121 tttgaatgtg agcattcagt agttcatata aattcagatt agtaagtata tccacattca
     3181 tcagtaaaaa atgttccgtg ttatcaaaat accaagcagc tttttgcaag cctccaccag
     3241 tttctaatac ctcattgctt tcattgctaa ttgtaatgtt actaccccat ccattattct
     3301 gttgcaacgc atctgtaatt tggctggcaa aatggtgtac attaattatt acattactta
     3361 taccaaactg ttgtaaatat tcaatatttc tttgtagtaa agttttattg tttacaatag
     3421 ccaatgcttt gggatgatgg ttggtaaagg gttgtaacct tgtgcctaaa cctgctgcta
     3481 ataacattgc tttatttagt gtactactca ttgggtgtaa ttattttctt aaccaattag
     3541 cctggttggt atggtttaga attactttta gtttatactt atttcttaaa tgtcttgcag
     3601 tttgttctgc agcatacaca cttctgtgtt gcccaccagt gcaaccaaaa tttatcatca
     3661 ggttttcaaa atctctcgtt aaataatttt ctacatttat atctattaaa tcccaaacac
     3721 tatttaaaaa attgggcatt ttagtttgct ggtctaaaaa atcttgtaca ggtttgtctt
     3781 tgccagataa atatttatac acttcttgcc ttccggggtt taaaattcct ctcatatcaa
     3841 aaacaaagcc tcctccattt tctgaagtat cagcaggtat tccattttta taactaaaac
     3901 tattaatggt aacaagcagt cttgtttcat catttgcttg ttggggtgta aatttttcaa
     3961 aaatattatt gctgataatt atttttaaaa ctctgtcaaa ctcaggtgtg gtaatgccaa
     4021 cttttttatt ctcaataaaa tatttaagat tcaataatcc aagtggaatg ctagttaaaa
     4081 aatgtgcttt tctttcaaac aaacctctaa agccataagc acctaaaacc tgtaatagcc
     4141 ttattaaaac ataaccatgg tattggctaa taaatgaatt tctttcaatt ttttgatgta
     4201 ataatttttc tgcttcatcc atataatact ctaataaagc atctttccat tcattgctta
     4261 atgctgcttt tgcttgccat aaaagcgatg ctacatcata ttgtaaagca cctttcatac
     4321 caccttgaaa gtctataaac gatattttat tattttcaag cattatattt ctgctttgaa
     4381 aatctctaaa cataaaatac ttatgttccg aataggacaa gtaggtgctc aatgcatcaa
     4441 aatcttttag cattgcttgt ttatcgtaag gtagctttag agtatctaaa aaataatact
     4501 taaaatacaa caaatcgcta agtatagctt ctttaccaaa ctcttgtgca gtaaggcaaa
     4561 ggctataatc catgccttca ttgcctataa tttgaaaagc tgctaactgt tttaaacttt
     4621 ctttaaaaaa attaaataca aaatcatttt gtccgtactc ttctaataaa tttaataacg
     4681 acttttcgcc aacatcttgc tgaatataga tggtataatc ttcattatgg caataaattt
     4741 ctggtacatt taaaccttta tttttaaaat gccttgcaaa ggcaataaac gatttatttt
     4801 cttgaatatt caagttataa gtagctatat aattttgatg gctaccaaaa actctaaaat
     4861 aaattctatc acttccgctt tgttgtaatt tttcaatttt tagaggtgca gatttttgcc
     4921 aattgtaaaa gagggattca atattattta ctataatatt cataacaaat ttttctgtaa
     4981 cgaatgtagt gtcttttata ggaagcagtg ctgctagttt aattttttga ataaatattt
     5041 ataacttttt attctttaat aaatattgaa aggtttgtaa gttagattct gcataggttt
     5101 gcattctgtt tacaatgtct ttatactcgt tggcataatt ggtaacatta ttatttctat
     5161 acttataaag tcctttaaat ttatcgttac gtacaattaa taaccaatta gagtctatta
     5221 ctccatactt atcatctgca ttaaagtaaa tatatggtct ttgttgttta aataaatt
//
LOCUS       DAOQHP010000127         5277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8883_length_5277_cov_3.622367, whole genome shotgun sequence.
ACCESSION   DAOQHP010000127 DAOQHP010000000
VERSION     DAOQHP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_5277_cov_3.622367"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..729
                     /locus_tag="PLY81_05320"
     CDS             100..729
                     /locus_tag="PLY81_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNF29679.1"
                     /translation="MRALVLVLVISIFSLNVGYTQNDVEAKKVLDAVSTKVKSFSAIT
                     GNFVIKTITSKGKLNGTKSGTISMKGSKYVLKQGKTQIICDGNNTYSYDGSKTITVAA
                     ADDADKTLTPQKIIAGSYSKDFTYKLVSSKGNFNEIELKPIDKRKNFQKVNVFVDKVK
                     SMITKAKVLDKSNNTLEFSFTNLNTNAKLADKIFVFNKANYPKDVEVLD"
     gene            739..1146
                     /locus_tag="PLY81_05325"
     CDS             739..1146
                     /locus_tag="PLY81_05325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF29680.1"
                     /translation="MFESTTQLRVRYAETDQMNVVYYGNYAQYFEVGRAESIRNLGVT
                     YKQLEETGVIMPVVEMHVKYLRSAHYDDLLTIKTMIKELPTGHKVEFHQEVYNEANKL
                     LTIGKVVLYFIDGATKQKTAIPTIFKDKLEKFF"
     gene            1203..2726
                     /locus_tag="PLY81_05330"
     CDS             1203..2726
                     /locus_tag="PLY81_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNF29681.1"
                     /translation="MSPIVLFSFVIGYFLLLLAVAWYTSKNANNDSFFIGNKNSNWML
                     VAFGMVGTSLSGVTFVSVPGTVGSAGFQYFQVVIGYCIGYLAVAYILLPLYYKMNLTS
                     IYNYLEHRFGNYSYKTGALFFILSRTLGATARLYLVINILQLFILDSMGVSFWVTTLI
                     ILALIVLYTFEGGVKTIVYTDTLQTTCMLLGLLVCIVAIMSKMDLSLSNSIHALSDKG
                     MLQIFNTDIHSSGFFLKQIIGGAFITITMTGLDQEMMQKNISVRTLKDSQKNVLTLSV
                     ILFFVNLLFLFMGGLLYLYAGQNGITSKGDDLFPFIALNSHFSVAVAVIFIIGLISAL
                     FPSADGALTALTSSFCIDILGLKKKTQFTEQQKKRTRLTVHFTFTIIFFLCVLAFKWI
                     NDKSIIDVILKVAGYTYGPLLGLFAFGIFSKRKINDKLSVVVCLIAPVLVIGIDIINN
                     SEWFIHKLNITGSIANSLQNLSQQIFNGFRIGIELLIINGLFTFIGLYIISKPAPKS"
     gene            complement(2727..3263)
                     /gene="dcd"
                     /locus_tag="PLY81_05335"
     CDS             complement(2727..3263)
                     /gene="dcd"
                     /locus_tag="PLY81_05335"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNF29682.1"
                     /translation="MILTDKRILEEMDKGTIKIVPYNRADLGSNSYDVHLGSTLATYI
                     DKELDAKKHNTIEYFEIPDEGYVLQPHAFYLGVTLEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVRIYKGMPIGQLIYFPVEGEIEIKYNEKRNAK
                     YSGQPNKPIESMMWKNKF"
     gene            3335..3970
                     /locus_tag="PLY81_05340"
     CDS             3335..3970
                     /locus_tag="PLY81_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNF29683.1"
                     /translation="MPLFYQQNINQFTRLAIWKIEESESFFSNTVPLQNSITHNHKRL
                     QHLAGRFLLKYLYPSFPYHAIEIADTKKPFLPNEDFHFSISHCGDYAAAIVSTKNRVG
                     LDIELSTDKINRIASKFLNETEFKNNAINLQEITVAQQNKLTLLWSAKEAIFKWWGNG
                     KVDFSEMIKTNSFVFSENGTIPIQFKNHLLQAHYKQLNNMYLVYVISDINT"
     gene            complement(3967..>5277)
                     /locus_tag="PLY81_05345"
     CDS             complement(3967..>5277)
                     /locus_tag="PLY81_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF29684.1"
                     /translation="VSLAFYIAYTVGSIIYLFISKAMGIDLLNKLGYKNGIALGLTIS
                     ALGTLLFYPAANTGSFNLMLTGLFIVALGFSLQQIAANPLTVVMGNPKTGSQRLSLAG
                     GINNFGTTIGPILVSLAIFGSVTSNNTEASIESVKIPYLTLGVVFLIVAAFFKFSSIP
                     NKIDLDHVAEEEADSNDKIMHKKSAFQYPQLWLGMIGIFVYVGVEVATASNLPEYLKV
                     KMGIGTEKVAPYISLYWASLMIGRWTGSLEAFNFKKGTKDILKFLMPYIAFGVFLLVN
                     KIAQHDITPFYVYAFVIIAMIIGDILSKGNPARMLLIFSAMGIIALLIGMFTHGMVSV
                     FAFISVGLFCSTLWPCIFTLAVAGLGKHTNEGSSYLIMMIMGGGFISYLQGYVAADHL
                     LGIQYSYIVCVACFVYLAFFAIAVKRILQKQGIDYDTNTADIKH"
BASE COUNT         1858 a          780 c          793 g         1846 t
ORIGIN      
        1 ttttaactaa aaacctatgt tttgttaaac ctttataagt ttaaggcatc aaaaaatgtg
       61 taggcactat atttgccacc ataaaattta aaatttaaga tgagagcatt agtactagtt
      121 ttagttattt ctattttttc tttaaatgtt ggatatactc aaaatgatgt agaagcaaaa
      181 aaagtgttag atgcagtgag tacaaaagta aaatctttta gtgctattac aggaaatttt
      241 gtaattaaaa caataactag taaaggaaaa ttgaatggca caaagtctgg caccatttca
      301 atgaaaggca gtaaatatgt tttaaaacaa ggaaagacac aaattatttg cgatggtaat
      361 aatacttata gctacgatgg cagcaaaacc attactgtag cagctgctga tgatgcagac
      421 aaaactttaa ctccacaaaa aattattgct ggtagttaca gcaaagattt tacttataaa
      481 ctagtttcta gtaaaggcaa ttttaatgaa attgaattga agccgattga taaaagaaag
      541 aattttcaga aagtaaatgt ttttgtagat aaagtaaaat caatgattac taaagcaaaa
      601 gtgttagata aaagcaataa tactcttgaa tttagtttta ccaaccttaa tactaatgca
      661 aaacttgctg ataaaatttt tgtttttaac aaagccaatt atcctaaaga tgttgaagtt
      721 ttagattaat tttttttcat gtttgaaagt actacacagc ttagagtaag atatgccgaa
      781 actgaccaaa tgaatgtggt ttattatggt aattatgctc aatactttga agttggaaga
      841 gcagaaagta taagaaattt aggagttacc tataaacaat tagaagaaac aggagtaata
      901 atgccagtag tggaaatgca tgtaaaatat ctacgttcgg cacattatga tgatttgttg
      961 accataaaaa ctatgattaa agaattacca acagggcata aagttgagtt tcatcaagag
     1021 gtatataatg aggctaataa actattaact attggtaaag tagttttata ttttatagat
     1081 ggtgccacca aacaaaaaac agcaattcct actattttta aagataaact agaaaagttt
     1141 ttttagttgt tgaaaatacc taaagtttgt gtattgttta ttaatcaaac ataacactat
     1201 acatgagccc aattgtttta ttttcttttg taataggtta ctttttatta ttactagcag
     1261 tagcatggta cactagtaaa aatgccaata acgattcgtt ttttatagga aataaaaaca
     1321 gtaattggat gcttgttgcc tttggaatgg taggcacaag tttaagtggc gttacttttg
     1381 taagtgtgcc tggaacagtt ggtagtgcag ggtttcagta ttttcaagta gttattggat
     1441 attgtattgg atacttggca gttgcctata ttttattgcc cttatattat aaaatgaacc
     1501 ttacttctat ttacaattat ttagaacata ggtttggtaa ttacagctat aaaacagggg
     1561 ctttattttt tattttatcc agaacattag gtgcaactgc aaggctatat ttagtaataa
     1621 atattttaca gttgtttatt ctagactcta tgggtgtttc tttttgggtt actactttaa
     1681 ttattttagc actcattgta ctatacactt ttgaaggtgg tgtaaaaaca attgtttata
     1741 ccgatacctt acaaacaacc tgtatgctat taggcttatt agtttgtatt gttgccataa
     1801 tgagcaaaat ggatttaagc ctaagcaata gcatacatgc tttgagtgat aaaggcatgc
     1861 tccaaatttt taatacagat atacattcct ctggattttt tcttaaacaa attattggag
     1921 gagcctttat tacaattact atgacagggc ttgatcagga aatgatgcaa aaaaatattt
     1981 ctgtaagaac attaaaagac tctcaaaaaa atgttttaac gcttagtgtt attttatttt
     2041 ttgtaaatct tttgttcttg tttatgggag gtttattgta tttatatgca gggcaaaatg
     2101 gtataacttc taaaggcgat gacttatttc cgtttatagc cttaaatagt catttttctg
     2161 ttgcggttgc agtaattttt attataggtt taatttctgc tctttttcct agtgccgatg
     2221 gagctttgac tgctctcact tcttcatttt gtatagatat acttggttta aaaaagaaga
     2281 ctcagtttac tgaacaacag aaaaaaagaa ctaggttaac cgtgcatttt acttttacta
     2341 ttattttctt tttatgtgta ttggccttta aatggattaa cgataaatct attatagatg
     2401 taattttaaa agtagctggt tatacgtatg ggcctttact tggcttgttt gcatttggaa
     2461 tattttctaa aagaaaaata aacgataagc tttctgttgt tgtttgttta atagcaccag
     2521 tattggtaat tggtattgat attataaata attcagaatg gtttattcat aaattgaata
     2581 ttactggaag cattgcaaat agtttacaaa acttatcgca acaaattttt aatggtttta
     2641 gaattggaat agaattatta attataaatg gcttatttac ttttatagga ttgtatataa
     2701 tttctaaacc agcacccaaa agttaattaa aatttattct tccacatcat actttcaatg
     2761 ggcttgttag gctgcccact atattttgca tttctctttt cgttatattt tatttcaatt
     2821 tcgccttcta ccggaaaata aattaactgt ccaataggca ttcccttata aattcttaca
     2881 ggttgtttta ccgatatttc taaagtccag ttgccacaaa aaccaacatc gcctttgcct
     2941 gcagtagcat gtatatctat tcctaaacga ccagtgctgc ttttgccttc taaaaaaggt
     3001 acatgggcat gggtttctgt atattctaaa gttaccccta aataaaatgc atgaggttgt
     3061 aaaacatatc cttcatctgg tatttcaaaa tactcaattg tattgtgttt ttttgcatct
     3121 aattctttat ctatataggt tgctaaagtg ctgcctaaat gtacatcata actgttgcta
     3181 cctaaatcgg ctctgttata tggaacaatt tttatagttc ctttatccat ttcttctaaa
     3241 attcttttat ctgttagtat catattgtaa taataaactt tatttgcaaa taaaattaat
     3301 cttcaagcac aaacattaaa aaacaaattt gtatatgcct ttgttttatc aacaaaatat
     3361 taaccaattc acaaggcttg caatttggaa gatagaagaa tcagaatctt tttttagtaa
     3421 tactgttcct ttgcaaaact ctataacaca taaccataaa aggttacagc atttagcagg
     3481 tagattttta ttaaagtatt tataccctag ttttccttat catgcaattg aaattgcgga
     3541 tacaaaaaaa ccatttttgc caaatgaaga ttttcatttc tctatttctc attgtggaga
     3601 ttatgctgca gcaattgtta gtacaaaaaa tagggtaggt ttagatattg aattaagtac
     3661 tgataaaatt aatagaattg cctctaagtt tttaaatgaa acagaattta aaaacaatgc
     3721 aattaaccta caagaaatta ccgttgcaca acaaaataaa ttaacacttt tatggagtgc
     3781 taaagaagca atttttaaat ggtggggtaa tggcaaagtt gattttagtg aaatgataaa
     3841 aaccaattcc tttgtttttt ctgaaaatgg cacaatacca attcagttta aaaaccattt
     3901 attacaagca cattacaagc agttaaataa tatgtacctg gtgtacgtta tttctgatat
     3961 aaatacttaa tgttttatat ctgcagtatt agtatcataa tctattcctt gcttttgtaa
     4021 aattcttttt acagcaattg caaaaaatgc aagataaaca aagcaagcaa cgcatacaat
     4081 atagctgtat tgaataccta ataaatgatc ggcagctaca taaccttgta aatagcttat
     4141 gaaaccacca cccataatca tcataattaa atagctactt ccttcgtttg tgtgtttgcc
     4201 taaacctgct actgctaatg taaaaataca aggccacaaa gtactacaaa ataagcctac
     4261 acttataaat gcaaatacac taaccatacc atgtgtaaac atgccaatta gtaaggcaat
     4321 aatgcccatt gcagaaaaaa taagtaacat tctggcagga ttgcctttac ttaaaatgtc
     4381 gccaattatc atggctataa taacaaatgc atatacataa aaaggagtta tatcatgttg
     4441 ggcaattttg tttaccaata aaaaaactcc aaatgctata tagggcatca aaaattttaa
     4501 aatatctttt gtaccttttt taaagttaaa tgcttctaaa cttcctgtcc atctaccaat
     4561 cattaaactt gcccaataca aagaaatata aggggctact ttttcggttc caatacccat
     4621 tttaactttt aaatattctg gtaaattact tgcagtagcc acttctacac caacataaac
     4681 aaaaattcct atcataccta accagagttg agggtattga aatgcacttt tcttgtgcat
     4741 tattttatcg ttactatctg cttcttcttc tgctacatga tctaaatcta ttttatttgg
     4801 aatagatgaa aatttgaaaa atgcagcaac aattaaaaac actacaccca acgtaagata
     4861 aggtattttt acactttcaa tactagcttc tgtgttgtta gaagttacac ttccaaaaat
     4921 ggctaagctt actaaaatag gaccaatagt agtaccaaaa ttattaatac cacctgctaa
     4981 acttaacctt tgagagcccg ttttaggatt gcccataaca acagttaaag ggtttgctgc
     5041 aatttgttgt aaagagaagc ctagagcaac aataaacaac cctgtaagca ttaaattaaa
     5101 ggagcctgta ttagctgcag gataaaaaag tagagtgccc aatgccgaga tagttaaccc
     5161 tagagcaata ccatttttat atcctaattt atttaaaaga tctattccca ttgctttaga
     5221 aataaacaaa taaataatgg aaccaactgt ataagcaata taaaaagcta aagaaac
//
LOCUS       DAOQHP010000128         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8889_length_5274_cov_4.249090, whole genome shotgun sequence.
ACCESSION   DAOQHP010000128 DAOQHP010000000
VERSION     DAOQHP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8889_length_5274_cov_4.249090"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLY81_05350"
     CDS             <1..581
                     /locus_tag="PLY81_05350"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNF29685.1"
                     /translation="VVGWEYLHKGGREWKVKVTRTEASQGREFTDISTLLDLRKANKN
                     EWKQIVFHRYGMMEQGDTMEIIAAENPTEIHGIFVQKFEGKHTWIYKKQTDTEVVVHI
                     TKKEKTGLGDDGYAVVNEYDLRPYPPAKRHDMFYEAFAEIKPGEAFEFYNDHDPLPLY
                     YQMEAESKDPFKWEYIIKGPEEWKVRVIKIKP"
     gene            complement(639..1145)
                     /locus_tag="PLY81_05355"
     CDS             complement(639..1145)
                     /locus_tag="PLY81_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29686.1"
                     /translation="MNKPLHDFFTKDHHRLEGYLVNVIEADNEINVEYYHLFRKGLLR
                     HIKMEEKILFPAAAKVNKELVQKIIPQFRLEHGALTALLVPPPTKAIINAIQFVLNKH
                     DFEEEKDGGLYDICEALTIAQTNTLLQQLIATEEVPLHPPNPAPIALESAIRALQRAG
                     YVYDEICK"
     gene            1292..1675
                     /locus_tag="PLY81_05360"
     CDS             1292..1675
                     /locus_tag="PLY81_05360"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNF29687.1"
                     /translation="MAKPIIYSCSGCSSAAQMANYMAVKLDRKDVAEMSCIVGVGGNV
                     KKLVKTAKSGRKIIVIDGCPLNCSKACLNNQGIEPDLHFELTKFGVKKQNHIDFDMEQ
                     ANNLLELIENKIDEAIVKETSLVLN"
     gene            1678..2031
                     /locus_tag="PLY81_05365"
     CDS             1678..2031
                     /locus_tag="PLY81_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29688.1"
                     /translation="MKNKIELLAPVGSFESLHAAIQAGANAIYFGVEQLNMRAKSINS
                     FMLDDLAAIQALCIPKNISTYITLNTVMYEHDMQLLKAILKEVKKHKITAVIASDFSV
                     MEYCKQLQIPLHIST"
     gene            2047..2913
                     /locus_tag="PLY81_05370"
     CDS             2047..2913
                     /locus_tag="PLY81_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNF29689.1"
                     /translation="MESVKFFSTFSDAVVLARELTLKQVKQITDEIKRKKIKGVSGQL
                     MRIEIFIHGALCMAISGKCYLSLHSQNASANRGACTQNCRHAYKVTDIETNEELVIDN
                     EYIMSPKDLCTIDIIDQIIESGVSILKIEGRGKGPEYVYTVTKTYQEAITAVEEKEYT
                     KEKIDKWKTELEKVYNRGFWEGYYLGRKLGEWTLNPGSMATEKKVYIGKGKKYFSKIS
                     VGEFVVETGKVEIGDTIIITSPNFGIVKENIEQLTVNGNNATVAVKGDSITLRCKNKI
                     SAKDKIYKLVQS"
     gene            2914..3138
                     /locus_tag="PLY81_05375"
     CDS             2914..3138
                     /locus_tag="PLY81_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNF29690.1"
                     /translation="MPNIIHYRSKCIGCNICYEMMPSTWRMSKKDGKATLLKAEQKRQ
                     TSVLQIDTNLIEEAKKVANACPVKIIKVNG"
     gene            complement(3135..3572)
                     /locus_tag="PLY81_05380"
     CDS             complement(3135..3572)
                     /locus_tag="PLY81_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF29691.1"
                     /translation="MFSKTTEYALRATIYIAQNSSVVNKIGIDEIAKAIDSPKSFTAK
                     VLQLLTKDSRVISSVSGPKGGFYMTDEAKNLPVRSVLEATGESIVLEKCVLGLNKCSE
                     KKPCPMHSRYKDIKEQLVNLFTNEPISKLAEETKLGNVFIGNC"
     gene            3811..5025
                     /locus_tag="PLY81_05385"
     CDS             3811..5025
                     /locus_tag="PLY81_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNF29692.1"
                     /translation="MSHNILPIIKIGGVNHHNKEWIAIYCKKDFTINSIIKKIKGNTF
                     SASKQCWLIPYEKYNYDYLVKHLKELASIDDKLISIFKKEKEKKLTGITNKLSNQTLL
                     QISIQLPNQQKIHPVNKQVLEQVQQHLQLKAYSTSTRKTYLNEIAAFLATIKHVKASA
                     LTPNRIKDYLQYCYKELKLSENTMHSRINALKFYYEQVLKKDKFFYEIPRAKKPYKLP
                     NVLSEKEIVRLFNTLQNQKHKAILFIAYSAGMRVSEVVGLKISNIDSQRMQIKIEGAK
                     GKKDRYVNLSTIVLEVLRSYIKQCVPRPKKYLFESEIAGEPYSSRSAQKVFQRAKEKA
                     GISKNVTFHSLRHSFATHLLEKGVDIRYIQDILGHNNIKTTERYTHVKKEQIIQITSP
                     LDDLWLKGDIKI"
BASE COUNT         2105 a          739 c          845 g         1585 t
ORIGIN      
        1 atgtggtagg ctgggaatat ttacacaaag gcggcagaga atggaaagta aaagtaaccc
       61 gaacagaagc ttctcaaggc agagaattta cagatatttc tacattatta gatttaagaa
      121 aagcaaataa gaatgagtgg aagcaaattg tatttcatcg ttatggtatg atggaacaag
      181 gcgatacaat ggaaataatt gcagcagaaa accctacaga aattcatgga atttttgttc
      241 aaaaatttga gggtaaacat acttggattt ataaaaaaca aaccgataca gaagtagtag
      301 ttcatattac taaaaaagaa aaaacaggtt taggagatga tggctacgca gtagtaaatg
      361 aatacgattt aaggccttat ccgccagcaa aaagacacga tatgttttat gaagcttttg
      421 cagaaattaa acccggcgaa gcttttgagt tttataacga ccacgaccct cttccacttt
      481 attatcaaat ggaagcagaa agcaaagacc cattcaaatg ggaatatatt attaaagggc
      541 cagaggaatg gaaagttaga gtaataaaaa ttaaacctta attacttact aacaatagtt
      601 gaagaatagt ttgataaaat aattttatca aactattttt atttacaaat ttcatcataa
      661 acatagcctg ccctttgtaa ggctctaatt gccgattcta gtgcaattgg tgcaggattt
      721 ggtggatgaa gaggcacttc ttcggtagca ataagttgtt gtaataaggt atttgtttgt
      781 gctatggtta aagcttcaca aatatcatat aaaccaccat ctttctcttc ttcaaaatca
      841 tgcttattca acacaaattg aattgcatta ataatagctt ttgttggtgg tggtactaat
      901 aaagctgtta atgccccatg ttccaatcta aattgtggaa ttattttttg aaccaattct
      961 ttatttactt tggctgcagc aggaaaaagt attttctctt ccattttaat atgcctaagc
     1021 aaaccttttc taaataaatg gtaatactct acattaatct cattgtctgc ttcaataaca
     1081 tttaccaaat atccttctaa cctatgatga tccttggtaa aaaaatcgtg taatggttta
     1141 ttcatataaa aaatatcaat aataaaaaca tgtgaaaatt aagccaacat taaataaaat
     1201 aaagcaatta tgaacctata aagatgttgt catcctttat atctataaat ttgcaacgct
     1261 aaaattattg attattaaat taatggcaaa aatggcaaaa ccaattatat attcttgctc
     1321 tggttgctct agtgctgcac aaatggccaa ctacatggca gtaaaattag acagaaaaga
     1381 tgtagctgaa atgtcttgca ttgtaggtgt tggaggtaat gtaaaaaaat tagtaaagac
     1441 agctaaatct gggcgaaaaa taattgtgat tgatgggtgt ccattaaact gctctaaagc
     1501 ttgtttaaac aatcagggta ttgaaccaga tttacatttt gagctaacaa aatttggtgt
     1561 aaaaaaacaa aaccatatag attttgatat ggaacaagca aataacttat tagaattaat
     1621 agaaaataaa attgatgaag caatagtaaa agaaacaagc ttagtgttga attaaaaatg
     1681 aaaaataaaa tagaattatt agcccctgta ggctcttttg aaagtttaca tgctgcaata
     1741 caggcaggtg caaatgctat atactttggt gtagagcaat taaatatgcg tgccaaatca
     1801 attaactctt ttatgcttga tgatttagca gccatacaag cattatgtat tccaaaaaac
     1861 attagtacct atattacttt aaatacagta atgtatgaac acgatatgca gttgttaaaa
     1921 gcaattttaa aagaagtaaa aaagcataaa ataacagcag taattgcatc agatttttcg
     1981 gttatggagt attgtaaaca actccaaatt ccattacaca tttcaacata agctaatgtt
     2041 agtaatattg aatcggtaaa attcttttct actttttctg atgcagtagt tttagctaga
     2101 gagcttacac ttaaacaggt aaaacaaata accgatgaaa taaaaaggaa gaaaattaaa
     2161 ggtgtttctg gccaattaat gcgtatcgaa atttttatac atggtgcatt gtgtatggca
     2221 atttcgggta aatgttattt aagtttacat agccaaaacg catcggccaa tcgtggtgct
     2281 tgcacacaaa attgcaggca tgcctataaa gttacagata tagaaactaa tgaagaatta
     2341 gtaattgata atgagtatat catgtctcca aaagaccttt gcacaataga tattatagac
     2401 cagataatag aaagtggtgt aagtatttta aaaatagaag gaaggggcaa aggtccagaa
     2461 tatgtatata cagttaccaa aacctatcaa gaagcaataa cagcagttga ggaaaaagaa
     2521 tatacaaaag aaaaaataga taaatggaaa acagaactag aaaaagttta taaccgtggc
     2581 ttttgggaag ggtattattt aggaagaaaa ttaggagaat ggacattaaa cccaggctca
     2641 atggctacag aaaaaaaagt atatatagga aaagggaaaa aatatttttc caaaattagt
     2701 gtaggcgaat ttgttgtaga aaccggaaaa gttgaaattg gagacaccat cattattaca
     2761 agcccaaatt ttggaattgt aaaagaaaat atagagcaac ttacagttaa tggcaataat
     2821 gcaactgttg ctgttaaagg agatagcata accttacgtt gcaaaaataa aatttcggct
     2881 aaagataaaa tttacaaatt agtacaaagt taaatgccca atattattca ttatagaagc
     2941 aaatgtattg gttgcaatat ttgttatgaa atgatgccat ccacttggcg aatgtctaaa
     3001 aaagatggta aagcaacatt actaaaagca gagcaaaaaa ggcaaaccag tgttttacaa
     3061 atagatacca acttgataga ggaagcaaaa aaagtagcaa acgcctgccc tgtaaaaatt
     3121 attaaagtaa acggttaaca attaccaata aaaacatttc ccaatttggt ttcttctgct
     3181 agtttactta ttggttcatt agtaaataaa tttactaatt gttctttaat atccttatat
     3241 ctactatgca ttgggcatgg ttttttttcg gaacatttgt ttaaacctaa tacacatttc
     3301 tctaaaacta tactttcgcc ggtagcttct aatactgaac gtacaggtaa attcttagct
     3361 tcatctgtca tataaaatcc accttttggg ccacttaccg aactaattac tctactatct
     3421 ttggtaagta attgtaaaac ttttgcagta aaagatttag gagaatctat tgcttttgca
     3481 atttcatcta tacctatttt atttactacc gagctatttt gggcaatata aattgttgcc
     3541 cttaatgcat actccgttgt tttagaaaac atataattag cctgttctaa agttttgaat
     3601 aattaaagta aaaatatatt tttttaaaac agactaacaa aataaaagca tttacccatt
     3661 catgccaata attataaaat atttttttga aaaagtattc agattatgta gtctttattt
     3721 atatttaaaa agtaatttat aaacttctta tttaaagcaa tttgtttcat ttatgtgtga
     3781 gtgagatagc ggagctgtaa aaacttgtaa atgtcccata atattttacc aatcattaaa
     3841 ataggtggtg tcaatcatca caataaagag tggattgcaa tttattgtaa aaaagatttt
     3901 accattaata gtattattaa aaaaataaaa ggaaacacat ttagtgcaag taagcaatgt
     3961 tggttaatac cttatgaaaa atataattac gattatttag taaagcattt aaaagagctt
     4021 gccagtatag atgataaatt aatatccata tttaaaaaag agaaagaaaa aaaattaact
     4081 ggtataacaa ataaattaag taaccaaaca ttattacaaa tatcaataca gttacccaat
     4141 cagcaaaaaa tacaccctgt taataagcaa gttttagaac aagtgcaaca gcatttgcaa
     4201 ttaaaagctt atagcacaag tacaagaaaa acatatttaa atgaaatagc agccttttta
     4261 gcaacaataa aacatgtcaa agcatctgcc cttacaccaa atagaattaa agattattta
     4321 cagtattgtt acaaagaatt aaagctttca gaaaatacca tgcatagtag aataaatgca
     4381 ttaaaatttt attacgagca ggtactaaaa aaagataaat ttttttacga aatacccagg
     4441 gcaaaaaaac cctacaagct accaaatgtt ttatccgaaa aagagattgt tagattattt
     4501 aacacactac aaaatcaaaa acataaagca atattattta tagcatatag tgctggcatg
     4561 agagtaagtg aagttgtagg gttgaaaatt agcaatatag atagtcaaag aatgcaaata
     4621 aaaatagaag gagccaaagg caaaaaagat aggtatgtaa acttaagtac tatagtttta
     4681 gaagttttaa gaagctatat aaagcaatgc gtacctcgtc caaaaaaata cttatttgaa
     4741 agtgaaatag caggcgaacc ctattcatca agatcggcac aaaaagtttt tcaaagagca
     4801 aaagaaaaag ctggtattag taaaaatgta acctttcatt cattaaggca cagctttgca
     4861 actcatttgt tggaaaaagg cgtagatata agatatatac aagatatttt agggcataat
     4921 aatataaaaa caaccgaaag atatacccat gtaaaaaagg aacaaataat acaaattaca
     4981 agtccattag atgatttatg gctaaaaggc gacatcaaaa tataacacca aaggcgacat
     5041 attttcttct taaaaaacaa actaacctgt acaaaatcag ttcttattta actttatcga
     5101 atataattag ttcgtatatt tgagagttag cggtcattgt aacagacgag acaaccatca
     5161 gataattatg aacgaaattg aaacatacat aaagcagttt cccaacaatg ttcaggaaat
     5221 tttgcaaaac attaggaagt taataattga caatgcacct aatgcggacg aact
//
LOCUS       DAOQHP010000129         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_8985_length_5234_cov_3.931068, whole genome shotgun sequence.
ACCESSION   DAOQHP010000129 DAOQHP010000000
VERSION     DAOQHP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8985_length_5234_cov_3.931068"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLY81_05390"
     CDS             complement(<1..257)
                     /locus_tag="PLY81_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29693.1"
                     /translation="MQISSQIFNPFPGLRSFEENEDLLFFGREKQVTELVKKLRQEKF
                     LAVIGSSGSGKSSLVKSGLIPALHSGFMSGAGSSWKICTFRP"
     gene            complement(438..1145)
                     /gene="rnc"
                     /locus_tag="PLY81_05395"
     CDS             complement(438..1145)
                     /gene="rnc"
                     /locus_tag="PLY81_05395"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNF29694.1"
                     /translation="MQFLKNIFNITSKTVFIKQVENVLGFKTHKSNLYITALSHRSIK
                     DTPDQNNERLEYLGDAILSSIVADYLFKRYPYKGEGFLTEMRSKMVNRNQLNDIALKM
                     GLKKITLYNKIDNSLRNSQIFGNTLEAVVGAVYLDKGYKKTQDWVLKQILIPHMFVDD
                     LENIDINLKNKLIGWANKNNKELAFEVAEEKIISGRRLFIINTVIDGEVLSQGSGYNK
                     KDASHTAAQLAIEKLGL"
     gene            complement(1152..2405)
                     /gene="fabF"
                     /locus_tag="PLY81_05400"
     CDS             complement(1152..2405)
                     /gene="fabF"
                     /locus_tag="PLY81_05400"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNF29695.1"
                     /translation="MTLKRVVVTGLGAITPLGNNVETYWQNLVNGVSGADMITLFNAE
                     KFRTKFACEVKNFDPTEFLDRKEARKIDRFTQFALVVSDQAMNDAGLNKENINPDRIG
                     VVFASGIGGLITFQHEVQEFAKGDGTPRFNPFFIPKMILDIAAGQISMRHNLRGPNFA
                     VVSACASSTNAIMDAFNLLRLGKADIILTGGSEAVISEAGVGGFNAMKAMSERNDEPK
                     TASRPYDKDRDGFVMGEGAGMLVLEELEHAKARGAKIYCEVMGCGATADAHHITAPHP
                     DGLGAKNVMLAALEDANMQPTEIDYINTHGTSTPVGDGAEIKAVTEVFGEHAFKLNIS
                     ATKSMTGHCLGAAGALEAIASIMAVVHDTVPPTINHFTDDPEIDTQLNFTFNKAQQRT
                     VNAALSNTFGFGGHNACVIVKKYKA"
     gene            complement(2593..2829)
                     /locus_tag="PLY81_05405"
     CDS             complement(2593..2829)
                     /locus_tag="PLY81_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF29696.1"
                     /translation="MSDIATRVKKIIVDKLGVDEAEVTTEASFTNDLGADSLDTVELI
                     MEFEKEFSISIPDEQAETITTVGQAVTYLEEHAK"
     gene            complement(2945..3724)
                     /locus_tag="PLY81_05410"
     CDS             complement(2945..3724)
                     /locus_tag="PLY81_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF29697.1"
                     /translation="MTYSTLLTQLENGILIITINRPDKLNALNKDVFNDLDTIFNEVY
                     SNNQIQSVIIIGCGNKAFVAGADIGEFGSLSVEEGKKLAKRGQDVFNKIENSPKPVIA
                     CVNGFALGGGCELSMACHFRIAAENAKFGQPEVNLGLIPGYGGTQRLAQLIGKGRAIE
                     LLITANMIDASTALQFGLVNYVVPSDALLNKAKEILQTVNSKAPLAVAACIKAANAVW
                     KETVNGFDEEIQLFGNCFGTNDMKEGTNAFLEKRKAVFTGN"
     gene            3810..5165
                     /gene="mnmE"
                     /locus_tag="PLY81_05415"
     CDS             3810..5165
                     /gene="mnmE"
                     /locus_tag="PLY81_05415"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNF29698.1"
                     /translation="MATFSDWKDTIVALATPQGVGAIGVIRVSGESAIDIVDKLFPSK
                     NLNQQTTHTLHVGLLKNNNEVLDEVVVALYKAPKSFTGENVVEISCHGSYFIQEKIIE
                     TLTSNGCRIAKAGEFTQRAFLNGKLDLTQAEAVADLIASNSEASRNTAINNMKGNFSE
                     ELKKLREELIKFSALIELELDFSQEDVEFADRTQFIQLINKLLQTTKQLAASFKLGNV
                     IKNGVQVAIIGKPNAGKSTLLNTLLNEERAIVSDIAGTTRDTIEEVLNIDGILFRLID
                     TAGIRKHTVDDIEAIGIGKSFEKAEKADIVLYIYDATYTEKLEEEKQWLKTLNKPYIL
                     IANKADKIDNKNSQESIYISAKEKVNIDLLKQKLIEKTVGGEINTEATIITNIRHYDA
                     LQKLNHYLQETLRGLEENISGDLLALDIRQCLHHLGEITGEITNEDQLDYIFSKFCIG
                     K"
BASE COUNT         1737 a          877 c          817 g         1803 t
ORIGIN      
        1 ggcctgaaag tacaaatttt ccaactagag cctgcaccag acataaagcc actatgcaat
       61 gcaggaatta atcctgattt tactaaggaa gattttccac tacccgatga acctataact
      121 gctaaaaatt tttcttgcct cagtttttta accaattctg ttacttgctt ttctcttcca
      181 aaaaacaata agtcttcatt ttcttcaaat gaacgaaggc ctggaaatgg gttaaaaatt
      241 tggctactta tttgcatctg tattggcaat taaaatatca tttattcaac aaaatgaata
      301 atttgtttta taacttttag atgtacaata ttaaaaaata aattttaatg ttgaataatt
      361 aatagttaat ggaaaaagtg ggagtgttat aaatttataa aacgaatggg taggtaaatt
      421 tttttttgga tgggtactta taaacctagt ttttctattg ctagttgagc tgctgtatgg
      481 cttgcatctt ttttattgta tccgcttcct tgagataaaa cttccccatc tataactgta
      541 ttaataataa aaagccttct gccgctaata attttttctt cggcaacttc aaatgccaac
      601 tctttattat ttttattagc ccagccaatt agtttgtttt ttaagttaat atcaatattt
      661 tctaagtcat caacaaacat atgtggtatt aatatttgtt taagcaccca gtcttgtgtt
      721 tttttatatc ctttatctaa atacacagca ccaacaactg cttctaatgt attaccaaaa
      781 atttgactat tgcgtaagct attatctatt ttattataca gggtaatttt ttttaagccc
      841 attttaaggg caatatcatt tagttgattt ctgttaacca ttttgctacg catttcggtt
      901 aaaaatcctt cgcctttata tggatatctt ttaaacaaat aatcggctac aatgctgctt
      961 aaaatggcat cgcctaaata ttctaatcgt tcattatttt gatctggagt atcttttatg
     1021 cttctatggc taagtgcagt tatgtataaa ttacttttgt gtgttttaaa tcctaataca
     1081 ttttctacct gctttataaa aacagtttta gaagttatat taaaaatgtt ttttaaaaac
     1141 tgcacaaatt tttatgcttt atattttttt acaattacac aagcattgtg cccaccaaag
     1201 ccaaatgtgt tgcttagtgc agcatttact gtacgttgtt gagctttatt aaaagtgaaa
     1261 tttaattgag tatctatttc aggatcgtct gtaaagtgat tgatagtagg aggaactgta
     1321 tcatgcacta cagccataat gcttgcaatg gcttctaatg cacctgctgc acctaaacaa
     1381 tgcccagtca tgctttttgt agcactaata tttagtttaa aagcgtgttc tccaaacact
     1441 tctgttacag ctttaatttc tgcaccatcg ccaacaggcg tacttgtgcc gtgtgtgtta
     1501 atataatcaa tttcagttgg ctgcatattg gcatcttcta aagcagccag cattacattt
     1561 tttgccccta agccatctgg gtgaggagca gtaatatgat gagcatcagc agttgctcca
     1621 caacccatta cctcgcaata aatttttgct ccacgagctt tagcatgttc taattcttcc
     1681 aataccagca tacctgcacc ttctcccatt acaaaaccat ctctatcttt atcgtagggc
     1741 cttgatgctg ttttgggttc atcgtttctt tcgctcatgg ctttcatggc attaaagcca
     1801 ccaacaccag cttcgctaat tacagcttcg ctgccaccag ttagtataat atctgctttg
     1861 cctaaacgca gtaagttaaa tgcatccatt attgcattgg tgctacttgc acaggcactt
     1921 actacagcaa agttggggcc tcttaaatta tgcctcatac ttatttggcc tgcagcaata
     1981 tctaaaatca tttttggtat gaaaaatggg ttgaaacgtg gcgttccatc gccttttgca
     2041 aattcttgta cttcgtgttg aaaggtgatt aatccaccaa ttccactagc aaatacaact
     2101 ccaattctat cggggttaat attttctttg tttaagcctg catcgttcat tgcttggtcg
     2161 ctaacaacta aggcaaattg cgtaaaccta tcaatttttc tggcttcttt tctatctaaa
     2221 aattcggtag ggtcaaagtt ttttacttcg caggcaaatt tggttctaaa tttttctgca
     2281 ttaaataaag taatcatatc ggcaccagaa acaccattta caagattttg ccagtaagtt
     2341 tctacattat tacctaatgg tgttatggcc ccaagacctg taaccactac cctttttaaa
     2401 gtcatataca ataagctttt agttttataa acattgctaa gcaattaaaa aaatgcataa
     2461 caggttaata ctacaaatat aatattaaag ctaagaaagt ttattaaata aataaatccg
     2521 ccaactctga taattatatc tatgaatggc ggatttatgt taattattcc aattaattat
     2581 ggagtaaatc ttttatttgg cgtgctcttc taagtaagta acagcttgcc caacggttgt
     2641 aattgtttct gcttgttcat caggaataga gatgctgaat tctttttcaa attccatgat
     2701 taattcaaca gtatccaaag aatctgcacc taagtcgtta gtaaaagaag cttctgtagt
     2761 tacttctgct tcatctacac ctaacttatc aacgatgatt ttctttactc ttgttgcaat
     2821 gtctgacatt ttaaaaagtt ttagttataa tggcaaaaat agagctttta gttaaaatgc
     2881 aaaattaata ataattttta ttaaaattag ggaagaaaat gtagtttttt actgccctaa
     2941 aaggctaatt accagtaaaa acggcttttc ttttttctaa aaatgcatta gtgccttctt
     3001 tcatatcatt tgtaccgaag caattgccaa aaagttgaat ttcttcatca aaaccgttga
     3061 ctgtttcctt ccacacagca ttggctgctt ttatgcaagc tgcaactgct aaaggagctt
     3121 tagaatttac tgtttgtaat atttcttttg ctttattcaa tagggcatct gaaggaacaa
     3181 cataatttac taaaccaaat tgtaatgctg ttgaagcatc aatcatatta gcagtaatta
     3241 ataattctat agccctacct ttacctatta gctgtgcaag cctttgagtt cctccataac
     3301 caggtattaa accaaggtta acttcgggct gcccaaattt tgcattttct gctgcaatac
     3361 gaaaatggca tgccatggat aattcacaac ctccacctaa agcaaagcca ttaacacagg
     3421 caataacagg ctttgggcta ttttcaattt tattaaaaac atcctgccct cttttagcta
     3481 atttttttcc ttcttctaca cttaaacttc caaactctcc aatatcggca cctgcaacaa
     3541 atgctttatt tccacaacct ataataatta cagattgaat ttggttattg ctatatactt
     3601 cattaaatat tgtatctaaa tcattaaaaa catctttatt cagtgcattt agtttatctg
     3661 gtcggttaat agttattata agaatgccgt tttctaattg tgttagtagt gttgaataag
     3721 tcataaaatg aatttgttta tgcccgcaat ttaattataa aatttgatgt ttgtttctta
     3781 tgctattttc gctatttaaa aatattttca tggctacatt ttcagattgg aaagatacaa
     3841 ttgttgcatt agcaacacca caaggagttg gagcaattgg cgtaattaga gtaagcggag
     3901 aaagtgcaat tgatattgta gataaattgt ttccatccaa aaatttaaac caacaaacta
     3961 cgcatacttt acatgttgga ttactcaaaa ataataacga ggttttagat gaggttgttg
     4021 tggcattata caaagcccca aaatctttta caggagaaaa tgtagtagaa attagttgcc
     4081 acggttctta ttttattcaa gaaaaaataa tagaaacttt aacatctaat ggttgtagaa
     4141 ttgcaaaagc aggcgaattt acacaaaggg catttttaaa tggaaaacta gatttaacgc
     4201 aggcagaagc tgtagcagac ttaatagcaa gtaactcgga agctagcaga aatactgcaa
     4261 taaataatat gaaaggtaat ttttcggagg agctaaaaaa acttagagag gagttaataa
     4321 aattttcggc actgatagag cttgaattag actttagcca agaagatgta gagtttgcag
     4381 atagaacaca atttatacaa ttaattaata aactactaca aacaactaag cagttggctg
     4441 cctcatttaa attgggtaat gtaataaaaa atggtgttca agttgcaata ataggtaaac
     4501 ccaatgcagg gaaaagtact ttattaaata ctttattaaa cgaagaaagg gcaattgtaa
     4561 gcgatattgc tggtactaca agagacacta ttgaagaggt tttaaatatt gatggaatat
     4621 tgtttagatt aattgacact gcaggtataa gaaaacatac agtagatgac attgaagcaa
     4681 ttggaattgg aaaaagtttt gaaaaagcag aaaaagcaga tattgtttta tacatttacg
     4741 atgcaaccta cacagaaaag ctagaagaag aaaaacaatg gttaaaaact ttaaataagc
     4801 cgtatatatt aattgccaac aaagcagata aaattgataa taaaaatagc caggagtcta
     4861 tttatatttc ggcaaaagaa aaagtaaata ttgatttgtt aaaacaaaaa ctgattgaaa
     4921 aaactgttgg tggagaaata aatacagagg ctaccattat aaccaatata aggcattatg
     4981 atgctttaca aaaactgaat cattatttac aagaaaccct aagagggtta gaagaaaata
     5041 tatcaggcga tttgttagct ttagatatta ggcaatgctt acaccactta ggtgaaatta
     5101 caggtgaaat taccaatgaa gaccaattag attatatttt tagtaagttt tgtatcggaa
     5161 agtaattatc tttcttttat ttgttaagtg cctgttttta atataaactt taaaccatga
     5221 actgcaatta gtaa
//
LOCUS       DAOQHP010000130         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9000_length_5228_cov_3.614344, whole genome shotgun sequence.
ACCESSION   DAOQHP010000130 DAOQHP010000000
VERSION     DAOQHP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9000_length_5228_cov_3.614344"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..753)
                     /locus_tag="PLY81_05420"
     CDS             complement(43..753)
                     /locus_tag="PLY81_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29699.1"
                     /translation="MDKKNILLVEDDFLNRRYTKKILIENGYNVLESKNAIETMALLK
                     THTPDLIILDIHLGETDKNGINLAQNIKELFNIPFLFLTAYSATDVVEKAIATLPQSY
                     ITKPFKNIDLITAVELAIRQSNNQEKRKPVVLIKEGGFNIELLVDEIDYIESDGNYLL
                     IYTQEKVYKSRFTIKQILETLPAATFVQTHRAYIVNKNRIEKYSTKNVIVNNKEIPVS
                     KNYIDDIKVFEKMRNLYT"
     gene            complement(761..2482)
                     /locus_tag="PLY81_05425"
     CDS             complement(761..2482)
                     /locus_tag="PLY81_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNF29700.1"
                     /translation="MALKYSFLLSLFFYCHSVVAQNYIENSEIKKIDSLILYNQFETA
                     QKITDSIYKGLQSQKNNKALLLEIKYKKALILDRQEKPPNEPLSILLDIIDEVHAAKL
                     HSLLYRIYLLIALCHEKAANLELTNKYLNEAYSIYEKYKLENLYSMYCIRRGSYYRFL
                     EKIDSVFYFANQAKENAVAYNNEKVLVDSYILLAFAANRNKNYTAALECFFQLKNYRV
                     KYKDTSALAMSYINIAQCYKNMKNFQKALIYSDSSFVFFKSKPTFIYSYQYSKIKYEI
                     FEALGNMDSAYYYLKQYQQEWSIVKNHEVDVKTNELEKQYQSNKKEAIINSKNQQLFF
                     IIILLFVITVGSILIVRQNRKISKQNKIINNQIDELSKIIEQKQMLLSELQHRVKNNL
                     QHVISILEIQKESVDFNNIDELIRGNQNRIHSMALLHKKLNISENVNEVDLPKYISEL
                     SELVKDSYDNHTKKIQLNVKCEIDKIALEKALPIGLIIVELVSNSMKYAFLSRSIGII
                     NILLSTNHDTTENTFYYTDNGEGFDFNAIVNKGLGIEIIKGLISQLDGKYETSNSKGF
                     EFMIYFK"
     gene            complement(2582..3850)
                     /locus_tag="PLY81_05430"
     CDS             complement(2582..3850)
                     /locus_tag="PLY81_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29701.1"
                     /translation="MSDFTHHINYTAADINNYLQGKMSNAERNAFEKAALADPFLADA
                     LEGFEIAETTKALQMLKETDNLISASALKQNKNYNLADIENYLQNNMGNAERNAFEKA
                     TLADPFLADALEGFENANLTEAKKYIGETATTILSKEVGKAKVVTMPIYKKQWLRIAA
                     GFIIMIGAGATIWLLNNNTSTKKENTLAAITKEEIKQTPNTISALPTEKENTSEKIVN
                     NHQQNASNKKEKVKIEDFNIINDNALISSPQAAIKTTESSAGIFSTEDALNKNKPKDI
                     SVKSMVGEPPAFPNNGAVNAQNSRNIEERNAIASNLAEASKSKKATAAVLKISDNEQN
                     NKGITPIGGWKKFDDYINTKRAALHFTNELVESVIVTFLIDKEGKPYQIQAQSSIPEK
                     SVRAIELIENGVNWQVKNTTNNKAVVIIKL"
     gene            complement(3843..4412)
                     /locus_tag="PLY81_05435"
     CDS             complement(3843..4412)
                     /locus_tag="PLY81_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF29702.1"
                     /translation="MALTVKHIDNTADDNSLMLLYQQSLDQKSLATLFLRYNDLVFGT
                     CMKYFKDEETSRDAVMDIYQELVQKLPNHKVDNFKSWLYVVTKNHCLMQLRKNKKTTT
                     VEFDGQFMQSEDFSHLDNVLEKEQKLNSLEGCIETLNEEQKKSIQLFYLENKCYNEIA
                     DITGIEWNKVRSLIQNGRRNLKICMDKNV"
     gene            4568..5176
                     /locus_tag="PLY81_05440"
     CDS             4568..5176
                     /locus_tag="PLY81_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29703.1"
                     /translation="MKSAIFLQIATPCNENWEKMTPMEKGKFCASCAMQVTDFTLMTD
                     KEILQYLSTTRGKVCGRIHADQSNRALINYENKKSKKIQWTIAGLMSLLFSINKVNAQ
                     QKTVKESPKSSWLAANRNEDMVTIKQSVTVLKDNAKNLNTPYLHQVFAIENSLVSKSA
                     SAFLINNNDTTIIKEVKDTSLSFFDKASFEQTNITLETLFQI"
BASE COUNT         1680 a          787 c          698 g         2063 t
ORIGIN      
        1 attaaaattg caaatgttat aatagaaaat tagcttggct ttttaagtat ataagtttct
       61 cattttttca aaaactttta tatcatcaat atagtttttc gaaacaggaa tttctttatt
      121 attcactatt acatttttag tactatattt ttcaattctg tttttattta caatgtacgc
      181 tctatgtgtt tgtacaaaag ttgcagcagg aagtgtttct aaaatttgtt taatggtgaa
      241 acggcttttg tacacttttt cttgagtata aattaaaaga taatttccat cagattctat
      301 gtaatctatt tcatcaacaa gtagttcaat attaaaccct ccttctttta ttaatacaac
      361 aggtttacgt ttttcctgat tgttagattg gcgaattgca agttctactg ctgttatcaa
      421 atctatattt ttaaatggct tggtgatata ggattgtggc aaagtagcaa ttgctttttc
      481 aacaacatca gtggcactgt aggctgtaag gaacaaaaaa ggaatattaa atagctcttt
      541 aatattttgt gcaaggttta tcccattctt atctgtttct ccaaggtgaa tatcaagaat
      601 aataaggtct ggtgtatgtg tttttaataa tgccattgtt tcaatagcat ttttactttc
      661 caatacatta tatccgtttt ctattagtat ttttttggta tatcttctat tcaaaaaatc
      721 atcctctact aataaaatat tttttttgtc catcttgcca ttatttaaaa taaatcataa
      781 actcaaaacc tttactgttt gatgtttcat attttccatc taattgacta attaaacctt
      841 ttattatttc aatgcctaag cctttattta ctattgcatt aaaatcgaaa ccttcgccgt
      901 tatcagtata ataaaatgta ttttctgttg tatcgtgatt ggttgatagc aaaatattaa
      961 taataccaat gcttcggctt aaaaaagcat atttcatact attgctaact aattccacaa
     1021 taattaaacc tattggtaat gctttctcta aagctatttt atcaatttca catttaacat
     1081 ttaattgaat tttctttgta tgattatcgt aggaatcttt taccaattcc gaaagctctg
     1141 aaatgtattt tggcaaatct acttcattca cattctctga tatattcaac tttttatgca
     1201 acaatgccat agagtgaata cggttttgat tgcctctaat gagttcatca atattattaa
     1261 aatctacaga ttccttttgt atttccagaa tgctaataac atgttgtaaa ttatttttta
     1321 ccctatgttg caactcagag agaagcattt gcttttgttc tataatttta gaaagttcgt
     1381 ctatttgatt attgattatt ttattttgtt tactgatttt tctattttgt ctaacaatta
     1441 aaatagaacc aactgtaatt acaaaaagca ggatgatgat gaaaaaaagt tgttgatttt
     1501 tgctgtttat aatggcttct ttcttattgc tttggtattg tttttcaagc tcatttgttt
     1561 taacatctac ttcatgattt tttactatac tccattcctg ttgatattgt tttagataat
     1621 aataagccga atccatatta cctaatgctt caaaaatttc atactttatt tttgaatatt
     1681 gataactata aatgaaggtg ggcttgcttt taaaaaaaac aaaagaggaa tcactataga
     1741 tcaaagcttt ttgaaaattt ttcatgtttt tatagcattg agcaatgttg atgtatgaca
     1801 ttgctaatgc agacgtgtct ttatatttta ccctataatt ttttaactga aaaaaacatt
     1861 ccaatgcagc cgtataattt ttatttctgt tagcagcgaa tgcaagtaat atataagaat
     1921 caaccaaaac tttttcatta ttgtatgcta ctgcattttc ttttgcttga ttggcgaaat
     1981 aaaaaactga atcaattttt tctaaaaatc tataataaga acctcgtcta atacaataca
     2041 tgctgtacaa gttttcaagc ttatactttt cgtatatact atacgcttca ttaagatatt
     2101 tattggttag ctctaagttg gctgcttttt cgtggcacaa agcaattaac aaataaatac
     2161 ggtataagag tgaatgaagt tttgcagcat gtacttcatc tataatatcc aataatatgc
     2221 ttagaggttc gttagggggt ttttcttgtc tgtctaatat aagagccttt ttgtatttta
     2281 tctctaataa taaggcttta ttattttttt gcgattgtag tcctttgtaa atactatctg
     2341 ttattttttg agcagtttca aattgattat ataaaataag gctgtctatc tttttgattt
     2401 cactattttc aatataattc tgtgccacaa cactatggca gtaaaaaaat aaacttaata
     2461 aaaatgaata cttcaatgcc ataataaata gcaaatatca aaaataacac ttctgcaatt
     2521 tatataacaa tagagggata gaagtaagtt tttaggggca gaaagaatgt tgagaataat
     2581 tttacaactt aattataaca acagccttat tattcgtagt attttttact tgccaattaa
     2641 cgccattttc aattaattca atagctctta cagatttttc tggaatagag ctttgagctt
     2701 gaatttgata aggcttgcct tctttatcaa ttaaaaaagt aacaattaca gattctacta
     2761 gttcgtttgt aaaatgtaat gctgctcttt ttgtattaat ataatcatca aattttttcc
     2821 aaccaccaat aggagtaatt cctttattgt tctgttcatt atctgatatt tttagaacag
     2881 cagcagttgc tttcttactt tttgatgctt cagctaaatt agaagcaata gcattccttt
     2941 cttctatatt tctagaattt tgtgcattta cagcaccatt atttggaaat gcaggaggct
     3001 ctcctaccat gcttttaaca gaaatatctt tgggtttatt tttatttaaa gcatcttcag
     3061 tactaaaaat tcctgcactt gattcggtag tttttattgc agcttgtggc gaactaatta
     3121 atgcattatc attaattata ttaaagtctt caatttttac cttctctttt ttattggatg
     3181 cattttgctg atgattattt actatttttt cagaagtatt ttctttttct gtaggtaatg
     3241 cagaaatagt atttggagtt tgttttattt cttctttggt aattgctgct aaagtatttt
     3301 cttttttagt acttgtatta ttatttaaaa gccatattgt agctcctgca ccaatcatta
     3361 ttataaaacc tgcggcaatt cttaaccatt gctttttata tattggcata gttaccactt
     3421 ttgcttttcc aacttcttta cttaatatag ttgttgctgt ttcgccaata tattttttag
     3481 cttcggttag gtttgcattt tcaaaacctt ctaaagcatc tgctaaaaag ggatctgcta
     3541 atgttgcttt ttcaaatgca ttcctttctg cattacccat attgttttgt aaataatttt
     3601 caatatcggc taaattgtaa tttttatttt gtttaagtgc agaagctgaa atgaggttat
     3661 ctgtttcttt caacatttga agtgctttag tagtttcagc aatttcaaaa ccttctaaag
     3721 catctgctaa aaagggatct gctaatgctg ctttttcaaa agcatttctt tcagcattgc
     3781 tcattttgcc ttgcaaatag ttattaatat ctgctgctgt ataattaata tggtgtgtaa
     3841 aatcagacat ttttatccat gcaaattttc aggttacgcc ttccgttttg tattaaacta
     3901 cgtactttat tccattctat tccagtaata tctgctattt cgttatagca tttattttct
     3961 aaataaaaca actgaatact tttcttttgt tcttcattta aagtttcaat acaaccttcc
     4021 aggctattta atttttgttc tttttctaaa acattatcaa gatgcgaaaa gtcttcggat
     4081 tgcataaatt gtccatcaaa ttctactgtt gttgtttttt tattctttct taactgcatt
     4141 aaacagtggt tttttgttac cacatataac caacttttaa aattatccac cttatgatta
     4201 ggaagctttt gaactaattc ctggtaaata tccattacag catcacggct tgtttcttca
     4261 tctttaaaat atttcataca agtaccaaac actaaatcat tatacctgag aaataaagta
     4321 gccaacgatt tttgatctaa gctttgctgg tacaaaagca ttaaagaatt atcgtctgct
     4381 gtattatcaa tatgttttac agttaatgcc ataaggcact aagataaata tttaatgata
     4441 tatacaaata aactgttata aaaattgaaa taattctaaa gaaataaaca atttacccat
     4501 tgtagttcta tttattaaaa aagtatgtaa ttgttcaaaa ggcaacatct gtaactaaac
     4561 aaattatatg aaatcagcta tttttttgca gattgctaca ccctgtaacg agaattggga
     4621 aaagatgaca cctatggaaa aaggtaaatt ttgtgctagt tgtgcaatgc aggttacaga
     4681 ttttacatta atgactgata aagaaatact gcaatactta tctactacac gaggtaaggt
     4741 ttgtggaaga attcatgctg accaatctaa cagggcttta ataaattatg aaaataaaaa
     4801 aagtaaaaaa atacaatgga ccattgcagg tttaatgtct ctgttatttt caatcaataa
     4861 agtaaatgct caacaaaaaa ctgtaaagga atctccaaaa tcatcttggc ttgccgccaa
     4921 tagaaatgaa gatatggtaa ctattaaaca atcagtaact gtattaaaag ataatgctaa
     4981 aaatttaaac actccatact tacatcaggt atttgctatc gaaaatagtt tagttagcaa
     5041 atcagcctct gcatttttaa ttaataataa tgataccaca atcattaaag aagtaaaaga
     5101 cacttcgtta tcattttttg ataaagcaag ttttgaacaa accaatatta ctcttgaaac
     5161 tttattccaa atctagtagt ctccctcttg ctatgtctac ggaaatatca ggaaatgtag
     5221 attatggg
//
LOCUS       DAOQHP010000131         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9014_length_5225_cov_3.704449, whole genome shotgun sequence.
ACCESSION   DAOQHP010000131 DAOQHP010000000
VERSION     DAOQHP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9014_length_5225_cov_3.704449"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLY81_05445"
     CDS             <1..496
                     /locus_tag="PLY81_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNF29704.1"
                     /translation="EGDIIVLIGEQKNDIASSEYLHKIKKIEYSDVPYFDLDEEFKIQ
                     QFIALIINKKLIQSAHDISEGGLIITLMEKGFYNNLGFSISYNTTIRKDAYWFGEAQS
                     RVVVTVANSNYESFKTFITKANIPFTQIGTVTAGEIEINNDKWDNILVWKEKYDTAIE
                     KHLN"
     gene            512..1522
                     /gene="rfaD"
                     /locus_tag="PLY81_05450"
     CDS             512..1522
                     /gene="rfaD"
                     /locus_tag="PLY81_05450"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNF29705.1"
                     /translation="MNNINTSTNILITGAAGFIGSCMASFLNSKGYKNIYLVDDFGVE
                     IKRKNWEAIQRINIIERYNLFNWLTKEKPTINFIIHLGARTDTTEFNYEVHRELNYNY
                     SIHVWQYAVQNNIPFVYASSAATYGDGSLGYEDTHEIIQKLQPLNPYGVSKNEFDKWA
                     VKQTVHPPSWAGLKFFNVYGPNEYHKGRMASVIWHSFNQIKNNGVVKLFKSHKDGFED
                     GKQLRDFIYVKDVVLVIFWMMNCMLTNQWNKTKNGLFNLGTGKARTFEDLVKATFSGL
                     DLPSKIEFIDMPEDIRDKYQYFTEANMQKLYDAGYANTFYTLEEGVTDYVKNYLTNLK
                     IY"
     gene            complement(1622..3049)
                     /gene="dnaA"
                     /locus_tag="PLY81_05455"
     CDS             complement(1622..3049)
                     /gene="dnaA"
                     /locus_tag="PLY81_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNF29706.1"
                     /translation="MAKTAETVWVNCLKIIKDIVEWQHFKTWFEPINPVELKNNVLMI
                     QVPSQFFYEYLEEHYVALLAKTLKRELGKEARLEYRIMVDSGNGKNGSMDIPTSGVKT
                     YNNNEMNFPLVIDNPVKNPFVIPGLKKMQIDPQLNSKYTFDAFIEGDCNRVARRAGKT
                     VAEKPGANSFNPLVIYGGTGLGKTHLIQAIGNEVKRTNPNKVVLYVSSEKFINQFQDH
                     SRNNAINDFIHFYQLIDVLIIDDVHFFARAEKSQDAFFAIFNHLHQSGKQLVLTSDKA
                     PKDLDGMQERLLSRFRWGLSADLQIPDYETRIEILERKMKNDGLDMPREVVKYVAYNI
                     NTNIRELEGALISLLAQSSLNKKEIDLELAKKVLRNFVKTSSKEITIDAIQKMVCEYF
                     DVPYEKLLHKTRKREIVQARQITMYLAKAFTKNSLKTIGEHFGGRDHTTVIHSCQTVK
                     DLMDTDTIFRENVMELTQKVQLAAM"
     gene            complement(3187..4125)
                     /locus_tag="PLY81_05460"
     CDS             complement(3187..4125)
                     /locus_tag="PLY81_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF29707.1"
                     /translation="MHRRKFLKETTLAGSALIASSILPFNSLYAAVKDDTKNISILHT
                     NDVHSRLEPFPMDGSRNAGLGGVAARAALIKDIRQKTENVLLLDAGDIFQGTPYFNLY
                     KGEPEIKAMSLMGYDAATMGNHDFDAGLENFANQLIHANFPIVVCNYDFNNTPMEGKY
                     LPYTIVKKGKLKIGILGVGIEGKGLIPADLFGNTIYNNPIECANNAANKLKKEGCDLI
                     ICLSHLGDKYNDDKISDEILAKETYDIDLIIGGHTHKFFKEPRKYNNRKNKITLVNQV
                     GWAGIQLGRLDYTFSKIKNKNLANSETVVIGGKSSE"
     gene            complement(4136..4870)
                     /locus_tag="PLY81_05465"
     CDS             complement(4136..4870)
                     /locus_tag="PLY81_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF29708.1"
                     /translation="MFKQIFATYFLAILLFACSTPKQNISIQFKDYRIQQSDKKDSTL
                     TAMLKPYSDNIHQSMNTVIGFSTKGLSKKQPESELGNFLADCMKIMAEEKFSQKVDAA
                     FLNYGGIRSYLPKGDITTGNIYELMPFDNLVVLQQVSGKTLNQFLNKIAERGGWPVSG
                     VKMQIKDKQAVNIFINNKPLEESATYTIANNDYIARGGDDCDMLKEIPVNNKGYLFRD
                     AIIAFIKQLTKQGKPIEWKIENRITY"
     gene            4988..>5225
                     /locus_tag="PLY81_05470"
     CDS             4988..>5225
                     /locus_tag="PLY81_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNF29709.1"
                     /translation="MSYEIVGKLVAKFDIVQRTETFKTREFVIEKSEDIGGRIITNYI
                     KFQCVQDKTTMPERFNIGDEVKVLFNIKGTKWNKN"
BASE COUNT         1785 a          762 c          745 g         1933 t
ORIGIN      
        1 tgaaggtgat attattgtat taattggaga acaaaaaaat gatattgcat ctagcgaata
       61 tttgcataaa attaaaaaga tagaatattc tgatgtacca tattttgact tagacgagga
      121 atttaaaata caacaattca ttgcattaat catcaataaa aaattaatac aatctgcaca
      181 cgatataagc gaaggtgggt tgataataac cttaatggaa aaaggctttt ataataattt
      241 aggctttagt atttcttata atactactat tcgtaaagat gcttattggt ttggagaagc
      301 acaaagtagg gtagtggtta ctgttgcaaa ttctaattat gaaagtttta aaacattcat
      361 tacaaaagca aatattccat ttacacaaat aggaacagta acagccggag aaattgaaat
      421 aaataacgat aaatgggata atattttggt ttggaaagag aagtatgata cagccattga
      481 aaagcattta aactaattaa tattttatta aatgaataat ataaacactt caaccaatat
      541 tttaattact ggtgctgcag gttttattgg tagttgtatg gcttcttttt taaatagtaa
      601 agggtataaa aatatttatt tggtagatga ctttggagta gaaataaaaa gaaaaaactg
      661 ggaagccatt caaagaatta atataattga aaggtataat ttatttaatt ggcttactaa
      721 agaaaagccc accataaact ttataataca tttaggagcc agaacagata ccacagaatt
      781 taattacgaa gttcaccgag aattaaatta taattactcc attcatgtgt ggcaatatgc
      841 agtacaaaat aatattccat ttgtttatgc atcttctgct gctacctatg gcgatggtag
      901 tttaggatat gaagataccc atgaaataat tcaaaaattg caaccattaa atccttatgg
      961 tgttagtaaa aacgaatttg ataaatgggc agtcaaacaa accgttcatc caccaagctg
     1021 ggctggctta aaatttttta atgtttacgg acctaatgaa taccataaag gaagaatggc
     1081 aagtgtaatt tggcattcat ttaatcaaat taaaaataat ggtgtagtaa aactatttaa
     1141 aagccataaa gatggttttg aagatggaaa acagctaaga gattttattt atgtaaaaga
     1201 tgttgttctg gtaatttttt ggatgatgaa ctgcatgctt actaaccaat ggaataaaac
     1261 aaaaaatggg ttatttaatt taggtacagg taaagccaga acatttgaag atttggttaa
     1321 agcaactttt tctggcttag atttaccaag caaaatagaa tttattgata tgccagaaga
     1381 tataagagat aagtatcaat attttactga agctaatatg caaaaactgt atgatgcagg
     1441 ctatgcaaat actttttata ctttagaaga aggcgtaaca gactatgtaa aaaattattt
     1501 aactaattta aaaatatatt aactgcttga attaagattt ataactacac ccttttaaca
     1561 aaactgcttc ttcattgtat tttgaagaag cagttttgat taattaaata gtataataaa
     1621 actacatagc tgcaagctgt actttttgag taagctccat tacgttttct ctaaaaatgg
     1681 tatcagtatc cattaaatcc ttcaccgttt ggcaactatg aattacagta gtgtggtctc
     1741 ttcctccaaa atgctcacca attgttttta aagaattttt agtaaatgct tttgctaagt
     1801 acatggttat ttgccttgct tgaactattt ctctttttct tgttttatgt aatagttttt
     1861 cgtatggtac atcaaaatat tcgcaaacca ttttttgaat agcatcaata gtaatttctt
     1921 tactacttgt tttaacaaag tttcttaata cttttttagc aagttctaaa tctatctctt
     1981 ttttgtttaa agaagattga gctaaaagag aaattaaagc accttctaat tcacgaatat
     2041 tggtattaat attataagca acatatttta caacttctct aggcatatct aagccatcat
     2101 tcttcatttt acgttcaaga atttcaattc ttgtttcata atctggtatt tgcaaatcgg
     2161 cacttaagcc ccaacgaaaa cggcttaata acctttcttg cataccatct aaatctttag
     2221 gagctttatc gctggttagt accaattgtt ttccactttg atgtaagtgg ttgaagattg
     2281 caaagaaagc atcctggctc ttttcagctc ttgcaaaaaa gtgaacatca tcaataatta
     2341 aaacatctat taattgataa aaatgtataa aatcattgat agcattgttt ctgctatggt
     2401 cttgaaattg attaataaac ttttcgctgc ttacataaag aactacttta tttggattag
     2461 ttctttttac ttcattacct attgcttgta ttaaatgagt ttttcctaaa ccagttccac
     2521 cataaataac taatggatta aaagaattgg ctcctggctt ttctgcaact gtttttcctg
     2581 ctcttctggc aaccctgtta caatctcctt ctataaatgc atcaaatgta tatttagaat
     2641 ttaattgtgg atctatttgc attttcttta agcccggaat tacaaatgga ttttttactg
     2701 gattatcaat aacaagcgga aaattcattt cgttattatt gtatgttttt acaccacttg
     2761 taggtatatc catacttcca tttttgccat taccgctatc taccattatt ctatattcca
     2821 atcttgcttc cttacctaat tctcttttta aagtttttgc aagtaaagca acataatgtt
     2881 cttctaggta ctcataaaag aactggctgg gtacctgaat cattaaaaca ttgttcttta
     2941 gttctaccgg attaattggc tcgaaccaag ttttaaaatg ttgccattct acaatgtctt
     3001 taataatttt aaggcaattt acccatacag tttctgcagt tttagccata attagtttac
     3061 aaattatata attagttaag aatatttttt tagctccagt ttatcaaaat tttcaaatgt
     3121 taataagttg aaaacttttt tttgaaggga gtgcaatatg tgtaataaat attgaaaaaa
     3181 aaatttttat tcactacttt ttccccctat tacaacagtt tctgaattag ccaaattttt
     3241 attttttatt ttgctaaaag tataatccaa tctacctaat tgaataccag cccaaccaac
     3301 ctgatttact aatgttattt tattttttct attattatat tttcttggtt ctttaaaaaa
     3361 tttatgtgta tgcccgccaa taattaaatc tatatcgtag gtttcttttg ctaaaatttc
     3421 atcgcttatt ttatcatcgt tgtatttatc tcctaaatgc gataaacaaa taattaaatc
     3481 gcaaccttct ttttttaatt tatttgctgc attgttagca cattcaattg ggttgttata
     3541 aatggtattg ccaaataaat cggcaggtat aagtcctttt ccttctattc caactccaag
     3601 tattcctatt tttaatttac cttttttaac tatagtgtaa ggtaaatatt tcccttccat
     3661 tggggtatta ttaaaatcat aattacaaac aactatcgga aaatttgcat gtattaactg
     3721 attggcaaaa ttttctaaac ctgcatcaaa atcatgatta cccatagtgg cagcatcata
     3781 gcccattaaa ctcattgctt taatttctgg ttcgccttta tacaaattaa aatatggagt
     3841 tccttgaaaa atatctcctg catctaacaa taaaacattt tctgtttttt gtcgaatatc
     3901 ttttattaaa gcagctctag cagctacacc tcctaagcca gcatttctac taccatccat
     3961 cggaaaaggt tctaacctgc tgtgaacatc gttggtatgt aggatagaaa tattttttgt
     4021 atcgtcttta acagctgcat acaaactatt aaatggtagt atagaggaag ctattaaagc
     4081 actaccagct aatgttgttt cttttaaaaa ttttctacgg tgcattgtta tttttttaat
     4141 aagttattct gttttctatt ttccattcta ttggtttgcc ttgtttggtt aactgtttaa
     4201 taaaagcaat aatggcatct ctaaataaat accctttatt gttaaccgga atttctttta
     4261 gcatatcgca atcatctcca ccacgtgcaa tataatcgtt atttgcaata gtgtaagtgg
     4321 cactttcttc taatggttta ttattgatga aaatatttac agcttgttta tcttttatct
     4381 gcattttaac accacttaca ggccaaccac cacgttcagc aattttattt aaaaattggt
     4441 ttaatgtttt tccgcttact tgttgcaata caactaagtt atcaaaaggc attaattcgt
     4501 aaatatttcc tgtagtaata tctcctttag gtaaataaga tcttatgcca ccatagttta
     4561 gaaatgctgc atctactttt tgagaaaatt tttcttcagc cattattttc atgcaatctg
     4621 ctaaaaaatt tcctaattca ctttctggtt gttttttaga taagcctttt gtagaaaaac
     4681 ctattaccgt attcatagat tgatgaatat tatccgaata aggtttaagc attgctgtta
     4741 atgtgctgtc ttttttatcg gattgttgta ttctataatc tttaaattga atggatatat
     4801 tttgctttgg agtggaacag gcaaataata atatagctaa aaaataggta gcaaaaattt
     4861 gtttaaacat ggtgaaattt tataaaaaat gaaagttaga tttttgctgt taataaaaca
     4921 aagttttttt atgaataaag caaaatttat taagctacct ttgtaattaa taaaaaatat
     4981 tatttatatg agttatgaaa ttgtaggaaa attagttgca aaatttgata ttgttcaacg
     5041 tacagaaact tttaaaacta gagagtttgt aattgaaaaa tcggaagaca ttggtggtag
     5101 aattattaca aactatataa aatttcaatg tgtacaagat aaaactacta tgccagaaag
     5161 atttaatatt ggtgatgaag taaaagtatt atttaatatt aaaggaacta aatggaataa
     5221 gaatg
//
LOCUS       DAOQHP010000132         5204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9074_length_5204_cov_3.887551, whole genome shotgun sequence.
ACCESSION   DAOQHP010000132 DAOQHP010000000
VERSION     DAOQHP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9074_length_5204_cov_3.887551"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..734
                     /locus_tag="PLY81_05475"
     CDS             222..734
                     /locus_tag="PLY81_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNF29710.1"
                     /translation="MRNFHNYIRRLILTVVDFFYFPFKRIMPLQTYRYAACGGFNTLL
                     GIVLYFVAFHYIFIHETVHITEQFALKRHIASDFLFSFWINFPIGFYLNRYVVFQESK
                     LPKRIQLFRYFVVLCGSIGINYFCLKLFVEIFKIYGPIAKILTAIIVIAFSYISQRNY
                     SFKTTKEGVY"
     gene            742..1053
                     /locus_tag="PLY81_05480"
     CDS             742..1053
                     /locus_tag="PLY81_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29711.1"
                     /translation="MDRIEQIRTFLQDNPKDNFLRHALALELIKIGKDSEAKTLFEAI
                     LNEYPDYVGSYYHLAKLFERNQQIETAKEWYLKGMEVSKKAKDQHSYNELQAAYEDLV
                     Y"
     gene            complement(1116..2534)
                     /locus_tag="PLY81_05485"
     CDS             complement(1116..2534)
                     /locus_tag="PLY81_05485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNF29712.1"
                     /translation="MSDQTYSFFQSVEKSFDKAAKFTKWDQGLLEQIKACNSVYRMRF
                     PVKMDDGRIEVIEAYRVQHSQHKSPCKGGIRFSDEVNQDEVMALAALMTYKCAIVNVP
                     FGGGKGGIKINPHKYSAYELEKITRRYTAELVKKNFIGPGIDVPAPDYGTGEREMAWI
                     VDTYASLKPGEIDAAGCVTGKPISQGGVRGRKGATGLGVFFGVREVCNMPDIMKKLGL
                     TIGIEGKTVVVQGLGNVGFHSAKYFREAGAKVVAIAEYNGAIYNANGLNEEECFEYKK
                     KNGSLLGFPGATDIPNSNDALELECDILIPAALENVINKNNADRVKAKMIGEAANGPL
                     TPEADDVFNAKGILVIPDMYLNAGGVTVSYFEWLKNLSHVRYGRMEKRFDENQNEKII
                     RQIESLTGKAISDAERHQIVHGADEVDLVQSGLEETMITATQEIMEIWKANPEIPDMR
                     TAAYVNAINKVATSYSELGIFP"
     gene            complement(2584..2802)
                     /locus_tag="PLY81_05490"
     CDS             complement(2584..2802)
                     /locus_tag="PLY81_05490"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNF29713.1"
                     /translation="MGRIKLIASLCIILFSCYVVNAQELITEESRKAARNEQSIYIVM
                     AVVLTLMLGIFLYLFNLDKKLSKLEKEG"
     gene            complement(2795..3484)
                     /gene="ccsA"
                     /locus_tag="PLY81_05495"
     CDS             complement(2795..3484)
                     /gene="ccsA"
                     /locus_tag="PLY81_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF29714.1"
                     /translation="MIRKSWWKLFAFILLMYTCSMGFLVKIPQQLPGVPLFHSIRNVF
                     FHVPMWFAMMIFFTVSLIYAIKYLIKPSTKHDIYSAIYAKTGVLFGVLGIITGAIWAN
                     FQWGAYWSGDPKQNGAAVALLIYFAYFILRGSLTDEEKKARVGAVYNIFAFFMLFPTL
                     WILPRMVESLHPGGQGGSGGNPALNGGDMSAEMRMVFWPAVIGFTLLGVWMSTLYIRF
                     QILKDKKLNYG"
     gene            3671..5161
                     /locus_tag="PLY81_05500"
     CDS             3671..5161
                     /locus_tag="PLY81_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29715.1"
                     /translation="MKYWLLFFFSVTTFFVTNAQKVFEFNSTCQQAYQEITQLKLNNG
                     QVLINKAKQQNPDNLIPLVLESYIDFFTLFFNENPNDFKIRYPNFEKRISALEEGPKS
                     SPFYDFCLSTVYLHKSAVNIKFNNKTRAGFDFQAAYKLIKQNLKAYPTFTPNHLLFGS
                     LQAVAGTIPKGYQWIAGLFGIKGSITKGLKTVNHFVNNPYDPWSKLMFNEAAFIYCYL
                     MFYIENKKDEALEFIKSKKLDVVNNHLFSYMAANLSLNNKQINNAAIILQNRNKSGDY
                     LNTPVWDFQMGFVCLYRMQTQQAINYFENYLSQFKGNFYVKDIYQKLSWCYYLQGNMA
                     KAEDARKKCINRGTQVTEADKQAYKDAKKNKWANIVLLKARILNDGGFNNEAIKVLHG
                     FSTSSFKDEAEQLEFNYRVGRINDDLGKEEDAIAFYLKAITIGEHRTEYYAARAALQI
                     GMIHEKKGNKKLAITYYEKCMDMENHDYKNSLDQRAKSGIARCYGN"
BASE COUNT         1810 a          866 c          713 g         1815 t
ORIGIN      
        1 gtttaagctg tttaactaac ccaaataaaa caatagtgta atctccagta aactcttgtt
       61 tggttttatt tatcaatact tcattaggtg ctaattccaa atcaaataat tcttttatag
      121 ctgttgtaat tgtatatttt atgttttcta ttatttgcat accacttaaa cttttgggtg
      181 caaagctaat aatttacaca ctatcttagc agccttgttt tatgcgtaat tttcataact
      241 atattagaag gcttatttta acagtggttg atttttttta ttttcctttc aaaagaataa
      301 tgcctttaca aacttataga tatgcagctt gtgggggctt taatactttg ttgggtatag
      361 ttttgtactt tgttgctttt cattatattt ttattcatga aacagttcat attaccgaac
      421 aatttgcttt aaaaaggcat atagcctcag attttttatt ttcattttgg attaattttc
      481 ctataggctt ttatttaaat agatatgttg tttttcaaga gtctaaatta cctaagagga
      541 tacagctttt tagatatttt gtggtgttat gtggctctat aggaattaac tacttttgtt
      601 taaaattatt tgtagaaatt tttaaaattt atggcccaat agctaaaatt ttaacagcaa
      661 ttattgtaat tgcttttagc tatatttctc aacgtaatta ttcatttaaa actactaaag
      721 aaggagtata ttaaaaatta tatggataga attgaacaaa taagaacctt tttgcaagac
      781 aaccctaaag ataatttttt acgccatgca ttagctttag aattaataaa aattggtaaa
      841 gattctgaag cgaaaacttt atttgaagca attttaaatg agtatccaga ttatgtaggc
      901 agctattatc atttagctaa attatttgaa agaaaccagc aaattgaaac agcaaaagaa
      961 tggtatttaa aaggaatgga agtatctaaa aaagcaaaag atcagcatag ttataacgaa
     1021 ctacaagcag catacgaaga cttagtgtat taaccataaa attaaacccc gaaaaatatc
     1081 ggggtttaat tttataacaa taattcttaa atgtactaag gaaaaattcc tagttcggag
     1141 tagctcgtag caactttatt aattgcattt acataagctg ctgtacgcat atctggaatt
     1201 tcaggattgg ctttccaaat ttccataatt tcttgtgttg cggttatcat tgtttcttct
     1261 aatccacttt gtactaaatc aacttcatct gcaccatgta caatttgatg tctttctgca
     1321 tcactaattg ccttgcctgt taacgattca atttgtctga taattttttc attttgattt
     1381 tcatcaaatc ttttctccat tctgccataa cgaacgtggc taagattttt taaccattca
     1441 aaatacgaaa cggttacacc gcctgcattt aaatacatat ctggaattac taaaattcct
     1501 tttgcattaa aaacatcgtc agcttctgga gttaatgggc catttgcagc ttcgccaatc
     1561 attttagctt ttactctatc tgcattattt ttattaataa cattttctaa agctgcagga
     1621 attaatatat cacattctaa ttctaaagca tcattactat taggtatatc tgttgcacca
     1681 ggaaagccta ataaagaacc atttttcttt ttatattcaa aacactcttc ttcgtttaag
     1741 ccattggcat tataaatggc tccattatat tctgcaatag caactacttt tgcacctgct
     1801 tctctaaagt attttgcact atgaaatcct acattaccca aaccttgtac aacaactgtt
     1861 ttgccttcaa taccaatagt taaaccaagt tttttcataa tatctggcat attacatact
     1921 tctcttacac caaaaaatac gcctaaccct gtagcccctt ttcttcctct aacacctcct
     1981 tgagaaatag gtttaccagt tacacaacct gctgcatcaa tttcgcctgg tttaagtgaa
     2041 gcataggtat ctacaatcca tgccatttct ctttcgcctg ttccataatc tggtgcaggt
     2101 acatctatac cagggccaat aaagtttttc ttcactaatt cagcagtata acgtcgagta
     2161 attttttcta actcgtatgc actgtattta tgtgggttaa ttttaatacc acctttacct
     2221 ccaccaaatg gtacatttac aattgcacat ttgtaggtca ttaaggcagc tagagccatt
     2281 acttcatctt ggtttacttc atcgctaaat cttataccac ctttacatgg acttttgtgc
     2341 tggctatgct gcactctata ggcttcaatt acttcaattc tgccatcatc cattttcact
     2401 ggaaaacgca ttctgtaaac actgttacaa gctttaattt gttctaataa tccttggtcc
     2461 cacttagtaa attttgccgc tttatcaaag cttttttcta cgctttgaaa aaaactgtaa
     2521 gtttgatctg acataagttt ttaattttta attaagcaat cattattatt tataaataaa
     2581 gttttatccc tctttttcta gtttagataa ctttttatcc aaattaaata aatacaaaaa
     2641 tattccgagc attaaagtta aaacaactgc cattacaata tatatgcttt gttcatttct
     2701 tgctgctttt ctactttctt ctgtaatcaa ttcttgtgca ttaactacat aacaagaaaa
     2761 caaaattata cataaactgg ctattaattt tattctaccc ataattcaat ttcttatctt
     2821 ttaatatttg aaacctaata tataaagtac tcatccaaac acctaacaaa gtaaacccaa
     2881 taactgctgg ccaaaaaacc attctcattt ctgcactcat atcgcctcca tttagagcag
     2941 gattacctcc tgagcctcct tgcccaccag gatgtaaact ttctaccatt cttggtaata
     3001 tccaaagtgt tggaaacaac ataaaaaatg caaaaatatt atacacagcg ccaacccttg
     3061 ctttcttttc ttcatcagtt aaacttcctc ttaatataaa ataagcaaag taaattaata
     3121 aggcaacagc agctccgttt tgtttggggt ctccgctcca atatgctccc cattgaaagt
     3181 tagcccaaat tgctccagtt ataattccta aaacaccaaa tagaactcct gtttttgcat
     3241 agatagctga ataaatatcg tgtttagtag aaggtttaat taaatatttg atagcataaa
     3301 ttaatgatac tgtaaaaaat atcatcattg caaaccacat tggaacatga aagaaaacat
     3361 ttctaataga atgaaataat ggaacaccag gtaattgttg aggaattttc acaagaaagc
     3421 ccatactgca tgtgtacatt agtaaaataa atgcaaatag tttccaccaa gactttctaa
     3481 tcataactga tgataccaaa taatggtttg caaatgtagg ataaagccca acactaaaaa
     3541 aagtaaaatt taacttaatt gctcaatagt tactaactct tctatatttc aataaaaaat
     3601 attccttata aaattgctaa gaaaagtcaa aaaattactt tattaaagtt gcatcaaata
     3661 catttgaggc atgaaatatt ggttactatt tttttttagt gttacaactt tttttgttac
     3721 aaatgcccaa aaagtatttg aatttaatag cacttgccaa caggcttatc aggaaattac
     3781 acaactgaaa ttaaacaatg gacaagtatt aattaataaa gccaaacaac aaaatccaga
     3841 taatcttata cctttagttt tagaaagcta tattgatttt tttactctat tttttaatga
     3901 aaatccaaac gattttaaaa taagatatcc caattttgaa aaaagaatta gtgccttgga
     3961 agaagggcct aaaagctctc ctttttatga cttttgttta agtactgttt atttacacaa
     4021 atcggctgtt aatattaaat ttaataataa aacaagagca ggttttgatt ttcaggcagc
     4081 ttataagcta attaaacaaa atcttaaagc ctatcctact tttaccccaa accatttatt
     4141 atttggcagc ttacaagctg ttgctggtac tattccaaaa ggttaccaat ggattgcagg
     4201 tttatttgga attaaaggta gtattacaaa aggactaaaa acggttaacc attttgtaaa
     4261 caacccttac gacccatggt ctaaattaat gtttaacgaa gctgctttta tttactgtta
     4321 tttaatgttt tatatagaaa ataaaaagga tgaagcttta gagtttatta aaagtaaaaa
     4381 attagatgtt gtaaataatc acttgtttag ctacatggct gctaacctaa gtttaaataa
     4441 taaacaaata aataatgccg ccattatttt acaaaacaga aataaatcgg gagattattt
     4501 aaatactcca gtatgggatt ttcagatggg atttgtatgt ttatacagaa tgcaaaccca
     4561 acaggcaatt aattattttg agaactattt atctcaattt aaaggaaatt tttatgttaa
     4621 agatatttac caaaaactaa gttggtgcta ctatttacaa ggcaatatgg ctaaagcaga
     4681 agatgccaga aaaaaatgta tcaatagagg aacacaagtt acagaggctg acaaacaagc
     4741 ctataaagat gccaagaaaa acaaatgggc aaacatagta ttacttaaag ccagaatatt
     4801 aaatgatggt ggttttaata acgaggcaat aaaagtttta catggcttta gtacttcaag
     4861 ttttaaagac gaagcggaac aattagagtt taattataga gttggcagaa taaatgacga
     4921 cttaggtaaa gaggaagatg ctattgcttt ttatttaaaa gctattacaa ttggagaaca
     4981 tagaactgaa tattacgctg ctagggctgc tttacaaatt ggtatgatac atgagaaaaa
     5041 aggtaataaa aaattagcta tcacctatta cgaaaaatgt atggatatgg agaaccatga
     5101 ttataaaaac agcttagacc aaagggcaaa aagtggcatt gcacgttgtt atggaaatta
     5161 aagtttcctc acttaatcat cattaataat aaatatcttt taac
//
LOCUS       DAOQHP010000133         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9138_length_5179_cov_3.836651, whole genome shotgun sequence.
ACCESSION   DAOQHP010000133 DAOQHP010000000
VERSION     DAOQHP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9138_length_5179_cov_3.836651"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1721)
                     /locus_tag="PLY81_05505"
     CDS             complement(84..1721)
                     /locus_tag="PLY81_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF29716.1"
                     /translation="MIEFFQHFMSEIEIPLKNPVLVFSLILLIILLSPLLLKKINIPG
                     IIGLIIAGVVIGPHGFNILAKNSAIDLFSTIGLLYIMFIAGLELDLNEFKANKNKSLL
                     FGFFTFIIPLSIGFPVCYYFLGYNFNTSLLTASMFATHTLVAYPIVSKFGVSKNQAVA
                     ITVGGTILTDTAVLIILAVIMGNAQGSLNNEFWIKLGVSLSIFSAIMFLLVPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVLLKGPTAIIVAATLSVVALFSKWFAALFTQLIFK
                     YSKAQRQLIFGLSGAHAAATLAVILVGFKAGILDENILNGTIILILITCIVASFATEK
                     AAKKILIETDEDASNIVKPNGVENEHILLPISNFSNIDKLLEFSGLIKEKKSYHPISM
                     LSVVSDNNEAEANLIKARNKLEEFVKQASASETKVKIITTLDHNDSSGINRISKEIMA
                     DIIILQWSERIGFFDKFLGDKTDNIIIPHHIDVDICS"
     gene            2078..2578
                     /locus_tag="PLY81_05510"
     CDS             2078..2578
                     /locus_tag="PLY81_05510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNF29717.1"
                     /translation="MQELYYTYYESPIGLIRIGGTDNFIAELVFIDNKDQIKHGEPGL
                     SDLMHVCTEQLIEFFAGKRKQFNVPIHQEGTAFQGNVWSELIHIPFGSTISYLDLAKK
                     IGDAKATRAVAAANGKNNIAIIVPCHRVIGSDKSLTGYAGGMWRKKWLLQHEFRIKNG
                     IQVLFN"
     gene            2694..3902
                     /locus_tag="PLY81_05515"
     CDS             2694..3902
                     /locus_tag="PLY81_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29718.1"
                     /translation="MKKTFLPILLLSALFVNQSSAQSNKAYAITAETKGNYNWTAIRE
                     IDLNNGNVIRTLYSNSNKPKVSYEFINNDWQANRVNIQSMSPTVNGVAAAAFDEKNNR
                     LYFTPMWGTDLRYVDLNTTEMNVVVNGDARFSTGKRVDESNVITRMVIAADGNGYALT
                     NDANQLIQFTTDKNPTITNLGALVDSKKNNGISVHTQCTSWGGDLIADVYGNLYLITF
                     RNHIFKINPTSKIAEHIGTIKGLPENFTTNGAAIDANGEVFISSAIQTNNYYKVNLST
                     LNASVIVKEGNETVYNASDLANSNLAYRNTRNNIVPTTVKNNGIAIYPNPVVNKTFAI
                     QFEKLPAGNYGVEITDVNGKRILNKTINIIGLQNEKINLPRASASGLYFVKITNTAGK
                     AIFTDKIVVE"
     gene            complement(4003..>5179)
                     /locus_tag="PLY81_05520"
     CDS             complement(4003..>5179)
                     /locus_tag="PLY81_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF29719.1"
                     /translation="KELSPETRVIVISGQEDVKVAINLLKKGAYDYIVKDEDTKDRLW
                     NSIQHLRENADLKKEIEVLKEEVAKKYDFAKTIIGNSIAIKRVFNLMEKAVKNNITVS
                     LTGETGTGKEVAAKCIHYNSDRRNKSFVAINVAAIPKELLESELFGHEKGAFTGAAVR
                     RIGKFEEANKGTLFLDEIGEMDMNMQSKLLRALQERELSRVGSNEIIKFDVRIIVATH
                     KNLLDEVKNGNFREDLYYRLLGLPINLPPLRERENDVLMIAKFYMEQFCKENKIPSKV
                     LTTEAKKKLLNHSFPGNIRELKSVIELATVMAEDTEILPEHLNIVVQNVMENFAIKDA
                     TLKEFNVKLIQYYLDLYQCDVLKVAKKLDIGKSTIYRMIQNKELSVSKNVLDETIMN"
BASE COUNT         1849 a          837 c          711 g         1782 t
ORIGIN      
        1 ggagatgatt atgtatcttc cacattattt tcctgtctga tttggatcgc tgtttggacg
       61 cttgtagttc tcaaagatag tatttatgag catatatcta catcaatatg gtgtggaatg
      121 atgatattat ctgttttatc tcctaaaaat ttatcaaaga aacctattcg ttctgaccat
      181 tgtaaaataa ttatatctgc cattatttct tttgaaattc tattaattcc gctagaatcg
      241 ttatgatcta aagtagtaat aatttttact tttgtttcag atgcagatgc ttgttttaca
      301 aattcttcca gtttgtttct tgctttaatt aaattagctt ctgcttcatt gttatctgac
      361 acaacagata acatagatat aggatgatag gattttttct ctttaattaa tccagaaaat
      421 tctaagagtt tatcaatatt agaaaagttt gaaatgggta atagaatatg ttcattctct
      481 accccattgg gtttaactat attacttgca tcttcatctg tttcaattaa tattttttta
      541 gcagcttttt cagtagcaaa tgaggcaaca atacaagtaa taagaataag aataatagta
      601 ccatttaaaa tgttttcatc taaaatacct gctttaaagc ctactaaaat tactgctaaa
      661 gtagcagcag catgtgcccc acttaatcca aaaattaatt gccgttgagc tttagaatat
      721 ttaaaaataa gttgcgtgaa taatgctgca aaccatttgc tgaataaagc aaccaccgat
      781 aatgtagctg ccacaattat tgcagttggc ccttttaata atacacttac atctaccaac
      841 attcctacac ttattaaaaa gaaaggtata aaaagtgaat taccaataaa ctcaattctg
      901 ttcattaagg ccgaagagtg aggtattaat ttatttaatg ccagcccagc cacaaaggca
      961 ccaattattg gttcaacacc agcaacctcg gccaaaaaag cagcaaaaaa aacaactgaa
     1021 agaacaaaaa tataatgaga gtgtttttcg ctttctaatt ttctgaagaa ccattttgca
     1081 atacgtggta ctaataaaaa cataatggca gaaaatatac ttaatgaaac acccaacttt
     1141 atccaaaatt cattgtttaa gctaccttgt gcattaccca taattacagc caaaataatt
     1201 agcactgcag tatctgttaa aatagtacca ccaacagtta tagccactgc ctggtttttt
     1261 gaaacaccaa acttgctaac tattggatag gctaccaaag tatgtgttgc aaacatactt
     1321 gcagtaagta aacttgtatt aaaattatat cctaaaaaat aataacaaac aggaaagcca
     1381 atggaaagtg gaataataaa tgtaaaaaaa ccaaagagca aacttttgtt tttgttggct
     1441 ttaaactcat tcaaatctaa ttctaagcca gcaataaaca taatgtataa taagccaatg
     1501 gtagaaaata aatcaatagc agagtttttg gctaaaatat taaatccatg aggaccaatt
     1561 acaacacctg ctataattaa cccaattata cctggaatgt taattttttt taataacaat
     1621 ggagataata aaataattaa cagtattaat gaaaaaacta aaactggatt tttcaatggt
     1681 atttctattt ctgacatgaa gtgttggaaa aattcaatca tagcttacat tttataaatt
     1741 atagagaatg aaaaaacgaa tcatccactc ttacaataat acaaaaaatt ttaaagcaac
     1801 cttaaataag tcattaacta ttggttaaag aaaagtttca tgaaacaatc ttcatgaact
     1861 gtttccaaac tttatatttg ttttatactt tcatctcgta tataatgtaa agcgtgtggt
     1921 ttgattataa tttatactat cataaattgg gagcctaaca aactaaaagt atagcttata
     1981 aaactttttg caactctcct tattaaatgc tattacttta caacaattat tttatttact
     2041 tcaattcgta taattttacc agcagcaaaa aagcaatatg caggaactgt attatacata
     2101 ctacgaaagt cccattggcc tgattagaat tggtggtacc gataacttta ttgcagaatt
     2161 agtttttatt gacaacaaag atcaaattaa acatggcgaa cctggcttga gtgatttgat
     2221 gcatgtttgt actgaacaat taattgaatt ttttgcagga aaaagaaaac aatttaatgt
     2281 ccccattcat caagaaggaa cagcttttca aggaaatgtt tggagtgaat taattcatat
     2341 accttttggt agtaccatta gttatttaga tttagctaaa aaaattggag acgcaaaagc
     2401 aacaagagca gtagctgcag ccaatggcaa aaacaatatt gctattattg tgccttgtca
     2461 cagagtaatt ggtagcgata aaagcctaac aggttatgct ggtggtatgt ggcgaaaaaa
     2521 atggttatta caacatgaat ttagaataaa aaacggcata caggttttat tcaactaata
     2581 gtactttttc atttttcttc tttactattt gttaaatatt tctatttatc aaaaaatcat
     2641 tgaatattat ttttttcttc ctaaatttaa ccaacaattt aaactttaca actatgaaga
     2701 aaacattttt acccatctta ttactctctg ctttatttgt aaatcaatct agtgctcaaa
     2761 gtaataaagc atatgctatt actgctgaaa ctaaaggaaa ttataattgg acagcaataa
     2821 gagaaattga tttaaataat ggtaacgtta ttagaacttt atacagtaat tccaataagc
     2881 ctaaagtaag ttatgagttt attaataacg attggcaagc aaacagagta aatatacaaa
     2941 gcatgtctcc tacggtaaat ggcgttgctg ctgctgcttt tgatgaaaaa aataatcgtt
     3001 tatattttac gccaatgtgg ggtaccgatt tacgttatgt agatttaaac acaacagaaa
     3061 tgaacgttgt agttaatggc gatgcaagat tttctacagg caaaagagtg gatgaaagca
     3121 atgtaattac aagaatggta attgctgcag atggcaatgg ttatgcactc acaaacgatg
     3181 caaaccaact aattcaattt actactgata aaaatcctac aatcactaat ttaggagcat
     3241 tagtagatag caaaaaaaat aatggtatta gtgttcatac tcaatgcaca agttggggtg
     3301 gcgatttaat agcagatgtt tatggaaact tatacttaat tacttttaga aaccatattt
     3361 ttaaaattaa tccaacttca aaaattgcag aacatatagg taccattaaa ggattaccag
     3421 aaaactttac aaccaatggt gcagcaatag atgctaatgg agaggtgttt atttctagtg
     3481 ctatacaaac aaataattac tacaaagtaa acttatctac cttaaatgct tctgtaatag
     3541 taaaagaagg caacgaaact gtttacaatg catctgactt agcaaatagt aatttggctt
     3601 acagaaatac aagaaacaat attgtaccaa caacagtaaa aaataatggc attgcaattt
     3661 atcctaaccc ggttgtaaat aaaacttttg caattcagtt cgaaaaattg cctgcaggta
     3721 attatggagt tgaaataact gatgtaaacg gtaaaagaat tttaaataaa acaattaata
     3781 ttattggttt acaaaacgaa aaaattaacc taccaagggc ttcagcatct ggtttatatt
     3841 ttgtaaaaat caccaatact gccggtaaag ctatatttac cgataaaatt gtagttgaat
     3901 aaattttcat tacccttatt taatttatta aacctatcaa cgataaatgc cacctttata
     3961 agggtggcat tttttcattg aaccttggat tttaaaatac tatcaattca taattgtttc
     4021 gtctaaaaca tttttactaa ctgataattc tttgttttga atcatacggt aaatagtact
     4081 cttacctatg tcaagcttct tagcaacctt taatacatcg cattgataaa ggtccaaata
     4141 atattgtatt aatttaacat taaattcttt taaagtagca tcttttatgg caaaattctc
     4201 cattacattt tgtacaacaa tattcaaatg ctcaggtaat atttctgtat cttctgccat
     4261 tactgttgca agctcaatta ctgattttag ctcacgaata tttccaggaa aactatgatt
     4321 taataatttc ttttttgcct ctgtagttaa tactttagag ggtattttgt tttctttaca
     4381 aaattgctcc atataaaatt ttgcaatcat caacacatca ttttctcttt ctcttaaagg
     4441 tggcaaatta attggcaagc ctaaaagcct ataatacaaa tcttctctaa aattaccatt
     4501 tttaacttca tctaataagt ttttatgagt agcaacaatt attcttacat caaattttat
     4561 aatttcgtta ctccctactc tactaagttc tctttcttgt aaagccctta ataatttaga
     4621 ttgcatattc atatccatct caccaatttc atctaaaaat aaagttcctt tattggcttc
     4681 ttcaaatttt ccaattcttc ttacagcagc acctgtaaaa gccccttttt cgtgtccaaa
     4741 aagttcactt tctaaaagct ccttgggtat agctgctacg ttaatagcaa caaaggattt
     4801 atttcttcta tctgaattgt aatgaatgca tttagctgca acttctttac ctgtacctgt
     4861 ttcgccagtt aaggatactg taatattgtt tttaacagct ttttccatta aattaaaaac
     4921 tctcttaatg gcaatactgt ttccaattat agtttttgca aagtcatatt ttttggcaac
     4981 ttcttctttt aaaacttcaa tttccttttt aagatctgca ttttctctta aatgttgaat
     5041 gctattccaa agcctgtctt tggtatcttc atcttttaca atataatcat aagcaccttt
     5101 cttaagtaag ttaatagcaa ctttcacatc ttcctgacca gatattacaa ttactctagt
     5161 ttcggggctt agctcctta
//
LOCUS       DAOQHP010000134         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9282_length_5126_cov_4.211004, whole genome shotgun sequence.
ACCESSION   DAOQHP010000134 DAOQHP010000000
VERSION     DAOQHP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9282_length_5126_cov_4.211004"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="obgE"
                     /locus_tag="PLY81_05525"
     CDS             complement(<1..750)
                     /gene="obgE"
                     /locus_tag="PLY81_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNF29720.1"
                     /translation="MSEKNNFIDYIRIFCRSGHGGAGSRHFLRNKLTAKGGPDGGDGG
                     RGGHIILRGNAQLWTLLHLRFYKNVLAQNGENGSKNNCTGKDGEDIIIEVPLGTIARD
                     EETRLIEAEILEDKQEIVWLKGGRGGLGNQHFATPTNQVPEHAQPGEEGVEGWKQLEL
                     KVLADVGLVGFPNAGKSTLLSVITAAKPKIANYAFTTLKPQLGMVDYRDGKSFCVADL
                     PGIIEGAAEGKGLGHRFLRHIERNSCLLFVIP"
     gene            complement(830..2473)
                     /locus_tag="PLY81_05530"
     CDS             complement(830..2473)
                     /locus_tag="PLY81_05530"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HNF29721.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKQVGDLVNVGEVLAEIETDKA
                     TMELESYKQGTLLHIGTPNGGKLQVNDLLAIIGNKEEDIAALIAQHSLGNKTANTTTV
                     ILPTTNESISKETIQTPTPSSKVNFDEVVLMPRLSDTMTEGVIASWQKNIGDTVKKGE
                     VLAEIETDKATMELESYKDGILLYHGANAGEKIQVNDLLCIIGSNATDVAAILASVKN
                     GSHVATAATTSQPAIPLDSNANTTSTSSNNTQTNVNEGRVFASPLAKKIAIDKGIDLK
                     NVKGSGDNGRIIKSDIETYQPPQATTSLNITETKTTTPQFTATGVESFNEVPVSQMRK
                     TIARRLGESKFTAPHFYLTMRIDMDAAVEARTKLNADGKTKISFNDMVLKAVAIALKQ
                     HPKVNSSWLEDKIRYNHHINIGVAVAVEEGLLVPVVRFADTLSLTQISSIVKDYAQKA
                     KDKKLQPSDWEGNTFTISNLGMYGIDQFTAIINPPDACILAVGGIAQVPVVKNGMVVP
                     GNVMKVTLSCDHRVVDGAIGSAFLQTLKSLLEEPLRMML"
     gene            complement(2662..4791)
                     /locus_tag="PLY81_05535"
     CDS             complement(2662..4791)
                     /locus_tag="PLY81_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNF29722.1"
                     /translation="MEKVEMKVEGMSCTNCALTIDKYLKEQGLQNVKVNFIGGDVSFD
                     MLDNTSVEAIEKGIYKLGYTVKNDHQNSNSKQTYYGFLPFKNNLQRFWFCFVFTLPLM
                     LHMIPFLHIHFLMNPYVQLGLTIPVYVVGMDFFARSAIKSMQKGIPNMNVLITIGSTA
                     AFIYSLYGLLIGKAQDYLFFETAATTITLVFLGNYMEDKSVEQTQAALKKLAVSQKTM
                     ANMIAFDDKHEEQIFQVESKDLRVGDLVLIRNGEFVPMDCKILWGEAEVNEAIITGES
                     IPVRKATKDKLIGGSIVEAGTVKAQITAVGQDTVLANILKMIKNAETEKPPVQQMADK
                     ISAIFVPTVVSIALLTLLINYFFASQTFAESLLRSIAVLVISCPCAMGLATPAAIAVG
                     LGRGAKKGILFKNARSLEIFKSITQVVFDKTGTLTTGKFSIAQFHSTIDENDFKTLVY
                     SLEKYSNHPIGKSIAAEWKTKNDIRWSAIEEVKGLGMKATDKEGNEFIAGSFKTSNQT
                     TPQEELHNVYVSKNGKLIGWIDVKDELRPEAAQVVQILKQKGIKTILLSGDKTEKCVE
                     VQKTLGIDEIIAEQTPEQKLQHIDRLNKLSPTAMVGDGINDGPALAKATVGISLSDAS
                     QVAMQSAQVVLMNHGLKNLPEALGLGKHTYITIKENLFWAFSYNIVAIPVAALGFLSP
                     TFGALIMGLSDVVLAVNSLRLKWKKVM"
     gene            4887..>5126
                     /locus_tag="PLY81_05540"
     CDS             4887..>5126
                     /locus_tag="PLY81_05540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNF29723.1"
                     /translation="MDAIFTLQTINEVAAMFWKRYQQRKVWAFHAAMGTGKTTFIHSL
                     CNHVLNIKENISSPTFSIINEYNSPIAGTVFHTDWY"
BASE COUNT         1545 a          967 c          708 g         1906 t
ORIGIN      
        1 gggtattaca aaaagcaaac aactgtttct ttcaatatgc cttaaaaatc tatgtcctaa
       61 gccttttcct tctgcagcac cttcaataat gcctggcaaa tctgctacac aaaaactttt
      121 cccatctcta taatctacca ttcctaattg tggctttaat gtggtgaaag catagttagc
      181 aattttaggt ttggcagcag taattacact taataaagtt gatttgcctg catttggaaa
      241 accaaccaaa cctacatctg ccaatacttt cagttctaat tgcttccaac cttctacacc
      301 ttcttctcct ggctgtgcat gctctggcac ttgattggtt ggtgttgcaa aatgctgatt
      361 tcctaaacca cctctaccgc cttttagcca aacaatttct tgtttatctt ctaatatttc
      421 tgcctctatc aatctggttt cttcatctct agcaattgtt cctaatggca cttcaattat
      481 aatatcttct ccatctttac ctgtacaatt atttttactt ccattctctc cgttttgtgc
      541 tagcacattt ttataaaatc ttaaatgcag taatgtccaa agctgtgcat tacctcttaa
      601 aataatatgt cctcctctgc caccatcacc tccatctggc ccacctttag cagttaattt
      661 atttcgtaaa aaatgtcttg aaccggcacc tccatgccca cttctacaaa atattcttat
      721 ataatctata aaattatttt tttccgacac gttacaattt aattatgttt taaaatttta
      781 cggcgtagaa aaaactacgc cgcaaagaat tattttttat acctattttt tataacatca
      841 ttcttaaagg ctcttctaac aaacttttta atgtttgtaa aaatgcagag ccaatagcac
      901 catctacaac tctatggtcg caacttaaag taactttcat tacattacca ggtacaacca
      961 ttccattttt tacaactggc acttgtgcaa taccgccaac tgctaaaata caggcatctg
     1021 gtggattaat aattgcagta aattggtcaa taccatacat tcctaaatta ctaattgtaa
     1081 aagtatttcc ttcccaatca gatggttgaa gttttttgtc tttagccttt tgggcatagt
     1141 cttttacaat ggaagaaatt tgggttaaac ttaacgtatc tgcaaagcgt acaacaggta
     1201 ctaataaacc ttcctctaca gctacagcaa cgccaatatt aatatgatgg ttataacgta
     1261 ttttatcttc gagccaactg ctattaactt ttggatgttg ttttaaagca atagcaactg
     1321 cttttaaaac catatcatta aagctaattt ttgttttgcc atctgcattt aatttagttc
     1381 tggcttccac agcagcatcc atatcaatac gcattgttaa gtaaaaatga ggggcagtaa
     1441 atttgctttc ccctaatctt cttgcaatgg ttttacgcat ttggctaact ggtacttcat
     1501 taaaactttc tacgccagta gcagtaaatt gtggagttgt agttttcgtt tctgtaatat
     1561 ttaaactggt tgtagcctgt ggtggttgat aggtttcaat atctgatttt attattctac
     1621 cattatcgcc actacctttt acatttttta aatctatgcc tttatctata gcaatttttt
     1681 tagccaatgg cgaagcaaaa actcttcctt catttacatt agtttgtgta ttattggatg
     1741 aagtagaagt tgtatttgca ttactatcta atggtatagc aggttgagaa gttgtggcag
     1801 cagttgctac gtggctacca ttttttacag atgcaagaat tgcagcaaca tctgttgcat
     1861 tgctacctat aatacaaagt aaatcattta cctgaatttt ttctcctgca tttgcaccat
     1921 gatacagcaa aattccatct ttatagcttt ctaattccat agtggcttta tctgtctcaa
     1981 tctctgctaa cacttctcct ttcttaactg tatcaccaat atttttttgc caacttgcaa
     2041 ttacgccttc tgtcatagta tcgcttaaac gaggcattaa aactacttca tcaaagttta
     2101 cttttgatga tggtgttggt gtttgtatcg tttcttttga aatgctttca ttagtagtag
     2161 gcaaaataac agttgtagta tttgcagttt tgttacccaa actgtgttgt gctattaatg
     2221 cagcaatatc ttcttcttta tttccaataa tggcaagcaa atcatttact tgaagtttgc
     2281 caccatttgg tgtaccaata tgtaacaaag tgccttgttt gtagctctct aattccattg
     2341 ttgctttatc agtttcaatc tctgcaagta cttctcctac atttaccaaa tcgccaactt
     2401 gtttgtgcca agctgctatt acaccttcag tcatggtatc gctaaggcga ggcattaaaa
     2461 taacttcagc cataaaatat tttaattata cttttttaat cttttagagc cgtgaaaata
     2521 cagtaaaata aagttttgac aagtattcct caaatttttg ttattaaaaa tcaacaaata
     2581 gaaataaata gctcaatttt taataaaatt tgaaagaaat aaaacctgtt ttttaaagtt
     2641 ttgactatta ggatgaaaaa cttacattac ttttttccat tttaatctta aactatttac
     2701 tgctaaaaca acatcactta agcccattat taaagcccca aaagttggac ttaaaaagcc
     2761 taacgcagca acaggtatgg caacaatatt ataagagaaa gcccaaaata aattttcttt
     2821 aatggtaata taggtatgtt ttcctaaacc taatgcttct ggtaaatttt ttaatccatg
     2881 attcattaat actacttgtg cactttgcat agcaacctgg cttgcatcgc ttaaagaaat
     2941 accaactgtt gcttttgcta atgcagggcc atcattaata ccatctccaa ccattgcagt
     3001 aggagaaagt ttatttaatc tatcaatatg ttgtaacttt tgttcgggtg tttgttctgc
     3061 aattatttca tctatcccta aggttttttg cacctctaca catttttctg ttttatcgcc
     3121 actaagtaaa atagttttaa ttcctttttg ttttaaaatt tgtactactt gtgcagcttc
     3181 gggccttaat tcatctttta catcaatcca tccaattaat tttccatttt tagaaacata
     3241 tacattatga agctcctctt gaggtgttgt ttgattactt gttttaaaag aaccggcaat
     3301 aaactcattt ccctctttat ctgttgcctt catacctaaa ccttttactt cttctatggc
     3361 tgaccagcgt atatcatttt ttgttttcca ctcggcagct atggatttac ctattggatg
     3421 attagaatat ttttctaagg aataaaccaa tgttttaaaa tcattttcat caatggttga
     3481 gtgaaattga gctatagaaa attttcctgt agttaaagtt cctgttttat caaatacaac
     3541 ttgggttatg gatttaaaaa tttctaagct tctggcattt ttaaatagta tgcctttttt
     3601 tgctcctcgt cctaagccaa ctgcaattgc tgcaggtgtt gctaaaccca tagcacaagg
     3661 gcaactaatt accaacactg caatactcct taacaaactt tcggcaaatg tttgcgatgc
     3721 aaaaaagtag tttattaata aggtaagtaa tgctatgctt acaactgttg gtacaaaaat
     3781 ggcactaatt ttatctgcca tttgttgtac aggtggtttt tcagtttcgg catttttaat
     3841 catttttaaa atgttagcta aaacggtatc ttgtcctact gctgtaattt gtgcttttac
     3901 tgttccagct tctacaatac tgccaccaat taatttatct ttagtagctt ttcttacagg
     3961 aatactttcg ccagttataa ttgcctcgtt tacttctgcc tcgccccata aaattttaca
     4021 atccattggt acaaattctc cattacgaat taataccaaa tctccaactc ttaaatcttt
     4081 gctttctacc tgaaaaattt gctcttcatg tttatcatca aaagctatca tattagccat
     4141 tgttttttgg ctaacagcta atttttttaa tgctgcttgt gtttgttcaa cacttttatc
     4201 ttccatataa ttacctaaaa aaactaatgt tatggttgta gcagctgttt caaaaaataa
     4261 ataatcttgg gctttgccaa taagtaaacc atataaacta taaataaatg cagcagtact
     4321 accaattgta attagcacat tcatatttgg tattcctttt tgcatgcttt ttatagcact
     4381 tcttgcaaaa aaatccattc caacaacata cacaggtatg gttaagccaa gttgtacata
     4441 agggttcatt aaaaaatgaa tatgtaaaaa tggtatcatg tgcaacatta atggcagggt
     4501 aaacacaaaa caaaaccaaa aacgttgcaa gttattttta aaaggaagaa agccatagta
     4561 agtttgtttt gaattggagt tttgatgatc attttttact gtatagccta atttatagat
     4621 gcctttttct attgcttcta ctgatgtatt atctagcata tcaaaactaa catcgcctcc
     4681 aataaaattt acttttacat tttgtaaccc ttgttctttt aaatatttat caatagttaa
     4741 agcacaattt gtgcaactca taccttctac tttcatttcc actttttcca taatttttgc
     4801 tacaattttt tattaaaatt agaatttgta aaggtactta atatgttgta gtaactatca
     4861 tattgttaag tgaatatctt tgtatcatgg atgcaatatt tacccttcaa acaataaatg
     4921 aagtagcagc aatgttttgg aaacgttatc agcaaagaaa agtatgggct tttcatgctg
     4981 ctatgggaac tggtaaaact acttttattc atagtttatg caaccatgtt ttaaatataa
     5041 aagagaatat tagtagccct acattttcta tcattaacga atataatagc cctatagctg
     5101 gtactgtttt tcatacagat tggtat
//
LOCUS       DAOQHP010000135         5119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9298_length_5119_cov_3.575632, whole genome shotgun sequence.
ACCESSION   DAOQHP010000135 DAOQHP010000000
VERSION     DAOQHP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9298_length_5119_cov_3.575632"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLY81_05545"
     CDS             complement(<1..866)
                     /locus_tag="PLY81_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF29724.1"
                     /translation="MQSNNNSFSLRFLKNKAAVFALLFIFLVLIIAIFAYLIAPENSS
                     NANRIIPEIGSKKPGFSIYLLKTKQRESAKENFFTILLNGKSDNYFYTPITEYKILKD
                     SLYYQKFIDEDITENKVIKIQGSNFEENNIIKQTFYLGTDKFGRDILSRLIIGSRVSL
                     GVGCIAAIISLTIGVFLGAVAGYFKGKTDNVIMLLINIVWSIPTLLLVFAITLVLGKG
                     FWQVFIAVGLTMWVNVARLVRGQVMIAKELEYIEAARVLGFNHFRIIVKHILPNIIGP
                     ILVITAANFAGA"
     gene            complement(880..1992)
                     /locus_tag="PLY81_05550"
     CDS             complement(880..1992)
                     /locus_tag="PLY81_05550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29725.1"
                     /translation="MAYAIVLLFYRKWFLQLKHFNLPQNYTPTTSFSIIIPARNEEAN
                     IRTCLTSILQQQYPTTLFEIIVIDDHSSDNTSLIVEELQHQYSNLKLIKLVDELDGEM
                     LNSYKKKAIENAISYAKANWIITTDADCFMQTKWLSIFDAWIQENDTVFVAAPVAFTN
                     NGSLLQTFQYIDFMSLQGITAASVNAGFHNMCNGANLAYKKEVFFEVNGFKGIDNIAS
                     GDDMLLMNKIKNHYTNGVSFLFSKQAIVTTHPMPTWSSFFNQRIRWASKAEQFKTKDT
                     SIFIVLVLVYLLNVTLFIMPFLSIFNFIFLWYWLLFIMLKTIAELAFALPVAKFFSQP
                     FIWWFPFLQPLHIVYIVVSGWLGKFGTYNWKGRNVK"
     gene            2154..3935
                     /locus_tag="PLY81_05555"
     CDS             2154..3935
                     /locus_tag="PLY81_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29726.1"
                     /translation="MHTAIKKYIITFIFATVIAHSLFAQSCKINYVITDTINNKTLQS
                     FKLLQQFNDSIACINYINQLPNNLQAKGYISASVDSIFIYKNNYTVTIFVGKKFNWKL
                     LKISDTDLISLQLAGIDHTIFSAKPFNPKQVELVQNNLLEYFVNNGYPFASIGFSNLI
                     IEENDITATLRINKGIFYSMDSISIIGNAKISKQFLYKYLNLPKKSLFSLKKLQSIDK
                     KIAELNFINSYHPSQITMLGASYYTNLFLNNKKSNQVDAIIGLLPNNTQNNGSLLLTV
                     DAKLKLENAFAKGETIGVLWQQLQPKTPRLNLLFQYPYIFNSAFIADFNFNLFKKDTS
                     FINIQSSIGFIYKPNYKQHIKFALQINQSNLLDIDTTTLKLTKQLPDIIDVTINNIAI
                     EYGYNSTDYKFNPRRGTEILLLSALGNKKIKKNNSISQLKDGNFNFSNLYNNLKLNSY
                     QIKIQTEASHYFSLSKYKILKTKINAGVIYSPTVYRNEMYQIGGLKLLRGFDEESIFT
                     DKYLVATTEYRYLFDVNAFFNCFIDYALSNNSIFNKSYQYLGLGGGLSFETKQGIFNI
                     AIATGKRNDETINLKRTKIHIGFVSIF"
     gene            4010..5053
                     /locus_tag="PLY81_05560"
     CDS             4010..5053
                     /locus_tag="PLY81_05560"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNF29727.1"
                     /translation="MYKSKIGGIGMYVPTNIVTNKDLEKIMDTSDEWIQERTGIQERR
                     IAHRTNETTTTMGVEAAKIAIDRAGITAQDIDFIVFATLSPDYYFPGCGVLVQRALQM
                     KEIGALDIRNQCSGFIYALSVADQFIKTGMYKNILVIGSEKHSFGLDYTTRGRNVSVI
                     FGDGAGAVVLQQCNNESGILSTHLHSNGNDAETLAMYNPGTHANYWKKDLASFDEATI
                     GEMFMSHSMIDNAQNFPFMDGPAVFKKAVVKMPEVILEALQANNYKPSDIDMLIPHQA
                     NLRIIQFIQHSLKLADDKVYNNIQYYGNTTAASVPIALCEAWEKGKIKTGDLVCLTAF
                     GSGFTWASALIKW"
BASE COUNT         1931 a          758 c          711 g         1719 t
ORIGIN      
        1 attgcaccag caaaatttgc tgcagtaata accaaaatag gtccaattat attaggtaaa
       61 atatgtttaa caataattct aaaatgatta aagcctaaca ctcttgcagc ttcaatatat
      121 tctaattctt ttgcaatcat tacctgacca cgaactaatc tagctacatt tacccacatg
      181 gttaaaccaa ctgcaataaa aacttgccaa aaacctttgc ccaatacaag tgtaatggca
      241 aacactaaaa gtaaagtagg aatactccaa acaatattta ttaatagcat aattacattg
      301 tcggtttttc ctttaaaata acctgcaaca gcacctaaaa aaacgcctat agttaatgaa
      361 ataattgcag caatacaacc tacgcctaaa cttactcgag agcctattat caatctgctt
      421 aaaatatctc ttccaaactt atcggttcct aaataaaatg tttgttttat aatattgttc
      481 tcttcaaaat tgctaccttg tatttttatt actttatttt ctgtaatgtc ttcatcaata
      541 aatttttgat agtataaact atcctttaat attttatatt cggttatggg agtataaaaa
      601 tagttatctg atttgccgtt gagcaaaatt gtaaaaaaat tttcttttgc agattctctc
      661 tgttttgttt ttaataaata aatggaaaag cctggttttt tgctaccaat ttctggaata
      721 attctattgg cattgctgct attttctggt gcaattaaat aggcaaatat tgcaattatt
      781 aatactaaaa aaataaaaag taatgcaaaa actgctgctt tattttttaa aaaccttaag
      841 ctaaaactat tgttgttact ttgcattata gaaactttac tattttacat ttcttccttt
      901 ccaattataa gtaccaaatt ttcctaacca tccacttacc acaatataaa ctatatgtaa
      961 gggttgtaaa aatggaaacc accaaataaa tggctgagaa aaaaactttg caacaggcaa
     1021 tgcaaaagca agttctgcaa tagttttaag cattataaac agcagccaat accataaaaa
     1081 aataaaatta aaaatactta aaaaaggcat tataaataat gtaacattca ataaataaac
     1141 caataccaac actataaaaa tactggtatc ttttgtttta aattgctctg ctttacttgc
     1201 ccatcttatt ctttgattaa aaaaagaact ccaagttggc atagggtgtg tggtaacaat
     1261 tgcttgttta gaaaataaaa agcttactcc attagtgtaa tggtttttaa ttttattcat
     1321 taaaagcata tcatctccgc tggcaatatt atcaattcct ttaaatccat ttacttcaaa
     1381 aaaaacctct tttttatacg ccaaattagc cccattacac atattatgaa agcctgcatt
     1441 tacagatgca gcagttatac cctgtaggct cataaaatct atatattgaa aagtttgcaa
     1501 caaggaacca ttgttagtaa aagcaacagg agctgcaaca aaaacagtat cattttcttg
     1561 aatccatgca tcaaagattg ataaccattt tgtttgcatg aagcagtcag catctgtagt
     1621 tattatccag tttgcttttg cataactaat tgcattctct attgctttct ttttgtaaga
     1681 gttcaacatt tccccatcta gctcatctac taactttatc agtttaagat ttgaatactg
     1741 gtgctgcaat tcttctacaa ttaaagaagt attatcagag ctatggtcat ctatcacaat
     1801 aatttcaaac aaagttgtag ggtactgttg ctgtagtata gatgttaaac aagttcttat
     1861 gttagcttct tcatttctcg caggaattat aatactaaag cttgtagtgg gagtataatt
     1921 ttgggggaga ttaaaatgtt ttaattgtaa gaaccatttt ctataaaaca acaaaacaat
     1981 ggcgtaagcc acaaaacaca aaaaagtaaa aattaaaaac aagttaagcc acatgcatac
     2041 aaagaaacaa tatagtacaa tataatgctt tgtttagcca atattaaatg aaatttaaac
     2101 aaattgcgtc cttcatactc tgtaatattt tagctttaca actgttaata ctgatgcata
     2161 ctgctataaa aaaatatatt atcaccttta tttttgctac agttatagct cattccttat
     2221 ttgcacaaag ctgtaaaata aattatgtta taacagatac aattaacaat aaaactttgc
     2281 agagctttaa acttttgcag cagtttaatg atagcatagc ttgtattaat tatatcaatc
     2341 aattacctaa taacctgcaa gcaaaaggat atattagtgc atcggtagat agtattttta
     2401 tttataaaaa caactataca gtaacaattt ttgttggcaa aaaattcaat tggaaattat
     2461 tgaaaatttc tgatactgat ttaatttctt tacaattagc aggtatagac catactattt
     2521 tttcggctaa accatttaat ccaaagcagg ttgaattagt tcaaaataat ttgttagaat
     2581 attttgtaaa caatggctac ccttttgcca gtataggttt tagtaattta ataatagaag
     2641 aaaatgatat aactgcaact ttaagaataa ataaaggtat attttattca atggatagta
     2701 ttagcataat tggtaatgct aaaataagta agcagttttt atacaaatat ttaaaccttc
     2761 ctaaaaaaag tttatttagt ttaaaaaaat tacaaagtat tgataaaaaa attgcagaat
     2821 taaattttat taattcttat cacccaagcc aaataaccat gcttggagca agttattata
     2881 caaatctgtt tttaaataat aagaaaagta atcaggtaga tgcaattatt ggcttattac
     2941 caaacaatac tcaaaacaat ggttctttac ttttaactgt agatgcaaaa ttaaaattag
     3001 aaaatgcttt tgcaaaaggt gaaactattg gagttttgtg gcaacaatta cagcctaaaa
     3061 cacccaggtt aaatttatta tttcagtatc cttacatatt caattcagca tttattgccg
     3121 attttaattt taatcttttt aaaaaagata cttccttcat aaatatacaa tcttctattg
     3181 ggtttattta taaaccaaat tataagcaac atataaaatt tgctttgcaa ataaatcagt
     3241 ctaatttatt agatatagat acaactactt taaaattaac caagcaattg ccagatataa
     3301 ttgatgtaac tattaacaat attgctattg aatatggtta taatagtaca gattacaaat
     3361 tcaatccacg aagaggaact gaaattttat tattatctgc tttaggcaat aaaaaaataa
     3421 aaaagaataa tagtatatct caattaaaag atggcaattt taatttttct aatttataca
     3481 acaacttaaa attaaacagc tatcaaataa aaatacaaac agaagcatct cattatttta
     3541 gtttgagtaa atacaaaatt ttaaaaacaa aaattaatgc aggagttata tatagcccta
     3601 ccgtttacag aaatgaaatg taccaaattg gtggtttaaa attgttaaga ggctttgatg
     3661 aagaaagtat ttttactgat aaatatttgg ttgccacaac cgaatacaga tatctttttg
     3721 atgtaaatgc attttttaat tgctttattg attatgcttt aagcaacaac tccatcttca
     3781 acaaaagcta tcaatacctt ggcttaggtg gtggcttaag ttttgaaact aaacaaggta
     3841 tttttaatat tgcaatagca acaggcaaaa gaaatgatga aactataaat ttaaaacgaa
     3901 ccaaaataca cattggtttt gtaagtatat tttaatacaa tatcgttcat caagtttcat
     3961 ttttaaatct tcttatttta gtttattttg ccaaaataaa aatagcttta tgtataaaag
     4021 taaaataggt ggaattggta tgtatgtacc aaccaatatt gttaccaata aagatttaga
     4081 aaaaataatg gatacaagcg atgagtggat tcaagaaaga acaggcatac aagaaagaag
     4141 aattgctcat cgtactaatg agacaaccac aactatgggt gtggaggctg ctaaaattgc
     4201 tatagataga gcaggaatta ctgctcaaga tattgacttt attgtttttg caacactaag
     4261 ccccgattat tattttcctg gatgtggtgt attagttcaa agagctttgc aaatgaaaga
     4321 aattggtgca ttagatatac gaaaccaatg cagtggtttt atttatgctt taagtgttgc
     4381 cgaccagttt attaaaacag gtatgtacaa aaacatttta gtaattggca gcgaaaaaca
     4441 cagctttgga ttagattata ccacaagagg aagaaatgtt agtgtaattt ttggtgatgg
     4501 agcaggtgcc gttgtactac aacaatgcaa taatgaaagt ggtattttaa gtacccattt
     4561 acacagcaat ggtaacgatg cagaaacctt agccatgtat aaccctggca cacatgctaa
     4621 ctactggaaa aaagatttag caagttttga tgaagctacg ataggagaaa tgtttatgag
     4681 ccatagtatg atagacaatg ctcaaaattt tccttttatg gatgggccag cagtatttaa
     4741 aaaagccgtt gttaaaatgc cagaagttat cttagaagca ttacaagcaa acaattataa
     4801 accttcagat atagatatgt taattccaca tcaggctaac ttaagaataa tacaatttat
     4861 acaacattct ctaaaactgg ctgatgacaa agtgtataac aacattcaat attatggtaa
     4921 tacaacagct gcaagtgtac caattgcact ctgcgaagct tgggagaagg gtaaaattaa
     4981 aacaggagat ttagtttgct taactgcgtt tggtagtgga tttacttggg caagtgcatt
     5041 aataaagtgg taattttatt gctacaaatt cttaccatat tgtttcactc aataaaaaat
     5101 ttaaaaatga aattcattt
//
LOCUS       DAOQHP010000136         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9459_length_5062_cov_3.486319, whole genome shotgun sequence.
ACCESSION   DAOQHP010000136 DAOQHP010000000
VERSION     DAOQHP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9459_length_5062_cov_3.486319"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..419
                     /locus_tag="PLY81_05565"
     CDS             198..419
                     /locus_tag="PLY81_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HNF29728.1"
                     /translation="MNKLRAFLETSAFGVCSNIGDKLGIASSKIRMWFIYISFATMGS
                     PIIVYMILAFWLNIKKYIYFAKRNPLRYL"
     gene            476..1438
                     /locus_tag="PLY81_05570"
     CDS             476..1438
                     /locus_tag="PLY81_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF29729.1"
                     /translation="MQNFNFTYNNFEELLKQYAKSFNVRIKDKAVILPEQSGKGSICY
                     YQLNNGFQVLVNNFSVFNDITFQHQKTNEEFFILRLDEIVTQEEIDDESKSAAVLANT
                     SFDWIFYVKKGAQINSIHVLFTKEWLNNFLDGEKKVDYIKKYLSLKLATFNYESLDAE
                     YKRLMKEVLLCNDKTPLKLLVLQNRIMLLVERFFTRMILKINDNSFEVKVSDDDLKRL
                     VLVEEELLKDFSIPPPGIPKLARMAAMSPSKLKIIFRDIYGLPIYQYFQKHRMNRAKA
                     MLISQKYTVKEVGFELGYTNLSNFAKAFKKSFDQLPSDLFEVKT"
     gene            complement(1445..2695)
                     /locus_tag="PLY81_05575"
     CDS             complement(1445..2695)
                     /locus_tag="PLY81_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNF29730.1"
                     /translation="MADKILCPNCKHQFDISDVLKEELEKKLRGQAKEWQEKKETEFR
                     QKEQLLQQKFNDWEKQSEKKHVEEMQKLQENLHQTIQKNVAQDFENKLKILQQTVEDN
                     EEKLKQSREKELIFLQKERALKEKEVELEIDIEKRILAEREILKNQIQKEELVKAEIK
                     EESHRLKIKELEKQLDDQKKLADEMKRKAEQGSMQLQGEVQEILLEELLTVNFPFDTI
                     VEVEKGIKGADCVQIVKTQFGFEAGKIIFESKRTKDFNIEWIEKLKKDMRQLGADIAV
                     IVTQTFPKDMDRFGEKDGVYICSISEVKSMITLLRAALIKIYDAKKSQENKGDKMVLL
                     YDYLTGNEFNQQWKAIREGFMAIKSSIAKERDAMEKLWKVREKQLEKVLLNAAHIHGS
                     VEGIAGIEAMNLTLDFEDKNLLEE"
     gene            2798..4909
                     /locus_tag="PLY81_05580"
     CDS             2798..4909
                     /locus_tag="PLY81_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF29731.1"
                     /translation="MIKFFPDTTFDQLEFTKIKNILQGYCTTEYAKNKIADLRVHTRK
                     DFIETALQQTNEFKNLSLQAQYFPNDFSLNLSKEIKLLSIDGATLREEELMHIKLLTN
                     NAANIFRWFDTERKQAYAYLHKIIEGSYYEKNIILLIEAVLDDYGAVKDNASEDLFII
                     RNKLYRKRNELRRMFEKVLQKLSKAGYSADIEESFSNGRRVVAVFSEHKRKVKGILHG
                     ESDTRKTAFIEPEETIELNNEIYLLEGEERKEILKILKTLTVSLKPFAPLIQSYLIIA
                     GEFDFIKAKSKLALSYQGNMPNLTDKAYINIKEAYHPLLYLYNKNNHKKTTPLNLKLD
                     EKNRILIISGPNAGGKTVTMKTIGLLQLMLQSGLLVPVHPTSEMGIFKQIFIHIGDTQ
                     SIEFELSTYSSHLLAMKHFIEMANGKTLFFIDELGSGSDPVLGGAFAEVILEELCKKH
                     AMGIVTTHYLNLKVMANHTKGIINGAMAFDEKNLKPMYVLTIGKPGSSYTFAIAQRIG
                     LSPHLISKAKALVDDNHFKLDNLLNNTEKNLHALYVEKKQLQLLLKSNEKLKKEMEQV
                     INKEKHNQQIELLKNQNKISEERIVYLKEMERKLKQIVLDWKKNEDKNEVIKNLNNLL
                     FKKKEQTVVNKLAKKTNQTFKEIQNVLEVGKLVKLKKNYQVGVLKEIRGKRGIVQIGQ
                     LPINVELTDLVTVEKIDTNYK"
BASE COUNT         1847 a          714 c          702 g         1799 t
ORIGIN      
        1 aaacccttct aatatggcag caaatacaaa taccaaaagc cataaataaa aaattttgat
       61 tggttttaaa aaatactaaa aattattttt acaggcagca aaattacaaa taaatgacct
      121 ttaatataaa tatgttttaa agaatttagt tataatttta tattttgcat attaaataga
      181 tttttagatt aaaaaacatg aataaactta gagcattttt agaaacaagt gcatttggtg
      241 tatgcagtaa cattggtgat aaattaggta ttgcaagttc taaaattaga atgtggttta
      301 tttacatttc atttgctaca atgggctctc caattatagt gtatatgatt ttagcctttt
      361 ggttaaatat taaaaagtat atttattttg ctaaacgaaa cccattaagg tatttataat
      421 gttttaaaaa gttttaattt ttttaatttt aaaatccaaa ttacttcatc tcaaattgca
      481 gaattttaat tttacataca ataattttga agagctttta aagcaatatg ctaaatcttt
      541 taatgttaga ataaaagaca aagctgttat tttgccagaa caatctggaa aaggttccat
      601 ttgttattat caattaaata atggctttca ggttttagtt aataattttt ctgtattcaa
      661 cgatattact tttcagcatc aaaaaactaa cgaagagttt tttattttaa gacttgatga
      721 aattgttact caagaagaaa tagacgatga atctaaaagt gctgctgtat tagctaatac
      781 ttctttcgac tggatatttt atgtaaaaaa aggggcacaa atcaatagta ttcatgtatt
      841 atttactaaa gaatggttga ataatttttt ggatggcgaa aaaaaggttg attatattaa
      901 gaaatatttg agccttaaat tggcaacttt caattacgaa tctttagatg ctgaatataa
      961 gaggttaatg aaagaggttt tactttgcaa tgataaaaca cctttaaaat tactggtttt
     1021 acaaaatagg ataatgcttt tagtagaaag attttttacc agaatgattc ttaaaataaa
     1081 tgataatagt tttgaggtaa aagtatctga tgatgatttg aagaggttgg tattggtaga
     1141 agaagaactg ctgaaagatt tttcaattcc tccaccaggt attcctaaac ttgcaagaat
     1201 ggctgctatg agcccttcta aacttaaaat tatttttaga gatatttacg gattacctat
     1261 ttatcaatat tttcaaaagc acagaatgaa cagagccaag gctatgttaa tatctcaaaa
     1321 atatactgta aaagaagtag gttttgaatt gggttatacc aacttaagca attttgctaa
     1381 agcttttaaa aaatctttcg accaattacc cagtgatttg tttgaagtaa aaacttaatc
     1441 cttattattc ttctagtaaa tttttatcct caaaatctaa ggttaaattc atagcttcaa
     1501 tacccgcaat gccctcaaca gagccatgaa tatgagctgc attgagtaat actttttcta
     1561 attgtttttc tctcactttc caaagttttt ccattgcatc tctttctttt gcaatagagg
     1621 atttaattgc cataaagcct tctcttatag ccttccattg ttggttaaat tcattgcctg
     1681 ttaaataatc gtataataaa accattttat cgcctttatt ttcctgactt tttttagcat
     1741 catatatttt aattaacgca gcccttaata aagttatcat gctcttaact tcagaaatag
     1801 aacatatata aacgccatct ttttcgccaa acctatccat atctttcgga aaagtttgtg
     1861 taacaattac tgcaatatcg gcacctaatt gcctcatatc tttttttaac ttttctatcc
     1921 actctatatt aaaatctttg gttcttttac tctcaaaaat tatttttcca gcttcaaagc
     1981 caaattgtgt ttttacaatt tgtacacagt ctgctccttt aattcctttt tcaacttcaa
     2041 caattgtatc aaatggaaaa tttacagtta ataattcttc taataaaatc tcctgcacct
     2101 ctccttgcaa ctgcatgctt ccttgctctg cttttctttt catctcatct gcaagttttt
     2161 tttgatcatc taattgcttt tctaactctt taatttttaa tctatggctc tcttccttta
     2221 tttcagcttt aactaattct tctttttgaa tttggttttt taatatttct ctttctgcta
     2281 atattctttt ttctatatca atctctagct ctacctcttt ttctttcaat gctctttcct
     2341 tttgcaagaa aattaattct ttttctctcg attgttttag tttttcttca ttatcctcaa
     2401 cagtttgttg aagtatttta agtttattct caaaatcctg agctacattt ttttgtatag
     2461 tttgatgcaa attttcttgc agcttttgca tttcctccac atgttttttt tctgattgct
     2521 tttcccaatc attaaatttt tgttgtaaaa gctgttcttt ctgcctaaat tcagtttctt
     2581 ttttttcttg ccactctttt gcttgccctc ttaatttttt ttctaactct tcttttaata
     2641 catccgaaat atcaaattgg tgtttgcaat tagggcataa tattttatct gccataagaa
     2701 tattttggag gcaaattaac cttttaatag caaattttaa aaatcatatt tagcctttaa
     2761 attcgcagat agaaatacta aataagaaat tatttatatg ataaaatttt ttcctgatac
     2821 aacttttgat caattagaat ttactaaaat taaaaacatt ttacaaggct attgtacaac
     2881 agaatatgct aaaaataaaa ttgcagattt aagagttcat accagaaagg attttattga
     2941 aactgcctta caacaaacca atgaatttaa aaacttaagc ttacaggctc aatattttcc
     3001 taacgacttt tccttaaacc tttcaaaaga aattaaactt ttaagtattg atggtgcaac
     3061 tttaagagaa gaagagctaa tgcacattaa acttttaacc aataatgctg ccaatatttt
     3121 tagatggttt gatactgagc gaaaacaagc ttatgcttat ttgcataaaa ttattgaagg
     3181 gagttattat gaaaaaaata ttatactatt aattgaagct gttttagacg attatggtgc
     3241 agttaaagat aatgccagcg aagatttatt tattataaga aataaattgt accgaaaaag
     3301 aaatgagtta agaagaatgt ttgaaaaagt attacaaaaa ctttcaaaag caggttatag
     3361 tgccgatatt gaggaaagct ttagtaatgg tagaagagta gttgctgttt tttcggaaca
     3421 taaaagaaaa gtaaaaggca ttttacatgg tgaaagtgat acaagaaaaa ctgcatttat
     3481 tgagccagaa gaaaccattg aacttaataa tgaaatttac cttttagaag gcgaagagag
     3541 aaaagaaatt cttaaaattc ttaaaacact aaccgttagt ttaaaaccat ttgccccatt
     3601 aattcaaagc tatttaatta ttgcaggcga atttgatttt attaaagcaa aaagcaaatt
     3661 agctttaagc tatcagggta atatgcccaa ccttacagat aaagcatata ttaatattaa
     3721 agaggcttac caccctttac tgtatttgta caataaaaac aaccataaaa aaacaactcc
     3781 acttaattta aaactagatg aaaaaaacag aatattaatt attagtgggc caaatgcagg
     3841 aggcaaaact gtaacaatga aaaccattgg gctactacaa ctaatgctgc aaagtggttt
     3901 attagtacca gtgcatccta catctgaaat gggtattttt aagcaaatat ttattcatat
     3961 tggcgataca caaagtatag aatttgagtt gagtacttac tctagtcatt tattggcaat
     4021 gaagcatttt attgaaatgg ctaatggtaa aacattgttc tttattgatg aattaggcag
     4081 tggtagcgac ccagttttag gtggtgcatt tgcagaagtt attttagaag aattatgcaa
     4141 gaaacacgct atgggtattg ttacaacaca ttacttaaac ttaaaagtaa tggccaacca
     4201 tacaaaagga ataataaatg gtgctatggc ttttgacgaa aaaaatttaa aacctatgta
     4261 tgtattaacc ataggtaaac caggtagtag ttacacattt gccattgcac aacgtattgg
     4321 gttatcgcca catttaattt ctaaagcaaa agctttggta gatgataatc attttaaatt
     4381 agacaacctt ttaaataaca ctgaaaaaaa ccttcatgca ttgtatgttg aaaagaaaca
     4441 attacagctt ttgcttaaaa gcaacgaaaa attaaaaaaa gaaatggagc aggttataaa
     4501 taaagaaaaa cataatcagc aaattgaatt attgaaaaat caaaataaaa ttagcgaaga
     4561 aagaattgtt tacttaaagg aaatggaaag aaaattaaag caaattgttt tagactggaa
     4621 aaaaaatgag gataaaaacg aagttataaa aaatttaaac aatttactct tcaaaaagaa
     4681 agaacaaaca gtggtgaata aacttgctaa aaaaacaaat caaacattta aagaaataca
     4741 aaatgtttta gaagttggta aactggtaaa actaaaaaag aattatcaag taggggtttt
     4801 gaaggaaata agaggaaaaa gaggtatagt gcaaattggg cagctaccta taaatgtaga
     4861 actaacagat ttagttacag ttgaaaaaat agataccaat tataaataaa tttttaaaaa
     4921 aaatataatc cacatatttt tatctctaat ttttacgtta caataacctt gtaaaaaaaa
     4981 atgcccataa ttgcaaacta aatgaaccaa actggtactg gtataagtaa attttcttta
     5041 gaaagccttg tagcagcttt tt
//
LOCUS       DAOQHP010000137         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9471_length_5058_cov_4.401959, whole genome shotgun sequence.
ACCESSION   DAOQHP010000137 DAOQHP010000000
VERSION     DAOQHP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9471_length_5058_cov_4.401959"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..683
                     /gene="rpe"
                     /locus_tag="PLY81_05585"
     CDS             36..683
                     /gene="rpe"
                     /locus_tag="PLY81_05585"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNF29732.1"
                     /translation="MPIIAPSLLSANFLQLEKDCNMLNESQADWFHLDVMDGRFVPNI
                     SFGPMIVEFISKATTKVCDVHLMIEEPEKYAEQFKNAGANILTVHLEACKHLHRNIQQ
                     IKSLGMKAGVAINPHTPVSLLEDILYDIDLVCLMSVNPGFGGQKFIPQTIEKIKQLKT
                     MCVQKNLKTYIEIDGGVTLDNADSIIKAGANVLVAGNTVFKASNPINAIAELKSK"
     gene            750..823
                     /locus_tag="PLY81_05590"
     tRNA            750..823
                     /locus_tag="PLY81_05590"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Asp,seq:gtc)
     gene            complement(1094..2500)
                     /gene="lpdA"
                     /locus_tag="PLY81_05595"
     CDS             complement(1094..2500)
                     /gene="lpdA"
                     /locus_tag="PLY81_05595"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF29733.1"
                     /translation="MTYDVVVIGSGPGGYPAAIRASQLGFKVAIIEKENLGGICLNWG
                     CIPTKALLKSAQVNEYLKHSADYGINATGSADFNAVIKRSRGVADKMSKGVQFLMKKN
                     KIDVIMGYGKVKGKGQVEVTASDNTIQIIACKYIVIATGGRSRELPNLKQDGSKVIGY
                     REAMVLANQPKSMIIVGSGAIGVEFAYVYNSMGTKVTIVEFMPRIVPVEDEDISKELE
                     KQYKKQGIEIMTNSSVESVDTTGAGVKALVKKQDGSTLTLEADVLLSAVGVVANIENI
                     GLEETGIKTEKGKVIVDKYQQTSLLGVYAIGDCTPGQALAHVASKEGINAAEHMAYLE
                     KKHHHLPDPMDYNNIPGCTYCTPEISSVGYTEKAAKEAGYEIKVGKFPFVASGKAVAG
                     GATEGFVKVIFDAKYGEFLGCHMIGANVTDMIAEAVVARKLETTAHEILNAVHPHPTM
                     SEGLKEAVAAAYGEAIDI"
     gene            complement(2582..3322)
                     /gene="recO"
                     /locus_tag="PLY81_05600"
     CDS             complement(2582..3322)
                     /gene="recO"
                     /locus_tag="PLY81_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNF29734.1"
                     /translation="MQIHSTKGIVIRAVKYGDTSIIATIYTQLFGLQSYIIKGVRGSS
                     AKSTYKANCFQPAAILELEVYHHPQKQIQFIKNYSWSYVYKNIFSNVICNSVAIYIIE
                     LLQHSIKQPEPNTDLYNFIEETLIITDTETKDTISNLPIFFTLLLCNHLGFKINGCYL
                     EITPVLDFQEGTFVAETPTHNYFAVEWEAETISWLNSINNYQDLKLLQLNRINRKIIL
                     QHLLQYFTIHIHNFGSLKSYQILQEVLD"
     gene            complement(3331..3972)
                     /locus_tag="PLY81_05605"
     CDS             complement(3331..3972)
                     /locus_tag="PLY81_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF29735.1"
                     /translation="MDTPKINIAIVDDHQIVIDGIVALLTGYHSINIVATSTSGLVML
                     EILQSKKVQILLTDIMMPAISGQDLAKQVKANFPEIKILALSMSGQGHIVSKMIEDAD
                     IAGYVLKNIGKDELFHAIENIANGGVYFSQEVLNELQHFTSLKKENETINLTVREIEI
                     IKLIEKEFSNKEIANELFISERTVETHRKNIFRKTGTSGIVGLLKYAYEHKLI"
     gene            complement(4029..>5058)
                     /locus_tag="PLY81_05610"
     CDS             complement(4029..>5058)
                     /locus_tag="PLY81_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF29736.1"
                     /translation="KEKQIQDQQITINKNLYELSKQEVELYQNKLNIAASNIQLFNQN
                     ELILTQRLDSTNKEKQIALLTKEKKIDKLQIKNNELESNRKTTILIVSIILTILTVLI
                     AFSGYKRYKLKKEKQLQHEIFLQKQINTQNILQAEENERKRIASDLHDGIGQLMSAAK
                     MNLSAMETTIPFVSNEQKSSYQNALNLVDESCKEIRAVSHNMMPNALLKAGLDNALKA
                     FIYQMSNSGLEINLYTEGLETRLSANTETFLYRIIQECINNVIKHAKATKLDISLIKD
                     EDGISAMVEDNGKGFEVKNINNSEGIGLKNIKSRVEYLSGTVEWKSASEKGTLIAIHI
                     PIEAKQIA"
BASE COUNT         1542 a          885 c          724 g         1907 t
ORIGIN      
        1 gtattatcta tttattaaat ttttaaaatt taattatgcc aattatagcc ccatctttat
       61 taagtgctaa ttttttgcag ctagaaaaag attgcaatat gctaaacgaa agccaggcag
      121 attggtttca tttagatgta atggacggca ggtttgtacc taatatttct tttggaccaa
      181 tgatagttga gtttataagt aaagccacca caaaagtttg tgatgtacac ttaatgatag
      241 aagaaccaga aaaatatgca gagcaattta aaaatgctgg agccaatatt ttaactgtac
      301 atttagaggc ttgtaaacat ttacaccgaa atattcaaca aattaaaagc cttggtatga
      361 aagcaggtgt agcaataaac ccacacacgc ctgtaagctt attagaagat attttatacg
      421 atattgattt ggtttgttta atgagtgtaa acccaggttt tggggggcaa aagtttatac
      481 cacaaacaat agaaaaaatt aaacaactta aaaccatgtg tgtacaaaag aacttaaaaa
      541 catatattga aatagatggt ggcgttacat tagacaatgc agattctatt attaaagctg
      601 gggccaatgt tttagtagca ggaaatactg tttttaaagc atctaacccc ataaatgcaa
      661 tagctgaatt aaaaagtaaa taatttttaa aaaagatttg ctaaagaaaa aatcttgccc
      721 ttatatttgc aaccccgaag ggggaaattg gatcggtagt tcagttggtt agaatgccgc
      781 cctgtcacgg cggaggtcgc gggttcgagt cccgtccggt ccgcaaaaaa ggttgttatt
      841 aatagcaacc ttttttgatt tagttttatc ctaaactgcc tgtttcaaaa actaataagg
      901 gtggttcatt ctccagctat ttttagtatt accgatagta ttatttaagt attcctatct
      961 ttttatttca aatttatgcc acatatcagc ctgtattttt gtttgtaatg gaatgaatgc
     1021 aataataaat tgctataaaa aacaaaggct atctaaaact agatagcctt ttgaaaaaat
     1081 atatattata caattaaatg tctatagcct cgccataagc agctgcaaca gcttctttta
     1141 atccttcgct cattgttggg tgcggatgta cagcatttaa aatttcgtgt gctgtagttt
     1201 ctaattttct ggcaacaaca gcttcggcaa tcatatctgt tacgtttgca cctatcatgt
     1261 ggcagcccaa aaattcacca tattttgcat caaaaataac ttttacaaat ccttcagttg
     1321 caccaccagc aactgctttt ccactagcaa caaatggaaa cttaccaacc ttaatttcgt
     1381 aacctgcctc ttttgctgct ttttcggtat agccaacact acttatttct ggagtacaat
     1441 aggtacatcc tggaatattg ttgtaatcca tagggtctgg taagtgatgg tgttttttct
     1501 ctaaataagc catgtgttct gctgcattta ttccttcttt agatgccaca tgtgctaaag
     1561 cttgaccagg tgtacaatct ccaattgcat atacacctaa taatgatgtt tgctgatact
     1621 tatcaactat aacttttcct ttttctgttt taattcctgt ttcttctaag ccaatatttt
     1681 cgatgtttgc aactacccca actgcactta ataacacatc tgcctctaag gtaagtgtac
     1741 tgccatcttg ctttttaacc agggctttta cgccagctcc ggtagtatct acactttcaa
     1801 cagatgagtt ggtcataatt tctatacctt gttttttgta ttgcttttct aattctttag
     1861 atatgtcttc atcttctaca ggcacaatac gtggcataaa ctcaacaatg gtaactttag
     1921 tacccatgct attatataca taagcaaact ctactccaat agcaccacta ccaacaataa
     1981 tcatgctttt gggctgattg gctaaaacca ttgcttctct gtatccaata accttactac
     2041 catcttgttt taaatttggt agctctctac ttctgccacc tgttgcaata acaatgtatt
     2101 tacaagcaat tatttgtata gtattatccg atgcggttac ttctacttgt ccttttcctt
     2161 tcacttttcc ataacccata attacatcaa tcttattctt cttcattaaa aactgtactc
     2221 ccttactcat tttatctgca acacctctgc ttcttttaat tacagcatta aaatcggctg
     2281 aacctgttgc attgatgcca taatctgcac tatgttttaa atattcattt acttgagcac
     2341 tttttaatag tgcttttgtt ggaatacaac cccaatttaa acaaatgcca cctaagtttt
     2401 ctttttcaat aatggctact ttaaaaccta attgagatgc tctgatagct gcaggataac
     2461 cacctggtcc gctaccaatt actactacat cgtatgtcat agctttttaa ttttaaaatt
     2521 gtggttgcaa aaatacttgc aaaagccgaa atggaaaaat gagattattg tatggcttgg
     2581 tttaatctaa tacctcttgt aaaatttgat agctttttaa gctgccgaaa ttatgtatat
     2641 gtattgtaaa gtattgaagt aagtgttgta aaataatttt tctattaatt ctatttaatt
     2701 gcaaaagttt taaatcttga taattattga tgctgtttaa ccaactgata gtttctgcct
     2761 cccactcaac tgcaaaatag ttatgagttg gcgtttcggc aacaaaagtt ccttcttgaa
     2821 aatctaaaac tggtgttatt tctaaataac aaccatttat tttgaatcct aaatgattac
     2881 ataacagaag ggtaaaaaat ataggtaaat tacttatagt gtctttcgtt tcagtatcgg
     2941 taattattaa tgtttcttca ataaaattat ataaatctgt attaggttcg ggttgtttta
     3001 tactatgttg taatagttca ataatataaa tagcaacact attacaaatt acattagaga
     3061 aaatattttt atacacataa ctccaactat aattttttat aaattgtatt tgtttttgag
     3121 ggtgatgata cacttctaac tctaaaattg cagctggctg aaaacaattt gccttgtaag
     3181 ttgatttagc tgatgagcct cttacccctt ttatgatata actctgtaag ccaaaaagct
     3241 gtgtgtaaat ggttgctata atgcttgtat caccatattt tactgctctt attacaatgc
     3301 ctttggtaga gtgaatttgc atgaaagaaa tcagataagt ttatgttcgt atgcatattt
     3361 tagtaaacca acaataccac ttgtaccggt ttttctaaaa atattttttc tatgcgtttc
     3421 aacagttctt tcactaatga ataattcatt ggcaatttct ttattggaga actctttttc
     3481 aattagttta ataatttcta tttctcttac agttaaatta atcgtctcat tttctttctt
     3541 caaactagta aaatgctgta attcatttaa tacttcttgg ctaaaataca ccccaccatt
     3601 tgcaatattt tcaatagcat gaaatagttc atcctttcct atattcttta acacataccc
     3661 tgcaatatca gcatcttcaa tcattttact tacaatgtgc ccctggccac tcatgcttaa
     3721 tgcaaggatt ttaatttcag gaaaatttgc tttaacctgc ttagctaaat cttgaccact
     3781 tattgcaggc atcattatat ctgtaagaag tatttgaact tttttagatt gtaaaatttc
     3841 tagcatcact aacccagagg tagaagttgc cacaatattt atagaatgat agcctgttaa
     3901 caaagcaaca ataccatcaa ttactatttg gtggtcatct acaattgcaa tatttatttt
     3961 tggggtatcc attaggggta ttctacaaag taaagtagtt tttattacta gaaaaatatg
     4021 ttttacaatt aagcaatttg tttagcttct attggaatat gaatggctat taaggttcct
     4081 ttttctgagg cagatttcca ttcaacagtt ccgcttaaat actctaccct cgatttgata
     4141 tttttcaatc ctataccttc cgaattgttg atatttttta cttcaaatcc ttttccattg
     4201 tcttccacca ttgctgaaat cccatcttca tcttttatta aagaaatatc taacttggtt
     4261 gctttagcgt gttttattac attgtttata cactcttgaa ttattctata caaaaaagtt
     4321 tcggtattgg cagataacct tgtttctaac ccttcggtat ataaattaat ttctaaccca
     4381 ctattactca tttggtaaat aaaagctttt aaggcattat ctaaacctgc ttttagtaat
     4441 gcattgggca tcatgttatg gcttactgct ctaatttctt tacaactttc atctaccaag
     4501 tttaaagcat tttggtagct acttttctgc tcattgctta caaatggaat agtagtttcc
     4561 atagcactta agttcatttt agcagcactc attaattgtc caataccatc atgcaaatcg
     4621 cttgctattc ttttacgttc attctcttcg gcctgtaaaa tattttgtgt attaatttgt
     4681 ttttgcaaaa atatttcatg ttgtaattgt ttctcttttt taagtttata ccttttataa
     4741 cctgagaatg caattagcac agttagtatt gttaatatta tagatacaat aagtattgta
     4801 gtctttctgt tgctttctaa ttcattattt ttaatttgta acttatcaat tttcttttct
     4861 tttgttaaaa gggctatttg cttttcttta ttggttgaat ctaacctttg agttagaatt
     4921 aattcatttt gattgaacaa ctgaatatta gaagctgcaa tatttaattt attttgatac
     4981 aactctactt cttgttttga taactcgtac aagtttttat tgatagttat ttgctggtct
     5041 tgaatttgtt tttctttt
//
LOCUS       DAOQHP010000138         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9522_length_5044_cov_3.252756, whole genome shotgun sequence.
ACCESSION   DAOQHP010000138 DAOQHP010000000
VERSION     DAOQHP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9522_length_5044_cov_3.252756"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..555
                     /locus_tag="PLY81_05615"
     CDS             40..555
                     /locus_tag="PLY81_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNF29737.1"
                     /translation="MNLIQILFWFLTGLALFSALMVILSKNPVHSVLWLIVVFFAISG
                     HYILLNAQFLAIVNLIVYAGAIMVLFLFVIMLLNLNKETEPQKNVWMKLAGTIAGGCF
                     LLSVCNVIYNAQVTTLELNKKTVLMGEGNIGLIENLGKKLFTDYVIPFEISSVLFLSA
                     MVGAVVIAKKD"
     gene            574..909
                     /gene="nuoK"
                     /locus_tag="PLY81_05620"
     CDS             574..909
                     /gene="nuoK"
                     /locus_tag="PLY81_05620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNF29738.1"
                     /translation="MPVQYYIYLALALFSIGIIGVLTRRNTIIVFMCIELMLNAVNLL
                     LVAFSKMHHHAAYLQNKASVVGSEGQLFVFFIMVVAAAEVSVGLAIIVMLYRNTHSVD
                     INFLNRLKN"
     gene            1018..2931
                     /gene="nuoL"
                     /locus_tag="PLY81_05625"
     CDS             1018..2931
                     /gene="nuoL"
                     /locus_tag="PLY81_05625"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF29739.1"
                     /translation="MSKLIYLVPLLPFIGFLINGLCRKYLTKPIIATIGSGSIFVSFI
                     ISVVLFFDVKATGLKIFKAFNFINVGAFKIDFAFQLDQLSALFLLIITGIGFLIHLYS
                     SAYMKDEEPPHYARYFAYLNLFVFSMLLLVLGANFLIMFIGWEGVGLCSYLLIGFWFK
                     NDKYNYAAKKAFVMNRIGDVGFLLAIFWLLAKVGTLSYAEVFSPSILSQLSQTDITVI
                     TILLFIGAMGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIARSNVLYSLS
                     EVTQHLVAIIGLATALFSATIAIKQNDIKKVLAYSTVSQLGYMFLGLGVGAYTGAVFH
                     VMTHAFFKALLFLGAGSVIHAMHHEQDIRNMGGLRKKLPITHITFLLACIAISGIPPF
                     SGFFSKDEILAAAYAHSPIYWGIGVATAALTAFYMFRLYATTFLGKFRGTQEQEHHLH
                     ESPSAMTIPLIILAVFSVVAGFVGVPAVLGGNHQLHHYLSTVLVSEKTHTIAHSTEYM
                     LMGISVAIAVIAIVFAISKYSKNPELKEPVGLGKTLANKWYVDELYDSIIVKPLLSLA
                     SFFKNIIEKLFIDGIVNNVGTFVQYASRQFRLVQSGQVANYIMIMVLAIVVFILLWFF
                     DSSILKLAGKLFY"
     gene            2948..4375
                     /locus_tag="PLY81_05630"
     CDS             2948..4375
                     /locus_tag="PLY81_05630"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF29740.1"
                     /translation="MISVLLILIPLVAGLISFFIKSNNAIKQWSLFASLAVLGVVLWG
                     FTCNNNCLNIDTAWLATIGSRFTTKLDGMALSLCLLSAIVYPLIFVSTYKNEYHHANN
                     FYGLMLLALAGLMGVFVAADALLFYFFWELTLIPMYFLCSRWGGEKRIAVTFKFFVYT
                     FLGSLLMLIGILYLYYKTPNHSFALTDFYALHLSVKEQGFIFWLFFIAFAIKMPIFPF
                     HTWQPDTYEQSTTAVTMVLSAVLVKMGVFAIIRWMLPVFPLAVSSNATIVIVLSVIGM
                     LYASLLAIKQDNIKKMIAYSSIVHIGLMCAAIFANNEIALKGVILQMFNHGINILGLW
                     IVADAIEQKTGTIQFSQLGGLAQKQPVLAILFMIMALANVALPLTNAFIGEFLMFSGL
                     FQYNMYASGIALLSIILAAVYTLNMMQKVFYGNTLQATENTTEISTNVQFALIVLAIG
                     IIVLGVYPQPLIDLSKDTVMFILGK"
     gene            4400..>5044
                     /locus_tag="PLY81_05635"
     CDS             4400..>5044
                     /locus_tag="PLY81_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNF29741.1"
                     /translation="MNAIIISALMGVLLMFASMATSNKQIIKNVAIVALLFLLCSNIA
                     DTYSWCTIKVNTRDMFVFTKFGLYFNSLAIAATLIYVLLSGNEIIKTGNYAAEYFTLI
                     FFVLCGISILSSFNNLLMMFLGIEIMSIPLYILTGSAKKNLKSNEAALKYFLMGSFST
                     GILLMGIALVYGASGTFDIHQVAPIGANGLQHAPLLEVIGLLLIFSAMLFKVSAA"
BASE COUNT         1580 a          650 c          892 g         1922 t
ORIGIN      
        1 agatttagaa aaagcaaaag gaaaatagaa ataaacacca tgaacttaat acaaatatta
       61 ttttggtttt taacaggctt agcattgttt agtgctttaa tggttatttt aagcaaaaat
      121 ccagtacaca gtgtactatg gctaatagtt gtattctttg ctatctcagg tcattatatt
      181 ttattaaatg cacagttttt agcaattgta aacttaatag tttatgcagg agccataatg
      241 gttttgtttt tatttgttat tatgctactt aacttaaata aagaaacaga gcctcaaaaa
      301 aatgtttgga tgaaattagc tggcaccatt gcaggtggct gttttttatt gagtgtttgt
      361 aatgttattt ataatgcaca agtaaccaca ttagagctaa ataaaaaaac cgttttaatg
      421 ggtgaaggaa atattgggtt aattgaaaat ttaggaaaaa aattatttac cgattatgta
      481 ataccatttg aaattagtag cgtactattt ttaagtgcta tggttggtgc ggtagtaatt
      541 gcaaaaaaag attaagggtt aaaaaaataa attatgcctg tacaatatta tatatactta
      601 gcattggctt tatttagcat aggaataata ggagtgctta ccagaagaaa cactattatt
      661 gtttttatgt gtattgaatt aatgttaaat gcagtaaacc tattgttggt tgcattttca
      721 aaaatgcatc atcatgctgc ctatttacaa aataaagctt ctgttgttgg tagcgaagga
      781 cagttgtttg tattttttat aatggtggtt gcagctgcag aagtaagtgt aggcttggcc
      841 ataattgtaa tgttgtatag aaatacacac tcggttgaca ttaatttttt aaacaggtta
      901 aagaattaga aaagcatcat aatattttga tgcgattaaa ataaataact taatttttat
      961 tatagcatta gaaaaatgtt ataataaaac ctcgtaaaga ggataatagg aaactgtatg
     1021 agtaaactga tttatttagt accactgtta ccatttatag gatttttaat aaatgggcta
     1081 tgcagaaaat atttaactaa accaattatt gccaccattg gtagtggtag catttttgta
     1141 tcatttatta ttagtgtagt tttatttttt gatgtaaaag ctacagggct taaaatattt
     1201 aaagcattta attttattaa tgtaggagca tttaaaattg attttgcttt tcagttagat
     1261 cagctatctg ctttatttct tttaattatt acaggtattg gttttttaat acatttatat
     1321 tcctctgcat acatgaagga tgaagaaccg ccacattatg caagatattt tgcttactta
     1381 aacctctttg ttttttcaat gttattattg gttttaggag caaacttttt aattatgttt
     1441 attggttggg agggtgtagg cttatgttct tatttactaa ttggtttttg gtttaaaaac
     1501 gataaatata attatgctgc taaaaaagct tttgtaatga accgcattgg tgatgtaggt
     1561 tttttattag ctatattttg gttacttgca aaagtgggta cattatctta tgccgaagtt
     1621 ttttctcctt caatacttag ccaactttca caaacagaca ttacggttat aaccatttta
     1681 ttatttattg gagcaatggg taaaagtgcc caaatacctt tatatacctg gttgccagat
     1741 gctatggctg ggccaactcc tgttagtgca ttaatacatg ctgcaacaat ggtaactgct
     1801 ggtatttata tgattgcaag aagtaatgtg ctatatagtt tgagtgaggt tacccaacat
     1861 cttgttgcaa ttattggttt agccacagca ttgtttagtg caactatagc cataaaacaa
     1921 aatgatatta aaaaagtgtt agcttactct acagttagtc agttaggata tatgtttttg
     1981 gggttaggtg taggtgctta cacaggtgct gtttttcatg taatgacaca tgcatttttt
     2041 aaagccttat tgtttttagg ggcaggctct gtaatacatg ctatgcacca cgaacaagat
     2101 attagaaata tgggaggatt aagaaaaaaa cttcctataa cgcatatcac ttttttatta
     2161 gcttgtattg ctatatccgg tattccacca tttagtgggt tcttctctaa agatgaaatt
     2221 ttagctgcag cttatgctca tagcccaatt tattggggta ttggtgttgc tacagcagca
     2281 ttaactgcat tttatatgtt ccgtttatat gctaccacat ttttgggtaa atttagaggt
     2341 acacaggagc aggaacatca tttgcatgaa agcccaagtg ccatgaccat tccattaatt
     2401 attttagccg tttttagtgt tgttgctggc tttgttggtg tacctgctgt tttaggtggc
     2461 aaccatcaat tacaccacta tttatcaact gttttagttt cagaaaaaac acatactata
     2521 gctcacagta cagaatatat gttaatggga atttctgtag ccattgcagt aattgctata
     2581 gtttttgcaa ttagcaaata cagtaaaaat cctgaattaa aagaaccagt tggtttagga
     2641 aaaacattgg ctaataaatg gtatgtagat gaactttacg attcaattat tgtaaaacca
     2701 ttattgtcat tagcttcctt ctttaaaaat ataattgaaa aattatttat tgatggtata
     2761 gtaaataatg ttggaacatt tgtacaatat gcttctagac aatttagatt agttcaaagt
     2821 gggcaggtag ccaattatat tatgataatg gttttagcaa ttgttgtttt tatattatta
     2881 tggttctttg attcatcaat tttaaaatta gctggtaaat tattttatta agcaatagaa
     2941 aataattatg atttctgtat tacttatatt aattccttta gttgcaggct taatatcttt
     3001 ttttataaag agtaataatg caattaagca atggtcatta tttgcttctt tagcggtatt
     3061 gggtgttgta ttatggggct ttacttgcaa taataattgt ttaaatattg atactgcttg
     3121 gctagcaact ataggtagta gatttacaac caagttagat ggtatggctt tatcactatg
     3181 tttattatcg gcaatagtat atcctttaat ttttgtttca acctataaaa acgagtatca
     3241 tcatgccaat aatttttatg gcttaatgct gcttgcgtta gctggtttaa tgggggtatt
     3301 tgttgcggca gatgcattgt tgttttactt tttttgggaa cttactttaa tacctatgta
     3361 ttttttatgt agccgttggg gaggagaaaa aagaattgca gttacgttta aattttttgt
     3421 atatactttc ttaggttctt tattaatgct tataggtatt ttatatttat attacaaaac
     3481 acctaatcat agttttgcac taaccgactt ttatgctcta catctttcag ttaaagagca
     3541 gggctttatt ttttggttgt tttttattgc atttgcaatt aaaatgccca tctttccttt
     3601 tcatacatgg cagccagata cctacgagca atccacaact gctgttacaa tggtattaag
     3661 tgctgtatta gttaaaatgg gtgtttttgc aattattcgt tggatgcttc ctgtgtttcc
     3721 attagctgtt tcgtccaatg caacaatagt aatagtgtta tctgtaattg gtatgctcta
     3781 tgcttctcta ttagcaataa agcaggataa tattaaaaaa atgattgcct attcatcaat
     3841 agtgcatatt ggcttaatgt gtgctgccat ttttgcaaat aatgaaattg cattgaaggg
     3901 ggtaattcta caaatgttta atcacggaat taatatttta ggactatgga ttgttgcaga
     3961 tgctattgaa caaaaaacag gcaccattca atttagtcaa ttaggaggtt tagctcaaaa
     4021 acaaccagta cttgctatat tatttatgat aatggcatta gcaaatgttg cattgccttt
     4081 aaccaatgct tttattggag agtttttgat gtttagtggt ttatttcaat acaatatgta
     4141 tgcatctggt attgcacttt taagtattat tttggcagca gtttatactt taaatatgat
     4201 gcaaaaagtt ttttatggta acactttgca agctactgaa aatacaactg aaataagcac
     4261 taatgtgcag tttgctttaa ttgtattggc aattggtatt attgtattag gggtatatcc
     4321 tcaaccattg atagatttat caaaggatac agtaatgttt attttaggaa aataattttt
     4381 taaagagatt aggttaatta tgaatgcaat tattatttcg gctttaatgg gtgtgttgct
     4441 aatgtttgca agcatggcaa catcaaacaa acaaattata aaaaatgtag caattgttgc
     4501 attactattt ttattgtgta gcaatattgc cgatacatat agctggtgta ccattaaagt
     4561 aaatacaaga gatatgtttg tgtttacaaa gtttggtttg tattttaact ctcttgcaat
     4621 agctgcaact ttaatatatg ttttactaag tggcaatgaa ataataaaaa caggcaacta
     4681 tgccgcagaa tattttaccc ttatattttt tgtattatgc ggtatctcca tattatcatc
     4741 attcaataat ttattaatga tgtttttagg aattgaaatc atgtcaatac cattatatat
     4801 tttaacaggc tcggcaaaga agaatttaaa gagcaatgag gcagctttaa aatatttttt
     4861 aatgggctct ttttctactg gcattttatt aatgggtata gcattggtat atggggcaag
     4921 tggtactttt gatattcatc aggttgcacc aattggtgct aatggcttac agcatgctcc
     4981 tttgttagaa gttatagggt tattattaat tttttcagcc atgctattta aagtgtcagc
     5041 tgct
//
LOCUS       DAOQHP010000139         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9526_length_5043_cov_3.492181, whole genome shotgun sequence.
ACCESSION   DAOQHP010000139 DAOQHP010000000
VERSION     DAOQHP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9526_length_5043_cov_3.492181"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLY81_05640"
     CDS             <1..827
                     /locus_tag="PLY81_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNF29742.1"
                     /translation="LIKPKISKEEYIKCIHQLKQHIQRGDCYEINFCQEFFAKDVAIN
                     TIEVYKKLTTLSATPFACYYKLNEQYLLCASPERFVCKNGNKIISQPIKGTIKRNLKD
                     SLQDDVLKKQLQNSSKDKTENVMIVDLVRNDLSKICTKGSVIVEELFGVYSFPQVHQM
                     ISTVSGNVQKNINFSEVLQATFPMGSMTGAPKKRVLELTEQYEKSNRGIYSGTVGYIT
                     PENNFDFNVVIRSIMYNAVNKYLSYMVGGGITINSNAEDEYQECLLKATAIKEVLT"
     gene            1008..2021
                     /locus_tag="PLY81_05645"
     CDS             1008..2021
                     /locus_tag="PLY81_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNF29743.1"
                     /translation="MFTIQQELEKRILIIDGAMGTMIQRYKLQEEDYRGTRFINWHKD
                     VKGNNDLLSITKPAVITAIHKEYLLAGADIIETNTFSSTSIAQADYDMQEFAYELNVA
                     SATCAKNAINEYIKETNTTTPKFIAGAIGPLNKTLSLSPDVNNPGFRAVTFDEVVAAY
                     TEQIKGLIDGGVDILLIETIFDTLNAKAAIFAAKKFFRENNIEKPIMISGTITDASGR
                     TLSGQTLEAFYISVKHANPLSIGLNCALGANEMRGHIEELSNIASCYVSAYPNAGLPN
                     AMGEYDEAPEETAHFIEDWAKNKWVNIVGGCCGTTPEHIKHIAENVKLLQPRSLPVLE
                     TTI"
     gene            complement(2063..2692)
                     /locus_tag="PLY81_05650"
     CDS             complement(2063..2692)
                     /locus_tag="PLY81_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNF29744.1"
                     /translation="MQSYLKLLLENKSWAKEKVEVDPDFFTNLAKLQTPEFLWIGCSD
                     SRVSADQITGTQPGEIFVHRNIANLVVNTDVNLMAVLDYAINHLKVKHVIVCGHYGCG
                     GVKASMCKTDLKYILNMWLRNIKDIQRIYEEELNNIADEEKRYDRLVELNVKEQVLNL
                     AKSSIIQNAWKGGDIPHLHGWVYSLNDGIIKPVFEMKAGTKINSIYEFE"
     gene            2861..3295
                     /locus_tag="PLY81_05655"
     CDS             2861..3295
                     /locus_tag="PLY81_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNF29745.1"
                     /translation="MKLTLDTNIVTDDFFNETILLGITTTIKNYKLCLLLNTFMGFHF
                     KLNNNIEICISKRKRKYYFSVYESVLKNSTLVHYLYHNHNDGEYLLPEFKHIDFLWLM
                     KGDILDTTQVEEIKTAIKTLNGVQMVAELTNEQIKTKGNMIF"
     gene            3304..3540
                     /locus_tag="PLY81_05660"
     CDS             3304..3540
                     /locus_tag="PLY81_05660"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNF29746.1"
                     /translation="MWQEIENKLYKKFSFKNFSEAFAFITQVALEAEKMNHHPNWSNS
                     WNIVEIWLYTHDMGNKVTEKDILLSKKIDAIISK"
     gene            3595..>5043
                     /locus_tag="PLY81_05665"
     CDS             3595..>5043
                     /locus_tag="PLY81_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29747.1"
                     /translation="MQEPNKKKVFDYSLLVRIYSYVKPYKHWFYFSLVLAILIAILAP
                     IRPYLIQLTIDKATGKNIIIPTWLQFIFPSKNLNIAAQLVIWITIFQIVFIIFETIIR
                     FVFSYLTAKLGQHIVKDLRVSVFKKILHLRLRQFDKTPIGTLTTRTINDIESINDIFS
                     DGLIPIISDLLTIIFTVATMFWINWQLALIALIPFPFIIIATYYFKESVNASFIRVRN
                     AVANLNAFVQEHLTGINIVQAFAAEEKEFKKFEAINQEHRSANIKAIFAYSVFFPVVE
                     LVLAVSIGLTVWFIAGKQLNPGLLIAFILYLNQIFKPLRIIADKFNVLQMGMIAAERV
                     FKVLDNKDVEPTNNTSANILQLKGDITFNNVWFAYKPQQYVLKNISFNVQQGETVALI
                     GHTGSGKTSIISILNKLYDIEKGEILIDDFNIDKINISTLRKNIAVVLQDVFLFAGTI
                     KDNITLENKLITNEKVIEAAKLIGVHDFIMKLP"
BASE COUNT         1939 a          697 c          749 g         1658 t
ORIGIN      
        1 ttttaattaa gccaaaaata tcaaaagaag aatacataaa atgtattcat caattaaaac
       61 aacatattca aagaggcgat tgctacgaga taaatttttg tcaagagttt tttgcaaaag
      121 atgtggcaat taatacaata gaagtgtata aaaaattaac aaccttatct gctacacctt
      181 ttgcctgtta ttataaatta aatgaacagt atttactgtg tgcaagcccc gaaagatttg
      241 tttgcaaaaa tggaaataaa ataatttctc aaccaataaa aggaacaata aaaagaaatt
      301 taaaagatag cttacaagat gatgtattaa aaaaacaatt acaaaacagc agtaaagata
      361 aaactgaaaa tgtgatgatt gttgatttag taagaaatga tttaagtaaa atttgtacta
      421 aaggctcagt aattgtagaa gaactttttg gtgtttatag ttttcctcag gtacatcaaa
      481 tgatatctac agttagtggc aacgtacaaa aaaatataaa tttttctgaa gttttacaag
      541 ctacttttcc tatgggcagc atgacaggtg ccccaaaaaa aagagtttta gaactaaccg
      601 aacaatacga aaaaagcaat aggggaattt atagtggaac tgttggatat attacacctg
      661 aaaataattt tgattttaat gttgtaataa gaagtataat gtataatgca gtcaataaat
      721 atttaagcta tatggttggt ggtggtataa ccattaacag caatgcagaa gatgaatacc
      781 aagaatgttt attaaaggca actgccataa aagaagtgct tacctaaacc accttttaca
      841 taaataaaaa attacagtac atttgcaaat tgcaggcaga tttgtttatt gttaagccta
      901 catcaaaaca taactaataa acgaaccaac caatattacc aatacaaaat atttggcctc
      961 gttttttaga tacagtgggt gtagtacaaa aaaaattata cgtaattatg tttacaattc
     1021 aacaagagtt agaaaaaaga atattaatta ttgatggtgc aatgggcacc atgattcaaa
     1081 ggtataaatt acaagaagaa gattatagag gtacccgttt tataaactgg cataaagatg
     1141 taaaaggaaa caatgattta ctaagcatta ccaagccagc agtaattact gctatacata
     1201 aagaatattt acttgcagga gcagatataa tagaaacaaa tacatttagt agtacaagca
     1261 tagcacaagc agattatgat atgcaagaat ttgcttatga attaaatgta gcatctgcaa
     1321 cctgtgcaaa aaatgcaatt aacgagtata ttaaagaaac caatacaact acacctaaat
     1381 ttattgcagg tgcaattggg cctcttaata aaacactaag tctttcgcct gatgtaaata
     1441 acccaggctt tagagcagta acttttgatg aagtggtagc tgcttacaca gaacaaataa
     1501 aaggtttaat agatggtgga gtagatattc ttttaattga aaccatattt gataccttaa
     1561 acgctaaagc agcaattttt gctgcaaaaa aatttttcag agaaaataat attgaaaaac
     1621 ccataatgat tagtggaact attactgatg caagtggtag aacccttagt gggcaaacct
     1681 tagaagcatt ttatatttcg gtaaaacatg caaacccttt aagtattggt ttaaactgtg
     1741 cactaggtgc caatgaaatg agagggcata tagaagagct ttcaaatatt gcttcctgct
     1801 atgtatctgc atatccaaat gcaggcttgc caaatgctat gggtgagtat gatgaagctc
     1861 cagaagaaac agcacacttt attgaagatt gggcaaaaaa taaatgggta aatattgtag
     1921 gtggttgttg tggcacaaca ccagagcata ttaaacatat tgctgaaaat gtaaaactat
     1981 tacagccaag aagtttacca gttttagaaa caactattta ataaagtttc cgctaaatat
     2041 agaatggcta ataagcttat tattattcaa actcataaat agagttaatt tttgttcctg
     2101 ctttcatttc aaaaacaggt ttaataatac catcatttaa gctataaacc cacccatgaa
     2161 gatgtggtat atcgccacct ttccatgcat tttgaataat agaagatttg gctaaattta
     2221 atacttgttc tttaacattt agttcaacca acctgtcata gcgtttttct tcatcagcaa
     2281 tattattcaa ctcttcttcg tatattcttt gtatatcctt aatatttctt aaccacatgt
     2341 ttaaaatata tttcaaatca gttttacaca tagatgcttt tacaccacca caaccataat
     2401 gcccacaaac aattacatgt tttactttta aatgattaat tgcataatct aaaactgcca
     2461 ttaaattaac atcggtattt actactaaat tggcaatatt tctatgtaca aaaatttcgc
     2521 caggttgtgt accagtaatc tgatcggcag atacacgact atcgctacaa ccaatccaca
     2581 aaaactctgg tgtttgaagt ttggcaaggt tggtaaaaaa atctggatcc acttctacct
     2641 tttcctttgc ccatgattta ttttctaaaa gcagctttag gtaagattgc acttgagttg
     2701 ttttataaag ttttcaaata aaaataattt gtatcaaaaa aaattacctg taactatatt
     2761 taatttatac aaatgtaacc ttaataaaat ttaaaaatta ttatgtacca tttaatttat
     2821 aaattaatat ttttattgaa caaaagggta gccattaatt atgaagttga cactagatac
     2881 aaatattgta actgacgatt tttttaatga aacaatatta ttaggaatta ccacaaccat
     2941 taaaaactat aagctttgtt tacttttaaa tacttttatg ggttttcatt ttaagttgaa
     3001 taataatatt gaaatttgta tttctaaacg caaaagaaaa tattatttta gcgtatatga
     3061 atctgtttta aaaaacagca cattagtaca ttacttatac cataaccaca acgatggaga
     3121 atatttatta cctgaattta aacatataga ttttttgtgg ttaatgaaag gagatatttt
     3181 agatactaca caggtagaag aaataaaaac tgctattaaa acattaaatg gtgtacaaat
     3241 ggtggcagag ctaaccaatg agcaaataaa aaccaaaggc aatatgatat tttaattttt
     3301 tttatgtggc aagaaataga aaataaatta tataaaaaat ttagcttcaa aaatttttct
     3361 gaagcatttg catttataac tcaagttgct ttagaagcag aaaaaatgaa tcatcatcca
     3421 aactggtcta actcatggaa tattgttgaa atttggctat acacccatga tatgggcaat
     3481 aaggttacag aaaaagatat tttactctcc aaaaaaatag atgcaattat tagcaaataa
     3541 ttcttaagat atttatttaa gaaaattttt gccatactta catttgtaga tagtatgcaa
     3601 gagcctaata aaaaaaaggt atttgattat tcattattag ttagaattta ttcttacgta
     3661 aagccttata aacattggtt ttattttagc ttagttctgg caatactaat agcaatatta
     3721 gctccaatac gtccatattt aattcaactt actatagata aagctacagg taagaatata
     3781 ataattccta cttggttaca atttatcttt ccatcaaaaa atttaaacat agcggcacag
     3841 ttggttattt ggataaccat ttttcaaatt gtatttataa ttttcgaaac tattatcaga
     3901 tttgtttttt cttatttaac tgcaaagctt gggcaacata ttgtaaaaga tttaagggtt
     3961 agcgttttta aaaaaatatt acacctaaga ttaaggcagt ttgataaaac accaataggc
     4021 actttaacca ccagaaccat caatgacatt gaaagtatta atgatatttt tagcgatgga
     4081 ttaataccca ttatttcaga tttgcttaca attattttta cagttgcaac aatgttttgg
     4141 attaattggc aattagccct cattgcttta attccatttc catttataat tatagcaacc
     4201 tactatttta aagaaagtgt taatgcaagt tttatacgag taagaaatgc tgtagctaac
     4261 ttaaatgcct ttgttcaaga gcatcttaca ggaataaata ttgtacaagc ctttgcagca
     4321 gaagaaaaag agtttaaaaa atttgaagca ataaatcagg aacatagaag tgcaaacatt
     4381 aaagcaattt ttgcgtatag cgtttttttt cctgtagttg agttggtttt agcagttagt
     4441 attgggctta cagtttggtt tattgcaggc aaacaactta atcctggttt attaattgca
     4501 tttattttat accttaatca aatttttaaa cctttaagaa ttattgcaga taaatttaat
     4561 gttctacaaa tgggcatgat tgctgccgaa agagttttta aagttttaga taataaagat
     4621 gttgaaccta caaacaatac ttctgcaaac attttacaac taaaaggaga tataactttt
     4681 aataatgttt ggtttgcata taaaccacaa caatacgttt taaaaaatat ttcatttaat
     4741 gtacaacagg gcgaaacagt tgcattaatt gggcatacag gtagtggtaa aacatcaatt
     4801 ataagtatat tgaataaact atacgatata gaaaagggcg aaatattaat agatgatttt
     4861 aatattgata aaattaacat aagtacatta agaaaaaata ttgcagttgt tttacaagat
     4921 gtttttcttt ttgcaggaac tataaaagac aatattacac ttgaaaataa acttataaca
     4981 aatgaaaaag ttatagaagc tgctaagcta attggtgtac atgattttat aatgaagcta
     5041 cca
//
LOCUS       DAOQHP010000140         5031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9566_length_5031_cov_3.261053, whole genome shotgun sequence.
ACCESSION   DAOQHP010000140 DAOQHP010000000
VERSION     DAOQHP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_5031_cov_3.261053"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1450
                     /locus_tag="PLY81_05670"
     CDS             104..1450
                     /locus_tag="PLY81_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF29748.1"
                     /translation="MKQQKNNAWQKYQSPIQEFVNEASELINGIMVNDVDKYNYLQQL
                     AKELSATKDPYRRDVLSTLAKAIQTANPLSPGFEEANNYYKKLLQDNANIYNTHLYSE
                     TDKSALKVPEVKPFISFDAPSVNGYEVLNKYFDALFESNPKVFAFGEDVGKIGDVNQG
                     FSGLQEKYGSNRISDTGIRELTIMGQGIGLALRGLRPIAEIQYLDYLLFGLQPLSDDV
                     ATTHYRTRGIQSCPVIIRTRGHRLEGIWHSGSPMGMIINALRGMYVCVPRNMVQAVGM
                     YNTLLQSNDPGLIIECLNGYRLKEKLPANLLECTVPLGVPEIIKEGNDITIVSYGSTL
                     RIIQEAAERLNIENINCEIIDVQTLLPFDRHHIIIESLKKTNRIVFIDEDVPGGATAF
                     MFREVMEIQGGYKWLDVAPRTITAKAHRPSYGSDGDYFSKPNAEEIMNTIREMMAE"
     gene            1501..1923
                     /gene="aroQ"
                     /locus_tag="PLY81_05675"
     CDS             1501..1923
                     /gene="aroQ"
                     /locus_tag="PLY81_05675"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNF29749.1"
                     /translation="MKIAIINGPNLNLLGSRETKIYGTQTFENFIEQLEKQYPNIDFH
                     YYQSNVEGELINELQQVGFEYNGIILNPGGYTHTSVAIGDCIAAITTPVVEVHISNIF
                     AREEFRKISHISAKCAGTITGLGLKSYELALKWLIENK"
     gene            2060..2659
                     /locus_tag="PLY81_05680"
     CDS             2060..2659
                     /locus_tag="PLY81_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF29750.1"
                     /translation="MKNFTTVLISVLTIAVGVLFFLHFSSKKTPSLNNSNTNAVVANA
                     SGFKIAYFEMDSIENHYEYLKDVRNSLRSLEQRKSGELASLRNAGKSKIMEYQKKGNT
                     MTEQEIAMANEEIMRLENELKAQEQIKSQELQDESIRKIQDVKKTIEDFLKEYNKGKN
                     FSYILSSSSDIIYLKDTAYNITSDLIEGLNKNYKEKKKP"
     gene            2750..4174
                     /locus_tag="PLY81_05685"
     CDS             2750..4174
                     /locus_tag="PLY81_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF29751.1"
                     /translation="MSNNSFKPTLGLTDATMIVAGSMIGSGIFIVSADIVRNTGSAGW
                     LILVWLLTGFMTLIAALSYGELSAMFPKAGGQYVYLKEAYNPLIGFLYGWSFFAVIQT
                     GTIAAVGVAFAKFLAYLVPELGNSYFLFQIGFLKIYSGQLVAIVIIILLTYVNSRGVK
                     EGKWIQTLFTSAKLLALIGLIVFGFVLVKESYWDENWKTGFNSLQNKGIKDAHGLLQP
                     ADWIGISGSALMGAIAASMVGSIFSSDAWNNVTFIAGEMKNPQRNIGLSLFLGTLIVT
                     IIYVSANLMYLNVLPLNELAFPTDDRVAVAAANKIFPSFGTTFIAVLIMVSTFGCNNG
                     LILAGARVYYTMAQDGLFFKKAATLNKNNVPQWALWAQCIVASVLCLSGKYGDLLDMI
                     SFVVVIFYALTIWGIFILRKKQPNVIRPYKAFGYPFLPLLYIIMGLAFCVLLVIYKPM
                     YTWPGLIIVLIGIPIYYIAVGKKK"
     gene            4178..>5031
                     /locus_tag="PLY81_05690"
     CDS             4178..>5031
                     /locus_tag="PLY81_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNF29752.1"
                     /translation="MKFEELFRKIKQLKVGIVGDVMLDTYMWGNVERISPEAPVPIVS
                     LKNKENRIGGAGNVALNCIALQANTHIFSVIGNDEDGNKLIQLLNENNIDTGGIIKSS
                     NRITTNKLRIIARNQQMMRLDAEITNDINAIEECELLTKLELFIATQNPDVIIFEDYN
                     KGVLTEKLIAKAIQLCKENDIITSVDPKHKNFFNFKGVTIFKPNFKETIEALGIVDNI
                     ISLEQLNKIHHQLKEKLQHHISFITLSEKGVYYNSGNEANLMEAHIRKIADVSGAGDT
                     VIAVASLV"
BASE COUNT         1848 a          664 c          887 g         1632 t
ORIGIN      
        1 aaaagaatgg gattgaatta agaaaatgag agaatgaatt acaacaaaag gcatagcaac
       61 caatgaaaag ttagatcaaa ttcacgaaaa tgcaaaagaa gatgtgaagc aacagaagaa
      121 taatgcatgg caaaaatacc aatctcctat tcaagagttt gtaaatgaag cttcagaatt
      181 aattaatggt ataatggtaa atgatgtaga taaatacaat tatttacaac agttagctaa
      241 agagttaagt gcaacaaaag atccttacag aagagatgtg ttgagtactt tagccaaagc
      301 aattcaaact gccaatccac tttctcctgg ttttgaagaa gcaaataatt actacaaaaa
      361 gctattacaa gataatgcta atatttataa tacacattta tatagcgaaa ctgataaatc
      421 tgcattaaaa gtgccagaag taaaaccgtt tatttccttt gatgcacctt cagtaaatgg
      481 ttatgaagtt ttgaataaat attttgatgc tttgtttgaa tctaacccaa aagtttttgc
      541 atttggagaa gatgtgggca aaattggcga tgttaaccaa ggcttctccg gcttacaaga
      601 aaagtatgga agcaacagaa tttctgatac tggtattaga gaactaacta taatggggca
      661 aggtattggt ttagctttaa gaggcttaag accaattgca gaaatacaat atttagatta
      721 tttgttattt gggttacaac ctttaagcga tgatgtggca actactcact atagaacaag
      781 aggtattcaa agttgcccag ttattattag aacaagagga catagattag aaggcatttg
      841 gcatagtggt agcccaatgg gtatgataat aaatgcttta agaggtatgt atgtttgtgt
      901 accacgaaat atggtgcaag ctgttggcat gtacaacaca cttttacaaa gtaacgatcc
      961 aggcttaatt atagaatgct taaatggcta tcgtttaaaa gaaaaactgc ctgctaattt
     1021 attagagtgt acagtgcctt taggtgtacc tgaaataatt aaagaaggta atgatattac
     1081 aattgtttct tatggctcta ctttaagaat tatacaagaa gctgctgaaa gattaaacat
     1141 tgaaaatatt aattgcgaaa taatagatgt acaaacatta ttaccttttg acaggcatca
     1201 tataattata gaatctttaa agaaaacaaa ccgtattgtg tttattgatg aggacgtacc
     1261 tggtggggca actgctttta tgtttagaga agtaatggaa atacaaggtg gttataaatg
     1321 gttagatgta gcaccaagaa ctattactgc taaagctcac aggccaagtt atggcagcga
     1381 tggagactat tttagcaagc ctaatgcaga agaaataatg aatacaatta gggagatgat
     1441 ggcagaataa attatgtatc aactagatta actcatctat ttcttcaaga aagttttact
     1501 atgaaaattg caattatcaa tgggcctaac ttaaacttat taggctcaag agaaactaaa
     1561 atatacggca cccaaacatt tgagaatttt attgaacaat tagaaaaaca atatccaaat
     1621 attgattttc attactatca aagtaatgta gaaggcgaat taataaatga attgcagcag
     1681 gttggatttg agtacaatgg aataatttta aacccaggtg gatacacaca tacttctgtt
     1741 gccattggcg attgtattgc agcaattact acgcctgttg tagaagtaca tattagcaat
     1801 atttttgcca gagaggaatt tagaaaaata tctcatataa gtgcaaaatg tgcaggaact
     1861 attactggcc ttggtttaaa aagttatgaa ctagcattaa aatggctgat tgaaaataag
     1921 taaccttaat ttttagcaca ctattatatt tgtagcctct cttttaacaa ataaataatt
     1981 aatgtaaaac tacatttgcc cataaatact tttcaaaaca agcaataacc aaacaattat
     2041 atcatttaaa aaataattca tgaagaattt tacaactgta ttaattagtg ttttaaccat
     2101 tgcagtaggg gttttatttt ttttacattt ctcctctaaa aaaactcctt ctttaaataa
     2161 tagcaatacc aatgcagttg tagcaaatgc cagtggtttt aaaatagctt attttgaaat
     2221 ggactctata gaaaaccatt atgaatattt aaaagatgta agaaacagtt taagaagttt
     2281 ggaacaaaga aaaagtggcg aacttgcttc tttacgcaat gcaggcaaat ctaaaattat
     2341 ggagtaccaa aaaaaaggta acaccatgac agaacaagaa attgcaatgg caaatgaaga
     2401 aataatgagg ttagaaaatg aattaaaagc tcaagagcaa ataaaatcgc aagagttaca
     2461 agatgaaagt atccgaaaga ttcaagatgt aaaaaaaaca attgaagact ttttaaaaga
     2521 atacaataaa ggcaaaaact tttcttacat tttatccagc agttcagata ttatttatct
     2581 aaaagacact gcatataaca ttactagtga tttaattgaa ggcttaaata agaactataa
     2641 agaaaagaaa aaaccataaa taatatatta gctttgtatt attataaaat ctttatcttc
     2701 aaatcccatt taattaatca aatgggattt tttaatacca aaactattta tgagtaataa
     2761 ttcatttaaa ccaactttag gcttaacaga tgcaactatg attgttgctg gtagtatgat
     2821 tggttcagga atttttatag taagtgcaga tattgtaaga aatacaggca gtgctggctg
     2881 gctaatttta gtttggttgc ttactggctt tatgacatta attgcagccc ttagctatgg
     2941 cgaattaagt gccatgtttc ctaaagctgg tggacagtat gtttatttaa aagaagctta
     3001 taacccatta attggttttt tgtatggctg gagttttttt gcagtaattc aaactggtac
     3061 tattgcagca gttggggttg cttttgctaa atttttagct tatttagttc cagaattggg
     3121 caatagttac tttctttttc aaataggctt tcttaaaata tactctgggc aattagttgc
     3181 cattgtaatt attattttac taacctacgt taattcaaga ggtgtaaaag aaggaaaatg
     3241 gatacaaaca ctttttacaa gtgcaaagct tttggcatta attggtttaa ttgtttttgg
     3301 atttgtttta gtaaaagaaa gctattggga tgagaattgg aaaaccggat ttaactctct
     3361 acaaaataaa ggtattaaag atgctcatgg cctattacaa cctgcagatt ggattggtat
     3421 tagtggcagt gctttaatgg gtgccattgc agcatctatg gttggatcta tttttagtag
     3481 cgatgcctgg aataatgtaa cttttattgc aggagaaatg aaaaatccac aaagaaatat
     3541 tggcttaagt ttatttttag gaaccttaat tgttaccata atttatgtga gtgccaattt
     3601 aatgtactta aatgtattac cattaaatga attggctttt cctacagatg atagagttgc
     3661 agtagcagca gcaaataaaa tattcccttc ttttggtaca acatttattg ctgtacttat
     3721 tatggtatca acctttgggt gcaacaatgg tttaatattg gctggtgcaa gagtttatta
     3781 tactatggca caagatggat tgttttttaa aaaagctgct actcttaata aaaacaatgt
     3841 tcctcaatgg gcattatggg ctcaatgtat tgtagcttct gttttatgct taagtggtaa
     3901 gtatggagat ttgttagata tgatttcttt tgttgtggta attttttatg ccctcaccat
     3961 ttggggaatt tttattttaa gaaaaaaaca acccaacgtt ataagacctt ataaagcctt
     4021 tggctatcct tttctacctt tactatatat tataatggga ttagcatttt gtgtactgtt
     4081 ggttatttat aaacccatgt acacttggcc tggattaata atagttttaa ttggaattcc
     4141 tatttattat attgctgttg gtaaaaaaaa ataattcatg aagtttgaag aactctttag
     4201 gaaaataaaa caattaaaag ttggtattgt tggcgatgta atgttagata cttatatgtg
     4261 gggaaatgta gaaagaattt cgccagaagc tcctgtgcca attgtttctt taaaaaacaa
     4321 agaaaataga attggtggtg caggtaacgt tgctttaaac tgtattgctt tacaagctaa
     4381 cacacatatt ttttcagtaa ttggaaatga tgaagatggt aataaactaa ttcaattatt
     4441 aaatgaaaat aatattgata ctggtgggat aattaaaagc agtaacagaa ttacaactaa
     4501 taaacttaga attattgcaa gaaatcagca aatgatgcgt ttagatgctg aaattaccaa
     4561 tgatattaat gccattgaag aatgtgagtt attaactaaa ttagagttat ttattgcaac
     4621 acaaaatcca gatgtaatca ttttcgagga ctacaataaa ggagttttaa ctgaaaaatt
     4681 gatagcaaaa gcaatacaat tatgtaaaga aaatgatatt atcacttctg ttgaccctaa
     4741 acataaaaac tttttcaact ttaaaggtgt tactattttt aagcccaact ttaaagaaac
     4801 catagaagca cttggcattg ttgataatat aattagcttg gaacaattga acaaaataca
     4861 ccatcaatta aaagaaaagt tacaacacca tatatcattt attactttat cggaaaaagg
     4921 tgtttattac aactcaggaa atgaagccaa tttaatggaa gcccatataa gaaaaattgc
     4981 agatgtaagt ggtgctggcg atacagttat tgctgtagca tctttggtat a
//
LOCUS       DAOQHP010000141         5014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9616_length_5014_cov_3.905424, whole genome shotgun sequence.
ACCESSION   DAOQHP010000141 DAOQHP010000000
VERSION     DAOQHP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_5014_cov_3.905424"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4210
                     /locus_tag="PLY81_05695"
     CDS             <1..4210
                     /locus_tag="PLY81_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF29753.1"
                     /translation="GNTNLTTTVTVKPVYTFNGKSCDGIAETYTITITPSTPTAAAGN
                     DTILCSANTYTMQATLAGGSTGVWSEFGGNSTTIQNTTSPTTQITGLLPNNTYKYVWN
                     VTGVASCPGTSDTITIINYASVVNQIDNTTKVICATQTININGNSPTGGSGSYTYQWQ
                     QSTDGGLTWTNIPFKTSKDLSYIPTTSVLIRRIVYSLPCIEQSSTTIVTVQPAISNNT
                     ISANQSICINTVPTTLIGTVATGGDGNISYQWQLSTNGGITWSDISGATSKDFSPSAL
                     TVTTKYRRVVSTSLCFGTQGNNSLAITITVNPDSHANYTFTNNIGCAPFLINSINIKP
                     TLLPLQNGTYNWYANNTYLVGGPSFPGFTLTNPGDSIQIKLVTTSPFGCKADSVTHKF
                     YTVAKPQTSFTASDTIGCGPLNVLFTNTTPNINWFNYQWSFGNGLTSNLPQPNTVTFA
                     INPTFFDTIYTVQLSATTVCQTVKDTQYIRVKSKPKAVFTPNKSVGCSPMTVFFNNTS
                     KGNNVQYKWNFGDGGADTLVTTKSSMSYTYYRGFQDTVDVRLIAFNDCGSDTQTYKIV
                     ISPNLIQLDFAVNGNETQGCKPKSVKFINASNGATNFFWSFGDGNTLSTTKNIDTVTH
                     TYTTAGTFIATLKASNNCSDTTSVETIQIFNTPIINFTAVPSSICIGDTIHFTNLTDT
                     ASSYLWSFNDNSTSTLVNPKHIYPVAGNYLVQLKALRQYNSGLTCIDSISKPISVVSK
                     LKGSFTANDTISPCVPFTLSMVNNTPALQTIWYSNNIPIDTGNVATYTFNQVGSYLIK
                     MNATNAAGCIKEDTQTVVVRGPSGTFQYDHGIICSNNAVYFEVLPSNTDSIKFNFGDG
                     NFLTTTANSVYHQYNQTGSFIPSVILYSNFSPACTVTLQGTDTIKIDYVKAGFKYSSQ
                     TYCDSTKIFFNDTSRHALPIVSWQWNFGDGNFSTLQHPTHTYKNTNNWAVQLITKTVG
                     GCTDTVNYNMFVKVNNKPSNTILAPLVACALKPINYQAQTTSVDSIIFNNWQFNNGLT
                     TLGNNVNNTYILPGTYYATLISGTAYGCTDTALHTITINPTPTVVTTSNQIICLGSSI
                     QLNVSGANTYSWAPINNTLSCLTCINPIATPITSTMYIVEGTNTFGCSNKDSVMVTVA
                     QPITIQTSANDTICIGQSAQLSATGATTYQWMPSSSLQNNNTANPIATPAITTTYQVI
                     GFDNYNCFQDTGFITVAVGKYPTITLEADKVLPTGTQLALKSTVTNGPITNWLWTPSA
                     DLNCNTCALPIATIKNDVCYTVTAANIYHCAATDTICIKTFCEGTQVFIPNAFTPDGD
                     GINDVLMVRGSGIKTVKSFRIFNRWGQVVFEKTNVTPNDPAQGWNGTINGKPASPEVY
                     VYTCEVVCENNVAYTYKGNVAIIK"
     gene            4325..>5014
                     /locus_tag="PLY81_05700"
     CDS             4325..>5014
                     /locus_tag="PLY81_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF29754.1"
                     /translation="MKFLLFLCTSLVCCINNYAQDFTFSQFYEKPLLRNPSLAGVFDG
                     DLRVTGIYRNQWQSVTVPFNTSGASVEVKFPVGKGNDFLTVAMQMTNDVAGDIKLKRT
                     QILPVINFHKSLNDEYNSYLSLAFMAGNVSSRFDPSKLQMNDQFVNGSFNPGNPTMQS
                     FSKTSINYWDLGAGLTYSFDFGESSSLYFGASLFHLNKPKVSFYANNANSTTLHNKLS
                     LNAGLTTPTSDY"
BASE COUNT         1762 a          857 c          800 g         1595 t
ORIGIN      
        1 aggaaatact aatttaacaa caacggttac agttaaacca gtttatactt ttaatggtaa
       61 aagctgcgat ggcattgcag aaacttacac tattaccatt acaccatcta ctccaactgc
      121 tgcagcaggt aatgatacta tactttgttc tgccaatact tataccatgc aagcaacact
      181 tgcaggtggt agtactggtg tttggtcaga gtttggagga aattcaacaa ccattcaaaa
      241 tacaacttct ccaacaacac aaataacagg attacttcct aataatacat acaaatatgt
      301 ttggaatgtt acaggggttg caagctgccc aggtacttca gatactataa ctattataaa
      361 ttatgcatct gttgttaatc aaatagataa taccaccaaa gttatttgtg ctacccaaac
      421 tataaatatt aatggaaact cacctacagg tggcagtggt agttacactt atcaatggca
      481 acaaagtaca gatggaggat tgacctggac aaacatacct tttaaaacta gtaaagactt
      541 atcttatata ccaactactt ctgtattaat aagaagaatt gtttattcgt tgccatgtat
      601 tgaacaaagt agcacaacaa ttgttacagt gcaacctgct attagtaaca atactatttc
      661 tgcaaaccaa agtatttgta ttaatactgt tccaacaacg ttgataggca ctgttgcaac
      721 aggtggcgat ggaaacattt cgtatcaatg gcaactaagt accaatggag gtataacatg
      781 gagtgatatt tctggagcaa cctcaaaaga tttttctcca tcggcactaa cagttacaac
      841 aaaatataga agagtagtaa gcactagttt atgttttgga acacagggta ataatagttt
      901 ggcaattaca attacagtta atccagattc tcatgcaaat tatactttta caaacaacat
      961 aggctgtgca cctttcttaa ttaattcaat aaatattaaa ccaactttat tgccattaca
     1021 aaatggcacc tataactggt atgcaaataa tacgtattta gttggtgggc ctagttttcc
     1081 tggttttaca ttaacaaatc ctggcgatag cattcaaata aaattagtaa ctacaagtcc
     1141 gtttggttgt aaggcagata gtgtaacaca taaattttat acagttgcaa aacctcaaac
     1201 atcatttaca gcgagtgata ctataggttg tgggcctcta aatgttttat ttactaatac
     1261 tacacccaat ataaattggt ttaattatca atggtcgttt ggcaatggat taacttctaa
     1321 tttaccacaa ccaaatacag taacttttgc aatcaaccca actttttttg atactattta
     1381 tactgttcaa ctttctgcaa caactgtatg ccaaacagta aaagatacac aatatattag
     1441 ggtaaaatca aaaccaaagg cagtttttac acctaataaa tctgttggtt gctcaccaat
     1501 gactgtgttt tttaacaaca cttccaaagg taataatgta caatataaat ggaactttgg
     1561 agatggagga gccgacactt tagttactac taaatcctca atgtcttata cttattacag
     1621 aggatttcaa gatactgttg atgttaggtt aatagctttt aatgattgtg gtagtgatac
     1681 gcagacatac aaaattgtaa tttctcccaa cctaattcaa ttagattttg cagttaatgg
     1741 taatgaaact caaggttgta aacccaaatc ggttaaattc attaatgcat ccaatggggc
     1801 aactaatttc ttctggagtt ttggagatgg taatacatta agtacaacaa aaaatataga
     1861 tactgttacg catacttata ctactgcagg tacatttatt gcaacactaa aagcaagtaa
     1921 taattgtagc gatacaacta gcgttgaaac tattcaaatt tttaataccc caattataaa
     1981 ctttacggca gtaccatctt ccatttgtat tggagataca attcacttta caaaccttac
     2041 agatactgca tctagttact tatggagctt taatgataac agcacatcaa cacttgttaa
     2101 tccaaaacat atataccctg tagctggcaa ttatttagta caattaaaag cattaaggca
     2161 atataatagt ggcttaactt gtatagatag tattagtaaa cctatttctg tagtttcaaa
     2221 attaaaaggt tcttttacag caaatgatac aattagccct tgtgttcctt ttacattatc
     2281 aatggtaaat aatacaccag cacttcaaac catttggtat agtaataata tacctattga
     2341 tacaggcaat gttgctacat acacttttaa tcaggtaggt agttatttaa taaaaatgaa
     2401 tgcaactaat gctgctggtt gtataaaaga agatacgcaa actgttgtag ttagaggccc
     2461 atctggaact tttcaatatg atcatggcat tatctgctct aacaatgctg tttattttga
     2521 agtattgcca agcaataccg atagtattaa gtttaatttt ggcgatggta attttttaac
     2581 caccactgcc aatagtgtgt atcatcaata taatcaaacg ggtagtttta ttccatcagt
     2641 aatattatat tctaattttt cacctgcttg tactgttaca ttacaaggca cagatactat
     2701 taaaattgat tatgtaaaag cagggtttaa atattcatca caaacctatt gcgactcaac
     2761 aaaaatattt tttaatgata cttctaggca tgccttacct attgttagtt ggcaatggaa
     2821 ttttggcgat ggcaatttta gtactctaca acatccaacc catacttata aaaataccaa
     2881 caattgggcg gtgcagctaa ttactaaaac tgttggaggt tgtacagata cagtgaatta
     2941 taatatgttt gtaaaagtaa acaacaaacc atctaatact atacttgcac ctttggtagc
     3001 atgtgcatta aagccaataa attatcaggc acaaacaaca agtgtagata gtataatatt
     3061 taataattgg caatttaata atgggctaac aacacttggc aacaatgtaa ataataccta
     3121 cattttacca ggtacttatt atgctacttt aatttctggt acagcttatg gttgtacaga
     3181 tacagctcta cataccatta caataaatcc taccccaact gttgttacaa catctaatca
     3241 aataatttgt ttaggtagtt ctattcaatt aaatgtatct ggtgcaaata cttatagctg
     3301 ggcaccaatt aataataccc ttagctgttt aacatgtatc aatcctattg ctacacctat
     3361 tacatctacc atgtatattg tagaaggtac taatactttt ggatgtagta ataaagatag
     3421 cgtaatggta actgttgcac agcctataac aatacaaaca tctgccaatg atacaatatg
     3481 tattggtcag tctgctcaac ttagtgcaac tggtgcaaca acttatcaat ggatgccttc
     3541 ctcaagcttg caaaataata atactgcaaa ccctattgct acacctgcca ttactacaac
     3601 ttaccaagta attggattcg ataattacaa ttgttttcaa gatacaggct ttataactgt
     3661 agctgtagga aaatatccaa ccataacatt ggaggctgat aaagtattgc caacaggtac
     3721 tcaattagca ttaaagagca ctgtaactaa tggccctatt actaattggc tatggactcc
     3781 atcggcagac ttaaattgta acacttgtgc tttgccaatt gcaactatta aaaacgatgt
     3841 ttgttatact gtaacagctg ccaatatata tcactgtgca gctacagata caatttgtat
     3901 taaaacattt tgcgaaggaa cccaagtatt tatacccaat gcctttacac cagatggaga
     3961 tggtataaat gatgtattaa tggtaagagg cagcggaatt aaaactgtga aaagttttag
     4021 aatatttaat agatgggggc aggtggtttt tgagaaaact aacgttacac caaacgatcc
     4081 tgcacaaggt tggaatggaa caattaatgg caaaccagct agtccagagg tttatgttta
     4141 cacttgtgaa gttgtttgcg aaaataatgt tgcttatact tacaagggta atgtagcaat
     4201 tataaaataa tggctttaaa taaagaatta taggaaaatg aaaaatatta ttgtaattga
     4261 atggaagaat taattatata taaatttata agattgccaa tggcagaagc acaaaaataa
     4321 acacatgaaa tttttacttt ttttatgtac ctcgttagta tgctgcataa ataactatgc
     4381 acaagatttt actttttcgc aattttatga aaaaccatta ttgcgtaacc cttcattggc
     4441 aggtgttttt gatggagacc taagagttac aggtatatat agaaaccagt ggcaaagtgt
     4501 aacagtgcct tttaacactt caggtgcaag tgtagaagta aagtttcctg taggtaaagg
     4561 caacgatttt ttaaccgttg caatgcaaat gaccaatgat gttgcaggag atattaaatt
     4621 aaaaagaaca caaattttac cggtaattaa ttttcataaa tcactaaatg atgagtacaa
     4681 cagttattta tcattagcat ttatggcagg caatgtaagt agcaggttcg atccaagtaa
     4741 attacaaatg aacgatcagt ttgtaaatgg ctcttttaat ccgggcaacc caactatgca
     4801 aagctttagc aaaacaagta ttaattactg ggatttaggt gcaggcttaa cctatagttt
     4861 tgattttggc gaaagcagta gcctttattt tggagcttct ctctttcatt taaacaaacc
     4921 aaaagtttcc ttctatgcca ataatgctaa ttctacaaca cttcataata aattatcctt
     4981 aaatgctggt ttaacaacac ccacaagcga ttac
//
LOCUS       DAOQHP010000142         4992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9674_length_4992_cov_3.754912, whole genome shotgun sequence.
ACCESSION   DAOQHP010000142 DAOQHP010000000
VERSION     DAOQHP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9674_length_4992_cov_3.754912"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLY81_05705"
     CDS             complement(<1..315)
                     /locus_tag="PLY81_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29755.1"
                     /translation="MKKVNKQILTKQLLFLIFFLSIVLASTAQLPANLSRVKSSDITD
                     SQLLEFIEKAKGSGLSEEQVIQELQRRRMSFAEIEAIKGRIAALVSPSKTNTSSAAMH
                     ESR"
     gene            complement(569..1861)
                     /locus_tag="PLY81_05710"
     CDS             complement(569..1861)
                     /locus_tag="PLY81_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNF29756.1"
                     /translation="MKLEESKIAIIGLGYVGLPLAIEFGKKFSVLGFDINQNRISELV
                     EGKDRTNEADLQGLKQATLLYNSTNGTKGLFFSSDINLLKECTVFIVTVPTPINNFKA
                     PDLTPLIAASATISKVLKPNDIVIYESTVYPGCTEEDCVPVLEKYSGLKFNKDFYCGY
                     SPERINPGDKVNTLTKIKKVTSGSTPAIANFVDDLYASIITAGTHKAPSLKVAEASKA
                     IENAQRDVNISFVNELALIFDKMGIDTTDVIEAAATKWNFLKYKPGLVGGHCIGVDPY
                     YLAHKAESLGYHPQVILSGRRVNDNMGLFVASKVVKLMIAKGQKVNGAKALILGITFK
                     ENCPDIRNSKVIDIYNELKQYGMDVDVYDTHASKEEVKEEFGINLIDSLSKKYDALIL
                     AVSHNEFMHLNINQLIATDAVIFDTKAFLDRSIVDARL"
     gene            complement(1914..2393)
                     /gene="ispF"
                     /locus_tag="PLY81_05715"
     CDS             complement(1914..2393)
                     /gene="ispF"
                     /locus_tag="PLY81_05715"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNF29757.1"
                     /translation="MSFRIGFGIDFHQFAEGRDLWLGGIKIPHSKGAIGHSDADVLLH
                     AICDALLGALALGDIGLHFPNTEQAYKDIDSKILLKKSYELIKEKGYTIINIDSTVCL
                     ENPKIKPFVSSMQQTIAEILDITVNDISIKATTTETMGFAGREEGLVAYATVLLQKQ"
     gene            complement(2482..3654)
                     /gene="porV"
                     /locus_tag="PLY81_05720"
     CDS             complement(2482..3654)
                     /gene="porV"
                     /locus_tag="PLY81_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNF29758.1"
                     /translation="MRHSIWRVTALFLLTCSFSNVLKAQSTDINVVTTAVPFLRISPD
                     ARAGGMGDVGIATTPDANAPFWNLSKVPFTTKNTEISLTYTPWLKDLGLTDVYLASFA
                     GYHKLDEEQAVAASIRFFSLGNIQFTDYAGNPLQENRPREFSIDAGYTRKLNTKLSMG
                     VTLRYINSQLANGSIGGVNYKAGNAISGDISLYNYGLSEKGEGLSWGVVLSNLGTKIS
                     YTNDATNKDYIPANLGLGVAYTTVFDETSKLTFGLDVNKLMVPTPPTPVDPNEPNADA
                     RNAALLEEYRSMSVMKSWFKSFGDSGGFGNELKEFQVSLGAEYVYNDQFALRLGYFYE
                     DKSKGNRKFFTVGAGLKYNVIGINLSYLVPSGSGVTRNPLSNTLRFGLTFDIADKK"
     gene            4034..>4992
                     /gene="gatB"
                     /locus_tag="PLY81_05725"
     CDS             4034..>4992
                     /gene="gatB"
                     /locus_tag="PLY81_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNF29759.1"
                     /translation="MGEISKYEAVIGLEIHAQLQTNSKLFSGDSNHFEMEPNSNISPI
                     VLAHPGTLPKTNKKAVEYAIKMGLACNCSIEKNNYFARKNYFYADLPKGYQISQHTTP
                     ICKGGYVTIQMNENEEKKIQLNRIHLEEDAGKSIHDQHPNYSLIDFNRAGTPLIEIVT
                     EPCMNNSNEAYKYVNEIRKLVQWIDICNGNMEEGNLRCDANVSIRKKGEITLGTKVEV
                     KNLNSVRNIKKAIDFEIERMIALAEKGEKITQQTRSFDADTDTTFALREKEDTNDYRY
                     FADPDLAPFYISDNDINKLKNNLPVLPNLLKQKFIREFNLSLY"
BASE COUNT         1714 a          853 c          699 g         1726 t
ORIGIN      
        1 tctgctttca tgcattgcag cactggatgt attagtttta cttggagaga caagtgctgc
       61 aattcttcct ttaatggctt ctatttcagc aaaactcatt cttcttcttt gcagttcttg
      121 tattacttgt tcttctgata aaccagagcc ttttgccttt tcaataaatt caagcagttg
      181 actatcggta atatcagaag attttactct gcttaaattg gcaggcaact gtgcagtaga
      241 tgccaatact atacttaaaa aaaatattaa aaagagtaac tgctttgtaa gaatttgctt
      301 atttactttt ttcatgagtt tgatatttta gatattaacc aataccttgg ttagcatttg
      361 aattatagta agaaataaaa aattatacag ctccgccacc ttactcacaa attaggtgag
      421 tagcctttat taagaaataa gattttagtc aaaatttctt ttattaaaaa aataatttaa
      481 aggtggcaaa tataagtcta agcctatttt aaattattat aattttattt tataaaaagt
      541 attgtataaa attccaactc ctctaaaatt ataaccttgc atctacaata cttctatcta
      601 aaaatgcttt ggtatcaaaa attacagcat ctgttgctat tagttgatta atgtttaaat
      661 gcataaactc attatgtgat acagctaata tcaatgcatc gtatttttta gataagctat
      721 ctattaaatt aattccaaac tcttccttta cttcttcttt gctggcatgg gtatcatata
      781 catctacatc cataccatat tgtttcaatt cattataaat atctatcact ttagagtttc
      841 taatatcggg gcaattttct ttaaatgtta taccaagtat taatgctttt gcaccattta
      901 ctttttggcc tttagcaatc attaatttta caaccttact ggcaacaaat aatcccatat
      961 tatcatttac tcttctacca cttaaaatta cttgtggatg atagcctaag ctttcggctt
     1021 tgtgtgctaa atagtagggg tcaacaccaa tacagtgccc tccaactaaa cctggtttgt
     1081 attttaagaa attccatttt gtggcagcag cctcaataac atcggttgta tcaataccca
     1141 ttttatcaaa aattaaagcc agctcattta caaaagaaat atttacatct ctttgtgcgt
     1201 tttcaatggc tttcgatgct tctgcaactt ttaaacttgg ggctttgtgt gtgcctgcgg
     1261 ttataatgct tgcatataaa tcatctacaa aattggcaat agcaggtgtg gagccagagg
     1321 ttactttttt aattttggtt aaagtattta ctttatctcc tgggtttatt ctttctggcg
     1381 aataaccaca ataaaaatct ttattaaact ttaatcctga atatttttct aaaactggta
     1441 cacaatcttc ttctgtacat cctggataaa ctgtagattc gtaaattaca atatcattag
     1501 gctttaaaac cttactgatt gtggcagatg ctgcaattaa aggcgttaaa tctggagctt
     1561 tgaaattatt aattggggtt ggtacagtta caataaaaac agtacattct tttaataagt
     1621 ttatatcaga agaaaaaaat aaacctttag taccatttgt tgaattatac aataatgtgg
     1681 cttgtttcaa tccttgtaaa tctgcttcat tggttctgtc ttttccttct acaagctcac
     1741 taattctatt ttgatttata tcaaacccta atacagaaaa ttttttacca aattctattg
     1801 ccaatggtaa tcctacataa cctaagccaa taatggcaat tttactttct tctaacttca
     1861 tatatttatt attctaaaac ttattattaa attttttgtg gtaaaattat ttattattgt
     1921 ttttgcaaca aaactgttgc ataagccacc aatccttctt ctcttcctgc aaagcccatt
     1981 gtttcggttg tggttgcttt aattgaaata tcgttaactg taatatccaa aatttctgca
     2041 atggtttgtt gcatactact tacaaaaggt ttaatttttg gattttctaa gcaaacagta
     2101 ctgtcaatat ttataatggt atatcctttt tcttttatta attcgtacga tttttttaat
     2161 aaaattttac tatctatatc tttataagct tgctctgtat ttggaaaatg caaaccaata
     2221 tcgcctaatg ccaaagcccc taataaggca tcgcaaatgg catgtaataa aacatctgca
     2281 tcgctatggc ctattgcacc ttttgagtgg ggtattttga tacctccaag ccataaatct
     2341 ctaccctcgg caaattgatg aaaatctatt ccaaatccta ttctaaaact catttaaaaa
     2401 aatgtttact tataaaatac caaaagaagg catttcaaaa acaccttcta taaatttatc
     2461 tcaagaaata gttagtataa attatttctt atctgctata tcaaacgtta gtccaaagcg
     2521 aagtgtattt gaaagggggt tacgggttac accacttcct gatggaacta aataagataa
     2581 attaatacca attacattat attttaagcc tgcacctact gtaaaaaact ttcggttgcc
     2641 tttgctttta tcttcataaa aataacctag ccttaatgca aactgatcgt tgtaaacata
     2701 ttcggcccct aatgaaactt gaaattcttt taattcatta ccaaaaccgc cagagtcgcc
     2761 aaagctttta aaccaacttt tcatcacact catacttctg tattcttcta gtaaagcagc
     2821 atttcttgca tctgcattag gctcattggg atctacaggg gttggtggcg ttggcaccat
     2881 tagtttgtta acatctaatc caaaagttag tttacttgtt tcgtcaaaaa cagttgtgta
     2941 agcaacacct aaacctaaat tggcaggtat ataatcttta tttgtagcat cgttagtata
     3001 gctaattttt gtacctaagt tagataaaac aacaccccaa cttaaacctt ctcctttttc
     3061 actcaatcca tagttatata aagaaatatc accagaaatt gcattacctg ctttataatt
     3121 tactccacct atagacccat ttgctaattg agagttgata tagcgtaatg taacacccat
     3181 tgaaagcttt gtatttagtt tacgtgtata accagcatca atagaaaact ctctggggcg
     3241 gttttcttgt aatgggtttc ctgcataatc ggtaaactga atattgccta agctaaaaaa
     3301 acgaatagaa gctgcaacag cttgctcttc atctaactta tgatagcctg caaaactggc
     3361 taaatacaca tccgttaagc caaggtcttt aagccaaggc gtataagtta aactgatttc
     3421 ggtattttta gttgtaaaag gcactttcga taaattccaa aaaggtgcat ttgcatccgg
     3481 tgtagtagct attcctacat cacccatacc tcctgcacgt gcatctggcg aaattcttaa
     3541 aaatggtacc gcagttgtaa caacattaat atctgtactt tgggctttta atacatttga
     3601 aaatgaacaa gttaataaaa ataatgctgt aaccctccaa attgaatgtc tcatctaaat
     3661 caaaaaaatt taatatttgt aaaaataaaa gaatatttca accagccaaa tcttgctatc
     3721 tgaaatattg attaagtaaa aattagccca ttaaataaat ttggttacaa aaaactttag
     3781 taattaataa aaatacaaca cttaaaaagc attatttgtt tgcaataata caaaaatcag
     3841 acacttaaac agtatttttc gctgtttatt gtacaatttt ttattaattc tttacttaaa
     3901 taataatttt ctggttcaat taactactta tagccaatat aaataatata aacacttgtt
     3961 aaacattgta atagaagcta ataaatttct atttatataa ctgtgtacaa tacctttaca
     4021 gccatcttta tttatgggtg aaatatcaaa atacgaggct gttattggat tagaaattca
     4081 tgctcaattg caaaccaata gtaaattgtt tagtggcgat agtaaccatt ttgaaatgga
     4141 acctaattca aatataagcc caatagtttt agcacatcct ggcactttac caaaaactaa
     4201 caaaaaagca gtagagtatg ccattaaaat gggattagca tgtaattgta gcattgaaaa
     4261 aaacaactat tttgctcgta aaaattattt ttatgccgat ttgcctaagg gctaccaaat
     4321 ttctcaacat accacaccaa tatgtaaggg tggttatgtt accattcaga tgaatgaaaa
     4381 tgaagaaaag aaaattcaac ttaatagaat tcatttagaa gaagatgctg gcaaaagcat
     4441 acacgaccaa caccccaact acagtttaat tgattttaac cgtgcaggca ccccattaat
     4501 agaaattgtt acagagcctt gcatgaacaa tagtaatgaa gcatataaat atgtaaatga
     4561 aataaggaag ttggtacaat ggattgatat ttgtaatggg aatatggaag aaggcaactt
     4621 gcgttgcgat gccaatgtct ccatcagaaa aaaaggagaa ataacattag gtactaaagt
     4681 ggaagtaaaa aatttaaact ctgtaagaaa tataaaaaaa gcaattgact ttgaaattga
     4741 aagaatgatt gcactagcag aaaaaggaga aaaaattaca cagcaaacaa gaagttttga
     4801 cgctgataca gataccacat ttgctttgag agaaaaggaa gacactaacg attatcgtta
     4861 ttttgccgac ccagacttag ctccttttta catttctgat aacgatatta ataaacttaa
     4921 aaataactta cctgttttac caaacttgct taaacaaaag tttatacgtg aatttaattt
     4981 aagtttatac ga
//
LOCUS       DAOQHP010000143         4989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9682_length_4989_cov_3.653831, whole genome shotgun sequence.
ACCESSION   DAOQHP010000143 DAOQHP010000000
VERSION     DAOQHP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9682_length_4989_cov_3.653831"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLY81_05730"
     CDS             complement(<1..809)
                     /locus_tag="PLY81_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNF29760.1"
                     /translation="MDFPVLLIVIIALAFIFDYINGFHDAANSIATIVSTKVLTPFQA
                     VLWAAVFNFAAFFVSKYLIGEFKIGNTIAKSVHENYITLEVIFAGLMAAITWNLITWW
                     FGIPSSSSHTLLGGFIGASLAYAGAFSKNGIDVINYAKVLPTVLFIFIAPLIGMFIAF
                     LITILIVHIFKRSNHYKAESWFKKLQLASSAAFSLGHGGNDAQKVMGIIGAAIIYHES
                     TLGHSLSFNEFVIKYSWVPFCSFFAIGLGTLSGGWKIVKTMGTRITKVTPL"
     gene            complement(854..1501)
                     /locus_tag="PLY81_05735"
     CDS             complement(854..1501)
                     /locus_tag="PLY81_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNF29761.1"
                     /translation="MGLNNIGKLFMPKNKIFYELFEGVAARVSEMGYKLKEMVNEPDA
                     DKRASILSVIEDLEHKNDDATHLIFTELGRNFITPFDREDIHYLATALDDIADYIYAS
                     SKKISFYKVNPNDSGIQKLAELVLQGTTEISKAVKGLRDMKHLREMTEALVKVNSIEN
                     QADDVFDMSIEMLFNQENDFKEVIKKREIYQALETATDKCEDAANVIESIIIKYS"
     gene            1642..2952
                     /locus_tag="PLY81_05740"
     CDS             1642..2952
                     /locus_tag="PLY81_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNF29762.1"
                     /translation="MRKHLIKSLVFVIGLLYCTISNAQFLMDMVDTTKETGKGLLNIY
                     KKFDHLKISCYIQPQFQIAQSKGIPTFEGPDFNEEVNNRFLLRRSRLRFDYVHFEENN
                     TPGVQIAFQFDANERGFTLRDVWGRVFENKYKLFSFTTGMFARPFGYETNLSSSVRES
                     PERGRMNQLLMKSERDLGAMISLDARKKISGLQYLKVDLGLFNGQGINAKGDFDNSKD
                     VVARVALKPYKISKNITVSMGASALMGGLLQNTKYVYYTSLENGIKKILVDSAIDNKG
                     KNAPRKYIGTDVQLKIKNRIGFTELRAEFIAGKHAVLENSNTTPTSLVTDKNGYYIRN
                     FNGAYFYFIQNIFSQKHQLVVKYDWFDPNSRTSKKDIGKVGSNFNSADIKYNTLGLGY
                     INHVSNNIKLTLYYAKVWNEKTQLTGYTKDVKDDVFTCRLQFWF"
     gene            3216..4331
                     /gene="atpB"
                     /locus_tag="PLY81_05745"
     CDS             3216..4331
                     /gene="atpB"
                     /locus_tag="PLY81_05745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNF29763.1"
                     /translation="MVSRSMKSLLVLAFSLISLLFSNNTFAIGTADSTNSHHKAEKFD
                     VAKTILDHIRDDYSWHLWGHTSIPLPVILYSDKGLEVFSSAKLMDEHHSPIIYQGNYS
                     YKNIEGKIKVVNADGTVNAELNSKVWDFSFTKNVASLFISVFILLAVFLTVGAAYKKR
                     GASSAPKGLQSFMEPVVLFIRDEVAKPNIGHKYNTYLPFILTIFFLILINNLLGLVPF
                     FPGGANLSGNIAFTMTLAVFVFIVVNIKGNKSYWGHIFWMPGMHWGMKLFLAPIELVG
                     VFTKPISLTIRLFANMTAGHILVLSLICLIFIFENIFTSIVAVPFAAFIGLIECLVAF
                     IQAFIFAMLSSMYIGMAIEEHHHHGDNEPHYNESDEN"
     gene            4365..4589
                     /gene="atpE"
                     /locus_tag="PLY81_05750"
     CDS             4365..4589
                     /gene="atpE"
                     /locus_tag="PLY81_05750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNF29764.1"
                     /translation="MVLGAVVGSVAAIGAGIAAIGAGIGIGQIGKGAVEGISRQPEAA
                     NDIRSSMIIAAAFVEAVALFAVVVALLGNG"
     gene            4679..>4989
                     /locus_tag="PLY81_05755"
     CDS             4679..>4989
                     /locus_tag="PLY81_05755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNF29765.1"
                     /translation="MYLLEINPLVSPAIGLVFWNTVAFALLFFILRKFAWKPILNAIH
                     ERETGIENALSKAEKMKAEMKALANENEQLLQKAREERAVLIKEAKEQSDRMIADAKD
                     KA"
BASE COUNT         1745 a          745 c          819 g         1680 t
ORIGIN      
        1 tctaaggggg taactttggt tattctggtt cccattgttt ttacaatttt ccagccaccg
       61 ctcaatgtgc ctaaaccaat agcaaagaag ctacaaaaag gcacccaact atatttaata
      121 acaaattcat taaaagatag gctatggcct aaggtagatt cgtgataaat aattgcagca
      181 ccaataatgc ccattacctt ttgtgcatca ttaccaccat gccctaaact aaatgctgca
      241 ctagaagcaa gctgtagctt tttaaaccaa ctttctgctt tataatgatt acttcgttta
      301 aaaatatgta caattaaaat agtaattaaa aatgctataa acataccaat taaaggtgct
      361 ataaaaataa acaatactgt tggtaacact ttggcatagt taattacatc aattccgttt
      421 ttgctaaaag caccagcata agctaaagat gcaccaataa acccacctaa taatgtgtga
      481 gaggaggaag aaggaatacc aaaccaccag gtaattaaat tccaggttat tgcagccatt
      541 aaacctgcaa aaatcacttc taaggttata tagttttcat gtacactttt ggcgatagta
      601 ttaccaattt taaattctcc tatcaaatat tttgacacaa aaaaagcagc aaaattaaaa
      661 actgctgccc ataacactgc ctgaaacgga gtaagtactt tggttgaaac tattgtggct
      721 atagagtttg cagcatcatg aaagccatta atataatcaa aaataaaagc caatgcaatt
      781 ataacaatta gtaaaactgg aaaatccata gtaagtattt tgtgcttatt aatttaactt
      841 cttcaacgaa atattaagag tatttaataa ttatactttc aataacattg gctgcatctt
      901 cgcatttatc tgttgcagtt tctaaagcct ggtaaatttc tcttttctta attacttctt
      961 taaaatcatt ttcctgatta aataacattt caatactcat atcaaaaaca tcatcagctt
     1021 ggttttctat actatttact tttaccaatg cttctgtcat ttctctaaga tgtttcatat
     1081 ctcttaaccc tttaactgct ttactaattt ctgttgtgcc ttgtaaaaca agttcagcta
     1141 atttttgaat tccgctatca tttggattta ctttgtaaaa gctaattttt ttagaagaag
     1201 cataaatata atctgcaata tcgtctaatg cagtagctaa atagtgtatg tcttctctat
     1261 caaatggggt gatgaaattt ctacctaact cagtaaaaat taaatgggtg gcatcatcat
     1321 ttttgtgctc taaatcttca attacagata atatactagc ccttttatcg gcatctggct
     1381 cattaaccat ttctttaagc ttataaccca tttcgctaac tcttgctgct acaccttcaa
     1441 acaactcata aaaaattttg tttttaggca taaacaattt gccaatgtta tttaatccca
     1501 ttatttataa ttttattatt tgcaaagttg attattttac ttaatgatta atgcaacctg
     1561 taaattggta ataattcctt aacattggaa ataaatttgt atttttcttt acacattatt
     1621 tttacatttc taaacaaaaa catgagaaag caccttatca aaagccttgt atttgttatt
     1681 ggtttattgt attgcactat ttcaaatgct cagtttttga tggatatggt agatacaacc
     1741 aaagaaacag gtaaaggcct tttaaatata tataagaagt ttgaccattt aaaaattagt
     1801 tgctatatac aacctcaatt tcaaattgct caatctaaag ggatacctac atttgaagga
     1861 ccagatttta atgaagaagt taataataga tttttattaa gaagaagcag gctaagattt
     1921 gattatgtgc actttgaaga aaacaataca ccaggtgtgc aaattgcttt tcaatttgat
     1981 gcaaatgaaa gagggtttac tttaagagat gtgtggggca gagtttttga aaataagtat
     2041 aaactttttt catttactac aggcatgttt gcaagaccat ttggttacga aaccaattta
     2101 agcagtagtg ttagagagtc tccagagcgt ggaagaatga accagctttt aatgaaaagc
     2161 gaaagagatt taggtgcaat gattagtttg gatgcaagga aaaaaattag tgggctacaa
     2221 tatttaaaag tggatttagg cctatttaat ggccagggaa ttaatgctaa aggagatttt
     2281 gataatagta aagatgtagt ggcaagagtt gctttaaagc catataaaat ttcaaaaaac
     2341 ataactgtat caatgggagc ttctgcttta atgggtgggt tattacaaaa cacaaagtat
     2401 gtctattaca catcattaga gaacggaata aaaaaaatat tggttgattc agcaatagat
     2461 aataaaggaa aaaatgctcc aagaaaatat attggaacag atgtacagct taaaattaaa
     2521 aacagaattg gctttaccga attaagagca gagtttattg caggaaaaca tgctgtttta
     2581 gaaaatagca acaccacacc aaccagctta gtaactgata aaaatgggta ttatatcaga
     2641 aattttaatg gagcatattt ttatttcatt caaaatattt tctcacaaaa acaccaattg
     2701 gtagttaagt atgattggtt tgaccccaac tcaagaacct cgaaaaaaga tattggtaaa
     2761 gttggctcta actttaacag tgcagatata aaatataata cattaggatt gggatatata
     2821 aaccatgttt caaataatat aaaactaact ctgtattatg ccaaagtgtg gaatgaaaaa
     2881 acacagctaa caggttatac aaaagatgta aaagatgatg tgtttacctg cagattacaa
     2941 ttttggtttt aattttttgt gcattttttt aatgctaacc cctaattttt tttaaaaatt
     3001 tttcatttta tatgctatgt gttttaaaaa ccaggtataa atctataagt agtataaaaa
     3061 accaagtatt cttattaaag ttgaaactat tttagacaat gaagtactta ttaatgtata
     3121 aacaaattta tcttcaataa tttttaaaaa accaattcat aaaccaatct aatatcaaac
     3181 atacccctat ctttgccgca aatttcaaga aggatatggt gtcaagaagc atgaagtctt
     3241 tgctggtatt ggcttttagc cttataagcc ttttattttc aaataataca tttgcaatcg
     3301 gaactgcaga ttctacaaat agccatcata aagcagaaaa gtttgatgtg gctaaaacta
     3361 ttttagacca tattagagac gattatagct ggcatttatg ggggcacaca tctattcctt
     3421 tacctgtaat tctatattca gacaagggtt tagaggtttt ctcttctgca aagttgatgg
     3481 atgagcacca tagccctatc atttaccaag gaaattacag ttacaaaaat attgaaggta
     3541 aaattaaagt tgtaaatgcc gatggtacag ttaatgcaga attgaacagt aaagtgtggg
     3601 atttttcatt tacaaaaaat gtagcatcgt tatttatttc cgtatttatt ttattagcag
     3661 ttttcttaac tgttggtgca gcctataaaa aaagaggtgc ctcatcggca cctaaaggct
     3721 tacaatcatt tatggagcca gttgtattgt ttattagaga tgaagtagct aaaccaaata
     3781 ttggccataa atacaatact tatttaccat ttatattaac tattttcttt ttaatattaa
     3841 taaataactt gttaggatta gttcctttct ttccgggtgg tgcaaacctt agtggtaata
     3901 ttgcatttac aatgacgttg gcagtatttg tatttattgt ggtaaatatt aaaggtaata
     3961 aaagctattg ggggcatatt ttttggatgc caggaatgca ttggggaatg aaactatttt
     4021 tagcacctat tgaattggtt ggtgtattta caaagcctat ttcgctaact atacgattat
     4081 ttgccaacat gactgctggg catattttag tacttagctt aatttgctta atttttattt
     4141 tcgaaaatat ttttacatct atagtagcgg ttccttttgc agcatttatt ggtttaattg
     4201 aatgtttggt agcatttatc caagctttca tttttgccat gttaagttca atgtatattg
     4261 gaatggctat agaagaacat catcatcatg gagataatga gccacattat aatgagagtg
     4321 atgaaaatta aatttttatt ttttaacaat taaaacaaac aaacatggta ttaggtgcag
     4381 ttgtaggtag cgtagcagcc attggtgcag gtattgcagc aataggtgca ggtattggta
     4441 ttggccaaat tggtaaaggt gccgtagaag gtatttctcg ccaacctgaa gcagctaacg
     4501 acatccgttc aagcatgatc attgcagcag ctttcgtaga agcggtagca ttatttgctg
     4561 tggtggtagc ccttttaggt aacgggtaat ctacttttaa aagaaatcaa gctcaatgca
     4621 aagggcaaag agcttgattt ttaaaaacga tttcttattt agaatttaat tatacaatat
     4681 gtatttgtta gaaattaacc ctttagtgag tccggcaatt ggcttagtat tttggaatac
     4741 tgtggctttt gcattattgt tttttattct tcgcaagttt gcttggaaac caattttaaa
     4801 tgccattcac gaacgtgaaa caggcattga aaatgcatta agcaaagcag aaaaaatgaa
     4861 agccgaaatg aaggctttag ctaatgaaaa tgagcaattg ttacaaaaag ccagagaaga
     4921 aagagctgtt ttaattaaag aagcaaaaga acaatctgat agaatgattg ccgatgctaa
     4981 agataaagc
//
LOCUS       DAOQHP010000144         4989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9683_length_4989_cov_3.441427, whole genome shotgun sequence.
ACCESSION   DAOQHP010000144 DAOQHP010000000
VERSION     DAOQHP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9683_length_4989_cov_3.441427"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /locus_tag="PLY81_05760"
     CDS             complement(<1..2539)
                     /locus_tag="PLY81_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29766.1"
                     /translation="MKKFYLFFCLIVLAFATKTNGQTYYSLSSGDYTQDFNNWSGYAT
                     NWNGLPSEAIGSIPASTKRTVASNGTLAVVTTSPAIGYDVNPSTKLVFLSTGSSDNTS
                     AVACDLNLDFSGRNAGTLSFDADKINNGTGGRSATLRVYYSTDGTTWTELTGTDLPFT
                     VFNNVAKSAAISVSLPAALNNASTVKFRFYYHNGTGGATGSRPKITIDNLTVTSTPGG
                     GGGPATQLIYQNAPTTGIPNISLASFNVKAVAIDGISVDNTYTTDITISKASGPGNLT
                     GTLVKTPISGIATFNDLKFDAPGTYTLTASSGSLTQATTASIIVADASIITDHFRSNT
                     SLGNWSSISSWESSHDSATWMVATLAPDNNAASIVVQGIHELNVDASVSVSKLRVDGI
                     VNILASKTFTIVNDGDAGTKDFIITIGGTVLNQGTLTIGSGATWQVNDNASFIQNTTS
                     GISSALNSATLEDGSYFTYRGSSTLGITPSISGKTYSNLSLISESGVWRPSASGSGTF
                     TVKGHLFIGNDVRWNLNGFTGTLLFNGNNLQNFNIGDTAITLNNVTLNNTIGLVLQVA
                     GGKLNITNTLDVQDGTLTTGGMVVLKSDATRTARIAPITTGIISGNITVERFIPAKAT
                     RKWSLLSSPVTQSLANSWQQQIHITGAGTGGAICPTPTAHSNGFDATETNTSSIYTYN
                     AAGTPGSRWTAVASTYSDNLMSGKGFRVNVRGDRSLGCDLLNGVNMVPSAVTLKSTGA
                     ISNSDKNMGSFSITYPNVGINNYVLVGNPYPSALSFSGLQTANSSSINTNYAVYIPAS
                     AAGVYSYWDGVSFTGGSGYDDAKGNTIASGQAVFVQSLVAGDITLNYT"
     gene            complement(2752..>4989)
                     /locus_tag="PLY81_05765"
     CDS             complement(2752..>4989)
                     /locus_tag="PLY81_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF29767.1"
                     /translation="GTIDLATGNNTFGNQNNDITFPSGNEFVIKNDSVVVLIYKSTNL
                     IWYNKETDSLLVLKSVDDPYKIEGFTFTSSQDTLFYTTTEGMVYAFDVTKGKEYFSVK
                     LNAANDINFTMQPYYNKYNTKLYLKVNSSYIVALNTKTGAIEDSIYVTTARCMEFSSK
                     NIFVTTGLGRLRVFDYDSLLNIKTWQINGSESEALNIKLLPNEDKLLIQNSLLGICLY
                     DIKKDEFIYSKQDHKFGSYSMSVRKDGKEFVTAGLDGTIHVYETGTGNKKLTIRVPYK
                     KDPIFIDSNNYYMASKSSLNTINTYYNDNVYPYDQFDAQLNRPDIILKGLGRADSNLI
                     KSYYSAYKKRLKSQRLTDKPLGKQIHMPTIKFEDRYSLQTSTASNKLQLKVLCSDSLY
                     KIQSVFVLVNNTPILGVNGNNIAHLKTNSYLQNVTIPLARGNNKVKVYCTNSNGVSSL
                     KESFNVLSTFKDTLHNSYTYFVGIGVAKYKDSTRNLTYSVKDIRDLASDFKNNFKYIV
                     IDTLINETVTKENILALNKKLRKTRPNDRVIVAVTGHGLLNDSLDFYYATYDVNFNKP
                     DKRGLKYELLEALLDDVPAQEKVMFIDACHSGAFDKDEVLAVQRMNKNKNNSSVVKGI
                     ASRSSIILKTKKKSSTNSFELMQSAFSDLGGSNGTIIISAAGGMEFAYESPTWNNGVF
                     TYSIREGIFNKMADTNYFGNKNGEVSIQELSVYVNKRVSELTNNKQRPTSRKESLEFN
                     WIINY"
BASE COUNT         1592 a          865 c          750 g         1782 t
ORIGIN      
        1 cggtatagtt taatgtaata tcgcctgcta ctaaggattg tacaaatact gcctggccac
       61 tggcaatggt attgcctttg gcatcatcat agcctgaacc tcctgtaaaa ctaactccat
      121 cccaatagct ataaacacct gctgcacttg ctggtatata cactgcatag ttggtgttaa
      181 tgctgctact gttggctgtt tgtaaaccac taaagcttag ggcacttggg tatgggttac
      241 ctactaatac atagttgtta attcctacat taggataagt aatactaaag cttcccatat
      301 ttttatctga attgctgatt gctcctgttg atttaagcgt aacagcactt ggtaccatgt
      361 ttacaccatt taataaatcg caacctaaac ttctgtcacc tcttacattt accctaaagc
      421 cttttcctga cattaggtta tctgagtatg tacttgctac tgctgtccaa cggctgcccg
      481 gagtgcctgc tgcattgtaa gtataaatac tagaagtatt ggtttctgtt gcatcaaagc
      541 cattgctgtg ggctgtagga gtagggcaaa tagctcctcc tgtacctgct cctgtaatgt
      601 gtatttgttg ctgccaacta ttggctaaac tttgtgttac agggctactt aataaactcc
      661 attttctggt tgcttttgca ggtataaagc gttctactgt aatattacca ctaataatac
      721 ctgttgtaat aggagctata cgggctgttc tggttgcatc tgattttaat actaccatac
      781 ctcctgtagt taaagttcca tcttgaacat ccagagtatt agttatattt aatttaccac
      841 cggctacttg caacactaat cctattgtat tatttaaggt aacattattt aaggtaattg
      901 cagtatctcc aatattaaaa ttttgaagat tattaccatt aaacaataat gttccggtaa
      961 agccatttaa attccatcta acatcgttac ctataaataa atgtccttta actgtaaaag
     1021 tacctgagcc agaagctgaa ggacgccaaa cacccgactc acttattaga gaaagatttg
     1081 aataagtttt acctgagatt gaaggagtaa tccctaaggt ggaactacct ctatatgtaa
     1141 agtatgaacc atcctctaaa gtggcactgt ttaatgcact ggaaataccg gaagttgtat
     1201 tttgaataaa agaagcatta tcatttacct gccaagttgc tccactacca atagttaatg
     1261 ttccttgatt taaaactgtt cctccaattg taattataaa atcttttgtt cctgcatcgc
     1321 catcattgac aatagtaaat gtttttgatg ctaagatatt tacaatacca tctactcgta
     1381 gtttacttac acttacagaa gcatctacat ttaattcatg aataccttga actacgatag
     1441 aagctgcatt attatctggt gctaaagtag ctaccatcca ggttgcgcta tcgtggctgc
     1501 tttcccaaga tgaaatacta ctccaatttc caagcgatgt attggatcta aaatggtcgg
     1561 taataattga tgcatctgca acaataatag atgcagtggt tgcctgtgtt aaagagccag
     1621 acgaagcagt taatgtataa gtacctggtg catcaaattt taaatcgtta aaagtagcaa
     1681 ttccgctaat aggtgttttt actaaagtac cagttaaatt acctgggcca gaagctttag
     1741 aaatagtaat atctgttgta taagtattat caacacttat tccatcaatt gccactgcct
     1801 ttacattaaa actagctaaa gaaatattag gaataccggt agttggtgca ttttgatata
     1861 taagctgagt agcaggacca ccacctccac ccggtgtaga agttacagtt aagttatcta
     1921 tagttatttt tggtcttgac ccagttgcac ctcctgtacc attatgataa taaaacctaa
     1981 atttcacagt actagcatta tttagagcag caggcaaact aactgaaata gctgcagatt
     2041 ttgcaacatt attaaatact gtaaagggta aatcagttcc tgttaattca gtccaggttg
     2101 taccatctgt agaataataa actcttagtg tagcacttct accaccagtt ccattgttaa
     2161 ttttatcggc atcaaacgaa agtgttccag catttctacc agaaaaatct aaattcaaat
     2221 cacaagcaac tgcagatgta ttatctgaac tacctgtaga taaaaaaacg agttttgtag
     2281 aagggttaac atcataacca attgctggac tagtagtaac tactgctaaa gtgccattgg
     2341 aagctactgt tcttttagtt gaagcaggta tagagccaat tgcctctgag ggtaagccat
     2401 tccaattagt tgcataacca ctccaattat taaagtcttg tgtataatct ccagatgaca
     2461 gtgagtagta ggtttggcca ttagtttttg tagcaaaagc taaaacaata aggcaaaaaa
     2521 ataagtaaaa ttttttcata atgttccttt tagtttttga aataatagtt attgttcaaa
     2581 agtataacta taaagtaagg ggggtgcaaa tttaactatt aatatattat taagttttaa
     2641 ttattattgt tttttattaa cactgaatta aataataaat acttagtaag aattaagaga
     2701 aaatttttat tagtaaaatt attttttagt tatagcatct tgttcaccaa attaataatt
     2761 aattatccag ttaaattcta aactttcttt tctagatgtt ggtctttgtt tgttatttgt
     2821 taactcacta actcgtttat ttacataaac agaaagttct tgaatactta cttcgccatt
     2881 tttatttcca aaataatttg tatcagccat tttattaaaa ataccttctc ttatactata
     2941 agtaaaaaca ccattgttcc aagtggggct ttcataagca aactccatac ctcctgcagc
     3001 agatataata attgtgccat tgcttccacc taaatcgcta aatgcagatt gcattaattc
     3061 aaagctatta gttgaggatt tctttttggt ttttaaaata atagaacttc tacttgcaat
     3121 accttttaca acagatgaat tgtttttatt cttattcatt ctttgaacag ccaatacttc
     3181 atctttatca aatgcaccac tatggcaagc atcaataaac atcacttttt cttgtgctgg
     3241 cacatcatct aaaagagctt ctaacaattc atacttcagc cctctcttat ctggtttgtt
     3301 gaagtttaca tcataagtgg cataataaaa atctaacgaa tcatttagca atccatgacc
     3361 tgttacagct acaataactc tatcattggg tcttgttttt ctaagctttt tattcaatgc
     3421 taaaatattt tcttttgtaa ctgtttcatt tattaaagta tctattacaa tatatttaaa
     3481 attattttta aagtcagatg ctaagtctct tatatctttt acagagtagg ttaagtttct
     3541 agtactgtct ttgtattttg caacgcctat gccaacaaag taggtgtagg agttatgaag
     3601 agtatcttta aaagttgata atacattaaa tgattctttt aaactactaa cgccattgct
     3661 attggtacaa tatactttca ccttattatt accacgagct aatgggatag ttacattttg
     3721 caaataacta ttggttttta aatgtgcaat attattacca tttacaccca aaattggagt
     3781 gttattaact agtacaaata ctgactgtat tttgtacaaa ctatctgaac ataaaacttt
     3841 taactgcaat ttgttgctag ctgtagatgt ttgtaaacta tatctatctt caaattttat
     3901 agtaggcatg tgtatttgtt tgcctaaagg tttatcagtt aagcgttgag atttaagtct
     3961 ttttttatat gcagagtaat aagattttat taaattagaa tctgctctac ctaagccctt
     4021 taaaataata tctggcctgt ttaattgggc atcaaattga tcgtacggat acacgttatc
     4081 attataataa gtattaatag tgtttagtga acttttactt gccatataat aattattgct
     4141 atcaataaaa atgggatctt ttttatatgg tacacgtatg gtaagttttt tattgccagt
     4201 tccagtttca taaacatgaa ttgttccatc taaacctgca gttacaaatt cttttccatc
     4261 ttttctcaca ctcattgaat aggaaccaaa tttgtgatct tgtttgctat aaataaattc
     4321 gtctttttta atatcataca agcaaattcc taaaagagaa ttttgtatta ataatttatc
     4381 ctcattaggc aacaatttaa tatttaaagc ttcagattca gaaccattaa tttgccaggt
     4441 ttttatattc aacaggctat cgtaatcaaa aactcttaac ctaccaagcc ctgttgtaac
     4501 aaaaatattt ttagaagaaa attccataca ccttgcagtt gttacataaa tagagtcttc
     4561 aattgcacca gtttttgtat taagtgcaac aatgtagctt gagtttactt ttaaatacag
     4621 ctttgtatta tacttattat aatatggttg catggtaaaa ttgatatcgt ttgctgcatt
     4681 tagtttaacg gaaaaatatt ctttcccttt agttacatca aaagcataaa ccataccttc
     4741 tgtagtagta taaaacaaag tgtcttgtga ggaagtgaaa gtaaatcctt cgattttata
     4801 tgggtcatct accgacttaa gaactaataa actatccgtt tctttattat accaaattaa
     4861 atttgtgctt ttataaatta gtacaactac actatcattt tttattacaa actcatttcc
     4921 acttgggaaa gtaatatcgt tattttgatt accaaaagta ttatttcctg ttgctaaatc
     4981 tattgtacc
//
LOCUS       DAOQHP010000145         4932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9851_length_4932_cov_3.483494, whole genome shotgun sequence.
ACCESSION   DAOQHP010000145 DAOQHP010000000
VERSION     DAOQHP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9851_length_4932_cov_3.483494"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLY81_05770"
     CDS             <1..967
                     /locus_tag="PLY81_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNF29768.1"
                     /translation="THALIEENVQFNNLGFAVVDEQHRFGVAQRAALWKKSVIPPHIL
                     VMTATPIPRTLAMTAYGDLDYSIIDELPKGRQAITTVHRNEMQRMKVMEFIRDEVTKG
                     RQAYIIYPLIEESEKLSYEDLMQGYQETKSYFPEPKYSISVLHGKMPIAQKETNMQRF
                     VEGTTQIMVSTTVIEVGVNVPNATVMVIESAEKFGLSQLHQLRGRVGRGSEKSFCILL
                     TGSKVSTEALERIHTMCTTSNGFIIAEKDLELRGPGDVDGVRQSGALNFKLASIVNDR
                     ELLEKAKQMAEKVLTDDVELKLANNLPLKNYLNQQKGKTNWSKIS"
     gene            1088..4735
                     /locus_tag="PLY81_05775"
     CDS             1088..4735
                     /locus_tag="PLY81_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF29769.1"
                     /translation="MKLFCKILFIVVLNCITTLLYAQNYLEFVENKGQWDNKINYKGE
                     IANGAIALQKTGYRFMLHNNDDLNNRSAYFHGHTQPSQNKNKEQENFIVRSHVYEVKF
                     LNANSNPTIIPEKQIESYNNYYIDNDKSKWASGCKIYNTITYKNIYPNIDVRYYTNEN
                     NLKYDFIVREGGNVNDIILYFEGADKLKIKKGNLIIQTSVQDVKEMAPYSYVINNKGK
                     EEVKCSFDLRGNIVRFKIHEPIDKNATLVIDPTLIFSTFTGSTADNWGYTATYDGSGN
                     FYAGGIVLSTGFPVSNGAFQSTYQGGINTGEGSGFDVGIMKFNSTGSNRIYATYLGGS
                     SSNEQPHSLVVDKNNNLIVAGRTSSVNTFPTTLPTFGAGGGWDIFLTKFNATGTAVIN
                     SIRIGGTGNDGVNIKPKYPTGSGNGAITIRRNYGDDARSEVIVDQNDNIYLASCTQSA
                     DYPTTVGAAQTVGGSTPNGGIYQDGVIIKTSPDLSSVFFSTLFGGNGDDAAFVLALNP
                     SNNNIYFAGGTASTDLLGDKTNVLFSSNQGGGSNVDGFVSILKNDGSQFIKTTYMGTN
                     GIDIVFGIQFDKFSYPYIMGITTGNWPVIAANYSQTNGKQFISKLQPDLSGFIFSTKF
                     GTNQSSPNISPVAFLVDRCENIYVSGWGGGINGDPSANYVPNQSTVGLPVTADAIKSN
                     SDGSDLYFFVLEKNAQSLLYGSFFGQNDNAASIGEHVDGGTSRFDRNGVIYQSVCANC
                     GGGATFPTTPGTWSPNNGTGGQRCNLAAIKIAFNFAGVGSSVQSSVNGVKDTSGCVPL
                     TVTFKDTIANATKYIWSFGDGSPDATTTTPIVQHLYTAIGDYNVRLVSIDSNTCNIAD
                     TSYTILRVRNDDAFPSFTVTRLLPCTSLNYSFTNTSNFPSGKPFKSNSFTWDFGDGTT
                     QTAGGQTVTHSYAAAGTYNVKLILNDTNYCNSPDTAFESLKVAPLVVAKFETNTAGCI
                     PYNATFKNTSTAGTDFIWDFGDGSPISTATNPTHNYTTAGNFTIKLKAIDNGTCNKID
                     STSFTITVSPNPNSSFDFNPKPPQINTPVTFINNSVGGNKYSWHFGDGDSLVTNNINA
                     SVLHQYNETKLFNACLIVSNSYNCKDTSCNPIQARVVPKVGVPNAFTPNGDGINDKIF
                     VQGFGIQKMTWRIYNRWGKLLFTGISLGRGWDGKFNGEIQPQDVYNYVLDVKFSDGTT
                     QVIKGDFTLLK"
     gene            4754..>4932
                     /locus_tag="PLY81_05780"
     CDS             4754..>4932
                     /locus_tag="PLY81_05780"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF29770.1"
                     /translation="MSKYFKILFFLFTTFVVQKSHAQDLHFSQYFNAPLLINPANTGF
                     NPDFDYRVGGNYRNQ"
BASE COUNT         1723 a          719 c          901 g         1589 t
ORIGIN      
        1 tacgcatgcc ttaattgaag agaatgttca gtttaacaat ttaggatttg cagttgttga
       61 tgagcaacat cgctttggag ttgcacaacg tgctgcactt tggaaaaagt ctgttatacc
      121 acctcacatt ttggtaatga ctgcaacacc catacccaga acattagcta tgacagctta
      181 tggcgactta gattatagta ttatagatga attgcccaaa ggaagacaag ctataacaac
      241 tgtgcacaga aatgaaatgc aaagaatgaa agtgatggag tttattagag acgaagtaac
      301 aaaaggaaga caagcataca ttatctatcc attaattgaa gaaagtgaga aattaagtta
      361 tgaagatttg atgcaaggct atcaggaaac caaaagttat tttcctgaac caaaatattc
      421 catcagtgtt ttacatggta aaatgcctat tgcacaaaaa gaaacaaata tgcagagatt
      481 tgtagaagga accactcaaa taatggttag tactactgta atagaggtag gcgtaaatgt
      541 accaaatgca actgtaatgg ttattgaaag tgccgaaaaa tttgggttat ctcagctaca
      601 tcaactaaga ggtagagttg gaagaggtag tgaaaaaagt ttttgtattt tattaacagg
      661 tagcaaagta agcacagaag ccttagaacg tatccataca atgtgtacta ctagtaatgg
      721 ttttataatt gcagaaaaag atttagaatt aagaggccca ggagatgtag atggcgtaag
      781 acaaagtggt gctttaaact ttaaacttgc atctattgta aatgatagag aattattaga
      841 aaaggcaaaa caaatggccg aaaaagtatt aacagatgat gtggaattaa aattggcaaa
      901 taatttgcca ttaaaaaatt atttaaacca acaaaaaggc aaaaccaatt ggagtaaaat
      961 ttcctaacta agtaaatact aaaaagtcgt taaggaaatt ggtataaggt ttatatgtat
     1021 ttttaccaca attgataaag taaagtttgg atattatttt taaacaggaa ataataaatt
     1081 ttaagaattg aagctatttt gtaagatatt atttatagta gtacttaatt gtatcacaac
     1141 ccttttatat gctcaaaatt atctagagtt tgtagaaaat aaagggcaat gggataacaa
     1201 aataaactat aaaggagaaa tagcaaacgg tgccattgca ttgcaaaaaa ctggttatcg
     1261 ttttatgtta cataataacg atgatttaaa taaccgttct gcctactttc atggtcatac
     1321 acaaccatct caaaataaaa ataaagagca agaaaatttt attgtaaggt ctcatgtata
     1381 tgaggttaag tttttaaatg ccaattcaaa ccctacaatc ataccagaaa agcaaattga
     1441 aagttataat aactattata tagataatga taaaagcaaa tgggcatctg gttgtaaaat
     1501 atataataca attacctata aaaatattta tccaaacatt gatgttagat attataccaa
     1561 cgaaaataat ttaaaatacg attttattgt aagagaagga ggtaatgtaa acgatattat
     1621 tttatatttt gaaggggctg ataaattaaa aattaaaaaa ggaaacctca tcatccaaac
     1681 atctgttcaa gatgtaaaag aaatggctcc atacagttat gttataaata ataaaggcaa
     1741 agaagaggtt aaatgcagct ttgatttacg aggaaatatt gtgcgtttta aaattcacga
     1801 accaattgat aaaaatgcaa ctttagtaat tgaccccact ttaattttct ctacttttac
     1861 tggtagcaca gcagataatt ggggctatac tgcaacctac gatggtagtg gaaattttta
     1921 tgcaggagga attgttttat ctactggttt ccctgttagc aatggtgctt tccaatcaac
     1981 ttatcaagga ggtataaata caggtgaggg ctctggtttt gatgtaggaa ttatgaaatt
     2041 taattcaaca ggctctaaca gaatttatgc tacttattta ggaggcagtt cttctaacga
     2101 acaacctcat agtttagtag tagataaaaa caataattta attgttgcag gtagaactag
     2161 tagtgtaaat actttcccta ctactttacc aacttttggt gcaggtggtg gttgggatat
     2221 ttttctaaca aaatttaatg caactggtac agcagtaatt aatagtataa gaattggtgg
     2281 cactggaaat gatggtgtaa acattaaacc aaaataccca acaggttcag gaaatggtgc
     2341 aattactatt agaagaaatt atggagatga tgcaagaagt gaagtaattg ttgaccaaaa
     2401 cgataatata tatttagcaa gttgtacaca atctgcagat tatcctacaa ctgttggtgc
     2461 agcccaaaca gttggtggta gtacccccaa tggtggaatt tatcaagatg gtgttattat
     2521 caaaacttcg ccagatttaa gctctgtatt ttttagtact ttatttggtg gcaatggaga
     2581 tgatgctgct tttgtattag ctcttaatcc atctaataac aatatttatt ttgcaggagg
     2641 tactgcaagt acagatttgc taggcgataa aaccaatgta ctttttagtt ctaatcaagg
     2701 tggtggcagt aatgtggatg gctttgtttc tattttaaaa aatgatggct cacagtttat
     2761 taaaacaact tatatgggta ccaatggaat tgatatagtt tttggtattc agtttgataa
     2821 gtttagctac ccttatataa tgggtattac aacgggcaac tggcctgtaa ttgcagcaaa
     2881 ctatagccaa acaaatggta agcaatttat ttcaaaactt caacccgatt tatctggttt
     2941 tattttttca acaaaatttg gtactaatca aagtagtcct aatatttctc ctgttgcttt
     3001 tttagtggat cggtgtgaaa atatttatgt ttctggctgg ggtggtggca ttaatggaga
     3061 tccttctgcc aattatgtac ctaaccaatc aactgtaggt ttacctgtaa ctgcagatgc
     3121 tataaaatct aacagcgatg gtagcgattt atactttttt gtgttagaaa aaaatgcaca
     3181 aagtttatta tatggtagtt tttttggaca aaatgataat gctgcttcta ttggcgaaca
     3241 tgtagatggt ggaacgagca ggtttgatag aaatggcgta atttatcaat cggtttgtgc
     3301 caattgtggc ggtggtgcta cttttcctac tacacctggt acttggtctc ctaacaatgg
     3361 tacaggtggg caacgttgta atttagctgc cattaaaata gcatttaatt ttgctggtgt
     3421 aggcagttct gttcaatcat ctgttaatgg tgttaaagat acttctggtt gtgtgccttt
     3481 aacagtaact tttaaagata caattgctaa tgccactaaa tatatttgga gttttggaga
     3541 tggcagccca gatgctacaa ctaccacacc aattgtgcag catttatata cagctattgg
     3601 agattataat gttagattgg tttctataga ttctaatact tgcaatattg cagacacatc
     3661 ttatacaatt ttaagagtaa gaaatgatga tgcctttcct agctttacag taacccgttt
     3721 actaccctgt acaagtttaa attatagttt taccaatact tccaactttc caagtggaaa
     3781 gccttttaaa agtaatagtt ttacttggga ttttggtgat ggtacaacac aaacagcagg
     3841 tgggcaaaca gtaacgcatt cttatgctgc cgctggtact tataatgtaa agctaatttt
     3901 aaacgatact aactattgta attctccaga tactgccttt gaaagtttaa aagttgcacc
     3961 tttagttgta gcaaagtttg aaacaaacac tgcaggttgt atcccttaca atgcaacatt
     4021 taaaaacaca tcaacagctg gaactgattt tatttgggat tttggtgatg gctcacctat
     4081 ttcaactgct acaaatccaa cgcataatta tactactgct ggtaatttta caattaaatt
     4141 aaaagccata gataatggta cttgtaataa aatagatagc acttcattta ctataactgt
     4201 tagcccaaat cctaattcat ccttcgactt taatcctaaa cctcctcaaa taaatacacc
     4261 agttacgttt atcaataatt ctgttggtgg caataaatac tcttggcatt ttggcgatgg
     4321 agatagcttg gttactaata atataaacgc tagtgtttta caccaatata atgaaacaaa
     4381 attatttaat gcttgtttaa tagtgagcaa tagctataat tgtaaagata caagctgcaa
     4441 ccctattcag gcaagagtag tgccaaaagt aggcgttcca aatgcattta caccaaatgg
     4501 cgatggtata aatgataaaa tatttgtgca aggatttggc atccaaaaaa tgacttggag
     4561 aatttataat cgttggggga aattgctttt tacaggcatt agtttaggta gaggttggga
     4621 tggcaaattt aatggtgaaa tacagccaca agatgtttac aactatgtgc ttgatgtaaa
     4681 gtttagcgat ggtacaacac aggttataaa aggcgatttt actttattaa aataatcttt
     4741 gaggttcaat taaatgtcta aatattttaa aatattattt tttcttttta caacatttgt
     4801 tgtacaaaaa tctcatgctc aggatttaca tttttctcaa tactttaatg ccccattact
     4861 tataaatcct gccaatactg gctttaatcc agactttgat tatagagttg gaggtaatta
     4921 tcgcaaccaa tg
//
LOCUS       DAOQHP010000146         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_9904_length_4910_cov_4.203296, whole genome shotgun sequence.
ACCESSION   DAOQHP010000146 DAOQHP010000000
VERSION     DAOQHP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9904_length_4910_cov_4.203296"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3232
                     /locus_tag="PLY81_05785"
     CDS             <1..3232
                     /locus_tag="PLY81_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29771.1"
                     /translation="ADFNGQHVTIQSDATGTGMIGQVSGFIEDANNVTVERYISASGN
                     RAYRLLTPSVTTSNYIRDNWQEGTNNPDVATNNNPNPGYGTHITGAGGSSNGFDPTQN
                     NQVSLYKLVTNAWVAVSNTNSTILEADKAYLLFVRGSRDNINTINTLTGSSNTTLRAT
                     GTIKQGGQTFSSLPASSFSLVTNPYPSPLNWGSVYTANSSNFENYVYIWDPNIGVRGG
                     YVAVEDDGTTSVASALTQNLQSGQAFFVRTKSGIASLAITEADKASVNNLNVYKTSET
                     AEKLNIQLRFTKDGKDVLADGVLARFASNYNNVVDGNDAIQITNWDEDIALVRNSSNL
                     SIESRNTISAADTLFLKMANLRVAQGTYNWIMAPTNFNNNNLQAYLEDAFTGTRKSIS
                     LIDTNRIAFTVSNNVASTASNRFRIVFAERKIYYSKSNNSNANELSSWSSTTDGNGTT
                     PSSFNELANFVVQQGHTLQLADTLHLTEGTLTIKPNAEINNNGLLSVAYNIENNGTVS
                     GNGLLQLNGSIKQYFTGYGTYSKVELDNSNGAEIFAEAQVNITTAYKPTNGILTTNNN
                     LVFKSSAQKTASISSGNTTGNYINGNITLETYVPAKAVRKWSFISSPLTQSIANGWQQ
                     QIHITGAGTGGSVCPTLLPHNNGFDATLTNASNIYTYDASKASGQRWAALPTTTTDFT
                     NAGKAFRINIRGNRNLGCSLLDGTINAPSEVTVTTTGLLNNEAKNAGNFSIEFANNGV
                     NNYVLIGNPYPSAISFSSLYSQNSTSINNNYALYIPTNNAGVYTYWDGLTNTFTGGVG
                     YDDITGNNIATAQAVFVQSAVAGNIVLHFSENIKTEENATAYFKNNSVKEKIKINYLN
                     SSGNKIDETVVRFISDNAVTNNEVGRLDIPSINTGTFIATIKNNTQLAVHSRALNNLI
                     SDEVWLNIGATQTGNYTLHFSDYQTLEDADIFLEDHYLNTKQNIKLNDNYSFSVDVNN
                     AATKGSARFSLVFIRTAPSEVVYNQIKMYPNPADKQVSLLLPKGIDNGINYQIKLTDI
                     TGKIVIQRKAKGGKEVIDIASLTAGIYIVEITDSKGKRVTEKLVKN"
     assembly_gap    3395..3494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3930..>4910)
                     /gene="der"
                     /locus_tag="PLY81_05790"
     CDS             complement(3930..>4910)
                     /gene="der"
                     /locus_tag="PLY81_05790"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNF29772.1"
                     /translation="KPVYLTVNKVDNHSRELDATEFYSLGFEKIYTLSSISGTGTGEL
                     LDAITEKIELTDIADNNTENELPKFAIIGQPNVGKSSLLNALIGQERTIVSNIAGTTR
                     DTIHTHYNLFNKEFILIDTAGIRKKSKEKDDLEFYSIIRAIKAMDEADICLLLLDAEK
                     GITAQDVAIFSLAARKGKGIAILVNKWDLVEKETNTARDYEKKLKEKIAPFTDVPILF
                     ISVKDKTRIFKAIEIALEVYNNKQQKISTSKLNEVMLKVVEANKAPVVRGHIVKIKFI
                     TQLPTHVPSFAFFTNFPNDIKTPYKNFLENQLRAKFNFKGVPIRIFFRKK"
BASE COUNT         1743 a          724 c          815 g         1528 t
ORIGIN      
        1 tgcagatttt aatggtcagc atgtaactat acaatccgat gcaacaggta caggaatgat
       61 aggacaagta agtggtttta tagaagatgc aaataacgtt acagtagaaa gatacattag
      121 tgcatctggc aacagggctt atcgtttatt aacaccaagt gtaacaacca gtaattatat
      181 tagagataat tggcaagagg gaaccaataa tccagatgtt gcaaccaaca ataatcctaa
      241 tccaggttat ggtacacata ttacaggagc aggaggttca agcaatggct ttgaccctac
      301 acaaaataac caggtttctt tatacaaatt agtaacaaat gcttgggtag cagtaagcaa
      361 tacaaacagt acaattttag aagcagataa agcttattta ctttttgtac gaggtagtag
      421 agataatatt aatactatta ataccttaac aggcagtagt aacactactt taagagcaac
      481 aggcaccata aagcaaggtg gccaaacatt tagtagctta ccagcttcgt cttttagttt
      541 agtaaccaat ccttatccat ctcctttaaa ttggggttca gtttatactg ctaacagcag
      601 caactttgaa aattatgtgt atatatggga tcctaatatt ggggtaagag gtggttatgt
      661 tgctgtagaa gatgatggaa caaccagtgt agcatctgcc ctaacacaaa acctacaaag
      721 cggacaagca ttttttgttc gtactaaatc aggtattgcc agcttagcca taacagaagc
      781 agataaagcc agtgtaaata atttgaatgt atataaaact tctgaaactg cagaaaaatt
      841 aaacattcag ttgcgtttta ctaaagatgg taaagatgta ttggctgatg gtgttttagc
      901 aagattcgca agtaattaca acaatgttgt tgatggtaac gatgccattc aaattactaa
      961 ttgggatgaa gatatagcat tggtaagaaa tagttctaat cttagtatag aaagcagaaa
     1021 tactatttct gcagcggata ctttgttttt aaaaatggcc aatttacgtg tagcacaagg
     1081 aacttacaat tggataatgg ctccaactaa ttttaacaac aacaatctgc aagcctattt
     1141 agaagatgca tttacaggaa ccagaaaatc cattagctta atagatacta accgtatagc
     1201 atttacagtt tctaacaatg tagcatctac tgcaagcaat agatttagaa ttgtttttgc
     1261 agaaaggaaa atatattata gcaaatctaa taatagcaat gcaaatgagc taagtagttg
     1321 gagtagtaca acagatggta atggtacaac accttcttct tttaatgagt tagcaaactt
     1381 tgttgtacag caaggacata cactacaact tgcagatacc ttacatttaa ctgaaggtac
     1441 attaaccatt aaacccaatg cagaaattaa taataatggt ttactaagtg ttgcttataa
     1501 tatagaaaat aatggaactg tttcgggtaa tggtttatta caattaaatg gtagtattaa
     1561 acagtatttt actggctatg gcacttacag caaagtagaa ttagataata gcaacggtgc
     1621 cgaaattttt gcagaagccc aagtaaatat tactactgca tataaaccaa ctaatggcat
     1681 acttacaacc aataataatt tagtatttaa aagctctgca caaaaaacag caagtataag
     1741 ttcaggaaat actactggaa attatatcaa cggaaatata acactagaaa cttatgtacc
     1801 tgctaaagct gtaagaaaat ggagttttat atcaagtcct ttaacacaaa gtatagcaaa
     1861 tggttggcaa caacaaatac atataacagg tgcaggaaca ggtggttctg tttgccctac
     1921 tttattacca cataataatg gttttgatgc tacattaacc aatgcatcta atatatatac
     1981 ttatgatgca tctaaagcaa gtggccaaag atgggctgca ttaccaacta caactacaga
     2041 ttttaccaat gcaggcaaag cctttaggat aaatattaga ggtaatagaa acttaggctg
     2101 cagtttatta gatggaacaa taaatgcacc atcagaagta actgtaacta caaccggttt
     2161 gttaaataac gaagcaaaaa atgccggaaa ctttagtatt gaatttgcaa acaatggggt
     2221 aaataactat gtgttaattg gtaatcctta cccaagtgca attagcttta gtagtttata
     2281 tagccaaaat tctaccagca taaacaataa ttatgcacta tatattccta ccaataatgc
     2341 aggagtttac acttattggg atggcttaac aaatacattt acaggtggtg taggttatga
     2401 tgatataaca ggtaataata tagctactgc tcaagcagta tttgtacaat cggcagtggc
     2461 aggtaatata gttttacatt tttctgagaa tataaaaaca gaagaaaatg caacagcata
     2521 tttcaaaaac aattcagtaa aagaaaaaat taaaataaat tatttgaata gcagtggtaa
     2581 taaaatagat gaaacagttg tacgatttat ttcagataat gcagttacta ataatgaagt
     2641 tggaaggtta gatatacctt ctattaatac aggaacattt attgcaacaa ttaaaaataa
     2701 tacacagctt gcagtacatt ccagagcatt aaataattta attagcgatg aggtttggtt
     2761 aaatattggt gcaacccaaa caggcaatta tacattacac tttagcgatt atcagacatt
     2821 agaagatgca gatatttttt tagaagatca ttatttaaac accaaacaaa atatcaaatt
     2881 aaacgataat tatagtttta gtgtagatgt taataatgct gctacaaaag gctctgcaag
     2941 gtttagttta gtattcatca gaacagcacc cagcgaagtg gtgtacaacc aaattaaaat
     3001 gtatcccaat ccagcagata aacaagtgag tttattgttg ccaaaaggga tagataatgg
     3061 tataaattat caaataaaac taacagatat aacaggcaaa atagtgatac aacgcaaagc
     3121 aaaaggaggt aaagaggtaa ttgatattgc atcactaaca gcagggattt atattgtaga
     3181 aataacagac agtaaaggaa aaagagtaac agaaaaattg gtaaagaatt aagagttaag
     3241 aattaagaat taagaattaa gaattatgag ttatgagtta agaattatga gttaagaatt
     3301 atgagttaag aatttagaat tatgaatgtc atcctgagtc tctttttgtc atcctgagcg
     3361 taagcgaagg atctaaaggt taagaacaat gagannnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnctcttt ttgtcatcct gagcgtaagc gaaggatcta aaggttaaga
     3541 acaatgagat tcctctcagg ctcggaatga caaacctttt tttatgtcac ttcgttcaac
     3601 atgacaattc ttttttttca tttcattcat aatgccacag tttaaacaaa agtaaagcac
     3661 cctataagca ggtaagccta cttaaaaaat gttcactata aattaatatt aataccatgc
     3721 cttgttggca ttggttggtt gttgttattg ttttaagggt tttaccagtt ttgttagcag
     3781 tagcttttta tttttaaaaa taaaagttgc tttgcaaaaa taataaacga taaagatagc
     3841 ttaaatgagt taataataaa aaagaattat ataaaaaaat acacaattac ccaattaatt
     3901 attcacaatt aaaaaggttt acatggctat tatttctttc taaagaaaat tctaattggt
     3961 actcctttaa aattaaattt tgccctaagc tggttttcta aaaaattttt atacggggtt
     4021 ttaatatcgt ttggaaagtt agtaaaaaat gcaaaggaag gtacatgggt tggtaattgt
     4081 gttataaact taatttttac aatatgcccc cgtaccactg gtgctttatt ggcctctacc
     4141 acttttagca ttacctcgtt tagtttagaa gtgctaattt tttgttgttt attgttatac
     4201 acttctaaag caatttctat ggctttaaaa attcgggttt tatcttttac agatataaat
     4261 aaaataggca catcggtaaa cggggctatt ttttctttta attttttttc ataatctctt
     4321 gcagtattag tttctttttc taccaaatcc catttattta ctaaaatagc tatgccttta
     4381 cctttacggg cagccaggct aaaaatggct acatcttgtg ccgttattcc tttttctgca
     4441 tctaaaagca ataaacaaat atctgcttcg tccattgctt taatagctct aatgatagag
     4501 taaaattcca ggtcgtcttt ttctttactt ttctttctaa tacctgcagt gtctattaaa
     4561 ataaattctt tattaaataa gttataatgt gtatgaatag tatctctggt agtgcctgca
     4621 atattgctaa caatggttct ctcctgccca attagtgcat ttagtaaaga tgatttgcca
     4681 acatttggtt gaccaataat ggcaaatttt ggcagttcat tttctgtatt attatctgct
     4741 atgtcggtta gttcaatttt ttctgtaatt gcatctaata attcgccagt gcctgtacca
     4801 ctaatgctgc taagagtata aattttttcg aaccctaaac tataaaattc ggtagcatct
     4861 aattctctgc tatggttatc tactttgttt acagtaagat aaactggttt
//
LOCUS       DAOQHP010000147         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10005_length_4879_cov_3.471808, whole genome shotgun sequence.
ACCESSION   DAOQHP010000147 DAOQHP010000000
VERSION     DAOQHP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10005_length_4879_cov_3.471808"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2341)
                     /locus_tag="PLY81_05795"
     CDS             complement(74..2341)
                     /locus_tag="PLY81_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNF29773.1"
                     /translation="MAHQANTDPEALSVAYNLNEEEEKKEILRRYRSLLKALRPKLKK
                     GNRQIVRNAFEMAADAHKTMRRKSGEPYIFHPIAVAMICVEEIGLGVRSTICALLHDT
                     VEDTDLTLEDIEREFGNEIAKIVDGLTKISTVFDNNTSQQAENLKKILLTLTDDPRVI
                     LIKLADRLQNMRTLEFMKREKQLKVSSETIWVYAPLAHRMGLYNIKTELEDLAMKYME
                     PEAYKDIAKRLAETKKERTKYINEFIKPLKEKLTNTGFNFEIYGRPKSIHSIWNKIKK
                     KDVTFNEVYDLFAIRIILNPPPEKEKEECWKVYSIITDEYNPNPERLRDWLSNPKSNG
                     YEALHTTVMGPQGKWVEIQIRTKRMNEIAEKGLAAHFKYKEGSNNEDRFDKWLGQIRE
                     ALSQDDTDSINFLKDFKTSFLAEEIYVYTPKGEVKMLPMGSTALDFAFAIHTAVGSNC
                     IGAKVHHKLVPISYKLKSGDQVEIITSSKQKPSEDWLNIVVTTKAKTKIKDALKEEKR
                     KIADDGKYVVQKKLEGLGAAWNTANIDELIVFYKLPSHLELFYKVAIKKIDLKELKEF
                     QVIGDRIEAPKTKIISKETITDLPTNKPSSKKDSELIIFGESSDKIQYNLAKCCNPIP
                     GDDVFGFVSSGKGLIIHRTNCPNATQLLANFGHRVVKTKWAKNKEISFLTGLKIIGMD
                     DVGVVHKITNIISGDLKINIAGLTIESSEGLFEGTIKVFVHDKEELDELVDRIKQLNG
                     IQKVDRFDMEEVANK"
     gene            complement(2451..>4879)
                     /gene="infB"
                     /locus_tag="PLY81_05800"
     CDS             complement(2451..>4879)
                     /gene="infB"
                     /locus_tag="PLY81_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNF29774.1"
                     /translation="TAKAKTTEPTKEQPSSKKEETAKVEQQEPKVEPAITEETNSIVK
                     PPDTASTIVENIEVEKLTGPKILGKITLPVDSDTRPKPISKVEQHKRKRIVVDKKPGS
                     NIQRVDFRKEGGGQTQTGNNNRGGNQQQQNRFGGKPGGGGGHPQRGGGRNAGNNRGKQ
                     DNRRVADKEIDAKEIQDKIKETQAKLAGSGGRGKSLKAKYRREKRQEAAEAAMEGSGL
                     DDNKLQVTEFVTVSELANLMDVSFTDVISKCMGLGLMVSINQRLDAEVIELVAGEFGF
                     EAEFTSIDDVEDEEEDDEDTPENLAPRSPIVTIMGHVDHGKTSLLDYIRNASVVAGEA
                     GGITQHIGAYEVTLANGKNITFLDTPGHEAFTAMRARGAKVTDIAVIVVAADDAVMPQ
                     TKEAISHAQAANVPMIFAVNKIDKDGANPQKIYEQLSQMNLLVESWGGKYQSQELSAK
                     KGLHINELLEKILLEAELLELKANANREATGTIIEASLDKGRGYVATILVQNGTLKQG
                     DIIVSGQYYGRIKAMFNERNQRVQEAPPSTPVVILGLNGAPQAGEKFKVYSDEAYAKD
                     IATKRAQIIREQGLRARKHITLDEIGRRLALGNFKELNIIIKGDVDGSVEALSDSLQK
                     LSTSEVAVNVVLKAVGQISESDVLLATASDAVLIGFNVRPSSQASKLAATEGVQIKQY
                     SIIYTAIEEIKSAMEGMLEPKVEEKEVATVEIREVFRFDKAIVAGCYVTEGKIKRDHK
                     IKVFRDGILVYPRQEGAYAELGSLKRFKDDVKEVNNNYECGLTVKGFTDIQAGDTVVA
                     YEEEEIKRTL"
BASE COUNT         1268 a          945 c          675 g         1991 t
ORIGIN      
        1 taagataggg agtaagaaac aaaattttaa ataagaaata tttactcttt ttatgatgta
       61 gcctgtatta acattatttg ttggctacct cttccatatc aaacctatct actttttgaa
      121 taccatttag ttgtttaatt ctatctacta attcatctag ttcttcttta tcgtgtacaa
      181 atacttttat ggttccttca aataaacctt cgctactttc tatagttaaa ccagcaatat
      241 ttatttttaa atcgccacta ataatattgg taattttatg taccacacca acatcatcca
      301 taccaataat ttttaagcca gttaaaaaag atatttcttt attttttgcc cattttgttt
      361 taactactct atgtccaaaa ttagctaata actgggtggc attagggcaa ttagttctgt
      421 gaataattaa acctttgcca gagcttacaa aaccaaaaac atcatcgcca ggaattgggt
      481 tacaacattt ggctaaatta tattgtattt tatcgctgct ttcgccaaaa ataatcagtt
      541 cactatcttt tttagaagat ggtttgtttg taggaagatc ggtaatggtt tctttagaaa
      601 taatttttgt ttttggagcc tcaattctat cgcctataac ctgaaactct tttagttctt
      661 ttaagtctat tttttttata gctactttat aaaataattc aaggtgagag ggtagtttat
      721 aaaatactat taactcatca atgttggctg tattccatgc tgcaccaagg ccttctaact
      781 ttttttgcac tacatacttt ccatcgtctg caatttttct tttttcttcc ttaagtgcgt
      841 ctttaatttt tgtttttgct tttgtagtta caacaatatt tagccaatct tctgatggtt
      901 tttgtttaga gcttgtaata atttctactt gatctccact tttaagttta taggaaattg
      961 gcactagttt atgatgaact ttagcaccaa tacagttact accaacagca gtatgaattg
     1021 caaatgcaaa gtctaatgct gtactgccca ttggtaacat ttttacttcg cctttaggag
     1081 tgtaaacata aatttcttcg gcaagaaatg atgttttaaa atcttttaaa aagtttatac
     1141 tatctgtatc gtcttgagaa agtgcttctc taatttgccc taaccattta tcaaatctat
     1201 cttcattatt gctgccttct ttgtatttaa aatgtgcagc taaccctttt tctgcaattt
     1261 cattcattct tttagttcgt atttgtatct ctacccattt tccttgaggg cccatcactg
     1321 ttgtatgcaa agcctcgtat ccattgcttt ttgggttgct tagccaatct cttaatcttt
     1381 ctgggttagg gttgtattca tctgtaataa tgctataaac tttccagcat tcttcttttt
     1441 ctttttctgg cggtggattt agaattattc taattgcaaa caaatcatat acttcattaa
     1501 atgttacatc tttctttttt attttattcc atattgagtg aatgcttttt ggcctaccat
     1561 atatttcaaa attaaatcct gtattagtta acttttcttt taatggtttt atgaattcat
     1621 tgatatattt tgttctttct tttttagttt ctgctaatct ttttgcaatg tctttatagg
     1681 cttctggctc catatatttc attgcaaggt cttctagttc ggttttaata ttatataagc
     1741 ccattctatg agcaagaggt gcatataccc aaatagtttc gctagatact tttaattgct
     1801 tttccctttt cataaattct agggttcgca tattttgaag cctgtctgct aattttatta
     1861 atattactct tgggtcatct gttaaggtta gaagaatttt ttttaaattt tctgcttgtt
     1921 gagaggtgtt gttatcaaat acagttgaaa tttttgttaa cccatccaca atttttgcta
     1981 tttcgtttcc aaattctctt tcaatatctt ctaaggttaa gtctgtgtct tctactgtat
     2041 catgcagtaa tgcacaaatg gtgcttctta ctcctaatcc aatttcttcc acacaaatca
     2101 tagcaactgc aataggatga aaaatataag gttcgccact ttttctacgc atagttttat
     2161 gggcatctgc tgccatttca aaagcattgc gtacaatttg cctattacct tttttaagtt
     2221 ttgggcgtaa agcttttaag aggcttctgt aacgccttaa tatttctttt ttttcttctt
     2281 cttcatttaa attatatgca acagagagtg cttctgggtc tgtatttgct tgatgggcca
     2341 tagtgtaacg aatatatgaa ttttttttac gtaaatattt tttaaggcta ttgttaaaac
     2401 aaaaaaagag gctgagtaat cagcctctta aatatttata ctatttatag ttatagtgtt
     2461 cttttaattt cctcttcttc gtaagcaaca acagtatcgc ctgcttgaat gtctgtaaat
     2521 ccttttacag ttaaaccgca ttcataattg ttgtttacct cttttacatc gtctttaaat
     2581 cgttttaagc ttcctaattc agcataagca ccttcttgtc ttggataaac taaaatgcca
     2641 tctctaaata ctttaatttt atggtctctc ttaatttttc cttctgttac ataacaacca
     2701 gcaacaatag ctttatcaaa tctaaatact tctcttatct caacagtagc aacttctttt
     2761 tcttctactt taggttctaa cataccttcc atagcactct taatttcctc aattgcagta
     2821 taaataatag agtattgttt tatttgtact ccttcggtag ctgctagttt acttgcttga
     2881 gaagatggac gaacattaaa cccaattaaa acagcatcag aagctgtagc taaaagtaca
     2941 tcgctttctg aaatttgacc aaccgctttt aaaactacat taactgctac ctcggatgtt
     3001 gaaagttttt gtaagctatc gcttaatgct tcaacggaac catccacgtc tcctttaata
     3061 ataatattta actctttaaa attacctaaa gcaagccttc tgcctatttc atctaaagta
     3121 atatgttttc ttgcacgcaa gccttgctct ctaattattt gagctctttt ggtggcaata
     3181 tcttttgcat aagcttcatc ggaataaact ttgaattttt ctcctgcttg tggtgcacca
     3241 tttaatccta atattacaac aggggtagat ggtggggctt cttgaacacg ttgattacgt
     3301 tcgttgaaca tggcttttat tctaccataa tattgaccag atacaataat atcgccttgt
     3361 tttaaagtac cgttttgaac aagaattgtt gcaacataac cacgtccttt atctaaagat
     3421 gcttcaataa ttgttcctgt agcttctcta tttgcattgg cttttagttc taataattct
     3481 gcttctaata atattttttc taaaagttca tttatatgaa gccctttttt tgcacttaat
     3541 tcctgactct gatatttacc tccccaactt tctactaata aattcatttg agataattgc
     3601 tcataaattt tttgaggatt tgctccatct ttatcaattt tatttacagc aaaaatcatt
     3661 ggtacattgg ctgcttgagc atggctgatt gcttctttag tttgaggcat tactgcatcg
     3721 tctgcagcaa ccacaataac tgcaatatcg gttacttttg cacctcttgc cctcatagct
     3781 gtaaaagctt cgtgacctgg tgtatctaaa aatgttatat tttttccgtt agctaatgtt
     3841 acctcataag caccaatatg ttgtgtaatt ccacctgctt ctcctgctac tacactagca
     3901 tttctgatat aatcaagtaa tgaagtttta ccatgatcta cgtgtcccat aatggttact
     3961 attggagacc ttggagctaa attttctggc gtatcttcat cgtcttcctc ttcatcttct
     4021 acatcatcaa tgctggtaaa ttcagcttca aaaccaaact ctccggcaac taattcaatc
     4081 acttcggcat ctaatctttg gtttattgat accattaagc ccaagcccat acatttactg
     4141 attacatctg taaagcttac atccattagg ttagctagtt cacttactgt aacaaactct
     4201 gtaacttgta atttattatc atctaatcca cttccttcca tggcagcttc tgcagcttct
     4261 tgcctctttt ctcttctgta ttttgctttt aggcttttac ctctacctcc actacctgca
     4321 agttttgctt gtgtttcttt aattttatcc tgaatttctt ttgcatcaat ttctttatct
     4381 gctactctac gattatcttg tttgcctcta ttatttccag catttcttcc tcctcctctt
     4441 tgtggatgac ctccaccacc tcctggtttt cctccaaatc tattttgctg ttgctggttt
     4501 ccaccacggt tattattacc tgtttgtgtt tgccctcctc cttcttttct aaaatctaca
     4561 cgttgtatat tgctacctgg ctttttatct acaactatac gtttacgttt atgctgttct
     4621 acttttgata ttggttttgg ccttgtatca ctatctacag gtaatgtaat tttgcctaat
     4681 atttttggcc ctgttaattt ctcaacttca atattttcta caattgtact tgctgtatcc
     4741 ggtggtttta caatgctgtt agtctcctct gtaatagcag gttccacttt tggttcttgc
     4801 tgttctactt ttgcagtttc ttctttttta cttgaaggtt gttctttggt tggttcagtt
     4861 gtttttgctt tggcagttt
//
LOCUS       DAOQHP010000148         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10008_length_4877_cov_3.701576, whole genome shotgun sequence.
ACCESSION   DAOQHP010000148 DAOQHP010000000
VERSION     DAOQHP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10008_length_4877_cov_3.701576"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLY81_05805"
     CDS             <1..208
                     /locus_tag="PLY81_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29775.1"
                     /translation="ADNHTKDSEDFCSPFCVCFCCRNLVLQQDEIKDLEVQPTIFYLA
                     SQSIYSTATIPTAFLNNIWQPPKV"
     gene            295..2490
                     /locus_tag="PLY81_05810"
     CDS             295..2490
                     /locus_tag="PLY81_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNF29776.1"
                     /translation="MKKIIALLLVVMVCISSNVKAQHTIVTKVFNHENDEVLQGVLIK
                     IAKTNIRTVTNENGVGVLKNVPKGNFKIEFSYTGFESASVQVTVPQAKNDTLIVALHN
                     HEEELEEVFVQSTRTSRTIKNSPTRVETISGEELDEKNNMRPANVSMLIHESTGMQLQ
                     QTSATTGNASIRVQGLDGRYTQLLKDGYPNFGNFANGLSVLEIPPLDLKQVEIIKGPA
                     STLYGGGAIAGVVNFISKTPKEKLEGNFILNQSNIGQSNIAGYLQQKKGKFGFTVLAA
                     YNYSKAYDVDKDDFSEIPKSNNYTIHPRLFFYPDSKTTITIGNNLTQSNSKGGDMQVI
                     KGNSSLGHVYFEKNTTTRNTVTLEADRKINSDSRFYLKQSFSTFNRNLQTNNHEFAGL
                     STNSYTDLSYTFNKNNHTIISGLNIIYDDFKQHKVTNAFLNSKSFTTGFYAQDTWDVS
                     EIIKIEGGLRVDNVTYKNVNFSKNQTFVLPRVSVLLNYSNHLSSRIGAGLGYKVPTIF
                     TEETEAMHYNNLQALDNSVTSERSVGGTIDVNYKNKIGADFQYSFNIMYFLTSINKPL
                     ILEQNMQNLFVFKNETKPVISNGIEANIKFIYKNDLKLFVGYTLLDAKAIYKTVNNFI
                     ALTPKNKLNFVLMYEKHNNFKLGFEGYYSDKQYLSNGTKTPNFIELGFLAEKTWKKLT
                     VFINFENFTDERQSNYKRVVNAPNTNPTFDEIWNHTEGRVINGGIKIKL"
     gene            complement(2551..2841)
                     /gene="secG"
                     /locus_tag="PLY81_05815"
     CDS             complement(2551..2841)
                     /gene="secG"
                     /locus_tag="PLY81_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNF29777.1"
                     /translation="MVLIFVILVVLASVALGFVILVQNPKGGGLSGNIGGIGNQFMGV
                     KQTTDMLEKGTWMFVSIIAVLAIVSSLLFKNVSSDSNLKKQIDKIDTRTPAK"
     gene            complement(2844..3944)
                     /locus_tag="PLY81_05820"
     CDS             complement(2844..3944)
                     /locus_tag="PLY81_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29778.1"
                     /translation="MHIALRKIFHQFSGKTNLEDTRIELLFEAAENEPYFAPAQFFLA
                     LKMKSENHSNLSAQLNKTALYFNNIHWLHYQLVPKPELIEFIEKEKTETTQPILQNVI
                     QEDIKNHNSQINEDTNSEKVEDAVVENNIPLEAEITEAIIEKPIEFEKEKTDFVPKEN
                     QSITALVLSNLDNEEVLNPIQTIHTKNDVPMLDTNETDLNEDVQNEPDKTNRTEVELE
                     NKLSTILKEQLEEYQQPVTKESELQFEQSPHHTVDYFESQGIKAEINLNSNDKLSTQL
                     KKFTDWLKHMKNLNLEQEKDLGTDPELENAIQGIAQNSNEAREIVTETMAEVLEKQGK
                     KDKAIQLYIKLSFLNPDKSSYFAAKIQHLKGI"
     gene            complement(4020..4535)
                     /gene="lptE"
                     /locus_tag="PLY81_05825"
     CDS             complement(4020..4535)
                     /gene="lptE"
                     /locus_tag="PLY81_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNF29779.1"
                     /translation="MHPSFSKNIRICFLYAFLFFLAACKIYSFKDVSIPPNIKTIKIG
                     FIENKARIVNPQLSPMLTDKIQQKITNQTRLTRTNSEDAHLQIEGYISDYSVTTTGVS
                     AQQEVTNRLTVSLQINLKNTLENSSKDFTITRSFDFSANLSLDQAQISLQEEIVRSLA
                     DDIFNRIFSNW"
     gene            complement(4560..>4877)
                     /locus_tag="PLY81_05830"
     CDS             complement(4560..>4877)
                     /locus_tag="PLY81_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF29780.1"
                     /translation="KESILTDFSNTPTTEVKQVNQQPQIVQPTFVQPIHSTNNQAVII
                     NDEIQQHEVVEESLNIMDKEKELIIKALKKHKSRRKDASADLGISERTLYRKLKEYDI
                     EDL"
BASE COUNT         1692 a          658 c          753 g         1774 t
ORIGIN      
        1 tgcagataat catactaaag atagtgaaga cttttgtagc ccattttgtg tttgtttttg
       61 ctgtagaaac ctagtacttc aacaagatga aataaaagat ttagaagtgc aaccgactat
      121 attttattta gcctcacaaa gcatatattc tactgctact attccaactg cgttcttaaa
      181 taatatttgg caacctccca aagtgtaatc tttttactgc cattatcaaa tattcaatat
      241 atacaattga atttgattat ttaaatatta ttaaagacta atttaaagaa ttttatgaaa
      301 aaaataatag cattactatt agtagtaatg gtttgtatta gtagcaatgt aaaagctcaa
      361 catacaattg ttactaaagt atttaatcat gagaacgacg aagttttaca aggcgtactc
      421 attaagattg caaaaacaaa tattagaaca gttaccaatg aaaatggggt tggagtatta
      481 aaaaatgttc ccaaaggtaa ttttaaaatt gaatttagct atactggctt tgagtctgct
      541 agtgtacagg ttacagtgcc acaagctaaa aatgatacat taattgtagc attacataat
      601 cacgaagagg aattagaaga agtatttgta caaagtacca gaacaagtag gactattaaa
      661 aattctccta ccagggttga aacaattagt ggagaagaac tagacgagaa aaacaatatg
      721 aggcctgcaa atgtgagtat gttaatacat gaaagtactg gaatgcagtt acaacaaacc
      781 agtgcaacta ctggaaatgc aagtattaga gtacaaggtt tagatggtag atatactcaa
      841 ttgctaaaag atggatatcc taattttggc aattttgcaa acgggttaag tgtattagaa
      901 ataccaccat tagatttaaa acaagtagaa attattaaag gacctgcatc aaccttatat
      961 ggtggtggag caattgctgg tgttgtaaat tttattagta aaacaccaaa agaaaaactg
     1021 gaaggtaatt ttattttaaa tcagtcgaat attgggcaaa gtaatattgc aggttattta
     1081 cagcagaaga aaggtaaatt tggttttact gtgttagcag cttacaatta ttcaaaggct
     1141 tatgatgtag ataaggatga tttttctgaa attccgaagt caaataatta tactatacat
     1201 ccaagattat ttttttatcc cgatagtaaa acaaccatta caataggtaa taatcttaca
     1261 caaagtaatt ctaaaggtgg cgatatgcaa gttatcaaag gcaatagttc tcttgggcat
     1321 gtttactttg aaaaaaatac taccacaaga aatacagtaa ctttagaggc agacagaaaa
     1381 attaattcag atagccgttt ttacttaaaa caaagtttta gtacatttaa tagaaatctt
     1441 caaaccaata atcatgagtt tgcaggctta agtactaata gttacacaga tttgagttat
     1501 acattcaata aaaataacca tactattatt tctggattaa atattattta cgacgatttt
     1561 aagcaacaca aagtaaccaa tgctttttta aattctaaaa gttttacaac aggcttttat
     1621 gctcaagaca cttgggatgt atctgaaata attaaaatag aaggtggttt aagggttgat
     1681 aatgttacat ataaaaatgt aaacttttct aaaaatcaaa cttttgtttt accaagagtt
     1741 tcagttttat taaattatag taaccattta agttctagaa ttggtgctgg tttagggtat
     1801 aaagtaccaa ctatttttac agaagaaaca gaagcaatgc actataataa tttacaagct
     1861 ttagataata gtgtaacttc tgaaagaagt gttggcggaa caattgatgt taattataaa
     1921 aacaaaatag gggcagattt tcagtatagt tttaatatta tgtacttttt aacttcaatt
     1981 aataagccat taattttaga acaaaatatg caaaatttgt ttgtatttaa aaatgaaaca
     2041 aagccagtaa ttagtaatgg aattgaagca aatattaagt ttatttataa aaacgattta
     2101 aaattatttg ttggttatac tttgttagat gcaaaagcaa tttataaaac agtaaacaat
     2161 tttattgctt taacacctaa aaataaacta aactttgtgt tgatgtatga aaagcataat
     2221 aattttaaat taggctttga aggctactat tcagataaac agtatttaag taatggtaca
     2281 aaaactccaa actttattga gttaggtttt cttgcagaaa agacttggaa gaaactaaca
     2341 gtgtttatca actttgaaaa ttttactgat gaacgtcaga gcaattataa aagagtggta
     2401 aatgcaccaa acaccaatcc aacgtttgat gaaatttgga atcatactga aggaagggta
     2461 attaatggag gcattaagat taagttataa taaaaataga ggctgcttat gggcagcctc
     2521 tatttttata aaagaaatta aataaataaa ttattttgcc ggagttctag tatctatttt
     2581 atctatttgc ttttttaggt tactatcgct actaacattt ttaaataata aagaggatac
     2641 aattgctaaa actgcaataa tgcttacaaa catccaagtt cctttttcta acatatcggt
     2701 tgtttgtttt acacccataa actgattacc aataccacca atattgccag acaaaccacc
     2761 accttttggg ttttgtacta aaataacaaa gcctaaagct acacttgcta atactactaa
     2821 gattacaaaa attaatacca taattatata ccttttaaat gttgaatttt ggctgcaaag
     2881 taagaggatt tatcaggatt taaaaaactt aattttatat ataactgaat ggctttatct
     2941 tttttaccct gtttctccaa aacttctgcc attgtttcgg ttacaatttc tcttgcttcg
     3001 ttagagtttt gagctattcc ttgtattgca ttttcaagtt ctggatcggt gcctaaatct
     3061 ttctcctgtt ctaaatttaa gtttttcatg tgttttaacc aatcagtaaa cttctttaat
     3121 tgtgtggaaa gtttatcgtt agaatttaaa ttaatttcag cttttatacc ttgcgattca
     3181 aaataatcta cagtatgatg cgggctttgc tcaaattgta attcactttc tttcgtaact
     3241 ggttgttgat actcctctaa ttgctctttt aaaatagttg ataatttatt ttctaattca
     3301 acctctgttc tatttgtttt gtctggctca ttttgtacat cttcattcaa atctgtttca
     3361 tttgtatcta acataggtac atcatttttt gtatgaatag tttgaattgg gtttagaact
     3421 tcttcgttat ctaaattact taatactaaa gcggtgatag attggttttc tttaggtaca
     3481 aaatctgttt tttctttttc aaattcaata ggtttctcaa taattgcttc agtaatttca
     3541 gcttctaaag gaatattgtt ttctacaact gcatcttcaa ctttttctga attagtatct
     3601 tcatttatct gactattatg atttttaata tcttcttgaa ttacattttg aagaattggt
     3661 tgcgtagttt cagttttttc tttttcaata aactctatca attcaggttt gggtactaat
     3721 tggtaatgta gccagtgaat attattgaaa tataaagctg ttttatttaa ttgagcagat
     3781 aggttggaat gattttcgct tttcattttc agagctaaga aaaactgagc aggagcaaag
     3841 tagggttcat tttcggctgc ttcaaatagc aactcaattc gggtatcttc taaattagtt
     3901 ttaccagaaa attgatgaaa tatttttctt agagctatat gcataatatt gaaaataaac
     3961 aaaacaaata tacacgagat atttattagt ttattaaaat aaaattggtt aagttgaaat
     4021 taccaattag agaaaattct attaaaaata tcatctgcta aacttcttac aatttcttct
     4081 tgcaaggaaa tttgggcttg gtctaatgat aagtttgcag aaaaatcgaa gctacgagta
     4141 attgtaaaat ctttactact attttctaaa gtatttttaa ggttaatttg aaggctcaca
     4201 gtaagtctat tagttacttc ctgctgtgca cttacaccag ttgttgttac agaataatca
     4261 gaaatatagc cttctatttg taaatgtgca tcctcgctat tggttcttgt aagcctggtc
     4321 tggttagtaa ttttttgttg tattttatct gttaacatgg ggcttaattg tggattcaca
     4381 attctggctt tgttttcaat aaacccaatt ttaatagttt ttatattagg gggaatgctt
     4441 acatctttaa aagaataaat tttacatgct gctaaaaaaa atagaaaagc atataaaaaa
     4501 cagatacgta tatttttact aaagctcgga tgcattttga ttatttctgg tataaaaaat
     4561 tataaatctt caatatcgta ttcctttaac tttcggtata aagttctttc gctaatgcct
     4621 aaatctgcac tggcatcttt tcttctgctt ttgtgttttt ttaatgcttt tataataagc
     4681 tctttttctt tatccattat gtttaaagat tcttctacca cttcgtgttg ttgtatttca
     4741 tcgttaataa ttacagcctg gttattagta gaatgaatag gttgaacaaa agttggttga
     4801 acaatttgtg gctgttggtt tacttgttta acttcggtgg ttggtgtatt agaaaaatcg
     4861 gttaatatac tttcttt
//
LOCUS       DAOQHP010000149         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10028_length_4868_cov_4.776854, whole genome shotgun sequence.
ACCESSION   DAOQHP010000149 DAOQHP010000000
VERSION     DAOQHP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10028_length_4868_cov_4.776854"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..742)
                     /locus_tag="PLY81_05835"
     CDS             complement(5..742)
                     /locus_tag="PLY81_05835"
                     /inference="COORDINATES: protein motif:HMM:NF027719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5012 domain-containing protein"
                     /protein_id="HNF29781.1"
                     /translation="MKTINISKLLLVLLVTIVSFTGCKKDLQFSKATFLPEIKLKGDE
                     VVLVKFGTDFTDDGVDATVNGKTVPYTTKVVGLYRGGTTVDKNTPDIYQVTYSATNED
                     GFSASITRTVVVANTGDLVNSIEGLYTSTVGRGGPATPQYTNMEYVLIWKISGNTYGI
                     TDALGGYYDIGRAYGPGYAAIGTVITANDISTNSFSFTQANIPMFGNVVDISNFVVDA
                     STHSISFTGTGNFANSIFGINLKQVNF"
     gene            complement(763..2154)
                     /locus_tag="PLY81_05840"
     CDS             complement(763..2154)
                     /locus_tag="PLY81_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNF29782.1"
                     /translation="MASCEKVLDVNTDPNNSSTATPKLVLPTAQSQLGATIGYAWNWY
                     GGMWAQYWTGGYGVSTSQVEYYNMVGTDVDGSWTRAYARSLEDMYYLQKSDQPVYAGM
                     AKIMSAYLYQMLTDLHGDIPFSQALKGEVVNGGITAPKYDKQEDVYAALIPLIDDGIT
                     GVTTTGAGVQSPTSEDLIYGGNISKWVKFANTLKLKILVRKGDYVGAKALIDAGTPFI
                     TSASDDAKFRYFETSQNTNPLYARFMARTDVGMYYVASKTSVDKLDGLADPRINKIYT
                     PGSSGDLGVFSGDINDNTPLYPSGGVNTKFSRPSPLTFGATVPVYFISAWESYFLQAE
                     VLIRSGQDASALFGSGVQESFNYYGLGSAAGYVSSLSFGGSQDNQLNILAIQKWIAMN
                     GLQMVEGWLETLRFDRPGNDIFKGGIFHSPINNSLGANKFPSSFVIPNQETTYNPNAP
                     KNVTVTSKRFWDN"
     gene            complement(2226..>4868)
                     /locus_tag="PLY81_05845"
     CDS             complement(2226..>4868)
                     /locus_tag="PLY81_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF29783.1"
                     /translation="NNALIVIDGVPVINSTNSANQNDNLSFVDFGNRGNDFDPDNIES
                     VSILKGPAATALYGSRATSGVVLITTKSGKGSDKSDKKFNVTINSNISFQRAYLQIKR
                     QDKFGQGYDNNPDPIENFSWGPAFDGIVRPWTPTIVLPNGSTSQLIRPYSAVKNQLQD
                     AFNIGTTFKNGIALEGASDKFSYYFSYNNTTNNGIIMNTFYKRHALTANTTARLAKNL
                     TSKVNVQYSKVYQRAMTGSGTANFAGTLQSLLQQPANIPIFELRDYNSPYQNFNGYYG
                     GYTANPFYLMSHTNNDMKADNLLASTELEYKPVDFITLTARVGDNLTISNMSQQQPKY
                     KYDRQKDGVNGGYTEELEKRNQLNIDLFGTFNKDVVKDVNINAMLGFNYLKSKYTDIF
                     GKTQNGIVIPGYYNLNNSVSKPYVETNYSNYGLIGLLGQVGIGYKNMLFGEFTARNDW
                     SSTLPAGKRGFSYFSGGLSFVPSELIKGDAKQWLNYLKIRANAGTQGKDAAPYMLSSV
                     FAAATFSDYYNDNLPYQFPVKGLDGSTVSGYTLGNTIGNAKLKPELTTAYEVGAEITA
                     LKEYIHVEYTFYHKTSKNLIVNANLPASSGFTRQVINVGKVRNIGHNLLVSVNAIKNL
                     KGVNWELRMQFAKNNSKVLKANIDGSDVILGGLTTASIIAKEGYPFGTFKANDFNRDS
                     LGRIIVGANGAPVTSTTQNIFGSFQPKYTMGWASTLSWKGLAFDVQFDMKKGGVFYSG
                     TREATDFNGTSLTSLLNNREPYIIPNSVTTTGAENTTPLTNLYTLVGSLPESQLLVDA
                     SYIKLREASVSYTFDKKFFKKAPISAISVALVGNNLKFWLPKENVFADPESNAWGQAG
                     NGQGMEYTSMPTSRSVAVDFKIKF"
BASE COUNT         1509 a          902 c          783 g         1674 t
ORIGIN      
        1 catactaaaa atttacttgt tttaaattaa ttccaaaaat agaattggca aaatttcctg
       61 ttcctgtaaa tgaaatagaa tgggtagaag catctacaac aaaattacta atatcaacta
      121 catttccaaa catagggata tttgcttgag taaatgagaa tgagttagta ctaatatcat
      181 ttgctgttat aacagtaccg atagcagcat aacccggtcc ataagctctt ccaatatcat
      241 agtatccacc tagtgcatct gtaatgccat aagtattacc tgatattttc caaattagaa
      301 catattccat gttggtgtat tgcggtgtag ccggaccgcc tctacctact gtggaagtgt
      361 aaagtccttc tatactgttt actaaatctc ccgtattagc aacaactact gttcttgtaa
      421 tagatgcaga gaagccatct tcatttgtag cggaataagt aacctgataa atatccgggg
      481 tgtttttatc tactgtagtt cctcccctgt atagtccaac tacttttgta gtataaggta
      541 cagttttacc attcacagtt gcgtcaactc catcatctgt aaagtcagtg ccaaatttaa
      601 ctaaaacgac ctcatctcct tttaatttaa tttctggtag aaacgtggct ttagaaaatt
      661 gtaaatcttt cttgcaacct gtaaacgaga caattgtaac aagtaaaact aataataatt
      721 tgcttatatt tattgttttc atactaaaca tttttattgt tattaattat cccaaaatct
      781 tttagaagta acggttacgt tctttggagc attaggatta taagttgttt cttgattagg
      841 aataacaaaa gaagaaggaa acttattcgc tcctaaagag ttattgattg ggctatggaa
      901 aattcctcct ttaaagatat cattacccgg tctgtcaaat cgaagtgttt ctaaccagcc
      961 ctctaccatt tgcaatccat tcattgcaat ccatttttga atagctagaa tatttaactg
     1021 attgtcttga gatccgccaa agctaagtga agatacataa cctgctgcag atcctaagcc
     1081 gtaataatta aaggattctt gtactccact accaaacaat gcagatgcat cttgaccact
     1141 acggattaac acttctgctt gtaagaagta actttcccaa gcgctaataa agtaaactgg
     1201 aacagttgca ccaaaagtta atggactagg tctgctgaat ttagtattaa caccgccaga
     1261 agggtataat ggagtattat cattaatatc tccggaaaac acacctaaat cgccagaact
     1321 acctggagta tagattttat taattctagg atcggctaag ccgtctaatt tatcaacact
     1381 agtttttgaa gctacataat acatcccaac gtctgttcta gccataaaac gagcataaag
     1441 tgggttagta ttttgagagg tttcaaaata tctaaattta gcatcatcac tagcagatgt
     1501 aataaaaggt gtgccggcat cgattaatgc tttagctcct acataatctc cttttcttac
     1561 caatatttta agtttaagag tgttagcaaa ttttacccat ttactaatat tacctccata
     1621 aattaaatcc tcagaagtcg gtgattgtac acctgccccc gtagtagtta cacctgtaat
     1681 accgtcatcg attaaaggta ttaatgctgc atacacatct tcttgtttgt cgtattttgg
     1741 agctgtaata cctccattaa ctacttctcc tttcaaggct tgagaaaatg gaatatcgcc
     1801 gtgtaaatct gtcaacattt gatataagta tgcagacatt attttagcca tacctgcata
     1861 aacaggttga tctgattttt gtaagtagta catgtcttct aaagaacgag cataagctct
     1921 agtccaacta ccatctacat ctgttcctac catgttatag tattcaactt gagaagtaga
     1981 gacaccatat ccgcctgtcc aatattgagc ccacatacct ccgtaccaat tccaagcata
     2041 tccaatagta gctcctaatt gagattgtgc ggtaggaagt actaatttag gtgttgccgt
     2101 acttgagtta ttcggatctg tgtttacatc taacactttc tcgcatgatg ccatgccgat
     2161 tgtaattgaa agtgcaagca gtaatccttt gatattatat attttcattt tcatataatt
     2221 acttgttaaa atttaatttt aaaatcaact gctacagagc gactagttgg cattgaagtg
     2281 tattccatac cttgtccatt accggcttgt ccccatgcat ttgactcagg atctgcaaat
     2341 acgttttctt taggcaacca gaatttcaag ttatttccaa ctaatgcaac agagattgct
     2401 gaaataggtg cttttttaaa gaattttttg tcaaaagtat agctaacact agcttctctc
     2461 aatttaatgt agctagcatc tactaacaat tgagattccg gtaaactacc tactaatgta
     2521 tataaattag ttaatggggt ggtattctcc gcacctgtag ttgttacact atttggaata
     2581 atatatggtt cgcggttatt caataatgat gttaatgatg taccattaaa atctgtagcc
     2641 tctcttgtgc ctgaatagaa cacaccgccc tttttcatat cgaattgtac atcaaaagcc
     2701 aatcctttcc aagaaagtgt cgatgcccaa cccatagtat atttaggttg gaatgaacca
     2761 aaaatatttt gtgtagttga agttacagga gcgccgtttg caccaacaat aattcttcct
     2821 aaagaatctc tattgaaatc attagctttg aatgtaccga aaggatatcc ttcttttgca
     2881 atgatagaag cagttgttaa accacctaaa attacatcac taccatctat attagctttc
     2941 aatactttac tgttattttt tgcaaactgc attctaagtt cccaattaac tccttttagg
     3001 tttttaattg catttacact tactaataag ttatgcccga tatttcttac tttaccaacg
     3061 ttgattactt ggcgagtaaa acctgaagaa gcaggtaggt ttgcattaac aatcaaattt
     3121 tttgatgttt tgtgatagaa tgtatattca acatgtatat attctttcag tgctgtaatt
     3181 tctgcaccta cttcataagc tgttgttaat tccggtttta attttgcatt accgatagta
     3241 ttacctaagg tataaccact tacggtagaa ccatctaaac cttttacagg gaattggtac
     3301 ggtaaattgt cattatagta gtcactaaat gttgcagcag caaatacaga gcttaacata
     3361 taaggcgcag cgtctttacc ttgtgttccg gcatttgcac gaattttcaa gtaattcaac
     3421 cattgttttg catctccttt tattaattcg ctgggtacaa atgaaagacc gccagagaaa
     3481 taggagaatc ctctttttcc agcaggaaga gtagaagacc agtcattacg tgcggtaaat
     3541 tctccaaaca acatattttt ataaccaata cctacttgtc ctaataaacc aatcaatccg
     3601 tagtttgaat aatttgtttc tacatacggt ttacttacag agttatttaa gttataatat
     3661 ccaggaataa caataccgtt ttgtgtttta ccaaaaatat cggtgtattt actttttaaa
     3721 tagttaaagc ctaacatcgc attgatatta acgtctttta ctacatcttt attgaatgta
     3781 ccaaataaat cgatgtttaa ttgatttctt ttttctaatt cttcagtata tccaccattt
     3841 actccatctt tttgtctgtc gtatttatat ttaggttgtt gttgtgacat gttagagatg
     3901 gtaaggttat cacctacacg agctgttaat gttatgaaat caaccggttt gtattctaat
     3961 tctgtagatg ccaacaagtt gtctgctttc atatcattat tagtatggct cattaaatag
     4021 aatggattag cagtgtatcc tccataatat ccattaaaat tttgatatgg gctattgtaa
     4081 tctctcaatt cgaaaattgg aatatttgcc ggttgttgta ataatgactg taaagtacca
     4141 gcaaaattag ctgttccaga acctgtcatt gctctttgat atactttaga gtattgtaca
     4201 tttacctttg aggttaaatt ctttgctaat cttgctgttg tatttgctgt taaagcatgt
     4261 cttttataaa acgtattcat aataataccg ttattcgttg tattattata agagaagtaa
     4321 taagagaatt tatctgaagc tccttctaag gctataccat ttttaaaggt tgtacctata
     4381 ttaaatgcat cttgcaattg attttttacg gcagaatatg ggcggattaa ttgagaagtt
     4441 gacccattag gcaatacaat agttggtgtc caaggacgaa ctataccatc aaatgcaggt
     4501 ccccaagaga agttttcaat aggatcgggg ttattatcat atccttgtcc aaatttatct
     4561 tgtcttttaa tttgcagata cgctctttgg aaagagatat tagaatttat agttacgtta
     4621 aacttcttat ccgatttatc cgatccttta ccactttttg tagtaatcaa cactacaccg
     4681 cttgtagcac gagaaccata caaagctgta gcagcaggtc cttttaagat agataccgat
     4741 tcgatattat ccgggtcaaa gtcattccca cggttaccaa agtcaacgaa gcttaagttg
     4801 tcattttgat tggcagagtt ggtactatta ataaccggca ccccgtctat aacaattaag
     4861 gcattatt
//
LOCUS       DAOQHP010000150         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10234_length_4803_cov_4.991575, whole genome shotgun sequence.
ACCESSION   DAOQHP010000150 DAOQHP010000000
VERSION     DAOQHP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_4803_cov_4.991575"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /gene="rho"
                     /locus_tag="PLY81_05850"
     CDS             complement(<1..1230)
                     /gene="rho"
                     /locus_tag="PLY81_05850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNF29784.1"
                     /translation="MYDILQLNDMLLPELLDIAEELKISNAKKLDKQDLIYKILDSQA
                     VKASETKEAEPKKQRIRKAVKTATANITEEAEIVNEPPRVVERKHIKPKKQKPDTNEN
                     VVPTDNVATTQQVADTVIENLAEKMLMATEDNTNTPEDNTKSTPPAKQHFHKKEPAFN
                     IEFDGVITSEGVLEMMPDGYGFLRSSDYNYLSSPDDVYVSPSQIKLFGLKTGDTVTGY
                     VRPPKEGEKYFALLKVDAINNKRPDEVRDRVPFDYLTPLFPFEKLNLFTTPQSYSTRV
                     MDLFTPIGKGQRGLIVAQPKVGKTMLLKEVANAIAANHPECYLMVVLIDERPEEVTDM
                     ERSVKAEVIASTFDEPAEKHVKVSSIALQKAKRLVECGHDVVILLDSITRLARAHNTV
                     APSSGKVLSGGVEANAMQ"
     gene            1607..3058
                     /gene="asnS"
                     /locus_tag="PLY81_05855"
     CDS             1607..3058
                     /gene="asnS"
                     /locus_tag="PLY81_05855"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNF29785.1"
                     /translation="MFNKRIKVKELLQIDAKGQEVTVMGWVRTFRNNQFIALNDGSTN
                     QNIQIVAELGLLNEDSLKRITTGASLKICGKVVPSLGKGQIVEVKAITIEVLGDCDAE
                     KYPLQPKKHSLEFLREIAHLRFRTNTFSAVFRVRHALAFAIHQFFNERGFVYLHTPII
                     TASDAEGAGEMFRVTTLPIDGSAKRNDEGTIDFTEDFFGKSTNLTVSGQLEAELGAMA
                     FSEVYTFGPTFRAENSNTTRHLAEFWMIEPEVAFNDIEDNMDLAENFIQYIIKYVMNN
                     NKEDLEFLDARLAEEEKQKPQNERQEFGLLEKLNFVTENKFERITYTEAIDILINSPA
                     YKKKKFKYDVSWGIDMQSEHERYLVEKHFKKPVIVTGYPAAIKAFYMRLNEGCEPGKE
                     TVAAMDILAPGIGEIVGGSQREERYDVLLAKMKQFDIPAQEMNWYLDTRRFGTVPHAG
                     FGLGFERMVQFVTGMTNIRDVIPFARTPKNCEF"
     gene            complement(3339..4295)
                     /locus_tag="PLY81_05860"
     CDS             complement(3339..4295)
                     /locus_tag="PLY81_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29786.1"
                     /translation="MKRIMIAAICFISLQAVKAQIEGITAFEQPSTSKITPKLKVSIN
                     TKQANSIDVSQDNKAFANFTTQQLVDGWERTKTSVYSSSTKDAFGTIAVSIIYLKVKR
                     LPDNSGMWVVRQAGLLNPLTKKTDYSTRDSTLFPNPFFKKTTKPIPGTKLEWHQEFTI
                     SNIEFETLFVDDEKVDPYRKTSEYAGLLEFKIFTKNGTQKKLIKNGGIIFNKTANQNA
                     ISYVPMEIGKKYPALGNPTFNFSISQTDFEKGYISIAGSLSDMDPTKAIESSTIGLMT
                     TLQELNNSTNKDLKDCVYQKNYIDIKKGDYTFRVHFIIKPVG"
     gene            complement(4335..>4803)
                     /locus_tag="PLY81_05865"
     CDS             complement(4335..>4803)
                     /locus_tag="PLY81_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF29787.1"
                     /translation="RAFEADIQYAPLFATNIWDGSAFLANLRVAEGLPDTRNKLITEG
                     KFVTNGILFEFQKAEVKPESYAVIKEIATVLKENPTIKIKITGHTSNDGDANANLTLS
                     KQRAAAVQSVLTNEFGIDASRMQTDGKGGSEPVDTTNTPTGKANNRRVEFIKL"
BASE COUNT         1512 a          734 c          837 g         1720 t
ORIGIN      
        1 ttgcattgca tttgcctcta caccaccact tagcacttta ccactacttg gtgcaactgt
       61 attatgtgca cgggctaaac gagtaatact gtctaaaaga attacaacat catgcccaca
      121 ctctaccaaa cgttttgctt tttgtaaagc aatgctggat acttttacat gtttttctgc
      181 tggttcatca aaagtagatg ctatcacttc agcctttaca cttctttcca tatcagtaac
      241 ctcttctggt ctttcatcaa tcaacactac cattaaataa cattcaggat ggttggctgc
      301 aatggcattg gctacctctt taagcaacat ggttttaccc acttttggtt gagcaactat
      361 taatccacgt tgtcctttac caattggtgt aaataaatcc attacacgtg tgctgtaact
      421 ttgtggtgta gtaaataaat ttaatttctc aaaaggaaat aaaggagtta aataatcaaa
      481 aggtacacgg tctcttactt cgtctggtct tttgttattg atggcatcta ccttcagtaa
      541 agcaaaatat ttttctcctt cttttggtgg ccttacatag cctgtaactg tatcgccagt
      601 ttttaagcca aaaagtttta tttgagaagg ggaaacatac acatcatctg gggatgaaag
      661 ataattataa tcgctacttc ttaaaaagcc ataaccatct ggcatcattt ctaaaacacc
      721 ttcgcttgta ataactccat caaattcaat attaaatgcc ggttcttttt tatgaaaatg
      781 ttgtttagca ggtggtgtac ttttagtatt gtcttctggt gtgtttgtat tatcttctgt
      841 agccattaac attttttcag ctaagttttc tataacagta tctgcaactt gttgtgtagt
      901 agcaacatta tctgtgggaa ctacattttc atttgtatcg ggcttttgtt ttttaggttt
      961 tatatgcttt ctttctacaa ctcttggagg ttcatttaca atttctgctt cttctgtaat
     1021 gttagctgtg gcagttttca ctgctttacg aattctttgt tttttaggtt ctgcctcttt
     1081 tgtttcgctg gcctttactg cctggctatc taaaatttta taaataagat cttgtttgtc
     1141 taattttttg gcattgctaa tttttagttc ttctgctata tctaataatt ctggtaatag
     1201 catgtcgttt aattgcaaaa tatcgtacat aatttgttat tgagtaataa agtttacagg
     1261 attatttttt gaaaaatgaa ttaatttcaa cggttagtat aaaataatta aaataataaa
     1321 aaaggtttgt gctgcttatt ttgtttggat aagaaagtaa actgatattt ttctggagat
     1381 gttttcttta gattctttca gctcgtttct gatgcaatgt taagacagag ttgtttttta
     1441 aaaaaatttt ttttttactc tacttctgtc aattatttat taaacacctc tcaagcttat
     1501 atttgccgtt ggttgaatac ttgttgccaa ttgaaaaaaa gtacaagagt gcgacgcaac
     1561 aactgttgaa tagtgaagta tattttgata cccaaaaaat aaaattatgt ttaacaaacg
     1621 aattaaagta aaagaattat tacaaataga cgccaaagga caagaagtta ctgttatggg
     1681 ttgggtgaga acctttagaa acaaccagtt tattgcatta aatgatggta gcacaaatca
     1741 aaatattcaa attgtggccg aacttggttt gctgaatgag gattcattaa aacgtataac
     1801 tactggagct tctttaaaaa tttgcggcaa agttgtacca agtttaggaa aggggcaaat
     1861 agttgaagtt aaagcaataa caatagaagt tttaggagat tgcgatgccg aaaaataccc
     1921 tttgcaaccc aaaaaacata gcttagaatt tttaagagaa attgcacatt tgcgttttag
     1981 aactaatact tttagtgcag tttttagagt gaggcatgct ctagcttttg ctatccatca
     2041 gttctttaat gaacgtgggt ttgtgtattt acatactcct attattacag caagcgatgc
     2101 tgaaggagca ggcgaaatgt ttagagtaac tacattacct atagatggta gtgcaaaaag
     2161 aaatgacgaa ggtactattg attttactga agactttttt ggcaaaagca ctaacctaac
     2221 tgttagtgga cagttagaag ctgaattggg ggcaatggct tttagtgagg tttatacttt
     2281 tgggccaacc tttagggctg aaaacagcaa tactacaagg catttagccg agttttggat
     2341 gattgaacca gaagttgcct ttaatgatat tgaagataat atggatttgg cagagaattt
     2401 tattcagtac attattaaat atgtaatgaa taataataaa gaagaccttg aatttttaga
     2461 tgccagatta gcagaagaag aaaaacaaaa gccacaaaat gaaaggcaag agtttggcct
     2521 tttagaaaaa ttaaattttg taactgaaaa taaatttgaa agaattacct ataccgaagc
     2581 tattgatatt ttaataaata gcccagctta taagaaaaag aaatttaagt atgatgttag
     2641 ttggggcatt gatatgcaaa gtgaacatga aagatattta gttgaaaaac attttaaaaa
     2701 gcctgtaata gtaactggct accctgcagc tattaaagca ttttacatga gattaaatga
     2761 aggttgcgag ccaggtaaag aaaccgttgc agcaatggat attttggcac ctggtatagg
     2821 tgaaattgta ggtggcagtc aaagagaaga aagatatgat gtgttactag caaaaatgaa
     2881 acaatttgat attcctgcac aagaaatgaa ctggtattta gataccagaa gatttggaac
     2941 tgtacctcat gcaggttttg gtttaggctt tgaacgtatg gtgcaatttg taacaggcat
     3001 gacaaatatt agagatgtaa taccttttgc aagaacacca aaaaattgtg agttctaata
     3061 actgaaattt acttaataat tatttgtatg caagtattat caaaagcaat atcttaaaaa
     3121 caattcgaac aataaagcag tgcaatagtt ttataaattg ctggatacag ataaaacatt
     3181 tgaagaacaa gtagcttttc agcaacagct aaaagcagcc ttacatttga ataataagct
     3241 gcaattaaaa gtacaatgaa caaagacaat tgaaatgatg aatataaaat aaaggctgca
     3301 cttgtgcagc ctttatttat ttgaatagaa aaaattaatt atccgacagg ttttataata
     3361 aaatgaactc tgaatgtgta atcccctttt ttaatatcta tataattttt ttgatataca
     3421 caatctttca aatctttatt agtgctgttg tttaattcct gtaaagtggt catcaaacct
     3481 atagttgatg attctatggc ttttgttggg tccatatctg ataaagaacc tgctatactg
     3541 atataccctt tttcaaaatc tgtctgagaa atgctaaagt taaaagtagg attacctagt
     3601 gcaggatact ttttccctat ttccattggt acataactta ttgcgttttg gttggcagtt
     3661 ttattaaaaa taataccgcc atttttgatt aattttttct gagtgccgtt ttttgtaaat
     3721 attttaaatt caagtaatcc tgcatattca gatgttttac gataggggtc aactttttca
     3781 tcatctacaa acaaagtttc aaattcaatg ttggaaatag taaattcttg atgccattct
     3841 aatttagttc caggaatagg ttttgtggtt tttttaaaga atggattagg aaacagcgta
     3901 ctgtctctag tactataatc cgtttttttt gttaatggat tgagtaagcc tgcttgccgt
     3961 acaacccaca ttcctgaatt atcgggtaat cttttgactt ttaaatatat tattgaaact
     4021 gcaatagtac caaatgcgtc ttttgttgat gatgagtaca cagatgtttt ggttctttcc
     4081 catccatcga ctaattgctg tgtagtaaaa tttgcaaacg ctttattgtc ctgagatacg
     4141 tcaatactat ttgcctgttt tgtattaata gaaactttta gcttaggagt aattttactt
     4201 gtagaaggtt gttcaaaggc agttattcct tcaatctgtg cttttacagc ctgcaatgaa
     4261 ataaagcata ttgcagctat cattattctt ttcatgtttt ttgcttttag tgaaatgatt
     4321 tattgatttt ttatttaaag ttttataaat tctactcgtc tgttattggc ttttcctgta
     4381 ggtgtatttg tggtatcaac aggctcgctg cctcctttgc catctgtttg catacgagaa
     4441 gcatctatgc caaattcatt ggttaaaaca ctttgcacag cagcagctct ttgtttagat
     4501 aaagtaagat tggcattggc atcgccatca ttgctggtgt gtcctgttat ttttatttta
     4561 atggttggat tttcttttaa tacagttgct atttctttaa ttacagcata gctttcaggt
     4621 tttacttctg ctttttgaaa ttcaaacaaa ataccattag taacaaattt tccttctgta
     4681 atcagtttgt ttcttgtatc aggcaagcct tctgcaacac gtaaattagc aagaaaagca
     4741 ctgccatccc atatattggt ggcaaataaa ggagcatact gtatatctgc ttcaaatgct
     4801 ctt
//
LOCUS       DAOQHP010000151         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10388_length_4755_cov_3.941277, whole genome shotgun sequence.
ACCESSION   DAOQHP010000151 DAOQHP010000000
VERSION     DAOQHP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10388_length_4755_cov_3.941277"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1390
                     /locus_tag="PLY81_05870"
     CDS             26..1390
                     /locus_tag="PLY81_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29788.1"
                     /translation="MLSIISFVVPLEFRRDSVNGYWIVFVSIISFISFVIWLIYLLQF
                     NVFKRFGNFTALDSLKQLLLYYTCILCFMTFPFVFSVVEVVRVNSTYNSKELINDINK
                     INLTIAQIEYDSIQHNWNRDTVWLVADLNKIDTQQRDSIPKITQHSKYAAIDSATFNS
                     RKKITDSVIDLGNNLYVFVECPVYSFLNIYNAKDIGEDKILKSASIFNLVIKNYKPAP
                     KDSLQSLLKTILNKYSINNYQSSINTEFDEQGNNSHLLSTNEIFKKYNLWSVERSIEN
                     IFRIKNRWAFEEMKDILRVVLYFTLILSLLVYIFRHSTVKTFFLSLLTAKLLTIISGL
                     VIALFDFKGYAVYEWYFFYVIFFLFLSIMVFTQSSRKVVTGIAINLFTLIFAFLPLMF
                     VFYYNERTRLNYIINDIDYQEKMKTINLYLSISEIVLVVLILLIIPTFIFKMYRKWYA
                     LPES"
     gene            1555..3402
                     /gene="yidC"
                     /locus_tag="PLY81_05875"
     CDS             1555..3402
                     /gene="yidC"
                     /locus_tag="PLY81_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNF29789.1"
                     /translation="MKTDKNTIIGFVLLGILFIGFFWYNNKMAQSAIDHENKVKDSLA
                     KVEASKITPEIKAKAIEDSIKRDSTNKILKEGNFTGSANGSEKIETIETDILKINFTN
                     KGGRIQTVSLKKYNSYDSTPVVLGTANDKLGYNINTGNNAVANTNDLYFQLKSNQKNT
                     DGSQTIIYELADSAGRNIQHIFTIAPNTYMIDWKVSLTAANQLLNDGNLNISWQTQTT
                     QHEKSAEYERVQMSNICFLENKEFDYIMNKTEHTFEKPTNWMSVVQQFFNTTLVAENN
                     FNSGNVKWEKHTTDTTKILANVQANFQIKIATAATNTIPLKMYFGPNDYNILKKQAPE
                     MDRIVNLGRDMYSFVRPINQYIIMPVFNFFSSFIGNYGWVILLLTLFIRLITAPLTYS
                     SYLSGAKMKVLRPELDALKKKIGENDKQAFAMEQMKLFREAGVNPLGGCIPALLQIPI
                     FFALFSFFNSNIALRGENFLWAKDLSSYDDVISWGTNIWLVGNHISLFNITAVITSFL
                     ISIYNMGNTPTQDNPALKYMPYIFPFVLFFVFNKMPAALTWYYTVSNLVTLILQFIIQ
                     KYIIDHDKILAKIEAKRSNPKKQSKWQERYTQMVENQKKIQEMKNKTKK"
     gene            3496..4143
                     /locus_tag="PLY81_05880"
     CDS             3496..4143
                     /locus_tag="PLY81_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29790.1"
                     /translation="MKSTLIILTTFLLSVNTLLAQQRQRVIAECTVEFAVNTDDVKGI
                     DNATLETFKSTSKTLYIKGYQSRVNFNSTSFSQSVIYNKRTGDAVILREIGGNKFLTK
                     LDSLAWKKSNSRFENAQLTLLNETKIILGYECKKAILMLADSVSLNLYYTPGFIPSVS
                     EFEFQFKNVPGFVLEYESQDKSGAKIKYFATKINLNPVSSSKFDIPTSGYRILNN"
     gene            complement(4215..>4755)
                     /locus_tag="PLY81_05885"
     CDS             complement(4215..>4755)
                     /locus_tag="PLY81_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNF29791.1"
                     /translation="LPGVAVGLAWTYVGGDILFIEVALSEGKGELKLTGNLGNVMKES
                     ATTALTYLQVNAKKLGIDADNFSKKNIHIHVPEGATPKDGPSAGITILTALTSAFTNK
                     KVKPFLAMTGEITLRGQVLPVGGIKEKVLAAKRAGLKEIIMCWQNEKDVLEIDSNFIK
                     GIKFNYVKNMQQVLDIAII"
BASE COUNT         1728 a          644 c          689 g         1694 t
ORIGIN      
        1 ctaaatatta aatattctat atcatttgct tagtataata tcatttgtag ttccactaga
       61 gtttagaaga gattcggtta atggttattg gatagtcttt gtatctatta ttagttttat
      121 ttcttttgtt atttggctaa tttatttact tcaatttaat gtttttaaac gttttggaaa
      181 cttcactgca ttagatagct taaaacaatt actactttat tatacctgta ttttatgttt
      241 catgactttc ccttttgttt tctctgttgt agaagttgta agggtaaatt ctacatacaa
      301 ttctaaagaa ttgattaatg atattaataa aattaattta actattgcac aaattgaata
      361 tgattccata caacataatt ggaacagaga cacagtttgg ttggttgcag atttgaataa
      421 gatagataca caacaaagag attctatacc aaaaataact caacattcta aatacgcagc
      481 tattgattct gcaactttta atagccgaaa aaaaataact gatagtgtga ttgatttagg
      541 caataattta tatgtatttg tagagtgccc ggtgtattct tttttaaata tttataatgc
      601 aaaagatatt ggtgaagata aaattttaaa atctgcttct atctttaatt tggtaataaa
      661 aaattataaa cctgcaccaa aagatagttt acaatcactt ttaaaaacta ttttaaataa
      721 gtattcaatt aacaactacc aaagtagtat aaacactgaa tttgatgagc aaggtaacaa
      781 tagccattta ctaagcacca atgaaatttt taaaaaatat aatttatggt cggtagaaag
      841 aagtatagaa aatattttta gaattaaaaa cagatgggct tttgaagaga tgaaagatat
      901 tttaagagtt gttttatatt ttactcttat tttaagttta ttggtttata tatttagaca
      961 tagtacagta aaaacatttt ttctatcatt acttactgca aaattattaa ccatcatttc
     1021 agggctagta atagcattgt ttgattttaa aggctatgca gtttatgaat ggtatttttt
     1081 ttatgttatc ttttttttat ttctttcaat aatggtgttt acacagtctt caagaaaagt
     1141 agtaaccggt attgctataa atttatttac cttaattttt gcatttttac ctttaatgtt
     1201 tgtattttat tacaatgaaa ggacaaggtt aaattatata attaacgata ttgattatca
     1261 ggaaaaaatg aaaacaatta atttatatct ttctatttct gaaattgtgt tggtagtgct
     1321 tattctttta attattccta catttatttt taaaatgtat cgtaaatggt atgctttgcc
     1381 cgaaagttaa ttagtactaa aatttatttg ttttaaatta aaacaattca ttcaatctgt
     1441 tattggcata aataaatgca aatcagtatg ttattagtaa taaaatagtt ccaaacacat
     1501 attgccacat tcactttcag ttccctacct ttgccaatct tttaaaaaca gacaatgaag
     1561 acagataaga atacgattat tggttttgta cttttaggaa ttttatttat tggttttttt
     1621 tggtacaata acaagatggc tcaaagtgcc attgatcatg aaaacaaggt aaaagattct
     1681 ttagccaagg tggaggcttc aaaaataact ccagaaataa aagcaaaagc tattgaagat
     1741 tcaattaaaa gagattctac taacaaaata ttaaaagaag gcaactttac tggctctgca
     1801 aatggttcag aaaaaatcga aactattgaa actgatatat taaaaattaa cttcaccaat
     1861 aaaggtggca gaattcaaac tgtaagttta aaaaaatata attcttacga tagtacacca
     1921 gttgttttag gaacagcaaa cgataaatta ggctataata ttaatacagg taataatgct
     1981 gtagctaaca ccaacgattt atattttcaa ttaaaatcta atcaaaaaaa taccgatggc
     2041 tctcaaacta ttatttatga gttagcagat tctgctggta gaaatataca acatattttt
     2101 accattgcac ctaatacgta tatgattgac tggaaagtta gtttaacagc agctaaccag
     2161 ttattaaatg atggcaacct aaatattagt tggcaaactc aaactacaca acacgaaaaa
     2221 agtgcagagt acgaaagagt gcaaatgagt aatatttgtt tcttagaaaa caaagagttt
     2281 gactacatca tgaacaaaac ggaacatact tttgaaaaac caactaactg gatgagtgta
     2341 gtgcagcaat tttttaacac aacgttagtt gcagaaaata acttcaactc tggtaatgta
     2401 aaatgggaaa aacatacaac agatactaca aaaattttag caaatgttca agctaatttt
     2461 caaattaaaa tagctactgc agctactaac actattcctt taaaaatgta ttttggccct
     2521 aatgattaca atattttaaa gaaacaagct cctgagatgg acagaattgt gaatttggga
     2581 agagatatgt attcatttgt tcgtcctatt aaccaataca tcatcatgcc tgtttttaat
     2641 ttcttttcta gctttatagg taattatggt tgggtaattt tattgctaac tttatttata
     2701 cgtttaataa ctgcaccgct tacttatagt agttacttaa gtggtgcaaa aatgaaagta
     2761 ttgagaccag aattagatgc tctaaaaaag aaaattggag aaaatgataa acaagcattt
     2821 gcaatggagc aaatgaagct gtttagagaa gcaggtgtaa atcctttagg aggttgtatt
     2881 ccagcattat tgcaaattcc aatattcttt gctttattta gtttctttaa ctcaaatatt
     2941 gcactaaggg gcgaaaactt tttatgggct aaagacttat cttcttatga tgatgtgatt
     3001 agttggggta ctaatatttg gttagtaggc aaccatatta gtttgtttaa tattactgct
     3061 gttattacca gttttttaat atctatatat aatatgggta atacaccaac tcaagataat
     3121 cctgctctaa aatacatgcc ttatattttt ccgtttgtat tgttctttgt attcaataaa
     3181 atgccagcag ctttaacttg gtattataca gtatctaacc tggttacgtt aattttacaa
     3241 tttattattc aaaaatatat tattgaccat gataaaattt tagcaaaaat tgaagctaaa
     3301 agaagtaatc ctaaaaagca gagtaaatgg caagaacgtt atactcaaat ggtagaaaac
     3361 caaaagaaaa ttcaggaaat gaagaataag actaaaaaat aaaattgatg aagctcctat
     3421 aaaacaggag cttttttgta aacattggca taatattgta tatagttatt taagcattca
     3481 taaaaagaaa ctaaaatgaa aagtactttg ataatactaa ctactttttt actttctgtt
     3541 aacacactat tagcacagca aaggcagcgt gttattgcag aatgtactgt agaatttgca
     3601 gtaaatacag atgatgtaaa aggtatagat aatgctacat tggaaacctt taaatcaacc
     3661 agtaaaactt tatatataaa aggctaccaa agtagggtga attttaatag tacttcattt
     3721 tctcaatctg ttatttacaa taaaagaact ggcgatgctg taattttaag agaaataggt
     3781 ggcaataaat ttttaactaa attagatagc ctagcttgga aaaaatctaa tagcagattt
     3841 gagaatgcac aacttacttt attaaatgaa actaaaatta tattaggata cgaatgcaaa
     3901 aaagcaatct taatgttagc cgatagtgta tcgttaaact tatattatac accaggtttt
     3961 ataccatcag taagtgaatt tgaatttcaa tttaaaaatg ttcctggctt tgtgttagaa
     4021 tatgaatcgc aagataaaag cggtgcaaaa attaaatatt ttgcaacaaa aattaactta
     4081 aatcctgtgt cttcttcaaa gtttgatata cctacaagtg gttatagaat attaaataat
     4141 taattggaac tatttaaata aaaaatgctt ctctattatt tttagaagca ttttttataa
     4201 ataaattgtt tattctaaat tatagctata tctaaaacct gttgcatatt ttttacataa
     4261 ttaaatttaa taccctttat aaagttgctg tcaatttcta aaacatcttt ttcattttgc
     4321 cagcacataa ttatttcttt taatccggca cgttttgcag ctaatacttt ttctttaatt
     4381 ccaccaacag gcagcacttg gccacgtaag gttatttcgc cagtcatggc taaaaagggt
     4441 tttacttttt tatttgtaaa ggcagatgtt aatgcagtaa gaatggtaat tcctgcacta
     4501 ggcccatctt ttggggttgc tccttctggt acatgaatat gaatattttt tttgctaaaa
     4561 ttatctgcat ctatacctaa ttttttggca ttaacttgta aataagttag tgcggttgtt
     4621 gcactttctt tcattacatt tcctaagttg ccggtaagct ttaattctcc tttaccttca
     4681 cttagtgcaa cttcaataaa taatatatca ccacctacat aagtccatgc aagccctaca
     4741 gcaacaccag gtaag
//
LOCUS       DAOQHP010000152         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10424_length_4745_cov_4.493177, whole genome shotgun sequence.
ACCESSION   DAOQHP010000152 DAOQHP010000000
VERSION     DAOQHP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10424_length_4745_cov_4.493177"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..691)
                     /locus_tag="PLY81_05890"
     CDS             complement(92..691)
                     /locus_tag="PLY81_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNF29792.1"
                     /translation="MIKTGNPIISIYTEMTPNPETMKFVANKLLYPGKSIDFQDESSA
                     SPSPLAKELFSFPFIKSVFIASNFITLTKTSETEDWDDIIPTIKQFLKDYLENGGVVV
                     NEEEAAKVKQESSNTVSADDNDIVKRVKELLENYVKPAVEMDGGAIQFKSYNDGVVNL
                     MMQGSCSGCPSSMITLKAGIEGMMKRMIPEVKEVVAEAE"
     gene            complement(818..1519)
                     /locus_tag="PLY81_05895"
     CDS             complement(818..1519)
                     /locus_tag="PLY81_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNF29793.1"
                     /translation="MKQYKHLFFDLDHTLWDLEANAKETLHELYVKYNLLQIGIVDFD
                     LFFNKYSIHNERLWNRYTQGFIKQEELRWKRIWLTFLEYKIANEPLSKQIATDFLQIL
                     PTKKILFPYTIEILQYLTKKNYLLHLITNGFEAVQNNKLKNSNIHSFFKEVITSQGSN
                     SLKPNKEIFEYALNKSKASVHESLMIGDNLEADIVGGINIGMDTVYVNYLNIKASNIQ
                     PTYTITHLQQLEEIL"
     gene            1626..2324
                     /locus_tag="PLY81_05900"
     CDS             1626..2324
                     /locus_tag="PLY81_05900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF29794.1"
                     /translation="MNIIITGASKGLGKAFAKIFAAQNHTLLLCARNQQQLTETATEI
                     KEEFPLATIHYTTKDLSKKNDAIEFGNWCLQFGKPSILINNAGQFIPGSIYNEEDGIL
                     EKMIEINLYSAYHLTRAILPSMIKNKEGHIFNICSIASLKAYTNGGSYSISKYALMGF
                     SKNLREELKPFQVKVTTVYPGAVYTDSWSNTGVEENRIMEPNDIAKMVYSASLLSPQA
                     CVEDIVLRPQLGDI"
     gene            complement(2329..2898)
                     /locus_tag="PLY81_05905"
     CDS             complement(2329..2898)
                     /locus_tag="PLY81_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29795.1"
                     /translation="MKNLAFILVLFISLSATSQVNKFDSTMKNGKVGYRVICKNKLNN
                     KNSLRVKLIGFEETKDEIDIDIKGKVVGAEVDDLNKDGYPDLVVYIITDDAKRKGTVF
                     AIASNENKGILPILFPSISENEKLKVGYDGNDQFQLINGVLSHKFAVADSTATKEQYA
                     VARQVLYNVIKIETAWKFTVLRSIEIPRP"
     gene            3049..3594
                     /locus_tag="PLY81_05910"
     CDS             3049..3594
                     /locus_tag="PLY81_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF29796.1"
                     /translation="MHLDDVTLLQQFKQPALKEKAFTALVKKYQEKIYWHIRRLVVTH
                     DDANDVLQNMFIKVWNGLENFREESKLYTWLYRIATNESLTFLDSQKKKAVVGFSEVE
                     EGLSNKVKAEVNFDSNKLEWKLQLAIQQLPEKQRAVFTLRYYDEMPYEEMSKILETSE
                     GALKASYHHAAKKIQDFILDN"
     gene            3627..4307
                     /locus_tag="PLY81_05915"
     CDS             3627..4307
                     /locus_tag="PLY81_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29797.1"
                     /translation="MSNLNNIQDELLQVAPNIINLADKNVYTVPNNYFNCLSDVILIK
                     IKSSSLSSKSAKSNHPYLAPDNYFDEFSNKIASIASAQNRHFSEIEHELNEVAPLLNS
                     INKNNIYTTPTNYFEQNKINPKQTNSLKSKWWLYAAAAVFFGITCIGIIKYINTSNKQ
                     VNLSTELAKANDEELTDFLDNQPKPIFYTHQTDEEEFGIFEGTETDELIYFLNNQPQK
                     GETLIKGI"
     gene            4322..4732
                     /locus_tag="PLY81_05920"
     CDS             4322..4732
                     /locus_tag="PLY81_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29798.1"
                     /translation="MKQILILFTFVALSFSSLAQSNKRGEKVQALKVAYMTQVLKLTP
                     QEAQSFWPVYNTYFEESKKARMNNTNDELKYQEEALNIKKKYKPEFKKILNDESRANL
                     VFKAERDFLVELQKEVKKRIQQRQKNKMKHNEFE"
BASE COUNT         1722 a          732 c          646 g         1645 t
ORIGIN      
        1 gtacctcttt aaaaaactag catccattta attggtttaa aaaaccaatt aaatggatgc
       61 tctatgaaaa ttgataggtg ttaaaaaagt attattctgc ttctgcaact acttctttta
      121 cttcaggaat catgcgtttc atcattcctt caatacctgc ttttaaagta atcatactgc
      181 tagggcaacc actacagctt ccttgcatca ttaagtttac tacaccatcg ttatagcttt
      241 taaactgaat tgcaccacca tccatttcaa ctgcaggttt tacataattt tctagcaact
      301 cttttactct ttttactata tcattatcat cggcacttac agtattgctt gactcttgtt
      361 ttacttttgc agcttcttct tcattaacaa caacaccacc attctctaaa taatctttta
      421 aaaattgttt aattgttgga ataatatcat cccaatcttc tgtttctgat gtttttgtta
      481 gagtaataaa attacttgca ataaacacgc ttttaataaa cggaaaacta aacaattctt
      541 tagctaaagg agatgggctt gcactacttt catcttgaaa gtctatactc ttaccagggt
      601 acaataactt gttggcaaca aatttcattg tttccgggtt gggtgtcatt tctgtataaa
      661 tactaataat tgggtttcct gtttttatca taacaaaaaa tttaaacgct acaaagttac
      721 agcattaatt tatagcaata atcattgttt tgtaagcatt tcgctttttt cggttatcgt
      781 aatagttttt ttaacagtca atttatacaa ttagtttcta taaaatttct tctagttgtt
      841 gtaaatgggt aatggtataa gttggttgta tattacttgc cttaatgttt aaataattta
      901 catacactgt atccattcca atatttatac ctcctactat atctgcttct aaattgtctc
      961 caatcattaa actttcatgt acacttgctt tagatttatt taaagcatac tcaaaaattt
     1021 ctttattagg ttttaagctg ttactacctt gtgatgtaat tacctcttta aaaaatgaat
     1081 gaatattact attttttagt ttattgtttt gaactgcttc aaaaccattg gttattaaat
     1141 gtagtaggta atttttttta gttagatatt gcagtatttc tatggtataa ggaaatagaa
     1201 tttttttagt aggtaaaatt tgtaaaaaat cggtagcaat ttgtttcgat aagggttcgt
     1261 tagcaatttt atattctaaa aaagttagcc aaattctttt ccatctcaac tcttcttgtt
     1321 taataaaacc ctgtgtatat ctattccaca acctttcatt atgaatactg tatttgttaa
     1381 aaaacaaatc aaaatctaca ataccaattt gcaataaatt atattttaca taaagttcgt
     1441 gtaaagtttc ttttgcattg gcttctaaat cccacaaggt atgatcaagg tcaaaaaaca
     1501 aatgtttgta ctgcttcata attgcaaata aatgttattg tatttatatt ttagactgta
     1561 tataaataaa cttttaaaca gtttttctta aaaaaatttg tttcttcgtg aagaaataat
     1621 ttatcatgaa tattattata acaggtgcct ctaaagggct tggaaaagca tttgcaaaaa
     1681 tatttgctgc tcaaaatcat actttattat tatgtgccag aaaccaacaa caacttacag
     1741 aaactgctac agaaattaag gaagagtttc ctttagcaac cattcattat actacaaaag
     1801 atttaagtaa aaaaaacgat gctattgaat ttggtaattg gtgtttgcaa tttggaaaac
     1861 cctctatact aataaataat gctggacaat ttatccctgg ttctatttat aatgaagaag
     1921 atggtatttt ggaaaaaatg atagaaatta atttatatag tgcctaccat ttaactagag
     1981 caattctacc ttctatgatt aaaaataaag aaggacacat tttcaatatt tgctccatag
     2041 cttccttaaa agcttataca aatggtggta gttatagcat tagtaaatat gcattaatgg
     2101 gctttagtaa aaatttaaga gaagagttaa agccttttca agtaaaagtt acaacagttt
     2161 accctggtgc agtatataca gatagttggt ctaatactgg tgtggaagaa aatagaatta
     2221 tggagcctaa cgacattgct aaaatggtat attctgcttc tttattatct cctcaagctt
     2281 gtgtggaaga tattgtttta aggcctcaat taggagatat ttaatttatt aaggtctggg
     2341 tatttctata cttcttaata ctgtaaattt ccaggcagtt tctattttaa ttacattgta
     2401 taaaacctgt cttgcaacag catattgctc tttagtagca gtagaatctg caactgcaaa
     2461 tttatgtgag agcactccat taattaactg aaactgatca ttgccatcgt atcctacttt
     2521 tagtttttca ttttccgaaa tgcttggaaa tagaatgggt aaaatacctt tgttttcgtt
     2581 agatgctatt gcaaaaacag ttccttttct tttggcatca tcggtaataa tgtaaaccac
     2641 taaatcagga taaccatctt tatttaaatc atcaacttcg gcacctacaa ctttcccttt
     2701 aatatcaata tctatttcgt ctttagtttc ttcaaaccct attaatttaa cccttaaact
     2761 gtttttattg ttcaatttat ttttacaaat tactctataa cccactttcc catttttcat
     2821 tgtactatca aacttattaa cctggcttgt tgcacttaag ctaataaata atactaaaat
     2881 aaaagctaaa ttcttcataa ttaaagtaaa tctttagtgt aatacggcaa atgtaactaa
     2941 tagctttaaa aaataacagg atactaattt taaaatttta taaaaacata tactaatttt
     3001 aaactaatgt aaaaataaaa agtcttacaa cgcacaaaca ccattttcat gcatttagac
     3061 gatgtaacct tattacaaca gtttaaacaa cctgctttaa aagaaaaagc atttaccgct
     3121 ttagtaaaaa aatatcagga aaaaatatat tggcatataa ggaggctagt tgttacacat
     3181 gatgatgcta atgatgtgtt acaaaatatg tttataaaag tatggaacgg gttagaaaat
     3241 tttagagaag aaagtaagct ttatacctgg ttgtatagaa tagccaccaa cgaaagttta
     3301 acatttttag acagtcaaaa aaagaaagca gtagttggct ttagcgaagt agaagaaggg
     3361 ttaagcaata aagtaaaagc tgaagtaaat tttgattcta ataaattaga atggaagcta
     3421 caacttgcca tacaacaatt gccagaaaaa caaagggctg tttttacgct acgttattac
     3481 gatgaaatgc cctacgaaga aatgagtaaa atactggaaa ccagcgaggg tgctttaaaa
     3541 gcaagctacc atcatgcagc aaaaaaaatt caagacttta tattagataa ttaaacttta
     3601 taccccaaaa atggtctaac taaacaatga gcaatttaaa taacatacaa gatgaattat
     3661 tacaagttgc acccaatatt attaatttgg ctgacaaaaa tgtgtacact gtacccaata
     3721 attattttaa ttgtttgtct gatgttattt taataaaaat aaagtcttct tctctttcat
     3781 caaaaagtgc caaatccaat catccatatt tagcccctga taattatttt gatgaatttt
     3841 cgaataaaat agcctccatt gcatctgccc aaaacagaca tttttctgaa attgagcatg
     3901 aattgaatga ggtagctcct ttattaaatt caattaataa aaataatatt tatacaaccc
     3961 ctactaacta ttttgaacaa aataaaataa acccaaaaca aaccaattct cttaaaagta
     4021 aatggtggtt gtatgcagct gcagcagtat tttttggtat tacttgtata ggaattatta
     4081 agtatattaa tactagtaac aagcaagtta acctaagtac cgagcttgca aaagccaatg
     4141 atgaagaact tacagatttt ttagacaatc aacctaaacc tattttttac acacatcaaa
     4201 ccgatgaaga ggagtttgga atttttgaag gaaccgaaac cgatgaatta atttattttt
     4261 taaacaacca accccaaaag ggcgaaactt taattaaagg catataaatc aataacttac
     4321 tatgaaacag atacttattt tattcacctt tgtagcactt tcatttagtt ctttggcaca
     4381 aagcaacaaa agaggagaaa aagttcaggc tttaaaagta gcttatatga cacaagtact
     4441 taaacttaca ccacaagaag ctcaaagctt ttggccagtt tataatactt attttgaaga
     4501 gtctaaaaaa gccagaatga acaatacaaa cgatgaatta aagtatcagg aagaagcatt
     4561 aaatattaaa aagaaatata aacctgaatt taaaaaaatt ttgaatgatg aaagccgtgc
     4621 caaccttgtt tttaaagctg agagagattt tttagttgaa ttacaaaaag aagtaaaaaa
     4681 acgaattcag caaagacaaa aaaacaaaat gaaacacaac gaatttgagt aaaagtttaa
     4741 tttat
//
LOCUS       DAOQHP010000153         4722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10508_length_4722_cov_3.383973, whole genome shotgun sequence.
ACCESSION   DAOQHP010000153 DAOQHP010000000
VERSION     DAOQHP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10508_length_4722_cov_3.383973"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLY81_05925"
     CDS             complement(<1..834)
                     /locus_tag="PLY81_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF29799.1"
                     /translation="MLTLIRWMVGLLFIFSGLIKANDPLGLSYKMQEFFEAWQLHALH
                     NYTLFFAYGMNVLEVVAGIAVIVGWQIKKTVWLLLTLIVFFTFLTAYVLFSGKIKACG
                     CFGDCVPLTPVQTFTKDIILLILILILLFKVNSFRSSLNNNLSFAIVFIALIGTILFQ
                     NYVLKYLPVLDCLPYAKGKNLVTGMQLPKGALPDSFAITFKYKKDGKLIEFDQNNFPA
                     DFDDTYEYIDRYDKLVRKGTALPAITDFSLTTVFGNDTTQALLNSKTKYILLFAKDFN
                     NI"
     gene            complement(914..1399)
                     /locus_tag="PLY81_05930"
     CDS             complement(914..1399)
                     /locus_tag="PLY81_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29800.1"
                     /translation="MKKLGLTFLFFSIFLLAYGQDKLDRNTIIGKWVAFGMLYNEVYY
                     NLDTDSFYISPEKYDLLLSQNEDTIGAKKYLRNLMLSILPFNFNFKKNGNCTFENKNI
                     FERTTYAFDELKKIITIFNATTGKFINLEAYMKDNDLYINMNKDDSNSIKGCIIFKKI
                     K"
     gene            complement(1406..2041)
                     /locus_tag="PLY81_05935"
     CDS             complement(1406..2041)
                     /locus_tag="PLY81_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF29801.1"
                     /translation="MGKTKKPKHIAVAGNIGAGKTTLTEMLSKHYRWIPQFEDVDHNP
                     YLMDFYEDMPRWSFNLQIYFLNSRLNQLLDIQRGTETIIQDRTIYEDAHIFAPNLHDM
                     GLMSKRDFENYFRFFQTLKTMVQPPDLLIYLKASVPTLVGQIEKRGREYEENIRLDYL
                     KKLNELYNKWIDNYKEGPLLVIDCDKNKFAEHDEHLGEVISKVDSMLFGLF"
     gene            complement(2140..3144)
                     /gene="trpS"
                     /locus_tag="PLY81_05940"
     CDS             complement(2140..3144)
                     /gene="trpS"
                     /locus_tag="PLY81_05940"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNF29802.1"
                     /translation="MNKEIVLSGIRPTGFLHLGNYFGAMRNYIKMQEDYNCYFFVANW
                     HSLTTHPDTKELKNAVRRVIAENIACGLNTDKAVLYVQSDVPEIAELYLYLNMLAYKG
                     ELEKTTTFKDKARSQPDNVNAGLLTYPVLQAADILIHRAVKVPVGKDQEQNLEMARNF
                     AQRFNHRYGDIFPMPFAFNYGNELVKILSLDGNGKMSKSENQLATLYLADDDDTIRKK
                     ILKAKTDSGPTETNAIKPDYIQNLFTLMSFASSEDTVQKFEEDFNKSSSGNCIIRYGD
                     MKKQLAEDMINFIKPIREKAADLQQNTDLLNKIIKQGADKARDSASQTLQQVRKAVLG
                     "
     gene            3334..>4722
                     /locus_tag="PLY81_05945"
     CDS             3334..>4722
                     /locus_tag="PLY81_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF29803.1"
                     /translation="MRIEEIKVLKGPNFWSIRRNKLIQMKLNLEELEQKPTNKIDGFS
                     ERLEAMFPSMYSHRCSEAKPGGFFSRVKEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEEGKYYVVFSYMEEDAGTYAAKAAVKIAQALTDGTEYDLANDIQRLREIREDTRL
                     GPSTGCIVDEAAKRGIPHLRLNKHSLVQLGYGVHQKRIRATIASTTSNIAVDIACDKE
                     ETKLLLEAAEIPVPKGMVIRTEVGLKEAVDKYGYPLVIKPIDGNHGKGNTTNITTWEQ
                     ALKALENAKQYGRSVIVERFITGFDFRILVINYKFICAALRTPAAVIGDGTHNIQWLI
                     DETNKDPRRGYGHEKVLTQITVDHSTMKMLEEKGYTLETIPVKDELVLLKTTANLSTG
                     GTSTDVTDEVHPDNIVMCERIAKIVGLDICGIDIMAKDLHTPVTENGGAILEVNAAPG
                     FRMHIEPAIGLPR"
BASE COUNT         1678 a          727 c          701 g         1616 t
ORIGIN      
        1 tatattatta aaatctttag caaataataa aatatatttt gttttgctgt ttaataaagc
       61 ctgtgtagtg tcgttgccaa aaactgtagt taaagaaaaa tctgttatag caggcaatgc
      121 agttcctttt cttactaatt tatcgtatct atcaatatat tcataagtat catcaaaatc
      181 tgcaggaaaa ttattttgat caaactctat taatttgcca tcttttttat atttaaaagt
      241 tatagcaaaa ctatctggca aagccccctt aggtagttgc atacctgtaa ctaaattttt
      301 tcctttagca taaggcaaac aatctaaaac aggtaaatat ttcaacacat aattttgaaa
      361 taatattgtg ccaattaaag caatgaaaac aatagcaaat gataggttat tatttaagct
      421 actgcgaaaa ctattaactt taaaaagcaa tattaaaata agaattaaca aaataatatc
      481 cttagtaaaa gtttgaactg gtgtaagtgg tacacaatct ccaaagcaac cacatgcttt
      541 aattttacca gaaaacaata cataagctgt aagaaaagta aaaaatacaa ttagcgttaa
      601 aagcaaccac accgtttttt taatttgcca tccaacaatt acagcaatgc ctgccactac
      661 ttctaacaca ttcataccat aagcaaaaaa taatgtataa ttatgtaatg catgaagttg
      721 ccaagcttca aaaaattctt gcattttgta gcttaaaccc aatgggtcat tggcttttat
      781 taaaccagaa aaaataaaaa gtaaacctac catccagcga ataagtgtaa gcatagcaaa
      841 cgttttaaat tacttaaaaa atgaaaatag cttaaaatgg gtaactataa taccataact
      901 acaattatta aaattatttt attttcttaa aaataataca cccttttatt gaattagagt
      961 catccttatt catatttata tacaaatcat tatctttcat atatgcttca agattaatga
     1021 actttcctgt tgtagcatta aaaatggtaa ttattttttt taattcatca aaggcatagg
     1081 tggtacgttc aaaaatattt ttattttcaa aggtgcaatt tccattcttt ttaaagttaa
     1141 agttaaaggg caatatggaa agcattagat ttcttaaata cttttttgct ccaattgtat
     1201 cttcattctg gctcaataat aaatcgtatt tttctggtga aatataaaat gagtcagtat
     1261 ctaaattata atatacctca ttatatagca ttccaaatgc aacccattta ccaataatag
     1321 tatttctatc taacttatcc tgaccataag caagaagaaa aatagagaaa aataaaaaag
     1381 ttaaaccaag tttcttcatc gttttttaaa ataaaccaaa caacatacta tctactttac
     1441 taattacttc tcctaaatgt tcatcatgtt cggcaaattt atttttgtcg caatctataa
     1501 ctagtaaagg accttcttta tagttatcta tccatttgtt atacagttca tttaattttt
     1561 ttaaataatc caatcttata ttttcttcat attctctacc acgtttttca atttgtccta
     1621 caagtgttgg tacagatgcc tttaaataaa ttaataaatc tggtggttga accatggttt
     1681 ttaacgtttg aaagaatctg aaataatttt caaaatctct tttactcatt aaacccatgt
     1741 cgtgtaagtt aggagcaaaa atgtgtgcat cttcataaat cgtcctatct tgaataatgg
     1801 tttctgtacc tctttgtata tctaataatt gatttaagcg actatttaaa aaatatattt
     1861 gaagattaaa actccagcgt ggcatatctt cataaaaatc cattaaatac ggattatgat
     1921 ctacatcttc aaattgtggt atccaacgat aatgtttact taacatttct gtaagtgttg
     1981 ttttacctgc accaatatta cctgcaacag caatatgttt tggttttttt gttttaccca
     2041 taatatttta tattctaaaa aaaattattc catttaaagt cgtgaaagta agatttatcc
     2101 tttataattt cacaaataaa tagcatcaaa aaattatatt taacccaaaa cggcttttct
     2161 aacttgttgt aatgtttgcg atgcactatc tcttgcttta tctgctcctt gtttaattat
     2221 tttatttaat aaatctgtat tctgttgtaa gtctgcagct ttttctctaa ttggttttat
     2281 aaagtttatc atatcttctg ctaattgttt tttcatatcg ccgtatctta ttatacaatt
     2341 accagagctg cttttattaa aatcttcttc aaacttttgt acagtatctt ctgaactggc
     2401 aaaagacatt aaggtaaata ggttttgtat ataatctggt tttattgcat tggtttctgt
     2461 tggtccacta tcggttttag ctttaagtat tttttttcta atggtatcat catcatctgc
     2521 taaatataaa gtagcaagtt ggttttcact tttactcatt ttaccatttc catcaaggct
     2581 taaaattttt acaagctcat taccataatt aaaagcaaat ggcataggaa aaatatctcc
     2641 ataacgatga ttaaaacgct gagcaaaatt tcttgccatt tctaaatttt gctcctgatc
     2701 cttaccaaca ggtactttaa cagctctgtg aattaaaata tctgcagcct gtaatacagg
     2761 gtatgtaagc aatcctgcat taacattatc tggttgagaa cgagctttat ctttaaaggt
     2821 tgtagttttt tctaattcac ctttataagc taacatattc aaataaaggt atagctcggc
     2881 aatttcgggt acatcgcttt gtacatataa aactgcttta tctgtattaa gtccgcaagc
     2941 tatattttct gcaataactc ttcttactgc atttttaagc tcctttgtgt cgggatgtgt
     3001 ggttaatgaa tgccagttag caacaaagaa atagcaatta taatcttctt gcattttaat
     3061 ataattacgc atggctccaa aataattacc taaatgtaaa aatccggttg gtctaattcc
     3121 gcttaaaaca atttctttat tcataatggc aaaaatagtt gtttaagaat aagatgttaa
     3181 attgaaatgt attttaaaac gaataaatta aaagataaaa acatttaaaa atatagtttc
     3241 tattttcttt gatgtaaaat caattacgct tatcttacat tcctataaaa tatattttat
     3301 ttatcatcaa acatttcaac caataattag tgtatgagaa ttgaagaaat taaagtttta
     3361 aaaggaccaa atttttggag tattcgtcgt aataagctga tacagatgaa gcttaatctg
     3421 gaagaattag aacaaaaacc aaccaataaa atagatggtt ttagcgaaag attagaagct
     3481 atgtttccta gtatgtatag tcatagatgc agcgaagcta agccaggtgg tttttttagc
     3541 agagtaaaag aaggtacttg gatggggcat gtaattgagc atattgcatt agagctacaa
     3601 accctagcag gcatggatac aggctttggc agaacacgtg gcaccggcga agaaggaaaa
     3661 tattatgttg tatttagtta tatggaagaa gatgcaggca cttatgctgc aaaggctgca
     3721 gttaaaattg cacaagcttt aaccgatggt acagaatatg atttagctaa cgatattcaa
     3781 cgattaagag aaattagaga agataccaga cttggaccat ccacaggttg tattgtagat
     3841 gaagcagcaa aacgtggtat tccgcattta cgtttaaaca agcacagttt ggtacaattg
     3901 ggttatggtg ttcatcaaaa aagaataaga gcaactattg caagtacaac ctctaacata
     3961 gctgtagata ttgcctgcga taaagaagaa accaagcttt tattagaagc tgcagaaatt
     4021 ccggttccta aaggaatggt tatcagaaca gaagtgggat taaaagaagc cgtagataaa
     4081 tatggttatc cgttagtgat aaaaccaatt gatggaaacc atggcaaagg aaacaccaca
     4141 aatataacca cctgggaaca agcactaaaa gctttagaaa atgcaaaaca atatggtaga
     4201 agcgttattg tagaacgatt tattaccggt ttcgatttta gaattttagt tattaattat
     4261 aaatttattt gtgcagcatt aagaacacct gctgcagtta ttggagatgg aactcataat
     4321 attcaatggt taatagacga aaccaataaa gacccaagaa gaggttatgg tcacgaaaaa
     4381 gtattaacac aaattaccgt agatcatagc accatgaaaa tgctggagga aaaaggatat
     4441 accttagaaa ctattcctgt taaagatgaa ttagttttac ttaaaacaac tgctaacctt
     4501 agcacaggtg gtacaagtac agatgttaca gacgaggtgc acccagataa tattgtaatg
     4561 tgtgaacgta ttgcaaaaat tgttggtttg gatatttgtg gtattgatat tatggcaaaa
     4621 gacttacata cacctgttac agaaaatgga ggagccattt tagaagttaa tgctgctccg
     4681 ggttttagaa tgcatattga acctgccatt ggtttacctc gt
//
LOCUS       DAOQHP010000154         4682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10637_length_4682_cov_3.463583, whole genome shotgun sequence.
ACCESSION   DAOQHP010000154 DAOQHP010000000
VERSION     DAOQHP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10637_length_4682_cov_3.463583"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PLY81_05950"
     CDS             complement(<1..1460)
                     /locus_tag="PLY81_05950"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNF29804.1"
                     /translation="MNNLQDILYKVHIKQVIGTTQLNVKNVQIDSRCVSNGSVFIAIK
                     GDKTDGHHFIEQVIQNGAIAVICQTIPERIDPSITYVLVENTHEALAVLATNFYNNPS
                     KKINLIGVTGTNGKTTIATLLFKLFTQLGYKCGLISTVQNQIENKISPSTHTTPDAIK
                     LNELLKQMVEENCTHVFMEVSSHAIHQHRITGLEFKGGIFSNITHDHLDYHKTFDEYI
                     KVKKKFFDDLPSNAFAISNADDKRGAVMLQNTKASKHFYSLKSIEEFKGKILDNALTG
                     LQMLVNDKEVHFRLIGEFNAYNLLAVYATAICLNENKEKVLTALSMLTGAEGRFDYII
                     GNQLIIGIVDYAHTPDALENVLTTINKLRKGHEQIITVVGCGGDRDKTKRPLMAQTAC
                     DLSDKVILTSDNPRTESPEQILKDMEVGLNTAAKRKYITITDRREAIATAVNMAKGED
                     IILVAGKGHEKYQEINQIKYPFDDKEILNNLLNKQQ"
     gene            complement(1488..3605)
                     /locus_tag="PLY81_05955"
     CDS             complement(1488..3605)
                     /locus_tag="PLY81_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNF29805.1"
                     /translation="MEVKKDILWRVYLAFIVLVLVCFVIIGKAFYIQQIQGKHWRSMS
                     DSLHQRIEEIDAERGTIFSENGEMLSTSIPQFDIFIDFAAQGLREKNGKRFRENLDSL
                     SFCLSNLFKDKSPAEYKAILSKGFKEKKRYYPLMKNVSYRDYQQLKTFPLVRLGKNKS
                     GFITDDKSIRLNPYKMLAFRTIGLARDSFKVGLEMSYDSLLKGKTGRRLVRSIAGGVT
                     VPVEEYEINAEAGKDIVTTLDVFIQGVTENALMNMMVKNEAANGCAIVMETKTGKIKA
                     IANLGRNATDGSYFEDYNYAINLSEPGSTFKLATLIALLEDKKVNLNSTVNLQGGVWN
                     INGRTVYDSEKHGRNDVTVKQAFELSSNVGMAKLAWSGYGSSPSKFINHLQNLNLDKP
                     TGIDITGERKPIIFKPGSKFWSSTTLPWMSFGYNVSITPLQTLTLYNAVANNGVMLKP
                     YLLNSVQQEGKLLKEFNPTITKEKICSNETLKQLKECLEGVCTEGTAQSLFKNSPYKV
                     AGKTGTALVANGNKGYDDKIYQSSFAGYFPAEDPQYTIIVVIKNKPHALNYYGGTVAG
                     PVFKEISDRLYTTRVKHAPANSVGLQKKDSSTYIFSGNKMDIEQITQKLKLGFQDNSG
                     ITNEWVNITVKNENVAIRNKDINTNRMPALKGLGLKDALTICENKGLKINVKGKGKVI
                     SQSVVEGSIVANGQVINIQLGTL"
     gene            complement(3635..4012)
                     /locus_tag="PLY81_05960"
     CDS             complement(3635..4012)
                     /locus_tag="PLY81_05960"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNF29806.1"
                     /translation="MAENENIKNTNQPNEKKGLKKGVGVLFNSQWITNNLYFFLFLGL
                     LAIIYIANGHWADKTIRNIDKSKKELRDLQFEYKTIKAELMHKSEEAQVLKAAEPLGL
                     KISKEVPVRLPNDNEPTELRNKK"
     gene            complement(4019..>4682)
                     /gene="rsmH"
                     /locus_tag="PLY81_05965"
     CDS             complement(4019..>4682)
                     /gene="rsmH"
                     /locus_tag="PLY81_05965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNF29807.1"
                     /translation="LRLYNVQQVDGVLADLGVSSHQFDVGERGFSTRFDGPLDMRMNT
                     NENKTAAKVIQSFTEQQLHKIFEQFGEVTNAKTLAKHIVQNRTKQPLQTIEQFKMLIS
                     AVVKGNPNKYLAQVFQALRIEVNEEIDALKEMLLQLNNLVKPGGRAAIITFHSLEDRV
                     VKNFFRNGSFQNELSDNPFAIGEKKKTWKLINHKPIVANEDELKNNKRSRSAKLRVAE
                     KI"
BASE COUNT         1358 a          856 c          604 g         1864 t
ORIGIN      
        1 tattgttgtt tattcaataa attgtttaat atttctttat catcaaaagg gtattttatt
       61 tggtttatct cctgatattt ttcatgccct ttgcctgcaa ccaaaataat atcttcgcct
      121 tttgccatat ttacagcagt agcaatggct tctcttctat cggttattgt tatatatttt
      181 cttttggcag cagtgtttaa tcctacttcc atatctttta aaatttgttc aggagattct
      241 gttcttgggt tatcgctggt aagaattact ttatcgctta aatcgcaggc agtttgtgcc
      301 attaaaggcc tcttagtttt atctctatct ccaccacagc ctaccacagt aataatttgc
      361 tcatgccctt tccttaattt atttattgta gttaatacat tttctagagc atctggtgta
      421 tgagcataat ccacaattcc aataattaat tggttgccta taatataatc aaatctaccc
      481 tcggcacccg ttaacatgct taaagcagtt aatacttttt ctttgttttc atttaaacaa
      541 attgctgttg catacactgc taataaatta taagcattaa attctcctat taatctaaaa
      601 tgaacttcct tgtcatttac cagcatttgc aaaccagtaa gtgcattatc taaaattttt
      661 cctttaaact cttctattga ctttaagcta taaaaatgtt tagaagcttt ggtgttttga
      721 agcataacag caccacgttt atcatctgca ttacttattg caaaagcatt tgacggtaaa
      781 tcatcaaaga attttttctt tactttaata tattcatcaa aggttttgtg ataatctaaa
      841 tgatcatggg ttatgttgct aaaaatgccc cctttaaatt ctaggcctgt aattctatgt
      901 tgatgtattg catggctgct tacttccata aacacatgag tacaattttc ctctaccatt
      961 tgtttcagca actcatttaa ttttattgca tctggtgttg tatgagtgga aggagaaatt
     1021 ttattttcta tttgattttg cacagtactt attaacccac atttgtatcc taactgtgta
     1081 aataatttga ataataatgt tgcaatagtt gtttttccat tggtgcctgt aacaccaatt
     1141 aaattaattt ttttagaagg gttgttataa aaatttgtag ctaatacagc taatgcttcg
     1201 tgtgtatttt ctaccaaaac ataagtgatg cttgggtcaa ttctttctgg aattgtttga
     1261 caaataactg ctatagcacc attttgaatt acttgttcaa taaaatgatg tccatctgtt
     1321 ttatctcctt taattgcaat aaatacgctg ccattagaaa cacaacgaga atctatttgt
     1381 acatttttta cattgagttg ggtggtacca attacttgtt taatgtgtac tttgtataat
     1441 atgtcttgta agttattcaa gtgtttattc ttgttgttaa aataatttta tagtgttcct
     1501 aattgtatat taattacctg cccatttgct acaatacttc cttcaacaac agactggcta
     1561 attacttttc cttttccttt tacattaatt tttagtcctt tgttttcaca tattgttagt
     1621 gcatctttca accccaaacc ctttaatgct ggcattctgt tagtatttat atctttattt
     1681 cttattgcaa cattttcatt tttaactgta atatttaccc attcgttagt aatgccactg
     1741 ttatcttgaa aaccaagttt tagtttttga gtaatttgtt ctatatccat tttattacct
     1801 gaaaaaatat aggtgctact atcttttttt tgtaaaccaa ctgaattagc aggtgcatgt
     1861 tttactcttg tggtataaag tctatctgat atttctttaa atacaggacc agcaactgtg
     1921 ccaccataat aatttaaagc atgtggcttg tttttaataa caacaatgat ggtatattgt
     1981 ggatcctctg cagggaaata acctgcaaaa gagctttgat aaattttgtc atcataacct
     2041 ttattaccat ttgctactaa agcagtacct gttttacctg ctactttata tggagaattt
     2101 ttaaacaaac tttgtgcagt accttctgta caaacacctt ctaaacattc ttttaattgt
     2161 tttaatgttt cgttactaca aattttttct tttgtaattg ttggattaaa ctcttttaac
     2221 aattttcctt cttgctgtac tgaattgagc aaataaggtt taagcattac tccattatta
     2281 gccacagcat tatataaggt taatgtttga agtggtgtga tggaaacatt gtacccaaaa
     2341 ctcatccaag gtaaagtagt agaactccaa aacttgctgc caggcttaaa aataattggc
     2401 tttctttcgc ctgtaatatc aatacctgta ggcttatcta aatttaaatt ttgtaaatga
     2461 ttaataaatt ttgatggtga agaaccataa ccactccatg ctaattttgc catacctaca
     2521 ttgctgctta actcaaatgc ctgtttaaca gtaacatcat ttcttccatg tttttcgcta
     2581 tcgtaaactg ttcttccatt gatattccaa acaccacctt gcaagttaac tgtagagttt
     2641 aggtttactt ttttatcttc aagtaaagca attaatgtag caagtttaaa agtagatcca
     2701 ggctcagaca gattaatggc ataattataa tcttcaaaat aagaaccatc tgttgcattt
     2761 cttcctaaat tagcaattgc tttaatttta cctgtttttg tttccattac aatagcacag
     2821 ccattagctg cttcattctt aaccatcata ttcattaaag cattttctgt tacaccttgt
     2881 ataaaaacat ctaacgtggt aactatatct tttccagctt cggcatttat ttcatattct
     2941 tctacaggta ctgttacacc accagcaatt gaacgaacta accttcttcc tgttttgcct
     3001 tttagtaaac tatcgtaaga catttccaat cctactttaa aagaatctct ggctaaacca
     3061 attgttctaa atgctaacat tttataagga ttaagcctaa tgcttttatc atctgtaata
     3121 aatccacttt tattttttcc taatctaact aaaggaaaag tttttaattg ttgatagtct
     3181 ctatacgaaa catttttcat taatggataa tatcttttct tctctttaaa tcctttactt
     3241 aaaattgctt tgtattctgc tggcgattta tctttaaata aattgcttaa acaaaagctc
     3301 aatgaatcta aattttcacg aaatcttttt ccattttttt ctcttaaacc ctgtgctgca
     3361 aaatcaatga aaatatcaaa ttgaggaatg ctggtactta acatttcacc attttcgcta
     3421 aatatggttc ctctttctgc atctatttct tcaattcgct gatgcaaact atcactcata
     3481 cttctccaat gcttaccctg aatttgttgt atataaaatg ctttcccaat tattacaaag
     3541 catactaata ctagcactat aaatgctaaa taaaccctcc atagtatgtc tttttttact
     3601 tccatttttt ataaacaatc aataatttca atgcctactt tttatttctc aattcagttg
     3661 gttcattatc atttggtaat cttacaggta cttctttact aatttttaag cctaaaggtt
     3721 ctgcagcttt tagcacttga gcttcttcgc ttttatgcat caattctgct tttatggttt
     3781 tatattcaaa ttgtaaatcc cttaattctt ttttagattt atcaatattt ctgatggttt
     3841 tatccgccca atgtccattt gctatataaa taattgccaa taaaccaagg aataaaaaaa
     3901 agtataagtt gttcgttatc cattgtgagt taaacaatac tccaactccc tttttaagcc
     3961 cttttttttc gtttggctga ttggtgtttt ttatattttc attttctgcc attagatgtt
     4021 atattttttc agctactcta agttttgcac ttctgctacg tttattattt ttcagttcat
     4081 cttcgtttgc tacaattggt ttgtggttaa ttaacttcca agtttttttc ttttcaccaa
     4141 ttgcaaaagg attatctgac agctcatttt gaaatgaacc atttctgaaa aaatttttca
     4201 ctactctatc ttctaaagaa tggaaggtga taattgctgc cctgcctcct ggctttacca
     4261 aattgtttaa ctgcagcaac atttctttca aagcatcaat ttcttcattt acttcaattc
     4321 gcaatgcctg aaacacctga gccaagtatt tattaggatt tcctttcacc acagcactta
     4381 ttagcatttt aaactgttct attgtttgta aaggctgctt agttctgttt tgaacaatgt
     4441 gctttgccaa agtttttgca ttagtaacct cgccaaattg ttcaaatatt ttatgcagct
     4501 gctgttcggt aaacgattga ataactttag cagcagtttt attttcatta gtgttcattc
     4561 tcatatctaa agggccatca aaccttgttg aaaagcctct ttcgccaaca tcaaattgat
     4621 gagagcttac acccaaatcc gctaaaacac catctacctg ttgcacatta tacaatctta
     4681 aa
//
LOCUS       DAOQHP010000155         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10694_length_4662_cov_3.278706, whole genome shotgun sequence.
ACCESSION   DAOQHP010000155 DAOQHP010000000
VERSION     DAOQHP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10694_length_4662_cov_3.278706"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLY81_05970"
     CDS             <1..691
                     /locus_tag="PLY81_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNF29808.1"
                     /translation="KMYRVENISSEMSFLEMFDVLNERLIAEGDEPIAFDHDCREGIC
                     GMCSMYINGKPHGPWQGNTTCQLHMRAFKDGDTIVVEPWRSNAFPVIKDLVVDRTAFD
                     RIIQAGGYISVNTGNAVDGNALPINKDKADASFAAAMCIGCGACVAACKNSSAMLFLS
                     AKVSHLALLPQGEPERNSRVLSMVAQMDKEGFGSCTNTGACEATCPKEISISYIAKLN
                     QEYLCASLTAE"
     gene            complement(899..1063)
                     /locus_tag="PLY81_05975"
     CDS             complement(899..1063)
                     /locus_tag="PLY81_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29809.1"
                     /translation="MQQFETFFKPILKDLKVELTDEFDRNFERKAFFYFDICDKDLLQ
                     CADAVIRLRA"
     gene            1082..1312
                     /locus_tag="PLY81_05980"
     CDS             1082..1312
                     /locus_tag="PLY81_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29810.1"
                     /translation="MDTIFDYNPTEQELVGLFYKTENEYKQNTSNDMIIADLAILFAD
                     RNQPQKANEYWAKIPELHQEFLLGFDDVLIPQ"
     gene            complement(1456..2064)
                     /locus_tag="PLY81_05985"
     CDS             complement(1456..2064)
                     /locus_tag="PLY81_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29811.1"
                     /translation="MFRYNPGKQGVIFPEKHPYYGISKNALEVVKAMQQVEQSKRIDL
                     NKYIKNNVTAKIVRDVMLEYSNNFPENFLNGLEKIKFTSSQSYMMQHTINVNNITRKR
                     VGKSTISISNQTLRNGYNPAKEFIDGLASIKQGRELTFNQEYAFESMWHEILHAKTAS
                     QHHKLSNRQKEHMETVNQFVARHTYPKFIEAFDGKASNQMKY"
     gene            complement(2128..2361)
                     /locus_tag="PLY81_05990"
     CDS             complement(2128..2361)
                     /locus_tag="PLY81_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNF29812.1"
                     /translation="MVILIQHIAAAQSAAQYHEYMQDAERYNLQIRTAGDDKVRASHA
                     ELNGITLPANHEYWDTTWTPFDWRCRCRIIQVL"
     gene            complement(2484..2702)
                     /locus_tag="PLY81_05995"
     CDS             complement(2484..2702)
                     /locus_tag="PLY81_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29813.1"
                     /translation="MGILFGIISTIGIGVILFYCFLLLLRMAGFVIAMPVIIIQLCSK
                     KENRRKIWLPLIAITIFSFACWLWFTDH"
     gene            2721..3023
                     /locus_tag="PLY81_06000"
     CDS             2721..3023
                     /locus_tag="PLY81_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29814.1"
                     /translation="MANILEYILSLKDQTKKTLANIGINSDRAYNKFNALKQQANEVS
                     RVMKVFGGSVGALRQKLELLKNERDWLPAKEINTIRAYNSEIKKLTAQITKFETIK"
     gene            3207..>4662
                     /locus_tag="PLY81_06005"
     CDS             3207..>4662
                     /locus_tag="PLY81_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNF29815.1"
                     /translation="MKFLISCCLLLSLSISFAQKTITTKFELSKGTQTPTYFEIIDWW
                     KKLDNQSGKIKLLTMGNTDAGYPLHLAVISNNGDYNFNSIRKNNKRIIFINNGIHPGE
                     PDGIDASILLARDIVVDKQKLPDNIVLCIIPVYNIGGCLNRSKNFRVDQNGPEEFGFR
                     GNAQNLDLNRDMIKCDSKDARAFTEIFHLLDPDVFIDNHVSNGADYQHIMTLLTTQQS
                     KLGGVMGDYISKKFEPSIYSLMKQKGYDLIPYVNVWGNTPDKGWTEYWDSPRYASGYA
                     ALWHTFSFVPESHMLKTYEQRVKATYTLMQSFIEFTSANSKEIKVLREQSKQLAKSAV
                     DFPIKWSVDKSKFKEVNFFGYESGLKNSEVSGMPRLFYDKTKPYQKTVKIYNYFTPKE
                     FVKKPSAYIIPQGWWKVIELLQLNKIEMISLKKDTIIEVEVYKIEDYKTSSKQYEMHH
                     LNSEVKYTTSIKKVNFRKGDWYIPLNQVANRFLIE"
BASE COUNT         1615 a          694 c          794 g         1559 t
ORIGIN      
        1 taagatgtac agagtagaaa acatttcatc ggaaatgagt tttttagaaa tgtttgatgt
       61 attgaatgaa aggttaattg cagaaggaga tgaaccaata gcatttgacc atgattgccg
      121 agaaggtatt tgtggaatgt gctcaatgta tattaatggt aaaccacatg gcccatggca
      181 aggaaataca acctgccaat tacacatgag ggcttttaaa gatggagaca caattgttgt
      241 agaaccttgg agaagtaatg cttttccggt tattaaagat ttagttgtgg atagaactgc
      301 ttttgataga attatacaag cagggggtta tatatctgtt aatactggta atgcagtgga
      361 tggaaatgct ttgccaataa ataaagataa ggcagatgca agctttgctg ctgccatgtg
      421 tataggttgt ggagcttgtg tggctgcttg taaaaacagc agtgcaatgt tatttttatc
      481 agccaaagta tctcatttag ctttattacc tcaaggagag ccagaaagaa atagtagagt
      541 tttaagtatg gtggcacaaa tggataaaga aggctttgga agttgtacca atacaggagc
      601 ttgtgaagca acttgcccta aagaaatttc tatcagctat attgcaaaac taaatcaaga
      661 gtatttatgt gctagtttaa ctgctgaata attttatatt acttgttgtt tattaaataa
      721 attatttaaa aggtatttca atttgaaatg ccttttttat ttttaatgaa taattacatt
      781 gtatgaaatt tttaattagc tatggtctta ttattttttt ataatcattc catctaaata
      841 cacctacatt cgtaaaatga aaatatattt catcaaattt tttctgttga aataaatatt
      901 atgctctcaa tcgtattaca gcatctgcac attgcagtaa gtctttatca caaatatcaa
      961 aataaaaaaa agccttacgt tcaaaatttc tgtcaaactc atcggtaagc tctactttta
     1021 agtcttttaa aataggttta aaaaaagttt caaattgttg catttttagt aattttactt
     1081 tatggatact atttttgatt ataacccaac agagcaagaa ctcgttggct tattctataa
     1141 aacggaaaat gagtataagc aaaacacaag caatgatatg attattgccg atttggctat
     1201 actgtttgca gatcgtaatc agccacaaaa agccaatgaa tattgggcta aaatacccga
     1261 actacaccaa gagtttttac ttggttttga tgatgtatta atcccccaat aactcatcta
     1321 tcttttgccc caggctgctg taatctttca ttaaatgtgg ctctaaaaaa tcaattgcct
     1381 tcttttcgtc aattcctttt ttagccaact tgtttctaaa tttggttatc cattctttat
     1441 agccatatcc attatctaat atttcatttg gttggaggct ttgccatcaa atgcttcaat
     1501 aaattttggg taggtatgcc ttgccacaaa ttggtttact gtttccatgt gttctttttg
     1561 cctgttactt aatttgtggt gctggcttgc agttttggca tgtagtattt catgccacat
     1621 tgattcaaaa gcatactctt ggttaaatgt tagctcacgt ccttgtttaa ttgaagccag
     1681 cccatcaata aactctttag ctgggttata accatttctc aatgtttgat tgctaattga
     1741 aatagtgctt ttgcctacac gttttcttgt tatattattt acattaatgg tgtgttgcat
     1801 catataactt tgagaggatg taaattttat tttttctaac ccatttaaaa agttttcagg
     1861 aaagttatta gaatattcca acatcacatc tctaacaatt ttagcagtta cattgttttt
     1921 tatgtatttg tttaaatcaa ttcttttgct ttgctccact tgctgcatag ccttaactac
     1981 ctccaaagca tttttactaa taccatagta agggtgtttt tcaggaaaaa taacaccctg
     2041 tttaccaggg ttgtacctaa acacttcggg tatggcttgc tgtgctgctg cattggcaat
     2101 gccatcatcg gtggtagtat atttgcttta taatacctgt attattctgc atctacaacg
     2161 ccagtcaaag ggtgtccatg tggtgtccca atattcgtgg ttggcaggta gtgtaatgcc
     2221 atttagctct gcatggcttg ctcttacttt atcatctcct gctgtgcgta tttgcaaatt
     2281 atacctctcg gcatcttgca tgtattcatg gtattgtgca gcactttgtg cagcagcaat
     2341 gtgttggatt agaataacca atgaacaagc ttatgtacaa atgcacatat aaaatctaga
     2401 cagccttagc aatgaacaat gggcaatgcg aataaaagaa ttggagtgga ttagaaagaa
     2461 agaagcagga aaataatcta atgctaatgg tcggtaaacc ataaccagca ggcaaatgaa
     2521 aatatagtaa ttgctattag tggcaaccat atttttcgtc tgttttcctt tttagaacat
     2581 agctgtataa taataactgg catggcaatt acaaaacctg ccatacgcaa aagtagtaaa
     2641 aagcaataaa acagaattac acctatacca attgttgaaa ttattccaaa taatattccc
     2701 atagtgtaaa agtacaaatt ttggcaaaca ttcttgaata catattaagc cttaaagacc
     2761 aaacaaaaaa aacgttggca aatattggca ttaatagcga cagagcatat aataaattta
     2821 atgctttgaa acaacaagcc aatgaagtaa gccgagtgat gaaggtattt ggtggcagcg
     2881 ttggagcatt aaggcaaaag ttagaattac ttaaaaatga aagagattgg ctgcctgcaa
     2941 aagaaataaa taccatacgt gcctataaca gtgaaataaa aaagcttaca gcccaaataa
     3001 ccaaatttga aactattaaa tgagtaaaga gtcaacttgc cctaaagaaa tttctatcag
     3061 ctatatttca aaactaaatc aagagtattt atgtgctagt ttaactgctg aataatttta
     3121 tattacttgt tgtttattaa ataaattatt taaaaggtat ttcaatttga aatgcctttt
     3181 ttatttttaa tgaataaata cattgtatga aatttttaat tagctgttgc ttacttctaa
     3241 gtttatccat ctcatttgct caaaagacaa ttacaactaa gtttgaatta tcaaaaggca
     3301 ctcaaacgcc aacttatttt gagattatag attggtggaa aaaattagat aatcaatctg
     3361 gcaaaataaa attgctcaca atgggtaaca cagatgctgg gtatccatta catcttgcag
     3421 taattagcaa taatggtgat tataatttca attcaattcg taaaaataat aaaagaataa
     3481 ttttcattaa taatggtatt catccaggtg agccagatgg tattgatgca agtattttgc
     3541 ttgctagaga tatagttgta gataaacaaa aacttcctga taacatagta ctttgtatta
     3601 tacctgttta caatattggt ggttgcttaa accgaagtaa aaactttaga gtggatcaaa
     3661 atgggccaga ggaatttgga tttagaggca atgctcaaaa tttagattta aacagagata
     3721 tgataaaatg cgactctaaa gatgctaggg cttttacgga aatatttcat ttattagacc
     3781 ccgatgtttt tatagataat catgtaagta atggtgccga ctatcaacat ataatgacct
     3841 tgcttacaac acaacaatca aaacttggtg gtgtaatggg agattatata agcaaaaaat
     3901 ttgaaccttc tatttatagt ttaatgaagc aaaaaggata tgatttaata ccctatgtga
     3961 acgtttgggg aaatacacca gataaaggtt ggactgaata ttgggatagc ccaagatacg
     4021 ctagtggtta tgcagcactt tggcatactt tttcttttgt tccggagtct cacatgctta
     4081 aaacttatga acaaagagtt aaagcaactt atactttaat gcaatctttt attgaattta
     4141 catctgctaa tagtaaagaa attaaagtgc ttagagagca atcaaaacaa ttagcaaaat
     4201 ctgcagttga ttttccaatt aaatggagtg tagataaatc taaatttaaa gaagtaaatt
     4261 tctttggtta tgaatctggt ttaaaaaata gtgaggtttc gggcatgcca agattatttt
     4321 atgataaaac taaaccgtac caaaaaacag taaaaatata taattatttt actcccaaag
     4381 aatttgtaaa aaaaccttca gcttatataa ttccacaagg ctggtggaag gttattgaat
     4441 tattacaact gaataaaatt gaaatgatat ccttaaaaaa agatacaatt atagaggttg
     4501 aagtttataa aattgaggac tataaaacat catctaaaca gtatgaaatg catcatttaa
     4561 acagcgaagt aaaatataca accagcatta aaaaagtaaa ttttagaaaa ggagattggt
     4621 atattccact aaatcaggta gctaatcgtt ttttaataga aa
//
LOCUS       DAOQHP010000156         4651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10729_length_4651_cov_3.422106, whole genome shotgun sequence.
ACCESSION   DAOQHP010000156 DAOQHP010000000
VERSION     DAOQHP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10729_length_4651_cov_3.422106"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLY81_06010"
     CDS             <1..267
                     /locus_tag="PLY81_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29816.1"
                     /translation="YDLAEAWINFTKLPFVFAAWVANTNIEQEFMNAFNKANAYGIEN
                     IDKVVEDINYPFYNLQYYYTNNISYFLDEEKKKGLELFLQFLKQ"
     gene            complement(374..445)
                     /locus_tag="PLY81_06015"
     tRNA            complement(374..445)
                     /locus_tag="PLY81_06015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Glu,seq:ttc)
     gene            632..1225
                     /locus_tag="PLY81_06020"
     CDS             632..1225
                     /locus_tag="PLY81_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNF29817.1"
                     /translation="MRTNQLSLLVIGGIILLLGGWSCSKYNGLVKTDVEVQKVWGNVE
                     TNYQRRTDLYSSVIKTIEGSANFEKSTLKDVINARAAATQVKVDINDPESLAKYQAAQ
                     GQLQSAFGRLMAVAEAYPDLKTTKSFQDFQAQIEGTENRINVARQDYNKAVQNYNLKV
                     RTFPNNIFAGFFGFKSKEFYKADPGTEKAPEVNFNIK"
     gene            1234..1695
                     /locus_tag="PLY81_06025"
     CDS             1234..1695
                     /locus_tag="PLY81_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF29818.1"
                     /translation="MLPFFKKKPTNYFSEPEKQLIVNAIKQAETNTSGEIRVFIENKC
                     SYVDAIDRAKELFEKLKMFETEQRNGVLLYVALKDRQLAIFGDKGIYEKTGVEFWNNE
                     VNELLLFFNKLNYAEGIANVVIQIGNALKLHFPFNKSSDVNELKDEIVFGK"
     gene            1735..2517
                     /locus_tag="PLY81_06030"
     CDS             1735..2517
                     /locus_tag="PLY81_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF29819.1"
                     /translation="MKYFFSILFSLLVNISFAQKSIPKPNPPKLVNDYAGILDKHDAD
                     LLEKKLVALDDSTSNQIVVLTVKSLNGEILEDVAVNTFREWGIGNKKTNNGVLILIAL
                     EDKKVRIEVGYGLEGAIPDVIANDILKTYLAPAFKQGNYLDGINKAVDNITKAAAGEY
                     KVKRKKSNETSGKSLIIFIIILFVVLMIVGNSGRGGGGGNIMNRGGSNDIATNLFLLS
                     LLNSRSGRGNSGGGWGGGDWGGGGFGGFGGGSSGGGGASGGW"
     gene            2621..3343
                     /locus_tag="PLY81_06035"
     CDS             2621..3343
                     /locus_tag="PLY81_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNF29820.1"
                     /translation="MGRIFEVRKATMFARWDRMAKQFTRIGKEIAIAVKAGGTDPHTN
                     PALRRCMQNAKSVNMPKDRVEAAIKRAMGKEMENYEEILYEGYAPHGVAILVETATDN
                     HVRTVANVKSHFNKGGGSLGNSGSVSFQFKKMGVFKLKADGLNVDDLELELIDYGLEE
                     IGDSTDDDGNTILVMRCEFTDFGNLQKILEEKNITPITAELEWIPSSTVPVNDAQAED
                     ISKLIERLEQDDDVNKVFHNMG"
     gene            3608..>4651
                     /locus_tag="PLY81_06040"
     CDS             3608..>4651
                     /locus_tag="PLY81_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29821.1"
                     /translation="MKKIILMLGFKQQKPTVPNNYLIAEFLLVILFILISLLFPFISS
                     AQKGKDGAESYTTAGTRILNRYSALAVSAGSGSTNIEVASVSDLSGAFSFVNSVNPYA
                     SNDLATGDLVFIIQMQGADIDVTNTSSYGDITAYNGVGDYEFKTVLSVSGNRIYFCDP
                     LVNSYYVGERKRAQVIRVPRLTTLNNSGAASRIITGSAWNGTTGGVCVIETSGNLTFS
                     SNATITANGIGFRKGVDPNRTSSITSGLGTNNTTYRNTLTTNDAGKGEGIAGNSSDYD
                     TYLSGAQGRGAAANGGGGGNGHNCGGGGGSNAGSLSGWNGTGTKPAGYNAAWNLEGAG
                     FATNVSPGGGRGGY"
BASE COUNT         1668 a          617 c          909 g         1457 t
ORIGIN      
        1 tatgatttag cagaggcctg gataaatttt actaaactgc catttgtatt tgctgcatgg
       61 gtggcaaata caaatattga acaagagttt atgaatgctt ttaacaaagc caatgcttac
      121 ggtatagaaa atatagataa agtagttgaa gacattaatt atccttttta caacttacaa
      181 tattactata ctaacaatat cagctatttt ttagatgaag aaaagaaaaa agggttagaa
      241 ctatttctgc aatttttaaa acaataatgg tttaatattt tacctatggt tgttttaatg
      301 cattccagaa aaaacaataa gtgatttttt gaaaatataa aagccatctg caagtgcaaa
      361 tggcttttat tagtagcccg taggggaatc gaacccctct ttcctccgtg aaagggagat
      421 gtcctagccg atagacgaac gggccatatt ttttgtcaat cgggctgcaa atatagaagc
      481 acaaaaatta gtagccaaaa ttttttaaaa tattttaata ttaatttatt gccaaaatca
      541 taatttattg ttagtacagt aaaatgtaac taattactat gtatttttat atttaattta
      601 caattcaaac aataaactta aaaaaataaa aatgagaaca aatcaacttt cattacttgt
      661 aattggtggt attatattgt tattaggtgg ctggagctgt tcaaaatata atggattggt
      721 aaaaacagat gtagaagtgc aaaaagtttg gggtaacgta gaaacgaact accaaagaag
      781 aaccgactta tatagtagtg taattaaaac tattgaaggg tctgctaatt tcgaaaaaag
      841 cacactaaaa gatgtaatca atgcacgtgc tgctgctaca caagttaagg tagatattaa
      901 cgatcctgag tcattagcaa aataccaggc agcacaaggg caattacaaa gtgcatttgg
      961 cagactaatg gctgtagcag aggcttatcc tgatttaaaa actaccaaat cgtttcaaga
     1021 ttttcaggct caaatagaag gcacagaaaa tagaattaat gtagcaagac aagattataa
     1081 caaagctgtt caaaactata atctaaaagt aagaactttc cctaataata tttttgcagg
     1141 tttctttggc tttaaatcta aagagtttta taaagcagat cctggcacag aaaaagcacc
     1201 tgaggtaaat ttcaatatca agtaattttt gttatgttac ccttctttaa aaagaaacca
     1261 actaattatt tttctgaacc tgaaaagcaa cttatagtaa atgctataaa acaagcagaa
     1321 acaaacacta gtggcgagat aagggttttt attgaaaaca aatgcagtta tgtagatgct
     1381 attgatagag caaaagaatt gtttgagaaa ttaaaaatgt ttgaaacaga acaacgcaat
     1441 ggcgttttgt tatatgttgc cctaaaagat agacagttag caatttttgg agataaggga
     1501 atttatgaaa aaacaggagt tgagttttgg aataatgaag taaatgaact tttacttttt
     1561 tttaataaat taaactatgc cgaaggtata gctaatgtag ttattcaaat tggcaatgca
     1621 ctaaaactac attttccttt taataaatct tctgatgtga atgaactgaa agacgaaatt
     1681 gtttttggaa agtaattttt tatacaaaaa ataaatggct actgtaaact attcatgaaa
     1741 tattttttct ccatactatt ctccttactt gttaatatat cttttgcaca aaaaagcatt
     1801 cctaaaccta atccaccaaa attagtaaat gattatgcag ggatattaga taaacacgat
     1861 gccgatttat tagaaaaaaa attagttgca ctagatgata gtaccagcaa tcaaattgtt
     1921 gttttaactg taaaatcgct aaacggagaa attttagaag atgtagcagt aaacacattt
     1981 agagaatggg gtattggcaa taaaaaaaca aataatgggg ttttaatact cattgcttta
     2041 gaagataaaa aagttagaat tgaagtaggc tatggtttag aaggtgcaat accagatgtt
     2101 attgctaacg acatattaaa aacatatctt gctcctgcat ttaagcaagg caattattta
     2161 gatggtataa ataaagctgt agataatatt accaaagcag ctgctggcga atacaaagta
     2221 aaacgcaaaa aaagtaacga aacaagtgga aaaagcctta ttatttttat aataatttta
     2281 tttgttgtat taatgattgt aggcaatagt ggaagaggag gaggaggagg aaatataatg
     2341 aatagaggag gaagtaatga tattgctaca aatttatttt tgttaagcct tttaaattca
     2401 cgttctggaa gaggaaacag tggaggaggt tggggaggag gagattgggg tggtggaggt
     2461 tttggtggtt ttggaggtgg tagttctgga ggaggtggag ctagcggtgg ttggtaaaaa
     2521 attaaacaca agatttattt ttttaagcaa gctattctaa aatttagctt gctttcttat
     2581 ttttgcactc ctttaataaa acatttttta gaaatattgt atgggaagaa tttttgaggt
     2641 aagaaaagca accatgtttg cccgttggga cagaatggct aaacaattta ctcgaattgg
     2701 taaagaaata gcaatagcag ttaaagctgg tggaacagat ccgcacacta atcctgcatt
     2761 gcgtaggtgt atgcaaaatg ctaaaagtgt taatatgcct aaagacagag tagaagctgc
     2821 aattaaaaga gctatgggaa aggaaatgga gaactacgaa gaaattttat acgaaggata
     2881 tgcaccacat ggtgtagcta ttttagtaga aaccgctacc gataatcatg taagaactgt
     2941 agctaatgta aaaagtcatt tcaacaaagg tggtggtagc ttgggtaata gcggtagtgt
     3001 aagttttcag tttaaaaaaa tgggtgtttt taaattaaaa gcagatggct taaatgtgga
     3061 tgatctggaa cttgaactta ttgattacgg attagaggaa attggagaca gcaccgatga
     3121 tgatggaaat actattttag taatgcgttg cgagttcaca gattttggta acctacaaaa
     3181 aattttagaa gaaaaaaata ttacaccaat tacagccgaa ctagaatgga ttccttcttc
     3241 cactgttcct gtaaatgatg cccaagcaga agatattagt aaacttatag aaagattaga
     3301 acaggatgac gatgtaaata aagtgttcca taatatgggc taaactacaa tcaatcattt
     3361 aatatattga tatataccta cctaaataat tttgatataa acttagagtt gaaagtaatt
     3421 tttttgtaaa ttattaatta aagtaactta ctgtaaatag ttgataatta gccatatatg
     3481 tagttagaaa gtaccccaat cggacattaa ttcttaaatt atgttaatag tatcttttta
     3541 aaagcaatat gttggcattg tcttacgtta taccatagca atcattctat ttagaagccg
     3601 gaaagctatg aaaaaaatta tactaatgct tggttttaag cagcaaaagc ctactgttcc
     3661 aaacaactac ttgatagccg aatttctttt ggttatttta tttatcctta tttctctact
     3721 atttcccttt atttcaagtg ctcaaaaagg aaaagatggt gcagagagct atactactgc
     3781 cggtactcgt attttaaaca ggtattctgc attagctgtt tcagcgggtt caggttctac
     3841 aaatattgaa gtggcatctg tttcagattt aagtggtgct ttcagttttg ttaattcagt
     3901 aaacccatac gcttctaacg atttggctac aggagattta gtttttatca ttcaaatgca
     3961 aggagccgac attgatgtta ctaacacatc ttcttatggt gatataacag cttataatgg
     4021 ggtaggtgat tatgaattta aaacagtttt aagtgtttca ggcaatagaa tttatttctg
     4081 cgacccttta gtaaattctt actatgttgg tgaaagaaaa agagcacagg taattcgtgt
     4141 tccaagatta acaacattaa acaattcagg tgcagctagt agaataataa caggaagtgc
     4201 atggaatggt acaacaggtg gggtatgtgt aattgaaaca tcgggtaact taactttttc
     4261 ttctaatgct acaattactg caaatggtat tggttttaga aagggtgttg accctaatag
     4321 aacaagttct ataacttctg ggcttggtac aaataataca acttatagaa atactttaac
     4381 aacaaatgat gctggtaaag gtgaaggaat tgccggaaat tcatctgatt acgataccta
     4441 tttgtctggt gcacaaggtc gtggtgcagc agcaaatgga ggaggtggtg gaaatggtca
     4501 taattgtggc ggtggtggtg gctctaatgc tggctcatta tctggttgga atggcacagg
     4561 taccaaacct gccggatata atgctgcttg gaatttagaa ggagcaggct ttgcaactaa
     4621 tgtttcgcca ggtggtggtc gtggaggtta t
//
LOCUS       DAOQHP010000157         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10890_length_4600_cov_4.091749, whole genome shotgun sequence.
ACCESSION   DAOQHP010000157 DAOQHP010000000
VERSION     DAOQHP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_4600_cov_4.091749"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2783
                     /locus_tag="PLY81_06045"
     CDS             <1..2783
                     /locus_tag="PLY81_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29822.1"
                     /translation="LYHNIETTEDSFTYTAIIESTLRRSSQGLVEAYKQSRIDKKNNL
                     LILVDQFEEIFRFSRVEKDAKEGKRDSIAFINLLLKASEQKEFPIYVVFTMRSDFLGD
                     CTEFRGLPEAINEGQYLVPRMTRDERREAITGPVAVGGATITPQLLNQLLNDVGDNPD
                     QLPILQHALMRTWNIWSSKSNNNTPIDIDAYEEIGTMSNALSQHAEEAYNDLNTDRKK
                     QICESLFKSLTDRGSDTRGIRRPTKLSEICKLANASSNEVIEVIDVFRANGRSFLMPP
                     SEVAINEDTIIDISHESLMRGWNRLLNWVEEENQSAEIYLRLCEAANLYELGKGGLWR
                     DPELQLAWKWKEEAKPNVTWASRYNNYFEKAILFLDYCKQQSELAIQHKEKMHKRRLK
                     RARLIAIFISCIAIAALLLAIFSFDQRNKANQQKIIALNKTEEAEKERKNALAQKHLA
                     VENANEAEKQKQKAEENATEAINQKTEAEKQKQKAEENAIEANKQKKNAEEKTIEAEK
                     QKQKAEENATEANKQKKKAEEKTIEAEKQRKAAEEQRKISTKLKDLAEAKNLANLSLQ
                     KFREGNKTESKKLAINAFLLNKENDGPKQNAEIYTALNTNWADAIKNKNQATYHKYAV
                     KNIIGKSNNNIVFTADEAGFIYTAKEENGILQTIGKPILLKEDIRALAVTKDNTKLFV
                     ATANGNCFIFNCSSNAQTTLTNNFKINGIAKSIAVINNQTFIIITDKEILKVTTDKAS
                     IMQASLQRSGLTSLVVGDKLNKIFVAENNKLFIYNTYNDLVNNQISNTYTLPSKITSL
                     AINENENFIAAGTYDGTLWLKETNSHNNPTTATIHKSSINCIQFKKLKNGVWQIATGS
                     ADHTIKLMDVQQLVQHKQTEDIITLSDGGHTKWVYGIWYSNNGEHLYSCSEDKKIIGW
                     KTTMDGLYKALQ"
     gene            3103..>4600
                     /locus_tag="PLY81_06050"
     CDS             3103..>4600
                     /locus_tag="PLY81_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29823.1"
                     /translation="MLVVLLISVSTQAQSTSYNSNTTPINGANCTAFGFGALTANTTG
                     FNNVANGSYSLFSNTIGNYNTANGTSALYSNTTGWYNTANGNQSLFNNTFGESNTANG
                     YLSLFNNTTGSANVANGSFALYSNTTGWYNTANGVGSLYSNTTGSANVANGYKSLYSN
                     STGSANVANGNYSLYSNTNGERNVANGDSSLYYNTTGYYNVANGYQSLFNNTFGFYNT
                     ANGYQSLLNNTFGNYNTANGGGSLYSNTTGIGNVANGYQSLYSNTAGHLNVANGSSSL
                     FSNTTGYDNVANGSGSLFSNTTGYNNVANGSGSLFSNTTGYDNVANGRNSLFSNTTGY
                     NNVANGIVSLSSNTTGNYNTANGSYSLAANTTGIDNVANGSYSLFFNTTSSGNVANGY
                     KSLYSNTTGSTNTANGFQALYYNSTGVNNVAIGGMAGFNSLGSNNVYIGSQAGQTETG
                     SDKLYISNGSTNAIIYGDFATHQLLLGVPNATGYVFKGNRTLNVMGGIL"
BASE COUNT         1768 a          777 c          772 g         1283 t
ORIGIN      
        1 tattatatca taacattgaa acaaccgaag acagctttac ttatactgct attattgaaa
       61 gcactttaag aagaagcagc caaggcttgg tagaagccta taaacaaagt agaattgata
      121 aaaaaaataa cctgctaata ctggtagatc aatttgaaga aatttttaga tttagcaggg
      181 ttgagaaaga tgctaaagaa ggaaaaagag actccattgc atttattaat ttacttttaa
      241 aagcatccga acaaaaagaa tttcctattt atgttgtatt taccatgcgg tctgactttt
      301 taggagattg taccgagttt agaggattgc cagaagcgat aaatgaaggg caatatcttg
      361 tacctagaat gacaagagac gaaagaagag aagcaattac aggaccagta gctgttggtg
      421 gggcaactat aacccctcaa ctattaaatc aactgttaaa tgatgttggc gataacccag
      481 accaattacc cattttgcaa catgcattaa tgcgaacatg gaatatttgg tcttccaaaa
      541 gtaataataa tacgcctatt gatattgatg cttatgaaga aataggaaca atgtctaatg
      601 ctttatctca acatgcagaa gaagcatata acgatttaaa cacagataga aaaaaacaaa
      661 tttgcgaaag cctttttaaa tcattaacag atagaggtag cgatacaaga ggtataagaa
      721 ggccaaccaa actatcagaa atatgtaaac ttgccaatgc cagctctaac gaagtgatag
      781 aagtaattga tgtttttaga gcaaatggta gaagcttttt aatgccacca agcgaagtag
      841 caattaacga agataccatc attgatattt ctcacgaaag tttaatgaga ggttggaaca
      901 gattattaaa ttgggtagaa gaagaaaacc aatctgccga aatttattta cgcctttgtg
      961 aggctgctaa tttgtatgaa ctgggaaaag gaggcttatg gagagaccca gaattacaac
     1021 ttgcctggaa atggaaagaa gaagctaaac ctaatgtaac atgggcatct cgttacaata
     1081 actacttcga aaaagcaatt ctttttttag attattgtaa acaacaaagt gaattagcca
     1141 ttcagcataa agaaaaaatg cacaaaagaa ggttaaaaag ggcaaggctg attgctattt
     1201 ttatttcctg tattgccata gctgctttgt tattggcaat attttctttt gaccaacgta
     1261 ataaagctaa ccaacaaaaa ataattgcat taaataaaac agaggaagca gagaaagaaa
     1321 gaaaaaatgc acttgctcaa aaacatttag ctgttgaaaa tgcaaacgaa gccgaaaaac
     1381 aaaaacaaaa agccgaagaa aatgcaacgg aagcaataaa ccaaaaaaca gaagccgaaa
     1441 aacaaaaaca aaaagccgaa gaaaatgcaa tagaagctaa taagcaaaaa aagaatgcag
     1501 aagaaaaaac catagaagcc gaaaaacaaa aacaaaaagc cgaagaaaat gcaacagagg
     1561 ctaataagca aaaaaagaaa gcagaagaaa aaaccataga agccgaaaag caaagaaaag
     1621 cagcagaaga acaaagaaaa atttcaacta aattaaaaga tttagccgaa gccaaaaatc
     1681 ttgccaacct ttctttacaa aaatttagag aaggtaataa aacagagagt aaaaagcttg
     1741 ctatcaacgc ctttttgctt aataaagaaa atgatgggcc taaacaaaat gcagaaattt
     1801 ataccgcact aaacaccaat tgggccgatg caataaaaaa taaaaaccaa gcaacttacc
     1861 ataaatatgc agtaaaaaat ataataggca aatcaaataa taatattgtt tttactgcag
     1921 atgaagcagg ctttatatat acagcaaaag aagaaaacgg aattttacaa accattggta
     1981 aaccaatttt acttaaagaa gatattaggg ctttggcagt aactaaagat aatacaaaac
     2041 tttttgtagc cactgctaat ggcaattgct ttatttttaa ttgtagtagt aacgcacaaa
     2101 ctacactcac caataacttt aaaataaatg gtattgctaa aagtatagca gtaatcaata
     2161 atcaaacatt tattattatt accgataaag aaattttaaa agttactacc gacaaagcct
     2221 ctattatgca agcaagttta caacgcagtg gattaacctc attagttgta ggcgataaat
     2281 tgaacaagat ttttgtggca gaaaataata aactttttat ttataacaca tacaacgatc
     2341 ttgtaaataa ccaaataagt aacacttata ccttaccatc taaaattaca agtttggcta
     2401 taaacgaaaa cgaaaatttt atagctgctg gcacttacga cggaacttta tggttaaaag
     2461 aaaccaactc acataataac ccaacaactg ccaccataca caaatcaagc attaactgta
     2521 ttcagtttaa aaaattaaag aacggagttt ggcaaattgc tacaggtagt gcagaccata
     2581 ctataaaact tatggatgta caacagttgg tacaacacaa acaaaccgaa gatattatta
     2641 ccttaagcga tggtggccat acaaaatggg tttatggtat atggtatagc aacaatggag
     2701 aacatcttta ctcttgcagc gaagataaaa aaattattgg ctggaaaaca actatggatg
     2761 ggctttacaa agctttacaa taattattaa actattagca aaaacacttt taaaacgttt
     2821 taaagtttta acgaagtaat tatttaaaac attactactg ttttgctttt ttactacaaa
     2881 caatatacat acgcaataag cctgtaatgc caatactatt acagaggcag aaaattatta
     2941 ttatagttta gttataaggg tttttaggca tatttccaaa aaatgcctgt cacatgatat
     3001 ttattatttt aaaaataatg actactatac tttagtataa taattcttaa ccaaaaaatt
     3061 acttttatga aaacaataaa tttattatag ggtattacct ctatgttggt ggtattactt
     3121 attagtgtaa gcacacaagc acaaagtaca tcttacaata gcaacacaac cccaattaat
     3181 ggagcaaatt gtactgcatt tggttttggg gctttaactg ccaacacaac cggatttaat
     3241 aatgttgcta atggtagcta ctctctattt tccaatacaa ttggaaatta caatactgct
     3301 aatggtacaa gtgctcttta ttctaataca actgggtggt acaatacagc taatggtaat
     3361 caatctctat ttaacaatac attcggagaa tccaacactg caaatggtta cctatctcta
     3421 tttaacaata caaccggtag tgccaatgtt gctaatggta gttttgccct ttattccaat
     3481 acaactgggt ggtacaatac agctaatggt gtaggctctc tatattccaa tacaaccggt
     3541 agtgccaatg ttgcaaatgg ttacaaatct ctatactcca attcaaccgg tagtgccaat
     3601 gttgctaatg gtaattactc tctatattcc aatacaaacg gagaacgcaa tgttgcaaac
     3661 ggtgattcct ctctatatta caatacaacc ggatattaca atgttgcaaa tggttaccaa
     3721 tctctattta acaatacatt cggattttac aacaccgcaa atggttacca atctctattg
     3781 aacaatacat tcggaaatta caacactgca aatggcggcg gctctctata ttccaacaca
     3841 accggaatag gcaatgttgc taatggttac caatctctat attccaatac tgctgggcat
     3901 ttaaatgttg ctaatggtag tagctctctt ttttccaata caacgggata tgataatgtt
     3961 gcaaatggtt caggctctct tttttccaat acaacgggat ataacaatgt tgcaaatggt
     4021 tcaggctctc ttttttccaa tacaacggga tatgataatg ttgctaacgg acgcaattct
     4081 cttttttcca atacaacggg atataacaat gttgctaatg gcatcgtctc tctatcttcc
     4141 aatacaacgg gaaattacaa caccgcaaat ggtagctact ctctagctgc caatacaacc
     4201 ggaatagaca atgttgctaa tggtagttac tctctatttt tcaatactac tagttcagga
     4261 aatgttgcta atggttacaa atcactttat tccaatacaa caggctctac caacacagca
     4321 aacggttttc aggctcttta ctataattct actggggtta ataatgttgc tattggtggt
     4381 atggcaggct ttaacagcct tggcagcaac aatgtgtata ttggtagcca ggcaggacaa
     4441 acagaaacag gcagcgataa attgtacatt agtaatggta gtaccaatgc cattatttat
     4501 ggcgattttg ctacgcacca gcttttattg ggtgtgccaa atgctacagg gtatgttttt
     4561 aaaggtaaca gaactttaaa tgttatgggt ggtattctta
//
LOCUS       DAOQHP010000158         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10910_length_4596_cov_3.623431, whole genome shotgun sequence.
ACCESSION   DAOQHP010000158 DAOQHP010000000
VERSION     DAOQHP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10910_length_4596_cov_3.623431"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLY81_06055"
     CDS             complement(<1..453)
                     /locus_tag="PLY81_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF29824.1"
                     /translation="MKNISVIGAGTMGNGIAHVFAQNGFTVNLIDVNAAQLEKAIATI
                     TKNMDRQIAKGSFTEEQKTTALQNLTTYISIEEGVANAELVVEAATENIELKLKIFKQ
                     IDAAAPVGCILASNTSSISITKIASATKRPHLVIGMHFMNPVPVMKLVE"
     gene            complement(517..1173)
                     /locus_tag="PLY81_06060"
     CDS             complement(517..1173)
                     /locus_tag="PLY81_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF29825.1"
                     /translation="MMKLIVHKLFFAIVIGLFCTKISTAQRYVSQDSVVQLYGVIMSA
                     DSLRAISNASIIVVNKGRGTISNSDGVFSIAVLKGDRITFSCIGFKDKTIDIPHNLKE
                     NQYSVIQLLVNDTAYLPATIIRSRPSREQFERDFVNTPIPDDAYEIARQNTDVAKRRV
                     LINSLPADGREAVNYQMRQQANKYYYAGQIPPMNILNPMAWAEFIKSWKRGDYKRKKN
                     "
     gene            1336..2430
                     /locus_tag="PLY81_06065"
     CDS             1336..2430
                     /locus_tag="PLY81_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNF29826.1"
                     /translation="MTVEAILDALRNVQEPDLGKDIVTLNMVKDIAIDGDKVSFTIVL
                     TTPACPMKDKMRMASENAVKLLVNKDAKVIVNFTAITSSKRLQGDKILGGVKNIIAVV
                     SGKGGVGKSTVSANLALALSSAGATVGLMDADIYGPSVPIMFGVRGQRPMMKEVDGKG
                     LIVPLEKYGIKLISIGLLVDEKNAVVWRGPMVSNAIRQFVTDVDWGELDYLVIDMPPG
                     TGDIHLTLMQTVPVTGVVIVSTPQKVALADAKKGIAMFGQSQINVPILGLVENMAYFT
                     PAELPNNKYYLFGKEGGKNLADEYDLNFLGQIPLVQSIMEGGDEGVPAMAGNDEVSKE
                     AFRNLTASIVRNIAIRNANIPATKIVEVKA"
     gene            complement(2507..3850)
                     /locus_tag="PLY81_06070"
     CDS             complement(2507..3850)
                     /locus_tag="PLY81_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF29827.1"
                     /translation="MNKILIIARREFLSRVQKKTFLLTTIGLPILIFGIYALIIYFAV
                     KTTDNFTIAIADEANIFEGKLAKKNDGEINFIHVQNETPETLKKQIEAKKINAFVFIP
                     KTFDITGSTDSIQVISDKSVGLITREKIIGKLNNVIEEKKMLGSLHITKTQLDSLQKT
                     NEIVFTTTKGVEDTSKKAGISYGVGFVSGFLIYIILFIYGTMVMRGVMEEKTNRIAEV
                     IVSSVKPFQLMMGKIFGIGSVGLVQFLIWIILIIGLQFLLPIIFPDMLTQMQAQPMQP
                     GIGAAASTMSQENTAMVGIMNGIGQVNWLLIVGCFLFYFLGGYFLYSSLFAAVGSTVN
                     EDPQDAQSLMLPITMPIIFGMVIMMKAVNDPNSSLAIFGSIFPLTSPIVMMARIAHGV
                     PEGVSILQLLISMACLIVGFVATTWLAGKIYRTGILMYGKKVNWKTIGSWILRKN"
     gene            complement(3882..>4596)
                     /locus_tag="PLY81_06075"
     CDS             complement(3882..>4596)
                     /locus_tag="PLY81_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HNF29828.1"
                     /translation="KIGYMPEERGLYKKMKIGEQALYLAQLKGMSKKDAMERIIFWFK
                     KLEMESWWNKKVEDLSKGMSQKLQFVTTVLHEPKLIILDEPFSGLDPINANLIKDEIY
                     GLAQRGSTVIFSTHRMEQVEEICDHIVLVNLGKKILDGTVQDIKQQFKKNKFSITLQN
                     EPTNTLSNVFTIDAQNNNSLTVSINNGYKSNDVLKHFIDTNQAIESFNEILPGLNEIF
                     ISLVEGTKAKTRAFEAVTV"
BASE COUNT         1497 a          743 c          707 g         1649 t
ORIGIN      
        1 ctctactaat ttcattacag gaactgggtt cataaaatgc ataccaataa ctaaatgtgg
       61 tcttttagtt gccgaggcaa tctttgtaat agaaatagaa gaagtgttac tggctaaaat
      121 acaaccaact ggtgctgcag catctatttg tttaaatatt tttaatttta attctatatt
      181 ttctgttgca gcctcaacaa ctaattctgc atttgctact ccttcttcta tacttatata
      241 agtggttaag ttttgtaaag cagttgtttt ttgctcttcg gtaaagctac cttttgctat
      301 ttgtctgtcc atattttttg ttatggtagc aatagctttt tctaattgtg ctgcatttac
      361 gtcaataagg tttacagtaa aaccattttg tgcaaaaacg tgagcaatgc cattacccat
      421 tgttccagca ccaattacag aaatattttt catgtataaa agtattaggc agcgaaagta
      481 aaataaattt ggatagatga attttaatta agcagattaa tttttctttc gcttgtaatc
      541 gccccgtttc cagcttttaa taaactcggc ccaagccata ggatttaaaa tattcattgg
      601 gggtatttga ccagcataat aatatttatt tgcttgttgc ctcatttgat aatttacagc
      661 ttctcttcca tcggcgggca agctatttat taatactctt cttttagcaa catctgtatt
      721 ttgccttgca atttcataag catcatctgg tattggtgta tttacaaaat ctctttcaaa
      781 ctgttctctg cttggcctag aacgtataat tgtagcaggt aaataagctg tatcgtttac
      841 tagtaattga ataacactat attggttttc ttttaaattg tgtggtatat caatggtttt
      901 atctttaaac ccaatacaag aaaatgttat tctatcgcct tttaaaactg caatactaaa
      961 tacgccatca ctattactaa tagttcctct tcctttatta actacaataa tacttgcatt
     1021 actaattgct cttaaactat ctgctgacat gattacacca tacaattgta ccacactatc
     1081 ttgtgataca tatcgttgtg cagtagagat tttagtgcaa aacaacccta tcacaatagc
     1141 aaaaaataat ttatgtacaa ttaatttcat cattcaatta accaaaataa actaatgtat
     1201 aataagtttc aaataaaaat aattttaaaa tatttcttga ttcaaaactt caaaatgtaa
     1261 acttcaacaa agtaaacctc tgaaagtttt atcttcgcaa cttatataat tttttaacaa
     1321 aaactttcaa caataatgac tgtagaagca attttagatg ccttaagaaa tgtgcaagaa
     1381 ccagatttag gaaaagatat agtaacctta aatatggtaa aagatattgc aatagatgga
     1441 gataaagttt cttttaccat tgtacttact acacctgctt gccccatgaa agataaaatg
     1501 agaatggcca gcgaaaatgc agtaaaactt ttggtaaata aagatgccaa agtgattgtt
     1561 aatttcactg caataacttc atcaaaaagg ttacaaggag ataagatttt aggtggggtt
     1621 aaaaatatta ttgcagtggt aagtggtaaa ggtggtgttg gtaaaagtac agtaagtgca
     1681 aatttagccc ttgctttgtc aagtgcaggt gcaactgttg gtttaatgga tgctgatatt
     1741 tatgggccaa gtgtaccaat aatgtttggt gttagagggc aacgccccat gatgaaagaa
     1801 gtagatggta aaggtttaat tgtaccattg gaaaagtatg gaattaaatt aataagtatt
     1861 ggtttattgg ttgatgaaaa aaatgcagtt gtttggagag ggccaatggt aagcaatgct
     1921 ataagacaat ttgtaacaga tgtagattgg ggagaattgg attatttggt aattgatatg
     1981 ccacctggta caggtgatat tcatttaacc cttatgcaaa cagtgcctgt tactggcgtt
     2041 gttatagttt caacaccaca aaaagttgct ttagcagatg ccaaaaaagg aattgccatg
     2101 tttggccaat cgcaaattaa tgtacctatt ttggggttgg tagaaaatat ggcctatttt
     2161 actcctgcag aattaccaaa taataaatat tatttatttg gaaaagaagg tggaaagaat
     2221 ttggcggatg agtatgattt gaatttttta gggcaaattc cattagtaca atctataatg
     2281 gaaggtggcg atgaaggtgt gccagcaatg gctggtaatg atgaagtttc taaagaggca
     2341 tttagaaatt taactgcctc cattgttaga aacatagcaa ttagaaatgc aaatattcct
     2401 gctacaaaaa tagttgaggt gaaggcatag tattaacacc tctataaaat aaaagtggct
     2461 atattttata ttgaatgtag ccacttttat ttttactgaa cttgctttaa ttttttctta
     2521 aaatccaact accaatagtt ttccagttta cttttttccc atacattaaa atacctgttc
     2581 tataaatttt acctgctaac caagttgtgg caacaaaacc aacaattaaa caagccatgc
     2641 ttataagtag ctgtaatata gatacacctt ctggtacacc atgtgcaatt cttgccatca
     2701 taacaatagg agaagttaaa ggaaaaatgc taccaaaaat agctaagctg ctgttagggt
     2761 cgtttacagc tttcatcatt attaccattc caaaaataat aggcatagta atgggtaaca
     2821 ttaaactttg agcatcttgt gggtcttcat taactgtgct gcctactgct gcaaacaaag
     2881 aagaatacag aaaatatcct cctaaaaaat aaaataaaaa acagccaaca attagcaacc
     2941 aatttacttg cccaattcca ttcataatac caaccattgc tgtattttct tgagacattg
     3001 ttgaagctgc agctccaata cctggttgca tgggctgtgc ttgcatttga gttaacatat
     3061 caggaaaaat aatgggtaat aaaaattgta agccaataat taaaattatc caaattaaaa
     3121 attgaactag ccctactgag ccaattccaa atattttacc catcattaat tgaaaaggtt
     3181 ttacactact tacaattact tctgcaattc tatttgtttt ttcttccata acaccacgca
     3241 ttaccatagt gccataaata aaaagaataa tgtagataag aaagccagaa acaaatccta
     3301 ctccataact gatacctgct tttttactgg tatcttccac ccctttagtt gttgtaaaaa
     3361 caatttcatt tgttttttgc aaactatcta gctgtgtttt ggtaatatgc aagctaccca
     3421 acattttctt ttcttcaatt acattattta acttaccgat aattttttct cgggtaatta
     3481 aaccaacact tttgtcggat attacttgaa ttgaatctgt agaaccagtg atatcaaatg
     3541 tttttggaat aaatacaaaa gcattaattt tttttgcttc aatttgtttt tttaaagttt
     3601 ctggtgtttc attttgtaca tgaatgaaat tgatttctcc atcatttttc ttggctagct
     3661 ttccttcaaa aatattggct tcatctgcaa tggcaatggt aaaattgtcg gttgttttta
     3721 cagcgaaata tattattaat gcataaatgc caaatattaa aattggtaac cctattgttg
     3781 taagcaaaaa agtctttttt tgaactctac ttaaaaattc tcttcttgct ataattaata
     3841 ttttgttcat ggtttattta gataaattgt tctgataaaa attaaactgt tacggcttca
     3901 aaagctcttg tttttgcttt ggttccttct actaacgaaa taaatatttc atttaaacct
     3961 ggtaatattt cgttgaaaga ctctatggct tgattagtat caataaaatg ctttaataca
     4021 tcgttacttt tataaccatt attaattgaa actgtgagag agttattatt ttgtgcatct
     4081 atagtaaata cgttgcttaa agtattggta ggctcgtttt gcagtgtaat gctaaattta
     4141 tttttcttga attgttgttt tatatcttga actgtaccat ctaatatttt ctttcctaag
     4201 tttactaaaa caatatgatc gcaaatttct tctacttgct ccatgcggtg agtgctaaaa
     4261 attactgtac ttcctctttg tgctaagcca taaatttcgt ctttaattaa gttagcatta
     4321 atagggtcta atccactaaa aggttcatct aagattatta atttaggttc atgtaaaaca
     4381 gtggttacaa attgtaattt ctggctcatt cctttactta agtcttccac ttttttattc
     4441 caccaacttt ccatttctaa tttcttaaac caaaaaataa ttctctccat tgcatctttt
     4501 ttgctcattc cttttagttg agccaggtat agtgcttgtt cgccaatttt cattttcttg
     4561 tacaatcctc tttcttctgg catatagcca attttc
//
LOCUS       DAOQHP010000159         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10953_length_4583_cov_3.990283, whole genome shotgun sequence.
ACCESSION   DAOQHP010000159 DAOQHP010000000
VERSION     DAOQHP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10953_length_4583_cov_3.990283"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2854
                     /locus_tag="PLY81_06080"
     CDS             <1..2854
                     /locus_tag="PLY81_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29829.1"
                     /translation="VTLLFIKVNAQINPVGSYDSCGAALNLDVNATCITGGQTYMTTG
                     ATKSPQATLGISNDDDVWFKFTTLAGQTHASIQISNEIGGMGSCMELWDSCNASAEIS
                     DFCGSQFNVSGLTPLKTYLVRVYTNGTFSTLSSFQMCVVNTTPPPPPPNSECAFATSI
                     QVSNNNAVCNLFPLTNNYATGSTIVSAPACLSSNYKDIWIKFVPSQTGPLSFRMQDYS
                     AIAGSAFPTYYIATYSGTNCNSLSLVNCSNYSPTSGNDYSLSGTYNSGSTYYLRILCD
                     NSIAGDFNVCLRPSVSETNYPISADSTCLRSIAINSSVDNSALYTQGSTNGIRRINQL
                     ACYGYNAPNALAWYSFTVPSNGNYFVDFTDLTRLDVNAFGAGYRVLRRATCYSTGTDT
                     IITKPPNSTYDTLLCANSFDNGQMLTLTTGTQYYVTVMENSYNGGRVAYKLRVIGAST
                     CVNDDSTNAINIIQDVNCNNAINTSLRFSTLSTQPNISGLTNNGSLIQDVWYKFNAIT
                     SSVIINVTLPLYSSRIVVYNSDGTIKYDPGTNGNIITVNSLVIGARYLIRVFNTNGLP
                     IGPSADFKICVFGASPSVATSVSSCTPADATNISTNSNQWLHFQRSGGLIVSVFDGPA
                     KPGFTFLPRGTISVSYFTNNGGIRLNAGTSYLDRNFEISDGGNNFSNSPVRVRFYLEP
                     GEFNTLVASAASGGISAPYELKVYRIPGASCSNSTTAGGLYYNIDSYGFISNSTTPST
                     PTGYYVDMITPNFSGFFLQHAADNLLVPSTCNNFYYKTYNNSVQLFFSTLQENNNSYY
                     QIERSENGENFVPITSFNSNNNINGSKYSYTDYTIKPNAFYYYRIKQVDKDNSYQFIC
                     KTILVSTKKVEKTFGKIYPNPVMESFNIDINKPFAGKALVQILNANGQIVYSSTINIS
                     LSDQKLNIKTPFISRGVYTLKLLTDKGIEQMQFIKH"
     gene            3209..>4583
                     /locus_tag="PLY81_06085"
     CDS             3209..>4583
                     /locus_tag="PLY81_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29830.1"
                     /translation="MKKFFYTLIFLCIANISFANFVSPGTGVKWNLDSLVAHSAGIVT
                     FNGAEYIVNDTVFIVTNDTLSITTDAVVKYYPLTYLDVNGVLLINPPNNVKFTAINSE
                     AGFNGMRIDSSNATVLKKLTFEYAVSLKTTDCNITIDSCTFQYNNVGSSTSFGNGALS
                     LFRASPTITHSQFLNNRRAAIQGGANIANAPQIFNNIFYGNGSMNVNVPHINIGATGS
                     DTLRIINNEIIGVPAAIQTGAIGFLPIGSANTIIAGNIIKHNRYGITTNGGSNIYALI
                     KYNIIDSNNIQNNPNLGGSGIAFSGGSATSHQNAIVTGNLIRGNLWGITIQGRSKPNI
                     GNVENVDTTDDGKNVFLQNNNVNTPWTDLYNNTVDSIYAQGNYWGTNIEDTVESRIFH
                     QVDNASLGIVLYSNFILPIRLSSFSISKNNNIFYLNWKTAEELNSDYFEVERSYDGKT
                     FEFVGSVI"
BASE COUNT         1593 a          669 c          742 g         1579 t
ORIGIN      
        1 tgttacttta ctatttataa aagtaaatgc acaaataaat cctgttggca gctacgatag
       61 ctgtggggct gcattaaact tagatgtaaa tgctacttgt attacaggtg ggcaaactta
      121 tatgacaaca ggtgcaacaa aaagtccaca agcaacctta ggaattagta atgatgatga
      181 tgtttggttt aagtttacca ctttagctgg gcaaacccat gctagtattc aaatatcaaa
      241 tgaaattggt ggtatgggtt cctgtatgga attgtgggat tcatgtaatg catctgcaga
      301 gattagtgat ttttgtggaa gtcaattcaa tgtttctgga ttaacaccac ttaaaactta
      361 tttagtaagg gtttatacaa atggaacttt ctctactctt tcttcttttc aaatgtgtgt
      421 tgtaaataca acaccaccac cacctcctcc aaatagtgaa tgtgcttttg ctacatctat
      481 tcaagttagt aataataatg cagtttgcaa tttatttcca ttaactaata attatgccac
      541 gggtagtact attgttagtg cccctgcttg tttatctagt aactataaag atatttggat
      601 aaaatttgta ccttctcaaa ctggcccctt atcatttaga atgcaagatt attctgccat
      661 tgctggctct gcatttccta cttattatat agccacttat tctggtacaa attgtaacag
      721 tttatcactt gtaaactgtt ctaattattc acccacatct ggaaacgatt atagtttatc
      781 tggtacatac aattcgggtt ctacctatta ccttagaata ttatgtgata attcaattgc
      841 aggagatttt aatgtttgct taagaccatc tgttagtgaa actaattatc ctatttctgc
      901 agatagtaca tgtttacgtt caattgcaat taattcttct gttgacaata gtgcattata
      961 tacacaagga tcaacaaacg gcattagaag aattaatcaa cttgcttgct atggatacaa
     1021 tgcaccaaat gcattagcat ggtatagttt tacagtgcca tccaatggaa attattttgt
     1081 tgattttaca gatttaacta ggttagatgt aaatgctttt ggtgcaggtt atagagtgtt
     1141 aagaagagct acttgttatt caactggaac agatactata attacaaaac ctcctaatag
     1201 tacttacgat acattgttgt gtgcaaacag tttcgacaat ggtcaaatgt taacattaac
     1261 aactggaact caatattatg taaccgtaat ggaaaattcg tataatggtg gtagagtagc
     1321 ttataagctt agggtaattg gtgcctcaac ttgtgtaaat gatgacagta ccaatgctat
     1381 caatattatt caggatgtaa attgtaacaa tgcaattaat acaagtttac gatttagtac
     1441 attaagtaca caacccaata tttctggttt aactaataat ggtagcctaa ttcaagatgt
     1501 ttggtataag tttaatgcaa taacatcctc tgttataata aatgttacat taccattata
     1561 ttcttctaga atagtagtat ataattcaga tggaaccatt aaatacgatc ctggtacaaa
     1621 tggaaatatt attactgtta attcattagt aattggagca aggtatttaa taagagtttt
     1681 taatacaaat ggtttgccaa ttggtccaag tgcagatttt aaaatttgtg tttttggagc
     1741 atctccttct gttgcaacaa gtgtaagcag ttgcacacct gccgatgcaa ctaatatttc
     1801 taccaacagt aatcaatggt tacattttca aagatctggc gggttaattg tttctgtttt
     1861 tgacggacct gcaaaaccag gttttacatt cttacccaga ggtaccattt ctgtaagcta
     1921 ttttacaaat aatggtggca ttaggctaaa tgcaggaact tcatatttag atagaaattt
     1981 tgaaataagc gatggaggta ataattttag taatagccct gttagagttc gtttctatct
     2041 agaaccagga gagtttaata cattagtagc ttcagctgca agtggtggta ttagtgcacc
     2101 ttatgaatta aaagtgtata gaataccggg tgcatcttgc agtaattcaa caactgcagg
     2161 tggcttatat tacaatattg acagctatgg gtttattagt aattcaacaa caccaagcac
     2221 tccaacaggg tattatgtag atatgattac tcctaatttt tctggattct ttttacaaca
     2281 tgcagctgac aatcttttag ttccatcaac ttgcaacaat ttttattata aaacatataa
     2341 taattcggtt caacttttct tctcaacctt acaagaaaat aacaatagct attatcaaat
     2401 tgagagaagc gagaatggag aaaattttgt tcctattact tcttttaatt caaataataa
     2461 tattaatggc agtaaatact cttatacaga ttataccata aaaccaaatg ctttttatta
     2521 ttatagaatt aaacaagtgg ataaagacaa tagttaccag tttatttgca aaacaattct
     2581 agtatccaca aaaaaagtag agaaaacatt tggaaaaatt tatccaaacc ctgtaatgga
     2641 atcgtttaat atagatatta ataaaccttt tgcaggtaaa gcattagtac aaatattaaa
     2701 tgcaaatggc caaattgtgt attcctctac tatcaatatt agtttaagtg atcaaaaatt
     2761 aaatattaaa acacctttta taagtagagg cgtttatacc ttaaaactac ttacagataa
     2821 agggatagaa caaatgcaat ttattaaaca ttaattcata aatttagtac tattgtttta
     2881 ggcagatttt taaaatttct gcctattttt ttttataatt gatagttata aatcatagct
     2941 taaaaccatt ataatggaat actttaggta aaaactagga agcaattatg acaaaataaa
     3001 aattaagaaa actatacgaa aggctttaac tataatagtt catgaatata aaatgtcatt
     3061 gtttaaggaa gcaatgacat ttttgttgta gggttaaata gaaaagtaga atttatatct
     3121 tgcatatttt atttttcctt ttgtaaattt gctgtttaaa cattttaaaa actatactgt
     3181 attaacttca actttaaata gaattagcat gaaaaaattt ttttacacac taatatttct
     3241 ttgcatagca aatatctctt ttgcaaattt cgtttcacca ggtacaggtg taaaatggaa
     3301 cttagatagc cttgtagctc actctgcagg cattgttact tttaatggtg ccgagtatat
     3361 tgtaaacgat actgttttta ttgttaccaa cgatacgtta agcattacaa cagatgctgt
     3421 ggtaaaatat taccctctta catatttaga tgtaaatggt gtgttgctaa taaatcctcc
     3481 taataatgta aaatttacag ccatcaatag cgaagcaggt tttaatggaa tgcgtataga
     3541 tagtagtaat gctacagtat taaaaaaatt aacttttgaa tatgctgttt ctttaaaaac
     3601 aacagattgt aatataacta ttgatagttg cacttttcag tacaataatg ttggcagctc
     3661 tacaagtttt ggtaatggag ctttatcgtt atttagggca agcccaacta taacacattc
     3721 acaattttta aataacagaa gagcagcaat acaaggaggt gctaatattg ccaatgctcc
     3781 tcaaattttt aataatattt tttatggcaa tggatctatg aatgtaaatg tgcctcatat
     3841 aaatattgga gcaacaggaa gtgatacact aagaataatt aataacgaaa ttattggagt
     3901 tcctgctgct attcaaactg gggctattgg gtttttacct attggctctg ctaatacaat
     3961 tattgcaggt aatattataa aacacaatcg ttatggaatt accaccaacg gaggttctaa
     4021 tatttatgct cttataaaat acaatattat tgatagtaac aatatacaaa ataacccaaa
     4081 cttagggggt agtggaattg cttttagcgg aggttctgca accagtcatc aaaacgcaat
     4141 agtaacaggt aatttaatta gaggtaacct ttggggtatt acaattcaag gaaggtctaa
     4201 accaaatatt ggtaatgtag aaaatgtaga tactacggat gatggtaaaa atgttttttt
     4261 acaaaacaat aatgtaaata caccatggac tgacttatac aacaatactg ttgattctat
     4321 ttatgcacaa ggtaattatt ggggaaccaa tattgaagat acagtagagt caagaatatt
     4381 tcatcaggta gataatgcaa gtttaggaat agtactttat tcaaacttta ttttacctat
     4441 aagattatcc tctttcagta tatctaaaaa taacaatatt ttttatttaa actggaaaac
     4501 tgcagaagaa ttaaactccg actattttga ggttgaaaga agttatgatg gtaaaacatt
     4561 tgaatttgtt ggaagtgtaa ttg
//
LOCUS       DAOQHP010000160         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10988_length_4574_cov_3.969462, whole genome shotgun sequence.
ACCESSION   DAOQHP010000160 DAOQHP010000000
VERSION     DAOQHP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10988_length_4574_cov_3.969462"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLY81_06090"
     CDS             complement(<1..293)
                     /locus_tag="PLY81_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29831.1"
                     /translation="MDKTPTINNNIVKPEENFKAMSNIEKANKKGIKNGFFSFLLVLL
                     LMPIGHAIMILNEKVLHNQKYVGAFIMGFIGIGLIVWGIKKNKKANFATIMGF"
     gene            complement(407..922)
                     /locus_tag="PLY81_06095"
     CDS             complement(407..922)
                     /locus_tag="PLY81_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNF29832.1"
                     /translation="MLIKVKTIFACICILITANSFAQLNYKGFLITGNIKQDIFIGLD
                     AIAKTPSVQLKDIVITNHLNVPQLTAKNLKGFLLKEILSKVILNEPLPRKYSEFYFTF
                     IAQDSFTAVFSWNEIFNSPTGNNLFIITQKDGKSVTEMPESVLILTATDFTTGRRYIK
                     KLSAIIVSRAK"
     gene            complement(975..2240)
                     /locus_tag="PLY81_06100"
     CDS             complement(975..2240)
                     /locus_tag="PLY81_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF29833.1"
                     /translation="MKPVAIFLSLFISTIAFTQNSNKKVLFLGNSYTYVNDLPFMLSK
                     IANSTGDTVVYDSNTPGGYTLQGHYTNSTSLSKIGLGKWDYVVLQEQSQLPSFPEIDV
                     ITSVYPYAKKLDSIINEKNNCAETIFYMTWGRKNGDASNCAFWPPVCTYNGMDSLLNL
                     RYQKMADSFNAIVSPVGAVWNYIIKNYTDIELYSSDQSHPSLAGTFAAACSFYAVIFK
                     KDPTKITFNSGLNDIDANRIKTAAKTVVYDSLQKWHVGEYDAKASFTFIDSTNGKIIF
                     KNQSANANSYVWYFGDGNSSSNTNPVHTYTTNGNYTVKLLANKCSVKDSATQTIHIVV
                     SSNPPTANDAKPPSIFPIPATNSIFIQLYNNNLIGKEYNLFSSAGKLVLKGSIKTTKE
                     KIILNKLQPGLYILKIGEKNDNLYKIIKQ"
     gene            complement(2319..2885)
                     /gene="efp"
                     /locus_tag="PLY81_06105"
     CDS             complement(2319..2885)
                     /gene="efp"
                     /locus_tag="PLY81_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNF29834.1"
                     /translation="MATTSDISRGMVLKLDGNLYSVVEFGENKTARAAAKVWAKLKGV
                     DTSRTIEKTWNSGETIHPVRVEKKSFQYLYKDESGFNLMNSETFEQIILAEEMIDAPQ
                     FLKDNGEVFVLINTETDQPIGAELPEKIVVLVTYCEPGLRGDTATRATKPATIDTGAT
                     VNVPLFVEEGELIRINTKTGDYVERVKE"
     gene            3054..4034
                     /locus_tag="PLY81_06110"
     CDS             3054..4034
                     /locus_tag="PLY81_06110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNF29835.1"
                     /translation="MPQYPNRQFLDFEQPIKELYEQIDQTKKLAEKNPKIDYSNTLHQ
                     LEQSIIDKRKEITLHLTPWQRVQLSRHPDRPYTLKYIEKMTTDFIELHGDRNVKDDKA
                     MVGGFANLNGETVMFIGQQKGINTKSRQLRNFGMANPEGYRKALRLMRLAEKFNKPIV
                     TLIDTPGAYPGLEAEERGQGEAIARNIYEMIRLKVPVICVIIGEGASGGALGIGVGDK
                     MLMMENTWYTVISPESCSSILWRSWDKKEIAAEQLKLTATDMHGFGLVDEIVPEPDGG
                     AHWNYDKAAEILKQHIINSLNEYKNISANERINNRIAKYEKMGFWNEVTA"
     gene            4061..>4574
                     /locus_tag="PLY81_06115"
     CDS             4061..>4574
                     /locus_tag="PLY81_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNF29836.1"
                     /translation="MSLRSQLSGTGVAIITPFKKDYSVDYEALGKLIDFIIEGGVNFI
                     VTLGTTGETPVLDKKEKIDIINYTFDKVNNRVPVVVGIGGNNTDALIKDFEQYPIQKA
                     AAILSASPYYSKPSQEGIFQHYKALAEACPKPILIYNVPGRTGRNVSAATTIRLANEV
                     PNIGGIKEASD"
BASE COUNT         1595 a          677 c          738 g         1564 t
ORIGIN      
        1 aaaaacccca ttatggttgc aaagtttgct tttttatttt ttttaattcc ccaaactatt
       61 aaaccaattc caataaagcc cataataaaa gcccctacat atttttggtt atgtaaaact
      121 ttttcgttta aaatcattat tgcatggcca atgggcatta agagtaaaac cagaagaaaa
      181 gaaaagaagc catttttaat accttttttg ttagcctttt caatattgct cattgcttta
      241 aagttttctt cgggcttaac aatattatta ttaattgtag gtgttttatc cattttaata
      301 tttggtaaag tgtctaatat tttgttaggg tgctaaaata ttattgctaa ctaaaatgga
      361 aagtagcaat tgggaaatat tttgtagaaa taaggaaaat gagaaattac ttagctctgc
      421 taactataat ggcagataat ttttttatat atcttctgcc tgttgtaaaa tctgttgcag
      481 ttagtattaa aacagattcg ggcatttctg ttacgctttt tccatctttt tgggtaataa
      541 taaaaaggtt attacctgta ggcgagttaa aaatttcatt ccaggagaat acagcagtaa
      601 aactatcttg agcaatgaaa gtaaaataaa attcgctata ctttcttggt aatggttcgt
      661 ttaaaattac tttagataaa atttctttta ataaaaatcc ttttaaattt tttgcagtta
      721 attgtggtac atttaagtgg ttagtaatta ctatatcttt aagttgtaca gaaggggttt
      781 tggctattgc atcaagccca ataaaaatat cttgttttat atttcctgta attaaaaaac
      841 ctttataatt taattgagca aaactgttag cagtaattaa aatacatata caagcaaaaa
      901 ttgttttaac ctttattaac ataattaatt tgtagtacta aagataaaat aaaatgcctt
      961 ttgagattaa aaatttattg tttaattatc ttgtataggt tgtcattctt ttctccaatt
     1021 tttaaaatat ataaacctgg ttgtaatttg tttaatataa ttttttcttt ggtggttttg
     1081 atgcttccct ttaaaactaa tttacctgcc gatgaaaata aattatattc tttaccaatt
     1141 aaattgttgt tgtatagttg aataaaaatt gagttggtag caggtatagg aaaaatagat
     1201 ggtggtttag catcattagc agtaggtggg tttgatgaaa caactatatg aatggtttgt
     1261 gttgcagagt ctttaacgga gcatttatta gctaaaagtt ttacagtgta attaccattg
     1321 gtagtataag tatgaacagg gtttgtatta gaagagctat taccatcgcc aaaataccat
     1381 acataactat ttgcattggc agattgattt ttaaaaatta ttttaccatt agtactatct
     1441 ataaaagtaa aacttgcttt tgcatcgtat tcgccaacat gccatttttg taggctatca
     1501 taaaccacag ttttggcagc agtttttatt ctatttgcat ctatatcgtt taagccacta
     1561 ttaaatgtaa ttttagttgg gtctttctta aaaataactg cataaaaact acatgcagca
     1621 gcaaatgtac ctgcaagtga tggatgactt tggtctgatg aatacaattc aatatctgta
     1681 taatttttta taatataatt ccaaacagca ccaacaggcg aaacaatggc attaaaacta
     1741 tctgccattt tttggtatcg taaatttagt aaactatcca taccattgta agtgcaaaca
     1801 ggtggccaaa atgcacagtt agaggcatct ccattttttc ttccccatgt catgtaaaaa
     1861 attgtttcgg cacaattatt tttttcatta ataatactgt ctaatttttt tgcatagggg
     1921 tacacactag ttattacatc tatttctgga aaggaaggca attgactttg ctcttgcaac
     1981 actacataat cccacttgcc taaaccaatt tttgataaag aagtagaatt ggtataatgc
     2041 ccttgtaatg tataaccacc aggtgtatta ctatcgtaaa ccacagtatc tcctgtagag
     2101 tttgcaattt ttgaaagcat gaatggcaaa tcgtttacgt aagtataact attacctaag
     2161 aataaaactt ttttattgct attttgtgta aaagctattg tagaaatgaa aaggcttaaa
     2221 aatattgcta ccggtttcat aaattaaaaa aacgtttaaa aatacgaatg gctaccaatt
     2281 aagtagccaa tcttataatt attaaagtaa aagaataatt attcttttac tctttctaca
     2341 taatctcctg ttttagtatt tattctaatt aattcacctt cttctacaaa taaaggaacg
     2401 tttacagttg cacctgtatc aatagttgca ggttttgtag cccttgttgc agtatcacct
     2461 cttaagcctg gctcgcagta ggttactaat acaacaattt tttctggtag ttctgcacct
     2521 attggttgat ctgtttcggt attaattaaa acaaaaactt cgccattatc ttttaaaaat
     2581 tgtggggcat caatcatttc ttctgctaaa ataatttgct caaaggtttc gctattcata
     2641 aggttaaaac cactttcatc tttatacaaa tactgaaaag attttttttc aacccttaca
     2701 gggtgaatag tttctccact attccatgtt ttctcaattg tacggctggt atctactcct
     2761 ttaagttttg cccaaacttt tgctgcagca cgggctgttt tattttcgcc aaactctacc
     2821 acactataca aattaccatc taattttaaa accattccac gactaatatc tgatgttgtt
     2881 gccatatatt atttatttag agtggcaaaa gtaagggttg aacaagaaaa aaattaaact
     2941 caagttttaa ttttattact cttgccatta gaaatataga attgaaataa aaatattgat
     3001 tttataacct ataaacacgc aaagtattat ttttacaaac caattaataa agtatgccac
     3061 aataccccaa cagacaattt ctggattttg agcagcctat taaagaattg tatgaacaaa
     3121 ttgaccaaac caaaaagctt gcagagaaaa acccaaaaat tgattatagc aatacactac
     3181 atcaattaga gcaatcaatt attgataaaa gaaaagaaat aaccctgcat ttaacaccct
     3241 ggcaaagagt tcagttaagt cgtcatccag atagacctta tacattaaag tatattgaaa
     3301 aaatgactac cgattttatt gaattacatg gcgataggaa tgtaaaagac gataaagcaa
     3361 tggtaggtgg atttgcaaac ttaaatggcg aaacagtaat gtttattggt cagcaaaagg
     3421 gtattaatac caaatctaga cagttaagaa actttggtat ggcaaatccc gaaggatata
     3481 gaaaagcatt gcgtttaatg cgtttagccg aaaagtttaa taaaccaata gttactttaa
     3541 tagatacacc aggagcttat ccagggttag aagcagaaga aagaggacag ggagaagcca
     3601 ttgccagaaa tatttatgaa atgataaggc ttaaagttcc tgtaatatgt gtaattatag
     3661 gagagggagc aagtggaggt gctttaggta taggcgttgg agataaaatg ctaatgatgg
     3721 aaaatacctg gtacactgtt atttctccag aaagttgctc ctctatttta tggcgtagtt
     3781 gggataaaaa agaaattgct gcagagcaat taaaacttac tgctaccgat atgcatggct
     3841 ttggtttggt agatgaaatt gtacctgaac cagatggtgg agctcattgg aattatgata
     3901 aggcagcaga aattttaaaa caacacatta ttaattcact taacgagtat aaaaatataa
     3961 gtgcaaatga acgtattaat aacagaattg ctaagtacga aaaaatgggc ttttggaatg
     4021 aagtaacagc ttaatttaat tttttaaatc agaaatatat atgtctttac gttctcaatt
     4081 atcaggcact ggtgttgcca ttataacccc ttttaaaaaa gattatagtg tagattatga
     4141 agcacttggt aaacttattg attttataat agaaggtggt gtaaacttta tagtaacact
     4201 tggtacaacc ggcgaaacac ctgttttaga taaaaaggaa aaaatagaca ttataaacta
     4261 tacttttgat aaggtaaaca atagagtgcc ggtagttgta ggaattggtg gaaataatac
     4321 cgatgcttta attaaagatt ttgaacaata ccccattcaa aaagctgctg ctattttaag
     4381 tgcctctcca tattatagta aaccaagcca agaaggtatt tttcaacatt ataaagcatt
     4441 ggcagaagct tgccccaaac ccattttaat ttataatgta ccaggtagaa caggacgaaa
     4501 tgtaagtgct gccaccacta tacgtttagc caatgaagta cctaatattg gaggcattaa
     4561 agaagctagt gatg
//
LOCUS       DAOQHP010000161         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_10991_length_4573_cov_4.320053, whole genome shotgun sequence.
ACCESSION   DAOQHP010000161 DAOQHP010000000
VERSION     DAOQHP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10991_length_4573_cov_4.320053"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLY81_06120"
     CDS             complement(<1..702)
                     /locus_tag="PLY81_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF29837.1"
                     /translation="MGLFTKKSIDILLSEANETGENSLKRTLGGWGLIALGIGAIIGA
                     GLFVRTAAAIAQNAGPSVTIGFLVAALGCTFAGLCYAELSSSIPISGSAYTYTYATMG
                     EFLAWIIGWDLVLEYAVGAATVGIAWSEYLNNLLVEVFHLSPIPYEWCHSPFQTSVDG
                     VNGIINLPALGIVVLLSLLLIRGIKESTLVNNFIVILKVAIVILIIALGWSYINPAYH
                     TPYIPDATKYTDHHGT"
     gene            861..2132
                     /locus_tag="PLY81_06125"
     CDS             861..2132
                     /locus_tag="PLY81_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNF29838.1"
                     /translation="MSTPSTTAPKKSKLTLWIIVAMILGVLVGYLVFNNTTETFRKGF
                     SGNIKLLSTVFIRLVQMIIAPLVFTTLVVGISKLGDLKTVGRVGGKAMIWFITASLIS
                     LLIGLVLVNYFKPGQYIQLGLQDSEGLKDLMTKQTSFSLQNFVEHVIPKSFIEAMATN
                     EILQLVIFSIFFGVASASIGEYAKPVIHVLEVASHIILKIVNYVMNFAPLGVFGAIAA
                     VVASKGLVIFDFYIKYFLFFLIGIAALWIVLLTVGFIILKGRLKILLKRIGNPLLIAF
                     STTSSEAVFPKLTEELERFGCKNKIVSFVLPLGYSFNLDGSMMYMTFASIAIAQVYNV
                     PIATDLPQQLTMLLVLMLTSKGIAGVPRASLVVVLATCAMFNIPPIGVALILPIDHFC
                     DMFRTATNVIGNALATSVVSKWEGELENSNT"
     gene            2237..3172
                     /locus_tag="PLY81_06130"
     CDS             2237..3172
                     /locus_tag="PLY81_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNF29839.1"
                     /translation="MKQSLFLLILCICISSISFAQTDTITIKVQNQYEAEVEAVNIKV
                     LSTGKSFVANEKGFATINASQIDSIELTKEGYTSTVVTVNQLKNKKVVIEKKFTWKDL
                     LTPMFYIINGGLWLLLFIVFAETGLFVGFFLPGDSLLFVAGIFSNMLAAYFFDTGSDF
                     VNLLMIISLVSLAGILGNTTGYWFGRKIGPAMYHWKDNFLFKQKYLQQAHEFYEKYGG
                     GAIVFARFLPVVRTFAPIIAGIVQMDKKKFGLFNIIGSFAWAGSMIAAGHYLDKLFID
                     KFDIDLKKHLEIIIIIIVIVSNTPVIIKLLGKKKH"
     gene            3303..4550
                     /locus_tag="PLY81_06135"
     CDS             3303..4550
                     /locus_tag="PLY81_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF29840.1"
                     /translation="MNPQKNYSVLSLPVIVAALGYFVDIYDLLLFTIVREPSLQGIGI
                     NLADKAAVLNASTKIINWQMLGLLIGGIVWGTIGDKKGRLSVLFGSIAMYSVANFLTG
                     YVQTTGQYSFARFVAGIGLAGELGAGITLVSELLPKNKRGIGTSLVAGIGLFGAVFAY
                     YTFYITNQDWRLCYKIGGGLGFILLLLRVSVAESGMFKEVKNKAIQRGNFFMFFTNAK
                     RFKKYAFAILIGLPTWYVIGVLVNYSNRFATEFYGTNAIISGKAIMYAYIAIAFGDIL
                     IGFVSQYFKSRKKALLLFYILCIIGMILFYSPLNNSDFNMYAICAFLGFSTGFWAIFV
                     TIGAEQFGTNLRATAATTIPNMVRGALPLINLLFLDVFQKSWNLSLIVSGIITAVIVM
                     SIALIAFYYTEETFHKDLNYIEE"
BASE COUNT         1489 a          629 c          792 g         1663 t
ORIGIN      
        1 agtaccatga tgatctgtat attttgtggc atcaggaatg taaggtgtgt gataagcagg
       61 gttaatataa ctccaaccta aagcaattat taaaataaca atagcaactt ttaaaataac
      121 tataaagttg tttactagtg tagattcttt aatacctcta attaataata aactaagtaa
      181 aacaacaata cctaaagcag gtaaattaat aatgccatta accccatcta cagaggtttg
      241 aaatggtgaa tgacaccatt cataaggtat agggcttaaa tgaaaaactt ctactaataa
      301 attgttcaaa tattcactcc atgcaatacc aacagtagca gcaccaactg catactcaag
      361 taccaaatcc catccaatta tccatgctaa aaattcaccc atggtagcat aagtataggt
      421 gtatgcacta ccagatatag gaatagagga agaaagctca gcataacaca aacctgcaaa
      481 agtacatcct aaggccgcta ctaaaaaacc tatagttact gaaggcccag cattttgagc
      541 aatggctgca gctgtacgaa caaataaacc agctcctata attgcaccaa ttcctaaagc
      601 aattaaaccc catcctccta aggttctttt aagtgagttt tcgccggttt catttgcttc
      661 agatagcaaa atatcaatag acttctttgt aaataatccc ataaaattgt tttggttaat
      721 agtttcagtt tgattgccta aaaataggga taatcttaaa taattattat aaaactttta
      781 ttagtggtta ttatttgatt agtgtagata ttgagctaaa tactatatat tgccttttta
      841 aattagaaaa taaaacatac atgagtactc caagtactac tgcccctaaa aaatctaaac
      901 taacactttg gataattgtt gccatgattc ttggcgtttt ggtaggttat ttagtgttta
      961 ataatactac cgaaactttt agaaaaggat tttcaggtaa cattaaattg ttaagcactg
     1021 tgtttatacg cttggtacaa atgattattg ctccattagt ttttaccacc ttagtagttg
     1081 gtatttctaa acttggcgac ttaaagactg ttggtagagt aggaggtaaa gctatgatat
     1141 ggtttattac tgcttcttta atttctttat taataggctt agtacttgta aattatttta
     1201 aacctggcca atacattcaa ttaggattac aagacagcga agggttgaaa gatttaatga
     1261 ctaagcaaac atctttttca cttcaaaatt ttgttgagca tgtaatacct aaaagtttta
     1321 tagaggcaat ggctactaat gaaattttac agttggtaat tttttctatc ttttttggtg
     1381 tagcatcagc atcaattggt gagtatgcaa aacctgtaat tcatgttctt gaagttgctt
     1441 ctcatataat tcttaaaata gttaactatg taatgaattt tgctccatta ggtgtttttg
     1501 gagcaatagc tgctgtagtt gcttctaaag gattagtaat ttttgatttt tatattaaat
     1561 attttttatt ctttttaatt ggaattgcag cactctggat agtattgtta actgttgggt
     1621 ttattatttt aaaaggcagg ttaaaaattt tacttaaaag aattggtaac ccattattaa
     1681 ttgcttttag tacaacaagt agcgaagctg tatttccaaa acttacagaa gagttagaaa
     1741 gatttggttg taaaaataaa attgtttcgt ttgtacttcc attgggttat tcatttaact
     1801 tagatggtag tatgatgtac atgacttttg caagtattgc tattgctcag gtttataatg
     1861 taccaatagc aacagatctt ccacaacaac ttactatgtt acttgtttta atgcttacaa
     1921 gcaaagggat tgcaggtgta ccaagggcaa gtttagtagt ggttttggct acttgtgcta
     1981 tgtttaatat tccaccaatt ggtgtagctt taatattacc aatagatcat ttttgcgata
     2041 tgtttagaac agcaaccaat gtaattggta atgcccttgc aacaagcgtt gtaagtaaat
     2101 gggaaggtga attggaaaat agtaacacct gatttactat ttttttaaca accctactac
     2161 atttttttat tatatcattg ccaaataata attaccaaca ctagtcctta ttaatacaga
     2221 actgcactta atttttatga aacaatctct atttttattg atactatgca tttgtatttc
     2281 tagcatttct tttgcacaaa cagatacaat tacaattaaa gttcaaaacc aatacgaggc
     2341 agaagtagaa gctgtaaata taaaagtact aagtacagga aaaagctttg tagctaatga
     2401 aaaaggtttt gcaaccatca atgcatctca aatagattca atagaattaa ctaaagaagg
     2461 ttataccagt acagttgtta cggttaatca acttaaaaac aaaaaggttg taattgaaaa
     2521 aaaattcact tggaaagact tgctaacccc aatgttttat attattaatg gagggttatg
     2581 gttactctta tttattgtat ttgccgaaac agggttattt gttggatttt ttctaccagg
     2641 ggatagttta ttatttgttg caggaatatt cagtaatatg ttagctgctt atttttttga
     2701 tactggtagc gattttgtaa atcttttaat gattatttcg ctggtttcat tagctggtat
     2761 tttaggtaat actacaggtt attggtttgg aagaaaaatt gggcctgcaa tgtatcattg
     2821 gaaagataac ttcttattta aacaaaaata tttgcagcaa gcacacgagt tttacgaaaa
     2881 atatggtggt ggggcaattg tatttgctag gtttttacct gttgtaagaa cttttgcacc
     2941 aattattgcc ggcatagtac aaatggataa aaagaaattt ggattgttta atattattgg
     3001 ctcatttgcc tgggcaggta gtatgattgc tgctggccat tacttagata aattgtttat
     3061 tgataagttt gatattgatt tgaaaaaaca tttggaaatc atcattatca ttattgttat
     3121 tgtttctaat actcctgtaa ttataaagct tttaggaaag aaaaaacatt aattatcttc
     3181 tctgtttttt atttgtttga tgtttgagtt gttgcaaact gtaattttat ttcagttaat
     3241 gcattttttt taattcaaat tttgatactt ttcaaaatag tcatcaaaca actaatcact
     3301 acatgaatcc acaaaaaaac tatagtgttc tttcacttcc tgtaattgtt gctgcattag
     3361 gttactttgt agatatttac gacttattac tttttaccat tgtaagagaa cccagtttac
     3421 aaggtattgg tataaacctt gcagataaag cagcagtact caatgcaagt actaaaatta
     3481 ttaactggca aatgttgggc ttacttattg gaggtattgt ttggggaaca ataggagata
     3541 aaaaaggaag gctaagtgta ttatttggat ccattgcaat gtattctgtt gctaattttt
     3601 taactggtta tgttcaaaca acagggcagt atagttttgc aaggtttgtt gcaggcattg
     3661 gccttgctgg cgaattaggt gcaggaatta ctttagtaag tgaattgctt cctaaaaaca
     3721 aaagaggcat tggtacatct ttagttgcag gaattggttt gtttggtgct gtatttgctt
     3781 attatacttt ttatataact aatcaagatt ggagattgtg ttacaaaatt ggcggaggat
     3841 taggttttat tttattgctt ttaagagttt ctgttgcaga gagtggaatg tttaaagaag
     3901 taaaaaataa agccattcaa cgtggtaatt tttttatgtt ttttaccaat gccaaaaggt
     3961 ttaaaaaata tgcctttgct attttaattg gtttgcctac ctggtatgta attggcgttt
     4021 tggtaaatta tagtaaccgt tttgcaactg aattttatgg aaccaatgct atcattagtg
     4081 gtaaggcaat tatgtatgct tatattgcta ttgcctttgg cgatatttta attggctttg
     4141 ttagtcagta ttttaaaagt agaaaaaagg cattactact tttttacata ctttgtatta
     4201 taggaatgat attgttttat agcccactaa acaattctga ttttaatatg tatgcaatat
     4261 gtgcctttct tgggtttagt actggctttt gggcaatttt tgtaacaata ggtgccgaac
     4321 agtttggtac taatttaaga gctacagcag ccaccacaat tcctaatatg gttcgtggtg
     4381 cattaccttt aattaattta cttttcttag atgtttttca aaaaagctgg aatttgagtt
     4441 taattgtgag tggcattatt actgctgtaa ttgtaatgag tattgcactc atagcatttt
     4501 actatacgga agaaactttt cataaagatt tgaattatat tgaagagtaa aaaagaagag
     4561 tgcttaaaat ggt
//
LOCUS       DAOQHP010000162         4552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11067_length_4552_cov_4.344897, whole genome shotgun sequence.
ACCESSION   DAOQHP010000162 DAOQHP010000000
VERSION     DAOQHP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11067_length_4552_cov_4.344897"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLY81_06140"
     CDS             <1..1115
                     /locus_tag="PLY81_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNF29841.1"
                     /translation="IDITIVRENIEDTYGGVEHMLTHDVALSRRFITRPGSMQVIRYA
                     FEMAKKKGAKRITCGHKANIMKITDGLFLDVFYEVAKEYPELNADDVIVDDLCMKLVT
                     KPNLFDVVVLTNLQGDIVSDLCAGLVGGLGFAPSANIGDHISIFEAVHGTAPDIAGKN
                     IANPTSLLLSGVGMLQHIGLMESASLIENALLYTLESGVHTGDFGDKTTPALNTTEFA
                     DTIIANFGKQPKHNPKAYLNDVPFSCSVLHLDKNPMLETTENTGEKIVGVDMFIESNV
                     QPEELAKKCLRHAGVKFKLVNISNRGTQVWPTGSRYTNLVNQYNARFESIDDTALNQQ
                     DIIGLYVNLSGDFKICSLELLNSWGDKKSYSLVQGQ"
     gene            1127..1585
                     /locus_tag="PLY81_06145"
     CDS             1127..1585
                     /locus_tag="PLY81_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF29842.1"
                     /translation="MINIRRAEKADCSRLLELIKELAHFEKAPEEVTVSFEHFVESGF
                     GTNPVWKGFVASTTENNIEKIVGFALYYIRYSTWKGQRMYLEDIIVSEEMRGKGIGGK
                     LFEQLIEEAKNNKLNGIVWQVLNWNEPAIQFYKKYQAKFDDEWINVSINF"
     gene            complement(1594..2514)
                     /locus_tag="PLY81_06150"
     CDS             complement(1594..2514)
                     /locus_tag="PLY81_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF29843.1"
                     /translation="MFKEYFTYSKRERNAIIILLLIIIASIIIPFFYQPKFNTNKINI
                     ALADSINALQKRSTTAKKYSKNWSNQQSDFNDEKVTEIILFYFDPNTLDMDGWKKLGL
                     RDKTINTILNYISKGGKFRTPEDIRKIWGLKKEEADRLIPYIRIKPDETYKTSANRTK
                     QVINTNKNEKKSLLDINTVTAWQLKQIPNIGNSLPYKIVTYREKLGGYLFIHQLKEVY
                     EMTDTLYHNILPYIQLIPIKIKKINLNKATEYDLSMHPYIDKNIAKAIVIQRTKYGTY
                     NSIQELKTKIVFLKEEVFLKFEPYITVQDE"
     gene            complement(2555..3331)
                     /locus_tag="PLY81_06155"
     CDS             complement(2555..3331)
                     /locus_tag="PLY81_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF29844.1"
                     /translation="MITPLKITFLGTGTSSGVPMIGCNCMVCTSRNTKDKRLRSSILV
                     QSATTTIVVDTTPDFRYQMLCTNTKHVDAILFTHPHKDHIAGLDDVRAFNFLTNQPMQ
                     LYANSLTAEALRRDFYYAFSDKKYPGVPNLNIHIIDTNSFFIGDIPIQPILVWHLNMP
                     VFGYKFGNFTYITDANRIDEKEQKLISNSTILVLNALRKEKHISHFTLDEATQLAQVL
                     QAQKTYFTHISHQLGLYNEINNSLPKNIELAYDGLVVEVS"
     gene            complement(3505..4512)
                     /locus_tag="PLY81_06160"
     CDS             complement(3505..4512)
                     /locus_tag="PLY81_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNF29845.1"
                     /translation="MATKKPALKKTVKPVKKAAPAKKAAPVKKAALAKKAAPVKKAAP
                     AKKVAPVKKAAPAKKVAPAKKAATKPAAKKVDKSVKKDKKIPKLEKPLKKEVKPEAKK
                     VEKVIKPEKIKKEKAPKPAKEPKVVAPKKILKAGMPLKKTPVKPVKASGIEKSTTVIP
                     MKQKPMIKKEEPVKDVKIPKTSVKTSVAYKPGYTPLENRVEKSAPGAPLVKYSDSDLN
                     EFREIIFKKLEAAKKELAYFQGVITRKDEGGEGEEGRYMTMEDGSLSMEREQLSQMAS
                     RTINYIDNLKKALMRIENKTYGICRVTGKLIDKARLRAVPHATLSLEAKLGLVKPSSA
                     E"
BASE COUNT         1410 a          667 c          736 g         1739 t
ORIGIN      
        1 ctattgatat tactattgta agagaaaata ttgaagatac ttatggtggt gttgaacaca
       61 tgcttactca tgatgttgct ttaagcagaa gatttattac cagaccaggt agcatgcaag
      121 ttatcagata tgcttttgaa atggcaaaga aaaaaggagc caaaagaatt acctgtgggc
      181 ataaagcaaa cattatgaaa attaccgatg gcttattttt agatgtgttt tatgaagttg
      241 ctaaagaata tcccgaatta aatgcagatg atgtaattgt tgatgattta tgtatgaagt
      301 tagtaactaa acccaattta tttgatgtgg ttgttttaac taatttacaa ggagatattg
      361 taagcgattt atgtgcaggc ttagttggtg ggttaggctt tgcaccaagt gccaatattg
      421 gagaccatat ttctattttt gaagctgtac atggtactgc accagatatt gctggaaaaa
      481 atattgctaa tccaacttct ttattgttaa gtggtgttgg catgttgcag catataggat
      541 taatggaaag tgcaagttta attgaaaatg ctttattata tactttagag agtggtgtac
      601 atacaggaga ttttggagat aaaacaaccc ctgcattaaa tactacagag tttgcagata
      661 ctattattgc caattttggt aaacaaccaa aacataatcc taaagcttat ttaaatgatg
      721 tacccttttc ctgctctgtt ttacatttag ataaaaaccc aatgcttgaa acaacagaaa
      781 acactggtga aaaaattgtt ggagtagata tgtttattga aagcaatgtg caaccagaag
      841 aattagcaaa aaaatgttta aggcatgcag gtgtaaaatt taagttagtt aatattagta
      901 atagaggcac acaagtttgg ccaactggta gccgttatac caatttagta aatcaataca
      961 atgctcgttt tgaaagtata gacgatactg ccttaaacca gcaagatata attggtttat
     1021 atgttaacct aagtggcgat tttaaaattt gtagtttaga attattaaat agctggggcg
     1081 ataaaaaaag ttatagcctt gtacaaggtc agtaataata taaaggatga taaacattag
     1141 aagagccgaa aaagcagact gtagccgttt attagaattg ataaaggaac ttgcacattt
     1201 tgaaaaagct cccgaagaag taacagtatc ttttgagcat tttgtagaaa gtggttttgg
     1261 cacaaaccct gtatggaagg gctttgtagc ttctacaaca gaaaataata tagaaaaaat
     1321 tgtaggcttt gcattatact atattagata tagtacatgg aaggggcaaa gaatgtattt
     1381 agaagatatt attgtttcgg aggagatgag aggaaaagga attggtggta aattatttga
     1441 acaattaatt gaagaggcaa aaaataacaa attaaatggt attgtttggc aagtgctgaa
     1501 ctggaatgaa ccagctattc aattttataa aaaataccaa gcaaaatttg atgatgaatg
     1561 gataaatgtt agtataaatt tttagactat actctattca tcttgtacag ttatataagg
     1621 ttcaaacttt aaaaaaactt cttcttttaa aaaaacgatt ttggttttta attcttgtat
     1681 ggaattataa gtcccatatt tcgttctttg tattactata gctttagcaa tatttttatc
     1741 aatatagggg tgcatactta aatcatattc tgttgctttg tttagattaa tttttttaat
     1801 ttttattggt atcaattgta tataaggtaa aatattgtgg tataatgtat ccgtcatttc
     1861 atacacttct tttagctgat gaataaataa ataacctccc aacttttctc tataagtaac
     1921 tatcttataa ggtaatgagt tgcctatatt gggtatttgt ttaagctgcc aagctgttac
     1981 agtattaata tctagtaaag attttttttc atttttgttt gtattaataa cttgctttgt
     2041 tctgttggca cttgttttat aagtttcgtc aggctttatt cttatatagg gtattaatct
     2101 atctgcctct tcttttttta agccccatat ttttctaatg tcttctggtg ttctaaattt
     2161 tcctccttta gatatatagt ttaaaatagt gtttattgtt ttatctctta gtcctaattt
     2221 tttccaacca tccatatcta aggtattggg gtcaaaataa aataaaataa tttcagtaac
     2281 cttttcatca ttaaaatctg attgttgatt actccaattt ttagaatatt tttttgcagt
     2341 agtacttctt ttttgtaagg cattaataga atctgccaaa gcaatattta ttttattggt
     2401 attaaatttt ggttggtaga aaaagggaat aatgatgcta gcaataataa taagtagaag
     2461 aattattatt gcatttcttt ctctttttga atatgtaaaa tactctttaa acatctttac
     2521 atttcaatta tttatctgta agtactaact tttcttaact tacttctaca actaatccat
     2581 cataagctaa ttcaatattt ttgggtaagc tgttatttat ttcattatat aaaccaagtt
     2641 gatggctgat atgtgtaaag taagtttttt gggcctgcag tacttgggct aattgggtag
     2701 cttcgtctaa cgtaaaatga gaaatatgct tttcttttct taatgcattt aaaaccaaaa
     2761 ttgttgaatt actaattaat ttttgttctt tttcatcaat tctatttgca tcagttatat
     2821 aagtaaagtt tccaaatttg tacccaaata caggcatatt taaatgccaa accaaaatag
     2881 gttgtattgg aatatcgcca ataaaaaagc tatttgtgtc aataatatgg atattcaaat
     2941 ttggcacgcc aggatatttt ttatctgaaa atgcataata aaaatctctt ctcaaagctt
     3001 cggcagttag agagttggca tataattgca taggctgatt agttaagaaa ttaaatgctc
     3061 ttacatcatc taatcctgca atatgatctt tatgggggtg agtaaataaa atggcatcca
     3121 catgtttagt gttagtacac aacatttgat atctaaaatc tggtgtagta tctaccacaa
     3181 tagttgttgt agcagattga accaaaatgc tacttcttag ccttttatct ttagtatttc
     3241 ttgaggtaca aaccatacaa ttgcagccaa tcattggtac accgctactt gtacctgttc
     3301 ctaaaaaagt aatttttaaa ggcgtaatca ttacattcaa atttacatta aaatttatat
     3361 aatattattc tataaattat ttaatagtta aagattcctt caatatttat aaattaattt
     3421 tttattcatt tcttagttca ccaatttact gtaaataaaa aaagcccctt tcggggctta
     3481 aagctatatt tattactaat attgttattc agcagatgat ggttttacca aaccaagttt
     3541 agcctctaaa cttaaagttg catgtggcac agcacgtaaa cgggctttat caattaattt
     3601 accagtaaca cggcaaatgc cataagtttt attttcaatt cgcattaagg ctttcttcaa
     3661 gttatctata tagtttatgg ttctgcttgc catttggcta agttgctctc tttccatact
     3721 caaactacca tcttccatag tcatatatct tccttcttct ccttctccac cttcatcttt
     3781 acgagtaatt acaccttgaa aataagccaa ttctttctta gcggcttcta attttttaaa
     3841 tattatttct ctaaactcat tcaagtcaga atcgctatat ttcactaatg gtgcacctgg
     3901 tgcagatttt tccactctgt tttctaaagg tgtgtaccca ggtttataag caacacttgt
     3961 ttttacacta gttttaggaa tttttacatc ttttacaggc tcttcttttt taatcattgg
     4021 cttttgcttc attggaataa ctgtagtaga tttttcaatt ccactagctt tcactggctt
     4081 aacaggcgtt ttctttaaag gcatacctgc ttttaaaatc ttttttggtg caacaacctt
     4141 aggttctttg gctggttttg gtgccttctc ttttttaatc ttttctggct ttattacttt
     4201 ttctactttt ttagcctccg gctttacttc cttttttaaa ggtttctcta atttaggtat
     4261 cttcttatct ttttttacag atttgtcaac tttttttgca gccggctttg ttgcagcctt
     4321 tttggctggt gcaacttttt ttgctggagc agccttttta actggtgcaa ctttttttgc
     4381 tggagcagcc tttttaacag gtgcagcttt ttttgctagt gcagcctttt taactggtgc
     4441 agcttttttt gctggtgcag cctttttaac tggtttaaca gtttttttta gagcaggttt
     4501 tttagtagcc atagatttac gcttttttag ttaccgctat ttttagcatt at
//
LOCUS       DAOQHP010000163         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11243_length_4510_cov_3.319416, whole genome shotgun sequence.
ACCESSION   DAOQHP010000163 DAOQHP010000000
VERSION     DAOQHP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11243_length_4510_cov_3.319416"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..277
                     /gene="rpmH"
                     /locus_tag="PLY81_06165"
     CDS             122..277
                     /gene="rpmH"
                     /locus_tag="PLY81_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNF29846.1"
                     /translation="MKQTFQPHKRRRKTVHGFRKRMQTANGRKVLASRRAKGRKKLTV
                     SDERGLK"
     gene            347..739
                     /gene="rnpA"
                     /locus_tag="PLY81_06170"
     CDS             347..739
                     /gene="rnpA"
                     /locus_tag="PLY81_06170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNF29847.1"
                     /translation="MLKKYSYNKKEKLKSKKLLDEVFKKGKTFSIYPVKVFYLVTNDE
                     LESNNHLKTGVGVSKRYFKQAKKRNYIKRVLRECYRQNKLPLVDHLKNNKKHLALFFL
                     YTDKEILPFKEIEKRIKNILDKLQQQLN"
     gene            736..966
                     /gene="yidD"
                     /locus_tag="PLY81_06175"
     CDS             736..966
                     /gene="yidD"
                     /locus_tag="PLY81_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNF29848.1"
                     /translation="MKYLLIILSYPFLLLIKLYQYIISPMLGPKCRFTPTCSQYAAEA
                     LKKYGIFKGSWLALKRISKCHPAGSSGYDPVP"
     gene            978..2069
                     /locus_tag="PLY81_06180"
     CDS             978..2069
                     /locus_tag="PLY81_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNF29849.1"
                     /translation="MQEFLDKIILNNNVRSYATVFAIIIIAFIFKRYLTKYIAGWLYK
                     LVSNKEIKVKKESFLNKVIKPLDAFLILLVSIIAVDKLNFPEILDFKILKISFKTIIE
                     SVSNAAMVIVFIWLCRRMIDFIAIILEEKANATEDPTDNQLIVFFKDFFKVILVIIGI
                     LLILRFSFHKNIGNLLTGLSIVGAAIALSLRESLENLIASFIIFFNKPFVTGDVVKVH
                     NFTGTVEKVGLRSTRIRTDVKTFVTVPNKQMVDSILDNITLRTQRKIDIRLEISLSTS
                     ISQLKELTPLLQNIVTPNSVIENSLVYFLETGKNAHVFVVEVYAAMFQNINEFNVLRE
                     EINLKIIELLENQQIEFAATIADIKLHNK"
     gene            2311..3477
                     /locus_tag="PLY81_06185"
     CDS             2311..3477
                     /locus_tag="PLY81_06185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNF29850.1"
                     /translation="MRKVLLAFLLLNIFFSIKAQNKISYYRSKGKLPMMSYSKGSDRL
                     GGAKMNIIDTNILLKIIDSTHEFFTVQLSKNHTSYLEKEFAIPDTLVKPKNYYLCNSI
                     NIKSIEDSFDLVSINMEEKVPYKIWMENNPNKIMLQLYGVQSNINWINQRINTKSVKN
                     VTINQTEDDVITVTIELSNKQHWGFSTSYIHNTLNIKVKHAPKPEIKNLIIAIDAGHG
                     GNNKGTIGSKTKIEEKDYTLIYAKELEKLLTKKGAKVIMTRTTDTSFGNKDRILFLQQ
                     NSPHLLISLHFNASVKPEINGVSTYYKHIGFKPLAQSILQKMLELDLNEFGNIGQFNF
                     SLNAITDFPNCLVEIAFLSNELDEEKVLRPKFPLFVAQKIYAGLKDFLKQSADD"
     gene            3534..>4510
                     /locus_tag="PLY81_06190"
     CDS             3534..>4510
                     /locus_tag="PLY81_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF29851.1"
                     /translation="MRIVPFIISAAITTGLIITLNTQLKIGTSKTPKLGYFLSPQYGF
                     WQNAEPTDIAFNDDIKMPGVTNQVDVYFDERLVPHVYAENDADAYFIQGYLHAKFRLW
                     QMEFQTHIAAGRLSEIVGEDKIATDKYFRRLGMVYGAENTLKAIDKDAETKAACDAYT
                     AGVNTYINSLKPEQIPFEYKLLDYKPEAWTNLKTAIFLKFMSWDLAGKTEDLLFTNAK
                     TYFGYDDLQKLYPLIQDTLDPIIPKGTVYEKASITVTKPANSDSLYLERKDTINNPPT
                     VKPNKNNGSNNWAVAGIKTKSGKPILCNDPHLGLNLPSLWFEMQITTPK"
BASE COUNT         1790 a          616 c          640 g         1464 t
ORIGIN      
        1 ttatcaaact tttgctaaat tatttattac ccctatcttt gcacaccttt cacaaaatgg
       61 tggtatggtg accactattg aaatgaaaca aaattgttag gaaaagaaaa ctttaaaagt
      121 aatgaaacaa acattccaac cacataaacg tcgtagaaaa acagttcatg gttttcgtaa
      181 acgtatgcaa acagctaatg gtcgtaaagt attggcaagc cgccgtgcaa aaggacgtaa
      241 aaaattaact gtttctgacg aacgtggatt aaaataataa atagaaacaa ataaattata
      301 tttaagttct gcctttacag cagaactttt tagttttata gtatctatgt taaaaaaata
      361 ttcttataat aaaaaagaaa aactgaagag taaaaaactt ttagatgagg tgtttaaaaa
      421 aggtaaaaca ttttctattt acccagttaa agttttttat ttagtaacaa atgatgagtt
      481 ggaaagtaat aatcatttaa aaacaggtgt tggtgtaagc aaaagatatt ttaaacaggc
      541 aaaaaaaaga aactacatta aaagagtttt aagagaatgt tacaggcaaa ataaattgcc
      601 tttagtagat catcttaaaa ataataaaaa acatttagct ttattttttt tatataccga
      661 taaagaaatt ttacctttta aggagataga gaaaagaatt aaaaacatat tagataaatt
      721 acagcaacaa ttaaattgaa atatttacta attatattaa gttatccatt tttattgcta
      781 ataaagcttt atcagtatat catttctcca atgttaggtc ctaaatgtag gtttactcct
      841 acttgctcac aatatgctgc agaagcatta aagaaatatg gtatttttaa aggtagttgg
      901 ttagctttaa aaagaattag taaatgccat cctgcaggaa gtagtggcta cgaccctgta
      961 ccttaataaa atatagtatg caagaatttt tagataaaat aatattaaac aacaatgtaa
     1021 gaagttatgc aactgttttt gctataataa taattgcttt tatttttaaa agatatctta
     1081 caaaatacat tgctggttgg ctatacaaat tggtttctaa caaagaaatt aaggttaaaa
     1141 aagaatcctt cttaaataaa gtaattaaac cattagatgc ttttttaatt ttattagttt
     1201 ccataattgc tgtagataaa ttaaactttc ctgaaatttt agattttaaa atattaaaaa
     1261 tttcttttaa aacaataata gaaagtgtta gcaatgctgc aatggtaatt gtgtttatat
     1321 ggctttgtag aagaatgata gattttattg ctattatttt agaagaaaaa gccaatgcaa
     1381 ccgaggaccc aacagataat cagctaatag tattttttaa agactttttt aaagtaatat
     1441 tagttattat aggcatttta cttatactac gtttttcatt tcataaaaat ataggtaacc
     1501 tacttacagg gttaagtatt gtgggtgctg caatagcact atcgttaaga gaaagtttag
     1561 aaaatttaat agcctcattt atcatatttt ttaataagcc ttttgtaaca ggcgatgtag
     1621 taaaagtgca taattttact ggcactgttg aaaaagttgg gttaagaagt acaagaataa
     1681 gaaccgatgt taaaactttt gttactgtac caaacaaaca aatggtagat agcattttag
     1741 acaatataac tttaagaacc cagcgtaaaa tagatattcg tttagaaatt agcttatcta
     1801 ccagcatatc tcagctaaaa gaattaaccc cattattgca aaatatagtt acacccaaca
     1861 gtgttataga aaacagttta gtttactttt tagaaactgg aaaaaatgct catgtttttg
     1921 ttgtagaagt ttatgctgca atgtttcaaa acattaatga atttaatgta ttgagagaag
     1981 aaataaattt aaagataatt gaacttttag aaaaccagca aattgagttt gctgcaacca
     2041 ttgcagatat taaactacat aataaataaa cgttttaact aaactactgt aaaataattt
     2101 taaagcaatt tttaactctg ctgctacatt gttgtatcta cacacaaaat tttatctata
     2161 atacaaacat taaaacttgt ttgctaaaaa aatattctga ttttatttct cttaaaatac
     2221 ttttcgtacc ttgtaaacat cttggtttaa cttgcaatat tttaaatatt accaaataag
     2281 ccaataataa aaaaacattt caacaatttc atgagaaaag tactattagc atttctatta
     2341 ttaaacattt ttttttccat aaaagcccaa aataaaatta gttactatcg ttctaaaggc
     2401 aaattaccta tgatgtctta tagcaaaggc tctgatagat taggtggtgc taaaatgaat
     2461 ataattgaca caaatatttt attaaaaatt atagatagca cacacgaatt ttttactgtt
     2521 caattatcaa aaaatcatac ttcctattta gaaaaagaat ttgcaatacc agatacttta
     2581 gtgaagccaa aaaattacta tttatgcaat agtattaata taaaatctat tgaagatagt
     2641 tttgatttgg tatccattaa tatggaagaa aaagttcctt ataaaatttg gatggagaat
     2701 aatccaaata aaataatgct tcagctctat ggtgtacaaa gcaatatcaa ctggataaat
     2761 caacgtataa atacaaaatc tgtaaaaaat gtaaccatta accaaacaga agatgatgta
     2821 ataacagtta ctattgaact atctaataaa caacattggg gttttagcac tagttacata
     2881 cataatactt taaacataaa agttaaacat gcacccaaac cagaaataaa aaatttaata
     2941 attgcaatag atgccggcca tggtggaaac aataaaggaa ctattggaag caaaactaaa
     3001 attgaagaaa aagattacac tttaatatat gccaaagaat tagaaaaatt acttaccaaa
     3061 aaaggtgcaa aagttattat gacaagaact acagatacca gttttggtaa taaagacaga
     3121 atattgtttt tgcaacaaaa tagcccacat ttgcttatta gtttgcactt caacgcatct
     3181 gtaaagcctg agattaatgg agtaagcacc tattataaac atattggttt taaaccatta
     3241 gcacaatcta ttttacaaaa aatgcttgaa ttagacctaa acgagtttgg caatattggc
     3301 cagtttaatt tttctttaaa tgccatcaca gactttccaa actgcctggt agaaatagca
     3361 tttctatcta acgaattaga cgaagaaaaa gttttacgac ctaaattccc attatttgtg
     3421 gcacaaaaaa tttatgcagg attaaaagat tttttaaaac aaagtgccga tgattaaaat
     3481 tcattgaaga gcattaaaat aaattaacta ttttcagact ttaaataaat attatgcgta
     3541 ttgttccttt tataatttca gcagcaatca ctacaggctt aattattact ttaaatacac
     3601 aattaaaaat tggtactagt aaaacaccca aacttggtta ttttctttcg ccccaatatg
     3661 gattttggca aaatgccgaa cctacagaca ttgcatttaa cgatgatatt aaaatgccag
     3721 gtgttaccaa tcaggttgat gtttattttg atgaaagatt agtgcctcat gtatatgcag
     3781 aaaacgatgc agatgcctat tttatacaag gatatttaca tgccaaattt aggttatggc
     3841 aaatggaatt tcaaactcat attgcagcag gtaggttaag tgaaatagtt ggagaagaca
     3901 aaattgcaac cgacaaatat tttagaagat taggtatggt atatggtgct gaaaatactt
     3961 taaaagcaat tgataaagat gcagaaacaa aagcagcttg tgatgcttat acagcaggtg
     4021 ttaatactta tataaactct ttaaagcccg agcaaattcc ttttgaatat aaattattag
     4081 actataagcc agaagcttgg actaatttaa aaacagccat cttcttaaaa tttatgagtt
     4141 gggatttagc aggaaaaact gaagacttat tatttacaaa tgctaaaact tattttggat
     4201 atgatgattt acaaaaactc tatccattaa ttcaagatac attagacccc atcattccta
     4261 aaggcacagt gtatgaaaaa gcatctatta ctgtaactaa accagctaat agcgatagct
     4321 tatatttaga aagaaaagat actattaata accccccaac agtaaaacct aataaaaata
     4381 atggtagcaa taattgggca gtagctggca ttaaaacaaa gagtggaaaa ccaattttat
     4441 gtaatgatcc acatcttggc ttaaatctcc cctcactttg gtttgaaatg caaattacta
     4501 ctccaaaata
//
LOCUS       DAOQHP010000164         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11275_length_4504_cov_3.412003, whole genome shotgun sequence.
ACCESSION   DAOQHP010000164 DAOQHP010000000
VERSION     DAOQHP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11275_length_4504_cov_3.412003"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLY81_06195"
     CDS             <1..861
                     /locus_tag="PLY81_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNF29852.1"
                     /translation="IIDSIQTLQSNLIDAGAGSVSQIKECAAEFQRFAKETNTPVFLI
                     GHITKDGNIAGPKILEHMVDTVLQFEGDRHYAYRILRTIKNRFGSTAELGIYEMTGEG
                     MRAVNNPSEILISQRDDQLSGIAIAATIEGMRPLLIEVQALVTQSVYGTPQRTVTGFD
                     LRRLQLLLAVLEKRGGFLFGTKDVFVNIAGGLKIEDPSIDLAVICALLSSYDDTPISH
                     KICFAGEVGLNGEIRAVNRIEQRIAEAEKLGFEKIFVSKFNKKSVNLKLFNIDIIMVS
                     QLHEVYQKIF"
     gene            865..1566
                     /locus_tag="PLY81_06200"
     CDS             865..1566
                     /locus_tag="PLY81_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNF29853.1"
                     /translation="MLFYAKTYLADFLHLIFPNYCEGCGTDNITEDHFLCIKCIAALP
                     VTGFENVYENTIEKMFYGRLNLQYATAGFYFSKQTLMQHLMHQLKYKGNKEAGLFLGK
                     MLGLQLLHSDKFKTIDAIIPMPISSKKIVVRGYNQAEIISQGITMVFNKPILKNVVVR
                     IINSKTQTKQNRVERWENMKGVFEIKNKIDLEGKHILLVDDIVTTGATLEACGKVILQ
                     NGNCTLSIATVAYTL"
     gene            complement(1584..2522)
                     /locus_tag="PLY81_06205"
     CDS             complement(1584..2522)
                     /locus_tag="PLY81_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF29854.1"
                     /translation="MDISIVIPLYNEEESLPELSEWIKRVTTTNNFSYEVIFIDDGST
                     DNSWNIIEKINANNNNFKGIKFQRNYGKSAALNEGFRAAKGTVVITMDADMQDSPDEI
                     PSLYKMVTEDNFDIVSGWKKKRFDPITKTIPTKFFNAATRWSSGIKLHDFNCGLKAYK
                     NKVVKSIEVYGEMHRYIPVIAKWAGFRKIGEKIVEHRTRKYGKTKFGWERFINGFLDL
                     ATISFVSRFSKRPMHFFGLYGTICFLCGFLMSIYLIISKLLISNFALTNRPAFFIALT
                     VMIIGMQLFLAGFIAELIGRNAPERNSYLIESRLDV"
     gene            complement(2531..3049)
                     /locus_tag="PLY81_06210"
     CDS             complement(2531..3049)
                     /locus_tag="PLY81_06210"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNF29855.1"
                     /translation="MEQKPLSVPVKGLIISLVLIVLGLIIHFAGLSQIKGLQLLQYVI
                     LGSGLIWACITYAKQNNGNVTFGNTFAHGFKVTAAITGIIAVYTFLAIKFIMPEMIDY
                     AIDEARKGMVEGNKMTAEQIEQALAMTRKFFLPFALAGVIVGFLVLGVIFSLIGAAVA
                     KKNPNYSPIQNQ"
     gene            complement(3058..4200)
                     /locus_tag="PLY81_06215"
     CDS             complement(3058..4200)
                     /locus_tag="PLY81_06215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF29856.1"
                     /translation="MSITGNSIYHITTLDNLKNILELKHFKINYCRETLETGSNAAIA
                     GGFTTVCIIPNTQPTIHSKSQVEYVVQKTKQLPINILPLGTLTQNAEGKILAEMYDMH
                     QSGAIAFTDGLHPVQIPGVLLKALQYVKSFNGTVIQMPIDKSIGGNGLMNEGIISTKL
                     GLPGLPSLAEEIIIKRDIELLKYTQSKLHITGITTAKSVALIKGAKAEGLAITCSVTP
                     YHLFYCDEDLQAYDTNLKVNPPLRTKADVVALQQAVLNGDIDCIATHHFPHHSDNKVC
                     EFEYAKNGMIGLQTAFAAINTALPNLSNELLVNLFYTNATDIFSLRKNSIKENQVANL
                     TIFTRDGNTTLNKENNKSKSFNTPFSNKTLKGKVLGVITKNIFHKN"
BASE COUNT         1509 a          697 c          704 g         1594 t
ORIGIN      
        1 attatagatt ctatacaaac attacaaagc aatttaatag atgcaggtgc aggtagtgta
       61 agccaaataa aagaatgtgc tgcagagttt caacgatttg caaaagaaac caatacaccc
      121 gtttttttaa ttggccatat aacaaaagat ggcaacattg cagggcctaa aattttagaa
      181 cacatggtag atactgtttt acaatttgaa ggagacagac attatgccta cagaatttta
      241 agaactatta aaaatagatt tggaagcact gcagaacttg gtatttatga aatgacagga
      301 gaaggcatga gggcagtaaa taacccaagc gaaattttaa tttctcaacg agatgatcaa
      361 ttaagtggta ttgcaattgc tgcaactata gaaggaatga gaccattgct gatagaagtg
      421 caagcattgg ttacacaaag tgtttatggc accccacaac gtactgttac aggctttgat
      481 ttgcgtaggt tacaattgtt acttgctgtt ttagaaaaac gaggagggtt tttatttggc
      541 acaaaagatg tttttgtaaa cattgcagga gggttaaaaa tagaagaccc atccattgac
      601 ttagcagtta tttgtgcttt actatccagt tatgatgata cgcctatttc acataaaatt
      661 tgctttgcag gagaagtggg attgaatgga gaaattagag ctgtaaacag aattgaacaa
      721 agaattgccg aagcagaaaa acttggattt gaaaaaatat ttgttagtaa attcaacaaa
      781 aaatcggtta acttaaaact atttaatata gatattatta tggtaagcca attgcacgaa
      841 gtttatcaaa aaatatttta gtgtatgtta ttttatgcaa aaacatattt agctgatttt
      901 ttgcatctta ttttccctaa ttattgcgag ggttgtggca ctgataatat tacagaagat
      961 cattttttat gtattaaatg tattgctgca ttgccagtta ctggttttga aaatgtgtat
     1021 gagaatacaa ttgaaaaaat gttttatggc agattaaatt tacaatatgc aactgctggt
     1081 ttttattttt caaaacaaac tttaatgcaa catttaatgc accaattaaa gtataaaggt
     1141 aataaagaag caggcttatt tttaggaaaa atgcttgggc tgcaattgtt acattcggat
     1201 aaatttaaaa ctatagatgc aattattcca atgcccataa gctcaaaaaa aatagttgta
     1261 agaggctaca atcaggcaga aattatttcg cagggcatta caatggtttt taataaacca
     1321 atattaaaaa atgttgtagt aagaattatt aattcaaaaa ctcaaaccaa acaaaatagg
     1381 gtagaacgtt gggaaaatat gaaaggtgtt tttgaaataa aaaataaaat tgatttagaa
     1441 gggaaacata ttttattagt agatgatatt gtaacaacag gtgctacttt agaagcatgc
     1501 ggtaaagtta ttttacaaaa tggcaattgc acattaagta ttgcaactgt agcttatact
     1561 ttatagtgta taaaaatgca gtattaaaca tctaatctac tttcaattaa ataactattt
     1621 ctttcaggtg catttcttcc aattaattcg gcaataaacc ctgctaaaaa aagttgcata
     1681 ccaattatca ttactgttaa tgcaataaaa aaagcaggac gattggttaa tgcaaagttg
     1741 cttattaaga gctttgaaat aattaaataa atgctcatta aaaatccgca taagaaacaa
     1801 attgttccat aaagtccaaa aaagtgcatt ggtcttttac taaatctgct aacaaagctt
     1861 atggttgcta aatctaaaaa gccgttaata aatcgttccc atccaaattt tgttttacca
     1921 tattttctag ttctatgctc aacaattttt tcgccaattt tcctgaagcc agcccattta
     1981 gcaattacag gaatatatct gtgcatttcg ccatatactt caatgctttt tactactttg
     2041 tttttatatg cttttaggcc acaattaaaa tcgtgtagtt taatgccact actccaacgt
     2101 gtggctgcat taaaaaattt tgttggaatt gttttagtaa tagggtcaaa gcgttttttc
     2161 ttccaaccgc taacaatatc aaaattatct tcagtaacca ttttgtataa agaaggaatt
     2221 tcatctggac tgtcttgcat atctgcatcc atagttatta caacagttcc tttagcagct
     2281 ctaaaacctt cgtttaaagc tgcactttta ccataatttc gttgaaattt tattccttta
     2341 aaattgttgt tgttagcgtt tattttttca ataatattcc aactattatc ggtgctacca
     2401 tcatcaataa aaattacttc ataactaaaa ttatttgtag tggttacacg ttttatccat
     2461 tcacttaatt caggtagcga ttcttcttcg ttgtataaag ggataacaat agaaatatcc
     2521 atatagtatt ttactgattt tgaatagggg agtagtttgg atttttctta gcaactgctg
     2581 caccaattaa agaaaatatt actcctaaaa ctaaaaagcc tacaataaca ccagctaaag
     2641 caaaaggtaa aaagaatttt ctagtcattg ccaatgcttg ttctatttgt tctgcagtca
     2701 ttttatttcc ttcaaccatt ccttttcttg cttcatcaat agcataatct atcatttctg
     2761 gcataataaa tttaatagct agaaatgtat aaactgcaat aataccagta atggcagcag
     2821 taactttaaa gccatgtgca aatgtgttac caaaagtaac gttaccatta ttttgctttg
     2881 cataagttat acatgcccaa attaaaccag accctaaaat tacatactgc aaaagttgca
     2941 atcctttaat ttgacttagg cctgcaaagt gtataattaa acctaataca attaatacta
     3001 aagaaatgat taatccttta acaggtacag atagtggctt ttgttccatt ttttatttta
     3061 gtttttgtga aaaatgtttt ttgtaattac gcccaatact tttcctttta aggttttgtt
     3121 tgaaaaaggg gtattgaatg atttgctttt attattttct ttatttaaag tagtatttcc
     3181 atctcttgta aatatagtta agttagcaac ttgattttct ttaatgctat tttttcttaa
     3241 actaaaaata tcagttgcat tagtataaaa taaatttacc agcagttcat tagataaatt
     3301 tggaagtgca gtattaatgg ctgcaaaagc tgtttgtaag ccaatcatac cattttttgc
     3361 atactcaaat tcacacactt tattatctga atgatgtgga aaatgatgtg tggcaataca
     3421 atcaatatct ccatttaaaa ctgcttgctg tagtgcaacc acatctgcct tagtacgcaa
     3481 aggagggttt acttttaaat ttgtatcata cgcttgcaag tcttcatcgc aataaaacaa
     3541 atgatatggt gtaacactac aagtaattgc caagccttct gctttagctc cttttataag
     3601 tgctacactt ttagcagttg ttatacctgt aatatgtagt ttactttggg tgtattttag
     3661 taattctata tctctcttaa ttattatttc ttctgctaaa cttggtaaac ctggtaaacc
     3721 taatttagtt gaaataattc cttcattcat tagcccatta ccaccaatac ttttatctat
     3781 tggcatttga ataacagtac cattaaaaga ttttacatat tgtaatgctt ttagtaaaac
     3841 accaggtatt tgaacaggat gtaaaccgtc agtaaatgca atggcaccac tttggtgcat
     3901 atcatacatt tcagccaaaa ttttgccttc tgcattttgg gttaatgtgc ctaatggcaa
     3961 aatatttatt ggtaattgct ttgttttctg aactacatat tccacttgcg attttgaatg
     4021 aatagttggt tgggtatttg gaataataca tacagttgta aatccacctg ctatagctgc
     4081 attacttcct gtttctaatg tttctctaca atagtttatt ttaaaatgct ttaattctaa
     4141 aatatttttt aaattatcta atgttgttat gtgatagatt gaattgcctg taatgctcat
     4201 aatttaatta tgttccttat accaaatata ataaaaaaag ctgcatgttt taaatgcagc
     4261 ttttttgtgt tgttcgtcat gctgaacttg tttcagcatc tctgagaccc tgaaacgagt
     4321 tcagggtgac aattacttcg cataagcagt agctcgtact tccctgatca ccgttacttt
     4381 tacttggcca ggatatgtca tttcagtttg aattttttgt gagatgtcaa aagccagttg
     4441 ttctgatttt ttatcatcta ctttatctgc cgatactaat acacgcactt cacgtcctgc
     4501 ttgg
//
LOCUS       DAOQHP010000165         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11360_length_4482_cov_3.062119, whole genome shotgun sequence.
ACCESSION   DAOQHP010000165 DAOQHP010000000
VERSION     DAOQHP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11360_length_4482_cov_3.062119"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLY81_06220"
     CDS             <1..1167
                     /locus_tag="PLY81_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29857.1"
                     /translation="INISNAKGQVDLIAKDFRLPKVFRASLAFDKRFDNGWSLTAEVI
                     LTKNINEIYYQNLNILPPTLKATGPDNRTVYAAGNASAPYIPINNNNTNPYTGIYLIS
                     NKKDKKGFAHNFTVTANKNFTNGLSFNASYAYGYSEVTYEGTSSQNNSQYNTLATVNG
                     RNFADRHRSNFDLGHRITAFLSKKFTYARKSMSTTVSLFYTGQSGNPLTYVYNGSMVR
                     DVNPTNNQDLIYIPTAADITNAVFVTSGGLTPAQQKAALETFISGNKYLSSRRGQYAE
                     NNGDRLPFTNVLDLSIKQDFNLKIGKNKYQIQVSYDIFNFSNMLNRDWGRQYFVNFNT
                     YSLMSFRGYQPNTTIPTYSFVPPASGSVHTVSTSTAPSFSARWVSQLGVRLSLF"
     gene            complement(1247..1852)
                     /locus_tag="PLY81_06225"
     CDS             complement(1247..1852)
                     /locus_tag="PLY81_06225"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNF29858.1"
                     /translation="MLLQKSKIDFGLLLLLAFNIYAIYYYYQNPNALGALIVIFWLQS
                     VFIGIFNVIGMLTFTNRVENSFTINDKAGNRSGCAALFFTFHYGLFHFAYMFFLISYI
                     KDITPSQFTFIKLSFWAILAGSIIQYLQDKARNRTQPVNIGNMFVLPYARIVPMHLVI
                     LLPKFLSISPSIVFVVLKLFADLIMHIAYKNFVFKKSNQSL"
     gene            1949..2665
                     /locus_tag="PLY81_06230"
     CDS             1949..2665
                     /locus_tag="PLY81_06230"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNF29859.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPNVVKAAINAQQFGADGVTVHP
                     RPDERHITYNDVREIKKIIITEFNIEGNPREDKFVKLVLETLPQQVTLVPDALGQLTS
                     DHGWNTIEHKNYLIDIIAIFKNAGIRVSIFVDPIEEMIAAAALTNTDRIELYTESYAK
                     QFSINKENAIAPYIKAANKAFDLGLGINAGHDLDLTNLNYFAKNIPQLNEVSIGHALI
                     SDALYMGFENTIQIYKRQLL"
     gene            2744..4144
                     /locus_tag="PLY81_06235"
     CDS             2744..4144
                     /locus_tag="PLY81_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNF29860.1"
                     /translation="MALSKIWSAFIITAIIVALCRMLFFDGGTNLFSKMVVGKHGDTT
                     NTKTIDSNHLSQTILATIKENKEFKNGETKYTKNKEGNYIVYNEQSADGILATCTVAV
                     NICIGLIGIMALFMGFLAIAEKAGAINFLSRIIGPFFNKLFPDLPKNHPAFGHMILNF
                     SANLLNMDNAATPFGIKAMQSLQEANPDKDKASNAQVMFLCLHASGLTLIPITIIAFR
                     AQLGAANPTDIFIPCMIATFAATMAALLIVSFKQKINVLQPIILMWVLGISAIIAALV
                     LYIKSLSKNEVDVFSGLLSNGLILLIFLLIVLGGLYKKVNVFDAFVEGAKGGFETAIK
                     IIPYLVGMLVAISLLRTSGTFDALINGMKHLFLAIGMDTRFVDGLPTALIKPMSGSAA
                     RGMMIDTMRIFGADSYAGRLSSILQGSSDTTFYVVAVYFGAVGIKNTRYTIGSMLLAD
                     LVGIITAIILSYMFFT"
     gene            complement(4146..>4482)
                     /locus_tag="PLY81_06240"
     CDS             complement(4146..>4482)
                     /locus_tag="PLY81_06240"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNF29861.1"
                     /translation="VHPAIIDAGNEWANQQISPYVIKEAALLFEAGSASDLDYVIGVY
                     APKIIRLQRVMQRDKVDKQAVLNRMSKQIDEEIKMKLCDFIITNDDEQMVIPQVLKLH
                     EKFLAMSNM"
BASE COUNT         1622 a          681 c          700 g         1479 t
ORIGIN      
        1 attaatatta gcaatgctaa aggtcaggta gatttaattg caaaagactt tagattacca
       61 aaagtattta gagcatcttt agcatttgat aaaagatttg ataatggatg gtcattaaca
      121 gccgaagtta ttttaactaa aaatatcaac gaaatttact atcaaaactt aaatatttta
      181 cctccaacat taaaagcaac aggaccagat aatcgtacag tatatgctgc aggaaatgct
      241 tctgctcctt atattcctat taataacaat aataccaacc cttatactgg tatatattta
      301 ataagcaaca aaaaagataa aaaagggttt gctcataact ttactgtaac tgcaaataaa
      361 aacttcacta atggtttatc atttaatgca agttatgctt atggatattc tgaagtaact
      421 tatgaaggta caagtagcca aaacaatagc caatataata cacttgcaac tgtaaatggt
      481 agaaattttg ccgacagaca tcgtagtaat ttcgatttag gacatagaat tactgcattt
      541 ttatctaaga aatttactta tgccagaaaa tcaatgtcaa ctacagttag cttattctat
      601 actgggcaaa gtggtaaccc tttaacctat gtttataatg gttcaatggt tagagacgtt
      661 aatccaacaa acaaccaaga tttgatttac ataccaactg ctgccgatat tactaatgca
      721 gtatttgtta cttctggtgg cttaactcct gctcaacaaa aagctgcttt agaaacattt
      781 atttctggta ataaatattt aagttctaga agagggcaat atgctgaaaa caatggtgat
      841 agattaccat ttaccaatgt attagacttg agtattaaac aagactttaa tttaaaaatt
      901 ggtaaaaaca aataccaaat tcaagttagc tacgatatct ttaacttcag caatatgttg
      961 aatagagatt ggggtcgcca atactttgta aactttaata cctattcatt aatgtcgttt
     1021 agaggttatc aacctaacac tacaatacca acctatagtt ttgtaccacc tgcttcaggt
     1081 tcagtacata ctgtaagtac aagtactgca ccttcgttca gtgctcgttg ggtaagccaa
     1141 ttaggtgttc gtttaagttt attttagtag gtactaaata ttcctataaa agaggttgtc
     1201 ttttagacaa cctcttttgt ttttagtgca actatttagt ttatttttaa aggctttggt
     1261 tacttttttt aaaaacaaaa ttcttataag caatatgcat aatcaaatcg gcaaaaagct
     1321 ttaacactac aaatacaata gatggagata tagaaagaaa tttaggtagc agtatcacca
     1381 aatgcatagg aacaatacgg gcataaggca aaacaaacat gttaccaatg tttacaggtt
     1441 gtgttctgtt tctagcctta tcttgaagat actgaataat gcttcccgct aagattgccc
     1501 aaaaagaaag ttttataaat gtaaattgag aaggtgtaat atcttttata taagaaatta
     1561 aaaagaacat gtacgcaaaa tgaaacaaac cataatgaaa agtaaaaaat aaagctgcac
     1621 aaccacttct attacctgct ttatcgttaa ttgtaaaaga gttttcaact ctattggtaa
     1681 aagtaagcat accaattaca ttaaaaatgc caataaaaac actctgtaac caaaaaatta
     1741 ctatcaatgc tcctaatgca tttgggtttt gataataata ataaatggca taaatgttaa
     1801 atgctaataa caataaaagt ccaaaatcaa tttttgattt ttgtaggagc ataaaaaaaa
     1861 ttttacttaa aggttataaa aaaattcaaa aaaacattac catttgcttt tgtaaatttg
     1921 tttttttata aatatataaa attacaaaat gactaaactt agtgtgaata taaacaaatt
     1981 tgcaacttta agaaacagtc gtggaggcaa taacccaaat gtggttaagg ctgcaataaa
     2041 tgcccagcaa tttggtgcag atggtgtaac tgtgcatcca agaccagacg aaaggcatat
     2101 aacttataat gatgtaagag aaataaaaaa aataattatc acagaattta atatagaagg
     2161 caacccaaga gaagataaat ttgttaagtt agtgctagaa acattaccac aacaggttac
     2221 acttgtacca gatgctttag gacaacttac ctccgaccac ggttggaata caattgaaca
     2281 taaaaactac ttaatagata ttattgcaat atttaaaaat gccggaatac gtgtatctat
     2341 ttttgtggac ccaatagaag aaatgatagc agcagcagcc ttaactaata cagatagaat
     2401 tgaactttac acagaatcat atgcaaaaca attctcaata aacaaagaaa atgcaatagc
     2461 accttatatt aaagcagcta ataaagcttt tgatttaggg ctaggaatca atgcaggaca
     2521 cgatttagac cttaccaacc ttaactattt tgcaaaaaat atcccacaac ttaatgaagt
     2581 aagcattggc catgcattaa taagcgatgc cttgtatatg ggctttgaaa ataccattca
     2641 aatttataag aggcagctat tgtaaccaaa tgttagtatt tagttttgtt ttcatttaca
     2701 taagtaaatt ttcttgctta ttttgttctc ataaaataaa aatatggcac taagtaaaat
     2761 ttggagtgca tttattataa ctgcaattat tgtagcctta tgcagaatgt tattttttga
     2821 cggaggaacc aatttattta gtaaaatggt tgtaggcaaa catggcgata caaccaacac
     2881 taaaactatt gatagcaacc atttatcaca gaccatactt gcaactatca aagaaaataa
     2941 agagttcaaa aatggcgaaa caaaatacac taaaaacaaa gaaggcaatt atattgtgta
     3001 taatgaacaa agtgctgatg gtattttagc aacttgtact gttgcagtaa atatttgtat
     3061 tgggttaata ggaataatgg ctttgtttat gggctttttg gctattgcag aaaaagcagg
     3121 tgcaattaat tttttatctc gaattattgg tccatttttt aataaactat ttcccgattt
     3181 acctaaaaac caccctgcat ttgggcacat gattttaaac ttttctgcta accttttaaa
     3241 catggataat gcagccacac cttttggcat taaagccatg caaagcttac aagaagcaaa
     3301 tcccgataaa gataaagcat ctaacgcaca ggttatgttt ttatgccttc atgcaagtgg
     3361 attaacttta atacctataa caattattgc ttttagagct caattaggtg ctgccaaccc
     3421 aacagatatt tttatacctt gtatgattgc aacttttgct gctaccatgg cagcattatt
     3481 gattgtatct tttaaacaaa aaattaatgt attacagccc ataattttaa tgtgggtatt
     3541 aggtattagt gcaataattg cagcattggt tttatatata aaatctttaa gtaaaaatga
     3601 ggtagatgtt ttttcaggtt tgttaagtaa tggtttaata cttttaattt ttctattaat
     3661 agtattaggt ggtttataca aaaaagtaaa tgtttttgat gcatttgtag agggagcaaa
     3721 aggaggtttt gaaacagcaa taaaaattat tccatattta gtgggtatgc tagttgcaat
     3781 tagcttacta cgtactagcg gcacttttga tgcattaata aacggaatga aacatttgtt
     3841 tttagctata ggtatggata ctcgttttgt agatggttta ccaacagcat taataaaacc
     3901 catgagtggc agtgctgcaa gaggaatgat gattgatact atgagaattt ttggagctga
     3961 ttcttacgca ggaagattaa gcagtatttt acaaggctct agcgatacta ctttttatgt
     4021 agtggctgta tattttggtg ctgtaggtat taaaaataca cgttatacaa ttggctcaat
     4081 gttattggca gatttagttg gaattattac tgcaattatt ttaagctata tgttttttac
     4141 ttaagctaca tattactcat agctaaaaat ttttcatgca attttaatac ttgtggtata
     4201 accatttgtt cgtcatcatt cgttataata aaatcgcata gtttcatttt aatttcctca
     4261 tctatttgtt tactcattct atttaaaaca gcttgcttat ctaccttatc tctttgcatt
     4321 actctttgca atcgtattat tttgggtgca taaactccaa ttacataatc taaatccgat
     4381 gcactacctg cttcaaataa caaagcagct tctttaataa cataaggcga aatttgttga
     4441 ttagcccact catttccagc atcaatgatg gcaggatgaa ca
//
LOCUS       DAOQHP010000166         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11492_length_4451_cov_3.984759, whole genome shotgun sequence.
ACCESSION   DAOQHP010000166 DAOQHP010000000
VERSION     DAOQHP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11492_length_4451_cov_3.984759"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1045)
                     /locus_tag="PLY81_06245"
     CDS             complement(245..1045)
                     /locus_tag="PLY81_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29862.1"
                     /translation="MKLKLLISLMVIQFTVIKSVNAQEINSVTFLKEFNELFSDAQDG
                     FPKSKGSLLEDIGWFGRKYAANKYFFNNSAMAALQYIKPHEPTKYVPKATPEQFYFTQ
                     TFIVERNEGKFANDSLEIVLDLIAKGGGYYKKNIKQEKEYRKTYKSIEYQKSNKPIYS
                     LLKNLKSNTYSITIYSPVRPSDVAKPKNRLGCIVFKYTGFSYVYAVTVYGEKLSNVNN
                     IVEKAFKATGLVDRYNSYFWHPKKSVDQISIELGNSVIVRDAGSIDVD"
     gene            1181..2860
                     /locus_tag="PLY81_06250"
     CDS             1181..2860
                     /locus_tag="PLY81_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF29863.1"
                     /translation="MTNSEIADNFSLLSKLMDINRENSFKAKSYSSAAFTIDKLPVQL
                     SSLEQNKIFSLKGIGESVGQKIIEQINNGFLQTLNEYILKTPIGVREMLNIKGIGPKK
                     IATIWHEMEIESIGELLYACNENRLTLFKGFGEKTQQTIKENIEFYLNTFGNYLYQQI
                     ENYAIAFHQKLQQTFLHSEFIFTGSFRRQIEVMDKVEWVTNVEVNELKIFFKSNNYNI
                     IQENEGYIIFKGIENVELCFYLTDVSFLFNQLFATSCSNHFLEEWISKTGWQITDPYE
                     SEEEIFSLNNCNYIPAYQRETAEIIALAFKQPLPKVIETRDIKGIIHSHSNWSDGVNT
                     IEEMANACMLKGFEYLVISDHSKTAFYAQGLSVERVLVQHILIDELNKKLYPFKIFKS
                     IESDILNDGSLDYEPEILNKFDLVIASIHSNLKMNEEKAMHRILGAVTNPYTSILGHL
                     TGRLLLSRNGYPVNYSTLFEACKKHRVVIELNAHPRRLDMDWRYINDAVDNNILISIN
                     PDAHAIEGFGDCKYGVLIAQKAGLTTNNNLSSFSLQQMNTFIETQKLKRRG"
     gene            complement(2940..3674)
                     /locus_tag="PLY81_06255"
     CDS             complement(2940..3674)
                     /locus_tag="PLY81_06255"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HNF29864.1"
                     /translation="MKKIFLIIFLLPFLSNAQVLGGNNVIKANLSSLAFGNYHLTYER
                     ALLKKLSISVSYRYMPKKTVPLSNLLEKYIDNKDVNFGSFEMGNTAITPELRLYPVKK
                     LKGFYLAAYGRFADFDLSVPVRYVASSLPGSPTEYATFNGKIKSSSIGFMIGTQFRLA
                     KKLVLDWWIIGGHYGRSKGDLNALINRNLSPIEQQSLQATLDDLKEIGPFKFEGKVTS
                     PNTANVKSTGPWAGVRSMGLSLGVRF"
     gene            complement(3807..>4451)
                     /locus_tag="PLY81_06260"
     CDS             complement(3807..>4451)
                     /locus_tag="PLY81_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNF29865.1"
                     /translation="KEWQTLWWDKVSNSLPPNCTPEESWNRKAGILAEFGKIICISTA
                     YFYENENRNQCLKIKSIYGHNEVDVLNQFILLCSKMYKHNKNFIYCGHNIKEFDIPYI
                     CRRLMINNLPIPEYLWLHDRKPWDVNMFDTLNWWKFGDNKNYISLHLLASVLNIPTSK
                     SDIDGSKVQEVYYKEKNLQRIVEYCQKDVVVCANIVLKYKGIPLLSNESIFITN"
BASE COUNT         1598 a          630 c          630 g         1593 t
ORIGIN      
        1 aaatctgatg atataattgg ccgtgcaaaa acttatgaag ctattgtaaa aggcgataat
       61 attccaaaag ctggagtacc agaatctttt aatgtattag tacatgaatt aagaggttta
      121 ggcttagatt taaattttga gcagtaaata aattttaatt caatatttaa aaactcctat
      181 ataaaaaaat ataggagttt ttttattttg tttaagaaaa tataagctat ataagaaaga
      241 ttttttaatc cacatcaata cttcctgcat ctctaacaat aacactatta cctaattcaa
      301 tagaaatctg atctactgat tttttgggat gccaaaaata ggagttgtat ctatccacta
      361 gccctgttgc tttaaaagct ttttcaacta tattatttac attacttagt ttttctccat
      421 atactgtaac tgcatatacg tatgaaaatc ctgtatattt aaacacaata cagcctaatc
      481 tgtttttggg ctttgcaaca tcactgggtc taacaggaga ataaatagtg atggaatatg
      541 tattgctttt aagatttttt agcaaagagt atattggttt attgcttttt tgatattcta
      601 ttgatttgta tgtttttcta tactctttct cttgcttaat atttttttta taatatcctc
      661 ctccttttgc tattaaatct aaaactattt ctaaagaatc gttggcaaat tttccttcat
      721 ttctttctac aataaatgtt tgtgtaaaat aaaactgctc tggtgttgct ttaggaacat
      781 actttgtagg ttcgtgtggt ttaatatatt gtaatgctgc catagcagaa ttattaaaaa
      841 aatatttatt ggctgcatat ttcctaccaa accaaccaat atcttctaac aaactacctt
      901 tacttttagg aaaaccatct tgtgcatcag aaaaaagttc attaaattct tttagaaagg
      961 taacagaatt tatttcttga gcatttacac ttttgattac tgtaaactga attaccatta
     1021 aactaattaa taactttaat ttcataatta atatttaaaa aggtataaca atatattatt
     1081 cttttatttc gtaaaataaa tacatgttac attttataga taattgattg aaatattttt
     1141 ttaaaaatac ttaatttgtt caacacaaac atttattata atgaccaatt cggaaatagc
     1201 tgataacttt tctttactat ctaaactaat ggatattaat agggagaata gttttaaagc
     1261 aaagtcatat tcctcggctg cttttactat tgataaacta cctgtacaac tttcttcttt
     1321 agagcaaaat aaaatttttt ctttaaaagg aattggcgaa tctgttggac aaaaaattat
     1381 agaacaaata aataacggct ttttacaaac tcttaatgag tatattttaa aaacacccat
     1441 tggggtaaga gaaatgctta atataaaagg cattggcccc aaaaaaattg ctacaatatg
     1501 gcatgaaatg gaaattgaaa gtattggcga attgctttat gcttgtaatg aaaatagatt
     1561 gactttattt aaaggttttg gcgaaaaaac acaacaaacc ataaaagaaa atattgagtt
     1621 ttacttaaac acttttggca attatctgta tcaacaaata gagaattatg caattgcatt
     1681 tcatcaaaaa ttgcaacaaa catttttaca ttccgaattt atatttacag gttcttttag
     1741 aagacaaata gaagtaatgg ataaagtaga atgggtaacc aatgtggaag ttaatgagtt
     1801 gaaaattttt tttaagtcaa ataattataa cataattcag gaaaatgagg gctatataat
     1861 ttttaaagga attgaaaatg tagaactttg tttttattta accgatgttt cgtttttatt
     1921 taaccaactt tttgcaacaa gttgcagtaa tcatttttta gaagaatgga taagtaaaac
     1981 aggctggcaa ataacggacc cttatgaatc agaagaagaa attttttctt taaacaattg
     2041 caattatata ccagcttacc aaagagaaac tgcagaaata attgcattag cctttaagca
     2101 gccattaccc aaagtaatag aaacaagaga tataaaaggc atcattcata gccatagtaa
     2161 ttggagtgat ggtgtaaata ctattgaaga aatggctaat gcctgtatgc taaaaggctt
     2221 tgaatattta gttattagcg accatagcaa aacagctttt tatgctcaag ggttaagcgt
     2281 agaaagagtt ttagtacaac atattttgat agatgaatta aataaaaaac tttatccctt
     2341 taaaatattt aaaagtatag aaagcgatat tttaaatgat ggcagcttag attatgagcc
     2401 agaaatttta aataaattcg atttggtaat agcttctata cattctaatt taaaaatgaa
     2461 tgaagaaaaa gcaatgcatc gtattttagg tgctgttacc aatccatata cttctatttt
     2521 aggacattta acaggcaggt tattattaag tagaaatgga tatccggtga attattctac
     2581 tttatttgaa gcatgtaaaa aacaccgggt agtaatagaa ctaaatgctc accctagaag
     2641 attagatatg gattggcgat acatcaatga tgcagtagat aataatattt taatttctat
     2701 aaaccctgat gctcatgcta ttgaagggtt tggagattgt aaatatggtg tgctaattgc
     2761 acaaaaagca gggctaacaa ctaataataa tttaagtagc tttagtttac agcaaatgaa
     2821 cacttttata gagactcaaa agttaaaacg tagaggataa aaattttaga ggctatatta
     2881 gtcataataa aataaggcag cccttgggct gccttatttt attatgaaat tttatttatt
     2941 taaaacctaa cacctaaact taaacccata cttctaacac ctgcccatgg gcctgtagat
     3001 tttacattag ctgtatttgg cgaggttact ttaccttcaa atttaaaagg gccaatttct
     3061 tttaagtcat ctaaggtagc ttgaaggctt tgttgctcta taggagaaag atttctgtta
     3121 attaaagcat tcaaatctcc tttgcttcta ccatagtggc cacctataat ccaccagtca
     3181 agtactaatt tttttgcaag cctaaattgt gtaccaatca taaaaccaat acttgacgat
     3241 ttaatttttc cgttaaaagt agcatattct gttgggctgc caggtaatga gctagcaaca
     3301 tatcttacag gaacacttaa atcaaaatct gcaaatctgc cataggcagc taaataaaat
     3361 ccttttaatt tcttaacagg atatagtctt aactctggag taatagcagt attacccatt
     3421 tcaaaacttc caaagttaac atccttattg tctatatatt tttctaataa attagataaa
     3481 ggaacagttt ttttaggcat atatctgtaa gaaacagaga tagaaagttt tttaagtaat
     3541 gctctttcgt aagttaaatg gtaattgcca aaagctaaag aagataagtt ggctttaata
     3601 acattgttgc cacctaaaac ttgtgcatta gataaaaacg gtaaaagaaa aataattaag
     3661 aatatctttt tcatggttta aattttaaat tattttgtat ttaaatggtt atttataata
     3721 atttataaat aaagttaacg attattaatt gcaatgataa tttttttttc aataagccaa
     3781 atacccaatt aatggtattt tatgttctaa tttgtaataa atatactttc attactaagc
     3841 agtggtatgc ccttgtactt taaaactata ttagcacaaa ctacaacatc tttttggcag
     3901 tactcaacaa ttcgttgtaa gtttttttct ttgtaataaa cttcttgcac tttactacca
     3961 tctatatcgc ttttacttgt aggaatattt aaaacagaag ctaataagtg taaggatata
     4021 tagtttttat tatcgccaaa tttccaccaa tttaaagtgt caaacatatt tacatcccaa
     4081 ggctttctat catgtagcca aagatattcg ggtatgggta agttattaat cattaacctg
     4141 cggcatatat aaggtatatc aaattcttta atgttatgac cacagtaaat aaaattttta
     4201 ttatgcttat acattttgct acacaataaa ataaattggt ttaaaacatc tacctcattg
     4261 tggccataaa tactttttat ttttaaacac tgatttctat tttcattttc ataaaaataa
     4321 gctgtactta tacaaataat ttttccaaac tctgctaaaa tacctgcttt tctattccag
     4381 ctttcttcgg gtgtgcaatt tggtggtaac gaattagaaa ccttatccca ccatagtgtt
     4441 tgccattctt t
//
LOCUS       DAOQHP010000167         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11560_length_4436_cov_3.658297, whole genome shotgun sequence.
ACCESSION   DAOQHP010000167 DAOQHP010000000
VERSION     DAOQHP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11560_length_4436_cov_3.658297"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..3108
                     /locus_tag="PLY81_06265"
     CDS             349..3108
                     /locus_tag="PLY81_06265"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNF29866.1"
                     /translation="MKDFSYITNSHPSFIENLYNEFLKDPNSIDPDLKKFFEGFDFAV
                     NFGNFNQIISSDQTSSATTSSSQNIDWMNEIKVYRLILGYRNKGHLLANTNPIRPRKD
                     RGANLDLNFFGLSDADLNQTFYAGNLIGLGPTTLKNILEYLQKIYANDVGIEFKYISN
                     QNKIDWLVNEIEQKFSSPLSLNKKKRILEKLNEGVMFEKFLHTKYIGQKRFSLEGGEN
                     TITALDQIITTAANNNVEEVVIGMAHRGRLNVLANIMGKTYEQIFSEFEGTGSIDQTM
                     GSGDVKYHMGFGSKVTTEDGKEVHLKLMPNPSHLEAVNPVVEGFSRAKADILYNSEYK
                     KILPILIHGDSSVAGQGIVYEVLQMSELEGYDTGGTIHFVINNQIGFTTDFDDARSAD
                     YCTSIAAMVQAPVLHVNGDSAEAVIKCAEIATRYRQQFNSDIFIDMVCYRKHGHNEGD
                     DPKYTQPKLYALIDKHANPREVYVKHLLDNGESDAQNLAKEMEKKFWADLQERLDEVK
                     QKPLPYNYQQSELAWKSLRRAKAEDFDFSPSTAISDTEIRRLFNGLMHIPSNFQPLKK
                     IEKLLFDKVKLLETENKIDWATAELLAYSSILIEGKIVRMSGQDVKRGTFSHRHAVIR
                     DEVTDEFYNRLNSIQPNQEKFRIYNSLLSEYGVLGFEYGYAMANPNALTIWEAQFGDF
                     CNGAQTMIDQFIASSEQKWNRMNGVVMLLPHGYEGQGPEHSSARLERFLQLSAELNMV
                     ITNITTAANLFHVLRRQLAWEFRKPLINFSPKANLRYHGSYSDINEFTSGGFKEVIDD
                     ASVQDSSQIQKVLLCSGKIYFELAERKEKENRNDIAIIRLEQIYPTPEKQLKALHNKY
                     NKAIWFWIQEEPLNMGAATFLKMNLQSINFGIISRHASASTATGYSKVHKTEQEEIIS
                     TAFSI"
     gene            3147..4358
                     /gene="odhB"
                     /locus_tag="PLY81_06270"
     CDS             3147..4358
                     /gene="odhB"
                     /locus_tag="PLY81_06270"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNF29867.1"
                     /translation="MIDIKVPTVGESITEVTLLKWVKKDGAYVERDEVIAELESEKAT
                     FEVNAEQSGILKHGAAEGDTILIGNSMAQIDESAEKPTSSEPEKQQEKPAQPTNQAPV
                     TSIPNDVKATPVASAIIADKKVDVKAIEPSGFGGKILKNDVLEALSNPGKKAFGGNEL
                     FTRNVRNEKMSNLRKTISRRLVEAKNTTAMLTTFNEVDMGPIMDIRKQHKDKFKEIHG
                     VNLGFMSFFAKACAIALSEWPAVNAFIDGDVLVYHDYADISIAVSTPRGLTVPVMRNV
                     ESLSMADIEKKVIELAGKARDSKLTTDELTGGTFTITNGGVFGSLMSTPIINIPQSAI
                     LGMHKIQDRPMAINGKVEVRPMMYLALSYDHRIIDGRESVSFLVRVKELLENPSLLLI
                     GKDPVKTLLEL"
BASE COUNT         1688 a          650 c          796 g         1302 t
ORIGIN      
        1 aagttggggt accccaactt tatgaagact tacacagttt ataagatagt accgtcatgt
       61 tgaacgaagt gaaacatctg cttgttcctt taatgttatt cagaagatcc tttgctccac
      121 tcaggatgac ttaaagaaat gctcagtatt acactcatag taggaatgac ctcctatttg
      181 aacatataaa tatataaata atcaatttaa catcaaagag ctaattctta gaaaatccct
      241 taatattgat taaagaatta ttagtacaaa actttagttt ttttatggcc tcaaagcccg
      301 atatttgcca acaaatctat ctataattaa gtttaatttc aaccaataat gaaggatttt
      361 agttacatta ccaattccca cccaagtttt attgaaaatt tatacaatga atttttaaaa
      421 gaccctaata gcattgaccc tgacttgaaa aaattttttg aaggttttga ctttgctgta
      481 aattttggaa attttaatca aattattagt tctgatcaaa catcttctgc taccacttca
      541 tcttctcaaa acattgattg gatgaatgaa ataaaggttt ataggcttat attaggttat
      601 agaaataaag gccacttatt agcaaatacc aaccccatta gaccaagaaa agacagaggt
      661 gcaaatttag atctaaactt ttttggttta tccgatgcag atttaaacca aactttttat
      721 gcaggtaatt taattgggtt aggccccacc acattaaaaa atattttaga atacttacaa
      781 aaaatttatg ccaatgatgt tggtattgaa tttaaatata tcagcaatca aaataaaatt
      841 gattggttag tgaatgaaat agaacaaaaa ttcagctctc cactatcttt aaataaaaag
      901 aaaagaattt tagagaaatt gaatgaaggg gttatgttcg aaaaatttct tcatacaaaa
      961 tacataggtc aaaaaagatt ttcattagaa ggtggcgaaa atactattac agcattagac
     1021 caaattatta caactgcagc caataataat gttgaggaag tggtaattgg catggcacac
     1081 agaggcagat taaatgtact tgctaatatt atgggtaaaa cctacgaaca aatttttagt
     1141 gagtttgaag gtacaggatc aatagatcaa accatgggta gtggagatgt aaaataccac
     1201 atgggatttg gcagtaaagt taccacagaa gatggaaaag aagttcattt aaaactaatg
     1261 ccaaacccat cgcatttaga agctgtaaac cctgttgtag aaggattttc aagagcaaaa
     1321 gccgatatac tttacaatag tgaatataaa aaaatattac ccatattaat tcatggcgac
     1381 tcatcggtag ctgggcaagg aattgtatat gaggtattac aaatgagtga attagaaggt
     1441 tacgatacag gtggcactat acactttgtt attaataacc aaatagggtt tactacagat
     1501 tttgatgatg ccagaagtgc agactattgt acatctattg ctgcaatggt acaagcccct
     1561 gttttgcatg taaatggcga tagtgccgaa gctgtaataa aatgtgcaga aattgcaact
     1621 cgttatcgtc aacaatttaa cagcgatatt tttatagata tggtttgtta tcgtaaacat
     1681 ggccataatg aaggcgatga ccctaaatac acccaaccaa aactttatgc attaattgat
     1741 aaacatgcaa atccaagaga agtgtatgtt aaacatttat tagacaatgg cgaatctgat
     1801 gcacagaatt tagcgaaaga aatggagaaa aagttttggg cagacttaca agaaagatta
     1861 gacgaagtaa agcaaaagcc tttaccatac aactatcaac aatctgaact tgcatggaag
     1921 agtttaagaa gagccaaagc agaagacttt gacttctctc cttctacagc aattagtgat
     1981 accgaaatta gaaggttatt taatggttta atgcatattc catctaactt tcagccctta
     2041 aaaaaaatag agaaattatt attcgataaa gtaaaactat tagaaactga aaataaaata
     2101 gattgggcaa cagccgaatt actagcttat tcctctattt taattgaagg aaaaattgtg
     2161 agaatgagtg ggcaagatgt taaaagaggc actttctctc atcgtcatgc agtaattaga
     2221 gatgaggtaa cggatgagtt ttataatcgt ttaaactcca ttcaaccaaa tcaagaaaaa
     2281 tttagaattt ataattcttt attaagtgaa tatggtgttt tagggtttga atatggatat
     2341 gcaatggcca acccaaatgc tttaactatt tgggaagccc agtttggaga cttttgtaat
     2401 ggtgctcaaa caatgataga tcaattcatt gcatctagtg aacaaaaatg gaatagaatg
     2461 aatggtgtag ttatgctttt gcctcatggc tacgaagggc aagggcctga gcatagcagt
     2521 gcaagactag aaaggttttt acaactctct gcagagctga acatggttat taccaatatt
     2581 accactgcag caaatctttt tcatgtttta agaagacagt tggcttggga gtttaggaaa
     2641 cctcttatca atttttcacc aaaagctaat ttacgttatc acggaagtta tagtgatata
     2701 aacgaattta cttcaggtgg ttttaaagaa gtaatagatg acgccagcgt tcaagattct
     2761 tctcaaatac aaaaagtatt actatgctct ggtaaaattt attttgaatt agctgaaaga
     2821 aaagaaaaag aaaacagaaa tgatattgcc ataattaggt tagaacaaat ttatccaaca
     2881 ccagaaaaac aattaaaagc attacataat aaatataaca aagctatttg gttttggatt
     2941 caggaagagc ctttaaatat gggtgcagca accttcttaa aaatgaactt acaaagtatt
     3001 aattttggta ttattagtag gcatgcaagt gcaagcacag ctactggcta ctccaaagtt
     3061 cataaaaccg aacaagaaga aattataagt accgctttta gtatttaaca aaaacagaaa
     3121 taatataaat ttagaaataa atatttatga tagacattaa agtacctacc gttggagaat
     3181 caattactga agttacctta ttaaaatggg taaaaaaaga tggagcctat gtagaaagag
     3241 atgaagttat tgcagaatta gaaagtgaaa aagcaacttt tgaagtaaat gctgaacaat
     3301 cgggtatttt aaaacatggt gcagcagaag gtgatacaat tttaattggc aattcaatgg
     3361 cacagataga tgaaagtgct gaaaagccta cttcttcaga accagaaaaa cagcaagaaa
     3421 aacctgcaca accaaccaac caggcaccag taactagcat accaaacgat gtaaaagcta
     3481 caccagtagc aagtgctatt attgctgata aaaaagtaga tgtgaaagca attgaaccaa
     3541 gcggctttgg aggtaaaatt ttgaagaacg atgttttaga agcattaagt aacccaggta
     3601 aaaaagcatt tggcggtaac gaattattta caagaaatgt acgcaacgaa aaaatgagca
     3661 atttgcgtaa aactatttca agaagattag tagaagccaa aaacacaact gcaatgctta
     3721 ccacttttaa tgaagtggat atggggccta ttatggatat tcgtaaacaa cataaagata
     3781 agtttaaaga aatacatgga gtaaaccttg gttttatgag cttttttgca aaagcctgtg
     3841 ctattgcatt aagtgaatgg cctgccgtaa atgcttttat agatggcgat gtacttgtgt
     3901 accacgatta tgcagatatt tctattgctg taagcacacc aagaggttta acagtacctg
     3961 taatgagaaa tgtagaaagc ctaagtatgg cagatattga aaagaaagtt attgaactag
     4021 caggcaaagc aagagatagt aaactaacaa cagatgagct tactggtgga acatttacca
     4081 taaccaatgg tggcgttttt ggtagcttaa tgagcactcc aattattaat attccacaaa
     4141 gtgctatttt aggaatgcat aaaatacaag acaggccaat ggctattaat ggtaaagtag
     4201 aagttaggcc aatgatgtat ttagcattaa gctacgacca tagaattatt gatggaagag
     4261 aaagtgtaag ctttttagtt agagtaaaag aattattaga aaacccatcc ttattactaa
     4321 ttggcaaaga ccctgtaaaa actttattag aattataaaa acattagatg tataaaaaaa
     4381 gagtgagtaa taattgtaat tactcactct tttaatttta agattaaaat ttattt
//
LOCUS       DAOQHP010000168         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11651_length_4416_cov_3.555148, whole genome shotgun sequence.
ACCESSION   DAOQHP010000168 DAOQHP010000000
VERSION     DAOQHP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11651_length_4416_cov_3.555148"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLY81_06275"
     CDS             complement(<1..434)
                     /locus_tag="PLY81_06275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNF29868.1"
                     /translation="MTKRISFSLLILLTLLATCIFSFKSKDAQQPIQKQKLRKIVIDA
                     GHGGRDIGAEGKYSNEKTISLDVALKLQKTIEQEIPDVEVYMTRTSDFYDSPITKADK
                     ANAAKGDLFISIHCNSASGNRHRELVGYKTVTSKNRKTKKTTT"
     gene            505..3174
                     /locus_tag="PLY81_06280"
     CDS             505..3174
                     /locus_tag="PLY81_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNF29869.1"
                     /translation="MSKLYKINKKILTVFIVSIALFFLTLSLEANVVINNIAHPPIQD
                     TIPSKVKIGKDSIQNKKTDSTKVLTKDTLVFSKDSIDAPVKYSATDSGVLIISTKQFI
                     LYGSATAKYQDMELEAATIEYLQDKQTIKAKGSSDTSKNSLHKTKFKQAEMSTQSDSI
                     AYNLKTQKGITYNTYYQEGELFVNAGKLKKADSNIVYAYKGSFTTCNLDTPHFSFRTK
                     KMKIINNKIGVSGPAHPEFEGVPIPIYIPFGIYPLNRGRKSGILTPTFTSSEDFGLGF
                     EGIGYYKVLNDNLDITTRANFYTYGGWSITANSKYLKRYRYAGNVNIAVQKNKTLNRY
                     ATKSEDEFTSSSSYMINWSHSRDSKARPGTNFTANLNFGSTRFNKNILNNPYQNFNNQ
                     LSSSISYSKNWNSKYNLSLNLNHNQNNKTHLVNLSLPNANFNVVTFYPFEKKEAIGTA
                     KWYEKISIGYSGNLQNRISFYDTAFSSYKLLDTLQWGAEHNIPIGLALPALGPINIAP
                     SISFQQRWFGQKTHRYWSMADSLVKTSTNKGFYLQNSMSFGISASTRIFGTYQFKKGK
                     VKAIRHEIRPSFGINYTPDLVSKNFYNVQVDTSGRTMRFSEFDGGIYSSYNEGNFGGI
                     TFGIDNLLEMKVRDKTDTSKNADRKIKLIDGFGFSSSYNLLADSFALGSFNLYVRSTL
                     FDKINITASASLDPYQVNKFGQRVNAFMLKKGKLGRITNGSLAISTNFQSKSKDGKNE
                     EDSKNGIPNDPFLTPDEQQRQLDYIRQNPAEYTDFNIPWKVDLSYSLSFYQAIKPDYS
                     GYNTETFSSLNFNGDFSLSPKWKVGATGYYDLKYMNFQQFSMFISREMHCWQLSVNIT
                     PVGLWRSFNIVISPKSGILRDLKINRNRTFSNL"
     gene            complement(3164..4405)
                     /locus_tag="PLY81_06285"
     CDS             complement(3164..4405)
                     /locus_tag="PLY81_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNF29870.1"
                     /translation="MHTKHLSILDFSYPLSEERIAKFPLSLRDTSKLLVYNNEEISQD
                     YYYNLHNHIPENSLIIFNNTKVVEARLLFEKTTGSVIEIFCLEPDERYGDITVAMMQK
                     SKVYWKCLVGGAKKWKEDFLLKELKEDGNNIQLQAKIIEKKNDYFLIEFCWNNNELCF
                     SELLHLFGVIPLPPYIKRSTTEQDRVTYQTIYAKFDGSVAAPTAGLHFTDVMVKNLKA
                     KNIQPIYTTLHVGAGTFKPVKALTMQEHEMHAEYIEVTRQFIEQLLSKDFPTIIAVGT
                     TSLRTIESLYWMGVKIKTQTNFTSFNEIAINQWDAYELPQEILVNDALNSLLQWMIEN
                     NKEKIITKTQIIIAPGYNLKVADALVTNFHQPQSTLLLLVAAIAGNNWKKIYHFALEN
                     DFRFLSYGDGSLIWKNKKTTD"
BASE COUNT         1552 a          655 c          672 g         1537 t
ORIGIN      
        1 gtagtagttt tttttgtttt tctattttta cttgtaacgg ttttgtatcc tactaattct
       61 ctatgcctgt tgccacttgc agagttgcaa tgaatagaaa taaataaatc accttttgca
      121 gcattggctt tatcggcttt ggtaattgga gaatcataaa aatctgaagt acgagtcatg
      181 tacacttcta catctggtat ttcttgttct atggtttttt gtaattttaa agccacgtct
      241 aacgaaattg ttttttcatt ggaatatttg ccttctgcac caatatctct tccaccatga
      301 cctgcatcaa ttactatttt tcttagcttt tgtttttgta ttggctgttg tgcatcttta
      361 gatttaaaag aaaatataca ggttgccaat aaagttaata aaattagtaa tgaaaatgaa
      421 atccgtttag tcatatcaaa tagttaaata caagtgtttg tatgatgttt aaagtattag
      481 ctcttatttt tgctcagtta tactatgagc aagttgtaca aaattaataa aaaaatactt
      541 actgttttca ttgtaagtat tgctttgttt tttttaacat tatcattaga agccaatgta
      601 gtgattaata atattgctca tcctcctatt caggatacaa tcccttctaa agtcaaaatt
      661 ggcaaagatt ctatacaaaa taaaaaaaca gatagcacta aagttttaac aaaagatact
      721 ttagttttct ccaaagactc tatagatgct ccagtaaaat atagtgcaac agattctgga
      781 gttttaatta tatctaccaa acagtttatt ttatatggtt ctgcaactgc caaatatcag
      841 gatatggagc ttgaggctgc aacaatagaa tacttgcagg ataagcaaac tattaaagca
      901 aaaggttctt ctgatacatc taaaaactct ttgcataaaa caaagtttaa gcaagcagaa
      961 atgagtacac aaagcgattc tattgcatat aacttaaaaa cgcaaaaagg tattacttat
     1021 aatacttatt atcaagaggg agaattattt gttaatgcag ggaaattaaa aaaagcagat
     1081 agtaatattg tttatgctta caaaggtagt tttacaacct gtaatttaga tacaccacat
     1141 tttagtttta gaactaaaaa aatgaaaata attaataata aaattggtgt tagtgggcct
     1201 gcacatcctg agtttgaagg tgtaccaatt cctatttata ttccttttgg aatttatcct
     1261 ttaaatcgtg gtagaaaatc gggtatttta accccaactt ttaccagtag cgaagatttt
     1321 ggattaggct ttgaaggtat tggctattac aaagttttga atgataattt agatattaca
     1381 acaagggcta atttttatac ttatggtgga tggagtataa ctgcaaatag taaatattta
     1441 aaaagatacc gatatgctgg caatgtaaat attgctgtgc aaaaaaataa aacgctaaat
     1501 cgttatgcta caaaatcgga agatgaattt actagctctt cttcatacat gattaattgg
     1561 agccatagca gagatagtaa agcaaggcca ggtacaaatt ttacagctaa cttaaatttt
     1621 ggtagtacaa ggtttaacaa aaatattttg aataaccctt atcaaaattt caacaaccaa
     1681 ttatcatctt ctatttcata tagtaaaaac tggaacagta aatacaattt atctttaaac
     1741 cttaatcata accaaaataa caaaacacat ttagtaaatt taagtttacc aaatgccaat
     1801 tttaatgtag ttacttttta cccttttgaa aaaaaagaag ccattggcac tgctaaatgg
     1861 tatgaaaaaa ttagtattgg atattctgga aatttacaaa acagaatttc attttacgat
     1921 actgccttta gctcctacaa gttgctagat actttgcaat ggggggcaga acataatata
     1981 ccaattggtt tagctttacc tgctttaggc cctattaata ttgcaccaag catttctttt
     2041 caacaaagat ggtttgggca aaaaacacat aggtattgga gcatggcaga ctctttagtt
     2101 aaaacaagta caaacaaagg cttttaccta caaaatagta tgagctttgg cattagtgcc
     2161 agcaccagaa tttttggaac ttaccaattt aaaaaaggta aggtaaaagc aattaggcat
     2221 gaaataagac catcatttgg tattaattat acaccagatt tagtaagcaa aaatttttac
     2281 aatgtacaag tagacacctc tggtagaacc atgcgttttt ctgagtttga tggtggaatt
     2341 tatagctctt acaatgaagg aaattttgga ggtattactt ttggtattga taacttactt
     2401 gaaatgaaag taagagacaa aactgataca agcaaaaatg cggacagaaa aataaaatta
     2461 attgatgggt ttggctttag ctcttcgtac aatttactag cagatagttt tgcattaggt
     2521 agttttaatt tatatgtaag aagtacattg tttgataaaa taaatattac tgcaagtgcc
     2581 agtcttgatc cttatcaagt aaataaattt gggcaaaggg taaatgcatt tatgctaaag
     2641 aaaggaaaat taggaagaat aactaatgga agccttgcta ttagtactaa ctttcaaagc
     2701 aaatctaaag atggcaaaaa tgaagaagat agtaaaaatg gaatacccaa tgacccattt
     2761 ttaacaccag atgaacagca aagacagcta gattacattc gccaaaaccc tgcagaatat
     2821 acagatttta atataccatg gaaggtagat ttatcttact ctctttcatt ttatcaagct
     2881 ataaaaccag attatagtgg ctacaataca gaaactttta gtagcttaaa ttttaatgga
     2941 gattttagtt taagccccaa atggaaagta ggtgcaacag gatattacga tttaaaatac
     3001 atgaattttc aacaatttag catgtttatt tctagagaaa tgcactgctg gcaactttct
     3061 gtaaatatta cccctgttgg tttgtggcgt agttttaata ttgttataag tcctaaaagt
     3121 ggtattctaa gagatttaaa aataaataga aacagaacat tctctaatct gtagtttttt
     3181 tgtttttcca aatcaaacta ccatctccat agcttaaaaa tctaaaatca ttttctaatg
     3241 caaagtggta aatttttttc caattgtttc ctgctattgc agctaccaac aataacaaag
     3301 tagattgtgg ctgatgaaaa ttagtaacta aagcatctgc aacttttaaa ttgtaacctg
     3361 gtgcaataat tatttgtgtt ttagtaataa tcttttcttt attattctca atcatccatt
     3421 gtaataaact atttaaggca tcgtttacca aaatttcttg aggtaactca taagcatccc
     3481 attgattaat ggcaatttca ttaaatgatg taaaattggt ttgtgttttt attttcacac
     3541 ccatccaata aaggctttca attgttctta aagatgttgt accaactgct ataatggtgg
     3601 gaaaatcttt acttaataat tgttcaataa actgcctggt aacttcaata tattctgcat
     3661 gcatttcatg ctcttgcatt gttaaagcct ttacaggttt aaaagtacct gcacctacat
     3721 gtaatgtagt ataaataggt tgaatatttt ttgcctttag gttcttaacc attacatccg
     3781 taaaatgcaa accagcagtt ggggctgcaa cacttccatc aaattttgca taaatagttt
     3841 gataggtaac tctatcttgt tctgtagtgc ttctttttat ataaggtggt aaaggaatta
     3901 cgccaaacaa atgtaaaagt tcagaaaagc atagctcatt attgttccaa caaaattcaa
     3961 tcaaaaaata atcatttttc ttttcaatta tttttgcttg tagttgaata ttatttccat
     4021 cttctttcaa ttcttttagt aaaaaatctt ctttccattt tttggctcca ccaactaaac
     4081 atttccaata tactttactt ttttgcatca tggcaacagt aatatcccca tatctctcat
     4141 ctggttctaa acaaaaaatt tcaattacac tgccagttgt tttttcaaac aataatcttg
     4201 cttcaacaac tttagtgttg ttaaaaataa ttaaagagtt ttcaggaata tgattgtgta
     4261 aattataata atagtcttgt gaaatttctt cattgttata cacaagtagt ttgcttgtat
     4321 ctcttagaga taaaggaaat tttgctattc tttcttcaga aagtggataa gaaaaatcaa
     4381 gaatagataa atgctttgtg tgcattattg aaaatt
//
LOCUS       DAOQHP010000169         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11832_length_4370_cov_3.359907, whole genome shotgun sequence.
ACCESSION   DAOQHP010000169 DAOQHP010000000
VERSION     DAOQHP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11832_length_4370_cov_3.359907"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="PLY81_06290"
     CDS             complement(<1..1926)
                     /locus_tag="PLY81_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNF29871.1"
                     /translation="MKKIHLSIILIVLISFSYAQRGGSLKWNEAGNAYYTTEKGDIVK
                     FSLPKFDKTILVEKKALVPEGKNSELSIKSFSVSTDENVFLIFSNSKKVWRYETRGDY
                     FIYNKNTGKLVQLGKSLSASTLMFAKISPDGKKAAYVSKHNIYVEDLGTNKITKLTKD
                     GTDRIINGTFDWVYEEEFGCRDGFRWNPDSKSIAYWQIDARNIRNFLMINNTDSIYSF
                     TIPVEYPKVGEDPSACKVGVVDIATQKTKWMNVPGSAIQNYITRMEWANNGKLIIQQL
                     DRKQQVSKIYFLNSKTATASLIYTEKDNAWIDIKSRWNNDNPMGWEWVNNNKDFLWVS
                     EKDGWRHIYLISNEGEKETLLTNGEYDMIDFCGIDEKTGFVYFTASPNNALQKYLYKV
                     KLDGTGNAILVSSAEQNGTHSYQVSPNGLYAKHSFSSATIPPSEEWVNLQTGENIKGE
                     IKTKPNKNATTQFFKVTTEDGVTMDGWKILPENFDSTKKYPVVFYVYTEPGSATVRDT
                     YGSASTFLYQGNIAADGYIQISLDGRGTPLPKGAAWRKSIYKKVGILNVRDQAMAAKE
                     ILKWNYVDKSRIAVWGWSGGGSTTLNLLFQYADIYQTGIAIAPVASRLTYDNIYEERY
                     MGLPQENKDDYIQASAIT"
     gene            2128..3003
                     /gene="sucD"
                     /locus_tag="PLY81_06295"
     CDS             2128..3003
                     /gene="sucD"
                     /locus_tag="PLY81_06295"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNF29872.1"
                     /translation="MAVLVNKNSKIIVQGFTGTEGTFHATQMLEYGTNVVGGVTPNKG
                     GTFHIDKPVFNTVAEAVKTTGANVSIIFVPPAFAADAIMEAADAGIALVVCITEGIPV
                     QDMVKVKNYLQGKSTRLIGPNCPGVITAEEAKVGIMPGFIFKKGKIGIVSKSGTLTYE
                     AADQAVKAGLGISTAIGIGGDPIIGTPTVEAVKLLMDDPETEGIIMIGEIGGSMEADA
                     ARWIKDNKTKPVVGFIAGQTAPPGRRMGHAGAIVGGADDTAAAKMKIMAECGIAVVAS
                     PADIGKTMAAELAKK"
     gene            complement(3083..3859)
                     /locus_tag="PLY81_06300"
     CDS             complement(3083..3859)
                     /locus_tag="PLY81_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNF29873.1"
                     /translation="MSFFKSGNPTLSQKMFEKSLNAQAEMQGTMSVRGTMNKFGFLLL
                     TVIGGAAYTWQLYVSGQEKLMTTLMIVGVIGGLISALAIAFKPNWAPFLAPAYGILEG
                     FFIGAISVVINNMLAEEGQPNIIMQAVGLTFGVAIAMFVLYNFRIIKPTEKFKSIIVS
                     ATVGIAIFYGITLLLGFFGVKMDFMAFGNGSLLGIGINLFIVAIAALNLILDFDMIEQ
                     GAERGAPKFMEWYGAFGLLVTIVWLYIEILKLLSRFSGRD"
     gene            4043..>4370
                     /locus_tag="PLY81_06305"
     CDS             4043..>4370
                     /locus_tag="PLY81_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNF29874.1"
                     /translation="MRKIAVAKGDGIGPEIMDATLKIFNAANVALDYQIVEMGKWVFD
                     KGFSNGMTPLAQQTIEQTGILFKGPMETPKGKGVKSVNVTARKTWNTYANKRTFQTLH
                     GVDSVFS"
BASE COUNT         1467 a          777 c          653 g         1473 t
ORIGIN      
        1 tgttatggca gaagcctgaa tatagtcgtc tttattttct tgtggcaacc ccatatagcg
       61 ttcttcatat atattatcat aagttaacct gcttgcaact ggtgcaatag caatgcctgt
      121 ttgatagata tcggcatatt gaaataataa gtttaaagta gttgagccac caccactcca
      181 gccccaaact gcaattctgc ttttatctac ataattccat tttaaaattt cttttgcagc
      241 cattgcttgg tctcttacat tcaaaattcc aactttttta taaatagatt ttctccaagc
      301 agcaccttta ggcaatgggg ttcctcttcc atcaaggcta atctgaatat agccatcagc
      361 agcaatattt ccttgataca aaaaagtact tgcactacca taagtatctc taacagtagc
      421 agagcctggc tcggtatata cataaaacac aacagggtat tttttggtag aatcgaaatt
      481 ttctggtaaa attttccagc catccatagt tacgccatct tctgtggtta ctttaaaaaa
      541 ttgggtagtt gcatttttat ttggctttgt tttaatttct ccttttatat tttctcctgt
      601 ttgcaaatta acccactctt cagatggtgg tatagtggca ctagaaaaac tatgttttgc
      661 atataaccca tttggagaaa cctgataact atgagtgcca ttttgttcag cagaggaaac
      721 taaaattgcg ttacctgttc catctaactt aactttatac aaatattttt gcaatgcatt
      781 gtttggcgat gccgtaaaat atacaaagcc tgttttttca tctataccac aaaagtcaat
      841 catatcatac tctccattag ttaataaggt ttctttttcc ccttcgttgc ttattaaata
      901 aatatgcctc caaccatctt tttcgctaac ccataaaaag tctttattat tattcaccca
      961 ttcccaaccc attggattat cgttattcca cctactttta atatcaatcc atgcattatc
     1021 tttttcagta taaattaaag atgcagttgc agtttttgag tttaaaaaat aaattttact
     1081 tacttgttgt tttctatcaa gttgctgaat tataagttta ccattattgg cccactccat
     1141 tcttgtaata taattttgaa tagcacttcc tggcacattc atccattttg ttttttgggt
     1201 tgcaatatct accacaccta ctttacaagc tgatgggtct tcgccaactt taggatattc
     1261 tacaggaatg gtaaaagaat aaatagagtc ggtattattt atcattaaaa agttgcgaat
     1321 atttcttgca tctatttgcc aatatgcaat gcttttacta tctgggttcc atctaaatcc
     1381 atctctacag ccaaactctt cttcatatac ccaatcaaaa gtgccattaa ttattctatc
     1441 tgtaccatct tttgttagct tagttatttt attagtacct aaatcctcaa cataaatatt
     1501 gtgtttgctt acatacgcag ctttttttcc atcgggagaa attttagcaa acattaatgt
     1561 agatgcactt agtgatttac ccaattgaac taattttcct gtgtttttat tataaataaa
     1621 ataatcccct cttgtttcat atctccaaac ttttttacta ttactaaaaa ttaaaaaaac
     1681 attttcatca gtacttactg aaaagctttt aatacttaat tccgaatttt tcccttcagg
     1741 tactaatgct tttttctcca ctaaaatggt tttatcaaat ttaggtaagc taaattttac
     1801 aatatctcct ttttcggtag tataataagc atttccagcc tcattccatt ttaaactacc
     1861 accacgttgt gcataagaaa aagaaataag aacaattagt atgatgctaa gatgtatttt
     1921 tttcatttct ttaatttttt tgcaaaatat agttttaata gtacaataat aataaaacat
     1981 actttaaaat tataagtggt agttcaactt tacatttact tctattaata aacaatttaa
     2041 aacaactaaa aaagaagggt agtcttttaa attttatatt gccaatgctt tagtttctct
     2101 tatttttgcg acacaaaaaa tcttcaaatg gcagtattag ttaataaaaa ctctaaaatt
     2161 attgttcagg gttttacagg taccgaaggt acttttcatg caactcaaat gttagaatat
     2221 ggaaccaatg ttgttggtgg tgtaacaccc aataaaggcg gaactttcca tatagataaa
     2281 ccagtattta atactgttgc agaagcagta aaaaccacag gagccaatgt tagtatcata
     2341 tttgtaccac cagcttttgc tgctgatgca attatggaag ctgccgatgc aggtattgct
     2401 ttagttgttt gcattaccga gggtattcct gtacaagata tggtaaaagt gaaaaattat
     2461 ttacaaggga aaagtacaag gttaattggt cctaattgcc ctggtgtaat tactgcagaa
     2521 gaagccaaag ttggtattat gcctggcttt atatttaaga aagggaaaat cggcattgtt
     2581 tctaaatctg gtacattaac ttacgaagct gccgatcaag ctgtaaaagc aggtttaggt
     2641 atatctactg ctattggtat tggtggagac ccaataattg gcacaccaac tgttgaagct
     2701 gtaaaacttt taatggatga ccctgaaaca gaaggtatta ttatgattgg cgaaattgga
     2761 ggaagtatgg aagctgatgc tgcacgttgg attaaagaca ataaaacaaa accagtagta
     2821 ggttttattg ctggacaaac agcacctcca ggtcgtagaa tgggtcatgc tggtgcaatt
     2881 gttggtggtg cagatgatac tgctgctgct aaaatgaaaa ttatggcaga atgtggaatt
     2941 gctgttgttg caagccctgc agatattggt aaaacaatgg ctgccgaatt agctaaaaaa
     3001 taaaaaataa gcattaaata aatttaaaag ccccttgttg taatgtacaa ggggctttta
     3061 aatttttacc tgtatttctc ttctaatctc tgccagagaa acggcttaat agttttaaaa
     3121 tttcaatata taaccaaaca atagttacca ataaaccaaa agcaccatac cactccataa
     3181 atttaggggc acctctttcg gctccttgtt ctatcatatc aaaatctaaa attaaattta
     3241 aggcagcaat agcaactata aataggttta taccaatacc taataaacta ccattaccaa
     3301 aggccataaa atccattttt acgccaaaaa agcccaacaa taaggttatg ccataaaaaa
     3361 ttgcaatacc tactgtagca ctaacaataa tacttttaaa tttttcggta ggtttaataa
     3421 ttctgaaatt atacaataca aacattgcaa ttgcaacacc aaaagttaaa ccaacagctt
     3481 gcataataat attaggttgc ccttcttctg caagcatatt attaataaca acagagattg
     3541 cacctataaa aaacccttct aaaataccgt aagcaggagc taaaaaagga gcccaattag
     3601 gtttaaaagc aattgcaaga gctgaaatta agcctccaat tacaccaaca atcataagtg
     3661 tagtcatcaa tttttcctga ccactaacat ataattgcca agtgtaagct gcaccaccaa
     3721 taactgtaag taataaaaag ccaaacttat tcattgttcc cctaacgctc attgtacctt
     3781 gcatttctgc ctgagcatta aggcttttct cgaacatttt ttgagataaa gttgggtttc
     3841 cggatttgaa aaaagacata gttttttaat tttgaactgt aaagataaaa aattgcagat
     3901 taaaatctat caattctctt acaatttaac aatgtataaa aattacctat ttaatatcca
     3961 taataaaatt atttatttac tttttctttc acaagttact acactttcaa cccttacttt
     4021 tgcacctcaa aaataaagta caatgaggaa aatagcagtt gcaaaaggag atggcattgg
     4081 cccagaaatt atggatgcta ctttaaaaat atttaatgca gctaatgttg ctttagacta
     4141 tcaaatagtt gaaatgggta aatgggtttt tgataaaggt tttagcaatg gcatgacacc
     4201 tttagcacaa caaactattg agcaaacagg tattttattt aaaggaccaa tggaaacgcc
     4261 aaaaggaaaa ggtgtaaaaa gtgtaaatgt tacagcacgt aaaacctgga acacttatgc
     4321 aaataaaaga acttttcaaa cattacatgg ggttgattct gtttttagca
//
LOCUS       DAOQHP010000170         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_11993_length_4340_cov_3.640140, whole genome shotgun sequence.
ACCESSION   DAOQHP010000170 DAOQHP010000000
VERSION     DAOQHP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_4340_cov_3.640140"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..764
                     /locus_tag="PLY81_06310"
     CDS             171..764
                     /locus_tag="PLY81_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF29875.1"
                     /translation="MQIDYNILIAYGGVAKKFAKGEYVFIEDAMPNFYYQVVEGEVKV
                     YSSNSNGKEIIQGIFTEGQSFGEPPLLIDKPYPSSAIAITPLVVIRLSKEKLNNILKD
                     FPEISHSFIYTFAERMYNKASIALIRIGTNPEEIIEQFLYKYKEEHKKDNKPMLIPYT
                     RQQIADFTSLRVETVIRTLRKMSNHKIVQIIDHKLYY"
     gene            769..1989
                     /locus_tag="PLY81_06315"
     CDS             769..1989
                     /locus_tag="PLY81_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29876.1"
                     /translation="MNNTYNLKNWFKLGLISLIIVALYGTTMRYKIAFNFPYLEQKNI
                     MHAHSHFGFIGWLSHIIYCGLALNIFPFITKIKQKKYIILIAANLACALGMLISFTLY
                     GYRISSIAFTSLTILINIIFTIAYIFDSKFFPKNNPSKYWAITGLLLNIVSAAGPLSL
                     AYIMTNKVINFNFYLGSVYYYLHFQYNGWFFFGGMAMVSSILPLEFPSIKKYYYAFAI
                     TAIPTFFLSILWAKLPLWLYIITVIATIIQFFAWVMLVYKGYQFLKNKEITTRPKWVN
                     ILFYAAAFALTIKFTLQTISVIPSLSQMVYGFRPIIIAYIHLVLLGVFSLFILGYLFQ
                     KQIFSPSASLKFATLLFLIGVILNELVLAIQGFAALTYTPVPFINEMLFAVAVILLSS
                     SIMLFAFQKKNNKI"
     gene            1991..2383
                     /locus_tag="PLY81_06320"
     CDS             1991..2383
                     /locus_tag="PLY81_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF29877.1"
                     /translation="MQPYFGVEIILYVANQEESKQFYSNLLQLQPILHVPGMTQFTIT
                     ENCSLGLMPNTGIAKIITPAMPHPDQAIGVPKCEVYLYVSNVQFYYEHALAIGAKLVS
                     KLDDRDWGDKACYFADLDGHIIAFAEKL"
     gene            complement(2409..3722)
                     /locus_tag="PLY81_06325"
     CDS             complement(2409..3722)
                     /locus_tag="PLY81_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF29878.1"
                     /translation="MKSKAPLLFIITAIASLITAISFGIIATLQYVQFDFLKPFLTFN
                     KVRPMHVTGALSWVLLSAIGGIYYYLQKDGLLKKQLLVSYHFIIFLLTGIGIYTCYAL
                     GIFGGKEYLEFPPILIIPILIGWFLFAYNVIGSLLNGFTKRPVYFWMWCTGSVFMIYH
                     LSEAYIWLTPYFKDKFLANTALQWKAGGSFVGSWNMLVYGTATYVMAKLSPTSGVGTD
                     KKSFFFYFLGLSNLMFGWAHHVYLIPIAPWIRYFAYLVSMTEWILLISIIYSWKKSLN
                     FNTTIENKVVIKFLSSTDKWVLINLFVALLISIPAINYYTHGTHVTVAHSMGTTIGIN
                     TTILLASIAYIVQKENPNFNFKLVLKGLTFFNIALILFLITLLVAGASRSQWMYALEP
                     SAFAAMQYKLKTVYLIMAILGSLLTTTILVMVLPLFKGLMKALRG"
     gene            complement(3722..4126)
                     /locus_tag="PLY81_06330"
     CDS             complement(3722..4126)
                     /locus_tag="PLY81_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF29879.1"
                     /translation="MSKKFLILIVLICFFGVYNYLVYTQSQPPTKPLLSTNALQGQKL
                     WQDNNCNSCHQFYGLGGYLGPDITNVYSLKGGDFINAMLNSGISTMPLFHFSDLEKQN
                     IITFLKEVDSTGFYPDKKSIIHKNGWVEISEK"
BASE COUNT         1532 a          662 c          607 g         1539 t
ORIGIN      
        1 attggtggtg gcgaatcaac aactatagaa tttaaagcac ctgcaaaagg cacttacgat
       61 ttcttatgtt catttcctgg ccattctgca atgatgaaag gtaaatttat tgtagaataa
      121 gagccgataa ctcagttttt tttgttatta ttatagttta aaactaatta atgcaaatag
      181 actataatat actaatagca tatggtggcg ttgccaaaaa atttgcaaag ggtgaatatg
      241 tttttattga agatgctatg cctaattttt attatcaggt tgtagaaggc gaagtaaaag
      301 tgtattcctc caactccaat ggtaaagaaa ttattcaagg aatttttacc gaagggcaaa
      361 gttttggcga gccaccattg ttaattgata agccttaccc tagctcggca attgccatta
      421 cgccattagt ggtaattaga ctttctaaag aaaaactaaa taatatttta aaggattttc
      481 cagaaatatc acattctttt atttatacct ttgcagaaag aatgtataat aaagccagca
      541 ttgctttaat tagaattggt accaacccag aagaaattat tgagcaattt ttatacaagt
      601 ataaagaaga gcataaaaaa gataataaac caatgctaat accttataca agacaacaaa
      661 ttgcagattt taccagcctt agggttgaga cagttatacg tactttaaga aaaatgagca
      721 accataaaat tgttcaaata atagatcaca aattatatta ttaatataat gaacaacacc
      781 tacaacctaa aaaattggtt taaacttggt ttaattagtt taattattgt tgcattatat
      841 ggtacaacca tgcggtataa aattgctttt aattttccgt atttagagca aaagaatatt
      901 atgcatgccc attctcattt tgggtttatt ggttggcttt ctcatataat ttattgtggt
      961 ttggctttaa atatttttcc atttattact aaaataaaac agaaaaaata tattatactt
     1021 attgctgcaa atttggcttg tgcattaggt atgcttattt cttttacatt atatggttat
     1081 agaatttcat ctattgcatt tactagttta accattttaa taaatattat ttttactata
     1141 gcatatattt tcgatagtaa attttttcct aaaaataatc cttcaaaata ttgggcaatt
     1201 acaggtttat tattaaacat tgtttcggct gcaggcccat taagtttggc atatataatg
     1261 accaataagg ttattaattt taatttttat ttaggctcgg tatattatta tttacatttt
     1321 caatataatg gctggttttt ctttggtggt atggcaatgg tttctagtat tttaccttta
     1381 gagtttccat ccataaaaaa atattattat gcttttgcca ttactgcaat acctactttc
     1441 ttcttatcaa ttttatgggc taagttgcct ttatggcttt atattataac agttattgct
     1501 actataattc aattttttgc gtgggtaatg cttgtatata agggctatca gtttcttaaa
     1561 aataaagaaa taactacaag acctaaatgg gttaatattt tattttatgc tgcagctttt
     1621 gctttaacaa taaaatttac acttcaaacc atttcagtta ttccttcact aagccaaatg
     1681 gtttatggtt ttaggcctat tattattgcc tacatacact tagtattatt aggtgtattt
     1741 agtttattta ttttaggtta tttatttcaa aaacaaattt tcagcccttc ggcttcttta
     1801 aaatttgcta cactactttt tttaattggt gttattttaa acgaacttgt attagctata
     1861 caaggctttg cagcacttac ctatacacct gtgcctttta ttaatgaaat gctttttgct
     1921 gttgcagtaa ttttactttc tagttctatt atgttatttg catttcaaaa aaaaaataac
     1981 aaaatatagt atgcaacctt attttggtgt agaaataatt ttatatgtag ctaatcaaga
     2041 agaaagtaaa caattttata gcaacctcct tcaattacaa cctatacttc atgtgccagg
     2101 aatgacccaa tttacaatta ctgaaaattg ttcacttggt ttaatgccca atactggaat
     2161 tgcaaaaatt attacgccag caatgcctca tcctgatcaa gcaattggtg taccaaaatg
     2221 cgaagtatat ttatatgtaa gtaacgttca attttattat gaacatgctc ttgcaatagg
     2281 agcaaaactt gtaagcaagt tagatgacag agactgggga gataaggcct gttattttgc
     2341 agatttagat gggcacatta ttgcttttgc tgaaaaatta taaccaccca tcctcaacaa
     2401 ataaatttct atcctcttag tgccttcatt aatcctttaa ataatggcag caccattact
     2461 aaaattgtag tagttaataa tgaacctaaa attgccatta ttaaataaac agtttttagt
     2521 ttgtattgca tggcagcaaa tgcagatggc tctaaagcgt acatccattg gcttcttgat
     2581 gctcctgcaa ctaaaagtgt aattaaaaat agaataaggg caatattaaa aaaggttaag
     2641 ccttttaaaa ctaatttaaa attaaaattg gggttttctt tttgtacaat ataagcaata
     2701 gatgctaata atatagtggt attaattcca attgttgtgc ccatactatg agcaacagtt
     2761 acatgtgtac catgcgtata ataatttata gcaggtatag aaattaacag agcaacaaat
     2821 aaattaatta gtacccattt atctgtagag gataagaact taattacaac tttgttttcg
     2881 atggttgtat taaaattcag cgatttcttc caagagtaaa taatgctgat aagcaatatc
     2941 cattcagtca tacttactaa atatgcaaaa tatcttatcc aaggtgcaat aggaattaag
     3001 taaacatgat gtgcccagcc aaacataaga ttggataagc ctaagaaata aaagaaaaaa
     3061 gatttcttat cagtaccaac gccacttgtt ggactaagtt ttgccataac ataagttgca
     3121 gtaccataca ctaacatatt ccaagaacct acaaatgatc ctccagcctt ccattgtaaa
     3181 gctgtattag ctaaaaattt atctttaaaa tatggtgtta accaaatata agcttccgat
     3241 aaatgataaa tcataaatac cgaacctgta caccacatcc aaaaataaac tggtcgttta
     3301 gtaaacccat ttaataaaga ccctattaca ttgtatgcaa acaaaaacca accaattaaa
     3361 ataggaatga tgagtattgg aggaaactct aaatattctt tacctccaaa aattcccaaa
     3421 gcataacagg tataaattcc aattccagta agtagaaaaa taataaaatg ataactaact
     3481 aaaagttgtt tttttaatag cccatctttt tgtaaatagt aataaattcc cccaatagca
     3541 gaaagtaaaa cccaacttaa tgcaccagtt acatgcatgg gtctaacttt gttgaatgtt
     3601 aaaaaaggtt ttaaaaaatc aaactgaaca tattgtaatg ttgcaataat tccgaatgaa
     3661 atagcagtta ttaaagatgc tattgctgta atgataaata ataaaggtgc ttttgatttc
     3721 attatttttc tgatatttct acccaaccgt ttttatgaat aatacttttt ttatcaggat
     3781 aaaatcctgt gctatctact tcttttaaga atgtaataat attttgtttt tccaggtctg
     3841 aaaagtggaa taatggcatt gtacttattc cgctatttaa cattgcattg ataaaatctc
     3901 ctccttttaa agaatataca tttgtaatat ctggtcctaa ataaccacct aacccataaa
     3961 attgatgaca ggaattgcag ttattatctt gccacaattt ttgcccttgt aaagcatttg
     4021 tagatagcaa tggctttgtt ggaggctgac tttgagtata aaccaaataa ttatatactc
     4081 caaaaaaaca aattaataca attaaaatta gaaatttttt agacattttt tgaatcaaaa
     4141 attatggaca aatttaattt ttcaatgtgc atttaatcat gacctgtatc ataagaaaac
     4201 tataacttat aatgaacaac aactaaattt tgctgagcat tacactagat atagacttag
     4261 catgcatttt cattttttct gcattctttc cttcaaaaaa ctcatccaca gttgcattaa
     4321 ataatttcat ccatctttca
//
LOCUS       DAOQHP010000171         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12009_length_4337_cov_2.978982, whole genome shotgun sequence.
ACCESSION   DAOQHP010000171 DAOQHP010000000
VERSION     DAOQHP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_4337_cov_2.978982"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLY81_06335"
     CDS             complement(<1..943)
                     /locus_tag="PLY81_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF29880.1"
                     /translation="MLKKHLLQFVLALFMLPAIVVAQNTTSTLSGSVKTTTGEALIGA
                     TVIATHEPTGTVYKVQARSGGRYDISNMNPGGPYSIVVSFVNFQNEKKSDIYLGLGET
                     YKIDFILVAKATDLGEVTVKTTSKKTTDFTSKGGAETFITKEKMDNLPTVGRNLQDYL
                     RFVPQAKVASSDGNLAGVSFGGQNNRYNSFYVDGAANNDQFGLASSGTNGGQTGSSPI
                     SIDAIDQIQIILSPYDASIGNFTGAGINATTKSGTNKLQASVYTFFRNQDLTGKTPNG
                     DKKLATKLAKFSSKTYGFRVGGPIVKNKAFFFLNVDLV"
     gene            1166..2365
                     /locus_tag="PLY81_06340"
     CDS             1166..2365
                     /locus_tag="PLY81_06340"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNF29881.1"
                     /translation="MLHTIAEAIEDIKNGKMIIVVDDEDRENEGDFIIAAHYATPEVI
                     NFMSKEGRGLICVALTEQRCLELDLQPMVNSNTSLHETAFTISVDLVSNETTTGISAQ
                     DRSKTIQALINSATKASDLGRPGHIFPLKAKNGGVLRRAGHTEATVDIARLAGLEPAG
                     VLVEIMNEDGTMARLPQLVEISKKLNLKIISIKDLIEYRLNKDSLIEELVSVDMPTKY
                     GHFNLVAFKEKLSGAEHLALIKGSWQKDEPVLTRVHSSCFTGDILGSFRCDCGEQLHS
                     AMQMVDKAGKGVILYMNQEGRGIGLINKLKAYNLQENGMDTVEANLHLGFKMDERDYG
                     VGAQILRHLNVTKLKLLSNNPRKRAGLAGYGLEIVEVLPIEVTPNTHNEKYLTTKRDK
                     MGHTILK"
     gene            2445..3008
                     /locus_tag="PLY81_06345"
     CDS             2445..3008
                     /locus_tag="PLY81_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29882.1"
                     /translation="MHTIEPYYNWRHIYISEEDKRSPFYKRFYSEFEYTQTVYNYFIH
                     PQWDFFGSKTLYLKIIYVDYELNFAIIELIGEWNDAIENDIMELKREVIDKLMEEGIF
                     KFILIAENVLNFHSGDKEYYEEWFEQVTDSNGWIVALNMPDATQYDFKKRKLNYFIEL
                     QELENWRVYKPYHLFKKIEVELNNRLT"
     gene            complement(3027..4073)
                     /locus_tag="PLY81_06350"
     CDS             complement(3027..4073)
                     /locus_tag="PLY81_06350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNF29883.1"
                     /translation="MLVRLVMLCFLFSLTNFSKAQFNADAVNPVLMQNKKQLGNEAVL
                     MVYKDGKVIYKKKTDDYYDERAQTPIGSASSWLTAVLIMQYIENGTITLDTKIADYIP
                     IYIPYLKKYITIGHCLAQLTGIDFKKGASSSKEASKFNSLEEMVNSYAKKEIRANTGS
                     DFWYAEMHINIAARVIEVITKKKFEQLISKNVIKPLGMKGTSFSPSGGGCPNPAAGAV
                     STANDFANFLSMIMNKGMFNGKQILKEETINTMLTVTYTPSMVTYMPKAAENFEYGLG
                     AWILEKDINNKPAVFGTTNFEGIWCVIDKCRNYGMILFTKKELSDARKDVFYTLKNVI
                     DEQLPPTNGTSCNN"
BASE COUNT         1532 a          625 c          688 g         1492 t
ORIGIN      
        1 taactaaatc aacatttaaa aagaagaaag ctttattttt tacaattggg cctccaactc
       61 taaaaccata agttttgcta gaaaacttgg ctaatttagt tgcaagtttt ttatcgccat
      121 taggtgtttt acctgttaaa tcttggttgc ggaagaaagt ataaacagaa gcttgcaatt
      181 tattagtacc acttttagtt gtagcattaa taccagcacc agtaaaatta ccaatagagg
      241 catcgtaagg agaaaggata atttgaattt gatctattgc atcaattgaa attggtgaag
      301 aacctgtttg gccaccatta gtaccagatg aagctaaacc aaattggtcg ttattagcag
      361 caccatccac ataaaaagag ttataacgat tgttttgccc accaaatgaa actccagcta
      421 agttaccatc agagcttgcc acttttgctt gtggtacaaa acgtaaatag tcttgtaagt
      481 ttctaccaac tgttggtaag ttatccattt tttctttagt aataaaagtt tcggcacctc
      541 ctttactagt aaaatcagtt gtttttttag aagttgtttt aacagttact tctcctaaat
      601 cagtagcttt tgcaactaaa ataaaatcta tcttgtaagt ttcacctaag ccaagataaa
      661 tatcactttt tttctcattt tgaaaattca caaaagaaac aacaatacta taagggcctc
      721 cagggttcat gttactgatg tcgtaacgac caccagaacg agcctgtact ttatatacag
      781 ttccagttgg ttcatgcgtt gcaataactg tagcaccaat taatgcctct ccagtagtgg
      841 tttttaccga accacttagt gtactggttg tgttttgtgc tacaacaatt gctggcaaca
      901 taaacaatgc caatacaaat tgtagtaaat gttttttaag cataattttg atatttgttt
      961 ttgcaaagaa agtgaataaa gtataataat ctgtaacaat gcgttaacaa atttttattt
     1021 tttttatgtt tttgtgcata aactgtgcaa ttttttttct tttttttaat ttttagcatg
     1081 tttaagtaaa aaatttagat gcattttagc caaacaaggt ttaggcgtac atttgcattc
     1141 ttttaaattg atttaaggtt aaaacatgtt acatactatt gccgaagcca tagaagatat
     1201 taaaaatggc aaaatgatta ttgttgtgga tgatgaagac agagaaaatg aaggggattt
     1261 tattattgct gcacactatg ctacaccaga agtgattaat tttatgagca aagagggtag
     1321 aggcttaatt tgtgttgccc taacagagca aaggtgtttg gaacttgatt tacagccaat
     1381 ggttaattct aatacctctt tacacgaaac tgcattcact atttcggtag atttagtaag
     1441 taacgaaact actactggca tttctgctca agatcgttca aaaactatac aggctttaat
     1501 taattcagca acaaaagcat cagatttagg aagacctggg catatatttc ctttaaaagc
     1561 aaaaaatgga ggtgttttaa gaagagcagg gcatacagaa gctactgtag atattgcaag
     1621 gcttgctggt ttagaacctg caggagtatt agtagaaatt atgaatgaag atggtacaat
     1681 ggcaaggttg ccacaacttg tagaaatttc aaagaaatta aatcttaaaa ttatttctat
     1741 aaaagattta attgaatata gactgaataa ggatagttta attgaagaat tagttagtgt
     1801 agatatgcct actaaatacg gccattttaa tttagttgca tttaaagaaa aattatctgg
     1861 agccgaacat ttagcattaa taaaaggttc ttggcaaaaa gatgaacctg tattaaccag
     1921 ggtgcatagt agttgcttta caggagatat tttaggcagc tttagatgcg attgtggcga
     1981 acaacttcat tctgcaatgc aaatggtaga taaggcaggt aaaggagtaa ttctttatat
     2041 gaatcaagaa ggaagaggaa ttggtttaat aaataaatta aaggcctata accttcaaga
     2101 aaatggaatg gataccgttg aagctaattt acatttaggg tttaaaatgg atgaaagaga
     2161 ctatggcgtt ggagctcaaa ttttacgcca tcttaatgta acaaaactta agttactgag
     2221 caacaatcca cgtaaaagag ctggccttgc aggttatgga ttagagattg tagaagtatt
     2281 accaatagaa gttacaccaa atacacataa cgaaaagtat ttaacaacca aaagagataa
     2341 aatgggtcat acaattttaa aataactcgt tttatttagt aagttttgta caacaatttt
     2401 ccagcttttt ctaatcattt atttgatgca attttgcaat aacaatgcat acaattgaac
     2461 catattataa ttggaggcat atttatattt cggaagaaga taaaagaagc cctttttata
     2521 aaagatttta cagcgagttt gaatataccc aaaccgttta taattatttt atacatcctc
     2581 aatgggactt ttttggtagc aaaactttat accttaaaat tatttatgta gattatgaat
     2641 tgaattttgc cataattgaa ttaattggcg aatggaatga tgctatagaa aatgatatta
     2701 tggaattaaa acgtgaggta attgataaac taatggaaga gggtattttt aaatttattt
     2761 taattgcaga aaatgtatta aactttcata gtggagacaa agaatattac gaagaatggt
     2821 ttgaacaagt tacagattct aatggttgga ttgtagcttt aaatatgcca gatgcaaccc
     2881 aatatgattt taaaaaaaga aaattaaatt attttattga gttgcaagag ttagaaaatt
     2941 ggcgtgtgta caagccctat catttattta aaaaaataga ggtggaatta aataacagat
     3001 taacttaatg caaaaaatat ttttaattaa ttattacagc ttgtaccatt tgtaggaggc
     3061 aattgctcat caattacatt ttttaaggta taaaaaacat cttttctagc atcagataat
     3121 tctttttttg taaataatat cataccataa tttctacatt tatctattac acaccatata
     3181 ccttcaaaat tagtagtacc aaaaactgca ggcttattat tgatatcttt ttccaatatc
     3241 catgcaccta atccatattc aaaattttct gcagctttag gcatataggt aaccatactt
     3301 ggtgtataag taacagtaag catggtatta atagtttctt cttttagtat ttgtttacca
     3361 ttaaacatgc ccttattcat tatcatactt aaaaaattgg caaaatcatt tgctgtactt
     3421 acagcacctg cagcagggtt agggcagcca ccaccagatg gagaaaagct agttcctttc
     3481 atgcctaatg gcttaatcac atttttagaa ataagttgtt caaatttttt cttagtaata
     3541 acttcaatta ctcttgcagc aatatttata tgcatttctg cataccaaaa atcggatcct
     3601 gtatttgctc taatctcttt tttagcatag ctgttaacca tttcttctaa actattaaat
     3661 ttagaagcct ctttagaaga gcttgcacct tttttaaaat caattcctgt taattgtgct
     3721 aaacaatgcc caatagtgat gtattttttt agatagggta tataaatagg aatataatcg
     3781 gcaattttag tatctaatgt tatggtgcca ttttcaatat attgcattat taaaacagca
     3841 gttagccacg agcttgcact accaattggg gtttgtgctc tttcatcata ataatcatcc
     3901 gttttctttt tataaattac tttaccatct ttataaacca ttaaaacagc ttcgttgcca
     3961 agttgttttt tattttgcat taatactggg tttacagcat ctgcattaaa ttgtgcttta
     4021 gaaaaattag ttagagaaaa taggaaacat aacattacta aacgaacaag cataataaaa
     4081 aatttaaaaa caatattaat aaatccgaat tgtttgagta gaattatttt ataaacaaac
     4141 ttttttaata aaattaaatt aatattataa ccataatcaa gctaaccata aactgtaatt
     4201 atttcataaa attcaaactg agtaagactt tcaggcagta ttgataataa actactgaca
     4261 cattttctta tttactatac tggtttttta tttgtagtac ttctataata aattattaaa
     4321 aaatatgaat ttaaata
//
LOCUS       DAOQHP010000172         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12032_length_4332_cov_3.346505, whole genome shotgun sequence.
ACCESSION   DAOQHP010000172 DAOQHP010000000
VERSION     DAOQHP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12032_length_4332_cov_3.346505"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLY81_06355"
     CDS             <1..1011
                     /locus_tag="PLY81_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNF29884.1"
                     /translation="LGGKKRNAFTISVFDTKFSNAYDPLTGTYTSNAANKSYIKTSGA
                     SVSLAKQLKWPDDYFSLSIGLSFSRYKLKNYYIDQVNLPGFNNGFSNNLSLKFALQRS
                     SVDNPLFPRQGSNFLASLQLTPPYSLFDKTIVTSVNPYQWVEYHKWRFNSEWYVPLTK
                     AVGPEKNKQLVLKAAAKFGFIGKYNNKLKVSPFERFQLGDAGLSNQFALLGYDIIAQR
                     GYPVYQTSNPKVNPDQQGASEYFTIFNKYVLELRYPLSLNPSSTIYALGFFEAANGWF
                     SMKDYNPFQLRRSVGIGMRFYLPMFGLLGFDYGIGLDRITPNGKLKDAAKFTFMLGFE
                     PE"
     gene            1043..1555
                     /locus_tag="PLY81_06360"
     CDS             1043..1555
                     /locus_tag="PLY81_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF29885.1"
                     /translation="MKKIILLLVCTLATTIFINAQRYAIIDTKYILDKIPEYKNADRK
                     LQSIAEQWQKEIDNKQAELDKMYKDYEAEQFMLSDDLKKKREDEIFNKEKEVRELQKK
                     RFGYEGDLFKERQKLVKPIQDRVYTAVQKIAVNRGYDFILDKSEGITVIFADPKIDKS
                     EDILKELGIK"
     gene            1590..2189
                     /locus_tag="PLY81_06365"
     CDS             1590..2189
                     /locus_tag="PLY81_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF29886.1"
                     /translation="MKKVFMACAALVISLAATTKSNAQQIKIGMFDEEAVLQFMPGIS
                     KVDTLLHQFVEDSLQNEYEDELYLYQTKDSTFKADSSKMSNSLKLTLKKEIALHFNKL
                     QNWQQYQQQRLQIKQQELLRPYLEKIYGALQAIIIEQKYTHIFKKDVFVHIEKGEELM
                     LRVLHKLKVPVPKEIEEQYKSFGIGGASATKPTTTPKKN"
     gene            2335..3168
                     /gene="murI"
                     /locus_tag="PLY81_06370"
     CDS             2335..3168
                     /gene="murI"
                     /locus_tag="PLY81_06370"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNF29887.1"
                     /translation="MHKGAIGIFDSGYGGLTILKDIEQKLPEYSFIYLGDNARAPYGS
                     RSFETVYQYTWQAVQWFFSQGCHLVVIACNTASAKALRNIQQNNLPSLSNNLKRVLGV
                     IRPTAEVIGNYTNTNNIGILGTTGTIQSKSYILEIEKFFPSIKVYQQACPMWVPLIEN
                     NEYNSKGADYFVKKYINQLTEQSADIDSILLACTHYPLLINKIREYAPADMAIVTQGE
                     IVATSLSQYLNRHQQLDAICKKEGSTNFFTTDDPLMFNKQASIFYGKAVESNYVSLHL
                     L"
     gene            complement(3282..>4332)
                     /locus_tag="PLY81_06375"
     CDS             complement(3282..>4332)
                     /locus_tag="PLY81_06375"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29888.1"
                     /translation="YLKTLNVSNHWTLNSAITASATGAFKNTDLTDSIFPKTGTRFYV
                     QDNYSGANSTGHSSVLYAGCFNFSSPANISFWMTQDNSLNTSLDSIYVVVSTDKAATW
                     TRLQGFGRLSASALLPTWVQQTVSLNSYAGQTIHIGLEGVSKWGNVIGVDDISVTANA
                     PVPVTFGEFVGVRENNSNQLKWNTYTETNNQGFELQRSVNGTEFTTIAFVPTKAINGN
                     SNSELNYTFQDKASSATTYYQLKQLDKDGKYAYSKVVSIKGEKPTRFELANLYPNPAK
                     ETIKVTVAAPKAAVVTLTVTDMTGKVVMKQNNQLTMGDNNIALNVAALNAGNYIVRLS
                     CADGCEYSIQRFVKQ"
BASE COUNT         1598 a          657 c          667 g         1410 t
ORIGIN      
        1 ctgggtggca aaaaaagaaa tgcttttacc atttccgttt ttgatactaa attttctaat
       61 gcctacgacc ctttaacagg cacctataca agcaatgcag ccaataaatc gtatatcaaa
      121 acatcgggtg catcggtaag tttagcaaaa caattaaaat ggcccgatga ttatttttca
      181 ttaagcattg ggttaagttt ttcaaggtat aaacttaaaa attattatat agatcaggta
      241 aatttaccag gctttaataa tggcttttct aataatttaa gtttaaagtt tgctttacaa
      301 cgttcctctg tggataatcc tttatttcca agacagggtt ctaacttttt agcaagctta
      361 caattaacac ctccttattc tttatttgac aaaacaattg ttacctctgt taacccttac
      421 caatgggttg agtatcataa atggcgtttc aattcagaat ggtatgtacc attaaccaaa
      481 gctgtaggac cagaaaaaaa caaacaatta gttttaaaag ccgctgccaa atttggtttt
      541 ataggtaaat acaataataa attaaaggtt tctccatttg aacgttttca attaggcgat
      601 gctggtctat ctaatcaatt tgcattactt ggttatgata ttattgcaca aagaggatat
      661 cctgtgtacc aaacttctaa tccaaaagta aatccagatc aacaaggagc ttctgaatac
      721 tttaccattt ttaacaaata tgtgttagag ctaagatacc ctttaagttt aaacccaagt
      781 agcactattt atgcattagg cttttttgaa gctgcaaatg gctggtttag tatgaaagat
      841 tataatccat ttcaacttcg acgttctgtg ggtataggta tgcggtttta tttaccaatg
      901 tttggcttgc ttggttttga ttatggcata ggattggata gaataacacc aaatggcaaa
      961 ttaaaagatg ccgctaagtt tacattcatg ttaggctttg agcctgaata aaaattaatt
     1021 tttcaagtaa aaaaataaac tcatgaaaaa aattattcta ttattggttt gcactttagc
     1081 aaccaccatt tttattaatg cacagcgtta tgcaattatt gatacaaaat atattttaga
     1141 taaaatacca gaatataaaa atgctgatag aaaattacaa tctattgcag agcaatggca
     1201 aaaagaaatt gacaacaaac aagcagagtt agataaaatg tataaagatt atgaagcaga
     1261 acagtttatg cttagcgatg atttaaagaa aaaaagagaa gatgaaattt ttaacaaaga
     1321 aaaagaagta agagaattac aaaaaaaacg ctttggatat gaaggagatt tgtttaaaga
     1381 aagacaaaaa ttagtaaaac caatacaaga cagagtttat acagcagtac aaaaaattgc
     1441 cgttaaccgt ggctacgatt ttatattaga caaaagtgag ggaattactg ttatatttgc
     1501 cgaccctaaa attgataaaa gcgaagacat acttaaagaa cttggaatta aataaaccct
     1561 atttattaaa taaaaaataa acaaaaagga tgaagaaagt ttttatggct tgtgctgctt
     1621 tagttattag ccttgcagca acaacaaaat caaatgccca acaaattaaa ataggcatgt
     1681 ttgatgaaga agcagtatta caatttatgc ctggcatttc taaagtagat actttattac
     1741 atcaatttgt agaagactct ttacaaaatg agtatgaaga tgaattgtat ttgtaccaaa
     1801 caaaagatag tacttttaaa gcagactctt ctaaaatgag caactcttta aagttaactt
     1861 tgaaaaaaga aattgcctta cattttaata aattacaaaa ttggcagcaa taccaacaac
     1921 aaagattaca aattaaacaa caagagttat taaggccata tttagaaaaa atttatggtg
     1981 cattacaagc tatcattatt gaacaaaaat atactcatat tttcaagaag gatgtttttg
     2041 tacatataga aaaaggagag gaattaatgc tacgtgtatt acacaaatta aaagtgccag
     2101 ttccaaaaga aattgaagag caatataaat catttggtat tggtggagca tctgcaacaa
     2161 aaccaaccac tactccaaag aagaattaaa tcattttgta gattaacata ttttctaaag
     2221 ctacctccta aaaaggtagc ttttttgtac cctttttctt ctgtaagtat taataaaaat
     2281 gcagaatttt agattttaac tttcttcaat taactttaag cactaaataa aaatatgcat
     2341 aaaggagcaa taggaatatt tgatagtggt tacggtggtt taacaatttt aaaagatata
     2401 gagcaaaaat tgcccgaata tagttttata tacttaggcg ataatgccag agcaccttat
     2461 ggttctcgta gttttgaaac agtttatcaa tatacttggc aggctgtaca atggtttttt
     2521 agccaaggct gtcatttagt tgttatagct tgtaatacag cttctgcaaa agctttaaga
     2581 aatatacaac aaaacaattt acccagctta agcaataatt taaaaagagt attaggcgtt
     2641 attaggccta cggcagaagt aataggtaac tataccaaca ctaataatat tggtatatta
     2701 ggtactacag gcactataca atctaaatct tatattttag aaattgaaaa atttttccct
     2761 tctataaaag tttaccagca agcttgccca atgtgggttc ctctaattga aaataatgaa
     2821 tacaatagta aaggggctga ttattttgta aaaaaatata ttaaccagtt aacagaacaa
     2881 tctgccgata ttgactccat attattagct tgtacacatt accctttgct aataaataaa
     2941 ataagagaat atgccccagc cgatatggct attgttaccc aaggtgaaat tgttgctact
     3001 agtttaagcc agtacttaaa taggcatcaa caattagatg ctatttgtaa aaaagagggc
     3061 tccactaatt tttttacaac agatgatcca ttaatgttca acaaacaagc aagcatattt
     3121 tatggtaagg ctgtagagag caactatgtt agcttacatt tattataaaa taatatctta
     3181 tttaaagtaa aatataaatt ttaagaacat ttaaaaataa taacaaaaaa agatgctgct
     3241 tgttttcaag cagcatcttt aatatattct ttataaaact tttattgttt tacaaatctt
     3301 tgaatagagt attcacaacc atcagcacaa gataatctaa caatataatt tcctgcattt
     3361 aaagcagcaa catttaaagc tatattatta tcacccatag ttaattgatt attttgtttc
     3421 ataaccactt taccagtcat atctgtaaca gttaaagtta ctacagcagc tttaggtgca
     3481 gcaacagtaa cttttatagt ttctttagct gggttaggat acaaattagc taattcaaat
     3541 ctggttggtt tttcgccttt aatagaaact actttagaat aagcatattt gccatcttta
     3601 tctaattgtt ttaattgata gtaagtagta gcactgctag ctttatcttg gaaagtataa
     3661 tttaattcag aattactatt accattaatt gctttagttg gaacgaaagc aatagttgta
     3721 aactctgtac cattaacact acgttgtaat tcaaatcctt ggttattagt ttccgtataa
     3781 gtattccatt ttaattgatt actgttattt tctcttacac caacaaattc gccaaatgtt
     3841 acaggtacag gtgcattagc agtaacagaa atatcatcta cacctataac attaccccat
     3901 ttgcttacac cttctaaacc aatatgtata gtttggccag cataagaatt taagcttaca
     3961 gtttgttgaa cccatgttgg taataatgca ctagcactaa gcctaccaaa accttgtaat
     4021 cttgtccatg tagcagcttt gtctgtagat acaacaacat aaatactatc taaagatgta
     4081 ttaaggctat tatcttgagt catccagaag gagatgtttg caggagaaga gaagttgaag
     4141 cagcctgcat ataaaacact actgtgccct gtactatttg caccagagta gttatcttga
     4201 acataaaatc tagtaccagt tttagggaag atagagtcgg ttaaatcggt atttttaaaa
     4261 gctcctgttg cactagcagt tattgcagag tttaatgtcc agtgattact aacatttagt
     4321 gtttttaaat at
//
LOCUS       DAOQHP010000173         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12040_length_4329_cov_5.006083, whole genome shotgun sequence.
ACCESSION   DAOQHP010000173 DAOQHP010000000
VERSION     DAOQHP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_4329_cov_5.006083"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="PLY81_06380"
     CDS             <1..1403
                     /locus_tag="PLY81_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNF29889.1"
                     /translation="VLTFTIDPVDAKDFDDAISVKKINDELYEIGVHIADVSYYVKPD
                     TALDEEAYKRATSVYLPDRVNPMLPEKISNELCSLRPNEDKFTFSAVFEISTNGNVKN
                     YWLGKTVIHSNHRFTYEDVQDIINGKIGEYESEILLLHKIAQIIRTNRFKKGAINFSS
                     QEVRFKLDENAKPISIIVKESKASHQLIEEFMLLANKTVAEHIDKSSNNNPIPFPYRV
                     HDQPDEEKLLPFIEFAKKFGHTFNISSPEKIAESFNTLLENVKGKPEQQVLEQLGIRT
                     MAKAVYTTKNIGHYGLAIPHYCHFTSPIRRYPDVLVHRILYSFLQGKTIMDKKMEEKC
                     RHCSEREKAAMDCERAGNSYKQVEFLKEHLGEDFEGVISGVASFGFWVETLAHKCEGM
                     VSIVSLSDYDDFKFVETDFCLVGKRSGKTFRIGDKVTIKVVAANLEKRKLDYEWVTND
                     NMEATKPVKKKIKKQK"
     gene            complement(1405..1926)
                     /gene="idi"
                     /locus_tag="PLY81_06385"
     CDS             complement(1405..1926)
                     /gene="idi"
                     /locus_tag="PLY81_06385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNF29890.1"
                     /translation="MEKVILVDEYDNEIGIEEKIKTHQAGLLHRAFSVFIVNKNGDLL
                     LQQRSLNKYHSAGLWTNTCCSHPRPNENVTNAAERRLQEEMGFTTKLKPIFQFIYNVA
                     FENGLSEHEYDHVLLGEYNGEISINEQEVADFKFVALDAIEDLIATTPEQFTTWFRIA
                     FPRLKQWLQANSH"
     gene            complement(1969..2262)
                     /locus_tag="PLY81_06390"
     CDS             complement(1969..2262)
                     /locus_tag="PLY81_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF29891.1"
                     /translation="MFKDLDPILHSQLRLAIISLLIGVKEADFNFIKEKTKSTAGNLS
                     VQIQKLKDAGYIEVTKQFKDNYPLTTCSITKQGVHAFEIYVKALQGYLHTDKN"
     gene            complement(2255..2941)
                     /locus_tag="PLY81_06395"
     CDS             complement(2255..2941)
                     /locus_tag="PLY81_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29892.1"
                     /translation="MKSNKPLTEKESLDLITSMIQNAKASYHETGTNAILWGCVIAIA
                     SLLTYLSIELNFKLSFSPFLLTIIAIAFQIYISIKAAKESKVTKFEDAAINAVWLAFG
                     ISIMGLVLYLNIIPNSSIKLATQEGWQFTKHYLDANKPDEPLTPFAPSSYSLFILLYA
                     LPTFTTGIVKNYKPMIVGAILTYVLFIISCYTSTKYDMLAGTIAAISCWLIPGLILRR
                     NYLQQKNSNV"
     gene            complement(2931..3521)
                     /locus_tag="PLY81_06400"
     CDS             complement(2931..3521)
                     /locus_tag="PLY81_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29893.1"
                     /translation="MEKELTPTESLAIIENMINKAKSNYSDDSTLYLLWGWVIFICSI
                     GHFILLQTNIIKYPEYIWVLTWGAVIYQIYYILKKEKKKKVKSYTDEIVGYVWMGFGF
                     TMFFISIIIGNQNNWITLYPIILFMYGTPTFISGVAIRFLPLKIGGIVCWGLAFIAKF
                     TADIYVLLLLALAVLAAWIVPGYLLKAKYKNNSYEE"
     gene            complement(3553..4185)
                     /locus_tag="PLY81_06405"
     CDS             complement(3553..4185)
                     /locus_tag="PLY81_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29894.1"
                     /translation="MKKIILCLTLFISTSIIAQSDKFNAAMSSSIKEYEAAKTAEENI
                     AIAQKFIRIAEAEKTEWLPYYYAATIKAKMSIMGIGGDKDEVADEAQSLIDKAEALSK
                     NNCEILVIKSMIATAKMIVDPQNRYMEFGTLINKYLAEAKKADANNPRPYILESSNLV
                     NTPEQYGGGCNAAKPIAQKAMELFNSFKPASEIHPNWGKEMVEQILKNCK"
BASE COUNT         1501 a          660 c          660 g         1508 t
ORIGIN      
        1 aagtattaac ttttactatt gacccagtag atgcaaaaga ttttgacgat gctatttctg
       61 taaaaaaaat aaatgacgaa ttgtatgaaa taggtgtaca tattgccgat gttagttatt
      121 atgtaaagcc agatacagct ttagatgaag aagcatacaa aagagccaca tctgtttatt
      181 tgccagatag agttaatcca atgttgcccg aaaaaatatc caacgaacta tgttctttac
      241 gccccaacga agataagttt accttttctg ctgtgtttga aatatctacc aacggaaatg
      301 taaaaaatta ttggttgggc aaaacggtta ttcattcaaa ccacagattt acttatgaag
      361 atgtacaaga tattataaat ggcaaaattg gtgagtatga atctgaaata ttattactgc
      421 ataaaatagc acaaattatt agaaccaata ggttcaagaa aggtgctata aatttttcat
      481 cgcaggaagt aagatttaag ttagacgaaa atgctaaacc aataagtatt attgtaaaag
      541 aaagcaaggc atcgcatcag ttaattgaag agtttatgtt acttgccaat aaaactgttg
      601 cagaacatat tgataaatcc agtaacaata acccaatacc ttttccttat agagtacatg
      661 accaaccaga cgaagaaaag cttttaccct ttatagaatt tgccaaaaaa ttcggtcata
      721 catttaatat ttcatcaccc gagaaaattg cagaaagctt taatactctt ttagaaaatg
      781 taaaaggcaa gccagagcaa caagtgttag aacaattagg aataagaaca atggcaaaag
      841 cagtttatac aacaaaaaac attggccatt atggtttagc cattcctcac tattgtcatt
      901 ttacttctcc tattagaaga tatccagatg ttttagtaca tagaattcta tatagttttt
      961 tacaaggtaa aactattatg gataaaaaaa tggaagagaa atgcaggcat tgtagcgaaa
     1021 gagaaaaggc tgccatggat tgcgaacgtg caggaaatag ttataagcag gtggagtttt
     1081 taaaagaaca tttgggcgaa gattttgaag gtgtaattag tggagtggca agttttggat
     1141 tttgggtaga aactttagcc cataaatgcg aaggaatggt aagtattgta agccttagcg
     1201 attatgacga ttttaaattt gtagaaactg atttttgttt agtgggcaaa agaagtggta
     1261 aaacttttag aataggtgat aaagtaacca ttaaagttgt tgcagctaat ttagaaaaaa
     1321 gaaaattaga ttatgaatgg gtaaccaatg ataatatgga agctacaaaa cctgtaaaaa
     1381 agaaaatcaa aaagcaaaag taaattaatg gctatttgct tgtaaccatt gttttaacct
     1441 aggaaatgca attctaaacc aagttgtaaa ttgctcaggt gttgtagcaa ttaaatcttc
     1501 aatggcatct aaagctacaa atttgaaatc tgctacttct tgttcgttaa tactaatttc
     1561 tccattgtat tctcctaaca aaacatggtc gtattcatgt tcggataaac cattttcaaa
     1621 agccacatta taaataaatt gaaagattgg ttttaatttg gttgtaaaac ccatttcttc
     1681 ttgtagtctc ctttctgctg catttgttac attttcatta ggtcttggat gactgcaaca
     1741 tgtattggtc cataagcctg cactatggta tttatttaaa gacctttgtt gtaataaaag
     1801 gtcaccattt ttatttacaa taaaaacact aaatgctcta tgcaataagc cagcttggtg
     1861 cgtttttatt ttttcttcaa taccaatttc attatcatat tcatctacta atattacctt
     1921 ttccatgcta taaaaataag gattgagtaa taaagtttat caactgtttt aatttttatc
     1981 ggtatgtaag tatccttgta aagcttttac atatatttca aatgcatgta ctccttgttt
     2041 agtaattgaa catgtagtta agggataatt atctttaaat tgtttggtta cttcaatata
     2101 accagcatct tttaattttt gtatttgaac agataaattg cctgcagtag attttgtttt
     2161 ttcttttata aaattaaaat cagcttcctt taccccaata agtaaactaa ttattgcaag
     2221 ccttagttga gagtgtaata taggatctag gtctttaaac attgctgttt ttttgttgta
     2281 aataatttct tcttaaaatt aaaccaggaa ttaaccaaca agaaatagct gctattgtac
     2341 ctgctaacat atcgtatttt gtgctggtat aacaagatat aataaacaaa acataggtta
     2401 aaattgcacc cactatcatg ggtttataat tttttacaat accagttgta aaagttggta
     2461 aagcatataa taaaataaaa agagaatagc tgcttggtgc aaatggggtt aatggttcat
     2521 cgggtttatt ggcatctaaa taatgtttag taaactgcca accttcttgc gtagcaagct
     2581 ttatacttga gttaggaata atatttaagt ataatactaa acccataata gaaattccaa
     2641 atgctaacca aacagcatta attgcagcat cttcaaactt tgttacctta ctctctttgg
     2701 ctgcttttat tgaaatataa atttgaaatg caatggcaat aattgttaaa agaaacgggc
     2761 taaaacttaa tttaaaattt aactcaatac ttaaataagt taataaagaa gcaattgcta
     2821 taacacagcc ccaaagtata gcattagttc cggtttcatg ataacttgct tttgcatttt
     2881 gtatcatgga tgttattaaa tctaaacttt ctttttctgt taatggttta ttactcttca
     2941 taactattgt ttttatattt agcttttaat aaatatcctg gaacaatcca tgctgccaaa
     3001 acagctaatg ccaatagtag taaaacataa atatctgcag taaactttgc tataaaagcc
     3061 aaaccccagc aaacaattcc acctattttt aatggtaaaa accttatggc aacaccactt
     3121 ataaaagtag gtgtgccata cataaataat attatgggat ataaagtaat ccaattattc
     3181 tgattaccaa ttattataga aataaagaac atggtaaatc caaaccccat ccaaacatag
     3241 cctacaattt catcggtata agatttaact tttttcttct tttctttttt taggatgtaa
     3301 tagatttggt aaataacagc tccccaggtt aaaacccaaa tatattctgg atacttaatt
     3361 atattagttt gcaaaagtat aaaatggcca attgagcata taaaaataac ccaaccccat
     3421 aaaaggtata aagtgctatc atcgctataa ttgcttttgg ctttgttaat catgttttca
     3481 attatagcca aactttctgt aggagttaat tctttttcca ttgtaacact tttaataaat
     3541 ttggtaaata ttttacttgc aattttttaa aatttgttca accatttctt ttccccaatt
     3601 tggatgaatt tcagatgcag gtttaaaact attaaacaac tccattgctt tttgtgcaat
     3661 aggtttggca gcattacaac caccaccata ctgttctggt gtgttaacta agttactcga
     3721 ttctaaaata tacggacgag gattattggc atctgctttt tttgcttctg ctaaatattt
     3781 gtttattaat gtgccaaatt ccatatacct gttttgtgga tctacaatca ttttagcagt
     3841 tgcaatcata cttttaatta ctaaaatttc acaattgttt ttagataatg cctctgcttt
     3901 atcaattaaa gattgagcct cgtctgcaac ttcatcttta tctccaccaa ttcccataat
     3961 actcatttta gcttttatgg tagctgcata ataataaggt agccattctg ttttctcagc
     4021 ttcggcaata cgaataaatt tttgagcaat agcaatgttt tcttctgctg ttttagctgc
     4081 ctcatactct ttaatgctac tgctcattgc tgcattaaat ttgtcggatt gggcaataat
     4141 acttgtacta ataaatagtg ttaagcataa gatgattttt ttcatgatgt gtgttttagt
     4201 tgtttgtaaa ttttataaat tattatttat agcatcttgt gttttatcaa ctccccaact
     4261 taaaaagcag ccaataaaat aaaactgctt ggctgtaggg gtaacagcaa ctttatttaa
     4321 attattata
//
LOCUS       DAOQHP010000174         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12052_length_4327_cov_3.430712, whole genome shotgun sequence.
ACCESSION   DAOQHP010000174 DAOQHP010000000
VERSION     DAOQHP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_4327_cov_3.430712"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLY81_06410"
     CDS             <1..774
                     /locus_tag="PLY81_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNF29895.1"
                     /translation="LIGQEPLAQLAEVFKHTYIAQINWTHHVILLDKIKTTDERLWYI
                     RKTIENKWSSTVLLHQIESGLYQRQYKTNKTTNFHLTLSKTQSDLALEIFKDPYKFDF
                     LSLTERITEKGLENNLVNQITKFLLELGNGFAFIGNQYKVKTPRKAYAIDMLFYHLKL
                     RAYIVIELKMEAFSPGDASQLNFYLNLVDDFVKDKNDKPSIGILMCKAKDNFEVEYAL
                     RGLNKPIGVAEYKMLPKKIREQLPSSTQFRNMLKKIKNL"
     gene            818..1783
                     /locus_tag="PLY81_06415"
     CDS             818..1783
                     /locus_tag="PLY81_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF29896.1"
                     /translation="MKQFALIGAAGFIAPRHMKAIKDTGNDIVCALDNFDSVGILDSY
                     FPNADFFTEFERFDRHIEKLRLNKTPIDYVSVCSPNYLHDAHIRFGLRNNTDVICEKP
                     IVLNPWNIDALIDMEKQTGKKIFNILQLRLHPAIIELKEKVANADANKVYDIDLTYIT
                     SRGNWYYTSWKGNIEKSGGIATNIGVHFYDMLQWIFGEVKQNIVHIHEHDRAAGYLEF
                     KQARVRWFLSIDYNTIPQALRNEGKRTYRSLTINDESFEFSDGFTELHTNSYKHILSG
                     AGFRIEEARTAINIVHHIRTQKPIGLQGNYHPFAKLPLSKHPFSK"
     gene            1815..2471
                     /locus_tag="PLY81_06420"
     CDS             1815..2471
                     /locus_tag="PLY81_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF29897.1"
                     /translation="MIKLNLGCGSVRPEGWINTDSSLNANIQRIPFIGKLVTKTFGKV
                     QYSSSNFIYMNLNKKWQYADNSVDIVYASHLFEHLTQKAADLFLKEAYRTLKVGGTLR
                     IVVPDLYQIATKYLAEYDTKNDNEDTTKFIMWAINMHREGQYGNPSILRKIIYEWQGY
                     PHQHKFMYDPKSLSLKVKNYGFNNIHLMSYGKSEYIKEILDVEGTSESYLSVYLECKK
                     "
     gene            2667..2897
                     /locus_tag="PLY81_06425"
     CDS             2667..2897
                     /locus_tag="PLY81_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29898.1"
                     /translation="MKTALQIDLTFDQILSVIKQLPKQQKIMLSKELEKEVIDSKLSR
                     LLKTFHTKELDLKTITKEVEIVRQDAYAKQKH"
     gene            2878..3297
                     /locus_tag="PLY81_06430"
     CDS             2878..3297
                     /locus_tag="PLY81_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNF29899.1"
                     /translation="MPNKSIKVIFDTNIWISFLIGKHLAKIKRYLSNGSIIIITSEQL
                     ISEIKVVTGRAKLKKYFPHDSVEEMIELLETIAIKVETKSTHFVSRDPKDNFLLDLID
                     FSKADFLVTGDKDLLELSPFKTAQILTPTEFETQLKN"
     gene            3327..3590
                     /locus_tag="PLY81_06435"
     CDS             3327..3590
                     /locus_tag="PLY81_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29900.1"
                     /translation="MKNLAIIITHPIQYYAPVFQLLAKCCTIKLFYTFGEKVLKDKYD
                     PGFGKIITWETSLLISTHESYLSVYLEYKNNENINLCSSVQSY"
     gene            3550..>4327
                     /locus_tag="PLY81_06440"
     CDS             3550..>4327
                     /locus_tag="PLY81_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF29901.1"
                     /translation="MKISICVVQYNRIELLLKSLSIIEKQTYHDIEICISDDCSTDNT
                     VDEILKLKQTYKYPIVFHRNEVNKGFDFNYRKSIEIASGDYAFVIGNDDSINGENSIA
                     ELVKFLEENNYPDLGFCNMLEERTGNTVIKRAMNTTILGSGAGVAIKYYSCFVFVGGL
                     IYKRNTFLKYNTDQYDGSVYTQMYLSVFMICKGERAFSIKEPMVLKDILLDGKFRHSY
                     RDRIAKKWNKFKVVYGGLPSVMHVVINGIRDAGLLEQKHIY"
BASE COUNT         1669 a          558 c          720 g         1380 t
ORIGIN      
        1 ctaattgggc aagaacctct tgcccaatta gcagaagttt ttaaacatac ttatattgct
       61 caaattaatt ggacacatca tgtaatatta ttggataaaa ttaaaactac tgatgagcga
      121 ttatggtaca ttcgtaaaac aatagaaaat aaatggagta gtacagtatt gctgcaccaa
      181 atagaatcag gcttatacca aaggcagtat aaaacaaaca aaacaaccaa ctttcatctt
      241 acgttatcta aaacgcaaag cgacttagct ttagagattt tcaaagatcc ttacaaattc
      301 gattttcttt ctttaactga aagaattaca gagaagggat tagaaaataa tttagttaat
      361 caaataacca aatttttgtt ggaattaggt aatggttttg catttatagg taatcagtat
      421 aaagtaaaaa cgccacgtaa agcctatgca atagatatgt tgttttatca cttaaaacta
      481 agagcatata ttgttataga attaaaaatg gaagcgtttt cgccaggcga tgcaagtcaa
      541 ctaaattttt atttaaacct tgttgatgat tttgtaaaag acaagaacga taagccttct
      601 attggaatat taatgtgtaa ggcaaaagat aattttgaag tggagtatgc attaagaggc
      661 ttaaacaaac caattggtgt agcagagtat aaaatgcttc ctaaaaaaat aagagagcaa
      721 ttaccaagct ctactcaatt tcgtaatatg cttaaaaaaa ttaaaaattt ataacaatat
      781 caactaaaaa agtgaggagg taaataaaat ataatacatg aaacaatttg ctcttattgg
      841 tgctgcaggt tttattgcac cacgacacat gaaagctatt aaagatacag gcaacgatat
      901 tgtgtgtgct ttagataatt ttgatagcgt aggtattttg gatagttatt ttcccaatgc
      961 tgattttttt acagagtttg aacgttttga caggcatata gaaaaattac gtttaaataa
     1021 aactccaatt gattatgttt ctgtttgctc acccaattat ttacatgatg cacatatacg
     1081 ttttggttta agaaataata cagatgttat ttgcgaaaaa cctattgtat taaacccttg
     1141 gaatattgat gctttaatag atatggaaaa gcaaacaggc aaaaaaatat ttaatatttt
     1201 acaactcaga ttacatccag ccattataga attaaaagaa aaagtagcta atgcagatgc
     1261 taataaagtg tacgatatag atttaactta cattacttcc agaggcaatt ggtattatac
     1321 cagttggaaa ggaaatatag aaaaatctgg tggtattgct actaatattg gagtgcattt
     1381 ttacgatatg cttcaatgga tttttggcga agtaaaacaa aatattgttc atatacatga
     1441 gcatgataga gcagcaggtt atttagagtt taaacaagca agagtacgtt ggtttttaag
     1501 tatagattat aataccatac cacaagcatt aagaaatgaa ggtaagagaa cctatcgttc
     1561 tttaacaata aacgatgagt cgtttgagtt tagtgatggc tttacagaat tgcacaccaa
     1621 cagttataaa cacattttat caggtgcagg ttttagaatt gaggaagccc gaacagctat
     1681 aaatattgtg caccatattc gtacacaaaa accaataggt ttacaaggta attatcatcc
     1741 ttttgcaaaa ttacctttaa gtaaacatcc ttttagtaaa taaaattggt taagaaatta
     1801 tttaaattca aaaaatgata aaattaaatt taggctgtgg ttcagttaga ccagagggtt
     1861 ggattaacac agattcttct cttaatgcaa atattcaaag aattccattt attggaaaat
     1921 tggttacaaa aacatttggt aaagtacaat acagcagttc taattttatt tatatgaact
     1981 taaataaaaa atggcaatat gctgataatt ccgttgatat agtatatgcc tcacatttat
     2041 ttgagcacct tacccaaaag gctgcagact tatttttaaa agaagcatac agaactttaa
     2101 aggttggtgg aactctgaga attgttgtac ccgatttata ccaaattgca actaaatatt
     2161 tagcagagta cgatacaaaa aatgataatg aagatactac caaatttata atgtgggcaa
     2221 ttaatatgca cagagagggg caatatggca acccaagtat tttaagaaaa attatttatg
     2281 aatggcaggg ataccctcac cagcataaat ttatgtacga ccccaaaagt ttaagtttaa
     2341 aagtaaaaaa ttatgggttt aataatattc acttaatgag ttatggtaaa agtgagtaca
     2401 ttaaagaaat actagatgta gaaggtacaa gtgagtcgta tttatctgtt tatttagaat
     2461 gtaaaaaatg attaatttct tgcacctcgc catcatcatt acacacccta ttcagtacta
     2521 tgcaccagtg ttttatactt ggtgctaaga ggtattgaaa gataaatatg accctggttt
     2581 tggtaaggtg attaaatggg atttaccttt attggaagac agcaaatatt gtagtaagtt
     2641 tgataaaata tttaattaaa taaatgatga aaacggcatt gcaaatagac ctaacttttg
     2701 atcaaatatt atctgtaata aaacaattac ctaaacagca aaaaataatg ttatcaaaag
     2761 agttagaaaa agaagttatt gatagcaaac tatctcgttt gttaaaaaca tttcatacaa
     2821 aagaacttga tttgaaaaca ataacaaagg aagttgaaat tgtaagacaa gacgcctatg
     2881 ccaaacaaaa gcattaaagt aatttttgat actaatattt ggattagctt tctaattgga
     2941 aaacatttag ctaaaattaa acgatacctc tctaacggaa gtatcattat tataacttca
     3001 gagcaattaa tttcagaaat taaagtagta actgggagag caaaattaaa gaaatacttc
     3061 cctcatgata gtgttgaaga aatgattgaa ctcttggaaa ctattgctat aaaagtggaa
     3121 acaaaatcaa cacattttgt aagtagagac ccaaaagata attttctttt agacttgatt
     3181 gatttttcaa aagcagactt tttagtaaca ggcgataaag atttattaga actcagccct
     3241 tttaaaactg ctcaaatcct gacacctact gaatttgaaa ctcaacttaa aaattagtgt
     3301 atagaaaatt tgtttgaaat atacacatga aaaaccttgc catcatcata acacatccta
     3361 ttcagtatta tgcaccagta tttcagttat tagctaagtg ttgtactata aaattgtttt
     3421 atacttttgg cgaaaaggtt ttgaaagata aatatgaccc tggttttgga aaaataatta
     3481 catgggaaac ttctttattg ataagcactc atgagtcgta tttatctgtt tatttagaat
     3541 ataaaaataa tgaaaatatc aatttgtgta gttcagtaca atcgtattga attattgtta
     3601 aaaagtttgt caattataga aaagcaaact tatcatgata tagaaatttg tatcagcgat
     3661 gattgctcta ccgataatac tgttgatgaa atcctaaaac taaaacaaac ttataaatat
     3721 cctattgtat ttcatagaaa tgaagtaaac aaaggatttg attttaatta cagaaaaagt
     3781 attgaaatag catcgggtga ttatgctttt gttattggta atgatgattc cataaatgga
     3841 gaaaacagca ttgcagaatt ggtaaagttt ttagaagaga ataattatcc agatttaggc
     3901 ttttgtaata tgctggaaga aagaacaggc aatacagtta ttaaaagagc aatgaataca
     3961 acaattttag gttcaggtgc tggtgttgca ataaaatatt acagttgctt tgtttttgta
     4021 ggagggctaa tttataaaag aaacactttc ttaaaataca atacagacca atatgatgga
     4081 agtgtttata cacaaatgta tttaagcgtt tttatgatat gcaagggtga aagagcattt
     4141 agtattaaag aacccatggt attaaaagat attttgttag atggcaaatt cagacattct
     4201 tacagagaca gaatagcaaa aaaatggaat aagtttaagg tggtatatgg tggtctccct
     4261 tctgtaatgc atgtagttat taatgggatt agagatgcag gattattaga gcaaaaacat
     4321 atatata
//
LOCUS       DAOQHP010000175         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12112_length_4314_cov_3.290444, whole genome shotgun sequence.
ACCESSION   DAOQHP010000175 DAOQHP010000000
VERSION     DAOQHP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_4314_cov_3.290444"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="glgP"
                     /locus_tag="PLY81_06445"
     CDS             <1..959
                     /gene="glgP"
                     /locus_tag="PLY81_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNF29902.1"
                     /translation="FCGLTVDEVKKLTGQEEDQFNHSLVALRFARKANAVSQLHGVVS
                     NKMWSKYTNICPITAITNAQNFSYWADEPLYRHLDANNDWGIDDRKSYLKKRAFENVA
                     DQTGKLFNPEVLTIVWARRFAGYKRADLLTHDMERFEKLVNNKNFPVQIIWAGKPCPA
                     DYPAISQFNELVHLSKKYKNVSVMIGYELLLSRRLKQGADVWLNNPRVPREASGTSGM
                     TAAMNGAVNFSTDDGWIPEFAKPGTNSFVIPKADYENMTQHDQDIYDLYRMYDILESE
                     IIPMYYNDKDAWRQMVKNGMNDVRVQFNSNRMAHEYYEIMYK"
     gene            complement(1166..1453)
                     /locus_tag="PLY81_06450"
     CDS             complement(1166..1453)
                     /locus_tag="PLY81_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF29903.1"
                     /translation="MEEKTKINIEEGVVHNLPDDIKEVLITNIDLLEKWNKLTPLARN
                     EWICWVTIVKQEHTRKEHILRLQEDILSGKKRPCCWPGCPHRRESAKKWFK"
     gene            1865..2428
                     /locus_tag="PLY81_06455"
     CDS             1865..2428
                     /locus_tag="PLY81_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNF29904.1"
                     /translation="MNKIIVAITGASGSIYAKLLLDSLMQMKNQFAEIGVVMSDNAKT
                     VWQTELKNEDYKNYDVTFYSKNNFNAPFASGSAKFNIMIVAPCSMGTLGRIASGISDD
                     LITRAADVILKERRKLILMVRETPFNLIHLKNMETITLAGGIICPAIPSFYSLPTSIE
                     CLAKTVTDRVLELAGLEINTFRWGKQL"
     gene            complement(2434..3189)
                     /locus_tag="PLY81_06460"
     CDS             complement(2434..3189)
                     /locus_tag="PLY81_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29905.1"
                     /translation="MQNIVISVKDLNKKYGNFHAVKGISFEVFEGEIFGLLGPNGAGK
                     SSTLEIIETLRPKTSGTVKVVGFDLDTQPNFIKQQIGVQLQTSGFYPGLNLVELIDLF
                     AGLYNEKVNAKELLEQVNLQDKAQAKYKELSGGQKQRFSIATTLINKPKVIFLDEPTT
                     GLDPQARRNLWQLIKKIQAEGTTIIITTHYMDEAEILCNRVAIIDDGKIIAIDTPDKL
                     IDNLVATGFERPKEVKKANLEDVFIHLTGHSIR"
     gene            complement(3222..3725)
                     /locus_tag="PLY81_06465"
     CDS             complement(3222..3725)
                     /locus_tag="PLY81_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29906.1"
                     /translation="MKKLFLLLTVFIFFQANAQKIQEKLFYHSAKGLSFIPPLSKPGL
                     IYEGKLYIGKQRLTGLFYKLNDETLNSYFNKYKANKTAADILTITGGVALPLANIFIA
                     ANEGKVNWWLIAASALLNGTGGYLNMQAQKNLLLSSIYFDKKMGYTTNNFKPKQQVVQ
                     IAIPIGK"
     gene            complement(3729..4223)
                     /locus_tag="PLY81_06470"
     CDS             complement(3729..4223)
                     /locus_tag="PLY81_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNF29907.1"
                     /translation="MKQFHKLLIFTLTFIPLFIACGSTKESTNSELDEVSIAKAERII
                     LELVNKHRKKNDLPKLKAIDELTEIAEKHSKNMGDGKVKVGHGGFDNRYNKIKKEIKN
                     VGSAAENVAFGNTSLKKIVDGWIASPHHKGNIEGPFNYTGVGIYRNKQGVLFYTQIFI
                     NKVK"
BASE COUNT         1448 a          636 c          656 g         1574 t
ORIGIN      
        1 atttctgtgg cttaacagta gatgaagtga aaaagctaac aggccaagaa gaagatcagt
       61 ttaatcattc actggttgct ttaaggtttg caagaaaagc caatgctgta tcacaattac
      121 atggtgttgt aagtaataaa atgtggagta aatacacaaa tatttgtcct attactgcaa
      181 taaccaacgc acaaaatttt agttattggg cagatgaacc tttgtataga catttagatg
      241 ctaataacga ttggggcatt gatgatagaa aaagctattt aaagaaaaga gcttttgaaa
      301 acgtagcaga tcaaaccgga aaattattta atccggaggt tttaaccatt gtttgggcaa
      361 gacgttttgc aggttataaa agagccgatt tactgacgca tgatatggaa cgttttgaga
      421 aattagtaaa taataaaaac tttccggtac aaataatttg ggcaggaaaa ccttgtccgg
      481 cagattatcc agcaatttct cagtttaatg aactggttca tttaagtaaa aaatataaga
      541 acgtttctgt aatgatcggt tacgaattat tattaagccg aagattgaaa caaggggcag
      601 atgtatggtt aaacaaccct cgtgtaccaa gagaagcaag tggcacaagt ggtatgacag
      661 ctgccatgaa tggtgctgta aactttagta cagatgatgg atggattcct gaatttgcta
      721 aacctggtac taatagcttt gttataccta aagcagatta tgaaaacatg acccaacacg
      781 atcaagatat ttatgattta tacagaatgt acgatatttt agaaagtgaa ataattccta
      841 tgtattacaa tgataaagat gcttggagac aaatggtaaa aaatggaatg aatgatgtaa
      901 gagttcaatt taatagcaac agaatggctc acgaatatta cgaaataatg tataaataat
      961 agtgtataga aaaaccaaaa agcaaaactc cgaaatattt cattcggagt ttttttgtac
     1021 acttcaacct ttgtaaaaag taatttagaa tgtaataaat ttataaacaa ataatagatg
     1081 cttttattta ttaaatagtt gcaagtaatt taaatgtgta taaattttat aaaattcatt
     1141 tagctttttt gtataataag tgagattatt taaaccattt ctttgcactc tctctacgat
     1201 gtggacaacc tggccaacaa caaggtcgtt tcttaccact caaaatatct tcttgtaatc
     1261 gtaatatatg ttcttttcta gtatgttctt gttttactat tgtaacccaa caaatccact
     1321 cattacgagc aagtggagtt agtttgttcc atttttctaa aaggtcaata ttagtaatta
     1381 atacttcttt aatatcgtct ggtagattat gtacaacccc ttcttctatg tttatttttg
     1441 ttttttcttc cattttttat ttcatttttc taattaatta aaaattaatt attatttatt
     1501 aatgtgctgg aattacgttg gcttgctatt aagctaattg gcttctaata taaatgtaat
     1561 ttttctttac taattatgtt aggttaaaaa attttaaaga agattgaagc tgcaaaaatt
     1621 gtgatgactt ttgtagagaa gagtagagta atcgtttaca ttagttcata tcttacatta
     1681 tttgatggta gattatttac taatattact ataaataaaa agagggctta ggatagaaat
     1741 cctgccctgt ttaaatccaa tattctatga aaaaccatat tatagacgat gcatacagtt
     1801 taaaggttgc atcaagtatt aatgttttaa aaattacttt taatgtttaa tgtatttttg
     1861 gaacatgaat aaaattattg tagcaataac aggggcaagt ggtagtattt acgctaaatt
     1921 actattagat agtttaatgc aaatgaaaaa tcaatttgcc gaaattggtg ttgttatgag
     1981 cgataatgcc aaaacagttt ggcaaactga acttaaaaat gaagattata aaaactatga
     2041 tgtaactttt tactctaaaa ataatttcaa tgcacctttt gcttctggct ctgcaaagtt
     2101 taatattatg atagttgctc catgtagcat gggcacttta ggcagaatag catcgggtat
     2161 aagtgatgat ttaataacca gggctgctga tgttattttg aaagaaagac gaaaactaat
     2221 tcttatggtt agagaaacgc catttaattt aattcactta aaaaatatgg aaacaatcac
     2281 cttggcaggt ggtattatct gccctgcaat tccatcattt tattctttac ctaccagcat
     2341 cgaatgtctt gctaaaactg taacggatag ggttcttgag ttggcaggtt tagagataaa
     2401 tacatttagg tggggtaaac aattgtaaaa aatttatctt atagagtggc ctgttaaatg
     2461 aataaatacg tcttctaaat tagctttttt tacttcttta ggtctttcaa aacctgtggc
     2521 aactaagttg tctattaatt tatctggtgt atcaatagca attatttttc catcatctat
     2581 aattgctact ctattacaca gtatttcagc ttcatccata taatgagtag taataataat
     2641 ggtagtacct tctgcctgaa tttttttaat taattgccac aaatttcttc tggcttgagg
     2701 gtctaaacct gttgttggtt catctaaaaa aataactttt ggtttattaa taagtgttgt
     2761 agcaatagaa aaacgctgct tttgcccacc acttaattct ttatattttg cttgggcctt
     2821 atcttgtaaa ttcacctgct ctaataattc tttagcattc actttttcat tgtataatcc
     2881 agcaaataaa tcaatcagtt caactaaatt taaaccaggg taaaaaccac ttgtttgaag
     2941 ttgaacacct atttgttgtt taatgaaatt aggctgtgtg tctaaatcaa aaccaacaac
     3001 ctttacagtg ccacttgttt ttggcctaag ggtttcaata atttctaaag tacttgattt
     3061 acctgcaccg ttaggcccca acaaaccaaa aatctcacct tcaaatacct caaaactaat
     3121 tccttttact gcatgaaaat tgccatattt tttatttaaa tcttttacac taataactat
     3181 attttgcata taatagaatt gctttctttt tagaattaaa attatttacc aatgggtatt
     3241 gctatttgta ctacttgttg tttgggttta aaattattag tagtataacc cattttttta
     3301 tcgaaatata tggatgaaag taatagattt ttttgagctt gcatattcaa atatccacca
     3361 gttccattta acaaagcact tgctgcaata agccaccaat ttactttgcc ctcattggct
     3421 gcaataaaaa tattggctaa aggcaaagca acgccaccag taattgttaa aatatctgca
     3481 gcagttttat tagccttata tttattgaag tagctattta atgtttcgtc atttaattta
     3541 taaaacagcc ctgtaagcct ttgcttacct atataaagtt taccttcata aattaaacct
     3601 ggctttgata atggtggaat aaagcttaag cctttggcac tatggtaaaa tagtttttct
     3661 tgaatttttt gggcattggc ttgaaaaaaa ataaatactg taagcaataa aaataatttc
     3721 ttcattagtt attttacttt attaatgaat atttgggtat aaaataacac accttgttta
     3781 tttctataaa ttccaacacc tgtataatta aaggggcctt ctatattacc tttatgatgt
     3841 gggcttgcaa tccaaccatc tacaattttt tttaaactgg tatttccaaa agcaacattt
     3901 tctgcagcac ttccaacatt tttaatttct ttttttattt tgttatatct attgtcaaaa
     3961 ccaccatgtc ctacttttac ttttccatct cccatatttt tactatgctt ttcagctatt
     4021 tcggttagtt catcaattgc ttttaatttt ggtaaatcat tttttttccg atgtttattt
     4081 accaattcta aaattattct ttctgcttta gcaatactaa cttcatccaa ttcagagttg
     4141 gtagattctt tagtgctacc acaagcaata aataaaggaa taaaagttag tgtaaaaatt
     4201 aaaagtttat gaaattgttt cataaatttt ttttgtgaag gtagtattaa aattgttttt
     4261 aaacattaat catacttcct ttttttagca gcattaaaat attctagctg ttcc
//
LOCUS       DAOQHP010000176         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12155_length_4306_cov_3.629969, whole genome shotgun sequence.
ACCESSION   DAOQHP010000176 DAOQHP010000000
VERSION     DAOQHP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12155_length_4306_cov_3.629969"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLY81_06475"
     CDS             complement(<1..1038)
                     /locus_tag="PLY81_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNF29908.1"
                     /translation="MEGTTDESFNTTQTTSGYCFSQLRSYRFAVACTGEYAVAVGGST
                     PALLHSAIVTTVNRVNGVYEQEVAVRLILISNNNLIEFLNPSTDPFNGNDNAGVLINE
                     SQSVITSTIGTSNFDIGHTFSTGGGGLAGLGVVCISGSKGRGITGSSNPTGDAYDIDF
                     VAHEVGHQFGGSHTFNSTTGSCGGGNRSASSAVEPGSGVTIMGYAGICGSNDLQPHSI
                     PNFHSKSQEQIGTYAFTGIGSTCGTLIATGNNAPVVNAGSDYTIPANTPFMLTGSATD
                     ANGDVMTYSWEQYDLGTAGNWNSGNNPLFRSFEPTLSPTRYFPKKQDILDGTTTIGEM
                     LPPSARTMSFRL"
     gene            complement(1084..1575)
                     /locus_tag="PLY81_06480"
     CDS             complement(1084..1575)
                     /locus_tag="PLY81_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29909.1"
                     /translation="MKFYYKTLLLILVVCPILTMAQNNFFTNKNESQFAGSQDLVRPI
                     KPERFRAVSLDVTGLKQFLSTVPMEFSADASTRKSILTLPMPNGGTANFSIVESPMQE
                     PGLAAKFPDIKTYSGQGIDDKTATIKLDWTGFGFHAQILSPISGSIYIDPYSRGNVEN
                     YMS"
     gene            complement(1697..2452)
                     /locus_tag="PLY81_06485"
     CDS             complement(1697..2452)
                     /locus_tag="PLY81_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNF29910.1"
                     /translation="MLKDFIIALQAYRKAHLFIQKYKLWKWIILPGLFYCILFLVGMY
                     FFGNTANSFIEWLKGFLINSLNKVNSSFLNFVVTLSGIMIWVILMLLYFSFFKYFFLI
                     VGSPIFAYLSEKTASIIEGKDYPFNFSQILKDIVRGIRISLRNALWQTVYLFSILIIG
                     MIPLVGWFVPVLALLIECYYYGFSMLDYSMERNKKSVFESVNYIGVHKGLAIGNGFVF
                     YTMHLLPVIGWVLAPAYAVIAATLSIYDLKEKS"
     gene            2512..3126
                     /locus_tag="PLY81_06490"
     CDS             2512..3126
                     /locus_tag="PLY81_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNF29911.1"
                     /translation="MLTQAFYTNKNTIDVAKNLLGKILVTKMEGLVTKARIVETEAYC
                     GITDKACHAYNNKRTKRTEIMFANGGVCYIYLCYGMHYLFNVVTNTINNPHAVLVRAV
                     EPLEGLEIMQKRTGKKENDFTITKGPGNVTKALGITNNQNGYSLLQNTIYIEEDDFIL
                     SSKQIINTPRIGVHYAKEDALLLYRFFIKNNLFVSGKKIDNFAN"
     gene            3136..3813
                     /gene="upp"
                     /locus_tag="PLY81_06495"
     CDS             3136..3813
                     /gene="upp"
                     /locus_tag="PLY81_06495"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNF29912.1"
                     /translation="MKRFKKLNMVINLSSKNSLVNNWISELRDINVQNDRLRFRRNLE
                     RFGEVAAYEISKNFNFKTVEIQTPLGTHNSNVIAEQPVLATILRAGLPLHNGMLNYFD
                     KADNAFISAYRKHHRDGSFEISLEYVSCPSLDNRTIIISDPMLATGASLVKTIAILQD
                     RWQINKIYVVCAIASTVGIEYVERECGNNIEIWCGDIDEELTAKGYIVPGLGDAGDLA
                     FGTKAQS"
     gene            3896..>4306
                     /locus_tag="PLY81_06500"
     CDS             3896..>4306
                     /locus_tag="PLY81_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF29913.1"
                     /translation="MKKLIIVLLIFILGFSANAQDPHFSQFFSSPLTLNPAFTGKFNG
                     NYRIAGNYRNQWPTINNAFITSTASVDFQLMQKKLVYTDNWGVGIMGYTDNSANGAVK
                     FNYASLSTAYHKGLDEDGYNQIGVGFQITYANMLI"
BASE COUNT         1542 a          732 c          650 g         1382 t
ORIGIN      
        1 aagtctaaaa ctcattgttc ttgcacttgg tggtaacatt tctccaatag tagttgtacc
       61 atctaaaatg tcttgttttt taggaaaata tctagtcgga gataaagttg gttcaaaact
      121 tctaaataaa ggattattgc ctgagttcca attacctgca gtgcctaagt cgtattgttc
      181 ccaactataa gtcattacat ctccattagc atctgttgca ctacctgtta acataaaagg
      241 tgtatttgca ggaatggtat agtcagaacc tgcattaaca actggtgcat tattacctgt
      301 agctattaaa gtgccacaag tacttccaat acctgtaaat gcataagtac caatttgctc
      361 ttggcttttt gaatgaaagt ttggaatact atgaggttgt aaatcattgc taccacaaat
      421 tcctgcataa cccataatag ttacaccact accaggctct actgcagatg aagcacttct
      481 attaccacca ccacagctgc cagttgttga gttaaaagta tgactacctc caaactgatg
      541 acctacttcg tgagctacaa agtcaatatc ataagcatca cctgtagggt tggaactacc
      601 tgtaatacct cttcccttac taccactaat acacactaca cctaaaccag ctaaaccacc
      661 accaccagta ctaaaagtgt ggccaatatc aaaattagat gttccaattg tgcttgtaat
      721 tactgattgg ctttcgttaa taagtacacc tgcattatca ttaccattaa atggatctgt
      781 acttggattt aaaaactcaa ttaaattatt gttgctaatt aatattagcc taacagctac
      841 ttcttgttca taaactccat ttaccctatt aacagtagtt acaatggcag agtgcaaaag
      901 ggctggcgtg ctacctccta cagcaactgc atattctcct gtacaagcta ccgcaaacct
      961 ataagatcta agttgagaaa aacaatatcc acttgttgtt tgtgtagtgt tgaaagattc
     1021 atctgtagtt ccttcaatac ctacttcttg aaattgacta gagggttgta agtctttctt
     1081 aaattaactc atataatttt ctacattccc tcttgagtaa ggatctatat atatgctacc
     1141 acttattgga gataaaattt gagcatgaaa accaaagcct gtccagtcta gtttaatagt
     1201 tgcagttttg tcatcaatac cttgcccact ataagtttta atatcaggaa attttgcagc
     1261 taaaccaggc tcttgcattg gagactcaac aatactaaaa ttagctgtac caccattagg
     1321 cattggtaaa gttaatatag attttctggt tgaagcatct gcagaaaact ccataggtac
     1381 agtgcttaaa aattgcttta ggcctgtaac atctaatgat acagctctaa atctttctgg
     1441 ttttatgggt ctaaccaaat cttgagaacc agcaaactga ctttcatttt tgttggtgaa
     1501 aaagttgttt tgtgccattg ttaaaatagg gcaaacaact agaataagca gtaaggtttt
     1561 gtagtaaaat ttcatatata aaatttaagt tctgcaaatt aattaaaagg ccttattcta
     1621 acaacaatat tattagaaat accttcttat taaattagga aaatatgctt ctattgatta
     1681 gaaacataaa gttacattag cttttttctt ttaaatcata aatactaagt gtagctgcaa
     1741 ttactgcata agcaggggcc aaaacccatc ctattactgg taataaatgc atagtataaa
     1801 aaacaaaacc attacctata gccaaacctt tatgcactcc aatataattt acactttcaa
     1861 aaaccgattt cttattacgt tccatgctat aatctaacat tgaaaatcca taataataac
     1921 actcaattaa aagagctagt accggaacaa accaacccac taacggtatc attcctataa
     1981 ttaaaatact aaacaaataa actgtttgcc acaaagcatt tcttaaagat attcttatac
     2041 ctcttacaat atcttttaat atttggctaa aattaaacgg gtaatctttt ccttctatga
     2101 tagaagctgt tttttcactc aaatatgcaa aaattggcga gccaactatt aaaaagaaat
     2161 atttaaagaa tgaaaaatat agtaacatta aaataaccca aatcataatg ccacttagtg
     2221 ttactacaaa gtttaaaaag ctgctattaa ctttatttaa agaatttatt aagaagcctt
     2281 tcagccattc aataaaacta ttagcagtat taccaaaaaa atacatgcct actaaaaaca
     2341 aaatgcaata aaatagccct ggtaaaatta tccatttcca tagtttatat ttttgtataa
     2401 acaaatgtgc ctttctatag gcttgtaatg ctataataaa gtcttttaac attaatctta
     2461 atatttccgt tgctaagata ccaaagcaat taacaataaa tagaatattt tatgctaacc
     2521 caagcatttt ataccaataa aaacacaata gatgttgcaa aaaatttgtt aggtaaaata
     2581 ctagttacca aaatggaagg cttagttacc aaagccagaa ttgtagaaac agaagcatac
     2641 tgtggcatta cagacaaagc ctgtcatgca tacaataata aaagaacaaa acgtacggag
     2701 attatgtttg ctaacggtgg tgtatgttat atatatttat gctatggaat gcattattta
     2761 tttaatgtag taactaatac aattaataac ccacatgcag ttttggttag agctgttgaa
     2821 ccattagaag gcttagaaat aatgcaaaaa agaacaggaa aaaaggaaaa cgattttaca
     2881 ataactaaag gtccaggcaa tgtaaccaag gctttaggca taacaaacaa tcaaaatgga
     2941 tatagccttt tgcaaaatac aatttatata gaggaagatg attttatact gtcttcaaaa
     3001 caaattatta ataccccaag aataggagta cattatgcta aagaagatgc actattatta
     3061 taccgatttt tcattaaaaa caatttattt gtgagcggaa agaaaattga caattttgcc
     3121 aattaacaat tagctttgaa gcgttttaaa aaattaaata tggtaataaa cttaagcagt
     3181 aaaaacagtt tagtaaacaa ctggattagc gaattaagag atattaatgt acaaaacgac
     3241 aggttgcgtt ttcgtagaaa ccttgaaaga tttggcgaag tggcagccta cgaaataagt
     3301 aaaaatttta attttaaaac agtagagatt caaacgccat taggcacaca taatagtaat
     3361 gtaattgcag aacaacccgt tttagcaact attttaagag caggtttacc tttacacaat
     3421 ggcatgctta attattttga taaagcagat aatgctttta tttctgctta tagaaaacac
     3481 caccgtgatg gcagttttga aataagctta gaatatgtaa gctgcccttc tttagataac
     3541 agaacaatta ttattagcga cccaatgctt gcaactggag catctttggt taaaacaatt
     3601 gctattttac aagatagatg gcaaataaat aaaatatatg ttgtatgtgc aattgccagt
     3661 actgttggta ttgaatatgt tgaacgtgaa tgtggtaata atattgaaat ttggtgtggc
     3721 gatattgatg aggaattaac tgcaaaaggc tacattgtac caggcttagg cgatgctggc
     3781 gatttagcat ttggcaccaa agctcaatct taataaaaac ctattactaa aatattcatc
     3841 aagtaatttt attcatttac aacatttcac ttactttcgg catcttttaa ttttcatgaa
     3901 gaagttaatt attgttttat tgatttttat tttgggtttc agtgcaaatg ctcaggatcc
     3961 tcacttttct caattttttt catcgccact tacacttaac cctgcattta caggtaagtt
     4021 taatggcaat taccgcattg caggtaatta cagaaaccaa tggccaacta taaataatgc
     4081 atttattaca agtacagcat ctgttgactt tcagttaatg caaaaaaaat tagtttatac
     4141 agataattgg ggagttggta taatgggcta tacagataat agtgcaaatg gtgcagttaa
     4201 atttaattat gcttctttat ctactgccta tcataaagga ttagatgaag atggctacaa
     4261 tcaaataggt gttggctttc aaattaccta tgccaatatg cttata
//
LOCUS       DAOQHP010000177         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12246_length_4284_cov_3.886262, whole genome shotgun sequence.
ACCESSION   DAOQHP010000177 DAOQHP010000000
VERSION     DAOQHP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12246_length_4284_cov_3.886262"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1119
                     /locus_tag="PLY81_06505"
     CDS             244..1119
                     /locus_tag="PLY81_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF29914.1"
                     /translation="MLETKEINALFTLIDDPDEEVYSTVSNRIIGFGKDIIPNLENLW
                     ENTLSEAVQSRIEMLIHSLHFTDLKQSFIDWRDSAYADLLTGALLVAKYQYPDLHTTP
                     VLQDIEKIRRNVWLELNNYLTPLEQANVLSSIIYNYYNLKGGEVNYNNVDEFFIHKAL
                     ESKKGNAISNGILYQIVCDKLDINAKIINIPKQMILAFYHKDLDLDKSKTSPQDKILF
                     YVDGVNGQAYGHQDIDNYFKRISVPPVPAYFKPISNKRIIQVLLDETGKCFNTTSNLY
                     KQTELTQLANILDEQ"
     gene            1174..1794
                     /locus_tag="PLY81_06510"
     CDS             1174..1794
                     /locus_tag="PLY81_06510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF29915.1"
                     /translation="MYLAKTYWWLKALYPTFIWNKSREEKVIYLSFDDGPNPTVTPFV
                     LDLLKQYKAKATFFCIGKNVVHFPALYHQIINEGHSVGNHTQHHLNGWQTKTEKYIHN
                     IQTAALQIQSNLFRPPYGRIKRAQANKLTKNSSNPFQIIMWDVLSGDFDTNISGEQCF
                     INVTNNTENGSIIVFHDSDKAWERLSYTLPKILELYSKQGFEFKAL"
     gene            complement(1816..2148)
                     /locus_tag="PLY81_06515"
     CDS             complement(1816..2148)
                     /locus_tag="PLY81_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNF29916.1"
                     /translation="MNWLLLILAGIAEVSFTFCLGKAKQATGMEQIIWYVGFFIALII
                     SMLLLMKAIETIAIGTAYAVWTGIGAVGTVLIGIFVFKEPTNFWRLFFLMLLISSIIG
                     LKVVATKN"
     gene            complement(2185..2616)
                     /locus_tag="PLY81_06520"
     CDS             complement(2185..2616)
                     /locus_tag="PLY81_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF29917.1"
                     /translation="MLTSLFIVDDDPITVKICEMVIKNTEFASAVTHFENGQKCINFF
                     SNYFEKNLDKSKNVVMPELILLDLNMPVLDGWGFLDHYERKYANRLTETKIAILTSSI
                     NPQDFMKAQQYSNIIDFIYKPLLTNIVEELKEHEALKMYFS"
     gene            complement(2644..3132)
                     /locus_tag="PLY81_06525"
     CDS             complement(2644..3132)
                     /locus_tag="PLY81_06525"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNF29918.1"
                     /translation="MSVAKFSIEIDEFKSIEELDAADVKLLQLAQKIAENAYAPYSNF
                     YVGAVAMLNNNQIITGTNQENASYPVGTCAERVLLAIAATSFPNIPLKTMAISYLNKN
                     GLNNHPISPCGMCRQYLLEYEQRVKQPIRLILGGQVGKVYIIKSVADLLPLNFSADDF
                     KK"
     gene            complement(3202..>4284)
                     /gene="lepB"
                     /locus_tag="PLY81_06530"
     CDS             complement(3202..>4284)
                     /gene="lepB"
                     /locus_tag="PLY81_06530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNF29919.1"
                     /translation="MEKTLLVNDFLFVNKMAYGPKVPQTPLSFPFVHNLMPGSQKPSY
                     LKWIQLGYKRVPGYDKLHRNDVVVFNFPEGDTIINLPEYGSLVTYYSLVRQYGRDAVW
                     NRFENDIIVHPADKTDNYIKRCVAVAGDTLEVKDGIVYINKVKQPIPKFAQRTFIVNA
                     TKAGLNFDDLKSNYNIDIRTDQYYLAYQDSINASFARTGMAEIIMTEEDAAKIKQLKG
                     VIKVEPAIEQYDAFAAQAYYFPFDSLHHHFSRDNYGPIYIPKAGAKIDLNTNTLSLYY
                     RAITSYEGHTLQVNGNEILVDGKPTTTYTFKYNYYWMMGDNRHNSQDSRFWGFVPETQ
                     IVGRASLIWFSWDKGPRWKRLFNIIK"
BASE COUNT         1521 a          652 c          612 g         1499 t
ORIGIN      
        1 tctcttcatt attaaataat ttgttgcaaa attaataaaa ccattgtaat tgcctaaaat
       61 ttgtaggttg taacatataa tctcaataat tattgtatgc ttttttgcca acatttccac
      121 agtggtggaa tacttctgtt ttattaatta gttagaatta tcgtagtttg aattgtgttt
      181 atgattattc atcacttaaa cgctattgtt taaaatgatt ttcatgaact aaaaatagca
      241 cctatgcttg aaaccaaaga aattaatgca ttgtttacat tgattgatga tcctgatgaa
      301 gaagtatata gtaccgtttc taacaggatt ataggctttg gaaaagacat tataccaaac
      361 ttagaaaatc tttgggaaaa tacccttagc gaagctgtgc aaagcagaat tgaaatgctt
      421 attcacagtc ttcattttac cgatttaaaa caaagtttta ttgattggcg agatagtgcc
      481 tatgccgatt tattaactgg tgcattactg gttgctaagt atcaataccc agatttacac
      541 acaacccctg ttttacagga tattgaaaaa attagaagaa acgtttggct tgagctgaat
      601 aattatttaa cacctttaga acaagccaat gtattaagta gtattattta caattattac
      661 aacttaaaag gaggggaggt aaactataat aatgtagatg aattttttat acataaagct
      721 ttagaaagta aaaagggcaa tgcaatttct aatggaattt tatatcaaat tgtttgcgat
      781 aaattagata tcaatgcaaa aattataaat attcctaaac aaatgatatt agctttttat
      841 cataaagatc ttgatttaga taaaagcaaa actagcccac aagataaaat attattttat
      901 gtagatggag tgaatgggca agcatacggc catcaggata tagataatta ttttaaaaga
      961 atttctgtac cgcctgtgcc agcttatttt aagcctattt caaacaaaag aattatacaa
     1021 gtattattag atgaaacagg caaatgtttt aatacaactt ctaatttgta taaacaaacc
     1081 gaattaacac aacttgccaa tattttagat gaacaataaa ttgaatttat ttttactttt
     1141 aaaatataaa caataattat ttaatacaga tacatgtatt tagctaaaac atattggtgg
     1201 ttaaaagcat tgtatccaac ttttatttgg aataaaagca gagaagaaaa agtaatatac
     1261 ctaagttttg atgatggccc aaacccaaca gtaacaccat ttgtattaga tttattaaag
     1321 caatacaaag ccaaagcaac atttttttgt ataggtaaaa atgtagttca ttttccagca
     1381 ctttatcatc aaataataaa cgaaggccat tcagtaggta atcacacaca acaccatttg
     1441 aatggctggc aaactaaaac agaaaaatat atccacaata ttcaaactgc tgccttacaa
     1501 atacaatcaa atctttttag accaccctac ggtagaatta aacgagcaca agcaaataaa
     1561 ttaaccaaaa attctagtaa tccttttcaa ataataatgt gggatgtttt aagtggagat
     1621 tttgatacta atatttcagg cgaacagtgc tttattaatg ttaccaataa taccgaaaat
     1681 ggaagtataa ttgtttttca tgatagtgat aaagcatggg aaagattgag ttatacttta
     1741 cctaaaatac tggagttgta tagcaaacaa gggtttgaat ttaaagcctt gtaattgata
     1801 tagtatcttt ttgctttaat tttttgtagc taccactttt agtccaataa ttgaagatat
     1861 aagtagcatt aaaaaaaata atctccaaaa atttgttggc tctttaaaaa caaaaatacc
     1921 aattagtact gtaccaactg ccccaatacc tgtccatact gcataggctg taccaattgc
     1981 tatggtttca attgctttca ttaataagag catgcttata ataagggcaa taaaaaagcc
     2041 tacataccaa ataatttgtt ccatgcctgt tgcttgtttt gctttaccta aacaaaaagt
     2101 aaaactaact tctgctattc ctgctaaaat caaaagcaac caattcatag taaaaaatga
     2161 gagtaaaatt gaatatagat tgtattaact aaagtacatt tttaatgctt catgttcttt
     2221 tagttcttca acaatattag ttaataaggg tttgtaaata aaatcaataa tattgctata
     2281 ttgttgtgct ttcataaaat cttgtgggtt aatactagag gttaaaatag caatctttgt
     2341 ttctgtaagc ctattagcgt attttctttc ataatggtct aaaaaacccc acccatctaa
     2401 tacaggcatg tttaagtcta ataaaattaa ttcaggcatt acaacatttt tacttttatc
     2461 taggttcttt tcaaaataat tagaaaagaa gttgatacac ttttggccat tctcaaaatg
     2521 agttacagca ctagcaaact ctgtattttt gataaccatt tcacaaattt ttactgtaat
     2581 agggtcatca tctacaataa ataatgaagt aagcatgttg ttttgattta aaaaattaaa
     2641 aaatcatttt ttaaaatcat ctgcactaaa attaagtggc aataaatctg caacactttt
     2701 aataatataa actttaccta cttggccacc taaaattaat cttataggtt gttttactct
     2761 ttgttcatac tctaataaat actgcctgca cataccacat ggtgaaatag ggtggttgtt
     2821 caatccattt ttattcaaat aactaatagc catagttttt aatggaatat tgggaaagct
     2881 tgtagctgca atagctaata aaactctttc ggcacaagta cctacagggt aagaagcatt
     2941 ttcttgattg gtacctgtta ttatttggtt attgtttaac attgctactg cacccacata
     3001 aaagtttgaa taaggtgcat aagcattttc ggctattttt tgagccaatt gtaaaagttt
     3061 tacatcggct gcatctagtt cttctatact tttaaattca tctatttcaa tactgaattt
     3121 agctacggac atacattatt ctttttaatt aataagctat taaagaaata tataaaagct
     3181 tatctgatag gaaagataag actattttat gatattaaat aatcttttcc atctagggcc
     3241 tttatcccaa ctaaaccata ttaaggatgc tctacctaca atttgagttt cgggtacaaa
     3301 accccaaaat ctgctatctt ggctattatg cctattgtca cccatcatcc aataataatt
     3361 gtatttaaaa gtataggtgg tagtaggttt cccatctact aaaatttcat tgccatttac
     3421 ttgaagagta tggccttcgt agcttgtaat ggctctgtaa tatagagata gtgtatttgt
     3481 gtttaaatca atttttgcac cagctttagg tatataaatt gggccataat tatctcttga
     3541 aaaatgatga tgtaaagaat cgaaaggaaa ataataagct tgtgctgcaa atgcatcgta
     3601 ttgttctatt gcaggttcta ctttaataac tccttttaat tgttttattt ttgcagcatc
     3661 ttcttcagtc ataattattt cagccattcc tgttctggca aaagaggcat tgatagaatc
     3721 ttgataagcc aaataatatt gatctgttct tatatcaatg ttatagtttg attttaaatc
     3781 atcaaaattt aagcctgctt ttgtagcatt tactataaag gttctttgtg caaattttgg
     3841 aatgggttgt ttcactttat taatatacac tatgccatct ttcacttcta aagtatctcc
     3901 tgcaacagct acacaacgtt taatataatt atctgtttta tcggcagggt gaacaataat
     3961 atcgttttca aatctattcc aaactgcatc tcttccatat tgtcttacta aagaataata
     4021 agtaactaac gaaccatact ctggcaaatt aataattgta tctccttcgg gaaaattaaa
     4081 aacaacaaca tcgtttctat gtaatttatc atagcctgga actcttttgt aacctagctg
     4141 tatccatttt aagtatgatg gcttttgaga gccaggcatt aaattatgaa caaaaggaaa
     4201 acttaatgga gtttgaggaa cttttggccc ataagccatt ttgtttacaa ataaaaagtc
     4261 gttaaccaat aaagtttttt ccat
//
LOCUS       DAOQHP010000178         4273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12298_length_4273_cov_3.858464, whole genome shotgun sequence.
ACCESSION   DAOQHP010000178 DAOQHP010000000
VERSION     DAOQHP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12298_length_4273_cov_3.858464"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLY81_06535"
     CDS             complement(<1..880)
                     /locus_tag="PLY81_06535"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29920.1"
                     /translation="MKHLRKFLLKNRIAPKWAVFGLDMLIACFAIVYANYLRFNFDFQ
                     FITLNDLIDDLVIVIVTNSILFYIFKTFHGIIRFSGFQEAFRSIAAIFYSFFIMIVMN
                     VILSSLQLPQFTPISVLFIYFFIASFLLFGYRLLVKHLYSISINNEEDTHVVIYGAGQ
                     NGSLLKKTIEQTSNNRYKIVAFIDDEEDLIGKTIDTVKIYPVSHLQMTFKNWQIKTLF
                     FAKQDFDLDEKNKIVDYCLESNVKVMNIPPMKEWIQGHLNVNQLKEVKIEELLGRKQI
                     SLRNEHVINHLRDKKIL"
     gene            complement(1096..1695)
                     /locus_tag="PLY81_06540"
     CDS             complement(1096..1695)
                     /locus_tag="PLY81_06540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNF29921.1"
                     /translation="MDSIRLLDILFSIIGIIILLPLFILIALCIKLTSKGSIIFKQVR
                     VGKNNTDFKLLKFRTMVAFAEAKGQLTVGGKDNRITAIGYYLRKFKLDELPQLINVLM
                     GQMSLVGPRPEVRKYVALYSEQQIEILKVLPGITDFASIEFRNENELLANAIHPEEFY
                     IKEIMPKKIELNKKFIKDRTIYNYFLILFKTVFTAIKGK"
     gene            complement(1688..2908)
                     /locus_tag="PLY81_06545"
     CDS             complement(1688..2908)
                     /locus_tag="PLY81_06545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF29922.1"
                     /translation="MQIPFSPPFINENIELEVLDALRSGWITSGPKVKALEQEVCNYT
                     NAEACLCVNSATSAMMLVLHWYGVKKGDEVIVPAYTYCATALAVMHLGATPIMVDVED
                     DFNISVSKIKLAISSRTKAIMPVDFAGWPCNYSEINKLINEPEIKQKFKAETEQQKKL
                     QRILVLSDAAHSFGAVYNNKKLGTAADITIFSFHAVKNLTTAEGGAICLNLPLPFNNN
                     DVYATLRLWSLNGQTKDAFSKSKAGSWRYDIVYPGFKMNLPDVLAAIGLAQIKEYNEV
                     LLKERKRVFDFYSKTFSNYSWAMLPPYDVPNASSSYHLYPLRIKDINEEQRDRIIEKI
                     AETGVAVNVHFQPLPLLTVFKQMGYDINNFPSAYNNYKAEISLPIYPQLNNNKCTYIV
                     DNVVNAIKEVLING"
     gene            complement(3088..3432)
                     /locus_tag="PLY81_06550"
     CDS             complement(3088..3432)
                     /locus_tag="PLY81_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29923.1"
                     /translation="MFRIKNIQKISVAVVLLLAVQVLYATNIFSGGIERKNEVSKYSL
                     KNLSRYNNKTLTLSTAKYSYKLKSTLLNNNSTLNNSRSSILLSNGNTTFIYPYKSKVK
                     VPKFKTPSPTKF"
     gene            3563..>4273
                     /locus_tag="PLY81_06555"
     CDS             3563..>4273
                     /locus_tag="PLY81_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF29924.1"
                     /translation="MKDQNHSHEDREAMNALLMHYHNLKNGRNHPFIEEEDFERIIDY
                     FDDNNNINEALEAAEISISQFPYSSTLLLRKADLLIATKKYIEALEILNQAALYDSTD
                     IGLYILRTDALLALDKQEEAVEVLAEALQLFEGNEKLDLLFELADVYDDFEEFDKVYD
                     CLKLILEEDPNNEEALYKICFWTDFTGRNEESINLYKKIIDDFPYNEIAWFNLAAAYQ
                     GLKLYEKAIDAYQFAIVID"
BASE COUNT         1537 a          620 c          588 g         1528 t
ORIGIN      
        1 ttaatatttt tttatctctt aaatgattaa taacatgttc atttcttaag ctaatttgct
       61 tgcggcctaa aagttcttca atttttactt ctttaagttg attgacgttt aaatgccctt
      121 gaatccactc cttcataggt ggaatgttca ttactttcac attgctctct aaacaataat
      181 caactatttt atttttttcg tctaagtcaa aatcttgctt ggcaaaaaat aaggttttaa
      241 tttgccaatt cttaaacgtc atttgtaaat gactaacagg ataaattttt acagtatcaa
      301 ttgttttacc tatcaaatct tcttcgtcat ctataaatgc tacaatttta tacctgttat
      361 ttgaagtttg ttctattgtt ttctttaata atgatccatt ttgcccagca ccgtaaataa
      421 ctacatgtgt gtcctcttcg ttattaatac ttatactata taaatgtttt accaataacc
      481 tatatccaaa taataaaaag gaagcaataa aaaagtaaat aaataaaact gaaatgggtg
      541 taaactgtgg caattgtaat gaacttaaaa ttacattcat tacaatcatg ataaagaaag
      601 aataaaaaat agctgcaata cttctaaatg cctcttgaaa accagagaat cttataatac
      661 catgaaaggt tttaaagata taaaacaata tgctattagt tacaataaca ataactaaat
      721 catctattaa atcatttaaa gtaataaact gaaaatcgaa attaaaacgt aaatagttgg
      781 catatacaat ggcaaaacag gcaataagca tatctaagcc aaaaactgcc cacttgggtg
      841 caattctgtt ttttaataaa aatttcctca aatgtttcac ttattaagta cgttttgctt
      901 taataacgat ttttttttat aaatattgga tgtactaaag tgtttttctt agaaggcaag
      961 taaaataaga tggatttaag ccaatacaaa tatagtttaa aattttataa cactcttgtt
     1021 ttttaaaacg taaactaatc ttacttaaca tgcaatctac catagtacaa actctatgta
     1081 ttatgttgtt atttattatt tgccttttat agctgtaaat acagttttaa aaagtattaa
     1141 aaaataatta tagattgttc tatctttaat aaatttttta ttaagttcta tttttttagg
     1201 cattatttcc ttaatataaa actcttccgg atggatagca tttgccaata attcgttttc
     1261 atttctaaat tcaatggagg cgaaatctgt aatacctggt aatactttta atatttcaat
     1321 ttgctgctca gaataaaggg ccacatattt tctaacttct ggtcttggtc ccaccaaact
     1381 catttgcccc attaatacat ttattaattg tggtaattcg tctagtttaa attttcgtaa
     1441 atagtaacca attgcggtaa ttctattatc ttttccacca actgtaagtt gtccttttgc
     1501 ttctgcaaaa gctaccatcg ttctaaattt aagtaattta aaatcggtat tatttttccc
     1561 aactctaact tgtttaaaaa taatacttcc tttagaagta agtttaatac aaagagcaat
     1621 tagaatgaat agtggtagca atattattat tcctatgata gaaaaaagta tatccaatag
     1681 tctgatacta tccatttatt aaaacttctt ttattgcatt caccacatta tcaacgatgt
     1741 aggtgcattt attattgttt aactgaggat aaattggtaa agagatttct gctttgtagt
     1801 tattatatgc cgaaggaaaa ttattaatat cgtagcccat ttgcttaaaa acagttaata
     1861 aaggtaatgg ttgaaaatgg acatttacag caacgcctgt ttcggcaatt ttttcaataa
     1921 ttctatctct ttgctcttca tttatatctt ttattcttaa ggggtataaa tgataactag
     1981 aagaagcatt aggtacatca taaggtggta acattgccca tgaatagtta gaaaatgttt
     2041 tggaataaaa atcaaaaact ctttttcttt ccttcaataa tacttcgtta tattctttaa
     2101 tttgagctaa gccaatagca gccaaaacat ctggcaaatt catcttaaag ccagggtaaa
     2161 caatatcata tctccagcta ccagctttag acttagagaa tgcatctttt gtttgtccgt
     2221 ttaaactcca aagccttaag gttgcataaa catcattatt attaaatggt aaaggaaggt
     2281 ttaaacaaat agctccaccc tcggcagtag ttaagttttt tactgcatga aaagaaaaaa
     2341 tagtaatatc agcagcagtt cctaattttt tattattata cacagcacca aaagagtgtg
     2401 cagcatcgct taacactaaa attctttgta actttttttg ttgctcagtt tcggctttaa
     2461 atttttgctt tatttctggc tcattaatta atttatttat ttcagaataa ttgcaaggcc
     2521 atccggcaaa atcaacaggc attattgctt ttgttctgct actaattgcc aatttaattt
     2581 ttgaaactga gatattaaaa tcatcttcaa catctaccat tattggtgtt gcacccaagt
     2641 gcatcacagc taaagcagta gcacaataag tatatgcagg cacaattact tcatcgcctt
     2701 ttttaacgcc ataccaatgc aaaacaagca tcattgccga tgtggcagag tttacacaca
     2761 aacatgcttc agcattagta taattacaaa cctcttgctc taaggcttta acttttggtc
     2821 ctgaagttat ccaaccactt cttaaagcat ctaaaacttc taactcaata ttttcattga
     2881 taaaaggcgg agaaaatggt atttgcataa atatatataa tcaatttttt aaacgtaccc
     2941 gaaattaaag gaaatgaaaa gtaaggaata aaatttaggg ataaatattt tgatgtaacg
     3001 ggtttataaa cgcaaaaaaa taaaatatta ttttatatgc agaaataata atacacgaat
     3061 taagaaaaaa agaaagaagt taattaatta aaactttgta ggagaaggtg ttttaaattt
     3121 aggcactttt actttagatt tataaggata tataaaagta gtattgccat tagaaagcaa
     3181 aatagatgaa cgagaattat ttaaagtaga attgttattt agtaaagtag attttaattt
     3241 ataactatac tttgcagtac ttaaagttaa agttttattg ttatagcggc taaggttttt
     3301 tagagaatat ttacttactt cattcttacg ctcaatgccg ccagaaaaaa tgttagttgc
     3361 atacaatact tgtactgcaa gtagtaaaac cacagcaact gatatctttt gtatgttctt
     3421 aattctaaac atttttacaa aaatagttat tttaaaaata tttaacaaat tttatgttat
     3481 taacaagacg ttgaaaagta taaaaagtta catattagat taaatttttt ttcctatatt
     3541 taatttagtt attcatttta taatgaaaga tcaaaatcat tcacacgaag acagggaggc
     3601 aatgaatgca ttattaatgc attatcataa ccttaagaat ggaagaaatc atccttttat
     3661 agaggaagaa gattttgaac gtattataga ttactttgat gacaataata atattaacga
     3721 agcattagaa gcagcagaaa taagtattag ccaattccct tactcctcta ctcttttatt
     3781 aagaaaggcc gatttattaa tagcaaccaa aaaatatatt gaagccttag aaattttaaa
     3841 tcaggctgcc ctttacgata gcacagatat tggattatac attttaagaa cagatgctct
     3901 tttagcttta gataaacaag aagaagcagt tgaagtttta gcagaagctt tacaactttt
     3961 tgagggcaat gaaaagttag atttgttgtt tgaacttgca gatgtatatg atgattttga
     4021 agaatttgat aaagtatatg attgcttaaa acttatttta gaggaagacc ctaataacga
     4081 agaggcttta tataaaattt gtttctggac agattttaca ggaagaaatg aagaaagcat
     4141 taatttatac aaaaaaatta ttgacgattt tccctacaac gaaattgctt ggtttaacct
     4201 tgcagcagct tatcaaggtt taaagttgta tgaaaaagcc atagatgctt atcagtttgc
     4261 aatagtaatt gat
//
LOCUS       DAOQHP010000179         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12338_length_4265_cov_3.465796, whole genome shotgun sequence.
ACCESSION   DAOQHP010000179 DAOQHP010000000
VERSION     DAOQHP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12338_length_4265_cov_3.465796"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLY81_06560"
     CDS             complement(<1..392)
                     /locus_tag="PLY81_06560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29925.1"
                     /translation="MKPISFIIISYNRVDDTLALLQNISKLSKAADLLEEVIVVNNAS
                     TVDYNKVKEFADNSKTISFKYYDAPSNLGVTKGRNYALQFAQGEILIFLDDDALLQNE
                     DALENVLKAFEGKGLDNREIAIVSFKVL"
     gene            complement(505..1611)
                     /locus_tag="PLY81_06565"
     CDS             complement(505..1611)
                     /locus_tag="PLY81_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNF29926.1"
                     /translation="MKKLFLFYSTLLISSALIFTACGKKSSGGSGSTSSITSLTIAAN
                     KSTLTADGFDKVTFSVTDQAGNDVTAQTIIYLNNYSSAGYSRIFSVGEHGTYKVTAKK
                     GTVTSNEITLTVNAAAAPQYSTKVIAEDFTGAWCGWCPRLTYKFENFMANNHKIITIG
                     VHNGDALALSSESSLRSKLNPKVTSFPTVFLNRTREFNDNGNINSFADSTDIKEFLQK
                     RSPLGLAINSTISGSNLNITTKVGFDANISDKLRLVVMVLEDGKVLSQSNYYHGNASY
                     PGNPYYSIGNPATSFVHNGVLRAVPTGIFGEDIVTADQTKNNIKTYNHTVSIAGMNPA
                     NLRVVAFVNFFDATYAGKIINAQVAPANSNKNFD"
     gene            1711..2166
                     /locus_tag="PLY81_06570"
     CDS             1711..2166
                     /locus_tag="PLY81_06570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF29927.1"
                     /translation="MFSTLIFSQENLKEVTLKDAKGNPIQFSEVIKADKPVIVSFWAT
                     WCAPCLQELEAINDVYEEWQKTTGVTLYAVCIDDARSQSKAASIVKSKGWNYEIVYDP
                     NHDLKRILNVVNVPNLLVYYKGNVIYKHSGYSPGSEDILYEKIKSAIKK"
     gene            2186..3868
                     /locus_tag="PLY81_06575"
     CDS             2186..3868
                     /locus_tag="PLY81_06575"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNF29928.1"
                     /translation="MKKISILLISGLFVLNVSNAQDKQNKKEKVKVSGAVESNAQYYN
                     EKDDKLGFVAPNNRFRSNNYLKLNVSYKGFSTGGQFEAYAPDVLLGYPENLKGSGLTN
                     YYVAYNSKKISATIGHYYEQFGSGLVLRSWEDRQLGINNALFGAKVQYTPTDAVSFKA
                     IYGKKREAYSLSEGVVWGTDAELNFTKLFKQNEQQKGSFTTGFSYVGKQEKYTGNIAN
                     FPETVHLFSLRTSYQKNNLILNAEYALHTKDGCVNDLGNVVNTNKFFNGNVLQLGGTV
                     SGKNSAFNFTLRQVKNFGMYTDRKAKLNQLLLNYVPALTAQHHYSLSNIYVYNPQVKF
                     SFLPGNLLNNTGEIGGQIEWLKTFPKKSKLGGKYGMFLKINLSHYSGLAFDGTDVNTA
                     KVYNFKAAKTNFRDFNIEVKKTWNAKVKTNFSFINMLYNQKAIEGYGSDNIKTNILVA
                     EGIFKLKKGHSIKADIQHLWAENDYRGNWVAATFEYGIAPMLNFFVADMYNYGNSVKQ
                     VHYYNAGASFTKGTFRFMASYGRQREGLLCVGGVCRLVPASSGFTFTTSYSF"
     gene            complement(3941..>4265)
                     /locus_tag="PLY81_06580"
     CDS             complement(3941..>4265)
                     /locus_tag="PLY81_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNF29929.1"
                     /translation="SIKAMKWTKLSTEQAGAFYAIHKERPFYRELVEFMSSGPIVAAI
                     LEKENAVADFRTLIGATNPANAEEGTIRKKYAASVGENAVHGSDSDENAIIESNFFFS
                     ELERY"
BASE COUNT         1496 a          620 c          675 g         1474 t
ORIGIN      
        1 tataaaacct taaagcttac aatggctatt tctctgttat ctaatccttt tccttcaaat
       61 gctttcaaaa cattttctaa ggcgtcttca ttttgcaaca aagcgtcatc gtctaaaaaa
      121 atcaatattt ctccttgtgc aaattgtaat gcataattcc ttcctttggt tacacctaaa
      181 ttacttggtg catcataata tttaaaagaa attgtttttg aattatctgc aaattctttc
      241 actttattat aatccaccgt tgatgcatta tttactacaa taacttcttc aagtaaatct
      301 gcagctttgc ttagtttact tatattttgc aataatgcaa gtgtgtcatc tactctgtta
      361 taagaaataa ttataaaact aattggcttc atgaaaaaca aatataacag ataatatttt
      421 ctttactttt tttgtggcta ccatacaata aaaaaagctg ctttaaaaag ttaaagcagc
      481 ttatagaaaa acagttataa aaaactaatc gaaatttttg ttggaatttg caggtgcaac
      541 ttgtgcatta ataattttac cagcataagt agcatcgaaa aaattaacaa atgctacaac
      601 ccttaaatta gcagggttca taccagcaat acttactgta tgattatatg ttttaatatt
      661 atttttggtt tggtctgcag taacaatatc ttctccaaaa atacctgttg gtacagctct
      721 aagaacgcca ttatgtacaa aacttgtagc tgggttacca atactataat atggattacc
      781 agggtaagaa gcattaccgt gatagtaatt agattgagat aaaactttac catcttctaa
      841 aaccattaca accaatctta atttatcact aatgtttgca tcaaaaccta ctttagttgt
      901 aatatttaaa ttagagccac taattgtaga gttgattgct aaaccaagtg ggcttctttt
      961 ttgtaaaaat tctttaatat cagtactatc agcaaagcta ttaatattac cattgtcatt
     1021 aaactctctt gttctgttta aaaatactgt agggaagctt gtaacttttg gatttaattt
     1081 gcttcttaag ctagattcag atgataaagc aagtgcatct ccattatgaa caccaatagt
     1141 tattatttta tgattattag ccataaaatt ctcaaactta taggttaatc ttggacacca
     1201 accacaccaa gcaccagtaa aatcttctgc aataactttt gtagaatatt gtggggcagc
     1261 agctgcattt acagttaaag taatttcgtt acttgtaaca gtgccttttt ttgctgttac
     1321 tttgtaagta ccatgctcac ccactgagaa aattcttgaa taaccagcgc tactataatt
     1381 gtttaaataa ataattgttt gtgctgttac atcatttcct gcttggtcgg ttacagaaaa
     1441 agtaactttg tcaaatccgt ctgctgttag agtagatttg ttggcagcaa ttgttaagga
     1501 tgttattgaa cttgtagaac cactacctcc gctacttttt ttgccacaag cagtaaaaat
     1561 taaagcactg ctaataagta atgttgaata aaaaaggaaa agttttttca ttgtttttat
     1621 tgtttttagt ataatattgt ccgcaaattt ataatctcaa aaggtttaac aatgattttt
     1681 aagaaattta tttttctaac atcatttttg atgttttcaa cattgatatt cagtcaggaa
     1741 aacttgaagg aggttacact caaagatgcc aaaggaaatc ctattcagtt ttcagaagtt
     1801 attaaagcag ataaacctgt aattgtatct ttttgggcaa catggtgtgc accctgttta
     1861 caagagttag aagcgataaa tgatgtatat gaagaatggc aaaaaactac aggggttaca
     1921 ttgtatgcag tttgtataga tgatgcaagg tctcaatcta aagcagcctc cattgtaaaa
     1981 agtaaaggat ggaattatga aatagtgtat gaccccaatc acgacttgaa aagaatttta
     2041 aatgttgtga atgtacccaa tttattggta tattataaag gaaatgttat atataaacat
     2101 agtggttact caccaggtag cgaagatatt ttatacgaaa aaattaaatc ggcaataaaa
     2161 aaataatact ttaattttca aataaatgaa aaaaattagc atactgctta taagtggttt
     2221 atttgttttg aatgtaagta atgctcaaga taaacaaaat aaaaaagaaa aagtaaaagt
     2281 atccggtgct gtagaatcta atgctcaata ttataatgaa aaagatgaca agttggggtt
     2341 tgtagcacct aataacagat ttcgttctaa taattatctt aaattaaatg tatcctataa
     2401 aggattttct acaggaggac agtttgaagc ttatgcacca gatgtgttat taggttatcc
     2461 tgaaaattta aagggtagtg gtttaacaaa ttattatgta gcttataatt ctaaaaaaat
     2521 tagtgcaact attggccatt attacgagca atttggaagt ggtttagtac tacgttcttg
     2581 ggaggatagg caattaggaa ttaacaatgc tttatttgga gctaaagtgc aatatacacc
     2641 aacagatgct gttagtttta aagctattta tggtaaaaaa agagaagctt attctcttag
     2701 cgaaggtgtt gtttggggaa cagatgctga gttgaatttt actaaattat ttaaacaaaa
     2761 tgaacaacaa aaaggtagtt ttactactgg ctttagctat gtaggtaaac aagaaaaata
     2821 tactggtaat attgcaaatt ttcctgaaac cgttcacttg ttttctttga gaacaagtta
     2881 ccaaaaaaac aatcttattt taaatgcaga atatgcctta catactaaag atggatgtgt
     2941 aaacgattta ggaaatgttg taaatactaa taaatttttt aatggtaatg tattgcagtt
     3001 aggtggtaca gtaagtggca aaaactctgc ttttaatttt actttaagac aagtgaaaaa
     3061 ttttggaatg tacaccgata gaaaagctaa attaaatcag cttttattga attatgttcc
     3121 tgcattaact gcacaacacc attattcttt atctaacatt tatgtttata atccgcaagt
     3181 taaattttct tttttaccag gtaatctttt aaataatact ggagaaattg gaggacagat
     3241 tgaatggtta aaaacctttc ctaaaaaatc taaactaggc ggtaaatatg gtatgtttct
     3301 aaaaataaac ttatcgcact attctggttt agcttttgat ggtactgatg taaatacagc
     3361 aaaagtgtac aactttaaag cagcaaaaac caattttaga gattttaata tagaagttaa
     3421 aaaaacatgg aatgctaaag taaaaaccaa tttttcattt ataaatatgt tgtataacca
     3481 aaaagctatt gaaggctatg gtagtgataa tataaaaacc aatatattag ttgcagaagg
     3541 tatttttaaa ttaaagaaag gccattctat aaaagcagat atacaacatt tatgggcaga
     3601 aaatgattat agaggaaatt gggttgctgc cacatttgaa tatggaattg caccaatgct
     3661 taactttttt gttgctgata tgtataatta tggaaatagt gttaagcaag ttcattatta
     3721 taatgctggg gcatctttta ctaaaggcac ttttagattt atggcttctt atggcagaca
     3781 aagagaagga ttactttgtg ttggtggtgt atgtcgttta gtacctgcat cctctggatt
     3841 tacatttaca acaagctata gcttttaaaa taaaagcata actacataaa aaaacgaggc
     3901 tcctaattaa agcctcgttt tttatttgag caaactatta ttaatatctt tctaattcag
     3961 agaaaaagaa attgctttca ataattgcat tttcatcgct atcgctacca tgtacagcat
     4021 tttcgccaac agaggctgca tacttttttc tgatagttcc ttcttcggca tttgcaggat
     4081 tagtagcacc aattaaagtt ctaaaatcgg caactgcatt ttctttttct aaaattgcag
     4141 caactattgg tccactactc ataaactcta ctaattctcg ataaaaagga cgttctttat
     4201 gaatagcata aaatgcacca gcttgctcgg ttgatagctt agtccatttc attgctttaa
     4261 tgcta
//
LOCUS       DAOQHP010000180         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12343_length_4264_cov_3.332858, whole genome shotgun sequence.
ACCESSION   DAOQHP010000180 DAOQHP010000000
VERSION     DAOQHP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12343_length_4264_cov_3.332858"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLY81_06585"
     CDS             <1..803
                     /locus_tag="PLY81_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNF29930.1"
                     /translation="LMDAGVPFKKHVSGVAMGLITRATDGKYAILTDILGDEDHLGDM
                     DFKVTGTKDGICGVQMDIKIDGLSMEVMSEALEQARKGRLHILDAMENCIAAARPEVK
                     PHTPRMEKLIIDKEFIGAVIGPGGKVIQEIQKVTGTTINIEEVGNTGEVSIFSQEKES
                     LLKAVEWVKGIVAVPQVGEVYDATVKSIKEFGAFVEFLPKKEGLLHISEISWKRLESM
                     DGIFKEGDKVKVKLLEIDQRTGKFKLSRKALMPRPERQDKPQQPTVEG"
     gene            863..1675
                     /gene="prmA"
                     /locus_tag="PLY81_06590"
     CDS             863..1675
                     /gene="prmA"
                     /locus_tag="PLY81_06590"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNF29931.1"
                     /translation="MNYIQLKIEVSNEAMQELVIALLADNGYNGFETTLTNVLAYIDE
                     IEYSESLVKKILQPMQIKFIATVIEKQNWNEIWETNFPSVLVDNFVGIRANFHQALHN
                     VQHEIIITPKMSFGTGHHETTFSVIQLMQEIDFNNKTVFDFGTGTGILAILAEKLGAK
                     KVVAVDNDEWCIENSIENVNANNCKNIIVQNAENAITNQKYDIVIANINKNIILQNIQ
                     ALHNAVKNNGFILLSGLLKDDEDEIKMVTSQIGWQPIKTIAKNNWIALCYTS"
     gene            1757..2419
                     /gene="plsY"
                     /locus_tag="PLY81_06595"
     CDS             1757..2419
                     /gene="plsY"
                     /locus_tag="PLY81_06595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNF29932.1"
                     /translation="MNEFLFIILAYLIGSIPTSIWVSKRFFNIDIREYGSGNAGATNT
                     YRVLGPKWGTFVMIIDMLKGVVATSLYIFLPSYLGISNEWDRTNLMIGLGLAAVVGHI
                     FPIWANFKGGKGVATLFGMAVAIQPLVAVCCIAVFLFVLYLTRFVSLSSILAGVAFMV
                     FILFIFNEETTLYRVFAVMVALMVILTHQKNIGRIIKGTEGKVPILKFRDNRKKRNRK
                     PF"
     gene            complement(2635..3018)
                     /locus_tag="PLY81_06600"
     CDS             complement(2635..3018)
                     /locus_tag="PLY81_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNF29933.1"
                     /translation="MNIYVGNLNWNMTSEDLQELFAPYGEVASAKIVTDKFKNNRSKG
                     FGFVEMNDDDAARAAISALNDTDVQDRKIVVNESTPRPEGERRSSGGGGGFRKSGGFG
                     GGNRGGGGGGYNRDRGGNGGGYNRY"
     gene            3306..>4264
                     /locus_tag="PLY81_06605"
     CDS             3306..>4264
                     /locus_tag="PLY81_06605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF29934.1"
                     /translation="MLPSVAIVILNYNGINYLQQFLPSVLSSTYSNKKIIVADNASTD
                     DSVNWVKENYPQITVLINTQNNGYAGGYNWCLKSVEADYFVLLNSDVEVTANWIEPII
                     ELMQADTKIAACQPKILSWHHKEKFEYAGACGGFIDLYGYPFCRGRIFDFCEDDKGQY
                     NSVEKIFWASGAALFIKSKVFVDLNGFDENFFAHMEEIDLCWRLQLSGYTIAVQPASI
                     VYHVGGGTLPKSYQKTFLNFRNNWIMLCKNLTIKEKFLIFPVRFFLDCTTACKALLSK
                     DTEMARAIFNAHIAFIKWKFSSKYKSVQPNKPLYKLNGVLLNS"
BASE COUNT         1484 a          571 c          753 g         1456 t
ORIGIN      
        1 cattaatgga tgctggtgtt ccatttaaaa aacatgtaag tggtgttgca atgggtttaa
       61 taacaagagc caccgatggt aaatatgcta ttttaacaga tattctaggc gatgaagatc
      121 atttaggtga tatggacttt aaagtaacag gcactaaaga tggtatttgt ggcgtacaaa
      181 tggatattaa aattgatggc ttaagtatgg aagtaatgag tgaagcatta gaacaagcca
      241 gaaaaggacg tttacatatt ttagatgcaa tggaaaactg tattgctgct gcacgtcctg
      301 aggttaagcc acatactcca agaatggaaa aactaattat tgataaagaa tttattggag
      361 ctgtaattgg ccctggtgga aaagtaattc aagaaattca aaaagtaaca ggtacaacta
      421 ttaatataga agaagtaggt aatactggtg aggtaagcat cttctctcaa gaaaaagaaa
      481 gtttacttaa agcagtagaa tgggttaaag gtattgtagc agtacctcaa gtaggagagg
      541 tttatgatgc cactgtaaaa agtattaaag agtttggtgc gtttgtagaa tttttaccta
      601 agaaagaggg cttattacat attagcgaaa tcagttggaa acgattagaa agtatggatg
      661 gaatatttaa agaaggagat aaagtaaaag tgaaattatt agaaattgac cagcgtactg
      721 gtaaatttaa actaagcaga aaagcattaa tgccaagacc agaaaggcaa gataaaccac
      781 aacaacctac tgttgaaggc taaagttgtt aatctaaatt aaattccccc gagtgtaata
      841 attcggggga ttatttttta aaatgaatta tatacaatta aaaatagagg tttccaatga
      901 agccatgcaa gagttagtaa ttgcattgct tgcagataat gggtacaatg gttttgagac
      961 taccttaacc aatgtgttag catatattga tgaaatagaa tacagcgaaa gtttggttaa
     1021 aaaaattctc caaccaatgc aaataaaatt cattgcaaca gttattgaaa aacaaaattg
     1081 gaacgaaata tgggaaacaa attttccgtc tgtattggta gataattttg taggtattag
     1141 agcaaatttt caccaagctt tacataatgt acaacatgaa ataattatca ctcctaaaat
     1201 gagttttgga acaggacatc atgaaactac attttctgta attcaattaa tgcaggagat
     1261 tgattttaat aataaaacag ttttcgattt tggcacaggc actggtatat tagcaattct
     1321 tgcagaaaaa ttaggtgcaa aaaaagttgt tgcagtagat aatgatgagt ggtgtattga
     1381 aaacagtatt gagaatgtaa acgcaaacaa ctgtaaaaat atcattgttc aaaatgcaga
     1441 aaatgcaatt actaatcaga aatatgatat tgtaatagca aacataaata aaaatattat
     1501 tcttcaaaat attcaggcat tacataatgc tgtaaaaaat aatggcttca ttttattaag
     1561 tggattattg aaggatgatg aagatgaaat aaaaatggtt acatctcaaa ttggctggca
     1621 acctattaaa actatagcca aaaacaattg gatagctttg tgttatacaa gttaataact
     1681 tgttgtttta ataaattgtg catttttatt tacaaacaga ttgtatattt gaattttcca
     1741 accttcaacc aacgcaatga acgagttttt gtttattatt ttggcatatt taattggctc
     1801 tatacctact tctatttggg taagcaaaag gttctttaat attgatatta gagaatatgg
     1861 aagtggtaat gctggagcaa ctaataccta tagagttcta ggacctaaat ggggtacttt
     1921 tgtaatgatt attgatatgc tgaaaggggt ggtagctact tccttatata ttttcttgcc
     1981 atcttattta ggaataagca atgagtggga tagaaccaat ttaatgattg gtttaggctt
     2041 ggctgctgtg gttgggcata tatttcctat atgggcaaat tttaaagggg gtaaaggtgt
     2101 tgcaacttta tttggaatgg cagttgccat acaacctttg gttgctgttt gttgtattgc
     2161 tgtattttta tttgttttgt atcttactcg gtttgtttca ttaagttcta ttttggctgg
     2221 tgtagctttt atggttttta ttttatttat ttttaatgaa gaaactacat tatatagggt
     2281 ttttgcagta atggttgcat taatggttat tcttacccat caaaaaaata taggaagaat
     2341 tattaaagga acggaaggga aagtgcctat tttaaaattc agagacaata gaaaaaaaag
     2401 aaataggaaa ccattttaaa tttatactcc tgaaatgctt tgctgtagtt tttgttcaat
     2461 ttctttattt gcatcaatac cattgtaatt tctattcaat taaaaaatag ttttgtgagg
     2521 tgcattaaac ataaaactta atattcaaat tagataaggg tttatctata aaataaaaaa
     2581 atcgtttcaa aattgaaacg attttttcta taaagtgtgt gttaatatta aggattaata
     2641 tctgttataa ccaccgccat ttccaccacg gtcacgatta taaccaccgc cgccaccgcc
     2701 acggttgcct ccaccaaagc caccactttt tctgaagccg ccgccaccgc cactgcttct
     2761 acgttcacct tcaggacgtg gagttgattc attaactaca atttttctgt cttgcacatc
     2821 agtatcattt aatgcactga ttgctgcacg tgctgcatca tcatcgttca tttcaacaaa
     2881 gccaaagcct ttgctgcgat tgtttttgaa tttgtctgta acaatttttg cagatgctac
     2941 ttcaccataa ggtgcaaata attcttgtaa atcttcactg gtcatattcc agtttaaatt
     3001 accaacgtaa atgttcattt cttgaattta taaatttaaa taaaaccaat aaaaacagta
     3061 accaaaaaac ctgaagcatt tacgttaaaa ccttccggaa atatggaaaa ccggtcattg
     3121 gaaaacacga aaatagtact tttatctaat taagcaagtt ttttttcaat cttttcttag
     3181 tagatagttt cagttataca ttttaaggtt aatatttaaa attgtattac gtaatgcagt
     3241 gttacatttt cattaatttc taattaaaac ttttacaacc cttattttta caaaaactat
     3301 tttttttgtt accaagtgtt gcaattgtta tattaaacta taatggaata aattatttac
     3361 aacagtttct tccttctgtt ctttcatcaa cttattctaa taaaaaaatt attgtagcag
     3421 ataatgcctc tacagatgat agtgttaact gggtgaagga aaattaccct caaattacag
     3481 ttctaataaa cactcaaaat aatggttatg ctggtggata caactggtgt cttaaaagtg
     3541 ttgaggcaga ttattttgta ctcttaaatt ctgatgttga agttactgct aattggatag
     3601 agccaataat agaattaatg caagcagata ctaaaattgc ggcttgccaa ccaaaaattt
     3661 tatcctggca tcacaaagaa aaatttgaat atgctggtgc ttgtggtggc tttatagatt
     3721 tatatggcta cccgttttgt agaggaagaa tttttgattt ttgtgaagat gataaagggc
     3781 aatataattc tgttgagaaa atattttggg catctggtgc agcattgttt attaagagca
     3841 aagtttttgt ggatttaaat ggatttgatg aaaacttttt tgctcacatg gaagaaattg
     3901 atctttgctg gagattacaa ttatctggtt atactattgc tgttcagcct gcatctattg
     3961 tttatcatgt aggtggtgga acattaccca aaagttatca gaaaactttt cttaatttta
     4021 gaaataattg gataatgctt tgtaagaatt taactattaa agaaaaattt ttaattttcc
     4081 cagttagatt ttttttagat tgtactactg cttgtaaagc tttattaagt aaagatacag
     4141 aaatggctag agccattttt aatgctcata ttgcttttat aaaatggaag ttttcttcaa
     4201 aatataaaag tgtacaacct aataaaccac tttataaact aaatggagtt ttattaaatt
     4261 ctat
//
LOCUS       DAOQHP010000181         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12383_length_4252_cov_3.764355, whole genome shotgun sequence.
ACCESSION   DAOQHP010000181 DAOQHP010000000
VERSION     DAOQHP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12383_length_4252_cov_3.764355"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PLY81_06610"
     CDS             complement(<1..1294)
                     /locus_tag="PLY81_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF29935.1"
                     /translation="MNQNNRFTDSGIEINKVYTKEDLPSSLTKEMPGDFPFTRGVQAD
                     MYRGKLWTMRQYAGFSTAEESNKRYHYLLSQGVMGLSVAFDLPTQIGYDSSHELAEGE
                     VGKVGVAIDSLEDMEQLFDGITLENVSTSMTINATGFILLAFYVALAKKQGADLKKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFEWCSKELPKWNTISISGYHIREAGST
                     AVQEIAFTLSNGKAYVKAALEKGLDINVFGKRLSFFFNAHNNLFEEVAKFRAARKMWA
                     TIMKDLGATDEKALMLRFHTQTGGATLTAQQPHNNICRVTIQTLAAVLGGTQSLHTNG
                     FDEALSLPTENAARIALRTQQIVAFESGVTDTVDPLAGSYFIESLTNEVEQKAWQLIQ
                     KIDAMGGSVSAIEEGFMQNEIAKSSYDYQCNIESNQKII"
     gene            1443..3293
                     /locus_tag="PLY81_06615"
     CDS             1443..3293
                     /locus_tag="PLY81_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNF29936.1"
                     /translation="MSTKEELLQDVVIKFAGDSGDGMQLTGQQFTNNTALLGIDLATF
                     PDFPAEIRAPIGTLPGVSGFQLHFSSERVFTPGDTCDVLVAMNAAALKVNLRSLKKGG
                     KIIANTDGFDTKNLRLANYKDGDNPLENGSLEGYELIKIDVTKITREALKDFAGMGTK
                     ERDRTKNMFVLGFVYWMYNRSLDSTIAFLKEKFGKNETILESNLKVLQAGYNYGDTTE
                     TFSSRYKVEKAKMPNGSYRSIMGNQALAYGLIAASNKSGLPMFLGTYPITPASDILHE
                     LSKHKNFGIKTFQAEDEIAGIASAIGASYGGQLGVTTTSGPGMALKTEAMGLAVMLEI
                     PLVICNVQRGGPSTGLPTKTEQSDLLQAYYGRNGECPMPVIASSTPSDCFETAFEAVR
                     IAVEHMTPVILLSDGYIANGAEPWRYPNANDLPNITVNFKKELDTNEEVFLPYLRNEK
                     LVRPWAVPGTAGLEHRIGGLEKQDKTGNVNYEPENHQLMIKLRQEKVDKVADFIPLQK
                     LDSGPEKGKVLVVGWGSTYGAIKSAVMTLQKQGHAVSHAHLRYVRPFPKNLAEILNNF
                     EKVLIPEINNGQLIKIIRDQYFIDAKGYNKIMGVPITKTELIEVISEMVK"
     gene            3343..4218
                     /locus_tag="PLY81_06620"
     CDS             3343..4218
                     /locus_tag="PLY81_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29937.1"
                     /translation="MKRTLKILTLLLTSFNLFGQTSYSGFIGKYPIELVTNIYFDGDA
                     RAIYAYSNHDEPIVINGELKQGKLTLYEKDNNGKSKATITFDNFDTKSNQLEGIWKEQ
                     NSIKQLKISLTKNFDIDFGENIEWTAKEIIQPVSLKDKYFKLVISKAKDNFYANVTGV
                     KIFEKKTDKLIQQIDLECELRGLNNISIGDYNFDGIEDFSVFEHSAAGPNTSSLYFLF
                     NPKKRKHFKSSFKGISLEFDPKTKRIYEHNQCCGGRSHLNAEYRVVNNQMVLVKMTCI
                     ENDEKIQDFKKVKCE"
BASE COUNT         1512 a          678 c          744 g         1318 t
ORIGIN      
        1 ctataatttt ttggttactt tcaatattac attggtaatc gtaggagctt tttgcaattt
       61 cattctgcat aaatccttct tcaatagcac ttacactgcc ccccatggca tctatttttt
      121 gaattaattg ccaagctttt tgttctacct cattagttag agattcaata aaataacttc
      181 ctgcaagtgg gtcaacggta tctgtaacac cactttcaaa agctactatt tgttgggttc
      241 ttaatgcaat tctagcagca ttttctgtag gtaaacttaa agcttcatca aaaccattgg
      301 tatgtaaact ctgtgtacca cctaaaacag cagcaagtgt ttgtatggta actctgcaaa
      361 tattattatg cggttgttgt gctgttaaag ttgccccacc agtttgtgta tgaaacctaa
      421 gcattaaagc cttttcatcg gttgcaccta aatctttcat tatagtagcc cacatttttc
      481 tggctgctct aaactttgca acttcttcaa ataaattgtt atgggcatta aaaaagaaag
      541 ataatctttt gccaaaaaca tttatatcta atcctttttc taatgcagct tttacataag
      601 cctttccatt acttaaggta aaagcaattt cttgcacagc agtgctgcct gcttctctaa
      661 tatgataacc tgatatagaa atagtattcc acttaggtaa ttctttactg caccattcaa
      721 atatatctgt tataatacgc attgatggct taggaggata aatataagtg cctctagcag
      781 catactcttt taaaatatcg ttttgtatgg tacccgaaat tttctttaaa tctgctcctt
      841 gtttttttgc taatgcaaca taaaatgcca ataaaataaa accagttgca ttaatagtca
      901 tagaggtgga tacattttct aaagtaatac catcaaatag ttgctccata tcttctaaac
      961 tatcaattgc tacaccaact ttaccaacct ccccttcagc caattcatgg ctgctatcat
     1021 acccaatttg agtaggtaaa tcaaatgcta cacttaaacc cattacacct tgagataaca
     1081 aatagtgata gcgtttattg ctttcttcgg ctgttgaaaa accagcatac tgcctcatcg
     1141 tccaaagttt tcctctgtac atatctgctt gcacccctct ggtaaaagga aaatcaccag
     1201 gcatttcttt ggtaagtgaa gaaggtaaat cttctttagt atatacttta tttatttcaa
     1261 taccactatc agtaaatctg ttattttggt tcatataaaa caatttgttt tacaactttt
     1321 tcagctaaat attttctatt tcagtaaaag ttcaaaggta aaagataaaa tagtaaaaaa
     1381 gtaacttcat ttaatcaata gcatatagct ttgtaacagt ttcaaatctt aaatattatt
     1441 ctatgagtac aaaggaagaa ttattgcaag atgtagtaat aaaatttgca ggagatagtg
     1501 gagatggtat gcagttaaca ggccagcagt ttactaataa tactgcatta ttaggaattg
     1561 acttagcaac tttcccagat tttcctgcag aaataagagc tcctattggt acactaccag
     1621 gtgttagtgg gtttcagtta cattttagta gcgaaagggt ttttactcct ggagatactt
     1681 gcgatgtact agttgctatg aatgctgcag ctttaaaagt aaacttaaga agcctaaaaa
     1741 aaggcggaaa gattatagct aatacagatg gctttgatac aaaaaattta agattagcca
     1801 attataaaga tggagacaac cctttagaaa atggaagttt agagggatat gaactgataa
     1861 aaatagatgt aaccaaaatt acaagagagg ccttaaaaga ttttgcaggc atgggtacaa
     1921 aagaaagaga tagaactaaa aatatgtttg ttcttggctt tgtatattgg atgtataata
     1981 gaagtttaga tagtaccatt gcctttttga aagaaaagtt tggaaaaaat gaaaccattt
     2041 tagaaagtaa cctaaaagta ttacaagcag gctacaatta tggcgatact acagaaactt
     2101 tttcatcaag gtataaagtg gaaaaagcta aaatgccaaa cggttcttac agaagcatta
     2161 tgggcaacca agcactggct tatggcttaa tcgcagctag taataaaagt gggttaccaa
     2221 tgtttttagg cacctaccct attactcctg cttcagatat tttacatgaa ctaagcaaac
     2281 acaaaaattt tggtattaaa acttttcagg cagaagatga aatagcaggt attgcaagtg
     2341 caataggtgc aagttatggt ggacaattag gtgtaacaac aacatctggt cctggtatgg
     2401 cattaaaaac agaagcaatg ggcttggctg ttatgctcga aattccgtta gtaatttgta
     2461 atgtgcaacg tggtgggcct agtactgggt taccaacaaa aacagagcaa agcgatttgt
     2521 tacaagctta ttatggcaga aatggcgaat gcccaatgcc tgtcatagca agtagtacgc
     2581 ctagcgattg ttttgaaaca gcttttgaag cagttagaat tgcagttgaa cacatgactc
     2641 ctgttatttt attaagcgat ggatatatag caaatggagc agagccttgg cgttacccaa
     2701 atgccaacga tttaccaaat attactgtaa attttaaaaa agaattagat actaacgaag
     2761 aagttttttt accctattta agaaacgaaa aactagtacg tccttgggct gttcctggta
     2821 ctgctggttt agagcataga attggtggct tagaaaaaca agataaaact ggcaatgtaa
     2881 attatgagcc agaaaatcac caattaatga ttaagttaag acaagaaaaa gtggataaag
     2941 tagccgactt tattcctttg caaaaattag atagtggacc agaaaaaggt aaagttttag
     3001 tggttggttg gggcagtact tatggggcaa ttaaaagtgc agtgatgact ttacaaaagc
     3061 aaggccatgc agttagccat gcacatttac gctatgttcg tccttttcct aaaaacttag
     3121 ctgaaatttt aaacaatttt gaaaaagttt taattccaga aataaacaat gggcaattga
     3181 taaaaattat tagagaccaa tattttatag atgctaaagg atataataaa ataatgggtg
     3241 tgccaattac taaaactgaa ctaatagagg taattagtga aatggtaaaa tgattattcg
     3301 tttattgtct tagttgccac ctattttagg acaaaacaca aaatgaaacg aacacttaaa
     3361 atattgacac ttcttctgac ttcgtttaac ttatttggac agacaagtta ttctggattt
     3421 attggcaaat atccaattga attagtaaca aacatatact ttgatggaga tgcaagagca
     3481 atttatgcat actctaacca tgacgaacct attgtaatta atggtgaact taaacaaggt
     3541 aagttgacac tttacgaaaa agacaataat ggaaagagca aagctacaat aacctttgac
     3601 aattttgaca caaaaagcaa tcaattagaa ggaatttgga aagaacaaaa ttcaatcaag
     3661 caactcaaaa ttagtttgac aaaaaatttt gacattgact tcggtgaaaa cattgagtgg
     3721 acagcaaaag aaattataca gcctgtttct ttaaaagaca agtatttcaa attggtaatt
     3781 tctaaggcaa aagacaattt ttatgcaaat gtgactggcg taaaaatatt cgagaaaaag
     3841 acagataagc taattcagca aattgattta gaatgtgaac ttaggggact aaacaatata
     3901 agtatcggag attacaattt tgatggcatt gaggactttt ctgtatttga acatagtgca
     3961 gctggaccaa acacctcaag tttatatttt cttttcaacc ctaagaaaag aaaacatttt
     4021 aagagttcat ttaaaggaat ttcattggag tttgacccta agacaaaacg tatttacgaa
     4081 cataatcaat gttgtggagg acgaagccat ttgaacgcag aatacagagt tgtaaacaac
     4141 caaatggtat tagtgaagat gacttgtata gagaatgatg aaaaaataca agattttaaa
     4201 aaagtaaaat gcgaataaaa acagcctata acattgagtt tctccaatcc gt
//
LOCUS       DAOQHP010000182         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12416_length_4246_cov_3.654021, whole genome shotgun sequence.
ACCESSION   DAOQHP010000182 DAOQHP010000000
VERSION     DAOQHP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12416_length_4246_cov_3.654021"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1268)
                     /locus_tag="PLY81_06625"
     CDS             complement(123..1268)
                     /locus_tag="PLY81_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF29938.1"
                     /translation="MKNRVEEIRTFIEYKDFDLARRRLLDACLDTRKESLIQQSINFS
                     KQVGNKAEQNLANFSDKFQDGAEQILQQFSSLSSSVIHTNEQVISANKISKSYTVGGF
                     NLQALSLKAYTNKIIGVVGENGHGKTTLLRCLAGQLQINEGSIDYYFDPKPDYFSIKQ
                     KVAFIPQRIPKWYGKLKDNLHFSASITGNYSKENNLMVDFMLERLSLTSYANLTWSQI
                     SSGYRTRFEIARVLLQKPTLLILDEPLANLDINAQQTMLIDFKYLAKSIYYPLSIILS
                     SQQLHEVEKVADEILFIKNGNCIFQTTCSNNVTHKEVVIEIETNANRDTILQILENNV
                     QVSYNGGYYTITSNQLTANVIMEKLLAQQIAITYFRDITNSTKRLFS"
     gene            complement(1281..1739)
                     /locus_tag="PLY81_06630"
     CDS             complement(1281..1739)
                     /locus_tag="PLY81_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29939.1"
                     /translation="MKGLFFVLLIVSNTAFSQQRVKDSTEVANTFNTILKECKNVNFA
                     DPDIAKYGTFYKVAPYIIYQGKDEKRKWKDYCNYSVEEEKNGVDEICFRINRTINKAD
                     SYKIVQYKTEKESEGLWHILHVSLLLNGKQKQMSFAFLKPEKKFALGDID"
     gene            1886..3268
                     /locus_tag="PLY81_06635"
     CDS             1886..3268
                     /locus_tag="PLY81_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF29940.1"
                     /translation="MLRRKFLQQTSILTIGSIAAPQIIFGAQKLEIKKIRVGLIGVGL
                     RGQNHLDVLLRREDVLVTALADPQPKMLAMANELIAKSGKPKPLIFSNGNYDYKNLLK
                     RDDVDAVIISTPWEWHAEQTIDSLLAGKIPGVEVSGAVKLAECWDIVNAAEKTGLPVM
                     VLENVCYRRDVMAVLNMVRKGMFGELLHAQGGYQHDLRGVKFNDGITPYNSGVEFGEK
                     SFSEAAWRTQHSITRNGELYPTHGLGPVAVMMDINRGNRLTKLSSIATKAKGLHRYIV
                     NHPKGGENHPNAKIKFKLGDIVTTQLQTANGETIVLTHDTNSPRPYNMGFRVQGTNGI
                     WQDNHAGEFTAGLIYFEDKSKPHRWENTEQNMKTYDHPLWKKYEKDTAGAGHGGMDFF
                     VINAFIECIKQKTEFPLDVYDLATWYAITPLSEKSIEGGGKLMNIPDYTRGKWKTRKP
                     VFCIKEDGNY"
     gene            complement(3246..>4246)
                     /locus_tag="PLY81_06640"
     CDS             complement(3246..>4246)
                     /locus_tag="PLY81_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNF29941.1"
                     /translation="WSNIEYNFISSGLINNTAKITAKQGSFILQQINTGVFATPSTID
                     NNIQLLKQFVQINNPDYLFTNTIETKQKETLICINNNYYRAFNFIENSYTIESVTHES
                     EAYEAATAFANFTNVFSNFNSDSLQDTIPHFHNLELRFSQFEKALKIGNQQKIKEHRI
                     IIDEIYSVENICKKYNALLGSKECKKRVTHHDTKISNVLFKQNKAICVIDLDTVMSGY
                     FISDVGDMFRTYLSPVTEEETNLDAICIRKEVLEAIYQGYMDYMHQHLSKYEKQFFYF
                     AGEVMIYMQAIRFITDFLMNDIYYKTSYYHQNLNRAKNQLTLLKAFQSATNNCHLP"
BASE COUNT         1446 a          633 c          662 g         1505 t
ORIGIN      
        1 tgtaccaaat tacccaatga attttggtgc tccatattgt tggattattt tttaataacc
       61 tctcgtctaa ttttagtaaa aatgaaggct taaacttttg tatatctttc attacttgca
      121 ttttaactga acaatctttt tgtagagtta gtaatatctc taaaataagt aatggcaatt
      181 tgctgtgcta acaacttctc cataataaca ttggcagtta attgattaga agttatagta
      241 taataaccac cattataact tacctgaaca ttattttcaa gaatttgtaa aatagtatct
      301 ctgttagcat tggtttctat ttcaatcacc acttctttgt gtgtaacatt gttgctgcat
      361 gtagtttgaa atatgcagtt accattttta ataaataata tctcatctgc tactttttca
      421 acttcgtgta attgttggct acttaatata atgcttaaag gataataaat agatttagct
      481 aaatatttaa aatctattag catggtttgt tgtgcattaa tatcaaggtt ggccagtggc
      541 tcatctaaaa taagtaaggt aggtttttga agtaatactc ttgcaatttc aaaccttgtt
      601 ctataaccac tactaatttg gctccaagta aggttggcat agcttgttag cgataaacgc
      661 tctaacataa aatcaaccat taaattattt tctttgctat aattacctgt aatagatgca
      721 gaaaaatgta gattatcttt tagttttcca taccatttag gaatgcgttg gggtataaaa
      781 gctacttttt gttttatgga aaaataatct ggcttaggat caaagtaata atcaattgag
      841 ccttcattta tttgtaactg cccagctaaa caacgcaata aagttgtttt gccatgacca
      901 ttttcgccaa ccactccaat aattttattg gtatatgctt ttaatgaaag tgcttgtaaa
      961 ttaaacccac ctacagtgta cgatttgctt attttatttg cagaaattac ttgctcatta
     1021 gtatgaatta ctgaagaact tagacttgaa aattgttgta aaatttgttc tgcaccatct
     1081 tgaaatttat ctgaaaaatt ggctaaattt tgctctgctt tattaccaac ctgcttacta
     1141 aagttaattg attgctgtat taaactctcc tttctggtat ctaaacaggc atctaataaa
     1201 cgtcttcttg ctaaatcaaa atctttatac tcaataaaag ttcttatttc ttctactcta
     1261 tttttcatag cggtacattt ttaatcaata tcgcccaaag caaacttttt ttctggtttt
     1321 aaaaaggcaa aagacatttg tttttgttta ccatttaaaa gcaaggaaac atgcaaaata
     1381 tgccataagc cttccgattc tttttcagtt ttatattgta caattttata actatctgcc
     1441 ttgtttattg ttctatttat tctaaaacaa atttcatcca ctccattttt ttcttcctca
     1501 acagagtaat tacaataatc cttccatttt cttttttcat ctttcccttg ataaattata
     1561 tagggtgcca ctttatagaa tgtgccatat tttgcaatat cagggtctgc aaaatttaca
     1621 tttttacatt cctttaaaat tgtattaaat gtgtttgcca cttcggtaga gtctttaact
     1681 ctttgttgag agaatgctgt attagaaact atcaataaca caaaaaataa acctttcaca
     1741 atagataatt tttttagtta aataattaga tgctagaaat tgagaattgc acaatctata
     1801 aaattagaca atttaaaaat aatacatttg ctaataaata attattttac atctcacata
     1861 attaatacac caatttatga aacctatgtt aagaagaaag ttcttacaac aaacaagtat
     1921 attaactatt ggcagcattg ctgcaccaca aataattttt ggtgctcaaa aattagaaat
     1981 taaaaaaata agggttggct taattggggt tggcttaaga ggtcaaaatc atttagatgt
     2041 tttattgaga agagaggatg tattggtaac agcattggcc gaccctcaac caaaaatgct
     2101 tgcaatggcc aatgaattaa ttgctaaaag tggcaaacca aaacctttaa tttttagtaa
     2161 tggtaattac gattataaaa atttattgaa aagagatgat gtagatgcag taattatttc
     2221 aacaccttgg gaatggcatg cagagcaaac aatagatagc ttattagctg gaaaaatacc
     2281 tggagtggaa gtatctgggg ctgttaagtt agctgaatgt tgggatattg taaatgctgc
     2341 agaaaaaacc ggattgcctg taatggtttt agaaaatgtt tgttatagaa gagatgtgat
     2401 ggctgtttta aatatggtta gaaaaggaat gtttggcgag ttgttgcatg cacaaggagg
     2461 ctaccaacat gatttaagag gtgtgaaatt taatgatgga attactccgt ataactctgg
     2521 tgtagaattt ggagaaaaaa gctttagtga agctgcttgg agaacacaac atagcataac
     2581 cagaaatgga gaactttacc caacacatgg tttaggacct gtagcagtaa tgatggacat
     2641 taatagagga aataggctta ccaaactttc ctctatagct acaaaagcca aaggcttgca
     2701 tcgttacatt gtaaaccacc ctaagggtgg cgaaaaccat cctaatgcaa aaattaaatt
     2761 taaattagga gatattgtaa caacacaatt gcaaactgca aatggcgaaa ccatagtttt
     2821 aacacacgat acaaattctc cacgccccta caacatgggt tttagagttc aaggcaccaa
     2881 tggtatttgg caagataacc atgcaggtga gtttactgca ggcttaattt attttgaaga
     2941 taaaagcaaa ccacatagat gggaaaatac agaacaaaat atgaaaacat acgatcaccc
     3001 attatggaaa aaatatgaaa aagatactgc aggtgccgga catggtggaa tggatttttt
     3061 tgtaataaat gcttttattg aatgtatcaa acaaaaaact gaatttccac ttgatgttta
     3121 tgatttagct acttggtatg ctataacccc tctatctgaa aaatctattg aaggtggagg
     3181 aaaactaatg aatataccag attatacaag aggtaaatgg aaaacaagaa agccagtttt
     3241 ttgtattaag gaagatggca attattagta gcagactgaa atgcttttaa taaggttaat
     3301 tgatttttag ctctgtttaa attttgatgg tagtagcttg ttttgtaata aatatcgttc
     3361 attaaaaaat cagttataaa ccttattgct tgcatgtaaa tcataacttc gcctgcaaaa
     3421 taaaaaaatt gtttttcgta ttttgaaaga tgctggtgca tgtaatccat atatccttga
     3481 tatattgcct ctagtacttc ttttcttata caaatggcat ccaaattagt ttcttcttct
     3541 gttacagggc ttaaataggt tctaaacatg tctcctacat cactaataaa ataaccagac
     3601 attacagtat ctaaatcaat aacgcaaata gctttatttt gtttaaacaa tacattgcta
     3661 atttttgtat cgtggtgtgt aactcttttt ttacattctt tagaacctaa tagagcattg
     3721 tattttttac aaatattctc tacactatat atttcatcaa taataattct atgttcttta
     3781 attttttgtt gattgccaat tttcaatgct ttttcaaact gcgaaaacct tagctctaaa
     3841 ttatggaaat ggggtatggt atcttgtaag ctatcagaat taaaattaga aaacacattg
     3901 gtaaaatttg caaatgcagt tgcagcttca taagcttcac tttcatgagt aacactttct
     3961 attgtgtaag aattttcaat gaaattaaaa gctctgtagt aattattatt tatgcaaatt
     4021 aaagtttctt tctgcttagt ttcaatggta ttcgtaaata aataatctgg attatttatt
     4081 tgtacaaatt gtttaagtaa ttgtatatta ttatcaatag tagatggagt agcaaaaacg
     4141 cctgtgttta tttgttgcag aataaaactt ccttgcttgg ctgtaatttt agctgtatta
     4201 tttattaagc cagatgaaat gaaattatac tcaatattag accagc
//
LOCUS       DAOQHP010000183         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12466_length_4236_cov_3.474767, whole genome shotgun sequence.
ACCESSION   DAOQHP010000183 DAOQHP010000000
VERSION     DAOQHP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12466_length_4236_cov_3.474767"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLY81_06645"
     CDS             <1..316
                     /locus_tag="PLY81_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29942.1"
                     /translation="LLSFFRYVVFVVQYLLLLQLFNVEVVWYKLAWMVCVLFLVLAVI
                     PSIPIAELGLRGETSKQLFGLLSSNIIGIVFTAAIIWIINRVIPAIAGSLFILGVRLF
                     KK"
     gene            322..1119
                     /locus_tag="PLY81_06650"
     CDS             322..1119
                     /locus_tag="PLY81_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNF29943.1"
                     /translation="MKKKVLSLFVAVGSWLTLTSADDDFCNIRNTTFVDGENITYKIF
                     YNALGIYLDAGTASFTVTKETLNNTPVFHIVGLGTSNSSYDWIFKVRDRYESFIDTTT
                     LLPIKFIRNVDEGGYKFYENVTFNQQTNSAITNKGVYKVPNCVQDVLSSIYYSRNIDF
                     NKYKIGDKIPFTMFLDNEIHNLYIKYYGKETIKTRYGKFKAIKFKPLLIKGTIFEGGE
                     KMTVWVSDDANHIPLRIESPIAVGKIKVDMMMYKNLRNSLSSLIKFR"
     gene            complement(1125..1814)
                     /locus_tag="PLY81_06655"
     CDS             complement(1125..1814)
                     /locus_tag="PLY81_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29944.1"
                     /translation="MRIKINGMRLLFLFFILLFINNNLNAQCATFKLARNGDTLNCVD
                     KAGNKRGKWIIKVDPLRGNPGYEEEGIFADNRKEGIWRRYNLMGDLIAIQSYKWGNLD
                     GVSQYFTISGIEREESWKAMNPLNAYDTFLVEDINDENKYQQVILPNDGKSLKNGRWT
                     WYRPGGMGIIKTELWSLNKLVVPKTETQNTNEEEIKKPEQPKPKPKEVNDYEKKNKNK
                     KSVKVRDGKTG"
     gene            complement(1878..2588)
                     /locus_tag="PLY81_06660"
     CDS             complement(1878..2588)
                     /locus_tag="PLY81_06660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF29945.1"
                     /translation="MVKYEIIEENDFFIAINKPSGLLSVPDRKQSEPSLKDFLLQKYS
                     SIYTVHRLDKDTSGVIVFAKDADTHKQLSLLFEGRDVEKFYLGLVHGSLLHHNGSIDA
                     PIMMHPANNATMIIHQKGKPSYTDYTLLEQFKNYSWVQFQIHTGRTHQIRIHSQYMGN
                     SIVCDAIYGDDKPILLSSLKKKFKLSKKEEEEKPLLNRLALHSALLKFTFNNQAYHIE
                     APLPKDLRALLEQLRKNN"
     gene            complement(2591..3652)
                     /locus_tag="PLY81_06665"
     CDS             complement(2591..3652)
                     /locus_tag="PLY81_06665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF29946.1"
                     /translation="MKKNGFDKFINKEPKAAAKKEAFRQEKRRAKAAANALGEEMRRK
                     KQDALNNKLDVKDKFSTAKKNIKPTNKKQPTTYNKFSTTNKKDIGSKVQNTTSKYQPS
                     ANMPLNKFIAHAGICGRREAAELVKKGEITVNNEIIFEPGFKVNGDETIKFKNKRIFI
                     QQNLVYILLNKPKDYITTVKDPQGRKTVFDLIKNAAPERVYPVGRLDRNTTGVLLLTN
                     DGDLAQKLSHPSYQIKKIYEVKLDKPVLKKDMEAIANGITLEDGFIQADSIAYADDKD
                     KSIIGIEIHSGKNRIVRRIFESLGYDVRNLDRVMFANLTKKNVDRGKWRMLSEKEIRL
                     LKFMNKSFMKNNKSKASAE"
     gene            complement(3744..>4236)
                     /locus_tag="PLY81_06670"
     CDS             complement(3744..>4236)
                     /locus_tag="PLY81_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNF29947.1"
                     /translation="FQRLGIKLERRGDDIYIPAHTNGYEIKTDIDGSILTVADAPWPG
                     FTPDLLSIVLVVATQAIGSVLVHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLG
                     RRNKLRGITMSSPDIRAGQALLIAALSAEGKSIIQNIEQIDRGYQFIDERLRSIGANI
                     IRK"
BASE COUNT         1289 a          704 c          591 g         1652 t
ORIGIN      
        1 actactttct ttttttagat acgttgtatt tgttgtgcag tatttattgt tactacaatt
       61 gtttaatgta gaagttgttt ggtataagtt agcttggatg gtttgtgtat tatttttagt
      121 acttgctgtt attccatcta taccaattgc agagttagga ttaagaggcg aaacaagtaa
      181 acagttattt gggttgttaa gtagcaatat tattggcata gtttttactg ctgccattat
      241 atggataatc aacagagtta ttcctgctat tgcaggaagc ttgtttatat taggtgtacg
      301 actatttaag aaataagcaa tatgaaaaaa aaagtattat ctctttttgt tgccgtgggc
      361 tcttggctta cattaacatc tgccgatgat gatttttgta atattagaaa taccactttt
      421 gtagatggtg aaaatattac ctataaaata ttttacaatg ccttaggtat ttatttagat
      481 gctggtactg caagttttac agtaactaaa gaaactttaa acaacactcc tgtttttcat
      541 attgtaggtt taggtacttc aaactctagc tacgattgga tttttaaagt aagagaccga
      601 tatgaatctt ttattgatac cactacctta ttacctataa aatttattag aaatgtagat
      661 gaaggtggat acaagtttta cgaaaatgtt acatttaatc aacaaacaaa ttcggcaatt
      721 accaataaag gtgtatataa agtaccaaat tgtgtacaag atgttttaag ctcaatttat
      781 tattctagaa acatagattt taataaatat aaaattggcg ataaaatacc atttaccatg
      841 tttttagata atgaaataca caacctttat ataaaatatt atggtaaaga aactatcaaa
      901 acccgatatg gaaagtttaa agcaataaaa tttaagccat tattaattaa aggaaccata
      961 tttgaaggag gagaaaaaat gactgtttgg gtaagcgatg atgctaacca tataccttta
     1021 agaatagaaa gccctattgc tgttggtaaa attaaagtgg atatgatgat gtataaaaac
     1081 ttacgtaatt ctttaagctc cctaattaag tttagataag aatattaacc agtttttccg
     1141 tctctaacct taaccgattt tttgttcttg tttttctttt cgtagtcgtt tacttcttta
     1201 ggttttggtt taggctgttc aggttttttt atttcttcct cgttagtatt ttgtgtttct
     1261 gttttaggaa ccactaactt atttagcgac cacaattctg ttttaataat acccatgcct
     1321 cctggtctat accatgtcca gcggccattt tttaaacttt taccatcatt gggtaatatt
     1381 acttgctggt atttattttc atcatttata tcttctacta aaaaagtatc ataagcattt
     1441 aatggattca ttgccttcca gctttcttct ctttctatac ctgaaatagt aaaatattgc
     1501 gaaaccccat ctaaattccc ccatttgtaa ctttgaattg caatcaaatc acccatcaaa
     1561 ttatatcttc gccaaatgcc ttcttttcta ttgtctgcaa aaattccttc ttcttcataa
     1621 cctgggttac ctcttaatgg gtccacttta ataatccatt ttcctctttt attgcctgcc
     1681 ttatctacac aatttaaagt gtctccattt ctggcaagct taaaagtagc acattgagca
     1741 ttaaggttat tatttataaa caataagata aaaaataaga atagcaaacg cataccatta
     1801 attttaattc tcaaaaataa actatattaa tattaaaaaa gttataaaat attctaccac
     1861 ctatatacaa acatttttta attatttttt cgtagctgtt ctagcaaagc tcttaaatct
     1921 tttggcaatg gtgcttctat atgataggct tgattattaa aggtaaactt taataatgct
     1981 gaatgtaaag ccaacctatt taataatggc ttctcctcct cttctttttt agaaagttta
     2041 aatttctttt ttaaagaaga aagtaaaata ggtttatcat ctccgtaaat agcatcacaa
     2101 actatgctat tgcccatgta ttgagagtga attcttattt gatgagttct tcctgtatga
     2161 atttgaaatt gaacccatga gtagttctta aactgttcta ataaagtata atctgtataa
     2221 gagggctttc ctttttggtg aataatcata gttgcattat tagcaggatg catcataatg
     2281 ggagcatcaa tactaccatt atgatgtaac aaactaccat gtaccaagcc aagataaaat
     2341 ttttcaacat ctctgccttc aaatagcaga gatagttgtt tgtgggtatc tgcatcttta
     2401 gcaaaaacaa ttactccact agtgtcttta tctaacctat gtactgtata aattgaagaa
     2461 tatttctgta gcaaaaaatc ctttaaacta ggttccgatt gttttctatc tggtacgctt
     2521 aataagcctg atggtttatt tatagcaata aaaaaatcgt tctcttctat tatttcatat
     2581 ttaaccatag ctactctgca gatgctttgc ttttattgtt tttcataaaa cttttattca
     2641 taaacttcag taaccttatt tctttttcac taagcatacg ccacttgcct ctatctacat
     2701 tttttttagt taggttggca aacataactc tatctaaatt tcttacatca tagcctaaac
     2761 tctcaaaaat tcttcttaca attctatttt tgccgctatg tatttcaatt ccaataatgc
     2821 ttttatcttt atcatcggca taagcaatac tatctgcttg tataaagcca tcttctagtg
     2881 taataccatt tgcaatagct tccatatctt ttttaagtac aggcttatca agctttacct
     2941 cataaatttt ctttatttgg taagatgggt ggctaagttt ttgtgctaaa tccccatcgt
     3001 tagtaagtaa taaaacacca gttgtatttc tgtctaatct gcccacagga tatacccttt
     3061 ctggggcagc attttttatt aaatcaaaaa ccgtttttct gccttgtggg tccttaacag
     3121 tagtaatata atcttttggt ttattgagta aaatatatac aagattctgc tgtataaaaa
     3181 tacgtttgtt tttaaatttt atagtttcat ctccatttac tttaaagcct ggctcaaaaa
     3241 ttatttcatt gtttacagta atttctcctt tttttactaa ctctgcagct tctcttcttc
     3301 cgcaaatacc tgcatgagca ataaatttat ttaaaggcat attggcagat ggctgatatt
     3361 ttgaagtagt gttttgaact ttgctgccta tgtctttttt attggttgta gaaaatttgt
     3421 tgtaagttgt tggttgtttt ttattggttg gctttatatt cttttttgct gtagagaatt
     3481 tatcttttac atccaactta ttatttaatg catcttgttt tttccttctc atctcttctc
     3541 ctaatgcatt ggctgctgct tttgctcttc ttttttcttg acgaaatgcc tcttttttag
     3601 cggcagcttt aggttcctta ttgataaatt tatcaaaacc attttttttc atactataca
     3661 attttactgt tttacaacag gatgattaat gttgcaaaga tatttattag tattattata
     3721 tgcttaatta aaaagtgtag tagttatttt ctaataatat tagctccaat gcttctcaat
     3781 ctttcatcaa taaattggta tcctctatct atttgttcta tgttttgaat aatacttttt
     3841 ccctctgcac ttaaagctgc aattagtaat gcttgtcctg ctctaatatc tgggctgctc
     3901 attgttatac cacgcagttt atttcttctt cctaagccaa taactgtagc tcggtgtgga
     3961 tcgcataaaa taatttgtgc ccccatatca attaatttat ctacaaaaaa caatctgctt
     4021 tcaaacattt tttggtgtac caatacacta cctattgctt gtgtggcaac tactaaaaca
     4081 atactcaata aatctggtgt aaatcctggc caaggtgcat ctgcaaccgt aagtatagac
     4141 ccatcaatat cagtttttat ttcataacca tttgtatgag caggaatata aatatcatca
     4201 cctcttcttt cgagcttaat tcccaagcgt tgaaaa
//
LOCUS       DAOQHP010000184         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12468_length_4235_cov_3.833254, whole genome shotgun sequence.
ACCESSION   DAOQHP010000184 DAOQHP010000000
VERSION     DAOQHP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12468_length_4235_cov_3.833254"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="rpmA"
                     /locus_tag="PLY81_06675"
     CDS             complement(<1..207)
                     /gene="rpmA"
                     /locus_tag="PLY81_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNF29948.1"
                     /translation="MAHKKGEGSVKNGRDSNSKRLGVKIYGGQPATAGNIIVRQRGTT
                     YHPGKNIGVGKDFTLFALADGIVEF"
     gene            complement(282..587)
                     /gene="rplU"
                     /locus_tag="PLY81_06680"
     CDS             complement(282..587)
                     /gene="rplU"
                     /locus_tag="PLY81_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNF29949.1"
                     /translation="MLAVVKIAGQQFKVQAGDSLYVPHLQGSTGDKVEFNEVLLVDNA
                     GTVSAGSNAKATVKAEIIKSLVQGDKVIAFKMKRRKGFRKKHGHRTHYTQIKIESIA"
     gene            complement(726..1034)
                     /locus_tag="PLY81_06685"
     CDS             complement(726..1034)
                     /locus_tag="PLY81_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF29950.1"
                     /translation="MQNITVEELKERLDTGNPINLIDVREETEHADFNIGGINFTLRR
                     MQDMAIEDIEHLKNEEVICYCRSGQRSNMACLILEYQGFTNTINVIGGVNAWQEKFGR
                     "
     gene            complement(1049..1867)
                     /locus_tag="PLY81_06690"
     CDS             complement(1049..1867)
                     /locus_tag="PLY81_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNF29951.1"
                     /translation="MKLFKFITDKPLWVNIVVAITLLMVLIFIFFISLKSITSHGESI
                     KVPNTLGQNITAAQKMLESQGFSVEVLDSVFIDTLARLSVFKQSPEADVVVKKGRTIY
                     LTVNKAIAPEVEMPSLIGYSYKSAELMLQSLSLKMGDTSYKADFARNSVLEQYYNRKP
                     IKEGTKIPMGSTISFVLGSGVGNTDINVPNLLGLKVSEALGILQNLHLNVGPIIAQEA
                     ISDTLNAFVTEQRPPAFSEPTPGQKVQNKLRAGQVIDLFIRNTPITVQDSTITQ"
     gene            complement(1928..2911)
                     /locus_tag="PLY81_06695"
     CDS             complement(1928..2911)
                     /locus_tag="PLY81_06695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNF29952.1"
                     /translation="MKKCIAIVTGGYSKEAEISYKSVITVTNNIDKQKFDWYIIDINP
                     EGWWYLNDANEKIFVNKNNFTIDVNNTTITFDAVLMCMHGTPGEDGKLQGYFDMIGLP
                     YTSCNAAVSALTFNKKFTVSVAANSGINVAKSIMLFKNNSINEKEIAQQLKFPVFVKP
                     NNGGSSIGMSKVYETSNLSAAISKAFEEDDQILIEEFIAGRELTIGVFKTKGEIITLP
                     ITEIITKNDYFDYKAKYLGESDEITPANVEETIAEKIRNAAKKAYQVFNCNGIVRIDF
                     IFESKLQQPFMLEINTVPGQSAESIVPQQVKAMGWSLQDFYTALIEECFTK"
     gene            3212..3829
                     /locus_tag="PLY81_06700"
     CDS             3212..3829
                     /locus_tag="PLY81_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNF29953.1"
                     /translation="MQWHKKNTIFLLIFSLFLFFSKEIKAQGKYDTIKVYAYITAYGD
                     TIPMSFLPPVEVSTKLSRAARRHLTEWNRLRKAVYVTYPYAIAASRIMNEVNAKLVGV
                     KNKKQRKKIIRAREKELKKEFADKLTQMSVYQGKVLMKLINRQTGNNCYEIIDEYKGS
                     FTAAFYQTIAIIFGSNLKQPYDAKGKDADMEKIVIDVEKMYGYRS"
     gene            3844..>4235
                     /locus_tag="PLY81_06705"
     CDS             3844..>4235
                     /locus_tag="PLY81_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF29954.1"
                     /translation="MTPERTEKLLNVLSRRQHNLTVVMENVQDPHNISAVMRTCDAVG
                     IQDIYILNTKIPRHKKFGAKSSSSAAKWLTVHQFDNVQDCFNELRTKYNKIFTTHLST
                     GAVSLYEINFTENIALVFGNEHSGVSEEV"
BASE COUNT         1401 a          662 c          590 g         1582 t
ORIGIN      
        1 aaattcaact attccgtctg ctaatgcaaa taatgtaaag tcttttccta caccaatatt
       61 tttaccagga tgataagttg ttcctctttg acgaacaatt atattacctg ctgttgctgg
      121 ttgaccacca tatattttta cacccaaacg tttgctgtta gaatcacgtc cgttttttac
      181 actaccttcc ccttttttgt gtgccataat tttatttttt aaaaactaat tactttataa
      241 aattaaaaag cattttattt tttgctttta gttttatttt tttaagctat actttcaatt
      301 ttaatttgcg tatagtgagt tctatgacca tgcttttttc taaagccttt tcttcttttc
      361 attttaaaag caattacttt atcgccctga actaaagatt taattatttc ggcttttact
      421 gttgctttgg cattactacc tgcagaaaca gttcctgcat tatctactaa taatacttca
      481 ttaaattcta ctttatctcc agtgcttcct tgtaaatgcg gtacatataa actgtctcca
      541 gcttgcactt taaattgttg acctgcaatt tttactactg ctaacatgac tttttaaaaa
      601 ttaggacggc aaaagtaaga atatttgctc aatttcagca aactttttgc atattttatt
      661 tttcaaggct aattattaag gaaataacct gaaaataaat ggtttagggt gctataattc
      721 aagccttatc ttccaaattt ttcttgccag gcgtttactc cgcctataac attaatggta
      781 tttgtaaaac cctggtactc taaaattaag catgccatat tgcttctttg tccacttcta
      841 caataacaaa ttacttcttc attttttaaa tgttcaatat cttctattgc catgtcttgc
      901 attcttctta aagtaaaatt aatacctcct atattaaaat cggcatgttc tgtttcttct
      961 ctaacatcta ttaaattaat agggttacca gtatctaatc tttctttaag ctcttctact
     1021 gtaatgtttt gcataaaata aatttatatt attgtgtaat ggttgagtct tgaacagtta
     1081 ttggtgtatt tctaataaat aaatctataa cctgtccggc tcttaatttg ttttgaactt
     1141 tctgaccagg tgtaggttcg ctaaaggcag gaggcctttg ctctgttaca aatgcattta
     1201 acgtatcaga tattgcctct tgtgcaatta ttggtcctac gtttaaatgt aaattttgca
     1261 aaatacccaa tgcttcagaa acttttaagc caagtaaatt aggcacatta atatcagtat
     1321 taccaacacc acttcctaat acaaaactta tggtactgcc cattggtatt tttgttcctt
     1381 ctttaattgg cttacgattg taatattgtt ctagcactga atttcttgca aagtctgcct
     1441 tgtaactggt atcacccatt tttaaactta aactttgcaa cattagctct gcacttttat
     1501 aagagtatcc aattagcgaa ggcatttcaa cttcgggtgc aattgcttta ttaactgtta
     1561 agtaaatggt tcttcctttt ttaacaacta catctgcttc tggtgattgt ttaaaaacgg
     1621 ataaacgagc taaagtgtca ataaaaacag agtctaacac ttctacacta aaaccctggc
     1681 tctctaacat tttttgtgca gcggtgatat tttgccccaa tgtattgggt actttaatag
     1741 attcaccatg actggtaatt gattttaagg aaatgaaaaa tataaaaatt aataccatta
     1801 acaaagttat tgcaacaaca atatttaccc aaagtggttt atcggttata aacttaaata
     1861 atttcattta aataaaattc aatacaaaaa tgtagaaaaa ttatgatgtt gattgtaata
     1921 aattgaatta tttggtaaaa cattcctcta tcaatgcagt ataaaaatct tgtaaactcc
     1981 agcccattgc tttaacctgc tgtggtacta tactttctgc actttggcct ggtactgtat
     2041 taatttctaa cataaaaggt tgttgtaatt tgctttcaaa aataaaatca atacgtacta
     2101 tgccattgca attaaatact tggtaagctt ttttggctgc atttcttatt ttctctgcaa
     2161 tagtttcttc tacattggct ggtgtaattt catcactttc tcctaaatat tttgctttat
     2221 aatcaaagta gtcattcttt gtaattattt cggtaattgg aagagttata atctcacctt
     2281 ttgttttaaa cacaccaatt gttaattctc tgccagcaat aaattcttca attaaaatct
     2341 gatcatcttc ttcaaaagct ttgctaatag cagcagatag gttacttgtt tcatatactt
     2401 tgctcatgcc tatactactg cccccattat taggttttac aaaaaccgga aattttagtt
     2461 gctgagcaat ttctttctcg tttatagaat tatttttaaa taacatgata gacttcgcaa
     2521 catttattcc actatttgct gcaacagata ctgtaaattt tttattaaat gtgagtgcag
     2581 atactgctgc attacaactt gtatatggca agccaatcat atcaaaatat ccttgtagct
     2641 ttccatcttc tcctggtgtt ccatgcatgc acattaatac tgcatcaaat gttatagttg
     2701 tattatttac atcaatcgta aagttatttt tatttacaaa aattttttca tttgcatcat
     2761 tcaaatacca ccatccttct gggtttatgt ctattatata ccagtcaaac ttttgcttat
     2821 caatattatt agttactgta attacgcttt tataactaat ttctgcttct ttactgtacc
     2881 cgcctgtaac aattgcaata cattttttca tgggtttatt tttaatactt ctttttaatt
     2941 tttttaaatt atttatggta cagaaataat ttaaaaatat tctatttaat aagtaatttt
     3001 aatggtactt aattttttgt tgaaattaat agttacaaag taatattaaa aattagttgt
     3061 tttttgggta acaattaata attaatgaaa atttagtaaa acaatattaa gaaggcctct
     3121 ataaaatgtt caaattttgt taagtataaa aaaactaggg ctacttttaa gtttaaaacg
     3181 tattaatata ataaattgta cttttaatat tatgcagtgg cataaaaaaa atactatctt
     3241 tctccttatt ttttctttgt ttttattttt ttctaaagaa ataaaggcac aaggtaaata
     3301 tgatacaata aaagtatatg catatatcac agcctatggc gatacaattc ctatgagttt
     3361 tttaccacct gtagaggtga gtacaaaact ttcgagagca gctagaaggc atttaactga
     3421 atggaatagg cttagaaaag cagtttatgt tacttaccct tatgctattg cagcaagtag
     3481 aataatgaat gaagttaatg ctaagctggt tggtgtgaaa aataaaaagc aaagaaaaaa
     3541 aattatcaga gccagagaaa aagaattgaa gaaagaattt gctgataaac taactcaaat
     3601 gtcggtttat caaggaaaag ttttaatgaa attaattaat cggcaaaccg gaaataattg
     3661 ttatgaaatt atagatgaat acaaaggcag ttttactgct gcattttatc aaacaatagc
     3721 tattattttt ggaagtaatt taaagcaacc ctatgatgct aagggtaaag atgctgatat
     3781 ggaaaaaatt gtaattgatg tagaaaaaat gtatggatac agaagctaag taaatttttt
     3841 ttaatgacac cagaaagaac agaaaaatta ctaaatgtat taagtaggcg gcagcataat
     3901 ttaacagtag taatggagaa tgtacaagac ccacataaca tctctgcagt aatgcgtact
     3961 tgcgatgctg ttggaataca agatatttat attctaaata caaaaattcc aagacataaa
     4021 aagtttggag caaaaagtag cagtagtgca gcaaaatggc ttactgtaca tcaatttgat
     4081 aatgtgcaag attgttttaa cgaattaaga acaaagtata ataaaatatt tactactcat
     4141 ttatctacag gtgcagtaag tttgtatgaa ataaatttta ctgaaaatat agcattagtt
     4201 tttggtaatg aacatagtgg tgtatcagaa gaagt
//
LOCUS       DAOQHP010000185         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12471_length_4235_cov_3.359569, whole genome shotgun sequence.
ACCESSION   DAOQHP010000185 DAOQHP010000000
VERSION     DAOQHP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12471_length_4235_cov_3.359569"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLY81_06710"
     CDS             <1..382
                     /locus_tag="PLY81_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29955.1"
                     /translation="FLPLIPAPKLNCNVKLQCKQVGKLFTNAYFKIGFDYFLEQNKYY
                     KANFSETLTPAYWLLNFGFGGNLKLFNKKDMLSLFFSADNFLDVSYQSHLNRLKYAPQ
                     NKLTGKNGVFNMGRNFCIKTIFNF"
     gene            458..1144
                     /locus_tag="PLY81_06715"
     CDS             458..1144
                     /locus_tag="PLY81_06715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNF29956.1"
                     /translation="MKARNLIILILVVLFADQALKFYIKLNYYTGEEHKVIGNWFRLH
                     FVENEGMAWGWKFGGGFGKIALTLFRLAAVIWGTFLLRDFIKKKYHKWFIVCAALIYI
                     GALGNLIDSMFYGLIFDISYDEYHTHFYGQVAKAFTGVHYGSFLHGKVVDMFYFPLLK
                     GNFPEWMPFWGGEEFEFFRPVFNIADASISVGIITILIFQNKFFNQKQVANTNTVETS
                     TEVNDTTQVL"
     gene            1174..1794
                     /locus_tag="PLY81_06720"
     CDS             1174..1794
                     /locus_tag="PLY81_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF29957.1"
                     /translation="MIQKLYFHNFIKASIISLVAAIILFYFSFTLGKHPFFLLLNNNL
                     GIVADYFFKFYTHFGDGTIWAILLFLFIKFNKKYIPFLIAAFILCTLLVQVCKYVILP
                     NEPRPIAAIQPTSLIHTVQGVEPHTISTFPSGHTAAAFCFFFIASLFIHKKWIIPVGF
                     LYAILVGYSRVYLAQHFPFDVAAGIVTAIIAISFSIFIQKIFEKKK"
     gene            complement(1813..3081)
                     /gene="ricT"
                     /locus_tag="PLY81_06725"
     CDS             complement(1813..3081)
                     /gene="ricT"
                     /locus_tag="PLY81_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNF29958.1"
                     /translation="MGCSSCSKSNGKPNGCQSNGGCSSGSCNRLNVHDWLVNLPIDDY
                     ESSCKIVEVSFKQGSRKEFYRNPTLQFFEKGEYVTVEGVNGFDVGEVSLSGELVRVQM
                     KKKGVEEFNPEIKKILRRSSDRDIEIFKINKDREPSVQAKSRELAIIHKLQMKISEVE
                     IQADGKKATFFYTADDRVDFRELIKQYASEFKLKVEMRQIGIRQEAAKVGGIGSCGRE
                     LCCSTWLHDFKSVNTTAARYQNLSINQTKLSGQCGRLKCCLNYELDTYLDALQHFPDN
                     ADTFETTKGRAFLIKKDIFKNLMWYVLEGGNKHYPLTIQRVKEIKKLNAQGNKPDDLE
                     AVEVTSSKPKEVEPQFVDVVGQISLRSLEKSDSRRRNQQRDQRVDKRGNERNLKINTD
                     RNRNHLNTNQPKQNQQPNSGNNQPPPRKRN"
     gene            3470..>4235
                     /locus_tag="PLY81_06730"
     CDS             3470..>4235
                     /locus_tag="PLY81_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF29959.1"
                     /translation="MIEQINEGYVKVESEHGIVTIEFHHPQSNSLPGKILDALAKEIH
                     AAGINPNTRVIILQSTGTKVFCSGASFDELASIKTEEEGLKFFSGFAKVINAMRKTPQ
                     LIIARVHGKCVGGGVGLAAAADYAIACDGAEVKLSELAVGIGPFVVGPAVERKLGLSA
                     FSQLAIDANLWRPADWARRKGLFAELHNEVTGMDDSIARLSLNLSKASPAAMHELKKI
                     FWQGTEHWDELLKERAAISGKLILSDFSKNFIAKFKE"
BASE COUNT         1326 a          668 c          651 g         1590 t
ORIGIN      
        1 attcctgcct cttatacctg caccaaaact taattgcaat gtaaaacttc agtgtaaaca
       61 agtaggtaaa ttgttcacca atgcctattt taaaattggg tttgattatt ttttagaaca
      121 gaataaatat tataaagcta atttttctga aaccctaaca ccagcttatt ggttattaaa
      181 ctttggcttt ggagggaatt tgaaattgtt taataaaaaa gatatgctaa gtttattttt
      241 tagtgccgat aattttttag atgtttctta tcagagtcat ttaaacagat taaaatatgc
      301 acctcaaaat aagctaactg gcaaaaacgg tgtttttaat atgggcagaa acttttgtat
      361 aaaaacaatt tttaattttt aatgttaata caatattcac ttttatcagt agccatttct
      421 cttatctttg ccccacttaa tttttaagca tactatagtg aaagcaagaa atttaattat
      481 tttaattttg gttgttttgt ttgccgacca agcattaaag ttttatataa aacttaatta
      541 ttatacagga gaggaacata aagtaattgg taattggttt aggctgcatt ttgttgaaaa
      601 tgaaggaatg gcttggggtt ggaaatttgg tggcggtttt ggtaaaattg ctttaacctt
      661 atttaggctg gctgcggtaa tttggggtac ttttttatta agagatttta tcaaaaaaaa
      721 ataccacaaa tggtttattg tttgtgctgc attaatttat attggtgcat tgggtaactt
      781 aatagatagc atgttttatg ggttaatatt tgatataagc tacgatgagt accatactca
      841 tttttatggc caggttgcaa aggcatttac tggtgtacac tatggaagtt ttttacatgg
      901 taaggtggta gatatgtttt actttccatt attaaaaggt aattttccgg aatggatgcc
      961 attttgggga ggagaggaat ttgagttttt tagaccagtt tttaatattg cagatgcttc
     1021 tatatctgtt ggtattatta ccattcttat ttttcagaat aaatttttta atcaaaagca
     1081 agttgcaaat accaacacag ttgaaacctc aaccgaagta aatgatacaa cacaagtgtt
     1141 gtaattattt attctttttg caataataat tttatgatac aaaaattata cttccacaat
     1201 tttattaaag caagtataat tagtttagta gctgctatta tactttttta tttttcgttt
     1261 acgttgggta aacatccctt ttttctatta ctaaataata atttaggtat agttgccgat
     1321 tattttttta aattttatac ccactttggt gatggtacta tttgggctat tcttttattc
     1381 ctttttataa aattcaataa aaaatacatt ccttttttaa ttgctgcatt tatactgtgt
     1441 accttattag ttcaggtttg taaatatgta attttaccta acgaaccaag gcccatagct
     1501 gctattcagc caacaagcct aatacatact gttcaaggag tagagcctca tactatttca
     1561 acatttccat ctgggcatac tgctgctgct ttttgcttct tttttatagc ttcattgttt
     1621 atccataaaa aatggataat tcctgtaggt tttttgtatg ctattttggt tgggtattca
     1681 agagtttatt tggcccaaca ctttccattt gatgtagctg ctggtattgt tacagccata
     1741 atcgctataa gtttttccat ctttattcaa aaaatatttg aaaaaaagaa gtaaccataa
     1801 caatctataa aattaatttc tctttcgtgg cggtggttga ttgttaccac tatttggctg
     1861 ctgattttgt tttggctgat ttgtatttag atggtttcta tttctgtcag tatttatttt
     1921 taaatttctt tcattacctc tcttatctac cctttgatct ctttgttgat ttcttcttct
     1981 gctatccgat ttttctaaac tacgcaaact aatttgccca actacatcta caaattgtgg
     2041 ttccacttct ttaggttttg aacttgttac ttcaactgcc tctaaatcat ctggtttatt
     2101 gccttgagcg tttaatttct taatttcttt aaccctttgt atggttaagg gataatgttt
     2161 gttaccacct tctaacacat accacataag gtttttaaaa atatcttttt taattaaaaa
     2221 tgccctgcct tttgtagttt caaaagtatc tgcattatcc ggaaaatgtt gtaaagcatc
     2281 taaataagta tctagctcgt aatttaaaca acattttagc ctgccacatt gcccactaag
     2341 ttttgtttga ttaatactta agttttgata acgtgccgct gtagtattta cacttttaaa
     2401 atcatgaagc caagtgctac agcacaactc tcttccacaa cttccaatac cgccaacttt
     2461 agctgcttct tgtctaatac caatttgcct catttcaact tttaacttaa actctgaggc
     2521 gtattgttta attaattctc taaaatcaac acggtcatcg gcagtataaa aaaaagttgc
     2581 ttttttgcca tcagcctgaa tttcaacctc actaattttc atttgtaact tatgtataat
     2641 ggccaattct ctgctttttg cctgaacgct aggctctcta tctttattta ttttaaaaat
     2701 ttcaatatct ctatcgctac ttcttcgtaa tatttttttt atttctgggt taaattcttc
     2761 tactcccttt tttttcattt gtactcttac caattcacca cttaacgaaa cttctccaac
     2821 atcaaagcca ttaactccct ctacggttac atattctcct ttttcaaaaa attgtaaagt
     2881 tgggtttctg taaaattctt ttctactacc ttgcttaaaa ctaacttcta ctattttaca
     2941 actactttca taatcgtcaa ttggtaaatt tactagccag tcgtgtacat tcaatctatt
     3001 acagctacca gagctgcaac ctccattgct ttgacatcca ttgggtttcc cattgctttt
     3061 gctacaagaa ctgcatccca ttatatatac agttaaaaaa tgaataaatg ttttggagaa
     3121 attttaagaa aactaagcag attggttaca ttaccctact gtaaggctat tattatatat
     3181 gaatttataa aaataaaata gtaagctttt agtttaatta gtttatataa taaataaggt
     3241 aatttgtagt tctacttaaa aatttatact tcaaaatctg tttctccttt aagaatttat
     3301 ttcttaaaat ttctctacct ttcaaaaata agagttttat tttaaatgaa aactatctta
     3361 cacctactgg gtactaatta acaaaactta aattaataaa aaactatctt agtaacctca
     3421 tttaccaaat aaattttaaa aaatatcgtt tcttataaaa aaataaagta tgattgagca
     3481 aattaatgaa ggatatgtaa aagttgaaag tgagcatggt attgtaacaa tagagtttca
     3541 tcacccacaa agcaactcat taccaggtaa aattttagat gctttggcta aagaaataca
     3601 tgcagcaggt ataaacccta acactagagt tattatttta caaagtaccg gaactaaggt
     3661 tttttgcagt ggtgcaagtt ttgatgaact agcctccatt aaaacagaag aagagggact
     3721 taaattcttt tctggctttg caaaagttat aaatgccatg cgtaaaacac cacagctaat
     3781 tattgcaaga gtgcatggta agtgtgtggg tggtggtgtt gggcttgcag cagcagcaga
     3841 ttatgccatt gcttgcgatg gtgcagaagt aaaactaagt gagcttgcag taggcattgg
     3901 accttttgtt gttggccctg cagttgaaag aaaattaggc ttaagtgcct tttcacaatt
     3961 agcaatagat gctaatttgt ggaggccagc agattgggca agaagaaaag gactatttgc
     4021 agaattgcat aatgaggtta caggaatgga tgatagtatt gctagattat ctttaaattt
     4081 atcaaaagcc agccctgccg caatgcatga attgaaaaaa atattttggc aaggaacaga
     4141 acattgggac gaattgctta aggaaagggc agcaattagc ggaaaattga ttttaagcga
     4201 ttttagcaaa aattttattg caaaatttaa agaaa
//
LOCUS       DAOQHP010000186         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12504_length_4226_cov_3.748502, whole genome shotgun sequence.
ACCESSION   DAOQHP010000186 DAOQHP010000000
VERSION     DAOQHP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_4226_cov_3.748502"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="coaBC"
                     /locus_tag="PLY81_06735"
     CDS             <1..923
                     /gene="coaBC"
                     /locus_tag="PLY81_06735"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNF29960.1"
                     /translation="TLAKMANGICDNVLMAIYLSATCPVVVAPAMDEDMWKHAATKRN
                     MATLKKDSVQFIDVNNGELASGLIGEGRMAEPEEIFTFLIENFFRTTEFVGKKILITA
                     GGTIEAIDPVRFISNHSTGKMGFAIAEAFYLQGAEVIIVAGNVQVTTKYKGIKVVSVT
                     SADEMYKATLQYFKLSNIAVLSAAVADYKPITTAKEKIKKKEQILSLNLIKNKDILAT
                     LGNTKKRNQFVVGFALETNNENENALNKLKSKKVDVIILNSLKQKGAGFKTNTNQISI
                     YNKNGSVINFDLKTKAAVANDIVKYIKNSI"
     gene            977..1846
                     /locus_tag="PLY81_06740"
     CDS             977..1846
                     /locus_tag="PLY81_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNF29961.1"
                     /translation="MNAQELQAKVTVIANRIPTTVDKKIFTTLQTQLNNFMNNRKWSN
                     DVYKANEKIVCNFLLNLESMEDVNIYKASLTIQSARPVYNSAYQAALINYMDADVTFK
                     YVEYQPIEFNENRVQGTDALAANLTAVFAYYAYMILGLDYDSFSPKGGQAFFMKAQNI
                     VNNAPEAKGISGWRVFDGLRNRYFLVENLINSRYNILHDVIYSYYRNGLDKMYEAEKE
                     SRSKILQTLHQLVSFNKENANTMFLQFFMQNKSAELIGIFKNGTPEEKHQAIEALSLI
                     DITNTAKYKEQLR"
     gene            1851..2291
                     /locus_tag="PLY81_06745"
     CDS             1851..2291
                     /locus_tag="PLY81_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNF29962.1"
                     /translation="MKQIFFSILLMLFVVGCNYNSRIEENEMKIIVWEISCVDELINH
                     THLKDQDTSKKYTAQRDSLYQKIFAMHNTTADKFYKTFSYYEENPIQFKLLLDSALAY
                     GIRQRSNVIQTVKETIPTQPIQTTGTTTKDSLRKLTLKRNKALE"
     gene            complement(2341..3417)
                     /gene="gcvT"
                     /locus_tag="PLY81_06750"
     CDS             complement(2341..3417)
                     /gene="gcvT"
                     /locus_tag="PLY81_06750"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNF29963.1"
                     /translation="MKTTPFTQKHIALGAKMVEFAGYNMPISYTSINEEHEAVRTAAG
                     VFDVSHMGEFILKGEHALDLIQRTTTNDASKLTAGKAQYSCLTNEQGGIVDDLIIYCI
                     EENKVYLLVVNASNIEKDWNWISKFNTNNVEMHNISAKTCLLAIQGPTAVQLLQPLVK
                     SDILNLKYYTFVKDNFAGVDNVLISATGYTGAGGVEIYFEDTNGAADKIWDTILSLGI
                     KPIGLATRDTLRLEMGYCLYGNDIDDTTSPLEAGLGWITKFSKEFTAKAIIEKQKADG
                     IKKKLVGFEMLERGIPRHNYIIKDASGNTIGNVTSGTQAPSLGKAIGLGYVNIANAAI
                     DSEIFIDIRNNPVKAKVVKLPFSK"
     gene            complement(3533..4078)
                     /locus_tag="PLY81_06755"
     CDS             complement(3533..4078)
                     /locus_tag="PLY81_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF29964.1"
                     /translation="MKNIFYILFICVLMISCKASFLKELSTVNYETSRDEGHLDNHKI
                     LKGTINRNILLQDTAFKWFTDNYQYANPDATAVDAFNKNKDKFKVLVFAGMWCEDSRN
                     LLPLFYKLTDNSNFSEDNIYLIGVDRAKTTINNLHTKFNITHIPTFIILHKNKEIGRV
                     VEYGRTGMIDKELGAIVKPIK"
BASE COUNT         1559 a          606 c          607 g         1454 t
ORIGIN      
        1 atactttagc taaaatggca aatggtattt gcgataatgt tttaatggca atttatttat
       61 ctgctacatg ccctgtagtt gttgcaccag caatggatga agatatgtgg aaacatgctg
      121 ctactaaacg aaatatggca acattaaaaa aagactctgt acaatttata gatgtaaata
      181 atggagaatt ggcaagtggc ttaataggag aaggaagaat ggctgagcct gaagaaattt
      241 ttactttttt aattgaaaat ttttttagaa caaccgagtt tgtaggaaaa aaaatattaa
      301 ttactgcagg aggtaccata gaagctattg acccagttag atttatatct aaccattcaa
      361 caggtaaaat gggctttgcc attgcagaag ctttttattt acaaggtgca gaagtaatta
      421 tagttgcagg taatgtgcaa gtaaccacaa aatataaagg tataaaagta gtttcagtta
      481 caagtgcaga tgaaatgtat aaggctacct tgcaatattt taaactttca aatattgctg
      541 ttttaagtgc tgctgttgca gattataaac ccataacaac agcaaaagaa aaaataaaaa
      601 agaaagaaca aatcctatct cttaacctca taaaaaacaa agatatactt gctactttag
      661 gaaacaccaa aaagagaaac cagtttgttg ttggctttgc tttagaaacc aataacgaaa
      721 atgaaaatgc attaaataag ttaaaatcaa aaaaagtaga tgttattata ttaaattcgt
      781 taaaacaaaa aggtgcaggt tttaaaacaa acacaaacca aattagcatt tataacaaaa
      841 atgggtctgt aattaatttt gatttaaaga caaaagcagc tgtagctaat gacattgtaa
      901 agtatataaa aaattcaatt taatgaatta caaattaatc ataattttcc tactatcttt
      961 ttcttgtttt tcccaattga atgcacagga gttacaagca aaagttactg taatagcaaa
     1021 tagaatacca actactgtag ataaaaaaat atttactacg ttgcaaacac agctcaataa
     1081 ctttatgaac aaccgtaaat ggagtaatga tgtttataaa gcaaatgaaa aaattgtttg
     1141 taattttttg ttgaatttag agagcatgga agatgtaaat atctacaaag catctctaac
     1201 aattcaatca gccagaccag tgtataactc tgcctatcaa gctgctttaa taaattatat
     1261 ggatgctgat gtaactttta aatatgtaga ataccagcca atagagttta atgaaaatag
     1321 ggtacaaggt accgatgcat tggcagctaa tttaactgca gtttttgctt attatgctta
     1381 tatgatatta ggcttagatt acgattcctt ttctccaaaa ggtgggcaag cattttttat
     1441 gaaagctcaa aatattgtaa acaatgctcc agaggctaaa ggaatatctg gttggagagt
     1501 ttttgatggc ttaagaaaca ggtatttttt agttgaaaat ttaattaata gcaggtataa
     1561 tattctgcat gatgttattt acagttatta tagaaatgga ttagataaaa tgtatgaagc
     1621 tgaaaaagaa tctagaagta aaatattgca aaccttacat caactagtta gttttaataa
     1681 agaaaatgct aatactatgt ttttgcaatt ttttatgcag aataaatcgg cagaattaat
     1741 aggtattttt aaaaatggca ctcccgaaga aaaacaccaa gcaattgaag cacttagttt
     1801 aattgatata accaacacag caaaatataa agaacaacta aggtaacaaa atgaagcaaa
     1861 tatttttttc tatcttatta atgctgtttg ttgttggttg taattacaat agcagaatag
     1921 aagaaaatga aatgaaaata attgtttggg aaatatcttg tgttgatgag ttaattaatc
     1981 atacacattt aaaagatcaa gacacttcca aaaaatatac tgcacagaga gattctttat
     2041 atcaaaaaat atttgcaatg cataatacaa ctgcagataa attttataaa acttttagtt
     2101 attatgaaga gaatccaatc caatttaaac tacttttaga ttctgcattg gcttatggaa
     2161 taaggcagag aagtaatgta atacagacag ttaaagaaac catacctaca caacctatac
     2221 aaactacagg tactacaact aaagatagtt taagaaaatt aacattaaaa agaaataagg
     2281 cgttagaata attatcaaac gccttactgc atatattata tattatatct tagctcttaa
     2341 ttattttgaa aagggtagtt ttacaacttt tgcttttaca ggattatttc taatatctat
     2401 aaatatttca gaatcaattg cagcattagc aatgtttaca taacctaagc caatagcttt
     2461 gcctaagctt ggtgcctgtg taccacttgt aacattacct attgtatttc cagatgcatc
     2521 tttaataata taattgtgtc gaggaatacc tctctctaac atttcaaagc caactaattt
     2581 cttttttata ccatcggcct tttgtttttc tataattgct tttgcagtaa actctttgct
     2641 aaattttgtt atccagccaa ggccagcttc taatggagaa gtggtatcgt ctatatcgtt
     2701 tccatataag cagtagccca tttctaacct taaagtatct cttgtagcta atccaattgg
     2761 ttttatacca aggcttaaaa tggtatccca aattttatct gctgccccat ttgtatcttc
     2821 aaaataaatt tctactccac cagcacctgt ataacctgta gcactaatta aaacattatc
     2881 tacaccagca aaattatctt ttacaaatgt gtaatacttt aaatttaaaa tatcagattt
     2941 aactaatggt tgtaataatt gtacagcagt tgggccttgt attgccaata aacatgtttt
     3001 agcacttatg ttgtgcatct caacattatt agtattaaat ttacttatcc aattccaatc
     3061 tttttcaata ttactagcat ttacaaccaa taagtaaact ttattttctt caatgcagta
     3121 tataattaaa tcatcaacaa tacctccttg ttcgttagta aggcaactat actgtgcttt
     3181 tcctgctgta agtttagaag catcattagt tgttgtacgt tgaattaaat ctaaagcatg
     3241 ttcgcctttt aaaataaatt cacccatatg gcttacatca aaaactcctg ctgcagttcg
     3301 tacagcttca tgttcttcgt taatacttgt ataagaaata ggcatattat agcctgcaaa
     3361 ttcaaccatt tttgcaccta atgctatatg tttttgtgta aatggagttg tcttcattgt
     3421 aaaaatattt aaagtattca aaaaaatttt ggcaaatgta ctataacaaa tactgaaaat
     3481 tagaaattat aatttcatta atagagttgg aaaataaatt caagaatatt aattacttta
     3541 ttggtttaac aattgcacct aattctttat ctatcattcc tgttctgcca tattcaacca
     3601 ctctgcctat ttctttgttt ttgtgcagaa taataaaagt tggaatatga gtaatattga
     3661 atttagtatg taaattattt attgtggttt tggctctatc cacaccaatt aaataaatat
     3721 tatcttcaga aaaattacta ttatctgtta atttataaaa taaaggcaat aagtttctac
     3781 tgtcttcgca ccacattcct gcaaaaacta atactttaaa tttgtctttg ttcttattaa
     3841 aagcatcaac tgctgtggca tcaggattgg catattggta attatctgta aaccacttaa
     3901 aagcagtgtc ttgtaataaa atatttctat taattgtgcc ctttaatatt ttatggttat
     3961 ctaaatggcc ttcatctcta ctagtttcat aatttacagt tgaaagttct tttaaaaaag
     4021 aagctttaca ggaaatcatt agaacacaaa taaataagat gtaaaatata ttcttcataa
     4081 tttttgttta tgcttaaaat gttttaagta tctgaagtta ttttaaatta atttgaatta
     4141 atgaaagcat tcattaatta tttttaacta ataaactcgt tttaatttcg gttaaaaatg
     4201 atttaagttg aataagagat tgaata
//
LOCUS       DAOQHP010000187         4223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12519_length_4223_cov_3.954415, whole genome shotgun sequence.
ACCESSION   DAOQHP010000187 DAOQHP010000000
VERSION     DAOQHP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12519_length_4223_cov_3.954415"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY81_06760"
     CDS             complement(<1..391)
                     /locus_tag="PLY81_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNF29965.1"
                     /translation="MKSSLLLLSFLVFSFTSFAQDKKEVSEEIKVWGNCGMCKSRIEK
                     TLKGLGVTSASWSSETKMLSVKYNSEKLNNAKIQKAIAAVGHDTQDETASDEVYSKLP
                     GCCKYERKAVATEQKSNVSATTTTAKSC"
     gene            complement(488..880)
                     /locus_tag="PLY81_06765"
     CDS             complement(488..880)
                     /locus_tag="PLY81_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29966.1"
                     /translation="MKKLIVSIIAVLYFTVTSGMVVNFHFCKGKLSSIDFNVSPNKSC
                     ACKVKSKFNKNKSCCKSEYKVVKLNNLHNATYSNIDCKKPFYLAQPAVTFYQIISPKI
                     ATGFLVDNIVKPPISYQKAYIINCVFRI"
     gene            985..1632
                     /gene="msrA"
                     /locus_tag="PLY81_06770"
     CDS             985..1632
                     /gene="msrA"
                     /locus_tag="PLY81_06770"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNF29967.1"
                     /translation="MIKSIIFSLFSLSTIASIYFNFKSNNTLKMNDFQIIKEEAYFGA
                     GCFWCTEAIFQQVNGVIKVESGYSGGNLVNPTYEDICNKNTNHAEVVKITFNSKIITY
                     DELLEIFWQTHDPTTLNKQGADIGTQYRSIIFFTNTIQKQKAEDYKNALNKSGAFEKP
                     IVTIIEPIKKFYPAEAYHQNYYINNTSKGYCQFVIKPKIEKFKKVFKQKLKDKIE"
     gene            complement(1705..2079)
                     /gene="rplL"
                     /locus_tag="PLY81_06775"
     CDS             complement(1705..2079)
                     /gene="rplL"
                     /locus_tag="PLY81_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNF29968.1"
                     /translation="MADVKNLAEQLVGLTVKEVQELADVLKSEYGIEPAAAAVVVSAD
                     GGGGAAAVEEKTAFDVILVNGGASKLNVVKIVKELTGLGLKEAKDLVDGAPKPIKEGV
                     SKAEADDLVAKLKEAGAEVEVK"
     gene            complement(2179..2694)
                     /gene="rplJ"
                     /locus_tag="PLY81_06780"
     CDS             complement(2179..2694)
                     /gene="rplJ"
                     /locus_tag="PLY81_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNF29969.1"
                     /translation="MTKEQKNQVIEVLKEKFNQYNNFYITDTESLSVEQVGKLRKACF
                     DKQVEMKVAKNTLIKKALESLDGEKYAGVYESLHNVTALMFSENPKEPAVIISSFRKG
                     SNGEKPTLKAAFINGDVYSGDNQLKALTQIKTKNELIGEVIGLLQSPAKRVLAALLHH
                     HEKQAGAAVAE"
     gene            complement(2754..3446)
                     /gene="rplA"
                     /locus_tag="PLY81_06785"
     CDS             complement(2754..3446)
                     /gene="rplA"
                     /locus_tag="PLY81_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNF29970.1"
                     /translation="MSLTKKRKVANTKVDKNKVYSLKEASTLVKEINCTKFDSSVDLH
                     IRLGVDPKKADQQVRGTVSLPHGTGKTKRVLVLCTPDKEADAKNAGADFVGLDEFIAK
                     IEGGWTDIDVIVATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVAAAVNEVKGGKI
                     AFKVDKTGIVHASIGRVSFAPDKIAENSTELINAILKLKPATAKGTYLKGVSMASTMS
                     PGITLDTKSLSN"
     gene            complement(3473..3916)
                     /gene="rplK"
                     /locus_tag="PLY81_06790"
     CDS             complement(3473..3916)
                     /gene="rplK"
                     /locus_tag="PLY81_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNF29971.1"
                     /translation="MAKEISGFVKLQVKGGQANPAPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKMGKVVPVLITVYADKSFDFVIKTAPASIQLMEVAKVQKGSKESNRVKVGKVNW
                     QQIEAIALDKMPDLNCFTKESAMKMVAGTARSLGLTVEGNAPWEN"
     gene            complement(4101..4184)
                     /locus_tag="PLY81_06795"
     tRNA            complement(4101..4184)
                     /locus_tag="PLY81_06795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4148..4150),aa:Leu,seq:gag)
BASE COUNT         1403 a          712 c          568 g         1540 t
ORIGIN      
        1 aacaagattt tgcagtagta gttgtggcac ttacattact tttttgttca gttgctacag
       61 cttttctttc atatttacaa caaccaggta atttactata aacttcatca gatgctgttt
      121 catcttgcgt atcatgacca actgctgcta ttgccttctg aatttttgca ttatttaatt
      181 tttcagaatt gtatttaaca gatagcattt tggtttcaga actccaggaa gctgaagtta
      241 caccaagacc ttttaatgtt ttttctattc tgcttttaca cataccacaa ttaccccaaa
      301 ctttaatttc ttcagaaact tcttttttgt cttgagcaaa tgatgtaaac gaaaaaacta
      361 aaaatgataa taataataag gaagatttca tattttattt atttaaaatt attaataaag
      421 atagcaattt tattgccaat atttatagga tattacaaat taaaaactaa gggctaattt
      481 tcagattcta aattctgaaa acacaattta taatatacgc tttttgataa gaaattggag
      541 gtttaacaat attatcaact aaaaaccctg ttgctatttt tggtgaaatt atttgataga
      601 aggtaacagc aggctgtgct aagtaaaatg gcttcttaca atcaatatta ctgtaggtag
      661 cattgtgtaa attatttaat ttaacaacct tatattccga tttacaacaa cttttatttt
      721 tattgaattt tgattttact ttacaggcac aagacttatt tggtgaaaca ttaaaatcta
      781 tggaacttag ttttccttta caaaaatgaa aattaacaac cattccactt gtaacagtaa
      841 aatataaaac tgcaataatg gatactatta actttttcat tagttgcaaa actattaatt
      901 ttaaatgaaa taaatagcta aagtttaata aatatacaaa agcaaaaaat aatatttttt
      961 taaaccttat aaattgatta tcagatgatt aaatcaataa tattcagttt attctcttta
     1021 tcaacaatag cttctattta ttttaatttt aaaagcaata acacattaaa aatgaatgat
     1081 tttcagataa ttaaagaaga ggcttatttt ggtgcaggct gtttttggtg taccgaagct
     1141 atttttcaac aagttaatgg tgtaattaaa gtagaaagtg gatatagtgg tggaaattta
     1201 gtaaacccaa cttatgaaga tatttgtaat aaaaatacca accacgcaga ggttgtaaaa
     1261 attacattta attcaaaaat aattacttac gacgagttac ttgaaatttt ttggcaaaca
     1321 catgacccaa caactctaaa taaacaaggt gcagatattg gcacacaata tagaagtata
     1381 atttttttta ccaacaccat tcaaaagcaa aaagcagaag attataaaaa tgctttaaat
     1441 aagagtgggg cttttgaaaa accaattgtt acaattatag aaccaataaa aaaattttat
     1501 cctgccgaag cttatcatca aaattattat attaataata ctagtaaagg atattgccaa
     1561 tttgttataa aacccaaaat agaaaagttt aaaaaggttt ttaaacaaaa actaaaagat
     1621 aaaatagaat aaataaagtg tattaaagaa aatagtcgta accattacag ttacgactat
     1681 ttaattctat tagaaaagaa gtaattattt tacttcaact tcagcaccag cttcttttaa
     1741 tttagctact aagtcatcag cttcagcttt gcttacacct tctttaattg gttttggagc
     1801 accatccact aaatctttag cttctttcaa acctaaacct gttaactctt ttacaatttt
     1861 aacaacgttt aatttgcttg caccaccatt aactaaaatt acatcaaatg ctgttttttc
     1921 ttcaacagca gcagcaccgc caccaccatc tgcagaaaca acaactgctg cagcagctgg
     1981 ttcaatacca tactcagatt ttaacacatc tgctaattct tgtacttctt ttactgttaa
     2041 gcctactaat tgttcagcta agttttttac gtctgccata tttttaagtt ttaaccgctt
     2101 tcatcgtttt atctccagcg gaatggttaa taaatttgcc tcatttaaac ctcagagaca
     2161 ttggttattt aatttttatt attctgcaac agcagcacca gcttgttttt cgtggtggtg
     2221 caataaagca gccaacacac gttttgcagg agattgtaat aaaccaatta cttcgccaat
     2281 aagctcattt ttagttttaa tttgagttag agcttttaat tgattatcgc cactgtaaac
     2341 atcaccatta ataaatgcag cttttaatgt tggtttttcg ccattgcttc ctttacggaa
     2401 agatgaaatg ataacagcag gttcttttgg gttttcacta aacattaaag cagtaacatt
     2461 atgtaggctt tcataaacac ctgcatattt ttcgccatct aaactttcta atgctttttt
     2521 aattaaagta ttttttgcta ctttcatttc aacttgttta tcaaaacaag ctttacgtaa
     2581 tttaccaact tgttctacac ttaacgattc agtatctgta atgtagaagt tattatattg
     2641 attgaatttt tcttttaaaa cttcaatcac ttgatttttt tgttctttag tcataactaa
     2701 gttttattat aaacccattt cattttatac ttttaaagta taatccacag gctttagtta
     2761 gataatgatt ttgtgtctaa tgtaatacca gggctcattg tgctagccat gcttactcct
     2821 tttaagtaag tacctttagc agtagctggt tttaatttta atatagcatt aataagttcg
     2881 gtactatttt cagctatttt atcaggagcg aaagaaacac gaccgataga agcgtgaaca
     2941 atacctgttt tatctacttt aaatgcaatt ttaccacctt taacttcatt tacagcagca
     3001 gcaacatcgt ttgttacagt gccggtttta ggatttggca ttaagttacg aggacctaat
     3061 actttaccta atttaccaat tttaggcatt acagaagggg tagcaacaat tacatcaata
     3121 tctgtccaac caccttcaat ttttgcaata aactcatcta atcctacaaa atcagcaccc
     3181 gcattttttg catctgcttc tttatctgga gtacataaaa ccaacacacg ttttgtttta
     3241 ccagtaccat gtggtaagga aactgtacca cgtacttgtt gatctgcttt tttaggatct
     3301 actcctaaac gaatatgtaa atcaactgaa ctatcaaatt ttgtgcaatt aatttctttt
     3361 actagtgttg aagcctcttt aagggaataa actttgtttt tatccacctt tgtattggct
     3421 acttttcttt ttttagtcaa tgacatttta atttatttta atggtgaata atttaatttt
     3481 cccaaggtgc gttaccttct actgttaaac ctaagctacg tgctgtacct gcaaccattt
     3541 tcatagcact ttctttagta aagcagttca aatctggcat tttgtctaaa gcaatggctt
     3601 caatttgttg ccaatttact ttacccactt ttacacggtt actttcttta cttccttttt
     3661 gaacttttgc tacttccatt aactgaattg atgctggtgc agttttaata acaaagtcga
     3721 aagacttatc tgcatatact gtaattaaaa caggaacaac ttttcccatt ttatcttgag
     3781 ttcttgcatt aaattgtttg cagaactcca taatgtttac acccttagaa ccaagggctg
     3841 gacctactgg aggtgcagga ttggcttgac caccttttac ctgcaacttc acaaatccgg
     3901 agatttcttt tgccattttt ttagatttaa ttggtaataa cgttcatcat ttgatgaact
     3961 cccatatcgc aattattcat tgctttcaat cattcttatt taaaaagatc tttttgggag
     4021 tgcaaaagta agtgtattta ttgaaataat agaaataatt ttaaaaatac acttaagata
     4081 gctgatttta agattattag tgcccaggac aggatttgaa cctgcacgtt ctttcgaaca
     4141 ccaccacctc aaagtggctt gtctaccaat ttcaacacct gggcaatgta aaggtgcaaa
     4201 tgtattaagt gaaaataaat tat
//
LOCUS       DAOQHP010000188         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12673_length_4196_cov_2.920551, whole genome shotgun sequence.
ACCESSION   DAOQHP010000188 DAOQHP010000000
VERSION     DAOQHP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12673_length_4196_cov_2.920551"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLY81_06800"
     CDS             <1..468
                     /locus_tag="PLY81_06800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNF29972.1"
                     /translation="AYSKAIIEYINCDSLNKTFYLLDTFEGLVKEQITTEEEKVGVSN
                     YLKAYTNVYEQVKETFKNNNVKIIKGIVPDTLPMVTTNKIAYLSIDMNVTKPEIEALE
                     YFWDKMVSGGVLIHDDYGFPQHIHQKHAVDEFAKRKNVKVLCLPTGQAIIFKP"
     gene            471..1370
                     /locus_tag="PLY81_06805"
     CDS             471..1370
                     /locus_tag="PLY81_06805"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF29973.1"
                     /translation="MSILIIGSEGFIGSNLVNYFKAQHDNVICADIKAIQRNDYYQIN
                     PTDPNYDELFTNHSFSICINASGSANVKFSFDQPKLDFDSNTLSVFKLLNAIKNHQVN
                     CKFLNLSSAAVYGNFSTEPLSENIHCNPVSPYGFHKWYAELICKEYHVLFNLKTISVR
                     LFSVYGEGQEKLLFWDMWQKYKANNNNVELFGSGNEARDFLHIEDVVKAIDIVYKKED
                     FNGGAINIASGKTTTIKEAATTFFELLPAKNAQYSFTHHAKKGDPDILAADISKLQQL
                     GFTPTFNLHEGLSKYMQWLQEKK"
     gene            1349..2494
                     /locus_tag="PLY81_06810"
     CDS             1349..2494
                     /locus_tag="PLY81_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29974.1"
                     /translation="MVTRKKIVFPYYNSYYTSTAIIIYQTNILKSLNLLDDAIKPEIY
                     IWHTHQSPLHEIKTIQYPYIHYINIKSLGFRIKKALSKMVDVLGLSKTILFNTKYDCI
                     YPSYQDNFLNGIKERIYWKADFQENYFPQYITTVEMNWVKAFFKHLQNNDGNSTLVLS
                     SYDCKNDLEKFYPEIQNPVKLFRFVSHIPKQLNFENITTIQEKFNITLPYFIVCNQYW
                     PHKNHLVVLKALHQLKQENKLNFQVVFTGKTSSIRGNEYFTELKKFITENNLENDIVI
                     TDFVERTEQLILMKKSIAIVQPTTFEGWSTVIEDGKALNHYVIASDIKVNMEQIQENV
                     SFFNANDEATLAELLLKFSVEKPSINFIDYNNNIQNSINDLKNIFAI"
     gene            complement(2509..3939)
                     /locus_tag="PLY81_06815"
     CDS             complement(2509..3939)
                     /locus_tag="PLY81_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF29975.1"
                     /translation="MEIIHISAECYPVAKAGGLGDVVGALPKYQANAGHYSKVVMPMY
                     ETKFLHQNEFVEDFKGNIFLGNWQLPYTVIKEKNNTLGFDLYLVDIKGLLDRTGIYNY
                     DDDTERFMAFQLAVLDWMHHWQHNADIVHCHDHHTALIPFMMQHCYSIKNKLSSIPTV
                     LTIHNAQYQGWMSWNKNHYLPQYDTWKAGLLEWNNLINPLACGVKCAWQVTTVSHSYM
                     QELTWNSNGLEKLFEYEKGKCIGILNGIDTEVWNPKTDSFLTHHYNAKSVVVGKQRAK
                     EELCKTYQLDAQKPLFVFIGRLVGEKAADILPDAIMSILYSLPKSASFLILGSGEPEV
                     EWQLKELNKSFEGNYHAVIGYNESLSHQMYAGADFLLMPSRVEPCGLNQMYAMRYGTI
                     PIVRSTGGLQDTVIDIGEENGYGLRFNHATVGDMHNSILRAIKVFEDVKKILEMRRLC
                     MQINNSWENAVDNYLNLYEQLKIEKS"
BASE COUNT         1534 a          655 c          563 g         1444 t
ORIGIN      
        1 gcttacagta aagcaattat agaatatata aactgtgaca gtttaaataa aaccttttat
       61 ttattagata cttttgaagg attggtaaaa gaacaaataa caacagaaga agaaaaagtt
      121 ggagtaagca attacttaaa agcttatacc aatgtttatg aacaagtaaa agaaactttt
      181 aaaaataata acgtaaaaat tattaaaggt atagtgccag atacactgcc tatggttaca
      241 actaataaaa ttgcttattt atctattgat atgaatgtta ccaaaccaga aatagaggct
      301 ttggaatatt tttgggataa aatggtaagt ggaggtgttt taatacatga tgattatggc
      361 tttccgcaac atattcatca aaaacatgca gtagatgaat ttgctaaaag aaaaaatgta
      421 aaagtattgt gtttgcctac tggccaggca attattttta aaccttaatt atgtcaatac
      481 taattattgg ttctgaaggt tttataggaa gtaatttagt taattatttc aaagcacagc
      541 atgataatgt tatttgtgcc gatattaaag caattcaaag aaatgattat tatcaaataa
      601 atccaacaga ccctaattat gatgaattat ttaccaatca tagctttagt atttgcataa
      661 atgcaagtgg tagtgctaat gtaaaatttt cttttgacca gcctaaatta gattttgaca
      721 gcaacacttt atctgttttc aaattattaa atgcaataaa aaaccatcaa gtcaactgta
      781 aatttttaaa cctttccagt gctgcagtat atggtaattt ttcaacagaa cctttatcag
      841 aaaatattca ttgtaatcca gtatcgcctt atggatttca taaatggtat gcagagttaa
      901 tttgcaaaga ataccatgtt ttatttaatt taaaaactat aagtgtacgc ttattttctg
      961 tatatggaga aggacaagaa aaattattgt tttgggatat gtggcaaaaa tataaagcca
     1021 ataataataa tgtagaacta tttggcagtg gtaatgaagc aagagatttt ttgcatattg
     1081 aagatgttgt aaaagctata gatatagttt ataagaaaga agattttaat ggtggagcta
     1141 tcaatattgc aagtggcaaa acaacaacaa ttaaagaagc agcaactaca ttttttgaat
     1201 tattaccagc aaaaaatgca caatactcct ttacgcatca tgccaaaaaa ggcgacccag
     1261 atattttagc tgctgatata agcaaactac aacaattagg ctttacacct acatttaatt
     1321 tacacgaagg actttctaaa tacatgcaat ggttacaaga aaaaaaatag tttttcctta
     1381 ttacaatagt tattacacca gtactgctat tataatttat caaaccaata tattaaaatc
     1441 attaaacctt ttagatgatg ccataaagcc agaaatttat atttggcata cacatcaatc
     1501 tccattgcat gaaattaaaa ccatacaata tccatacatt cattacatca atataaaatc
     1561 gttgggcttt agaattaaaa aagctttatc taaaatggta gatgtgcttg gattaagtaa
     1621 aactatttta ttcaacacaa aatacgattg catttatcca tcttatcagg ataatttttt
     1681 aaacggaata aaagaaagaa tttattggaa agccgatttt caggaaaact attttcctca
     1741 atacattacc acagtagaaa tgaattgggt aaaagcattt tttaaacatt tacaaaataa
     1801 cgatggtaac agtactttag ttttaagtag ttacgattgt aaaaatgatt tagaaaaatt
     1861 ttatcctgaa atacaaaatc ctgtaaaatt attcaggttt gtatcgcata ttcctaagca
     1921 gttaaacttt gaaaatataa ctaccataca agaaaaattc aacattacac tcccctattt
     1981 tattgtgtgc aatcaatact ggccacataa aaatcattta gttgttttaa aagcattgca
     2041 tcaattaaaa caagaaaaca aactaaactt tcaagtagtt tttacaggaa aaacaagcag
     2101 tataagaggc aatgaatatt ttactgaatt aaaaaaattt attactgaaa ataatttaga
     2161 aaatgatatt gttattacag attttgtaga aagaacagag caattaattt taatgaagaa
     2221 atctattgct atagtacaac ccaccacatt tgaaggctgg agtacagtaa tagaagatgg
     2281 taaagcttta aatcattatg taatagcatc tgatattaaa gtaaatatgg aacaaataca
     2341 ggaaaatgta agtttcttta atgccaatga tgaagctaca ttagcagaat tattacttaa
     2401 attttctgta gaaaagccaa gtataaattt tatagactat aataacaaca ttcaaaactc
     2461 tatcaacgat ttaaaaaata tttttgcaat ataaatttgt atattttttt aagatttttc
     2521 tattttcaat tgctcatata aattcaaata attatctact gcattctccc agctattatt
     2581 tatttgcata caaagtcttc tcatttctag tattttcttt acatcttcaa aaactttaat
     2641 tgctcttaaa atactattat gcatatcgcc tactgttgca tgattaaacc ttaacccata
     2701 gccattttct tcacctatat ctattacagt atcctgtaaa cctcctgtgc ttcttactat
     2761 tggtatagta ccataacgca tagcatacat ttggtttagc ccacaaggct ctaccctact
     2821 aggcattaat aaaaaatctg ctcctgcata catttggtgg cttaatgact cgttataacc
     2881 tattactgca tggtagttac cttcaaaaga tttatttaat tcttttaatt gccattccac
     2941 ttctggctca ccactaccta aaattaaaaa acttgcactt ttgggtaagc tatataagat
     3001 actcataata gcatcgggta aaatgtctgc tgctttttca ccaactagcc ttccaataaa
     3061 aacaaataat ggtttttgtg catctaattg atatgtttta caaagttctt ctttggctct
     3121 ttgtttacca actactacac ttttggcgtt gtaatgatgc gttaaaaagc tatctgtttt
     3181 ggggttccaa acttccgtat caataccatt taaaatacca atgcattttc ctttctcata
     3241 ttcaaataat ttttctagtc cattactatt ccaggttaat tcttgcatgt agctatggct
     3301 aacagtagtt acttgccatg cacattttac accacaggct agtgggttaa ttaaattatt
     3361 ccattctaat aatccagctt tccaagtatc atactgtggt aagtaatggt ttttattcca
     3421 actcatccat ccttgatatt gtgcattgtg aatggttaaa acagtaggta tagaacttaa
     3481 tttattttta atactataac aatgctgcat cataaaagga attaaagctg tatggtgatc
     3541 atggcaatga acaatatctg cattatgttg ccagtgatgc atccaatcta aaacggccaa
     3601 ctgaaaagcc atgaatcgtt ctgtatcatc gtcataatta taaatacctg ttctatccaa
     3661 taatcctttt atatctacca aatacaaatc aaatccaagt gtattatttt tttcttttat
     3721 aacggtataa ggcagttgcc aattacctaa aaaaatattt cctttaaaat cttccacaaa
     3781 ctcattctga tgtaaaaatt tagtttcgta cataggcatt acaactttag aataatgccc
     3841 agcattagct tggtattttg gcaaagcacc tacaacatcg cctaagccac cagctttagc
     3901 aactgggtaa cactctgcac taatatgaat tatttccata tcaaattatt ttatttacct
     3961 actttgaata tctataaatg taaagcaaaa tgtacaatta aaattttatt ttataatttt
     4021 tcttcggaac ataaaatagt ttattttaca tgctttaaaa taatttcaac caaatgagta
     4081 ctgcacatta ccaaaccaaa tggtctcaac taggcacttt aattactgta tttttctttt
     4141 ggggctttgt tgcagccagt aacgatattt taattcctgt gtttaaacat gctttt
//
LOCUS       DAOQHP010000189         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12687_length_4193_cov_4.428468, whole genome shotgun sequence.
ACCESSION   DAOQHP010000189 DAOQHP010000000
VERSION     DAOQHP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12687_length_4193_cov_4.428468"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1058
                     /locus_tag="PLY81_06820"
     CDS             360..1058
                     /locus_tag="PLY81_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF29976.1"
                     /translation="MLQLKEFLLLWWEIYFPVLTNPSLYKVSLGGTFYFSMFQYFIIY
                     KPFNTLSQFSSTENKQTLAHYCKLAKNIYPVGRLDYDSEGLLILTNDKKLNNLLLNPN
                     YEHRRTYIVQVEGSITPIAIQQLNNGVSINVNGKNYTTKKADAAIISAPINLPNRNPP
                     IRFRNNIPTSFIQLTLTEGKNRQVRKMTAKTGFPTLRLIRYSIENVSINNMQPGDIIE
                     LSQKTIYNKLSIPL"
     gene            complement(1094..1618)
                     /gene="msrB"
                     /locus_tag="PLY81_06825"
     CDS             complement(1094..1618)
                     /gene="msrB"
                     /locus_tag="PLY81_06825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNF29977.1"
                     /translation="MNIKFYFYTILLVLSFTNCTAQNNNNNKSNMQKENKANPYYSNK
                     DTNKLVLKEEEWRKILTSEQFYIARQKGTEQPFSSKFENFKEKGTYYCAACGNALFIS
                     DTKFESGCGWPSFYQPINKSSIIYTPDTTHGMQRTEVQCGRCKAHLGHVFNDGPPPSG
                     LRYCINGVILDFKK"
     gene            1708..2283
                     /locus_tag="PLY81_06830"
     CDS             1708..2283
                     /locus_tag="PLY81_06830"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNF29978.1"
                     /translation="MLNVILFGPPGSGKGTQSEKLIAKYGFKHLSTGDLLRSEITAKT
                     ALGLEAKGLMDKGQLVPDEVVIGMISHALEANPKATGFLFDGFPRTEAQSVALDNLLK
                     KYNTQIGVFLALEVSEEELVKRLLNRGLTSGRSDDTNEEVIRARIIEYERKTMVVADY
                     YEKFKKVAHIKGEGSVEDIFNTLCNEINKRV"
     gene            complement(2363..3586)
                     /locus_tag="PLY81_06835"
     CDS             complement(2363..3586)
                     /locus_tag="PLY81_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF29979.1"
                     /translation="MNFLFAWRYFKSKKSTNAVNIIAYISIVAIMIGTAALILVLSVF
                     NGFESLVKNLYNDFYADAKIVPVKGKSFHLNNQQYQLLQTNSNIAYWSKVVEEKALLK
                     NSENQATVIVKGVQNNYTNIAPINKHIIRGSFDIGNTENPKIVIGSGIESATAIYLEQ
                     PLEDPVLFLPNKKAVNLNSINNAFLSYNVIPSGTFVLQQDFDNKYIFTNLPFMQFMLD
                     MDSTQFSYLELKLTTKSSTDEEKIIKQLQQQLGTQVKVLSRYEQNKSLYTIMQIEKWI
                     IYGILCLILIVAAFNMIGSLTMLILEKQKDIAILKAIGANDVTIKNIFITNGFILAFV
                     GSFIGILLATLICWLQIKFKLLKLSGSFVVDYYPVEMRWQDYLLVLFTVFIIAFIASF
                     IPASKANRQNINLKS"
     gene            complement(3682..4059)
                     /gene="rbfA"
                     /locus_tag="PLY81_06840"
     CDS             complement(3682..4059)
                     /gene="rbfA"
                     /locus_tag="PLY81_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNF29980.1"
                     /translation="MEEGKRQKQVAAVLQDELNNIFRKLGLNMMGGGMVSIAQVKVTP
                     DLLEARIYISFFKVEDIKAAMKKIDDKAWEIKRDLANHIKNQVRRIPVLHFYLDDTLD
                     YVFKMEDVFKKIETDKLNNNLAN"
BASE COUNT         1470 a          606 c          580 g         1537 t
ORIGIN      
        1 ttaaaaatcc tgatgataac tacatcagga ttttttgttg cttgaatttt ttcttctctg
       61 ttttttaatt tattcaatag gtacatgaac ctttttttta ttttttttta gaagttgaga
      121 aaaatatttt tagaatttca gtgaaaagtt tttttattct ttttgattca tgtttataaa
      181 ataaaatctc ataatccttt gtagcccttt atttgtaagt gtttcaaagt gttgtagtat
      241 taacagtaga aatatatttt cataaaatga gtactgaagt atgaaataat taataaagta
      301 attcttctgt caattcacaa aaaaaaataa aatgttgata agtttgtata tatttttttt
      361 tgttacagtt aaaagaattt ttattattgt ggtgggaaat ttatttccca gtacttacta
      421 acccatcatt atataaagtg tcgcttggcg gcacttttta ttttagcatg tttcaatact
      481 ttattatata taaacctttt aatacactca gccagttttc ctcaacagaa aacaaacaaa
      541 cacttgcaca ttattgtaag cttgcaaaaa atatttatcc tgttggtagg ctagattatg
      601 atagtgaagg tttattaatt cttaccaacg ataaaaaatt aaataactta ttacttaatc
      661 caaattacga acatagaaga acttatattg tacaagtaga aggtagcatt acacctattg
      721 caatacaaca attaaacaat ggtgtaagta ttaatgttaa tggtaaaaat tatacaacta
      781 aaaaagcaga tgctgcaata attagtgcac caattaattt accaaataga aatcctccca
      841 taagatttag aaataatata ccaactagtt ttatacaatt aacgcttacc gaaggtaaaa
      901 ataggcaggt aagaaaaatg actgcaaaaa caggatttcc aactttgcgt ttaataaggt
      961 atagtataga gaatgtatct attaataata tgcagcctgg agatataatt gaattatccc
     1021 aaaaaacaat ttataataaa ctttcaattc ctttataatt aagttactca atttgttact
     1081 caatttttat aaatcatttt ttaaaatcta aaatcactcc attaatgcaa taccttaacc
     1141 ccgatggtgg aggaccatca ttaaaaacat gacccaaatg agctttgcat ctgccacact
     1201 gtacttctgt tctttgcatt ccatgtgtgg tatctggcgt ataaataata gaagacttat
     1261 taattggttg ataaaaactt ggccaaccac aaccactctc aaatttggta tcacttataa
     1321 aaagtgcatt accacaagct gcacaataat aagttccttt ttctttaaaa ttttcaaact
     1381 tactactaaa aggttgctca gtgccttttt gccttgcaat ataaaactgt tcagaagtta
     1441 aaatttttct ccattcttct tcttttaata caagtttatt ggtatcctta ttactgtaat
     1501 atggatttgc tttattttct ttttgcatat tgcttttgtt attattattg ttttgagctg
     1561 tacaattagt aaaagacaaa acaagtaaaa tggtatagaa ataaaattta atattcatat
     1621 ttgaataata atttatctaa tttttttatt gtttacaaaa ttaacactca agccttatat
     1681 ttgttgttca agtaaggttt taggtacatg ttaaatgtta ttttatttgg tcccccaggt
     1741 agtggcaaag gcacacagag tgaaaaactt attgctaaat atggctttaa gcatttaagt
     1801 acaggcgatt tgctaagaag tgaaattact gcaaaaacag cattaggctt agaggctaaa
     1861 ggcttaatgg ataaaggtca gttggtgcca gacgaagttg taattggtat gataagccat
     1921 gcattagaag ccaatccaaa agctacaggc tttttatttg atggttttcc aagaacagaa
     1981 gcacaaagtg tagcattaga taatttatta aaaaaataca atacacaaat tggcgttttt
     2041 cttgccttag aagttagcga agaagagttg gttaaaagat tgttgaatag aggattaacc
     2101 agtggcagaa gtgatgatac caatgaagaa gtaataagag ccagaattat tgagtatgaa
     2161 agaaaaacaa tggtggtggc agattattat gaaaaattta aaaaagtagc acatataaaa
     2221 ggcgaaggca gtgttgaaga tatttttaat accctatgca atgaaattaa taaaagggta
     2281 taaattataa atccttaatt tttactacca ataccaactg caaccaatcc tactgagtat
     2341 tcttagtagg atttttttat tactaacttt ttaaatttat attttgcctg tttgccttac
     2401 ttgcaggaat aaatgaagct ataaatgcaa taataaaaac tgtaaatagc actaataaat
     2461 aatcttgcca acgcatttca acagggtaat aatctactac aaaacttccg ctaagtttta
     2521 gtagcttaaa ttttatttga agccaacaaa ttagggtagc taataaaata ccaatgaatg
     2581 aacccacaaa ggctaaaata aaaccattgg tgataaaaat attttttatg gttacatcat
     2641 ttgcaccaat tgcttttaaa atagcaatgt ctttttgttt ttctaatatt agcatggtta
     2701 atgaaccaat catgttaaaa gctgcaacaa ttaatattaa gcaaagaata ccataaataa
     2761 tccatttttc tatttgcatt atggtataaa gacttttgtt ttgctcgtat ctggatagca
     2821 ctttaacttg agtgcctaat tgttgttgaa gttgctttat aattttttct tcatcggtgc
     2881 tgctttttgt tgtaagcttt agttctagat acgaaaactg agtactatcc atatctagca
     2941 taaactgcat aaaaggtaaa ttggtaaaaa tatatttatt atcaaaatct tgctgtaaaa
     3001 caaaagtgcc gcttggtatt acattataag ataaaaaagc attgtttata ctatttaaat
     3061 ttactgcctt tttgtttggt aaaaataaaa cagggtcttc taatggttgc tctaaataaa
     3121 ttgcagtagc actttcaatg ccactaccaa ttactatttt agggttttct gtattgccaa
     3181 tatcaaaact accacgaatt atatgtttat ttattggtgc aatattggta taattatttt
     3241 gaacaccttt tacaattaca gttgcttggt tttcgctatt ttttaatagt gctttttctt
     3301 ctactacctt actccaatat gcaatatttg aattggtttg tagtaattga tactgctgat
     3361 tgtttaagtg aaagctttta ccttttacag gaacaatttt agcatctgca taaaaatcgt
     3421 tgtataagtt tttaactaaa ctttcaaatc cattaaaaac acttaacact aaaattaagg
     3481 ctgcagtgcc aatcataatt gctacaatac ttatgtatgc aataatgttt acggcattgg
     3541 tacttttttt acttttaaaa taacgccatg caaataaaaa gttcaacagc tataaaattt
     3601 acagattaat tatttttttg tactaagctt ttgtaaaact attattcatt tgttgcgtcg
     3661 cacacttcag ggctttgtac attagttggc taaattatta ttcaatttat cggtttctat
     3721 ttttttaaat acatcttcca ttttaaaaac ataatcaagt gtatcatcta aataaaaatg
     3781 taatacagga atacgcctaa cttgattttt tatatgattg gctaaatctc ttttaatttc
     3841 ccaagcttta tcgtcaattt ttttcattgc agccttaatg tcttcaacct taaaaaaact
     3901 aatataaatt cttgcctcta ataaatctgg agtaaccttc acttgggcaa tagataccat
     3961 accacccccc atcatattta agcctaattt tctaaaaata ttatttagtt catcttgcag
     4021 tacagcagct acctgctttt gtctttttcc ttcttccata catttaattt ttacaaaagt
     4081 acaaccttta atttagttgg tagatattga aaataagata gaggctaaga gacaaaattt
     4141 taagtaagaa atatttactc tttttatgat gtagcctgta ttaacattat ttg
//
LOCUS       DAOQHP010000190         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12728_length_4185_cov_3.912107, whole genome shotgun sequence.
ACCESSION   DAOQHP010000190 DAOQHP010000000
VERSION     DAOQHP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12728_length_4185_cov_3.912107"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLY81_06845"
     CDS             complement(<1..1314)
                     /locus_tag="PLY81_06845"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNF29981.1"
                     /translation="MRQFLIIAFLVLTTNIQAQFTVTPNNNAMQLAQKIVGQGVQIVN
                     AWFKGSSTSAGFFTDNTGTLGISSGVLLTSGKAANVAAAASSQNDATFGAGDVDLTNL
                     VGTGTGNDACVLEFEFIPVGDSIVVKYVFASEEYPEYACSDFNDVFGFLLKSPLPIFP
                     PNVFKNIALVPGTTIPVKINTINPGAGVNGQIGNCTSQGTGSPFTQYYRNNLSNSYII
                     YDGMTTILVAKAKVVPCQINRIKIGVQDMVDGVFDSGVFIEANSFRSDTTSLIKTGGY
                     TDAQGNKNVAVEGCSKVKVKITLEKPAGPSGKTLQLNYSGSATRGVDFDAALAPTSAV
                     FAAGQSDFELEIKPIVDAVVEPLDSVIISAGIACATSTAKITVYIKDSITFRNSKDTF
                     TCSSFPTVLTASYDSSNTNVYSWNVTPAVTTQTLSVTKPGVYIATN"
     gene            1494..2270
                     /locus_tag="PLY81_06850"
     CDS             1494..2270
                     /locus_tag="PLY81_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNF29982.1"
                     /translation="MQPLVDKINKQRLPKHIAIIMDGNGRWAEEKGEDRLFGHFHGVE
                     SVRNIVEGCAELGIQYLTLYAFSTENWDRPQQEVDGLMTLLVDTIRKEVPELNKNNIK
                     LHVIGNTSMLPELARNELNEAIDLLKNNTGLNLIMALSYSSRWELINAVQAIAKDVEM
                     GNLNPNSINNTVFEKYLTTANFPDPELMIRTSGEFRISNFLLYQLAYSELYFTNTCWP
                     DFRKNNLYEAIIEYQGRERRFGKTGKQIHDNIVSNSTSHP"
     gene            2443..>4185
                     /locus_tag="PLY81_06855"
     CDS             2443..>4185
                     /locus_tag="PLY81_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNF29983.1"
                     /translation="MSKRLSTKYLLLLFLTAFSTAQLSAQQNNKDTTITSVDAELINL
                     FNQKKPQKYKIAGITVVGNNYFDETLLVSISGLNIGDEIIIPGGDNFAKAIQKLWAQN
                     YFSDVAIYVSKLVDKSIWLEIHVTERPRLARFTFVGIKKSDKEDLIGKTGLVPNRIVT
                     ENMKKNAEDAIIKFYAEKSYRNVTVTIDEKKDSIINNAIELVFHINKGNKVKVNNIDF
                     ANVTLDPLKLKKQLKDTKEKSRFTIKKSDDKSAFSGITKYTFKDYLNENGFLTYSKTK
                     KLLDPYIRFKFLSSAKFNDKKFNGDIEKLLEYYNTNGYRDAVINKEDIKILPNAKGDL
                     NISIKVNEGKKYYFGNIAWRGNTKYSDSVLTSILGIKKGDVYNLETLKKGLGIQLSPE
                     GGDVSGLYMDDGYLFFRTEPIETAVYNDTIDYLIRVTEGPEATIKDVNITGNNRTKEH
                     VIRREIRTIPGEKFRRSDLIRSQREIAQLNFFNQEKIGINPRPNPEDGTVDIDYSLEE
                     KSSDQLELSAGWGGGIGLTGTVGVSFNNFSIKNIFKKQAWDPLPMGDGQKLSLRVQSN
                     GRAFRSYNFSFTEPW"
BASE COUNT         1579 a          625 c          689 g         1292 t
ORIGIN      
        1 attcgtagct atataaacac caggttttgt aacacttaaa gtttgagtag ttactgctgg
       61 ggttacattc caagagtaca cattagtatt ggaagagtcg tagctagctg taagtactgt
      121 agggaaagaa gaacaggtaa aagtatcttt tgaatttctg aatgtaatag aatctttaat
      181 ataaactgta atttttgcag tggaggtagc acaagcaatt cctgcagaaa taattacaga
      241 atctaatggc tcaacaactg catctacaat aggtttaatt tctaattcaa aatccgattg
      301 ccctgcagca aatactgcac ttgttggtgc aagggcagca tcaaaatcta caccacgtgt
      361 tgcagaacct gaataattta attgcaaagt tttaccgctt ggtcctgcag gtttttctaa
      421 agttattttt actttaactt tactacaacc ctcaacagct acatttttat ttccttgagc
      481 atctgtatat cccccagttt taattaatga tgtagtatca gatctaaaac tattcgcttc
      541 tataaaaaca ccagagtcaa aaacgccatc aaccatatct tgcacaccaa tcttaattct
      601 gtttatctgg cagggtacta cttttgcttt agcaaccaat attgtagtca tgccatcata
      661 aattatataa gagttggata aattatttct ataatactga gtaaaaggag agccagtacc
      721 ttgagaggta cagtttccta tttggccatt aacacctgct ccaggattaa ttgtgtttat
      781 tttcacgggt atggttgtac ccggcactaa cgcaatattt ttaaacacat taggaggaaa
      841 tataggtaat gggcttttta ataaaaaacc aaaaacatca ttaaagtcag agcaggcata
      901 ttcaggatat tcttctgatg caaatacata ttttaccaca atagaatcgc caactggtat
      961 aaattcaaat tctaaaacac aagcgtcatt accagtacca gttccaacta aattagttaa
     1021 gtcaacatct ccggcaccaa aagttgcatc gttttgtgag gatgctgctg cagcaacatt
     1081 ggctgcctta ccagaagtta ataatacacc actactaatt cctaaagttc cggtattatc
     1141 tgtaaaaaaa ccagcagagg tagaagagcc tttaaaccat gcatttacaa tttgcacccc
     1201 ttgtccaaca attttttgag ctagctgcat tgcattgttg ttgggggtta ctgtaaactg
     1261 agcttgaata ttagtggtta atactaaaaa ggcaattatt aaaaactgtc tcactgtagg
     1321 ggatataatt tgtttaaaag gttaaaacag gggctaaagt aaattattta aagatatttt
     1381 ttaaaaaata ttaataaact aaactataaa cgatactttt attatacttt ttgctgcttt
     1441 actttaatat aattatatgt attttactaa tttcgcagcc ttaaaagcaa tttatgcagc
     1501 ctttagttga taaaataaac aaacaacgcc ttccaaaaca tattgctatt attatggatg
     1561 gtaatggacg ttgggctgaa gaaaaaggag aagaccgttt gtttggacac tttcatggag
     1621 ttgaaagtgt acgaaatatt gtagagggtt gtgccgaatt gggtattcaa taccttactt
     1681 tatatgcttt tagtaccgaa aattgggata ggccacaaca agaagtagat ggattaatga
     1741 ctttattggt agatactatt agaaaagaag tgccagaact taataaaaac aatattaagc
     1801 tacatgtaat tggcaataca agtatgctgc cagaattggc aagaaatgaa ctgaatgaag
     1861 ctatagattt actaaaaaat aatacaggtt taaatttaat aatggcccta agttacagct
     1921 caagatggga gcttattaat gctgtacaag caatagcaaa agatgtagaa atgggcaatc
     1981 ttaaccctaa tagtattaac aatactgtat ttgaaaaata tttaacaact gctaattttc
     2041 cagacccaga attaatgata agaactagtg gcgaatttag aattagtaac tttttacttt
     2101 accagttggc ttactctgaa ctatatttta caaatacttg ttggcccgat tttagaaaaa
     2161 acaacctata tgaagccatc atagaatatc aaggcagaga gagaaggttt ggaaaaacag
     2221 gcaagcaaat acatgataat atagtttcaa attctactag ccatccttaa atcaattaat
     2281 agtactgtta ctatgtttaa aataaataat actgccctaa aaattaatat aactttttat
     2341 ttaacaaacc cttttaagaa atccctttaa tttgctttta aatcccttgc tgaaattgcc
     2401 tgtaattgca actttagaag acttataact taagacaaaa atatgtccaa acgcttatct
     2461 acaaaatatt tacttctgct ttttttaact gcttttagta cagctcaatt atcggcacaa
     2521 caaaataata aggatactac tattacatcg gtagatgcag aattaattaa ccttttcaat
     2581 caaaaaaaac cacagaaata taaaatagct ggtattacag tagtaggtaa taattacttt
     2641 gatgaaacat tattagtatc tatttcaggc ttaaatattg gcgatgaaat aataatacca
     2701 ggtggagata attttgcaaa agcaattcaa aaactttggg ctcaaaatta ttttagcgat
     2761 gtagcaattt atgtatctaa actagtagat aaaagcattt ggcttgaaat tcatgttacg
     2821 gaacgaccaa gacttgcacg ttttactttt gtaggaatta agaaaagcga taaagaagat
     2881 ttaattggca aaacaggctt agttcccaat cgtattgtta cagaaaatat gaagaaaaat
     2941 gcagaagatg caattattaa attttatgca gaaaaatctt atagaaatgt aactgtaact
     3001 attgatgaaa agaaagattc tataataaat aatgctatag aattagtatt tcatattaat
     3061 aagggcaaca aagtaaaagt aaataatatt gactttgcca atgttacttt agacccttta
     3121 aaactaaaaa aacaattaaa agatactaaa gaaaaaagtc gttttacaat aaaaaaatct
     3181 gatgataaaa gtgcattttc aggaataaca aaatatacat ttaaagacta tttaaatgaa
     3241 aatggttttt taacctatag taaaacaaaa aaattattag acccatatat tagatttaaa
     3301 tttttgtcat cggcaaaatt taatgataaa aaatttaatg gcgatataga aaaactgctt
     3361 gaatactaca atactaatgg ctatagagat gctgtaatca ataaagaaga tataaaaata
     3421 ttacccaatg caaaaggcga tttaaacatt agtataaaag taaacgaagg taaaaaatat
     3481 tattttggca atatagcttg gagaggaaac accaaatact ccgactctgt attaacttct
     3541 attttaggaa ttaaaaaagg agatgtttat aatttagaaa cattaaaaaa agggttaggc
     3601 attcaactat cgccagaagg gggtgatgta agtgggttat atatggatga tggttacctt
     3661 ttctttagaa ctgaacctat agaaactgct gtttataacg ataccataga ttatttaatt
     3721 agagttaccg aagggccaga agctaccatt aaagatgtaa atattacagg aaataacaga
     3781 accaaagaac acgtaataag aagagaaata agaaccattc ctggagaaaa atttagacga
     3841 agcgatttaa tacgttctca aagagaaata gctcaattaa atttctttaa tcaagaaaaa
     3901 ataggaatta atcctcgtcc taatccagaa gatggaacag tagatattga ttattctcta
     3961 gaagaaaaaa gcagcgacca attagaactt agtgcaggct ggggaggagg aattggatta
     4021 actggtacag taggtgtaag ttttaacaac ttctctatta aaaatatttt taaaaagcaa
     4081 gcttgggatc cattaccgat gggcgatggt caaaaactaa gccttcgtgt tcaaagcaat
     4141 ggtagagctt ttagatctta taatttttca tttaccgagc cttgg
//
LOCUS       DAOQHP010000191         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_12956_length_4136_cov_3.757412, whole genome shotgun sequence.
ACCESSION   DAOQHP010000191 DAOQHP010000000
VERSION     DAOQHP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_4136_cov_3.757412"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLY81_06860"
     CDS             <1..263
                     /locus_tag="PLY81_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF29984.1"
                     /translation="KEEVKGFYISPANAFLTNRVDAVIGSLWKVPDETTNLLLQEFYT
                     NIEKKGMTKVEALRHAQAAVSENPKYSHPFFWSAFVLYGEWR"
     gene            316..1653
                     /locus_tag="PLY81_06865"
     CDS             316..1653
                     /locus_tag="PLY81_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF29985.1"
                     /translation="MQSKQQLGFFSLTMIVISFVIGMGIFKTSSTVAAKAGTETIFYA
                     AWLIGGFIAICGALTYAEIGVRLPVTGGYYKIFAYAYHPSIGFTINILVLISNAASLA
                     VVALIGADYVSDLLFAKPSGTLFNIGIAALAIFLFYGVNLLGLQTSSKTQNVLTILKV
                     ALVVLLISSFFSNTQVEPHGYTNNTIYTYNGNNGALLLILSLVAVSFTYGGYQQTINF
                     GEEVKNTRTMQRSIITGMFIVVLLYFAINYVYVHVIGFDKMKNATAIGALLTKAWFGN
                     IGAKIFDACMFLSVLAYVNVVLLSNPRVMYAMSNDGVLPKIFSNKNNKTGVLIPGLTV
                     FALTALIITFFGKGVDDVLSFSIFLDCFGMSLSAATLLILRKRKQGEENVKGFLKNIT
                     PVLCILFVIAYSIVAVAVVIDKPMAALTGVILLAVFILVYFLFCHKKQTVKLP"
     gene            complement(1711..2874)
                     /locus_tag="PLY81_06870"
     CDS             complement(1711..2874)
                     /locus_tag="PLY81_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF29986.1"
                     /translation="MKRIFTLSLLFTFFSTSLLAQEVIARWNFEVLDFSSATGNTFTV
                     GAGSVVADSGALVTGSEFSANHAAATSTWVTPAGNGSANSASSSGWASGDYWQFKAST
                     TGFNSITIVYDQMGSNTGPKSFLVQYSTDGVTFTDVPSSGYDIANDAWSASTYKPVST
                     RNLSLAAITALNNQPAIYIRLTVAAGSTNINGAAIATGGTSRIDNFYIGGIALAPLTL
                     NSFTASAVNGKANLNWFTTNEVNVDGFDIEKSNNGKDFTSVGFVKANNKTENAYSFTN
                     ELNGISYYRLKMIDKDGTFKYSKTVSLNGKTTVKLDLYPNPVRNTTTLSHDKATDRAT
                     IKITTLGGKTVNVVYVQNGATQTTIDVSKLLNGNYIMVYENNGTRSSVQFVKQ"
     gene            3002..>4136
                     /locus_tag="PLY81_06875"
     CDS             3002..>4136
                     /locus_tag="PLY81_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29987.1"
                     /translation="MKFVFHILFIVFVFTSPTIFGQKTATITGKIIDEEHKPIVGASI
                     AILGKNVGTISNETGFFSLKITSNKVISVVFSFTGYVPVQKNMVLNTGETDFITIQLK
                     KDTATLLEVEVKDDRKKREPGSIHIDPSKAGVNPSPIQGIEQLIKIFVGSNNELTSQY
                     SVRGGSYDENLIYVNDFEVFRPYLIRNGQQEGLSFINPELTSGIKFYNGGFASKYGDK
                     MSSVLDITYKKPKQFGGSVYIGLLEQGLHIEGNNKKNNLSYLLGVRNRSNRNLLSSQE
                     TKGNYIPSSSDVQALINYRINNKWQVELFGNLSASKFTLYPEESKLTSSIFSPFYTAS
                     FGLDIFFEGKEKDNYSTNFIGFSATNQPKKNLKLKWLLSRFNNN"
BASE COUNT         1405 a          607 c          686 g         1438 t
ORIGIN      
        1 gtaaagaaga agttaaaggc ttttacattt cgccagctaa tgcattttta accaatagag
       61 tagatgctgt tattggctct ttatggaaag tgcctgatga aacaaccaat ttattacttc
      121 aagaatttta tactaatatt gaaaaaaagg gtatgacaaa agtagaagca ttacgccatg
      181 ctcaggcagc agttagcgaa aatcctaaat atagtcatcc gtttttttgg agtgcctttg
      241 ttttatacgg cgaatggaga taaaataaat tagctataat aaaattgcat agcttatttt
      301 cattcaaatt attttatgca atcaaaacag caacttggtt ttttttcact aaccatgata
      361 gtgataagct ttgtgatagg tatgggtatt tttaaaacat cctctaccgt tgcagcaaaa
      421 gcaggtaccg aaactatttt ttatgcagca tggttaatag ggggttttat tgcaatttgt
      481 ggggctttaa cgtatgcaga aattggcgtt aggctacctg ttacaggtgg gtattataaa
      541 atttttgctt atgcttatca tccttctatt ggttttacaa ttaacatttt agtgcttata
      601 agcaatgcag catctttggc tgtagtggca ctaattggtg ccgattatgt aagtgattta
      661 ttgtttgcaa aaccttctgg aactttattt aatattggta ttgcagcttt agctattttt
      721 ttattttatg gtgtaaattt attgggctta caaacaagta gtaaaacaca aaatgtgtta
      781 acaattttaa aagttgcatt agttgtatta cttatttcat ctttcttttc taatacacag
      841 gtagaaccac atggttatac taataataca atatatacat acaatggcaa caatggtgca
      901 ctattgctta tacttagctt agtggctgtt tcctttactt atggtggcta ccaacaaaca
      961 ataaactttg gagaggaagt gaagaatact cgtaccatgc aacgtagcat tattacagga
     1021 atgtttattg tagtgctatt gtattttgct attaattatg tttatgtgca tgtaattggt
     1081 ttcgataaaa tgaaaaatgc tacagctatt ggtgcattat taactaaagc atggtttgga
     1141 aatattggtg ctaaaatttt tgatgcctgt atgtttttaa gtgtattggc ttatgtaaat
     1201 gtagttttat taagtaaccc aagggtaatg tatgcaatga gtaacgatgg tgttttgcct
     1261 aaaatatttt caaataaaaa taataaaaca ggtgtattaa tacctggctt aactgtattt
     1321 gctttaactg cactaatcat tacttttttt ggaaaaggtg tagatgatgt attaagcttt
     1381 agtatttttt tagattgttt tggtatgagt ttatctgcag ctactttact tattttaaga
     1441 aaaagaaaac aaggcgaaga gaatgtaaaa ggatttttga aaaacatcac tcctgtttta
     1501 tgcatccttt ttgtaattgc gtatagcata gttgcagttg cagttgtaat tgataagcca
     1561 atggctgctt taactggtgt aattttatta gctgtattta tcctagttta tttcttgttc
     1621 tgccataaaa aacaaacagt aaaacttcct taacagcata aaaaacaaaa ccccgaaaat
     1681 tcggggtttt gaatattagt gaaaatataa ttattgtttt acaaattgta cagagcttct
     1741 tgtgccgtta ttttcataca ccataatata attaccattt aataatttac ttacatctat
     1801 tgtagtttgt gttgcaccat tttgtacgta cactacatta actgttttgc ctcctaaagt
     1861 agtaatttta atagttgctc tatctgtagc tttatcatga gataaagttg tagtatttct
     1921 aacagggtta ggatataaat ctaacttaac agttgtttta ccatttaagc ttactgtttt
     1981 gctgtattta aatgtaccat ctttatctat catttttaaa cgataataag aaattccatt
     2041 taactcgtta gtaaagctat aagcgttttc tgttttgtta tttgctttta caaagccaac
     2101 acttgtaaaa tctttaccat tattgctttt ttcaatatca aatccatcta catttacctc
     2161 attagtggta aaccaattaa gattagcttt accattaact gccgatgcag taaaagaatt
     2221 taaagtaagt ggtgcaagtg ctataccacc aatataaaaa ttatcaattc tagaagtgcc
     2281 tcctgttgca atagcagcac cattaatatt tgtgctacct gcagcaactg ttaatctaat
     2341 ataaatagca ggctggttat tcaacgctgt aattgcagct aaacttaaat tacgagtaga
     2401 aactggttta taggtacttg cagaccatgc atcatttgca atatcataac cagaacttgg
     2461 tacatcagta aacgttacac catctgtact gtattgtact aaaaaagatt taggacccgt
     2521 gttgctcccc atttggtcat aaactattgt aatagaatta aaacctgtgg tagatgcttt
     2581 gaattgccaa taatcgccag atgcccagcc actactactt gcagaattag cagaaccatt
     2641 acccgctgga gtaacccaag tagaagtagc agcagcatgg ttggcagaaa attctgagcc
     2701 tgttactaaa gcaccagaat ctgcaactac agaaccagca ccaacagtaa aggtattacc
     2761 tgttgcagaa gaaaaatcta atacctcaaa gttccatctt gcaattacct cttgtgcaag
     2821 caggcttgta gaaaaaaagg tgaataacaa cgaaagcgta aatattcttt tcataaaatt
     2881 tattttagtt gccacaaaat aagcaataaa aattaattca acatatattt ttattaaaaa
     2941 ttaatcttag gttaatatta aaatggtaca atacttatct tcattgcttt aaaagtactt
     3001 aatgaagttt gttttccata tattatttat agtttttgta tttacttctc ctacaatttt
     3061 tggccaaaaa acagctacca taactggtaa aattatagat gaagagcata aacctattgt
     3121 tggtgcaagt attgcaattc ttggaaaaaa tgtaggaact attagtaacg aaactggttt
     3181 tttcagctta aaaattactt ccaataaagt aataagtgta gtgttttctt ttacaggtta
     3241 tgtgccagta caaaaaaaca tggtattaaa taccggcgaa accgatttta ttactattca
     3301 attaaaaaaa gatactgcaa cacttttaga ggtagaagta aaagatgata gaaaaaaaag
     3361 agaaccaggc agcatacata tagatccatc taaagcaggt gttaaccctt cgcccattca
     3421 aggaattgaa caattaatta aaatttttgt aggtagtaat aacgaattaa cttctcaata
     3481 ctctgtacgt ggtggcagtt acgatgaaaa tttaatttat gtaaacgatt ttgaagtgtt
     3541 tagaccttat ttaataagaa atgggcaaca agaggggtta agttttatta atcctgaatt
     3601 aacaagtggc attaaatttt acaatggtgg ctttgcctct aaatatggcg ataaaatgag
     3661 tagtgtatta gatattactt ataaaaaacc aaagcagttt ggtggctcgg tttatattgg
     3721 tttattagaa caaggtttgc atatagaagg taacaataaa aaaaataatt taagttattt
     3781 attgggtgta agaaacagaa gcaatagaaa tttactaagc agtcaggaaa caaaaggaaa
     3841 ttatataccc tcctcgtccg atgtgcaagc attaattaat tatcgcataa acaataaatg
     3901 gcaggttgag ttatttggca atttatctgc ttcaaagttt actttatatc ctgaagaaag
     3961 taaattaacc agttccatat tttctccttt ttatactgct agttttggtt tagatatatt
     4021 ttttgaagga aaagaaaaag ataattactc caccaatttt attggctttt ctgccacaaa
     4081 tcaaccaaaa aaaaatttaa agttaaaatg gctgcttagc aggtttaata acaacg
//
LOCUS       DAOQHP010000192         4115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13063_length_4115_cov_3.853941, whole genome shotgun sequence.
ACCESSION   DAOQHP010000192 DAOQHP010000000
VERSION     DAOQHP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13063_length_4115_cov_3.853941"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLY81_06880"
     CDS             <1..407
                     /locus_tag="PLY81_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29988.1"
                     /translation="EDFDIIDSGEEIIDNTITRKIILLPKNETNDVALSTMYIDAEKL
                     LVKKVVTTTKENGTYQILISYGNYSQYGLPDKVVFGFNTKDYKMPKGITMEFDDEGKK
                     EMREKMKNKKGKVEIMYNTYIINKGINDAIFN"
     gene            439..1632
                     /locus_tag="PLY81_06885"
     CDS             439..1632
                     /locus_tag="PLY81_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNF29989.1"
                     /translation="MNNRQIFQQHLAQTSPAPIAIEMNKAEGIYLFDVDGKKYIDAIS
                     GFSVANIGHTNSTVVEAVTKQAKEYMHLIVYGEFIQQPQTAYAKLLTDNLPSDLNCVY
                     FTNSGTEATEGALKLAKRATGRTKIYAFNNAYHGSTQGSLSVIGSEYWRNNFRPLLPE
                     VYHFNYNSNEIIEAIDSTVACVIIEIVQAEAGIKAANKTWLQTLYKKCKANGVLFIVD
                     EIQSGFGRTGSLWAFEQFEIIPDILLLGKALGGGMPLGAFIANYSLMNLLTNNPVLGH
                     ITTFGGHPISCVAGKAALEVLLTENLLNNIQHKQSILINNLTKKLIKHVNYIGLWAAI
                     AFENFETNHAVIQKCITKGLITDWFLFAPNCMRLAPPLISTNQQLLDICSIINESIDE
                     VELNQ"
     gene            complement(1640..2200)
                     /locus_tag="PLY81_06890"
     CDS             complement(1640..2200)
                     /locus_tag="PLY81_06890"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNF29990.1"
                     /translation="MKANDKSFLVYVAKLVLLFLALDYGTTFFIGITTPGGSHYVPWL
                     DKYFNYVNWLRQTILQSSKFCMGILGYNMELVSPYILKIKNGAGVHLVYSCLGYGVIS
                     FWIAFVLANKATLWLKVKWLLAGVAIIIFTNILRVCILIIALQKKMFKPFTLDHHTFY
                     NIVAYSVVIILMLLFIKQIRKQQLKV"
     gene            complement(2220..2660)
                     /gene="smpB"
                     /locus_tag="PLY81_06895"
     CDS             complement(2220..2660)
                     /gene="smpB"
                     /locus_tag="PLY81_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNF29991.1"
                     /translation="MTKEMNNRQAYFNYFIEDKYVAGIVLLGTEVKSIREGKVSFNDA
                     FCLLVDGQLWLRGFYIAEYSHGTVNNHIAVHDRKLLLQKREIKKLQNKLKDKGLTIVP
                     LKVYLNEKNLVKVEIGLAKGKKLHDKRDTIKQRDAERELKRFVK"
     gene            complement(2684..3820)
                     /locus_tag="PLY81_06900"
     CDS             complement(2684..3820)
                     /locus_tag="PLY81_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF29992.1"
                     /translation="MNKHLHIISFTVPYPPNYGGVVDLFWKLPALQKAGIQIHLHCFE
                     YGQNLQTELNKYCASVHYYKRLVGLNGLSFTLPYIVASRKNEELFLKLLEDDYPILLE
                     GIHSTYLLNDNRFENRKIIVRLHNVECNYYKHLANNSNNLLNKLYYLAEHLLLKKYEK
                     KLATTKAIFLTVTEKDKSTFIHQFGYKNINYLPLFLPESWKFNLATTKNSYCLYQADL
                     SVVNNEKAAIWLLEKVFNTIDFPLIVAGKNPSLKLIQIVKKYKHVTLNANPNNEVMQQ
                     LISHAQINILPSLSNTGIKLKLLNALYNGKYCLVNSNTIDGTEFESLCIVANNVEEMQ
                     QKIKELFCMSFNEPEIIKRKTVLDKIYNNTQNAEQLIKYIFDES"
     gene            complement(3875..>4115)
                     /locus_tag="PLY81_06905"
     CDS             complement(3875..>4115)
                     /locus_tag="PLY81_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF29993.1"
                     /translation="MNEGKATPQQVSMAKRNSCEIATNIARDARQMLGGMGITGEYSI
                     MRHMMNLESVITYEGTHDIHLLITGMDVTGINAFK"
BASE COUNT         1455 a          625 c          565 g         1470 t
ORIGIN      
        1 tagaggattt tgatattata gatagtggtg aagaaattat tgacaatact ataacaagaa
       61 aaataatatt attacccaaa aatgaaacaa acgatgttgc gttaagtacc atgtatattg
      121 atgcagaaaa attattagta aaaaaagtag ttacaacaac aaaagaaaac ggcacttatc
      181 aaattttaat aagttatgga aattatagcc aatatgggtt acccgataaa gttgtttttg
      241 gttttaatac taaagattac aaaatgccta aaggcataac aatggagttt gatgatgaag
      301 ggaaaaaaga aatgagagaa aaaatgaaaa ataaaaaagg aaaagttgaa attatgtata
      361 acacatacat tataaataaa ggcattaatg atgccatttt taattaactt ttgctatatt
      421 attataaaat aactggtaat gaataacagg caaatctttc agcaacatct tgcacaaaca
      481 tctcctgcac caattgcaat agaaatgaat aaagccgaag gaatttattt gtttgatgtt
      541 gatgggaaaa aatatattga tgcaatttct ggatttagtg tagccaatat aggacacaca
      601 aattctacag ttgtagaagc tgtaaccaag caagcaaaag aatacatgca ccttattgta
      661 tatggtgagt ttattcagca accacaaact gcctatgcta aattactcac tgataattta
      721 ccttcagatt taaactgtgt ttactttaca aatagtggaa cagaagcaac agaaggtgct
      781 ttaaaattag caaaaagagc aaccggcaga accaaaattt atgcattcaa caatgcttat
      841 catggaagca cacaaggctc tttaagtgtt attggaagtg aatattggag aaataacttt
      901 cgtcctttgt tgcccgaagt ttaccacttt aattataact ctaatgaaat tattgaagca
      961 atagactcta ctgtagcttg tgtaattata gaaatagtgc aagctgaagc tggtattaaa
     1021 gctgccaaca aaacttggct tcaaacattg tataaaaaat gcaaagccaa tggtgtactt
     1081 tttattgttg atgaaataca aagtggtttt ggaagaacag gaagtttatg ggcttttgaa
     1141 caatttgaaa taatacctga tattttactt ttaggaaaag cattgggtgg tggtatgcct
     1201 ttaggtgctt ttattgccaa ctactcttta atgaatttat taaccaataa ccctgtatta
     1261 ggccatatta ccacctttgg tgggcatcct ataagttgtg ttgcaggtaa ggctgcttta
     1321 gaggttttat taacagaaaa tttattaaat aacattcagc acaaacaatc aattcttata
     1381 aataatctta caaaaaaatt aattaagcat gtaaattata ttggtttgtg ggctgctatt
     1441 gcttttgaaa attttgaaac taatcatgcc gttattcaaa aatgcataac aaaaggctta
     1501 attacagatt ggtttttatt tgctccaaac tgtatgcgtt tggctcctcc attaattagt
     1561 accaatcaac aattacttga tatttgtagt attattaatg aaagtattga tgaggttgaa
     1621 cttaatcaat aaatctaatt tatactttta gttgttgctt tcttatttgc ttgataaaaa
     1681 gtaacatcaa aataattact acagagtaag caacaatatt ataaaatgtg tgatgatcta
     1741 aggtaaatgg cttaaacatt ttcttttgta atgcaataat taaaatacat actcttaaaa
     1801 tattagtaaa aataattatt gcaaccccag ccaataacca ttttactttt agccataaag
     1861 tagctttgtt agctaataca aatgctatcc aaaaactaat aacaccatag cccaagcatg
     1921 aataaactaa atgtacacct gctccatttt ttatttttaa aatataaggg ctaacaagtt
     1981 ccatattgta tcccaatatg cccatacaaa acttagaaga ttgtaatata gtttgcctaa
     2041 gccaattaac ataattaaaa tacttatcca accaaggcac ataatggctt cctccaggtg
     2101 ttgtaatacc aataaaaaaa gtagtgccat aatctaatgc taaaaataac agcaccagct
     2161 ttgcaacata aactaaaaat gatttgtcat tagccttcac tataataaat tttgagttgt
     2221 tattttacaa aacgctttaa ttcacgttca gcatctcttt gtttaatggt atctctttta
     2281 tcgtgtaatt ttttgccttt cgctaaacca atttctactt ttaccaaatt tttttcgttc
     2341 aaataaactt taagaggcac tatggttaaa cctttatctt ttaatttatt ttgtagcttt
     2401 ttaatttctc ttttttgtaa aagcaatttt ctatcgtgta cagcaatatg gttgtttaca
     2461 gtaccatgtg aatattctgc aatataaaaa cctcttaacc ataattgccc atcaaccaac
     2521 aaacaaaatg catcattaaa acttactttt ccttctctaa tactttttac ttcagtgcct
     2581 aataatacaa tacctgcaac atacttatct tctatgaagt aattaaaata agcctgccta
     2641 ttattcattt cttttgtcat ggtattaata ctgcttggtt gcctcaactt tcatcaaaga
     2701 tatattttat taattgttct gcattttgtg tgttattgta tattttatct aatacggttt
     2761 ttcgttttat tatttccggt tcattaaaac tcatacaaaa taactctttt attttttgct
     2821 gcatctcttc cacattattg gcaactatac ataaactttc aaattcagtc ccatctattg
     2881 tattactatt taccaagcaa tatttgccat tataaagtgc atttaataat tttaatttaa
     2941 tacctgtatt acttaaagag ggtaaaatat taatttgtgc atggctaatt aattgctgca
     3001 ttacttcgtt gtttgggtta gcatttaaag taacgtgttt atattttttt acaatttgaa
     3061 ttagttttaa tgatggattt ttgcctgcaa caattaaagg aaaatcaata gtattaaata
     3121 ctttctccaa taaccaaatt gcagcttttt cgttattaac aacacttaag tctgcttggt
     3181 ataaacaata agaatttttt gtagttgcta aattaaattt ccaactttct ggtaaaaaca
     3241 aaggcaaata attaatattt ttatacccga attgatggat aaaagttgac ttatcttttt
     3301 ctgttacagt taaaaaaata gcctttgtgg tagctaattt tttttcgtat ttttttaata
     3361 ataaatgctc ggctagataa taaagtttat ttaataagtt gttgctatta ttagctaaat
     3421 gtttataata attacactct acattgtgta atcgtacaat aatttttcta ttctcaaatc
     3481 tgttatcatt caataaatag gtactatgaa taccctctaa caaaatagga taatcatctt
     3541 ctaataattt caaaaataat tcttcgttct ttctagaggc aacaatgtaa ggtaaagtaa
     3601 agcttagccc atttaagcca actaatcttt tataatagtg aacagatgca caatatttat
     3661 ttaattctgt ttgtaagttt tgtccatatt caaaacaatg taaatgtatt tgaatacctg
     3721 ctttttgtag tgctggtaat ttccaaaaca aatcaactac cccgccataa ttaggtggat
     3781 aaggaactgt aaaagaaatt atatgcaaat gtttgttcaa atacttactg ttgaatacag
     3841 tgcaatattc ttaaaataat ttagaaatag tttattattt aaatgcatta ataccggtta
     3901 catccatacc agtaattaac aagtgaatat catgagtgcc ttcgtaagta attacacttt
     3961 ctaaattcat catgtgtctc ataatactat attctcctgt aatgcccata ccaccaagca
     4021 tttgcctagc atctctggca atattcgtag caatttcgca gctatttctt ttagccatac
     4081 ttacttgttg tggcgttgct tttccttcgt tcatt
//
LOCUS       DAOQHP010000193         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13155_length_4098_cov_4.879792, whole genome shotgun sequence.
ACCESSION   DAOQHP010000193 DAOQHP010000000
VERSION     DAOQHP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13155_length_4098_cov_4.879792"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /locus_tag="PLY81_06910"
     CDS             complement(<1..2192)
                     /locus_tag="PLY81_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29994.1"
                     /translation="MIWNLIIQRYTDIRSDERKPVSILFLHSLLLGFSTSLFFVAANS
                     YFIKNVKINNIPYAYIAAGILGILLVFLFKYLIKVIGSIFTFFTVLVLFAITCFALYI
                     GHLYLDSTSLLSLVLAFLGFIFIFPFSGLFVLGFTGICLQLFNLHQNKRLLALIGVGE
                     VVASIIGYLMVPIITKFTGNTVHLFLFSGIFILASTRPIWHIYKINIKTFSVTPGSKT
                     IKRNYVGIFKKDRFYRSLAIVTFFSVLAVYFSDYAYLISVRKLAQLTSMDVANIVAVL
                     FCIIKIGELLFSFFSAKIISTIGMKFSLLLLPFMLILFSTLSILAFSMFKEVLIFVIL
                     FFLMSKWCDRVLRKGVYAPSSKVMYQVVEPVERMQLQTTIEGTISQFSIVLSGLILLL
                     VSKLKLTTNNDVLFYSLTAISALFSICWFVMAWLLFNNYKTKIHDYLHNINGIKNTVQ
                     KQNTSILINQWVNNTEFGLSIEDKNSILQVYDNVQMDTVNMQKIMEHIAFYNPSLQSF
                     LANNDSTLLHKKIVRLYFTNDHFFSRVLIIKYLQQNNQLSDFNIFYELYEAADFEIKK
                     IIISSLNQLNYTTQNNQYYFYDLCKIFVQEIIWAELSIYDTINVPNHHLHTLLQEYAT
                     ECKNHLLNILKVLYGNNVITIVQQVLNENDKAAEGSIFAVELLDNILQQPLKEKIIPV
                     FEPIPQQMRWAKLGTFCNIYHLSEEQRLKEILIKNFNLINIKLKQTAL"
     gene            complement(2207..2761)
                     /locus_tag="PLY81_06915"
     CDS             complement(2207..2761)
                     /locus_tag="PLY81_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF29995.1"
                     /translation="MKICFQINNIIAEVEIKQPLISDIPYLQNLNSKWQFNALNGNTE
                     KGFLSGLFDEAFFRTLIKEEAIIAAYSNNTLIAYMLTANNANLNILKTHKEEVEKLKN
                     NLVINKNAKVAIGIQTAVEEDYHGSGLIVAIRNEFRKMLCNKFQYLFTTISKNNQRSF
                     ASATKYGWKKVGEDEHHYFLLLTV"
     gene            complement(2774..3667)
                     /locus_tag="PLY81_06920"
     CDS             complement(2774..3667)
                     /locus_tag="PLY81_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNF29996.1"
                     /translation="MKNIKSEKAVYKIVKAYAFTAIDKIENKKVIGNPASIVICNKRF
                     PPQTQMQQMAVKEKQPMTAFVLHEKENNFSIKYFTPLGIEFGLCVHATLIASSVVQQE
                     FGYTNIHFNAHKISLSLTLSNSFLSTNHTVAQIKLPTYMPVLVNHKLPLHYLKIIGVE
                     QQDIKYVYKCDELKDEIIVLNNAEILRSVKPNFLELSKVLTTSNTRGLFLTSLSTIKN
                     IDYEVRIFAPHFGIDEDISCGSANCSLIPLWHKITNEVGNKEYKIICPYNGKDNVIGG
                     VELGVFDVENNSAFIGGIVTS"
     gene            complement(3686..>4098)
                     /locus_tag="PLY81_06925"
     CDS             complement(3686..>4098)
                     /locus_tag="PLY81_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF29997.1"
                     /translation="VLNYTYTNPKQTIDDAGNKANLTVADIAKHHINAIVNYTFLDNL
                     NINFRLNYISNKYAGVGTTVATNPQNVFPGYTIANSTIGYTIIKGTTVQLVCNNIFNK
                     YYFNPAGRAADGINTPSSLLQPGRNFFIKLNYEF"
BASE COUNT         1465 a          582 c          568 g         1483 t
ORIGIN      
        1 tctaaggctg tttgttttaa tttaatatta attaggttga aattttttat taaaatttct
       61 tttagccttt gctcttcact taaatgataa atattacaaa atgtacccaa ttttgcccaa
      121 cgcatttgtt gagggattgg ttcaaaaaca ggaataattt tttcttttaa aggttgttgt
      181 aaaatattat ccaataattc aacagcaaaa atacttcctt ctgctgcttt atcattttca
      241 tttaaaacct gttgtactat agtaattaca ttattaccat ataacacttt taaaatattt
      301 aataaatggt ttttacattc tgttgcatat tcttgtaaca aggtatgtaa atgatggtta
      361 ggtacattta tggtatcata aatagaaagc tctgcccaaa taatttcttg aacaaaaatt
      421 ttacaaaggt cataaaaata atattggtta ttttgggttg tatagtttaa ttggtttaat
      481 gaggatatga ttattttttt tatttcaaaa tctgcagctt cgtacaattc ataaaaaata
      541 ttaaaatcac ttagttgatt attttgttgc aggtatttaa ttattaaaac cctactaaaa
      601 aaatgatcgt ttgtaaaata taatcgtaca atttttttgt gaagtaatgt actatcatta
      661 ttggctaaaa agctttgtaa ggatggattg tagaaggcaa tatgctccat tattttttgc
      721 atatttactg tatccatttg tacattatca taaacttgaa gaatactatt tttatcttct
      781 atacttaaac caaactcagt attgttaacc cattggttaa ttagtataga tgtattttgt
      841 ttttgaactg tatttttaat gccattaata ttatgtaaat aatcatgaat tttggtttta
      901 taattattga ataacaacca ggccattaca aaccaacata tagaaaataa agcagaaata
      961 gccgtaaggg aataaaataa tacatcgtta ttagtagtta atttaagctt tgaaaccaat
     1021 aataaaatta atccgcttaa tacaattgaa aattggctta ttgtaccttc aatagttgtt
     1081 tgtagttgca ttctttctac tggctcaact acttgataca ttactttaga agaaggagca
     1141 tacacccctt ttcttaaaac cctatcgcac catttcgaca ttaaaaaaaa gaggattaca
     1201 aaaatcagca cctctttaaa catactaaaa gcaagtatag acagagttga aaataaaatc
     1261 aacataaaag gcaatagcaa taaggaaaac ttcatgccta ttgtgctaat aatttttgca
     1321 gaaaaaaatg aaaagagtaa ttctccaatt tttataatac aaaaaagtac agcaacaata
     1381 tttgcaacat ccatacttgt taattgtgca agtttgcgta cagaaattaa gtatgcataa
     1441 tcggaaaaat aaactgctaa aacagaaaag aaagtaacaa ttgctaatga cctataaaac
     1501 ctatcttttt taaaaatgcc tacataattt cttttaatag ttttagaacc aggtgtaacc
     1561 gaaaaagttt taatattaat tttataaata tgccatatag gtcgggtaga ggctaaaata
     1621 aaaattccag aaaataaaaa taagtgcaca gtatttcctg taaacttggt aataatgggt
     1681 accattaaat atcctattat tgaagctaca acttcgccaa caccaattaa agccagcagc
     1741 cttttatttt gatgaagatt aaacaactgc aagcaaatac ctgtaaaccc taatacaaaa
     1801 agaccagaaa aaggaaatat aaaaataaag cccagaaatg ctaacaccag ggataataaa
     1861 gatgtagagt ctaaatacaa atgccctatg tataaagcaa agcaagtaat ggcaaataaa
     1921 acaagcacag taaaaaatgt aaaaatgctt cctataactt taattaaata tttaaaaaga
     1981 aaaacaagta aaatacctaa aatacctgca gcaatatatg catacggaat attgttaatt
     2041 tttacattct taataaaata actattagca gcaacaaaaa ataaggaagt agagaagcct
     2101 aatagtaaag agtgtaaaaa taaaatagat acaggttttc gttcatctga acgaatatcg
     2161 gtataacgct gtattattaa attccatatc atttattaat aaaattctaa acggttaaaa
     2221 gtaaaaaata atgatgctcg tcttcgccca cttttttcca accatatttt gttgcagagg
     2281 caaatgatcg ttggttattt tttgaaatgg ttgtaaaaag gtattgaaac ttattacaca
     2341 gcatttttct aaattcattt ctaatagcta ctattaatcc actgccatga taatcttctt
     2401 caactgctgt ttgaatacct attgcaacct ttgcattttt attaattact aagttattct
     2461 ttaatttttc aacttcttct ttatgtgttt ttagtatgtt taagtttgca ttattagctg
     2521 taagcatgta tgcaattagt gtgttgttgc tgtaggctgc tataattgct tcctctttaa
     2581 tgagtgttct aaaaaaagcc tcatcaaata aacccgacaa gaatcctttt tctgtatttc
     2641 cgtttaacgc attaaactgc catttgctat taaggttttg caaataaggt atatctgaaa
     2701 ttaatggttg tttaatttca acttcagcta ttatattatt tatctgaaaa caaattttca
     2761 ttgttccata aaattaactt gtaacaatac ctcctataaa agcactattg ttttctacat
     2821 caaaaacgcc taattctact ccacctataa cattatcttt tccattatat gggcaaatta
     2881 ttttgtattc tttattgcct acttcattag ttattttatg ccataaagga attaatgaac
     2941 aattggcaga gccgcaagag atgtcttcat ctattccaaa atgaggtgca aaaattctta
     3001 cttcataatc tatatttttt atagtagata aagaggtaag aaacaatcct cttgtattgg
     3061 atgtagttaa aactttactc aactccaaaa aatttggttt aacacttctt agtatttctg
     3121 cattatttaa aactattatt tcatctttca gttcgtcgca tttgtacaca tattttatat
     3181 cttgctgttc aactcctata atttttaagt aatgtaaagg tagtttatgg tttaccaata
     3241 caggcatgta agtaggcaat tttatttgtg caactgtatg atttgttgac aagaaactat
     3301 tgcttaatgt aagagacaaa gaaattttat gagcattaaa atgtatattg gtatagccga
     3361 actcttgttg tactacactt gatgcaataa gtgttgcatg tacacatagg ccaaactcta
     3421 tacctaatgg tgtaaaatat tttatagaaa aattattttc tttttcatgt aaaacaaatg
     3481 cagtcatagg ttgtttttct ttaacagcca tttgttgcat ttgggtttga gggggaaacc
     3541 ttttattaca aataacaata cttgctgggt tgccaattac ttttttattt tctattttat
     3601 ctatagcagt aaatgcgtaa gctttaacta ttttgtaaac tgctttttca gatttgatat
     3661 ttttcataat caaactcatg ttaaattaaa attcataatt taacttaata aaaaagtttc
     3721 tacctggttg taataaacta gatggtgtgt taataccatc tgcagccctg cctgctgggt
     3781 taaaataata tttattaaaa atattattac aaactagttg tactgtggtg cctttaatta
     3841 tggtgtaacc aatagtactg ttagcaatgg tatagcctgg aaaaacattt tgagggttgg
     3901 ttgcaactgt tgtgcctaca cctgcatatt tattacttat ataatttaag cggaagttta
     3961 tatttaggtt atctaaaaaa gtataattaa caatagcatt aatatggtgt ttggctatat
     4021 ctgcaacggt taggttggct ttattgcctg catcatctat ggtttgtttg gggttagtat
     4081 aagtatagtt taaaacaa
//
LOCUS       DAOQHP010000194         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13260_length_4079_cov_3.225398, whole genome shotgun sequence.
ACCESSION   DAOQHP010000194 DAOQHP010000000
VERSION     DAOQHP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13260_length_4079_cov_3.225398"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLY81_06930"
     CDS             complement(<1..508)
                     /locus_tag="PLY81_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNF29998.1"
                     /translation="MKVFKDLNQLLAFNNAVITIGTFDGVHEGHKKILKQLKEQAITV
                     NGESIVITFYPHPRKVVGDTTGVLLLTTLEEKIELLKKELIDNVVVVEFNEDFANQTA
                     EEYISDFLFKKFKPHTLIIGYDHHFGYKRKGNYELLEKFGQKLGFSVKEIDEKLLSET
                     AISSTRIRK"
     gene            610..1779
                     /locus_tag="PLY81_06935"
     CDS             610..1779
                     /locus_tag="PLY81_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNF29999.1"
                     /translation="MSLYNQRGVSAQKEEVHNAIKQLDPGLYKNAFCKMYPDFLLGDE
                     NFVNISHADGAGTKSILAYLYWKETNDVSIWKGIAQDAVAMNLDDLLCVGVYDNFLFN
                     STIDRNKSKIPADVLEAIINGTQTFFNELKNYGINIHYLGGETADVGDVVRTIAVNGT
                     MTTRWPKNKLITNDKIDAGNVIVGFASYGKATYETAYNSGLGSNGLTSARHDVLSKYY
                     AEKYPETFEHSLSNNVVYIGKNKLTDTVQVENENIEIGKLLLSPTRTYAPLMKLLLKE
                     NFEDIYGVIHCSGGGQTKCLKYLPKPLLVIKNNLFDAPPIFKLIQENSNADFKEMYQV
                     FNMGHRLEIFTNETAAHKMIATANTLGIEAKIIGYTEQANKSKLILNGSFGEICF"
     gene            1922..3205
                     /locus_tag="PLY81_06940"
     CDS             1922..3205
                     /locus_tag="PLY81_06940"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNF30000.1"
                     /translation="MSTISKNTVAVILGGGAGTRLYPLTAARSKPAVPIAGKYRLVDI
                     PISNCINSNINRMFVLTQFNSASLNKHIKNTYHFSAFSTGFVDILAAEQTPDNQAWFQ
                     GTADAVRQSLRHIDTLDFEYVLILSGDQLYQMDFASMINEHKESNSDISIATIPVHQR
                     EAQEFGIMKTNTENVITSFVEKPKLDVLANWVSDTGEQMKLEGRNYLASMGIYVFNRK
                     VLFDLLQNVHPSATDFGKEIIPNSIKNYKVTSYQYDGYWTDIGNIYSFYEANLSLTLE
                     IPMFNLFDNTKYIYTRPRMLPPAKISGTQLDKALIAEGCIIHASKIENSVVGIRARIG
                     AGTTIVNSYIMGSDYYETIEEMAKHLIQGIPKIGIGDNCQIKDVIIDKNCRIGNNVVI
                     NGGKHLENKEHELYVIKEGIVVIKKGAIIPDGFVI"
     gene            3369..>4079
                     /locus_tag="PLY81_06945"
     CDS             3369..>4079
                     /locus_tag="PLY81_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNF30001.1"
                     /translation="MEITAKTAQQLRLTEEEFELIKQKLGRTPNFTELCAFSAMWSEH
                     CSYKNSIKWLKTLPRSGGRMLVAAGEENAGLMDMGNGFGVVFKIESHNHPSALEPFQG
                     AATGVGGIQRDIFTMGARPIASLNSLRFGNLQDKKTQRLLNGVVYGIGHYGNCFGVPT
                     VAGEIYFEDCYHTNPLVNAMSVGIMQADKMISATAQGEGNPVFFVGSATGKDGIGGAS
                     FASADITNESAQELPAVQV"
BASE COUNT         1484 a          542 c          721 g         1332 t
ORIGIN      
        1 cttttctaat tctggtactg cttatagctg tttctgaaag cagtttttca tcaatttctt
       61 ttacactaaa acctaatttt tgtccaaatt tttcaagtaa ttcataatta ccttttcgtt
      121 tataaccaaa gtgatgatcg tagccaataa ttaaagtgtg tggtttaaat tttttaaata
      181 aaaaatctga tatatactcc tctgcagttt ggttagcaaa atcttcatta aactccacta
      241 caacaacatt gtctatcaat tcttttttaa gtaattctat tttttcttct aaagtggtaa
      301 gtaataaaac acctgttgta tcgccaacaa cttttcttgg atgagggtaa aaagttatta
      361 caatactttc tccattcaca gtaatggctt gttcttttaa ctgttttaat atttttttat
      421 gtccttcatg cactccatca aaagtgccaa tggtaataac agcattatta aatgcaagta
      481 attggtttaa atctttaaat accttcatat ttacaaaaat agtgaatggc aaataggata
      541 catagattta cttttgcaaa caattaatta ctttaaagca agtttttagt agagcttaaa
      601 aataccatta tgtcattgta taatcaaaga ggagtatctg cacaaaaaga agaagtgcat
      661 aatgcaataa aacaattaga tccagggcta tataaaaatg ctttttgcaa aatgtatcca
      721 gattttttac ttggcgatga aaattttgta aatatttctc atgccgatgg tgctggtaca
      781 aaaagtattt tggcttattt atattggaaa gaaactaatg atgtaagcat ttggaaaggt
      841 attgctcaag atgcagttgc aatgaattta gatgatttac tttgtgtcgg tgtgtatgat
      901 aattttttat tcaattctac catagataga aataaatcta aaatacctgc agatgtttta
      961 gaagcaatta ttaatggtac acaaacattt tttaatgaac taaaaaacta cggaattaat
     1021 attcattatt taggaggaga aacagctgat gtaggagatg tggtaagaac tattgcagta
     1081 aatggcacca tgactactcg ttggcctaaa aataaattaa ttactaacga taaaatagat
     1141 gcaggaaatg taattgttgg ctttgcatct tatggtaaag ctacttatga aactgcttat
     1201 aacagtggat taggaagtaa tggcttaacc agtgcaaggc atgatgtatt aagtaaatat
     1261 tatgctgaaa aataccctga aacatttgaa cattctttaa gcaataatgt ggtgtacatt
     1321 ggtaaaaata aattaacaga taccgttcaa gttgaaaatg aaaatattga aattggtaaa
     1381 cttttattaa gcccaacacg tacctatgca cctttaatga aattattatt gaaagaaaat
     1441 tttgaggata tttatggtgt aatacattgt agtggtggtg ggcaaactaa gtgcttgaaa
     1501 tatttaccca aaccattatt ggttattaaa aataatttgt ttgatgctcc tccaatattt
     1561 aagttaatac aagaaaactc taatgcagat ttcaaagaaa tgtatcaagt atttaatatg
     1621 gggcataggc tagaaatttt tacaaatgaa actgctgcac ataaaatgat tgcaactgca
     1681 aatacattag ggattgaagc aaaaattatt ggatatacag agcaagctaa caaaagcaaa
     1741 cttattttaa atggaagctt tggagaaatt tgtttttaaa agcatataaa attgcagcat
     1801 cttaaaactt caaattattt ttaaaaattg taggctttat tttttaaaat caatagataa
     1861 cagttaactt cataccatac atttggataa ttaaatatta aaactccaat gtacttgatg
     1921 tatgtcaaca attagtaaaa acacagtagc agttatactt ggtggtggcg caggtactcg
     1981 tttataccca ttaacagcag cacgtagcaa accagctgta ccaattgccg gaaaatatag
     2041 gttagtagat attccaatta gtaattgcat taatagcaat attaatagaa tgtttgttct
     2101 tacgcagttt aatagtgcat cactaaacaa gcatataaaa aacacatatc attttagtgc
     2161 attcagtaca ggctttgtag atattttagc agcagagcaa acgccagata atcaagcttg
     2221 gtttcaaggc actgcagatg ctgtaagaca aagcttaagg catattgata ctttagactt
     2281 tgaatatgtg ttaattctca gtggcgatca attgtaccaa atggattttg catctatgat
     2341 taacgagcat aaagaaagta attcagatat aagtatagca acaattccag tacatcaaag
     2401 agaggcacaa gagtttggaa taatgaaaac aaatactgaa aatgtaatta cttcttttgt
     2461 agaaaagcca aaattagatg tattagcaaa ctgggttagc gatactggcg aacaaatgaa
     2521 attagaaggt agaaactatc ttgcatcaat gggtatttat gtttttaaca gaaaagttct
     2581 ttttgattta ttacaaaatg ttcatccgtc tgcaacagat tttggtaaag aaataatacc
     2641 aaattcaata aaaaattata aagttaccag ctatcagtac gatggttatt ggacagatat
     2701 tggaaatatt tattcgtttt atgaagccaa tctttcttta acattagaaa ttccaatgtt
     2761 taatttattt gataatacaa agtatattta tacaagacca agaatgttgc caccagcaaa
     2821 aattagtggc acccaattag ataaagcgtt gattgcagaa ggctgcatta tacatgcaag
     2881 taaaattgaa aatagtgttg tgggtattag agcaagaatt ggtgctggaa caacaattgt
     2941 aaattcctac attatgggta gcgattatta tgaaacaatt gaagaaatgg ctaagcattt
     3001 aatacaaggt attcctaaaa ttggtattgg cgataattgc caaataaaag atgtgattat
     3061 tgataagaat tgtagaattg gaaataatgt tgttattaat ggtggcaagc acttagaaaa
     3121 taaagaacat gaattatatg tgataaaaga aggtattgta gtaattaaaa aaggtgcaat
     3181 tataccagat ggctttgtaa tataagtaga ataaaaatta tataaatttc aaattcaatt
     3241 gatattattt tttttaaatt ttattataga agataaatgg caattatcca tattatctta
     3301 ataatctttg ggctttaaat attgcttgct ttcttactta gcttacattt gctgaatata
     3361 attttacaat ggaaattaca gcaaaaacag cccaacaact tcgtttaaca gaagaagaat
     3421 ttgagttgat aaagcaaaag ttaggtcgta caccaaactt tacagaactg tgtgctttta
     3481 gtgccatgtg gagtgagcat tgtagttaca aaaattctat aaaatggctt aaaacattac
     3541 caagaagtgg tggcagaatg ttagtggcag caggcgaaga gaatgcaggc ttaatggata
     3601 tgggcaatgg gtttggtgtg gtttttaaaa ttgaaagcca taaccatcca agtgcattag
     3661 aaccttttca gggtgcagct acaggcgttg gaggtattca aagagatatt tttactatgg
     3721 gggccaggcc aattgcatct ttaaacagtt tgcgttttgg caatttacaa gataaaaaaa
     3781 cgcagcgttt attaaatggt gttgtatacg gtattgggca ttatggaaac tgttttggcg
     3841 tgccaactgt tgctggtgaa atttactttg aagattgcta ccataccaat ccattagtaa
     3901 atgcaatgag tgtgggaata atgcaagcag ataaaatgat tagtgccact gcacaaggag
     3961 aaggtaaccc agtttttttt gttggtagtg caaccggaaa agatggaatt ggtggtgcaa
     4021 gttttgcaag tgccgatata acaaacgaaa gtgcacaaga attacctgca gtacaagtt
//
LOCUS       DAOQHP010000195         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13291_length_4074_cov_4.014431, whole genome shotgun sequence.
ACCESSION   DAOQHP010000195 DAOQHP010000000
VERSION     DAOQHP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13291_length_4074_cov_4.014431"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PLY81_06950"
     CDS             complement(<1..1335)
                     /locus_tag="PLY81_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HNF30002.1"
                     /translation="MALPYLINHIYNNGSEEVVRRGKKIHQSGNVELIENNEFLGNLT
                     FRIKDDTYHTFYKVYITNFKDEKNIGTRCTCPYNLSEICRHKAGTLFYLQELLDKNLL
                     GSTSRAYNQQHTILKMKQLDLKFIKMLSSSKAFADAEEFLRSKKANIIEAKDECVLAE
                     VEWNNKTWKVIIRKNEERNFDTSCTDTSETNYPLCTQKIIVLLQLLNRYGSNYFDSIR
                     NWDAVKNKLLGIYGYTLSDDLTNKFEFTYKDGKPFLRVLDQNIKRIDAPLNAEIKPKP
                     SWKETYGETKNEPIETEETLKKNIEQNSTIKLGIVISNNNSQYPFFQIDAIQGEAAED
                     YSSYVGKVEKLDLNKFVDTTLFNEEDKMLFQQIRKLSTIELNKYLSRNSPFAGIWDNI
                     IQPNSNSFTVETKVLINEFFQPKLKNLFTDIAGSNFSFYLPDGKKFITAELEH"
     gene            1542..2144
                     /locus_tag="PLY81_06955"
     CDS             1542..2144
                     /locus_tag="PLY81_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNF30003.1"
                     /translation="MNYFTHLKKDKTLAICLLNEMPTLSFQKNIPLRLIAAIMSQQLS
                     TKVAKIIYQRFLDLYNGKVPSPQMVLDTPFATLRNIGLSNAKVNYVHNVATFCIENKI
                     TDKKLQKLSNEEIIELLIKIKGVGKWTVEMLLMFSLGREDVFSADDLGIQQAMIKLYK
                     LDTTNKKLLLANMQKIAEKWKPYRTYACLHLWRWKDTVKA"
     gene            complement(2134..2865)
                     /locus_tag="PLY81_06960"
     CDS             complement(2134..2865)
                     /locus_tag="PLY81_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF30004.1"
                     /translation="MEHLLTTESLISFLILVVLEIVLGIDNVIFVSIILNRISKNLQK
                     KARIMWIIAGIIFRCSLLLALQWLLDQKGKAIFTIYEKPFDLASLVMLAGGLFLMYKS
                     VKEIHHKVEGEEDATKQNNKPLSFTNAIFQIMIIDIVFSFDSIITAGGTAKHIEIMIA
                     AVVIAMVIMFIFSTNIANFIQQHPTIKMLALSFLVMIGLSLIIEGWNPVAAHDLHLKN
                     YIYFAMAFSFAVELLNMRMRKKVKL"
     gene            complement(2946..3572)
                     /locus_tag="PLY81_06965"
     CDS             complement(2946..3572)
                     /locus_tag="PLY81_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30005.1"
                     /translation="MKRITILFMSASLLLSFTVTQAQETGGKRRGKLEKREKMAGDKK
                     AGFGKDLNLTEEQKAEAKKINEEYNAKVKALKSNDKITMGEYNKQMAALQKERREKLE
                     AILTPEQKEKVESRKQDKEDKMQKREEMVLEKMKENLNLSDDQVTKIKTINETYAKKA
                     QAVRNDKNLSQEQKKQALLAIAKERKEAIGNTLTTEQKEKQKNFRNRD"
     gene            3751..>4074
                     /locus_tag="PLY81_06970"
     CDS             3751..>4074
                     /locus_tag="PLY81_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30006.1"
                     /translation="MRIAIYIFVLLLNCNFLFAQVDTSKIDTLKTQIKDTTKKKSKPL
                     VKPKTISDSILHITDTIISTQDSVKSNSPDSAIIKIAEPKKLLIDTSTFESLFSSKYY
                     PVNGSR"
BASE COUNT         1317 a          619 c          524 g         1614 t
ORIGIN      
        1 atgttctaat tcggcagtta taaatttttt tccatcgggt aaataaaaac taaaattact
       61 gccggctata tcagtaaata aattttttaa ctttggttga aaaaattcat taattaatac
      121 ttttgtttct actgtaaaac tgttgctgtt gggctgtata atattatccc aaataccagc
      181 aaatggagag ttgcgagata agtatttatt taactcaata gttgataatt ttctaatttg
      241 ctgaaaaagc attttatctt cttcgttaaa taaagtggta tccacaaatt tattcaagtc
      301 taatttttct actttcccta cataactact ataatcttct gcagcttcgc cttgaatggc
      361 atctatttga aaaaatgggt attgtgaatt attattggat attactatac ctaattttat
      421 ggtagaattt tgttctatat tttttttcag ggtttcttca gtttctatgg gttcgttttt
      481 agtttcgcca taagtttctt tccaagatgg cttaggtttt atttctgcat ttaaaggtgc
      541 atcaattctt ttaatatttt ggtctaatac tcttaagaaa ggctttccat ctttataagt
      601 aaattcaaac ttgtttgtta aatcatctga taaagtgtaa ccataaatac caagaagttt
      661 attttttact gcatcccagt ttctaatact atcaaaataa ttactaccat atctgtttaa
      721 taactgtaat aaaacaatta ttttttgtgt acataatggg tagttggttt cacttgtatc
      781 tgtacaactt gtatcaaaat ttctttcttc gttttttctt attattacct tccatgtttt
      841 gttattccat tctacttctg ctaaaacaca ttcgtctttt gcctctataa tatttgcttt
      901 tttacttctt aaaaattctt ctgcatcggc aaatgctttt gatgaagaca gcattttgat
      961 aaacttcaaa tcaagctgct tcatttttaa aattgtatgt tgttggttat aagctctgct
     1021 tgtagagcct agcaagttct tatctaataa ttcttgtaaa taaaataatg tacctgcttt
     1081 gtgtcggcat atttcagata aattataagg gcaagtacat cttgtaccaa tatttttttc
     1141 atctttaaaa ttggtaatat atactttata aaaagtatga taggtatcgt cttttattct
     1201 aaaagttaaa tttcctaaaa actcattgtt ttctataagt tctacattgc cagattgatg
     1261 aattttttta cctcttctta caacttcctc tgagccatta ttgtaaatgt gattaattaa
     1321 atagggtaga gccattgatg aaaaatgtgg attaatagaa aaggttaagt agcaaaaata
     1381 aaagaatgtt acccttattt tactctatac ttaatagttt gtggaagaaa tattttattc
     1441 agttattatt aaggattatt tgctaaaaag atgtaacttg tgccgttttt ttttatccat
     1501 aaattttgat atatactttt tcccatcact caattttaaa gatgaattat ttcacacatt
     1561 taaaaaaaga taaaactcta gctatttgtt tactgaatga aatgccaaca ctatcttttc
     1621 aaaaaaatat accattaagg cttattgctg ctataatgag ccagcagcta agtactaagg
     1681 ttgccaaaat aatttatcaa agatttttag atttatataa tggtaaagta ccaagccctc
     1741 aaatggtttt agacactcct tttgccactt taagaaatat tggattaagc aatgctaaag
     1801 taaattatgt acacaatgtt gctacttttt gtattgaaaa taagattaca gataagaaat
     1861 tgcagaaact gagcaacgag gaaattattg aattacttat taaaataaaa ggtgttggta
     1921 aatggacagt agaaatgttg ttaatgttta gcttaggcag agaagatgtt ttctctgcag
     1981 atgacttggg cattcaacag gctatgatta agttgtataa attagataca acaaataaaa
     2041 aactattact tgctaatatg caaaaaattg ctgaaaaatg gaagccttat agaacttatg
     2101 cttgtttgca tttgtggaga tggaaagaca cagttaaagc ttaacttttt ttcgcattct
     2161 catatttaaa agttctacag caaaggagaa agccatagca aaataaatat aattttttaa
     2221 atgtaaatcg tgtgctgcta ctgggttcca accttctata atcaaagata aaccaatcat
     2281 taccaaaaaa gatagggcaa gcatttttat ggttggatgt tgttgaataa aatttgcaat
     2341 attggtacta aatataaaca ttataaccat tgcaattaca acagcagcaa tcattatttc
     2401 aatatgtttt gcagtaccgc ctgcagtaat aatactatca aaactaaaaa caatatcaat
     2461 aatcattatt tgaaaaattg cgttagtaaa agatagtggt ttattatttt gcttcgttgc
     2521 atcttcttca ccctctactt tatgatgaat ttctttaacc gatttgtaca ttaaaaataa
     2581 accacccgca agcatcacta aactagctaa atcaaaaggt ttttcatata tggtaaaaat
     2641 agctttgcct ttttggtcta acagccattg caaagccaaa agtaaagagc atctaaaaat
     2701 tatacctgca ataatccaca ttattcttgc ttttttttgt aagttttttg agatacggtt
     2761 taaaattata cttacaaaaa ttacgttatc aatacctaaa acaatttcta ataccactag
     2821 tattaaaaaa ctaattaaac tttcagtagt caataaatgc tccattaata aattttatat
     2881 ttgcagcaaa taaataaaaa aaaccttaca atttgtaagg tttttttact tctaaatcta
     2941 acacctcagt ctctgtttct aaaatttttt tgcttttctt tttgttcagt tgttaaagta
     3001 ttacctattg cttcttttct ttctttagca atagctaata atgcttgctt tttttgctct
     3061 tgagataaat ttttgtcatt cctaacagct tgtgcttttt tggcataagt ttcattaatg
     3121 gtttttattt ttgtaacctg atcgtcggat aagtttaaat tttctttcat tttttctagt
     3181 accatttcct ctcttttttg cattttgtct tccttgtctt gttttcttga ttctactttt
     3241 tctttttgtt caggagttaa aatagcttct aatttttctc ttctttcttt ttgcaaagca
     3301 gccatttgct tattatactc acccatggtt attttatcat tgctttttag tgctttaact
     3361 tttgcattat actcctcatt aattttttta gcttcggcct tttgttcctc tgttaagttt
     3421 aaatctttac caaagccagc ttttttatca ccagccattt tttctctttt ctccaatttt
     3481 cctcttcttt taccacctgt ttcttgtgct tgtgttactg taaaacttag caataaactt
     3541 gcactcataa ataaaattgt aattcgtttc attgtttatt tttttattgt ttttaaatgg
     3601 tttgtataat catagacaag caattggttt aaaggtttaa aagcaaattt catatttagt
     3661 taaaattttg tttaagaaag ctttgctttt ttaaacaagt aagttttttt taggaacctt
     3721 ctctattaat tttgccaaaa atttctaagc ttgagaattg ctatatacat ttttgtgctt
     3781 ttacttaatt gcaattttct atttgcacaa gtagatacta gtaaaataga tactcttaaa
     3841 acccaaataa aagataccac taaaaaaaag agtaaaccct tagttaagcc taaaacaatt
     3901 tcagattcta tcttacatat tacagatact attatttcta ctcaagattc tgttaaaagc
     3961 aattcaccag attctgctat aattaaaata gcagagccaa aaaaattact catagacact
     4021 tcaacattcg aatcactttt tagttctaag tactaccctg taaatggtag caga
//
LOCUS       DAOQHP010000196         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13402_length_4055_cov_3.468250, whole genome shotgun sequence.
ACCESSION   DAOQHP010000196 DAOQHP010000000
VERSION     DAOQHP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13402_length_4055_cov_3.468250"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..3191
                     /locus_tag="PLY81_06975"
     CDS             141..3191
                     /locus_tag="PLY81_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30007.1"
                     /translation="MAENFMKHQLKINKTFLAIISCVYVFAQPNTDVDLYKEKPQRYK
                     ERKLGSEKTNEKKFHFLRRFTQNTYTHYNYHFNAINKLNEVVERAKMAYKDDYTHLLP
                     FYNYTIEATSAAKDDLDSVIYKCTAGILLHDLRNDWIDNLYMILGKAYLYKKDFDSAA
                     GCFQYINYTYAPKDDGYDVPLGSNASKTKGVFTISTKEKNNFWKTITSRPPSRNESFI
                     WQIRNYLEQDLLGEAAGLIGILRNDPNFPKRLKPELHEMTAYWFYKQANYDSASIHLI
                     KALSNADNKAEKARWEFLAGQLFQLANTNDSMAIVMYKKAIKHTNDPLMEVYARLNMV
                     SLASEKKQDALQENLTELLKLTKKERYADYRDIIYYAIAKIELQRNGLDAAQNYFLKS
                     IKYSIKNPQQKTLSFIALGHLNYNRKRFIESHSFYDSVKLDNAKNLDNEEKNLIAKRK
                     PALKVIAENMLAIKKEDSLQRIASMPEAERNIYVKKILKKLRKEKGLKANEEEGAYGA
                     TAVTDTKSTDMFSTDSKGDWYFVNENLKSKGFSEFKAKWGKRANIDNWRRQSSFETST
                     STKTKTRKTTKTTDEIPDTVDDIGSPRETKDDATGTEDTEGKVTEKENEVEDLSFEGL
                     MSKLPLDEERMTASHKKIQGSLFIIGVVFQDHLDEYTEAITNYQTLINKYSNFTERQK
                     TFFNLYYCYTKLGMKVQADSVLGVLKELYPDGNFTQKILKGNTDSTSKIKTDEATKTY
                     ETIYNLFIEGKFKEAIEAKTNADKIYSKNYWTPQLLFIEAIYYVKQKDDSTATVKLKE
                     LIKLYPKTTLAAKAATMIDVLKRRKEIEDYLTKLEVERNEDPVTRNVDTEDDATVVGV
                     KKEKKTNEDTKEAPDKSTTKTEGTKKSTEEKKGVYNFIATDQQYIMIVFNKVDNLFIK
                     ESKDAFAQFNRQKFYSKKINIIQHQLDEENTILLFGIFENTGLALEYYNVLKPQATTR
                     IISFIPPNKYKYSIISQANFNILKDSKNLTEYYNFVQQILPDKF"
     gene            3210..>4055
                     /locus_tag="PLY81_06980"
     CDS             3210..>4055
                     /locus_tag="PLY81_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNF30008.1"
                     /translation="MKKSVKIVWQITFGGIVAFVLFILLCSWGAFGKMPDLEDIQNPS
                     ASLSSQVYAQDGSIMGKYYLQDRINIQFKNIAQPVLDAVIATEDERFYTHSGIDLRAL
                     GRAFAGLGRDGGASTITMQTAKNLFTDNWATKNFIVRSIQKFKEAIIAVKLEKNFTKE
                     EIITLYLNTVAFSDNVYGIRNAAKTFFQKEPDSLNVKESAVLIGMLKGATLYNPRRNY
                     KTSFERRNVVMNQMVKNNKLSKASFDTLKLQPIDLNYRKLDENAGIGPYFRMILGEEL
                     KKWCKE"
BASE COUNT         1702 a          530 c          658 g         1165 t
ORIGIN      
        1 gtacttattt tgtaatcttt ttaatgtatt ttgctgaata ttattttcca tgagtgttaa
       61 attgaaatta aaggtaatga tatagcttaa ttgctaaact tatatttgca aaatacttgt
      121 atttttaact gcttatgttt atggcagaaa attttatgaa gcatcaatta aaaattaata
      181 aaactttttt agcaataata agttgtgtgt atgtttttgc acaaccaaat accgatgtag
      241 atttatacaa agaaaaaccg caacgatata aagaacgcaa attaggctcg gagaaaacaa
      301 acgaaaaaaa atttcatttc ttaagaagat ttacgcaaaa tacttacaca cattataatt
      361 atcatttcaa tgcaattaat aagttaaatg aagtagtaga aagagctaaa atggcttata
      421 aagatgatta tacacatttg ttgccatttt ataattatac aatagaagca acttctgcag
      481 caaaagatga tttagattcg gttatatata aatgcacagc aggtattctt ttacatgatt
      541 taagaaatga ttggatagat aatttgtata tgattttagg caaagcatat ttatacaaaa
      601 aagattttga tagtgctgct ggttgctttc aatatattaa ttacacttat gctcctaaag
      661 atgatggcta tgatgttcct ttaggtagta atgcaagtaa aacaaaaggc gtttttacaa
      721 tttctacaaa agagaaaaat aatttctgga aaacaataac ttctaggcca cctagcagaa
      781 acgaaagttt tatttggcaa ataagaaatt atttagaaca agatttatta ggagaagctg
      841 caggcttaat aggtatctta agaaacgatc ccaactttcc taaaagatta aaaccagaat
      901 tgcatgaaat gacagcatat tggttttata aacaagccaa ctatgatagt gcatctatac
      961 atttaataaa agcccttagt aatgcagata ataaggcaga aaaagcaaga tgggagtttt
     1021 tagctgggca gttatttcaa ctagcaaata ctaacgatag catggctatt gtaatgtaca
     1081 aaaaagccat taaacatact aacgatcctt taatggaagt ttatgccagg cttaatatgg
     1141 taagtcttgc atcagaaaaa aaacaagatg cattacagga aaacttaacg gagttactaa
     1201 aacttaccaa aaaagaaaga tatgccgatt atagagatat tatttattat gcaattgcca
     1261 aaattgaatt acaaagaaat gggttagatg ccgcccaaaa ttattttctt aaaagtatta
     1321 agtatagcat aaaaaatcca caacaaaaaa ccctcagttt tattgcttta ggtcatttaa
     1381 actataatag aaaacgtttt atagagtcac attcatttta cgatagtgta aaattagata
     1441 atgccaaaaa tttagataat gaagagaaaa atttaattgc taaaagaaaa ccagcattaa
     1501 aagtaattgc agaaaatatg cttgccataa aaaaagaaga tagtttacaa agaattgcaa
     1561 gtatgccaga ggcagaaaga aatatttatg taaaaaaaat attgaaaaaa ttaagaaagg
     1621 aaaaaggatt aaaagcaaat gaagaggaag gagcctatgg agcaactgct gttacagata
     1681 caaaaagtac cgacatgttt tctactgatt caaaaggcga ttggtatttt gtgaatgaga
     1741 atctaaaatc aaaaggcttt tcagaattta aagctaagtg gggcaaaaga gcaaatatag
     1801 ataactggag aaggcaatca agttttgaaa catccacttc aacaaaaact aaaacaagaa
     1861 aaacaaccaa aaccaccgat gaaattcccg acactgtaga tgatattggt tcaccaagag
     1921 aaactaaaga tgatgcaacc ggaacagaag acactgaagg aaaagtaaca gaaaaagaaa
     1981 atgaagtgga agatttatct tttgaaggat taatgagcaa gttgccttta gatgaagaaa
     2041 gaatgacagc ttctcataaa aaaattcaag gttcgttatt tattattggt gttgtttttc
     2101 aagatcattt agacgagtat acagaggcta ttaccaacta tcaaacacta ataaataaat
     2161 attccaactt tacagaaagg caaaaaacct tctttaattt atactattgt tataccaaat
     2221 taggtatgaa agttcaagca gattctgttt taggagtatt aaaagaatta taccctgatg
     2281 ggaattttac acaaaaaatt ttaaaaggaa atacggactc tacctcaaaa attaaaacag
     2341 atgaagcaac taaaacatac gaaacaattt ataatttgtt tatagaaggc aagtttaaag
     2401 aagccataga agctaaaaca aatgccgata aaatttacag taaaaattac tggaccccac
     2461 aattattatt tattgaagca atttattatg taaaacaaaa agacgattca acagcaacag
     2521 taaaacttaa agagttaatt aaactatatc ctaaaacaac attggctgca aaagctgcaa
     2581 ccatgattga tgttttaaaa agaagaaaag aaatagaaga ttatttaaca aaattagaag
     2641 tagaacgaaa cgaagaccct gtaacaagaa atgtagatac agaggatgat gcaactgttg
     2701 taggagttaa aaaagagaaa aaaacaaacg aagacaccaa agaagctcca gataaaagta
     2761 ccacaaaaac tgaaggaacg aaaaagtcaa cagaagagaa aaaaggtgtt tataacttta
     2821 ttgcaacaga ccagcaatac attatgattg tatttaataa agttgataat ttattcatta
     2881 aagaatcgaa agatgcattt gcacaattca acaggcaaaa attctattcc aaaaaaataa
     2941 atattataca acaccaacta gacgaagaaa atacaatttt actttttgga atatttgaaa
     3001 atacaggttt ggctttagag tattacaacg ttttaaaacc acaagccact accagaataa
     3061 tttcatttat tccacctaac aaatacaagt atagtattat aagccaagct aattttaata
     3121 ttttaaaaga tagtaaaaac ttgacagagt attataattt tgtacagcaa attttaccag
     3181 ataagtttta aaataagcac taattaaaca tgaagaaatc ggttaaaata gtttggcaaa
     3241 ttacatttgg tggcattgtt gcatttgttc tatttatatt attatgcagt tggggtgctt
     3301 ttggtaaaat gccagattta gaagacattc aaaacccatc agcatcatta agtagccagg
     3361 tatatgcaca agatggttcc ataatgggca aatattattt gcaagatcgt attaatatac
     3421 aatttaaaaa tatagcccag cctgttttag atgcagttat agcaaccgaa gatgaacgct
     3481 tttatacaca tagtggaatt gatttaagag cattaggaag agcctttgca ggcttaggaa
     3541 gagatggtgg tgcaagcacc ataaccatgc aaacagcaaa aaacctattt acagataatt
     3601 gggctactaa gaattttatt gtaagaagta ttcaaaaatt taaagaagca attattgctg
     3661 ttaagctaga aaaaaatttt acaaaagaag aaattattac actctatcta aatactgtag
     3721 catttagcga taatgtttat ggaataagaa atgcagcaaa aacatttttt caaaaagaac
     3781 cagatagttt aaatgttaaa gaatctgcag ttttaattgg aatgcttaaa ggagcaacac
     3841 tttataatcc aagacgtaat tacaaaacat cttttgaacg tagaaatgtt gtaatgaacc
     3901 aaatggtgaa aaataataaa ttaagtaaag caagttttga tacattaaag ctacaaccca
     3961 tagatcttaa ttatagaaaa ttagatgaga atgctggcat aggtccatac tttagaatga
     4021 ttttaggaga ggagttgaaa aaatggtgta aagag
//
LOCUS       DAOQHP010000197         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13403_length_4055_cov_3.465500, whole genome shotgun sequence.
ACCESSION   DAOQHP010000197 DAOQHP010000000
VERSION     DAOQHP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13403_length_4055_cov_3.465500"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLY81_06985"
     CDS             complement(<1..573)
                     /locus_tag="PLY81_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNF30009.1"
                     /translation="MSLTNNTAILLLNDGTIFYGKAFGKIGTTTGELCFNTGMTGYQE
                     VFTDPSYTGQILIMNNVHVGNYGIKENEVESDSIKINGLIGRNLEEQFSRIQANQSMQ
                     EYLAQNNLVAIEGVDTRALVAHVRTKGAMNCIISSDEQDITILINKLANCPNMDGLEL
                     ASTISTNQPYELGNKEAEIKIAVLDFGIKKN"
     gene            complement(672..1184)
                     /gene="rplQ"
                     /locus_tag="PLY81_06990"
     CDS             complement(672..1184)
                     /gene="rplQ"
                     /locus_tag="PLY81_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNF30010.1"
                     /translation="MRHGDKINNLGRKKAHREALLSNLASQLLVHKRIVTTLAKAKAL
                     RTYIEPLITKTKKGDDKNALSHQHRIVFSYLQDKAATKELFTVIAPKVVSRPGGYTRI
                     IKLGIRPGDNAEKAMIELVDFNEIYTKGAVKAAEPAKKTRRSAGAKKKATETTSASET
                     TTETENKTEE"
     gene            complement(1264..2268)
                     /locus_tag="PLY81_06995"
     CDS             complement(1264..2268)
                     /locus_tag="PLY81_06995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNF30011.1"
                     /translation="MAILNFQKPDKIVLQKATDFEGQFEFRPLEPGYGLTVGNALRRV
                     LLSSLEGYAITGIKIDGVDHEFATIKGITEDVTEIILNLKQVRFKKKVEHDVTNEKLT
                     LSIKGNTEFNAGNIGDASLNFEVMNPQLVICTMDNTAKLDIELTINKGRGYIPAEDNK
                     VKDTPTGYIAIDSIHTPIKNVKYSIENYRVEQRTDFEKLILEVATDGTIHPEEAVKQA
                     SRILIQHLMIITDENITFDNKEDKKEDVVDEQVLQLRKVLKTPLEDLDLSVRAFNCLK
                     AAKINSLSELVQYEQEDLMKFRNFGQKSLSEIEQVLTERGLNFGMDLVKLGLDNLDNL
                     "
     gene            complement(2399..3004)
                     /gene="rpsD"
                     /locus_tag="PLY81_07000"
     CDS             complement(2399..3004)
                     /gene="rpsD"
                     /locus_tag="PLY81_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNF30012.1"
                     /translation="MARYTGPKTKISRIFGEPILGNGKWLTKNSNPPGQHGAAKKRKT
                     LGEYALQLREKQKAKYTYGVLERQFRKTFEEASRMKGVKGENLIKLLEARLDNTIYRM
                     GLAASRPEARQLVAHKHITVNGEVMNIPSYSCKAGDVITYRAKSEHNSSITSKSRGKN
                     PKFSWLDWNETEKKGTFITYPERESVPENIKEQLIVELYSK"
     gene            complement(3127..3537)
                     /gene="rpsK"
                     /locus_tag="PLY81_07005"
     CDS             complement(3127..3537)
                     /gene="rpsK"
                     /locus_tag="PLY81_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNF30013.1"
                     /translation="MAKPAKAQNSAKAAAKKRVVKVDAAGEVRINATFNNIIISFTNK
                     SGQVISWSSAGKMGFRGSKKNTPYAAQMAAADAAKVALDAGLKKVDVFVKGPGSGREG
                     AIRSIAQNGIDVTSIKDVTPIPHNGCRPPKKRRV"
     gene            complement(3670..4050)
                     /gene="rpsM"
                     /locus_tag="PLY81_07010"
     CDS             complement(3670..4050)
                     /gene="rpsM"
                     /locus_tag="PLY81_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNF30014.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYVYGIGRSTAQYILTKAGIDFDKK
                     VNQWNDDEQAAIRNIINEEFKVEGALRSETSMNIKRLLDIACYRGLRHRKGLPVRGQR
                     TKTNSRTRKGKRKTVAGKKKAPKK"
BASE COUNT         1181 a          732 c          561 g         1581 t
ORIGIN      
        1 atttttctta atgccaaaat ctaaaactgc aatttttatt tctgcttctt tattacccaa
       61 ctcataaggt tgattagtac ttatggtgct tgctaattct aaaccatcca tattggggca
      121 attagctaat ttatttatta aaattgtaat atcttgttcg tctgaggata taatacaatt
      181 catagcacct ttggttctta cgtgtgccac caatgctctt gtatctaccc cttcaatagc
      241 aaccaaatta ttttgtgcta aatattcttg catagattgg ttggcttgta ttctgctaaa
      301 ttgttcttct aaatttcttc ctattaatcc gtttatttta atactatcgc tttctacttc
      361 attctcttta attccataat taccaacatg aacattattc attattaaga tttgaccggt
      421 ataactaggg tctgtaaata cttcttgata acctgtcata cctgtattaa aacaaagctc
      481 accagttgtt gtaccaattt ttccaaatgc tttaccataa aaaatggtac catcatttaa
      541 aagtaaaatt gctgtattat tagttaagga catgatgttt taaatggttt tgaatattgc
      601 aaaataaaaa aggcaagaag taatttcctt gccttttctt gaaaactttt gaacatttaa
      661 ttaaattaaa attattcttc ggttttattt tcagtttctg tagttgtttc tgaagcacta
      721 gtagtttcag ttgctttctt tttagctcct gcacttctac gagttttctt agcaggttct
      781 gctgctttaa ctgctccttt agtgtaaatt tcattgaaat ctactaattc aatcattgct
      841 ttttctgcat tatcaccagg cctaatgcct aatttaataa ttcttgtata accacctggg
      901 cggctaacaa ctttaggtgc aattacagta aacaattctt ttgtagcagc tttatcttgt
      961 aaataactaa atacaatacg atgttgatgg cttaaagcat ttttatcatc gcctttttta
     1021 gtttttgtaa ttaaaggttc aatataagta cgtaatgctt tagctttagc taatgtagtt
     1081 acaatacgtt tatgaactaa taattgagaa gctaaattgc ttaaaagggc ttctctgtgt
     1141 gcctttttac ggcctaaatt gtttatttta tctccgtgac gcatgacttt tattttgtag
     1201 cttataccgt gtcaggaatt ataagcttat gattaaataa tttaagcaaa tgcttaaact
     1261 gttttataaa ttatctaaat tatctaaacc aagttttact aaatccatac caaaatttaa
     1321 acctctttcg gttaaaactt gttctatttc tgataatgat ttttgaccaa agtttctaaa
     1381 cttcatcaag tcttcttgtt cgtattgtac taactcgcta agagagttaa tttttgctgc
     1441 tttcaaacaa ttaaaggctc ttactgaaag gtctaaatct tctaaaggtg tttttaatac
     1501 tttacgcaat tgtaacactt gttcatctac tacatcttct tttttatctt ctttgttatc
     1561 aaaagtgatg ttttcatcag ttataatcat caagtgttga attaaaatac gagatgcttg
     1621 tttaactgcc tcttcaggat gaatagtacc atcagtagca acttctaaaa ttaatttttc
     1681 aaagtcagtt ctttgttcta cacgataatt ttctatggaa tattttacat ttttaatagg
     1741 tgtatgtatg gagtcaatag caatatatcc ggtaggtgta tcttttactt tattatcttc
     1801 tgcaggtata taaccacggc ctttattaat agttaattca atatctagct ttgcagtatt
     1861 atccatagta caaattacta attgcggatt cattacttca aagtttaaag aagcatctcc
     1921 tatatttcct gcattaaact cggtatttcc ttttatagat aaagtgagtt tttcgttggt
     1981 aacatcatgc tccacttttt tcttaaatct aacttgttta agatttaaaa taatttcagt
     2041 aacgtcttca gtaatacctt taatagtagc aaactcatgg tcaacaccat caattttaat
     2101 acctgtaata gcgtaccctt caaggctgct taaaagtact ctacgtaaag cattaccaac
     2161 ggttaaacca taccctggct ctaacgggcg aaattcaaat tgtccttcaa aatcagtagc
     2221 tttttgtaaa acaattttat ccggcttttg gaagtttaaa atggccattt tatattcctg
     2281 tttttatttg atttaaaaaa aatttgtttc catcttaggt gcaatattca actttagttg
     2341 cgtcgcacac ttgtacagtt gtaagtgtat tcaattatag cagtaatact gttataaatt
     2401 atttagaata caactccact atcaattgtt ctttaatatt ttctggaact gattctcttt
     2461 caggataagt aataaatgtt cctttcttct cagtttcatt ccaatctaac cagctaaact
     2521 tagggttttt accacggctt ttgctagtta tagaactatt gtgttcgctt tttgcacgat
     2581 atgtaataac atctcctgct ttacaagagt aagatggtat gttcattacc tctccattaa
     2641 cagttatgtg cttatgagca actaattgtc ttgcttctgg acggcttgca gctaaaccca
     2701 tacgatagat agtgttgtct aaacgagctt ctaataattt aattaaattt tcgcctttaa
     2761 cacctttcat acgagaagcc tcttcgaaag tttttctaaa ttgacgttct aaaacaccat
     2821 aagtatattt tgctttttgc ttttctctaa gctgcaaagc atattcacct aatgttttac
     2881 gtttttttgc agcaccatgc tgaccgggag ggttactatt tttagttaac catttgccat
     2941 tgcctaaaat tggctctcca aaaattctag aaattttggt cttaggacca gtgtaacgtg
     3001 ccataatttt tatttcattt cgattttttt tgaatcggta aaactatcgg ttaaaaatcg
     3061 taaaaaataa atagtttacc ctttgatata aatgaaacct gaacggctca atataaaatt
     3121 tataaattaa actcttctct ttttaggtgg tctacaacca ttgtgaggta taggtgttac
     3181 gtctttaata gatgttacat caataccatt ttgtgcaata cttctaattg caccttcacg
     3241 tccagaacct ggacctttta caaatacatc tactttcttt aaccctgcat ctaaagccac
     3301 tttagcagca tctgctgcag ccatttgtgc tgcataaggg gtatttttct tagaacctct
     3361 aaaacccatt ttaccagcag atgaccaaga aataacttga ccagatttgt tggtaaatga
     3421 aataataatg ttgttgaaag ttgcattaat acgaacttct ccggcagcat ctactttaac
     3481 tactcttttt tttgctgctg cctttgcact gttttgtgct tttgctggtt ttgccatttt
     3541 taattttttt aggtaatcgt ctgtttctat tttacgtctc ctgttaagca gcttttgccc
     3601 aactatccaa cgtattattt aaaaaaccaa aagctcttaa ctgtttgtta tgagctttat
     3661 agttatttat tactttttag gtgctttctt tttacctgct accgttttac gttttccttt
     3721 tctggtacgg ctattggttt tagtacgctg tccacgaact ggtaatccct ttctgtgtct
     3781 taaaccacga taacaagcaa tatctaataa acgtttaata ttcatagatg tttcgctacg
     3841 caaagcacct tctactttaa actcttcatt aataatatta cgaatagcag cttgttcatc
     3901 atcattccac tgatttactt ttttatcaaa atcaatgcct gctttagtta aaatatactg
     3961 agcagtagaa cgaccaatac catacacata ggttagccct atttcgcctc ttttattttt
     4021 tggtaaatct ataccggcaa tacgagccat aattt
//
LOCUS       DAOQHP010000198         4047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13452_length_4047_cov_4.427605, whole genome shotgun sequence.
ACCESSION   DAOQHP010000198 DAOQHP010000000
VERSION     DAOQHP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13452_length_4047_cov_4.427605"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="nusA"
                     /locus_tag="PLY81_07015"
     CDS             complement(<1..841)
                     /gene="nusA"
                     /locus_tag="PLY81_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNF30015.1"
                     /translation="MTRINLIDAFQDFKDAENLDRPTLMKVVEDVFKTLLRKRYGADD
                     NFDVIVNAEKGDLEIIRRRMIVEDGEVLDPLAEIGYTEATKIDPDFEVGEDLYEEVNM
                     IDFGRRAILAAKQTLASRINDLKKNVLVKKYADRIGEIISAEVYQVWKKEVLLLDEDG
                     NELILPKSEQIPQDFFKKGENIRAVVEKVDLKNNAPVIILSRTSHKFLAKLLEIEVPE
                     IFDGLITIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INYT"
     gene            complement(886..1473)
                     /locus_tag="PLY81_07020"
     CDS             complement(886..1473)
                     /locus_tag="PLY81_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HNF30016.1"
                     /translation="MKKILRVIKIWNINNYYATFATEIETDERNEVVPFFFIPMSADS
                     NLNKIEIFINGLLQSEPNYFLVDLKVKPTNNIKVFIDGDEGITIEKCIYFNRKLYKLI
                     EEAGIYEEGNFSLEISSPGVGEPLKLKRQYQKNIGRNIEVIFIDESKKDGKLLDVMED
                     SILVENTTGKGKKAVTQQLVISFLNIKTTTVQIKF"
     gene            1533..2117
                     /locus_tag="PLY81_07025"
     CDS             1533..2117
                     /locus_tag="PLY81_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNF30017.1"
                     /translation="MIKNICKICVVFVFSFLFITKDLIAQEAAKVPEIDKSPMDISYF
                     PKDYPLMKMNGKSKDLPIARVIYSRPMKKGREIFGGIIQYKEIWRLGANEATEIEFFR
                     NVKIAGKTIAKGRYSLLCIPQPTKWTIIFNKDNYSWGSFNYDAKKDLLRVDCTLEKTE
                     EAIEALSIYFEPLKNGADLIIAWDNTKTKLAITW"
     gene            2220..3269
                     /locus_tag="PLY81_07030"
     CDS             2220..3269
                     /locus_tag="PLY81_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF30018.1"
                     /translation="MFTTKNLSPKQLSAFTSFIVSLPASLVFFVIEKQVWIGFAAFVV
                     MFIGSYFLISFVLEWFIYRKIKLIYKFIFNTKASKKEETYYKYILPKKSIDEVKEDVE
                     KWADQRKAEIELLKQNEAFRKEFLQNISHEFKTPIFAIQGYIEALQAEGLKDNETNQK
                     FLNNAANNVERMVNLVNDLDEITKLERGEQPLNKSNFIIQDLIKEVYESLSIKTNKYN
                     FNTSIKKGCDQPIIVFADKEKIRQVITNIISNATYYGKQNGSIVASIYNTDETNVLVE
                     FSDDGKGISEEHLPRVFERFYRTDKGRSRNDGGSGLGLAICKHIIEAHNQTIHIRSTV
                     DVGTTIGFTLGSAVN"
     gene            complement(3271..>4047)
                     /locus_tag="PLY81_07035"
     CDS             complement(3271..>4047)
                     /locus_tag="PLY81_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNF30019.1"
                     /translation="DLYDQVLPQYGIPKELKYLSVIESNLNPGTISSAGAVGPWQLMS
                     YEGRKYGLRMTEYNDERMDFYKSTHAACKLLNELRNTFNDWLLVIAAYNGGVGRVKQA
                     MKKAKSKNFWDIQYYLPLETRNHVKKYIATHYIFEGSGGWTTMTEQETNQYIASFALE
                     TKQSALTDEEIKNTAIVEVGGRFLSVIIANNLLLDIDQFNKWNPKFDKTLAEGKKYSM
                     RISIDKLPLFEAKKSQILLESMNVLLNDNALVNNRKLQNN"
BASE COUNT         1373 a          621 c          568 g         1485 t
ORIGIN      
        1 atgtataatt aatcacatca atattttcat tctttaattc acgaacaata ccatgtatac
       61 gagaaccttt cataccaaca catgctccaa ccggatcaat tctgtcatca taagattcaa
      121 cagctacttt agctctttcg cccggttcac gaacaatttt cttaatagta attaaaccat
      181 caaaaatttc tggaacctca atttctaaaa gcttagctaa aaatttatga cttgttcttg
      241 aaagaataat tactggagca ttatttttta aatctacttt ttctacaaca gctctaatat
      301 tttctccttt tttgaaaaag tcttgtggta tttgttcaga ttttggtaaa attaattcat
      361 ttccatcttc atctaacaat agtacttctt tcttccaaac ctgataaact tctgcactaa
      421 taatttcccc aattctatcg gcatattttt ttaccaatac gtttttcttt aaatcgttta
      481 tacggcttgc cagggtttgt ttggcagcta aaattgctct tcgtccaaaa tcaatcatat
      541 ttacttcttc gtaaagatct tcgcctactt caaaatctgg gtctattttg gtagcttcag
      601 tatatccaat ttctgctaag gggtctagta cttcaccatc ttccacaatc attcttcttc
      661 gaataatttc taaatcgcct ttttcggcat taactattac atcaaaatta tcatctgcac
      721 catatctttt tcttagaagt gttttaaata catcttctac caccttcatt aaggttggtc
      781 tgtcaagatt ctctgcatct ttaaaatcct gaaaggcatc aattaagttt atccttgtca
      841 tataatttaa atttaaaaaa tgaagcaaaa agttgcttct tctagctaaa atttaatttg
      901 aactgttgtt gtttttatgt ttaaaaaaga aattactaat tgttgggtaa ctgctttctt
      961 cccttttcct gttgtgtttt ctactaagat ggagtcttcc ataacatcta aaagcttacc
     1021 atcttttttg ctttcatcta taaaaattac ttctatattt ctaccaatgt ttttttgata
     1081 ctgtcttttt aattttaatg gttctcctac tcccggactc gaaatttcta aactaaaatt
     1141 accttcttcg taaatacctg cttcttctat tagcttgtat aattttctat taaaataaat
     1201 acatttttca atggttattc cttcatcgcc atcaataaat acttttatat tattggttgg
     1261 ctttaccttt aaatcaacca aaaagtagtt tggctcgctt tgtaataagc cattaataaa
     1321 aatttcaatc ttgtttaaat tagaatctgc tgacataggt ataaaaaaga aaggaacgac
     1381 ctcgttcctt tcatctgttt caatttccgt tgcaaatgta gcataataat tatttatgtt
     1441 ccaaattttt attactctta aaattttctt caaattttct taaaagtatt taactaagta
     1501 tataattata tttttgcata aagttttgta ctatgattaa aaatatatgt aaaatttgtg
     1561 ttgtttttgt atttagtttt ctttttatta caaaagattt aatagctcaa gaagcagcca
     1621 aagttccaga aattgataaa agcccaatgg atattagcta ttttcctaaa gattatccat
     1681 taatgaaaat gaatggtaaa agtaaagatt tacccattgc cagagtaatt tatagcagac
     1741 caatgaagaa agggcgtgaa atatttggcg gaataattca atacaaagaa atatggaggt
     1801 taggagcaaa tgaagctaca gaaattgagt tttttagaaa tgtaaaaatt gctggtaaaa
     1861 ctattgcaaa aggcaggtat agtttattat gtattcctca accaactaaa tggactatta
     1921 tttttaataa agataactac tcttggggca gttttaatta cgatgcaaaa aaagatttac
     1981 ttagggtaga ttgtacacta gaaaaaacag aagaagccat agaagccctt agtatatatt
     2041 ttgaaccatt aaaaaacggt gcagatttaa ttatagcctg ggataatacc aaaacaaaat
     2101 tggcaattac ctggtaaagt tttatgcaag taaaaaggat tgctacttta taaaacttaa
     2161 caatagtttg tttattatta ttattttatc aattaaaaac atagtagttt cgtagcacta
     2221 tgtttactac caaaaacctt agcccaaaac agttatctgc ttttacctct tttatagttt
     2281 ctttaccagc cagcttggtt ttctttgtta ttgaaaagca agtgtggata ggatttgctg
     2341 cttttgtagt gatgtttata ggcagctatt ttttaattag ttttgtttta gaatggttta
     2401 tttatcgtaa aataaaactg atttataaat ttatttttaa tacaaaggct agtaaaaaag
     2461 aagaaaccta ttataaatat attttaccta aaaaaagtat tgatgaagta aaagaagatg
     2521 ttgaaaaatg ggcagatcag agaaaagcag aaattgaact tttaaaacaa aacgaggctt
     2581 ttagaaaaga gtttttgcaa aatatttcgc atgaatttaa aacacccatt tttgccattc
     2641 aaggttacat agaagcttta caggctgagg gtttaaaaga taatgaaaca aaccagaagt
     2701 ttttaaataa tgctgcaaac aatgtagaaa ggatggttaa tttagtaaat gatttagatg
     2761 agattaccaa attagaaaga ggggaacagc cattaaataa atcaaatttt attattcaag
     2821 atctaattaa agaagtgtat gagtcgcttt ctataaaaac caataaatac aatttcaata
     2881 ccagtattaa aaaaggttgc gatcaaccca ttattgtttt tgctgataaa gaaaagattc
     2941 gacaagtaat tacaaatatt attagtaatg ctacttatta tggaaaacaa aatggaagca
     3001 ttgtggcaag tatttacaat acagatgaaa ccaatgtatt agttgaattt agtgatgatg
     3061 gtaaaggaat atctgaagag catttgccta gggtttttga aagattttat agaacagata
     3121 agggtagaag cagaaatgat ggaggatctg gcttagggtt agcaatttgt aaacatatta
     3181 ttgaggcaca caatcaaaca attcatatac gaagcactgt agatgtagga acaacaattg
     3241 gctttacctt aggttctgca gttaattaaa ctaattattt tgtaattttc tattatttac
     3301 taaagcgtta tcatttaata atacattcat actctctaaa agtatttggc tttttttagc
     3361 ttcaaataaa ggtaatttgt caatagaaat tctcattgaa tattttttac cttctgctaa
     3421 agttttatca aattttgggt tccatttatt aaactgatct atatctagca ataagttgtt
     3481 tgcaataata accgataaaa atctacctcc aacttcaaca atagcagtat tttttatttc
     3541 ttcatcagta agagcagatt gcttagtttc taaagcaaat gaggctatat attgatttgt
     3601 ttcttgttca gtcattgttg tccatccacc agagccttca aaaatataat gagtagcaat
     3661 atatttttta acatggtttc tggtttctaa tggtaaataa tattgaatgt cccaaaagtt
     3721 tttgctttta gcttttttca tggcttgttt cactctgcca acaccaccat tgtatgctgc
     3781 aataactaat aaccagtcat taaacgtatt gcgaagttcg tttaatagtt tacaagctgc
     3841 atgtgtgctt ttgtaaaaat ccattctttc atcattgtac tctgtcattc ttaatccata
     3901 ttttcttcct tcgtagctca ttaattgcca cgggccaaca gcaccagctg aagaaattgt
     3961 acctggattt aaattacttt ctattacact taagtacttt aactcttttg gaataccata
     4021 ttgcggtaaa acttgatcgt ataaatc
//
LOCUS       DAOQHP010000199         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13871_length_3972_cov_4.449834, whole genome shotgun sequence.
ACCESSION   DAOQHP010000199 DAOQHP010000000
VERSION     DAOQHP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_3972_cov_4.449834"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /gene="wecB"
                     /locus_tag="PLY81_07040"
     CDS             complement(<1..751)
                     /gene="wecB"
                     /locus_tag="PLY81_07040"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNF30020.1"
                     /translation="MKNILLVIGTRPEAIKIAPLIIKLQQQKALFNTKVCLTAQHRQM
                     LDQVLEFFEIKADYDLDLMKPNQNLHSLTSDIITAIKPVYDEFKPDWVLVHGDTTTST
                     AAALAAFYSGAKVAHVEAGLRTFNKLAPFPEEMNRSITGRIADLHFAPTKASKQNLLN
                     EAVSKETISITGNTVIDALKLGITKVNEQSSEIANLKSNINLNKKIILVTGHRRENFG
                     DGFENICKALKIIAENNFDDVEIIYPVHLNPN"
     gene            complement(779..1819)
                     /locus_tag="PLY81_07045"
     CDS             complement(779..1819)
                     /locus_tag="PLY81_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30021.1"
                     /translation="MEIEEKEISIKDLLKSFKSWFWYILKQWWKIAGIAIIGAVIAIA
                     YRLWIPINYTAKSSFVVEEGRTSTGSLASLAGQFGFDIAGSSGATLFTGDNILIFFKS
                     NSLTKETLLTEYDSITKFSLADKYAEVYGLREKWAESKRIGKEIFFKTNQTTSLSRLQ
                     DSLLQTIVVKVLKKEFSVERPEKKATFIVVQCSFRDEMLSKLFCERIVKKATEWYVES
                     KTRRHRINVERLQKRADSIEQVLNGRTFYTAATQETTLDVNPAASTAAVKLEVSNRNK
                     LMVTQVYAEVIKNLEIGKVQLSQETPTILEVDNPDFPLPKTKRQFILYSLIGMIIFSF
                     FSIIYFTIKRLV"
     gene            complement(1841..>3972)
                     /locus_tag="PLY81_07050"
     CDS             complement(1841..>3972)
                     /locus_tag="PLY81_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNF30022.1"
                     /translation="TSSAAIHESRTSVTTQKPDHSSKPTTIFGSELFSNPSLSFEPDL
                     RLPTPKNYTLGPDDKLLLDVFGVNLSQQNLTVGPEGTVYVKYAGPIQVNGLTIEDASK
                     IITKKLSKFYPAISNGQTKVVLSLTNIRAIKVMVLGAVRKPGTYNISSLATLFNALYV
                     SGGPTENGSFRNIELVRNNKVLLVADLYDFLINSNQKNNIRLMDNDIIKIPYAKVQVS
                     IAGEVNREGIYELQPKENLNKLIEYAGGYQSNAFKARITGLRNTDFDRQVLDIIKDSI
                     NTFIPQNGDSYNVGSIINKFQNRLIIEGAVFKPGNYALTTGLTVQELLHKAEGLRGDA
                     YTGRAVLVRTKDDLTKEIISINLLSDSGKNFILQKEDVLQVTSIFDIKEKFEVTINGA
                     VRKPGAYSFEDSLSLKTLILRAGGFAYNALGTDIVIARRKRNLDINNPKSSIVELIKI
                     NDDKDLSNPTSDIQLKPFDIITIKEDPYYKSQISVAISGQVLSPGSYTLTSRVEKISD
                     LINRAGGTLYTANIDGARLLRKNSVREDELALIDKIAEASAHDSSGVTANSQNKLYKV
                     IAIDLIKIMSNPGDKEDIFLEEGDIIEVPLKNNMVTIAGEVFNPIVVGYGENKKVRDY
                     LNDAGGIAKSGNKRKVFIVYPNGSAARTKKILGIFRKYPTVTPGSKIFVPREPEKKAT
                     DYAKAGIIVTGLSALITAVALAFQITK"
BASE COUNT         1201 a          679 c          543 g         1549 t
ORIGIN      
        1 catttggatt taagtgaacc ggataaataa tttcaacatc atcaaaatta ttttcagcaa
       61 taatttttaa tgctttacaa atgttttcaa atccatctcc aaaattttct cttctatgcc
      121 cagttactaa aattattttt ttatttagat taatatttga ttttagattt gctatttcgc
      181 tgctttgctc attaactttt gtaataccta atttaagtgc atcaataact gtattacctg
      241 taatagaaat agtttcttta gaaactgcct cgtttaataa attttgttta gaagcttttg
      301 tgggggcaaa atgcaaatct gcaattctgc ctgtaatgct tctattcatt tcttctggga
      361 aaggtgctag tttattaaat gttcttaatc cagcttcaac atgagccact tttgccccag
      421 aataaaaagc tgctaatgca gcagcagtag atgttgtagt atcgccatgt actaaaaccc
      481 aatctggttt aaattcatcg tacacaggtt ttattgcagt aataatatcg gaagttaagg
      541 aatgtagatt ttgatttggt ttcattaaat ctaaatcata atctgctttt atttcaaaaa
      601 attcaagtac ttgatccaac atttgcctat gttgtgcagt taagcaaact ttagtattaa
      661 ataatgcttt ttgttgttga agtttaataa ttaatggggc tatttttatt gcttccggcc
      721 ttgtaccaat taccaataat atgtttttca taaacctgtt atgctataaa taaaataatt
      781 aaaccaatct ttttatggtg aaataaataa ttgaaaagaa tgaaaatata atcatgccaa
      841 ttaaagagta tagaataaac tgcctcttag ttttaggtaa aggaaaatct ggattatcca
      901 cttctaaaat agttggagtt tcttgactta gttgcacttt accaatttct aagtttttaa
      961 taacctctgc atatacttgt gttaccatta atttatttct attactaacc tccagtttaa
     1021 cagctgctgt agatgcagca gggtttacat ctaaagttgt ttcttgagta gcggctgtat
     1081 aaaaagttct tccatttaaa acctgctcaa tactatcggc tcttttttgt aatctttcaa
     1141 catttattct atgtcttctt gttttagatt ctacatacca ctctgtagct ttttttacta
     1201 ttctttcaca aaagagttta cttaacattt catctctaaa gctacattgc actacaataa
     1261 aggttgcttt tttctctggt ctctctactg aaaattcttt ctttaaaacc tttactacaa
     1321 ttgtttgtaa caaactatct tgtagtcttg ataggcttgt tgtttggttg gttttaaaaa
     1381 atatttcttt acctattctt ttactttctg cccacttttc cctaagccca tacacttcgg
     1441 catatttatc tgctaatgaa aatttggtta tagagtcata ctcggtaagt aaagtttctt
     1501 ttgttaaact attgctttta aaaaatatta aaatattatc tcctgtaaat aaagtagcac
     1561 cagacgaacc tgcaatatca aatccaaatt gcccagctaa agaagccaga cttcctgttg
     1621 aagttctacc ttcttctacc acaaaggaac tttttgcagt ataatttatg ggtatccata
     1681 atctatatgc aatagcaatt acagcaccaa taattgcaat accagcaatt ttccaccatt
     1741 gttttagtat gtaccaaaac caggatttaa aagattttaa aagatctttt attgaaattt
     1801 ctttttcttc tatttccatt agttgcaaat attaaaatgt ttattttgta atttgaaaag
     1861 ctaatgctac agcagtaatt aatgcagata atcctgtaac tattattcca gcttttgcat
     1921 agtcggttgc tttcttctct ggctctcttg gaacaaatat tttacttcct ggtgttactg
     1981 taggatattt tctaaaaatg cccaatattt tttttgttct tgctgcacta ccattagggt
     2041 aaacaataaa tacttttctt ttattaccag attttgcaat gcctcctgca tcgtttaaat
     2101 aatcccttac ttttttattt tctccatacc caactacaat tgggttaaat acttcgcctg
     2161 caatggttac catattattt tttaatggta cttcaataat atctccttct tctaaaaaaa
     2221 tatcttcttt atcaccaggg tttgacatta tctttattaa atcaattgca attactttat
     2281 atagtttgtt ttgtgagttt gcagtaacac cagatgagtc gtgtgctgat gcttctgcaa
     2341 ttttatcaat taaagcaagc tcatcttctc ttacactatt ttttcttaaa agtcttgctc
     2401 catcaatatt tgcagtatat aaagtacccc ctgccctatt tattaaatcg ctaatttttt
     2461 ctactctact tgttaatgta taagaaccag ggcttaatac ttggccagaa atggcaacag
     2521 aaatttgcga tttataataa ggatcttctt tgatagtgat aatatcaaat ggttttaatt
     2581 gtatatcact agtagggttg gataagtctt tatcgtcatt aattttaatt aattcaacaa
     2641 tagagctttt ggggttatta atgtctaaat ttcttttcct tctagcaatt acaatatcag
     2701 tgccaagggc attataagca aaaccacctg ctcttaaaat taaggttttt aaggataggc
     2761 tatcttcaaa agaatatgct ccaggcttac gaacagctcc attaatagta acttcaaact
     2821 tttctttaat atcaaaaata gaagttactt gtaatacatc ttctttttgt aaaataaaat
     2881 ttttaccaga atctgataat aaattgatgc ttataatttc tttggttaaa tcatcttttg
     2941 ttcttactaa tactgctctt cctgtatagg catctcccct taacccttct gccttatgca
     3001 ataattcttg tacagttaaa cctgttgtta gtgcataatt tccaggctta aaaacagctc
     3061 cttctattat taacctgttt tgaaacttat tgattatact tccaacatta tacgaatcgc
     3121 cattttgagg aatgaaggta ttgatagagt cttttataat atctaacact tgcctatcaa
     3181 aatctgtgtt tcttaacccg gttattcttg ctttaaaagc gttgctttga tagcctcctg
     3241 catattctat taacttgttt aaattttctt taggttgtag ttcataaatt ccttctctat
     3301 ttacctcgcc agcaatactt acttgcactt ttgcataggg aattttaata atatcgttat
     3361 ccattaacct gatattattt ttttgattgc tgttgattaa aaaatcgtat aaatcagcca
     3421 ctaataatac tttgttattt cttaccaatt caatatttct aaatgagccg ttttcagttg
     3481 ggccaccact aacgtataaa gcattaaata aagttgctaa tgaactaata ttataggttc
     3541 caggttttct tacagcaccc aaaaccataa ctttaattgc cctaatattg gttaaggaca
     3601 atactacttt tgtttgtcca ttagatattg caggataaaa tttagataat tttttagtga
     3661 ttattttaga cgcatcttca atggttaagc cattaacctg aattggtcct gcatatttta
     3721 catatactgt tccttctggt cctacagtta aattttgttg agaaaggttt acgccaaata
     3781 catctaataa taatttatca tcggggccta atgtatagtt tttgggagta ggcaatctta
     3841 aatctggttc aaaacttaaa cttggattag agaaaagttc ggagccgaaa attgtagttg
     3901 gctttgaact atggtctggc ttttgagtag ttacacttgt tctgctttca tgtattgcag
     3961 cactggatgt at
//
LOCUS       DAOQHP010000200         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13906_length_3967_cov_2.956033, whole genome shotgun sequence.
ACCESSION   DAOQHP010000200 DAOQHP010000000
VERSION     DAOQHP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13906_length_3967_cov_2.956033"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLY81_07055"
     CDS             <1..533
                     /locus_tag="PLY81_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30023.1"
                     /translation="LYYENLEMQVNALPHKNFREYKNKPTFYTYYFAGGAHAIKRKIL
                     NQTGNLPDAFFYGMEEYDLSYRIIQFGYCIKYDASIIMLHKESPLGRKPKQEKLQMMW
                     VNKSIVAYRYLPIGYFYTTAIMWSLQYLRETNFNIKGFFIGWKNVLKIFKQEKRTPLN
                     HQALTYLNKTQARLWY"
     gene            complement(594..1154)
                     /locus_tag="PLY81_07060"
     CDS             complement(594..1154)
                     /locus_tag="PLY81_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF30024.1"
                     /translation="MAQGKELPEVIETKDIFVGDKKAPVTLMEFGDYESEDCAKVNEV
                     VKKILEEFSGVVKFNFRHFPLTKIHQRSQKAAEAAISAAQDGKFWEMHNALFANRRNL
                     GTTTLKLHAKDAGINRKEMLEELMNGTYGQNVHEDIAYGKKIGVTEIPTFFVNGVMFS
                     GKPSFENLSKEIIAASKKVKPAKKSK"
     gene            complement(1271..3733)
                     /gene="clpB"
                     /locus_tag="PLY81_07065"
                     /pseudo
     CDS             complement(1271..3733)
                     /gene="clpB"
                     /locus_tag="PLY81_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
BASE COUNT         1192 a          741 c          487 g         1547 t
ORIGIN      
        1 ttttatatta tgaaaactta gaaatgcaag taaatgcatt acctcataaa aattttagag
       61 aatataaaaa caagccgaca ttttatacct attattttgc tggtggtgca catgccatta
      121 aaagaaaaat attaaaccaa actggtaatt taccagatgc ttttttttat ggtatggaag
      181 agtatgattt gagttacaga attatacagt ttggatattg tattaagtac gatgcatcta
      241 taattatgct gcataaagaa agccctttag gtagaaagcc caaacaggag aaattacaaa
      301 tgatgtgggt aaacaaatct attgttgcct atagatattt accaattgga tatttttata
      361 ctactgcaat tatgtggagt ttgcaatatt taagagaaac taattttaat ataaaaggtt
      421 tttttattgg ctggaaaaat gttcttaaaa tttttaaaca agaaaaaaga actccactaa
      481 accatcaggc tttaacctat ttaaataaaa cgcaagcaag gttatggtat taaaaaaccg
      541 tttaaacaac atgtgtaaaa acggctttgc ttactaatac cctattgtat tttttattta
      601 gacttttttg ctggctttac ttttttagaa gctgcaataa tttctttact taaattttca
      661 aatgatggtt taccagaaaa cataacgcca ttaacaaaaa atgtaggaat ttctgtaaca
      721 ccaatttttt taccatacgc aatatcttcg tgtacatttt gcccataagt accattcatc
      781 aactcctcta acatttcttt tctatttata ccagcatctt ttgcatgtag ctttaaagtt
      841 gtagtaccta agtttcttct attggcaaaa agagcattat gcatttccca aaacttacca
      901 tcttgtgctg cagatatggc tgcttcggca gctttttggc ttctttgatg tattttggtt
      961 aatggaaaat ggcgaaaatt aaattttaca acaccactaa attcttctaa tatttttttc
     1021 actacctcgt ttactttggc acaatcttca ctttcataat caccaaactc cattaaggtt
     1081 actggtgctt ttttatctcc aacaaatatg tctttcgttt ctataacttc tggtagttct
     1141 ttgccttgag ccataaaaaa tataggtttt agtttttagt gaggctaaaa aaaagcccct
     1201 tcacaacatt aattgttgta ttggggcaaa catagtgttt tttaatattt agaaatacta
     1261 attctattat ttaattttca gtttcatttc taaataccac aattccgtca aaaacatcca
     1321 ccaaaacggg gttttcctta tttatgctcc cagataatat ttttttactc aattcattta
     1381 ttaccacttt ttgaattaac ctttttaaag gtctggctcc aaattgtggg tcataaccgt
     1441 tttctaccaa ataatcaatt gcataattac taaaatttaa agtaattcca gattgggcaa
     1501 ccattttctt taaactattt aattgaatgt ctataatatt ctttatttgg ctttttaata
     1561 aaggttgaaa cataattatc tcatctattc tatttaaaaa ctctggccta atggtttgct
     1621 ttaataaact catcacttct acttttgcct tttctattat ttcttcaata ttagtttcat
     1681 ttaattctac aaatgcttct tgaataagat ggctaccaat attactagtc attataatta
     1741 tggtattttt aaaattcacc attcttcctt tattatctgt aagccttcca tcatctaaaa
     1801 cctgtaataa cacattccac acatcgggat gggctttctc aatttcatct aaaagaacaa
     1861 cgctgtatgg ctttcttcta acagcttctg ttaattgtcc tccttcgtcg taacctacat
     1921 atcctggagg tgcaccaacc aaacggctta cagtatgttt ttcctgatat tcactcatgt
     1981 ctattcttgt catcatactc tcatcatcaa ataaatactc agctaaagct tttgctaact
     2041 ctgttttacc tacacctgtt gtacccaaaa aaataaaact accaattggt tttttagggt
     2101 cttgtaagcc tgccctactt cttctaattg catcagaaac agcagtaatg gcttcatctt
     2161 gtcctaccac acgtttgtgt aactcatctt ctaagtttag taatttctct ctttcgcttt
     2221 gtagcatttt acttacaggt atgccagtag ctttcgcaat agcttctgca atatcttctg
     2281 catctacttc ttcttttaaa agccttcttt cgcttatagc atctaagctt ttgctaaatg
     2341 tttctatcgt tttttcctgt tctttaattt taccatatct tatttctgca actttcccaa
     2401 agtcgccgtc tctctctgct ctttcagctt ctgtttttaa actttcaata ctggctttag
     2461 cattttgaat tttttctaca atttcttttt cttctttcca cttagctttt aaggtatccc
     2521 tttgtacagt tattaaagaa atttcctgag aaagttcttt taacttcaat tcatcatttt
     2581 ctctttttat tgcttctttc tcaatttcta attgcctgat ttgcctttct agaatatcta
     2641 attcctcagg cattgaattc atttctagtc taagttttgc agcactttca tcaattaaat
     2701 caattgcttt atctggtaaa aatctatctg taatgtaacg atgagataac tctactgcag
     2761 caataatggc ttcatcttta attctaacat ggtggtgtgt ttcatactta tcttttatac
     2821 ctcttaaaat agaaactgca tcttccacat ttggttcatc aataaatact ttttgaaaac
     2881 ggcgttctaa ggctttatct ttttcaaaat atttttgata ttcattcatt gtggttgcac
     2941 caattgctct cagctctcca cgtgctaatg ctggctttaa aatattggct gcatccatgg
     3001 caccttctcc accacctgca ccaattaaag tgtgtatctc atcaataaat aaaataagtt
     3061 cgccattgct ttcagtaact tcttttacaa cactttttaa tctttcttca aattcgcctt
     3121 tatactttgc acctgctata agtaaaccca tatctaaggc aaaaattatt ttacttttta
     3181 aattttctgg aacatctcca ttaacaattc tcatagctaa cccttcggca attgctgttt
     3241 tacctacacc aggctctcca actaaaattg gattattttt tgacctccga gttaaaatat
     3301 gtaatgttct tcttatttct tcatctctac caattacagg atctaactta ccattgcttg
     3361 ctaaatcgtt taaattttta gagtattttt gtaacgcatt atactgttgg ttttgtgttt
     3421 gagagtttac tgtactgcct ttgcgtaaat cttttattgc tgctattagc cccttttctg
     3481 ttaagcctgc atcttttaat gttttactca cctcatcatt tacactaagc aaaccaatta
     3541 acaaatgttc tacacttaca aattcatcgt taaattgttt taacgaggat actgctttta
     3601 gtaaagcatt gttcaaatcc ctgcctatat tttctggtgt ttgaccattt gaatttttgg
     3661 gtaacttctt tatgctttca tctaattttt tttcaacaaa aacaacgttt acatcattct
     3721 tttttaataa aaaataacta aatcagttca attatcagac tattaaagcc agctgatcct
     3781 gaaattcaaa aaatattaaa agttaacaaa aataatcttt cattcttgaa aaaaattaat
     3841 gtaatttttt aatgaagaaa tcaaaacaat acattttcga taacgattta tatttaatat
     3901 gaaattaatc aaacaaatca ttggattgcg atcataagta ggcgtgtttg gctttaacgt
     3961 tgactgt
//
LOCUS       DAOQHP010000201         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13908_length_3966_cov_4.353618, whole genome shotgun sequence.
ACCESSION   DAOQHP010000201 DAOQHP010000000
VERSION     DAOQHP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13908_length_3966_cov_4.353618"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLY81_07070"
     CDS             complement(<1..639)
                     /locus_tag="PLY81_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30025.1"
                     /translation="MLFTLLKKIIKTATGRTKFIMAFIGLSVALLLILSAIQIQANYN
                     QLLYGKNNTDSTANFLVVNKEVNDNTVNATILSNEVLHEIKQQPFIDAVGYISGGRYK
                     ASIESLIQSFPFYTDIAFESVQNDFIDVNSKDWAWNENSNFIPMIIPNMFLDIYNFQF
                     SISQGYPQITPETLKMMRFRVNLYYANGNSENYIGRIVGFSDRISSVLVPQSF"
     gene            complement(645..1382)
                     /locus_tag="PLY81_07075"
     CDS             complement(645..1382)
                     /locus_tag="PLY81_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF30026.1"
                     /translation="MASIKIKQNTNFYIHFLIRYLVLSVPIKHSMQIRLNKIIPIPLK
                     DKLLQRKSDVWNTDLIFNIQQLVKINAPSGTGKTTLIHILYKLRNDYSGIVKWNDELL
                     ENINDDKLANYRKHKLSIVFQDLKLFQNLTAKENIELNRVLQKPYYEEKVIVEMAEEL
                     GVDSILQQPIRLCSYGEQQRIAIIRALIQPFELLLMDEPFSHLDYDNTVKAAALIAKE
                     CTKRNAGFVLTDLDDDNHFLYSQKLRL"
     gene            1408..>3966
                     /gene="mutS"
                     /locus_tag="PLY81_07080"
     CDS             1408..>3966
                     /gene="mutS"
                     /locus_tag="PLY81_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF30027.1"
                     /translation="MAKAASVSPMMQQHKAIKQKYPDAILLFRVGDFYETFGQDAINA
                     SQVLGITLTKRNNGDVGNTELAGFPHHALDTYLHKLVKAGYRVAICDQLEDPKQAKGV
                     VKRGVTELVTPGVATNDKLLENSNNNFLAAIHFAESNVGIAFLDISTGEFFVAEGNEE
                     YIDKLLQSLKPAEVIFQRSSQKQFKETFGVKFYTYTLESWIFELGFATESLIKHFQTH
                     SLKGFGIENMQHSIIAAGAILYYLKDTEHSNLQHITTIQRIEREDFLWMDKFTIKNLE
                     IIGSPGAEGNNLLKVLDNTVSPMGARLLKRWVVMPLKNLDKINERLQVVEDFIVDTDN
                     RLQLINAIKHCGDIERLVSKIPLKKINPREVIQLAKGLQQVAIIKKICSSFKNPYLQR
                     LSDNLNNCTVILEKILTEIQEEPPVTTNKGGIIKTGVHHELDELRKIASGGKEYLINI
                     QQREADTTGIPSLKIGFNNVFGYFLEVTNTHKNKVPASWIRKQTLANAERYITTELKE
                     YEEKIIGAEEKIITIELEIFEKLLLALQDYIAPIQVNGNIVAIVDCLLCFSENALKYK
                     YKKPQLHNGLELNLKESRHPVLERNLPEGKAFIANDIYADADSQQIIILTGPNMSGKS
                     AILRQTALITLMAHIGSFVPVDTAKIPLTDKIFTRVGASDNLSGGESTFMVEMNETAS
                     IINNISNRSLILLDEIGRGTSTYDGISIAWSIVEYLHQSTAKPKTLFATHYHELNELE
                     EKLTRVKNYHITNKEVGNKIIFLRKLAKGGSTHSFGIHVAKMAGMPQELINRANEILK
                     ILEQKTEIPEEQNIKPMIQDLGDKIKSLNTPKLQLSIFDTQTQVFEDIRKTLEGI"
BASE COUNT         1473 a          582 c          624 g         1287 t
ORIGIN      
        1 aaaactctgt ggtaccaata cactcgaaat tctatcacta aaaccaacaa ttctgccaat
       61 gtaattttct gagtttccgt ttgcataata aagattaacc ctaaaacgca tcatctttaa
      121 ggtttctgga gtaatttgag gatagccttg agaaatggag aattgaaaat tataaatatc
      181 taaaaacatg ttgggaataa tcataggaat aaaattagag ttctcattcc aagcccaatc
      241 tttactattc acatcaataa aatcattttg tacactttca aatgcaatat cagtataaaa
      301 aggaaaagat tgaattaaac tttctataga tgctttatat cttccaccac ttatatatcc
      361 aactgcatca ataaaaggct gctgttttat ttcatgtaaa acctcattac ttaaaattgt
      421 tgcatttact gtgttatcat ttacctcttt gtttactact aaaaaattgg ctgtactatc
      481 tgtattattt ttgccataca ataattgatt atagtttgcc tgtatttgta tggcagataa
      541 aataagtaac aatgcaaccg ataggccaat aaaagccatt ataaactttg ttctgccggt
      601 agctgtttta attatttttt ttaaaagtgt aaaaagcatt ttaattaaag ccttaatttt
      661 tgtgaatata aaaaatgatt gtcatcatca aggtctgtta atacaaatcc tgcatttctt
      721 ttagtgcatt cttttgcaat taatgcagct gctttaacag tattatcata atctaaatgg
      781 ctaaatggct catccattaa caacaactca aagggttgaa ttaaagctct tattatagca
      841 attcgttgtt gctctccata gctacaaagc cttatgggtt gctgtaaaat agaatcaaca
      901 cctaattctt cagccatttc tacaattacc ttttcttcat aatatggttt ttgtaaaacc
      961 ctgttaagct caatattttc ttttgcagta agattttgaa aaagttttaa atcttgaaaa
     1021 acaatgctta atttatgttt acgataatta gccaatttat cgtcattaat attttccagc
     1081 aattcatcat tccattttac aataccagaa taatcatttc taagtttata caatatatgt
     1141 attagtgtag ttttacctgt gccactgggt gcatttattt ttaccaactg ttggatatta
     1201 aaaattaaat cagtattcca aacatcagat tttctctgta gtaatttatc tttaagtggt
     1261 ataggtatta tcttattaag ccttatttgc atactatgtt taataggtac cgaaagtact
     1321 aaatatcgta ttaagaaatg gatataaaaa ttagtatttt gtttaatttt tattgatgcc
     1381 aatgtacaat gtttatattt gctaactatg gcaaaagctg cttctgtatc gcctatgatg
     1441 caacaacata aagcaattaa acaaaaatac cccgatgcta ttttgttgtt tagagttggt
     1501 gatttttacg aaacttttgg gcaagatgca atcaatgcat cgcaggtgct gggcattaca
     1561 cttactaaaa gaaataatgg tgatgtaggt aatacagaat tggcaggctt tcctcatcat
     1621 gcactagata cttacctaca taaattagta aaagctggtt atcgtgttgc tatttgcgat
     1681 cagttagaag accctaaaca agccaaaggt gttgtaaaac gtggtgttac agagttggtt
     1741 acacctggcg ttgctactaa cgataaactt ctggaaaata gtaataataa ttttttagca
     1801 gctatccatt ttgctgaaag taatgtaggt attgcctttt tagatattag tactggagag
     1861 ttttttgtag cagaaggaaa tgaagaatat atagacaaac ttttacaaag cttaaagcct
     1921 gcagaagtta tttttcaaag aagctcacaa aaacaattta aagaaacatt tggcgtaaaa
     1981 ttttatacct acactttaga aagttggatt tttgaacttg gctttgcaac agaaagttta
     2041 ataaaacatt ttcaaacaca ttctttaaaa gggtttggaa ttgaaaatat gcagcactct
     2101 attattgctg ctggtgccat tttgtattat ttaaaagata cagaacattc aaaccttcaa
     2161 catataacaa caatacaaag aatagagaga gaagattttt tgtggatgga taaatttaca
     2221 atcaaaaatc ttgaaattat tggcagccct ggtgcagaag gtaataactt attaaaagtt
     2281 ttagacaata ctgtttcgcc aatgggtgca aggctactta aacgctgggt agtaatgcct
     2341 ttaaaaaatt tggataaaat aaatgaacgg ttacaagttg tagaagattt tatagtagat
     2401 acagataatc ggcttcaact aattaacgca ataaagcatt gtggcgatat tgaaaggttg
     2461 gttagtaaaa ttcctttaaa aaaaataaat ccaagagagg ttatacaact tgctaaagga
     2521 ttacaacaag ttgccattat taaaaaaatt tgttcctcct tcaaaaaccc ttacttacaa
     2581 aggctttcag ataatttaaa caattgtacg gtaattctgg aaaaaatatt aaccgaaatt
     2641 caggaagagc ctcctgttac aactaataaa ggtggaataa ttaaaactgg tgttcatcat
     2701 gagttagatg agctaagaaa aatagcttcg ggtggtaaag aatatttaat taatattcaa
     2761 caaagagagg cagatacaac aggtattcct tctttaaaaa ttggatttaa taatgttttt
     2821 ggatattttt tagaagttac caatacacat aaaaataaag tgcctgcaag ctggataaga
     2881 aaacaaactt tagcaaatgc agaaagatat attacaacag aacttaaaga atatgaagaa
     2941 aaaataatag gtgctgaaga aaaaattatt acaattgaac ttgaaatttt tgaaaaatta
     3001 ttacttgcat tacaagatta tatagcaccc atacaagtaa atggtaacat agtagctatt
     3061 gtagattgtt tactttgctt ttctgaaaat gccttaaagt acaaatacaa aaagcctcaa
     3121 ctgcataatg ggcttgaatt aaatttgaaa gaaagtaggc acccagttct tgaaagaaat
     3181 ttaccagaag gcaaagcatt tattgcaaat gatatttatg cagatgcaga tagccaacaa
     3241 ataatcattc ttactggccc aaatatgagt ggtaaaagtg ccatattaag gcaaacagca
     3301 ctaataacat taatggctca cattggcagc tttgtaccag tagatactgc aaaaattcct
     3361 ttaacagata aaatttttac ccgtgttggg gcaagtgata atttaagtgg cggagaaagt
     3421 acttttatgg tagaaatgaa tgaaaccgcc tccatcatca ataatatttc taacagaagt
     3481 ttaattttat tagacgaaat tggcagggga acctctactt acgatggtat tagcattgct
     3541 tggagtattg tagaatattt acaccaatct accgcaaaac ctaaaacatt atttgcaaca
     3601 cattatcacg aattaaatga attggaagaa aaattaaccc gtgttaaaaa ttatcatatt
     3661 accaataaag aagttggaaa taaaattatt tttttacgaa aattagccaa aggtggcagt
     3721 acacatagtt ttggtattca tgtggcaaaa atggcaggaa tgccacagga gttaataaac
     3781 agagcaaatg aaattttaaa aatattagaa caaaaaacag aaataccaga ggagcaaaat
     3841 ataaaaccaa tgatacaaga tctaggagat aagataaaat ctttaaacac tcctaaatta
     3901 caactctcta tttttgatac acaaacacag gtttttgaag atattagaaa aacattagaa
     3961 ggtata
//
LOCUS       DAOQHP010000202         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13965_length_3954_cov_3.754296, whole genome shotgun sequence.
ACCESSION   DAOQHP010000202 DAOQHP010000000
VERSION     DAOQHP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13965_length_3954_cov_3.754296"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3077)
                     /locus_tag="PLY81_07085"
     CDS             complement(<1..3077)
                     /locus_tag="PLY81_07085"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30028.1"
                     /translation="MKKFLSLLFMLFFFMITSNAQEKDQTDIREMYVRIAVVKKINND
                     SVIALIDGGKRYGIKNGSVGIVKGVYKSGDDRSDFEIGYASVYKVTDTTAFVTIKPAD
                     KLGTKKGYDIRKGDYVILKVNVPKLTYRSIFFELALLDIQFNNLYNNSLYSIADLFYR
                     DSKQLEDSLLIAGSKDVFETYDALKNDTSFKSLNEPLTEGRYKGRSIFGVMKDCNAND
                     VFSFLHFVSNYPGKYIGHTWKLNETFATWVLNKAPYSKKEIYDSIMSYKNKPALLKVF
                     ITKNIALIKQKGFVTDWISDAATALNLKQTQKALNFFEAAKLVLPYLKDESITGFYYF
                     NYAGYFEDLEKYNQVINYYDTAYSYYTKCNNDYFKTRILLSKGLTYSSMNKLDDAIAT
                     FDKMYADLSKADNQLTNSQKNIFYAQYHYYSGFVQAKKGELKKAVSHYEEAIKLYKSF
                     NTYSSLNLAINVQGRLARLYKQQGELQKSLQIYTEQLELYTNLNDRKNIADVLDNIAD
                     IEFKLANYRKAIQQYSTAKDIHLSFEDYNSAGFSQSNIGQAFWNLGKYDSAIAAHQIA
                     LQYRSISKSYSGLGYTWKKIGALYKETGEKTKALSAYDSSAHYYSLGKDSSNLRSLLQ
                     DIGDVYYNDKQYQKSFQYYFKWHQLNIATKNKSDIVNSSYWLAWAAYDLKLIDTSKYY
                     FNYCLNTAKDIGDKANQMYANLGLGSVAYKEYAYEEGEKYFNTALEIAIAEKNKKQEA
                     NGYKIKANAYATKLDFANALIYFNKSIALYDSLGEKTALPALYNNVANAYQIKGDFEE
                     SKKWYQKSIDNARKTNNKSEIGDAFSGLSFLYVLQGNLDLAQTAADSSEAVFKELNNN
                     WQIAGSYITMGIVNNYKNNYNASVKYYLTADSLFTLEKDDWSRSTCLNNIGNVYYFQA
                     DYNNALKYFLESEKLLSKIKIVTESHILAKNNIGETYYYLKNYTKAEKYLLEGYALAK
                     EKNIGRMLSSANIELGMLYFDTKKYAESEKYLMDGQTIAKKMNLLENIVASNM"
     gene            complement(3329..>3954)
                     /locus_tag="PLY81_07090"
     CDS             complement(3329..>3954)
                     /locus_tag="PLY81_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNF30029.1"
                     /translation="FTLTFAVQKKIIKMKTVTIEGQLRTEFGKKATRQIRSQEQVPAV
                     IYGGAQEVNFAASLKSFKPLVYTPEFQLAEIKVGEKTYKCILKDLQFDKVTDELIHID
                     LLELVDDKKVIANLPLKYTGNSIGVKDGGKLVLKMKSVKVKTYPKYLKENLEVDITNL
                     NINGNLRVEDIKADNIEIMNSPRIPIASVVMTRQLKQEEATKDEKKK"
BASE COUNT         1254 a          624 c          524 g         1552 t
ORIGIN      
        1 tacatatttg atgcaacaat attttcgaga agattcattt ttttagcaat ggtttgtcca
       61 tccattaagt acttttcact ttcggcatat ttttttgtat caaagtataa catacctaat
      121 tcaatattgg cgctacttag catacgccca atattttttt cttttgctaa agcataacct
      181 tctaataaat atttttctgc cttagtataa ttttttaaat aataataggt ttcgccaata
      241 ttatttttag caagaatatg gctttcggta actattttaa tttttgataa taatttttcc
      301 gactctaaaa aatattttaa agcattgtta taatctgcct gaaaataata aacattacca
      361 atgttattta aacatgtact acggctccaa tcatcctttt ctaaggtaaa taagctgtct
      421 gcagttaaat aatattttac cgatgcgttg taattatttt tataattatt tacaataccc
      481 attgttatat aactaccagc aatttgccaa ttgttattta gttctttaaa taccgcttcg
      541 ctactatctg ctgcagtttg tgcaaggtct aaattacctt gcaatacata tagaaaactt
      601 aagccagaaa atgcgtcgcc aatttcgctt ttgttgtttg tctttcttgc attatcaata
      661 gatttttggt accatttttt actctcttca aagtctcctt ttatttgata agcatttgct
      721 acattattat ataaggcagg cagtgcagtt ttttctccta atgaatcgta caatgcaata
      781 gatttattaa agtaaataag agcattagca aaatctagtt tagttgcgta ggcattggct
      841 ttaattttat aaccatttgc ttcttgtttt ttattttttt ctgcaattgc aatttctaat
      901 gcggtattaa aatatttctc tccttcttca taagcatatt ctttataggc aacagagcct
      961 agccctaagt tggcatacat ttggttggct ttatcaccaa tatcttttgc tgtattcaag
     1021 caataattaa aatagtactt agaggtgtca attaatttta aatcataggc agcccaagct
     1081 aaccaataag agctgtttac aatatccgat ttgttttttg tagcaatatt taattgatgc
     1141 catttaaaat aatactgaaa agatttttgg tattgtttat cgttataata tacatctcca
     1201 atatcttgta ataaactcct taaattgcta ctatcttttc ctaatgaata ataatgtgca
     1261 ctgctatcat aggcggataa tgcttttgtt ttctctcctg tttctttgta tagagctcca
     1321 atttttttcc atgtataacc taagccagaa taactttttg aaatggagcg atattgtaaa
     1381 gcaatttgat gagcagctat tgcagaatcg tatttaccca aattccaaaa tgcctgacca
     1441 atattagatt gagagaaacc ggcactatta taatcttcaa aagaaagatg aatatctttt
     1501 gctgttgaat actgttgaat tgcttttcgg taattggcca atttaaattc aatatcagca
     1561 atattgtcca atacatcagc aatgtttttt ctatcgttta ggttggtata taattctaac
     1621 tgctctgtat aaatttgtaa agatttttgc aactcacctt gttgtttata taaccttgcc
     1681 aacctgcctt gtacattaat tgcaaggttt aatgagctat atgtattaaa ggatttatat
     1741 aattttatag cttcttcgta atgagaaaca gcttttttta attcgccttt ttttgcttgt
     1801 acaaaaccag aataataatg gtattgagca taaaatatat ttttttgaga attggttaat
     1861 tgattatcgg ccttacttaa atcagcatac attttatcaa acgttgcaat agcatcatcc
     1921 agtttattca tagagctata ggtgagccct ttggataaaa gtattcttgt tttaaagtaa
     1981 tcgttgttgc atttagtata gtaggagtag gcagtatcat aataattaat aacttggttg
     2041 tacttttcta aatcctcaaa atagcctgca taattaaaat aatagaaccc agtaatagat
     2101 tcatctttta aataaggtaa aactaatttt gcagcctcaa aaaaattcaa agctttttgt
     2161 gtttgtttta aatttaaagc agtagcagca tcacttatcc aatctgttac aaaccctttt
     2221 tgttttataa gagcaatgtt tttagttata aatactttta aaagtgctgg tttgttttta
     2281 taactcatta tactatcata aatctctttt ttgctataag gtgctttgtt taaaacccat
     2341 gtagcaaagg tttcatttag tttccatgtg tgaccaatat attttcctgg gtaattactt
     2401 acaaaatgca aaaaactaaa tacatcattt gcattacagt ctttcattac accaaaaata
     2461 cttcttcctt tatacctacc ttctgttaaa ggttcattta ggctcttaaa actagtatca
     2521 ttttttaagg catcataggt ttcaaataca tctttagaac cggctattaa taagctatct
     2581 tctagttgtt tactatctct atagaaaaga tcagcaatgc tgtataaact attgttgtat
     2641 aaattattaa actgaatatc taataaagct agttcaaaaa aaatgcttct gtaggttagt
     2701 tttggtacat taacttttaa aattacataa tcgccttttc ttatatcata ccccttttta
     2761 gtgccaagtt tatcagctgg tttaatagtt acaaaggcag tagtatctgt tactttatat
     2821 acactagcat aaccaatttc aaaatcgctt ctatcgtccc cagatttgta cactcctttc
     2881 actattccta cacttccatt tttaatacca tatcttttgc caccatcaat taaagctatt
     2941 acactatcat tatttatttt ttttacaact gcaatgcgta catacatttc tctaatatct
     3001 gtttgatctt tttcttgtgc attgcttgtt atcatgaaaa aaaacaacat gaaaagaaga
     3061 gagaggaact ttttcatata ttagttattt tgaattaaga aggatgagtg tatagtatta
     3121 aaatataata ggtatagtta atttattttc tgatatactg tttcaatatt attttgatat
     3181 ttaaagttat ataatttaaa acaattgctt gtaccgcttt ttgggtattt tatttatgat
     3241 gataaagaat aactttaaac taacagagta aataaaataa aaaacccttg aaataatcca
     3301 agggtttgaa aaattattta aaatataatt attttttctt ttcatcttta gttgcttcct
     3361 cttgttttaa ctgacgagtc attacaacag aagcaatagg aatacgtgga gagttcataa
     3421 tttcaatatt atctgcttta atatcttcca cacgtaagtt gccattaatg ttaagattgg
     3481 taatatctac ttctaaattt tctttcaaat atttagggta agtttttacc ttaaccgatt
     3541 tcatttttaa tactaattta cctccatctt ttacaccaat tgaattacca gtatatttta
     3601 atggtaaatt ggcaatcact tttttgtcat ctacaagttc taacaaatca atatgtatta
     3661 attcatcggt aaccttatca aactgtaaat cttttaaaat acatttgtaa gttttttcac
     3721 caactttaat ttcggctaac tgaaattcag gtgtataaac caatggttta aaagatttta
     3781 aagaagcagc aaagtttact tcttgtgcac ccccgtaaat tacagcaggc acttgctcct
     3841 gagagcgtat ttggcgggta gcttttttgc caaattcggt cctcagttgt ccttcaattg
     3901 taactgtttt cattttaatg attttttttt ggacggcaaa ggtaagggta aact
//
LOCUS       DAOQHP010000203         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_13973_length_3952_cov_3.342571, whole genome shotgun sequence.
ACCESSION   DAOQHP010000203 DAOQHP010000000
VERSION     DAOQHP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13973_length_3952_cov_3.342571"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLY81_07095"
     CDS             complement(<1..833)
                     /locus_tag="PLY81_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNF30030.1"
                     /translation="MRNVILLIGFLFYVGNASAQTVPSPEEFLGYKIGTKYTRHHQVI
                     DYVRAVAIAKKDMVIAEQYGTTNEGRPLMLLYISTPENIQNLENIRKNNLRITGILKD
                     NVKPQIKNSPSIIWLSYNVHGNEPSSSEAAMLTLYALVNPANTITKEWLKNTVVIIDP
                     CLNPDGRDRYVNWFNQMVGSNEDADPQSREHSEPWPNGRSNHYNFDLNRDWAWQTQVE
                     SQQRIKKYNQWMPQIHVDFHEQYYNNPYYFAPAAEPLHEAITPWQKEFQEIIGRNHAK
                     Y"
     gene            complement(855..1787)
                     /locus_tag="PLY81_07100"
     CDS             complement(855..1787)
                     /locus_tag="PLY81_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNF30031.1"
                     /translation="MIKNWMVTVLLVIVGNLFAQDGSIKSLKEETAKKDIKKNATDTA
                     KSNWKKGGLFSLNLAQGSLSNWAAGGDKFSMSVNLYLNAHAYYKKGKNSWDNNVDFYL
                     GYVNTTTLGSRKNDDRFDLTSKYGYALNPKLNLSSLFNFRTQFYKGYEYSNNNKVLTS
                     NFMAPGYILISLGLDYKPTNDLSIFFSPITSRWVVVNDKNLSALGLYGVKPGKKSVNE
                     IGAFASVNYTKNLNKIISYKGKLDMFSNYRNKPQNIDIFMTNLFSAKISKILSATWSL
                     DLIYDDDVKLFGNNKNSPGLQLKSLFGVGLMVKL"
     gene            1934..3889
                     /locus_tag="PLY81_07105"
     CDS             1934..3889
                     /locus_tag="PLY81_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF30032.1"
                     /translation="MLIGLQNVTFEFGARVIVEDATWHIHPGERIGLIGYNGTGKSTL
                     LKVLTQQYTPSKGTVEKSKDTTIGYLHQDLLSFDTNESIVEVAMSAFEQVKLLEKEIE
                     NLGNQLAENSEDESLLMLYSEKLHDLEVAGGYTVHHKTEEVLQGLGFDNKDLQRPYKE
                     FSGGWRMRVLLAKMILQQPDVLLLDEPTNHLDLPSIEWLEKYLLHYKGSVVIVSHDKY
                     FLNRMVNKIVELYQQQLHIYTGNYDYYETEKAIRVEMQNKAYENQQDYIRQNERLIER
                     FRAKASKASMAQSLMKKLDKLERIETAEIERPNIKINFQVDKQPGKIIASLNNVCKNF
                     GENVIIKNGNAEIDRGDKIALIGANGKGKSTLLRIIAGTEKFDGQREWGHNVEESFYA
                     QHQLEALNLNNTILDEMKECGSQKTELELRALLGCFLFSGDETDKKIKVLSGGEKARV
                     ALAKVIVSKSNFLMLDEPTNHLDMHSVELLAEALNKYEGSLILVSHDRYFISKTANKI
                     WEIIDHEIKEFKGTYTEWVEWNERMAKQKAQSEKQKTAIENDVVIEKKKEEKKTSNTI
                     EQTALKNELKQLQKKISKLEMDINNVNSRKEAVEADLGNPTFYTNHIEFTKLESNYKK
                     IIEEKKKLDKEYEEIFEKMMDVESAIG"
BASE COUNT         1464 a          560 c          629 g         1299 t
ORIGIN      
        1 aaatactttg catgatttct tcctattatt tcctgaaact ctttttgcca aggtgttata
       61 gcttcgtgta atggctctgc tgctggagca aaataataag gattattgta atattgttca
      121 tgaaaatcta catgtatttg tggcatccat tggttatatt tttttattct ttgttggctt
      181 tctacttgtg tttgccaagc ccaatctctg tttaaatcaa aattgtaatg attgcttctg
      241 ccattaggcc aaggttcact atgttctcta ctttgtgggt ctgcatcttc attactacct
      301 accatttgat taaaccaatt tacatatcta tctcttccat ctgggtttaa acaagggtct
      361 ataattacta cagtattttt taaccactct ttagttattg tatttgcagg gtttaccaaa
      421 gcatacaaag ttagcattgc tgcttcgctg ctacttggtt cgttgccatg cacattataa
      481 ctaagccaaa taatactggg cgaatttttt atttggggtt ttacattatc ttttaaaatt
      541 cctgttattc ttaaattatt cttacgaata ttttctaggt tttgaatatt ttctggagta
      601 gaaatatata acagcatcaa gggccttcct tcgttggttg ttccatattg ttcggcaatt
      661 accatatctt tttttgctat ggctactgct ctaacgtaat ctataacttg atgatgcctg
      721 gtgtatttag ttcctatttt gtagcccaaa aattcttcgg gagaaggaac tgtttgtgca
      781 cttgcattac caacataaaa aagaaagcca attaataaaa ttacatttct cataatattt
      841 ttatttttat ctaattataa tttaaccatt aagcccacac caaataaaga ttttaattgc
      901 aatccaggag agtttttatt gtttccaaaa agcttaacat catcatcata aattaaatct
      961 aagctccaag ttgcggataa tattttagaa attttggcag aaaataaatt ggtcataaat
     1021 atatcaatat tctgtggttt atttctataa ttactaaaca tatctaactt acccttgtaa
     1081 gaaattattt tattcaaatt tttggtataa ttaacacttg caaaagcacc aatttcgtta
     1141 acagattttt ttcctggctt tactccatac aatcctaatg cagataagtt tttatcgttt
     1201 acaacaaccc atcttgaagt tataggagaa aagaagatgg ataaatcatt agttggctta
     1261 taatctaaac ctaaactaat taaaatatat ccaggagcca taaaatttga agtaagcact
     1321 ttattattat tactatactc gtagccttta taaaattgag ttctaaaatt aaataatgaa
     1381 cttaaattta atttagggtt aagtgcataa ccatattttg aggttaaatc aaatctgtca
     1441 tcattttttc tactacccaa agtagtagta tttacataac ccaaataaaa atctacatta
     1501 ttatcccaac tgttttttcc ttttttataa tatgcatgag catttaaata taagtttacc
     1561 gacatggaaa acttatcgcc accagcagcc caattactta aagatccttg agctaaattc
     1621 aagctaaata atccaccttt tttccaattg ctttttgctg tatcggtagc atttttttta
     1681 atatcttttt tggctgtttc ctcttttaaa cttttaatgc ttccatcttg tgcaaataaa
     1741 ttacccacta ttactagtaa aacagtaacc atccagtttt taatcataat tattaacttt
     1801 ttagactaca ataataacaa gttaattgct tactcattta ccaattaaac aatttttgaa
     1861 ttatatttta tgttttatta aacctataaa taacagcagc ccattacctt tgctgctttc
     1921 taaaaaataa attatgctga taggtttaca aaatgtaaca tttgaatttg gtgcaagagt
     1981 aattgtagaa gatgctacat ggcacataca cccaggcgaa agaattggct taattgggta
     2041 taatggcaca ggaaaatcta ctttattaaa agtattaact caacaataca caccaagtaa
     2101 aggcactgtt gaaaaaagta aagacaccac cattggttat ttgcatcaag atttgttaag
     2161 ttttgatacc aatgaatcta ttgtagaagt ggcaatgagt gcttttgaac aagttaaatt
     2221 gttagaaaaa gaaattgaaa atttaggtaa ccaattagcc gaaaatagtg aagatgaaag
     2281 cttactaatg ctatacagcg aaaaactaca tgatttagaa gttgcaggcg gttatacagt
     2341 acaccataaa actgaagaag ttttacaagg gttaggattt gataataaag atttacaacg
     2401 cccttataaa gagtttagtg gaggttggcg tatgagggtt ttattagcta aaatgatttt
     2461 acaacaaccc gatgttttat tgttagatga acctaccaac cacttagatt taccaagtat
     2521 tgaatggctt gaaaaatatt tattgcatta taaaggaagt gtagtaattg ttagccacga
     2581 caaatatttc ttaaaccgaa tggtaaacaa aattgttgaa ttgtatcagc aacagttaca
     2641 tatttatact ggcaactacg attattacga aacagaaaaa gctatacgtg tagaaatgca
     2701 aaataaagct tatgaaaatc aacaagatta tataagacaa aatgaaagat taattgaacg
     2761 gtttagagct aaagccagca aggcttctat ggctcaaagc ttaatgaaaa aattagataa
     2821 gttagaaaga atagaaactg ctgaaataga acgtcctaac ataaaaatta attttcaggt
     2881 agataaacaa ccaggaaaaa taattgcctc attaaataat gtatgtaaaa actttggtga
     2941 aaatgtgata ataaaaaatg gaaatgcaga aattgacaga ggagataaaa ttgctttaat
     3001 tggtgcaaac ggtaaaggaa aatctacttt acttagaata attgcaggaa cagaaaagtt
     3061 tgatggacaa agagaatggg gacataatgt tgaagaaagt ttttatgctc agcaccagtt
     3121 agaggcatta aatttaaaca acactatttt agatgagatg aaggagtgtg gaagccaaaa
     3181 aacagaatta gaattaagag ccttattagg gtgtttttta tttagtggag atgaaacgga
     3241 taaaaaaata aaagtattaa gtggtggcga aaaagcaaga gttgctttgg ctaaagttat
     3301 tgtgagtaaa agcaattttt taatgttaga tgaacctacc aaccacttag atatgcatag
     3361 tgtagagctt ttggcagaag ctttaaataa atacgaaggt agtttaattt tagtgagcca
     3421 tgataggtat tttatttcta aaactgcaaa taaaatatgg gaaataattg atcatgaaat
     3481 taaagagttt aaaggcactt acaccgaatg ggtagaatgg aatgaaagaa tggctaaaca
     3541 aaaagcacaa agtgaaaaac aaaaaacagc tatagaaaat gatgttgtta ttgaaaagaa
     3601 aaaggaagaa aaaaaaacta gtaatacaat agaacaaaca gcattaaaaa acgaactaaa
     3661 gcaacttcaa aaaaaaatta gtaagctgga aatggatata aataatgtaa atagcagaaa
     3721 agaagcagta gaggctgatt tagggaatcc aacattttac accaaccata tagaatttac
     3781 taaattagaa agcaactata aaaagattat agaagaaaaa aagaaactag ataaagaata
     3841 cgaagaaatt tttgaaaaaa tgatggatgt agagagtgct ataggatagc tttatttggt
     3901 actatcttat aaactgtgta agtcttcata aagttggggt accccaactt ta
//
LOCUS       DAOQHP010000204         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14104_length_3928_cov_4.342112, whole genome shotgun sequence.
ACCESSION   DAOQHP010000204 DAOQHP010000000
VERSION     DAOQHP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14104_length_3928_cov_4.342112"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLY81_07110"
     CDS             complement(<1..699)
                     /locus_tag="PLY81_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNF30033.1"
                     /translation="MQKSLINFLENTPTFYFVRFDLNGKLTYVNQYYQKHFEQFFIHF
                     LGANFTDSIHPDSIEDFNKSRQQCIDKANCIIKVELRDNKIININTPNYIKWNFFSII
                     NESGEVVEFGGFGVSTSSVRKAKEEHKVTQQKLVALLNSTVESFYYLDKNMNVLAFNS
                     GAKYKTMLYYNIELQEGFYFPKLLVPGTEENFYKQFSNALKGVENYIEDKISYSNGKS
                     IWYKMSCKPVYDDKN"
     gene            883..2160
                     /gene="serS"
                     /locus_tag="PLY81_07115"
     CDS             883..2160
                     /gene="serS"
                     /locus_tag="PLY81_07115"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNF30034.1"
                     /translation="MLQVNYIRQEKEKVKERLQVRNFSNVEIVDSIIALDDTRKNIQL
                     NFDNTQAKINATSKEIGKLMAQGNKDEAEQIKQNVAQWKTEVTNLNTNLINIEKKLQD
                     ALIQLPNLPHESVPKGKTAEDNVTVKEAGKKPVLPANALPHWDLIKQYNLVDFETGAK
                     ITGSGFPLYKGKGAKLQRALVQYFLDYNTSAGYTEFLPPFMVNETSAYSTGQLPDKEG
                     QMYHATEDNFYLIPTAEVPVTNIYRDVILKESDLPIKMTAYSPCFRREAGSFGKEVRG
                     LNRVHQFEKVEIIQIVEPEKSYSVLDEMVAHVEALLTNLELPYRILRLCGGDMGFTSA
                     LTYDFEVWSAAQQRWLEVSSVSNFENFQTNRMKCRYKNAVGKTVLAHSLNGSSLALPR
                     IYASLLENNQTDAGIRLPKALHNYFGDEIITKN"
     gene            2163..2816
                     /locus_tag="PLY81_07120"
     CDS             2163..2816
                     /locus_tag="PLY81_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNF30035.1"
                     /translation="MLVNTETYKKITEELINKATLVAVSKVKPVEAILELYHLGQRDF
                     GENYVQELVDKQSKLPTNINWHFIGHLQSNKVKFIAPFVYLIHGVDSIKLLKEVNKQA
                     AKNNKIIKCLLQVHIAQETTKFGFGLDELNDLFFLSECKNVEVIGLMGMASFSNDLNL
                     VRKEFIQLQQLFKTLQKSNPQFTTLSMGMSDDYKIALEEGSNMIRIGSLLFGKREYN"
     gene            complement(2817..3896)
                     /locus_tag="PLY81_07125"
     CDS             complement(2817..3896)
                     /locus_tag="PLY81_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNF30036.1"
                     /translation="MFQIFQYKQLFHFTEQVFLKIGCSANDATIATKVLLNADIRGID
                     SHGVARLVGYVRLWQAKRINANPAISIIHQTPSTATVDGDKGLGLVVAPYAMQLAINK
                     AKECGTGWVAVQNSNHFGIAGYHAMMALEHDMIGIAMTNASALVAPTFSIEKMLGTNP
                     IAVAIPANKQPAFVADFATTTAANGKLEILQRKNLDTPLGWVQDKDGYPSTDAHIIKN
                     DGALLPLGSDKEHGSHKGYALGSIVDIFSAVLSGASYGPWAPPFPAYIAMPQNMPGKG
                     LGHFFGAMRIDAFRSATDFKQHMDNWIERFRNAKPANGYEKVLIPGDPEREMQEERMN
                     NGIPVIEAVVKDLHNLAQTLDIALL"
BASE COUNT         1398 a          592 c          647 g         1291 t
ORIGIN      
        1 attcttatcg tcgtacacag gtttacagct cattttatac caaatagatt ttccattaga
       61 ataagaaatc ttatcctcaa tataattttc aacacctttt aatgcatttg aaaattgttt
      121 gtaaaaattt tcttcggtac caggtactaa tagtttagga aaataaaaac cttcttgtaa
      181 ttcaatatta taatacaaca tggttttata ctttgcacca ctattaaaag caagtacatt
      241 catgttctta tctaaatagt aaaaagattc tactgtgctg tttaatagtg caactaattt
      301 ttgttgtgta actttatgct cttctttagc ttttctaaca ctagatgtag aaaccccaaa
      361 tccaccaaat tcaactactt caccagattc attaataata gaaaaaaaat tccattttat
      421 ataattaggg gtgtttatat ttattatttt attatcccta agctctactt taattataca
      481 gttagcttta tctatacatt gttgcctact tttattaaaa tcttcaatac tatctgggtg
      541 aatggagtca gtaaaatttg caccaaggaa atgtataaaa aattgttcaa aatgtttttg
      601 atagtattga tttacataag ttagttttcc atttaaatca aatcttacaa aatagaatgt
      661 tggagtattc tctaaaaaat ttattaagga tttttgcatt tcaataaatc tttctttggt
      721 tattaggttt taaaggttct caaacttaat attctatttt taaaaaacaa attttacttt
      781 ttaaatggtt aactatttat atgaaacaac taaaaacatc agcaatagct gaattatttc
      841 aagtaaaata aaattttgta cgtttgcggc aaattaaatt ttatgcttca ggtaaattat
      901 ataaggcagg agaaagaaaa agtaaaagag agattgcagg taagaaattt ttcgaacgta
      961 gaaattgtag atagtattat tgctttagat gatacaagaa aaaacattca gttaaatttt
     1021 gataataccc aagcaaaaat taatgctacc agtaaagaaa ttggtaaatt aatggcacaa
     1081 ggaaacaaag atgaagcaga acaaattaaa caaaatgtag cacaatggaa aacagaggta
     1141 acaaacttaa acactaacct tattaatatt gaaaaaaaac tgcaagatgc tttaatacaa
     1201 ttacccaatt tacctcatga atctgtacct aaaggaaaaa cagcagagga taatgtaaca
     1261 gtaaaagaag ctggtaaaaa acctgtattg cctgcaaatg ctttgccaca ttgggattta
     1321 ataaaacaat ataatttagt tgattttgaa acaggagcaa aaattacagg aagtggtttt
     1381 cctttataca aaggcaaagg tgcaaagttg caaagagctt tagtgcagta ctttttagac
     1441 tataatacta gtgcaggtta cactgaattt ttacccccat ttatggttaa cgaaactagt
     1501 gcatacagta ctgggcaatt accagataag gaagggcaaa tgtatcatgc aactgaagat
     1561 aatttttatt taatacccac tgccgaagtg cctgtaacca atatttacag agatgttatt
     1621 ttaaaagaaa gtgatttacc cattaaaatg actgcctact caccttgttt tagaagagag
     1681 gctggtagtt ttggaaaaga agtgaggggc ttgaataggg tgcaccaatt tgaaaaagta
     1741 gaaattattc agattgttga accagaaaaa agttattctg ttttagatga aatggttgca
     1801 catgtagaag ccttgttaac taacctggaa ttgccataca gaattttacg tttatgtggt
     1861 ggcgatatgg gctttacatc tgctcttaca tacgactttg aagtgtggag tgcagcacaa
     1921 caacgttggc ttgaggtaag tagtgtaagc aattttgaaa attttcaaac taaccgtatg
     1981 aaatgcaggt acaaaaatgc agttggcaaa actgtacttg ctcatagttt aaatggtagt
     2041 tctttggcat tgccaagaat atatgcatct ctattagaaa acaatcaaac agatgctgga
     2101 ataagattac ctaaagcatt gcataattat tttggagatg aaataattac taaaaactaa
     2161 agatgcttgt taatacagaa acatataaaa aaattacaga ggaattaatt aacaaagcaa
     2221 ctcttgtagc tgtaagtaag gtaaagcctg tggaagccat attagaatta taccatttgg
     2281 ggcaaagaga ttttggcgaa aattatgtac aagaattagt tgacaaacaa agtaaattgc
     2341 ctaccaatat taattggcat tttatagggc atttgcaaag taataaagta aaatttattg
     2401 ctccttttgt ttacttaata catggtgtag atagtattaa actattaaaa gaagttaata
     2461 aacaagctgc aaaaaataat aaaattatca agtgtttatt acaggtacac atagcacaag
     2521 aaaccacaaa atttggattt ggattagatg aattaaatga tttatttttt ttaagcgaat
     2581 gtaaaaatgt tgaagtaatt ggcttgatgg gtatggcatc ttttagcaat gatttgaatt
     2641 tagtaagaaa agaatttata caactacaac aactttttaa aacattacaa aaaagtaacc
     2701 cacagtttac taccttatca atgggtatga gcgatgatta taaaattgct ttggaagagg
     2761 gtagcaatat gataagaata ggaagtttgc tttttggtaa aagagagtat aattagttat
     2821 aataaagcta tatcaagcgt ttgtgctaaa ttatgtaaat ctttaaccac agcttcaatt
     2881 accggtattc cattgttcat tctttcttct tgcatttctc tttctgggtc gccgggtatt
     2941 aacacttttt cgtatccatt ggctggtttt gcatttctaa atcgttcaat ccagttatcc
     3001 atgtgctgtt taaaatctgt tgcactacga aatgcatcaa ttctcattgc tccaaaaaaa
     3061 tgacccaagc ctttaccagg catgttttgt ggcatagcaa tatatgcagg aaaaggaggt
     3121 gcccaagggc catagcttgc accacttaat actgcagaaa aaatatctac aatacttcct
     3181 aaagcatagc ctttatggct gccatgttct ttatcgctac ctagtggtaa taaagctcca
     3241 tcatttttta tgatatgagc atctgtagag gggtaaccat ctttatcttg aacccaacct
     3301 aaaggtgtat ctaagttttt tctttgcaat atttcaagct taccattggc agcagtggtt
     3361 gttgcaaaat ctgctacaaa agcaggttgt ttattggcag gtatggcaac agcaattggg
     3421 ttggtgccca acattttttc tatagaaaaa gtgggtgcta ccaatgcact ggcattggtc
     3481 attgcaatac caatcatatc atgctctaaa gccatcatag catgataacc tgcaattcca
     3541 aaatgattac tattttgtac agcaacccaa cctgtaccac attcttttgc tttattaatg
     3601 gctaattgca ttgcataggg agccactaca aggcctaacc ctttatctcc atctactgtt
     3661 gcagtagatg gggtttggtg tattattgaa atggcagggt tggcattaat tcttttagcc
     3721 tgccaaagtc ttacataacc tacaagcctt gctacgccat ggctgtcaat tcctctaata
     3781 tcagcattta ataatacttt tgttgcaatg gttgcatcat ttgctgagca gccaattttt
     3841 aaaaaaacct gttcggtaaa atgaaataat tgtttatact gaaatatttg aaacataata
     3901 tttaaaacaa aaattataaa aataaatt
//
LOCUS       DAOQHP010000205         3923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14133_length_3923_cov_4.175026, whole genome shotgun sequence.
ACCESSION   DAOQHP010000205 DAOQHP010000000
VERSION     DAOQHP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14133_length_3923_cov_4.175026"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2038
                     /locus_tag="PLY81_07130"
     CDS             <1..2038
                     /locus_tag="PLY81_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNF30037.1"
                     /translation="TIDDLLHFVKAPDFPTGGIIYGMEGVKQGLLTGRGRVVLRGKCH
                     VETKQSGREQIIITEVPYQVGLDALTDKIGQLVNDKTIEGIAHVNNESNKREGIRIVI
                     DLKRDAVANIIINQLYKFTELQTSFGINNVAISKGRPRIFNVKDLIVEFVDFRMDVVI
                     RRSKFELKKAEDRAHLLEGYLKVINDKDHLDLAISIIRGSATPDEAKKSLIERSIADN
                     WYLPDNLFDEKAKIHYLQGIGLSEKQAQAILELRLQRLTGLEREKIIEEFNDLMNTIV
                     RLKDLLASEDLRYDLIKTELEEVKAKFGEPRKSEIQFLADEVNMLDFIKEEDVVITVS
                     HLGYIKRTSATEYRQQKRGGRGAIGSKTREEDFVEHLFVASTHDVMMFFTEKGRCYWL
                     NVYEIPEGEKQSKGRAIQNLIQIPPDDKIKAIIDVKDLDKKETLQNRYVVLCTKKGII
                     KKTDLEDFSRPRTTGINAITILEGDELLEAKLTDGNCEIMMAVKSGRAIRFNEEKVRA
                     TGRGAIGVTGIEVDETNDEVVGMICVNKNDTDKTVLVISEKGYGKRTKLTDEDGEDVY
                     RITNRGGKGVKTINVTEKTGRLVGILDVNEKEDLIITCQSGITLRTSISTIKTAGRNT
                     QGVILIRLDAEDAIAAISKIEETEEETTTEVNDQSENNSPENTDNLNTNIENSN"
     gene            2049..3446
                     /locus_tag="PLY81_07135"
     CDS             2049..3446
                     /locus_tag="PLY81_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30038.1"
                     /translation="MKKIILLVACSISLVIANAQDKMYKKLQTLYIAAQYEEAKAEIE
                     KVMANPKAIDKAETWLWRTRIYAEIYFSEPLRKLNPGCGNIAFESFKKYEALESNYKM
                     LANPELSAWRWRPLDLIYVTSFNLGRSFFEEKKWDSSYNTFMKSVYMGDVIVKNNLRE
                     NGAKIDTVSVLYAAYAAQNGKMEAEATALYEKFADCKIGGKDFKDAYMYILLYASKTK
                     DKAKFNKYIAIAKEVYPNDDAWDDYEMEFITNTTTVEERIAYYDKEDAAGTLTARKYM
                     LFGQSFSEATKGEEAEKLDSLTKVRYDKKAVEAFKKAYQKDNKLGIAAYNAGLLYYNE
                     FVEYDDRISNNRRALQQLNSNKPVVKDPKKKAVADAEFKKQTDAIKKLNSDLEKPILE
                     TLDSCVVWLEIAYNNLKNGNMEDRTTLNCIKNSVKWLANAFMYKREKVKGKDTKAYDA
                     YDAKYNEYDKLYEKY"
     gene            3553..>3923
                     /locus_tag="PLY81_07140"
     CDS             3553..>3923
                     /locus_tag="PLY81_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF30039.1"
                     /translation="MQLGTVYLIPTVLFEDELHCIPDYVVDTIKNCEIFFTENEKTTR
                     RFFKKLWKEMVIDNYQWHTIHKTEQAVVTTFLQHLKDGKNVGIVSEAGCPGIADPGQI
                     LVEAAQQINAIIKPLVGPSSI"
BASE COUNT         1621 a          478 c          729 g         1095 t
ORIGIN      
        1 tacaattgat gatttattgc attttgttaa agcacccgac tttccaacag gtggcattat
       61 ttatggaatg gaaggcgtga agcaagggct attaactgga agaggtagag ttgtgctaag
      121 aggtaaatgc catgttgaaa caaaacaaag tggcagagaa caaattatta ttacagaagt
      181 gccataccaa gttggattag atgcattaac agataaaata ggacagctag taaatgataa
      241 aacaattgaa ggaattgccc atgtaaataa tgaaagcaat aaaagagaag gtattagaat
      301 tgttatagat ttaaaaagag atgcagtagc caatattata ataaatcaac tttacaagtt
      361 taccgaatta caaacaagtt ttggtattaa caatgttgca atcagcaaag gccgaccaag
      421 aatttttaat gtaaaagatt taattgtaga gtttgttgac tttagaatgg atgtggttat
      481 tcgccgttct aagtttgaac ttaaaaaggc agaagataga gcacatttac tagaaggata
      541 tttaaaagta attaatgata aagatcattt agacttggct atatccataa ttagaggcag
      601 tgcaacacca gatgaagcaa aaaaatcatt aatagaaaga agtatagcag ataattggta
      661 tttacccgat aacttatttg atgaaaaagc taaaattcat tatttacagg gtattggttt
      721 atccgaaaaa caagctcagg caattttaga attacgatta caaagattaa caggtttaga
      781 aagggagaaa attatagaag agtttaacga cctaatgaat accattgtga gattaaaaga
      841 tttattagca agtgaagact taagatatga tttaataaaa acagaattag aagaagtaaa
      901 agcaaagttt ggcgagccaa gaaaaagcga aattcaattt ttggcagatg aagtaaacat
      961 gttagatttt attaaagaag aagatgttgt aattactgtt tctcatttgg gttatattaa
     1021 aagaacttcc gcaaccgaat atcgtcaaca aaaaagaggt ggaagaggag caataggaag
     1081 taaaacaaga gaagaggatt ttgtagaaca cttatttgtt gcaagcacac acgatgtgat
     1141 gatgtttttt acagaaaaag gccgttgtta ttggttaaat gtttacgaaa tacccgaagg
     1201 agaaaaacaa agtaaaggaa gagcaattca aaatttaata caaataccac cagacgataa
     1261 aattaaagcc ataatagatg ttaaagattt agacaaaaaa gaaactttac aaaatagata
     1321 tgtagtatta tgtactaaaa aaggtattat taaaaaaaca gaccttgaag attttagcag
     1381 gccacgtacc acaggtatta atgcaattac catcttggaa ggcgatgaat tattagaagc
     1441 taaactaaca gatggaaatt gcgaaattat gatggctgtt aaaagtggaa gagctatacg
     1501 atttaatgaa gaaaaggtaa gagcaactgg tagaggagca attggtgtta caggaattga
     1561 agtagatgaa accaatgatg aagtagttgg tatgatttgt gttaataaaa acgatacaga
     1621 caaaacagtt ttagtaatta gcgaaaaagg atatggtaaa agaacaaaac taactgatga
     1681 agatggggaa gatgtatata gaataactaa cagaggaggc aaaggggtaa aaactattaa
     1741 tgtaacagaa aaaaccggaa gattagttgg cattttagat gtaaatgaaa aagaagattt
     1801 aattataact tgtcaaagtg gtattacctt gcgaacttcc atttcaacaa taaaaacggc
     1861 aggaagaaat acacaaggtg taattttaat aagattagat gcagaggatg ccattgcagc
     1921 tatttctaaa atagaagaaa cagaagaaga aacaactaca gaagtaaatg atcaatcaga
     1981 aaataattca ccagaaaata ccgataactt aaacactaat attgaaaaca gtaattaaaa
     2041 aaataactat gaaaaaaata atattacttg ttgcatgttc catttcattg gtaattgcca
     2101 atgcacaaga caaaatgtat aaaaaacttc aaaccttata cattgcagct caatacgaag
     2161 aagcaaaagc tgaaattgaa aaagtaatgg ccaatccaaa agcaatagat aaagcagaaa
     2221 catggttgtg gcgtacaaga atttatgccg aaatttactt ttcagaaccg ttaagaaaat
     2281 taaacccagg ctgtggaaat attgcctttg aatcttttaa aaaatatgaa gccttagaaa
     2341 gtaattataa aatgttggct aacccagaat tgtcagcttg gagatggaga cctttagatt
     2401 taatttatgt tactagtttt aatttaggcc gtagtttttt tgaagaaaaa aaatgggatt
     2461 cttcttacaa cacctttatg aaatcagttt acatgggtga tgtaattgtg aaaaataatt
     2521 tgagagagaa tggagcaaaa attgatacag tatctgtatt atacgcagct tatgcagcac
     2581 aaaatggaaa aatggaagca gaagctacag ccctatacga aaaatttgct gattgcaaaa
     2641 taggaggaaa ggattttaaa gatgcctata tgtacatttt attatacgca tctaaaacaa
     2701 aagataaagc caaattcaat aaatatattg ccattgcaaa agaggtttat ccaaatgatg
     2761 atgcttggga tgattatgaa atggaattta ttaccaacac cactacagtt gaagaaagaa
     2821 ttgcctatta tgataaagaa gatgcagctg gtactttaac tgcaagaaaa tatatgcttt
     2881 ttggccaatc ttttagcgaa gctacaaaag gagaagaagc agaaaaatta gacagtttaa
     2941 caaaagttag gtacgataaa aaagcagtag aagcttttaa aaaagcatat caaaaagata
     3001 ataaattagg cattgctgca tacaacgctg ggttacttta ttacaatgaa tttgttgaat
     3061 acgatgacag aataagtaac aacagaaggg ctttacagca attaaactct aacaaaccag
     3121 ttgtaaaaga tcctaaaaag aaagcagtag cagatgctga atttaaaaaa caaacagatg
     3181 ctattaaaaa attaaattca gatttagaga aaccaatttt agaaacttta gacagttgcg
     3241 tagtatggtt agaaatagct tacaataatt taaaaaacgg caatatggaa gatagaacaa
     3301 ctttgaactg tataaaaaat tcagtgaaat ggttggcaaa tgcttttatg tataaaagag
     3361 aaaaagtgaa aggaaaagat acaaaagctt atgatgctta cgatgcaaag tataatgagt
     3421 acgataaatt gtatgaaaaa tattaattaa aatttttcag tttaaataaa agcctaaact
     3481 atttgtttgg gcttttttac tcctatattg tacattttat gtttgcttaa atttatttta
     3541 tatattgcaa ttatgcaact aggaacagtt tatttaatac ctacagtttt atttgaggat
     3601 gaattacatt gcatacctga ctatgttgta gatactatta aaaattgcga aatttttttt
     3661 actgaaaatg aaaaaacaac ccgacgcttt tttaaaaagc tttggaaaga aatggtaata
     3721 gataattacc agtggcatac aattcataaa acagaacaag cagttgtaac cactttctta
     3781 caacatttaa aagatggaaa aaatgttgga attgttagcg aagcaggatg tcctggcatt
     3841 gcagatcctg gacaaatttt agtagaagca gctcaacaaa ttaacgcaat tattaaacct
     3901 ttggttggcc cttcatccat ttt
//
LOCUS       DAOQHP010000206         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14140_length_3922_cov_4.068787, whole genome shotgun sequence.
ACCESSION   DAOQHP010000206 DAOQHP010000000
VERSION     DAOQHP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14140_length_3922_cov_4.068787"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2291
                     /gene="acnA"
                     /locus_tag="PLY81_07145"
     CDS             <1..2291
                     /gene="acnA"
                     /locus_tag="PLY81_07145"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNF30040.1"
                     /translation="NNFTVVPPGMGICHQVNLEYLSKVTIERDGWLFPDTLVGTDSHT
                     PMVNGIGVIAWGVGGIEAEAAMLGQPIFFTCPEVVGLKLVGNIPNHCTATDMVLTITK
                     ILRDRGVVGKFVEVFGDGLNNLTVTDRATIGNMSPEFGCTVTYFPVDDRTLEYMKATN
                     RSSEQINIVEAYCKENLLWRTGNEKIKYSDIVELNLNTLEPTVSGPKRPQDKIFVKDL
                     SAKFSEILKNEFKREYENKNQRKEAAWLMEGGSGTEFVFGKVPIHGESHSEVVLDSKL
                     HSVRIKEKNKEFVVSDGSIVIAAITSCTNTSNPAVMVGAGLLAKKAVEKGLRTKSWVK
                     TSLAPGSKVVTKYLNRSGLSADLDALRFHTVGYGCTSCIGNSGPLPPAIAMAVDKGEL
                     VVASVLSGNRNFEARVHPQVKMNFLMSPMLVVAYALAGRVDIDLINEPIDYDPNGNPV
                     FLKDIWPSITEIQHTINECLKQDDFKEIYDEIFDGSADWQNLEINLNQSFNWNNTSTY
                     IKESPFFENLQATPNPITEITNARVLLYLGDSVTTDHISPAGSFKEDSAAGIYLKQNG
                     ILKDDFNSYGSRRGNHEVMMRGTFANVRIKNKIADKEGGFSKHFPTNQVKTVFETAMQ
                     YKEDQTALIVLAGKEYGSGSSRDWAAKGTFLLGIKAVIAESFERIHRSNLVGMGVAPL
                     TFLNNQNAELLGLDGSEVYTITGLADNLTPHKIVDVCAARADGKQINFKVKARLDSAI
                     EIEYYKNQGILQYVFREYLKNN"
     gene            2293..2736
                     /locus_tag="PLY81_07150"
     CDS             2293..2736
                     /locus_tag="PLY81_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF30041.1"
                     /translation="MNNKRITINKATPKAFDAMLVLEEFGKTCSIHPILKELIKIRAS
                     ILNGCAYCLDMHTEDAISLGENARRINAVSVWHESHLFNEEERTVLRITDEVTKIADK
                     GLTDSTYQKAIELFGESATAEIIMLIVTINAWNRIAVSTRLIYKP"
     gene            2761..3108
                     /locus_tag="PLY81_07155"
     CDS             2761..3108
                     /locus_tag="PLY81_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNF30042.1"
                     /translation="MIVIKRIYEHPSSKDGYRVLVDRLWPRGVSKQAAKIDLWCKEIA
                     PSPELRKWFAHDEKKWIGFKNKYTLELEQNIEQAEKLKILEKENKHLTLLYAAKNPLC
                     NHALVLDDFLSKL"
     gene            complement(3116..>3922)
                     /locus_tag="PLY81_07160"
     CDS             complement(3116..>3922)
                     /locus_tag="PLY81_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNF30043.1"
                     /translation="NSLLVPNQFLDGGVTGISLLLSHYFHAPLSIIIVLANIPFIILG
                     AFTAGKKFAIKTFVSIIVLGLCLQFAPISDITADWTGNMILVSLFGGFFIGLGVGLGM
                     RGGCSIDGIEILAIYTKRKIGFTMSELILALNVIIFLIAAFSFGMEKAFYAILTYYVA
                     TRTIGYVVEGIEEYTGITIISEHSESIKSLLVNELGKGISVYKGQRGFMKENFDVRYD
                     CDIVFTIVTRFEVRRVRNMVHLLDPKAFVFTHTIKETAGGVLKKVAADKH"
BASE COUNT         1409 a          605 c          715 g         1193 t
ORIGIN      
        1 taaacaattt tactgtagtg cctcctggta tgggcatttg ccatcaggta aatttagagt
       61 acttatctaa agttacaatt gaaagagatg gttggttatt tccagataca ttagttggaa
      121 ccgattctca tacaccaatg gtaaatggta ttggtgttat tgcatggggc gttggtggaa
      181 ttgaggcaga agcagccatg ctggggcagc caattttctt tacttgtccc gaagtggttg
      241 ggctaaaatt agtaggcaat attccaaatc attgtactgc aaccgatatg gttttaacca
      301 ttacaaaaat tttaagagat agaggtgtgg tgggcaaatt tgttgaagtg tttggagatg
      361 gcttaaataa cctaacagtt accgataggg caactattgg caacatgtct ccagagtttg
      421 ggtgtactgt aacttatttt cctgttgatg atagaacatt agaatacatg aaagctacca
      481 atagaagtag cgaacaaata aatatagtag aagcctattg taaagaaaat ttattatggc
      541 gtaccggtaa tgaaaaaata aaatattctg atattgttga attaaactta aacactttag
      601 agcctacagt atctggccca aaacgcccac aagataaaat ttttgtaaaa gatttaagtg
      661 ctaagtttag tgaaatttta aaaaatgaat ttaaacgtga gtatgaaaat aaaaaccaac
      721 gtaaagaagc tgcatggtta atggagggag gctctggaac tgaatttgtt tttggcaaag
      781 ttcctattca tggcgaaagc cattctgaag ttgttttaga tagcaagctt cactctgtaa
      841 gaataaaaga aaaaaataaa gaatttgttg taagcgatgg tagtattgta attgcagcta
      901 taacaagttg caccaatacc tctaaccctg cagtaatggt tggtgcaggc ttattagcta
      961 aaaaagctgt tgaaaaaggt ttaagaacaa aatcctgggt aaaaacttca ttagctccag
     1021 gctctaaagt ggttacaaaa tatttaaacc gctctggcct atcggcagat ttagatgcct
     1081 tacgttttca tactgtaggc tatggctgta cttcttgtat tggaaactcg ggccccctgc
     1141 ctccagctat tgcaatggct gtagataaag gcgaattagt tgtagcctct gttttatctg
     1201 gcaatagaaa ttttgaggca agagtgcacc cacaagtaaa aatgaatttc ttaatgtcgc
     1261 caatgctagt agtagcttat gctttagcag gaagggttga tattgattta ataaatgaac
     1321 ctatagatta cgacccaaat ggcaaccctg tatttttaaa agatatttgg ccaagtatta
     1381 cagaaattca gcataccatt aatgaatgtt taaagcaaga tgattttaaa gaaatttatg
     1441 atgaaatttt tgatggttct gccgattggc aaaatttaga aataaactta aatcaaagtt
     1501 ttaattggaa taatacatcc acttacatta aagagtctcc cttcttcgaa aatcttcaag
     1561 caacgccaaa cccaattacc gaaattacaa atgcaagagt attgctatat ttaggcgatt
     1621 cggtaactac agatcatatt tcgccagcag gctcttttaa agaagactct gcagcaggca
     1681 tatatttaaa acaaaacgga attttaaaag acgactttaa ttcctatggc tccagaaggg
     1741 gtaatcatga agtaatgatg agaggcactt ttgccaatgt acgaattaaa aataaaatag
     1801 cagataaaga aggtggcttt agtaaacact ttccaactaa ccaagtaaaa actgtttttg
     1861 aaacagctat gcaatataaa gaagatcaaa cagcacttat tgttttagca ggtaaagagt
     1921 atggctctgg ttcatcaaga gattgggcgg caaagggtac gtttttatta ggaattaaag
     1981 ctgtaattgc cgaaagtttt gaacgtattc atagaagtaa cttagtaggc atgggcgttg
     2041 ctcctttaac atttttaaat aatcaaaatg cagaattgtt agggttagat ggctctgaag
     2101 tttatactat tacaggctta gcagataatt taacccctca taaaattgta gatgtttgtg
     2161 cagctagggc agatggtaaa caaattaact ttaaagtaaa agctcgttta gattcggcta
     2221 tagaaattga atattataaa aaccagggca ttttacaata tgtatttaga gagtatttaa
     2281 aaaataatta aaatgaacaa caaaagaata actattaata aagcaacacc aaaggctttt
     2341 gatgctatgc tggttttaga agaatttgga aaaacatgct ccattcatcc tattttaaaa
     2401 gagttaataa aaattagagc atctatttta aatggctgtg cttattgttt agatatgcat
     2461 acagaagatg caataagttt gggagaaaat gctagaagaa ttaatgcagt aagtgtttgg
     2521 cacgaatcgc atttgtttaa tgaagaggag agaacggttt tacgaattac cgatgaagtt
     2581 accaaaattg ctgataaggg gcttacggat tctacctatc aaaaagctat tgaacttttt
     2641 ggagaatccg ccacagcaga aataattatg cttatagtaa ccattaatgc atggaataga
     2701 attgcagttt ctacgaggct tatttataaa ccttaattga gttttataaa ataataaact
     2761 atgattgtaa ttaaaagaat ttacgaacac ccttcttcta aagatggtta tagagttttg
     2821 gttgatagac tttggcctcg gggtgtatct aaacaagctg caaaaataga tttatggtgt
     2881 aaagaaatag caccaagccc agaattaaga aaatggtttg cacacgatga aaaaaaatgg
     2941 ataggtttta aaaataaata caccctggag ttagaacaaa atatagaaca agcagaaaaa
     3001 ttaaaaatat tagaaaaaga aaacaagcat cttacattat tatatgcagc aaaaaatccg
     3061 ctttgtaacc atgcacttgt attagatgat tttttaagta aactgtagag aaaaattaat
     3121 gtttatcggc agctactttt tttagcacac cacctgctgt ttcttttatg gtgtgtgtaa
     3181 atacaaaggc tttggggtct aataaatgta ccatatttct aactcttctt acttcaaaac
     3241 gggttacaat ggtaaaaaca atatcacagt cgtatctaac atcaaagttt tctttcataa
     3301 accctctttg acctttatat acagatatgc ctttccctaa ttcatttact aaaagggatt
     3361 ttatactttc gctgtgttca gaaataatag taatgccagt atattcttct ataccttcaa
     3421 ccacataacc aatagttctt gttgcaacgt aataagtaag aattgcataa aaagcttttt
     3481 ccataccaaa gctaaaggcg gctattaaaa atataattac attaagtgca agtattaatt
     3541 cactcattgt aaatcctatt tttctcttgg tataaatggc taaaatttca attccatcta
     3601 tagagcagcc accacgcatt cctaagccaa ctccaagacc aataaaaaaa ccaccaaata
     3661 aagaaactaa aatcatgtta cctgtccagt ctgctgtaat atcactaatg ggtgcaaact
     3721 gaaggcataa gcctaaaaca ataatgctta caaatgtttt gatagcaaat tttttaccag
     3781 cagtaaatgc ccccagaata ataaatggaa tattagccaa tactattata atactcaatg
     3841 gtgcatgaaa gtaatggctt aataaaaggc taatacctgt aacaccacca tctaaaaact
     3901 ggttgggtac tagtaaacta tt
//
LOCUS       DAOQHP010000207         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14156_length_3920_cov_3.845278, whole genome shotgun sequence.
ACCESSION   DAOQHP010000207 DAOQHP010000000
VERSION     DAOQHP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14156_length_3920_cov_3.845278"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLY81_07165"
     CDS             complement(<1..1209)
                     /locus_tag="PLY81_07165"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNF30044.1"
                     /translation="MYPNLYYFFKEVFGIEIEGFKIINSFGFFVAIAFIGAAWALTQE
                     LKRKQKQGLFVYTEEEIIVGKPASASELMIHFFLGFIFGFKILGGFFLPNAFSDPQLY
                     IFSGQGNWWMGLLVGGVFAWIKYNEKNKLKLPTPEKRTIRIWPHDRVGDLVIYAAIFG
                     FAGAKLFDILEAPSAFFSMFKAVKEGKQDVGSLLFSGLTFYGGLIVATIAIIVYAKKH
                     KIGIIYLADAFAPAMMLAYALGRIGCQVAGDGDWGIVNSAFISNNIGESILATPEQFT
                     NALHTHSAYLSSKFGTIEQMHHMSIQPFLGLPNWMFGYTYPHNVVGDGVALANCSGVH
                     CNYLPLPVFPTPFYELTACLILFLILWAVRSKLKIPGQLAGLYLIFNGTERFFIEKIR
                     VNATYNLAGMK"
     gene            1334..>3920
                     /locus_tag="PLY81_07170"
     CDS             1334..>3920
                     /locus_tag="PLY81_07170"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF30045.1"
                     /translation="MKNRIVLLLLMLFCVINSFSQNNYWQQKVDYNLKVSLNDTANTL
                     DGFATITYYNNSPDTLQFIWFHLWANAYKNDKTAYTKQALENGSTTFYFSNDEDRGYI
                     NQLNFKVNNVTALTEVHQQYTDIIKLILPSALPPQQSITITTPFFVKIPALFSRMGVS
                     KQNYFITQWYPKPAVYDRKGWHEMPYLDQGEFYSEFGDFDVQIILPDSYIVAATGELQ
                     QTDLLHTLKKLGKEKPEKQANYLNYLKEEKQKKISPSKTSKVTATKHSPTLTAYNFKQ
                     TNCHDFAWFASKNFILQYDTVKLTTKTVDVFTFYLPQQLKAWNKSIAYAKNGIKKYSN
                     WVGEYAYNTVSVVAGFENEFSGGMEYPTITVITTNTEGKRLDETIVHEIGHNWFYGVL
                     ASNERKHAWMDEGMNTFYEKKYMKEQYASNNKSLLSNYSIDVLEKVANRTITKIKKSQ
                     PIDTISTAYSNLNYFLIVYSKASSWMKQLETTLGEDSFKEVMQQYYNDWKFKHPYPED
                     FKKIVINKNKEAEFLFSYLYNKKNLDTVSYNKKIKPIFIASLKETEKYNYIGISPLAA
                     YNYYDKVSIGAFIHNYQLPLNRFQFFVAPLYAFGSKEFNFNARASFNTYKEKYWLEFA
                     TSASKFTIDNFKPTNAATINQQVVKIVPSVKYTLYRNDKRSTLRWVFQARSFLLQENG
                     LLFKTLNGVDIVEKTTENSYINQFKITTLDNRILYPYNFNLTIDQGKSFVRAGFTANY
                     HFNYKNRKGGIDARFFAGKFFYTSDKTFIKQYETDRYHLNMSGAKGYEDYTYSNYFIG
                     RNEFEGWQSQQIMQRDGFFKVSTDLLSNKIGKTDDWLMALNLSGNIPDAINILNVLPI
                     KIPLKF"
BASE COUNT         1474 a          572 c          592 g         1282 t
ORIGIN      
        1 tttcattcct gctaaattat aggttgcatt cactcttatt ttttctataa aaaacctttc
       61 ggttccattg aaaataagat ataagcctgc tagctgtcct ggtattttta acttacttct
      121 tactgcccaa agaattaaaa ataaaattaa acaagctgtt aactcataaa aaggagttgg
      181 aaaaacaggg aggggtaaat aattgcaatg tacaccggaa cagttggcta atgcaacacc
      241 atcgcctaca acattatgag gataagtata accaaacatc caatttggta aacctaaaaa
      301 aggctgaata ctcatatggt gcatttgttc aattgttcca aacttactgc ttaaataagc
      361 actatgagtg tgtaatgcat ttgtaaattg ttcgggtgtt gctaaaatgc tttctccaat
      421 attattacta ataaaagcac tgttaacaat accccaatct ccatcaccag caacctggca
      481 accaattctt cctaaagcat aagccaacat catagcaggt gcaaaagcat cggctaaata
      541 aataatgcct attttatgtt tttttgcata aacaataatg gcaatggtgg caacaattaa
      601 accaccataa aaagttaacc cactaaatag taaagagcct acatcttgtt taccttcttt
      661 taccgcttta aacatagaaa aaaatgctga tggggcttct agaatatcaa ataatttagc
      721 accagcaaaa ccaaatatgg ctgcataaat tactaaatca cctacacgat catgaggcca
      781 aattctaata gttctctttt caggtgtggg taattttagt ttattttttt cattgtattt
      841 tatccatgca aaaacgccac caactaataa acccatccac caatttcctt gaccagaaaa
      901 aatatatagt tgcggatcgc taaaagcatt tggtaaaaaa aagcctccta atattttaaa
      961 cccaaatata aaacctaaga aaaaatgtat cattaattca gatgcagagg caggcttgcc
     1021 tacaattatt tcttcttcgg tataaacaaa taagccttgt ttttgcttac gtttaagctc
     1081 ttgtgttaaa gcccatgcag cacctataaa agctattgct acaaaaaagc caaatgaatt
     1141 gatgatttta aagccttcta tttctatacc aaaaacttct ttaaaaaaat aatataaatt
     1201 agggtacata ctactgattt atgttgcaat gttaaacaga aataactaat atgtttacta
     1261 ataattatag tttgatttga atagcctatt ttgtgtaaaa tatttatata tcttcaacca
     1321 aatttttaga agggtgaaaa atagaattgt tctactatta ctaatgttgt tttgtgtaat
     1381 aaattccttc tcgcaaaaca attattggca gcaaaaagta gattataatt taaaagttag
     1441 tttaaatgat actgcaaata ctttagatgg ttttgccact attacttatt ataataattc
     1501 gccagatact ttacaattta tttggtttca tttatgggct aatgcctata aaaatgataa
     1561 aactgcttat actaaacaag cattagagaa cggaagtaca acattttatt ttagtaatga
     1621 tgaggataga ggctatatta atcagctaaa ctttaaagta aataatgtaa ctgcattaac
     1681 tgaagtgcat caacaatata ccgatataat aaaattaatt ttaccaagtg cattacctcc
     1741 tcagcaatct attaccatta ctactccctt ttttgtaaaa attcctgctt tgttttcaag
     1801 aatgggtgta agtaagcaaa attattttat tacacaatgg tatcctaagc cagcagttta
     1861 tgatagaaaa ggttggcacg aaatgcctta tttagaccaa ggcgaatttt atagtgaatt
     1921 tggagatttt gatgttcaaa ttattttacc cgattcttat attgttgctg caactggtga
     1981 gttacaacaa actgatttgt tacatacttt aaagaaacta ggaaaagaaa aaccagaaaa
     2041 acaagccaat tatttgaatt atttaaagga agaaaagcaa aaaaaaatat ctccctctaa
     2101 aacctctaaa gtaacagcta ccaaacatag cccaacatta actgcctata actttaaaca
     2161 aactaattgc catgattttg catggtttgc ttcgaaaaat tttattctgc aatatgatac
     2221 tgttaaactt actactaaaa ctgtagatgt atttactttt tatttaccac agcagttgaa
     2281 agcatggaat aagagtattg cttatgctaa aaatggtata aaaaaatata gtaattgggt
     2341 tggagaatat gcttataata cagtatctgt tgttgctggg tttgaaaatg aatttagtgg
     2401 tggaatggaa tatcctacaa ttactgtaat tacaacaaat actgaaggga aaaggttaga
     2461 cgaaactatt gtacacgaaa ttggacataa ctggttttac ggggtgttgg caagcaacga
     2521 aagaaaacat gcgtggatgg atgaaggcat gaatactttt tatgaaaaaa aatatatgaa
     2581 ggaacaatat gctagtaata ataaaagttt gcttagcaat tattctatag atgtgcttga
     2641 aaaagtagcc aatagaacta ttaccaaaat taaaaaatct caaccaattg atacaattag
     2701 tacagcttat agtaacctta attatttttt aattgtttat tctaaggcaa gcagttggat
     2761 gaaacaatta gaaacaactt taggagaaga tagttttaaa gaagtaatgc aacaatatta
     2821 taatgattgg aaatttaaac atccttatcc tgaagatttt aaaaaaatag ttattaataa
     2881 aaacaaagaa gcagagtttt tgttttctta tttatacaat aaaaaaaatt tagatacagt
     2941 aagttataat aaaaaaatta aaccgatttt tatagcctct ttaaaagaaa cagaaaagta
     3001 taattatata ggtatttcgc ctttggctgc atataattat tacgataagg tttctattgg
     3061 tgcttttatt cataattatc agttaccgct caatcggttt caattttttg ttgcaccttt
     3121 gtatgccttt ggcagtaagg agtttaactt taatgcaaga gcaagtttta atacttataa
     3181 agaaaaatat tggctagaat ttgctacgag tgcatcaaaa tttacaatag ataattttaa
     3241 accaactaat gctgcaacaa ttaatcaaca agttgtaaaa attgttccat cagttaaata
     3301 cactttgtat cgcaatgata aaagaagtac actgcgttgg gtgtttcaag caagaagttt
     3361 tttattacaa gaaaatggct tgttgtttaa aacgttaaat ggtgttgata ttgtagaaaa
     3421 aacaactgaa aacagttata taaatcaatt taaaataaca acattagata acagaatact
     3481 gtatccttac aattttaatt taaccataga ccaaggtaaa agctttgttc gtgctggttt
     3541 tacggcaaat tatcatttca attataaaaa tagaaagggc ggaattgatg caagattttt
     3601 tgccggaaaa ttcttctaca cctccgataa aacatttatt aaacagtacg aaacagatag
     3661 atatcattta aacatgagtg gtgctaaagg gtatgaagat tatacttaca gtaattattt
     3721 tattggcaga aatgaatttg aaggttggca aagccaacaa ataatgcaac gtgatggttt
     3781 ttttaaggta agtacagatt tgttaagtaa taagattggc aaaacagatg attggttaat
     3841 ggctttaaat ttaagtggta atattcctga tgcaattaat attttaaatg ttttgccaat
     3901 aaaaattcct ttaaagtttt
//
LOCUS       DAOQHP010000208         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14227_length_3909_cov_3.911261, whole genome shotgun sequence.
ACCESSION   DAOQHP010000208 DAOQHP010000000
VERSION     DAOQHP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14227_length_3909_cov_3.911261"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLY81_07175"
     CDS             complement(<1..395)
                     /locus_tag="PLY81_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF30046.1"
                     /translation="MKLLSPAISRLARLRYWRIEQWLQNPFAAQREVLQDLVTHAQYT
                     EIGRKYGLTNLFKIRDFKKAVPIHTYDDLKPYIERLMQGEQNLLWNTPVYWFAKSSGT
                     TSDKSKFIPVTEESLTNCHYQGTKDVLST"
     gene            complement(547..1311)
                     /gene="lptB"
                     /locus_tag="PLY81_07180"
     CDS             complement(547..1311)
                     /gene="lptB"
                     /locus_tag="PLY81_07180"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNF30047.1"
                     /translation="MSLTIHTNNLIKTYKSRTVVNNVSIKLQQGEIVGLLGPNGAGKT
                     TTFYMVVGLIKPDDGNVLLNDSNITKLPMYKRAQMGIGYLPQEASVFRKLSVEDNIMS
                     VLEMTKKNKTQRKEKLEELLDEFHLHHVRKNNGDSLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGVDPIAVEDIQSVVAKLKYKNIGILITDHNVNETLSICDRAYLLIDGKIFKH
                     GTAEELANDDQVKRLYLGTNFELKRKDWIIEMGNSQ"
     gene            complement(1385..1924)
                     /gene="rsmD"
                     /locus_tag="PLY81_07185"
     CDS             complement(1385..1924)
                     /gene="rsmD"
                     /locus_tag="PLY81_07185"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNF30048.1"
                     /translation="MRIISGKFGGRKINPPSKMPHTRPTTDIAKEGLFNILQNRISFD
                     QIKTLDLFGGTGSISYELASRGASHLTIVEKDAVMYNFIKNNIAQLKIENCQVFKLDI
                     FNFLATCTEKYDFIFAGPPYALGSIDEIPKIIMQKQLLAPNGYFVLEHTPRNNYETFT
                     GFSFVRNYGTTLFSFFIAE"
     gene            complement(1921..2775)
                     /locus_tag="PLY81_07190"
     CDS             complement(1921..2775)
                     /locus_tag="PLY81_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNF30049.1"
                     /translation="MVQKLVAFYGDTNTYTHVNKNNFFIEASNGHYVCWVRTEKDNSI
                     AAVEVFTVEDEANFDWNDIFYEIGKQSSLHDKSYNLVTIFYHNNENVLVPAHKFNASN
                     ADAYLDLMFGAKENTVVKYDSVDFGGELMYNVYRVNALLNTMVKTNFLTVTEFHHYSG
                     LLKHLTIEDKKLQGNNINIYFYHQSFSVIVLKNNKLQIAQSFKFSCPEDVLYYCLNLN
                     NTFKLSTQADPIIISGFIEQNGAIINLLNQHFSNVLIASPNADEMAFETNAQFPAYYF
                     TPFFNLQV"
     gene            complement(3097..>3909)
                     /locus_tag="PLY81_07195"
     CDS             complement(3097..>3909)
                     /locus_tag="PLY81_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNF30050.1"
                     /translation="IKIIARKVTKKNLRIKLKLTFAEIANGVTKQVKVKKYIICTTCG
                     GNGAKDKNSLQTCTTCGGNGQVRKVQNTFLGQMQTVTTCSTCNGEGSTVTSKCTACKG
                     EGRVYGEETISIDIPAGVQAGMQLSMSGKGNAGERGGSYGDLIIQIEEEQHTDLQRDG
                     LNVAYDLHISFPEATFGTNIEVPTIDGRAKIKIPPGTQSGKIFRLKGKGFPEVQGYGR
                     GDQLVNVNVWTPQNLTSEEKAILEKLNNSPNFKPQPSKNDKSFFEKIREAFN"
BASE COUNT         1243 a          627 c          559 g         1480 t
ORIGIN      
        1 taagtactta atacatcttt tgtaccttga taatggcaat tggtaaggct ttcttcagtt
       61 acaggaataa acttactttt atcgctggtt gtaccactgc tttttgcaaa ccaataaaca
      121 ggtgtgttcc aaagtaaatt ttgttcgcct tgcattagcc tttctatata tggcttaaga
      181 tcatcgtatg tatgaattgg aactgctttt ttaaagtctc ttattttaaa taaattggtt
      241 aacccatact ttctgcctat ctctgtatat tgggcatggg ttactaaatc ttgcaatact
      301 tctctttgtg ctgcaaaggg gttttgcagc cattgctcta ttctccaata acgtaaacga
      361 gcaagccttg atatggcagg actaagcagt ttcattttaa aaataacgtt agcaagttaa
      421 gcaatgataa gaaaaaaata cttgaaagtg atattttttt gaaaattaag aaggaaggaa
      481 aattgtaaaa taatattaag gagaatgaaa ctgaaaatca atggtaatat gaaagtaaat
      541 aatttattat tgggaatttc ccatttcaat tatccaatct tttcgtttta attcaaaatt
      601 agttccaagg tataaacgtt ttacctgatc atcatttgcc aattcttcgg cagtgccatg
      661 tttaaatatt ttaccatcaa ttaataaata ggctctatcg caaatagaaa gggtttcgtt
      721 aacgttatgg tcggtaatta atattcctat atttttatac ttcagctttg caactacact
      781 ttgtatatct tctacagcaa ttgggtcaac acctgcaaaa ggttcatcta ataaaataaa
      841 tttaggatcc actgctaatg ctcttgcaat ttctgttctt cttctttccc caccacttaa
      901 actatctcca ttattttttc ttacatgatg taagtgaaac tcatctagca attcttctaa
      961 tttttctttt cgttgcgttt tatttttttt agtcatttct aatacactca taatgttgtc
     1021 ttcaacagat agttttctaa aaacacttgc ctcttgtggt aaataaccta tgcccatttg
     1081 ggcacgtttg tacattggta attttgtaat attactatca tttaataaca cattcccatc
     1141 atctggtttt attaagccaa ccaccatata aaaagtagtt gttttaccag caccatttgg
     1201 accaagtaag ccaactattt cgccttgttg taattttatg ctaacgttat ttacaacagt
     1261 tctgctctta tatgttttta ttaaattgtt ggtatgaatt gttaggctca atgtaaaaga
     1321 tttaaacaaa aataaagtta atgtacatga tattttattt taaaatattt tatttaagat
     1381 cattttattc agcaataaaa aagctgaaca atgtagtgcc ataatttctt acaaaagaaa
     1441 agcctgtaaa tgtttcataa ttgtttcttg gggtatgttc taacacaaaa taaccattgg
     1501 gtgcaagtag ctgtttttgc ataattattt taggaatttc atcaatagag cctaatgcat
     1561 aaggtggacc agcaaaaata aaatcgtact tttctgtaca ggttgctaaa aaattaaaaa
     1621 tatctaactt aaaaacctgg caattttcaa tttttaattg tgcaatatta ttttttataa
     1681 aattgtacat aacagcatct ttttctacaa tggttaaatg gctagcacca cgtgatgcta
     1741 attcgtaact aatacttcct gtaccaccaa aaagatctaa tgtttttatt tgatcgaaag
     1801 aaattctatt ttgcagaata ttaaataatc cttctttggc aatatctgtt gtaggacgag
     1861 tatgaggcat ttttgaaggt ggatttattt ttcttcctcc aaattttcct gaaattattc
     1921 tcatacttgt aaattaaaaa atggggtaaa ataatatgct ggaaattgtg cattagtttc
     1981 aaatgccatt tcatcggcat tgggagatgc aattaataca tttgaaaaat gttggtttaa
     2041 taaatttata atggcaccat tttgttcaat aaacccagat ataattattg gatcggcttg
     2101 tgtagaaagt ttaaaagtat tatttaggtt taagcaatag tacaacacat cttcggggca
     2161 gctaaactta aagctttgtg caatttgtag tttattattt tttagtacaa taacagaaaa
     2221 tgattgatgg tagaaataaa tatttatatt attaccctgt agctttttat cttcaatggt
     2281 taagtgttta agtaacccag agtagtggtg aaattcggta actgttaaaa aattagtttt
     2341 aaccatagta tttaataatg catttactct gtacacattg tacataagtt cacctccaaa
     2401 atcaacacta tcatatttta ctacagtatt ttctttagct ccaaacatta aatctaagta
     2461 tgcatcggcg ttagatgcat taaatttatg tgcaggcact aaaacgtttt cgttattatg
     2521 gtaaaaaatt gttacaaggt tatagctttt atcatgtaac gatgactgtt tgcctatttc
     2581 ataaaaaata tcattccagt caaaattcgc ttcatcttca acagtaaata cttctactgc
     2641 agcaatacta ttatcctttt ctgttcttac ccaacatacg taatgcccat tacttgcctc
     2701 tataaaaaag ttatttttat ttacatgagt gtaggtattt gtatcaccat aaaaggctac
     2761 taatttttgt accataatat gctattgcta tgcaattata taaaataaat actgtattag
     2821 aaaaggctaa agtacaaata atataatagg aagagtttga aaataagtca atatactttg
     2881 attttttgat tttttgaaga atagtattta aagtttaggg gcaggtttat actattgata
     2941 tttaaaaatt aaaacagtta aatagtatta tttccttttt ataaattgct tcatgggctt
     3001 tacaactaat tagtttaaag taaagccata attcatatct atattaaaat ccagaggagg
     3061 aaacattaaa aaagcctttt acaaggctta aaaatgttaa ttaaatgctt cccgaatttt
     3121 ttcaaaaaaa cttttatcgt ttttagaagg ctgaggttta aaattagggc tattgtttaa
     3181 tttttctaat atcgctttct cttcagatgt taagttttga ggagtccaaa catttacatt
     3241 tactaattgg tcgcctctac cataaccttg cacttcagga aatcctttcc ctttaagcct
     3301 gaaaattttg ccactttgtg tacctggcgg aatttttatt tttgctcttc cgtcaatagt
     3361 tggaacttct atattggttc caaaagttgc ttcaggaaat gagatatgta aatcgtatgc
     3421 aacgtttaat ccatctcttt gtaaatcggt atgttgttct tcttctattt gaataattaa
     3481 atcgccgtaa gaccctcctc tttctccagc attgccttta ccactcatac taagttgcat
     3541 acctgcttgt acgcctgctg gtatatcaat actaattgtt tcttctccat atactctccc
     3601 ttctccttta catgctgtac attttgaagt aactgtagag ccttctccat tacatgtaga
     3661 gcaagtggtt acggtttgca tttgacctaa gaaagtattt tgcactttac gaacttgtcc
     3721 attaccacca caagttgtac atgtttgtaa actattttta tcttttgctc cattgccacc
     3781 acaagttgta caaataatat atttttttac cttcacttgt ttggttacac cgtttgcaat
     3841 ttctgcaaaa gtgagcttta attttattct taaattcttc tttgttacct ttcgagctat
     3901 tattttaat
//
LOCUS       DAOQHP010000209         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14252_length_3906_cov_2.719294, whole genome shotgun sequence.
ACCESSION   DAOQHP010000209 DAOQHP010000000
VERSION     DAOQHP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_3906_cov_2.719294"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLY81_07200"
     CDS             <1..235
                     /locus_tag="PLY81_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNF30051.1"
                     /translation="ILATNALVQGGNMPCILNAANEIAVFAFLRNRIGFLDITEMVER
                     TINKIPFIANPTLEELFVSDGEARNFAASLVEL"
     gene            321..1679
                     /gene="rseP"
                     /locus_tag="PLY81_07205"
     CDS             321..1679
                     /gene="rseP"
                     /locus_tag="PLY81_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNF30052.1"
                     /translation="MELIAVDWNGGLIQAGQFVLSFSILVVLHELGHYFPAKWFKCKV
                     EKFYLFFNPWFSLFKKKIGETEWGIGWVPFGGYVKIAGMIDESMDKEQMKLPPKPNEF
                     RAKPAWQRLIIMIGGVVVNILLAIIIFIGISWYWGEEKLQGKNLKYGIVADSIGKMIG
                     LKDGDVIYKVGSEDVTDAIDRKIASQIILSNASNISIVRNGENMQLNVTEEFLTKLKK
                     NKGNGIASIRIPFIIDTVMAGQGAAKAGLKKNDYIIAVDSIATPYFNDFQNVIVNYAS
                     KTVPFKVIRNKDTLTLPVTLSDNAKIGAGTKGLKDLGFEIDVKNYSLLEAIPVGFNLC
                     WSSLDKYIEGIKRLFQGKDKVSDSLGSVISIGSIYSKVWDWQNFWTLTALFSIILAFM
                     NILPIPALDGGHALFCIVEMVTGKKPNDKFMEYAQTVGMFLLLGLMVYALGLDFLRLF
                     KG"
     gene            1771..2143
                     /gene="ssrA"
                     /locus_tag="PLY81_07210"
     tmRNA           1771..2143
                     /gene="ssrA"
                     /locus_tag="PLY81_07210"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2153..3088)
                     /gene="fmt"
                     /locus_tag="PLY81_07215"
     CDS             complement(2153..3088)
                     /gene="fmt"
                     /locus_tag="PLY81_07215"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNF30053.1"
                     /translation="MNYKDLRIVFMGTPEFAVASLNALVNAGCNIVAVVTAPDKPAGR
                     GLQLNESAIKKYAVANNLKILQPVKLKSVDFIETLKSLKADVQIVVAFRMLPEMVWNM
                     PTMGTINVHGSLLPQYRGAAPINWAVINGEKFTGVTTFKLKHAIDTGNILLQESLAID
                     ENETAGEVHDKMKELGAELLLKTIQQLAEGKLIEQEQNKLNNTLTLKHAPKLYSENGK
                     IDFSKTVEEVHNLVRGLSPFPGAYTFLNNKLLKIYRTIKEVSDEPLEVGSIKSDGKKY
                     FKMACCNGYINILELQLEGKKRMKIEDFLRGNKIQ"
     gene            complement(3128..3349)
                     /locus_tag="PLY81_07220"
     CDS             complement(3128..3349)
                     /locus_tag="PLY81_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30054.1"
                     /translation="MQKIKKYLGIVWIILAILSVIILIITAIENIASKGGADIHNPIP
                     WIIIITIYLPIAFGFALFGYYAFKEEYHV"
     gene            complement(3359..>3906)
                     /locus_tag="PLY81_07225"
     CDS             complement(3359..>3906)
                     /locus_tag="PLY81_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF30055.1"
                     /translation="IFSELYSITNTKNKQEITTNIHISKTVVLNNTADSLITKTTTTN
                     FTDKTVLIETEKYVESVVKTGNNQPKESKKIITLDSHYKWKIIGLIFILVFFVTMVYG
                     PIAAFLVELFPTKIRYTSMSLPYHIGNGVFGGLVPFIATLITVMPGSNYLSGLWYPIG
                     IGLICFIIGLVYLSNNQVKNS"
BASE COUNT         1313 a          549 c          640 g         1404 t
ORIGIN      
        1 aatactggca actaatgctt tagtacaagg tggcaacatg ccatgtattt taaacgcagc
       61 caatgaaata gctgtatttg ccttcttacg caacagaatt ggctttttag atattaccga
      121 aatggtagaa agaactatta acaaaatacc ctttattgct aatcccacgt tagaagaatt
      181 gtttgttagc gatggtgagg cacgtaattt tgcagcctct ttagtagaat tataaatttt
      241 taaaactatt gctacaaaag attttctcaa aaaaaatatt ttttacaata acaaaatata
      301 aaataaaagt ttaaataatt atggaattga tagcagttga ttggaatggt ggtttaatac
      361 aagcagggca gtttgtatta tcgttttcaa ttttagttgt gttgcacgaa ttaggtcatt
      421 attttcctgc aaaatggttt aaatgtaaag tagaaaaatt ttatttattc tttaaccctt
      481 ggtttagttt atttaaaaag aaaattggtg aaacagaatg gggtattggc tgggtgcctt
      541 ttggtggcta tgtaaaaatt gctggaatga ttgatgaaag tatggataaa gagcaaatga
      601 aattaccacc taaaccaaat gaatttaggg caaagccggc ttggcaaaga cttatcataa
      661 tgattggtgg agtggttgta aatattcttt tagcaataat tatttttatt ggaataagtt
      721 ggtactgggg cgaagaaaaa ttacagggca aaaatttaaa atacggaatt gttgctgatt
      781 caattggtaa aatgattggt ttaaaagatg gagatgttat ttataaagta gggtcagaag
      841 atgttacaga tgctatagat agaaaaattg caagccaaat aatattaagt aatgcctcca
      901 atatttctat agttagaaat ggtgaaaata tgcaattgaa tgtaaccgaa gaatttttaa
      961 ctaaattaaa aaagaataaa ggcaatggca tagcaagtat tcgtattcca tttataatag
     1021 atacagtaat ggcaggccaa ggtgcagcaa aagctgggtt aaaaaagaat gattatatta
     1081 ttgcagtaga tagtattgca acgccatact ttaacgattt tcaaaatgta attgtaaact
     1141 atgcctctaa aacggttcca tttaaagtaa ttagaaataa ggatacttta accttgcctg
     1201 taacacttag cgataatgca aaaattggtg caggaactaa agggttaaaa gatttaggct
     1261 ttgaaataga tgtgaaaaat tattcgttat tagaagctat acctgttggc tttaatttat
     1321 gttggtcaag tttagacaaa tatattgaag gaattaaaag actatttcaa ggaaaagata
     1381 aagtaagtga tagcctaggt agtgtaataa gtattggtag tatttatagc aaagtgtggg
     1441 attggcaaaa cttctggact ttaactgcac ttttttctat catactagca tttatgaata
     1501 ttttacctat acctgcttta gatggtggcc atgcattatt ttgtatagtt gaaatggtta
     1561 caggaaaaaa acctaacgat aaatttatgg agtatgcaca aactgtaggc atgtttttat
     1621 tattaggttt aatggtgtat gcattaggtt tagatttttt aagattgttt aaagggtagg
     1681 ttatttaaat aaaaaaatta gttgatattg gaatgttttt tgtaacaatt ctaccttaaa
     1741 attattgttg ttacagtaaa ataaatgttt ggggccgtat tggttttgac agcaaagttc
     1801 tttgtaagta taagcatgca gtgcgttgta taattagcac tttaatctga atattcaaac
     1861 tttaaatggc aatactcagt atgccatggc tgcctaatca gtgattagtt agtcatttgg
     1921 gccgccgtac aggtttactt tttaccaagt accgccgccg actcaaaaca aataaaagtt
     1981 gcttttgcta aaggctgtga tagtaaatta agatcattca gctaaggata atattggttg
     2041 gtgctgcccc tgtattttcc ctacaattaa tgcagtaata agcatgtaga aaacttatgg
     2101 cgaatttgtt tggacaaggg ttcgactccc ttcggctcca ctttttttat gcttattgaa
     2161 ttttatttcc tctcaaaaaa tcttctattt tcattctttt tttaccttct aactgtaact
     2221 ctaaaatatt tatataccca ttacaacaag ccattttaaa atattttttt ccatcgcttt
     2281 ttatgctacc tacctctaat ggttcatcac ttacttcttt gattgttcta taaattttta
     2341 ataatttatt atttaaaaaa gtatatgcac caggaaaagg agataagcct ctaaccaaat
     2401 tatgtacttc ctccactgtt ttagagaaat ctattttccc attttcagaa taaagctttg
     2461 gtgcgtgttt caaggttaat gtattattca atttattttg ttcttgttca attaatttgc
     2521 cttctgcaag ttgttgtatt gtttttaata acaactctgc tccaagttct ttcattttat
     2581 catgcacttc tcctgcagtt tcattttcat ctatggctaa actttcttgt aataaaatat
     2641 tgccagtatc tattgcatgt tttagtttaa atgtagtaac gcctgtaaac ttttctccat
     2701 taataactgc ccagtttatt ggtgctgccc ctctgtattg aggcagtaaa cttccatgta
     2761 catttatggt tcccatagta ggcatattcc aaaccatttc aggtaacatt ctaaaggcta
     2821 ctacaatttg tacatctgct tttaaacttt ttaatgtttc aataaaatct acacttttta
     2881 attttacagg ttgtaatatt ttaagattat ttgcaacagc atattttttt atagcacttt
     2941 cattaagctg caacccacgt ccagcaggtt tatcaggtgc agttacaaca gcaacaatat
     3001 tgcaacctgc atttaccaaa gcatttaacg atgctacagc aaactctggc gtacccataa
     3061 aaacaattct taaatcttta taattcatgc agcaaaagta aagcagctat taatactagt
     3121 cttataatta tacatggtat tcttctttaa atgcataata accaaatagg gcaaaaccga
     3181 aagcaatggg taagtaaata gtaattatta ttatccaagg tattggatta tgaatatctg
     3241 caccaccttt tgaggcaata ttttcaattg cagttattat taaaataatt acagaaagta
     3301 ttgcaagtat tatccaaact atgcctaaat atttttttat tttttgcata aattaaaatt
     3361 atgaattttt tacttgattg ttgcttaaat acactaaccc aataataaaa caaattaatc
     3421 caataccgat tgggtaccat aagccactta aataattact accaggcata acagttatta
     3481 atgttgcaat aaaaggtaca agcccaccaa aaacaccatt accaatatga taaggtaaag
     3541 acattgaagt atatcttatt ttagtaggaa agagttctac taaaaatgct gcaattgggc
     3601 cgtaaaccat ggttacaaaa aataccaaaa taaatattaa accaattatt ttccatttat
     3661 aatggctatc cagtgtaatt atttttttag attctttggg ttggttattt ccagttttta
     3721 ctacagattc tacatatttt tctgtttcaa ttagtacagt tttatcagta aaatttgtag
     3781 ttgttgtttt agttattaac gaatcggcag tgttatttaa aactactgtt ttgcttatgt
     3841 gaatatttgt ggttatttct tgtttgtttt ttgtattagt gatagagtat aattcagaaa
     3901 atatag
//
LOCUS       DAOQHP010000210         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14304_length_3897_cov_3.447683, whole genome shotgun sequence.
ACCESSION   DAOQHP010000210 DAOQHP010000000
VERSION     DAOQHP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14304_length_3897_cov_3.447683"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="ispG"
                     /locus_tag="PLY81_07230"
     CDS             complement(<1..827)
                     /gene="ispG"
                     /locus_tag="PLY81_07230"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNF30056.1"
                     /translation="MQLYCNSLTNYKRLLTKEVKIGDLLLGNNHPIRLQTMTTTDTMN
                     TIATVEQTIRCIEAGAELVRITAPSIKDAENLLHIKNELHKRGFKIPLVADIHFTPKA
                     AEISARIVEKVRVNPGNYVDKKKFEQIEYTDAEYLEEIERIRERFTPLIKICREYGTA
                     MRIGTNHGSLSDRIMSRYGDTPMGMVESAMEFLRIARNESYHNILLSMKASNPQVMVQ
                     AYRLLIQQMETEFGECYPLHLGVTEAGDGEDGRVKSAAGIGTLLEDGIGDTIRVSLT"
     gene            993..1715
                     /locus_tag="PLY81_07235"
     CDS             993..1715
                     /locus_tag="PLY81_07235"
                     /inference="COORDINATES: protein motif:HMM:NF021541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HNF30057.1"
                     /translation="MKLLVLVYMLVWCGTLVAQTERSDSLLNFKNNLLQIVQEEYCFW
                     NDPVNRVETQFNSVEHVKKYWSCLNFHPTTKQIQSTKWQDRNPWSSAFISWCMKAAGY
                     GNNFFYAPNHAKYIRKALQNRENNLTENPFWAYDITEEEAAYPKPGDLLCKNREGMKY
                     ILHSIKENSISHCDVVVETDKINGIVITIGGNVSDKVNKRWVFLDNNGFIDTNAAWLS
                     FDADGVGHSGSQKDFFSVIKVQ"
     gene            1784..2737
                     /locus_tag="PLY81_07240"
     CDS             1784..2737
                     /locus_tag="PLY81_07240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNF30058.1"
                     /translation="MKVTEHIAQAKDTLLSFEILPPLKGKTIQTLFNHLDPLMEFNPS
                     WINVTYHRSETAFKKKQDGTFEKVEIRKRPGTVGICAAIMNHYKIDTVPHFICGGFNK
                     RESEDALIDLNFLGINNVLVLRGDAEKNEPSFLPEPSGHQYAIDLQKQVVNLNHGIYL
                     DDDIRDGGKTNFCIGTAGYPEKHFEAPNLEIDLQRLKNKVDAGADYIMTQMFFDNEKF
                     FSYVNQCRNMGVTVPIIPGLKPITSKKQLSVLPRIFHVDIPTELSTEIMKCRTDEDVE
                     TVGTEWLIHQSKALKKFGVPVLHYYTLGKPKVIWNVVKEVI"
     gene            2826..3230
                     /locus_tag="PLY81_07245"
     CDS             2826..3230
                     /locus_tag="PLY81_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30059.1"
                     /translation="MQKIIAYILFFIFSISTFTSKAVIDDCCAVNNAKHTMPSHCKKS
                     CCTKEKKSIPKHAHKCCQVIAPNQLNYAVSNTNNIVVEASVSSKKIHCITYSYSFQNL
                     FEKPNSNYPIFFKQHTLQYAPKHYIQYCSYLI"
     gene            3284..>3897
                     /locus_tag="PLY81_07250"
     CDS             3284..>3897
                     /locus_tag="PLY81_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30060.1"
                     /translation="MKTLLLYTICLLLSLNVNAQTHKTYSFKVKGSCEMCKERIEQAA
                     KTAGAININYNLATQVLSFSLDNEKLNVADIEKAVANIGHDTEHFSSPDKTYNELPDC
                     CHYTRGNQFNTSKNLLRGIILEENTSGKMMPIAHVNVHDLVNEFSVQTNAKGAFEIPF
                     QSATYLSINHVNFKKDSIEVNENEFLTITLSHKGKNSLDDVVVTV"
BASE COUNT         1392 a          583 c          601 g         1321 t
ORIGIN      
        1 tcagttaaag aaaccctgat ggtatctcca ataccatctt ccaataaagt tccaatacct
       61 gctgcacttt tcactcttcc atcttcgcca tcacctgctt ctgttacacc taaatgcagt
      121 ggataacatt caccaaactc agtttccatt tgttgtataa gtaacctata agcctgtacc
      181 attacttgtg ggttacttgc tttcatgctt agtaaaatat tatggtaaga ttcgtttcga
      241 gcaattctta aaaactccat tgcactttca accataccca ttggtgtatc tccatatcgg
      301 ctcataattc tatcacttaa tgaaccatga ttagtaccaa ttctcattgc agtaccatac
      361 tctctacaaa ttttaattag aggagtaaat cgttctctta ttctctctat ttcctccaaa
      421 tattctgcat cagtatattc aatttgttca aatttttttt tatctacata attgccaggg
      481 tttactctaa ctttttcaac tattctggca gatatttcag cagctttggg tgtaaaatgt
      541 atatctgcca ctaaaggtat tttaaaacca cgtttatgca attcattttt aatgtgtagt
      601 aaattttctg catcttttat acttggagca gtaattctta caagttctgc acctgcttca
      661 atacaacgaa tagtttgctc aactgtagca atagtattca tggtatcggt tgtagtcatt
      721 gtttgaagcc taataggatg attgtttcct aatagcaaat cgccaatttt aacttctttg
      781 gttaataaac gtttatagtt ggttaaagaa ttacaatata gttgcatagc ataataataa
      841 agatgcaaag ttgcttaaaa aatacctatt ttaattaata aattaattgt gcaaaaatta
      901 gatcataatt tataaattat ataagatacc aactaatggg tatttttaaa atggatgctt
      961 gtaaataagt atttgcagca tatattagct aaatgaaatt gctagtttta gtatatatgc
     1021 tggtatggtg tggtacactt gttgctcaaa cagaacgttc ggatagttta ctcaatttta
     1081 aaaacaatct tttgcaaata gtgcaagaag aatattgttt ttggaacgat cctgtaaata
     1141 gagttgaaac acagtttaat tcggttgaac atgttaaaaa atattggagt tgtttaaatt
     1201 ttcatcctac aaccaagcaa attcaaagta caaaatggca agatagaaat ccttggagtt
     1261 ctgcatttat ttcatggtgt atgaaggctg caggctatgg caataatttt ttttatgctc
     1321 ctaaccatgc aaaatatata agaaaagctt tacagaatag agagaataac ttaaccgaaa
     1381 atccattttg ggcttatgat attaccgaag aagaagctgc ttacccaaag ccaggagatt
     1441 tactttgcaa gaatagagaa ggaatgaagt atatcttaca ttcaatcaag gaaaattcta
     1501 tctcacattg tgatgtagtg gttgaaacag ataagattaa tggtattgta attactatag
     1561 gaggcaatgt ttcggataag gtaaacaaac gctgggtttt tttagataat aatggattta
     1621 ttgatactaa tgcagcatgg ttaagttttg atgcagatgg ggtaggccat tctggtagcc
     1681 aaaaagattt tttttcggta attaaagtac aatagttttt tgtaacaata cactttttaa
     1741 agtacccaat acttattatg tttgcagcta tattttttat ttaatgaaag ttacagagca
     1801 tattgcacaa gcaaaagata ccttactgtc ttttgaaata ttacctcctt taaaaggaaa
     1861 aacaattcaa accttattca atcatttaga cccattaatg gagtttaacc caagttggat
     1921 aaatgtaact tatcatagaa gtgaaacagc ttttaagaaa aaacaagatg gcacttttga
     1981 aaaagttgaa ataagaaaaa ggccaggtac agtaggtatt tgtgctgcta taatgaacca
     2041 ttataaaatt gatacagtac cacattttat ttgtggaggt tttaataaaa gagaaagtga
     2101 agatgcatta attgacctta actttttagg cattaacaat gtattggtat taagaggaga
     2161 tgctgaaaaa aatgaaccta gttttttacc agaaccaagt ggccaccaat atgccattga
     2221 cttacaaaaa caggttgtaa acttaaacca tggtatttat ttagatgatg atattagaga
     2281 tggtggaaaa actaattttt gcattggcac cgcaggatat ccagaaaagc attttgaagc
     2341 tccaaatcta gaaattgact tacaaaggtt aaaaaataaa gtagatgcag gtgcagatta
     2401 tattatgaca caaatgtttt ttgataacga aaagtttttt agttatgtaa atcaatgccg
     2461 aaatatgggt gttactgtac caattatacc agggttaaaa cctattacat ccaaaaaaca
     2521 attaagtgta ttgccacgta tttttcatgt tgatattcca actgaactat caacagaaat
     2581 tatgaaatgt agaactgatg aagatgtaga aactgttgga acggaatggt taattcacca
     2641 aagtaaggca ttaaaaaaat ttggtgtacc tgtattacat tactacacat taggaaagcc
     2701 aaaagtgata tggaatgttg ttaaagaagt aatttaaaca tattttaatt tttgaatcta
     2761 tctattaatc tcttttttgt tatagattaa cattgtaatt tctcacaaat gcctaatttt
     2821 gcactatgca aaaaattatt gcatacatat tatttttcat ttttagtatt agcactttta
     2881 ccagtaaagc tgtaatagac gattgttgtg ctgttaataa tgctaaacat acaatgccta
     2941 gccattgtaa aaaatcctgc tgtacaaaag aaaaaaaatc aatacctaaa catgcacata
     3001 aatgttgcca ggttattgct cccaatcaat taaactatgc tgttagtaat accaataata
     3061 ttgttgtaga agcttctgtt tcttccaaaa aaattcattg tattacctat tcttacagtt
     3121 ttcaaaattt atttgaaaaa ccaaactcaa actatccaat cttttttaag cagcatactt
     3181 tacagtatgc cccaaaacat tacatacagt attgtagtta tttaatatag atagcttcca
     3241 ctttcttttt ttaaattatt ttttaaattt taaaattcat taagtgaaaa ctttattatt
     3301 atatactata tgcttattac taagcttaaa cgtaaatgca caaacacata aaacctattc
     3361 ttttaaagta aaaggtagct gcgaaatgtg taaagaaaga attgaacaag cagcaaaaac
     3421 agcaggggca attaacatta actataattt agccacacag gttttaagtt ttagtttaga
     3481 taatgaaaaa ttaaatgttg cagatattga aaaagcagta gctaatattg gccacgatac
     3541 agaacatttt tcctctccag ataaaacgta taacgaatta ccagattgtt gtcattatac
     3601 cagaggtaat cagtttaata cttccaaaaa cttattaaga ggtattattt tagaagaaaa
     3661 taccagtggt aaaatgatgc caattgcaca tgtaaatgtg catgatttgg ttaatgaatt
     3721 ttcggtacaa accaatgcaa aaggagcttt cgaaattcct ttccaatctg ccacatattt
     3781 atctataaac catgtaaact ttaagaaaga tagtattgaa gttaatgaga acgaattttt
     3841 aactataacc ttaagccata aaggcaaaaa cagtttagat gatgtggtag ttactgt
//
LOCUS       DAOQHP010000211         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14351_length_3886_cov_4.016706, whole genome shotgun sequence.
ACCESSION   DAOQHP010000211 DAOQHP010000000
VERSION     DAOQHP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14351_length_3886_cov_4.016706"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2348
                     /gene="leuS"
                     /locus_tag="PLY81_07255"
     CDS             <1..2348
                     /gene="leuS"
                     /locus_tag="PLY81_07255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNF30061.1"
                     /translation="TDLIEKYATKGTKHFSSNQWNAFTDAEKEVWLMKRRLAFSSYGE
                     VNWCEALGTVLANDEVVNGVSERGGHPVIKKKMRQWYLRITAYADRLLQGLETVDFSD
                     SMKEMQRNWIGKSEGAEINFKISKPFKDDTNNNKEFSDVIKVYTTRPDTIFGVDFMVI
                     APEHEMVETIISEEQKLYVEKYIAYVKSRSDRERQAEKNISGVFTGSYAINPFDGKKI
                     PIWISEYVLIGYGTGAIMAVPCGDERDFKFAKHFNIPITNIIGNHFNGLEANPTKEAT
                     LENSNFLNGLVMKDAITIAINKLEELGIGKRKVNYKMRDAGFSRQRYWGEPFPIIWKE
                     GTPTALSASQLPLELPHVEKYSPGPEGEGPLANIQNWINTPEGKRESSTMPGYAGSSW
                     YFLRYMDPENNQEFVSRTASDYWNSVDLYIGGTEHAVGHLLYSRMWTKVLYDLGYIGF
                     DEPFKRLVNQGMIQGSSRFVYRINGTNTYVSKNLISEGDQYLYQEKKVDKMHVDVNMV
                     DGLELNIEQFKQWRNGENNNAEFILEGEKYICGVEVEKMSKSKYNTVNPDDLCVQYGA
                     DTFRMYEMFLGPVEQSKPWDTKGIEGVHRFLKKLWRLFYSESSFSNGIQTNGNTIFTK
                     DAATSEELKVLHKTIKKIDEDTERFSYNTAVSAFMVCVNELADLKCFKKEILENVLIL
                     LAPYAPHIAEELWNVLGNEGSVLDAAYPIYNSNYTIESSKEYPVSINGKLRTTMHFDI
                     NAEQKDVEPVVLANEIVQKWLEGKNPKKIIFVKNKMINIVV"
     gene            2374..3204
                     /locus_tag="PLY81_07260"
     CDS             2374..3204
                     /locus_tag="PLY81_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF30062.1"
                     /translation="MFENVITYFSTLEQRPLERLAFLVVGLLFFWIMEGYIPLITMQY
                     KKNKLKHAGVNFAFTVIHLIIHTFLALAIIKISDWCLANNFGLVYWIKTNVLFTILIS
                     FLILDFFGGWLVHIVEHKIPLLWRFHVIHHSDNNVDVTTGLRHHPIESVIRGIFFFAG
                     IVVSGAPMYAVMIFQTILVISTAFTHANIALPLWLDKLLSYFIVSPNMHKVHHHYKQP
                     FTDSNYGAILSIWDRLLGTFSHLHHSKIKYGLDNFYPNEKDEDFMLLMKKPFGKMTKE
                     "
     gene            3236..3826
                     /locus_tag="PLY81_07265"
     CDS             3236..3826
                     /locus_tag="PLY81_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HNF30063.1"
                     /translation="MIYQFKNYIPVVHQSSFVHPQAAVTGNVIIGKNVYVAPGAAIRG
                     DWGKIIIEDGCNIQENCTIHMFPGITVVLKEAAHIGHGAIIHGATIGKNCLIGMNAVI
                     MDNVVLGNECIVGALSFIKADEKFEDRSLIVGNPAKKIKEVSNEMIAWKTEGTKLYQQ
                     LPTELHAYLKAVEPLREMPSNSNNEESVYKTWNETK"
BASE COUNT         1396 a          511 c          738 g         1241 t
ORIGIN      
        1 ttaccgattt gattgaaaag tatgccacta aaggcaccaa acatttttct tctaatcaat
       61 ggaatgcttt tacagatgca gaaaaagaag tgtggttaat gaaaagaaga ttagcatttt
      121 cttcttatgg agaggtaaac tggtgcgaag ctttaggaac tgttttagcc aatgacgaag
      181 ttgtgaatgg tgtaagtgaa agaggagggc accctgttat aaaaaagaaa atgaggcagt
      241 ggtacttaag aattacagcc tatgcagata gacttttaca aggtttagaa actgttgact
      301 ttagtgatag catgaaagaa atgcaacgta actggattgg taaaagtgaa ggagcagaaa
      361 ttaattttaa aattagtaaa ccctttaaag atgatactaa taacaataaa gaatttagcg
      421 atgttataaa ggtttacact acacgacctg atactatttt tggtgttgac tttatggtta
      481 tagccccaga gcatgaaatg gttgaaacta ttatttctga agaacaaaaa ctatatgttg
      541 aaaaatatat agcttatgta aaaagtagaa gtgatagaga acgccaagca gagaaaaata
      601 tatcaggggt ttttactggt tcttatgcta taaatccttt tgatggtaaa aaaattccta
      661 tttggataag tgaatatgtg ttgataggtt atggcactgg tgcaattatg gctgtacctt
      721 gtggcgatga acgagatttt aagtttgcaa aacactttaa tattcctatt actaatatta
      781 ttggaaacca ttttaatgga ttagaggcta accctactaa agaggccact ttagaaaaca
      841 gtaatttttt aaatggctta gtaatgaaag atgctattac cattgctatt aataaactag
      901 aagaattggg cattggtaaa cgaaaggtaa attataaaat gagagatgct ggctttagta
      961 ggcaacgcta ctggggcgaa ccttttccta ttatttggaa agaaggaaca cccacagcat
     1021 taagtgccag ccaactacca ttagagctac ctcatgtaga aaagtatagc ccaggccctg
     1081 aaggtgaagg accactagca aatattcaaa attggataaa tacacccgag ggtaaaagag
     1141 aatcctcaac aatgcctggt tatgctggtt catcttggta ctttttacgc tatatggacc
     1201 ctgagaacaa ccaagaattt gtaagtagaa ctgcaagtga ttattggaat agcgttgact
     1261 tatatattgg tggtacagag catgctgttg ggcatttatt atatagccgt atgtggacta
     1321 aagtattgta cgacttaggc tatattggtt ttgatgagcc atttaaaagg ttagtaaatc
     1381 aaggaatgat acaaggtagc agcaggtttg tatatagaat aaatggaacc aatacctatg
     1441 tttctaaaaa cttaattagc gaaggtgacc aatatttgta tcaagaaaaa aaggtggata
     1501 aaatgcatgt agatgtaaat atggttgatg ggcttgaatt aaatattgaa caatttaaac
     1561 aatggcgtaa tggtgaaaat aataatgctg aatttatttt agaaggtgaa aaatatatct
     1621 gtggagttga agtagaaaaa atgtcaaagt ctaaatacaa tactgttaac cccgatgatt
     1681 tatgtgtaca atatggtgcc gatactttta gaatgtacga aatgttttta ggccctgtag
     1741 aacaaagcaa accatgggac actaaaggta ttgaaggcgt tcatcgcttc ttaaaaaaat
     1801 tatggcgttt attttatagc gaatctagtt tctcaaacgg tattcaaact aacggaaata
     1861 ctatttttac aaaggatgct gctacttctg aagaactaaa agtgttgcat aaaaccatta
     1921 aaaaaataga tgaagataca gaacgttttt cttacaatac agctgttagt gcttttatgg
     1981 tttgtgtaaa cgaattggca gatttaaagt gctttaaaaa agagatttta gaaaatgtat
     2041 taattctatt agcaccttat gcaccacata ttgctgaaga actatggaat gttttaggca
     2101 acgaaggaag tgtattagat gctgcctatc ctatttacaa tagcaactac actattgaaa
     2161 gtagtaaaga atatcctgta agcattaatg gaaaattaag aacaacaatg cattttgata
     2221 ttaatgcaga acaaaaagat gtagaacccg ttgtgcttgc aaatgaaatt gtacagaaat
     2281 ggttagaagg aaaaaatcct aaaaaaataa tttttgtaaa aaataaaatg ataaacatag
     2341 tagtttaatt tacacaacta taatacacat tatatgtttg aaaatgtaat tacttatttt
     2401 tctaccttag agcaacgccc tttagaaagg ttggcatttt tagtagtggg gttgttattc
     2461 ttttggataa tggaaggata tataccttta attaccatgc agtataagaa aaataagctt
     2521 aagcatgctg gcgtaaattt tgcatttact gttatacatt taataataca tactttttta
     2581 gctttagcaa ttattaaaat aagcgattgg tgtttagcca acaattttgg tttagtgtat
     2641 tggattaaaa ccaatgtatt atttactata ttaatttcat ttttaattct cgattttttt
     2701 ggtggttggc tggtacatat tgtagaacat aaaatacctt tattgtggcg ttttcatgta
     2761 atacatcatt cagataataa tgtagatgta actacaggtt tacggcatca tcctatagaa
     2821 agtgtaatac gtggcatttt cttttttgca ggcattgtag taagtggtgc acctatgtat
     2881 gctgtaatga tttttcaaac cattttagtt atttctaccg catttactca tgccaatatt
     2941 gcattgcctt tatggttaga taaattatta agttatttta ttgtatcgcc aaatatgcac
     3001 aaagtacacc atcattacaa acaacctttt accgatagta attatggtgc aatattaagt
     3061 atttgggata ggctgttggg tactttttct catttacacc attctaaaat aaaatatggt
     3121 ttagataatt tttaccccaa tgaaaaagac gaagacttta tgctattaat gaaaaagcct
     3181 tttggtaaaa tgactaaaga ataaatatat tttttagctt ctaaaaattt attcaatgat
     3241 ttatcagttc aaaaattata tacccgttgt gcaccaaagc tcttttgtac acccacaagc
     3301 agcagttaca ggcaatgtaa ttattggtaa aaatgtttat gtagcacctg gtgcagccat
     3361 acgaggcgat tggggtaaaa taattattga agatggttgc aatattcaag aaaactgtac
     3421 tatacacatg tttcctggta ttacagttgt tttaaaagag gctgctcata ttggtcatgg
     3481 agctattata catggagcca ctattggtaa aaactgctta ataggtatga atgcggtaat
     3541 aatggataat gttgttttag gaaatgaatg tattgtaggt gctttaagtt ttataaaagc
     3601 cgatgaaaaa tttgaggata gaagtttaat agttggcaat cctgcaaaaa aaataaaaga
     3661 agtaagtaac gaaatgatag cttggaaaac tgaaggaact aaactgtatc aacaattacc
     3721 aacagaattg catgcttatt taaaagcagt ggaaccttta agagaaatgc cttctaatag
     3781 caacaacgaa gagagtgttt acaaaacatg gaatgaaact aaataaattg agacactatc
     3841 ccataaagtg tgtaagtaaa ataattaggg gttcagcttt tttata
//
LOCUS       DAOQHP010000212         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14520_length_3857_cov_4.311152, whole genome shotgun sequence.
ACCESSION   DAOQHP010000212 DAOQHP010000000
VERSION     DAOQHP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_3857_cov_4.311152"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLY81_07270"
     CDS             <1..196
                     /locus_tag="PLY81_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNF30064.1"
                     /translation="KNAIKQDNLTWTHVSDLKFWDNAAARLYNVNSIPYNLLIDPNGK
                     IVAKNLRAEELHETLEKILK"
     gene            292..1275
                     /locus_tag="PLY81_07275"
     CDS             292..1275
                     /locus_tag="PLY81_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF30065.1"
                     /translation="MSFLKVENLSKVINNKAIVNQVSFKQFKRENIAIAGATGSGKTT
                     LLKLIAGLLQPTNGNVFLEENRVKGSEEKLLPGHKSIAYLNQHFELRNNYFVHELLDV
                     FNAITEEEAYRIYTICAITNLLQRKTNELSGGEKQRIALAAQLVTNPSLLLLDEPFSN
                     LDLWQKNNIKNVLTDLQKELQLSIILTSHDWTDILPWANTILVMKNGEVIQQGNPFTI
                     YHNPINEYVAGLSGAYNIIDVSNVQHFSNSILKQDALKKIFLRPEQIEIEPANNIFQK
                     DVIQEVYFYGSYNIVDVLVNNEIVKVKTTNNTLTVGQRVFLTVKPIEFWYL"
     gene            1350..1475
                     /locus_tag="PLY81_07280"
     CDS             1350..1475
                     /locus_tag="PLY81_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30066.1"
                     /translation="MFGKILINKQAIIFKNVNTIIIDEIGRLNSGTFNVQIMMNN"
     gene            complement(1615..2343)
                     /locus_tag="PLY81_07285"
     CDS             complement(1615..2343)
                     /locus_tag="PLY81_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30067.1"
                     /translation="MKSKLLIFAMYSITLALVFSSCKKDSGDTQYVCTTCNSTPEGLA
                     ANDNLAKGVYKGVVIGSSGTIKFDINNSGNTITATLTLDGQTATLTSQVSVPPSGTYM
                     SPFTGTLNGQPVSVTFSVNFDGTSPTITTSNIPGHPNAVFIIAKETSTVLVRCFEGTY
                     STNEPENGTFNIVVSTILKGWKGKSRETGSTTTKDIDGSYVNNILYFGTGTTTPIATI
                     NGDSFSGSFFDGSYTVTVNGQRKS"
     gene            2527..3171
                     /locus_tag="PLY81_07290"
     CDS             2527..3171
                     /locus_tag="PLY81_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30068.1"
                     /translation="MKTYKVPILLTIIMFVLLNNIQSQKAEIYYGKINTIMGNFEGQF
                     KINLNDENKDMIEIKIAVIKQKKKGEKIIYKEVLKVNAAGIRSFEIDSTIYIVKNIEL
                     ENNKTLQNCCLRITDSCSVATLAYWGTSLENDKCFVLTKNSSYYKTLKSISYFIYFTN
                     FSKCETIKNKNIEEENKWNSDGLNRMKAYEALTQQELLERWKSRITAYKNCFSN"
BASE COUNT         1497 a          497 c          649 g         1214 t
ORIGIN      
        1 gaaaaatgca attaagcaag acaacttaac ttggacacat gttagtgatt taaaattttg
       61 ggataatgct gctgcaagat tgtataatgt taacagcatt ccatataatt tactaattga
      121 ccctaatggt aaaattgtgg ctaaaaactt aagagccgaa gaactgcatg aaaccttaga
      181 aaaaatatta aaataacttt tggttatagt aatagaaaat gctgtctgtt ttttggcagc
      241 atttttttta ttaaataaaa gctacattat aagtaagttt atttttgatt tatgagtttt
      301 ttaaaagtag aaaatttaag taaggtaata aataataaag caattgtaaa ccaagttagt
      361 tttaagcaat ttaaaagaga aaatattgca attgcaggag ctacaggctc ggggaaaaca
      421 accttattaa aattaattgc tggtttatta caacctacca acggaaatgt gtttttggaa
      481 gagaataggg taaaaggctc agaggaaaaa ttattaccag ggcataaaag cattgcatat
      541 ttaaaccaac attttgagtt aagaaataat tattttgtac atgaactgtt agatgttttt
      601 aatgcaataa cagaagagga ggcttataga atttatacta tttgtgcaat taccaattta
      661 ttacaaagaa aaaccaatga actatctggt ggagaaaaac aacgaattgc cttagctgca
      721 caactggtta caaacccatc attgctattg ttagatgagc ctttttctaa tttagattta
      781 tggcaaaaaa ataatataaa aaacgtttta acagaccttc aaaaagaatt acaattaagt
      841 attatcctaa catcgcatga ttggacagat attttgcctt gggcaaacac aattttggta
      901 atgaagaatg gagaagtaat acaacaggga aatcctttta ctatttatca caatccaatt
      961 aatgaatatg tggcaggatt aagtggagca tataatatta ttgacgtatc caatgttcag
     1021 cattttagta attcaattct taaacaagat gctttaaaga aaatattttt acgaccagaa
     1081 caaatagaaa tagaacctgc taataatatt tttcaaaaag atgtaattca ggaagtttat
     1141 ttttatggaa gttacaacat tgtagatgtt ttagtaaata atgaaattgt aaaagttaaa
     1201 acaactaata atactttaac tgttgggcaa agggtatttt taacagtaaa gccaattgaa
     1261 ttttggtatt tatgatgtag aatgtaaaat gaaattttat tttattacaa ctgaaaaaag
     1321 aggaagcagt tttcattaga gtaatagata tgtttggtaa aatattaata aataaacaag
     1381 ccataatttt taaaaatgta aatacaatta ttatagacga aattggtaga ttaaactctg
     1441 gtacatttaa tgtacaaata atgatgaata attaaatgtt aactgagaag ttgattgtaa
     1501 caaaataata atccctatgt tttcgaaaaa gagagaggct gcacaaatgc agcctctctc
     1561 ttttgaataa taaagaacat aaatattagg aaaaagtaaa aaggaagtaa ctaattacga
     1621 ttttctttgc ccatttactg ttacagtata gcttccatca aaaaaagaac ctgaaaaaga
     1681 atctccgtta atagttgcta ttggagtagt tgtgccagta ccaaaataca aaatattgtt
     1741 tacatagcta ccatcaatat ctttggtagt ggtactacca gtttctcttg attttccttt
     1801 ccagcctttt aaaatggtag atactactat attaaaagtt ccattctctg gttcattggt
     1861 tgaataagtt ccttcaaagc atcttaccaa aactgttgaa gtttcttttg caattataaa
     1921 aactgcatta ggatgtcctg gaatattgga tgtagtaatg gtgggtgatg tgccatcaaa
     1981 gtttacagaa aaagtaaccg aaacaggttg tccatttaat gtaccagtaa atggagacat
     2041 ataagttcca gatggtggta cagatacttg agaagttaaa gtagcagttt gcccatctaa
     2101 tgttaaagta gcggtaatag tgtttcccga attgtttata tcaaacttta tggtaccact
     2161 cgaccctata acaactcctt tataaacacc ttttgcaaga ttatcgtttg ctgccaatcc
     2221 ttctggtgta ctgttgcatg tagtacatac atattgtgta tcaccactat cttttttaca
     2281 agatgaaaac acaagtgcca atgtaattga atacattgca aaaatcaaaa gttttgattt
     2341 cataaatgtg gttttaattg tttttaatta gaatatagca actactacat tctaaaagta
     2401 aatctatgta ctaatcttga aatccttaat acccaccaga aggtatttta aaaattatca
     2461 ataacctctt aaaataaaat agatattttt actaacaatc ataaacactt tgttatataa
     2521 aaaatcatga aaacgtataa agtaccaata cttcttacaa ttattatgtt tgttttatta
     2581 aacaatattc aatctcaaaa agcagagatt tattatggaa aaataaacac aatcatgggc
     2641 aattttgaag gacaatttaa aattaatttg aacgatgaaa ataaggatat gattgaaata
     2701 aaaattgccg ttattaaaca aaagaaaaaa ggcgaaaaaa ttatttacaa agaagtatta
     2761 aaagtaaatg cagcaggaat tagaagtttt gaaatagata gcacaattta tattgtaaaa
     2821 aatatagagt tggagaataa caaaacgctt caaaattgtt gtttaagaat aacagatagt
     2881 tgtagtgtag ctactttagc ttactgggga acaagtttag aaaatgataa gtgttttgta
     2941 ttaactaaaa atagtagtta ttataaaact ttgaaatcaa taagttattt tatttatttc
     3001 accaatttta gtaaatgtga aactataaaa aataaaaaca ttgaagaaga gaataaatgg
     3061 aattctgatg gcttgaatag aatgaaagct tatgaagcat tgacacaaca agaattattg
     3121 gaaaggtgga aaagtagaat aacagcgtat aaaaattgtt ttagtaatta gtaaactatt
     3181 tgtttaaaat ttgctgtact gttactaata aaattaaaca aaaaaggttt gtcattctga
     3241 atgaaatgaa gaatctgaat gtactttgga acaaaccact agtagatcct tcgctacgct
     3301 caggatgaca aaaatgggat ggttcagaat gacaatgcac acttactgtc atgttgaggc
     3361 aacgaagcat ctgtaaagtt aaggatagag attcctcgca aactcggaat gacatagaat
     3421 taaagatcct tcgcttcgct caggatgaca atctatgagt gctcaggatg acataaatgg
     3481 gagagttctg aatgacaatg tacacttact gtcatgctga ggcaacgaag catctgtaaa
     3541 gttaaggata gagattcctc gcaaacacgg aatgacatag aattaaagat ccttcgctac
     3601 gctcaggatg acaaacgttg agtgctcagg atgaaatgat ttaataaatt gaaacaaaaa
     3661 agggtttgtc attctgaatg aaatgaagaa tctgcaagtg cttttgcaca aaccattagt
     3721 agatccttcg ctacgctcag gatgacaaac tatgagtact caggatgaca taaatgggag
     3781 agttcaggat gatgcggttt attgtaaaaa tgacactatc ccataaagtg tgtaagtaaa
     3841 ataattaggg gttcagc
//
LOCUS       DAOQHP010000213         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14524_length_3857_cov_2.684903, whole genome shotgun sequence.
ACCESSION   DAOQHP010000213 DAOQHP010000000
VERSION     DAOQHP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14524_length_3857_cov_2.684903"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2277
                     /locus_tag="PLY81_07295"
     CDS             <1..2277
                     /locus_tag="PLY81_07295"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNF30069.1"
                     /translation="FLFMAFDIEMIKKAYAEMPEKINAARTKMGRSLTLSEKILFAHL
                     HSDMKLENFERGKSYVDFAPDRVAMQDATAQMALLQFMQAGRSKVAVPSTVHCDHLIM
                     AKLNAKNDLEIANQESKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMI
                     GTDSHTVNAGGLGMIAIGVGGADACDVMAGLAWELKWPKLIGIKLTGKLSGWAAPKDV
                     ILKVAGLLTVKGGTGAIVEYFGEGATAMSCTGKGTICNMGAEIGATTSTFGYDESMSR
                     YLKSTGRADVAALADNIKEHLTADAEVYTNPEKYFDQVIEINLSELEPHLNGPFTPDL
                     ATPISKMKEEAAKNGWPTKVEVGLIGSCTNSSYEDISRAVSLAKQVSEKGLKTKADYT
                     ITPGSEQVRYTIQRDGFLEVFDKIGATVFANACGPCIGMWDRVGAEKKEKNTIVHSFN
                     RNFAKRADGNPNTFAFVASPEIVTALAIAGDLTFNPITDTLVNEKGELVKLDEPTGDE
                     LPSKGFAVEDAGYQAPAADGSNVVVNVSPTSNRLQLLDPFAAWDGNDLKNLKLLIKAK
                     GKCTTDHISMAGPWLKFRGHLDNISNNLLIGAINFFNDKADNVKNQLTGEYGTVPATQ
                     RAYKAAGIGSIVVGDENYGEGSSREHAAMEPRHLGVKVVLTKSFARIHETNLKKQGML
                     ALTFANKEDYDKILEDDTIDVIGLTTFSPNDALTLVLHHKNGIKEEIVANHTYNQQQI
                     EWFKAGAALNIIRKQVAY"
     gene            complement(2355..2636)
                     /locus_tag="PLY81_07300"
     CDS             complement(2355..2636)
                     /locus_tag="PLY81_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30070.1"
                     /translation="MDFIVETLKNLIPYFLLSIVFVFVLKSFLVTKVKSFDLAEIFFS
                     FFRLYNYDEINMSSNKRRVTFMRWNNLFNYYTYFVIGLVILVYLVTKDA"
     gene            2745..>3857
                     /gene="lepA"
                     /locus_tag="PLY81_07305"
     CDS             2745..>3857
                     /gene="lepA"
                     /locus_tag="PLY81_07305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNF30071.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLADRLLEHTKTINERKMQAQVLDDM
                     DLEREKGITIKSHAIQINYTYNNQQYVLNLIDTPGHVDFSYEVSRALAACEGALLLVD
                     ASQGIQAQTISNLYLALDNDLEIIPVINKIDMEGAMVLEVTDQIVDLIGCKPEEILLA
                     SGKSGIGIEEILQAIVHRIPSPKGNTEAPLQALIFDSVFNSFRGIIIYYRILNGCIKK
                     GDKIRFCATGEDYEAMEVGILKFGMEEKKEINCGDVGYLITGIKNAKEVKVGDTITLA
                     NNPADAIKGFEEVKPMVFAGIFPIDATDFENLKEALEKLSLNDSSLTFETEKSAALGF
                     GFRCGFLGLLHMEIVKAPRIHIENQNQGQQIFDVDSW"
BASE COUNT         1418 a          534 c          741 g         1164 t
ORIGIN      
        1 tttttattta tggcttttga tattgaaatg attaaaaagg cttatgcaga aatgccggaa
       61 aaaataaatg ctgctagaac taagatgggg cgttctctta cattatccga aaaaatattg
      121 tttgctcact tacatagcga catgaaattg gaaaattttg aaagaggaaa aagctatgta
      181 gattttgcac cagaccgtgt agcgatgcaa gatgcaactg cacaaatggc attattacag
      241 tttatgcaag ctggtcgttc aaaagttgca gtgccaagta cagtacattg cgaccatcta
      301 ataatggcta aattaaatgc aaaaaacgat ttagaaattg ctaatcaaga aagcaaagaa
      361 gtgtatgatt ttcttgcttc agtatctaat aaatatggaa ttggattttg gaaaccaggt
      421 gcaggaatta tacatcaggt tgttttagaa aattatgcat ttcctggagg tatgatgata
      481 ggaacagata gccatactgt taatgcaggt ggcttaggca tgattgcaat aggcgttggt
      541 ggtgcagatg cttgtgatgt aatggctggc ttagcttggg agcttaaatg gccaaagcta
      601 attggtataa aactaacagg taaacttagt ggttgggcag ctcctaaaga tgttatttta
      661 aaagtagcag gtttgttaac tgtaaaaggt ggcacaggag ctattgtgga atattttggt
      721 gaaggtgcta cagcaatgag ttgtacaggt aaaggaacta tttgtaatat gggagcagaa
      781 attggtgcaa ccacttctac atttggttat gatgaaagca tgagcaggta tttaaaatct
      841 acaggaagag ctgatgtagc tgcattagca gataatatta aagaacactt aactgccgat
      901 gcagaagttt atactaatcc tgaaaaatat tttgaccaag ttattgaaat aaatttaagc
      961 gaacttgagc ctcatttaaa tgggccattc acgccagatt tagcaactcc aatttctaaa
     1021 atgaaagaag aagctgcaaa aaatggttgg ccaactaaag tagaagttgg cttaattggt
     1081 agctgtacta atagttctta cgaagatatt tcaagagctg taagtttagc caaacaagta
     1141 tctgaaaaag ggttaaagac aaaggcagat tatactatta caccaggcag tgagcaagtt
     1201 agatatacta ttcaaagaga tggtttttta gaagtatttg ataaaattgg agccaccgtt
     1261 tttgcaaatg cttgtgggcc ttgtattggt atgtgggata gagtaggagc agaaaagaaa
     1321 gaaaaaaata caattgttca tagctttaat agaaattttg ccaaacgagc cgatggtaat
     1381 ccaaatactt ttgcttttgt agcaagcccc gaaattgtaa ctgcattagc tattgcagga
     1441 gatttaactt ttaatccaat aaccgatact ttagttaatg aaaaaggaga acttgtaaaa
     1501 ttagatgaac ctacaggcga tgaattacca tctaaaggct ttgcagttga ggacgcaggc
     1561 tatcaagcac ctgctgcaga tggtagtaat gttgttgtaa atgtttcgcc aactagcaat
     1621 cgtttacaac tattagatcc ttttgctgct tgggatggta atgatttaaa gaatttaaaa
     1681 cttttaataa aagcaaaagg gaaatgtact accgaccata tttctatggc tggcccatgg
     1741 ttaaaattta gaggccattt agataatatc tctaacaatt tattaatagg tgcaattaac
     1801 ttttttaatg ataaagcaga caatgtaaaa aatcaattaa caggagaata tggtactgta
     1861 cctgctacac aacgtgccta caaagctgca ggcattggaa gcattgtagt tggagatgaa
     1921 aactatggag aaggctcttc tagagaacat gctgcaatgg agcctcgtca tttaggagta
     1981 aaagttgttc taacaaaatc ttttgctcgt attcatgaaa ctaatcttaa aaagcagggt
     2041 atgttggctt taacttttgc taataaagaa gattatgata aaatattaga agatgataca
     2101 attgatgtaa taggcttaac cactttttcg cccaatgatg ctttaacatt ggttttacac
     2161 cataaaaatg gcataaaaga agaaattgta gcaaaccata cttataacca acaacaaata
     2221 gagtggttta aagctggtgc tgctttaaat attattcgta agcaagttgc ttattaaata
     2281 aatattttag gcaaacaaaa atcccatgtt acatagtaac atgggatttt tttatacaac
     2341 gatttataca aattttaagc atctttagta accaaataaa ctaaaataac caatccaata
     2401 acaaaataag tatagtaatt aaaaaggtta ttccaacgca taaatgttac tcttctttta
     2461 ttgctgctca tattaatttc atcgtaatta tacaagcgaa aaaagctgaa aaatatttca
     2521 gctaaatcaa aacttttaac cttagttacc aaaaagcttt ttaaaacaaa aacaaaaaca
     2581 atactcaata aaaaataagg tatcaaattt tttaatgttt ctacaataaa atccattagt
     2641 tttttaaata atttataata ataataaggg tataaataaa aattgagcaa ataataaaac
     2701 aaatataagt aaaaaaattt tgtattgtta tctttgccgc aattatgaag catatacgga
     2761 atttttgcat tattgcacac atagatcatg gtaaaagcac tttagcagat agattgttag
     2821 aacacactaa aaccattaac gaaagaaaaa tgcaggctca ggttttagat gatatggact
     2881 tagaaagaga aaaaggaatt actattaaaa gccatgcaat tcaaattaat tatacttaca
     2941 ataatcaaca atatgtatta aacttaattg atactccagg acatgttgat tttagttacg
     3001 aagtaagccg tgcattagca gcatgcgaag gtgctttgtt attggtagat gcaagccaag
     3061 gcattcaagc acaaaccatc agcaatcttt accttgcatt agataatgat ttagaaatta
     3121 ttcctgtaat caataaaata gatatggaag gtgcaatggt tttagaagta accgaccaaa
     3181 ttgttgattt gataggatgt aaaccagaag aaattttatt ggcaagtggt aaaagcggaa
     3241 ttggtattga agaaatttta caagccattg tgcatagaat tccatcgcca aaaggtaaca
     3301 cagaagcacc cttacaagca ttaatttttg atagtgtttt taacagcttt agaggtataa
     3361 ttatttatta tagaatatta aatggttgta ttaaaaaagg agataaaatt agattttgtg
     3421 caacaggaga agactatgag gcaatggaag taggaatttt aaaattcggg atggaagaaa
     3481 aaaaggaaat taattgtggc gatgttggct atttaattac aggcataaaa aatgctaaag
     3541 aagtaaaagt tggcgatact attactttgg caaataaccc tgcagatgca attaaaggct
     3601 ttgaagaagt taaaccaatg gtttttgctg gaatttttcc tattgatgca acagattttg
     3661 aaaacctcaa ggaagcttta gaaaagctgt ctttaaatga ctcaagtttg acttttgaaa
     3721 cagaaaaatc tgctgccctt ggttttggtt ttcgatgtgg attcttgggg cttttgcata
     3781 tggaaattgt aaaagcccca agaatccaca tcgaaaacca aaaccaaggg cagcagattt
     3841 tcgatgtgga ttcttgg
//
LOCUS       DAOQHP010000214         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14645_length_3837_cov_2.984929, whole genome shotgun sequence.
ACCESSION   DAOQHP010000214 DAOQHP010000000
VERSION     DAOQHP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14645_length_3837_cov_2.984929"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLY81_07310"
     CDS             complement(<1..884)
                     /locus_tag="PLY81_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF30072.1"
                     /translation="MHLQLSEEHLMIQKAARDFAVNECLPGVIERDEHQRFPKEQITQ
                     LAELGFMGMMVKPEYGGSGMDTISYVLAMEEVSKIDASVSVCMSVNNSLVCYGLQAYG
                     NEEQKQKYLTPIAQGKKDGELYIGAFLLSEPEAGSDATSQRTIAEDKGDYYLLNGTKN
                     WITNGSSASVYLVMAQTDVSKGSKGINCFIVEKKWAGVTVAAKENKLGIRGSDTHTIM
                     FTDVKVPKENRIGADGFGFTFAMKTLAGGRIGIASQALGIASGAYELALKYAQERKAF
                     GKEIAKHQAIQFKLADMAT"
     gene            1051..2550
                     /locus_tag="PLY81_07315"
     CDS             1051..2550
                     /locus_tag="PLY81_07315"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNF30073.1"
                     /translation="MGIVRKQSIASSIYIYLGFAIGAFNVLFLFPKYLTPAEFGLTRV
                     LLDVSLLIAMFCTLGSCPAVLKFYPFYHSYLPKKKIEIPFFTILLTILGCIIFILIAD
                     GSKSYIVRKFGEKSPLFNEYYYLIYPLSISYAFWYLFEASSWSIKKTVLPNFLKEVGF
                     RIITTILVALYIVKLISFKQFISLFSLMYIVPVIILLYALLKDNFFVITFNISSVTKR
                     LWKQITLFSLFVFTGQTLNVIARTSDTIILASQSKNGLADAAVFTVVTYLVTLMDVPM
                     RGMTGIASSVIAYAWKDKDMNKIKEMYQKTALNLLIVGIGIFGILTINMFNFTQHFGT
                     NYSLLPTLLLILGFAKLIDLATGMNAQILLSSKYWRIDFITSMGFVLISVPLNIFLIK
                     KYGLIGSAYANLISVFIYNTTRLLIIWKLFKLQPYNHKNLLTMVIAAVCFFIVYISPS
                     LNNIYADAILKTVVFMPLYIFLILKLKISEDFNQLLLSFIEKIKIRTKK"
     gene            2550..3254
                     /locus_tag="PLY81_07320"
     CDS             2550..3254
                     /locus_tag="PLY81_07320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30074.1"
                     /translation="MKNTIISFIPPILIELGKKTGILKDKSKVIIYKKNENDTQDLDM
                     YFDPEYAKILDSWGEKTTWIEIQMLLANCKGKVLDIACGSGVTMRILNKYKDLDVHGC
                     DISDLLIKKAVSLGIAENNLKVTDATKMDYADLAFDYSYSIGSLEHFTEKGISEFIAE
                     AARVTKYTSMHMMPTSKSGTNEGWMSTSTTVQTFHNNSDEWWMNKYKQSFSEVIMLNS
                     RWDDGISYGRWFICKK"
     gene            3259..3813
                     /locus_tag="PLY81_07325"
     CDS             3259..3813
                     /locus_tag="PLY81_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF30075.1"
                     /translation="MILRKIIKYLTKTKIPANVIVGKDSVVIGPVSIHTTSSITIGND
                     CLIEGIVTTHTPQAKIEIGNEVFIGNNSFLGCADTIIIEDKVLVSFDCVIQDNDSHST
                     ISSERFTDTKDWKNGRQHNWDLTHKKPIHIKKLAWIGARAIILKGVTIGEGAIVAAGS
                     VVTKDVAPYTIVGGNPAKFIKETT"
BASE COUNT         1264 a          596 c          592 g         1385 t
ORIGIN      
        1 gtagccatat ctgctaactt aaattgtata gcttggtgtt tagcaatttc tttaccaaat
       61 gctttacgct cctgagcata ttttaaagca agctcataag caccacttgc aatacctaat
      121 gcctgagatg caataccaat acgacctcct gctaatgttt tcatagcaaa tgtaaaacca
      181 aaaccatcag cacctattct gttttctttg ggtactttta catctgtaaa cataatagta
      241 tgtgtatcgc taccacgaat gccaagttta ttttcttttg cagcaacagt aacaccagcc
      301 catttttttt caacaataaa gcagttaata cctttgctgc ctttgcttac atcggtttgt
      361 gccataacca aataaacact tgcactgcta ccattggtaa tccagttttt agtaccattt
      421 agtaaataat aatctccttt atcttctgct attgttcgtt ggcttgttgc atcacttcct
      481 gcctctggtt cacttaataa aaatgcacct atataaagtt ctccatcttt tttaccttgg
      541 gctattggtg ttaagtattt ttgtttttgt tcttcgttgc cataagcttg caagccataa
      601 cataccaagc tattatttac actcatacat acactaacac tagcatcaat tttactcact
      661 tcttccatgg ctaatacata gcttatggta tccattccac taccaccata ttctggctta
      721 accatcatac ccataaagcc taattcagct aattgtgtaa tttgttcttt aggaaagcgt
      781 tgatgttcat ctctttcaat tacaccaggt aaacattcat ttactgcaaa atcacgtgct
      841 gctttttgta tcattaaatg ttcctcgcta agttgtaaat gcatagtgtt ttgtattttt
      901 agtgtggcaa aattaggcta atagtgctca gtagaaaaat aaaaaatgaa tgttatctgt
      961 tattaccatt gtttatttaa ttaccattgc ataaaataaa agtattagct attttagtaa
     1021 aatttgagta tttagtttta agcatttttc atgggaatag ttagaaaaca atccatagcg
     1081 tcttctatat atatttattt aggctttgca ataggagctt ttaatgtttt atttctattt
     1141 cctaaatact taacaccagc agaatttgga ttaacaagag ttttattaga tgtttcttta
     1201 ttaattgcta tgttttgtac tttaggcagt tgccctgcag ttttaaaatt ttatcctttt
     1261 tatcattctt atttgccaaa aaagaaaatt gaaattcctt tctttactat tttattaacc
     1321 attttggggt gtatcatttt tatacttata gcagatggta gcaaaagcta tattgttaga
     1381 aaatttggcg aaaaatcgcc actgtttaac gaatattatt atcttatata tccattaagt
     1441 atctcttatg cattttggta tttatttgaa gcatcttcct ggagtattaa aaaaacggtt
     1501 cttcctaatt ttttaaaaga agttggcttt agaattatta ctaccatatt ggtagcattg
     1561 tatattgtta aacttatctc ctttaaacag tttatttctt tattttcctt aatgtatata
     1621 gtgccagtta ttattttact ttatgcatta ttaaaagaca atttttttgt aattactttt
     1681 aacattagct ctgttacaaa acgtttatgg aagcagatta ctttgtttag cctttttgtt
     1741 tttacagggc aaacattaaa tgttattgca cgtacttcgg atacaattat cttagccagc
     1801 caaagtaaaa atggtttggc tgatgctgct gtttttactg tggttactta cttagttact
     1861 ttaatggatg taccaatgag aggaatgaca ggcattgcat catcggttat tgcctatgcc
     1921 tggaaggata aagacatgaa taagattaaa gaaatgtacc agaaaactgc acttaactta
     1981 ttaattgtag gtataggaat ttttggaatt ttaaccataa acatgttcaa ctttacacaa
     2041 cattttggta caaactatag tttattacct acccttttgt taatacttgg ctttgccaaa
     2101 ttaattgact tagctactgg catgaatgca caaatattat tatctagtaa atattggaga
     2161 atagacttta taaccagtat ggggtttgta ttgatctcgg tgccattgaa catattttta
     2221 ataaaaaagt atggtttaat aggttctgcc tatgcaaatt taatttcggt gtttatatat
     2281 aacacaacaa ggttactcat aatatggaaa ttatttaagc tacaacctta taaccataaa
     2341 aatttattaa ccatggtaat agctgctgtt tgctttttta ttgtatatat ttctccatct
     2401 ttaaacaata tatatgccga tgctatttta aaaactgtag tttttatgcc attatatata
     2461 tttttgattt tgaaactaaa aatttcagaa gattttaacc agttgctcct ttcatttata
     2521 gaaaaaataa aaattagaac taaaaaataa tgaagaatac aattatatca tttattcctc
     2581 caattcttat agagttaggt aaaaaaacag gaatactaaa agacaagagc aaagtaatta
     2641 tttacaaaaa aaacgaaaat gacacacaag atttagatat gtattttgac cctgagtatg
     2701 ctaaaatatt agactcttgg ggcgaaaaaa caacttggat agaaatacaa atgttgcttg
     2761 caaattgtaa aggaaaagtt ttagatatag cttgtggcag tggtgttact atgagaattt
     2821 tgaataagta taaagattta gatgtgcatg gttgcgatat ttctgatttg ttaattaaga
     2881 aggctgttag ccttggtatt gcagaaaata atttaaaagt taccgatgcc actaaaatgg
     2941 attatgcaga tttagctttt gactattctt attctattgg ttccttggaa cattttacag
     3001 aaaaaggaat ttctgaattt attgcagaag ctgcaagggt taccaaatat acatctatgc
     3061 acatgatgcc tacttctaaa agtggtacta acgaaggttg gatgagtact tctacaaccg
     3121 ttcaaacctt tcacaacaat tctgacgaat ggtggatgaa caaatacaaa caaagtttta
     3181 gtgaagtaat aatgctcaac tccagatggg acgatggcat ttcttatggt cgttggttta
     3241 tttgtaaaaa ataaaaatat gattttaaga aagataatta aatatttaac taaaactaaa
     3301 attccagcaa atgttattgt tggaaaagat agtgttgtaa taggtcctgt ttctattcat
     3361 actacatctt ccataaccat aggaaacgat tgtttaattg aaggcattgt aaccacacat
     3421 acaccacaag ctaaaataga aattggcaat gaggttttta taggtaataa tagtttttta
     3481 ggctgtgccg atactataat tattgaagat aaagtactgg tttctttcga ttgtgttata
     3541 caagataacg atagccatag cactatttca agtgaaagat ttacagatac caaagattgg
     3601 aaaaatggca gacaacacaa ctgggattta acgcacaaaa aaccaataca catcaaaaaa
     3661 ttagcttgga ttggtgccag agccattatt ttaaaaggtg taaccattgg cgagggagct
     3721 attgttgcag caggaagtgt tgttaccaaa gatgttgctc cttacaccat tgttggtggt
     3781 aatcctgcaa aatttattaa agaaacaacc taacaatgta tggcttatag ttttcca
//
LOCUS       DAOQHP010000215         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14652_length_3836_cov_3.554086, whole genome shotgun sequence.
ACCESSION   DAOQHP010000215 DAOQHP010000000
VERSION     DAOQHP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_3836_cov_3.554086"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PLY81_07330"
     CDS             complement(<1..1825)
                     /locus_tag="PLY81_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNF30076.1"
                     /translation="MKHYSSKPIFKRIPALRLLIPLVAGILVQFHFKIGLNFFLYVAV
                     GSLLLFGIFFFFKPTIKYSVRWLTGISITLLIIALGGCISYIRNIENNNLWVGKFYKN
                     NVPVLLTLQEPIVEKPKSYKALAQINALYINNNWQLTKGNALIYFRKDSSKPSLQYGS
                     QIIVIKNLLPIINAGNPGGFNYAQYCAFQKIHFQVFLNDKEYAVLPNLNTNYFDNLIL
                     KTKLKVLKILRKNITEPNQLSIAEALLIGHRDDLDRDLVQSYSNTGVVHIIAISGLHL
                     GMIYGLLILLFKPFRRFKSANFLKPIFILLVLWGFCFVAGAAPSILRSAVMFSFIVVG
                     EAMSKKTTIYNSLAASAFLLLLINPFNLWDVGFQLSYCAVLSIVAFQKKIRNLFYIKN
                     KLIRFLWNLNAVTLSAQVLTLPIVLYHFHQFPTLFIVTNLIAVPFSGLILYMEILLIL
                     INSFNTLSLILGNVIGYCINLMNKFILFADSLPFSVLPSIQISIVQACILFASIICFW
                     IWDNSKKIKWMVIALSFLAAFFIVRCIDFINKSNQQKIIVYNVPNHTAIDLIEGRNYQ
                     FIGDTILTEDGFLRNFHIKPSRILHRVSPNNQLPHFSIQNNI"
     gene            complement(2023..2505)
                     /locus_tag="PLY81_07335"
     CDS             complement(2023..2505)
                     /locus_tag="PLY81_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30077.1"
                     /translation="MKFHQSILLLIAVSFLACNTATTQTSSIENSDSNKDNQQIKELK
                     ENRHYRGAITGGMKGDSIFFDVTNNGTRLENLTFKGYWRCNGKLEMLRAAGPDGFFTL
                     VNNKVDDHISEPPNGGSTAWRFNLSATINGNTASGTFSMNINNLGCNTGKLQWTAVAL
                     "
     gene            2656..2982
                     /locus_tag="PLY81_07340"
     CDS             2656..2982
                     /locus_tag="PLY81_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNF30078.1"
                     /translation="MNVEQLYEYVNSKEGVEECFPFGEETLVFKVKGKMFLLVALDES
                     PLRFNAKCNPELALELREQFPQNVLPGYHMNKKHWNTIIVDGIIPNNQIKEFINHSYN
                     LVSSKK"
     gene            complement(2997..>3836)
                     /locus_tag="PLY81_07345"
     CDS             complement(2997..>3836)
                     /locus_tag="PLY81_07345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNF30079.1"
                     /translation="IGANGLQHAPLLEVIGLLLIFSAMLFKVSAAPFHFWAPDVYDGS
                     PSVFSSFMMTIVKAAGFIALINLFYIKADSLAQLWKILLFIVIIVTLVVGNITAIFQQ
                     SVKRMMAYSSIAQAGFMLFALYAINDVSKEGILLYAVAYTLATVGIFAILVKIEDYTL
                     EGYNGLAKTQPMLAFVNAIFVFSLAGIPLTAGFFAKYYMLAAVIKTGGNAGIVLVIIG
                     VIFAAVSVYYYFKILQAMYFNEGDAKVNNVSAGFKYGLFLLALTIIFIGIFPDTLINW
                     FYF"
BASE COUNT         1460 a          600 c          518 g         1258 t
ORIGIN      
        1 ttatattatt ttgaatagag aaatgtggaa gttggttatt tggagaaacc ctgtgtaata
       61 ttctagaagg ttttatatga aagttcctta aaaatccgtc ttccgttaaa atagtatcac
      121 caatgaattg ataatttctt ccttctatta aatcaatagc agtatggttg ggtacattat
      181 atacaattat tttttgttga ttagatttat taataaaatc tatacaacga acaataaaga
      241 aagcagcaag aaaagataat gcaataacca tccattttat ttttttacta ttatcccaaa
      301 tccaaaagca aatgatagat gcaaataaaa tacaagcttg aacaatactt atttgaatgg
      361 aagggagaac agaaaaaggt aaactatcgg caaacaaaat aaatttgttc attaaattaa
      421 tacagtagcc aattacattg cctaaaatta agctaagagt gttaaatgaa ttaattagaa
      481 taagcaaaat ttccatatat aatattaaac cagaaaatgg aacagcaatt aaattggtta
      541 ctataaataa agtaggaaac tgatgaaaat gatacaacac aataggtaga gttagaactt
      601 gtgcagacag agttactgca tttaagttcc ataaaaacct gatgagttta tttttaatgt
      661 aaaacagatt tcttattttt ttctgaaaag caacaatact cagtactgca caatatgaaa
      721 gttgaaagcc tacatcccaa aggttgaatg gattaattaa taataacaga aatgcagagg
      781 ctgctaagct attgtaaata gttgttttct tactcatagc ttctccaacc acaataaaag
      841 aaaacattac tgcacttctt aaaatagatg gtgctgcacc agcaacaaaa cagaagcccc
      901 ataatacaag taaaataaat attggcttta aaaagtttgc actcttaaac cttctaaagg
      961 gtttaaataa taaaattagt aacccataaa tcatgcctaa atgcaaccca cttatggcaa
     1021 taatatgtac cactcctgta ttactataac tttgcacaag gtctctatct aaatcatctc
     1081 tatgtccaat tagcaaggct tctgcaatgc ttaattgatt tggttctgta atattttttc
     1141 ttaatatttt taatactttt agtttggttt ttaaaattaa gttatcaaaa taattagtgt
     1201 ttaaattggg taaaacagca tattccttat cgtttaaaaa aacttgaaaa tgaattttct
     1261 gaaaagcaca gtattgagca taattaaagc ctcctggatt cccagcattt attattggta
     1321 acaaattttt tatcacaata atttgtgaac catattgcaa actgggtttt gaactatctt
     1381 ttctaaaata aattaatgca ttgccttttg taagctgcca attattatta atatataaag
     1441 cattaatttg ggctaaggct ttatacgatt ttggtttttc tacaattggc tcttgtaatg
     1501 taagtagtac aggtacatta tttttataaa attttccgac ccataaatta ttgttctcaa
     1561 tatttcttat atagcttata cagccaccta aggcaattat taataaagta atactaatac
     1621 ctgttaacca tcttacacta tatttaattg ttggtttaaa gaagaaaaaa atgccaaata
     1681 ataatagact gccaactgct acataaagaa aaaaatttaa acctatttta aaatgaaact
     1741 gcactaaaat accagcaacc aaaggaatta ataaacgtag agcaggtatt cgtttaaaaa
     1801 ttggtttact ggaatagtgc ttcattgcaa ctatttattt ggatgaataa aatagaaaat
     1861 gaaagttcta ctaaaaatta caacccaatt taatacatta tctgcaaata atattttatt
     1921 tagtattaat agaatattaa agagtgtaac acaaattgca atattgtaaa ataaaacaag
     1981 attacacttt tagagattat acacccaaat acagtttaag ttttataaag ctactgcagt
     2041 ccattgtaac ttacctgtgt tgcatccaag attattgatg ttcatactaa aagttccaga
     2101 agctgtatta ccattaatag ttgctgacaa attaaaacgc catgctgtag aaccaccatt
     2161 tggaggctcg ctgatatgat catcaacttt attatttact aatgtaaaaa aaccatcggg
     2221 gcctgcagct cttaacattt ctaattttcc attgcaacgc caatagcctt taaaagttaa
     2281 gttctccaac cttgtaccat tattagttac atcaaaaaaa atagaatctc ctttcattcc
     2341 acctgtaatg gctcctctat aatgtctatt ttctttcaac tcttttatct gttggttatc
     2401 tttatttgaa tcactatttt ctatgcttga cgtttgagta gtagccgtat tacatgctag
     2461 aaaagatacg gcaattagta gtaaaattga ttggtgaaat ttcatagtaa tatttttgcg
     2521 ataaagataa tgtatagaaa aatatcacca tcaagaaaaa atattcatgt cttcaagtat
     2581 ctttcataat attagtttgc tagttggtta aacttcttca ggtttcacta actatttttt
     2641 atttactttt attttatgaa tgtagagcag ctttatgaat atgttaatag caaagaagga
     2701 gtagaagaat gttttccatt tggcgaagaa actttggttt ttaaagtaaa ggggaaaatg
     2761 tttttattag tggcattaga cgagagtcct ttacgattta atgcaaaatg taatcctgaa
     2821 ttagcattag agctaagaga acaattccct caaaacgtac tacctggcta ccatatgaat
     2881 aaaaaacact ggaacactat tattgttgat ggcataatac ccaacaatca aattaaagag
     2941 tttattaacc actcttataa cttggttagt tcaaaaaaat aaaaacttta aaatcgttaa
     3001 aaataaaacc agttaattaa agtatctggg aagataccaa taaaaatgat ggttaatgct
     3061 aataaaaata aaccatattt aaaccctgca gaaacattgt ttacttttgc atctccctca
     3121 ttaaaataca ttgcctgtaa tattttgaaa taataatata cacttacagc agcaaaaatt
     3181 actccaataa ttaccagaac gattcctgca ttacctcctg ttttaataac tgctgccaac
     3241 atataatatt tagcaaaaaa accagccgtt aatggaatac ctgctaagga aaaaacaaaa
     3301 atagcattta caaatgcaag catgggttgt gttttagcca atccattata tccttctaaa
     3361 gtataatctt caattttaac caatatagca aaaataccaa ctgttgctaa agtgtaggct
     3421 acagcatata ataaaatacc ttctttagat acatcattta ttgcatataa agcaaataac
     3481 ataaaccctg cttgtgcaat acttgagtaa gccatcatac gttttacact ctgctgaaaa
     3541 attgctgtaa tattacccac caccagagtt acaataataa ctataaataa taaaattttc
     3601 cataattgtg ccaggctatc tgctttaata taaaataaat taattaatgc aataaaacct
     3661 gccgctttta caattgtcat cataaacgat gagaaaacac ttggcgagcc atcataaaca
     3721 tcgggtgccc aaaaatgaaa aggagcagct gacactttaa atagcatggc tgaaaaaatt
     3781 aataataacc ctataacctc taataaaggt gcatgctgta aaccattggc accaat
//
LOCUS       DAOQHP010000216         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14711_length_3827_cov_4.627519, whole genome shotgun sequence.
ACCESSION   DAOQHP010000216 DAOQHP010000000
VERSION     DAOQHP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14711_length_3827_cov_4.627519"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLY81_07350"
     rRNA            complement(<1..435)
                     /locus_tag="PLY81_07350"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(558..1331)
                     /locus_tag="PLY81_07355"
     CDS             complement(558..1331)
                     /locus_tag="PLY81_07355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNF30080.1"
                     /translation="MFNTIFFSSPLEQFQVLPIIPIQFGMFDFSITNETVILGLVLFI
                     FISFIFSLLKEDNTFYIIPNKWQAVVEVVYKLILSLVVDNIRDKKGQLFFPLVFSIFL
                     FVLSLNLIGLIPYSFTLTSHLIVTLALSLAVFIGINIVCIRIHGIHFFSLFLPPGTSI
                     VLAFLLVPIELISYVFKPVSLSIRLFANMMAGHTLLKVIAGFAATLMAQSGILFVLHY
                     VPLLILIPLFGLELGVALIQSFVFSILICIYLNDSINLH"
     gene            complement(1383..2186)
                     /locus_tag="PLY81_07360"
     CDS             complement(1383..2186)
                     /locus_tag="PLY81_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF30081.1"
                     /translation="MANFKNNQKHSFHLVDPSPWPLIGGFSAFMLTFGGVLYMHGYSG
                     GSFLWKFGLLMILFVMYSWWRDIIREGTFEGQHTSSVQSGLRLGVILFIVSEVMFFVA
                     FFWAFFHSSFNPTPAIGGVWPPAFIATLDPWKIPLLNTILLLSSGASVTWAHHSIVLG
                     DKAQACIALIVTIILAAIFTGLQGYEYASAPFTIADGIYGSTFYMATGFHGFHVFIGT
                     TFLAVCLGRLYLNHFTREHHFGFEAAAWYWHFVDVVWLFLFITIYWWGS"
     gene            complement(2207..2279)
                     /locus_tag="PLY81_07365"
     tRNA            complement(2207..2279)
                     /locus_tag="PLY81_07365"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2244..2246),aa:Ile,seq:gat)
     gene            complement(2411..2851)
                     /locus_tag="PLY81_07370"
     CDS             complement(2411..2851)
                     /locus_tag="PLY81_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30082.1"
                     /translation="MRKSKNKSLYNIFVGYLTKKGNKIKAKKILDVALLTVARKFNLS
                     IRSLLIKVLKRVGCVVELKTIRMRKNTHIVPFAINQTRKYYLTVRRIMEAVAQDTTNR
                     PITDKLVDELTSILILSKSSRSLKKSKTDLKDAVVHRSNIHFRW"
     gene            complement(2860..3231)
                     /gene="rpsL"
                     /locus_tag="PLY81_07375"
     CDS             complement(2860..3231)
                     /gene="rpsL"
                     /locus_tag="PLY81_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNF30083.1"
                     /translation="MPTLNQLIFAKSRKKKIKRIKTPALRGNPQLKGVCLKVFTRTPK
                     KPNSALRKVAKVRLSTRDRVESYIPGEGHTLQEYSVVLMRGGRVPDLPGVKYHLIRGK
                     YDFKGVKNRKQARSKYGAKKK"
     gene            complement(3253..3324)
                     /locus_tag="PLY81_07380"
     tRNA            complement(3253..3324)
                     /locus_tag="PLY81_07380"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3290..3292),aa:Asn,seq:gtt)
     gene            complement(3329..3712)
                     /gene="rplN"
                     /locus_tag="PLY81_07385"
     CDS             complement(3329..3712)
                     /gene="rplN"
                     /locus_tag="PLY81_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNF30084.1"
                     /translation="MIQKGSYFRVIDNSGAKKACCIHVLGGYRKRYGKTGDIVVVSIK
                     SLRTKRRETSKIKKGDVTKALIVKTKVYRPSYSQECVSFFENSVVLLNNQNKLLGTRI
                     FGPVQKEFRYTKYLRILSLASGVIK"
BASE COUNT         1431 a          638 c          467 g         1291 t
ORIGIN      
        1 acattaaatt ttagagtctt ttcatcctaa aaaagttcta tttcaccttt ccctcacggt
       61 actagttcac tatcggttat tgaatgttct tagatttgag ggaagatcct caactatagc
      121 aaatttgcat aattcgctat aactataaat acttaatatc aaaactacgg gactaacacc
      181 ctcttaggat tattcgttaa tctattaaat atttttatct aatttgtatt cgctcgccac
      241 tactaacaaa gtctcggttg atttcttttt tgagctacta aaatgttttg cttcgcccaa
      301 tttttaaatt tcctaaagaa agaatttctt ctgaagtaat aaggcttcaa caaaaatttc
      361 aagccttctt tatgcacttc acaactttat cattcaatac ctaggcatcc ctttaatgta
      421 ttaaaaatta aaaatagaag ttatgtttcc cgcttacaca gtatctaata acttcttaag
      481 atataacaaa agaatcagaa ttgaagatca tgtacaatct taagattaat ttgtttaaat
      541 ttcttggttt aaattcttta atgtaaatta atagaatcgt ttaaataaat acagattaaa
      601 atactaaaaa caaacgattg aattaaagct actccaagct ctaaaccgaa taatggaatt
      661 aaaattaata acggaacata atgtaataca aataaaatac ctgattgtgc cattaaagta
      721 gcagcaaatc ctgcaattac ttttaataaa gtatgtcctg ccatcatatt agcaaaaagt
      781 cgtattgata aacttactgg tttaaaaaca taagaaatta actcaattgg aactaataaa
      841 aaagctaaaa caattgaggt tcctggaggt aagaataatg aaaaaaaatg aattccatgt
      901 attctaatac aaacaatgtt aataccaata aatacagcta aacttaatgc taaagttaca
      961 attaaatgac tggttaaagt aaaactataa ggaattaatc caattaaatt taacgataat
     1021 acaaataaga atatcgaaaa tactaatgga aaaaataatt gtcctttttt atctctaata
     1081 ttatctacta ctaatgataa tattaattta taaactactt caacaacagc ttgccattta
     1141 ttcggaataa tataaaaagt gttatcttcc tttaataatg aaaaaataaa agaaataaag
     1201 ataaaaagta ctaagcctaa aataacagtt tcattagtaa tactaaaatc aaacatacca
     1261 aattgaattg gaataattgg aagaacttgg aactgttcta aaggacttga aaaaaaaata
     1321 gtgttaaaca taataataat tttttatttt atattttatt aaattaataa ttaaatttat
     1381 tcttaagaac cccaccagta aatagtgatg aataagaata accaaactac gtctacaaaa
     1441 tgccaatacc aagcagcagc ttcaaaaccg aaatgatgct ctctggtaaa atgatttaaa
     1501 tataaacgcc ctaaacatac agctaaaaag gtagttccaa tgaatacatg aaatccatga
     1561 aaaccagtag ccatataaaa ggtagatcca taaataccgt cagcaatagt aaaaggtgct
     1621 gaagcgtact cataaccttg taatccagta aaaatagctg caagaattat agttacaatt
     1681 aatgcaatgc aagcttgtgc tttatctcct aaaacaatag aatgatgagc ccaagttaca
     1741 gatgctcctg aacttaatag cagaatagta tttaataatg gaattttcca aggatctaag
     1801 gttgcaataa aagcaggtgg ccatacacct ccaattgcag gagttggatt gaaacttgag
     1861 tgaaaaaaag cccaaaagaa tgctacgaag aacattactt cagaaacaat aaataaaatc
     1921 acccctaatc ttagaccaga ttgaacagat gaagtatgtt gaccttcaaa agtcccttct
     1981 ctgataatat ctctccacca actatacatt acaaataata tcattaaaag accaaatttc
     2041 cataagaatg atcctcctga gtatccgtgc atatataaaa ctccaccgaa agttaacata
     2101 aatgcagaaa accctccaat tagaggccat ggacttggat ctactaaatg aaagctatgc
     2161 ttttgattat ttttaaagtt tgccataatt tatattttat attttatagg ctcaagtaga
     2221 tttgaactac cgactttacg cttatcaggc gtatactcta acccctgagt tatgagcctt
     2281 ttataatgaa taaatgtaaa aacttaggta cttcttttaa agaataacct ttaacataaa
     2341 gaagttgacc ttgttcattt actagaattc ttctagattt tagaatatta acagttgttt
     2401 ttttcataaa ttaccatcta aagtgtatat tagatctgtg aactacagca tcttttaaat
     2461 cggttttact tttctttaag cttctactag atttacttaa aattaaaata ctagttaact
     2521 catcaactaa cttatctgta ataggtctat tagtagtatc ttgagcaaca gcttccataa
     2581 ttcttcttac agtaagataa tatttacgag tttgatttat tgcaaaggga actatatgag
     2641 tattttttct cattctaata gtttttaatt caaccacaca accaactctt tttaaaacct
     2701 taattaaaag agaacgtata gataaattaa attttctagc aacagttaac aaagctacat
     2761 ctaaaatttt ttttgcttta attttattac cctttttagt aaggtatccc acaaaaatgt
     2821 tatacaacga tttgtttttg ctttttctca taaaatttat tatttcttct tagcaccata
     2881 ttttgatcgc gcctgtttac gatttttaac acctttaaaa tcatattttc cacgtattag
     2941 atgatactta accccaggta aatcaggaac cctaccccct ctcataagaa caacagaata
     3001 ctcttgtaaa gtatgtcctt ccccaggaat atatgattct actctatcac gagtagataa
     3061 tctaacttta gctacttttc taagtgcaga attaggcttt ttaggtgttc tggtaaaaac
     3121 ttttaaacaa acacctttaa gctgaggatt tccacgcaat gcaggagttt ttattctttt
     3181 tatttttttc tttctagatt tagcaaatat taattgattt aaagttggca taataattga
     3241 tataattatc aaatccttgg gtgggaattg aacccacgac caagtggtta acagccactt
     3301 gctctacctc tgagctacca aagagttttt atttaataac acctgaagca agagataata
     3361 tccttaaata tttagtatat ctaaattctt tctgtacagg gccaaaaata cgagtaccta
     3421 ataatttatt ctgattattt agtaaaacta ctgaattttc aaaaaaagac acacattctt
     3481 gcgaatatga aggtctatag actttagttt taacgattaa tgctttagta acatctcctt
     3541 tcttaatttt tgaagtttca cgacgctttg tccttaaact ttttatagag acaacaacaa
     3601 tatcaccagt tttaccgtaa cgttttctat aaccccctaa tacatggata caacatgctt
     3661 tttttgcacc agaattatca ataactctga aataacttcc tttttgtatc ataattttta
     3721 attttatttt taatttacca aatagctttt ttatagcctg gaacaacacc agcacgtaac
     3781 atatctctta ataaaattct agaaatacct aattttctat ctgaaac
//
LOCUS       DAOQHP010000217         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14748_length_3820_cov_3.152988, whole genome shotgun sequence.
ACCESSION   DAOQHP010000217 DAOQHP010000000
VERSION     DAOQHP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14748_length_3820_cov_3.152988"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLY81_07390"
     CDS             <1..624
                     /locus_tag="PLY81_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF30085.1"
                     /translation="LGIHVGAVVTYQDGFDELANGYYVGRAFDNRIGGFMIAEVARLL
                     KENKKKLPFGLYVVNAVQEEIGLRGAEMIARRIKPNVAIITDVTHDTQTPMINKIIEG
                     DVACGKGPSPTYGPAVHNKLLQLVEDTANKKKIPLQMRTVSRSTGTDTDSFAYANDGC
                     PSVLISIPLRYMHTTVEMLHKKDIENTIQLIYETVVALTPKTNLSYL"
     gene            738..1376
                     /locus_tag="PLY81_07395"
     CDS             738..1376
                     /locus_tag="PLY81_07395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNF30086.1"
                     /translation="MIITNSLAEKYAEWLTNNDDELLTQIHQSTSQNHAHAHMLSGHV
                     QGMLLTFMSTLLNPHNILEIGTFTGYSALCLAKGLTVDGQLHTIEIREEDARIAQENF
                     NKSQYSKQLFLHIGNALEIIPTLNKTWDLIFIDADKTGYIKYYEMVLPLLNKNGLIIV
                     DNILFHGQVLEEDIKGKNAIAIDNFNKHIAQDTSVNKVLLTVRDGLLFIKKK"
     gene            1378..2436
                     /locus_tag="PLY81_07400"
     CDS             1378..2436
                     /locus_tag="PLY81_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNF30087.1"
                     /translation="MRWTILIALLINFCSYGIAQTEKANNYISLYKEVAINEMRRSGV
                     PAAIILAQGMLESGYGESELSKKSNNHFGIKCKNDWQGEKTYFDDDEKGECFRVYKSV
                     AESYKDHSDFLKSRSWYAFLFKLPPTDYEGWAHGLKKAGYATANDYAPKLIKIITDYN
                     LQQFTLEGLKNSEANTTTKTDKQDSTTIPYHSEDNTETTEEDSTETSTNETRTNISKP
                     LNQDTLKPKHPIYATDSVFTINHSKVIFSAEGTSLLAIANKYNISLSSLFAFNDMKPI
                     ELIDTFRLIFIEKKLKKGAEDNFISNNNETLYDICQLKGVQLESILQYNKGIKKDVKL
                     SKGFIVYLRPQNKIKKGK"
     gene            2473..3015
                     /gene="hpt"
                     /locus_tag="PLY81_07405"
     CDS             2473..3015
                     /gene="hpt"
                     /locus_tag="PLY81_07405"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNF30088.1"
                     /translation="MQNSISLHNKVFVPFISEQEIADKIQQLATQLKQDYDGKKPLFL
                     AILNGAFMFASDLFKTLEIEAEICFIKLASYKGTKSTGHVITAIGLDTDLTNRHVIIL
                     EDIIDTGKTLHSFLPQVYNQQPASLKIAVLLDKPEPRQFDVPVDYACFVIPNKFVLGY
                     GLDYDGFGRNTKDIFELFEG"
     gene            3085..>3820
                     /locus_tag="PLY81_07410"
     CDS             3085..>3820
                     /locus_tag="PLY81_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNF30089.1"
                     /translation="MNLIRLTILILPLVFLLSCKKHTDTYTNTLYFPPNSSTQWDTMS
                     STEAGFNASKLQALKDYLAITNTKSFMIVKNGKIVVEEYYNSHTSSSTWQWNSAGKTL
                     VATITGIAQQDGFLNINNKVSDYLGNGWTSENLLKENLIKIKHLLTMTSGLNDANQLV
                     IKSNLTYLADAGTRWSYHNVFQKLIDVVASATSQNFENYFNNKLRDKIGMDGFWNFGT
                     IFKIYHSNTRSMARFGLLTLNKGKWNN"
BASE COUNT         1447 a          524 c          613 g         1236 t
ORIGIN      
        1 ttaggcattc atgtgggagc tgttgttacg tatcaagatg ggtttgatga gttggcaaat
       61 gggtattatg taggcagagc atttgataac agaataggtg gatttatgat tgcagaagta
      121 gcaagattat taaaagaaaa caagaagaaa ctaccttttg gtttatatgt agtgaatgca
      181 gtacaagaag aaattggatt gcgtggtgcc gagatgattg caagaagaat aaaacccaat
      241 gtggcaatta ttactgatgt tacacatgat acgcaaaccc caatgataaa taaaattatt
      301 gaaggagatg tagcttgtgg caaaggccca tctcctacct atggccctgc agttcacaat
      361 aaacttttac aattagtaga agacacagcc aataagaaaa aaattccatt acaaatgaga
      421 acagtaagta gaagcactgg tacagataca gatagttttg cttatgccaa tgatggttgc
      481 ccaagtgttt taatttctat tcctttacgt tatatgcaca ctactgtaga aatgctacat
      541 aaaaaagata ttgaaaatac tattcaacta atttatgaaa cagtagtagc attaacgcct
      601 aaaactaatt taagttattt atagttttat acttgatttt tttttctaac tgcttctata
      661 gtataggagc agttttttat ttacaattta agagcaatta aaataatttt aaaaacaaat
      721 gctattagtt ttgtgctatg attattacaa attctttagc agaaaaatat gctgaatggc
      781 ttaccaataa tgatgacgaa ttgctaacac aaattcatca atcaacatct caaaatcatg
      841 ctcatgcaca catgcttagt ggccatgttc aaggtatgtt gttaacattt atgagtaccc
      901 tattaaaccc tcataatatt ttagaaattg gcacttttac aggttatagt gctttgtgcc
      961 ttgcaaaagg attaacagta gatggccaac tgcatactat agaaattaga gaagaagatg
     1021 ctagaattgc tcaggaaaac tttaataaat cgcaatatag caaacagtta tttttgcata
     1081 taggcaatgc tttagaaatt attccaacat taaacaaaac atgggattta atttttattg
     1141 atgctgataa aacggggtat attaaatact atgaaatggt tttgccctta ttaaacaaaa
     1201 atggattgat aattgtagat aatatattat ttcatgggca agtgctagaa gaagatatta
     1261 aaggtaaaaa tgcaattgct atagataatt ttaacaagca tattgcccaa gataccagtg
     1321 taaacaaagt tttattaaca gtaagagatg gtttgttatt tattaaaaag aaatagaatg
     1381 agatggacaa ttttaatagc actattaatt aatttttgta gttatggcat tgctcaaaca
     1441 gaaaaagcaa ataactacat tagtttatat aaagaagttg ctattaacga aatgagaaga
     1501 agtggtgtgc cagcagcaat tattttagca caaggaatgc tagaaagcgg atatggcgaa
     1561 agtgaactta gtaaaaaaag caataaccat tttggaataa aatgtaaaaa tgactggcaa
     1621 ggagaaaaaa cttattttga tgatgatgaa aaaggcgaat gttttagagt gtacaaatct
     1681 gttgcagaaa gttataaaga tcattctgat tttttaaaga gccgttcttg gtatgctttt
     1741 ttattcaaat taccgccaac cgattatgaa ggttgggcac atggattaaa aaaagcagga
     1801 tatgcaactg ctaatgatta tgcacccaaa ctaataaaaa ttattaccga ttataattta
     1861 caacaattca ctttagaggg attaaaaaat tctgaagcaa atactaccac taaaacagat
     1921 aaacaagata gtacaactat tccatatcat tcagaagata atacagaaac aactgaagaa
     1981 gactctactg aaacttctac taatgaaaca agaacaaata tttcaaagcc tttaaatcaa
     2041 gacactttaa aacctaagca cccaatttat gcaaccgata gtgtttttac catcaatcac
     2101 tctaaagtaa ttttttctgc cgaaggcact tctttgctag caattgccaa taaatacaat
     2161 atttctctaa gttctttatt tgcatttaac gatatgaaac caatagagtt aattgatact
     2221 tttagattaa tttttattga gaaaaaatta aaaaaaggtg cagaagataa ttttatttcc
     2281 aataacaacg aaaccctcta tgatatttgt cagttaaaag gtgtacaact tgaaagtatt
     2341 ctgcaataca ataaaggaat taaaaaagat gtaaaattaa gtaaagggtt tattgtatat
     2401 cttagaccac aaaataaaat taagaaaggg aaataaccca ttattctatt atccttaaaa
     2461 tcataaatag ttatgcaaaa tagtatttca cttcataata aagtatttgt tccttttatt
     2521 tcagagcaag aaattgctga taaaattcaa caattagcta ctcagcttaa acaagattat
     2581 gacggcaaaa aacccttgtt tcttgctatt ttaaatggtg catttatgtt tgcatcagac
     2641 ctttttaaaa ccttagaaat tgaagcagaa atttgtttta ttaaactagc ttcctataaa
     2701 ggcacaaaaa gcacaggcca tgtaattact gctattggct tagatacaga tttaactaat
     2761 agacatgtta ttattttaga agatattatt gatactggta aaacccttca ctccttttta
     2821 ccacaagtgt ataaccagca acctgcttcc ttaaaaattg ctgttttatt agataaacca
     2881 gaaccacgtc agtttgatgt acctgtggat tatgcatgtt ttgttattcc taataaattt
     2941 gttttagggt atggtttaga ttacgatgga tttggaagaa atacaaaaga tatatttgaa
     3001 ctttttgaag gctagtttat ttttttaaat ttcacacttt cttttaacct ttttgtaatt
     3061 tgtagtctaa ttgaaaagtt aattatgaat ttgattaggt taaccatact tatacttcca
     3121 cttgtatttt tattaagttg caaaaaacat actgatacct atacaaatac tttatacttt
     3181 ccgcctaata gtagcacaca atgggataca atgtctagta cagaagctgg ttttaatgca
     3241 agtaaactac aagcattaaa agattattta gctattacca atacaaaatc atttatgatt
     3301 gttaagaatg gtaaaattgt tgtagaagaa tattataata gtcatacaag ctcatccaca
     3361 tggcaatgga atagtgcagg taaaacttta gttgcaacaa taacaggtat tgcacagcaa
     3421 gatggatttt tgaatattaa taataaagta tccgattatt taggtaatgg atggacttct
     3481 gaaaatttac taaaagaaaa tcttataaaa attaaacact tgcttacgat gacaagtggc
     3541 ttaaacgatg caaaccagtt agtaataaaa tcaaatttaa cttacttggc agatgctgga
     3601 acaaggtggt catatcataa tgtatttcaa aaattaattg atgttgtagc ctctgcaaca
     3661 tctcaaaatt ttgaaaatta ttttaataat aaattaagag ataaaattgg tatggatggc
     3721 ttttggaatt ttggcacaat ttttaaaatt taccatagca atacacgaag catggcaaga
     3781 tttggtttac ttacacttaa taaaggcaaa tggaataatg
//
LOCUS       DAOQHP010000218         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14768_length_3817_cov_3.418926, whole genome shotgun sequence.
ACCESSION   DAOQHP010000218 DAOQHP010000000
VERSION     DAOQHP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14768_length_3817_cov_3.418926"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..385
                     /gene="rpmI"
                     /locus_tag="PLY81_07415"
     CDS             188..385
                     /gene="rpmI"
                     /locus_tag="PLY81_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNF30090.1"
                     /translation="MPKVKTNSSAKKRFKVTGTGEITFQKAFKRHILTKKSKKRKRNM
                     RKAGVVGSANKDFVQRLLGLK"
     gene            476..820
                     /gene="rplT"
                     /locus_tag="PLY81_07420"
     CDS             476..820
                     /gene="rplT"
                     /locus_tag="PLY81_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNF30091.1"
                     /translation="MPRSVNAVASRARRKRILKQAKGFYGKRKNVYTVAKNIVEKGMT
                     YSYVGRKLKKREYRQLWIARINAAVREEGLTYSQFINKLSVKGIDLNRKVLADLAMNN
                     PESFKALVASVK"
     gene            948..2378
                     /locus_tag="PLY81_07425"
     CDS             948..2378
                     /locus_tag="PLY81_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNF30092.1"
                     /translation="MTNSYTSLVHQTFHFPQEDFNISDKNDFLEFNGINLKPIIEKYG
                     TPLKLTYLPKIGMQINKAKQMFANAFKKHKYEGKYNYCYCTKSSHFSFVVEEALRHNI
                     NIETSFAYDIEIINQLYKRRKITKDVFIICNGFKQKAYTSRIAKLINSGFKNVVPILD
                     NKEELKAYKKTVKQPFGIGIRVAAEEEPNFPFYTSRLGIRAKDILEFYVDEIEGNESK
                     FYLKMLHVFLNKGIKDDIYYWSELNKVINLYCQLKKICPELDSINIGGGFPIKHSLGF
                     EYDYQFMINEIVSNIKKSCKKAKVPVPNIFTEFGSYTVGESMAHIYSIIGEKQQNDRE
                     TWYMIDSSFITTLPDTWGIGEKFLMLPINKWDNEYQRVVLGGITCDSHDYYDSEEHIK
                     EVFLPKIKSNKPEDDANKEPLYLGFFHTGAYQDQISGYGGIKHCLIPSPKHVIIEHDK
                     NGNLIDWVYAKEQSASSMLKILGYLK"
     gene            2398..3132
                     /locus_tag="PLY81_07430"
     CDS             2398..3132
                     /locus_tag="PLY81_07430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF30093.1"
                     /translation="MKRVIILIAMCPLIAIAQQTKTTPKKVVQQTKTTSSKPTKGFIL
                     KTGLDSLSYALGTRIALNLNANHIDTLSYNAFIEGLKDIFKKSNLKFDENTINQVINT
                     KVQELSVQKASKEKENGKKFLEANKKKQGVITTSSGLQYEVITVGNGAKPTLQDTVVC
                     HYVGTLVNGFEFDNSRKRGEPIQFPVTGVIKGWTEALQLMPKGSKWKLYIPSDLGYGD
                     RGAGNDIPGGSALIFEVELIDIKAKQ"
     gene            3282..>3817
                     /locus_tag="PLY81_07435"
     CDS             3282..>3817
                     /locus_tag="PLY81_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30094.1"
                     /translation="MKKQFLLFFILFSVSFIKINAQTDVIIGAGSVTGTTANGAATDA
                     GPIYCTGSSSSFIYSKHFIVYTAAELSNAGIQPGMLITNLAWNKANDAAYSASTAVVF
                     DIYMKNSSATGVPTPVPQDFASLVSGATLVYASTTQSFPSTIGWVNFTLTTPFLYTGG
                     AIEMATNWDVSAGGSTASG"
BASE COUNT         1454 a          533 c          606 g         1224 t
ORIGIN      
        1 aatgaatggg tgaaaaaaca tagaaatttc taatacttct aatataattg ctatattctt
       61 acttttttgt tgtttatttt agtataaact cctaattttg caatcccaaa aatattaccc
      121 acaaggctca acaagtttct cgtctgttcg agaacgccag cagggtgtaa tcttaaccag
      181 attatttatg cctaaagtaa aaacaaactc aagtgcaaaa aaaaggttta aagtaaccgg
      241 cacaggcgaa attacttttc aaaaagcgtt taaacgccac atcctaacta aaaaatctaa
      301 aaaacgcaaa cgcaacatgc gtaaagcagg tgttgtaggt tctgctaata aagattttgt
      361 tcaaagatta ttaggattaa aataaacact taaacaagaa aagtaattaa ccaaattcat
      421 ttcacttttt cggtttttaa ttattaaatt ttaccgaaat taaattaaac caattatgcc
      481 acgttcagta aatgcagtag cttctagagc tcgcagaaaa agaatattaa aacaagcaaa
      541 aggcttttat ggcaaacgta aaaatgtata tacagttgcc aaaaatattg tagaaaaagg
      601 gatgacttac agttatgtag gtcgtaaatt aaagaaaaga gaatatcgtc aattatggat
      661 tgctcgtatt aacgcagcag ttagagaaga aggtttaact tatagccaat ttatcaataa
      721 gttatcagtt aaaggaatag atttaaacag aaaagtactt gcagatttag ctatgaataa
      781 tcccgaaagc tttaaagctt tagtagcttc tgtaaaataa tttttttaaa taaagttata
      841 tttttagccg aagaaaattt tcttcggctt tttaattttg catacttcaa aattgtattt
      901 ttgttactta ctttttaatt ttaatgtttt aatagaagtt attaagaatg acaaacagct
      961 acacctccct ggtgcatcaa acatttcact ttcctcagga agactttaac attagtgata
     1021 agaatgattt tttagagttt aatggcatta accttaaacc aataatagaa aagtatggaa
     1081 cgccattaaa actaacctat ttacctaaaa taggaatgca aataaataag gcaaaacaaa
     1141 tgtttgccaa tgcttttaaa aaacataaat acgaaggaaa atacaattat tgttattgta
     1201 ccaaaagctc tcatttttct tttgtggtgg aagaagcatt aaggcacaat attaacattg
     1261 aaacatcttt tgcctacgat atagaaataa taaatcagtt atataaaaga agaaaaatta
     1321 caaaagatgt atttattatt tgcaatggtt ttaaacaaaa ggcctacact tctagaatag
     1381 ctaaactaat aaattctgga tttaaaaatg tagtaccaat attagataat aaagaagagt
     1441 taaaagcata taaaaaaact gttaaacaac cttttggtat tggaattaga gtggctgcag
     1501 aagaagaacc caatttccct ttttatacat ctagattagg aataagagca aaagatattt
     1561 tagaatttta tgtagatgaa atagaaggca acgaatcaaa attttattta aaaatgctac
     1621 atgtgttttt aaataaagga ataaaagatg atatttatta ttggagtgaa ttaaataagg
     1681 taattaacct ttattgccag ttaaagaaaa tttgtcccga attagattca ataaatatag
     1741 gtggtggctt ccctataaaa cattccttag gctttgagta cgattatcaa tttatgatta
     1801 atgaaattgt aagtaatatc aaaaaatctt gcaaaaaagc taaagtaccg gtacctaata
     1861 tttttacaga gtttggtagt tatacagttg gcgaaagtat ggctcatatt tatagcataa
     1921 taggcgaaaa acaacaaaat gatagagaaa cctggtacat gatagattct tcttttatta
     1981 ccactttgcc agatacttgg ggtataggag aaaaattttt gatgcttcct attaataaat
     2041 gggataatga ataccagcgt gtagttttag gtggtattac atgtgatagc catgattatt
     2101 acgatagtga agaacatata aaagaagttt ttttacccaa aataaaaagc aataagccag
     2161 aggatgatgc caacaaagaa cctctttatt taggtttctt tcatacaggt gcctatcaag
     2221 accaaattag cggttatggt ggtataaagc attgtttaat tccttctcca aaacatgtaa
     2281 taattgaaca tgataaaaat ggtaacttaa tagattgggt ttatgcaaaa gaacagtctg
     2341 cctccagtat gcttaaaatt cttggttatc taaaataaat ttaaaaaaca aaattaaatg
     2401 aagagagtaa ttatcttaat tgctatgtgt ccacttatag caattgcaca acaaacaaaa
     2461 acaacaccta agaaagttgt acaacaaaca aaaacaacat catcaaagcc aacaaaagga
     2521 tttattttaa aaacaggatt agattcttta agttatgcat taggcactag aatagctttg
     2581 aacttaaacg caaaccatat tgatacctta agctataatg cattcattga agggttaaaa
     2641 gatatattta aaaaatcaaa cttaaagttt gatgaaaata ctatcaatca agttatcaat
     2701 accaaagtgc aagaattatc cgtacaaaaa gcatctaaag aaaaagaaaa tggaaaaaaa
     2761 tttttagaag ccaataaaaa gaaacaagga gtaattacaa caagtagtgg tttacaatat
     2821 gaggtaatta ctgtaggtaa tggagcaaaa cctactttgc aagatactgt tgtttgtcac
     2881 tatgtaggca ctttagtaaa tggatttgaa tttgataata gtagaaaaag aggcgagcct
     2941 attcaatttc ctgtaactgg agtaattaaa ggttggacag aagcattaca acttatgccc
     3001 aaaggaagca aatggaaatt gtatattcca tctgatttag gctatggcga cagaggagca
     3061 ggaaatgata tacctggtgg ttctgcttta atttttgaag tagaattgat agatattaag
     3121 gcaaaacaat agctacagta aacttgcata cttctattga taaactattg aattgtatat
     3181 acattttata ttggtataaa ttatatactt acacttgctt taatttttgt atttttaatg
     3241 tcctgttatt tattaaattt tcttaaccaa actaactgat tatgaaaaaa caatttttac
     3301 ttttttttat tctattctct gtaagtttta ttaaaataaa tgcacaaact gatgtgatta
     3361 ttggtgcagg ttcagtaact ggaactacag ctaatggtgc tgctacagat gcagggccta
     3421 tatattgtac aggaagttct agttctttta tatactccaa acactttatt gtttacacag
     3481 ctgcagaatt aagtaatgca ggcattcaac ctggtatgct aattactaac ctagcatgga
     3541 acaaagcaaa tgatgctgct tactctgcat ctactgctgt tgtatttgat atttatatga
     3601 aaaactcttc agcaaccgga gttccaactc cagtgccaca agattttgca tcattagttt
     3661 ctggtgctac tttagtttat gcatctacta ctcaaagttt tccatcaaca attggttggg
     3721 ttaattttac attaacaact ccttttttat atacaggtgg tgcaattgaa atggcaacta
     3781 attgggatgt ttctgcagga ggttcaactg cttcagg
//
LOCUS       DAOQHP010000219         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14839_length_3804_cov_5.039744, whole genome shotgun sequence.
ACCESSION   DAOQHP010000219 DAOQHP010000000
VERSION     DAOQHP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14839_length_3804_cov_5.039744"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..269)
                     /locus_tag="PLY81_07440"
     CDS             complement(63..269)
                     /locus_tag="PLY81_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNF30095.1"
                     /translation="MNNWYLLYSTRNMVEASILQGMLEENQIPVQLLNKLDSSYMAFG
                     YIELYVPVHLKETAQLLLQNGLLN"
     gene            complement(269..1216)
                     /locus_tag="PLY81_07445"
     CDS             complement(269..1216)
                     /locus_tag="PLY81_07445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNF30096.1"
                     /translation="MQLQPKIRYSSQVAILAGLIGVSFIIASFAAAGFWRVFTGKSIF
                     TMQEDMANPAFANAARWTQLIAAAIMFFIPAIIYARIVNPNPFQQLGFNSVISGKQFF
                     LVLLIALCGMALSGSLGTLNEMIPISKEWAAKFKNAEDAYNKQVLIIAKMNSFKEYLF
                     AMVVIALAPAIFEEVLFRGALQRLFQHWFKNISLAIVVTSIIFSIVHFSYYGFLARAG
                     LGIILGLIFYYSRNIWLCIWAHFINNGLAVTALYVLGLQGKNPETALKDNFPIIYGAA
                     ALIIIIFLLLTFKKESNSIGANEIYDGSLEKLKSNNPFE"
     gene            1597..3018
                     /locus_tag="PLY81_07450"
                     /pseudo
     CDS             1597..3018
                     /locus_tag="PLY81_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            3141..3719
                     /locus_tag="PLY81_07455"
     CDS             3141..3719
                     /locus_tag="PLY81_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF30097.1"
                     /translation="MIKFKEAKEFITQKQLEEIPKHFTYHSLVHILIVYESCKVLAEG
                     EGITGIDLQLLLTAALFHDSGFIIGPKNHEETSCEFARQYLPQFNYTNEQIEHICDMI
                     MATKIPQSPKDHLGQILADADLDYLGRDDFYTNGNKLFEELKTLNVLKTEREWYQLQI
                     NFLEKHNYFTATAIRLRKEKKHIHLNELKNKL"
BASE COUNT         1513 a          531 c          617 g         1143 t
ORIGIN      
        1 taaaaaagct gaacccctaa ttattttact tacacacttt atgggatagt gtcaatttat
       61 acttaattaa gcagcccatt ttgtaaaagc aattgggctg tttcttttaa atgaactggt
      121 acatataatt ctatataccc aaaagccata taactgctat ctaatttgtt taataattga
      181 acgggtattt gattttcttc taacatacct tgtaaaatac ttgcctccac catgtttctt
      241 gtactatata ataaatacca attattcatt actcaaacgg attattgctt tttaattttt
      301 ctaatgaacc atcataaatt tcatttgcac caatactatt actttctttt ttaaacgtta
      361 gtaataaaaa gataataata atcaatgctg cagcaccata aattatagga aaattatctt
      421 tcaaagccgt ttctgggttt ttaccctgca agccaagaac atacaaggca gtaacggcaa
      481 ggccattatt aataaaatga gcccaaatac ataaccaaat atttctacta taataaaaaa
      541 ttaatcctaa aataataccc aaacctgccc tggctaaaaa gccataataa ctaaaatgaa
      601 caatgctaaa aataatggaa gtaaccacaa tggcaaggct tatattttta aaccaatgtt
      661 gaaataatct ttgtaatgca cccctaaaca aaacctcttc aaatattgca ggagccagtg
      721 caattaccac cattgcaaac aaatattctt taaaactatt cattttggca attattaaaa
      781 cctgcttatt atatgcatcc tctgcatttt taaactttgc agcccactcc ttagaaatag
      841 gaatcatttc atttagtgta cctaagctac cacttaatgc cataccacat aatgcaatta
      901 acagaactaa aaaaaattgc ttaccactaa taacactatt aaaacctaat tgttgaaaag
      961 gattggggtt tacaatacga gcataaataa tagcaggaat aaaaaacata attgcagcag
     1021 caataagttg tgtccatctt gcagcattag caaatgctgg gttggccata tcttcttgca
     1081 tggtaaaaat acttttacct gtaaaaactc tccaaaaacc agctgcagca aaacttgcta
     1141 taatgaacga tacgccaatt aatccagcca atatggcaac ctggcttgaa tatctaattt
     1201 taggttgtaa ttgcatttta atatattgaa gataatcaga taatatattt tgtattcatt
     1261 caaaaatatg ttgaagatta aagaataaaa attaaattat attaatgcct ttaaaatttt
     1321 caaataatta attaaacaat atagtagtgt tggttgtttt taaaaactaa tgaccaataa
     1381 atagcaatca gaataatagt agcaagaaaa acattaaaga gcatgtggtc tttattggta
     1441 aattttcttt atttttgata tgctgcaaat taactattta aagtctgatg ttcttgtttg
     1501 tataaaaaat tatccataca aattaatcat atgaaccaag ggctttcaaa aaaacgggta
     1561 aagaaaacaa atcctaaagc gttaggtatt agtattatgc aagaagaaaa caaaaggttt
     1621 caggtaatat gtagttcgaa aaatgatgct atcattacct ctgatgaaac caagctaatt
     1681 acattttgga ataagggggc agaaagtatt tttggttata ctgcagagga ggctgtagga
     1741 aagcccatga ctttaataat tccaatgcaa tatcaacaaa ggcataatga tggaatagaa
     1801 agaatgaacc agcgaaaaaa accacgtgtt ataggaaagg ttttagaatt aagtgccgtt
     1861 agaaaaaatg gcgaagagtt tccgatagaa cttactttag gcagttggga tagtgatggt
     1921 aaaaggtatt atagcggaat tataagagac atttctgcaa aaaagaaagc agaaaaaatt
     1981 atgcttgaag acaaaagaaa aatagaggag caaaataaaa ggtttcaagt aatttgtagt
     2041 tcaaaaaacg atgctatcat tacctctgat gaaagcaagc taattacatt ttggaataaa
     2101 ggggcagaaa atatttttgg ttatactgca gaggaggctg taggaaagcc catgacttta
     2161 ataattccaa tgcaatatca acaaaggcat aatgatggaa tagaaagaat gaaccagcga
     2221 aaaaaaccac gtgttatagg aaaggtttta gaattaagtg ccgttagaaa aaatggcgaa
     2281 gagtttccga tagaacttac tttaggcagt tgggatagtg atggtaaaag gtattatagc
     2341 ggaattataa gagacatttc tgcaaaaaag aaagcagaga aattaattat agaagaaaag
     2401 aaaaaatcgg atgagttatt attaaacata ttacccaagc tggtggcaga tgaattaaaa
     2461 aatactggaa ttgccactgc taaaaaccat aaaaatgtaa caattctttt ttcagatttt
     2521 gagggtttta ccacattagc atcttctatt tctgcagcag aattggtaaa agaattaaat
     2581 gaaatttttg gacagtttga tgatatttta gaatccttat agatagaaaa aattaaaaca
     2641 attggcgatg cttattttgc agcatgtggc ttgcccgaaa aaaatacaga acacgccgaa
     2701 cgctgtattg aagcagcaaa acaaatgttt agatatttag aagtaagaaa taaaaaaact
     2761 gaaattaaat ggaaaatgag agtaggcatt cattctggcc cagttatggc aggcgtggta
     2821 agtaaaaaga aatatgctta cgatcttttt ggcgatacag taaatattgc cagcagaatg
     2881 gaaagcaatg gtgaagttgg aaaaattaat atttccgaat ctacctatca gttaataaag
     2941 aaaaaatata agtttacacc aagagggcat gtaaaaacaa aaggaaaggg ggagttaaaa
     3001 atgtattttg ttgattaaaa tgttttcaaa aatatagtat ttataaggat agggtttatt
     3061 acaaaaaatt tgtttgtatt taaaaataaa ttattgataa gttggaaagt tttcaataat
     3121 tttaaaaata aatttaatta gtgataaagt ttaaagaggc aaaagagttt attacacaaa
     3181 agcaacttga agaaatacca aaacacttta cttaccatag tttagtgcat attttaattg
     3241 tttatgaaag ctgtaaagtg ttggccgaag gagaaggaat tacagggatt gatttacaac
     3301 tattgctaac cgctgcactt tttcatgatt cgggttttat tattggccca aaaaatcatg
     3361 aagaaacatc ttgtgagttt gcacgtcaat atttaccaca atttaattac accaatgaac
     3421 agatagagca tatttgcgac atgattatgg caacaaaaat accacaatca cctaaagacc
     3481 acttagggca aatacttgca gatgccgatt tagattattt aggcagagat gatttttata
     3541 ccaatggtaa taagttattt gaagaattaa aaacactgaa tgttttaaaa accgaaagag
     3601 agtggtacca actacaaata aattttttag aaaaacataa ttattttact gctactgcaa
     3661 ttaggcttag aaaagaaaaa aagcatattc atttaaacga attaaaaaat aaactataat
     3721 caaaacatca tttatagtaa attagaaaat gaggaagcat agtattacca atcattttaa
     3781 agacagtata tatattgctt tagg
//
LOCUS       DAOQHP010000220         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14896_length_3795_cov_3.316578, whole genome shotgun sequence.
ACCESSION   DAOQHP010000220 DAOQHP010000000
VERSION     DAOQHP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14896_length_3795_cov_3.316578"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PLY81_07460"
     CDS             <1..1593
                     /locus_tag="PLY81_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF30098.1"
                     /translation="PSIFGFAIDEEKDLLAFETYSEMTLMKLSTQKILWRDNPLESSI
                     KISYQPNGIAFGTKSEFYTIANNLNNCYFTTYTGNKPVEILNNHLYKADKSAINNLAF
                     VLKEKGFLPEDFDMYFNRPDSVTFEAGSTNATYNELLSKVVEKRKRKFNTQNITEVLS
                     NSPTCTIINKNEIPDVTKENHILLQIKASSPTSAAITAIHILNNGVPVFGKAGFTNNN
                     QPITEEQKIDVVLTNGINNVQVYVSDNTALVSASESIIISSDYIEVTEPKQHFIGIGI
                     NQFSNPDYNLNWSVKDIRDLALKLKSKFPNIIIDTLFDENVTKEKILAVKKKLLQLNE
                     NDKVIVSYSGHGVLNKDFDYFLSTYSINFEKPEEKGLSYNDLENLLDSIKPRQKLMFI
                     DACHSGEVDKEEIEKVEASKKELEKNGVVTKSTIKVKPKKNIGMANSFELMQNLFVNV
                     GKGTGATIISAAGGMQYAQERGDLKNGVFTYSVLEAFDKNTTLKVSELKKIVGERVIQ
                     LTNGLQKPTSRNEANNYDWVVW"
     gene            1664..1870
                     /locus_tag="PLY81_07465"
     CDS             1664..1870
                     /locus_tag="PLY81_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNF30099.1"
                     /translation="MRYACLIVLFFILFFLRAQEPTLVTLIGHSETITLTKFSPNERL
                     MLTASEDALYKLNFATTGNKKVLV"
     gene            1887..3152
                     /locus_tag="PLY81_07470"
     CDS             1887..3152
                     /locus_tag="PLY81_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNF30100.1"
                     /translation="MNKIFATIITIGDELLIGQVIDTNSAFIAQELNKIGISVKQRVS
                     IADEEEAIFTALDNAFIHSNIILITGGLGPTSDDITKPTLCKYFGGKMTMHQPTLQHV
                     TYLFEQVFKRPMIERNVQQAMVPDVCTVLKNEVGTAPAMLFKKNNTICIAMPGVPFEM
                     KWIMQHHVLDIIPQQFKTGIILHKTLLTAGIGESFLAELIQDWENKLPTDIKLAYLPK
                     FGMVRLRLTGLGLNKELLAEKMQTEFEKLKTIVQEFLVIDEDLSLEVVVSNLLKQYHQ
                     TVAIAESCTGGAIASSITALPGASSVFKGGIVCYSNYAKNKLLHVPNEILNTVGAVSK
                     ETVEILATNILKNHNSHYALAVSGIMGPTGATETKPIGLVWIAVVSEKNLITKQFNFK
                     YDRERNIQHTTVNALNMLRKLIIEENKNS"
     gene            3154..3708
                     /locus_tag="PLY81_07475"
     CDS             3154..3708
                     /locus_tag="PLY81_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30101.1"
                     /translation="MENYYKLISFEIEPSIDNRRKIINYFTLNGFNIIEENFEKIVCN
                     KKDSFLSSWHFNPLKWSSNVVINLFKNSIEAHFSLDITNINNPKQEIAVWHIFLQNFQ
                     HTLQGKEINTTELNLAIKQSKKTGWLNFCKFIFIYTIVGLIGFYSSKSFGLKYPLFFS
                     LPLIIILTAIFNKKRIEKQLKAFT"
BASE COUNT         1494 a          530 c          587 g         1184 t
ORIGIN      
        1 ccttcaatat ttggatttgc aattgatgaa gaaaaagatt tgcttgcttt tgaaacctat
       61 agtgaaatga cgttgatgaa actaagtaca caaaaaatac tttggagaga caatccactt
      121 gagtcttcca ttaaaatatc ttatcaacct aatggaattg catttggcac taaaagtgaa
      181 ttttatacaa ttgccaataa tttaaataac tgttatttta caacttatac aggtaataaa
      241 ccagtagaaa ttttaaacaa ccatttgtat aaagcagata aatctgccat caacaactta
      301 gcatttgtgt taaaagaaaa aggcttttta cccgaagatt ttgacatgta ttttaacaga
      361 cctgattctg ttacattcga agcaggttca acaaatgcaa cttataatga attattgagt
      421 aaagtggtag aaaaaagaaa aagaaaattc aacacacaaa acattacaga ggttttaagc
      481 aatagtccta cttgtacaat aattaataaa aatgaaattc ccgatgtaac aaaagaaaat
      541 catattctgt tgcaaataaa agcaagttca cctacatctg cagcaattac agctatacat
      601 attttaaaca atggtgtacc tgtttttggc aaagctggtt ttacaaacaa taatcaacca
      661 ataacagaag aacaaaagat tgatgttgtt ttaaccaatg gaattaataa tgttcaagtg
      721 tatgtttcag acaatacagc attggtttct gcatcagaaa gtattatcat cagttcagat
      781 tatatagaag taacagaacc aaaacaacac ttcataggca taggcattaa tcaattctcc
      841 aatccagatt acaatttaaa ctggagtgta aaagacatta gagatttggc actcaagcta
      901 aaatcaaaat ttccaaacat tattatagat acattgtttg atgaaaatgt aaccaaagaa
      961 aaaattttag cagtaaagaa aaaattattg caactgaatg agaatgataa agtaattgta
     1021 tcatacagtg gtcatggtgt attaaacaaa gacttcgatt attttctttc cacgtatagt
     1081 atcaattttg aaaaaccaga agaaaaaggt ttgtcttaca atgatttgga aaatttgtta
     1141 gacagtataa agccacgtca aaaactcatg tttatagatg cctgccatag tggagaagta
     1201 gataaagagg agatagaaaa ggttgaagca tctaagaaag aattagaaaa aaatggagtt
     1261 gtaactaaaa gcactattaa agtaaagcct aaaaaaaaca taggcatggc aaatagtttc
     1321 gaactcatgc aaaacttgtt tgtaaatgta ggcaaaggca caggagcaac tattattagt
     1381 gctgcaggtg gtatgcaata tgcacaggaa agaggcgatt taaagaatgg cgtatttaca
     1441 tacagtgttt tggaagcatt tgataaaaac actacattaa aagtttcaga attaaagaaa
     1501 atagtaggtg aacgtgtaat acaacttacc aatggtttac aaaaaccaac atcaagaaat
     1561 gaagctaata attatgattg ggtagtgtgg taaatggtat tgctgatttt tattaattat
     1621 atttcatttt ttgcagaaat agttttttgt acaaaaattt ttcatgagat atgcatgctt
     1681 aatagtatta ttttttatcc tattttttct aagagcacaa gaaccaactt tagtaactct
     1741 tattgggcat tcagaaacta ttactcttac aaaattttca ccaaatgaac gattgatgct
     1801 tacagcatct gaagatgcct tgtataaact taactttgca actaccggca acaaaaaagt
     1861 tttagtttaa ttattttttt aagtttatga ataaaatttt tgcaacaata attacaatag
     1921 gcgatgaatt attaattggc caggtaatag ataccaatag tgcatttata gctcaggaat
     1981 taaataaaat aggcatttcc gtaaaacaaa gagtttccat agccgatgaa gaagaagcca
     2041 tttttactgc attagataat gcttttattc attcaaatat tattctaata actggtggct
     2101 taggcccaac atccgatgat ataaccaaac caactttatg caaatatttt ggcggaaaaa
     2161 tgaccatgca ccaacctact ctacaacatg taacctattt atttgaacag gtttttaaac
     2221 gcccaatgat tgaacgaaac gtacaacaag caatggtgcc agatgtttgt acagtgctta
     2281 aaaatgaagt tggtactgca ccagcaatgc ttttcaaaaa aaataatacc atttgtattg
     2341 caatgccagg tgtacctttt gaaatgaaat ggattatgca acaccatgtt ttagatatta
     2401 taccacaaca atttaaaaca ggaattattt tacataaaac cttactaaca gctggtattg
     2461 gcgaaagctt tttagcagag ctaattcaag attgggaaaa taaattacca actgatataa
     2521 aattagctta cctgccaaaa tttggtatgg tacgcttaag gttaactgga ttgggcttaa
     2581 acaaagaact gctggcagaa aaaatgcaaa ccgaatttga aaaattaaaa actattgttc
     2641 aagaattttt agtaattgat gaagatctaa gtttagaagt agtagtatct aacctgctca
     2701 aacaatatca ccaaacagta gctattgcag aaagttgtac aggtggtgcc attgcaagtt
     2761 caattactgc attacccggt gctagtagtg tttttaaagg tggcattgtt tgttatagca
     2821 actacgctaa aaataaatta ctacatgtgc caaatgaaat attaaacaca gttggtgctg
     2881 taagtaaaga aacggtagaa attttagcaa ctaatatatt aaaaaatcat aacagccatt
     2941 atgcacttgc agtaagtgga attatgggac caactggtgc aacagaaaca aagcctattg
     3001 gacttgtttg gatagcagta gtatccgaaa aaaatttaat aactaaacaa tttaatttta
     3061 aatacgacag agaaagaaat atacaacaca ccactgtaaa tgccttaaat atgctaagaa
     3121 aattaattat agaagaaaat aaaaatagct gaaatggaaa attattataa acttatctct
     3181 tttgaaatag agccatcaat tgataatcgt agaaaaatca ttaattactt tactttaaat
     3241 ggttttaaca ttattgaaga gaattttgaa aaaattgtat gtaataaaaa agattctttt
     3301 ttaagcagtt ggcatttcaa tcctctaaaa tggagttcaa atgtggttat taatttattt
     3361 aagaatagta ttgaagctca tttttcttta gatataacaa acatcaataa tcctaaacag
     3421 gaaattgcag tttggcatat ttttttacaa aattttcagc atacattaca gggtaaagag
     3481 ataaatacaa ctgaattaaa ccttgcaatt aaacaatcaa aaaaaacagg atggttaaat
     3541 ttttgtaagt ttatttttat ttacactatt gttggtttaa ttggtttcta tagcagtaaa
     3601 tcattcgggt taaaatatcc tttatttttt tctttgcctc ttattattat tcttacagca
     3661 atatttaata aaaaaagaat agaaaaacaa ttaaaagctt ttacttaacc gctcatacat
     3721 ttttaaacat aaatgaaaat aaaaaaagcc attcaacttt taaattaaat ggcttaaaaa
     3781 aatatcaggt ttaat
//
LOCUS       DAOQHP010000221         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_14971_length_3783_cov_2.871245, whole genome shotgun sequence.
ACCESSION   DAOQHP010000221 DAOQHP010000000
VERSION     DAOQHP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14971_length_3783_cov_2.871245"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLY81_07480"
     CDS             complement(<1..251)
                     /locus_tag="PLY81_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30102.1"
                     /translation="MKLNKTQLYLILGVVIVICIPLVAMLFTTEVKWSFFDFVLAAVL
                     LLFVALLCEFLYRKINNIVLKIITCSLVIITCILFWIEIA"
     gene            complement(329..1144)
                     /locus_tag="PLY81_07485"
     CDS             complement(329..1144)
                     /locus_tag="PLY81_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF30103.1"
                     /translation="MPQEKLTDLHSVYTETLTLTSVYLERDVIIDVYFPNKVDKPENL
                     SLLIFNDGQDLLKMPFDEILDELTSQNIIEPLFCIGIHCGEERKREYGTAYSADFKQR
                     GDKAGLYNKFLFDELLPTIRYAYHIPSFKEKSFAGFSLGGLSALDIVWNHANEFTKVG
                     VFSGSLWWRKKSYEDGYDDENDRLMHLQIRKGTFYPWLKFYFQCGALDEVADRNKNGI
                     IDSIDDALDLIVELKIKGYSDKHIQYVLLEDGKHDTTTWANALPNFLKWGWGK"
     gene            1295..2248
                     /locus_tag="PLY81_07490"
     CDS             1295..2248
                     /locus_tag="PLY81_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30104.1"
                     /translation="MRKIGILHGKERSFPEALVAAINSKNIPGIVAEPVRIDKVMQGE
                     PSDYAVIIDRISQDVPFYRAYLKNAALCGTAVINNPFWWSADEKFFNNCLATKIDVPV
                     PKTVILPSRDLPDDTSSESFSNLAYPLDWESIFNYTGFPAYMKPFAGGGWKSVYKLES
                     MEDFFEKHAETKQLVMLLQEEIIFEEYYRCYCIGGKYVRIMPYEPRNPHHLRYVASFT
                     PSAERLKLMEEIVLRINHYLGYDFNTVELAVRNGVPYAIDFCNPAPDADKNSVGEDNF
                     AWVVETASKYAIEKAQSFEPGKDNLTWGKYIQHSAAKRTLY"
     gene            2334..3443
                     /locus_tag="PLY81_07495"
     CDS             2334..3443
                     /locus_tag="PLY81_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNF30105.1"
                     /translation="MANINYRHFTLGVEEEYMVIDPTTRELKSHEQKIVQEGQKLIKD
                     KVKAEMHQAVVEVGTDICYDIDEAFKDVSTLRRTISQIAGDLGLWVGASGTHPFSHWE
                     SQLITEHVRYNEIVNELQEAARSNLIFGLHVHVGMEDRKMAIHIANSARYFLPHVYAL
                     STNSPFWEGRKTGYKSFRTKVFDKFPRTGIPDYFDSIEAYDNYVNLLIKTHCIDNAKK
                     IWWDLRVHPFFNTVEFRICDVPLTVTETIAIAALFQAICAKLYKLRSENLNFIIYQRA
                     LLNENKWRAGRYGIDGKMIDFGKESEVNTRLLIYELLDFVDDVVDDLGSRPFITYIQK
                     MLEQGTGADRQLAVFEETKDLVKVTDYIHDQFLSL"
     gene            3541..>3783
                     /locus_tag="PLY81_07500"
     CDS             3541..>3783
                     /locus_tag="PLY81_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNF30106.1"
                     /translation="MQTFNQINIVECPRDAMQGWKNFIKTEDKIRYINSLLKVGFHTL
                     DFGSFVSPKAIPQMADTKEVIKGLQINNHTKLLAIVA"
BASE COUNT         1392 a          567 c          595 g         1229 t
ORIGIN      
        1 gctatttcta tccaaaataa aatacatgta attataacca agctacatgt aataattttc
       61 agaactatat tatttatttt tctgtataaa aactcacata gtaaagcaac aaaaagtagt
      121 agtacagcgg caagtacaaa atcaaaaaaa ctccacttta cttcagtagt aaataacata
      181 gctactaatg gaatacaaat aactattaca acaccaagta taagatataa ttgagttttg
      241 tttagtttca ttaaaacatt ttttacaata ataagttata aagctaatct aaaataagta
      301 caagaattgt taatcgtttt ttaaaaccct atttgcccca cccccatttt aaaaaatttg
      361 gcaaagcatt agcccaagtg gttgtatcat gttttccatc ttctaataaa acatactgta
      421 tatgcttatc agaatatcct tttattttta attcaacaat taagtctaaa gcatcgtcaa
      481 tgctatctat aatgccattt ttatttctat ccgcaacttc atctaatgcc ccacattgaa
      541 aataaaattt caaccatggg taaaaagtgc cttttcttat ttgcaagtgc attagcctat
      601 cgttttcatc atcataacca tcctcataac ttttttttcg ccaccataaa ctaccactaa
      661 aaacgcccac tttagtaaat tcgtttgcat ggttccatac aatatctaag gcacttaacc
      721 cacctaacga aaacccagca aacgattttt ctttaaaaga tggtatatga taagcatatc
      781 tgatggtagg caataattca tcaaataaaa atttattgta taagcctgcc ttatctcctc
      841 tttgtttaaa atctgcacta tatgcagtac catattctct tttcctttct tcgccacaat
      901 gaattccaat acaaaacaat ggttcaatta tgttttgaga tgttaattcg tctaatattt
      961 catcaaaagg cattttcagt aaatcttgcc catcattaaa aattagtaaa cttaaattct
     1021 ctggtttatc tactttatta ggaaagtaaa catctattat aacatcacgt tctagatata
     1081 cagatgtaag ggttagcgtt tctgtgtaaa cagaatgtaa gtcagttaat ttttcctgag
     1141 gcatatattt tcaaaaataa gggaaagcat taaaaataat taaatgattt tttcacaaaa
     1201 aatttgtttt tcagtctata agttctatat tgaaaaagga aaaataataa cacttattta
     1261 ttcaatcttt tttaataatt taaactatcc ctttatgaga aaaattggta ttttacacgg
     1321 aaaagaaaga agttttccgg aagctttggt tgctgcaatt aacagtaaaa atataccagg
     1381 aattgttgct gagcctgtac gtatagacaa agtaatgcaa ggcgaaccat ctgattatgc
     1441 tgtaataata gatcgtatta gtcaagatgt tcctttttac agagcctatt taaaaaatgc
     1501 agcattatgt ggtactgcag taattaataa ccctttttgg tggagtgcag atgagaaatt
     1561 ttttaataat tgcttggcaa caaaaataga tgttcctgta cctaaaacag ttattctacc
     1621 ttctagagat ttaccagacg atacctctag cgaatctttt agcaatttag cttacccttt
     1681 agactgggaa agtattttta attacactgg ctttcctgct tacatgaaac catttgctgg
     1741 tggtggctgg aagagtgttt ataagttaga aagcatggaa gacttttttg aaaaacatgc
     1801 cgaaacgaaa caattagtaa tgttacttca agaagaaatt atttttgaag aatactatcg
     1861 ttgttattgc ataggaggaa aatatgtacg aataatgcct tacgagccac gcaatcctca
     1921 tcatttacgt tatgttgcaa gttttactcc aagtgcagaa aggttaaaac taatggaaga
     1981 aattgtactt cgtattaacc attatttagg atacgatttt aatacagttg aattagctgt
     2041 tcgtaatggc gtaccttatg caatagattt ttgtaaccct gcaccagatg cagataaaaa
     2101 tagtgttggg gaagataatt ttgcatgggt agtagaaact gcttcaaaat atgcaattga
     2161 aaaagcacag tcgttcgagc ccggaaaaga taatttaact tggggtaaat acatacaaca
     2221 cagtgcagct aaaagaactt tatactaaac caaataattt attttaaata aagaaacgga
     2281 ataaatattt tattaaaaag cagctattat ttacttaaaa cttattcaac taaatggcaa
     2341 atattaatta cagacatttt actttaggag tagaggaaga atacatggta atagacccta
     2401 caacacgtga attaaaaagt catgagcaaa aaatagtaca agaaggtcaa aaactaataa
     2461 aagataaagt aaaagcagaa atgcatcagg ctgttgtaga agttggtaca gacatttgtt
     2521 atgatataga cgaagctttt aaagatgtat ctacactaag aagaacaatt agccaaattg
     2581 caggagattt aggtttatgg gttggtgcaa gtggtacaca ccctttcagt cattgggaaa
     2641 gccaattaat tactgagcat gtacgctata atgaaattgt aaacgaatta caagaagctg
     2701 caagaagtaa tttaattttt gggctgcatg tacacgtagg tatggaagat agaaaaatgg
     2761 ccattcatat tgccaatagt gccagatatt ttttgccaca cgtatatgct ttaagtacca
     2821 attcgccttt ttgggaagga agaaaaacag gatacaaatc ttttagaaca aaagtttttg
     2881 ataaatttcc tcgtacaggt atcccagatt attttgacag cattgaagca tacgataatt
     2941 atgtaaacct tcttataaaa acacattgta tagataatgc taaaaaaatt tggtgggatt
     3001 taagagtgca tccttttttt aataccgttg agtttagaat ttgcgatgtg ccacttactg
     3061 taaccgaaac cattgctatt gctgcattat ttcaggctat ttgtgcaaaa ctatataagc
     3121 taagatcgga aaatttgaat tttattattt atcaaagagc cttattaaat gaaaacaagt
     3181 ggcgagctgg cagatatggt attgatggaa aaatgataga ttttggaaaa gaatctgaag
     3241 taaatacaag gttgctgata tacgagttat tagattttgt ggatgatgta gtagatgatt
     3301 taggtagcag accatttata acttacattc aaaaaatgtt agagcaaggc acaggtgcag
     3361 atagacagtt ggctgttttt gaagaaacaa aagacttagt taaagtaaca gattatattc
     3421 atgaccaatt tttaagtttg taattagttt taccaaaaaa tttaaaacag ttaatatttt
     3481 tattaactgt ttttttatgc aataagtaaa tacttttaca cctttaaaca ggcaaaaaaa
     3541 atgcaaacat ttaatcaaat taatattgtt gaatgtccca gagatgctat gcaaggttgg
     3601 aaaaatttta ttaaaacaga agataaaatt agatacatca acagcttatt aaaagttggg
     3661 tttcatactt tagattttgg aagttttgtt tcaccaaaag caatacctca aatggcagat
     3721 accaaagaag taataaaagg gctacaaata aataaccata caaaactatt agccattgta
     3781 gct
//
LOCUS       DAOQHP010000222         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15055_length_3770_cov_3.405653, whole genome shotgun sequence.
ACCESSION   DAOQHP010000222 DAOQHP010000000
VERSION     DAOQHP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15055_length_3770_cov_3.405653"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="PLY81_07505"
     CDS             complement(<1..1792)
                     /locus_tag="PLY81_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF30107.1"
                     /translation="MQLIKRTLWLLLILTVSFSYAQNTPLPVDPSVKIGQLANGLKYY
                     IKSNKKPEKKVELRLVINAGSILEDDDQQGLAHMVEHMAFNGTTNFKKNDIISYLQSI
                     GVGFGNDLNAYTSFDETVYMLPIPTDKPGNLEKGFQIIEDWAHNVTFLDDDIDGERNI
                     ILEESRLGKGAEDRMFKKVYPILFEGSKYGNRLPIGIESIIKTFKHDAIRRFYRDWYR
                     PNLMAVVVVGDIDIATAEAYVKKHFTGLKNPENERPRTNFDVPAYKANQGIVVTDKEA
                     TMYVSMLNYSAEKHHEATNIDEYKASIIQSLFTTILNQRLREVTQQTNPPFLGASVEF
                     GSYARGFESFQASVYAGAGDVSKSVSALVEEIERAKRFGLTQAELDRAKTNLLTRMER
                     MNNEKDKTESADFVEEYIRNFLTNEPIPGIATEYELHKTLLPTITLADVNAIGNSLKN
                     NSNFVTAVTGPIATDGVTLPKDEELVTIVTSAASKQDIKPYEEKEIAKNLLSKEPKAG
                     KIISSTKNIKLGTTTYVLSNGIKVTVKKTDFKNDQILLSARRFGGLNNYGIADKYNAN
                     FLTSVISSMGIGAFSPTDLRKALTGKTATIN"
     gene            complement(2025..2483)
                     /locus_tag="PLY81_07510"
     CDS             complement(2025..2483)
                     /locus_tag="PLY81_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30108.1"
                     /translation="MLKKYSLLTTLLLVIFIGTSCKKIKSFEYRDVRNIEVKSLGFNK
                     SSLTMNLVYFNPNEFGVDLRKVNCDVFIDNNFFGKYSLDTLMHIKRRSEFALPSRIDI
                     EMKGIYKNLYNVIFNKEIQLNVKGTAKVGKAGIFVNMPFNYNGKHTINFF"
     gene            complement(2538..>3770)
                     /locus_tag="PLY81_07515"
     CDS             complement(2538..>3770)
                     /locus_tag="PLY81_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNF30109.1"
                     /translation="NVAEPVVEMLFPDRSNGRIPIIAVTGTNGKTTTTRLTAHISKSA
                     GKKVGYTTSDGVYIQNQLLMKGDCTGPISSQFVLKDPTVDFAILECARGGILKSGLAF
                     QNCDVAIVTNVAEDHIGLGGINNLEQMAKVKAVVPETVFKHGYAVLNADDDLVFNMIP
                     TLSCNIALFSMNENNPRIVEHCNAGGLATVYENGFISILKGTWKIRVAHVKDIPITFE
                     GKAVHNIANCLPSVMATYLFKSIKIEDIRSGLQTFIAGSNTTPGRLNFFNFKNFTFLA
                     DFAHNPHGLKLLGQFVSKLDYKTKVGVISGTGDRRDEDIRELGAISAEYFDEIIIRCD
                     KNLRGRTAEEIIGLLQEGINTVNPNIPQTVIANENLALEHIYANHKPGALYTIMCDVV
                     AGALDKIKELKDRENKNG"
BASE COUNT         1184 a          638 c          520 g         1428 t
ORIGIN      
        1 gattaattgt tgcagtttta ccagttaatg ctttacgcaa atctgttggc gaaaatgctc
       61 caatacccat agaactaatt actgaggtta agaaatttgc attgtatttg tctgcaatac
      121 cataattatt taaaccacca aaacgtcttg cacttaataa aatttggtcg tttttaaaat
      181 ctgttttctt aacagtaact ttaatgccat tacttaaaac gtacgtagta gtaccaagtt
      241 ttatattttt tgtagaagaa ataattttgc cggctttagg ttctttactt aataaatttt
      301 tggcaatttc tttttcttcg taaggtttaa tatcttgttt agatgctgca ctagttacta
      361 tagttacaag ttcttcatct tttggtagag ttacaccatc tgtagcaatt gggcctgtaa
      421 cggctgtaac aaaattgcta ttatttttta aactatttcc aattgcattt acatcggcca
      481 aagtaattgt tggtaataat gttttatgca attcatattc tgtagcaata ccaggtatag
      541 gttcgttagt taaaaaatta cgaatatatt cctctacaaa atcagcagat tctgttttgt
      601 ctttttcgtt attcattctt tccattctgg ttagcaaatt ggtttttgct ctatctaatt
      661 ctgcttgtgt taaaccaaaa cgttttgctc tttctatttc ttctactaaa gcagaaacag
      721 attttgaaac atcacctgca ccagcataca cagaggcttg aaaagattca aaacctcttg
      781 cataagagcc aaattcaaca gatgcaccta aaaatggtgg gttggtttgt tgggtaactt
      841 ctcttaatct ttgattaaga atagtggtga ataaagattg aataatactt gctttgtact
      901 catctatatt ggttgcttca tgatgttttt cagcactata gttaagcata gaaacataca
      961 tggtagcttc tttatctgta acaacaattc cttgattggc tttataagct ggcacatcaa
     1021 agtttgttct tggcctttcg ttttccggat tttttaagcc tgtaaaatgt ttctttacat
     1081 aggcttcagc tgttgcaata tcaatatcac ctacaacaac aacagccatt aaatttggac
     1141 gataccaatc tctataaaaa cgacgaatag catcgtgttt aaatgtttta attatacttt
     1201 caattccaat tggtaaacga ttaccatatt tactgccttc aaaaagtata ggatacactt
     1261 ttttaaacat tctgtcttct gcacctttgc ctaaacggct ttcttctaaa ataatatttc
     1321 tttccccatc aatatcatca tctaaaaaag taacattgtg tgcccaatct tctataattt
     1381 gaaatccttt ttcaaggttt cctggtttat ctgttggtat gggtaacata taaacagttt
     1441 catcaaaact ggtatatgca ttcaaatcgt taccaaagcc tacaccaata ctttgtaaat
     1501 aggatataat atcattcttt ttaaagttgg tggtaccatt aaacgccata tgttctacca
     1561 tatgtgctaa accttgttgg tcatcatctt ctaaaattga acctgcatta attactaaac
     1621 gcaattcaac ttttttctct ggttttttat tgcttttgat gtagtacttt aagccatttg
     1681 ctaattgacc aattttaaca cttgggtcaa ccggcaatgg agtgttttgt gcatagctaa
     1741 aacttacggt tagtataagt aaaagccaca atgttctttt aataagttgc atattttaaa
     1801 atttattgtt gcgaagtaat gaaatgaaaa agaagtaaac aaaaaagagt ttagattatt
     1861 ttgtggttat ttgcaaagga gctattattt cttatttttt aacttttcta tctatacaaa
     1921 ttatagaaaa agaaagtggt ttttatttta attgtacaat taataaataa gttgtaaaaa
     1981 ttgccaaaac actatattta cttttctatt ttaattcatt taacttaaaa aaagttgata
     2041 gtatgcttgc cgttgtaatt aaaaggcata tttacaaaaa taccagcttt gccaactttt
     2101 gctgttcctt ttacatttag ttgaatttct ttattaaaaa ttacgttgta taagttttta
     2161 tagatgcctt tcatttctat atcaatacgg cttggcaaag caaattcact tcttctttta
     2221 atgtgcatta gggtatctaa agaatatttt ccaaaaaaat tgttgtctat aaatacatca
     2281 caatttactt ttcttaaatc aacaccaaat tcatttgggt taaagtacac caaattcatg
     2341 gttaaactag acttgttaaa ccctaatgat tttacttcaa tatttcttac atctctgtat
     2401 tcaaaacttt ttattttttt acaagatgta ccaataaata taaccaataa taaagtggtt
     2461 agtaaactat attttttaag catttttgta cttaaataat ggaaatagta attataaatg
     2521 cagtaataaa ttaattatca cccattttta ttttctctgt cttttagttc ttttatttta
     2581 tctaaagcac cagccactac atcgcacatt attgtataaa gtgcaccagg tttatggttg
     2641 gcatatatat gttctaatgc taaattttcg ttggcaataa ctgtttgagg aatattaggg
     2701 tttactgtat taataccttc ttgtaaaagg ccaattattt cttcggctgt tctacctctt
     2761 aaatttttgt cacatctaat tattatttca tcaaaatatt cggcagatat tgctccaagt
     2821 tctctaatat cctcgtctct tctgtcacct gttccactaa taacacctac cttggtttta
     2881 taatctagct tacttacaaa ttgccctaaa agctttaacc catgagggtt atgagcaaag
     2941 tcggctagaa atgtaaagtt tttaaagttg aaaaagttta atcgtccagg agttgtatta
     3001 ctacctgcaa taaaagtttg taatccgctt cttatatctt caattttaat tgatttgaat
     3061 aaataagttg ccataacact tggtaagcag ttggcaatat tatgaactgc ttttccttca
     3121 aatgttatag gaatatcttt tacatgtgca actcttattt tccatgtacc ttttaaaata
     3181 cttataaagc cattttcgta aactgttgct aagcctccag cattgcaatg ttccacaatt
     3241 cttggattat tttcattcat gctaaataag gcaatattgc aagagagagt aggaatcata
     3301 ttaaatacta aatcatcgtc tgcatttaat acagcatatc catgtttaaa aactgtttcc
     3361 ggaacaacag cttttacttt agccatttgt tctaaattat ttattccgcc aaggccaata
     3421 tgatcttcag ccacattagt aactattgca acatcgcaat tttgaaatgc taaaccactt
     3481 ttaagaatac cacctcttgc acattctaaa atggcaaaat ctacagtagg atcttttaaa
     3541 acaaattgag aagatattgg gcctgtacaa tctcctttca ttaataattg attttggata
     3601 tacactccat cgctcgttgt ataaccaact tttttgcctg cacttttgct aatatgagca
     3661 gttaaacggg tagttgtagt tttaccattt gtacctgtaa ctgcaataat gggtattctg
     3721 ccattgcttc tatctggaaa caacatttca acaacaggtt ctgcaacatt
//
LOCUS       DAOQHP010000223         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15120_length_3760_cov_3.193252, whole genome shotgun sequence.
ACCESSION   DAOQHP010000223 DAOQHP010000000
VERSION     DAOQHP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15120_length_3760_cov_3.193252"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLY81_07520"
     CDS             <1..294
                     /locus_tag="PLY81_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF30110.1"
                     /translation="RTSQIGLKKAIGAKSKIILTEFLIESAFLCMIGGLIGLAFVWLL
                     TKALGGVLPFPIFIAPNIILLAFNICVVLGVLSGIIPATIAAKMNPVVAIRTK"
     gene            324..1337
                     /gene="tsaD"
                     /locus_tag="PLY81_07525"
     CDS             324..1337
                     /gene="tsaD"
                     /locus_tag="PLY81_07525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNF30111.1"
                     /translation="MSIYILAIESSCDDTSASVCKDGEILSNYIANQAIHQQYGGVVP
                     ELASRAHLQNIVPVVDIAIKEAAKKIKTNFNLNQLNAIAFTQAPGLIGSLLVGTQFAK
                     SLALALNIPLIAVHHMQAHVLANLIEEEKPSFPFLCLTVSGGHTQIVIAHSALHLEVI
                     GETIDDAAGEAFDKTAKLLGLPYPGGPLIDKYAQLGNANAFKFAEPNIPDLNFSFSGL
                     KTSVLYFLQKQDKQFIEDNLYNICASVQQTIINILMKKLKKAVQQTNIKQICLAGGVS
                     ANSGLRNALIKTGQQNNWKTFIPKFEYCTDNAAMIAITGYYKFINQQFTELDVSPTAK
                     ASW"
     gene            1478..1831
                     /gene="rpsF"
                     /locus_tag="PLY81_07530"
     CDS             1478..1831
                     /gene="rpsF"
                     /locus_tag="PLY81_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNF30112.1"
                     /translation="MNNYELMVIFTPVLSEEEFKNAQKKYAEMVTENGGTIVHTNAWG
                     LKSLAYPIQKKTTGIYWVLEYKVDASFNEKLKVNLLRDEQVMRHLITKLDKYAVEYNA
                     VKKSGVGFNNQKVEA"
     gene            1833..2096
                     /gene="rpsR"
                     /locus_tag="PLY81_07535"
     CDS             1833..2096
                     /gene="rpsR"
                     /locus_tag="PLY81_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNF30113.1"
                     /translation="MAKNEIKYLTAIKQEKPKKKYCRFKKYGIKYVDYKDIEFLKRFI
                     NEQGKMLPRRLSGNSLKYQRKVSDAVKKARQMALLPYVTDLLK"
     gene            2195..2644
                     /gene="rplI"
                     /locus_tag="PLY81_07540"
     CDS             2195..2644
                     /gene="rplI"
                     /locus_tag="PLY81_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNF30114.1"
                     /translation="MEVILIQDVDNLGGKNELVKVKNGYARNFLIPQKFAVEANSSNL
                     KQLNERLKVAAKKEAALLAEITKVVETLKASPVKITAKTGTSGKIFGSVTSLQIARAI
                     REQKGYEIDRKRISINDEIKELGTYKASFDFGNSNTAEIDFEVVSEA"
     gene            2867..>3760
                     /locus_tag="PLY81_07545"
     CDS             2867..>3760
                     /locus_tag="PLY81_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF30115.1"
                     /translation="MNFTTSELTQQVAQTAKDFALQHIKPHLMEWDESQEFPIHIFKE
                     LGKLGMMGVLVPEQYGGAGLSYFEYNAIIQEISKVCGSIGLSVAAHNSLCTNHILTFG
                     TEEQKQKYLPKLANAEFIGAWGLTEPNTGSDAGNMKTVAVQDGNDWILNGTKSWITHG
                     KSGDVTVVICRTGEPRTSGNSTAFIVERGTKGFMAGKKENKLGMRASETAEMIFDNCR
                     ISDAQRLGNVGDGFRQAMKILDGGRISIAALSIGIAKGAYEAALQYSKERQQFDQPIA
                     NFQGISFKLADMATEIMASDLL"
BASE COUNT         1405 a          544 c          655 g         1156 t
ORIGIN      
        1 agaacaagcc aaataggatt aaagaaagca ataggtgcaa aaagcaaaat aatacttaca
       61 gaatttttaa tagaaagtgc ttttttatgt atgattggtg gattaatagg tttggctttt
      121 gtttggctgc taaccaaagc actaggaggt gttctcccct tccctatttt tattgctcca
      181 aatataatat tattggcatt taatatttgc gttgtattag gtgttttatc tggtattata
      241 cctgctacta ttgcagctaa aatgaatcct gtagttgcta taagaaccaa ataaattaat
      301 agttggatta aaaaataata ctcttgtcaa tttatattct ggccatagaa tcttcctgcg
      361 atgatacttc tgcctctgtt tgtaaagatg gagaaatatt atcaaactac attgccaacc
      421 aagctatcca tcagcaatac ggaggtgtag tgcccgaatt agcaagtaga gcccatttac
      481 aaaatattgt tcctgttgta gatattgcaa ttaaagaagc agccaaaaaa attaaaacta
      541 acttcaacct aaaccaactt aatgcaatag cttttacaca agctcctggt ttaattggta
      601 gcttattggt tggtacacaa tttgcaaaat ctttagcact tgctttaaac ataccattaa
      661 tagcagtaca ccacatgcag gctcatgtat tagccaattt aattgaagaa gaaaaaccca
      721 gtttcccttt tttatgttta actgttagtg gtggtcatac acaaattgta attgcacact
      781 cagctttaca tttagaagtt ataggagaaa ctattgatga tgcagccgga gaggcttttg
      841 ataaaacagc taaattatta ggcttacctt atccaggagg tccactaatt gataaatatg
      901 ctcagctagg taatgcaaat gcttttaagt ttgccgaacc aaatatacct gacttaaatt
      961 tttcattcag cggattaaaa acttccgttt tatattttct tcaaaagcag gataaacaat
     1021 ttattgaaga taatttatac aatatttgtg cttctgttca gcaaaccatt ataaatattt
     1081 taatgaaaaa gctaaaaaaa gctgtgcagc aaaccaatat aaaacaaata tgcttagcag
     1141 gtggagtaag tgctaatagc ggtttaagaa atgctttaat aaaaacaggg caacaaaata
     1201 actggaaaac atttattcca aaatttgaat attgtaccga taatgctgca atgattgcta
     1261 ttacaggtta ctataagttt attaaccaac agtttactga actagatgta agcccaacag
     1321 ctaaagcaag ttggtaaaag ttattttaac ttcaatttgt ataaataaac taaaactcct
     1381 acttttgcag ccccaattct gttttggcag aatctattgt acaaactgta gaatagtcca
     1441 atgggttaaa accaattatt aaacaaaaaa aacagtaatg aacaattacg aactaatggt
     1501 aattttcact cctgtacttt ctgaagagga atttaaaaat gctcagaaaa agtacgctga
     1561 aatggttacc gaaaatggtg gtacaattgt acacacaaat gcatgggggt taaagtcttt
     1621 agcctatcca attcaaaaga aaactactgg tatttattgg gtgttagagt acaaagtaga
     1681 tgcttctttc aacgaaaaat taaaagttaa tttattacgc gatgagcaag taatgagaca
     1741 tttaattaca aaactcgata aatacgccgt tgagtacaat gcagtgaaaa aaagtggtgt
     1801 aggttttaac aatcaaaaag tggaggcata atatggcaaa gaatgaaata aaatacctta
     1861 ctgccattaa gcaggaaaaa cctaaaaaga aatattgccg ttttaaaaaa tacggaataa
     1921 aatatgttga ctataaagac atagagtttt tgaaaaggtt tattaatgaa caaggtaaaa
     1981 tgttacctcg tcgtttaagt ggtaactctt taaaatatca acgtaaagta agtgatgcag
     2041 taaaaaaagc tcgtcaaatg gcattgttac cttatgtaac cgatttattg aaataaatta
     2101 caaatacaat tagtaaccat tccctaattc tttaaataaa gatgacagtc taaatcaata
     2161 attgattggg ctctgggaac taaaacaaat aaaaatggaa gtaatattaa tacaagatgt
     2221 agataattta ggaggcaaaa atgagctagt aaaagtaaaa aatggttatg ctcgtaactt
     2281 cctaatacct caaaaatttg ctgttgaggc taattcatct aatcttaaac aattaaacga
     2341 acgtttaaaa gtagctgcaa aaaaagaagc tgctttatta gccgaaatta caaaagtggt
     2401 tgaaactctt aaagcttcgc ctgtaaaaat tactgctaaa actggaacaa gtggtaaaat
     2461 atttggtagt gttacttctt tacaaattgc tagagcaatt cgtgagcaaa aaggctatga
     2521 aatcgatcgt aaaagaatat caattaacga tgaaattaaa gaattaggta cctacaaagc
     2581 aagttttgat tttggtaata gcaatactgc cgaaattgat tttgaggttg ttagcgaagc
     2641 ttaattatat aaaatatcta ccctataaaa ataccttaca aattgtaggg tattttttat
     2701 ttagttagtt tataccatat aatcttttct tcaataattt ggctatttta aaataaaata
     2761 tatttttgca aagctaacaa acgttagtaa ggcttttaac gattgattct cattactagt
     2821 cctgtagcaa tgcaggactt atttttaatt caacatttaa ctttatatga attttacaac
     2881 atctgaactt acacaacaag tagctcaaac agcaaaagat tttgcactac aacatataaa
     2941 accccattta atggaatggg atgaaagtca ggaatttcct atacatattt ttaaagaatt
     3001 aggaaaatta ggtatgatgg gagttttagt gccagaacaa tatggaggtg ctggtttaag
     3061 ctactttgaa tacaatgcca ttattcaaga aatatccaaa gtttgtggct caattggctt
     3121 aagtgttgca gcacacaact ctctttgtac taaccatatt ttaacttttg gtacagaaga
     3181 gcaaaaacaa aaatatttac ccaaattagc aaatgcagag tttattggtg cttggggctt
     3241 aacagagcca aacacaggca gcgatgcagg aaatatgaaa acagttgctg tacaagatgg
     3301 taatgattgg attttgaatg gtactaaaag ttggataaca catggaaaaa gtggcgatgt
     3361 tactgttgtt atttgtagaa caggtgaacc aagaacaagt ggtaatagta cagcatttat
     3421 tgtagaaaga ggaacaaaag gttttatggc cgggaaaaaa gaaaataagt taggaatgag
     3481 agcaagtgaa actgctgaaa tgatttttga taattgtaga attagcgatg cacaacgatt
     3541 aggaaatgtt ggtgatggtt ttagacaagc catgaaaatt ttagatggag gaagaatttc
     3601 tattgctgca ctatctatag gtattgctaa aggagcttat gaagcagctt tacaatattc
     3661 aaaagaacgc caacaattcg atcagcccat tgctaacttt caaggaatta gttttaaatt
     3721 ggcagatatg gctacagaaa ttatggcaag cgatttatta
//
LOCUS       DAOQHP010000224         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15200_length_3749_cov_3.718462, whole genome shotgun sequence.
ACCESSION   DAOQHP010000224 DAOQHP010000000
VERSION     DAOQHP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15200_length_3749_cov_3.718462"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="ruvB"
                     /locus_tag="PLY81_07550"
     CDS             complement(<1..1002)
                     /gene="ruvB"
                     /locus_tag="PLY81_07550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNF30116.1"
                     /translation="MSNPNLNKEGEQLSAAEREFENSIRPTIIDDFSGQPQLIENLII
                     FIKAAKIRGEALDHILFHGPPGLGKTTLSRIVANELGVNIKETSGPVIEKPGDLAGLL
                     TNLQPNDVLFIDEIHRLSTVVEEYLYAAMEDFRIDIMIDSGPNARSIQLNLNPFTLVG
                     ATTRSGLLTAPLLSRFGIKSRLEYYNAEVLKKIIERSSAILQTQISKDAAAEIARRSR
                     GTPRIANGLLRRVRDFAQVLNDGKIDIGITQHALKALNVDEHGLDEMDNRILQAIIEK
                     FKGGPVGITTIATAVGEEPGTIEEVYEPFLIQEGFLQRTPRGREVTLKAYEHLGKKPL
                     "
     gene            complement(1077..1736)
                     /locus_tag="PLY81_07555"
     CDS             complement(1077..1736)
                     /locus_tag="PLY81_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30117.1"
                     /translation="MAIQQQTYQQANNSIVLIVAKAISYIFHPLFIPTYIFWFLTIYF
                     PFEFPGITQKLLGLRIFSVFWMTAFFPALSVFLLWRLKFIDNVFLRTQKERIIPFFIT
                     MFFYWWMYYLSRNFTDQPIALKFFFFGIFISTSVGVVINNYIKISLHGIAMGGALMAI
                     ILISNYYQINLSIPICVAIMLTGMVASSRFIAGNHTDAEMYIGIVVGIVCQLFGYWFV
                     L"
     gene            complement(1748..3235)
                     /gene="rpoN"
                     /locus_tag="PLY81_07560"
     CDS             complement(1748..3235)
                     /gene="rpoN"
                     /locus_tag="PLY81_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNF30118.1"
                     /translation="MSLSQSLQQKLLQKLSPQQIQLMKLLQVPTANLEDRIKEELEEN
                     PALEQVDDAYDNDYNDEFKDEFESNNEEEVLDGSEDEYENIDISEYVSDSDDDIADYK
                     TRDENYGDTEENKIIPIKNEQSFYELLVNQLGLLQLDDRMYKVAEQIVGSLDDDGYLR
                     RELSSIVDDLAFRQNIITNEQEVAELITQIQQFDPPGIAAKDLQECLLLQLKREITED
                     DYVKIAIKILQKYFDEFTKKHFDKIQKGLGINEVELKEAIQQIVKLNPKPGGNIGEIN
                     KAENYIVPDFFILNNAGILELTLNNKNAPDLRISEGYRDMLKDYEKGSKKDKKQKEAV
                     LFIKQKIDSAKWFIEMIQQRQRTLFNTMYAIMKHQENFFLTGDETELKPMILKDIAEV
                     TLQDISTVSRVANSKFVQTEFGNYRLKFFFSESLSTDSGEEVSTREVKKILSDIINAE
                     DKNKPLADEVLTDLLNEKGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            3393..>3749
                     /locus_tag="PLY81_07565"
     CDS             3393..>3749
                     /locus_tag="PLY81_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30119.1"
                     /translation="MLTKATIEKFFIAEKQIGFFLLIVGLIALTIAVYALFYLKLQEL
                     KWPTIIICLLSIVMLFVGYQQVANATKSRISIIYAFDMNPELIKTQELPRVEKKIITI
                     QVLKYISVLANGEIDLQ"
BASE COUNT         1157 a          634 c          505 g         1453 t
ORIGIN      
        1 aagtggtttt tttcctaagt gttcgtaagc ttttaaagtt acctctctac ctcttggtgt
       61 tctttgtaaa aaaccctctt gtattaagaa tggttcatat acttcttcaa ttgttccagg
      121 ctcttcgcct actgcagttg caatggtagt aatacctaca gggccacctt taaatttttc
      181 tattatagct tgtaaaattc tgttgtccat ttcatctaag ccatgttcat ctacattcaa
      241 ggcttttaaa gcatgttgtg taatgccaat atcaatttta ccatcattta aaacctgtgc
      301 aaaatctctt actcttctta ataagccatt ggcaatacgt ggtgtacctc tgcttcttct
      361 tgcaatttct gctgcagcat ctttagaaat ttgggtttgt aaaattgcac tacttctttc
      421 aataattttt ttcaggactt ctgcattata atattctaat ctgcttttta ttccaaatct
      481 gctgagtaat ggtgctgtta ataaaccact tcttgttgtt gcacctacta aagtaaatgg
      541 gtttaggttt aattgaatac ttcttgcatt tggtccacta tctatcatta tatcaattct
      601 aaaatcttcc atagcagcat ataaatattc ttcaacaaca gtacttaatc tatgaatttc
      661 atcaataaat aaaacatcgt ttggttgtaa attggttaat aagcctgcga ggtcgccagg
      721 tttttctata actggcccac tagtttcttt tatatttaca cctaattcat tagcaacaat
      781 tctgcttaat gttgttttac ctaaccctgg tggtccgtga aataaaatat ggtctaatgc
      841 ttcgccacga attttggcag ctttaataaa aatgattaaa ttttctatta gttgaggttg
      901 tccactaaag tcatcaataa tggtgggcct aatactgttt tcgaattcac gttcagccgc
      961 cgaaagttgc tccccttctt tattcaaatt aggattactc atattatttc aaaactacaa
     1021 caatgaaagg gtatgtaaaa attaaaacgt aattaattgt ataagcaatg tatattttac
     1081 agtacaaacc aataaccaaa aagttggcaa actataccta ccacaatacc aatatacatt
     1141 tctgcatcgg tgtgattgcc agcaataaaa cggctacttg caaccatgcc agtaagcata
     1201 atagctacac aaataggaat acttaagtta atttgataat aatttgaaat taaaataata
     1261 gccattaatg caccacccat ggctatgcca tgcaaactaa tttttatgta attatttatt
     1321 actacaccta ccgaagtgga gataaatata ccaaagaaaa aaaactttaa tgcaataggc
     1381 tgatctgtaa aatttctgct taggtaatac atccaccaat agaaaaacat ggtgataaaa
     1441 aaaggtatta ttctttcttt ttgtgtacgt aagaaaacgt tatcgatgaa ctttaaacgc
     1501 cacaacaaaa aaacagataa agctgggaaa aaagctgtca tccaaaatac actaaatatt
     1561 cttaaaccta atagtttttg tgtaattcca ggaaactcga atgggaagta aatggttaaa
     1621 aaccaaaaaa tataagttgg tataaataat ggatgaaaaa tatagctgat tgcttttgct
     1681 actataagta caatactatt gtttgcttgt tgataggttt gttgctgaat tgccacggta
     1741 aaaaatttta taattctttt cttaatctag ctacgggtat gtttaattgt tctcggtatt
     1801 ttgccactgt tcttcttgca atattatagc ctttctcgtt caacaagtct gtaagaactt
     1861 cgtctgccaa aggtttattt ttatcttcgg cattaataat atcgctcaat atttttttaa
     1921 cttctcttgt acttacttct tcaccagaat ctgtacttag actttcgcta aagaaaaatt
     1981 ttaatctgta attaccaaat tctgtttgta caaacttgct attggcaaca cggcttacag
     2041 tgctaatatc ttgtaaggtt acttctgcaa tatcttttaa aatcattggt tttaattctg
     2101 tttcatcgcc tgtaagaaaa aagttttctt gatgtttcat aatggcatac atggtgttaa
     2161 ataatgttct ttgcctttgc tgaatcattt caataaacca ttttgcacta tctatttttt
     2221 gtttaataaa taatactgct tctttttgtt ttttatcttt tttacttcct ttttcatagt
     2281 ccttcagcat atctctgtaa ccttcactaa ttcttaaatc tggtgcattt ttattgttta
     2341 aggtaagttc taaaatgcca gcattattca aaataaaaaa gtctggtact atataatttt
     2401 ctgctttgtt tatttcgcca atattgccac ctggttttgg atttagtttt acaatttgtt
     2461 gaatagcttc tttaagttcc acttcattaa tgcctaaccc tttttgaatt ttatcgaaat
     2521 gctttttagt aaattcatca aaatattttt gtaaaatttt gatggcaatt tttacatagt
     2581 catcttcggt tatttccctt tttaattgca ataacaaaca ttcttgtaaa tcttttgcag
     2641 caattcctgg ggggtcaaac tgttgtattt gggttattag ttcagcaact tcttgttcat
     2701 ttgttattat gttttgtcta aaagcaaggt catctacaat gctgcttaac tctcttctta
     2761 agtatccatc atcatctaaa ctgcctacaa tttgttctgc cactttatac attctatcat
     2821 ccaattgtaa caagccaagt tgattaacta ataattcata aaagctttgt tcgttcttaa
     2881 ttggtattat tttattttct tctgtgtcgc catagttttc atctcttgtt ttataatctg
     2941 caatatcatc atcgctatca cttacatatt cactaatgtc tatgttttca tattcatctt
     3001 cgctgccatc taaaacttcc tcctcattat tgctttcaaa ttcatcttta aattcatcgt
     3061 tatagtcgtt atcatatgca tcatcaactt gttctaaagc aggattttct tctaactctt
     3121 ctttaattct atcttctaaa tttgcagtag gcacttgcaa caatttcatt aactgaattt
     3181 gttgagggga taatttttgt aataattttt gctgtaaaga ctggcttaaa ctcattgtaa
     3241 ataactttta taaatatatc cttttcattg gctgtaaagt taattgtaaa ctatactatg
     3301 aaagccattt tttgtaattg tttttttgaa aaaaaatggt ttaattgtcc tttcaaataa
     3361 agtttatctt cacactttaa aattatttac taatgcttac taaagcaact attgaaaaat
     3421 tttttattgc agaaaaacaa attggatttt ttttattaat tgttggtctt attgctttaa
     3481 caattgcagt atatgcctta ttttatttga agttacaaga attgaaatgg cctaccatta
     3541 taatttgcct actaagtatt gttatgctat ttgtaggcta tcaacaagtt gccaatgcta
     3601 ctaaaagtag aattagtatt atttatgctt ttgatatgaa tcctgaatta attaaaacac
     3661 aagaattgcc aagagttgaa aaaaaaataa taactattca ggtattaaag tatatttctg
     3721 ttttagctaa cggcgaaatc gatcttcag
//
LOCUS       DAOQHP010000225         3745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15228_length_3745_cov_3.902981, whole genome shotgun sequence.
ACCESSION   DAOQHP010000225 DAOQHP010000000
VERSION     DAOQHP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15228_length_3745_cov_3.902981"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /gene="ligA"
                     /locus_tag="PLY81_07570"
     CDS             <1..1925
                     /gene="ligA"
                     /locus_tag="PLY81_07570"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNF30120.1"
                     /translation="KLKVGKKQDRITKAEKENPEIITKDSPTQRVGSSLNNGFETVQH
                     LVPMLSLENSYNETDLLDFDRKAKEASGLQEIEYCVEPKLDGASISLIYENDLLTRAT
                     TRGNGVEGDDITTNIKQIRSIPLSAPFSKYGIRQIEIRGEVMMTKEAFQKYNEKLTQQ
                     GLATVANPRNTAAGSLRIKDPKEVAQRNLDAFLYHISYITQNNTHKVNTHSQQLNLLW
                     NCGFRSTENEKFVAKNINEVIEYVTSFEQKRDDLPYEIDGMVIKVNSLLLQEQLGMTS
                     HHPRWAIAYKFKARQATTVLNAVEFQVGRTGAITPVAKLEPVFIGGVTVSSISVHNVE
                     YIKEKDLKIGDTVLIERAGDVIPQIVKSITTARKGNEHEIVFPENCPVCKNQLFKEEG
                     EAVWRCINIECEAQVVEKIIHFASKDAMDIKGLGDAIVRKFYEKNLLKDIPTIYKLNL
                     TNIGEIEGFGKKSIDNLQAAIASSKQQPLNRLIYGLGIRFVGETTAKTLANSVEHLLE
                     FKNKTTDDLQTLEDVGTKVAKSIHHFFSNESNINMILELEQLGLQLKNTKKQTINIGE
                     LAGQTFLFTGTLSKLKRNQAEALAEQHGGIIVSGVSAKLNYLIVGDDAGSKLEKAKKI
                     NTIKILNEEEFLQMIG"
     gene            complement(1937..2365)
                     /locus_tag="PLY81_07575"
     CDS             complement(1937..2365)
                     /locus_tag="PLY81_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNF30121.1"
                     /translation="MKNLNLVIDTNIFLVSIAKNFRYRWIYDALLNGKFNLLISTEIL
                     LEYEELFQIKYGVKYSSDKFVEFLLNKNVLLISPSFRWQTIYPDLDDNKFVDCAIAGN
                     ADFIISNDADFKILKTIDFPKVNVLTLDEFETSFKEYFIS"
     gene            complement(2352..2579)
                     /locus_tag="PLY81_07580"
     CDS             complement(2352..2579)
                     /locus_tag="PLY81_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30122.1"
                     /translation="MNTPNVQIPPSTKFSNVQLELLNLFAHNVSDEELIELKDVLGKR
                     FLSKLSNDVNKAIEEKNITNNDLEKWLNEKS"
     gene            2739..2930
                     /locus_tag="PLY81_07585"
     CDS             2739..2930
                     /locus_tag="PLY81_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30123.1"
                     /translation="MERKIKIFKTFEEQEAYTLQQQGKTTPLERFRNLFYMQQLSKKF
                     NSVKNSTRKITVHHGYFTS"
     gene            2911..3396
                     /locus_tag="PLY81_07590"
     CDS             2911..3396
                     /locus_tag="PLY81_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30124.1"
                     /translation="MDILHPEFIVFLSCAAKNNLRYLLIGGYAVNYYGYNRHTHDLDV
                     WLAPTNENKLAFIQTLLCMQYSENEVALLYEEDFTKPFKANIGTPTADIDVLTFVHSK
                     ISFDDAEKNKVIFAIEKDVTMNIVPYDFLRDMKLFAARNKDLLDVSELEKLRKNKKKN
                     K"
     gene            3547..>3745
                     /locus_tag="PLY81_07595"
     CDS             3547..>3745
                     /locus_tag="PLY81_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HNF30125.1"
                     /translation="MLQKNTLQFLSSLKNNNNKIWFDANRGWYDETKADFINLNEAII
                     KKLKKKDTTLEPLIAKNCIFRI"
BASE COUNT         1452 a          511 c          631 g         1151 t
ORIGIN      
        1 aaaaattaaa agtgggaaaa aaacaagata gaataacaaa agcagaaaaa gaaaatccag
       61 agataattac caaagattct cctacacaaa gagttggtag tagtttaaac aatggctttg
      121 aaacagtaca acatttagtg ccaatgctaa gtttggaaaa cagttataac gaaacagatt
      181 tactagactt tgatagaaag gcaaaagaag caagtggttt acaagaaatt gaatattgcg
      241 tagagcctaa attagatgga gctagtatta gtttaattta tgaaaatgat ttattaacga
      301 gggctacaac tagaggcaat ggtgtagagg gtgatgatat tacaaccaat ataaaacaaa
      361 ttcgttccat tcctctatct gcacctttta gtaaatatgg tatcaggcaa attgaaataa
      421 gaggtgaagt gatgatgacc aaagaggcat ttcaaaaata taatgaaaag cttacgcagc
      481 aaggtttagc aacagttgca aacccaagaa acactgctgc tggaagttta agaattaaag
      541 atcctaaaga agttgctcaa agaaacttag atgccttttt atatcatata agttatatta
      601 ctcaaaataa tacccacaag gtaaatactc attctcaaca gttaaactta ttatggaact
      661 gtgggtttcg ttcaaccgaa aatgaaaaat ttgttgcaaa aaatattaat gaagtaatag
      721 aatatgtaac ctcctttgaa caaaaaagag acgatttgcc ttatgagatt gatggaatgg
      781 ttataaaagt taatagctta ttattacaag agcagcttgg tatgacaagc catcacccac
      841 gctgggcaat tgcttataaa tttaaagcaa ggcaggcaac aactgtttta aatgcagtag
      901 agtttcaggt tgggcgaaca ggagcaataa cacctgttgc aaaactagag cctgtgttta
      961 ttggtggtgt tacagtttct agcatttcag tacacaacgt agagtatatt aaagaaaaag
     1021 atttaaaaat tggagatacc gttttaatag aacgtgcagg tgatgttatt ccacaaattg
     1081 taaaaagcat aaccacagca agaaaaggca atgagcatga aatagttttt ccagaaaatt
     1141 gccccgtttg taaaaatcaa ttatttaaag aagaagggga agccgtttgg cgttgcatta
     1201 atattgaatg tgaagcacag gtggtagaaa aaattattca ctttgccagt aaagatgcaa
     1261 tggatattaa aggtctggga gacgctattg tacgtaagtt ttatgaaaaa aatttattaa
     1321 aagacatccc aactatctac aagctaaact taaccaatat tggcgaaata gaaggttttg
     1381 gcaaaaaatc tatagataat ttacaagctg ctattgcaag tagcaaacaa caaccattaa
     1441 acagattaat ttatggttta ggaataagat ttgttggcga aacaacagca aaaactttag
     1501 ccaacagtgt agagcatttg ttagaattta aaaacaaaac aaccgacgat ttacaaacac
     1561 ttgaagatgt aggaacaaaa gttgctaaaa gtattcatca tttttttagt aatgaaagta
     1621 atataaatat gattcttgaa ttagagcaat tgggtttaca attaaaaaat actaaaaaac
     1681 aaactattaa tattggcgaa ctggctgggc aaacattttt atttaccgga accctatcta
     1741 aactaaaacg taaccaggca gaggctttgg ctgaacaaca tggtggcata attgtaagtg
     1801 gtgttagtgc aaaattaaat tacttaattg ttggtgacga cgcaggaagt aaattagaaa
     1861 aagcaaaaaa aataaatact ataaagattt taaatgaaga agagtttttg cagatgatag
     1921 ggtaaagaat gattgattag gaaataaaat attctttaaa tgaagtttca aactcatcaa
     1981 gtgtcagcac atttactttg gggaaatcta ttgtttttaa tattttgaaa tcagcatcat
     2041 tacttattat aaaatctgca tttcctgcta ttgcacaatc tacaaatttg ttatcatcca
     2101 aatcaggata tattgtttgc caacgaaaag atggagaaat taacagcacg tttttattta
     2161 ataaaaactc tacaaacttg tcgcttgaat attttactcc atatttaatt tgaaaaagtt
     2221 cttcgtattc aagtaaaatt tctgtactga taagtaaatt aaatttccca ttcagcaaag
     2281 cgtcatatat ccacctgtaa cgaaagtttt ttgcaatgga aacaagaaaa atatttgtat
     2341 caattactaa attaagattt ttcattcagc catttttcta aatcattatt ggtaatgttt
     2401 ttttcttcta tggctttatt tacatcattt gaaagtttgc tcaaaaaacg tttgcccaaa
     2461 acatctttta attcaattaa ttcttcatcg cttacattat gagcaaaaag gtttagcaat
     2521 tccaactgca cattgctgaa ttttgttgat ggcggtattt gaacattagg agtattcatt
     2581 gtagtaatat ttggtataaa ggtacagatt ttatttttcc ctttacgtaa acaaaaattg
     2641 ccttaggcaa tagaataaac ctgcaaggct ataagctttg ctgagctggg tttttacaaa
     2701 aaagcagtaa taagctaata tttttataaa tttggaatgt ggaaaggaaa ataaaaatat
     2761 ttaaaacatt tgaagaacaa gaagcataca ctttgcagca gcagggcaaa accactccat
     2821 tagaacggtt tagaaatctt ttttatatgc aacaactttc taagaaattt aattctgtaa
     2881 aaaactcaac aagaaaaatt acagtacatc atggatattt tacatcctga atttattgtg
     2941 tttctttcct gtgctgcaaa aaataatttg cgttatttac ttattggtgg ttatgctgta
     3001 aactattatg gatataacag acatacccac gatttagatg tttggcttgc acctaccaat
     3061 gaaaataaac ttgcttttat acaaaccctg ctttgtatgc aatattcaga aaatgaagtt
     3121 gctctattat atgaagaaga ttttactaag ccatttaagg cgaatatagg taccccaaca
     3181 gcagatattg atgttcttac ttttgttcat tcaaaaattt cttttgatga cgctgaaaaa
     3241 aacaaagtca tctttgcaat tgaaaaggat gttacaatga acattgttcc ttatgatttt
     3301 ttaagagata tgaaattatt tgcagcaagg aataaagatt tgttggatgt ttctgaactt
     3361 gaaaagttga gaaagaataa aaaaaagaat aaataacaaa tcatccttgt tagtgcaaaa
     3421 ttaaattact taattgttgg cgaagacgca ggaaataaat tagaaaaagc aaaaaaaatc
     3481 aatactatta agattttaaa tgaagacgaa tttatacaat tagtggactg ataaaatatg
     3541 gaatctatgc ttcaaaaaaa cacactacag tttttgagtt ctttaaaaaa taataataat
     3601 aaaatttggt ttgatgctaa cagaggttgg tatgatgaaa ccaaagcaga ttttattaac
     3661 ctcaatgagg ctataattaa aaagctaaaa aagaaagata caaccctcga gcccttaatt
     3721 gctaaaaatt gtatttttag aatta
//
LOCUS       DAOQHP010000226         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15596_length_3694_cov_3.162957, whole genome shotgun sequence.
ACCESSION   DAOQHP010000226 DAOQHP010000000
VERSION     DAOQHP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15596_length_3694_cov_3.162957"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..326
                     /locus_tag="PLY81_07600"
     CDS             123..326
                     /locus_tag="PLY81_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30126.1"
                     /translation="MYNKKNNILIKYAGMATQLLVGLGLTTWFGNWIDKKNIFKKPVF
                     TWILPLIFVIGFLIKLIKDLNKK"
     gene            326..733
                     /locus_tag="PLY81_07605"
     CDS             326..733
                     /locus_tag="PLY81_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30127.1"
                     /translation="MFQQSKFGKLIMPFFALFTLVNIVFITWAEKWDAIKLNHTVIIF
                     GNALLFLLSIFSIWMHLKAVNHKNPSVMVRGVLGSTFVKIILIATSILLYLYFTPKEH
                     QSKLAVIAVMVLYLIYVFIEVRVVVRLNKKKVK"
     gene            733..1356
                     /locus_tag="PLY81_07610"
     CDS             733..1356
                     /locus_tag="PLY81_07610"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HNF30128.1"
                     /translation="MPIVEKPLNSLAHFLPDESLDIVLQFIQQYKIHLLVTQKRKTIL
                     GNYKHAGLQSNHKISVNGNLNKYEFLITFLHELAHLLTYEQFKNKVEPHGKEWKFFYS
                     KLLTEFVQLKIFPNDIVIALQKSILNPAATANGETDLLRVLRKYDTANNPQLQMIENI
                     PTHSKFSIENGRIFIKGQKRRKRIECIEISTNKIYLFNPLVEVKLFE"
     gene            1404..1817
                     /locus_tag="PLY81_07615"
     CDS             1404..1817
                     /locus_tag="PLY81_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNF30129.1"
                     /translation="MKKAKPFIALVILVVVAIFLRNCVSNKSNKKEVDENVKQRGLNR
                     NPSNINYTKHAKCRMDCRHIDEKEVVYILENGKINYSKSDLKGKDCNKKYAVEGISKD
                     KQKLRIVFAPCNNEVTVITCIDLDTEWECSCPGDE"
     gene            complement(1822..3447)
                     /locus_tag="PLY81_07620"
     CDS             complement(1822..3447)
                     /locus_tag="PLY81_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF30130.1"
                     /translation="MKWLIKIKLIWLFFYISFLNYGCTQNNNSSKKIFSYNETAGIAT
                     LDPAFAKNQSIMWAVHQLYNTLVEVDSNLQIVPSLAKSWSISEDKRTYTFYLRNNIFF
                     HDNQAFKNGKGRKMNANDIVYSFQRIINKKTASSGAWIFNNKVDTIEPYKAINDTTFQ
                     LKLLQPFHPILGILSMQYCSIVPKEVVEKFGKDFRNNPCGTGPFKFFMWQEGQALVLH
                     KNESYWEKDLDGTPLPKIDAIKVSFFDNKATEFLQFRQGKLSFINDIDASFKDEVLTK
                     NGLLRKNWQNKIKLNKHAYLNTEYLGILMDENNELVKSSPLKIKAIRKAINYAINKEQ
                     LMLYMRNSIGFAANNGFIPYGLPANKLQDKFFEYNPQKAKKILDSIQYNYTTIPITLL
                     TIPVYADVASFITKQCELVGIKMQVEVIQKSLLLEQTAKSKAMFFRGSWIADYPDEEN
                     YFAVFYSKNPAPPNYTRFNNKTYDKLYEQALQETNDSARLNLYKALDNIIKEELPIIP
                     IWYDMIIHLVNNNVQNFTPNSLNLLELRRVVIE"
     gene            3512..>3694
                     /locus_tag="PLY81_07625"
     CDS             3512..>3694
                     /locus_tag="PLY81_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30131.1"
                     /translation="MTLLLRRTLVLTVMLLSSFSVLYVNNTSAQILNKKESFNADDSL
                     RGTLNANRNWWNVLRYD"
BASE COUNT         1284 a          518 c          532 g         1360 t
ORIGIN      
        1 aattttagat ttaaggctgc aaagttaagg aagttttcag ctcatcttct ttaaaagaga
       61 tttagttgaa attcatctgt tttttaaaat ataattaata gcttatcatt gtattttaaa
      121 atatgtacaa taaaaaaaac aatatattaa ttaagtacgc aggtatggca acacaactat
      181 tagttgggct tgggttaact acatggtttg gcaactggat agataaaaaa aatatattca
      241 aaaagcctgt ttttacctgg attttacctt taatatttgt aattggattt ttaataaaat
      301 taataaaaga cttaaataaa aaataatgtt tcagcaatct aaatttggca aattaataat
      361 gccatttttt gccttgttta ctttagtgaa tattgtattt attacttggg cagaaaaatg
      421 ggatgccatt aaacttaatc atactgttat tatttttggg aatgcattgc tgtttttact
      481 ttccatattt agtatttgga tgcatttaaa agctgtaaat cataaaaacc catcagtaat
      541 ggtaagagga gttttaggca gcacttttgt aaaaataatt ttaatagcca cttctatttt
      601 attgtatttg tattttactc ctaaagaaca tcaaagcaaa ttagcagtta ttgcagtaat
      661 ggttttatat ctcatttatg tatttattga agttagggta gttgtaaggc taaataaaaa
      721 gaaagttaag taatgccaat agttgagaaa cctttaaata gtttagctca ttttttacct
      781 gatgaaagtt tggatattgt tcttcaattt attcaacaat acaaaattca tttgcttgtt
      841 actcaaaaaa gaaaaaccat tttaggaaac tataaacatg caggcttaca aagcaatcat
      901 aaaatttcgg ttaatggtaa tttgaataag tatgagtttt taattacttt tttacatgaa
      961 ttagcccatt tgcttaccta cgaacaattt aaaaataaag tagagcctca tggaaaagag
     1021 tggaagtttt tttatagtaa actattaact gagtttgttc aactaaaaat tttcccaaac
     1081 gatatagtta tagctcttca aaaatctatt ttaaatcctg ctgctactgc taatggcgaa
     1141 acagatttac tacgtgtttt aagaaaatac gatactgcta ataaccctca attacaaatg
     1201 attgaaaata ttccaactca ttctaaattt agtattgaga atggaaggat ttttattaaa
     1261 gggcaaaaaa gaagaaaaag gatagaatgt atagaaataa gcaccaacaa aatttattta
     1321 tttaatcctt tggtagaagt aaaactgttt gagtaattat agcacagtta tttgtatttt
     1381 gcacttactt taaacttttt gttatgaaga aagctaagcc atttattgcc cttgttattt
     1441 tagtggtagt cgctattttt ttaagaaact gtgtaagcaa taaatctaac aagaaagagg
     1501 tagatgaaaa tgtaaaacaa agaggtttaa accgaaatcc atctaatata aattacacta
     1561 aacatgcaaa atgcagaatg gattgtaggc atattgatga aaaagaagta gtgtatattt
     1621 tagaaaatgg aaaaattaat tacagcaaaa gtgatttaaa aggtaaagat tgcaataaaa
     1681 aatatgctgt agaaggtatt agtaaagaca aacaaaaatt gagaatagtt tttgccccat
     1741 gcaacaacga agttactgtt ataacctgta ttgatttaga tacagaatgg gagtgtagct
     1801 gccctggtga tgagtaaata attattctat aacaaccctt ctcaattcta gcaagtttaa
     1861 agaattaggg gtgaaatttt gtacattatt gtttaccaaa tgtataatca tatcgtacca
     1921 tattggtata attggtaatt cttcttttat aatattatcc aatgccttgt ataaatttaa
     1981 tcttgcacta tcattagttt cttgcaatgc ttgttcgtat aatttatcat aagttttatt
     2041 gttaaatcta gtatagttgg gtggtgcagg gttcttacta taaaatactg caaaataatt
     2101 ttcttcatca gggtaatcgg caatccaact acctctaaaa aacatggctt tacttttagc
     2161 agtttgttct aacaataaac ttttttgaat cacttccact tgcattttta tcccaaccaa
     2221 ttcacattgc tttgtaataa aacttgcaac atctgcatat acaggtatag ttaataacgt
     2281 aataggtata gttgtataat tgtactgaat gctatctaat attttttttg ctttttgagg
     2341 gttatattca aaaaacttat cctgtaattt gttagcaggt aaaccataag gtataaaacc
     2401 attattggca gcaaagccaa tgctgttacg catgtacagc attaattgct ctttgttaat
     2461 ggcataatta atagcttttc tgattgcttt aatttttaat ggagagcttt ttaccagttc
     2521 attattttca tccattaata ttcctaaata ttcagtattc aaataggcat gtttgttaag
     2581 tttaattttg ttctgccaat tttttctcag tagcccgttt ttggttaaaa cctcatcttt
     2641 aaaactagca tctatatcat ttataaaact taatttacct tgcctaaatt gtaaaaattc
     2701 tgttgcctta ttatcaaaaa aacttacttt aattgcatct attttgggta atggtgtgcc
     2761 atccaaatct ttctcccaat agctctcatt tttgtgcaat accaatgctt gcccctcttg
     2821 ccacataaaa aatttaaatg gccctgtgcc acaagggtta tttctaaaat ctttaccaaa
     2881 tttctctacc acttcctttg gtacaatact acaatattgc atgcttaaaa tacccaaaat
     2941 aggatgaaaa ggctgtaata gttttagttg aaaagtagta tcgttaatag ctttatatgg
     3001 ttcaatagta tctactttat tattgaaaat ccatgcccca ctacttgcag tttttttatt
     3061 aataattctc tgaaaactat agacaatatc attggcattc atttttcttc ctttaccatt
     3121 tttaaatgct tggttgtcat gaaaaaaaat attgtttctt aaataaaaag tgtatgttct
     3181 cttatcctca ctaatgctcc aactttttgc taaggatggt acaatttgca agttgctatc
     3241 cacttcaacc aatgtattat acaactgatg tacagcccac ataatacttt gattttttgc
     3301 aaatgcaggg tctaaagttg caatgcctgc agtttcgtta taactaaata tttttttact
     3361 actattgtta ttttgtgtac aaccatagtt taaaaagctg atataaaaaa acaaccaaat
     3421 taattttatt ttaataagcc atttcattac aacgaatatc ttacttttaa tgttttaaag
     3481 atatttttat ttaacaaact ttatacttgc tatgacactt ttgttgcgtc gcacccttgt
     3541 gcttactgta atgttactga gcagttttag tgtactatat gtaaataata cttcggcaca
     3601 aattttaaac aaaaaagaaa gttttaatgc tgacgatagt ttaagaggca cattaaatgc
     3661 aaacagaaat tggtggaatg tattgcgata tgat
//
LOCUS       DAOQHP010000227         3648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_15930_length_3648_cov_4.097133, whole genome shotgun sequence.
ACCESSION   DAOQHP010000227 DAOQHP010000000
VERSION     DAOQHP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15930_length_3648_cov_4.097133"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..924)
                     /locus_tag="PLY81_07630"
     CDS             complement(4..924)
                     /locus_tag="PLY81_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNF30132.1"
                     /translation="MIILGINAYHADSSVAIFKDGIMIAATEEERFRRIKHWAGFPSM
                     AIEFCLKEVGVTLKEVDHIAIGRDPKAKFLKKLFFFAQHPVHSIKFVAERWSNQKQVT
                     SIEKELAQLSGLDETEIKNKIHQVEHHRSHLASAFFASPFEEAALLSIDGSGDFSTTM
                     LGIGKGNKIEVIDSVDFPHSMGIFYTAFTQLLGFPYYGDEYKVMGLAPYGEPKYVDKL
                     RDVVLWKDNGLFQLNFAYFRSATQGFIKYENNLPIVPPLFGKKMVEVFGAERKKDEPL
                     SQYHKDVAASVQRFTEETIFHRGLLHIRGI"
     gene            complement(1065..2087)
                     /locus_tag="PLY81_07635"
     CDS             complement(1065..2087)
                     /locus_tag="PLY81_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF30133.1"
                     /translation="MPKKKILILYEYFYPGFKAGGPIQSLVNMVVALQHQYEFYVATT
                     AYDLQTATPYQNVVCNKWVEVQLTPNALPVKVWYSSNIKPGYKELLQTILTSGCTTIY
                     INGFYTNYFLFPLLYLKLGKLKNMDCIVAPRGMLTKGALSVKPFRKKIYFTVIKCFGL
                     LKTVRYHATNTEEVQEIQSMFGRSSSITLASNVSKPPFEKFRQSTKNNDELRLVFLAL
                     ISPMKNLLLLLQYLQSCSNTIVLDIYGPVKDKAYWQQCLQAIQALPKNIKVHYKGDAE
                     PHTVQNIFTGYDAMILITKGENFGHAIYESLSVGTPVIISHFTPWNNLQEKKAGWNID
                     INNKEE"
     gene            complement(2080..3174)
                     /locus_tag="PLY81_07640"
     CDS             complement(2080..3174)
                     /locus_tag="PLY81_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF30134.1"
                     /translation="MLFISLDTFLYTGGLEKFNRCFLKVLQELSNESFIKAKAMALYD
                     KNCDEKYFNPAHYTAFKGNKLKSVLYTLLHSYKHKTVILGHINLALIGLLIKLFNPTT
                     KVILIVHGIDIWQPQTGIKKRFLHTVNEIWSVSNYTKQTMLQNNPYIQEQKIKIFPNT
                     LDPYFQLPQNFEKPDYLLQRYGISKETKIIVTVTRLAATEKFKGYDGIISLLKSLNKQ
                     EKIQYVLCGKTDEKELERIKHLIKEQEAEDFITLTGFVKDEELIDHYLLADVFAMPSK
                     KEGFGIVFIEAAACGCKVIAGNKDGSVDALQNGALGTLINPDSVEELEQAILENLNSN
                     HHHPAALQQKVVTSFGFPVFKERLKQYLNA"
     gene            complement(3204..>3648)
                     /locus_tag="PLY81_07645"
     CDS             complement(3204..>3648)
                     /locus_tag="PLY81_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30135.1"
                     /translation="FNIPDNTAFLNGATIRDAILTSLYLSSFAENKVVGGGKATYLKL
                     SGFELTSSTSMGVGIIGESYGNFGVIGSYWFMLIWGFILGLYLYMLFKKSNDYYTLPL
                     WIPVLFLQVVKAETDFYTVFNHLFKSTILVFAVLYFIRKKYRLAV"
BASE COUNT         1185 a          611 c          517 g         1335 t
ORIGIN      
        1 taattatata ccgcgaatat gtaaaaggcc gcgatgaaat atggtttcct cagtgaatcg
       61 ttgtacagag gctgctacat ctttatggta ttgtgataat ggttcatctt tttttctttc
      121 tgctccaaaa acttcaacca tttttttacc aaataatggt ggtacaatag gcaaattgtt
      181 ttcatatttt ataaagccct gtgttgctga acggaaataa gcaaagttta attgaaataa
      241 tccattgtct ttccataaaa caacatctct taatttatca acatacttag gttcgccata
      301 aggtgctaaa cccattactt tatactcatc tccataataa gggaagccta aaagttgtgt
      361 aaaagctgta taaaaaattc ccatagaatg agggaaatca acagaatcaa ttacttcaat
      421 tttattgcct tttccaatgc ctaacatggt tgttgaaaaa tcgcctgagc catcaatact
      481 aagcaaagca gcttcttcaa aaggtgaggc aaaaaaggca cttgctaaat ggcttctatg
      541 atgctctacc tgatgaattt tattttttat ttcagtttca tctaatccag aaagttgtgc
      601 taattctttt tcaatacttg ttacttgttt ttgattactc catctttctg caacaaattt
      661 tatggagtga acagggtgct gtgcaaagaa aaaaagtttc tttaaaaatt ttgctttagg
      721 gtctcttcct attgcaatat gatctacttc tttcagagta actcctactt cctttaaaca
      781 aaactctatt gccatagatg gaaaaccagc ccagtgtttt attcttctaa aacgttcttc
      841 ttctgttgca gcaatcataa ttccatcttt aaaaatagca acagatgagt ctgcatgata
      901 tgcattaata cctaaaataa tcatagtttt taactaaata aattgttgta attttttgta
      961 tattttgatt gttcataata attttttgca acctgccaag cattgttgca ataaacatta
     1021 tgttcctgta atgtcatttt tgataactga tgtaaggtgg ttagttattc ttctttatta
     1081 tttatatcaa tattccaacc tgcttttttt tcttgcaaat tattccatgg tgtaaaatgg
     1141 cttataatta caggtgtacc tacacttaaa ctttcataaa tagcatgacc aaaattttcg
     1201 cctttggtta ttagaatcat tgcatcgtag cctgtgaaaa tattttgcac tgtatgtggc
     1261 tctgcatcgc ctttataatg tactttaata ttttttggta aagcttgtat agcttgtaaa
     1321 cattgttgcc aataggcttt atcttttaca ggaccataaa tatccaacac aatagtatta
     1381 ctgcaagact gtaaatattg taatagcagt aataaatttt tcatcggtga tattagagct
     1441 aaaaaaacta atcttaactc atcattattt ttggtagatt gcctgaattt ttcaaaagga
     1501 ggtttagaaa catttgaagc taaagtaatt gaagaacttc ttccaaacat agattgtatt
     1561 tcttgtactt cttctgtatt ggtagcatga taacgaacag tttttaaaag accaaaacat
     1621 ttaattacag taaaataaat tttctttcga aatggtttta cactcaatgc cccttttgta
     1681 agcatacctc ttggagcaac aatgcaatcc atattcttta attttcccaa ctttaaatac
     1741 aataatggaa ataaaaaata attggtataa aaaccattga tataaatagt agtacagcca
     1801 gatgttagta tagtttgcaa taactctttg taccctggtt ttatattaga tgaataccaa
     1861 acctttacag gtaaagcatt tggtgtgagt tgcacctcaa cccatttatt gcacactacg
     1921 ttttgatagg gtgttgcagt ttgcaaatca taagcagtgg tagcaacata aaattcatac
     1981 tgatgttgta gagctacaac catatttacc aaagattgta ttggaccacc agctttaaac
     2041 ccaggataaa aatattcata taaaatcaat atttttttct taggcattca ggtattgttt
     2101 tagtctttct ttaaaaacag gaaatccaaa actggttacc actttttgct gtaatgctgc
     2161 aggatgatga tggttactat ttaagttttc aagtattgct tgttctaatt cctctacact
     2221 atcaggattg attaatgtac ctaatgcacc attctgtaaa gcatctacac tgccatcttt
     2281 attgcctgct attactttac aaccacaagc agcagcttct ataaacacaa ttccaaagcc
     2341 ttctttttta ctgggcatag caaaaacatc tgccaataag taatggtcta tcagctcttc
     2401 atcttttaca aagccagtaa gtgttataaa gtcttctgcc tcttgctcct taattaaatg
     2461 ttttattctt tctaactctt tttcatcagt tttaccacat aaaacatact gtattttttc
     2521 ttgtttattt aagcttttta aaagtgaaat tattccatcg tatcctttaa atttttctgt
     2581 tgctgcaagc cttgttacag taactattat ttttgtttcc ttagaaatgc catatctctg
     2641 caaaagataa tcaggctttt caaaattttg tggtagctga aaatagggat ctagagtatt
     2701 gggaaatatt ttaatttttt gttcctgtat ataagggttg ttttgcaaca tagtttgttt
     2761 tgtataattg cttacactcc aaatttcatt tacggtatgt aaaaaccttt tctttattcc
     2821 tgtttgaggt tgccatatat ctataccatg cactattaat attactttgg ttgtaggatt
     2881 gaaaagtttt ataagcagcc ctattaatgc aagatttata tgacccagaa ttactgtttt
     2941 gtgcttgtat gaatgtaaca gagtatataa tacagatttt aatttattac ctttaaaagc
     3001 tgtataatgg gcagggttaa aatatttttc gtcacaattt ttatcgtaca gagccattgc
     3061 ttttgcttta ataaaacttt cgttggaaag ttcttgcaaa acctttaaaa agcaacgatt
     3121 aaatttttct aacccaccgg tatataaaaa agtatctaac gaaataaaaa gcaatgtgta
     3181 attagagatt taatttaatt gaattagact gccagtctgt atttttttct gataaaatat
     3241 agtactgcaa aaacaagtat ggttgattta aataaatgat taaatactgt ataaaaatct
     3301 gtttctgcct taactacttg taggaataaa acaggtatcc ataatggtaa agtataataa
     3361 tcgttacttt ttttaaatag catgtataaa tacagcccta atatgaaacc ccaaataagc
     3421 ataaaccaat agctaccaat aaccccaaaa tttccataag actctcctat tatgccaaca
     3481 cccatagatg tactacttgt aagttcaaag ccactcaatt ttaaataagt tgccttacct
     3541 ccaccaacta ctttgttttc tgcaaaagaa gatagatata aacttgttaa tatagcatct
     3601 cttattgtag ccccatttaa aaaggctgta ttatctggta tattaaac
//
LOCUS       DAOQHP010000228         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16156_length_3616_cov_3.705981, whole genome shotgun sequence.
ACCESSION   DAOQHP010000228 DAOQHP010000000
VERSION     DAOQHP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16156_length_3616_cov_3.705981"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..730
                     /locus_tag="PLY81_07650"
     CDS             14..730
                     /locus_tag="PLY81_07650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNF30136.1"
                     /translation="MKLFKKKNKEENLITPEFKEFEEIYNLCKPYTMTGIERMFALYK
                     ATEYIVKNNIEGDFVECGVWRGGSSMVILKTLQKFNVTNRKIWLFDTYEGMSEPTEDD
                     KDFTDLSADKQLQKSKKDADSFIWAYATLEDVKKNIELTKYPLENVHFIKGKVEDTIP
                     EKNYFNHCSLLRLDTDWYESTKIELEYFYPLLQKNGVMIIDDYGHWQGCKKAVDEYFA
                     TQNFCPLLQRIDYTGRIMTK"
     gene            736..1452
                     /locus_tag="PLY81_07655"
     CDS             736..1452
                     /locus_tag="PLY81_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30137.1"
                     /translation="MQTKVTKQFNPPLTSAYYFIRKLLLHKIIEYKNHLQGNLLDFGC
                     GSKPYKSLFNHVSSYTGVDYENTGHSHINEQIDVFYDGKNIPFKDNEFDSILCSEVFE
                     HVFNLEEILPDLNRVLKPGGKILITCPFVWNEHEVPYDYARYTQFALKHLLEKNGFKI
                     IVLDKSGDFATTLHQMKMVYISEHFLQSIFIIGKLKFITSNIRSPLMFLCNGLFLLLH
                     KLMPKRNDLYLNNIVIAEKK"
     gene            1478..2251
                     /locus_tag="PLY81_07660"
     CDS             1478..2251
                     /locus_tag="PLY81_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF30138.1"
                     /translation="MQKISAAIITFNEEKNISRCIESLLQIADEIIVVDSLSTDNTKN
                     ICSKYPIKFIEQPFLGYIEQKNFAVALTYFDYVLSLDADEALDETLQQSILAIKQKKV
                     VADSYKMNRCTNFCGKWIKHGTWYPDKKIRLFNKHNAKWAGTNPHDFVEIDKQSSCIY
                     IKGDILHYSYNSLEEVITQANKFTTIQANAMYKKGKRASVINLIVNPCVAFISGYFIK
                     LGFLDGKDGFILAKTIAYQTLLKYAKLLHLQKANKHNNI"
     gene            complement(2326..>3616)
                     /locus_tag="PLY81_07665"
     CDS             complement(2326..>3616)
                     /locus_tag="PLY81_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF30139.1"
                     /translation="KSPYGGSFTWQNNATDTFQVVKNTGTYWTKYTTPNGCYVYDTVE
                     ITKVQMPYPQFGNDTAFCANSSVTLTATNYTGATYLWNTGATTNTINVNSSGTYSIIS
                     TIGLCESKDTINVIKSDLPIKNLGNDTTYCGNNGIVLNATYPGATTYLWNTGATTATI
                     NITSTGTYSVTNNLNGCIAKDTIKITINPIPYPQMLISDTALCPYENINLNAFYPGAT
                     YLWNTGETTNIINAKTGGLYIVTNTLNGCIAKDTVNIKNNKAAIAYAGNDVLMYAGSS
                     VNLTASQNANNKTYQWTPLNNLSSPNYYTTNASPKDTTDVFYYLKVTSVDNCVAYDTI
                     LVKIMNAKFDIPNAFSPNGDGINDLWEVPFLYSFPTSRMQIFNRNGQIIYTSGGTNYK
                     PWNGMYNGKPAPIGVYYYVIEVGNGFPRKTGWVAIVR"
BASE COUNT         1307 a          506 c          563 g         1240 t
ORIGIN      
        1 caaaaaataa tttatgaaac ttttcaagaa aaaaaataaa gaagaaaatt taattacacc
       61 agagtttaaa gagtttgaag aaatatataa tttatgtaag ccttatacca tgactggtat
      121 agaaagaatg tttgctctat acaaagccac tgagtatatt gttaaaaata atattgaagg
      181 cgactttgta gaatgtggtg tttggcgtgg aggaagctct atggtaattt taaaaacatt
      241 acaaaaattt aatgtaacca atagaaaaat atggctattt gatacctatg aaggcatgag
      301 tgaacctaca gaagatgata aagattttac cgatttaagt gctgataaac aattacaaaa
      361 atcaaagaaa gatgcagata gttttatttg ggcttatgct actttagaag atgtaaaaaa
      421 gaatattgaa ctaactaaat acccattaga aaatgttcat tttattaaag gaaaagtaga
      481 agacactatt cctgaaaaaa actattttaa ccactgttct cttttaaggt tagatacaga
      541 ctggtacgaa tctactaaaa tagaattaga atatttttac ccactattac aaaaaaatgg
      601 agtaatgatt attgatgatt atgggcactg gcaaggttgc aaaaaagcag tggatgaata
      661 ttttgcaacc caaaattttt gtcctttact acaaagaatt gattacaccg gcagaataat
      721 gaccaaataa attatatgca aactaaagtt accaagcagt ttaatccacc tttaactagt
      781 gcgtattatt ttataagaaa gcttttgctg cataaaatta ttgaatataa aaatcactta
      841 caaggtaatt tgcttgattt tggttgtggc tcaaaaccat ataaaagttt atttaaccat
      901 gttagctctt atacaggagt tgactatgaa aacacaggcc attctcacat caacgaacaa
      961 atagatgtat tttatgatgg caaaaatatt ccttttaaag ataatgaatt tgactctata
     1021 ctgtgtagcg aggtttttga acatgttttt aatttagaag aaattttacc tgatttgaat
     1081 agagttttaa agcctggtgg taaaatttta attacctgtc cttttgtttg gaacgaacat
     1141 gaagttcctt atgattatgc cagatatacg cagtttgcat taaagcattt attagaaaaa
     1201 aatggtttta aaataattgt tttagacaaa tctggagatt ttgccactac actacaccaa
     1261 atgaaaatgg tttatataag tgagcatttt ctgcaatcta tttttatcat tggcaaacta
     1321 aagtttataa catccaatat caggtcacct ttaatgtttt tgtgtaatgg gctattttta
     1381 ttattgcaca aactaatgcc caaacgcaat gatttatatt taaataatat tgtaattgca
     1441 gaaaaaaaat aatacataac ttttccaaaa tattctcatg caaaaaattt cggctgcaat
     1501 aataactttt aacgaagaaa aaaatataag ccgatgtatt gaaagcttat tacaaattgc
     1561 tgatgagatt attgtggttg actccctttc aacagataat acaaaaaata tttgcagtaa
     1621 ataccctatt aaatttattg agcagccttt tttaggttat atagagcaaa aaaattttgc
     1681 agtagcacta acttattttg actacgtttt aagtttagat gccgatgaag cattagacga
     1741 aactcttcaa caatctattt tagcaattaa acaaaaaaag gttgttgcag atagttataa
     1801 aatgaatagg tgtaccaatt tttgtggcaa atggataaaa catggcacat ggtatcctga
     1861 taaaaaaatt aggttattta ataagcataa tgcaaaatgg gctggcacaa acccacacga
     1921 ttttgttgaa atagataagc aatcaagttg tatttatatt aaaggagata ttttacatta
     1981 tagttacaat agtttagaag aagttattac acaagcaaat aagtttacaa ctatacaggc
     2041 taatgcaatg tataaaaaag gtaaaagagc ttctgtaatt aatttaattg taaacccatg
     2101 tgttgcattt ataagtggtt attttattaa gcttggtttt ttagatggta aagatggatt
     2161 tatattagct aaaaccattg cctatcaaac cctattaaaa tatgctaaac ttttacactt
     2221 gcaaaaagca aacaagcata ataatattta aaaaaatgta attaaataga tgactatttt
     2281 gaaccctaat tccaactaag aatttaaaat tttcttggta attaattatc taacaattgc
     2341 tacccaaccc gtttttctag gaaaaccatt gcctacctct attacataat aataaactcc
     2401 aattggtgct ggtttgccat tatacattcc attccatggc ttgtaattag tacccccact
     2461 tgtataaatt atttgtccat ttctattaaa aatttgcatt ctagaagttg gaaaagaata
     2521 taagaaagga acttcccata ggtcatttat gccatcgcca tttggtgaga atgcatttgg
     2581 aatatcaaac ttagcattca ttattttaac taaaatagta tcatacgcta cacaattatc
     2641 tacagaagta acttttaaat aataaaacac atctgtagta tctttaggac ttgcattggt
     2701 ggtataataa ttagggcttg ataaattatt taatggagtc cattgatagg ttttattatt
     2761 tgcattttga gaagcagtta gatttacaga gcttccggca tacattaata catcgttacc
     2821 tgcataagca atagcagctt tattgttttt aatatttact gtatcttttg caatgcaacc
     2881 atttaaagtg ttagtaacaa tataaagtcc gccagttttt gcattaataa tattggttgt
     2941 ttctcctgta ttccataaat aagtagcccc tgggtaaaat gcatttaagt ttatattttc
     3001 ataggggcat aaagctgtat cacttattag catttgtgga tatggtatag gattaatagt
     3061 aatttttatt gtgtcttttg caatacaacc attcaaatta tttgttacag aataagtacc
     3121 agtactggta atattaatgg tagctgttgt tgcacctgta ttccataaat aggttgtagc
     3181 acctgggtag gttgcattta aaacaatacc attatttcca caataagtag tatcatttcc
     3241 aagatttttt attggtaaat cagatttgat aacattaatc gtatcttttg attcgcaaag
     3301 cccaattgta cttataatag aataggtgcc tgaactattt acattaattg tgttggtggt
     3361 tgctcctgtg ttccataaat aagtagcccc ggtataatta gttgcagtta aagtaacact
     3421 agagttggca caaaaagcag tatcatttcc aaactgtgga taaggcattt gcacttttgt
     3481 tatttcaaca gtatcataaa cataacaacc attaggggtt gtatattttg tccaatatgt
     3541 tcctgtgttt tttacaacct gaaaagtatc tgttgcattg ttctgccaag taaagctacc
     3601 tccgtaaggg ctttta
//
LOCUS       DAOQHP010000229         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16209_length_3609_cov_4.862971, whole genome shotgun sequence.
ACCESSION   DAOQHP010000229 DAOQHP010000000
VERSION     DAOQHP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16209_length_3609_cov_4.862971"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..556)
                     /locus_tag="PLY81_07670"
     CDS             complement(68..556)
                     /locus_tag="PLY81_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30140.1"
                     /translation="MVNSLFNWLLVGCIALLHPFYVSKVEINHNAKDKNVEISIRIFT
                     DDLELTLRKFGNTPINLNAPKDIVATDKLINKYIQDKLRITIDDKISSIQYLGFEINK
                     ESVWIYYEIPQINTIKKVGINCSLLYDYKQEQMNIIQLTANGKDVSYKLDNPKSYVSF
                     SF"
     gene            668..1249
                     /locus_tag="PLY81_07675"
     CDS             668..1249
                     /locus_tag="PLY81_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNF30141.1"
                     /translation="MGNFQLYFDIGFKHIVNIGALDHILFMAAIALRYQFFDFKKLLI
                     IVTAFTIGHAITLALAILQLISLPKNWIEFLIAVTIIVTSFSNLFVKKFVYKGKFPPI
                     YYFALIFGLIHGMGFASEFVALEGTGLSAALNLLYANIGIEVGQLLFVIVVLVISFIC
                     LNILKLNRREYILFTSGAIFGVALEMALTRLPF"
     gene            1260..>3609
                     /locus_tag="PLY81_07680"
     CDS             1260..>3609
                     /locus_tag="PLY81_07680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF30142.1"
                     /translation="MKKVIVGIIMLLGTNAFSQNIQNNPNSNHGNKFEQLGIILPTPN
                     SYRAASGAPGGKYWQQRCDYNIVCELDEKKLTLTGFETLTYFNNSPDVLNYLWLQLDE
                     NQHSTINNANYQESNMLNTTLSVAELQRMEDAKKDNGYGTQITKVTDATGNLLSYTIN
                     KTMMRVDLKTPLKPGQQFVFKINWFYKMANRMSMGGRGGYEFFPEDGNHIFTMTQWFP
                     RLCVYSDFQGWQNHQFTGRGEFALTFGNYKVQITVPADHIVMSTGECNNYNQMLTATQ
                     QQRWQKAQTTNEPLEIVTLNEATKAEATKSSKKKTWIYTANNVRDFAWCSSRKFIWDA
                     MPVKIEGKKVMCMSAYPKEAYHLYRKFSTKAVAHTIKTYSDFTIPYPYPVAQSIEASN
                     GMEYPMICFNFGRTEKDGTYSEATKNGMIGVIIHEVGHNFFPMIINSDERQWSWMDEG
                     LNTFVEYLTEELWDNKFPSKRGPAFSITDYMRLPKDQLEPIMTNSENIIQFGPNAYSK
                     PATGLNILRETIMGRELFDFAFKQYTKRWAFKHPEPADFFRTMEDASGEDLDWFWRGW
                     FFSTDACDIALDSVKYATYDATPPTERTMKNKPRALDKPLVPLFDDISKIRNREDKKI
                     VFATDADTSLRDFYWRYARGIEPYDTTKYLPEIGNAAIMQPMDIATQEKYTNTHLYEL
                     SFINKGGLVMPIIVEFTFEDGTKQLEKIPAQIWRKNEHKVTKVFMTPQKAVSITLDPM
                     KETADIDESNNTWFAKNETVQPSKFKIFKSGPFNRRNMMGPQK"
BASE COUNT         1324 a          534 c          586 g         1165 t
ORIGIN      
        1 aatggaaatt ttaccacaaa atttttagaa acatttaaac taaaataatt ttataagaaa
       61 tattaaacta aaaactaaaa ctaacatagc ttttaggatt atctaactta tagcttacat
      121 ctttaccatt tgctgttagc tgaataatat tcatttgttc ttgcttataa tcatataaaa
      181 gagaacagtt aatgccaact ttttttatgg tatttatttg aggtatttca taataaatcc
      241 atacactttc cttatttatt tcaaagccta aatattgtat agaagatatt ttatcatcta
      301 tggtaattct taatttatct tgaatatatt tattgataag tttatcagtt gcaacgatat
      361 cttttggggc attaaggtta ataggagtat tgccaaattt tcgtaaagta agttccaaat
      421 catctgtaaa tattcttata gatatttcta catttttatc ttttgcatta tgattaattt
      481 ctactttact tacataaaaa ggatgtaata aagcaataca accaaccaat aaccaattaa
      541 ataaactatt taccatttaa aatcattatt ttgctaaggt aataaaccta cttaaatttc
      601 aagtttagaa aagaacatag ttggcttaat tttttaattt taatcaaata attactgact
      661 taaatctatg ggaaattttc agttatattt tgatattgga tttaaacata ttgtaaatat
      721 tggggcacta gaccatattt tgtttatggc tgccattgca cttagatacc aatttttcga
      781 ttttaaaaaa ctgctaatta ttgtaacagc ttttacaata ggccatgcaa taaccttagc
      841 attagccatt cttcaattaa ttagtttacc taaaaactgg attgagtttt taatagccgt
      901 tacaataatt gtaacatcat tcagtaattt atttgtaaaa aaatttgttt acaaaggcaa
      961 gtttccgccc atctattatt ttgcattaat ttttggttta attcatggta tgggctttgc
     1021 atcagaattt gttgctttag aaggcacagg cttatcggca gcactaaatt tattatatgc
     1081 taatattggt atagaagtag ggcagctttt gtttgtaata gttgttttag taatttcatt
     1141 tatttgtcta aatattctaa agcttaaccg aagagaatat attttattta caagtggtgc
     1201 tatttttggt gtagctttag aaatggcact aactcgttta cctttttaaa ttttttaaga
     1261 tgaagaaagt tatagtagga attataatgc ttttaggcac taacgccttt tcgcaaaata
     1321 ttcaaaataa tcccaatagt aatcatggta ataagtttga acaattaggc attatactgc
     1381 ctacgcctaa ttcctataga gcagcaagtg gtgccccagg aggtaaatat tggcagcaaa
     1441 ggtgcgatta taatattgtt tgtgaattag atgagaagaa gctaacactt accggctttg
     1501 aaacattaac ttattttaat aactcgccag atgtattaaa ttatttatgg ttacagttag
     1561 atgaaaacca acacagcact ataaataatg ctaattatca agagtctaat atgcttaaca
     1621 ctacactaag tgtagcggaa ttacaaagaa tggaagatgc taagaaagat aatggctatg
     1681 gtacacaaat tacaaaagta accgacgcta caggcaactt gttaagctat actattaata
     1741 aaaccatgat gagggttgat ttaaaaacac ctttaaaacc tggtcagcaa tttgttttta
     1801 aaataaactg gttttacaaa atggctaata gaatgtctat gggcggtcgt ggtggatatg
     1861 aattttttcc agaagatggc aaccatattt ttacaatgac acaatggttt cctaggcttt
     1921 gtgtgtatag cgattttcaa ggttggcaaa atcatcagtt tacaggcaga ggagagtttg
     1981 cattaacatt cggaaattat aaagtacaaa taacagtacc tgcagatcat attgtaatgt
     2041 caacaggaga gtgtaataat tataaccaaa tgctaactgc cacacaacaa caacgttggc
     2101 aaaaagcaca aaccacaaat gagccactag aaatagtaac cttaaacgaa gccacaaaag
     2161 ccgaagctac taaaagcagt aaaaagaaaa cttggattta tacagcaaat aatgttagag
     2221 attttgcatg gtgtagctct cgtaaattta tttgggatgc tatgcctgtg aaaattgaag
     2281 gtaaaaaagt aatgtgtatg agtgcctatc caaaagaagc gtatcacctt tatcgtaaat
     2341 tttctaccaa agcagtagct catactataa aaacatactc agattttaca ataccatatc
     2401 cttatcctgt agcacaaagt atagaagcaa gcaatggaat ggagtaccca atgatttgct
     2461 tcaattttgg aagaacagaa aaagatggaa cctattctga agctactaaa aacggaatga
     2521 taggagtaat tattcatgaa gtaggccata acttttttcc aatgattatt aatagcgatg
     2581 aacgccaatg gagctggatg gatgaaggct tgaatacttt tgttgaatac ttaaccgaag
     2641 agttatggga taataaattc ccctcaaaaa gaggccctgc attttctata acagattaca
     2701 tgcgtttacc caaagaccaa ttagagccta ttatgaccaa cagcgaaaat attatacaat
     2761 ttggtcctaa tgcttatagc aagcctgcta cagggttaaa tattttaaga gaaacaatta
     2821 tgggcagaga gttatttgat tttgcattta aacaatatac caaaaggtgg gcatttaaac
     2881 atccagaacc tgccgacttt tttagaacaa tggaagatgc aagtggagaa gatttagatt
     2941 ggttttggcg aggatggttt tttagtacag atgcttgcga tattgcactt gatagtgtaa
     3001 aatatgctac ttatgatgca accccaccta cagaaagaac aatgaaaaac aaaccaagag
     3061 cattagataa gccacttgtt cctttatttg atgatatttc taaaattaga aacagagaag
     3121 ataaaaaaat tgtttttgca accgatgcag acacttcttt aagagatttt tattggcgat
     3181 atgcaagagg cattgaacca tacgatacaa caaaatattt accagaaatt ggtaatgctg
     3241 ccattatgca accaatggat attgctaccc aagaaaaata tacaaataca catttatacg
     3301 aattaagctt tattaataaa ggcggcttgg taatgcctat aatagtagaa tttacttttg
     3361 aagatggcac caaacaatta gaaaaaatac ctgcacaaat ttggcgtaaa aatgaacaca
     3421 aggttactaa agtatttatg acaccccaaa aagcagtttc aataacatta gaccctatga
     3481 aagaaactgc agatattgat gaatctaata atacttggtt tgctaaaaat gaaacagtac
     3541 aacctagcaa atttaaaatt tttaaaagtg ggccatttaa cagaagaaat atgatggggc
     3601 ctcaaaaaa
//
LOCUS       DAOQHP010000230         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16217_length_3608_cov_5.032086, whole genome shotgun sequence.
ACCESSION   DAOQHP010000230 DAOQHP010000000
VERSION     DAOQHP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16217_length_3608_cov_5.032086"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1419
                     /locus_tag="PLY81_07685"
     CDS             376..1419
                     /locus_tag="PLY81_07685"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF30143.1"
                     /translation="MQEVKDIEQPIDELYERRTFIIDKGQEPLRIDKWLQIRTEGSTR
                     NKIQQAIDNGFVTVNNQKIKSNYKVKPLDEILVLSYLNPEYSELKIENIPLNIVYEDE
                     DVLVINKPSNMVVHPGVGNHTGTLLNGVAYHLLQQNPSLNEEELPRFGLVHRIDKNTT
                     GLIVLAKNGMAAAHLAKQFFNHTVKRNYVALVWGNLEANEGTINTHIARHKQYRKMFD
                     AYPDGEIGKNAITHYKVIERMNYVTLVSCQLETGRTHQIRVHMKFIGHTLFNDWEYGG
                     DKILKGTIFTKYKQFVDNCFAICPRCALHAKTLGFIHPTTQKEMFFEAELPEDMKNVI
                     SKWRGYVGNKQLD"
     gene            complement(1458..2309)
                     /gene="panC"
                     /locus_tag="PLY81_07690"
     CDS             complement(1458..2309)
                     /gene="panC"
                     /locus_tag="PLY81_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNF30144.1"
                     /translation="MVICKTAAILENTLKKYYTNNINIGFVPTMGALHNGHIALIEES
                     KQQCAITVASIFVNPTQFNDKNDFEKYPITIENDIFLLEKAGCDIIFLPSVNEIYSDG
                     IDNLKHYELSYLEEILEGKYRKGHFQGVCNVVERLLLMVKPTILFLGQKDYQQCMVVS
                     KLVELMNWQNKLKLVIVPTIREENGLAMSSRNRRLTEEERTKAAAIYNNLLYMKNNFE
                     RLPFNGLIKRAEEELLKNGFLKIDYISICNAQNLQPIAEYKEGVKLVVLIAAFIGEVR
                     LIDNLLL"
     gene            2483..>3608
                     /locus_tag="PLY81_07695"
     CDS             2483..>3608
                     /locus_tag="PLY81_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNF30145.1"
                     /translation="MSVQNNILSSPIEYLKGVGPLRADWLKKELNIFTFNDLLHLFPY
                     RHIDKSTITAINNIETNAEYIQVVGKLTNLQIIGERKGKRLTATLKDETGSIELVWFQ
                     GISWIQKILTEGSIYLVFGKLSYFNNTPQIIHAEIELYVPLQNNERKYFEPVYPSTEK
                     LKAKGLNGRQIAKLTFALWQQITIESIPENIPENILTELNLLNRYKAYKSIHYPANKI
                     EYNQALNRLKFEEFFIAQVRLNLVKIQRHQCSKGVIFEKVGALFNNFYSNHLPFELTN
                     AQKNVLKQIRQDAAKGHQMNRLLQGDVGSGKTIVALLSMLLAADNGYQSCLMAPTEIL
                     AMQHYNSLNSLLQPMNISVSLLTGSTKAKERKAILTDLQEG"
BASE COUNT         1375 a          539 c          538 g         1156 t
ORIGIN      
        1 atctggtaag gaataatcta attaaactac atcaggtttt tcgtgcaggg cagcaataaa
       61 agttttagat gactcaaatt ttttaatctg ataatcaggg tttaagctca aagtgtacga
      121 taacattgaa ctgtaccata aatcatcttc tacgatgaat attttgaagg aacgtggttc
      181 tttcataaaa tgagatattg gatatacttc tataaacgca aaacttttct caaaattgta
      241 taaatggaaa ttttatggga aatattttcc caaataaaga attttccatt tttgaaatag
      301 atacaagaaa tgttacctta attaaaattt ttaactttaa tagtgtacaa aaagtactgc
      361 ttacatttgc aaaatatgca agaagtaaaa gatattgaac aaccaataga tgagttatac
      421 gaacgtagaa cttttattat tgacaaaggg caagaacctc ttagaataga taaatggctg
      481 caaataagaa cagaaggaag taccagaaac aaaattcagc aagcaataga taatgggttt
      541 gtaactgtaa acaaccaaaa aataaaaagt aattataaag taaaaccttt ggatgaaata
      601 ttggttttaa gctaccttaa tccagaatac tcggagctta aaattgaaaa tattccacta
      661 aatattgtgt atgaagatga ggatgtactg gtgattaata aaccttccaa tatggttgtg
      721 catcctggcg ttggcaacca tacaggtaca ttattaaatg gtgttgctta ccatttgtta
      781 caacaaaacc cttctttaaa tgaagaagaa ctaccacgat ttggtttggt acatagaatt
      841 gataaaaata caaccggctt aattgttttg gctaaaaacg gaatggcagc agcacattta
      901 gctaaacagt tttttaacca tacggtaaaa agaaattatg ttgcattggt ttggggaaat
      961 ttggaagcaa atgaaggaac aattaatacg catattgcaa ggcataaaca atatagaaaa
     1021 atgtttgatg cttacccaga tggagaaata ggtaaaaatg ccattacaca ttataaagta
     1081 atagaaagaa tgaactatgt tactttagta agttgccagt tagaaacagg gcgaacacac
     1141 caaattagag tgcacatgaa atttattggc catactttat ttaatgattg ggagtatgga
     1201 ggagataaaa ttttaaaagg caccattttt accaaataca aacaatttgt agataattgt
     1261 tttgctattt gccctaggtg tgccttacat gctaaaacat tgggctttat acacccaact
     1321 acacaaaaag aaatgttttt tgaagcggaa cttcctgaag atatgaaaaa cgtaattagc
     1381 aaatggagag gctatgttgg aaacaaacaa ttagactaat acttgtgccc taacttactt
     1441 aaataataac tgtagcatta caataaaaga ttatcaatta atcttacttc gccaataaat
     1501 gctgcaatta gtacaactaa ttttacccct tctttatatt ctgcaatagg ttgtaaattt
     1561 tgagcattac aaatgcttat ataatcaatt ttcaaaaaac cattctttag taattcttct
     1621 tctgccctct tgattaagcc attaaaaggc aatctttcaa aattattttt catgtacagt
     1681 aagttattat aaatagctgc agccttagtt ctttcttctt ctgttagtct tctatttcta
     1741 ctgctcatgg ctaatccatt ttcttctctt atggtaggta caataactaa tttaagtttg
     1801 ttttgccaat tcattaactc aaccaatttt gatacaacca tacattgttg ataatctttt
     1861 tgacctaaaa acaatatagt gggtttaacc attagcagca atctttccac tacattacat
     1921 acaccttgaa aatgaccttt tctgtattta ccttccaaaa tttcttccaa ataactcaat
     1981 tcataatgtt ttaagttatc aatcccatcc gaataaattt catttacaga agggagaaaa
     2041 atgatatcac aaccagcttt ttctaataaa aaaatatcgt tttcaatggt aataggatat
     2101 ttttcaaaat cgtttttatc attgaactgt gtagggttta caaatatgct tgctactgtt
     2161 attgcacatt gttgtttgct ttcttctatc aaagctatat gtccattatg caaagctccc
     2221 attgttggca caaaaccaat atttatattg ttagtataat atttttttag cgtattttca
     2281 agtattgctg cagttttaca aataaccatt ttaaagcata ttatttatta caaaaagaag
     2341 aacaaaacta agtatatgct attactttcc accttcttaa ttcaaaagca aaatattgta
     2401 taaacaatat tattttagct tttttataaa gtattaatag ctaaaggtat tgtaacattg
     2461 caaagaacta tttattgata aattgagtgt acaaaataac atattatcat caccaataga
     2521 atatctaaaa ggagttggac ctttaagagc cgattggttg aaaaaagaat tgaatatttt
     2581 taccttcaac gatttattgc atcttttccc atacaggcat attgataaaa gtaccatcac
     2641 cgctattaat aatattgaaa caaatgcaga atatatacag gttgttggaa agcttaccaa
     2701 cttacaaata attggcgaaa ggaaagggaa aaggcttact gcaactttaa aagatgaaac
     2761 aggaagtatt gagttagttt ggtttcaggg aattagttgg attcaaaaaa ttttaacaga
     2821 aggaagtatc tatttggttt ttggaaaact ctcctacttt aacaacactc cacaaatcat
     2881 tcatgcagaa attgaattgt acgtaccact acaaaataat gagagaaaat attttgaacc
     2941 agtttatcca tcaacagaaa aactaaaagc caaaggtctt aatggcagac aaattgccaa
     3001 acttacgttt gcattatggc aacaaattac cattgaaagt attccagaaa atattccaga
     3061 aaacatttta accgaattaa atttattaaa tagatataaa gcatataaat ctattcatta
     3121 tcctgcaaac aaaatagaat acaaccaagc tttaaacaga ttaaagtttg aagaattttt
     3181 tatagcacag gtaagattaa acttagttaa aatacaaagg caccaatgta gcaaaggagt
     3241 aatatttgaa aaagtaggtg cattattcaa taatttttac agcaaccact taccttttga
     3301 attaaccaat gcacaaaaaa atgtattaaa acaaattaga caagatgctg ccaaaggcca
     3361 tcaaatgaat cgtttattac aaggcgatgt tggaagtgga aaaacaattg tagcattact
     3421 tagtatgtta ttagcagcag ataatggtta tcaaagttgt ttaatggcac caacagaaat
     3481 tttagctatg cagcactaca atagtttaaa cagcttatta caaccaatga atatatctgt
     3541 aagtttatta acaggtagta caaaagccaa agaaagaaaa gcaattttaa ccgatttaca
     3601 agaaggaa
//
LOCUS       DAOQHP010000231         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16416_length_3584_cov_3.164636, whole genome shotgun sequence.
ACCESSION   DAOQHP010000231 DAOQHP010000000
VERSION     DAOQHP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16416_length_3584_cov_3.164636"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3584)
                     /locus_tag="PLY81_07700"
     CDS             complement(<1..>3584)
                     /locus_tag="PLY81_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNF30146.1"
                     /translation="QQVTLYTSVANGTLPNNGFIEFLGIKNDGIPDKPLYREASFQLS
                     DKISLQTDTACYFLTVNTVASQNYRYSNGINNVAGNSLAPEPFFIYTKQFDFKESIHR
                     GHGENAGERVTSSSYDIGEGWGSNEIYASSPYTFSTSNLYPYSTGPNASIKMAVNGVS
                     YLNRNVTLKLNNTAYINNQLLTAQQSAVFSSSVPMTAINNSTNDFSISINPVNIADNV
                     NRIYSAYVSLTYSRLFNFGNQTNFDFTIAASANPAGTYIEINNFNSAAIPVLYDLVNN
                     IKYTAVVENGIIKFALPFSSTSRNFVLVSQDISAIQTINNFQEKTFINYSLTSNQGNY
                     LIVSNKIAGINAGEPVDQYRQYRTSLAGGGFNAKIYDIDDLVDQFAFGIKKHPLSVKN
                     FVRFAKQNFTTNPKQIFIIGKAVAYDEYAVNQSNLNADKLNLVPTFGWPASDALLVSD
                     GVNPQPLLLVGRLSVVNPNEVSIYLQKVQQYEQEAANNVQTVANKLWQKQIVHVAGGN
                     DPNIEPLLIAYLNGYKAIIKDTLFGGSVADFNTVATGGGATPAVVDLLKNYFKNGIAL
                     LTYFGHSAATQLDYNLNNPLDYNNPGKYPMFLLNGCNAGNFFDYDESRLNNITSFSEK
                     FVFANQHGAIGVIASSHFGLTGYLNSYSTGFYNSIRTSTGYNNYVGKNMMDAMAPFSF
                     NDFFSRIHAEQFILHGDPAIKVYAKDKPDFAVEENSVTINPTVLSVADNNFTLKAKLF
                     NIGKSQAAKTVNAGDSVHVIVKWQHGNGSVDYLFNGFIKPSIRNTDSLILNASISPLR
                     DKGNNCITIVIDSLNQYNELSKLNNTVSKCFFIFDDDIKPVYPYNFSIVNTNTIKFFA
                     TTANPIAAAKDYVMEIDTTELFNSALKITHFKNSVGGAIEFNPSIALTDSTVYYWRVA
                     AVPNSGETRWNNASFVYLNGTETGYNQSHLYQHFKSSFNRIYIDSFSRNWNYGLAATN
                     LNITQGVFPYSPYDADYSVSKNGKYVVSSGCLGYAVRFTLFDATTMKPYYNQALPALD
                     SVGASGGFMGSSAVNCAKPGRQYNFEFSYNSLLERNKMRDFINWIPNNVVAVARVNLD
                     LPFDANPVAVWMNDSIANGSIQNTLYGKLKENGFLNIDSFNFPRSWIFAFQKGNAAFT
                     PKSAFGFDINDIAHLDVTVNSTDSLGYVTSPILGPAKQWKTLKWRGKT"
BASE COUNT         1249 a          602 c          539 g         1194 t
ORIGIN      
        1 tgttttacct cgccatttta atgttttcca ttgttttgct ggtcctaaaa ttggagaagt
       61 aacatagcct aaagaatcgg tggaattaac tgttacatct aaatgagcaa tatcgttaat
      121 atcaaaacca aaagcagatt ttggagtgaa agcagcattg cctttttgaa aagcaaaaat
      181 ccatgaacga ggaaaattaa atgaatcaat atttaaaaag ccattctctt ttaacttgcc
      241 ataaagagtg ttttgaatag agccattagc tatagaatca ttcatccaaa ctgcaacagg
      301 attagcatca aaaggtaaat ctaagttcac tcttgcaaca gccactacat tatttggaat
      361 ccagttgata aaatctctca tcttatttct ttccagtaaa gagttataac taaattcaaa
      421 attatactgc ctacctggct ttgcacagtt tacagcactg ctacccataa aacctccaga
      481 agcaccaaca gaatctaatg ctggtaatgc ttggttataa tatggtttca tggtagttgc
      541 atcaaacaaa gtaaaacgaa cagcataacc taagcaccca cttgatacaa catattttcc
      601 attttttgaa acagaataat ccgcatcgta tggagagtaa ggaaatactc cttgtgtaat
      661 attcaagtta gttgctgcca atccataatt ccaatttctt gagaaactat ctatatatat
      721 tctattaaaa ctgcttttaa aatgttggta taaatgcgat tgattatagc cagtttctgt
      781 tccatttaaa tacacaaaac ttgcattatt ccatcttgtt tccccactat taggtactgc
      841 agcaacacgc caatagtaaa ctgttgaatc agttaaggct atagatggat taaattcaat
      901 agccccacca accgaatttt taaaatgggt aatttttaaa gcagaattaa acaactccgt
      961 agtatctatt tccatcacat aatcttttgc agcagcaatt gggtttgcag tagtggcaaa
     1021 aaattttatt gtattggtat ttactataga aaaattataa ggataaacag gtttaatatc
     1081 atcatcaaaa ataaaaaagc atttgcttac agtattattt agtttactca attcattgta
     1141 ctggtttaaa ctatctataa ctatggtaat acaattattt cctttatctc ttaaaggaga
     1201 aatacttgca tttaatatta aagaatcggt attacgaata gagggtttaa taaaaccatt
     1261 aaataaataa tctacacttc cattaccatg ttgccatttt acaataacat gaacagagtc
     1321 gccggcattt acagttttag cagcctgaga ttttccaata ttaaaaagtt tggctttaag
     1381 tgtgaaattg ttatcagcaa ccgaaagaac tgttgggttt atagttacac tattttcttc
     1441 tacagcaaaa tctggtttat cttttgcata aacttttatg gctggatctc catgtaaaat
     1501 aaattgctca gcgtgaatac gagaaaaaaa atcgttgaaa gaaaaaggag ccatggcatc
     1561 catcatattt tttccaacat aattattgta ccctgtagaa gttcttatgg aattataaaa
     1621 accagtagag taagagttta aataacctgt tagcccaaag tgagagctgg caattactcc
     1681 aattgcacca tgctggtttg caaatacaaa tttttctgag aatgaagtaa tattatttaa
     1741 tctagattca tcataatcaa aaaagttacc tgcattgcac ccatttaata aaaacattgg
     1801 atattttcct gggttgttat aatctaatgg attattcaaa ttataatcta gttgtgtagc
     1861 agcactatgc ccaaaataag ttagtaaggc tataccattt ttgaagtaat tttttaataa
     1921 atcaactact gctggagttg caccaccacc ggttgcaaca gtattaaaat ctgctacact
     1981 accaccaaac aaagtatctt taataatagc tttataacca tttaaataag caattaataa
     2041 aggttcaata ttaggatcgt tgccacctgc aacatgtact atttgttttt gccaaagctt
     2101 attagctaca gtttgtacat tgttggcagc ttcttgttcg tattgttgta ccttttgtaa
     2161 ataaatgctt acttcattag ggtttactac agataaccta cctaccaaaa gtaaaggttg
     2221 cggatttaca ccatccgaaa ccaataatgc atctgaagca ggccagccaa acgttggaac
     2281 taaatttagt ttatctgcat taaggttact ttggttaact gcatattcat catacgcaac
     2341 tgctttacca ataataaata tttgtttagg gttagtggta aaattttgct tggcaaaacg
     2401 tacaaaattt tttacactta aagggtgttt tttaattccg aaagcaaact gatctactag
     2461 gtcatctata tcatatattt tagcattaaa accaccgcca gcaaggcttg tacgatactg
     2521 acgatattga tctacaggct ctccagcatt aatgcctgca attttattag atacaattaa
     2581 ataatttcct tgattactag ttaagctata attgataaat gttttctctt gaaaattatt
     2641 tattgtttgt atagctgaaa tatcctgact tactaaaaca aaatttcttg atgtagaaga
     2701 gaaaggcaat gcaaatttta taataccgtt ttctacaaca gcagtatatt taatattatt
     2761 tactaaatcg tacaaaacag gaatagctgc actattaaaa ttatttatct ctatataagt
     2821 accagcaggg ttggcacttg cagcaatggt aaaatcaaaa ttagtttgat ttccaaagtt
     2881 aaatagcctt gaataggtta aacttacata agccgaataa attctgttaa cattatctgc
     2941 tatattaact gggttaatag aaatgctaaa atcgtttgta gaattattta ttgcagtcat
     3001 aggaacacta ctactaaaaa cagcagattg ttgtgccgtt agcaactgat tatttatgta
     3061 agcagtatta ttgagtttta atgtaacatt tctgtttaaa taagaaacgc cattaactgc
     3121 catttttata cttgcattag gacctgttga ataaggatat aaattggaag tagaaaaagt
     3181 atagggcgaa cttgcatata tttcatttga accccatcct tcaccaatat cataagaaga
     3241 agaggttact ctttctcctg cattttctcc atgacctcta tgaatactct ctttaaaatc
     3301 aaattgtttt gtataaataa aaaaaggctc aggggctagt gaatttccag ctacattatt
     3361 aatgccattg gagtaacgat aattttgaga agcaacggta tttactgtta aaaaataaca
     3421 agcagtatct gtttgtaaac taattttatc ggagagttga aaactagcct ccctatacaa
     3481 aggtttatca ggaattccat cgttttttat gcccaaaaac tcaataaaac cattattagg
     3541 caaagttcca ttagcaacac ttgtatataa agtaacttgt tgcc
//
LOCUS       DAOQHP010000232         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16730_length_3541_cov_3.475330, whole genome shotgun sequence.
ACCESSION   DAOQHP010000232 DAOQHP010000000
VERSION     DAOQHP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16730_length_3541_cov_3.475330"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLY81_07705"
     CDS             <1..735
                     /locus_tag="PLY81_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HNF30147.1"
                     /translation="AKYETVLLTDADCLPASEDWMYYMQDAYNDDIEVVLGYGAFKKR
                     KGILNKLIRFETFHTALQYFSYALAGIPYMGVGRNLSYKKDIFFRNKGFSSINMIPSG
                     DDDLFINKVTNKKNTAIVIDPLAHTLSEPKKTWDEWMTQKYRHYTTAKFYKPIHKFLL
                     GLYSFSLFCIYPLLIISAVFYCWWLTLSVFAIRFIVQAIIMFKTMKKLNEKDLFPWFI
                     LLDLWMPLYYLFTIPAIWKSPKRTWG"
     gene            755..1384
                     /gene="rsmG"
                     /locus_tag="PLY81_07710"
     CDS             755..1384
                     /gene="rsmG"
                     /locus_tag="PLY81_07710"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNF30148.1"
                     /translation="MDIIKKYFDDFTPKQIEQFAALKNLYKEWNEKINVISRKDIDEI
                     YLHHVLHSLSIAAIGNFVPQTQVIDIGCGGGFPGVPLAIFYPDVHFHLVDSIGKKLKV
                     VNAICEAIEVKNITTQHTRVEEIKNKKFDFAISRAVAPLKELWHWSNPIIKKAVLPNQ
                     EMANGLICLKGGDLHQEIFESQTKPSMMGIYNIFKEEYFKEKFILYVKK"
     gene            complement(1418..2953)
                     /locus_tag="PLY81_07715"
     CDS             complement(1418..2953)
                     /locus_tag="PLY81_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30149.1"
                     /translation="MKKIYSISLLIIVALKLNAQVPDDALRTAWYTFNGSARNMAIGG
                     VMGSLGGDITAANINPAGLGLFKTKEFILSPGFNLNNNNLLFRGTDTTDKKNSFNYGT
                     IGFVFGKPNNPNYSKFTSSAFSISVNQLANYNNSIQFKGFNNFSSFSEQYLEELIADG
                     ANMTAAEQNYIYGSSLAFRTYLIDTIADANGNLIGFQSLVPISTGVNQTYNEKTRGGL
                     HEIALGFASNLEDKLYIGGSVNIPILSFQRDFTYSENDATNNTNNDFDNFIYTEKFKS
                     FGAGFGAKLGFIYKPQERWRLGFAFHTPQFMFFKDKIRTWMTTNTEAYAGIKSESSDA
                     LNSGNAGERKYNLVTPWRALVSASYVFNEVKDVRKQRAFISADIEFVNYRGARFARSE
                     EDDYVAKAYYKTVNATVKDYYKGNFNFRVGGELKLNTFMFRLGAAYYGSPYKERVLNA
                     NKMMLTGGIGYRNYGFFIDLAYAHSFNKDVVFPYRLNDKPNTFAEQTGSRGNIMLTFG
                     VKL"
     gene            complement(3014..>3541)
                     /locus_tag="PLY81_07720"
     CDS             complement(3014..>3541)
                     /locus_tag="PLY81_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30150.1"
                     /translation="RLTYTNCYTNYPYNGVNNWNSYYYNNYNSWNNHIYYNSPCIGSN
                     WYSFNYNPWGSWNSPFYYMVPYKNPTVYTGNTKASYVTAYTTKSYSNTNVKTGIKAGT
                     SSTNRNTGFGKLVKRVFSTNGNTGSGNAGWDRPARTYTNSSGTTTSGSSSAGGRSGGY
                     KSTGSSSSSSRSGRN"
BASE COUNT         1176 a          572 c          521 g         1272 t
ORIGIN      
        1 gctaaatacg aaactgtttt attaacagat gcagattgtt taccagccag tgaagattgg
       61 atgtattata tgcaagatgc ttataatgat gatattgaag ttgttttagg ttatggtgct
      121 tttaaaaaaa gaaaaggtat tttaaacaaa ctcattaggt ttgaaacttt tcatacggca
      181 ttacaatatt tctcttatgc tttggctggc ataccataca tgggtgtagg tagaaattta
      241 agttataaaa aagatatttt ttttaggaat aaagggttct cttccatcaa catgatacca
      301 agtggcgatg atgatttatt tattaataag gttaccaata aaaagaatac tgctattgtt
      361 atagatcctt tggcacacac ccttagcgag cccaaaaaaa catgggatga atggatgaca
      421 caaaaataca ggcattatac aacagccaag ttttataaac ctatacataa atttttatta
      481 ggcttatatt catttagctt attttgtatt tatccattat taataataag tgctgttttt
      541 tattgttggt ggttaactct atctgtattt gctataaggt ttatagtgca ggcaattatt
      601 atgtttaaaa caatgaagaa gttgaacgaa aaagacttat ttccttggtt tatattattg
      661 gatttgtgga tgcctttata ttatttattc actattcctg ccatttggaa aagccctaaa
      721 agaacttggg gttaattact attgattttg tacaatggat ataataaaaa aatatttcga
      781 tgattttact ccaaaacaaa ttgaacaatt tgctgcacta aaaaatttgt acaaagagtg
      841 gaatgaaaaa ataaatgtta tttcaagaaa agatatagat gaaatttatt tgcatcatgt
      901 attacactct ttaagtattg cagcaattgg caattttgtg ccacaaaccc aagttattga
      961 tataggctgt ggtggtggtt ttcctggtgt tcctttagca attttttatc cagatgttca
     1021 ttttcattta gtggatagta ttggtaaaaa attgaaagtt gtaaatgcaa tttgcgaagc
     1081 tattgaagta aaaaatatta ctacacaaca taccagagtg gaagaaataa aaaacaaaaa
     1141 atttgatttt gctattagcc gtgcagttgc tccattaaaa gaattgtggc attggagtaa
     1201 ccccattatt aaaaaagcag ttttaccaaa tcaagaaatg gctaacggat taatttgttt
     1261 aaaaggtggc gacttgcatc aggaaatttt tgaaagccaa accaaaccct ctatgatggg
     1321 tatttacaat atttttaaag aagaatattt taaagaaaag tttattttgt atgttaagaa
     1381 gtaaactatg cttctatttt tatcaataaa taacttatta caatttaact ccaaaagtta
     1441 gcataatatt tcctctactt cctgtttgct ctgcaaatgt gttaggctta tcgtttaaac
     1501 gataaggaaa aacaacatct ttattaaagc tatgagcata agctaaatca ataaaaaagc
     1561 cataatttct ataccctatt ccaccagtta acatcatttt atttgcattt aaaacacgtt
     1621 ctttataagg gcttccataa taagcagctc ctaacctaaa cataaatgta tttaacttta
     1681 gctcgccgcc tactctaaaa ttaaaatttc ctttgtaata atcttttaca gttgcgttta
     1741 cagttttgta atatgcctta gctacgtaat catcttcttc acttcttgca aatctagcac
     1801 cacggtaatt aacaaattca atatctgcac ttataaatgc cctttgcttg cgtacatctt
     1861 tcacttcatt aaaaacatag cttgcactta ctaatgctct ccaaggggtt actaaattat
     1921 actttctttc tcctgcattg ccactattta atgcatcgct gctttcactt ttaatacctg
     1981 cataagcttc ggtattagtt gtcatccaag ttctaatttt gtctttaaaa aacataaact
     2041 gaggtgtatg aaatgcaaaa cctaaacgcc agcgttcttg tggtttatat ataaaaccaa
     2101 gtttagcacc aaagcctgca ccaaagcttt taaatttttc ggtataaata aaattatcaa
     2161 aatcgttatt ggtattgtta gttgcatcat tttcggagta agtaaaatct ctttgaaaag
     2221 ataaaatggg aatattaaca ctaccaccaa tatataattt atcttctaag ttacttgcaa
     2281 agcccaacgc aatttcgtgt aatccacctc tagttttttc attatacgtt tgattaactc
     2341 cagtagagat tggcaccaaa ctttgaaatc caattaagtt accatttgca tctgcaattg
     2401 tatcaatcaa ataagttcta aatgctaaag aagatccata aatatagttt tgttctgcag
     2461 cagtcatgtt agcaccatct gcaattaatt cttccaaata ttgttctgaa aaagaactga
     2521 aattattaaa gcctttaaat tgaatactat tgttgtagtt tgctaactga ttaactgata
     2581 tggaaaatgc actacttgta aacttactgt aattggggtt atttggcttg ccaaatacaa
     2641 agcctatagt accataatta aaactatttt ttttatctgt ggtatctgtt cctctgaata
     2701 ataaattatt attgtttaaa ttaaaaccag gcgataaaat aaattctttt gttttaaata
     2761 agcctaagcc tgctgggtta atattagctg cagtaatatc tcctcctaaa ctgcccatta
     2821 cacctccaat agccatgttt cttgcacttc cattaaaagt gtaccatgct gttctaagtg
     2881 catcgtctgg cacttgtgca tttagtttta aagcaacaat tatgagtaaa cttattgaat
     2941 atattttttt cattagaaat gaaataaaag ttttttaata tttgggttga accactcttg
     3001 cagcagttat attttaattt ctaccacttc tgctactact gctactgcta cctgtacttt
     3061 tgtaacctcc acttctacca cctgcactac tactgccaga agttgttgta ccagaactat
     3121 tagtataagt tcttgctggt ctatcccatc ctgcattgcc actaccagta ttaccatttg
     3181 tagaaaatac tcttttaact aattttccaa aacctgtatt tctgtttgta gaactagtac
     3241 ctgctttaat accagttttt acattggtat tactatagct ttttgttgta taggcagtaa
     3301 catagcttgc ttttgtattg cctgtatata cagtaggatt tttgtatgga accatataat
     3361 aaaaagggct gttccaacta ccccaaggat tgtaattaaa gctataccaa ttgctgccaa
     3421 tgcaaggtga attatagtat atatggttat tccaactatt ataattatta tagtaataac
     3481 tattccaatt attaacgcca ttgtatggat aatttgtgta acaattagta taagttagtc
     3541 t
//
LOCUS       DAOQHP010000233         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16890_length_3523_cov_3.845444, whole genome shotgun sequence.
ACCESSION   DAOQHP010000233 DAOQHP010000000
VERSION     DAOQHP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16890_length_3523_cov_3.845444"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLY81_07725"
     CDS             <1..203
                     /locus_tag="PLY81_07725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614091.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNF30151.1"
                     /translation="KAEYDRIVSDAQTAINQQKNAALTDVKNLVGTLVIEVSEKILRR
                     ELSNKAEQENYIKQLAEEVKLN"
     gene            239..802
                     /gene="atpH"
                     /locus_tag="PLY81_07730"
     CDS             239..802
                     /gene="atpH"
                     /locus_tag="PLY81_07730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNF30152.1"
                     /translation="MQNPRLAHRYAKSLMDLSIEKKQLEEVCNDMKYLQALCSQSREF
                     TSLLKSPIIKADKKRNIINAVTEGKVSEITKLFNQLLVTKGRESSLAEIANAFIEEYN
                     TLKGIHKVVLTTAVELSKDVLNTIGNKIKTERGIDNIELEAKVNKDIIGGFILEFDNN
                     LVDASIVRDLRDVKKQFAKNIYIPEIR"
     gene            839..2416
                     /gene="atpA"
                     /locus_tag="PLY81_07735"
     CDS             839..2416
                     /gene="atpA"
                     /locus_tag="PLY81_07735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNF30153.1"
                     /translation="MVNIKPDEISAILRQQLSNFNASADLEEVGTVLQVGDGIARVYG
                     LNNVRSGELVEFENGVRAIALNLEEDNVGVVLMGESGDIKEGAKVRRTGQIASIKVGE
                     GVVGRVINTLGEPIDGKGPIHGELYEMPLERKAPGVIYREPVTEPLQTGIKSIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFFDAGKPVYCIYVAIGQKASTVAGVMKT
                     LADNGAMAYTTIVAASASDPAPQQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLKRPPGREAYPGDVFYLHSRLLERAAKVINNDSVAKNMNDLPESIKHLVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEGNLFNAGIRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQALYRELEAFSKFGGDLDAATKNVIDKGARNVEILKQNQY
                     TPFSVEKQIAIIYLGTQGLLKDVAVKNVKAFEEMFLTEMQNKLPEVLAEFKKGQLNND
                     SIDKMVNLAKGLIPQYK"
     gene            2547..>3523
                     /locus_tag="PLY81_07740"
     CDS             2547..>3523
                     /locus_tag="PLY81_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30154.1"
                     /translation="MKPYWNIKITVRVYILVLTIFTFFRIILFINEYGRLDETATLTN
                     RLLAFFMGVRFDIVISGYILFFPFVALSILNITKDSLLVKRIIFYFIFILFTLAFLVC
                     AADIPYFNQFFSRFSVTAFEWLNSPMFVLKMIIQEPRYWLFFILFVVVVIIFFKRLQH
                     IFKSNELTIIKNKKWKFGLISLLAAGFIFLGVRGRIDEKSPIRVGTAYFCNNAFLNQL
                     GLNPNFTLMRSWIDAMEEENKLVNLMDEKIAIQNTQKYLQITNPSQTLPISRIENDTV
                     TNKHNYNVVFIIMESMSAAKMKRHGNAKNLTPFLDSISTKGLYFENNYSA"
BASE COUNT         1283 a          459 c          639 g         1142 t
ORIGIN      
        1 caaaagcaga gtatgataga atagtttctg atgctcaaac tgccattaac caacaaaaaa
       61 atgcagcttt aaccgatgta aaaaaccttg ttggcacatt ggtaatagag gttagcgaaa
      121 aaatattaag aagagaatta agcaataaag cagagcaaga aaattatatt aaacaattag
      181 ccgaggaggt aaaattaaat taagcagtaa ctgctaattc ttcattatta attgagttat
      241 gcaaaatcca cgtttagcac ataggtacgc aaaaagttta atggatttat ccattgaaaa
      301 aaaacagtta gaagaggttt gtaatgatat gaaatatttg caagcacttt gctctcaaag
      361 tcgtgagttt accagtttat taaaaagccc aattataaaa gcagataaaa aaagaaatat
      421 tattaatgca gttaccgaag gaaaggtgag cgaaattact aaattgttta accagctttt
      481 agttactaaa ggaagagaga gcagccttgc cgaaattgca aatgctttta ttgaagaata
      541 caacacatta aaaggtattc ataaagtggt acttaccaca gcagttgaat taagtaaaga
      601 tgttttaaat acaattggca ataaaataaa aacagaaaga ggaattgata atattgaatt
      661 agaagcaaaa gtaaataaag acattattgg aggatttatt ttagaatttg ataataacct
      721 tgttgatgca agcatagtaa gagacctaag agacgtgaag aagcagtttg caaaaaatat
      781 ttatattcct gaaattagat aagcacaaat taattttaaa tttttataat tctattatat
      841 ggtaaacatt aaacccgatg aaatatctgc cattttaagg cagcaactta gcaactttaa
      901 tgctagtgca gatttggaag aagtaggcac agtattacaa gttggtgatg gtattgcacg
      961 tgtatatggt ttaaataatg tacgcagtgg tgagttggtt gaatttgaaa atggtgtaag
     1021 agcaattgcc ttaaacttag aagaagataa tgtaggtgtg gttttgatgg gagaaagtgg
     1081 agacattaaa gaaggtgcca aagttcgtag aactgggcaa attgcttcta ttaaagtagg
     1141 agaaggtgta gtaggccgtg taatcaatac tttaggagaa ccaatagatg gcaagggccc
     1201 aattcatgga gaattatatg aaatgccttt agagcgtaaa gcacctggcg taatttatcg
     1261 tgagccagta acagaaccat tacaaactgg tatcaaatct atagatgcaa tgattcctat
     1321 tggtcgtggc caacgtgagt taattattgg tgatagacaa acaggtaaaa ctgcaattgc
     1381 tattgataca atcatcaatc aaaaagaatt ttttgatgca ggcaaaccag tttattgcat
     1441 ttatgttgca ataggacaaa aagcaagtac tgttgctggt gttatgaaaa cattagcaga
     1501 taatggtgca atggcttata caacaatagt tgcagcttct gcatctgacc ctgctcctca
     1561 acaattctat gctccatttg caggtgctgc tattggagaa ttttttagag atactggtcg
     1621 tccggcatta attatttatg acgatttatc taaacaagct gtagcatatc gtgaggtttc
     1681 tttattatta aaaagacctc caggacgtga ggcttaccct ggtgatgtat tctatttaca
     1741 tagccgttta ttagaacgtg cagcaaaagt tattaataac gattcggttg caaaaaacat
     1801 gaacgattta ccagaaagca ttaaacattt agtaaaaggt ggaggtagct taactgcttt
     1861 accaattatt gaaacacagg ctggcgacgt atctgcatac attccaacaa acgtaatttc
     1921 aattaccgat ggacaaattt tcttagaagg taacttattt aatgctggta ttcgtcctgc
     1981 aattaacgta ggtatttctg taagtcgtgt aggtggtaat gcacaaatta aatcaatgaa
     2041 aaaagtagct ggtactttaa aattagacca ggcgttatac cgtgagttag aagccttctc
     2101 taaatttggt ggagatttag atgcagctac caaaaatgta attgataaag gtgcaagaaa
     2161 cgtggagatt ttaaaacaaa atcagtacac accattctcg gtagaaaagc aaattgctat
     2221 tatttactta ggtacacaag gtttgttgaa agatgtagca gtaaaaaatg taaaagcttt
     2281 tgaagaaatg tttttaactg aaatgcaaaa caaattacca gaagtgttgg ctgaatttaa
     2341 aaaaggacaa ttaaataatg acagtattga taaaatggta aacttagcaa agggcttaat
     2401 accacaatat aaataatttt aaatttttat attctcaaaa tcggcttcaa taacttgaag
     2461 ccgattttgt ttatgaaaca agttttttaa aaaaaagaat atttttacta cttaattaat
     2521 aatattttct ataaataatt acattaatga agccttattg gaatattaaa ataactgtta
     2581 gggtttatat tttagtacta acaattttta ctttttttag aattattttg tttataaatg
     2641 aatatggcag attagatgaa actgcaactt taaccaatag gttactagct ttttttatgg
     2701 gtgtaagatt tgatattgtt ataagtggtt atatactatt tttccctttt gtagctttat
     2761 caatattaaa tattacaaaa gatagcctac ttgtaaagag aattattttc tattttattt
     2821 ttatattatt tacgttggca tttttagttt gtgctgccga tattccttat tttaatcaat
     2881 tcttttctcg tttttctgta acagcttttg agtggttaaa cagcccgatg tttgttttaa
     2941 aaatgataat tcaggagcca cgttattggc tattttttat tttgtttgtg gtagtggtaa
     3001 ttattttttt taaaaggctt caacatattt ttaaatcaaa tgagttaacc attattaaaa
     3061 ataaaaaatg gaaatttgga ttaatttctt tgcttgctgc tggctttatt tttttaggtg
     3121 ttaggggtag gatagatgag aaatctccta ttagagttgg tacagcttat ttctgtaata
     3181 atgctttttt aaatcagtta gggttaaacc ccaattttac tttaatgaga agctggattg
     3241 atgcaatgga agaagaaaat aaattggtga acttaatgga tgaaaaaatt gctattcaaa
     3301 acacacaaaa atatttgcaa ataactaacc catcgcaaac attacccatt agtagaatag
     3361 aaaatgacac agtaaccaac aaacataact ataatgttgt atttattatt atggaaagca
     3421 tgagtgctgc aaaaatgaaa agacatggca atgctaaaaa cctcactcca tttttagata
     3481 gcatttctac taaaggctta tattttgaaa ataattactc tgc
//
LOCUS       DAOQHP010000234         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16908_length_3520_cov_3.326118, whole genome shotgun sequence.
ACCESSION   DAOQHP010000234 DAOQHP010000000
VERSION     DAOQHP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16908_length_3520_cov_3.326118"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1144)
                     /locus_tag="PLY81_07745"
     CDS             complement(245..1144)
                     /locus_tag="PLY81_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF30155.1"
                     /translation="MKWNRLKAHAIVLAGNFFFGVSVIAIKHLTPGLMPPIALNVVRV
                     CIALCLFWILWLFKPVKPGIQKKDIPLFILCAATGVAINQILFVQGTSLTSPIHAALL
                     MLVTPIVITILAVWFFKEKITSNKLLGLLLGVLGAVILVVLKKHADKESSAFGDILII
                     INAISYAFYLILAKPLMIAYRPEQVIRWVFLFGTIIIVPFGFSSISHIQWAAFQWHHW
                     LALCFVVLGSTFLSYLFMVYGLAKLGSTVVGTYMYTQPIFATIAAMVLFNEKLTAIKL
                     LAAIFIFGGVYLVNKQQNKKQHL"
     gene            complement(1131..2261)
                     /locus_tag="PLY81_07750"
     CDS             complement(1131..2261)
                     /locus_tag="PLY81_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF30156.1"
                     /translation="MNKKFNIQTVLVAPLDWGLGHATRCIPIISALLKNNYTVILAAS
                     GKQKILLQQEFPEIKIIDLKGYNIQYSKTSFGLPFKIFLQIPKIVWAIYYEHKWLKKI
                     VNKHAIDCVISDNRYGLFSKKIPTVFITHQLTIKMPFLWLQSFVQKINYKFINQFTAC
                     WIPDVEGKDKMAGILSHPTKYPSMPIQYLGNLSRFEWQNTVEAYTYKYCILLSGPEPQ
                     RSILEKIIIKDLPNITQQVLLIRGLPNDNTVLPTNNNVIVKNHLQGVEICKALQQSEW
                     VICRSGYTTIMEILSLRKKAVLIPTPAQTEQEYLANFLMQKQWCYSVAQNKFVLSKER
                     NNIENFSFHVPVIGANNLQQTIPVLLNNLIIYSTNTTQYEVE"
     gene            2313..2435
                     /locus_tag="PLY81_07755"
     CDS             2313..2435
                     /locus_tag="PLY81_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30157.1"
                     /translation="MKTSIVKILLVVLVLSTVASSCSIFGKGRKDKSCMCPNYR"
     gene            2890..>3520
                     /locus_tag="PLY81_07760"
     CDS             2890..>3520
                     /locus_tag="PLY81_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30158.1"
                     /translation="MKKIYLLIPVLLFIKINLHAQLNYSFSATSSSYSAIVGGANPTL
                     LATTSGGKTDEGYANNISIGFTFNYNGTNYTTLGICTNGFIYLGGSLTNSSITYVNNI
                     ATEMAGVRPIIAPLWDDIDVQTETNIKYLVTGTAPNRVLTIEWANALWDPFAASAAIS
                     FQVKLYETSNIIEFVYKQEAGSIENIFGGVGASIGITNGNTGDGNYLSLN"
BASE COUNT         1278 a          534 c          524 g         1184 t
ORIGIN      
        1 cttttataac cgtgccacct gtaatagcag tgtaggtgcc acttgatgaa gaaaatgtgt
       61 aaccagaagc ctgtgcattt accttacaaa tactcataaa cactaaggta agtagtagta
      121 aaaactttct cataatgttg gttttggtta aaaataaatt attttttatt aaaatgtttt
      181 gcaatatacg agttattagt attaaaaaaa aaaattaatc cctacaaaaa aaattgcggg
      241 ctaattataa atgttgtttt ttattttgtt gcttattaac caaataaacc cctccaaaaa
      301 taaaaatggc agccaataat ttaatagccg taagtttctc gttaaacaaa accattgctg
      361 caatagtggc aaaaataggc tgtgtgtaca tataagtgcc aaccacagta gagcccaatt
      421 tagccaaacc atacaccata aacaaataag ataaaaaagt agagcctagt accacaaaac
      481 ataatgccaa ccaatgatgc cattgaaaag cagcccactg aatatggcta atactgctaa
      541 aaccaaaagg tacaataata attgtaccaa ataaaaatac ccaccttata acctgctctg
      601 gtctgtaagc aatcattaat ggctttgcca gtattaaata aaatgcataa gaaatagcat
      661 taatgattat taaaatatct ccaaaggcac tactttcttt atcggcatgt ttttttaaaa
      721 ccactaatat tacggcacct aatactccta aaagtaaacc caatagttta ttgcttgtaa
      781 ttttttcttt aaaaaaccaa acggccaaaa tagttattac aataggtgta accaacatta
      841 ataaagcagc atgtatgggg cttgttaaag aagtgccttg tacaaataat atttgattaa
      901 tggctacccc tgtagcagca caaagtataa ataaaggaat atcttttttt tgaatacctg
      961 gcttaacggg tttaaatagc cataaaatcc agaataaaca caaagcaata caaaccctaa
     1021 ctacatttaa agcaatgggt ggcattaagc cgggtgttaa atgttttatg gcaataaccg
     1081 aaacgccaaa aaagaaattt cctgctagta caatagcatg tgctttaagt ctattccact
     1141 tcatattgtg tagtattggt agaataaata attaaattat taagtaatac cggaatggtt
     1201 tgctgcaaat tattagcacc aataactgga acatgaaaag aaaaattttc gatattattc
     1261 ctctctttac ttaatacaaa tttattttga gcaacagaat aacaccattg tttttgcatt
     1321 aaaaaattgg ctaaatattc ttgctctgtt tgtgcaggtg tgggtattaa tacagctttt
     1381 tttctaagac ttaatatttc cataatggtt gtgtagccac tacggcaaat aacccactca
     1441 ctttgttgta aggctttaca aatttctacc ccttgtaaat gatttttaac tattacattg
     1501 ttattagttg gtaaaactgt attatcatta ggcaagccac gtattaataa tacttgttga
     1561 gtgatattgg gtaaatcttt tataataatt ttttctaaaa tagaacgctg tggctcgggc
     1621 ccagaaagta aaatacaata tttgtatgtg taggcttcaa cagtattttg ccattcaaac
     1681 ctagataaat tacccaagta ttgtataggc atggaaggat atttagtagg gtgagataaa
     1741 atgcctgcca tcttatcttt accttctaca tcgggtatcc aacaagccgt aaattggttt
     1801 ataaacttat aattaatttt ttgcacaaaa gattgcaacc atagaaaagg cattttaatg
     1861 gtaagctgat gagtaataaa aactgtgggt attttttttg aaaataaacc atatctatta
     1921 tcgcttatta cacaatctat ggcatgctta tttacaattt tttttagcca tttatgctcg
     1981 taataaatag cccatactat tttaggtatt tgtaaaaaaa ttttaaatgg taagccaaaa
     2041 gaagttttgc tgtactgaat attatagcct tttaagtcaa taatttttat ttctggaaac
     2101 tcttgttgta ataaaatttt ttgcttgcca ctggcagcaa gtattacagt ataattattt
     2161 ttgagtaaag cactaataat aggaatacac cttgtagcat ggcctaagcc ccaatctaaa
     2221 ggagctacca aaacagtttg tatgttaaat tttttattca acaggccata aaagtaatta
     2281 tttacaaatt aaatattaat ttacacgcca ttatgaaaac aagcattgta aaaatactat
     2341 tggttgtatt agttttaagt acagttgcaa gttcttgcag tatatttggt aaaggaagaa
     2401 aagacaaatc ttgcatgtgc cctaattaca gataatatat tgaagtaaat gctagggata
     2461 cttttttaca tttacattaa ttaaaacttg ttacagttta acacaattat ttgtaagtaa
     2521 aaatgcaacc taaagttgga tttttaaaaa aatatatagc ccccttaatg ttagttttgt
     2581 tggtagtagc aactcatcaa taaagtaaaa aacaaaaatc tttttatgct taaaaattat
     2641 tttccaattt gttttcttac aaattattat atcttttagt tgcttttatt gcttatctat
     2701 ttatctaaaa aacaccaact aattataaca aaacttgaat gcgtatttaa agaaccaaag
     2761 ctttaccatg ttaatgctgt atgcagtttg gaaacatctt attactttac attctaaaaa
     2821 aataatagct ttactaaata tttcaactaa taattcttta ataagtacat taattagaaa
     2881 attttaaaga tgaaaaaaat ttacctttta atacccgttt tattgtttat taaaataaac
     2941 cttcatgcac aacttaatta tagctttagt gccacaagca gtagttatag tgcaattgtt
     3001 ggtggtgcaa accctacgtt attagccact acatctggtg gtaaaacaga tgaaggttat
     3061 gccaacaata tatccattgg tttcactttt aattataacg gtactaatta taccacctta
     3121 ggtatttgta ctaatggttt tatttattta ggtggcagcc taaccaatag cagtataact
     3181 tatgttaata atattgcaac agagatggca ggagtgagac caattattgc acctttatgg
     3241 gatgatatag atgtacaaac agaaacaaat attaagtatt tagttacagg aactgcacct
     3301 aatagagtgt taactattga atgggctaat gccctatggg acccatttgc agcatctgca
     3361 gcaatttctt ttcaagtaaa gctatatgaa actagcaata taattgaatt tgtatataag
     3421 caagaagcag gtagtataga aaatattttt ggcggtgttg gtgcttctat tggaattaca
     3481 aatggcaata caggagatgg taattattta tcgttaaata
//
LOCUS       DAOQHP010000235         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_16991_length_3511_cov_4.131366, whole genome shotgun sequence.
ACCESSION   DAOQHP010000235 DAOQHP010000000
VERSION     DAOQHP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16991_length_3511_cov_4.131366"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLY81_07765"
     CDS             <1..255
                     /locus_tag="PLY81_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family competence protein"
                     /protein_id="HNF30159.1"
                     /translation="KSILIIDKRLETPLRNPKIMVDVIVLTNNPTIYLKDLHNTFHCN
                     QYVFDSSNPFWKIAYWKKDADSLHLQHHTTSTDGAFVMNL"
     gene            275..1795
                     /gene="purH"
                     /locus_tag="PLY81_07770"
     CDS             275..1795
                     /gene="purH"
                     /locus_tag="PLY81_07770"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNF30160.1"
                     /translation="MNKKIQTALISVFYKTGLETIAKKLHELGVTIYTTGGTQQFLND
                     INIPCIAVESVTNYPSILGGRVKTLHPLIFGGILGKRNDAQHLQDMQQYHIPIIDLVI
                     VDLYPFEETVANTTDENLIIEKIDIGGPSMIRAAAKNFNDVTVIASKNEYENFEKILT
                     NQQGEIFLEQRKAFAAKAFEIVMNYDIAIHNYFSPDNFILQSVTQKKILRYGENPHQE
                     AAFYGNLNNLFKQLNGKELSYNNLVDVDAAIQLMQEFKEVEESVFAIIKHTNVCGIAK
                     RKTVLESWNAALAGDNESAFGGILITNGTINKITAEAINELFFEVLIATAFEEEALQI
                     LRSKKNRILLEFNNKDTNQNNYQHKSLLNGVLKQQNDNHLIEEWKEVGGRTTTALEKE
                     DLAFANIISKHLKSNAIALVKNKQLVGKGCGQTSRIDALRQAINKAKQFNFSLKGAVL
                     ASDAFFPFNDCVSIAYKEGIEAFIQPGGSIRDKDSIEFAVQNNLAMVITSMRHFKH"
     gene            1807..2718
                     /locus_tag="PLY81_07775"
     CDS             1807..2718
                     /locus_tag="PLY81_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNF30161.1"
                     /translation="MTNKQKAYLALAVTSIVWGTTWVAVKFGVQKMPALQLAAIRLFF
                     AGLAFILFFTLVRKQSLPTKKQLQQLFFLSVFTFVLANALSAWSLQYIGSGLGALIGA
                     LYPLCVVIIEYFFYKNKSLNTTTLIGILLGFIGVGIVLYENTIDIHQEGFLIGILLSA
                     IATIAWSYSTIMIAKQKVKINPYYGLGWQMLFGSIMVLVLSLVTHNFIPISQIPIIAW
                     LMIAYLFIAGSIVAFIAFIYSMKHLSPTIASLYAYMNPIVAISIGTVLLDEKISIKLI
                     IGAIISLIGVYLVNYAAKKAMLKIDMD"
     gene            complement(2715..3281)
                     /gene="frr"
                     /locus_tag="PLY81_07780"
     CDS             complement(2715..3281)
                     /gene="frr"
                     /locus_tag="PLY81_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNF30162.1"
                     /translation="MLSEEIELVIDDTKSSMQKAINHLEQELLKIRAGKASPSMLDGI
                     MVDYYGNPTPINQAANISVLDAKTISIQPWEKSILAAIERAIMQANIGITPQNDGNQI
                     RLFLPPLTEERRKELFKKASGEGENTKVAIRNIRRDSIEQIKKLQKDGLSEDAAKDAE
                     AEIQTITDKFTALVGKHLEAKEKEMMSV"
BASE COUNT         1214 a          496 c          559 g         1242 t
ORIGIN      
        1 aaatccattc ttattattga taaaagattg gaaactccat taagaaaccc aaaaattatg
       61 gtagatgtaa ttgtattaac caataatccc accatatatt taaaagattt gcacaacact
      121 ttccactgta atcaatatgt ttttgattct tctaacccat tctggaaaat tgcttactgg
      181 aaaaaagatg cagacagttt acatttgcag catcatacaa cttcaactga tggggctttt
      241 gttatgaatc tttgattttg aaataactta atttatgaac aaaaaaattc agacagcact
      301 tatttctgtt ttttataaaa ccggtttaga aactattgca aaaaagctgc atgagttagg
      361 agtaactatt tataccactg gcggcaccca acaattttta aatgatatta atataccttg
      421 tattgcagta gaaagtgtaa ccaattatcc atctatctta ggaggcagag taaagacttt
      481 acaccctcta atttttggtg gcattttagg aaaaagaaat gatgctcagc atttgcaaga
      541 tatgcaacag taccatatac caattattga tttggtaatt gttgatttat atccttttga
      601 ggaaacagta gccaatacaa cagatgaaaa tttaattatt gaaaaaatag atattggtgg
      661 gccaagcatg ataagagctg cagctaaaaa ttttaatgat gtaacagtaa tagcctctaa
      721 aaacgaatat gaaaattttg aaaaaatttt aaccaatcaa caaggtgaaa ttttcttaga
      781 acaaagaaaa gcttttgctg caaaagcttt tgaaattgtt atgaattatg atatagcaat
      841 acataattat tttagtccgg acaatttcat tttacaatca gtaacacaaa agaaaatatt
      901 gcgttacggc gaaaacccac atcaagaagc tgctttctat ggtaatttaa ataatttatt
      961 taagcagtta aacggcaaag aactgtctta taataatttg gttgatgtag atgcagctat
     1021 acaattaatg caggagttta aagaagtgga ggaatcggtt tttgcaataa ttaaacacac
     1081 caatgtttgt ggcattgcaa aaagaaaaac agtacttgag agttggaatg ctgccttggc
     1141 tggcgataat gaaagtgctt ttggtggtat attaattacc aatggtacaa taaacaaaat
     1201 tacagccgaa gctataaatg aacttttctt tgaagtactt attgccacag cctttgaaga
     1261 agaagcttta caaattttaa gaagtaaaaa aaatagaata ttacttgagt ttaataataa
     1321 agacaccaat caaaataatt accaacataa gtctttatta aacggagttt taaaacaaca
     1381 aaacgataac catttaatag aagaatggaa agaggttggt ggaagaacaa ctactgcatt
     1441 agagaaagag gatttagcat ttgcaaatat tatttcaaag catttaaaaa gtaatgccat
     1501 tgcactggta aaaaataaac aattagtagg taagggctgt ggacaaacat caagaattga
     1561 tgcactaagg caagcaatta ataaagctaa acaatttaat tttagtttaa aaggtgcagt
     1621 actagctagc gatgcatttt ttccttttaa tgattgtgta agtattgctt acaaagaagg
     1681 tatagaggct tttatacaac caggtggtag tataagagat aaagatagta ttgaatttgc
     1741 ggttcaaaac aatctggcaa tggttataac aagtatgcgt cattttaaac attaacaaac
     1801 tttaatttga ctaataaaca aaaagcatac ttggcattag ctgttacatc aatagtttgg
     1861 ggtacaacat gggtggcagt aaaatttggt gttcaaaaaa tgcctgcctt acaacttgca
     1921 gcaatcagat tattttttgc tggcttagca tttattttat tttttacttt ggttcgtaag
     1981 caatccttac caactaaaaa acaattacaa caattatttt ttttatctgt ttttaccttt
     2041 gtgttagcta atgctttaag tgcatggagt ttacaataca ttggtagcgg attgggtgct
     2101 ttaattggtg cattatatcc tttatgtgta gttataattg aatacttttt ttataaaaat
     2161 aaatcattaa atacaactac attaattggc attttattgg gctttattgg cgtaggtatt
     2221 gtgttatatg aaaataccat agatattcat caagaaggtt ttttaatagg aattttatta
     2281 tctgcaatag caaccattgc atggagttat agcactataa tgattgcaaa acaaaaagta
     2341 aaaattaatc cttattatgg tttaggttgg caaatgcttt ttggttcaat aatggtattg
     2401 gtgctaagtt tagtaacaca taattttatt ccaataagcc aaattccaat tattgcctgg
     2461 ttaatgattg cttatttgtt tattgcaggt tctattgttg catttattgc cttcatttac
     2521 agtatgaagc atttaagccc tactattgct tctctatatg catatatgaa tccaattgta
     2581 gctatatcga ttggaactgt gttattagat gaaaaaattt caataaaact aattattggt
     2641 gcaataattt cattaattgg ggtttacctt gtaaattatg cagctaaaaa ggcaatgtta
     2701 aagattgata tggattaaac actcatcatt tctttttctt ttgcttctaa atgtttacct
     2761 accaatgctg taaatttatc agttatagtt tgtatttcag cttcggcatc tttagcagca
     2821 tcttcactta agccatcttt ttgtaatttc ttaatttgct caatagaatc tcttctaata
     2881 tttctaatag caactttggt attttctcct tcgccacttg cttttttaaa caattctttt
     2941 cttctttctt cggttagcgg aggtaaaaat aatctaattt gattgccatc attttgtggt
     3001 gttataccaa tattggcttg cataatagca cgttctatag cagctaaaat acttttctcc
     3061 catggttgaa tgctgattgt ttttgcatct aaaaccgaaa tatttgcagc ttgattaatt
     3121 ggggttgggt ttccataata atctaccatg attccatcta acatggatgg ggaggctttg
     3181 ccagctctta ttttcaataa ctcctgttct aagtgattaa ttgctttttg catagatgat
     3241 tttgtatcat caataactaa ttctatttct tctgataaca tggcttttat ttaatttggt
     3301 ggcaaatcta atgaatgaaa ctgaacaatt tttgttatta tttctttata caaaaacaat
     3361 ttcagtatat acaactgaaa ataataaata aaaaattagt aaatattagt tgataacaga
     3421 agtgccggtt gaagtttttt ctttttcttc aaaatctact ttaataactc tcattttact
     3481 aacttgagct ttgtttggcc tagttaaata a
//
LOCUS       DAOQHP010000236         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17031_length_3506_cov_3.416981, whole genome shotgun sequence.
ACCESSION   DAOQHP010000236 DAOQHP010000000
VERSION     DAOQHP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17031_length_3506_cov_3.416981"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..477)
                     /locus_tag="PLY81_07785"
     CDS             complement(139..477)
                     /locus_tag="PLY81_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF30163.1"
                     /translation="MQGSLPVSFTIDAINEIKRLISEEDFNASKKLRVGVKGGGCSGM
                     TYVLGFDEPTAEDTEYTTNDFSFIMNKSHSLYLIGMQIDWSDGLNSRGFTFTNPNVSK
                     TCGCGTSFAV"
     gene            641..1153
                     /locus_tag="PLY81_07790"
     CDS             641..1153
                     /locus_tag="PLY81_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30164.1"
                     /translation="MDSPIVIAFIIAIAVFFIGTLVVPYFRDKRKQKAKNSTNNIASK
                     QLQLQAYERLILLTDRIALPNLVPRLSQPGFTAKEMQLFIINNIIQEFEYNVTQQMYV
                     SNDAWTAVKNLKEQNIGITNQIGQLLPETATGLDLCRVLTEFLLKDKRGTLHELVSEA
                     ISFEAKKIMK"
     gene            complement(1156..1896)
                     /gene="aroE"
                     /locus_tag="PLY81_07795"
     CDS             complement(1156..1896)
                     /gene="aroE"
                     /locus_tag="PLY81_07795"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611044.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNF30165.1"
                     /translation="MKIFGLVGNSLSHSMSKKYFEDKFLKENLPDYYFKNFELTDINQ
                     LKELLATTKNLCGLAVTIPYKQAVLPFLNFATKEVETISACNCIKITDNVLYGFNTDI
                     IGFEKSFTSLLQPQHQKALILGNGGAAKAVKYVLQKLAIPYLIVSRNKEKQYPSISYQ
                     EINEQIVEEYKIIINTTPLGTFPAINNCANIPYNLLNKTNYLYDLVYNPAVTKFLELG
                     KQQGALIKNGSDMLIIQAEENWKIWNQN"
     gene            complement(1896..2672)
                     /locus_tag="PLY81_07800"
     CDS             complement(1896..2672)
                     /locus_tag="PLY81_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNF30166.1"
                     /translation="MNFNLTQMPDRTSKPRTNGLTMVMDKGLGLTDAKNFLEIATPHV
                     DLVKLGFGTSHVTPNLRKKIETYKAAGIHVYFGGTLFEAFLIRNQIDDYLAICKDYEI
                     DYIEVSDGSITIPHTEKCGYIEKMTKYATVLSEVGSKDATHIIPPYKWIELMRAELEA
                     GSSYVIAEAREAGNVGIYRGTGEVREGLVQEILTQIPSEKIIWEAPQKAQQLYFLELI
                     GCNVNLGNIAPTEILALEAMRVGLRGDTFRLYLDKEKAIY"
     gene            complement(2783..>3506)
                     /locus_tag="PLY81_07805"
     CDS             complement(2783..>3506)
                     /locus_tag="PLY81_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30167.1"
                     /translation="IVIDEKFDYAYRNMGDAYLRLKKYREAIEVLEKVLELTRPEDVI
                     YEAIGHSYHRMGNTGQARFHYKKAVHLNPDDSKLYYKIATTYMAENQWQQAIKQLEQA
                     LQIHKNVPEYNLAMGECKMKLNLFKDAVVYFSIVVNNKPKKIAGWEALIRCLFKATFY
                     DEAIEQCKAALKITENKPIFIFYYSTALLKVGKTKEGLLQLEKAMEKAPKLLKKFIEL
                     NPAILQNTAVVDIVAKYKKYNK"
BASE COUNT         1159 a          548 c          475 g         1324 t
ORIGIN      
        1 atgagctatg taaaaactgt tatagatgca cacaaaggaa gaattaaagt agaaagtact
       61 ttaggaaaag gaagcacttt ttgggtggag ttaccaagaa cttatacttc aaaaaagtaa
      121 ctgattttta ttatttcttt acactgcaaa tgaagtacca caaccacagg ttttacttac
      181 attggggtta gtaaaagtaa accctctgct atttaaacca tcactccaat ctatttgcat
      241 tcctattaaa tacaaactat ggcttttgtt cataataaaa gaaaaatcgt tggttgtgta
      301 ttccgtatct tctgcagtag gttcatcaaa tcctaaaaca taagtcatac cactacagcc
      361 tcctcctttt actccaactc tcaatttttt agaagcatta aaatcctctt cactaattaa
      421 tctttttatt tcattaatgg catctatggt aaaactaact ggcaatgaac cttgcataac
      481 ttgtaatatt tttacaaaag taaaaagtaa ataataaact agtataaaaa gctattacga
      541 taattggaat aagaaatgtt attttttaag gttttgaata aactatacta aattcaattt
      601 tttatggttt gctaaaaact atatttgcaa aaatttatat atggatagcc ctattgtaat
      661 tgcctttatt attgccatag ctgttttttt tattggtaca cttgtagtac catattttag
      721 agataaaaga aaacaaaaag caaaaaattc taccaataat attgcctcaa agcaactgca
      781 attacaagct tacgaaagac taattttatt aaccgataga attgcattac ctaatttagt
      841 accaagatta tcgcagcctg gatttactgc aaaagaaatg cagttattta ttattaataa
      901 tattattcaa gaatttgaat ataatgttac ccaacaaatg tatgttagca atgatgcttg
      961 gactgctgta aagaatttaa aagaacaaaa tattggtatt actaaccaaa taggacaatt
     1021 attaccagaa actgctacag gattagattt gtgtagagta ttaaccgagt ttttactgaa
     1081 agataaaaga ggaactttac atgaattagt gagtgaagca ataagttttg aagccaaaaa
     1141 aattatgaag taagtttaat tttggttcca aattttccaa ttttcttctg cttgaatgat
     1201 tagcatatcg ctaccatttt ttattaacgc accttgctgt tttcctaatt ctaaaaattt
     1261 tgtaacagca gggttgtaca ctaaatcata taaataattt gttttgttaa gtaggttata
     1321 cggaatattt gcacaattat ttatagcagg aaaagtacct aatggagtag tattaataat
     1381 aattttatac tcttcaacta tttgttcatt aatttcttga taagaaatgg aaggatattg
     1441 tttttcttta tttctagaaa caattaaata aggaattgct aatttttgaa gtacgtattt
     1501 tactgctttt gctgctcctc cattgccaag tattaaggct ttttgatgct gaggttgaag
     1561 cagtgatgta aaagattttt cgaaaccgat tatgtctgta ttaaaaccat ataatacatt
     1621 atctgtaatt tttatacaat tacaagcact aattgtttct acctcttttg tagcaaaatt
     1681 taagaaaggt aaaacagctt gtttataagg tattgtaact gctaacccac ataagttttt
     1741 agtagtagca agcaattctt ttaattgatt aatatcagta agctcaaaat ttttaaaata
     1801 ataatcgggt aaattctctt ttagaaattt atcttcaaag tatttttttg acatagagtg
     1861 agaaagagaa tttccaacta agccaaatat tttcattaat aaattgcttt ttctttatct
     1921 aaatataatc taaaagtatc gccacgtaaa ccaactctca tagcttctaa tgctaatatt
     1981 tctgttggtg caatatttcc taaattaaca ttacaaccaa ttagttctaa aaagtatagt
     2041 tgctgtgctt tttgtggagc ttcccaaata attttctcac ttggtatttg agttaatatt
     2101 tcttgtacta atccttctct tacttctcct gtacctctat aaatacctac attacctgct
     2161 tctcttgctt cggcaattac ataactgcta ccagcttcta attctgctct cattaattca
     2221 atccatttat atggcggaat aatatgtgtt gcatctttgc tacctacttc actaagtacg
     2281 gtagcatatt tagtcatttt ttctatataa ccacattttt ctgtatgggg tattgtaatg
     2341 gagccatcac taacttcaat ataatctatt tcataatctt tacaaattgc taaataatca
     2401 tctatctggt ttcgtattaa aaatgcttca aacaaagtac cgccaaaata aacatgaata
     2461 ccagcagcct tataggtttc tatttttttt cttaggttag gagtaacgtg tgaggttcca
     2521 aaacctagtt taactaaatc tacatgagga gttgctatct ctaaaaaatt ttttgcatct
     2581 gttaacccta atcctttatc cataaccatt gtaagtccat tagttcttgg cttacttgta
     2641 cgatcaggca tttgtgttaa attaaaattc attagttttt attttttata acttttgcaa
     2701 aagtaagctt atgcgtaaaa ctaatttgta gaaattatag gaataaaatg gatgagtgca
     2761 aaattgatat tttttaaaaa aattatttat tgtacttttt atattttgca acaatatcaa
     2821 ccacagcagt attttgaaga attgctggat ttaattctat aaactttttc aataatttag
     2881 gagccttttc cattgctttt tctaattgta ataaaccttc tttagtttta ccaactttta
     2941 aaagtgctgt gctgtaataa aaaataaata tgggtttgtt ttcggtaatt tttaatgcag
     3001 ctttgcattg ttctattgct tcatcataaa atgttgcttt aaataagcag cgtattagag
     3061 cttcccatcc tgctattttt tttggtttgt tatttactac aatgctaaag taaaccactg
     3121 catctttaaa caagtttaac ttcattttac actctcccat agctaagtta tactctggca
     3181 catttttatg tatttgcaat gcttgctcaa gctgcttaat agcttgttgc cattggtttt
     3241 ctgccatgta agtggtagct attttatagt ataatttact atcgtcgggg tttaagtgta
     3301 ctgctttttt gtaatgaaac cttgcctggc cagtattacc cattctatga tacgaatggc
     3361 caattgcttc ataaataaca tcttctggtc tggttaattc taaaactttt tctaatacct
     3421 caatggcttc tctatatttt tttaatcgca aatatgcatc tcccatattt ctatatgcat
     3481 aatcaaactt ttcatcaatt actatt
//
LOCUS       DAOQHP010000237         3500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17089_length_3500_cov_3.889695, whole genome shotgun sequence.
ACCESSION   DAOQHP010000237 DAOQHP010000000
VERSION     DAOQHP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17089_length_3500_cov_3.889695"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLY81_07810"
     CDS             complement(<1..849)
                     /locus_tag="PLY81_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30168.1"
                     /translation="MKLKLNIKTARNLAIASLAVISFSSCKKNNIAVDVDALNTPDAP
                     ALAINAASRTAMYYVSPTSAPYKIPVTVMNVSTGDRAFNIAYTSNTATAGVQYSAPST
                     VTVKAGSVGDSLAVQGIFAGLTAAKDTVKIKFSGTTGLYAKDSFMLVLLKSCPVVLTN
                     MSGAFPGTEEYNSAGVKQWGPYTVDVLNLVAGGSPNTATGQFDGLYDFDWGPINFTMD
                     YSNINPGLWTVTIPLQTTGSTGSGVTHVRGSAGKTNTFSACDQTFTITLDLMTDATTV
                     GAAAYQF"
     gene            complement(892..2382)
                     /locus_tag="PLY81_07815"
     CDS             complement(892..2382)
                     /locus_tag="PLY81_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF30169.1"
                     /translation="MKNKSLSLLLVAAIATTVGCKKGYLDTAPSNAISLEQAYATIAA
                     INSGLTATYNSTFDFAVGNGNTGHDNYGQKSCDLANDLMGQDMIVHSQGYGWYNRDYQ
                     YVEWTLPNGTGTRSDKVWSFYYNVIRQVNLLLDNTKNQAVTPDLESARGQAYGIRAWA
                     YFNLINNFQQTYKGNETAKGVPLFIEPNATSKPRGTVQQVYDQIIADLTQAETLLNGK
                     PRASKMNMDVSVIRGLRARVALVQEDWTAAANYANLAKQPYTLMSNAVYTSRTGFSTI
                     GTSEWMWGTLITAAQATIYASFFSHMDPSQLSYAQLGGQKKIPKALYDLIPASDARKQ
                     VFTAPGTGTANFPDYCQKKHLVPTIGSWAADYLYMRASEMYLIEAEALARKGGAEAQA
                     QAVLQAMVGLRNPTYNAAAFTGTALINEILLQRRIELWGEGFSLFDIKRTHSGLNRAT
                     GAGNHGAPNFNPIVYTQPDASPLFLMRIPQRELDNNTAMTAADQNP"
     gene            complement(2403..>3500)
                     /locus_tag="PLY81_07820"
     CDS             complement(2403..>3500)
                     /locus_tag="PLY81_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30170.1"
                     /translation="ESGNENIGYFYQYKDWYYADGLGNFAPPTRLANKDLKWEKNRAL
                     NYGVEFTMFKRRLQGSIELYDRRSSDLIFGRPIEPSVGAGNDVLENVGQVKNTGIEIQ
                     LGYNVVRKKNFDWRVDFNISSFKNVITELPKAQREKGIISGTKKMMEGKSVYDFWLRE
                     FAGVSAATGRALYYRDVLDNNGKPTGNRVITDDATLASFYYVGGSAIPKFMGGITNSF
                     RYKNFDFSFLMTYSYGGRFYDGNYASIMHYGSAGTALHVDAKNRWQKPGDITNVPRLQ
                     NASGQETSSSRFLLDASYLSIKNVTLSYSLSNNVAKKIHLNGASFFVNVDNAYIFTAK
                     KGSDPQQSFDGVTGAGYPPFRTFTLGATIKL"
BASE COUNT         1123 a          647 c          543 g         1187 t
ORIGIN      
        1 aaattgataa gctgctgcac ctacagttgt agcatctgtc attaaatcta aagttatggt
       61 aaaagtttga tcgcatgctg agaaagtgtt tgttttaccg gcagaacctc taacatgtgt
      121 tactccacta cctgtggagc ctgttgtttg taatggtata gtaactgtcc ataatccagg
      181 atttatatta ctatagtcca tagtaaagtt aattgggccc caatcaaagt catataaacc
      241 atcaaattgt cctgtagcag tgttaggaga acctcctgca actaaattta aaacgtctac
      301 agtatatggg ccccattgtt ttacaccagc actattgtat tcttctgtac caggaaaagc
      361 accagacata ttagttaata ctacagggca agattttaac aatacaagca taaaagaatc
      421 tttagcatac aaacctgttg taccactaaa tttaatttta acagtatctt ttgctgcagt
      481 taaaccagca aaaatacctt gtactgctaa agaatctcct actgagccag ctttaactgt
      541 tacagtagaa ggtgcactat actgaacacc agcagttgca gtgtttgaag tgtatgcaat
      601 attaaatgct ctatctccag tacttacatt cattactgta actggtattt tgtatggtgc
      661 agatgttgga gaaacataat acattgctgt tctagatgca gcattaatag ccaatgcagg
      721 ggcatcaggc gtatttaatg catctacatc tactgcaata ttatttttct tacaagaact
      781 aaagctaata acagctaatg atgctattgc caaattcctt gcagttttaa tatttaattt
      841 aagtttcatt ctttaaagtt ttaatgtttt atttatttaa tacaaaataa attaaggatt
      901 ttggtcagct gctgtcattg cagtattatt atccaactct ctttgaggaa ttctcattaa
      961 gaacaatgga ctagcatctg gctgtgtata tacaattgga ttaaagttag gagcaccatg
     1021 gttacctgca ccagttgcac ggtttaaacc agagtgtgtt ctcttgatat caaataaaga
     1081 gaaaccttca ccccataatt caattcttct ttgtaataaa atttcattaa ttaaagcagt
     1141 acctgtaaag gctgcagcat tataagttgg gtttcttaag ccaaccattg cttgtaatac
     1201 agcttgtgct tgagcttcag caccaccttt tctagctaat gcttctgctt caattaaata
     1261 catttcagaa gctctcatgt acaagtaatc agctgcccaa ctaccaattg ttggtactaa
     1321 gtgttttttc tgacaataat cagggaaatt tgcagtacca gtacctggtg cagtaaatac
     1381 ttgtttacga gcatcagaag caggaattaa atcgtataat gcttttggta tttttttctg
     1441 acctcctaat tgtgcatagc ttaattgaga agggtccatg tgagagaaga aagaagcgta
     1501 aattgttgct tgtgcagcgg taattaaagt accccacatc cactcacttg taccaattgt
     1561 tgaaaatcct gttcttgaag tatatacagc attagacatt aaagtataag gctgcttagc
     1621 taaattagca tagttagctg cagcagtcca atcttcttgc actaaagcaa ctcttgcacg
     1681 taaacctctt ataacactta catccatatt cattttagat gctctaggct taccgtttaa
     1741 taaagtttcg gcttgtgtta aatctgcaat gatttgatca taaacttgtt gaactgtacc
     1801 tcttggtttt gaagttgcat ttggttcaat aaataaagga actccttttg cagtttcatt
     1861 tcctttatag gtttgttgaa aattattaat caagttaaaa taagcccaag ctctaatacc
     1921 ataagcttgt cctcttgcac tttctaaatc tggagttaca gcttgatttt tagtgttgtc
     1981 taataataag tttacttgtc taattacatt ataataaaaa gaccaaactt tatcagaacg
     2041 tgtacctgta ccatttggta aagtccactc tacatactga taatctctgt tataccagcc
     2101 ataaccttga gagtgaacaa tcatatcttg tcccattaag tcattagcta aatcgcaaga
     2161 tttttgacca tagttatcat gaccagtatt tccattacct actgcaaaat caaaagttga
     2221 attgtaagtt gcagttaagc cagagttgat agctgctatt gtagcatatg cctgctccaa
     2281 tgaaatggca ttagaaggag cagtatctaa ataacctttc ttacaaccaa ctgttgtagc
     2341 tattgcagca acaagtagta agcttaaaga tttatttttc atattcaaat ttttaaaatt
     2401 ttttataatt taattgttgc acctaatgta aatgttctga atggaggata tcctgcacca
     2461 gtaactccat caaaactttg ttgagggtcg ctaccttttt tagcagtgaa aatgtaagca
     2521 ttgtccacat ttacaaagaa agaagcacca ttcaaatgaa tttttttagc aacattattt
     2581 gataaactat aacttaatgt tacgttttta atacttaaat aagaagcatc taataaaaat
     2641 cttgatgaag atgtttcttg accagaagca ttttgtaaac gaggtacatt tgttatatct
     2701 ccaggttttt gccaacgatt tttagcatcc acatgtaatg cagtacctgc acttccgtaa
     2761 tgcataatgc tagcatagtt tccatcgtaa aaacgaccac cataagaata agtcattaag
     2821 aaagagaagt cgaagttttt ataacggaat gaattggtaa taccacccat aaatttagga
     2881 atagcagaac caccaacata gtagaatgaa gctaaagttg catcatcagt tattactctg
     2941 tttcctgttg gtttaccatt attgtctaat acatctctat agtataatgc tctaccagtt
     3001 gctgcactaa caccagcaaa ttctctcaac caaaaatcat atactgattt tccttccatc
     3061 attttctttg ttccagaaat tatacctttt tcacgttgtg cttttggtaa ttctgtaatt
     3121 acgtttttga aagaagagat attaaaatct actctccaat caaagttttt ctttctaaca
     3181 acattataac ctaattgaat ttcaatacca gtatttttaa cctgaccaac attttctaat
     3241 acatcatttc cagcaccaac tgatggttca attggacggc caaaaattaa gtcagatgaa
     3301 cgtctgtcat ataattcaat agatccttgt aatcttcttt taaacatagt gaattcaaca
     3361 ccatagttta aagcacggtt tttctcccat ttcaaatctt tatttgctaa tcttgtagga
     3421 ggagcaaagt ttcctaatcc atcagcataa taccaatctt tatattgata gaaataacca
     3481 atgttttcat taccagactc
//
LOCUS       DAOQHP010000238         3500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17091_length_3500_cov_3.734107, whole genome shotgun sequence.
ACCESSION   DAOQHP010000238 DAOQHP010000000
VERSION     DAOQHP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17091_length_3500_cov_3.734107"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1561)
                     /locus_tag="PLY81_07825"
     CDS             complement(143..1561)
                     /locus_tag="PLY81_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF30171.1"
                     /translation="MFFQHVFDKSLAQSSYFIGCQATGEAIVIDAKRDIDTYLEIAQQ
                     NNLKITHITETHIHADFLCGSRELEAVTGARIYLSDEGGKDWQYEFKHIGLKDGDTIQ
                     VGNLTLNVLHTPGHTPESISFVLTDNPASNKPVMVFTGDFVFVGDIGRPDLLEKAAGI
                     IGTKEIGAKQMYQSLQKFTSLPDYVQVWSAHGAGSACGKALGAVHCSTVGYEKIRNWA
                     FQYNNKEQDFIDYLLADQPEPPKYFAMMKHLNKVNRRLLVVVPQQIKLTNSQFLSAYK
                     KGLKIIDTRNKQEFAKAHLPGSINIQNNNALSTWAGWFLNYQEQFILIADDNCIEEIT
                     RKLMRIGLDNVYGYISNINDLNVELHQVNNIQLSEFSELIKKDNVQIVDVRALTEYNA
                     AHIEKADHVFLGTIQNNLNKISRDKEVIIHCQAGDRSSTAYSILKSNGFHNIKNFSGG
                     MKSWLESKKNTVSYSNHVCKLN"
     gene            complement(1705..2328)
                     /locus_tag="PLY81_07830"
     CDS             complement(1705..2328)
                     /locus_tag="PLY81_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF30172.1"
                     /translation="MEELGQIEEFKSSPELVEKLYQHGIIKNYEAGSIILNEHSHIRS
                     IPIVTSGIIKVIRTDEEGREILLYYIKAGESCIMSFLGGMHNEASIVKAEVEEDAEIL
                     FLPIEKVSLFIKEYPQWLEYIFKLYHKRFEELLEIINAIAFKKVDERLLTLLHKLANL
                     TQSNIIAITHEQLANKLGTVRVVVSRLLKQLEEEGKVQLGRNKITLL"
     gene            complement(2355..2702)
                     /locus_tag="PLY81_07835"
     CDS             complement(2355..2702)
                     /locus_tag="PLY81_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30173.1"
                     /translation="MKLEHKQQNIIPFPKFLLRLSRYAIFAFSLILLSVLIGTLGYHH
                     FGKIGWLDSFHMACMILTGMGPVIEMQSVSAKIFSSLYALYSGIAFLSLTAVFFTPII
                     HRLLHILHIDQNK"
     gene            complement(2713..>3500)
                     /locus_tag="PLY81_07840"
     CDS             complement(2713..>3500)
                     /locus_tag="PLY81_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30174.1"
                     /translation="LLTNKFSFAALNTANAIGVIDNLTMYARLKKDERTTPKLERRVV
                     MLELLQKITQRINIASLEISAVASELDCEEERASQVATYLKSKESAKEKNLIIGSMIV
                     GAVGSIAAEIISMNSNSGNTSNIVAITTSIIDVGLGTLIILNNKSIEFYHNRNALKDI
                     WNNPKTSSFFTPSIWYYLTYENVSKNEKSLRKQLIEKWQDFGQITSSKNKDKNIELYF
                     GNGGKYETEQLKNRADMHDQIESYITLMKQDLKQLSIEIENLN"
BASE COUNT         1116 a          586 c          480 g         1318 t
ORIGIN      
        1 tgtttattgc acctttaaca ctgccagtcg aaaactctgc aggtgtacga acatcaacca
       61 ataaagcacc ttgtttacaa aggtctgcaa ttttattttt atttgcagag ccaaaaagca
      121 ttgaaaaaat tcccatatta tattaattta atttacaaac atgattacta taactaacgg
      181 tatttttttt actctctaac caacttttca taccgcccga aaagtttttt atattgtgaa
      241 agccattact ttttaatata gaataagcag tagaagatct atcgcctgcc tggcaatgaa
      301 taattacttc tttatctctg cttatcttgt ttaaattatt ttgaattgtg cccaaaaaaa
      361 catgatctgc tttttcaata tgtgctgcat tatattctgt taatgctcta acatctacaa
      421 tttgtacatt atccttttta atcaattccg aaaattcgct aagctgtatg ttattaactt
      481 gatgcagctc aacatttaaa tcgtttatat tgcttatata gccatataca ttatctaaac
      541 caattctcat taattttctg gtaatttcct ctatacaatt atcatctgct attaaaataa
      601 actgttcttg ataatttaaa aaccaaccag cccaagtaga aagggcattg ttgttttgaa
      661 tgttaatact accaggaagg tgagcttttg caaactcttg tttatttctt gtatcaataa
      721 tttttaatcc ctttttatat gcagaaagaa actgactgtt ggttaatttt atttgttgag
      781 gcactaccac caaaagccta cgatttactt tattcaaatg cttcatcata gcaaaatatt
      841 ttggaggctc tggttggtcg gctaataaat aatcaataaa atcttgctct ttattattgt
      901 attgaaaagc ccagttgcga attttttcgt aaccaacagt agagcaatgt actgcaccta
      961 atgctttgcc acaggcagag cctgcaccat gagcagacca aacttgcaca taatcgggca
     1021 gagaagtaaa tttttgtagt gattgataca tttgcttggc tcctatttct ttagtgccta
     1081 ttatgcctgc tgccttttct aataaatctg gtcttccaat atcgccaaca aaaacaaaat
     1141 ctcctgtaaa aaccattaca ggtttattgc ttgcaggatt atcggttaaa acaaatgaaa
     1201 tactttctgg tgtatggcct ggtgtgtgta acacatttaa agttaaatta cctacttgaa
     1261 tagtatcgcc atcttttaag ccaatatgtt taaattcata ttgccagtct ttaccgccct
     1321 cgtcagataa atatattctt gcaccagtaa ctgcttctaa ttctcttgag ccacaaagaa
     1381 aatcggcatg aatatgtgtt tctgttatat gtgttatttt taaattattt tgttgggcaa
     1441 tttctaaata ggtatcaata tctctttttg catctattac aattgcttct cctgtagctt
     1501 ggcagccaat aaagtagcta ctttgtgcaa ggcttttatc aaatacatgt tgaaaaaaca
     1561 tagcgaaata ttttaagaca ccaaagatga atatatttaa aaatatagtt ggttactttt
     1621 gttacactat gcaatgtttt acatttcttt aataaaatga atctttaaat aaataagttt
     1681 taaggaataa aaaacaagca gcaactataa taaagtaatt ttattgcggc ctaattgcac
     1741 ttttccttct tcttctaatt gctttaataa tcttgaaaca acaacacgaa cagtacctaa
     1801 tttatttgct agttgttcgt gagttatggc tataatatta gattgagtaa gatttgctaa
     1861 tttgtggagc agggttaaca acctttcatc taccttttta aaagctattg catttataat
     1921 ttctaataat tcttcaaaac gtttgtgata caatttaaaa atatactcta accattgagg
     1981 atattcttta ataaacaacg aaactttttc aataggtaaa aaaagtattt cagcatcttc
     2041 ttctacttct gcttttacta tacttgcctc attgtgcatg ccacctaaaa aagacatgat
     2101 acaactttcg ccagctttta tataatacaa taaaatttct cttccttctt catctgttct
     2161 aatcactttt ataattccag atgttacaat aggtatagac ctaatgtgtg aatgttcatt
     2221 taatattata ctacctgctt cataattttt tataatacca tgttggtata atttttcaac
     2281 caattctgga gacgatttga attcttctat ttgccctaat tcttccattg taaatatttt
     2341 atgtgctata aagtttattt attttgatct atatgaagta tatgcaaaag cctatgaatt
     2401 attggtgtaa agaaaacagc agttaagctt aaaaatgcaa taccactata taatgcatat
     2461 aatgatgaaa aaattttagc agataccgat tgcatttcta ttactggccc catgcctgtt
     2521 aatatcatac aagccatgtg aaaactatct aaccaaccaa tttttccgaa atgatgatag
     2581 ccaagtgtgc caattaaaac agataacaat attagtgaaa aagcaaaaat tgcatatcgg
     2641 ctcaatcgta ataaaaactt aggaaaagga ataatatttt gttgtttgtg ttcaagtttc
     2701 atttttaaaa tattaattta agttttcaat ttctattgaa agttgtttta aatcttgttt
     2761 cattagggtt atataagatt caatttggtc atgcatatct gcacggtttt ttaattgttc
     2821 cgtctcatat tttcctccat taccaaagta aagttctata tttttatctt tatttttgga
     2881 tgaagttatt tggccaaaat cttgccattt ttctatcaac tgtttacgta aagatttttc
     2941 gtttttagat acattttcat aagttaaata ataccaaatg gaaggtgtaa aaaaagaaga
     3001 tgtttttgga ttgttccaaa tgtcttttaa tgcatttcta ttatgataaa attctatgct
     3061 tttattgttt aaaattataa gtgtgcctaa gccaacatct attatactgg ttgtaattgc
     3121 aacaatattt gaggtgttac cactatttga attcattgaa attatttctg cagcaataga
     3181 gcctactgct ccaacaatca tagagccaat gattaaattt ttttctttgg cactttcttt
     3241 acttttcaaa taggtggcta cttggcttgc cctttcttct tcacaatcta attcgcttgc
     3301 cactgccgaa atttctaaag aggctatatt tatacgttgt gtaatttttt gcaacaattc
     3361 taacataacc actcttcttt ctaattttgg tgttgttctt tcgtcttttt ttaacctggc
     3421 atacatagtt aaattatcaa taacacctat ggcgttggct gtatttaagg cggcaaaaga
     3481 aaacttattg gtaagcaagc
//
LOCUS       DAOQHP010000239         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17151_length_3493_cov_3.773997, whole genome shotgun sequence.
ACCESSION   DAOQHP010000239 DAOQHP010000000
VERSION     DAOQHP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17151_length_3493_cov_3.773997"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLY81_07845"
     CDS             complement(<1..397)
                     /locus_tag="PLY81_07845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30175.1"
                     /translation="MMETEREQHWNNVYATKLPNQVSWTQAIPTIALNYFQLLQLNKQ
                     ASIIDIGGGESTLVDFLVEQGFENITVLDISTIAIQKVKQRLGDKAKKINWIVTDILN
                     FKPTQKYDVWHDRATFHFLTNHEHINKYVS"
     gene            complement(424..2640)
                     /locus_tag="PLY81_07850"
     CDS             complement(424..2640)
                     /locus_tag="PLY81_07850"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNF30176.1"
                     /translation="MKVLLSFLILFFSITIHAQQRLVDFVNPFIGTGGHGHTYPGATI
                     PFAMVQLSPDNGTNGWDWVSGYHYSDSVIQGFSHTHLSGTGVGDLCDISVLPIVNRIA
                     SAEKITSKFSHKQETAKPGYYAVHLKDFDVWAEHTTTIRCGFHRYTFPASKNAAIRFD
                     LGFAINYDRTTECSFKKINDTTFVGYRFSKGWAPNQKIFFAVRVSKPVKNIVIIANKE
                     KIDTNNYTAKDIKAWLEFETNLGEKILMKVGLSSADIEGALESLNEINGWNFDLVKQN
                     AENIWERELRKLQIRTNDIELKQNFYTALYHTFLAPNRFSDRYGNYKSANGNIEKGKS
                     IFTVQSLWDTFRAANPLLTITQTELVPAIINSYLTFYDQYGLLPVWELQFNETNCMTG
                     YHAVPIIADAILKGITGFDYNKAYEAMKVSANQNIRAADFYRHFGFVPADKAGQSVTI
                     TLEYAFDDWCIAQVAKRLGKSADFREFRRRSMNWRNVFDKKTGFARAKLSNGNWVTPF
                     DPFHAEHDPEKTPYTEGNAWQHTWFAPHDVYALINYMGGAKKFEIRLDSLFKADSKLT
                     GSHVPPDISGMIGQYVHGNEPSHHIAYLYNYIGKPYKTVDKVKEICTTLYHNKPDGLP
                     GNEDCGQMSAWYVFSVLGFYPVNPASGEYVLSVPLVQESALQLPDGHLFRVIVKEANE
                     KNKYIKQATLNGNPYTKSFLKHSDIMKGGILEITLADKPGTSFGTAMEDLPGRTAK"
     gene            complement(2761..3183)
                     /locus_tag="PLY81_07855"
     CDS             complement(2761..3183)
                     /locus_tag="PLY81_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF30177.1"
                     /translation="MIKLQVIGNLGKDATVNNVNGKNVINFNMAHTERYKDAQGNQKD
                     RTIWVECAYWTDKTAIQPYLKKGTQVYVEGNPDVRTYQTQDGKNGASLTLRIQNIQLL
                     GTRSSDNTSAVQNNTPPQQNTNFTPTSDMNSVADDLPF"
     gene            complement(3313..>3493)
                     /locus_tag="PLY81_07860"
     CDS             complement(3313..>3493)
                     /locus_tag="PLY81_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNF30178.1"
                     /translation="NQVGSTSLIQRRMKLGYNRAGRLMDQLERAGIVGPNLGSKAREV
                     KFKSETELDDFLQSI"
BASE COUNT         1044 a          600 c          549 g         1300 t
ORIGIN      
        1 gcgatacata tttgttaata tgttcatgat tagtaagaaa gtgaaaagta gctctatcgt
       61 gccacacatc gtatttttga gtaggtttaa aatttagtat atctgttact atccaattta
      121 ttttttttgc tttatcgcct aacctttgtt ttactttttg aattgcgata gttgaaatat
      181 ctaaaacagt tatgttttca aacccttgtt ctactaaaaa atcaaccaag gtgctttctc
      241 cacctcctat atctattatg cttgcttgtt tatttaattg taataattga aaataattta
      301 atgcaatagt tggaatggct tgtgtccaac ttacttgatt tggcaatttt gttgcatata
      361 cattattcca atgttgttct ctttctgttt ccatcataac atcatactac ataatacggt
      421 ttattatttt gcggttctgc caggaaggtc ttccatagca gttccaaagc ttgtacctgg
      481 tttatctgct aaagtaattt ctaaaattcc tcctttcatt atatctgaat gttttaaaaa
      541 ggatttggtg taaggattac cattaagagt agcctgtttt atgtatttgt ttttttcatt
      601 tgcctctttt acaattactc taaataaatg tccatcgggc aattgtaatg cactttcttg
      661 tactaatgga acacttaaaa catattcgcc acttgcaggg tttacagggt aaaatcctaa
      721 aacactaaat acataccaag cactcatttg cccacaatct tcattacctg gtaagccatc
      781 tggtttgtta tggtaaagag tggtacaaat ttcttttact ttatctactg ttttatatgg
      841 tttaccaata tagttataca agtaagcaat atggtggctg ggttcgttgc catgtacata
      901 ttgcccaatc ataccactta tatctggtgg tacatgtgag cctgtaagtt tagaatcggc
      961 tttgaataaa gagtctaatc ttatttcaaa ttttttagct ccacccatat aattgattag
     1021 tgcataaaca tcgtgtggtg caaaccaggt atgttgccag gcatttcctt cggtgtaagg
     1081 tgttttttct ggatcatgtt ctgcatgaaa tggatcaaaa ggggtaaccc aattgccatt
     1141 acttaatttt gcacgggcaa agcctgtttt tttatcaaat acatttctcc aattcatact
     1201 acgtcttcta aattctctaa aatcagccga ttttcctaaa cgtttagcta cttgtgcaat
     1261 acaccaatca tcaaaagcat attctaaagt aatagttaca ctttgccctg ctttatctgc
     1321 tggaacaaag ccaaaatgcc tataaaaatc tgcagctcta atattttggt tggcacttac
     1381 tttcattgct tcataagctt tgttataatc aaagcctgta atacctttta aaattgcatc
     1441 ggcaataatg ggcacagcat ggtagcctgt catacaattg gtttcgttaa attgtaattc
     1501 ccatacaggc agcaagccat attgatcgta aaaagttaaa taagaattga tgattgccgg
     1561 aactaattct gtttgtgtaa ttgttagtaa tgggttagct gctctaaatg tatcccataa
     1621 agattgaaca gtaaagattg attttccttt ttctatattt ccattggcac ttttataatt
     1681 accatatcta tcactaaatc tattgggtgc taaaaaagta tggtataaag cggtatagaa
     1741 attttgtttt agttctatat cgttggtacg aatttgtaat tttcttaatt ctctttccca
     1801 aatattttcg gcattttgtt taactaaatc aaagttccaa ccgtttattt catttaagct
     1861 ttctaaagca ccttcaatat ctgcactgct taaacctact ttcattaaaa tcttttcgcc
     1921 caaatttgtt tcaaactcta accatgcttt aatgtctttt gcagtataat tgtttgtgtc
     1981 tattttttct ttgttggcaa taattacaat attttttacg ggcttactta ctcttactgc
     2041 aaaaaatatt ttttgattgg gtgcccagcc tttgctaaaa cggtagccta caaaagtggt
     2101 atcattaatt tttttaaaac tacattctgt agtacggtcg tagttaatag caaaacctaa
     2161 atcgaaacga atggctgcgt ttttggatgc aggaaaagta taacgatgaa agccacaacg
     2221 aatggttgtt gtatgctcgg cccatacgtc aaaatctttt aaatgtacag cataatatcc
     2281 tggtttggca gtttcttgct tatgagagaa tttgctagtt attttttctg ccgatgcaat
     2341 tctatttaca ataggcaaaa cagaaatatc acataaatcg ccaacacctg taccacttaa
     2401 atgggtatga ctaaaccctt gtattacact atcgctataa tggtaaccac tcacccaatc
     2461 ccagccattg gtgccattat ctgggcttaa ttgaaccatt gcaaaaggta ttgttgcacc
     2521 agggtaagta tgcccatgac cgcctgtgcc aataaaggga ttcacaaaat ctactaaccg
     2581 ttgttgagca tggatagtta tggagaaaaa taaaattaaa aaagatagta aaactttcat
     2641 gaatttattt attctttata atttattaat taaaaaatca atttagtaga agcagttgtt
     2701 caaaaaattg gttgatgttt ttcatcaacc aattgtatat tttaaaataa aaaagtttac
     2761 ttaaaatggt aaatcatcag caactgaatt catatctgat gttggtgtaa aatttgtgtt
     2821 ttgttgtggt ggcgtattat tttgaacggc tgaagtattg tctgaacttc ttgtgcctaa
     2881 taactgaata ttttgaattc ttaaagttaa agaagcacca ttttttccat cttgggtttg
     2941 gtaagttcta acatcagggt ttccttctac ataaacttgt gtgccttttt ttaaataagg
     3001 ttgaatagca gttttatctg tccagtaagc acattctacc caaatggttc tatctttttg
     3061 gttcccttgt gcatctttat acctttcggt atgtgccatg ttaaagttaa ttacattttt
     3121 gccatttaca ttgtttacgg tagcatcttt tccaagattg ccaattactt gtagcttaat
     3181 cataacagtt cgttttggta aattgagtta atataaaatt taagaactgc caagatagtt
     3241 aattaaggta taaaccaaga aagatttata ctattttatc cacttgagaa aaatgattgc
     3301 agtaaattta ttttatatag attgtaagaa atcgtctaat tctgtttccg acttaaattt
     3361 aacttctctt gctttgctac ctaaattagg cccaacaata cctgcacgtt ctaattggtc
     3421 cattaatctt cctgcacggt tatatcctaa tttcattctt cgttgtatga gagaggttga
     3481 gccaacctga ttt
//
LOCUS       DAOQHP010000240         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17242_length_3482_cov_2.866939, whole genome shotgun sequence.
ACCESSION   DAOQHP010000240 DAOQHP010000000
VERSION     DAOQHP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17242_length_3482_cov_2.866939"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLY81_07865"
     CDS             <1..428
                     /locus_tag="PLY81_07865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF30179.1"
                     /translation="KEDLEVYITSKKLANKIKVCDGVFGKEKFKVIEASDIFIFPTYY
                     PQEVFPGVVLEAMQCAKPIITTTTGVIPEIIDDGINGLIAHKKDTALFVELAESLINN
                     KKMAMQMGKAAREKYLSSYTQEIFENNVKDTIINVVELN"
     gene            complement(420..998)
                     /locus_tag="PLY81_07870"
     CDS             complement(420..998)
                     /locus_tag="PLY81_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30180.1"
                     /translation="MFNIKQLIKNILSYPIAYFILLKTRKLIIGSSNKRIFLFDIDNT
                     IADSWHSLTLNIWANENERLKGLAIFLRMRKLVTILQKNKNNQVIFLTARSYFSRQTT
                     ENWLHENGILQTSKNLIITRTAKDKINIIKSLPLAKLKIFYIDDLSHKHEKGTVEMFE
                     DEINCIKKLEKLNTKNFTYYGKSEIDKFCSLV"
     gene            1107..2402
                     /locus_tag="PLY81_07875"
     CDS             1107..2402
                     /locus_tag="PLY81_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30181.1"
                     /translation="MELAGINSRNIANGLYIINQYPKYLQSQYFSAEKIKLIQLKKLE
                     AIVRVAINTVPYYKALNVKINFNNFSQQELLKFPIITKAIIRDNYNSFISEKYFNKKL
                     QYKSTSGSSGQPFKVPKPFYSDGIEQCMMFRAWSMNPAFKYSFHKPCIVLRSFSPKQG
                     EPPYKVDKILNYWYLSPFDINQQNLETYVQFIKKSKAQLLRGYPSSIYILTLLLKENN
                     IKLPQIKTLFTSSENLLPNYRKVIEDYWQMPLIDWYGQNERTVTVQMCEHGNYHNNDE
                     YGIVELDENNQIIATSLNNNIMPLIRYATNDIAIPAINPSCNCGRGLSIPFSGIDGRA
                     DDLLIKEDNTIIATVNIYNAMEKFPDVKQFKIIQQKDKSLELYLSENTKLSPEYICNI
                     EKELKQRLGNVRIITKVVEEIERNKNTEKIKSIESHIKL"
     gene            2406..>3482
                     /locus_tag="PLY81_07880"
     CDS             2406..>3482
                     /locus_tag="PLY81_07880"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNF30182.1"
                     /translation="MPIKGLTNEQAAMVQHIKLLKQQAGSHSPSLFTIKEQLPDLKMN
                     IDACFLSNPYATDLFLRYFKNELLGDDLLIRDMLEFYPSQNNVIASSLANFLKVPADM
                     LFIGNGAIEIIQAVIHNFTTEKIIVNIPTFSSYYEFVQPGVSIVFNKLKKENDYKLDV
                     NDLLQLVNYEKPDTVVLINPNNPNGGYITKAEIAELLEGLKNVRTVLLDESFIHFAFE
                     NEAMELMSYADMVMKYPNVVTIKSMSKDFGIAGIRAGYAIMHPSRAKQLLENGFLWNS
                     NGLAEYFFKLYVRDQFQKEYEVERKRYIRDTQEYFKNLSAIEGIKRYPSQANFALIEL
                     NTGITADDFAFGLLVKSGVYTRTCS"
BASE COUNT         1315 a          455 c          527 g         1185 t
ORIGIN      
        1 ctaaagaaga cctggaagtt tatattacaa gcaaaaaatt agcaaataaa attaaagttt
       61 gtgatggggt gtttggtaaa gaaaaattta aagtaataga agcatctgat atttttattt
      121 ttccaactta ttaccctcaa gaggtttttc ctggagttgt tttagaagct atgcaatgtg
      181 ctaagccaat tataacaact acaacaggtg taatacctga aattattgat gatggaataa
      241 acggtttaat tgcacataaa aaggatactg ctttatttgt agaattggca gaaagtttaa
      301 tcaataataa aaaaatggct atgcaaatgg gtaaggctgc aagagaaaaa tatttaagca
      361 gctacactca agaaattttt gaaaacaacg taaaggatac aattattaat gttgtagagt
      421 taaactaaac tacaaaactt gtcaatttca cttttgccat agtaagtaaa atttttagta
      481 tttaatttct ctaatttttt aatacagtta atttcatctt caaacatttc cactgttcct
      541 ttttcatgtt tgtgagataa gtcatctata taaaaaattt tcaattttgc taatggtaaa
      601 ctttttataa tattaatttt atcttttgca gttctggtaa taattaaatt tttagaggtt
      661 tgtaaaatgc cattttcatg taaccagttt tcggttgttt gcctcgaaaa ataactacgt
      721 gcagttaaaa aaataacttg attgttttta tttttttgca atattgttac tagttttctc
      781 attcttaaaa aaatggccaa cccttttaac ctttcgtttt cgtttgccca aatgttaagt
      841 gttaaagaat gccaactatc tgcaatggta ttatcaatat caaataaaaa aattctttta
      901 ttgctactac caataattaa ttttcttgtt tttaataata taaaataagc aatagggtag
      961 cttaaaatat tttttattag ctgctttatg ttaaacatat tttgtgtaag taattaccaa
     1021 tacaaatatg cagcaaaata attgttcaat ttatttttct ttctttttat aaaatcacac
     1081 aataaatttt taggtaattt aagtttatgg agctagcagg aataaatagc agaaatattg
     1141 ccaatggttt atacataatt aaccaatatc caaaatatct tcaatcgcaa tatttttctg
     1201 ccgaaaaaat taaattaata cagctaaaaa agttagaagc tattgtaagg gtagcaataa
     1261 atactgttcc ttattataaa gcactaaatg taaaaataaa ttttaacaac ttttctcagc
     1321 aagagttatt aaaatttcca ataattacca aagctataat aagagataat tacaacagtt
     1381 ttatttcaga gaaatatttc aataaaaagc tgcaatataa atctacttca ggctccagtg
     1441 gacagccatt taaagtgcca aaaccttttt atagcgatgg tattgagcaa tgcatgatgt
     1501 ttagggcttg gtctatgaat cctgcattta agtatagctt tcataaacct tgtattgtgt
     1561 taaggagctt ttcgccaaaa caaggtgagc cgccttataa ggtagataaa attttaaatt
     1621 actggtactt atctccattt gatataaatc aacaaaactt agaaacctat gtacagttta
     1681 ttaaaaaatc taaagcacaa ctgctacgtg gttatccaag ctctatttat attcttactt
     1741 tattattaaa agaaaataat attaagttac ctcaaattaa aacccttttt acatcgtccg
     1801 agaatttatt gcctaattat agaaaagtaa ttgaagatta ttggcaaatg cctttaatag
     1861 attggtatgg acaaaatgaa agaactgtta ctgtgcaaat gtgtgaacat ggaaattatc
     1921 ataacaacga tgaatatgga atagtagaat tagatgaaaa taatcaaatt attgctactt
     1981 ctttaaacaa caatattatg cctttaataa ggtatgcaac taacgatatt gccatacctg
     2041 ctattaatcc ttcttgcaat tgtggacgag gacttagcat tccttttagt ggtattgatg
     2101 gcagagcaga tgatttatta attaaagaag ataataccat tattgctact gtaaatattt
     2161 acaatgcaat ggaaaaattt ccagatgtaa aacagtttaa aataattcaa caaaaagata
     2221 aatctttaga attatatctt tcagaaaaca ccaaactttc tccagagtat atttgtaata
     2281 ttgaaaagga gttaaagcaa cgattaggta atgtacgcat tataactaaa gtggtagagg
     2341 aaatagaaag aaataaaaat accgaaaaaa ttaaatcaat tgaatcacat ataaaattat
     2401 aaagtatgcc aattaaagga ttaactaacg aacaagctgc aatggtgcag catataaaat
     2461 tgctaaaaca acaagctggc tcacactcgc ctagtttgtt tactataaaa gagcaattac
     2521 cagatttgaa aatgaatata gatgcatgtt ttctttcaaa cccatacgca acagatttgt
     2581 ttttaagata ttttaaaaat gaattgttgg gagatgattt attgataaga gatatgttgg
     2641 agttttatcc ttctcaaaac aatgtaattg catcttcatt agccaacttc ttaaaagttc
     2701 ctgccgatat gttatttatt ggcaacggag ctatagagat aattcaggca gtaattcaca
     2761 attttactac agagaaaatt attgtaaata ttcctacatt ttcttcctac tacgaatttg
     2821 ttcaaccagg tgtaagtatt gtttttaata aattgaaaaa agaaaatgat tataagttag
     2881 atgtaaacga tttattgcaa ttggttaatt acgaaaagcc cgatacagtt gtgttaatta
     2941 atccaaacaa ccctaatgga gggtatatta ctaaagcaga aattgcagag cttttagaag
     3001 gattgaaaaa tgtaagaaca gtattattag atgaaagctt tatacatttt gcttttgaaa
     3061 atgaagcaat ggaattaatg agttatgcag atatggtaat gaaatatcct aacgtagtaa
     3121 ccattaaaag tatgtcgaaa gattttggaa ttgcaggtat aagagctggt tatgctatta
     3181 tgcatcctag ccgtgcaaaa caacttttag aaaatggttt tttatggaat tcaaatggat
     3241 tggcagaata cttttttaaa ttgtatgtaa gagatcagtt tcaaaaagag tatgaagtag
     3301 aaagaaaaag atatataaga gatacacaag agtattttaa aaacctaagt gcaatagagg
     3361 gtattaaacg atatccaagc caagcaaact ttgcattaat agaattaaat acaggaatta
     3421 cagccgatga ttttgctttt ggtttattag ttaaatctgg cgtttataca cgtacttgtt
     3481 cc
//
LOCUS       DAOQHP010000241         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17302_length_3475_cov_4.513450, whole genome shotgun sequence.
ACCESSION   DAOQHP010000241 DAOQHP010000000
VERSION     DAOQHP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17302_length_3475_cov_4.513450"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1816)
                     /locus_tag="PLY81_07885"
     CDS             complement(293..1816)
                     /locus_tag="PLY81_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF30183.1"
                     /translation="MKKKTALIIGFIGLKFLFHFFLISNEYDLQRDEFLHLDQANHLA
                     WGYLSVPPFTSWTSYIIQLFGNSFFWVKFFPALYGALTIFIVWKSIEELNGNIFALTL
                     GATCVLFSALLRLNILYQPNSLDILCWTAFYFTLLKYIHTENFKWLYIGAVVFALGFL
                     NKYNIIFLLIGLLPALLLTTHRKIFFKPNLYFAALLGFVIISPNLMWQYNNNFPIFHH
                     LKELTETQLVNVDRLDFLKNQLLFFIGSLVVLFSSFYALLFYIPFAKYRVYIYSILFT
                     LTAFVYFKAKDYYAIGIYPIYIAFGSVYLSKILEGGWKKYLQPIAIIIPLLLFIPIYN
                     VAFPNKSPEYIVKHSENYKKLGLLRWEDGKDHLIPQDFADMLGWKELASKVDSIYLSL
                     PNKQNTLILCDNYGQAGAINYYTKQKLQAVSFNADYINWIDLSNKYVNLIRVKEAEEV
                     KEELQKISPYFKNSLLAGSITNPYAREYGTGIFAFIGAKIDIRERLGEEIDKNKNYH"
     gene            complement(1858..2745)
                     /locus_tag="PLY81_07890"
     CDS             complement(1858..2745)
                     /locus_tag="PLY81_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF30184.1"
                     /translation="MKSGIRFVMFFFTLTTLSVQAQRVRLSGALGGSAYYGDLIQGTP
                     LLKQISPAVTLGGSYDLNEKLRVRASLSGLSVKADDKDNSNIKFKNRNLNFKSFIWEF
                     AVAAEYDILNNFDNYAYTPYIFIGPGIFGFNPTTVDRFGNKVNLREWGTEGQGLAAYP
                     NRKPYSRTQFNIGFGGGIRVDLSESLQIGTEIFIRKTFTDYIDDVSINSYVDPSLFVL
                     QGNPYSQYVAFRGDEVGKSFSTTMPRGNPKNKDLFYSFQLKLTYKLLNLDWGGPMGYY
                     GGGSPGRTRDRVRTPRSVL"
     gene            complement(2928..>3475)
                     /locus_tag="PLY81_07895"
     CDS             complement(2928..>3475)
                     /locus_tag="PLY81_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNF30185.1"
                     /translation="KHSTVSSCPFQFNNIIDIGTGSGCIAIEVKNKFKNSTVVAIDKS
                     LDSLEIAKKNALTFNTKINFKHLDFLDEKNWANLGNFDLIISNPPYVLKSEHNTLKEN
                     VLNFEPHLALFVPDNDPLLFFRKIALFGKKHLNKNGKIVVEINELFGYETKQLFIEFG
                     YLSYIIKDMQQKDRFIVSNFS"
BASE COUNT         1265 a          526 c          456 g         1228 t
ORIGIN      
        1 tccaacccaa tcaatttttg agtgttccgt atttacttta taatcatctg ttacaagggt
       61 tttaatacca ggtacaaaag aataaagtgc agtaaaacag gcaatcgcta atgccgaaaa
      121 taaaactttt ttcatgttta attaattgtt taggattcaa atataatggc aaattgtgtg
      181 tatatacatg caaataaatt ctttttcctt attttataaa tttttaacaa tttttagcta
      241 gtaaaaatgg tgcaatttta attactgtta accttgttgt agttgaagta aattaatggt
      301 agtttttatt tttgtcaatt tcttctccaa gtctttctct tatgtcaatt tttgcaccaa
      361 tgaaagcaaa tattcctgta ccatattctc ttgcataagg gtttgttata gagccagcaa
      421 gtaatgaatt tttaaagtag ggcgatattt tttgtaattc ttcttttact tcttctgctt
      481 ctttaactct aattaggttt acatatttgt tggataagtc aatccaatta atatagtctg
      541 cattaaagga aactgcttgt aatttttgct ttgtgtagta atttattgca cctgcctgtc
      601 catagttatc acaaagtatt agtgtatttt gtttatttgg taaggataaa taaatactat
      661 ctaccttact agccaattct ttccagccca gcatatctgc aaaatcttgt ggtattaaat
      721 ggtcttttcc atcttcccaa cgtaataaac ccagtttctt ataattttct gaatgtttta
      781 ctatgtattc ggggctttta tttggaaaag caacattata aataggaata aagagtagta
      841 aaggaataat aattgcaata ggttgtaaat attttttcca accaccttct agaatttttg
      901 aaagataaac agaaccaaat gctatgtaaa ttgggtaaat gccaatggca taataatcct
      961 ttgccttaaa ataaacaaag gctgttaaag taaataaaat tgaataaata tatactctat
     1021 acttagcaaa agggatataa aacaaaagag cataaaatga ggaaaagagt actaccagtg
     1081 agcctataaa aaacagtaat tgatttttca aaaaatcaag tctatctaca tttactaatt
     1141 gtgtttctgt taattctttt aagtgatgaa agattgggaa attgttattg tattgccaca
     1201 taagattagg ggagataata acaaacccta gtaaggctgc aaaatataga ttgggtttaa
     1261 agaaaatttt tctatgtgtt gttagcaaca aagcaggcaa tagaccaatt aataaaaaaa
     1321 taatattgta tttattcaaa aaacctaatg caaaaactac agcacctata tacaaccatt
     1381 taaaattttc tgtatgtatg tattttagaa gtgtaaagta aaaagctgtc caacataata
     1441 tatctaaaga gttaggttga tacaatatgt ttagtcgtaa tagtgctgaa aataacacac
     1501 aagttgcacc taatgtcaaa gcaaaaatat ttccatttaa ttcttctata gatttccata
     1561 caataaaaat tgttaaagca ccatataaag ctgggaagaa tttaacccaa aaaaatgaat
     1621 tgccaaataa ttgaattatg tacgatgtcc aagaagtaaa gggtggaaca gaaagatatc
     1681 cccaagctaa atggtttgct tggtcaagat gcaaaaattc atctcgttgc aagtcatact
     1741 cattgctaat taaaaaaaaa tgaaacaaaa attttagccc aatgaaacca attattaatg
     1801 ccgtcttttt tttcataaag ggctataagt aaaaccaata attacaaaaa ataaatatta
     1861 tagtacagaa cgaggagttc ttactctatc tcttgttctt ccagggcttc caccaccata
     1921 ataacccatt gggccacccc aatctaagtt caataattta taagttaatt ttaattgaaa
     1981 ggagtagaac aaatctttgt ttttagggtt gccacgaggc attgtagtag aaaaagattt
     2041 tcctacttca tctcctctaa acgctacata ctgagaatat gggttgcctt gtaaaacaaa
     2101 taaagaaggg tctacataac tgttaatact tacatcatca atataatcgg taaaagtttt
     2161 acgaataaat atttcggtac caatttgtaa actttcactt aaatcaactc ttataccacc
     2221 accaaaacca atattaaatt gtgtacgaga ataaggtttt ctattaggat aagcagctaa
     2281 accttgccct tctgtacccc attctcttaa atttacttta ttcccaaatc tatctactgt
     2341 tgtagggtta aatccaaaaa taccagggcc aataaaaata tatggtgtat aagcatagtt
     2401 atcaaaattg tttaaaatat catattcggc tgcaactgca aattcccata taaaactctt
     2461 aaagtttaaa tttctatttt taaacttaat attactattg tctttatcat ctgctttcac
     2521 acttaaacca gataagcttg ctctaactct taatttttca tttaaatcat aactacctcc
     2581 aagagttact gcaggtgaaa tttgttttaa taatggcgtt ccttgaatta aatctccata
     2641 ataagcacta ccccctaatg caccagataa tcttactctt tgagcttgca cagataaggt
     2701 agttaaagta aagaaaaaca tcacaaaacg tattccagat ttcatactca taaaatttaa
     2761 cttttcagtt attgccaaaa ttaatgcaaa atattaaaca aaatattttt tatgctacaa
     2821 taataggctt ttcattacaa aatattaatt ttcaataatt ttttttacaa aagtatataa
     2881 aaagggtgtt attttttgtt gaaaaatgct ttataattat tgaattatta agaaaaatta
     2941 cttacaataa acctatcttt ttgttgcata tctttaataa tataggatag ataaccaaac
     3001 tctataaata gctgctttgt ttcataacca aataattcat ttatttctac aacaattttt
     3061 ccatttttat tcaagtgttt ttttccaaac aacgcaattt ttctaaaaaa caataaaggg
     3121 tcattgtcgg gtacaaataa agccaaatgt ggttcaaagt tcagtacatt ttcttttaat
     3181 gtattatgct cactttttaa aacatacggt gggttactaa ttattaaatc aaaatttcct
     3241 aaatttgccc agttcttttc atctaaaaaa tctagatgtt taaaatttat ttttgtatta
     3301 aaagttaaag catttttctt agcaatttct aaactatcta aacttttatc tattgctaca
     3361 acagtagagt ttttaaattt attttttact tcaattgcaa tacaaccaga accagtacca
     3421 atatcaataa tattattaaa ttggaatgga catgaagata ctgtggaatg tttgg
//
LOCUS       DAOQHP010000242         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17312_length_3473_cov_3.506144, whole genome shotgun sequence.
ACCESSION   DAOQHP010000242 DAOQHP010000000
VERSION     DAOQHP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17312_length_3473_cov_3.506144"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PLY81_07900"
     CDS             <1..1601
                     /locus_tag="PLY81_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNF30186.1"
                     /translation="KKKHKNKLAAEQKINAVLIKNLQQHVQYLASDSLEGRRTGTKGE
                     IAAMNYIVKQYTQEGIEPKGTTGYIQEFEINEGKKVDENSYLKINNVNLALHKDYFPL
                     ATSNNGKFTTSTAAIALKEISEPWFIDVKEELEANKNNPHFDINVYIKEEATNATKKG
                     ALALFFYNSSNITDNIMFNKNDPSAIEQIPIIYIKKEAIEKYFKDVSATFKLEYKIAI
                     APSIRKARNVVAYINNHAANTIILGAHYDHLGYGEDKNALDALHEVHNGADDNASGTA
                     ALIELAKMLKHSNAKNNNYLFIHFSGEELGLFGSKYWIENPSINITPNYMINMDMVGR
                     YDTAHKLTIGGYGTSPVWDNIFTTISNKNLILKFDSSGSGPSDHASFYRANIPVLFFF
                     TGSHSDYHKTTDDWEKINFTAQKDIIHLIYNIVDATNNKGKLAFTKTREPKVGKAKFS
                     VSLGVIPDYGYTGTGLRIEGVSPGKLAEKIGLQAGDVLLQLGEYKFVDVNSYMEALSK
                     FKKGDATKLTIKRGDKEMSFDIVF"
     gene            1625..2773
                     /locus_tag="PLY81_07905"
     CDS             1625..2773
                     /locus_tag="PLY81_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF30187.1"
                     /translation="MNIGFEAKRAYTNNTGLGHYSRTLITSLATYFANHQYYLFTPKL
                     SNFFNTSSFQNIQTIVPTSFPATLFTAIWRSSWVKKDLKKLNIQLYHGLSHEIPLGIQ
                     HTNIASVVTMHDLIFERYQEHYNSMDVKIYRNKFKNACKNSNKIIAISKQTKADLIDF
                     YKIEEEKIAICYQSCNPIFAQQETKETLETIKQFYQLPNKYFLYVGSIIERKNLLLIC
                     KALKMLQPAIKIPLVVIGEGGAYKKIIKQFIFSNGLENDVIFLSEKYTNDSFKTAKDF
                     PAIYQQAIALIYPSIFEGFGIPVLEALWSKTPVITSNVSCMPETGGDAAMYVNPYSEM
                     EMANAMEEIYKNDSLRESMIVKGISHAQNFTQEKCASEVFKVYKQIIN"
     gene            2776..3339
                     /locus_tag="PLY81_07910"
     CDS             2776..3339
                     /locus_tag="PLY81_07910"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF30188.1"
                     /translation="MNFNSDIEACLHILHNGGIILYPTDTIWGIGCDATNEDAVEKII
                     QLKQRVANKNFIVLVSDEKELMQYVAALDLEIFNYLETQKKPTTVIYHQAIGLANSVI
                     AEDGSVAIRICKDEFCKTLIKRFRKPIVSTSANISNQPSPKLFLDISNEIKNGVDYVV
                     AHKQNDQTPSTPSSIIEWKNGEIINVR"
BASE COUNT         1377 a          500 c          538 g         1058 t
ORIGIN      
        1 aaaagaagaa gcataaaaat aaacttgctg ctgaacaaaa aataaatgct gtactaatta
       61 aaaatcttca acaacatgtg caatatttag ctagcgattc tttagaagga agaagaacag
      121 gtacaaaggg tgaaattgct gcaatgaatt atattgttaa acaatataca caagaaggta
      181 ttgaacccaa aggcactact ggctatattc aagaatttga aattaatgaa ggaaaaaaag
      241 tagatgaaaa ttcttattta aaaattaaca atgtcaactt ggctctacat aaagattatt
      301 ttccacttgc aacaagtaat aatggaaaat ttacaacatc tactgcagca attgccttaa
      361 aagaaattag cgaaccttgg tttatagatg ttaaagaaga attagaagcc aataaaaaca
      421 atccacattt tgatattaac gtttatataa aagaagaagc aaccaatgcc actaaaaaag
      481 gagcattagc attgttcttt tacaattcct ctaatatcac agataatata atgttcaata
      541 aaaacgatcc atctgctatt gaacaaattc caattatata tattaagaaa gaagctattg
      601 aaaaatattt taaagatgta agtgcaacat tcaaattaga atataaaatt gctattgcac
      661 caagcataag aaaggccaga aatgtagtag cttatattaa caaccatgca gccaatacaa
      721 ttattcttgg agcacattat gaccacttag gatatggcga agataaaaat gctttagatg
      781 ctttacatga agtgcacaat ggtgcagatg ataatgctag tggcactgca gctttaatag
      841 agttagctaa aatgcttaag cattccaatg caaaaaataa taattattta ttcattcatt
      901 ttagtggaga ggagttagga ttgtttggta gtaaatactg gattgaaaac ccttctataa
      961 atattactcc aaactatatg attaatatgg atatggttgg taggtatgat acagcccata
     1021 aacttaccat aggagggtat ggaacatctc cagtttggga taatattttt accactataa
     1081 gtaataaaaa tttaattcta aaatttgata gctctggaag tggccctagc gaccatgcca
     1141 gtttttacag agcaaatatt ccagtattat tcttttttac aggaagccat tccgattatc
     1201 acaaaacaac agatgattgg gaaaaaatta attttactgc acaaaaagat attatacacc
     1261 taatttataa tattgttgat gcaacaaaca acaaaggcaa attagcattt actaaaacaa
     1321 gagaacctaa agtgggcaaa gcaaaattct ctgttagctt aggcgtaata ccagattatg
     1381 gctatactgg aactggttta agaatagaag gagtaagccc aggaaaatta gctgaaaaaa
     1441 ttggtttaca agccggagat gttttactac aattaggtga atacaaattt gtagatgtaa
     1501 acagctatat ggaggcatta agcaaattta aaaaaggaga cgcaaccaaa cttacaatta
     1561 aacgaggcga taaagaaatg agttttgata tagtgtttta aaaaataact taaatctgct
     1621 tataatgaat ataggttttg aggctaaaag agcctatacc aataatacag gtttaggaca
     1681 ttatagtcgt actttaatta cttctttagc aacttatttt gctaaccatc aatattattt
     1741 atttacacca aagctcagta atttttttaa tacttcatct ttccagaata tacaaaccat
     1801 tgtacccact agctttcctg caacattatt tacagctata tggcgtagca gttgggtaaa
     1861 aaaagattta aaaaaattaa atattcaatt gtatcatggc ttgagtcacg aaattccttt
     1921 aggtattcaa catactaata ttgcatctgt agttaccatg catgatttaa tttttgaaag
     1981 gtatcaagag cattataatt caatggatgt aaaaatttac cgaaataaat ttaaaaatgc
     2041 ttgtaaaaat tcaaataaaa ttattgccat aagcaagcaa actaaagccg atttaataga
     2101 tttttataaa atagaagaag aaaaaattgc tatttgttat caaagctgta accccatttt
     2161 tgcacaacaa gaaactaaag aaacgctaga aaccattaag caattttatc agctacccaa
     2221 caaatatttt ttgtatgtag gttccattat agaaagaaag aatttgttgc tcatttgtaa
     2281 agcattaaaa atgctacaac ctgccataaa aattccatta gtagtaatag gagaaggggg
     2341 agcatacaaa aaaattataa aacaatttat tttttcgaat ggcttagaaa atgatgtaat
     2401 ctttttgtca gaaaaatata ctaatgattc ttttaaaact gcaaaagatt ttcctgctat
     2461 ataccaacaa gcaattgcgt taatttaccc atctattttt gaaggatttg gaatacctgt
     2521 tttagaagca ttatggagta aaactcctgt aattacaagt aatgtatctt gcatgccaga
     2581 aactggtggt gatgctgcca tgtatgtaaa cccttacagc gaaatggaaa tggctaatgc
     2641 aatggaagaa atttataaaa acgatagttt aagagaaagt atgatagtaa aaggtatttc
     2701 tcatgctcaa aactttaccc aagaaaaatg tgcatccgaa gtttttaaag tatataagca
     2761 aataataaat taatagtgaa ttttaactct gatatagaag catgcttaca cattttacat
     2821 aatggaggca ttattttata cccaacagat accatttggg gtattggttg tgatgctact
     2881 aatgaagatg cagtggaaaa aattatacaa ttaaaacaaa gagtagcaaa taaaaatttt
     2941 attgtattag taagcgatga aaaagaacta atgcaatacg tagcagcctt agatttagaa
     3001 atatttaatt atttagaaac acaaaaaaaa ccaacaactg ttatttatca tcaagcaatt
     3061 ggtttagcaa atagtgtaat tgccgaagat ggctctgtgg ctataagaat ttgtaaagat
     3121 gaattttgta aaacacttat taaaagattt agaaaaccaa tagtaagtac atctgccaat
     3181 attagcaacc aaccttcccc aaaattattt ttagatataa gcaacgaaat aaaaaatgga
     3241 gttgattatg tggttgctca taaacaaaat gatcaaacac cctcaactcc atcttcaatt
     3301 attgaatgga aaaatggcga aataattaac gtacgttaat cattaaagta attaaatatt
     3361 atttctattt tgtttccttt tatctcttaa tttctttgct cctaaaccag ctcctgcagc
     3421 taacaataaa gttaaaccac catctacagg tatatcatct tcaggatcag gaa
//
LOCUS       DAOQHP010000243         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17333_length_3469_cov_4.683070, whole genome shotgun sequence.
ACCESSION   DAOQHP010000243 DAOQHP010000000
VERSION     DAOQHP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17333_length_3469_cov_4.683070"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="PLY81_07915"
     CDS             <1..1893
                     /locus_tag="PLY81_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF30189.1"
                     /translation="YLRHMHYVSAENLSKSFGVQPLFQNISFNINEGDKIALIARNGV
                     GKSTLLKILAGKETTDTGKIYINKEVTTVLFEQNPAFEEDKNIVDNIFKANHPIVNAI
                     RAYELALEENNNEELLRLATLMDELNAWNFEVKVKQILTKLNIHHLQQTVKNLSGGQR
                     KRIALAKTLIEIGFEHTHTLLLMDEPTNHLDVEMIEWLEKYLNQENVTLILVTHDRYF
                     LDAITDEIWELENDNLYIYKGDYENYIEKKAARIESDLASIDKAKNQYRKELEWMRKQ
                     PKARTTKSKSRQDNFYEIEAKAKQKIEDAQVQLQVKMSRLGGKIAEMKKVYKSYGNNI
                     ILNGFDYTFNKGERIGIIGKNGVGKSTFLNILQQLEKADSGKVNIGDTIVFGNFSQQG
                     LEITENLRVIEYVKTFAENFPLANGGSLSAAQFLELFLFTPDKQYTYISKLSGGEKKR
                     LQLLIVLFKNPNFLILDEPTNDLDLPTLSVLEQFLETYQGCVIIVSHDRYFMDKLVDH
                     LFIFEGNGIIKDFPGTYTQYRIWQKEQDKTEEKVVVKPTQTSNSTNQTNINKKKASFK
                     EKREFEILEKEISALEKEKEALTTELSNTTNNYETIEKISTRLVEINNHLELKEMRWL
                     ELSELL"
     gene            complement(1928..2001)
                     /locus_tag="PLY81_07920"
     tRNA            complement(1928..2001)
                     /locus_tag="PLY81_07920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:Arg,seq:acg)
     gene            complement(2077..2586)
                     /locus_tag="PLY81_07925"
     CDS             complement(2077..2586)
                     /locus_tag="PLY81_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30190.1"
                     /translation="MKITSIYQVLNFLLLPFALLLGFTAFPMIIAALANATALLPLFM
                     IVCIIIYIYCSIRFFNRGVKQQQICNKSLKDWIKVNAYVCIAFVLLMFVQGSVIAANP
                     MLISDQLMADLAKQSNNSLPPQVNAALVGKMLKYVTYFMLFYSVVLSVHIFMSFKLIK
                     TNNHLFQEQ"
     gene            2709..3074
                     /locus_tag="PLY81_07930"
     CDS             2709..3074
                     /locus_tag="PLY81_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF30191.1"
                     /translation="MKILIAEDELLMAKTLEKKLLLDGHEVLIANDGRTALTLYETEN
                     PDLIITDIMMPYTSGLELVGIVKANLNKKTPIIVLSGMGEENIVLQAFEMGADDFLTK
                     PFKPSELSLRVKRLEMKLR"
     gene            3174..3247
                     /locus_tag="PLY81_07935"
     tRNA            3174..3247
                     /locus_tag="PLY81_07935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3208..3210,aa:Ile,seq:cat)
BASE COUNT         1390 a          459 c          546 g         1074 t
ORIGIN      
        1 tatcttcgcc acatgcatta tgtatcagcc gagaatttaa gtaagagctt tggtgttcag
       61 cctttatttc aaaatatttc atttaatatt aacgaaggag ataagattgc tttaatagcc
      121 agaaatggag ttgggaaaag cacattatta aaaattttag caggtaaaga aactaccgat
      181 actggaaaaa tttatattaa taaagaagta actactgttt tatttgaaca aaatcctgct
      241 tttgaagaag acaaaaatat tgtagataat atatttaaag ctaaccatcc tatagtaaat
      301 gctataagag cttatgaact tgcattagaa gaaaataata atgaagaatt attaagactt
      361 gcaaccttaa tggatgaatt gaatgcatgg aattttgaag taaaagtgaa gcaaatttta
      421 acaaaactaa atattcatca tttacaacaa acagtaaaaa atttaagtgg cggacaaaga
      481 aaaagaattg ctttagctaa aacattgatt gaaattggct ttgaacatac ccacactttg
      541 ctattgatgg atgaaccaac taaccattta gatgttgaaa tgattgaatg gttagagaaa
      601 tacttaaatc aagaaaatgt aactcttata ctggtaacac acgatagata ttttttagac
      661 gctattactg atgaaatttg ggaactagaa aatgataatt tatatattta taaaggagat
      721 tatgaaaatt atatagaaaa aaaagcagcc cgtatagaaa gtgatttagc aagtatagac
      781 aaggctaaaa accaatatag aaaagagtta gaatggatgc gtaagcaacc aaaagcaaga
      841 acaacaaaaa gcaaaagcag gcaagataat ttttatgaaa tagaggcaaa agcaaagcaa
      901 aaaatagaag atgctcaagt acaattgcaa gtaaaaatga gccgtttagg tggcaaaatt
      961 gccgaaatga agaaagtata taaaagctac ggtaacaata ttattttaaa tggttttgat
     1021 tatactttta ataaaggaga acgaattgga ataataggta agaatggcgt agggaaaagt
     1081 acctttttaa atattttgca gcaattagaa aaagcagata gtggtaaagt aaatattggc
     1141 gataccattg tttttggtaa tttttctcaa caaggtttag aaataactga aaacttaaga
     1201 gtaatagaat atgtaaaaac atttgccgaa aattttcctt tagctaatgg tggctcatta
     1261 agtgcagcac aatttttaga actattttta tttacaccag ataaacaata cacctacatt
     1321 agcaaattaa gcggaggcga aaaaaaacga ttacaattat taatagtatt atttaaaaat
     1381 cctaactttt taattttaga tgagccaacc aacgatttag atttacctac tttatctgta
     1441 ttagaacagt ttttagaaac ttatcaaggt tgtgtaatta ttgtaagcca tgataggtat
     1501 tttatggata aattggtaga tcatttattt atttttgaag gcaatggaat catcaaagat
     1561 tttcctggaa cctatacaca atatagaatt tggcaaaagg aacaagataa aacagaggag
     1621 aaagttgttg taaaacctac tcaaaccagc aactcaacta accaaaccaa tattaacaaa
     1681 aagaaagcaa gctttaaaga aaaaagagaa tttgaaatat tagaaaaaga gatttcggct
     1741 ttagaaaaag aaaaagaagc ccttactaca gaactaagca acacaacgaa taactatgaa
     1801 acaattgaaa aaataagtac ccgattagta gaaataaata accatttaga actaaaagaa
     1861 atgcgttggt tagaactgag tgaattatta taagaataaa aaagctgcta attaaagcag
     1921 cttttagtga ccccgtcagg attcaaacct gaaacctttt gatccgtagt caaatgctct
     1981 attcagttga gctacggagc catttatatc ccatttaagg gctgcaaata taaacacttg
     2041 ttgctaaaaa aaacaaatta aattaattta gaaactctat tgctcttgaa ataaatgatt
     2101 gtttgttttt attagtttaa aactcataaa aatatgtaca ctaagtacta cagaataaaa
     2161 caacataaaa taggtaacat attttagcat tttaccaact aatgcagcat ttacttgagg
     2221 aggcaggcta ttgttacttt gtttggctaa atctgccatt aactgatcgg aaattaacat
     2281 tgggtttgca gcaattacac ttccttgcac aaacatcaac aacacaaatg caatacatac
     2341 ataagcgttt actttaatcc aatcttttaa agatttatta caaatttgtt gctgctttac
     2401 tcccctatta aaaaacctta tgctacaata aatgtaaata attatacaaa ctatcataaa
     2461 caatggtagt aaagcagtag catttgctaa agcagcaatt atcataggaa aagcagtaaa
     2521 acctaaaagc agagcaaagg gtaataataa aaaatttaat acttgatata tagatgtaat
     2581 tttcatttag ttattttatt aaaataaaaa attgatacaa taattttata ttaatgatat
     2641 ttgattaata gattgtattt tacaaataaa gaaaattatt tttactttta gctcaacccg
     2701 tatataatat gaaaatttta attgccgaag atgaactttt aatggctaaa actttagaga
     2761 aaaaactttt attagatggg catgaagttt taatagcaaa tgatggaaga actgcattaa
     2821 ctctttatga aacagaaaat ccggatttaa ttattacaga tattatgatg ccatatactt
     2881 ctggcttaga attagtaggc attgtaaaag ccaatttgaa taaaaaaaca ccgatcattg
     2941 ttttatctgg tatgggagaa gaaaatattg tattacaagc ttttgaaatg ggtgctgacg
     3001 attttttaac taaaccattt aaaccttctg aactttcttt aagggtaaaa agattagaaa
     3061 tgaaattaag gtaatatgtt tcaaataatt tctaaaaaat aatattttct tcttggttgg
     3121 taatgttaaa actttatttt tgccgtcctt aatagaatct gaaagaagat tctggaccta
     3181 tagctcagtt ggttagagca cctgactcat aatcaggggg tccctggttc aagcccaggt
     3241 gggtccacaa gtaaatcaaa agccatttaa gcatttaact taaatggctt ttaaatttaa
     3301 tagaactact tacagtattt gtatttactg ttatgaatta ggaatggttt ttaatttaaa
     3361 cttctaaaat ctactggtac caatttgctc acaccaggct cttgcatagt tacaccataa
     3421 attacatcca ccgagcccat tgtttgcttg ttgtgtgtta caataataa
//
LOCUS       DAOQHP010000244         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17544_length_3445_cov_3.274631, whole genome shotgun sequence.
ACCESSION   DAOQHP010000244 DAOQHP010000000
VERSION     DAOQHP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17544_length_3445_cov_3.274631"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1075)
                     /locus_tag="PLY81_07940"
     CDS             complement(239..1075)
                     /locus_tag="PLY81_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30192.1"
                     /translation="MNVLKLFSVIAVITLFISCKKENTIIPNDTDGLISAKSISNATH
                     NIIFYTQNGKFQQGYNKIFFQIKNLDGSLVTNATANWKPLMHMTSMQHACPYSSVSKS
                     ANSNSLYEGFIVFQMAGNSTEYWDLTIEYTIDNTTYTATERIDVEAVTKKNVLSFKGS
                     DNVSYVLAMVAPSNPKVATNDMEAMLFKKETMMSYPVVNNYTIKIDPRMTSMGNHGSP
                     NNVDLKQTSLNGSYFGKLSLTMTGYWKINLQLENESGTILKGEPVTATQESSSIFFEI
                     EF"
     gene            complement(1153..1479)
                     /locus_tag="PLY81_07945"
     CDS             complement(1153..1479)
                     /locus_tag="PLY81_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30193.1"
                     /translation="MKNSILVGFYELNTKAFVSLFCENKSKPELKCNGKCQLSQIAKQ
                     EERNTHHNLIIQLQQEVVLYFHQPLNPFIENTFYNIKASFNLFQQHNYSFLYLSKNDK
                     PPKFLS"
     gene            1681..1836
                     /locus_tag="PLY81_07950"
     CDS             1681..1836
                     /locus_tag="PLY81_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30194.1"
                     /translation="MKKTSKLLAVIAAEFLMIALFVPIWNIYLDAPKYTEGLVLKIWA
                     NYLRNYC"
     gene            1948..2163
                     /locus_tag="PLY81_07955"
     CDS             1948..2163
                     /locus_tag="PLY81_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30195.1"
                     /translation="MNGSLEKYRNISYTKIKQVTVADTAGRMVRNMQVNGTDMMQVNI
                     AGLQQGVYIIKVISVVGITESTKLIVN"
     gene            2175..>3445
                     /locus_tag="PLY81_07960"
     CDS             2175..>3445
                     /locus_tag="PLY81_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30196.1"
                     /translation="MIIRVYLVTLFCCSIFCSKAQQQYNLVPNYSFELYENCPTSYEL
                     ADALHDKPSYWYKPDKRGARYFNACANGFSNNNNGIPVNLAGGGYNYQYARTGNGYVA
                     MFYYNHMDTRNYIQVQLLDSLRQGKCYYAEYYVNLINSINFGCNNQSMLFTNTPIYAD
                     TATGLQIIPANPQVQHPAIVTDTLNWVKVSAVFTAQGGEKFLTLGNFKNNAQTATQLI
                     QTTGYSGAAYYIEDVSVIPLDSITLKADAGTDKTITVGDSVFIGSFTTGLTNVNWYNA
                     TGNIIATGIPGMYVKPATSTFYVIEQTVCGQYSKDTVYVSVGVVPLVIKHYELKIKNE
                     GVVNKWITLNEINVSHFNVQRSSNGIEFNTIQKTTAKNNTYNEYSITDVQPLNGVSYY
                     RIEAVDKDGKITYSKTEKVQLKVDKEQLTVFP"
BASE COUNT         1204 a          498 c          569 g         1174 t
ORIGIN      
        1 tgctgtactg caaccacaac ttctgctaac tgatgtgtgc tatctgtaaa ataagatgct
       61 tgctgttgtg catttgactg atgaaataat aatatcaata aactgataac aaccgataat
      121 ttattcatga ttatttattt ttaaagaatt gtttgataca accaatttgc tataccacac
      181 ttgattttta tatcaaacaa ttcttttgta aaacaatttc tattttttag gaattgtatt
      241 aaaactcaat ttcaaaaaat atactgctgc tttcttgagt tgcagtaaca ggttctcctt
      301 ttaaaattgt accactttcg ttttctaact gaaggtttat tttccaataa cctgtcatag
      361 taagacttaa ttttccaaag taagaaccat tcaaagaagt ttgttttaaa tctacattat
      421 taggtgaacc atgattaccc atacttgtca ttcttgggtc tatttttatt gtataattat
      481 ttactacagg ataactcatc attgtttctt ttttaaaaag cattgcttcc atatcattgg
      541 tagcaacttt agggtttgat ggagcaacca ttgctaaaac ataactcaca ttatcgctgc
      601 ctttaaacga caatacattt tttttagtta ctgcttctac atcaattctt tctgttgcag
      661 tgtaagtagt gttgtcaatg gtgtattcta tagttaaatc ccaatattca gtactgtttc
      721 cagccatttg aaaaactata aaaccttcgt acaaagaatt gctgttggca gatttagaaa
      781 cacttgaata agggcaagca tgttgcatac ttgtcatgtg cattagaggc ttccaatttg
      841 ctgtagcatt ggtaactaaa cttccatcaa ggttttttat ttgaaaaaat attttattat
      901 acccttgctg aaattttcca ttctgggtat aaaaaataat gttgtgtgta gcatttgaaa
      961 tacttttcgc tgaaatcaaa ccatcggtat cgttaggtat tattgtgttt tcttttttac
     1021 aagatataaa tagagttatt acagctataa cgctgaatag ttttaataca ttcatttgaa
     1081 ttaaattttt aaattaatag aatagattat cataccacat aagctgtatg atgaaaaata
     1141 aatagagagc tattaactta aaaacttagg aggtttatcg ttttttgata aataaagaaa
     1201 ggaataattg tgttgttgaa ataaattgaa tgaggcttta atattgtaaa atgtattttc
     1261 aataaaaggg tttagaggtt gatgaaaata caatacaact tcttgttgta gctgaattat
     1321 taaattatgg tgtgtatttc tttcttcttg tttagcaatt tgtgatagct gacatttacc
     1381 attacatttt agttcgggct ttgatttatt ttcgcaaaat aaacttacga atgcttttgt
     1441 atttaattca taaaagccaa ctaaaatact attcttcata atgctaaaaa acattagtaa
     1501 gaataataag agtgttacac ctttgcgaaa tagtaacaat gtagttgaat tttattttgc
     1561 tgcaaaaata gtagtaaaat tttgttcttt gaatgattat aatcatagac ttttatgtat
     1621 tgtaaaaata tctttctcca ttaatcaaaa agttaattat tgtaaaatga aaaatgttat
     1681 ttgaagaaaa catcaaaact tttagctgtt atagctgcag aatttttaat gattgctctt
     1741 tttgtcccta tttggaatat ttatttagat gcaccaaaat atactgaagg tttagtgttg
     1801 aaaatttggg caaattattt aagaaactat tgctaagaca catccattaa ttttaaaatc
     1861 caatcacaat taattttact atataatcca ctatggatta ttttatgaca gttaaatctt
     1921 ttttcgtacc ctaaaaatta cctaagtttg aatggcagtt tagagaagta tagaaatatt
     1981 agctatacca aaattaaaca agtaactgtt gcagatactg cagggcgtat ggtaagaaat
     2041 atgcaggtta acggaacaga tatgatgcaa gtaaatattg caggtttgca gcaaggggta
     2101 tatataataa aagttatttc agttgtaggt attacagaaa gtacaaaact aattgttaac
     2161 taataaacta acctatgatt attagagtat atcttgtaac attgttttgt tgctctattt
     2221 tttgttctaa agcacagcaa caatacaatc ttgtgcctaa ctatagtttt gaactatacg
     2281 aaaattgtcc cacaagttat gaattggcag atgccttgca cgataaacca agttattggt
     2341 acaagcccga taagcgtgga gcaaggtatt ttaatgcctg tgccaatggt tttagcaaca
     2401 ataataacgg aatacctgta aatcttgcgg ggggggggta caattatcaa tatgccagaa
     2461 ccggaaatgg ctatgttgcc atgttctatt ataatcacat ggacacaaga aattatatac
     2521 aggtacaatt attagatagt ttaaggcagg gaaaatgtta ctatgcagag tattatgtaa
     2581 atctaataaa ttcgataaat tttggctgca acaaccaatc catgttgttt acaaataccc
     2641 ctatttatgc agataccgca acaggcttac aaattattcc tgccaaccca caagtacagc
     2701 atcctgccat tgtcaccgac accttgaact gggtgaaggt gagtgctgtt ttcactgccc
     2761 aaggaggtga gaagtttttg acactgggca atttcaagaa caatgcacag acggctacac
     2821 aactaattca aaccaccggg tactctggtg ctgcttacta tatagaagat gtaagtgtaa
     2881 tacctttaga tagtataacc cttaaagcag atgcaggtac agataaaacc ataacggtag
     2941 gagatagtgt ttttataggc agttttacta caggacttac caatgtaaat tggtataatg
     3001 caacaggcaa tataatagca acaggcatac ccggtatgta tgtaaaacca gcaacaagta
     3061 ctttttatgt aatagagcaa acggtttgtg ggcagtactc caaagacact gtgtatgtga
     3121 gtgtaggggt agtgcctttg gtaattaagc attatgaatt aaaaattaag aatgaaggcg
     3181 tagtaaataa atggattact ttaaatgaaa tcaatgtttc acattttaat gtacaacgta
     3241 gcagtaatgg tatagagttt aataccatac aaaaaacaac agcaaaaaat aatacttata
     3301 atgaatacag cattacagat gtgcagcctt taaacggagt aagttattac agaatagaag
     3361 cagtagataa agatggcaaa ataacataca gtaaaacaga gaaagtgcaa ttgaaagttg
     3421 ataaggaaca attgacagtt tttcc
//
LOCUS       DAOQHP010000245         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17720_length_3420_cov_4.093016, whole genome shotgun sequence.
ACCESSION   DAOQHP010000245 DAOQHP010000000
VERSION     DAOQHP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17720_length_3420_cov_4.093016"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLY81_07965"
     CDS             <1..311
                     /locus_tag="PLY81_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30197.1"
                     /translation="RLYPIPSFFLLAQPEHNWIKVTQTNLDQPGVPKITQKVQASSFL
                     VGVGYGQRIIGSSSFFTTIMFDLGKEIYSPYRDGFNNAIPIVRTGFNWYLNSAKKKKK
                     "
     gene            complement(292..828)
                     /locus_tag="PLY81_07970"
     CDS             complement(292..828)
                     /locus_tag="PLY81_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF30198.1"
                     /translation="MNWKKVAVFCGSKLGNNQLYYQHAVELGKLLAEHKIQMVYGGGK
                     VGIMGAIADAVMEYNGTVTGIIPELLVEWEQQHENISQLHVVKDMHIRKKMMYELCDA
                     VIVLPGGYGTMDELFEVITWNNLKIHEKKTILLNTAGYYNHLIAHIDTMAKEGFLYNN
                     WNENIIVANNPAAIFSSF"
     gene            complement(844..2685)
                     /locus_tag="PLY81_07975"
     CDS             complement(844..2685)
                     /locus_tag="PLY81_07975"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNF30199.1"
                     /translation="MAYASLEECLIDLEQHHHLIKINEEVDPYLEMAAIHLRVHEMNG
                     PALLFNNVKGTKFRAASNLFGNIERSKFIFRDTFKTVQELIEIKNNPINVLKKPFKNI
                     KAGIAALKAFPLKNPIHKPVLYQEINISDLPHIHHWKKDGGAFITLPQVYTEDIEQPG
                     VMHSNLGMYRIQLTGNDYVLNKEIGLHYQLHRGIGIHQTKANKKGCSLKVSCFVGGPP
                     AHTLSAVMPLPEGMSELTFAGVLGARRFRYIYQNEFCISTDADFVITGEVIVNENKPE
                     GPFGDHLGYYSLTHDFPVLKVNKVYAKKNAIWPFTVVGRPPQEDTSFGALIHELTGSA
                     LKNEISGVKEIHAVDAAGVHPLQLAIGSERYTPYLPSKQPAELLTIANHILGTGQLSL
                     LKFLFITADDTNNLSTKNIQSFFTYVFERIDYRRDIHFYTNTTIDTLDYSGTSLNTGS
                     KVVFAAYGDVIRNLCKEVPSCFYGLQNFANPQIVIPGVIALKAKTFTTYENAKQEMEQ
                     LNSYLYSYSSELKDVAQIIVCDDSNFLSEKLNNYLWVTYTRCNPSHDIYGVAEFIEYK
                     HWGCTSSVILDARIKPHHAPLLEKDEATERKIDRLFEKGGSLYSLIK"
     gene            complement(2864..>3420)
                     /locus_tag="PLY81_07980"
     CDS             complement(2864..>3420)
                     /locus_tag="PLY81_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNF30200.1"
                     /translation="LVEEMINISKQKTEELGKVSDFSSVELDELNKVIGLGALKFFLL
                     RVDPKKKMIFDPVESIDFHGFTAPFIQYTHARIKSILRKNSITNNASSNSKLLPLEKE
                     VVRHLELFNATLLEAAKEHDPSKIAIYVFNLAQVFNSFYAQHSIAHAENENQKLLRLQ
                     IASLTAHVIKKGMHVLGIEVPERM"
BASE COUNT         1104 a          599 c          455 g         1262 t
ORIGIN      
        1 taaggcttta tcctattcct agtttttttc tattagcaca gcctgaacat aactggataa
       61 aagtaactca aactaattta gatcaacctg gtgttcctaa aattactcaa aaagtacaag
      121 catcaagttt tttggttggc gtaggttatg gacaacgaat aataggcagt tccagttttt
      181 ttaccactat tatgtttgat ttaggaaaag aaatttattc tccttataga gatggtttta
      241 ataatgctat tcctattgtt cgtacaggat ttaactggta tttaaactct gctaaaaaga
      301 agaaaaaata gctgcaggat tgttagcaac aataatattt tcattccaat tattatacaa
      361 aaaaccttct tttgccattg tatcaatatg tgcaattaaa tggttgtaat agcctgcagt
      421 attaagcaat attgttttct tttcatgtat ttttaaattg ttccaggtaa ttacttcaaa
      481 caactcatcc atagtaccat aaccaccagg cagtactatt acagcatcgc ataactcgta
      541 catcattttc tttctaatat gcatgtcttt taccacatgc aattgactga tattttcatg
      601 ctgttgctcc cactctacca aaagctcagg aatgatgcca gtaacagttc cattatactc
      661 cattactgca tcagcaatgg cacccattat tcctacttta cctccaccat aaaccatctg
      721 tattttgtgc tctgctagta atttgccaag ctctactgca tgttgataat ataattgatt
      781 attacctaat ttacttccac aaaaaacagc aacctttttc caattcataa acaacctaat
      841 tttttatttt attaaactat ataaacttcc acccttttca aataatctgt caattttcct
      901 ttctgtagct tcgtcttttt ctaataacgg tgcatgatgt ggtttaattc ttgcatccaa
      961 aattacagaa cttgtacatc cccaatgttt atactcaata aactcagcaa ctccgtaaat
     1021 atcatgactt ggattacaac gtgtgtaagt tacccataaa taattattca atttttctga
     1081 taagaaatta ctgtcatcac aaacaattat ttgtgcaaca tcttttaact cactgctgta
     1141 agaatataga tatgaattta actgctccat ttcttgttta gcattttcat aggttgtaaa
     1201 agtttttgct tttaaagcaa taactccagg aattacaatt tgtggattag caaagttttg
     1261 aagcccataa aaacaactag gaacttcctt acacaaattt ctaattacat cgccataggc
     1321 tgcaaataca actttgctgc ctgtatttaa gcttgtacca ctataatcca aagtatctat
     1381 tgtagtattg gtataaaaat gaatatctct cctataatca attctttcaa aaacataggt
     1441 aaaaaaggat tgaatatttt ttgtagaaag attattggta tcatcggctg taataaataa
     1501 aaactttaat aaacttagtt gccctgtacc taaaatatga ttggcaattg ttaataattc
     1561 tgctggttgt ttggaaggca aataaggcgt atatctttcg ctacctatag ctaattgtaa
     1621 aggatgtaca ccagcagcat ccacagcatg aatttctttt acaccactaa tttcattttt
     1681 taatgcacta cctgtaagct catgtattaa agccccaaaa ctagtatctt cttgtggtgg
     1741 cctgccaact actgtaaaag gccatattgc atttttttta gcgtaaactt tattgacttt
     1801 taaaaccgga aaatcatgtg ttaatgaata ataacctaaa tgatctccaa aagggccttc
     1861 gggtttattt tcattaacta taacttcacc agtaattaca aaatctgcat cggtacttat
     1921 acaaaattca ttttgataaa tgtacctaaa tcttcttgca cctaaaactc ctgcaaaagt
     1981 taattcactc ataccttctg gcaatggcat tacagcactt aaagtatgag caggaggccc
     2041 accaacaaaa caactaactt ttaacgagca acctttttta tttgctttgg tttggtgaat
     2101 accaatgcca cgatgtaatt gatagtgcaa accaatttct ttattcaaca cataatcatt
     2161 accagtaagt tgaatacgat acataccaag gttagagtgc attacacctg gctgttctat
     2221 gtcctcggta taaacttgag gtaatgtaat aaatgctcct ccatcttttt tccaatgatg
     2281 aatatgtggt aaatcgctaa tattaatttc ttggtataaa actggtttgt gaataggatt
     2341 ttttaatgga aatgctttta aggctgcaat tcctgcttta atatttttaa aaggtttctt
     2401 taaaacattt attggattgt ttttaatttc tattagttct tgaactgttt taaatgtatc
     2461 tctaaatata aacttactcc tttcaatatt accaaatagg tttgatgcag ctctgaattt
     2521 tgttcctttt acattattaa aaagtaatgc agggccattc atttcgtgta ctcttaaatg
     2581 tatggcagcc atttctaaat aaggatccac ctcttcattt atttttatta aatgatgatg
     2641 ttgttctaaa tcaatcaaac actcttctaa actagcgtaa gccatgctac agttttttgt
     2701 ttatgcaata aaagtaaagt atattttaat tcagaattaa aagaacagag aaactaaata
     2761 gttaactgca ttcaattata aaaaatagga ttagcgtatt ttataagttg gaagtaaatt
     2821 tagttttatt acttaactat tgctttacct aatagaatat tttttacatt ctttctggaa
     2881 cttcaatacc taaaacatgc ataccttttt taattacatg tgccgttaaa gatgcaattt
     2941 gcaatcttaa tagtttttgg ttttcatttt cagcatgagc tatggaatgt tgtgcataaa
     3001 agctattaaa tacctgtgct aaattaaaaa cataaatagc aattttactt gggtcatgtt
     3061 ctttagcagc ttctaataat gttgcattaa aaagttctaa atgcctaaca acttcttttt
     3121 ctagtggtag taattttgaa ttactacttg cattattcgt tattgaattt tttcttaaaa
     3181 tactttttat tctggcatgt gtatattgta taaaaggtgc agtaaaccca tgaaaatcta
     3241 tactttctac tgggtcaaaa atcattttct ttttggggtc cactctaagt agaaagaatt
     3301 ttaatgcacc tagcccaatt actttattaa gttcatctaa ttctacacta ctaaaatcgc
     3361 ttaccttccc taattcttct gttttttgtt ttgatatgtt tatcatttct tctactaaat
//
LOCUS       DAOQHP010000246         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17838_length_3405_cov_4.018507, whole genome shotgun sequence.
ACCESSION   DAOQHP010000246 DAOQHP010000000
VERSION     DAOQHP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17838_length_3405_cov_4.018507"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="sprA"
                     /locus_tag="PLY81_07985"
     CDS             complement(<1..1146)
                     /gene="sprA"
                     /locus_tag="PLY81_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF30201.1"
                     /translation="MFAQTNKSTDTSKKVNNDSLHYPIKDRWGDRYTWQNKNPFNIRD
                     TSLLKQQIEYDPITKRYYIIEKIGSKTYRTPTYLTFEEFQKIQNYQAEVEYFKHRAQA
                     LTSLNRKVLRPKMRVFDKLFDRIFGLGKDGFKVDIKPQGSVDILAGYQGQITKNPTLP
                     EAARKNGGFDFDMNANLNVNANIGDKLKLPISYNTLANFDFENQLKLDYKGMDDEILK
                     SVEAGNISWQSKGTLIPSTQNLFGLKTQLQFGKLFLTGAIANQRSIRQSQSLQGGSAT
                     TPFQKKLDDYEENRHFLLGQYFKNNFNTAMRNLPVVNSQVQIQRIEVWVTNRTGATTD
                     ARDVVGFADFGESNPSNAVNLPGNGTPSGLPGNQANALYNKLIAGGMG"
     gene            complement(1263..1844)
                     /gene="ruvA"
                     /locus_tag="PLY81_07990"
     CDS             complement(1263..1844)
                     /gene="ruvA"
                     /locus_tag="PLY81_07990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNF30202.1"
                     /translation="MIAFIRGSFIQKQPARVVVDVNGIGYDLQISLNTYSYIANKDQG
                     QLFTYLHITENAQTLYGFADLEEKELFLNLISVSGVGAATARMMLSGMKPEEIARAIV
                     QGNTKQLESIKGIGKKSAERLIVELKDKLSKNASTALTTITNALPGYDAVNALVSLGI
                     AKPMAEQAVKKVLSSTPEINNIEELIKNALKNL"
     gene            1993..2982
                     /locus_tag="PLY81_07995"
     CDS             1993..2982
                     /locus_tag="PLY81_07995"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNF30203.1"
                     /translation="MNKITAAITAVGGYLPETKLTNADLEKMVDTTDEWIKTRTGIHE
                     RRILKEPGKANSDMIVPAIQEILNKKGLSPLDIDCIICATVTPDYVFPATANVVSDKL
                     GATNAWGYDLGAACSGFLFALTTGAALIESGRYKKVVVAGADKMSSIIDYTDRNTCII
                     FGDGAGAVLLEPNSEGNGVLDSILKSDGSGREFLHMKAGGSYSPATIETVMAKQHFVY
                     QEGKTVFKFAVKGMADVSAELLERNNLTGNDIAWLVPHQANLRIIDATAERVGLPKEK
                     VMINIQRYGNTTSGTIPLCLWEWEKQLKKGDNLVLAAFGGGFTWGAILVKWAY"
     gene            3033..>3405
                     /locus_tag="PLY81_08000"
     CDS             3033..>3405
                     /locus_tag="PLY81_08000"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF30204.1"
                     /translation="MMRDLNWKTLSSKYIYKDRWFIARADSCLMANGKTIEPYYVLEF
                     PNWCNVIVVTNKEEIVMVRQYRHAINKTTIELPGGVIDKNENPKEAAIREVLEETGYT
                     IHQIEFLYATAPNPATNNNMAY"
BASE COUNT         1110 a          531 c          577 g         1187 t
ORIGIN      
        1 tcccatacca cctgcaatta atttgttata caaagcattt gcctggttgc ctggtaaacc
       61 acttggtgtg ccattgcctg gtaagtttac agcattcgat gggttacttt cgccaaaatc
      121 tgcaaaacct actacatctc ttgcatctgt tgttgctcct gttctatttg ttacccaaac
      181 ttcaatacgt tgtatttgta cctggctatt tacaactggt aaattacgca ttgcagtatt
      241 aaaattattt ttaaaatact gacctagtaa aaaatgtctg ttctcttcat aatcatctaa
      301 ctttttttga aaaggagtgg ttgcggaacc gccttgtaaa ctttggcttt gccttatgct
      361 tctttgatta gctattgcac ctgttaaaaa taatttacca aattgtaatt gcgtttttaa
      421 tccaaataaa ttttgtgtgc ttggtattaa tgtacctttt gattgccatg aaatattgcc
      481 agcttccaca cttttcaata tttcatcatc catgcctttg tagtctaatt tcaactggtt
      541 ttcaaaatca aaattagcaa gggtattata gctaattggt aatttaagtt tatcgccaat
      601 atttgcattt acatttaaat tggcattcat gtcaaaatca aagccaccat tttttcttgc
      661 tgcttcgggc aatgttggat ttttggttat ttgaccttga taccctgcta aaatatctac
      721 agagccttgt ggtttaatat ctactttaaa accatctttt cctaaaccaa aaattctatc
      781 aaaaagttta tcaaaaaccc tcattttagg tcttaatact tttctgttta aagaggttaa
      841 agcttgagca cggtgtttaa aatattctac ttcagcctgg tagttttgta ttttctgaaa
      901 ttcttcaaaa gttaaatagg taggagttcg gtaagtttta ctaccaattt tttcaataat
      961 ataatacctt tttgttatag ggtcgtactc tatttgttgt ttaagtaagg atgtatctct
     1021 aatattaaat gggtttttgt tttgccaggt atatctatcg ccccatctat cttttatagg
     1081 ataatgtaaa gagtcgttat ttactttctt tgaagtatct gtacttttat tggtttgtgc
     1141 aaacacagca gtgctactgc atagaaatgc aaaaaatacg aacagccttt taaggcaatt
     1201 attatataga gtaaaataca aaattcctac accttggtta agtaaaaaat caaatctata
     1261 aactacaaat tttttaaagc gtttttaatt aattcttcaa tattatttat ttcaggggtt
     1321 gaggataata cttttttaac agcttgttct gccataggtt ttgcaatacc taaggatact
     1381 aatgcattaa cagcatcgta cccaggtaat gcattggtta tagtagttag agctgttgaa
     1441 gcatttttgc tcagtttatc ttttaactct acaattaatc tttctgcact ttttttacca
     1501 attcctttga tactttctaa ttgtttagtg ttgccctgta caattgctct agctatttct
     1561 tctggcttca taccagacaa catcattctt gcagttgctg cacctacacc agatacactt
     1621 attaagttca aaaacaattc tttttcttcc aaatctgcaa aaccataaag cgtttgtgca
     1681 ttttcggtaa tatgtaaata tgtaaacagt tgcccttggt ctttattggc tatataagaa
     1741 taagtattta aacttatttg caaatcgtag ccaataccat ttacatcaac caccactcgg
     1801 gcaggttgtt tttgaataaa acttcctcta ataaatgcta tcataattta caaggatttc
     1861 aaaaataagg gtttatataa tatatgaaca taaatttgta gttaaaacat tttacgtaaa
     1921 acaatattcc tattctctta agtaaatatg gatatttgtt acttaaattt ataattagaa
     1981 ataaataatt aaatgaataa aattacagca gctattacag cagttggagg ctatttgcca
     2041 gaaacaaaac taaccaatgc cgatttagaa aaaatggtag atactaccga cgaatggatt
     2101 aaaaccagaa caggtattca tgaaagaaga attttgaaag aacctggcaa ggcaaatagt
     2161 gatatgattg tgcctgcaat tcaggaaata ttaaacaaaa aaggactaag ccctttagat
     2221 atagattgta ttatttgtgc tactgtaaca ccagattatg tttttcctgc aacagcaaat
     2281 gtagtttctg ataaattagg agctaccaat gcctggggat acgatttagg ggcagcttgt
     2341 agtggctttt tatttgcatt aacaactggt gctgcattaa ttgaaagtgg tagatataaa
     2401 aaagtggttg tagctggtgc agataaaatg agctccataa tagattatac tgatagaaat
     2461 acatgtatca tttttggtga tggtgctggt gctgttttat tagaacccaa tagtgaagga
     2521 aatggagtgt tagatagtat tttaaaaagt gatggatcgg gccgtgagtt tttacacatg
     2581 aaagcaggag gctcttacag ccctgcaact atagaaacag taatggcaaa acaacatttt
     2641 gtgtaccaag aggggaaaac agtttttaaa tttgcagtaa aaggaatggc tgatgtgagt
     2701 gctgaacttt tagaaagaaa taacttaaca ggcaatgata tagcatggtt agtacctcat
     2761 caagctaacc ttagaattat tgatgcaact gcagaacgtg taggtttacc taaagaaaaa
     2821 gtgatgatta acatacaacg ttatggtaat accacttctg gcactatacc tttatgttta
     2881 tgggagtggg aaaagcaact taaaaaagga gacaaccttg ttttagctgc ttttggtggt
     2941 ggttttacat ggggagctat tttggtaaaa tgggcatatt aatttttttc tacctactac
     3001 taaatagtta ttatttgtaa cttataaagt gaatgatgag agatttaaac tggaaaactt
     3061 tatcctctaa atacatatat aaagacagat ggtttattgc acgtgcagat agttgtttaa
     3121 tggctaatgg aaaaacaatt gaaccatatt atgttttaga atttcctaat tggtgtaacg
     3181 taattgttgt aactaataag gaagaaattg taatggttag gcaatatagg catgctataa
     3241 ataaaactac tattgaattg ccaggtggtg taattgataa aaacgaaaac cccaaagagg
     3301 ctgctataag agaagtttta gaagaaacag gatataccat acatcaaata gagtttttgt
     3361 atgccacagc accaaaccct gctactaata ataacatggc ttatt
//
LOCUS       DAOQHP010000247         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_17993_length_3386_cov_3.304413, whole genome shotgun sequence.
ACCESSION   DAOQHP010000247 DAOQHP010000000
VERSION     DAOQHP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17993_length_3386_cov_3.304413"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLY81_08005"
     CDS             <1..262
                     /locus_tag="PLY81_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30205.1"
                     /translation="FITIVNTLNSIKHSISDYVKIITQKNINVVYREDEHSFTDGETI
                     FISGTLNDGNIDATVGLVMHESSHIVYTDFNKFALLLYLFNH"
     gene            273..1073
                     /locus_tag="PLY81_08010"
     CDS             273..1073
                     /locus_tag="PLY81_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF30206.1"
                     /translation="MNSLTFSIIQTNLFWEDKAKNLEMLEQKIASIKSKTEIVVLPEM
                     FSTGFSMQPEKYAENMDGICVNWMKTIATKYKIILTGSLMIKEKETFYNRLVWMLPNG
                     KYGFYNKRHCFAYAKENEHYTPGNNRFIASVNGWKINLQICYDLRFPVWARQASNQLT
                     PEYDILLYVANWPQRRSHAWKTLLTARAIENQCYVIGVNRIGTDGNDIYHSGDSMVIN
                     ALGEVLYQKADEEDVFTIPLQKNDLEEIRTKFPFLADADDFNLLQVPN"
     gene            complement(1118..2695)
                     /locus_tag="PLY81_08015"
     CDS             complement(1118..2695)
                     /locus_tag="PLY81_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNF30207.1"
                     /translation="MATTDQFIQSINNGYTFKGEHVKIGVGMLDGKAVSDASVNIPFK
                     TLNRHGLVAGATGTGKTKTLQMLTECFSNNSIPVLVMDIKGDFSGIAQAGSLNDKIAE
                     RYALLKETYTPAAFPVEFLTLSDEKGARLRATVSEFGPILLSKILGLNDIQSGLVAVL
                     FKYCDDNKLPLLDLKDFIKVLQYAGDEGKDELEKSYGKISTTSTGTILRKVIELQQQG
                     ADVFFGERSFEVDDLMRINNEGKGMVNVLRLMDMQNRPKLFSTFMLQLLAELYANCPE
                     EGDLDKPKLIMFIDEAHLIFQEASSALMQQIETVIKLIRSKGIGIFFCTQNPQDIPAS
                     VLAQLGLKVQHALRAFTAADRKVIKTAAENFPETTFYQTNELLTQLGIGEAFITLLNE
                     KGIPTPLVHTLLCSPKSRMDVISDAEMDAVIAESRLVKKYNEVIDSQSAYEILTEKLN
                     IAEEKTKEEQQQKEQAKEEEKQEKEEAKKEKDEPGFFDNPTVKQIGRTAASVITRSLL
                     GALGISSYTRKKRKSLW"
     gene            3018..>3386
                     /locus_tag="PLY81_08020"
     CDS             3018..>3386
                     /locus_tag="PLY81_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30208.1"
                     /translation="MKHTIVKQTLFSLISILFFVATTAFTVFAIKYDVNSVREKILKT
                     VFVGEYYSNGSTPEEIAKYYPDTTITFKKPDGVDSKAKYILQTLNYLPVYEFPMLLAD
                     IKDSELNDPNFDYEKIDDRIK"
BASE COUNT         1142 a          565 c          493 g         1186 t
ORIGIN      
        1 atttattact atagttaata cattaaattc tattaaacat tctatatcag attatgtaaa
       61 aataatcacc caaaaaaata taaatgttgt atatagagaa gacgaacatt cgtttaccga
      121 cggagaaact atattcattt ctggcacatt aaatgatgga aatatcgatg ctactgttgg
      181 tttagttatg cacgaatcat ctcatatcgt atatacagat tttaataaat ttgctctcct
      241 tctttacttg ttcaatcatt aattaattaa ttatgaatag ccttactttt tcaataatac
      301 aaaccaatct tttttgggag gataaagcaa aaaacctaga aatgcttgag caaaaaattg
      361 cttcaattaa atccaaaact gaaattgttg ttttgccaga aatgtttagt acaggcttta
      421 gcatgcagcc tgaaaagtat gcagaaaaca tggatggtat ttgtgtaaac tggatgaaaa
      481 ccattgcaac aaaatataaa attattctta caggaagttt gatgattaaa gaaaaggaga
      541 cattttataa tcgtttggtt tggatgctac ccaatggaaa gtatggtttt tacaataaac
      601 gccattgctt tgcttatgcc aaagagaatg aacattacac acctggtaat aatagattta
      661 tagcaagtgt taacggttgg aaaattaatt tacaaatttg ttacgattta agatttcctg
      721 tttgggcaag gcaagccagt aaccaactaa ctcctgagta tgatatttta ttgtatgtag
      781 ccaattggcc gcaaagaaga agccatgcat ggaaaacatt acttactgca agagcaattg
      841 aaaaccagtg ttacgttatt ggtgtaaaca gaataggtac tgatggtaac gatatttatc
      901 atagtggtga tagtatggtt ataaatgctt taggagaagt tttataccaa aaagccgatg
      961 aagaagatgt atttacgata cctctccaaa aaaatgactt ggaggagata agaacaaagt
     1021 ttcctttttt agccgatgcc gatgacttta atttactgca agtgccaaat taaatttttt
     1081 aaaccaaata ctataacaca gcctcacaca actaatttta ccataaactt tttctttttt
     1141 tgcgtgtata actgctaatg cccaatgccc ctaataagct tcttgttatt acgcttgctg
     1201 cagttctgcc aatttgtttt actgttggat tatcaaaaaa gcctggttcg tctttttctt
     1261 ttttcgcttc ttctttttct tgtttttctt cttcttttgc ttgctctttt tgttgttgtt
     1321 cttcctttgt tttttcttcg gcaatattta gtttttcagt aagtatttca taagcacttt
     1381 ggctatcaat cacttcattg tattttttca ctaatctact ttctgcaatt actgcatcca
     1441 tttctgcatc agaaataaca tccattctac ttttaggaga acagagtaag gtgtgcacca
     1501 gtggtgtagg tattcctttt tcgttgagta aagtaataaa agcttcacct attcctaatt
     1561 gtgtaagtaa ctcattagtt tggtaaaaag ttgtttcagg aaaattctct gccgcagttt
     1621 ttattacttt tctatctgct gcagtaaatg ctcttagtgc atgctgcact tttaatccta
     1681 attgtgctaa aacacttgct ggaatatctt gtgggttttg cgtacaaaag aaaatgccaa
     1741 tacccttact tctaattaat ttaataaccg tttctatttg ttgcattaat gcactacttg
     1801 cctcctgaaa aattaaatgt gcctcatcaa taaacataat tagtttaggc ttatccaaat
     1861 ctccttcctc tgggcagttt gcatataatt cagccaacag ttgcagcata aatgtagaga
     1921 aaagttttgg tctgttttgc atatccatta gccttaaaac gtttaccatt ccttttcctt
     1981 cattattaat tcgcattaaa tcatctactt caaaactcct ttctccaaaa aacacatctg
     2041 caccctgttg ttgtaattct ataacttttc taagtattgt acctgtactg gtagttgaaa
     2101 tttttccata ggatttctct aattcatctt ttccttcatc tcctgcatat tgtagaactt
     2161 taataaagtc tttcaaatct aataagggta gcttattatc atcgcaatac ttaaataata
     2221 cagcaaccaa accactttga atatcattaa ggcctaaaat tttactaagt aaaataggtc
     2281 caaattcact aactgtagcc cttaacctag cacccttttc atcacttaat gttaaaaact
     2341 ctacaggaaa agcagcaggt gtataggttt cttttaataa agcataacgc tctgcaattt
     2401 tatcatttaa actacctgct tgtgcaatac cactaaaatc tcctttaata tccattacca
     2461 aaacaggaat actattgtta ctaaaacact ccgtaagcat ttgcaaggtt ttggttttac
     2521 ctgtgcctgt tgcaccagct accaagccat gtctgtttaa ggttttaaaa ggaatattca
     2581 ctgaagcatc gcttacagct tttccatcta acattcctac accaatttta acatgctctc
     2641 ctttaaaagt gtagccattg ttaatgcttt gtataaattg atcggtagtt gccattgtaa
     2701 aaatttacag gcaagataat ggtatttttt attctgtatt catacaataa aaaatacttc
     2761 taaaaaatta atggaaaaag aaagataaaa tcaatctagt gtacaaaaag tgttgaaata
     2821 gtcattaaaa taacactaat aattactaag gcaatttctg aagtatcgtt ggtagttgta
     2881 agaaactttt tcataacaaa gaatttacat tttaatagac gacgaataga atacaaaggt
     2941 tgcaattgcc aaaaaatacc caaaaggagg tataatttaa tgattgaatt agtatatctt
     3001 taaatcaaaa tttttatatg aaacatacta ttgtaaagca aactttattt tcattaatta
     3061 gtatattatt ttttgtagca acaactgcat ttactgtatt tgcaattaaa tacgatgtaa
     3121 atagcgttag agaaaagata ctaaaaacag tttttgtagg agagtattat tcaaacggct
     3181 caacaccaga agaaattgca aaatattatc ccgatacaac cataacattt aaaaaacccg
     3241 atggtgtaga ttcaaaagca aaatatatct tacaaacttt gaactattta cctgtgtatg
     3301 aatttccaat gttattggcc gatataaaag attcagaatt aaatgatcca aattttgatt
     3361 atgaaaagat agatgacaga attaaa
//
LOCUS       DAOQHP010000248         3373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18100_length_3373_cov_4.419530, whole genome shotgun sequence.
ACCESSION   DAOQHP010000248 DAOQHP010000000
VERSION     DAOQHP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18100_length_3373_cov_4.419530"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLY81_08025"
     CDS             complement(<1..509)
                     /locus_tag="PLY81_08025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNF30209.1"
                     /translation="MFTGIIETTGVIKQIIKQNSNLTFVIETKFTNELKVDQSIAHNG
                     ICLTVEKVENNLHTVTAIEETINKTTIASWKIDDIINIERCLKVNSRIDGHFVQGHVD
                     TTAVCCEKLDINGSCTFTFSIAEKFANLIIEKGSICVNGISLTCFNVTKNSITVAIIP
                     YTYNNTNMHT"
     gene            complement(530..2563)
                     /locus_tag="PLY81_08030"
     CDS             complement(530..2563)
                     /locus_tag="PLY81_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30210.1"
                     /translation="MLLQTILKYIYKKSWLIVLIGISIAYFPLISSIMSIKNDSCVLS
                     YPIFYFFSNQLNNDIIPFWHFNMHFGFALYADPGTPFLNPIFWLFALANSSIYTYVLY
                     ILFHIVLGSIGMFLLGKQLGFEQKTNTILAVAYIAGGYFVAHLQHSNHIIECTYLPFV
                     INFLYALFKKPNLKNTLLLGLFLYLFTISGYPGFAIGMPYYIIILGIGYIINQSYKKK
                     FPEKLRAMGLFCISVAIAIILCLPFLYALYTNMENFQRASTFSGNSYLFEGGASPVLG
                     FASFILPLASVVKNSPFLSSDMAWNNMYIGIVVLIFFLASIKYKNLFIILPHLMAIAF
                     FLDISFEGQIKQLFFKLPLLNFLRYNGGLRIYAMLSMIIVAGYTINNFFITEKLDTKR
                     LVTIIKILFIIVSAVLVTTIIYSLINHSFIFNNEGSLAKKILHINLSQAIILQSVYVL
                     IVLFFFKKWINNKRSVLLIALVDIIICFWVNLPFSGLSIKSMPTITRDIEASSKFINE
                     QQSFTIQTDSVLKPAINHVVYAPALLSNKVGVIPPHAYPSGKKTYFEFIEKNGLNYFN
                     NKPTCFLQSKNTVALPYTINAESIKIECSTTQQDTLIIFQNFDKNWKAYSNNNMLTIT
                     QWKNNFMQIILPANTKNIQLIYNDNAINKLLFVPIIGLLTILFILLYTYKKNN"
     gene            2708..2905
                     /gene="rpsU"
                     /locus_tag="PLY81_08035"
     CDS             2708..2905
                     /gene="rpsU"
                     /locus_tag="PLY81_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNF30211.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKSKTLLQLRERQTFTKPSVK
                     RRGEVLKAIYKQQIASGKIEG"
     gene            2970..>3373
                     /locus_tag="PLY81_08040"
     CDS             2970..>3373
                     /locus_tag="PLY81_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNF30212.1"
                     /translation="MQVTQQNSTIVDFLNYIKFEKRYSLHTVIAYQTDLEQFFVYINA
                     QYDSPTVQSINSMMIRSWLACLKGDEKLSAKSLNRKISSLKTFFRHLLKNQVITQTPM
                     STVVAPKISKRLPVFVQENNLETLFNHVEFTN"
BASE COUNT         1300 a          462 c          464 g         1147 t
ORIGIN      
        1 gtatgcatat ttgtattgtt ataagtataa ggtataatag caactgtaat actattttta
       61 gtaacattaa aacatgtaag gcttatgcca tttacacaaa tagaaccttt ttctattatt
      121 aaattagcaa atttttctgc aatactaaaa gtaaaagtac agctaccatt aatgtctaat
      181 ttttcacagc aaactgcagt agtatcaaca tgcccttgta caaaatgtcc atcaatccta
      241 ctattaactt ttaagcatct ttcaatatta ataatgtcat caattttcca acttgcaata
      301 gtagttttat taattgtttc ttcaattgct gttactgtat gtaagttgtt ttctactttt
      361 tctacggtta agcaaatacc attgtgtgca atgctttgat caacttttaa ttcgttagta
      421 aatttggttt caataacaaa tgtgaggttg ctattttgtt taataatttg tttaattaca
      481 ccagtggttt caataattcc ggtaaacatt agtttgcaat ttgttttagt taattatttt
      541 ttttataagt ataaagtaaa ataaatagta tagttaataa gccaattata ggcacaaaaa
      601 gtaatttgtt tatagcatta tcgttataaa ttaattgaat gtttttggtg tttgcaggca
      661 gaataatttg catgaaattg tttttccatt gtgttatggt tagcatgtta ttattgctgt
      721 atgctttcca gtttttatca aagttttgaa aaattattaa agtatcttgt tgggtggtgc
      781 tacattcaat ttttatactc tctgcattaa tagtgtaagg taaagcaact gtatttttag
      841 attgtaaaaa acaagtaggt ttattattga aatagtttaa gccatttttt tctataaact
      901 caaaatatgt ttttttacca gatggataag catgaggcgg aattacccca actttattac
      961 ttaataatgc aggtgcataa acaacatggt ttatagcagg ttttaaaaca gaatcggttt
     1021 gaatagtaaa actttgttgc tcattaataa attttgaaga agcttctata tctcttgtta
     1081 tggttggcat acttttaatg cttaaaccag aaaagggtag gtttacccaa aagcaaataa
     1141 ttatatctac taaagcaata agcaatacac ttcttttgtt attgatccat tttttaaaaa
     1201 aaaataatac aattaataca taaacacttt gtaaaataat tgcctgagaa agattgatgt
     1261 gtaaaatttt ttttgctaaa cttccttcat tattaaaaat aaaagaatga ttaattaaac
     1321 tgtaaattat tgtggtaact aaaacagcac taactattat aaataatatt ttaatgatgg
     1381 taactaacct tttagtatct aatttttcag taataaaaaa attattaata gtatatcctg
     1441 caacaattat catgctaagc atagcataaa ttcttaaccc accattgtag cgtaaaaaat
     1501 ttaataatgg aagtttaaag aatagctgtt ttatttgtcc ttcaaatgaa atatctaaaa
     1561 agaaagcaat agccattaaa tgaggaagaa taataaataa gtttttgtat ttaatagatg
     1621 ctaaaaagaa tattaaaact acaataccaa tatacatatt attccaagcc atatcagacg
     1681 ataagaaagg gctatttttg acaacacttg ctaatggtaa aataaaactt gcaaaaccta
     1741 aaacagggct tgccccacct tcaaacaaat aactattacc actaaaagta gatgcccttt
     1801 gaaaattttc catattcgta tagagtgcat ataaaaaagg caggcataaa ataattgcaa
     1861 ttgcaacact tatacaaaac aaccccattg ctcgaagctt ttctggaaat ttttttttat
     1921 aagattgatt aattatatag ccaattccaa gtataataat atagtaaggc ataccaattg
     1981 caaaaccagg gtaaccacta atggtgaaca aatacaagaa taaacccaat aataaagtgt
     2041 tttttaaatt gggcttttta aataaagcat ataagaaatt aattacaaaa ggcaagtagg
     2101 tacattcaat aatatggttg gagtgttgca agtgtgcaac aaaataacca ccagcaatat
     2161 aagcaactgc aagaattgta tttgtttttt gctcaaaacc taattgtttg cctagtaaaa
     2221 acataccaat tgaacccaat acaatatgaa ataaaatata tagtacatag gtgtaaatag
     2281 aactgtttgc caacgcaaac aaccaaaata ttggatttaa gaaaggagtt ccaggatcgg
     2341 cataaagggc aaagccaaaa tgcatattaa aatgccaaaa aggaataatg tcgttattta
     2401 actggttact gaaaaagtaa aatataggat aagacaatac acagctatcg tttttaatag
     2461 acattataga gcttattaaa ggaaaataag caatacttat tcctattaaa acaataagcc
     2521 aacttttttt ataaatatat tttaatatag tttgtaataa catttttata aatacttctt
     2581 atttaaaaat gcttttaata gctgtaaaac tatactaatt gctataatac atttataaaa
     2641 tatttggtat tatgcactaa gctagtatat ttgcacccca tttcatcatt aaaggaggta
     2701 tataaatatg ctaattattg attctaaaga ttgcgaaaac atagacaaag ctctaaaaaa
     2761 gtacaagaaa aagtttgaaa aaagtaaaac tttgttacaa ttaagagaac gtcaaacttt
     2821 tactaaacct tctgtaaaac gcagaggcga ggttttaaaa gcaatctaca agcaacaaat
     2881 agctagtgga aaaatagaag ggtagtaaaa catttatacc caaacatatt taagtagctt
     2941 tagttacact aacttgagct acttttttta tgcaagtaac tcaacagaat agcactattg
     3001 tagattttct taattatata aaatttgaga aacgctattc attgcataca gtgatagcct
     3061 accaaaccga tttggaacag ttttttgtat atataaatgc ccaatacgat agtccaactg
     3121 tacaatctat taattccatg atgataagaa gctggctggc ttgtttaaaa ggagatgaaa
     3181 agttatcggc aaaatcatta aacagaaaaa tttcttcctt aaaaactttc ttcaggcatt
     3241 tactaaaaaa tcaggtaatt acgcaaacgc caatgtccac agtagtagct ccaaaaatta
     3301 gtaaaagact accggttttt gttcaagaaa ataatttaga aaccttattt aatcatgtag
     3361 agtttaccaa taa
//
LOCUS       DAOQHP010000249         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18229_length_3360_cov_3.793041, whole genome shotgun sequence.
ACCESSION   DAOQHP010000249 DAOQHP010000000
VERSION     DAOQHP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18229_length_3360_cov_3.793041"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLY81_08045"
     CDS             <1..237
                     /locus_tag="PLY81_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF30213.1"
                     /translation="INGTEGVFGLQRAFKIAGAKNLIVSLWQVPDKETAELMQLFYTN
                     LMQGNSIRNSFYKAQKEMRSKHAPFAWAAFILIE"
     gene            complement(246..1634)
                     /locus_tag="PLY81_08050"
     CDS             complement(246..1634)
                     /locus_tag="PLY81_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30214.1"
                     /translation="MALYNNIYNLSPVWLQNVLVSAYGYNWKKKRYGGIFNKEYTAAK
                     QRENFTIQQWHNYQNEQLTKILLHAYNNVPYYKNKFTKLGLTITDLKQVNIDTLDKLP
                     FLLKEDVRQFGTTILMSNSREEGGSFFASSGSTGTPIQVCYSNNMHQRWFALNEARIK
                     NWAGVNKEMSRGMIGGRRVVPDADNNPPFYRYNFFEKQVYFSAYHISPVNINNYAKAF
                     NKYSIDYMTGYAMSNYILARMFLENNIQIPPLKAVLTSSEKLTPTMRETISKAYNCKV
                     FDAWSGMEYCSLISECEHGNLHISPDAGIIELLDNNLQPVKVGEIGNVYCTGFLNFDQ
                     PLIRYKIGDQMILRNTTCGCGRSMPVIDEIIGRVEDVVYGKDGREMVRFHGIFVGLKT
                     VKLAQVIQNSLNEITIKLVPDKALADDEKELIIKRVKSQLGDIDIIIEEVMDIPKTAN
                     GKIKAVISTIKK"
     gene            complement(1707..>3360)
                     /locus_tag="PLY81_08055"
     CDS             complement(1707..>3360)
                     /locus_tag="PLY81_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNF30215.1"
                     /translation="LKVVGNDSVNLIGNVLVKNAGLLVDYTQVYYIIDSAQIKFEEDG
                     IDFGRFTVKDKFNNQALVKGKLYEKNFKNMAFDFEANTNKLLLLNTKAKDNNQFFGTA
                     IGKASLSFKGPETNAKMTIIASSNDTSHIYIPNSVSKESDEADFIVFKQFGKEMEVQK
                     KSSAFNLLVDLDLTANEKVSIDVILDDIAGDVIKATGNGRLHIKAGTNEKLDIRGRYN
                     IENGLYDFNFQSLIKKPFILMPDVGNYIEWSGDAMDAKIHVDASYETNNVRLSDLTGN
                     ASGIMSSNVSNLRESVFVIAQLRDQLMQPSIKFKIDFPQNSPVKTDPDFAQFLGRMEK
                     DENEMLQQATSLIVFGSFAPYGKGLLAGGNSYTSNISGYVNSLSQRIMSAASKIISGF
                     LAKLFNDKSLKVDLGTSIYSSGSILNQGVNTTTSKVDRQIFNFKIGKSFFNNNVIVTF
                     GSDLDFNIGNAASGDLQWLPDLNVEFVLSKDRKLRAIIFNKNSLDISGATFGRRNRQG
                     ISFSYRQDFERFFSKASNQQSPTSSKPTTSLSKDVEFIRKDP"
BASE COUNT         1069 a          592 c          447 g         1252 t
ORIGIN      
        1 attaatggta ccgagggagt ttttggtttg caacgtgcct ttaaaattgc aggtgctaaa
       61 aatttaattg taagtttatg gcaagtgccc gataaggaaa ctgctgagtt aatgcaatta
      121 ttttatacca acttaatgca aggtaatagt atcagaaatt ctttttataa agctcaaaaa
      181 gaaatgagaa gcaaacatgc accttttgct tgggctgctt ttattttaat tgaataatga
      241 aaaatttatt ttttaatggt ggaaataaca gctttaattt ttccattagc tgttttagga
      301 atatccatta cttcctcaat aattatgtca atatcaccta actggctttt tactctttta
      361 ataattaatt ccttttcatc atctgccaaa gctttatctg gcacaagttt tatagttatt
      421 tcgtttaaac tgttttgtat tacctgtgct agctttactg ttttaagtcc aacaaaaatt
      481 ccatgaaaac gaaccatttc tcttccatct tttccataca caacgtcttc tactctacca
      541 ataatttcat ctatcactgg catgcttcta ccacaaccac aagttgtatt ccttaaaatc
      601 atttggtcgc caattttgta tctaataagt ggttgatcaa aatttaaaaa acctgtacag
      661 taaacatttc ctatctctcc tacttttact ggttgtaaat tattatctaa aagttcaata
      721 atgccagcat ctggactaat atgcaaatta ccatgttcac attctgaaat taggctacaa
      781 tattccatgc cactccatgc atcaaaaact ttacagttat aagccttgct tatggtttct
      841 ctcattgtag gtgtaagctt ttcgctagag gtaagcacag cttttaatgg tggtatttga
      901 atattatttt ctaaaaacat tcttgccaaa atataattac tcatggcata gcctgtcata
      961 taatctattg aatatttatt aaaagcctta gcatagttgt ttatatttac agggctaata
     1021 tgataggcag aaaaataaac ttgtttttca aaaaagttgt atctgtaaaa aggaggatta
     1081 ttatcagcat ctggcactac ccttctacca ccaatcatac ctctactcat ttctttattt
     1141 acgcctgccc aattttttat tctagcttcg ttcaatgcaa accagcgttg gtgcatattg
     1201 tttgaataac aaacttgtat tggagttcct gtacttccac tagaagcaaa aaaactacct
     1261 ccttcttctc tactattact catcaatatt gtagtaccaa actgcctaac atcttctttc
     1321 aacaaaaaag gtaatttgtc taatgtatca atatttactt gttttaaatc tgttattgta
     1381 agccccaatt ttgtaaactt atttttataa tagggcacat tattataggc atgtaataaa
     1441 atttttgtta gctgctcatt ttgataatta tgccattgtt gaattgtaaa attttctctt
     1501 tgttttgcag cagtatattc cttattaaat attcctccat accttttctt tttccaatta
     1561 tacccataag cagaaactaa tacattttgc aaccatacag ggcttagatt gtaaatatta
     1621 ttgtataatg ccactctaaa aaatttaaaa gagaataatg tattgaaaaa tttgttctag
     1681 aacgaaataa aagtaatgaa aattgtttaa ggatcttttc ttataaattc aacatcttta
     1741 cttaatgagg ttgttggttt tgaagaggtt ggtgattgtt gattgctggc tttgctaaaa
     1801 aaacgttcaa aatcttggcg ataactaaaa ctaatacctt gcctgtttct tctgccaaaa
     1861 gtagcaccac taatatctaa actattttta ttaaaaatga tggctcttaa ttttctgtct
     1921 tttgataata caaactcaac attcaaatct ggcaaccatt gtaaatctcc tgaagctgca
     1981 ttaccaatat taaaatctaa atcgctgcca aaggttacaa ttacattatt attgaagaaa
     2041 cttttaccaa ttttaaaatt aaatatttgc ctatctacct tactggtagt tgtatttaca
     2101 ccttggttta atatactacc actactataa attgaagttc ctaaatctac ttttaaactt
     2161 ttatcattaa atagctttgc taaaaatcct gagataattt tagaagctgc cgacataatt
     2221 ctttgtgata aactatttac atacccagaa atattggaag tgtaactatt accaccagcc
     2281 aataagcctt tgccataagg tgcaaaagag ccaaatacta ttaaggaagt tgcttgttgc
     2341 aacatttcgt tttcgtcttt ttccattctg cctaagaatt gtgcaaaatc aggatcggtt
     2401 ttaacggggc tgttttgagg aaaatctatt ttaaatttaa tagaaggctg catgagttga
     2461 tctcttaatt gggctattac aaaaacgctt tctcttaaat tgcttacatt gctactcatt
     2521 atgccagaag cattgccagt aaggtcggat aaacgaacat tgtttgtttc gtaagatgca
     2581 tctacatgaa ttttagcatc cattgcatca ccactccatt caatataatt gccaacatct
     2641 ggcattaaaa taaatggctt tttaattaaa gattgaaagt tgaagtcata taaaccattt
     2701 tcaatattgt atcttcctct aatatccagt ttttcgttgg ttccagcttt aatgtgtaat
     2761 cttccgttac ctgttgcttt aattacatct ccagcaatat catctaaaat tacatcaata
     2821 gatacttttt cattggctgt taaatctaaa tctaccagca aattaaatgc agatgatttt
     2881 ttttgcactt ccatttcttt accaaactgt ttaaatacaa taaaatcagc ttcatcactt
     2941 tctttgctta cactattggg tatataaatg tgtgaagtat cgttagagct agcaataatg
     3001 gtcattttag cattggtttc tggcccttta aaacttaatg aggctttgcc aattgccgtt
     3061 ccaaaaaatt gattattatc tttagcttta gtatttaata ataatagttt attggtgttg
     3121 gcttcaaaat cgaaagccat atttttaaaa ttcttttcgt atagttttcc tttaactaat
     3181 gcctgattat taaatttatc tttaacagta aatcttccaa aatctatccc atcttcttca
     3241 aactttattt gagcagaatc tatgatgtag taaacttgcg tatagtcaac caatagccct
     3301 gcatttttta ccaacacgtt acctattaag tttacagaat cgtttccaac tactttcaat
//
LOCUS       DAOQHP010000250         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18262_length_3356_cov_3.408361, whole genome shotgun sequence.
ACCESSION   DAOQHP010000250 DAOQHP010000000
VERSION     DAOQHP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18262_length_3356_cov_3.408361"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLY81_08060"
     CDS             <1..303
                     /locus_tag="PLY81_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30216.1"
                     /translation="SSKQKHTEDYDLWLQLLSDKLVIEKINEPLLLYRVHSNSVTSSI
                     HRKRNSFFTIATTKKRFLWSRIKHGKWGIFETQILYTFCINLIMGLGKEIKNKIGF"
     gene            300..1490
                     /locus_tag="PLY81_08065"
     CDS             300..1490
                     /locus_tag="PLY81_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF30217.1"
                     /translation="MKYNTYIIKRKIESVFIYPFILLGKCIALLKPLKKEYNIFYFFP
                     FYHTGGAENVHALITQATGDSNCIIYFTKTSADTTFLKQFQQSNCDIKNISSFTDNKW
                     LYFLNLIYRGIIVTYINKQSKTPIVFNGQCNFAYKISPWVKKEIKLTELIHSFNSFSW
                     IRIPFIPYLHQTIMISKVRINDHIKQYQTIGIPQEYSNRINYISNGIELPENAPNKNY
                     NNHLNILYVGRGTIEKRVHIIAAIAEEVHKTNPEINFIFAGDVANAIPENLKQHCILK
                     GNIVDKNELANLYRQAHILLITSSTEGFPLVVMEAMANGCTIIATPVGDIPIHIQNNQ
                     NGQVVTEVDNENKIIEEMKKFIIYYQQHKYLLEENSLRNIHYAQQNFSIHSFNKAYKK
                     ILSK"
     gene            1501..2001
                     /locus_tag="PLY81_08070"
     CDS             1501..2001
                     /locus_tag="PLY81_08070"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNF30218.1"
                     /translation="MKKLILIILMIVSFQIVQAQSTFILIRHAEKDTSNKGSTTMNAN
                     PPLSQKGKERAKKLADVLNTYTINEIYSTNFIRTANTVTPLAIKNNIQIKFYNHKKLV
                     EFANQLLLEKNKTIVIAGHNTTTPALTNLLLKQNKFTALKETEYDKIFIVSINNMEAT
                     VKVIEY"
     gene            complement(2013..2606)
                     /locus_tag="PLY81_08075"
     CDS             complement(2013..2606)
                     /locus_tag="PLY81_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNF30219.1"
                     /translation="MKTDAEFYLTIAQESKAEYKDRGSRFLAFAFPVQDVEECKKQVA
                     TLKKIHTKAVHHCFAYKLGLDGNNFRANDDGEPAGSAGKPILGQINSKQLTNVLVVVV
                     RYFGGTLLGVPGLINAYKTATSLALQTTPIIQKPIEIIKDFQFDYTKMNEVMIIVKKY
                     NCTVLKQDAQLFCLLTVAIPKSSLEAVQFKINEIINR"
     gene            complement(2609..>3356)
                     /locus_tag="PLY81_08080"
     CDS             complement(2609..>3356)
                     /locus_tag="PLY81_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNF30220.1"
                     /translation="IKKVVIGCTDTFNLVNGSGINFLKENGVEVEVNILQHECETLNK
                     NFFTFHQKKRPYIILKYAQSANNKIASHKSERTFISNYFSNNLVHRWRSEEAAILVGT
                     TTALKDNPALTNRLGYGNNPVRLVIDKHLQIPTHYNLLDNSTKTIIFNFLKDDVKQNT
                     FFVKLNMHENVIHQIVQFCTKNNLISILVEGGTNLLQSFINQYLWDEARVITNNTLFI
                     EDGVDAPNFNGQILNKISLLHDTITFYTPQ"
BASE COUNT         1274 a          507 c          438 g         1137 t
ORIGIN      
        1 agcagcaagc aaaaacacac tgaagattat gatttgtggt tacagctttt atctgataaa
       61 ttagtaatag aaaaaataaa tgagccatta ctattgtata gggtacattc taattcggta
      121 acaagtagta ttcatagaaa aagaaattct ttttttacca ttgcaactac taaaaaaaga
      181 tttttatgga gtagaattaa acatggaaaa tggggaattt ttgaaacaca aattttatac
      241 actttttgca ttaatttaat aatgggctta ggtaaagaaa ttaaaaataa aattggcttt
      301 tgaaatacaa cacttatata ataaaacgaa aaatagagag tgtattcatc tatcctttca
      361 tacttttagg taagtgtatt gctctattaa aaccactaaa aaaagaatat aatatttttt
      421 attttttccc cttttatcat accggcggtg cagaaaatgt tcatgcttta attacacaag
      481 caacaggtga tagtaactgt ataatttact ttaccaaaac ctctgcagat actacttttt
      541 taaagcaatt tcaacaaagc aattgtgata taaaaaacat ctcctctttt accgataata
      601 aatggttgta ctttttaaat ttaatttatc gtggcattat tgttacttat ataaataagc
      661 aaagtaaaac tcctattgta tttaacgggc aatgcaattt tgcatacaaa atatctcctt
      721 gggttaaaaa agaaatcaag ttaacagagt taattcattc atttaacagt ttttcctgga
      781 taagaattcc atttatacct tacctgcatc aaaccatcat gataagtaaa gtaagaataa
      841 atgatcatat aaaacaatac caaacaattg gtattccaca agagtatagc aaccgaatta
      901 attatatatc caacggtatt gaacttcctg aaaacgcccc taataaaaac tataataacc
      961 atttaaacat tttgtatgtt ggcagaggca ccatagaaaa aagggtacac attattgcag
     1021 caattgcaga agaagttcat aaaactaacc cagaaataaa ttttattttt gcaggagatg
     1081 ttgccaatgc aattccagaa aacttaaagc aacattgtat tttaaaaggt aatatagttg
     1141 ataaaaatga attggcaaat ttgtatagac aagctcatat tttattaatc acttcctcta
     1201 cagaaggctt tcctttggtt gtaatggaag caatggcaaa tggttgtact attattgcaa
     1261 cacctgtagg agatatacca attcacattc aaaacaatca aaatgggcaa gtagtaacag
     1321 aagttgacaa tgaaaataag ataatagaag aaatgaaaaa attcattatt tattatcagc
     1381 agcataagta tttattagaa gaaaattcct taagaaatat tcactacgct cagcaaaatt
     1441 ttagtattca ttcatttaat aaagcgtata aaaaaatatt aagtaaataa actaaccagt
     1501 atgaaaaaac taattctaat aattttaatg attgtaagtt ttcaaatagt acaagcacaa
     1561 tctactttta tcttaatacg ccatgcagag aaagatacaa gtaataaagg ctctacaaca
     1621 atgaatgcta accctccact ttcacaaaaa ggtaaagaaa gggcaaagaa attagctgat
     1681 gttttaaaca cttatactat aaacgaaatt tattcaacaa attttattcg tacagccaac
     1741 actgtaactc cattagctat aaaaaacaat attcaaataa aattttacaa ccataaaaag
     1801 ttagttgaat ttgccaatca gcttttgctt gaaaaaaata aaactattgt aattgctggg
     1861 cataatacca caacacctgc attaactaac ctgttattaa aacaaaataa gtttactgcc
     1921 ttaaaggaaa cagagtacga taaaatattt attgttagta taaataatat ggaggctaca
     1981 gttaaggtaa tagaatatta aggaataaac ttttaccgat taataatttc attaatttta
     2041 aactgcacag cctctaagct tgatttaggt atagcaacag ttaataaaca aaacaactgt
     2101 gcatcttgct ttaaaacagt acaattgtat tttttaacaa ttatcattac ttcattcatt
     2161 tttgtataat caaactgaaa gtctttaata atttctattg gcttttgaat aattggtgtt
     2221 gtttgtaaag ccaatgatgt tgcagtttta taagcattta ttaaacctgg tacacctaat
     2281 aatgttcctc caaaatatct tacaacaaca actaaaacat tggttaattg cttactattt
     2341 atttgtccta aaataggttt acctgcagag cctgctggtt cgccatcgtc attagctcta
     2401 aaattattac catctaaacc caacttataa gcaaaacaat ggtgaaccgc tttagtatgt
     2461 atttttttta atgtagcaac ttgtttttta cattcttcta catcttgcac aggaaaagca
     2521 aatgctaaaa atctacttcc tctatcttta tattcagctt tagattcctg agcaatagtt
     2581 aaataaaatt cagcatctgt tttcatagtt attgaggagt ataaaatgta attgtatcat
     2641 gtagaaggct aatcttattc aaaatttgtc cattaaaatt aggagcatcc actccatctt
     2701 caataaataa agtattatta gtaattaccc tagcttcatc ccataagtat tgattaatga
     2761 aactttgaag caaattagtg ccaccttcta ccaaaatact tattaagtta tttttagtac
     2821 agaattgtac aatttgatgg ataacatttt catgcatatt caatttaaca aaaaaggtat
     2881 tttgctttac atcatctttt aaaaaattaa aaattatggt ttttgtgcta ttatctaata
     2941 aattatagtg ggtaggaatt tgtaagtgtt tatctattac caacctaaca ggattattgc
     3001 cataaccaag cctattggtt aatgcagggt tatcttttaa agctgtagtt gtacctacta
     3061 aaattgcagc ttcttcactc ctccaacgat gcactaaatt gttactaaag taattactga
     3121 taaaagttct ttcactttta tgactagcaa ttttattgtt ggcagattga gcatacttta
     3181 aaataatata aggacgcttt ttttgatgaa atgtaaaaaa atttttgttc aaagtttcac
     3241 actcatgctg aagaatgttt acttctactt ctactccatt ttcttttaaa aaatttatac
     3301 cagaaccatt tactaagttg aacgtatcgg tacaaccaat tacaactttt tttatt
//
LOCUS       DAOQHP010000251         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18359_length_3345_cov_3.380243, whole genome shotgun sequence.
ACCESSION   DAOQHP010000251 DAOQHP010000000
VERSION     DAOQHP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18359_length_3345_cov_3.380243"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLY81_08085"
     CDS             complement(<1..956)
                     /locus_tag="PLY81_08085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF30221.1"
                     /translation="MQFFVKLIYIKKFILLHFLVLLSSLIFAQKPTANFTTNVIKGCA
                     PLIVQFNDLSTNIPTNWSWDLGNGVTSTQQNPVTTYFTPGVYTIKLIAINASGADTVI
                     KTNFITVHDVPTVNFNADKQTGCFPLKVQYGDMSVANSGSITTWNWDFGDGTTSTIKN
                     PQHTYVVAGNFPVTLKIINSEGCTKTLTKPNYINVPGGVIANFDIGSISSCTLPVDVS
                     FNNTSTGTGILNYQWDFGNGNTSTLPNPTTNYNSNGSYSVTLITSNNQGCKDTLVKTN
                     GVIIGTVKADFSFVGTCLDYPTVFTNTSTPATGTYFWSFDDGT"
     gene            complement(1065..>3345)
                     /locus_tag="PLY81_08090"
     CDS             complement(1065..>3345)
                     /locus_tag="PLY81_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF30222.1"
                     /translation="DSIVKNNIVNIAQTIANFTTDTLVCPSTSIYFLNHSSGNNLNYQ
                     WSYGDGNTANGFAVNHSYTNQGIYTVRLIATDDIGCADTLVRTNYVKVYYPIANFTIS
                     DSTAVCPPLMVNTTNNSLYAGSTNWSFGNGSFSTNTNPSHLYIYPGNYTIKLVVKNLG
                     GCADSTTKNVIINGPTGTFSYPAVIACNPTQVKFKATSENSISNTWDFNNGVTSKTIA
                     DSTAYTYTNGGHYVPKLILEDAAGCKVPIVGKDTIKVKYVQASFAPVSKVVCDSSFIQ
                     FNDSSTTNDIITNWNWSFGDGKKSTQTNPNHFYNVNGFHSVKLRVTTQTGCTDSVYFN
                     NLIKVVSSPKLIVNGDTSVCKNGSLTFTAIQTNPDTSSVTWNWNFGNGNIYNVQNPPT
                     QTFANAGGLLMTTKITNSSGCFNKVIKNIKVHELPNVNAGADVTICRNQTISLNATGA
                     NTYTWYGNIATLNSTTIANPIAKPLINITYAVVGKSIYNCEATDSVTITVQQPLKINV
                     QKADTICLGQTAEINATGTDNYTWYPTLYIDKPNQSAVKIHPAKDTLMNYMLIGKDNN
                     QCFTDTAYVKIKTYPIPSISVEQNDITVNAGTSFNLKTKSTADVTKWKWSPNKYIDNP
                     NVASPKAVAKESIVYTCVATNEGKCLAREEVKVTVVCNGGNIFVPNTFSPNGDGMNDV
                     FYPRGKGVYTIKSFRIFNRWGEMVFEKTNFQANDASAGWDGMYKGVKQPTDAYVYSIE
                     IMCDNSISVPAKGSITILR"
BASE COUNT         1093 a          535 c          518 g         1199 t
ORIGIN      
        1 gtgccatcat caaaactcca aaaatatgta ccagttgctg gtgtggatgt gttggtgaaa
       61 acagttggat aatctaagca agttccaaca aaactaaaat ctgctttaac agttccaatt
      121 attacaccat ttgttttaac taaagtgtct ttacaacctt ggttattact agtaattaaa
      181 gtaacagaat aggaaccatt gctattatag ttagttgtag gatttggaag tgttgaagtg
      241 ttaccattac caaaatccca ctgataattt aaaattcctg ttcctgtaga tgtattatta
      301 aatgaaacat ctactggtaa agtacaacta gagatagagc caatatcaaa atttgctata
      361 accccaccag gtacattaat atagtttggt ttggttaatg tttttgtaca tccttcgcta
      421 ttaattattt ttaatgttac aggaaaattt cctgcaacaa cataagtatg ttgtgggttc
      481 tttattgttg aagtagtgcc atcgccaaaa tcccaattcc aagttgtaat acttcctgag
      541 ttggcaacac tcatatcgcc atattgaacc tttaatggaa aacagccagt ttgtttatct
      601 gcattaaaat ttacagttgg cacatcgtgt acagttataa aatttgtttt aataactgta
      661 tcggcaccac ttgcattaat ggcaattaac ttaatagtat atacaccagg agtaaagtaa
      721 gtagtaactg gattttgttg cgttgaggta acgccatttc ctaaatccca tgaccaattg
      781 gttggaatat tagttgataa atcgttaaac tgaactatta atggtgcaca acctttaata
      841 acattagtag taaaattggc tgtaggtttt tgagcaaata ttaaactact taagaggact
      901 aagaagtgta gtaaaataaa ttttttaata tatataagtt taacaaaaaa ctgcacagat
      961 aattacattt aacttttata aacatcaata ataatgagca aataaaatgc tcatttgctg
     1021 ctttaaattg ttattaaatg taaatatagt aaaaaaaata ttttttatct taatatggtt
     1081 atactgcctt ttgctggaac agagatactg ttatcgcaca ttatttctat gctgtataca
     1141 taggcatcag ttggttgttt aacaccttta tacattccat cccatcctgc acttgcatca
     1201 ttagcctgaa agtttgtttt ttcaaaaacc atttcacccc atctattaaa aattctgaaa
     1261 cttttaattg tatatacgcc ttttcctcta ggataaaaaa catcgttcat accatcgcca
     1321 tttggagaaa aagtatttgg cacaaaaata tttccaccat tacatacaac tgtaactttt
     1381 acttcttctc ttgctaagca ttttccttca ttggtagcta cacaggtata aacaatagat
     1441 tcttttgcta cagcttttgg agaggcaaca ttggggttat ctatatattt attagggctc
     1501 catttccatt tagttacatc agcagtactt ttggttttta aattaaatga tgttcctgca
     1561 tttactgtaa tatcattttg ctccaccgaa atagaaggaa ttggatatgt ttttattttt
     1621 acataagccg tatctgtaaa gcattgatta ttatccttgc caattaacat ataattcatt
     1681 aaggtatctt ttgcaggatg aatttttact gcagactgat tgggtttatc aatatacaag
     1741 gtaggatacc aagtataatt atcagtacca gttgcattaa tctctgctgt ttgaccaagg
     1801 caaattgtat ctgctttttg aacgttaatt ttaagtggtt gttgcacagt aatagttaca
     1861 gaatctgttg cttcgcaatt gtaaatactt ttaccaacaa cagcataagt aatattaatt
     1921 aatggcttag caattgggtt tgcaatagtt gttgaattaa gtgttgcaat attaccatac
     1981 caagtataag tatttgctcc tgttgcattt agtgagatag tttggtttct acaaattgta
     2041 acatctgcac cagcattcac atttggcaat tcatgaactt taatgttttt aataacttta
     2101 ttaaaacaac cactactatt ggtaatttta gttgtcatta ataaaccacc tgcattagca
     2161 aatgtttgtg ttggtggatt ttgaacatta tagatattac cattaccaaa attccaattc
     2221 catgttacag aagaagtatc tggatttgtt tgaatggctg taaaagttaa actgccattt
     2281 ttgcaaaccg aagtatctcc atttacaatc aattttggtg aacttactac ttttatcagg
     2341 ttattaaaat atacactatc tgtacaacca gtttgtgtag taactcttag ttttactgaa
     2401 tgaaaaccat taacattata aaaatggtta gggttggttt gggtagattt tttgccatct
     2461 ccaaagctcc aattccaatt tgtaattata tcgtttgtag ttgaagaatc attaaattga
     2521 ataaaagaag aatcgcatac aacttttgaa acaggtgcaa aggatgcttg tacatatttt
     2581 actttaatgg tatccttacc tacaattggc actttacaac ctgcagcatc ttctaaaatt
     2641 aattttggta cataatgccc accatttgta taagtgtaag cagtgctatc agcaatagtt
     2701 ttagaggtta caccattatt aaaatcccat gtgttagaaa tactgttttc ggatgttgct
     2761 ttaaacttta cctgagtagg gttacaagct attactgcag ggtaagaaaa agtacctgtt
     2821 ggcccattta ttattacatt ttttgttgta gaatcggcac agcctcctaa atttttaacc
     2881 actaatttaa tggtataatt tcctggataa atatagagat gagatgggtt agtatttgtt
     2941 gaaaaacttc cattaccaaa actccaattt gtgctgcctg catacaaaga attgttagta
     3001 gtatttacca ttaaaggagg acatactgct gtagaatcgc taatggtaaa atttgcaatt
     3061 gggtaatata cttttacata atttgttctt accaaagtat cggcacaacc aatatcatct
     3121 gttgcaatta atctaacagt ataaataccc tgatttgtat aactatggtt aactgcaaaa
     3181 ccatttgctg tatttccatc tccatagctc cattgatagt ttaagttatt accgctagaa
     3241 tgatttaaga aataaatact tgtacttggg caaaccaaag tatctgtagt aaaatttgca
     3301 attgtttggg caatatttac aatattattt ttcacaatac tatcc
//
LOCUS       DAOQHP010000252         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18477_length_3331_cov_3.373626, whole genome shotgun sequence.
ACCESSION   DAOQHP010000252 DAOQHP010000000
VERSION     DAOQHP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18477_length_3331_cov_3.373626"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLY81_08095"
     CDS             <1..503
                     /locus_tag="PLY81_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30223.1"
                     /translation="YGTPLKGKTLNQANGRTFYFYNGAGIVAIFNKLYAKPTNKLENV
                     TYQKIYNLCAKNYVRDFLKLMTYLNTTKKTEWNSECARLLKQIKTDKDFSHYDEYAKT
                     ANILFPSEKIRYSFESYTYDVSSTFSLGTIIRRQLDGSLKSITDCLKKIVKDYDPEAL
                     KLIKGY"
     gene            569..1600
                     /gene="rlmN"
                     /locus_tag="PLY81_08100"
     CDS             569..1600
                     /gene="rlmN"
                     /locus_tag="PLY81_08100"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNF30224.1"
                     /translation="MQNIRHLSYNDIENYFLQLGEKKFRAKQIWEWLWQKHAHSFDEM
                     TNLSKELRNTLQQQFTLPALKTDTIQHSDDGTVKSRFTTFDGYKVEGVLIPTEERKTA
                     CVSSQIGCSLTCKFCATGFMDRKRNLTYDEIYDQVVLLNQQSEKIFNQKLTNIVFMGM
                     GEPLLNYNNVLQAIERISAEDGLFMSTKRITVSTAGVAKMIKKLGDDKVRFKLALSLH
                     AANDKKRNEIMPINENNNIRSLTEALNYFYKQTGNEITFEYILFKDFNDSQQDADELI
                     KLYRQIPVDLVNIIEYNPIENTTFTKPDDATTTNFMEYLESKKVNARLRKSRGKDIDA
                     ACGQLANKG"
     gene            1642..2730
                     /gene="pdxA"
                     /locus_tag="PLY81_08105"
     CDS             1642..2730
                     /gene="pdxA"
                     /locus_tag="PLY81_08105"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNF30225.1"
                     /translation="MSNELYKPIIGFTCGDLNGIGIELIIRSLHDSRILEICTPIVFA
                     SNKSINFYRKSLADINFSYSSCKDFQRINTKQVNVFNCWEEEVQITPGELNSIGGKYA
                     LISLNKATQTLKEGNIDALVTAPIHKKNIYGDNFKYNGHTPYFKDVFEAKDVVMLMVA
                     ANMKVGLVTEHVAIADVAKHITKDSIVSKLKIINKTLQTDFGIDKPRIAVLGLNPHTG
                     DEGLIGTEEETIIKPAIKEAKQEMIVFGPYSADAFFTRGQHEKFDAVLAMYHDQGLIP
                     FKSLALGEGVNYTAGLNVIRTSPDHGTAFDIAGKAKANYASFLEAIYTAIDIARYRAT
                     YKELRKNQLNKMSAKLIVNGVDEKIEEQ"
     gene            complement(2801..>3331)
                     /locus_tag="PLY81_08110"
     CDS             complement(2801..>3331)
                     /locus_tag="PLY81_08110"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF30226.1"
                     /translation="SKLRPYFTVGIGAFKFNPQGIYEAPNGTKKWVDLRPLKLEGQGM
                     AEYGDRKEYSNIALQIPFGFGIKYFINEKLYLAAEIMQRYTFTDYVDDVSKTYIDPAL
                     FAKYLPKDKVAIAEQMMFKRALVNNRPVSEFVGKERGDSKNKDYYLSLFVKFGFRFGA
                     RDTDMSCPTGRRRSYY"
BASE COUNT         1273 a          509 c          521 g         1028 t
ORIGIN      
        1 tttatggcac acctttaaaa ggcaaaactt taaaccaggc aaacggaaga actttttatt
       61 tttataatgg tgctggtatt gttgctattt ttaataaact ttatgcaaaa ccaactaaca
      121 aattagaaaa tgttacctat caaaaaattt acaatttatg tgctaaaaat tatgtacgtg
      181 attttctaaa attaatgacc tatttaaata caactaaaaa aactgaatgg aactcggaat
      241 gtgcaagact attaaaacag ataaaaacag ataaagattt tagtcattac gatgagtacg
      301 ctaaaactgc aaatatactt ttcccatcag aaaaaataag gtatagcttt gaatcttata
      361 cttatgatgt tagttcaacc ttctccttag gaaccattat acgaagacaa ttagatggaa
      421 gtttaaagtc aattacagat tgtttaaaga aaatagtaaa agattatgac ccagaggcat
      481 taaaattgat taaagggtat taattggttg gcttatgcaa gaacaatcta ttctaaatag
      541 cttttaattt catgctatct ttgccgctat gcaaaatatc agacatttaa gctataacga
      601 tattgaaaat tattttttgc aattaggtga aaaaaaattc agagcaaaac aaatatggga
      661 atggctttgg caaaaacatg ctcatagttt tgatgaaatg acaaacctga gcaaagaact
      721 tcgtaataca cttcaacagc aatttaccct accagcttta aaaacagata ccattcagca
      781 tagtgatgat gggactgtaa aaagcaggtt tactactttt gatggatata aagtagaagg
      841 cgttttaata ccaaccgaag aaagaaaaac agcttgtgtt tcttctcaaa ttgggtgcag
      901 tttaacctgt aaattttgtg ccacaggttt tatggataga aaaagaaatc ttacatacga
      961 tgaaatttac gaccaagtag ttttactaaa tcagcaaagc gaaaaaatat ttaaccaaaa
     1021 gcttaccaat attgttttta tgggtatggg tgagcctttg cttaactaca acaatgttct
     1081 acaagcaatt gaaagaataa gtgccgaaga tggtttgttt atgagcacta aaagaataac
     1141 tgtatctaca gcaggtgttg caaaaatgat taaaaagtta ggcgatgata aagtacgttt
     1201 taaattagcc ttaagcctac atgctgcaaa cgacaaaaaa cgaaatgaga taatgcccat
     1261 taatgaaaat aacaacatta gatctttaac agaagcatta aattattttt acaaacaaac
     1321 tggtaatgaa ataacctttg aatacatttt atttaaagat tttaacgact cgcagcaaga
     1381 tgctgatgaa cttataaaac tatataggca aataccggta gatttagtaa atattattga
     1441 atataaccca atagaaaata ctacattcac caagccagat gatgcaacca caaccaattt
     1501 tatggagtat ttagaaagta aaaaagtaaa tgcccgttta agaaaaagca gaggaaaaga
     1561 tatagatgct gcctgtgggc aacttgctaa taaaggataa aacattttat aattaatttt
     1621 acaataatta atacacttat aatgagcaac gaattatata agccaataat aggatttact
     1681 tgtggcgatt taaatggaat tggtattgaa ttaattattc gttcattaca cgatagccgt
     1741 attttagaaa tatgtacacc catagttttt gctagtaata aaagtattaa cttttacaga
     1801 aaaagtttag cagatattaa tttttcatac tcaagctgta aagactttca acgcataaat
     1861 accaaacagg taaatgtttt taattgctgg gaagaagaag tgcaaataac acctggtgag
     1921 ttaaacagca ttggcggtaa atatgcttta atttcattaa acaaagccac acaaacctta
     1981 aaagaaggaa atattgatgc cttagttacg gcccctatcc ataaaaaaaa tatttatggc
     2041 gataacttta aatacaacgg tcataccccg tattttaaag atgtttttga agcaaaagat
     2101 gtggtaatgt taatggttgc agctaatatg aaagtaggct tggtaacaga acatgttgcc
     2161 attgccgatg ttgctaaaca cattactaaa gatagtattg ttagtaaact taaaataata
     2221 aataaaactt tacaaacaga ttttggaata gataaaccac gtattgccgt tttaggctta
     2281 aacccacata ctggagatga aggattaatt ggaacagaag aagaaaccat tattaagcct
     2341 gcaataaaag aagccaaaca agaaatgata gtgtttgggc catatagtgc agatgcattt
     2401 tttacaagag gccaacatga aaaatttgat gctgttttag caatgtatca cgaccaaggt
     2461 ttaatacctt ttaaatcttt agcccttggc gaaggtgtta attatacagc aggcttaaat
     2521 gtaatacgca caagccccga ccatggtact gcttttgata ttgcaggaaa agcaaaagca
     2581 aattatgcct cttttttaga agccatttat acagcaattg atattgcaag atatagagcc
     2641 acttataaag aattaagaaa aaatcaactg aataaaatga gtgctaaact aattgtaaat
     2701 ggtgttgatg aaaaaataga agagcaataa aaaaacacta tccaaattgg atagtgttta
     2761 gcatacctgt caattttcaa tataacaaac aacaagatta ttaatagtaa cttcttcttc
     2821 tacctgtagg gcaactcata tcagtatctc ttgcaccaaa cctaaatcca aatttcacaa
     2881 aaagagataa atagtaatct ttattttttg aatcgcctct ttcttttcca acaaattcac
     2941 ttactggcct attgtttact aatgcccttt taaacatcat ttgttcagca atggcaactt
     3001 tatctttagg taaatatttt gcaaataagg ctgggtcaat ataggtttta cttacatcat
     3061 ctacataatc ggtaaatgta tatcgttgca ttatttctgc tgctaaataa agtttttcat
     3121 ttataaaata tttaattcca aagccaaaag gtatttgtaa tgcaatgtta gaatactctt
     3181 ttctatctcc atattctgcc ataccttgtc cttctaattt taatggcctt aaatcaaccc
     3241 atttttttgt accattagga gcctcgtaaa taccttgtgg attaaattta aaagcaccaa
     3301 tacctacagt aaaataaggc ctcaacttgc t
//
LOCUS       DAOQHP010000253         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18718_length_3303_cov_3.584975, whole genome shotgun sequence.
ACCESSION   DAOQHP010000253 DAOQHP010000000
VERSION     DAOQHP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_3303_cov_3.584975"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /gene="alr"
                     /locus_tag="PLY81_08115"
     CDS             <1..1840
                     /gene="alr"
                     /locus_tag="PLY81_08115"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNF30227.1"
                     /translation="FKNASILIAREADFTNHYLKTINQFKPTLFTWGFSAKNEFVINS
                     LIKQNISTTLSLQYKQQEMVFEIPFVDDANIENVINCICLMLVLKYSSIIIKEKILKL
                     QPVEMRMQLKKAINNCYVVNDTYSNDLSSLKIALDYLQQQAGNQHTTVILSDILQSGI
                     NENELYQEVAALIKQKNIHKFIGIGEQLIKHKHFFENTIQQTIFYNTVDEFLIHSNLH
                     QYKEEYILLKGARVFSFERIDEWFAYKVHQTALEINLNAIVHNLKTYQNLLQAGTKVM
                     AMVKAFSYGSGSIEIARLLQYHKVDYLAVAYVDEGIELRKAGISLPIMVMNIDESGFN
                     SVIENNLEPEIYSFPIYQAFHQYLQQQGITNFPIHLKLNTGMNRLGFDAFEVTAIATL
                     LKQNNSMVVQSVFSHLTSSEDEKEDNFTLQQFQLFQQLCNNIQNNLGYSFIKHIANSA
                     AAIRHEHLQMNMIRLGIGLYGVDSSASKKIKLQTVAFLKTTIAQIRNLKKGDTVGYNR
                     KEVLQRDSKIATIRIGYADGFSRKLGNRVGSVFIKGKLAKIVGNVCMDMSMVDVTEIE
                     NVKEDDVVEIFGNNLPVQQVATWCQTISYEILTTISQRVKRIYLEE"
     gene            1853..2056
                     /locus_tag="PLY81_08120"
     CDS             1853..2056
                     /locus_tag="PLY81_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30228.1"
                     /translation="MKVYSFIIIAIILLSCLVILYKSFLIIKQGLNKEYNIKGRELIL
                     YYLLIGLTLLSVMERHFENILRD"
     gene            complement(2241..2816)
                     /locus_tag="PLY81_08125"
     CDS             complement(2241..2816)
                     /locus_tag="PLY81_08125"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNF30229.1"
                     /translation="MKRIAIFASGTGSNTAKIIHYFKDKSKGVEIALIVSNKPTAKVL
                     TIASDNNIATLIIDKEIFFRGNAYLPQLQNASIDYIVLAGFLWKIPINLIQAYPKKII
                     NIHPALLPKYGGKGMYGSYVHEAVIANKELESGITIHFVNEHYDEGEHIFKASCTVDK
                     FETVESLAAKIHALEYEHFAKVIDSVVNKLQ"
     gene            complement(2816..3100)
                     /locus_tag="PLY81_08130"
     CDS             complement(2816..3100)
                     /locus_tag="PLY81_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30230.1"
                     /translation="MFHKLKEKWNVNWLQFTLIFTTFALGGSSCAKLASYILQLFLSE
                     KDFIYWIIYLPMVTILWPICVIIISIPLFQFPFFKNYLKKMWTRIKGSTK"
BASE COUNT         1227 a          433 c          482 g         1161 t
ORIGIN      
        1 ttttaaaaat gcaagtatat taattgcaag agaagctgat tttactaatc actatttaaa
       61 aacaataaat caatttaagc ctactttatt tacttgggga ttttctgcaa aaaacgaatt
      121 tgtaattaat agcctaataa agcagaatat atctactact ctatcattgc aatataagca
      181 acaagaaatg gtttttgaaa taccatttgt agatgatgcc aatatagaaa atgtaattaa
      241 ctgcatttgt ttaatgcttg tattaaaata tagttctatc ataattaaag aaaagatttt
      301 aaaattgcaa cctgttgaaa tgaggatgca acttaaaaaa gctattaata attgctatgt
      361 tgtaaatgat acctatagca atgatttatc atccttaaaa attgcattag attacttaca
      421 acagcaagca ggcaaccagc atactactgt tatactatca gatattttac agtctggcat
      481 taatgaaaat gaattatatc aagaagttgc agcattaatt aaacaaaaaa atattcataa
      541 atttattggc attggtgagc aacttataaa acacaagcat ttttttgaaa ataccattca
      601 acaaactatt ttttataata cggtagatga atttttaatt cactccaatt tgcaccagta
      661 taaagaggaa tatattttac taaaaggtgc cagagttttt tcatttgaaa gaatagatga
      721 atggtttgcc tacaaagtac accaaactgc attagaaatt aacttgaatg caattgtaca
      781 taatttaaaa acatatcaaa atttgttaca agctggtaca aaagtaatgg ctatggtcaa
      841 agcttttagc tatggtagcg gcagtattga aatagcaagg ttactacaat accataaggt
      901 agattatttg gctgtagctt atgttgatga aggtattgaa ttacgtaaag caggtattag
      961 cttaccaatt atggtaatga atattgacga gtctggcttt aactctgtaa tagaaaataa
     1021 tttagaacca gaaatttatt cctttccaat ttatcaagcc tttcatcaat atttacaaca
     1081 acaaggcatt actaattttc ctattcattt aaagctaaat acaggcatga atcgtttagg
     1141 gtttgatgcg tttgaagtaa cagctattgc tacactgtta aaacaaaata attcaatggt
     1201 ggtgcaatct gtttttagtc atcttacctc tagcgaagat gaaaaagaag ataattttac
     1261 tttacaacaa tttcagcttt ttcaacaatt gtgcaataac attcaaaata atttaggtta
     1321 ttcttttatt aaacatattg caaacagtgc agcagctata aggcatgaac atttacaaat
     1381 gaatatgatt cgtttgggta ttggtttgta tggtgttgat agttctgcat ctaaaaaaat
     1441 taaattacaa acagttgcat ttttaaaaac tacaattgct caaataagaa atttaaaaaa
     1501 aggagataca gtagggtaca acagaaaaga agtgttacag cgagacagta aaattgcaac
     1561 cattagaatt ggttatgcag atggttttag cagaaagtta ggcaatagag taggcagtgt
     1621 ttttattaaa ggtaaacttg ctaaaattgt aggcaatgtt tgtatggata tgagtatggt
     1681 tgatgttaca gaaattgaaa atgtgaaaga agatgatgtg gttgaaattt ttggaaataa
     1741 tttacctgta caacaagtag ctacatggtg ccaaactatt agttacgaaa ttttaacaac
     1801 cattagccaa cgggttaaaa gaatttattt agaagaataa aatataacac caatgaaagt
     1861 atatagtttt attataattg caattatttt attaagttgt ttagttatac tttacaaatc
     1921 atttctaata attaaacaag gattaaataa ggaatacaat ataaaaggca gagagttaat
     1981 tttatactac ttattaattg ggttaacttt actatctgtt atggagaggc attttgaaaa
     2041 tatattaaga gattaaaact ttaatatacg ttccttcttc cttttttcta tctattatac
     2101 caagcaaata ctggattttt atttattgct tgaaggctaa ctatattaaa ttgaattatt
     2161 ttttttacgt tttttaaagt agaaataaaa gttcctaaca aggtttgtag ggcattaaaa
     2221 tattgctaca aacaagttgt ttattgtaac ttatttacaa cactatcaat aactttagca
     2281 aaatgctcgt attctaaagc atgaattttt gctgctaaac tctcaacagt ttcaaactta
     2341 tctactgtac aacttgcttt aaaaatatgc tcaccttcgt cgtagtgttc attaacaaaa
     2401 tgaatggtga taccactttc taattcttta ttagcaataa ctgcctcgtg tacgtagcta
     2461 ccatacatac ctttgcctcc atatttgggt aaaagtgctg ggtgtatatt aataattttt
     2521 ttagggtaag cttgaataag attaattgga attttccata aaaagcctgc aagaactata
     2581 taatcaatag aagcattttg tagctgaggc aaataagcat tacctctaaa aaatatttct
     2641 ttgtctataa ttaatgtggc tatattatta tcagaagcaa tggttaaaac ttttgcagta
     2701 ggcttattgc tcacaattaa agctatttct acacctttac ttttatcttt aaaataatgg
     2761 ataatttttg cagtattact gcctgttcca cttgcaaaaa tggcaatacg tttcattatt
     2821 tggtactccc ttttattcta gtccacattt tttttaaata gtttttaaag aatggaaatt
     2881 gaaaaagtgg aatactgatg attattacac aaattggcca aagtatagta accattggca
     2941 aataaataat ccaataaata aaatcttttt cggataaaaa taattgtaaa atatagcttg
     3001 ccagtttagc acatgagcta ccacccaaag caaatgttgt aaaaataagt gtaaactgta
     3061 accaatttac attccatttt tcttttagtt tatgaaacat aactgcccga agatagggat
     3121 agaaataata tgttttttat aatcaaaatc attaaaattt cctattttat ttttttcagg
     3181 cataaaatac ccaataaatg gtatcattaa ttgataaata ttttattttt ttgaaggttg
     3241 aagaatagtt aatatatatt ttatagtttt aacttattct aaaaaataca taactctatt
     3301 tta
//
LOCUS       DAOQHP010000254         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_18811_length_3294_cov_3.418339, whole genome shotgun sequence.
ACCESSION   DAOQHP010000254 DAOQHP010000000
VERSION     DAOQHP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18811_length_3294_cov_3.418339"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="PLY81_08135"
     CDS             complement(<1..2124)
                     /locus_tag="PLY81_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF30231.1"
                     /translation="MRKFASMLFVLMLFCTLAFSQNRTITGTVNGNDGKPISGASVTI
                     KGKKGGTVTNDEGHFSISAANGDRLVFSAVGYSAKEVIVNTTSASPVSITLVQEVSNL
                     TEVIVTGVATATPKAKMTVSVTKLSADKINAVNPVSLSSALTGKVAGIKTSNSGGLPG
                     QQVDIQLRADNNLNNVGSGPLIVVDGVQLSGSLADINADDVESIEVVKGAAASALYGS
                     RAGNGVIAVTTKRGKGSGVNIVVRNEVGIQKLAHTLKVATHHPYKLANDWANFKGVFT
                     KYDGVTYPNGYSGTGFHPGIAGNRTQDADHYMDNSYGIERNPQKDVFGTGTNYTNFIS
                     VSSKSEKTGIYASFENNGQQGVVNLTDGYKRQNFRFNLDQQLASWVKFTASNLFVNRT
                     IQYPGTSSSRNSLFYDVARLETDINLSEKNPVDGQKYYPRIDQWIGEITNPMYTLWKR
                     KVNDKSRRWIANYALNFKLNSWANIDVSHTMEIENYRRSTIDPKDTWKVSGGTPATYG
                     MAYTNGGLAMFSSEDNQQNTQFTVNLMHRFGDLNVAGKLSYLYENNHYESFNVGASQY
                     VINDMEGFNNFASISSAGSYISNERAQNYFAIASFDYKDRYLLDAMFRYDGSSLFGSD
                     ARWNPYYRLSGAYRLTKDVTMKGIDEFKIRLAHGTAGIRPGFDWQYEVYTLSNGVATP
                     TQKGNKLLKPSKTTETEFGVEVNFLK"
     gene            complement(2371..>3294)
                     /locus_tag="PLY81_08140"
     CDS             complement(2371..>3294)
                     /locus_tag="PLY81_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF30232.1"
                     /translation="YGYQGIMSTIDALCGRHDVIVYDAESHACIIDGLRLHPGHRYVF
                     KHNDVEDCEKQLERATALIDKQKTGGILVITEGVFGMAGDQGKLTEIVAFKNKYDFRL
                     LVDDAHGFGTLGKTGAGAGEEQGCQDGIDLYFSTFAKSMASIGAFLAGPRIIIDYIRY
                     NIRSQIFAKSLPMPIVIGNLKRLDMLRTMPELKEKLWHNATKLQKGLKERGFDIGKTD
                     SPVTPIYMKGGVEEATAMVMDLRENYGVFASIVVYPVIPKGHIIYRLIPSAAHTDEDI
                     EITLKAFSATKEKLDAGAYKVDVIPDMAEKK"
BASE COUNT         1021 a          634 c          466 g         1173 t
ORIGIN      
        1 tttcaaaaag tttacttcca caccaaattc agtttcggta gttttagatg gttttaataa
       61 tttattaccc ttttgtgttg gagttgctac tccattactt aaagtataaa cttcatattg
      121 ccagtcaaaa cctggtctaa tacctgcagt tccgtgtgct aatcttattt taaattcatc
      181 tattcctttc atagttacat cttttgttaa tctgtatgca ccagacaaac gataataagg
      241 attccatctt gcatcagaac caaataaaga agaaccatca taacggaaca tggcatctaa
      301 taaatatctg tctttataat cgaaagaagc aattgcaaaa tagttttgtg ctctttcatt
      361 gcttatataa gagcctgcac tactaattga agcaaagttg ttgaaccctt ccatatcatt
      421 aattacatat tgagaggcac caacattaaa gctttcatag tgattatttt catataaata
      481 agataatttt cctgccacat ttaaatcccc aaagcgatgc attaagttta ctgtaaattg
      541 ggtattttgt tgattatctt ctgacgaaaa cattgctaaa ccaccattgg tgtaagccat
      601 accatacgtt gcaggtgtac caccagaaac tttccaagtg tcttttggat caatagttga
      661 tctacgatag ttttcaattt ccatagtgtg gctaacatca atatttgccc aactatttaa
      721 tttaaagttt aatgcataat tggcaatcca tctacggctt ttatcattta cttttctttt
      781 ccaaagtgtg tacattgggt tggtaatctc accaatccat tggtctattc ttggataata
      841 tttttgacca tctaccggat ttttttcact taaattaata tctgtttcta aacgagccac
      901 atcataaaat aaactgttac gagaagagct tgtaccagga tattgaatgg ttctatttac
      961 aaataaatta gatgcagtga attttaccca acttgcaagc tgttggtcaa gattaaatct
     1021 aaagttttgt ctcttataac catcagttag gtttactaca ccttgttgtc cgttgttttc
     1081 aaaagatgca taaatacctg ttttttcaga tttggagctt acagaaataa agttggtata
     1141 attagttcct gtaccaaaaa catctttttg tggattgcgt tctataccat aagaattatc
     1201 catataatgg tctgcatctt gtgttctgtt gcctgcaata ccaggatgaa atcctgtacc
     1261 actatatcca ttaggataag taactccatc gtattttgta aatacacctt taaagtttgc
     1321 ccaatcgttt gctaatttat aaggatgatg ggttgctact tttaatgtat gtgcaagttt
     1381 ttgaatacct acttcgtttc ttactacaat atttacacca gatcctttac ctcttttagt
     1441 tgttactgct attacaccat tacctgctct tgaaccataa agagcagatg ctgcagcacc
     1501 ttttacaact tcaatagatt ctacgtcgtc cgcattaata tctgcaaggc ttccagataa
     1561 ctgaactcca tctacaacaa ttaacggacc agagccaaca ttatttaagt tattatctgc
     1621 acgtagttga atatctactt gctgaccagg taagcctcca gagttggaag ttttaatacc
     1681 agcaacttta cctgttaagg cagaggataa ggaaacagga ttaactgcat taattttatc
     1741 tgcagataat tttgtaacag ataccgtcat ttttgccttt ggagttgcag ttgccacacc
     1801 agttacaatt acttcagtaa ggtttgacac ttcttgaacg agtgtaatag aaacagggct
     1861 tgcacttgta gtgttaacaa taacttcttt tgcactgtac cctacagcag aaaaaactag
     1921 tctatctccg tttgctgcag aaatagaaaa atgaccttcg tcattagtta cagttcctcc
     1981 ttttttgcct ttaatggtta cacttgctcc agaaatgggt tttccgtcgt tgccatttac
     2041 agtacctgta atggttctgt tttgagagaa tgctaatgta caaaacaaca ttagcacaaa
     2101 caacatagat gcaaattttc tcatgtaatg ttagttttaa ttttaaaaaa aagataccag
     2161 agtttaattc tttaaaagtt tagattgaaa aaaatactct atcatcttta taatgattat
     2221 taaaattata attattttaa caaatagtgc aaaaaatttt taactttttt taaaaaaaat
     2281 aatcccagtt agttttttgt tactaacaaa taccaaaaaa aggaggtagt taaataacta
     2341 cccccttgat aaaaagtatt tgtaaagttt ttatttcttt tcagccatat caggtattac
     2401 atctaccttg taagcacctg catctaattt ttctttagtt gcactaaatg cttttaatgt
     2461 tatttcaata tcctcatcgg tatgtgcagc acttggaatt aatctataaa taatatgtcc
     2521 tttaggtatt acaggatata caacaatact agcaaatact ccataatttt ctcttaaatc
     2581 cataaccatt gcagttgcct cttctacacc gcctttcatg taaataggtg taacagggct
     2641 atctgtttta cctatatcaa atcctctttc ttttaaccct ttttgtagct tagttgcatt
     2701 gtgccataat ttttctttta attctggcat tgtacgcagc atatccaatc tttttaagtt
     2761 accaattaca attggcatgg gtaaactttt tgcaaaaatt tggctacgta tattgtatct
     2821 tatatagtct ataattattc ttggcccagc taaaaatgca ccaatactag ccatgctttt
     2881 tgcaaaagtg ctaaagtata aatctatacc atcctggcag ccttgttctt cgccagcacc
     2941 tgcaccagtt tttcctaatg taccaaaacc atgtgcatca tctactaata atctaaaatc
     3001 atatttattt ttaaaggcta caatttccgt tagtttaccc tggtctcctg ccattccaaa
     3061 aacaccttca gtaatcacta gaataccgcc tgttttttgt ttatcaatta aagcagttgc
     3121 tctttccaat tgtttttcac aatcctcaac atcgttatgt ttaaacacat atctgtggcc
     3181 agggtgtaaa cgcaaaccat caataataca agcatggctt tcagcatcat atacaattac
     3241 atcatgcctt ccacacaatg catcaatagt actcattatt ccttgatagc cata
//
LOCUS       DAOQHP010000255         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19355_length_3236_cov_3.712355, whole genome shotgun sequence.
ACCESSION   DAOQHP010000255 DAOQHP010000000
VERSION     DAOQHP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19355_length_3236_cov_3.712355"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..2464)
                     /locus_tag="PLY81_08145"
     CDS             complement(536..2464)
                     /locus_tag="PLY81_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30233.1"
                     /translation="MSKKKLTLIALVASMHAFAQKDTLSGSPLDEVIITTASKVEQKQ
                     NSTGKVITVITKEQLEKSSAKTVAQVLNEQVGITINGALNIAGSVQTVYMRGANAGRT
                     LILMDGIPMNDPSTISTDFDLNLFSINDIERIEICKGAQSTLYGSDAIAGVINIITIK
                     KEIDKAVNIKATTAFGNKNTSRNNVQVFGKLNNLIYTARFAQLKTDGFSSAYDSAGNK
                     NFDNDAYKGNVANAAVQYQITKKLSFKSFVQHSSYKADIDAGVFADKRNYFINNNVLN
                     TGAGLNYKSTTLHVVGNYQYSQTRRHYNDGFAAGLPVYSTNDYNGISNFFEVYASYKI
                     NKQFTVLVGSDYRFATMDGAYVSSAWGTSKYKDTSMNQSSVYASVLFNSINNQLNIEL
                     GGRLNKHSRYGNNSTFTFNPSYNINKHFRLFASVATGFKSPSIYQLFDTWSGNKDLKA
                     EKSINYETGVQYADQKFKTRVVYFNRKIDNGIDYNYISFKYFNYIKQTVSGLELEATL
                     QPVKQVTIGANYTMLSPKETTQNRITNVDTVTYNYLLRRPKNTFSLNIAWQATKQLSL
                     SVNTKYVDDRFDVGGWAKADVKLKNYFMLNAYAEYVLNNSVKFFVDAQNIGNRKFFEV
                     NGYNTMPIMANIGVTFNW"
     regulatory      complement(2569..2778)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3098..>3236
                     /gene="infA"
                     /locus_tag="PLY81_08150"
     CDS             3098..>3236
                     /gene="infA"
                     /locus_tag="PLY81_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNF30234.1"
                     /translation="MSKQQLIKQDGIILEALSNAMFRVKLENGHEILATISGKMRMHY
                     IR"
BASE COUNT         1077 a          511 c          456 g         1192 t
ORIGIN      
        1 aaagcaccag atactatagt agcttgaaaa gaacgaatag taccacccat tttatctttt
       61 aaggatatta ataatggaat tgatcctact atatctatta cagcaaaaag agtaaaagtt
      121 actgttagta gtgcatctgt ttcaatattc atttcattgc ttttttcaaa aatataactt
      181 cttaaataat atgagtgagt aaagttgact ttaattaaac attctattaa ataccttatt
      241 gttctaaatt taaaaaaaga ggaacttcag atataaagtt cctctttaaa agaattagtt
      301 taaataacta aaaaaattta ctgctgtcta tactcctgct ttaaaatatt taaacaatta
      361 ttgcataaac agtcagaaaa ctttgttttg atataactac tttcttcatt acttagttgt
      421 atagatgtgc agttacaagc cttaatatta ttgctgttac atacaaattt gcttttacat
      481 ttaaaacaaa tttttatcaa ctgctctttc attttctaaa gctaaataaa ataaattacc
      541 aattaaatgt tacaccaata tttgccatta ttggcattgt gttgtagcca tttacttcaa
      601 aaaatttcct gttgccaata ttttgggcat ctacaaaaaa tttaaccgag ttatttaaaa
      661 catattcagc ataagcattc aacataaaat agtttttcaa tttcacatct gcttttgccc
      721 aaccaccaac atcaaatctg tcatctacat attttgtatt tacagataag cttaactgct
      781 tagtagcttg ccaggcaata tttaaagaga aagtgttttt tggtcgacgt aataaataat
      841 tgtaagtaac cgtatctaca tttgtaattc tattttgtgt tgtttctttt ggagaaagca
      901 tagtataatt agcaccaata gttacttgtt ttacaggctg taaagttgct tctaattcta
      961 aaccagatac agtttgtttg atatagttga aatatttaaa actaatataa ttataatcaa
     1021 tgccattatc aatctttctg ttaaaatata ctactcttgt tttaaacttt tggtcggcat
     1081 attgtacacc tgtttcgtag ttaatagatt tttcggcttt taagtctttg ttaccactcc
     1141 atgtatcaaa caactgataa atgctaggag atttaaatcc tgttgcaacg cttgcaaata
     1201 atctgaaatg cttattgata ttgtaagatg ggttaaaagt aaaagtgcta ttgttgccat
     1261 aacgagaatg tttgtttaac cttccgccaa gctcaatatt taattgattg tttatgctat
     1321 taaataatac cgaagcataa acagaacttt ggttcatgct ggtatccttg tatttacttg
     1381 taccccatgc agatgaaaca taagctccat ccatagttgc aaaacgataa tcgctaccaa
     1441 ccaataccgt aaattgttta tttattttat aagaagcata aacttcaaaa aagttagaaa
     1501 taccattata atcattagta gagtaaacag gtaaaccagc agcaaagcca tcgttataat
     1561 gcctgcgtgt ttggctgtac tgataattac ctacaacatg tagtgtagtg cttttataat
     1621 ttaaccctgc accagtattt aaaacattgt tattaataaa ataatttctc ttatcagcaa
     1681 aaacgcctgc atcaatatct gctttatagc tactgtgttg cacaaaagat ttaaaagata
     1741 atttttttgt tatttggtat tgtactgcag cattagcaac atttcctttg taggcatcgt
     1801 tatcaaaatt tttatttcca gcactatcat aagcactcga aaatccatcg gtttttagtt
     1861 gagcaaaacg tgcagtgtag ataagattgt taagcttgcc aaaaacttgt acattatttc
     1921 tgcttgtatt tttattacca aaggcggttg tagctttaat atttactgct ttatcaattt
     1981 ctttttttat agttataatg tttataacac cagcaatagc atcgctgcca tataatgtgc
     2041 tttgtgctcc tttacaaatt tctattcttt caatatcatt aatagaaaaa agattcaggt
     2101 caaaatctgt actaatggta cttgggtcat tcatgggtat gccatccatt aaaattaaag
     2161 ttctaccagc attggcacca cgcatataaa cagtttgcac actaccagca atgtttaatg
     2221 ctccattaat ggttatacca acttgctcat ttaaaacttg tgctactgtt ttagcactac
     2281 ttttttccaa ttgttctttg gtaattactg taattacttt accagtacta ttttgtttct
     2341 gttctacttt agatgcagtt gtaataatta cttcatctaa tgggctacca cttaaggtgt
     2401 ctttttgtgc aaatgcatgc atgcttgcaa ccagtgcaat gagggttaac ttttttttgc
     2461 tcattgttaa aatgtttttt taaagtgaaa caatgagctt tcaggaaata atagtggaga
     2521 tataaaggca ataattgcca tttacattcc atgcctttcg cccgaaagcc ttgaatgaat
     2581 gtatttggca ggtcttctga cttagcagct atttcaaatt accttcccat tttttaaaac
     2641 agtggcaaac tattatttga aatatttttc tttgttaaag aaagctactt tacagctacg
     2701 gggatagttc atgcttttca catgattccc ttttaatcat ctgcctgaag cagataaacc
     2761 aaattcgccg caaatgtgca atgtttcttt agaaataaaa aacaattttt aagtgtaagc
     2821 tatcatgctg attattagct agttaagttg gagtgtattt tatccatata tatttttttt
     2881 agttaagata tagctcaaag cagagaaaaa aaagaatatt agcaaaacat ttttttagct
     2941 taatggattg gtgtattatc gttcttttca aagtgaaagg ctttcttttt aatacaatat
     3001 ttttcataaa taagatgatt ttattatagg aaaattattt ttttcaaact ttttttgaaa
     3061 ttaagtacat actcctatct ttgcagcccg aaaaaatatg tctaagcaac aactgattaa
     3121 acaagatggt atcattttag aggctttaag caatgcaatg tttagagtaa aactagaaaa
     3181 tggacacgaa attttagcta ccatttctgg aaaaatgaga atgcattata tcagaa
//
LOCUS       DAOQHP010000256         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19480_length_3224_cov_2.981382, whole genome shotgun sequence.
ACCESSION   DAOQHP010000256 DAOQHP010000000
VERSION     DAOQHP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19480_length_3224_cov_2.981382"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLY81_08155"
     CDS             <1..648
                     /locus_tag="PLY81_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNF30235.1"
                     /translation="ETIDDTVYLSKYRTKHLVTHIADSIYSIIQDGMEDVTIFGTAGR
                     LKVPGHKYCAKTGTAQNPHGNDHAVFTAFAPKENPRIAVAVVVENSGYGGTWAGPVAT
                     LLMEKYLNDTLTVESEKRVETFSKIDLIPKEIKRWYHRKDSIRVAKEKEKEKEMEQRE
                     DITPDSTTNIKNTFDPEAEPNRKENDKNNNPSTLDQPPLLLHNTEKKKKKDSIKP"
     gene            645..1988
                     /gene="rodA"
                     /locus_tag="PLY81_08160"
     CDS             645..1988
                     /gene="rodA"
                     /locus_tag="PLY81_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNF30236.1"
                     /translation="MSIQHNSMRVSQKTDTLIIWLYAILVTIGICCIFMVEYKPGMNV
                     MQNILGGTTNFSKQTLFAGVCVIVATFIMLSDSKLFTAFANLFYAFGIVLMLATFVLG
                     KNINGSKSWIPLGGGFNLQPAELCKIFVSLALAKYLSRQETDFSKLKSQIIGAAIAMV
                     PAILSIAQQETGLALVYSAFFIVMYREGLPSTILITGISVIILVVATLLLEPNTLAII
                     LTLIAAIIIFILRRQIKRQKNILTIIVTLWLVCVSIQRFAVPYFFDNVLKCYQSTRVY
                     SMVGKDYDCTKNKHASNNTSKAVRKPDDYNVRQSKIAIGSGGVIGKGYLKGTQTRGKY
                     VPEQSTDFIFTSIGEAFGFVGSFIFLLLYLLLLLRIIRLAERQRSTFSRVYAYSVVSI
                     LFFHITVNICMTIGLFPIIGIPLPLISYGGSSLLTFTILIFIMLRLDADRQMVLR"
     gene            complement(2050..2463)
                     /locus_tag="PLY81_08165"
     CDS             complement(2050..2463)
                     /locus_tag="PLY81_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30237.1"
                     /translation="MKKILAIAITLLGFAMHSNAQGKITDVPANTTNEKINSKQAKFE
                     KEMLAALTEAGLTEEEIKKVRAIQDDAYKKTGEIRKDNSLSKEQKEEKAKEINKDKNR
                     KIREVIGKEKFSKFMDIRKRQREEKSTNKEDKSAE"
     gene            complement(2492..>3224)
                     /locus_tag="PLY81_08170"
     CDS             complement(2492..>3224)
                     /locus_tag="PLY81_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF30238.1"
                     /translation="LFTMDKKRFIFESKLPQVAIEKQANSQEHPTLLLFSRLKNPEIY
                     KQLTKLYEEYYNHIDYHNHTSHSLKYLIEFNLNKRIKAVESDTPIELDPEGEGSHGGN
                     NFGLIQWSDISRESIQIPFAFDDSRIDSSKLIQIQSIIESHEKGHVVRSFEDKFFRDY
                     FAGGFDLNAKIQYQDYIWAGASYRYKDGYAAMVGINLSNSINIGYSYDLTTSRLNTVS
                     KGTHEFVLGFILGNKYGDWCPRYNF"
BASE COUNT         1065 a          486 c          513 g         1160 t
ORIGIN      
        1 gaaacaatag atgatactgt ttacctatct aagtatagaa ccaaacattt agttactcat
       61 attgcagata gcatatatag cattatacaa gatggtatgg aagatgttac catttttggt
      121 actgcaggaa ggcttaaagt tcctggtcat aagtattgtg caaaaacagg aacagctcaa
      181 aatccacatg gtaacgacca tgctgtgttt actgcttttg ctccaaaaga aaatcctaga
      241 atagcagttg cagtagtagt agaaaactca ggttatggag gtacatgggc tggcccagta
      301 gctactttat taatggagaa atatttgaat gataccttaa cagtggaaag tgaaaaaaga
      361 gttgaaactt tttcaaaaat tgatttaata ccgaaagaaa ttaaacgttg gtaccataga
      421 aaagattcta ttagagtagc aaaagaaaaa gaaaaagaaa aagagatgga gcaaagagaa
      481 gatataacac cagatagtac tacaaatatt aaaaacactt ttgatccgga agctgagcca
      541 aacagaaaag aaaatgataa aaacaataat ccatccacat tagatcagcc tcctttatta
      601 ttacacaata cagaaaaaaa gaaaaagaaa gatagtataa agccatgagt attcagcaca
      661 atagcatgag ggtttcgcaa aaaacagata ccttaataat atggctttat gccatattgg
      721 ttacaattgg tatatgttgc atttttatgg ttgaatataa acctggaatg aatgtgatgc
      781 aaaacatttt aggaggtaca accaatttta gtaaacaaac actatttgct ggggtatgtg
      841 taattgtggc aacttttatt atgctaagcg atagtaaact ttttacagca tttgcaaatt
      901 tattttatgc ttttggcatt gtattaatgc tggcaacttt tgtgttaggc aaaaacatta
      961 atggcagtaa aagttggata ccattaggtg gaggctttaa tttacaacca gccgaactat
     1021 gcaaaatatt tgtatcattg gcattggcta aatatttatc aaggcaagaa acagacttct
     1081 ctaaattaaa atctcaaatt attggagcag ctattgcaat ggtgcctgcc attctttcta
     1141 ttgcccagca agaaacaggt ttggcacttg tttactctgc tttttttatt gtaatgtata
     1201 gagaagggct cccttctacc atacttatta caggtatttc tgtaattatt cttgtggtag
     1261 caactttatt attagagcca aatactttgg caataatatt aactttaatt gctgctatta
     1321 taatttttat acttagaagg caaataaaaa ggcaaaaaaa tattctaacc attatagtta
     1381 cactttggct tgtttgtgta agtattcaac gttttgctgt gccctatttt tttgataatg
     1441 ttttaaaatg ttatcaaagt acaagagtgt atagcatggt aggtaaagat tatgattgca
     1501 ctaaaaataa acatgcttct aacaacacta gtaaagctgt aagaaaacca gatgattata
     1561 atgtaaggca aagtaaaatt gcaattggct ctggaggggt aataggcaaa ggctatttaa
     1621 aaggtactca aacacgaggt aaatatgtac ctgaacaaag tacagatttt atttttactt
     1681 ccattggtga ggcatttgga tttgttggta gttttatttt tttacttctg tacttattac
     1741 ttttattacg aattattagg ttagcagaaa gacaaaggag tacatttagc agggtttatg
     1801 cttatagcgt agtaagcatt ttgttttttc atattactgt aaatatttgt atgactattg
     1861 gtttatttcc tataattggt attccattac cattaattag ttatgggggc tcatcgttac
     1921 ttacttttac tatcttaata tttattatgc taaggttaga tgcagatagg caaatggttt
     1981 taagatagaa ataaaaaagc tgacaataag tatgtttatg tcagcttttt ttataaaatt
     2041 tttacttaat tattcagcac tcttgtcttc tttattagtt gatttctctt ctctttgtct
     2101 tttccttata tccataaatt tagaaaattt ttccttgcca attacttctc taatttttct
     2161 atttttatct ttgtttattt cttttgcttt ctcctctttc tgttctttac ttaaactatt
     2221 gtcttttcta atttctccgg tttttttgta tgcgtcatct tgtattgctc taactttttt
     2281 aatttcttct tcagttaaac cagcttctgt taatgcagca agcatttctt tttcaaactt
     2341 tgcttgtttg ctatttattt tttcatttgt agtatttgca ggtacatccg taatttttcc
     2401 ttgtgcattg ctatgcattg caaaacccaa taaggtaatt gcaattgcta atattttttt
     2461 cattgttgaa atattttaaa taaatgtaga attaaaaatt atatctaggg caccaatcgc
     2521 catatttatt tcctaaaata aaccctaaaa caaattcatg agtgccttta cttacagtat
     2581 ttaaacgaga tgttgttaaa tcgtaagaat agccaatatt aatactattg cttaaattaa
     2641 taccaaccat agcagcataa ccatctttat atctgtagga agcaccagcc caaatataat
     2701 cttggtattg aatttttgca ttcaaatcaa aacctcctgc aaaataatca cgaaaaaact
     2761 tatcctcgaa ggatctgact acatgaccct tttcgtgact ttcaatgata ctttgaattt
     2821 gtattagctt actcgaatca atacgagaat cgtcaaatgc aaaaggtatt tgaatactct
     2881 ctctactgat gtcagaccat tgaattaatc cgaaattatt acctccatga ctaccttctc
     2941 cctctggatc tagttctatt ggcgtatcgc tttcaactgc tttaatacgt ttatttaaat
     3001 taaattctat caaatatttt aaactatgtg aagtatgatt gtgataatca atatgattgt
     3061 agtactcttc ataaagctta gttagctgtt tgtatatttc aggattttta agcctactaa
     3121 agaggagtaa tgttggatgc tcttgagaat ttgcctgttt ttcaattgcc acttgaggta
     3181 gcttgctttc aaaaataaat cgttttttat ccatagtaaa taaa
//
LOCUS       DAOQHP010000257         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19482_length_3224_cov_2.636478, whole genome shotgun sequence.
ACCESSION   DAOQHP010000257 DAOQHP010000000
VERSION     DAOQHP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19482_length_3224_cov_2.636478"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLY81_08175"
     CDS             <1..243
                     /locus_tag="PLY81_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF30239.1"
                     /translation="NEYILEGNLTIRDITKPVVLNVEYNGTMVDPYGQTKAGFEITGK
                     INRKDFNLKWSATTEAGGIVVSDEVRLQLSAQMIKQ"
     gene            327..878
                     /locus_tag="PLY81_08180"
     CDS             327..878
                     /locus_tag="PLY81_08180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNF30240.1"
                     /translation="MNIEIISGSPRTESITKRIALFLDKEFKNKCDHNIGLIDLSNWN
                     LGLLQEVFKTVDTTPDKFKPLAERIFAADAFVLVTPEYNGSYSPALKNLFDHFPKQSR
                     KVFGIVTASTGALGGARSSQQMLLFVPALFGITSPYLLITPHVDKKFDENGNLLDLAF
                     QSAISTFKQEFLYLAESVHKTKN"
     gene            856..2775
                     /locus_tag="PLY81_08185"
     CDS             856..2775
                     /locus_tag="PLY81_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNF30241.1"
                     /translation="MYTKQRIKESGGVVTSPFSFQYLLMSDAIKHECGLAYIRLRKPF
                     SYYLQKYGTAMYGLNKLYLLMEKQHNRGQDGAGIAIVKLNTEAGYPYLHRIRSIAPQS
                     IAEIFATINNDLNEIEKYQPDIKQHPGLLKGHLPILGELMLGHLRYGTQGKNNVEFCH
                     PFIKRNIQPGRNLALAGNFNLVNTDELYDLLNIKPGIFQKQSDLAAMMETIHHFLIKE
                     DEQNPNNANIVALLQKAISLFDGGYTIGGLIGNGYSFILRDAHGIRPAYYFINDEFIV
                     AASERSAIRTVFNLNEKEVLELMPAHALIVDNAGNYTIHKIVEPKERRACSFERIYFS
                     RGSDEKIYRERIALGHQLSKTVLESIDYNLKQTIFSYIPNTAEVAFYGLVKGMEEYLN
                     KIKVDRILSWNGNFSQEKLEEMVNRKIRQEKIAIKDVKLRTFITEDKSRNEMVQHVYD
                     ITYGTVKDNEDTLVVIDDSIVRGTTLKQSIITMLARLRPKKIIIVSSAPQIRYPDCYG
                     IDMSKMGDFIAFKAAIELLKERGLEDIIINVQTKIVELQRNNQLHTENVVKQIYKPFT
                     PQEISNKIAALITPPALQIPIQVIFQDIENLHKSCPTNTGDWYFTGNYPTPGGNKVCN
                     KAFLNYLEGKDVRGY"
     gene            complement(2849..>3224)
                     /locus_tag="PLY81_08190"
     CDS             complement(2849..>3224)
                     /locus_tag="PLY81_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNF30242.1"
                     /translation="QKPKQFFGAARKIENGGSLTILATALIETGSKMDEVIFEEFKGT
                     GNMELQLDRRLANKRIFPAIDLVSSSTRRDDLLLQPDVLQRMNILRLYINDMNTEEAM
                     NELLKRMKGTKDNEEFLASMNK"
BASE COUNT         1209 a          476 c          529 g         1010 t
ORIGIN      
        1 aacgagtaca tattagaagg caatttaacc attagagata ttactaaacc agtagtatta
       61 aatgtagaat acaatggcac aatggttgac ccatacgggc aaacaaaagc aggatttgaa
      121 ataaccggta aaataaacag aaaagatttt aatttaaaat ggagtgctac taccgaagca
      181 ggtggaattg tagttagcga tgaagttaga ttacaattaa gtgcacaaat gataaaacaa
      241 taatcaaaat atttatctta attaataaaa gtgttttatc cgtaaacagc atctttgttt
      301 aaaattttct taaaaaacta ggtataatga atattgaaat cattagtggt agcccaagaa
      361 cagaaagtat aaccaaaagg atagcattat ttttagataa agaatttaaa aataaatgtg
      421 accataatat tggattaatt gatttaagta attggaattt aggcttatta caagaagtgt
      481 ttaaaacagt agatactaca ccagacaagt ttaaaccttt ggcagaaaga atttttgctg
      541 ccgatgcatt tgtattagta actccagaat acaatggcag ttactcacct gctttaaaaa
      601 acttatttga ccattttcct aaacaaagca gaaaagtttt tggaattgta actgctagca
      661 ctggtgccct tggtggtgcc agaagtagtc agcaaatgtt attatttgta ccagctttat
      721 ttggtataac atcgccatat ttattaataa cgccccatgt tgataaaaag tttgatgaaa
      781 atggaaatct gttagacctt gcatttcaat cagctataag tacatttaaa caagagtttt
      841 tatacttagc agaaagtgta cacaaaacaa agaattaaag aaagtggcgg agttgtaact
      901 tcgccatttt cttttcaata tttacttatg agtgatgcta taaaacatga atgcggtttg
      961 gcttatataa gattaagaaa acctttttct tattatcttc aaaaatatgg tacagcaatg
     1021 tatgggttaa acaagttgta ccttttgatg gaaaaacaac ataatcgtgg gcaagatgga
     1081 gcaggaattg ctatagttaa acttaataca gaagctggtt atccttactt acatcgtata
     1141 agaagcattg ctccacaatc tattgctgaa atttttgcta caataaataa cgacttaaat
     1201 gaaatagaaa aataccaacc agatattaaa caacaccctg gcttactaaa aggacatcta
     1261 cccattttag gagaacttat gctaggacat ttaaggtatg gtacacaagg gaaaaataat
     1321 gtagaatttt gccacccttt tattaaaaga aatattcaac cagggagaaa cttagccctt
     1381 gcaggcaatt ttaacttagt aaacaccgat gagttatatg atttattaaa cattaaacct
     1441 ggcatatttc aaaagcaaag cgatttggct gcaatgatgg aaaccattca tcacttttta
     1501 ataaaagaag atgaacaaaa ccccaataat gccaacattg tagccctttt acaaaaagct
     1561 attagcttat ttgatggtgg ctatactata ggtggtttaa ttggtaatgg gtatagtttt
     1621 atattaagag atgctcatgg cataaggcct gcttattatt ttattaatga tgaatttatt
     1681 gtagctgcaa gtgaacgctc tgccattaga actgttttta accttaacga aaaagaagtg
     1741 ttagaactaa tgccagcaca tgctttaata gtggataacg caggcaatta tactatccat
     1801 aaaattgtag agcctaaaga aagaagagct tgcagttttg aacgtattta ttttagtaga
     1861 ggaagtgatg aaaaaattta cagagaaaga attgcattag ggcatcaact tagtaaaaca
     1921 gttttagaaa gtattgatta taatttaaaa caaactattt tttcttatat acccaatact
     1981 gcagaagttg ctttttatgg tttagttaaa ggcatggaag aatatttaaa caaaataaaa
     2041 gtagatagaa ttttaagttg gaatggaaat ttttcgcaag aaaaattaga agaaatggtg
     2101 aacagaaaaa ttaggcaaga aaaaattgcc attaaagatg taaaacttag aacatttatt
     2161 accgaagata aaagccgtaa tgaaatggta caacacgtgt acgatattac ttatggcaca
     2221 gtaaaagaca atgaagatac acttgtagta atagatgata gcattgtaag aggtaccact
     2281 ttaaagcaaa gtattattac catgctggca agattaaggc ctaaaaaaat tattattgta
     2341 tcgtctgccc cacaaatacg ctatcctgat tgttatggaa ttgacatgag caaaatgggc
     2401 gactttattg cttttaaagc agcaattgaa cttttaaagg aaagaggttt agaagatata
     2461 attataaatg tgcaaacaaa aattgtagag ttacaacgaa acaaccaatt acatacagaa
     2521 aatgtggtta aacaaattta caaaccattt acaccgcagg aaatttcgaa taaaattgct
     2581 gcattaatta caccacctgc tttacaaatt cctattcagg taatatttca agatattgaa
     2641 aatttacaca aaagttgccc aaccaatact ggcgactggt attttacagg aaattatcct
     2701 acaccaggtg gtaacaaagt atgcaacaaa gctttcttaa attatttaga aggaaaagat
     2761 gtaagaggat attaattatt atataacctt atatcataaa aataggctgc aattactatt
     2821 gcagcctatt ttattattaa ttcccttttt atttattcat gcttgccaaa aactcttcat
     2881 tatcttttgt gcctttcata cgtttaagca attcattcat tgcttcttct gtattcatat
     2941 cgttaatgta taggcgtaaa atattcatac gttgtaaaac atctggttgc aataataaat
     3001 catctcttct ggtagaggaa gaaactaaat caatagcagg aaatattctt ttatttgcca
     3061 atctcctatc taattgcaat tccatattac ctgttccttt aaactcctca aaaataacct
     3121 catccatttt actgccagtt tctattaatg cagttgctaa aattgttaaa gagccgccat
     3181 tttcaatttt acgggctgca ccaaaaaact gttttggttt ttgc
//
LOCUS       DAOQHP010000258         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19503_length_3222_cov_3.496685, whole genome shotgun sequence.
ACCESSION   DAOQHP010000258 DAOQHP010000000
VERSION     DAOQHP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19503_length_3222_cov_3.496685"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLY81_08195"
     CDS             complement(<1..601)
                     /locus_tag="PLY81_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF30243.1"
                     /translation="MQTNYEKIIIVGQGLCGTFLSMYLHLAGKEIVVYNLPKKNSSSI
                     VTSGVINPVTGRRIVRTWMIEEIMPFAVNTYQKVEQLLQTSIIKQCNILDFHPTPQMK
                     IAFDDRINEEPFLQKPSNQNQWNEYLNYHFGFGEINPCWQIDIQNLLQSWREYLLTNR
                     LLKEEYFSEEQINLHKNNLVIYCDGADNTTNSYFKLLPYA"
     gene            complement(622..1218)
                     /locus_tag="PLY81_08200"
     CDS             complement(622..1218)
                     /locus_tag="PLY81_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNF30244.1"
                     /translation="MNYLAHAYLSFNKEDILIGNMISDFIKGKRKFDYPLQIQKGIQL
                     HRNIDSFTDNHFATKEAKQIFKPVVGLYAGAFIDVVYDHFLAIDKTEFETESHLNNFT
                     QTTYKSLMHNYSLMPPIFQQMLLKMKAQNWLFNYQFLWGIEKSFEGVVHRAKYLQSST
                     LVYNLFIQNYSSLQNYYSLFFKDVKAFSFNEFSSLISK"
     gene            complement(1226..2161)
                     /locus_tag="PLY81_08205"
     CDS             complement(1226..2161)
                     /locus_tag="PLY81_08205"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF30245.1"
                     /translation="MKEFLVIVLLIICTSFKSLAQTNEKDISIKINNGVLDGTLTTTN
                     NHAVLAIIIAGSGPTDRDGNNIAGVSANTYKMLAAELQKNNIATIRYDKRGVGKSLQK
                     NSSEDSLRFEDYINDAITFYHYAIDSLGYKKIFFIGHSEGSLIAIVAAQQVNVMGVVS
                     ISGAGRPIDEVILSQLESKEEVVYQQARDIFTILKNGKTIHDVPKYLYALFRPSVQPY
                     MISWLKYNPADEIKKIKPPVLIINGTCDIQTKPSEAQILYMANTKNKIVLIKKMTHTL
                     KNASDDCEDIGMKTYTDKTLPINEELIINISKFIN"
     gene            2451..2897
                     /locus_tag="PLY81_08210"
     CDS             2451..2897
                     /locus_tag="PLY81_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30246.1"
                     /translation="MKKLLFIMLICTAFSFKLKAADDLKVSNIIIENFQQSFPSVENV
                     EWKISKDVVKASFNNNNEFVTAYYFFDGSYLGYSKPVALDKLPENAIRNFTKNYPSPL
                     YNLTEVIEVTSPNGEINYYLSLLESKNGKRRILRVSEMGGVYSFND"
     gene            complement(2963..>3222)
                     /locus_tag="PLY81_08215"
     CDS             complement(2963..>3222)
                     /locus_tag="PLY81_08215"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNF30247.1"
                     /translation="TAKIRNRTNHLKGVKIAIMGCIVNGPGEMADADFGYVGSGPGKI
                     TLYKGKEVVKRNIDSNIAVDELINLLKENNAWIEPELNKEN"
BASE COUNT         1037 a          470 c          415 g         1300 t
ORIGIN      
        1 atgcataagg taataattta aagtaagaat tggttgtgtt atcagctcca tcgcaataaa
       61 taactaaatt atttttatgc aaattaattt gctcttcaga aaaatattct tcttttaata
      121 gcctatttgt aagtaaatat tctctccaac tttgtaaaag attttgaata tctatttgcc
      181 agcaagggtt aatttctcca aaaccaaaat gatagtttaa gtattcgttc cattgatttt
      241 gattggatgg tttttgtaaa aatggctcct catttattct atcatcaaaa gcaattttca
      301 tttggggtgt aggatgaaag tctaaaatat tacattgctt aataattgaa gtttgtaata
      361 attgctccac tttttgatag gtatttactg caaaaggcat aatttcttca atcatccatg
      421 ttcttacaat tcttcttcct gttactgggt ttattactcc actagttaca atacttgagg
      481 agtttttttt gggaagatta tatactacta tttctttacc agccaggtgt aaatacatag
      541 ataaaaatgt tccacataaa ccttgtccta ctataattat cttttcgtaa ttagtttgca
      601 tattttattt ttcttaaaaa tttatttact tattaaactt gaaaattcat taaatgaaaa
      661 tgcttttaca tctttaaaaa ataaggaata ataattttgc aaactcgagt agttttgaat
      721 aaataaatta tacaccaaag tagaactttg caaatatttt gcacgatgta ctacaccttc
      781 aaaacttttt tcaatacccc ataaaaattg gtagttaaac aaccagtttt gtgctttcat
      841 ttttaataac atttgctgaa aaattggtgg cattaaacta tagttatgca ttaaactttt
      901 ataggtagtt tgagtaaaat tattcaaatg actttctgtt tcaaattcgg ttttatctat
      961 tgctaaaaaa tgatcgtaca ctacatctat aaatgcacca gcatataagc caacaactgg
     1021 cttgaaaatt tgtttggctt ctttagttgc aaaatggtta tctgtaaaac tatctatatt
     1081 acgatgcaat tgaatacctt tttgtatttg caatgggtaa tcaaattttc tcttaccttt
     1141 tataaaatcg ctaatcatat taccaattaa tatatcttct ttattaaaag ataagtatgc
     1201 atgtgctaaa tagttcaatt aattattagt ttataaattt tgaaatgttt attatcaatt
     1261 cttcattaat tggtagcgtt ttatctgtat aagttttcat tccaatatct tcacaatcat
     1321 ctgatgcgtt ttttaaagtg tgtgtcattt tttttattag cacaattttg tttttggtat
     1381 ttgccatata caatatttgt gcttctgatg gttttgtttg tatatcgcat gtgccattga
     1441 taattaatac aggaggttta atttttttta tttcatcggc agggttatat tttaaccagg
     1501 aaatcatata tggttgtacc gatggccgaa acaatgcata taaatatttt ggtacatcat
     1561 gtatggtttt accatttttt aaaatagtaa atatatctct ggcttgttga tatactactt
     1621 cttcttttga ttctaattgg cttaatatta cttcatcaat aggcctacct gcaccactaa
     1681 tagatacaac acccattaca ttaacttgtt gtgcagctac tattgcaatt aaagagcctt
     1741 cgctatggcc aatgaagaaa atttttttat atcctaagct gtcaatagcg taatgataaa
     1801 atgttattgc atcattaata taatcttcaa accttaaact atcttcacta ctattttttt
     1861 gcagactttt accaacacct cttttatcgt atctaattgt tgctatgtta tttttttgta
     1921 gttctgctgc taacatttta taagtatttg ccgatactcc tgcaatgttg tttccatctc
     1981 tatctgtagg gccagaacct gcaattataa tggccaatac tgcatggttg tttgttgttg
     2041 ttaaagtacc atccagtact ccattattta ttttaataga aatgtctttt tcatttgttt
     2101 gtgccaaact tttaaaggag gtgcatatta ttagcaacac tattactaaa aattctttca
     2161 ttccgttata aatattttaa taaaagtagg ttattaaaac caaaattata atttaaccaa
     2221 accataacat taattttaat ttttttaaga aatgtgttga aactgctttc tgtattgagt
     2281 tttgttgaat taatgaaaat ataaattcgt tattggatta ccgttcatca tgtgtttttt
     2341 accattggtt ggttttatgt ataactactt aatcttgcta ttaaaccaat ttatttttac
     2401 atcatcaaat aaattgaaaa ctaattaaat catctttaaa actaaataaa atgaaaaagt
     2461 tactatttat aatgcttatt tgcaccgcat tctcttttaa attaaaagca gcagatgacc
     2521 ttaaagtttc taatattata attgaaaatt ttcaacagtc atttccatct gtagaaaatg
     2581 tagaatggaa aattagtaaa gatgttgtaa aagcaagttt taacaacaat aatgaatttg
     2641 ttactgccta ttattttttt gatggtagtt atttaggtta ttcaaaacct gttgcattag
     2701 ataaattgcc agaaaatgct attaggaatt ttactaaaaa ttatccttct cctttataca
     2761 acttaacaga ggtaatagaa gtaacaagcc caaatggtga aataaattat tacttatctt
     2821 tattagaatc taaaaatggt aagagaagaa ttttaagagt atctgaaatg gggggtgttt
     2881 actcatttaa tgattaatct ttcatcaact tattttattg aagttgcaac attggatatt
     2941 tttcaatgtt gtttttttat aattaatttt ctttatttag ttctggctct atccaagcat
     3001 tattttcttt taataaatta attaattcat ctactgcaat attgctgtca atatttcttt
     3061 ttaccacttc tttccctttg tacaaagtaa tttttccagg tccgctgcca acataaccaa
     3121 aatctgcatc agccatttca cctggtccat taactatgca gcccatgatt gcaattttta
     3181 ctccttttaa atggttggtt ctatttctaa tttttgcagt tg
//
LOCUS       DAOQHP010000259         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19617_length_3212_cov_3.336712, whole genome shotgun sequence.
ACCESSION   DAOQHP010000259 DAOQHP010000000
VERSION     DAOQHP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19617_length_3212_cov_3.336712"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLY81_08220"
     CDS             complement(<1..744)
                     /locus_tag="PLY81_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30248.1"
                     /translation="MSHTFHIPVLGTAYSIETPIKVAHLGISSVISIVDDILVERLRK
                     FYSQKVNLNYQPITKKDIAFRSKRITAYLNLVHKMVEAKVESMKQECFEKKGELVKYF
                     ELLPEHSALKKMYIKFIAEENSLTKQKLEQELKQNIVQGAIDVNIMSKVDKLNLNKEG
                     EVINSDALESFKGFADSTLNAAVVLSAGMNPKLYNYIENFSDFFPDENNQLRKRIILK
                     VSDFRSALIQAKFLAKKGLWVSEFRIESGL"
     gene            complement(831..2705)
                     /gene="asnB"
                     /locus_tag="PLY81_08225"
     CDS             complement(831..2705)
                     /gene="asnB"
                     /locus_tag="PLY81_08225"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNF30249.1"
                     /translation="MCAIAGIMSFKNNLVELSNLQKMANIMQHRGNDGEGFWLNNSST
                     IGFAHKRLSIIDLSKNAAQPMHYLQHEATNKYTLIFNGEIYNYVELKKDLITKGYQFF
                     TQSDTEVLLALYDCYQENCLQYLDGMFAFALWNNATQQLWIARDRFGEKPLYYNCSDE
                     QFLFASEMKALWAVGVDKTINNARLLNYLSVGMVSNPSNAEETFYNSIKKLPAAHYLI
                     INYNTKEISTKRYWQANLHEEKNLLKEEEVVEKFSYLFAQSIKRRLRSDVAVSTSLSG
                     GLDSSSVIATITALNNKNNNEKPISFSAIFPGFAKDESAFINEVVNYFNLKNYTINPT
                     AEGFVADFEKLLFHQEEPFQSSSIYTQFKVYELAKLHNIKVVLDGQGADEILAGYTKY
                     YFWYWQSLLSNFKFSTLKKEKIAAKKNNQQIDWNSKNIAAAFFPNLAARKLTSNIKNK
                     ILFNPYIEKDFINANFNEQSVQKPTIKNLNQILQFNTCTNGLEDLLRYADRNAMAHGI
                     EVRLPFLNHQLVEFVLNLPENYKIREGFTKYVLRTAMSNQLPQNIVWRKDKIGFEPPQ
                     KLWMENKKMEELLMQAKFNLAKAGILKKQVIDLKLTNTNAHEVNNYNWWFLCMGNLIG
                     "
     gene            complement(2768..>3212)
                     /locus_tag="PLY81_08230"
     CDS             complement(2768..>3212)
                     /locus_tag="PLY81_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNF30250.1"
                     /translation="LATFIPLIISSGGNSGSQASTLIIQAMAVGEIKIADWWRVLRRE
                     ILSGLMLGTVLGMIGFIRIMIWHEFFPNIYGDHFILVGFVIFFSLIGVVLWGTLSGSM
                     LPLVLKKMGADPAVSSAPFVATLVDVTGLIIYFSFAYLFLQGLIL"
BASE COUNT         1103 a          549 c          406 g         1154 t
ORIGIN      
        1 caatcctgat tcaatccgga attctgaaac ccataatcct tttttagcaa gaaatttagc
       61 ctgaattaat gcagaacgaa aatcacttac tttaagaata attcttttgc gaagttgatt
      121 attttcatca gggaaaaagt cagaaaaatt ttctatataa ttatagagtt tagggttcat
      181 acctgcagat aaaactactg cagcatttaa agtgctatct gcaaagcctt tgaaggattc
      241 taacgcatcg ctattgataa cttcaccttc tttattcaaa ttaagtttat ctactttaga
      301 cattatattt acgtctatag caccttgtac aatattttgt ttaagctcct gctctaattt
      361 ttgttttgtt aagctatttt cttctgcaat aaattttata tacatttttt ttaaagcaga
      421 atgctctggt aaaagctcaa aatattttac taattctcct tttttctcaa aacattcctg
      481 cttcatactt tctactttag cttcaaccat tttatgtact aaatttaagt aagcagttat
      541 tcttttagaa cgaaatgcaa tatctttttt agtgattggt tggtagttta aattaacctt
      601 ttgagaataa aattttctca atctttccac caaaatatca tctacaatag atataacaga
      661 tgatatgcct aaatgtgcaa cttttatagg tgtttcaata ctgtaagctg tacctaaaac
      721 aggtatatga aaagtatgtg acataataaa cagaaaatat tagggctgca aagaacctat
      781 aaataagttg ctaaataagt gactgaaatc atacccataa atgataaata ttagccaatc
      841 aaatttccca tacacaaaaa ccaccaattg taattattga cttcatgggc attggtatta
      901 gttagcttta aatcaattac ttgttttttt aatatgcctg cttttgctaa attaaatttt
      961 gcttgcatca ataattcttc cattttttta ttctccatcc ataacttttg aggtggctca
     1021 aatccaattt tatcctttct ccaaacaata ttttgtggta attgattgct cattgcagtt
     1081 cgtaatacat atttagtaaa cccttctctt attttataat tttcgggtaa gtttaaaaca
     1141 aactcaacta attgatggtt taaaaaaggc aaccttactt caataccatg agccattgca
     1201 tttctatcag cgtatctcaa caaatcttct aacccattgg tacaagtatt aaactgtaaa
     1261 atttgattta agtttttaat ggtaggcttt tgtacacttt gctcgttgaa gttagcattt
     1321 ataaaatctt tttcaatata aggattaaat aagatcttat ttttaatgtt tgaagttaat
     1381 tttcttgctg ccaaatttgg gaaaaaagct gctgcaatat tcttactatt ccaatcaatt
     1441 tgctgattat tttttttggc agcaatcttt tcttttttta aggtactaaa tttaaaattg
     1501 ctaagtaacg attgccaata ccaaaaataa tatttggtat aacctgctaa aatttcatct
     1561 gctccttgcc catctaatac aactttaata ttatgcaact ttgctaattc atatacttta
     1621 aattgagtat aaatactact actctgaaaa ggttcctctt gatgaaaaag taatttttca
     1681 aaatctgcta caaaaccttc tgctgtgggg ttaattgtat aatttttaag attaaaataa
     1741 ttaactacct cgttaataaa agcagattca tctttggcaa aaccaggaaa aatagcagaa
     1801 aaactaattg gcttttcatt attattttta ttatttaatg cagtaatagt tgctataaca
     1861 gaggagctat ctaaaccacc acttaaactg gtagatacag caacatcact tcttaacctt
     1921 cttttaatag attgtgcaaa caggtaagag aatttctcta caacttcttc ttcctttaat
     1981 agattttttt cttcatgtaa atttgcctgc caatatcttt ttgtagaaat ttcttttgta
     2041 ttgtaattaa ttattaaata atgtgctgct ggtagttttt taatggagtt ataaaaagtt
     2101 tcctctgcat ttgatggatt gctaaccata ccaacagaaa ggtagtttag caaccttgca
     2161 ttattgattg ttttatctac accgacagcc cataaagctt tcatttcgct tgcaaataaa
     2221 aattgctcat cgctacaatt gtaatacaaa ggtttttcgc caaatctatc tcttgcaatc
     2281 cataattgtt gtgtagcatt attccataaa gcaaatgcaa acattccatc taaatattgt
     2341 aaacaatttt cttggtaaca atcgtataaa gccaataaaa cttcagtatc gctctgggta
     2401 aaaaattgat acccttttgt aataagatcc ttttttagtt caacgtaatt ataaatttct
     2461 ccattaaaaa ttaaagtgta tttattggta gcttcatgct gtaaatagtg cattggctgt
     2521 gctgcatttt tgcttaaatc tattatagat aaccgtttgt gggcaaaacc aattgtagaa
     2581 gaattattaa gccaaaaacc ttccccatca tttccacgat gctgcataat attggccatt
     2641 ttttgcaaat tgcttaattc aaccaaatta tttttaaaag acataatgcc tgcaatggca
     2701 cacatgaagg ttacatttta agttacaagg atacgaataa taaatgttaa ctattaaaga
     2761 aaaaacttta taaaattagg ccttgtaaaa acaagtaagc aaaactaaaa taaattatta
     2821 aacctgtaac atccaccaaa gtagccacaa atggtgctga tgaaactgca gggtcggcac
     2881 ccattttttt taaaaccaat ggtaacatac taccacttaa tgtgccccat aatacaacac
     2941 caattaaact aaaaaatatt acaaagccta ctaaaataaa atggtcgcca taaatatttg
     3001 gaaaaaattc gtgccaaatc attattctta taaaacctat cattcctaaa acagtaccaa
     3061 gcattaaacc acttaaaatt tctcttctta aaacacgcca ccaatctgca attttaattt
     3121 cgccaactgc cattgcttga atgattaatg tagaagcctg tgagccacta ttaccaccac
     3181 tactaataat taacggaatg aatgttgcta aa
//
LOCUS       DAOQHP010000260         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19677_length_3205_cov_3.853968, whole genome shotgun sequence.
ACCESSION   DAOQHP010000260 DAOQHP010000000
VERSION     DAOQHP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19677_length_3205_cov_3.853968"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLY81_08235"
     CDS             complement(<1..653)
                     /locus_tag="PLY81_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30251.1"
                     /translation="MKALFIVGGIFFMITQLNSQTVWEKTKEKAKEKTEQRRETRTDE
                     AIDKGLDKVEKGIKGIFKKKKKKNESENKETKETANDNETTTPTTSSDNTDTTPVTPI
                     NKGFKAYSKFDFIPGEKITSYEDFSQDAVGDFPAKWSTNASGEVVTFKGVENKWLQFG
                     NNGIFFPEFVNTLPENFTIEFDMAVTDDFSEMQSGLKLYFTPVSNRNLMFDQFFDNKP
                     "
     gene            762..1286
                     /locus_tag="PLY81_08240"
     CDS             762..1286
                     /locus_tag="PLY81_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF30252.1"
                     /translation="MNTIAQLKLGNEATVKDVYNCNKQGFISFAGKYDVSEYEATDIY
                     QDAVIALIENARKGKLDNLKSSLTTYLFAIGKFMLFAYLKKQNKQVMMSIDEAVEWNS
                     YETEQQEEQTALLQKHFEQLGEQCRKMLTLFYYEEKKLEEIMHLMNYDNKDVVKSQKH
                     RCLKQLKNLMGKNG"
     gene            1279..1995
                     /locus_tag="PLY81_08245"
     CDS             1279..1995
                     /locus_tag="PLY81_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30253.1"
                     /translation="MDKQTLITQYFENNLSKEALQQFNELLQTDKAFAEEVTFQQQLK
                     AAIHLNKRQELKQQLQQYEQGKVGKINVRKWLYIAAAIIFIAGSGILVLVQKPDAKEL
                     YAAYYQPYPNTVQPIVRGNNDNEKNVFAAFNAYEKGQYETALTLFDELYTIHKKDYPI
                     LYKAICFLQLNQPNESIEILKQYNFTEYEFIVMGKWYLAMAYLKTGDKENAIPLVKYV
                     SENENPFQDAAKTLFKKLNK"
     gene            complement(1976..>3205)
                     /locus_tag="PLY81_08250"
     CDS             complement(1976..>3205)
                     /locus_tag="PLY81_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF30254.1"
                     /translation="NQQIAVLDKEISKTDQFFHTFQSEEFAIKNIQKKLHKNQLIIKY
                     TVADNKVYGLAVTYNNIYLHEIGSIAHLQNQVNLFHAALKNISPIYKKNAETLFTLLV
                     KPFYEELKNTTDIIIIPEDFLSFLPFEALINPNTNHLLITDYTISYSYALQLWQLQNA
                     ANKHITGTVAFAPDYPNLPLPVKNAITTRGDLYNLENTSKEAAQIVQINKGVLYNGNN
                     ATKNNFLNSLGKYGIYHLAMHAQIDNNRYENSNLIFSNDEKLHFYQLYALNFPAQMVV
                     LSACNTGIGTMQHGEGLMSLSRALVYSGVQSSVYSLWEVPDKETAELMILFYKNLGKG
                     QSKDEALKNAKQQFIKNNPAKSHPYYWAGFVINGNTQPIKNTNYTIWLILVGGVIILL
                     SGWWIKKKYKKTTYSVS"
BASE COUNT         1063 a          451 c          550 g         1141 t
ORIGIN      
        1 ggtttgttgt caaaaaactg gtcaaacatt aagttgcggt tgcttacagg tgtaaaatac
       61 aatttcagtc cagattgcat ttcactgaaa tcatctgtta cagccatatc aaattctatg
      121 gtaaaatttt cgggtagtgt atttacaaat tcggggaaaa aaataccatt gttgccaaat
      181 tgtagccact tgttttctac acctttaaaa gttactactt cgcctgatgc attggtactc
      241 cattttgcgg gaaaatcgcc aacggcatct tgcgaaaaat cttcataaga tgttattttt
      301 tcaccgggta taaaatcaaa tttgctgtaa gctttaaagc ctttgtttat aggcgttaca
      361 ggagtagtat ctgtattatc tgaagaagtg gtaggtgtag tagtttcatt atcattcgct
      421 gtttcttttg tttctttatt ttctgattcg tttttcttct ttttcttttt aaaaattcct
      481 tttatgcctt tttctacttt atctaatcct ttgtcaatag cttcatcggt tctggtttct
      541 ctcctttgtt ccgttttttc tttggctttt tctttggttt tttcccatac ggtttgggag
      601 tttaactgtg taatcataaa aaaaatgccc cctacaataa ataatgcttt catatttagt
      661 ttttttagct gtataaatac ttagtgtgct aaaacagaaa aaagtaaaca ttatttttta
      721 tttttttctt tgcgtttact tttactacat tatttacact aatgaataca atagcacagc
      781 taaaactggg gaatgaagca acagtaaagg atgtttataa ttgtaataag caaggattta
      841 tttcttttgc aggtaagtat gatgtttctg aatatgaagc aacagatata tatcaagatg
      901 cagtaattgc attaattgaa aatgctcgaa aaggaaaatt ggataattta aaaagttctt
      961 taactacata tttatttgcc ataggaaaat ttatgttgtt tgcttatctg aaaaagcaaa
     1021 acaaacaagt aatgatgagt atagatgaag ctgttgaatg gaacagttat gaaacagaac
     1081 agcaagagga acaaacggct ttgctgcaaa aacattttga acaattagga gaacaatgta
     1141 gaaaaatgct cacgttgttc tattatgaag aaaaaaaatt ggaagaaata atgcacctga
     1201 tgaattatga taataaagat gtagtaaaaa gccaaaaaca tagatgcctt aagcaattaa
     1261 aaaacctgat gggaaaaaat ggataagcaa acattgataa cacaatattt tgaaaataat
     1321 ttgagcaagg aagcattgca gcagtttaat gaattgctgc aaacggataa agcatttgca
     1381 gaagaagtaa cttttcaaca acagctaaaa gcagctatac atttaaacaa aaggcaggaa
     1441 ctgaaacaac aattacaaca atatgagcaa ggaaaagtag ggaaaatcaa tgtaagaaaa
     1501 tggctgtaca ttgctgctgc cattatattt attgcaggtt ctggaatatt ggttcttgtg
     1561 caaaagcctg atgcaaaaga gttgtatgct gcatattatc aaccctatcc aaatactgtg
     1621 caaccaattg taagaggcaa taatgataat gaaaaaaatg tgtttgctgc ttttaatgct
     1681 tatgaaaaag gacaatatga aacggcttta actttatttg atgaattata cacaatacac
     1741 aaaaaagact atcctattct ttataaagcc atttgttttt tgcaacttaa tcagccaaat
     1801 gaatctatag aaattttaaa gcaatacaat tttaccgaat atgaatttat agtaatgggt
     1861 aaatggtatt tggcaatggc ttatttaaaa acaggtgata aagaaaacgc tattcctttg
     1921 gtaaaatatg tatcggaaaa tgaaaaccct tttcaagatg cagcaaagac tttatttaag
     1981 aaactgaata agtagttttc ttatattttt tctttatcca ccaaccgctt aataaaatta
     2041 ttactccacc aacaagtata agccatattg tgtagttagt attttttatg ggttgtgtat
     2101 tgccgtttat tacaaatcct gcccaataat agggatgcga ttttgcagga ttgtttttaa
     2161 taaattgttg tttggcattt tttaatgctt cgtcttttga ttgaccttta ccaagatttt
     2221 tataaaataa aatcattagt tctgctgttt ctttatcagg cacttcccat aaagaataaa
     2281 cagaagattg cacaccacta tatactaatg ctcttgataa actcattaat ccttcgccat
     2341 gttgcatagt gccaatacct gtattgcagg cacttaaaac taccatttga gcaggaaaat
     2401 tcaatgcata tagttgataa aaatgtagct tttcatcatt actgaaaata aggttggagt
     2461 tttcgtaacg attgttgtct atttgggcgt gcatggctaa atgataaatg ccatatttgc
     2521 caagactgtt tagaaagttg ttttttgtag cattgttgcc attgtacaat acacctttgt
     2581 taatttgtac tatttgtgct gcttctttgc tggtattttc taaattgtat aaatcgccac
     2641 gtgtggtaat agcattttta acgggtaaag gtaaatttgg ataatctggt gcaaaagcta
     2701 ctgtgcctgt aatgtgtttg ttggctgcat tttggagttg ccataattgt aaagcataag
     2761 aataactgat agtgtagtct gtaattaata aatgattggt attaggatta atcaatgctt
     2821 caaagggaag aaaacttaaa aaatcttctg gaattataat tatgtcagtt gtgttcttta
     2881 attcttcgta aaagggcttt actaataatg taaataatgt ttcagcattt tttttatata
     2941 tagggctgat gttttttaaa gctgcatgaa acaaattgac ctgattttgt aagtgagcaa
     3001 tactgcctat ttcgtgcaaa taaatattat tgtaagtaac agctaaacca tatactttat
     3061 tatctgccac agtatatttt ataatgagtt ggtttttgtg cagctttttt tgaatgtttt
     3121 tgatggcaaa ctcttccgat tggaatgtgt gaaaaaattg gtctgttttg ctaatttctt
     3181 tgtctaatac tgcaatttgt tggtt
//
LOCUS       DAOQHP010000261         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19737_length_3199_cov_3.749364, whole genome shotgun sequence.
ACCESSION   DAOQHP010000261 DAOQHP010000000
VERSION     DAOQHP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19737_length_3199_cov_3.749364"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLY81_08255"
     CDS             <1..400
                     /locus_tag="PLY81_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF30255.1"
                     /translation="SQALGIASGAYELALKYAQERKAFGKEIAKHQAIQFKLADMATR
                     IEAARLLCLKAAWEKDNGMDYALSSSMAKVFASETAMWVTVEAVQIHGGYGYVKEYHV
                     ERLMRDAKITQIYEGTSEVQRIVIGRSILK"
     gene            466..1512
                     /locus_tag="PLY81_08260"
     CDS             466..1512
                     /locus_tag="PLY81_08260"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNF30256.1"
                     /translation="MANKPVKALSTIAATEPLKVVNIERRTLTDKDIEIEILYCGICH
                     SDLHVAQNDWGGSHYPLVPGHEIVGKVIGVGNSTTKFKINDIVGVGCIVDSCQHCTYC
                     QQGHEQFCEEGWTVVFNSKDKKHGGFTYGGFSTNIIVNEDYVVHIPAGLNLPNAAPIL
                     CAGITVYSPLKHWKVDANKTVGIIGMGGLGHMAIKIAKAMGAYVTVFTTTPTKANDAL
                     QLGADKVIISTNEQEMKHCTKQDIILNTVSAKHNINEYINLLNVDGTIIMVGIPAQPL
                     EIGAYNVIKGRKSFSGSNIGGIAETQEVLNFCAKHNITADIELINPDYINTAFERLSK
                     GDVKYRFVLDMQKI"
     gene            1522..1941
                     /locus_tag="PLY81_08265"
     CDS             1522..1941
                     /locus_tag="PLY81_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30257.1"
                     /translation="MFKYFLFILLLGFYTRTKAQKIEAIYFNLYTDSLKKEVHNYINI
                     EGKLANGKFIPLDSSHIKLSCNTGNWYGNNLVIDSSYLKDSVVITAQLKQNLHLQKQI
                     TVFIKKINEVANLKTEEEVLQSLQPTNNKNKKRKNKR"
     gene            complement(1945..2328)
                     /locus_tag="PLY81_08270"
     CDS             complement(1945..2328)
                     /locus_tag="PLY81_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNF30258.1"
                     /translation="MILPVLFISESDNKGKGVFTAQIIPSKTTIEISPVIVLNEKERA
                     IVEQTKLYNNIFEWGENNMKAAIGLGFISMYNHNYLANCVYEMDFENETMSVVTVRQI
                     EAGEELFINYNASFNDTKKVWFDAV"
     gene            2390..>3199
                     /locus_tag="PLY81_08275"
     CDS             2390..>3199
                     /locus_tag="PLY81_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF30259.1"
                     /translation="MSIEIEQINLQEQFLEIIATQDKILIKEFLDNQNISDVADLIYH
                     NTNDEATIVANMAINRAAKVFKILDFATQKQIINKLPSNKTAELLNELPADDRTDFFE
                     ELPKTVIRDLIKLLDADERKITLSLLGYPEDSVGRLMTPDYVFVYTHNTIEEVLETIR
                     KYAKNSETIDVIYIVNEQDELIDDIRIRDVILASPEKKMEELIDGRYVALHVNDDQEH
                     ASQIFKMNNRVALPVIDDNNKLLGIVTIDDMLWVANEEFSEDMQKMGGTEAL"
BASE COUNT         1234 a          438 c          539 g          988 t
ORIGIN      
        1 atctcaggca ttaggtattg caagtggtgc ttatgagctt gctttaaaat atgcacaaga
       61 acgtaaagct tttggtaaag aaattgccaa acaccaagct atacaattta agttagcaga
      121 tatggctaca cgtattgaag ctgctcgttt attatgctta aaagcagcat gggaaaaaga
      181 taatggaatg gattatgcat taagcagcag tatggctaaa gtttttgcaa gcgaaactgc
      241 tatgtgggtt actgtagagg ctgttcaaat tcatgggggt tatggttatg taaaagaata
      301 ccatgtagaa cgcttaatga gagatgcaaa aattacccag atttatgaag gcaccagtga
      361 agtgcaacga atagtaatag gtagaagtat tttaaaataa cttaccaaaa ctcttcaaaa
      421 aaatgaagag ttttttattt ttatagtaat taaaatgaat aaataatggc aaataaaccg
      481 gtaaaggcat taagcactat tgcagctaca gagcctttaa aagtggtaaa tattgaaaga
      541 agaactttaa cagataaaga tattgaaatt gaaattttat attgtggcat ttgtcattca
      601 gatttacatg ttgcacaaaa tgattggggt ggctctcatt atccattagt accaggacac
      661 gaaatagtag gtaaagtaat tggcgtaggt aatagcacta caaaatttaa aattaatgat
      721 atagtaggtg ttggatgtat tgtagatagt tgccaacatt gtacttattg ccaacaaggc
      781 catgagcaat tttgcgaaga aggttggaca gtagttttta attcaaaaga taaaaaacat
      841 ggaggcttta cttatggtgg tttttcaacc aatataattg tgaatgaaga ttatgtagta
      901 cacatacctg ctggcttaaa tttacccaat gcagcaccta tactttgtgc aggcataact
      961 gtttattcac ctttaaaaca ttggaaagtt gatgctaata aaactgtagg aattattgga
     1021 atgggaggct tagggcacat ggccataaaa atagcaaaag caatgggggc ttatgttaca
     1081 gtatttacca ctacaccaac aaaagccaac gatgcgttac aacttggtgc agataaagta
     1141 atcatttcaa ccaatgaaca agaaatgaaa cattgcacca aacaagatat tattttaaat
     1201 actgtttctg caaaacataa tataaacgag tatattaatt tactaaatgt agatggtact
     1261 attattatgg ttggcatacc tgcacaacct ttagagattg gtgcttataa tgttattaaa
     1321 ggcagaaaaa gtttttcggg ttctaatatt ggaggcattg ccgaaacaca agaagtattg
     1381 aacttttgtg caaaacataa tattacagcc gatattgaat tgataaatcc tgattatata
     1441 aatactgctt ttgaaaggct aagtaaaggc gatgtaaagt acaggtttgt gttagatatg
     1501 caaaaaatat aagttcttgc tatgtttaaa tactttttat ttatactgtt gttagggttt
     1561 tatactagaa ctaaagcaca aaagatagaa gctatttatt ttaatttata taccgatagc
     1621 ttaaaaaaag aagtacataa ttacattaat atagaaggaa aattggctaa cggaaaattt
     1681 attcctctcg attctagtca cattaaatta tcatgcaata ctggtaattg gtatggaaat
     1741 aatttagtaa tagatagtag ttacttaaaa gactctgttg taattacagc acaacttaaa
     1801 caaaacctac accttcaaaa acaaataaca gtttttataa aaaaaataaa tgaggtggct
     1861 aaccttaaaa cagaagaaga ggtattgcaa agccttcaac caaccaacaa caaaaataaa
     1921 aagagaaaaa ataaacgata gttgttaaac agcatcaaac catacttttt tagtatcatt
     1981 aaagcttgca ttataattaa taaaaagttc ttcaccagct tcaatttgcc taactgtaac
     2041 cacactcatt gtttcatttt caaaatccat ttcatataca caattagcta agtagttatg
     2101 gttgtacata gatataaaac ccaatccaat agcagctttc atattgtttt ctccccattc
     2161 aaaaatatta ttatacaatt tagtttgttc cactattgcc ctttcttttt catttaaaac
     2221 aattactgga gaaatttcta ttgttgtttt acttggaata atttgtgcag taaatacacc
     2281 tttacctttg ttatcacttt ctgaaataaa caaaacagga agtatcatat ttaaaataaa
     2341 ttataacgaa agtaatgtat aaaattaaac aacattgtaa attgcactca tgagtattga
     2401 aatagaacaa attaatttac aagaacagtt tctagaaatc attgctacac aagataaaat
     2461 tttaataaaa gagttcttag acaatcaaaa tattagcgat gtagccgatt taatttatca
     2521 taatactaac gatgaggcta ccattgttgc caatatggca attaatagag cagccaaagt
     2581 atttaaaatt cttgattttg caacccaaaa acaaatcatt aataaattac caagtaataa
     2641 aactgcagaa ttattaaatg aattacctgc tgatgataga acagattttt ttgaagagtt
     2701 acctaaaaca gttatcagag atttaattaa attgttagat gctgatgaaa gaaaaattac
     2761 cttatcctta ttaggatatc cagaagatag tgtaggcagg ttaatgacac ctgactatgt
     2821 ttttgtatat actcataata ccatagaaga agtacttgaa accattagaa aatatgcaaa
     2881 aaatagcgaa actattgatg taatttatat tgtaaacgaa caagacgaac taattgatga
     2941 tataagaatt agagatgtaa ttttagcatc gccagagaaa aaaatggaag aattaattga
     3001 tggcagatac gtagcacttc atgtaaacga tgatcaggaa catgccagcc aaatttttaa
     3061 aatgaataat agggtagcat tgcctgttat tgatgataac aataagctat taggtattgt
     3121 aaccattgat gatatgcttt gggtagcaaa tgaagaattt agtgaagaca tgcaaaaaat
     3181 gggaggtacc gaagcatta
//
LOCUS       DAOQHP010000262         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19788_length_3194_cov_3.414145, whole genome shotgun sequence.
ACCESSION   DAOQHP010000262 DAOQHP010000000
VERSION     DAOQHP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19788_length_3194_cov_3.414145"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLY81_08280"
     CDS             <1..308
                     /locus_tag="PLY81_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF30260.1"
                     /translation="QGISFKLADMATEIMASDLLIWQSCHLKETGQKLTKQSAMAKYY
                     ASEVAVKAATEAIQIFGGYGYTKDFPVEKHYRDAKLCTIGEGTSEIQKLVIAREVLQ"
     gene            397..1425
                     /locus_tag="PLY81_08285"
     CDS             397..1425
                     /locus_tag="PLY81_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30261.1"
                     /translation="MKYQIGDEIIVLLTQEEGRVIDIISEDMVMIEVKGVRFPAYMNQ
                     IDFPYFYRFSKKKSTQETNKVTKIYIDNIPKEKPQPNKFKVEDGVWLSLIPIFTLDEF
                     NDEVVEKLKIYIINKTNVAYKFQYTLKLASHDNFALENEIIAHHDFYIHDLDFANVND
                     YPNFYIDFSLSKEEKLKAEHFETCIKLKPKQIFKRIEALKETNESNISYKLFEEYPDR
                     YFEENTILLDKLKDKGFRVYEASKVKENLQPARSVIDLHIEKLSDSWKHLSNFEILTL
                     QLNEFEKWYNIAIANKLPSFIVIHGVGKGKLKDEIHEILKSKKEVKNYINQYDARFGY
                     GATEIFFK"
     gene            1440..1789
                     /gene="rnpB"
                     /locus_tag="PLY81_08290"
     ncRNA           1440..1789
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLY81_08290"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1877..2113)
                     /locus_tag="PLY81_08295"
     CDS             complement(1877..2113)
                     /locus_tag="PLY81_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30262.1"
                     /translation="MQKFKQEELIQYLYGESSADLTFEINEALKIDWELQEEINVLQR
                     TFKQLSNLKLASPRKSSVNAVLRYAKISEPVIDL"
     gene            complement(2212..2499)
                     /locus_tag="PLY81_08300"
     CDS             complement(2212..2499)
                     /locus_tag="PLY81_08300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNF30263.1"
                     /translation="MKQLPSYITNKYFLNILFFIAWMLFFDQKDFFSTIEKRRELRTL
                     IDKNKYYFKEIEVSKKQLADFQSNSIAIEKFAREKYMLKKDNEDIYIIDEK"
     gene            complement(2583..>3194)
                     /gene="eno"
                     /locus_tag="PLY81_08305"
     CDS             complement(2583..>3194)
                     /gene="eno"
                     /locus_tag="PLY81_08305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585660.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF30264.1"
                     /translation="INAAGYKAGSQIVIAMDAANSELWDAKKKKYVFHKSNGKILSSD
                     DLVKFWETWVKKYPIVSIEDGMAEDDWKGWAALTQAIGNKCQLVGDDLFVTNVERLKQ
                     GIDKNIANGLLVKVNQIGTLTETINAVTMAQYAGYNTIMSHRSGETEDVTIADLAVAL
                     NCGQIKTGSASRTDRIAKYNQLIRIEEALAHTAIYPKNLKFGK"
BASE COUNT         1176 a          462 c          470 g         1086 t
ORIGIN      
        1 ttcaaggtat tagttttaaa ttggcagata tggctacaga aattatggca agcgatttat
       61 taatttggca aagttgccat ttaaaagaga caggtcaaaa actaaccaaa caaagtgcaa
      121 tggcaaaata ttatgccagc gaagtagcag taaaagcagc tacagaagcc atacaaattt
      181 ttggaggcta cggctataca aaagattttc ctgtagaaaa acattataga gatgcaaaac
      241 tttgtaccat aggagaagga acttctgaga tacaaaaatt agttattgca agagaagtat
      301 tgcaataata ttataaataa tgcaactttc taaagccgat attttcggct ttttttattt
      361 ttatttttga caactttaaa acaaccagaa tataaaatga aataccaaat aggtgatgaa
      421 ataattgttt tactcaccca ggaagaagga agagtaatag atattataag tgaagatatg
      481 gtgatgatag aagtaaaagg tgttcggttt cctgcataca tgaatcaaat agattttcct
      541 tacttctatc gttttagcaa aaaaaaatca acccaagaaa caaataaggt tactaaaatt
      601 tatatagata atataccaaa agaaaaacca caacccaaca aatttaaagt tgaagatggt
      661 gtgtggttaa gtttaattcc aatttttact ttagacgaat ttaatgatga ggttgtggag
      721 aaactaaaaa tttacatcat caacaaaacc aatgttgctt ataaattcca atacacttta
      781 aaacttgcta gtcatgataa ctttgcttta gaaaacgaga taattgccca tcacgatttt
      841 tatatacatg atttagattt tgccaatgta aacgattatc caaattttta tattgacttt
      901 agcctaagca aagaagagaa gttaaaagca gagcattttg aaacctgtat aaagctaaaa
      961 cccaaacaaa tttttaaaag aatagaagct ttaaaagaaa caaacgaatc taatatctcc
     1021 tataaacttt ttgaagaata ccctgatagg tattttgaag aaaataccat tttattggat
     1081 aaactaaaag ataaaggttt tagggtttat gaagcaagta aagtaaaaga aaatttacag
     1141 cctgccagaa gtgtaataga tttacatatt gaaaaactta gcgacagttg gaagcattta
     1201 agcaactttg aaattttaac cctacaatta aatgagtttg aaaaatggta caatattgcc
     1261 attgccaata aactgccttc ttttattgta attcatggtg ttggtaaagg aaaactgaaa
     1321 gatgaaattc atgaaatatt aaaatcaaag aaagaggtaa aaaattatat aaaccagtat
     1381 gatgctagat ttggttatgg tgctacagaa atatttttta aatagtacat ttgcaaaagc
     1441 tataatctcg aactatcgtg ttaaataaaa ttttaataag gaaagtccgg acaacttaga
     1501 gcaattcacc agttaatagc tggttatgtt ttaaacataa agaaagtgcc acagaaaata
     1561 actaccatat ttttatatgg aaaaggtgaa aatgtaaggt aagagcttac aatttattgt
     1621 agtaatataa taaatgggta aaccttgaat gttgcaatgc cacgtataga aacgtttgag
     1681 agctgctcgt tcgagtttca gggtaggcag caaagattgt aacagtaatg ttgcaacaag
     1741 ataaatgata gtttagaaac agaatccggc ttatgaggat tatagcaata aaaaacaggc
     1801 ttaagcctgt tttttattta aactaaatac aacaaaaact agttgtataa ttattttttt
     1861 caataaatca accaaattaa aggtctatta ctggttcgct tattttagca taacgtaaaa
     1921 cagcgttaac acttgactta cgtggagaag ctaattttag attagataac tgtttaaaag
     1981 ttctttgtaa aacattaatt tcttcttgca attcccagtc tatctttaat gcttcattta
     2041 tttcaaatgt taaatctgct gaagattcgc catacaaata ttgtatcagc tcttcctgtt
     2101 taaatttttg cataataatt taagtagtat gtagttaaaa atctattaaa gaaaacaaca
     2161 ctatataaac gcatctcttt aataaatatt gttatagggc tggttaaaaa actatttttc
     2221 atcaattata tatatatcct cattgtcctt tttgagcata tacttttctc tagcaaattt
     2281 ctcaattgct attgaattag attgaaaatc agcaagttgc tttttagaaa cttctatttc
     2341 tttgaaataa tatttatttt tatcaattaa tgttcttaat tctctcctct tttctatggt
     2401 ggagaaaaaa tctttttggt caaagaaaag catccaagct ataaaaaata agatatttaa
     2461 aaagtattta ttggtaatat aagaaggtag ctgcttcata aaaaagtttg atgatgtaaa
     2521 tataactatg tattaattat actacaatca tcaaactatt aacagatgtt tattaattag
     2581 tattatttac caaattttaa atttttggga tatatagctg tgtgagcaag ggcttcttca
     2641 atacgtatta attggttgta ttttgcaatt ctatctgttc tggatgcaga acctgtttta
     2701 atttgaccac agtttaatgc aactgccaag tctgcaatag ttacatcttc tgtttcgcca
     2761 cttctatgac tcataattgt attataacct gcatattggg ccatcgttac tgcattaatg
     2821 gtttctgtta aagttcctat ttggtttact tttaccaaca atccattggc aatattttta
     2881 tctatccctt gttttaaacg ttctacatta gtaacaaata aatcatctcc tactaattgg
     2941 catttattac caattgcttg agttaatgct gcccatcctt tccaatcatc ttccgccata
     3001 ccatcttcta tagaaacaat tggatatttt tttacccatg tttcccaaaa tttcactaaa
     3061 tcatcgctac ttaatatttt gccattgctt ttatgaaaaa catatttttt ctttttagca
     3121 tcccataact cgctgttagc agcatccatt gcaattacaa tttgagaacc tgctttatat
     3181 cctgcagcat taat
//
LOCUS       DAOQHP010000263         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19879_length_3185_cov_3.245048, whole genome shotgun sequence.
ACCESSION   DAOQHP010000263 DAOQHP010000000
VERSION     DAOQHP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19879_length_3185_cov_3.245048"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLY81_08310"
     CDS             complement(<1..567)
                     /locus_tag="PLY81_08310"
                     /inference="COORDINATES: protein motif:HMM:NF036509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNF30265.1"
                     /translation="MKKNNKRQHHTTKETHLPTNTLKGILEVTNSGIGYVIMPEGVGD
                     ILVRQDNFNTAFHGDTVRVKVIKENFKSGKREGKITEVVQRKQTEFIGKLQLSNAFAF
                     FIPDSNKPMPDFFIPKDKIKTATNNEKVIVRLVKWEKGDKKPVGEVMSILTSADENDA
                     AMKEIIAAAGFALSFTDEVITETAQLHET"
     gene            complement(554..1645)
                     /gene="lpxK"
                     /locus_tag="PLY81_08315"
     CDS             complement(554..1645)
                     /gene="lpxK"
                     /locus_tag="PLY81_08315"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF30266.1"
                     /translation="MNFNTLFLKSFRVLLLPFAIIYGIVVSIRNWLFNKNYFKSAKFN
                     FPLICVGNLAVGGTGKSPMVEYLVKILQPHFKTATLSRGYKRKTKGYALANTQTTALE
                     IGDEPMQFHLKFPDVAVAVGEERLVAIPQLLHDKPDLEVIVLDDAFQHRSVNAGLNII
                     LTEYSNLYTQDFFLPTGDLRDEKRSAVRADIIVVTKCPTSITQDKKYKILRSIKPLKH
                     QRVFFTTIDYGTPYHIYNNQDEWVLTPNEEVLLVCGIANPKPLKEYLHTAVHTYYQKD
                     YSDHHIFNIDDLNEIKQVFDKIKANRKLILTTEKDAVRLMKFSEVLKDLPLYVLPVKH
                     KFLFDDTQVFHNRIIDFVNNFRLELDEEK"
     gene            complement(1635..2198)
                     /locus_tag="PLY81_08320"
     CDS             complement(1635..2198)
                     /locus_tag="PLY81_08320"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNF30267.1"
                     /translation="MTKTELRKIYKEKRKAISSKDKLKYDDLILLQLQKLYFENVQVL
                     LTYWPMELEPNMQIVTSYIKHTNYALRVAYPVTNFSNNTMCAIGVEDDTSFTTNTFGL
                     TEPKSGEEINANEIDIVFVPLFIFDLRGYRVGYGKGFYDKFLASCKNDVIKIGFCYFE
                     PIEKIDDTNNFDIPLNYCITPHNIYEF"
     gene            2546..>3185
                     /locus_tag="PLY81_08325"
     CDS             2546..>3185
                     /locus_tag="PLY81_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNF30268.1"
                     /translation="MQKEISLKITPSEAASNTTITEIIAKTVGVSPNEVFGFYKLKQS
                     IDARSRKQIWVNITVNAFINEKFIERNIEKINFQDVSNSNKKVIIVGAGPAGIFAALK
                     LIELGIKPIVVERGKDVRSRRRDLAILNKEGKINTESNYCFGEGGAGTYSDGKLYTRS
                     NKRGSIDRILNLFVQFGAEEKILYQAHPHIGTNKLPSIITAMREQIINCGGEV"
BASE COUNT         1099 a          480 c          445 g         1161 t
ORIGIN      
        1 ggtttcatgc aattgagcgg tttctgttat aacttcatca gtaaaagata atgcaaaacc
       61 tgcagcagca ataatttctt tcatagcagc atcgttttca tctgcagatg ttaaaatact
      121 catcacttct ccaacaggtt ttttatctcc tttttcccat tttactaagc gtacaattac
      181 tttttcgtta ttggttgctg ttttaatttt atcttttgga ataaaaaaat ctggcattgg
      241 tttattgcta tctggaataa aaaaagcaaa tgcattagat agctgtagtt taccaataaa
      301 ttcagtttgt tttcgttgta caacttcagt tatttttcct tctcttttac ctgatttaaa
      361 attttctttg attactttta ctcttacagt atctccatga aatgcagtat taaaattatc
      421 ttgtcttacc aaaatatctc ctaccccttc tggcataatt acataaccaa taccactatt
      481 agttacttct aaaatgccct ttaaagtgtt agtaggcaaa tgtgtttcct tagtagtatg
      541 gtgttggcgt ttattatttt tcttcatcta attctaatct aaaattattt acaaaatcaa
      601 ttattctgtt atggaatacc tgtgtatcat caaataaaaa tttatgttta actggcaata
      661 cataaagtgg caaatcttta agtacttccg aaaatttcat tagtcttaca gcatcttttt
      721 cggtagttaa aattagtttt ctattggctt ttattttatc aaaaacttgt tttatctcgt
      781 tcaaatcatc aatattgaaa atatggtggt cgctataatc tttttggtag tatgtatgta
      841 cagcagtatg taaatattct tttagtggtt ttgggttagc aattccgcac accaataaca
      901 cctcttcatt tggtgttaaa acccattcat cttgattatt gtaaatatgg tatggagtgc
      961 cataatcaat agttgtaaaa aatactcttt gatgtttaag tggtttaata cttcgtaaaa
     1021 ttttatactt tttatcttgt gtaatagatg ttgggcattt agtaaccaca atgatatctg
     1081 ctcttactgc acttcttttt tcatctctca aatctccagt tggaagaaaa aaatcttgtg
     1141 tatataaatt gctatattca gtaagtataa tatttagccc tgcatttaca gatctatgct
     1201 gaaaagcatc atctaaaact attacttcta aatcgggttt atcatgtaac aactgcggta
     1261 tagcaaccaa tctttcttct cccactgcaa cagcaacatc tggaaatttt aaatgaaatt
     1321 gcataggttc atcaccaatt tctaaagcag tagtttgagt atttgccaaa gcatagcctt
     1381 ttgtttttct tttataacct ctgctaagcg ttgcagtttt aaaatgtggt tgtaatattt
     1441 ttactaaata ctcaaccatt ggcgattttc ctgtgccacc aactgccaag tttccaacac
     1501 aaattaatgg aaaattaaat tttgcacttt taaaatagtt tttattaaat aaccaatttc
     1561 ttatgctaac tactatacca taaattatgg caaatggtaa taataaaacc ctaaatgatt
     1621 ttaaaaataa agtattaaaa ttcataaata ttgtgcggtg taatacaata atttaaaggt
     1681 atatcaaaat tattggtatc atcaattttt tctataggtt caaaataaca aaacccaatt
     1741 ttaataacat catttttaca cgatgctaag aatttatcgt aaaagccttt tccgtagcca
     1801 actctataac ctcttaaatc aaaaataaat agtggcacaa aaacaatatc aatttcattt
     1861 gcattaattt cctcgccact tttcggttct gttaaaccaa aagtattggt agtaaaactt
     1921 gtatcatcct ctacaccaat tgcacacata gtattgttgg aaaaatttgt aacaggataa
     1981 gcaactctta aggcataatt tgtgtgcttt atgtacgagg ttactatttg catatttggc
     2041 tctaactcca taggccaata cgtaagcaaa acttgtacat tctcaaagta aagtttttgt
     2101 aattgaagta aaattaaatc gtcgtacttt aatttatctt ttgaagaaat agcttttctt
     2161 ttctccttat aaatttttct taattctgtt ttagtcataa caatatatgt aaagatatct
     2221 tttactaagc agagttttgg agaagcttat tttttttcat aaagtatttt taatgcttca
     2281 acagtaatag tttctctttg ccctactgga aaatttattt atcaaattat caacagttcc
     2341 ttataacata ataatttatt gctttggagg gagtattaac aatttcgcca tttattatca
     2401 tacaaaatta tacaaatgga tataatatta tttttgccac ttcaaccaat tatttggtaa
     2461 taaattttat tttacaaatg tgaactttat tttaagaaat ttaaatttta ctaaaggctc
     2521 tgtaacgcca tttatatttt aattcatgca aaaagaaatt tcattaaaaa ttacaccatc
     2581 ggaagctgct agtaatacaa ctattacaga aattattgct aaaactgttg gagtgagtcc
     2641 caatgaagta tttggttttt ataaattaaa acaaagtata gatgcaagaa gtagaaaaca
     2701 aatttgggta aatattacag taaatgcatt cataaacgaa aaatttattg aaagaaatat
     2761 tgaaaaaatt aatttccaag atgttagcaa cagtaataaa aaagtaatta tagttggtgc
     2821 tggacctgca ggaatttttg cagcattaaa gcttattgaa ttaggtataa aaccaattgt
     2881 tgtagaaaga ggcaaagatg taagaagtag aagaagagat ttagccatat taaataaaga
     2941 aggcaaaata aataccgaga gtaattattg ttttggagaa ggaggtgctg gaacctatag
     3001 tgatggtaaa ctttacacaa gaagcaataa aagaggaagt atagacagaa tactaaattt
     3061 atttgtacag tttggtgcag aagaaaaaat tttataccaa gcccacccac acataggtac
     3121 aaacaaatta ccaagtatta taactgcaat gagagagcaa attataaatt gcggtggcga
     3181 agtgc
//
LOCUS       DAOQHP010000264         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19930_length_3180_cov_3.040640, whole genome shotgun sequence.
ACCESSION   DAOQHP010000264 DAOQHP010000000
VERSION     DAOQHP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19930_length_3180_cov_3.040640"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLY81_08330"
     CDS             complement(<1..209)
                     /locus_tag="PLY81_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30269.1"
                     /translation="MKAKIFYSILLVSILTSCSSAYKTSQTPDDVYYSPAEGNYYVEK
                     EVKTEDEDYLRMKVRNNRFRTIDDY"
     gene            446..1924
                     /gene="proS"
                     /locus_tag="PLY81_08335"
     CDS             446..1924
                     /gene="proS"
                     /locus_tag="PLY81_08335"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNF30270.1"
                     /translation="MSKEITSRQQDYSQWYNDLILKGGLADYSAVRGCMVIKPYGFAL
                     WENMRDVLDKMFKDTGHVNAYFPLFVPKSLFEAEEKNAEGFAKECAIVTHYRLKADPN
                     NKGKLMVDPEAKLEEELVVRPTSEAIIWNTYKGWIQSYRDLPILVNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHSTKEEAIIETEKMLDVYADFVENWMALPVIKGIKTANERF
                     AGAEETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFSDKNNKLEYVWATSWGVST
                     RLIGGLVMAHSDDDGLILPPKIAPIQVVIVPIYKTENEKALIDEKVNTLVNQLKVNNI
                     RVKYDDNDNARPGWKFAEYEMKGVPIRLAIGARDLANNVVEIARRDTKEKQSVSMDEL
                     NIYIQNLLATIQENMFNKAKNFRDEHITNVNTWDEFITTLDNTTGFVNAHWDGTPETE
                     EKIKELSKATIRCIPLNNKEEAGNCILTGKPSKQRVLFARAY"
     gene            complement(1980..2435)
                     /locus_tag="PLY81_08340"
     CDS             complement(1980..2435)
                     /locus_tag="PLY81_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNF30271.1"
                     /translation="MKSYQGKIICNTVLMNDENFIDTTIFITEHNQNGAIGFIINKPF
                     YKSLNQLEEFKNCLAVPLYFGGPVDNEHLFFIHQRNDLFKGGTLITNNFYFGGDFKQA
                     ITLLNQQLITTSDIKIFIGYCGWNMYELEEEINEGCWTVDTYNINLVFQ"
     gene            2667..>3180
                     /locus_tag="PLY81_08345"
     CDS             2667..>3180
                     /locus_tag="PLY81_08345"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNF30272.1"
                     /translation="MDRKVTNIGVLTSGGDSPGMNAAIRAVTRTGLYHGLGVYGIMRG
                     YAGMIEGDIKKLDGISVANIIQRGGTFLKTARSKEFHTYEGRKKAYNNLKQLNIDGLV
                     IIGGDGSFNGAQIFSNEFDIPCIGLPGTIDKDIAGSDTTIGFDTAVNTAVQAIDKIRD
                     TMDAHDRLFIV"
BASE COUNT         1157 a          401 c          576 g         1046 t
ORIGIN      
        1 ctataatcat caatagttct aaacctattg tttcgcactt tcattcttaa ataatcctca
       61 tcttctgttt taacttcttt ttctacataa tagttcccct ctgctggtga ataataaaca
      121 tcatcgggtg tttggcttgt tttataggca gatgaacagg aagttaaaat acttaccaaa
      181 agtatagaat agaaaatttt agctttcata ataaatagtt ttaaatttag tataatgcga
      241 agttgcatac atttgccacc gtttagtatt tatttacttt tatatttaat acaaatgtgg
      301 tgccgtaaaa tttactaaca caagttagta tgttaaaatt tttaaaatga ggataaaagt
      361 tataaaccct gaagggtgcg acgccaagat agttaaataa ataactaaaa gctgactaac
      421 aaaaaaaata cattattata attttatgag taaagaaatt acaagtaggc aacaagatta
      481 tagccaatgg tataacgacc ttattttaaa aggaggtctg gcagattata gtgctgtaag
      541 gggttgtatg gttattaaac cttatgggtt tgcactttgg gaaaatatga gagatgtgtt
      601 ggataaaatg tttaaagata cagggcatgt aaatgcttat tttcctttgt ttgtacccaa
      661 aagtttattt gaggctgaag aaaaaaatgc ggaaggtttt gctaaagaat gtgctatagt
      721 tacccattac agattgaaag cagaccctaa taataaaggg aaattaatgg tagatcctga
      781 agccaaatta gaggaagaat tagtcgttcg ccctaccagt gaagctatta tttggaatac
      841 ttacaaagga tggatacaat cttacagaga tttaccaatt ttagtaaatc aatgggcaaa
      901 tgtggttcgt tgggaaatgc gtacaagatt atttttacgc actgctgagt ttctttggca
      961 agaaggacat acagcacatt ctactaaaga agaggccatt attgaaactg aaaaaatgtt
     1021 agatgtatat gccgattttg tagaaaactg gatggcttta cctgtaatta aaggaattaa
     1081 aacggcgaat gaaagatttg ccggagcaga agaaacctat tgtattgagg ctttgatgca
     1141 agatggaaaa gcattacaag ctggaacatc tcatttctta ggtcaaaatt ttgctaaagc
     1201 atttgatgtt aaatttagcg ataagaacaa taagcttgaa tatgtttggg ctacaagttg
     1261 gggtgttagt accagattaa ttggtggttt agtaatggca cacagcgatg atgatggttt
     1321 gattttacct cctaaaattg ctcctataca agttgtaatt gtacctattt ataaaacgga
     1381 aaatgaaaaa gcattgattg atgaaaaagt aaacacttta gtaaatcaat taaaagttaa
     1441 taatattaga gttaaatatg atgataacga taatgcaaga ccaggctgga aatttgcaga
     1501 gtacgaaatg aagggtgtgc ctattcgttt ggctattggt gcaagagatt tagctaataa
     1561 tgttgttgaa attgccagaa gagatacgaa agaaaagcaa agcgtaagta tggatgaatt
     1621 gaatatttat attcaaaact tacttgcaac aattcaggaa aatatgttta ataaggctaa
     1681 aaattttaga gatgaacata ttaccaacgt aaatacttgg gatgaattta ttacaacatt
     1741 agacaataca acaggctttg taaatgcaca ttgggatggc acacctgaaa cagaagaaaa
     1801 aattaaagaa cttagcaaag ctactattcg ttgtattcct ttaaacaata aggaagaagc
     1861 aggaaattgt attttaacag gaaaaccaag caaacaaagg gttttatttg ctagggctta
     1921 ttaatttata attttatttt atagtaaaaa gctacaataa gtttattgta gcttttgttt
     1981 tattggaata ccagatttat attgtaagta tctactgtcc aacaaccttc atttatttcc
     2041 tcttctaatt catacatatt ccaaccgcag tatccaataa aaatttttat atcgctggtt
     2101 gtaataagtt gttggtttaa taaggtaata gcttgtttaa agtctccacc aaaataaaag
     2161 ttgtttgtaa ttaaagttcc tcctttaaac aagtcatttc tttgatgtat aaaaaataaa
     2221 tgttcgttat ctacaggtcc tccaaaatat aaaggtacag caaggcaatt tttaaattct
     2281 tctaattggt tcagtgattt ataaaatggt ttattaatga taaaacctat agcaccattt
     2341 tggttatgtt ctgtaatgaa aatggttgta tctataaaat tttcatcatt cattaaaact
     2401 gtattacaaa taatttttcc ttggtaagat ttcattcttg taaagttatt gcaaagttta
     2461 taaaattttg ttgattttga agtacaaact aatagtccaa taaaatttta aaatattaaa
     2521 aaaatagctt ttaaccaact tacttgaaag agttacaaac ttcatttttt tgaattgttt
     2581 atataataga gtaatattgc cggctgctaa caaaagaaca atttgcaata taattacctt
     2641 tactagttac tgtaaataag tttgaaatgg atagaaaagt taccaatata ggagtgctaa
     2701 catctggtgg agatagccca ggaatgaatg ctgccataag agctgttacc agaacaggtt
     2761 tataccatgg tttaggagtt tatggtataa tgagaggata tgcaggtatg atagaaggcg
     2821 atataaaaaa attagatgga attagtgttg ccaacattat tcaaagagga ggaacttttt
     2881 taaaaactgc tcgtagtaaa gagtttcata cctatgaagg cagaaaaaaa gcatacaata
     2941 atttaaagca attaaatatt gatggtttag taattatagg aggagatggt agttttaatg
     3001 gtgctcaaat tttttctaac gagtttgata ttccttgtat tggcttacct ggaaccatag
     3061 ataaagatat agctggtagt gatactacta ttggttttga tacagctgta aatacagcag
     3121 tacaagcaat tgataaaatt agagacacta tggatgccca tgacaggtta tttattgtag
//
LOCUS       DAOQHP010000265         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_19999_length_3174_cov_4.099391, whole genome shotgun sequence.
ACCESSION   DAOQHP010000265 DAOQHP010000000
VERSION     DAOQHP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19999_length_3174_cov_4.099391"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLY81_08350"
     CDS             <1..448
                     /locus_tag="PLY81_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF30273.1"
                     /translation="DKERSEIIITGRTKHFLSLVGEHLSVENMNKAIKLVGEEFNISI
                     PEFTVAGVPYQSFFAHHWYIACNDKIDADTLALKIDEHLKVLNDDYAVERKSALKEIF
                     LTILTEDKFMQFMKLKGKIGGQHKFPRVLKGKMLEDWHLFLKDELK"
     gene            511..1164
                     /locus_tag="PLY81_08355"
     CDS             511..1164
                     /locus_tag="PLY81_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNF30274.1"
                     /translation="MIEAIISGLTLGLLLSISVGPVIFAVIRQSLSNGHIGGYAFIAG
                     VSASDVTLVLVCNVLSTIFNHLLANKMIIGVAGSCFLIAIGIYTFFFKKVVFSNENSG
                     TEKKFKKTELAGIFLSGFFMNLLNPGVFIFWLAATAKIHAQAALQLHSLRYIVTVFFI
                     CLAVVLATDIAKVLLAGKIRSKLTPHNIHIINKISGVIFIVFGMFLIWGVMFHGDKI"
     gene            complement(1175..1552)
                     /locus_tag="PLY81_08360"
     CDS             complement(1175..1552)
                     /locus_tag="PLY81_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HNF30275.1"
                     /translation="MKLFSTLKMSYHIYGLKNCNTTQKAIQWFKQHKLAYKFINNRTE
                     PISKSKLELWCNQVSWEKLLNKKGTAFKLLHPAIQQSATTQEKAIEIMLARPSTIKRP
                     LIEKDNKIITLGFSQVFFEIHYL"
     gene            complement(1560..2291)
                     /locus_tag="PLY81_08365"
     CDS             complement(1560..2291)
                     /locus_tag="PLY81_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584500.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNF30276.1"
                     /translation="MKNYLPHFIIAIAIIIFGFIISNAYIYKFTSTETIVVTGLAEKD
                     FTSDLIVWKGSFTRTGLDLKEVYAQIKQDEASIKNYLKEKGLPDSSVIFSSFDVLKNY
                     QSKYAENGTITGNIFTGYTLTGEVKIESMNLPLVEKISREVTELLQKGIEFNSQKPSF
                     YYTKLHDLKIDLLANASKDAKLRAETIAKNSGTNINSLKRATSGVFQITGKNDNEEYS
                     YGGSFNTTSKFKTASITIRTEYLCK"
     gene            complement(2371..2721)
                     /locus_tag="PLY81_08370"
     CDS             complement(2371..2721)
                     /locus_tag="PLY81_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30277.1"
                     /translation="MKFITALVLTALLCFAIGLFTNLPWYSFVLCSLIVAVAIHQKPF
                     KAFLAGFIAAFLLWFIMAWLKDSANEHLLSKKVAEILPLNGNYHLLIILSGLIGGLLA
                     GMGALTGSFLRKIK"
     gene            complement(2732..>3174)
                     /locus_tag="PLY81_08375"
     CDS             complement(2732..>3174)
                     /locus_tag="PLY81_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF30278.1"
                     /translation="NINTKNKKETLAEIVTQAFKNASKTLDSLEKINKLEWVNYKDTK
                     VQHLLQLPALSKLHLPIGGGVHIINATSTTHGPSWRMIVHLTDKIEAYGVYPGGQNGN
                     PGSKYYSNFVDTWAAGKYYNIIFISKQEARKDTRMKWHTVFVNG"
BASE COUNT         1072 a          440 c          464 g         1198 t
ORIGIN      
        1 agataaagaa agaagtgaaa taataattac cggaagaaca aaacattttt taagcctggt
       61 aggagagcat ttgagtgtag agaatatgaa caaagctatt aaattagttg gagaggagtt
      121 taacattagt attccagaat ttacagttgc tggtgtacct tatcaaagtt tttttgctca
      181 tcattggtat attgcctgca atgataagat tgatgcagat acattggctt taaaaataga
      241 tgaacattta aaagtattaa atgatgatta tgcagtggaa agaaaaagtg cattaaaaga
      301 aattttctta actatactta ctgaagataa gtttatgcag tttatgaaac taaaaggaaa
      361 aataggaggg caacataaat ttccaagagt attaaaagga aaaatgttag aagattggca
      421 tcttttttta aaagatgaat taaaataaaa ttttggcatt aattttttgt aacaataatt
      481 cttataaaac ttatttagga aataaataaa atgatagaag caattataag tggactaaca
      541 ttaggattac tactttccat atcggttggt ccggttattt ttgcagtaat tagacaaagc
      601 ttaagtaatg gccatatagg aggttatgca tttattgctg gagtttctgc cagcgatgtt
      661 acactagttt tagtttgcaa tgtattaagt acaattttta atcatttact agctaataaa
      721 atgattatag gtgtagcagg tagttgtttt ttaattgcta taggtattta tactttcttt
      781 tttaaaaaag ttgttttttc aaatgaaaat tctggtaccg aaaaaaaatt taaaaaaaca
      841 gaattagctg gcattttttt atctggcttt tttatgaatt tacttaatcc aggtgtattt
      901 attttttggt tagcagccac agctaaaata catgcacaag ctgcactaca gctacattct
      961 ttaagataca ttgtaactgt tttttttatt tgtttagctg tagttttagc aacagatatt
     1021 gcaaaagttt tattggcagg aaaaataagg tcaaagctta caccacacaa tattcatatc
     1081 atcaataaaa tttctggtgt aatttttatt gtttttggta tgtttttaat atggggtgtc
     1141 atgtttcatg gagataaaat ttaagacaga tatctcataa ataatgtatc tcaaaaaaaa
     1201 cttggctaaa acctaaagta ataattttat tatccttctc tatcaatggc ctttttatgg
     1261 tacttggcct tgcaagcatt atttcaattg ctttttcttg tgttgtagca gattgttgaa
     1321 tagcaggatg taacaattta aatgcagtac cttttttgtt caataacttc tcccaactta
     1381 cctgattaca ccaaagctct aatttgcttt tggatattgg ctctgttcgg ttattaataa
     1441 atttataagc taatttatgc tgcttaaacc attgtattgc tttttgagtg gtattgcaat
     1501 tttttaaacc ataaatatga tagctcattt ttaaagtaga aaataatttc aataaaaatt
     1561 tatttacaca aatattctgt tctaatagtt atggaggcag ttttaaattt agaagtagta
     1621 ttaaaagagc ctccatagct gtactcttca ttatcatttt ttccggtaat ttgaaaaacg
     1681 ccagaagtag ctctctttaa gctattaata ttagtaccag agtttttggc aattgtttcg
     1741 gctcttaact ttgcatcttt tgacgcatta gccaataaat cgatttttaa atcatgcaat
     1801 tttgtatagt aaaaagatgg cttttgcgag ttaaattcaa ttcccttttg caataattca
     1861 gttacttctc tcgaaatttt ttcaaccaat ggtaaattca ttgattcaat tttaacttcg
     1921 ccagttagtg tatatccagt aaaaatattg cctgtaattg ttccattttc ggcatattta
     1981 gattgatagt ttttcaatac atcaaaagac gaaaaaatta cactactatc tggtagtcct
     2041 ttttctttca aataattttt aatgcttgct tcatcttgtt ttatttgagc atatacttct
     2101 tttaaatcta atccagttct ggtaaagctt cctttccaaa caattaaatc gcttgtaaag
     2161 tctttttctg ctaaacctgt tacaacaatt gtttcggtag aagtaaattt atatatgtaa
     2221 gcattactga taataaaacc aaaaataatt atagctattg caattataaa atgcggtaag
     2281 taattcttca ttataattaa ttttatttaa gatgaagtta tatttaaaaa tagataaatt
     2341 actctaatat acttttactt taacttttaa ttattttatt tttcttaaaa agcttcctgt
     2401 taaagctccc atgccagcta ataaaccacc aatcaatcct gatagtatta ttaataaatg
     2461 ataattgcca ttgaggggta aaatttcagc aaccttttta gaaagtaaat gttcattggc
     2521 agagtctttt aaccaagcca ttataaacca taataaaaat gcagcaataa aacctgctaa
     2581 aaatgcttta aatggttttt gatgaatggc aactgccaca attaaactac ataacacaaa
     2641 actataccag ggtaagtttg taaataagcc aattgcaaaa cataataatg ctgttaaaac
     2701 taatgctgta ataaatttca ttgtttaaat tttatccgtt tacaaaaact gtatgccatt
     2761 tcattcttgt atcttttctt gcttcttgct ttgaaataaa aataatatta tagtatttac
     2821 cggctgccca agtatctaca aaattactgt aatatttact tcctgggttt ccattttgcc
     2881 caccaggata aacgccatag gcttctattt tatctgttaa atgtacaatc attctccaac
     2941 ttgggccatg tgtggtagaa gttgcattga taatatgaac accaccacca atgggcaaat
     3001 gtaatttgct taatgcaggt aattgtaata agtgctgtac tttggtatct ttatagttca
     3061 cccattctaa tttatttatt ttctctaaac tgtctaaagt tttagaagca tttttgaatg
     3121 cttgtgtaac aatttctgct aaggtttcct ttttattttt tgtattaata ttat
//
LOCUS       DAOQHP010000266         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20013_length_3173_cov_4.206863, whole genome shotgun sequence.
ACCESSION   DAOQHP010000266 DAOQHP010000000
VERSION     DAOQHP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20013_length_3173_cov_4.206863"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..796)
                     /locus_tag="PLY81_08380"
     CDS             complement(200..796)
                     /locus_tag="PLY81_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF30279.1"
                     /translation="MIFVFLNSFFEWLDKADKQLFLLVNKEWTNTFLDNNLPWYRDST
                     TWLPLYLFLLLFVIFNYGKKSLPWIIAVAITSTISDQISSNFLKNLIERPRPCNDVFI
                     QHLGRLLLNRCPSSFSFTSSHAVNHFTAAMFIFTTLKPAFGKYATLFPIWAATICYAQ
                     VYVGVHYPLDVFAGAIAGCCIGIFAGKWYNKYYSLQTI"
     gene            865..2874
                     /locus_tag="PLY81_08385"
     CDS             865..2874
                     /locus_tag="PLY81_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF30280.1"
                     /translation="MKKLKIPRYIRWIAQTGIMFLILMSLMRWVLVLTYRTPTEKSVS
                     LIDAYWLGFRFDLRFVSITMLLLYLVGTIPYLHPINKKWGNRVSFIIWSVFIVLTSIF
                     YAADFAHYSYLRQRLNANILSFLHDAKISFGMMWQSYNLGWGILGLIVIVFILLAMVK
                     LSYNHILSKANVSTKKSSKVWGVLFVLLLAFGIVGNVVYKAGQYPLRWSNAYTLGNDY
                     AANISLNPFQSFFSTLKFKDVTFDEEKVKKHYEWMSAYLDVPLNQRDSNKLNFNRIQE
                     LDSSNKMAQHIQNVVLVICESFSAYKSSMYGNPLNTTPFFAEMCKEGVFFNRAFSPAY
                     GTARGVWAIVTGIPDVQFNKTSSRNPATVNQHTIINDFNQHEKFYFLGGSSSWANIRG
                     LLTNNIKDLRLYEQGSYSSKEIDVWGISDKNLFLEANKTLAQQTKPFFAVIQTADNHR
                     PYTIPKEDLKEFKIQKIKNAQLKKYGFESEEEYNAFRYTDFSFQKFIEAAKKEKYFNN
                     TLFVFIGDHGIRGDAGDMLPKAFTTQGLTNMHVPLLFYAPGILPAKEITFPASQLDVL
                     PSIAYLCNIPYTNTTMGRNLFKLKNTNDNYTFIIDEGAKIIGALNDNYFFNYQLKDKK
                     VENFVSLQHNSPVTNDSIKKQLHFITDAFYETARYMLLNNKKQNP"
     gene            2877..>3173
                     /locus_tag="PLY81_08390"
     CDS             2877..>3173
                     /locus_tag="PLY81_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30281.1"
                     /translation="MLIKWKVPKTIQWVIKLFFIFLFIFTLFRISTYIAFKPDDIKLN
                     DVWSCFAMGLRFDIRWISILLIPIVLISLIPNFSPYSSTTTKRFWIVYLAICTFL"
BASE COUNT         1137 a          445 c          511 g         1080 t
ORIGIN      
        1 cattgatttg gcgcaaaacg tacactgaaa acataccatg ttcaaaaatg ttttgaatta
       61 ggaattacaa ccgaacaatt gtacgaaatt tttgctgtag caaatattgt tggaggaaca
      121 attgttatac cacatactag aagagctgct gaatattggg aagaattaat aaaacagtaa
      181 acaatttgta tttgaaaaat tatatagttt gtaaactgta atatttatta taccattttc
      241 ctgcaaaaat accaatacaa caacctgcta ttgcaccagc aaaaacatct aaaggataat
      301 gaaccccaac atacacttgt gcatagcaaa tagttgctgc ccaaattgga aaaagagtag
      361 catattttcc aaatgctggc tttagtgttg taaatataaa catagctgca gtaaaatggt
      421 ttacagcatg agaggatgta aaactgaaac tagatggaca acgatttaat aaaagcctgc
      481 caaggtgttg tataaacaca tcattacaag gccttgggcg ttctattaag ttttttaaaa
      541 aattactact tatttgatca gagatggtgg aagttattgc cacagcaatt atccagggca
      601 acgatttttt tccgtaatta aaaattacaa atagtaataa aaataagtac agcggtagcc
      661 atgttgtaga atctctatac caaggtaaat tattatctaa aaaggtatta gtccattctt
      721 tattaactaa taagaacagt tgcttatctg ccttatcaag ccattcaaaa aaactattta
      781 agaaaacgaa tatcattgct gtaaagataa ttgcttaatg aatggaataa aagcaatgtt
      841 tttattttat acttttgttg ttgaatgaaa aaactaaaaa ttcccagata tataagatgg
      901 atagctcaaa caggcattat gtttttaatt ttaatgagct taatgcgttg ggtacttgta
      961 ctaacttata gaacgccaac agaaaaatct gtaagtttaa tagatgctta ttggcttggg
     1021 tttaggttcg acttaaggtt tgtatccata accatgttat tattgtactt agttggtaca
     1081 ataccttatt tacatcctat aaacaaaaaa tggggcaata gagtttcgtt tattatttgg
     1141 tcggttttta ttgtacttac cagtattttt tatgctgcag attttgcaca ttattcttat
     1201 ttacgccaaa ggcttaatgc aaatatttta agctttctac acgatgccaa aatatctttt
     1261 ggaatgatgt ggcagagcta caatttaggt tggggcattt tagggctaat agttattgta
     1321 tttattttat tagctatggt aaaacttagt tataaccata ttttaagtaa ggctaatgtt
     1381 tcaactaaaa aaagtagtaa agtatggggt gttctttttg tgctgctatt ggcttttggc
     1441 attgttggca atgtagttta caaggcagga caataccctt tacgttggag taatgcttat
     1501 actttgggca acgattatgc tgcaaatatt tctctcaatc cctttcaatc tttttttagt
     1561 accctaaaat ttaaagacgt aacttttgat gaagagaaag taaaaaagca ttatgaatgg
     1621 atgagtgctt atttagatgt tcctttaaat caaagggaca gtaataaatt aaactttaat
     1681 agaattcaag aattggatag cagtaataaa atggcacaac atatacaaaa tgttgtattg
     1741 gttatttgcg aatctttttc tgcttataaa agtagtatgt atggcaaccc tttaaatact
     1801 acgccatttt ttgcagaaat gtgtaaagaa ggtgtttttt ttaacagagc tttttctcct
     1861 gcttatggca ctgctagagg tgtttgggca attgttacag gcataccaga tgtacagttt
     1921 aataaaacat ctagccgtaa ccctgcaact gtaaatcaac acactattat taatgatttt
     1981 aaccaacacg aaaaatttta ttttttagga ggtagtagta gttgggcaaa tattagaggg
     2041 ctattaacaa ataatataaa agacctgagg ttgtatgaac aaggtagtta ctcatcaaaa
     2101 gaaattgatg tttggggcat tagtgataaa aacctttttt tagaagctaa taaaactctt
     2161 gcacaacaaa caaaaccatt ttttgcagtt attcaaactg ccgataatca tagaccatac
     2221 accataccta aagaagattt aaaagaattt aaaattcaaa aaattaaaaa tgcccaacta
     2281 aaaaaatatg gttttgaaag cgaagaggaa tacaatgctt ttagatatac agattttagt
     2341 tttcaaaaat ttatagaggc tgctaaaaaa gaaaaatact ttaataacac tttatttgtt
     2401 tttattggag accatggtat tagaggagat gcaggagata tgttgccaaa agcctttacc
     2461 acacaaggct taaccaatat gcatgtgcct ttgttatttt atgcaccagg cattttacct
     2521 gcaaaagaaa taacttttcc agcatcgcaa ttagatgtgt tgccttctat tgcttattta
     2581 tgtaatatac catatacaaa tactacaatg ggaagaaact tatttaaact aaaaaataca
     2641 aacgataatt atacttttat aattgatgaa ggtgcaaaaa ttattggagc attaaacgat
     2701 aattactttt ttaattatca gctaaaagat aaaaaagtag aaaattttgt tagccttcaa
     2761 cacaattcac cagttacaaa cgatagtatt aaaaagcaat tacattttat tacagatgct
     2821 ttttatgaaa cagcaaggta tatgttatta aataataaaa agcaaaaccc ataatcatgc
     2881 ttattaagtg gaaagttccc aaaacaatac agtgggttat aaaattattt tttatttttt
     2941 tattcatatt tacacttttc agaatttcta catatatagc ttttaaacca gacgatatta
     3001 agctaaatga tgtttggagt tgctttgcaa tgggtttaag atttgatatt cgctggataa
     3061 gcattttatt aattccaatt gtattaatta gtttaatacc taatttttct ccttacagct
     3121 ctactactac caaacggttt tggatagtgt atttggcaat ttgtactttt tta
//
LOCUS       DAOQHP010000267         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20263_length_3149_cov_3.628636, whole genome shotgun sequence.
ACCESSION   DAOQHP010000267 DAOQHP010000000
VERSION     DAOQHP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20263_length_3149_cov_3.628636"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1879
                     /locus_tag="PLY81_08395"
     CDS             56..1879
                     /locus_tag="PLY81_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF30282.1"
                     /translation="MSNAAVLHSNIGTMHDDHSAHHEHHHQETFINKYVFSQDHKMIA
                     KQFLITGMVWALIGGFLSVLFRMQLGFPEESFPWLEDLLGKWWAAGGRITAQGYYALV
                     TTHGTVLVFFVLTAGLSGTFANFLIPLQIGARDMASPFMNMLSYWFFFAAGVIMLASI
                     FVQTGPFSGGWTAYPPLSALGDASPGSKTGMDMWIISMALFVVSSLLGGLNYIATILN
                     MRTKGMSMTRMPLTIWALLFTAILGVLSFPVLLSGFILLIFDRNFGTSFYLSDIFLAS
                     TNTALPNEGGSAILFQHLFWFLGHPEVYIIMLPAMGMVSEIISINSRKPIFGYLAMVG
                     SLFAIATLAFLVWAHHMFVTGLNPFLGSIFALLTLLIALPSSIKVFNWLTTLWRGNIR
                     LTPGMLFAIGFVSMFISGGLTGIWVGNATLDIHLHDTYFVIAHFHLVMGVASMFGMFA
                     GVYHWFPKMYGRHMNNTLAYIHFWITLIGAYLIFWPMHYQGLAGMPRRYYDYSIWESF
                     KQFSELNRFISIVVFITFIAQLLFLINFFYSIWKGRKATNLNPYGANTLEWTTPINPG
                     HGNWPGEIPEVHRWAYDYGKDGKDFITQTTPVGEEESTEHH"
     gene            1984..2886
                     /gene="cyoE"
                     /locus_tag="PLY81_08400"
     CDS             1984..2886
                     /gene="cyoE"
                     /locus_tag="PLY81_08400"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNF30283.1"
                     /translation="MTDKSLTYSTSFLSKLHDYKELTKFTLTFTVVFTCVISYLLAPS
                     IISYNLKMILLLFAAGLLITGSANAINQAVEKNSDALMKRTANRPVAAGRMSQKEAYT
                     FAFLTGAIGVFIMWYFFNTSSALLSAFSLFLYSFIYTPLKTKNSIAVLIGGLPGALPC
                     LIGWVAAEDSFSIGGWILFAIQFIWQFPHFWAIAWVAHKDYTAAGFKLLPSNEGPTKY
                     TAIQTIMYSVLMIPIGLLPYYFNITGSVSMYIILACNIAMVIQSIRLYNQMNVKAAKR
                     VMFSSYIYLPIVYLAMLADKIAIL"
     gene            2901..>3149
                     /locus_tag="PLY81_08405"
     CDS             2901..>3149
                     /locus_tag="PLY81_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNF30284.1"
                     /translation="MQIAMNNQPTTHRIHPHKFTLWMAIGSICMMFAGLTSAYIVKRN
                     QDNFLEFDLPIIFWLSTIVIILSSVTMHLALKAYKARKT"
BASE COUNT          914 a          495 c          559 g         1181 t
ORIGIN      
        1 gcaaaagttt ttttaataat tttttagaac actatacaag aaataagtat ttattatgag
       61 taacgcagca gttctacatt caaatattgg taccatgcat gacgatcatt ctgctcatca
      121 tgaacaccac caccaagaaa cttttataaa caaatatgta tttagccaag accataaaat
      181 gattgctaaa cagtttttaa taactggtat ggtatgggct ttaattggtg gctttttaag
      241 tgttttattt agaatgcaat taggttttcc agaagagtct tttccttggt tagaagattt
      301 attaggcaaa tggtgggctg caggtggtag aattactgct caagggtatt atgccttagt
      361 tactacacac ggtactgtac tagttttctt tgtattaact gctggcttaa gtggtacttt
      421 tgcaaacttc ttaattcctc tgcaaattgg tgcaagagat atggcttcgc cttttatgaa
      481 tatgcttagc tattggtttt tctttgcagc tggggttatt atgttagctt ctatttttgt
      541 tcaaacaggt ccttttagtg gtggttggac agcctatcct cctcttagtg ctttaggaga
      601 tgcttctcca ggttctaaaa ctggtatgga tatgtggatt atttctatgg ctttgtttgt
      661 tgtatcctct ttattagggg gcttaaatta tattgctacc attttaaaca tgcgtaccaa
      721 aggtatgagc atgactagaa tgccattaac aatttgggct ttattattta cagctattct
      781 aggcgtttta tctttccctg tgttattatc tggttttatt cttttaattt ttgatagaaa
      841 ttttggaaca agtttttatt tatctgatat cttcttagca tctaccaata cagccttacc
      901 aaacgaaggt ggtagtgcca ttttattcca acatttattc tggttcttag gccacccaga
      961 ggtttatatt attatgcttc ctgcaatggg tatggttagt gaaattatca gtataaattc
     1021 tcgtaaacct atttttggtt atttagcaat ggtgggtagc ttatttgcaa ttgctacact
     1081 agcattttta gtttgggcac accacatgtt tgttacaggt ttaaatcctt tcttaggttc
     1141 aatttttgct ttactaactc tattaattgc actacctagt tctattaaag tatttaactg
     1201 gctaactaca ttatggcgtg gtaatattag gttaacgcct ggtatgctgt ttgccattgg
     1261 ctttgttagt atgtttatat ctggtgggtt aactggtatt tgggtaggta atgcaacttt
     1321 agatattcat ttacacgata cttactttgt tattgctcac ttccacttag taatgggcgt
     1381 agctagtatg tttggaatgt ttgcaggtgt atatcattgg tttcctaaaa tgtatggaag
     1441 acacatgaac aatactttag cgtatataca cttttggata acattaattg gtgcttacct
     1501 aattttctgg ccaatgcact accaaggttt agctggtatg cctcgtagat attatgatta
     1561 cagcatttgg gaaagcttta aacaattcag cgagttaaac aggtttatta gtattgtggt
     1621 atttatcact tttatagctc aattattatt tttaattaac tttttctact ccatttggaa
     1681 aggaagaaaa gctactaact taaaccctta tggtgccaat actttagagt ggacaacacc
     1741 aattaaccct ggtcatggta actggcctgg cgaaatacca gaagtacacc gttgggctta
     1801 tgattatggt aaagatggta aagactttat tacccaaacc actcctgttg gagaggaaga
     1861 aagtacagag caccactaaa atttatggta tagtaacatc taaattataa atgatttatg
     1921 tagaaaatgc cctgaacttt tcggggtatt tttttgaata attattttat ttcttaattc
     1981 ttaatgacag ataaaagcct aacatattct acttcttttt tatctaagct acacgattat
     2041 aaagagctca ctaaatttac gctaactttt accgtagttt ttacctgtgt tatcagttat
     2101 ttattagccc caagcattat tagttacaac ctaaaaatga ttttgttact ttttgctgct
     2161 gggttattaa taacaggtag tgcaaatgcc atcaatcagg ctgtagaaaa aaactctgat
     2221 gctttgatga aaagaactgc aaataggcct gttgctgctg gcagaatgag ccaaaaagaa
     2281 gcctatactt ttgctttttt aacaggtgca ataggtgtgt ttattatgtg gtattttttc
     2341 aatacctctt ctgccttact ttctgccttt agtttatttc tttattcatt tatttatacg
     2401 ccattaaaaa ctaaaaattc tattgcagtt ttaattggtg gtttaccagg tgccttgcct
     2461 tgtttaattg gttgggttgc tgccgaagat agttttagca ttggcggatg gatactgttt
     2521 gccatacaat ttatttggca atttccacat ttttgggcta ttgcatgggt ggctcataaa
     2581 gattatacgg ctgctggttt taaactttta ccaagcaacg aaggaccaac taaatatact
     2641 gccatacaaa caattatgta tagcgtttta atgattccta tagggctttt accatattac
     2701 tttaatataa caggttccgt gagtatgtat attattttag cttgtaatat tgcaatggtt
     2761 atacaaagta taagattgta taaccaaatg aatgtaaaag cagcaaaaag agtaatgttt
     2821 agtagttata tttatttacc aattgtatat ttagcaatgc tagccgataa aatagctata
     2881 ctttaaattt taattgtatt atgcaaatag ccatgaacaa tcaaccaact acacatagaa
     2941 ttcatcccca caaatttacc ttatggatgg ccattggtag catttgtatg atgtttgcag
     3001 gcttaacgag tgcctatatt gttaaacgca atcaagataa ttttttagaa tttgatttac
     3061 ccattatttt ttggttaagt actattgtaa ttattttaag cagtgtaact atgcaccttg
     3121 ctttaaaagc ctataaagca agaaaaaca
//
LOCUS       DAOQHP010000268         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20265_length_3149_cov_3.333549, whole genome shotgun sequence.
ACCESSION   DAOQHP010000268 DAOQHP010000000
VERSION     DAOQHP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20265_length_3149_cov_3.333549"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLY81_08410"
     CDS             complement(<1..686)
                     /locus_tag="PLY81_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF30285.1"
                     /translation="MKRIIISGYAHSVLQEMLSNAGFEVIYNPTISYQELLNEIEPAV
                     GLITTTRIKADEMLLRKATNLKFIGRLGSGMDLIDVEFAKQKNIKCLNSPEGNRNAVA
                     EHTLALLLNLMNKINISYDALKQNIWAREENRGDELEGKIVGIIGFGNTGKRFAELLQ
                     PFNVTVLAYDKYKFGFASNYIKQASFEQICKQANVISFHVPLTAETHYYANDSFFNSL
                     QQQPYFLNTC"
     gene            complement(691..1458)
                     /gene="rsmA"
                     /locus_tag="PLY81_08415"
     CDS             complement(691..1458)
                     /gene="rsmA"
                     /locus_tag="PLY81_08415"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNF30286.1"
                     /translation="MQYTLKKALGQHFLKDENICKNITDVINQSLNETTISNLLEVGP
                     GAGAITKYLLQIPGINFKVVELDAEKVAYLNATYTAIQSKIIHQNFLEMHAPFDEKFM
                     VVGNFPYNISSQILFKILEWKQNVPVVVGMFQKEVAQRVAAQPGSKTYGIISILIQAY
                     YDVEYLFDVSANCFNPPPKVMSGVIQLKLKDKTFSKRSDKDFINLVKTAFNQRRKMLR
                     NAVKNLFSEDELKNEIFNKRAEQLSIEDFAALTFKMK"
     gene            complement(1506..2108)
                     /locus_tag="PLY81_08420"
     CDS             complement(1506..2108)
                     /locus_tag="PLY81_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNF30287.1"
                     /translation="MVKNIFIIALMGMVNIVIAQQSSQSNKTISVCLSNSEMKLYNLV
                     MEYRLQNGLPSIPLSASLCFVAQQHCKDLINNKPKTSVCNMHSWSSNGSWSSCCYTPD
                     HKNAACMWNKPRELTNYKGNGYEIAFYVWSSASATYTAEPEEALKGWKGSPGHNDVII
                     NKNIWKSANWNSIGIGIYYGYATIWFGEEKDTETIPQVCN"
     gene            2264..>3149
                     /locus_tag="PLY81_08425"
     CDS             2264..>3149
                     /locus_tag="PLY81_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF30288.1"
                     /translation="MNEIIKVTNLTKQFKQITAVNNLSFNVNSGDVYGFLGQNGAGKS
                     TTIRMLLTLIQPTNGSIEIFGLPLQNNRNEILKNIGAVIEKPDVYKYLSAYDNLKLFA
                     RLNGTRPTHHQLMQQLEIVGLANRAKDKVATFSQGMKQRVGIAIALIHQPQLIILDEP
                     TNGLDPQGIADIRNLIIKLSKEHGKTILISSHLLSEIEQIATRILIIDKGKKIIEDTA
                     TALFNPSQTIVEIETTNNEFALQQLQKSKWLTSIQQHTTNTITFKLDKSQIPTLNSNL
                     VEMQIQLISIKPRHSLEDY"
BASE COUNT         1118 a          527 c          413 g         1091 t
ORIGIN      
        1 ctgcaagtat ttaaaaaata aggctgttgt tgtaaagagt taaaaaaaga atcatttgca
       61 taataatgcg tttctgctgt aagtggcaca tgaaaactaa ttacattagc ttgtttgcaa
      121 atttgctcaa agctggcttg cttgatatag ttagatgcaa acccaaactt atatttatca
      181 taagctaata cagtaacatt aaaaggttgt agaagttcag caaatctttt acctgtatta
      241 ccaaagccaa taataccaac tattttaccc tctaactcat ctcccctatt ttcttctctt
      301 gcccaaatat tttgctttaa tgcatcgtaa cttatgttta ttttattcat aaggtttaac
      361 aacaatgcca atgtatgttc agctactgca tttctatttc cctctggact atttaaacat
      421 tttatatttt tttgttttgc aaactccaca tcaattaaat ccattccgct acctaaccta
      481 cctataaatt ttaaattagt tgcttttctc aaaagcattt catctgcctt aattctagtt
      541 gttgtaatta acccaactgc aggttcaatt tcattcaaca attcttggta gctaatagtt
      601 gggttatata ttacctcaaa gcctgcattg cttaacattt cttgcaatac agaatgagca
      661 taaccagaaa taattattct tttcaatttt ttatttcatt ttaaaagtta gggcagcaaa
      721 gtcttcaata cttaactgct ctgctcgttt attaaaaatt tcattcttta actcatcttc
      781 tgaaaataaa tttttaactg catttctaag catttttctt cgttgattaa aagcggtttt
      841 tactaaatta ataaaatctt tatcgcttct tttgctgaag gttttatctt ttaatttaag
      901 ttgaattaca ccactcataa cctttggtgg aggattgaaa caatttgcag aaacatcaaa
      961 taaatattct acatcataat aagcctgaat taatatacta atgataccgt atgttttgct
     1021 gccaggttgt gctgcaacac gttgggctac ttctttttga aacatgccaa caacaacagg
     1081 tacattctgt ttccattcta atattttaaa taatatttga gaagaaatat tataaggaaa
     1141 attacctacc accataaatt tttcatcaaa tggtgcatgc atttctaaaa aattttgatg
     1201 aattatttta gattgtatag cagtataagt agcatttaag taagctactt tttctgcatc
     1261 taattcaaca actttaaaat ttataccagg tatttgaagc aagtattttg taatagcacc
     1321 agcaccaggt ccaacttcta ataaattact aatagtagtt tcattcaaag actgattaat
     1381 tacatctgta atatttttac atatgttttc atcttttaaa aaatgctggc ctaatgcttt
     1441 ttttagtgtg tattgcattg ctgcaaatgt aacatcaaat aatattgcaa ttaaaatgga
     1501 aaaaattaat tacaaacctg tggtattgtt tctgtatcct tttcttctcc aaaccaaatg
     1561 gtagcataac cataatagat accaatacca attgaattcc aattagccga tttccaaata
     1621 tttttattaa ttataacatc gttatgtcca gggcttccct tccatccttt taaagcttct
     1681 tcaggttctg cagtatatgt ggctgaagcc gaactccaaa cataaaaagc aatctcgtat
     1741 ccattacctt tataattggt tagctctctt ggcttattcc acatgcaagc agcattttta
     1801 tgatctggcg tataacaaca agaactccaa cttccattac tactccaact atgcatattg
     1861 cacacagaag ttttaggttt gttattaatc aaatctttac aatgctgttg tgctacaaaa
     1921 cataaagatg cagaaagagg tatagagggt aatccatttt gcagcctgta ttccatcact
     1981 aaattataca atttcatttc actattactt aaacaaacac ttatggtttt attgctttgt
     2041 gatgattgtt gtgcgataac aatatttacc atacccatta aagcaataat gaaaatattt
     2101 tttaccatta taagttgaat aaaaatttga tacttaaaaa taaggtttct ttttacttaa
     2161 aaaaagcaaa tagtattttt cacatcaatt tatcaaattt attatacaaa acctactatg
     2221 caatatatgt agctactacc tttgccccaa ttggctatat tacatgaatg aaataattaa
     2281 ggttacaaat ttaactaagc agtttaagca aattacagca gtaaataatt tatcttttaa
     2341 tgttaatagt ggtgatgtgt atggcttttt agggcaaaat ggagccggaa aaagtacaac
     2401 cattagaatg ttacttacat taatacaacc cactaatggt agtatagaaa tttttggctt
     2461 accactacaa aataatagaa atgaaatatt aaaaaatatt ggtgcagtaa tagaaaaacc
     2521 agatgtatat aaatacttaa gtgcttacga taatttaaaa ctttttgcca ggctaaatgg
     2581 taccaggcca acacatcatc aactaatgca acaattagaa attgttgggc tagcaaatag
     2641 agcaaaagat aaagttgcaa ccttctctca aggcatgaaa caaagagtag gaattgctat
     2701 tgcattaata caccagccac agttaattat tttagatgaa ccaactaatg gtttagatcc
     2761 tcaaggcata gcagatataa gaaatttaat aataaaatta agtaaagagc atggtaaaac
     2821 tattttaatc tcatcgcacc ttttaagtga aatagaacaa attgcaacaa gaattttgat
     2881 aattgataaa ggtaaaaaaa ttattgaaga tactgctacc gctttgttta acccatcgca
     2941 aacaattgtt gaaatagaaa caaccaataa tgaatttgct ttgcagcaac tacaaaaaag
     3001 taaatggcta acaagcattc agcaacatac tactaatacc attactttta aattagataa
     3061 aagccaaata ccaaccttga atagcaattt ggttgaaatg caaattcagc ttattagcat
     3121 taaaccaaga catagtttag aagattatt
//
LOCUS       DAOQHP010000269         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20361_length_3139_cov_4.029183, whole genome shotgun sequence.
ACCESSION   DAOQHP010000269 DAOQHP010000000
VERSION     DAOQHP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20361_length_3139_cov_4.029183"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="hisS"
                     /locus_tag="PLY81_08430"
     CDS             <1..1174
                     /gene="hisS"
                     /locus_tag="PLY81_08430"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNF30289.1"
                     /translation="DKLIFKILNNGLDRIEKREKSAVEFQKVLEGKNNAAITERALRY
                     DLTIPFARFVAMNHGSLTMPFKRYQIQPVWRADKPQRGRYREFYQCDCDVVGSYNLLN
                     EVELANIYSTVFEKLSIDVEIKINSRKILAALAELCGGADKLIDITIAIDKLDKIGLD
                     NVKEELLQRGLNKLQITTIEKYLNITGNNKEKIIALKQLIGNNETGNKGIDELEYILS
                     FQPKVEIDFTLARGLNYYTGIIFEVKATKVQMGSIGGGGRYDDLTGDFGVPNLPGVGI
                     SFGVDRIYDVMEELKVFPDDLKTSTEVLFFNLGMNESKTSFNLMQQLRDKGVSCELYH
                     ETAKFDKQFKYADKKNIHYAVIIGSKEIEEKTCTLKNLKSGEQHSLQQNELLSFSF"
     gene            complement(1250..>3139)
                     /locus_tag="PLY81_08435"
     CDS             complement(1250..>3139)
                     /locus_tag="PLY81_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30290.1"
                     /translation="SRYMSSTIISNQINLGTKELTKAACCNLSESFETSPTVDVSYAD
                     AVTGIKQIQLLGLAGSYTQTITENTPEYRGVAGNMGFNFIPGPWIENIQVTKGIGSVV
                     NGYESIAGQINVELKKPNQKQPFFINGYVNTMGRMEGNFVTSKQLNDKWSTATLVHAN
                     GVIAKNDMNKDGYLDIPKGHQINIANRWDYSNKNGLGIHWILKGVSDRRIGGNKNFTT
                     ADKLTTNNYGVMLNNDALNATAKIGYIFPKYKHRSIGLILSGSYSNINNVYGLTNYKA
                     KQTGFYSNLIYQSIINNTNHKFKTGLSIVADDVKEHVFNKHYNRKELVPGAFFEYTYT
                     VTPNLSLIAGIREDYHNNFGWITTPRLHVKYDATNNTIFRFAVGTGFRLANIFTENSN
                     AFISSRQFFIDNPTNNYEYGLEPEKAVNFGLSFMQKFRTNNKESFFTLDLYRTSFSQQ
                     TVVDYDKNPQEIHFYNLKGKSFSNTVMAELNFYILQNLATRIAYRFLDVQTDYTTGRL
                     QKPLQSQHRAFINLEYELSKGWLVDFTTQWMSEKRIPSTTANPINKRFAAYSPNYFQM
                     AAQITKKFKSGFELYVGGENLTNYKQHHLFIDGKNPFGNYFDGSLVWGPAIGAMIYTG
                     FRYSF"
BASE COUNT         1070 a          454 c          492 g         1123 t
ORIGIN      
        1 agataaactt atttttaaaa ttttgaataa tggtttggac cgaatagaga aaagagaaaa
       61 atcggctgta gaatttcaaa aagttttaga aggaaaaaat aatgctgcca ttaccgaaag
      121 agctttacga tatgatttaa cgataccttt tgcaagattt gttgcaatga atcatggaag
      181 tttaaccatg ccttttaaac gctatcaaat tcaacctgtt tggagggctg ataagccaca
      241 acgtggcaga tatagagaat tttaccagtg tgattgtgat gtggtaggaa gttataattt
      301 attgaacgaa gtagagcttg ccaacattta ttctactgtt tttgagaaac tatcaataga
      361 tgttgaaata aaaattaaca gcagaaaaat attggctgct ttagcagaat tgtgcggagg
      421 tgcagataaa ctaatagata taactatagc tattgacaag ttagataaaa taggattaga
      481 taatgtaaaa gaagagttat tgcaacgagg attaaataaa cttcaaatta ctactattga
      541 aaaatattta aacataaccg gtaacaataa agaaaaaata atagcattaa agcaattaat
      601 tggtaataat gaaaccggta ataaaggaat tgatgaactt gaatatattt taagttttca
      661 accaaaagtt gaaatagatt ttactttagc cagaggctta aactattata caggtataat
      721 ttttgaagtt aaagctacta aagtacaaat gggtagtatt ggaggtggtg gaagatatga
      781 cgatttaact ggagactttg gggttcctaa tttacctggt gttggtatta gttttggagt
      841 agatagaatt tatgatgtaa tggaagaatt aaaagttttt cctgatgact taaaaacaag
      901 cacagaggtt ttatttttta atttaggcat gaatgaaagt aaaacttctt ttaacctaat
      961 gcaacaatta agagataaag gtgttagctg tgagttgtat cacgaaactg ctaaatttga
     1021 taagcaattt aaatacgccg ataaaaaaaa tattcattat gctgtaatca ttggttcaaa
     1081 agagatagaa gaaaaaactt gtacattaaa aaatttaaaa tcaggtgagc aacacagctt
     1141 gcaacaaaac gaactccttt ctttttcttt ttaatcttgt ttaaactttt caaaaaaata
     1201 cctaccaaat tttactttat ggtaggtatt ttttaagaga atttgtttct taaaaagaat
     1261 atctaaatcc tgtataaatc atagcaccaa ttgctggccc ccatacaagg cttccatcaa
     1321 aataatttcc aaaaggattt ttgccatcaa taaataaatg atgttgttta tagttagtta
     1381 gattttcgcc accaacatat aactcaaaac cgcttttgaa ttttttggta atttgtgctg
     1441 ccatctgaaa atagtttgga gaataggctg caaaccgttt gttaattggg ttagctgtgg
     1501 ttgatggaat tcttttttca ctcatccatt gtgtagtaaa atcaaccaac cagcctttgc
     1561 ttaattcgta ttctaaatta ataaatgctc tgtgctgaga ttgtaatggt ttttgtaatc
     1621 ttcctgttgt ataatctgtt tgtacatcta aaaatctata tgcaattctt gtagctaggt
     1681 tttgcaaaat ataaaaattt agttcggcca ttacagtatt tgaaaatgat tttcctttta
     1741 aattatagaa atgaatttct tgtggatttt tatcataatc tacaactgtt tgctggctaa
     1801 agcttgttct gtataaatct aaagtaaaaa agctttcttt attatttgtt ctaaattttt
     1861 gcataaagct taagccaaag tttactgctt tttcaggctc taaaccatat tcataattat
     1921 tggtagggtt atcaataaaa aattgtcttg agctaataaa tgcattagag ttttcggtaa
     1981 aaatattggc caatctaaat ccagtaccaa ctgcaaatct aaaaatggta ttatttgtgg
     2041 catcatattt tacatgtaac cttggtgtag taatccatcc aaaattatta tgataatctt
     2101 ctcttatacc agctattaaa gataagttag gtgtaacagt ataagtatat tcaaaaaatg
     2161 ctcctggtac cagttctttt ctattataat gtttattaaa aacatgctct ttaacatcat
     2221 cggctactat gcttaagcca gttttaaatt tatggttggt attattaatt atagattgat
     2281 aaattaagtt actataaaaa ccagtttgct ttgctttata attagttaaa ccatacacat
     2341 tatttatatt gctataacta ccagataata ttaaaccaat acttctatgt ttatatttag
     2401 gaaaaatata cccaattttt gcagtagcat ttaaagcatc gttatttagc attactccat
     2461 aattgtttgt agttaatttg tcggcagtag taaaattttt gttaccacca attcttctgt
     2521 cagaaactcc ttttaaaatc caatgaattc ctagcccatt tttattgcta taatcccatc
     2581 tgttagcaat atttatttga tgtcctttgg gtatatctaa ataaccatcc ttgttcatgt
     2641 catttttggc tataacacca ttggcatgca ctaaagttgc agtgctccat ttatcattca
     2701 attgcttgga agtaacaaaa tttccttcca ttctgcccat tgtgtttaca taaccattga
     2761 taaaaaaagg ttgcttttgg ttaggctttt ttaattctac attaatttgt ccagctatac
     2821 tctcgtagcc atttacaaca gagccaatac ctttggttac ttgaatattt tctatccaag
     2881 ggcctggaat aaaattaaaa cccatattgc cagcaactcc tctatactct ggtgtattct
     2941 ctgtaatggt ttgcgtataa ctacctgcta aacctaatag ctgaatttgt tttattcctg
     3001 taactgcatc tgcataactt acatcaactg ttgggcttgt ttcaaaactt tcagataggt
     3061 tacaacaagc agctttggtt aattcttttg tacctaagtt tatttgattg ctgataattg
     3121 tagaagacat atatctgct
//
LOCUS       DAOQHP010000270         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20628_length_3117_cov_3.161659, whole genome shotgun sequence.
ACCESSION   DAOQHP010000270 DAOQHP010000000
VERSION     DAOQHP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20628_length_3117_cov_3.161659"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLY81_08440"
     CDS             complement(<1..783)
                     /locus_tag="PLY81_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNF30291.1"
                     /translation="MFKKILIANRGEIALRVIRTCREMGIKTVAVYSTADKESLHVRF
                     ADEAVCIGKPAGADSYLNIQHIMAAAEITNADAIHPGYGFLAENAKFSQICADHSIKF
                     IGPTPEMINKMGDKITAKETMIAAGVPVVPGSGGLLQSVEQAKDLAKNKVGYPIILKA
                     TAGGGGKGMRVVWKEEELEKAYDTAKAEAAASFKNDGIYMEKFVEEPRHIEIQVAGDQ
                     YGNFCHMSERDCSIQRRHQKLVEESPSPFMTPELRKAMGEAAI"
     gene            complement(809..1291)
                     /gene="accB"
                     /locus_tag="PLY81_08445"
     CDS             complement(809..1291)
                     /gene="accB"
                     /locus_tag="PLY81_08445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNF30292.1"
                     /translation="MDLKQIHELIKIINKSNIGEISIEDKDGKVTIKQKEEQIVTVAA
                     PTPQVYNVAAPAAVAPAQATTTAPTAPAPAAVAPKADNLITIKSPMIGTFYRKSSPDK
                     PPFVEVGTDVAPGSVVCIIEAMKLFNEIESEVKGTIVKVLVDDASPVEYDQPLFLVEP
                     "
     gene            complement(1466..1831)
                     /locus_tag="PLY81_08450"
     CDS             complement(1466..1831)
                     /locus_tag="PLY81_08450"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNF30293.1"
                     /translation="MTKTTITTNKNKSYSFFENVHEVVRLIPKGRVTTYGAIAKYLGV
                     KMSARMVGWAMNIAHTATPKVPAQRVVNRNGMLTGKIHFSTPTKMEELLKKEGINVIE
                     DKVVDFKNIFWDPSAELKW"
     gene            complement(1837..2043)
                     /locus_tag="PLY81_08455"
     CDS             complement(1837..2043)
                     /locus_tag="PLY81_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNF30294.1"
                     /translation="MSSKMIEGEDFYWENGLMVLTEKFHLSKGYCCGNGCKHCPYHYE
                     SVPEPKRTDLITLQKTSKSNPSKP"
     gene            complement(2024..2728)
                     /gene="trmB"
                     /locus_tag="PLY81_08460"
     CDS             complement(2024..2728)
                     /gene="trmB"
                     /locus_tag="PLY81_08460"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNF30295.1"
                     /translation="MGQKKLIRFNAIKTFNNVLEHPENMQGQWHKFFKNNHTITLELA
                     CGKGEYTVGLARMYVNRNFIGIDIKGNRIWRGAKTAMDEGLNNVAFLRTEIDQITQYF
                     SENEINEIWITFPDPQLRLSKAKKRLTHPKFLRLYQQILQPNGIIHLKTDSPNLYQFT
                     KTVIGLYNLELLTDYNNAYVENNIEEELKIKTHYEALDIAQSNKIHYLKFRINKNLPL
                     ALDNQLKSILHEQQND"
     gene            complement(2775..>3117)
                     /locus_tag="PLY81_08465"
     CDS             complement(2775..>3117)
                     /locus_tag="PLY81_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNF30296.1"
                     /translation="ADIFVVNKADRPDADLFVKNLRQMLAPSFSNKQGEIEVLKTIAS
                     QKKGVDILFKKIQQHATENNKEKHYLLLAEKAYHLIQQKRMKNIDKRMLKEQIEKSTE
                     NNMYKFIEQFF"
BASE COUNT          865 a          540 c          477 g         1235 t
ORIGIN      
        1 aatagcagct tctcccattg ctttacgaag ttctggggtc ataaaaggcg atggtgattc
       61 ttctactagt ttttgatggc gacgttgaat actacaatct ctttcgctca tgtggcaaaa
      121 attgccatat tggtctcctg caacttgtat ttctatatgg cgtggttctt ctacaaattt
      181 ctccatataa attccatcgt ttttaaacga tgcagcagct tctgcttttg cagtatcgta
      241 agccttctct agttcttctt ctttccaaac aactctcatt cctttaccac caccaccagc
      301 agttgctttt aaaataatgg ggtaacctac tttatttttt gctaaatctt ttgcttgttc
      361 aacactttgc aataggccgc cactaccagg tacaacaggt acgcctgcag caatcatggt
      421 ttctttggct gttattttat cacccatttt attaatcatt tcgggtgtag ggccaataaa
      481 ttttatacta tgatcggcac atatttgaga gaattttgca ttttctgcta aaaaaccata
      541 gcctggatga attgcatcgg cattggtaat ttctgcagca gccattatat gctgaatgtt
      601 taaataacta tcggcacctg ctggttttcc tatacatact gcttcgtcgg caaaacgtac
      661 atgtaaactt tccttatctg ctgttgaata aactgcaacg gttttaattc ccatttctct
      721 acaagtacgg attacacgta atgcaatttc gcctctattg gcaattaata tttttttaaa
      781 catatggagt gatgctaatt aaataaaatt aaggttccac taaaaataaa ggttggtcat
      841 actctacagg gcttgcatca tctaccaaaa cttttacaat agttcctttt acttcgcttt
      901 caatttcgtt aaacagtttc attgcttcta taatacaaac taccgagcct ggtgcaacgt
      961 ctgttcctac ttctacaaat ggaggtttat ctggagaaga ttttctgtaa aaagtgccaa
     1021 tcattgggct ttttatagta atcaaattat ctgccttggg tgcaactgct gctggtgctg
     1081 gtgcagttgg tgcagttgtt gtagcttgtg caggtgcaac tgctgctggt gctgcaacat
     1141 tataaacctg aggtgttggt gctgcaactg taactatctg ttcttctttt tgtttgatag
     1201 ttactttacc atctttatct tcaatgctaa tttctccaat attactttta ttaataattt
     1261 taatcagttc gtgaatttgt tttaaatcca taaagctagt tttaggaggt taatgtgttt
     1321 tgtttttgtt agaactttct tagttagttt gttaagcaag aaagcaaaat aggtaaaatt
     1381 tcatcaaaat tgcgtgtttt taattaaaag ttgccttttt tcttaaaaaa tgatagattt
     1441 ttatgtactt gctaatttaa acaatttacc actttagctc tgccgaagga tcccaaaaaa
     1501 tatttttaaa gtcaactact ttatcctcta taacatttat tccttctttt tttaataatt
     1561 cttccatttt ggttggtgta gaaaagtgta ttttgcctgt tagcattccg tttctattta
     1621 caactctttg tgcaggtact ttaggtgtag cagtatgtgc tatattcatt gcccaaccta
     1681 ccattcttgc actcattttt acacctaaat attttgcaat tgccccatag gttgttactc
     1741 tgcctttggg tattaaacgt actacttcgt gtacattttc aaaaaatgaa taggatttgt
     1801 ttttatttgt tgttatagta gtttttgtca agttgcttat ggtttagatg gattactctt
     1861 agaagtcttt tgtaaagtaa ttaaatcggt tctctttggt tctggaacac tttcataatg
     1921 ataggggcag tgtttacaac cattaccaca gcaataccct ttgcttaaat gaaacttttc
     1981 tgttaaaacc attaatccat tttcccaata aaaatcttct ccttcaatca ttttgctgct
     2041 catgtaaaat agattttaat tggttgtcta atgcaagtgg taagttttta tttattctaa
     2101 attttaaata atgaatttta ttgctttgtg caatatctaa agcctcataa tgtgttttaa
     2161 ttttcaattc ttcttcaata ttattttcta cataagcatt attataatct gtaagcagtt
     2221 ctaaattata taaaccaatt actgtttttg taaattgata aaggtttggg ctatctgttt
     2281 ttaaatgtat aatgccattg ggttgtaaaa tttgctgata taatcttaaa aacttaggat
     2341 gcgttagccg tttttttgcc ttgcttaaac gaagttgagg atctggaaat gtaatccaaa
     2401 tctcattgat ttcattttca gaaaagtatt gcgttatttg gtcaatttcg gttcgtaaaa
     2461 aagctacatt gttaaggcct tcatccatag ctgtttttgc cccacgccaa attctatttc
     2521 ctttaatatc tattcctata aaatttctat taacatacat tcttgctaag ccaacagtgt
     2581 attctccttt accacatgcc aactctagtg ttatagtatg gttgttttta aaaaatttat
     2641 gccattgccc ttgcatattt tctggatgtt ccaaaacatt gttgaaagtt ttaatagcat
     2701 taaaacgaat gagttttttt tgtcccatag aattggcgaa gatataatta agaatttcta
     2761 gtaatggctt acagctaaaa aaattgttca ataaatttat acatattatt ctcagttgat
     2821 ttttctattt gttctttcag cattctctta tcaatatttt tcattctttt ttgttgaatt
     2881 aagtgatatg ctttttctgc caataacaaa tagtgtttct ctttattgtt ttctgtagcg
     2941 tgttgttgaa tttttttaaa taatatatcc actccctttt tttgagaggc aatggtttta
     3001 agtacttcaa tttctccttg cttattagaa aaagaaggag ccaacatttg tcttaagttt
     3061 tttacaaata aatctgcatc tggcctatct gctttattta caacaaaaat atcagca
//
LOCUS       DAOQHP010000271         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20700_length_3110_cov_3.208838, whole genome shotgun sequence.
ACCESSION   DAOQHP010000271 DAOQHP010000000
VERSION     DAOQHP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20700_length_3110_cov_3.208838"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLY81_08470"
     CDS             <1..1340
                     /locus_tag="PLY81_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF30297.1"
                     /translation="TIQVMANQVEEKLKTTTANLAFDDMVDITTLGFGNSLEKVINDV
                     SEIYNTDINLYNNTGTLIASTQPYIYNKQLLSEKMNPIAFETLNKNKSVRFIQSESIA
                     SFKYLSIYLPVNDSDGNTYAFINIPFLNSQAELNQEISGFLSTLMNLNAFIFLLAGAI
                     AYMATNKITASFSLIGNKMKAINFEHKNETIEWKRNDEIGILVNEYNKMVLKLEASAK
                     ALAKSEREGAWREMARQVAHEIKNPLTPMKLSIQYLQKSIETKQINTPELIKNVSKTL
                     IEQIEQLARIASDFSQFANIENTQPEVFNVSEIIASLIKLYNTREDVHFVWHEPSEDF
                     LIYADKSQISRLLKNLISNAIEAGNENKQVEIMITQLINNNNIEIAIKDNGTGIDEAL
                     KQKLFTPNFTTKSSGTGLGLAICKGIVERANGTISFTTQKNIGTTFFISLPRA"
     gene            complement(1394..>3110)
                     /locus_tag="PLY81_08475"
     CDS             complement(1394..>3110)
                     /locus_tag="PLY81_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30298.1"
                     /translation="NRAGAAAICSDETSITVVNSSGFSITSQNSMTTWTANGSNTATI
                     TWNVAGTNIAPVNTPNVTILFSADGGLTFPYTIVTSTPNDGSQDIIIPSVKTTNGRFM
                     VKGDGNIFFDINNAAITVNVSCGAVGAVISPSSNVVKEVGSVDLNLALSPAYTTAFNV
                     SGNISTSDAQTTLVQATSGLTCVQYSNQYNYDVYAFTASVSGAYVITRTGSSVFNLYV
                     NSFDPGNLCNNWITSNLQGNSLANGVSVFLTAGMPYYIVVGVYYVSGNPVVALPHNYS
                     FNMATGPGVLYNGAGIYADPGASYSYSYVIVDNATNNIKAISATANLSNSATYPVGTY
                     TVYGISYLSAHAAAINGFAGGSFAALTSAITNNPSTTCADLSRNAVTVIVKGTLPVTF
                     TDLTAKKKDNDVILNWGTLTEINSSHFEIERADNGKDFNTKIGTVTSAGNSNAIKNYS
                     LVDKAPLKGMNYYRVKQVDMDGKYSYSNIAVINFERGNAHVTLYPNPAKHTLNVEYTA
                     IRNNKISVQVIDARGSVILQSNYTATIGKNIHSLNVSSLTKGVYMLKTIDTDGNITVS
                     KFIKD"
BASE COUNT         1156 a          479 c          472 g         1003 t
ORIGIN      
        1 aaactattca ggttatggca aatcaagtag aagaaaaatt gaaaactact actgccaatt
       61 tagcgtttga tgatatggtg gatattacta cactaggttt tggaaatagt ttagaaaaag
      121 tgataaatga tgtttcagag atttataata ccgatattaa tttatacaat aatacaggca
      181 cactcattgc ctccacacag ccatacattt ataataaaca gctattaagc gaaaaaatga
      241 accctattgc atttgaaact ttaaataaaa ataaaagtgt aaggtttata caatccgagt
      301 caattgcaag ttttaaatat ttaagtattt acttacctgt aaatgattct gatggcaata
      361 cgtatgcatt tattaatatt ccatttttaa actctcaggc agaattaaac caggaaattt
      421 ctggcttttt atctacacta atgaacttaa atgcatttat atttttgtta gcaggtgcaa
      481 tagcatatat ggctacaaat aaaattactg cttcttttag tttaattggc aacaaaatga
      541 aagcaattaa ttttgagcat aaaaatgaaa ctatagaatg gaagagaaat gatgagattg
      601 gaattttagt aaatgaatat aataaaatgg ttttaaagct tgaagctagt gcaaaagcat
      661 tggctaaaag tgaaagagag ggtgcttgga gggagatggc aagacaggtt gctcatgaaa
      721 taaaaaatcc tcttacccct atgaaactca gtattcaata tttgcaaaag tctatagaaa
      781 ccaaacaaat taatactcca gagcttatta aaaatgtaag taaaacatta atagaacaaa
      841 tagaacagtt agcaagaatt gcaagtgatt tttctcagtt tgcaaatata gaaaacacac
      901 aacccgaagt atttaatgtc tcagaaataa ttgcttcatt aataaagttg tataatacaa
      961 gagaagatgt tcattttgtt tggcatgaac caagtgaaga ttttttgatt tatgcagaca
     1021 agtctcaaat aagccgttta ttaaaaaatc ttatttctaa tgcaattgaa gcaggaaatg
     1081 aaaacaagca agttgaaata atgattactc aattaataaa taacaacaat attgaaattg
     1141 caataaaaga taatggcact ggtatagatg aagcattgaa acaaaaatta tttactccaa
     1201 actttactac taaatcatct ggtacaggtt taggtttggc aatttgtaaa ggaattgttg
     1261 aaagagcaaa tggtactata tcttttacaa ctcaaaaaaa tattggtact accttcttta
     1321 ttagtttacc aagggcataa aaaaaatccg attaaaaaaa tttaatcgga ttttttagta
     1381 gttacactat tatttaatct tttataaatt tagatacagt tatgtttcca tcggtatcta
     1441 ttgttttaag catatatact ccttttgtta aagaacttac atttaaacta tgaatatttt
     1501 taccaatagt tgctgtatag ttactttgca aaataactga tcctcttgca tcaattactt
     1561 gaacacttat tttattattt ctaatagctg tatattcaac atttaatgta tgcttagcag
     1621 gattagggta taaagtaaca tgagcatttc ctctctcaaa attaattaca gctatattgc
     1681 tataactata tttgccatcc atatctactt gtttaactct ataatagttc attcctttta
     1741 aaggtgcttt atcaactaaa ctgtaatttt taattgcatt gctattacct gcagaagtta
     1801 cagttccaat tttagtatta aaatctttac cattatcagc acgttcaatt tcaaaatggc
     1861 tactattaat ttcagtaagg gttccccaat ttaagataac atcattatct tttttcttag
     1921 ctgttaaatc tgtaaatgtt acaggtaatg tgcctttaac tataacggta actgcatttc
     1981 tgcttaagtc agcacaagtt gtagatgggt tattagttat tgcagaggta agagctgcaa
     2041 agctaccacc agcaaaacca tttatagctg cagcatgtgc agataagtaa ctaattccat
     2101 aaacagtata agtacctaca gggtatgttg cagagttact taaattagca gttgcactaa
     2161 tagctttaat attattagtg gcattatcta caattacata actgtaacta tagctagcac
     2221 cagggtctgc ataaattcca gcaccattat acaaaactcc agggccagtt gccatattaa
     2281 aactatagtt atgtggtaga gcaacaacag ggttaccaga tacataatat accccaacaa
     2341 caatatagta tggcatacca gcagttaaaa atacactaac cccatttgcc aaactattac
     2401 cttgtaagtt agaagtaatc cagttgttac ataagttacc aggatcaaaa ctatttacat
     2461 acaaattaaa tacagaagaa ccagtacgtg taattacata tgcaccagat acagatgcag
     2521 taaatgcata tacatcataa ttatattgat tagaatattg tacacaagtt aaacctgagg
     2581 tagcttgaac taaggttgtt tgagcatctg atgtagaaat atttcctgaa acattaaatg
     2641 cagtagtata agcaggagat aaagctaaat tcaaatctac acttcctact tcctttacaa
     2701 catttgaaga aggtgaaatt actgcaccaa ctgcaccaca agatacattt acagttattg
     2761 cagcattatt gatatcaaag aaaatatttc catctccttt aaccataaat cttccgttag
     2821 ttgttttaac agaaggaata ataatgtcct gagaaccatc atttggtgta gaagtaacaa
     2881 tagtgtatgg aaaagttaaa cctccatcag cagaaaataa aatggtaaca tttggtgtat
     2941 taacaggtgc aatatttgta cctgcaacat tccatgtaat agtagctgtg ttagaaccat
     3001 tggctgtcca tgttgtcata gagttttgag aagtgatact gaaaccacta ctattaacaa
     3061 cagtaataga agtttcatca gaacaaattg ctgcagcacc tgccctatta
//
LOCUS       DAOQHP010000272         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_20730_length_3107_cov_3.701507, whole genome shotgun sequence.
ACCESSION   DAOQHP010000272 DAOQHP010000000
VERSION     DAOQHP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20730_length_3107_cov_3.701507"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLY81_08480"
     CDS             <1..417
                     /locus_tag="PLY81_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNF30299.1"
                     /translation="LFLGKPFLGILGIDVKSFAVGGSIVIFLLGLEMVLGHEIFKGDK
                     DAKAGTVVPIAFPVIAGSGTLTTVMSLKANFNEMVILIGILINLIVIYAALKSLTLIE
                     RILGKAGLLAVRKFFGVILLAIAVKIFSSNIGSFGK"
     gene            531..1592
                     /locus_tag="PLY81_08485"
     CDS             531..1592
                     /locus_tag="PLY81_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HNF30300.1"
                     /translation="MSSVALTYDDIQLVPAYSNISSRKNIDLTTRVTKNYGLKMPLVA
                     SCMDTVCESEMAIAIAEMGGVGCIHRFMSIEQQAIEVDIVKKALQETQVINFWNVGNA
                     DWKYTKAIPIMAATGANGDFKERALALANAGANIILIDVAHGHHENVKQAIATLKNSL
                     PKYVDIIAGNIASAQAAKDLEAWQADGLRVGVGGGSLCTTRLKTGFGVPNVSCLQEII
                     AVTALPVMADGGIRTSGDIAKALALGSSCVMLGSLIAGTKEAPGKIIEKPTGLYKRYR
                     GAASLETKMVHNQEQRNVEGESTVIPFKGGAKFVIEGLLDGLKSAFSYGGASNIRDFK
                     PNYVQVTHAGQTEAKPHLL"
     gene            1657..2208
                     /locus_tag="PLY81_08490"
     CDS             1657..2208
                     /locus_tag="PLY81_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNF30301.1"
                     /translation="MSQTNQQYDLIINSCKDVFIKKATDYGTAWRVLRLISVVDQIFI
                     KALRIRTIQDLKQQKVKDNITDEFKGIINYGVIGLIQLELKESKIEELATTYVQTLYS
                     KQITLSKDTMLNKNHDYGEAWRDMSQESFTDLILMKLQRIKQILANDGKTIISEGIDA
                     NFVDIINYSVFALILISEKETNH"
     gene            2318..>3107
                     /gene="mnmA"
                     /locus_tag="PLY81_08495"
     CDS             2318..>3107
                     /gene="mnmA"
                     /locus_tag="PLY81_08495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNF30302.1"
                     /translation="MSKKGKVLVAMSGGIDSTVCALMLHHEGYEVIGITMKTWDYATS
                     VSTNSKKETGCCNVDSFNDARMAAVHHGFPHFILDIRDEFGDFVVENFVEEYLAGRTP
                     NPCVMCNTHIKWKALLKRADALDCDFIATGHYAQIQQHNNNRYYISKGLDETKDQSYV
                     LWGLGQELLSRTIMPLGKYHKTEIRQMANDMGYPELAKKSESYEICFVPDNDYRGFLK
                     RKVDGLEQKVAGGWFIDKTGKKLGKHKGYPFYTIGQRKGLDIALG"
BASE COUNT         1108 a          418 c          603 g          978 t
ORIGIN      
        1 ttatttttag gaaaaccttt tttaggtatt ttaggaattg atgtaaaaag ttttgctgtt
       61 ggaggatcta tagttatttt tttactgggc ttagaaatgg ttttaggcca cgaaattttt
      121 aaaggagata aagatgctaa agctggtaca gttgtaccca ttgcttttcc tgtaattgca
      181 ggtagtggca cacttactac tgtaatgagc ttaaaggcca actttaacga aatggttatt
      241 ttaattggta ttttaattaa cctaatagtt atttatgctg ccttaaaatc attaacatta
      301 atagaaagaa ttttaggcaa agctggctta ttggctgtgc gtaaattttt tggagtaatt
      361 ttattagcaa tcgctgtaaa aatatttagc tcaaatattg ggtcatttgg taaataattt
      421 ttaaagtaat tcacttttaa tacacaattt tggtaagcca tttttatagc tgtagtatta
      481 tgtttacttt gcttgattga aagtaaatta ttaatacaat aaattggctt atgagttctg
      541 ttgctttaac ttatgatgat atacagttag tacctgctta ttcaaacata tcttctcgaa
      601 aaaatattga tttaaccaca agggttacca aaaattatgg tttaaaaatg ccattggttg
      661 caagttgtat ggatacagtt tgcgaaagtg aaatggctat tgcaattgcc gaaatgggtg
      721 gtgttggttg catacacagg tttatgagta tagaacaaca agccatagaa gtggatatag
      781 ttaaaaaagc tttacaagaa actcaagtaa taaacttttg gaatgtaggc aacgccgact
      841 ggaagtacac aaaagctatt cctataatgg ctgcaacagg tgctaatggt gattttaaag
      901 aacgtgcctt ggcattagcc aatgcagggg caaatattat tttaatagat gttgcccatg
      961 ggcatcatga aaatgtaaaa caggctatag caacattaaa aaatagttta cctaagtatg
     1021 tagatataat agctggtaat attgcatctg cacaggctgc aaaagattta gaagcatggc
     1081 aagcagatgg cttaagagtt ggtgttggtg gtggctcctt atgcactaca cgtttaaaaa
     1141 caggctttgg agtgcctaat gtaagttgct tacaagaaat tattgctgtt actgccttac
     1201 ctgttatggc agatggtggt ataagaacca gtggcgatat agctaaagca ttggcattag
     1261 gtagcagctg tgtaatgtta ggttctttaa tagctggtac aaaagaagcc ccaggtaaaa
     1321 taattgaaaa accaacaggc ttatacaaac gttatagagg agcagcctct ttagaaacta
     1381 aaatggtaca taaccaagaa caaagaaatg tagaaggaga aagtactgta atacctttta
     1441 aaggtggagc aaaatttgta atagaaggtt tgttagatgg gttaaaaagt gccttctctt
     1501 atggtggggc aagtaacatt agagatttta aaccaaatta tgttcaggta actcatgctg
     1561 gtcaaacaga agccaaacct catttgttat aaatattttt tatttcagaa ttatattatt
     1621 ttaccactaa tcttgcagca ctgaaaaaga aaataaatga gccaaactaa tcaacaatac
     1681 gatttaataa taaatagttg taaagatgtt tttattaaaa aagcaacaga ttatggaaca
     1741 gcctggagag tattaaggct gattagtgtt gtggaccaaa tttttattaa ggctttacgt
     1801 attagaacca ttcaagattt aaaacaacaa aaagtaaagg ataatattac agacgagttt
     1861 aaaggaatta ttaattatgg tgtaattggt ttaatacaat tagaacttaa agaatctaaa
     1921 attgaagaat tagccaccac ttacgtacaa actttgtata gcaagcaaat aacattgtca
     1981 aaagatacca tgctaaacaa aaaccatgat tatggagagg cttggagaga tatgagtcag
     2041 gaaagtttta cagatttaat tttaatgaag ctacaacgca taaaacaaat acttgccaat
     2101 gatggaaaaa ccatcatcag cgaaggtata gatgccaact ttgttgatat aattaactat
     2161 agtgtttttg ccctcattct tatatctgaa aaagaaacaa atcattaatt ccttccgtta
     2221 ttttattaaa aaaattgccc atctttcatt acttccttgt ttcaaaaact acattttcaa
     2281 taaacctgta attttgtagg ttcttgttta aaaaataatg agtaaaaaag gaaaagtatt
     2341 ggttgcaatg agtggtggta ttgatagtac tgtttgtgct ttaatgctac atcacgaagg
     2401 ttatgaagtt attggcatta caatgaaaac ttgggattat gctacaagtg ttagtactaa
     2461 ctctaaaaaa gaaactggtt gttgtaatgt agatagtttt aacgatgcca gaatggctgc
     2521 tgtgcatcat ggatttcctc attttatatt agatattaga gatgagtttg gcgattttgt
     2581 tgtagaaaat tttgttgaag aatatctagc tggccgtaca cctaatcctt gtgtaatgtg
     2641 caatacccat ataaagtgga aagcattatt aaaacgtgcc gacgctttag attgtgattt
     2701 tattgcaaca gggcattatg cacaaattca acagcataat aacaacaggt actatattag
     2761 taaaggctta gatgaaacta aagaccagag ttatgtacta tggggattag gccaagaact
     2821 acttagcaga acaataatgc cattaggcaa ataccataaa accgaaatta ggcaaatggc
     2881 aaacgatatg ggctatcctg aacttgctaa aaaaagtgag agctacgaaa tttgttttgt
     2941 gccagataat gattaccgag gttttttaaa aagaaaagtg gatggcttag aacaaaaagt
     3001 agcaggtgga tggtttatag ataaaacagg aaaaaaactt ggcaaacata aaggctaccc
     3061 cttttatacc attgggcaaa gaaaaggatt ggatatagca ttaggca
//
LOCUS       DAOQHP010000273         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21284_length_3056_cov_4.020993, whole genome shotgun sequence.
ACCESSION   DAOQHP010000273 DAOQHP010000000
VERSION     DAOQHP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21284_length_3056_cov_4.020993"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..1218)
                     /gene="rfbA"
                     /locus_tag="PLY81_08500"
     CDS             complement(361..1218)
                     /gene="rfbA"
                     /locus_tag="PLY81_08500"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNF30303.1"
                     /translation="MKGIILAGGSGTRLYPITKGISKQLMPIYDKPMIYYPLSVLMLG
                     GIKEVLIITTPEDSSQFQRLLGTGEELGCVFSYAVQHTPNGLAQAFIIGADFVGSDKV
                     ALVLGDNIFYGSGFSKLVQSFNNVTGAAVFAYEVNDPERYGVVEFDEKFKAISIEEKP
                     IKPKSNYAVPGLYFYDNSVIEIAKNIAPSARGEYEITDVNRVYLEKGNLQVGVMSRGT
                     AWLDTGTFDSLSDASEFVRVIEKRQSQKIGCIEEVAYRMGFINHQQLMTLANKYAKSG
                     YGEYLRKVK"
     gene            complement(1249..1797)
                     /gene="rfbC"
                     /locus_tag="PLY81_08505"
     CDS             complement(1249..1797)
                     /gene="rfbC"
                     /locus_tag="PLY81_08505"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNF30304.1"
                     /translation="MKIETTPLKDCFIIHDTIIKDSRGYFFESFNKRKFKELTNIEID
                     FVQDNQSQSSKGVLRGLHYQKDMYAQAKLVRVLKGKVLDIAVDLRKNSPTFGQHFAVE
                     LSEDNNLQFFIPRGFAHGFIVLSDETVFFYKCDNYYNKEYDTGIKFNDPTLQINWLLK
                     EEEIIVSEKDANLPLFKDAVYF"
     gene            complement(1959..>3056)
                     /locus_tag="PLY81_08510"
     CDS             complement(1959..>3056)
                     /locus_tag="PLY81_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30305.1"
                     /translation="RVSWNQGITEGGSSGSPLFNDEHRVIGQLRGGSSYCTSPTAPDY
                     YGSFDQSWTGGGTNSTRLSNWLDPNNMNLFTTNTTNIADLIPTPATGLTILGKDSICS
                     SEIYTVNVPTGTSVSFTVSVSPNSSYASYSASGNEISVTRNGSNIGTVKITATVTDDC
                     GNQYEVNRTVYLGPIPAVIMGPYDPVEHTVMGVACVGEEYYFAPYSIDPTATYTWKFI
                     PPVWSQDWEPTYLASGPYAYFTMPDTPRCYTVRVEKTNACGGTVVTQKKICVVECNAM
                     RLLASPNPATSLLTVTLIDEKEKISKQIKQENIQIEFYNLNVGIKQKEWKYNTTAQRQ
                     FTLNIADMAKGMYVVKVTKGKLQSTTKIIKD"
BASE COUNT          960 a          544 c          455 g         1097 t
ORIGIN      
        1 gggcatattt actgcacagg gtggtgcaaa atttaacatt gggtatttaa aaaataacac
       61 acaaaccaat tatcaaatag tcaattcaag agaagattat tttggtacaa tttttttata
      121 gatgatgcaa gtatagtatc tttagacagc ataaccctta caagcagata caggagcaga
      181 cacaaacgga aatgagcaaa ttgaaaaatt aattacgcaa taaaactgtt gatagttaga
      241 tgcattaata ctatttaatt tcttcttttg tgtgttatta agttaagttg tgagagttgt
      301 acaaattaaa aaacccatct aaaaagatgg gtatattatt ttatagacat tgtaatttat
      361 ttacttcact tttctcaaat actctccata gccacttttt gcatatttat tcgctaatgt
      421 cattaattgc tgatgattga taaaacccat gcgataagca acttcttcta tacatccaat
      481 tttttgactt tgtctttttt ctatcactct tacaaactct gatgcatcgc ttaatgaatc
      541 gaaagttcct gtatctaacc aagctgttcc tctgctcatt acacctacct gtaaatttcc
      601 tttttctaaa tacactctgt ttacatctgt aatttcatat tcacctcttg cacttggtgc
      661 aatatttttt gcaatttcta ttactgagtt atcataaaaa tataatcctg gaacggcata
      721 gttagacttt ggttttatag gtttttcttc aatggatatt gctttaaact tttcatcaaa
      781 ttcaactaca ccataccttt cagggtcatt aacttcataa gcaaaaactg ctgcccctgt
      841 tacattgtta aaagattgta ctaatttact aaagccagaa ccataaaaaa tattatcacc
      901 taataccaat gctactttat cgctaccaac aaaatctgca cctattataa atgcctgtgc
      961 taaaccattg ggtgtatgtt gtacagcata agaaaataca caacctaatt cttcgccagt
     1021 acctaacaaa cgttgaaatt gtgaagaatc ttcgggtgtg gtaataatta aaacctcctt
     1081 aataccacct aacattaaaa ctgataaggg ataataaatc atgggcttat cgtaaatagg
     1141 cattagttgc ttactaattc ctttggtaat agggtaaagc cttgtaccac tacctcctgc
     1201 taaaataatt cctttcattg tactattatt ttttgagcaa atttagtttt aaaaatatac
     1261 tgcatcttta aataaaggaa ggtttgcatc tttttcagaa acaataattt cttcttcctt
     1321 taataaccaa tttatttgta aagtggggtc gttaaattta atgccagtgt catattcttt
     1381 gttgtagtaa ttatcgcatt tataaaagaa aactgtctca tcactcaata caataaaacc
     1441 atgagcaaag cctcttggaa taaagaattg taaattattg tcttcggata attctactgc
     1501 aaaatgttgc ccaaatgtgg gtgagttttt tcttaaatct acagcaatat ccaatacttt
     1561 tccttttaat actcttacca atttagcttg agcatacatg tctttttggt aatgcaaacc
     1621 tcgtaaaact cctttagaag attgggattg attgtcttgt acaaaatcta tttctatatt
     1681 agttaattct ttaaattttc ttttattaaa actttcaaaa aaatagcctc tactatcttt
     1741 tataatagta tcgtgaataa taaaacaatc ttttaaaggc gtggtttcta ttttcatttt
     1801 agttaattaa ttggctttat attggttgtg ataaaattta gttttggtta ggtattttct
     1861 gttctaaata accttaaccc ttttctttaa attccactac taccaagata atatttctat
     1921 ttggcatatt gctacttctc attaaataaa tcactttctt aatcttttat aatttttgtg
     1981 gttgattgca gtttgccttt tgtaactttt accacataca tacctttagc catatctgct
     2041 atgtttaagg taaactgtcg ttgagcagtg gtattatatt tccattcttt ttgcttaata
     2101 cctacattaa gattataaaa ttctatttgt atgttttcct gctttatttg tttgctaatt
     2161 ttttcttttt catctataag ggttactgtc agcaaacttg tagcagggtt aggagaggct
     2221 aatagcctca tggcattaca ctctacaaca caaatctttt tttgtgtaac tacagtacct
     2281 ccacaggcat ttgtcttttc tactcttaca gtataacaac gaggtgtatc tggcatggta
     2341 aaataagcat aaggacccga agctaaataa gtgggttccc aatcctgaga ccatacggga
     2401 ggtataaatt tccatgtata agttgctgta gggtctatag aatagggagc aaaataatat
     2461 tcttcaccca cacaggctac gcccataaca gtatgttcta cagggtcgta aggacccata
     2521 ataacagcag gaattggtcc aagataaaca gttctgttta cttcatactg attaccacaa
     2581 tcatcagtta cagtagcagt tatttttacc gtacctatat tagatccatt gcgtgttact
     2641 gatatttcat taccacttgc actataagat gcatagcttg aatttgggga aacggaaaca
     2701 gtaaaactta cagatgtacc tgttggcaca tttactgtat atatttctga tgaacaaata
     2761 ctgtctttac ccaatatagt caaccctgta gcaggtgtgg ggattaaatc tgcaatattg
     2821 gttgtatttg tagtaaataa gttcatatta ttgggatcaa gccagttgct tagccttgtg
     2881 gagtttgtac caccacctgt ccatgattgg tcaaagctac cataatagtc aggtgctgtt
     2941 ggagaagtac aatatgatga acctccacgt agttgaccaa tcactctgtg ctcgtcatta
     3001 aaaagtggtg agccagaaga gcctccttca gtaattccct gattccaact tacccg
//
LOCUS       DAOQHP010000274         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21323_length_3052_cov_4.453453, whole genome shotgun sequence.
ACCESSION   DAOQHP010000274 DAOQHP010000000
VERSION     DAOQHP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21323_length_3052_cov_4.453453"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="PLY81_08515"
     CDS             <1..1637
                     /locus_tag="PLY81_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF30306.1"
                     /translation="TITPVLNVVLNRKKAGHGWFYDKTEPFFKGMENGYKNLLQGFLK
                     VRWMAWVIVAVCVGSTIFIYSNLQSELAPLEDKSSVRFSLIGPEGASYDYMLKAGDQL
                     SDILIDSVKEKEFAFISVPGWGGSGVNSGVARIKFVEPEFREKSQSQLAKEITQKLAK
                     INTIRVFPIEEQTISVGLGSRGSLPVQFILQNLDFEKIKEVIPKFLEEARKDKTFQNV
                     DVNLKFNKPELHLTIDRLKAKDLGLSTTDIAEVIQSAFSGRRLAYYIMNGKQYEVISQ
                     VEYSDRQAPKDISKLYVRNNKGENIPLSAVTKMEESSSPPTLYHYNRFKAATISASLA
                     EGKTIGDGVKAMQAISDKLLDETFQTELSGPSRDFAESSSNTSFAFILALVLIYLVLA
                     AQFESFRDPFTIMITVPLALTGALLSLYLFNQTINIFSQIGMIMLIGLVTKNGILIVE
                     FANQKREFGMPKKQAVLEAAQQRLRPILMTSLATALGALPIALSLGAASTSRIPLGIV
                     IVGGLLFSLILTLFVIPAVYTFISGKHAAKQTNITE"
     gene            1664..2956
                     /locus_tag="PLY81_08520"
     CDS             1664..2956
                     /locus_tag="PLY81_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF30307.1"
                     /translation="MRNFFNLLLILITSSTYAQQISLPDAINIALKNNFDIQIAKNTV
                     TINSINNHIGIAGGLPTVSATISDQESMIGINQKLNTGVTINRSGATSNNLTANITGT
                     MLLYNGNRVIATKNKLAELQKQSEQLLNAKIQNTIADVMVKYYDIVKQQSYIKTLELA
                     IELSRKQLELVEAQKAVGMANNADLFQTEIALNTRIQDLKLQQLIINQAKTDFLNLLN
                     LNPDSLIVINDTIINDQHILLDDVLNGIHQNPLIISLEQQIKINQLVEKETAALSIPS
                     VRTNAGFNYGRNQASAGQLLLNQSYGPFINIGVSIPIYNGSSFKRQKQVATINTNNAK
                     LQKESSVAGFKSNTIKTYQAYAANLEMASTQQNTYNISAQLVNLVLQRYQLSQATILE
                     LQEALRSFESAGYRLTNLLYTIKLSEIELKRISGKLGL"
BASE COUNT         1151 a          456 c          496 g          949 t
ORIGIN      
        1 taactataac acctgtatta aacgtagttt taaacagaaa aaaagcaggg catggttggt
       61 tttatgataa aacagaacca ttttttaaag gaatggaaaa tgggtacaaa aacctattac
      121 aaggcttttt aaaagttcgt tggatggctt gggttatagt agcagtttgt gtaggctcaa
      181 caatattcat ttactccaat ttacaatctg aattagcacc actagaagat aaaagtagtg
      241 taagatttag tttaataggt ccagaaggtg ctagctacga ttatatgctt aaagcaggag
      301 atcagctatc agatatttta atagacagtg taaaagaaaa agaatttgct tttatttctg
      361 taccaggttg gggaggctct ggtgttaact caggtgttgc aagaattaaa tttgttgaac
      421 cagaatttag agaaaaatct caaagccaat tagcaaaaga aattacgcaa aaattagcca
      481 aaataaatac cattcgagtt tttcctattg aagagcaaac tatatctgtt ggtttaggta
      541 gcaggggctc cttaccagtg caattcattc tgcaaaattt agattttgaa aaaattaaag
      601 aggttatacc aaagttttta gaagaagcaa gaaaggacaa aacttttcaa aatgttgatg
      661 tgaacctgaa gtttaataaa cccgaattac atttaactat agatagatta aaagctaaag
      721 atttaggttt aagtacaacc gatattgcag aggtaataca aagtgcattt agtggtagaa
      781 ggttggctta ttatattatg aatggaaagc agtatgaagt aatttcgcag gttgaataca
      841 gcgataggca agcacctaaa gatatttcta agctttatgt tagaaataat aaaggagaaa
      901 atattccatt atctgcagtt acaaaaatgg aagaaagttc tagccctcca acactttatc
      961 actacaaccg ttttaaagca gcaaccattt ctgcatcgtt agcagaaggt aaaacaattg
     1021 gcgatggagt gaaagcaatg caagccattt ctgataaatt attagacgaa acctttcaaa
     1081 cagagctaag tgggccaagt agagactttg cagaaagtag cagcaatacc tcctttgctt
     1141 ttatacttgc tttggtttta atttacttag tattggcagc acagttcgaa agttttagag
     1201 acccttttac aattatgatt actgtaccct tagcactaac aggggcatta ttaagtttat
     1261 atttatttaa ccaaaccatt aacattttct ctcaaattgg aatgattatg cttattgggt
     1321 tagttactaa aaatggtatt ttaattgttg agtttgctaa ccaaaaaaga gaatttggaa
     1381 tgcctaaaaa gcaagcagtt ttagaagcag cacaacaaag attaagacca attttaatga
     1441 ctagccttgc aactgcctta ggtgcattac ccattgcatt aagcttagga gcagcaagta
     1501 caagtcgtat tcctttaggt attgtaattg ttggtgggct attgttttca cttatactta
     1561 cattgtttgt aatacctgct gtttatacat ttattagtgg taaacatgca gccaaacaaa
     1621 ctaatattac cgaataaatt ttgttacaat aagttttagc aacatgagaa attttttcaa
     1681 cttactactt atacttatca cttcttcaac ttatgcacag caaataagtt tacccgatgc
     1741 aattaatatt gcacttaaaa ataatttcga tattcaaata gcaaaaaata cagtaactat
     1801 taacagcatc aataatcata taggtattgc tggtggctta cctactgtat ctgcaaccat
     1861 ttcagatcag gaaagtatga ttggtattaa tcaaaaatta aataccggag taaccataaa
     1921 tagaagtggt gcaactagta ataatttaac tgccaacatt accggcacaa tgcttttgta
     1981 taatggcaat agggttattg ctacaaaaaa taaattagca gaattacaaa aacaaagtga
     2041 acaactttta aatgctaaaa ttcaaaatac cattgcagat gtaatggtaa aatattatga
     2101 tattgtaaaa caacaaagct atattaaaac cttagaactt gcaattgagc taagcagaaa
     2161 acaacttgaa cttgtagagg ctcaaaaagc agttggcatg gctaataatg ctgatttatt
     2221 tcaaactgaa attgcattaa atacaagaat acaagacctt aaattacagc agctaattat
     2281 taaccaggct aaaaccgatt ttcttaattt gcttaacctt aatccagata gtttaattgt
     2341 tattaatgat acaataatta atgatcaaca tattttatta gatgatgtac taaatggtat
     2401 tcatcaaaat ccattaatta tctctttaga gcaacaaata aaaattaacc aattagttga
     2461 aaaagaaaca gcagcattaa gcattccttc tgtaagaacc aatgcagggt ttaattatgg
     2521 aagaaatcaa gcttctgcag ggcaattact tttaaaccaa tcttatgggc cattcatcaa
     2581 tattggtgtt tccattccaa tatataatgg cagtagtttt aaacgtcaaa aacaagtagc
     2641 tactataaac actaataatg ctaaacttca aaaagaaagt tccgtagcag gttttaaaag
     2701 taatactatt aaaacatatc aagcttatgc agcaaattta gaaatggcaa gtacacaaca
     2761 aaatacttat aacatttcgg ctcaattagt aaatcttgtg ttacaacgtt atcaattatc
     2821 gcaagcaaca attttagagt tgcaagaggc attaagaagt tttgaaagtg ctggctatcg
     2881 tttaactaat ttactttaca ccataaaact ttcagaaatt gaattaaaaa gaattagtgg
     2941 taaattaggt ttgtaattct aaaaaaatta acctcttttt tttagcaaac tatacatttg
     3001 ataaattact gtagagaaaa ttaaaataaa cccaatataa aagctactgc ct
//
LOCUS       DAOQHP010000275         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21458_length_3041_cov_3.197254, whole genome shotgun sequence.
ACCESSION   DAOQHP010000275 DAOQHP010000000
VERSION     DAOQHP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21458_length_3041_cov_3.197254"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..886)
                     /locus_tag="PLY81_08525"
     CDS             complement(38..886)
                     /locus_tag="PLY81_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30308.1"
                     /translation="MNNVIEKYCNFFIAHFGLNIAKYRQRNYYSIFFKTNIQYITMKH
                     LFILIVVCFTSATSFANSNSINNNSDSANYYYAKAKESRSQRKVFDAEKFFQKALEFD
                     ANNDEIRIEFAEYYIEMRKYALATIQYRTILKDNPTHKIALPKYIGLCFLQRKWVEVI
                     KNGEIALTNNIKVENINFMMGKSYYEEEDYGKARKYLTTQFKQNPKHKETVILLGRVY
                     IEMSMYNDAIAMYKQAIESNPDDYEILYEIGLLYSAQNNDREAVKYFEMAADKGIKQD
                     LAFYKT"
     gene            complement(1017..2930)
                     /gene="glgB"
                     /locus_tag="PLY81_08530"
     CDS             complement(1017..2930)
                     /gene="glgB"
                     /locus_tag="PLY81_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNF30309.1"
                     /translation="MDTTKSVWNYSLFTATDIKNFKNGTHYSLYKLLGNKQIEVNNLK
                     GTYFAVWAPNATYVSVIGNFNNWKVKSHPLNVRGDSSGIWEGFIPSILRGEAYKYHIV
                     GYKGIKLDKGDPFAHFWEKRPATASICWDTYYEWKDVKWMKERKKNNALNAPWSVYEV
                     HLASWMRPEKNNENLYNSYQLITQKLVPYVQEIGFTHVEIMPVMEHPFDGSWGYQGTG
                     YFAPTSRFGSPQDFAAMIDAFHVAGIGVILDWVPSHFPFDAHGLYLFDGAHTFEYGDM
                     RKGFHPDWNSYIFNYKRPEVKSFLISSARFWCDRFHIDGLRVDAVSSMLRLDYSREEG
                     EWEPNEFGGNGNLDAIDFIKKLNETLYLDFPDIQTIAEEATDWPLISKPIYDGGLGFG
                     MKWMMGWMHDTLNYFKREPKDRLQHKNDFTFSMMYFYNENFMLPLSHDEVVHGKSPMI
                     YKMPGNTKQKFANLRLLYTYMFTHPGAKLLFMGNEFAQTTEWNFKSELDWELTDLVSH
                     GGMLYCIKKLNELYKNNPALYELQFSKEGFEWVNLNNTSQAIFCYLRKGKSTKDYMLI
                     ILNMSNQVQHNWKIEVGLKNKWKEIFNSDSKEFWGSGLVYNNEIQCVKTNKLNKKCTL
                     SLSLPALSAIILQ"
BASE COUNT          985 a          539 c          370 g         1147 t
ORIGIN      
        1 acaccttttg gaatattaaa tgacaggtat gccatgccta agttttgtaa aaagccaagt
       61 cttgttttat tcctttgtcg gctgccattt caaaatattt cacagcttct ctatcgttat
      121 tttgtgcact gtataataag cctatttcat ataaaatttc ataatcatca gggttgcttt
      181 caattgcttg tttatacatt gctattgcat cgttatacat gctcatttct atataaaccc
      241 ttcctaataa aataacagtt tctttatgtt tagggttttg tttaaattga gtagttaaat
      301 attttcttgc ctttccataa tcttcttctt cgtaataact tttacccatc ataaagttta
      361 tattctctac tttaatatta ttggtcaaag caatctctcc atttttaatt acttctaccc
      421 attttctttg taaaaagcaa agcccaatat attttggaag tgcaatttta tgtgtaggat
      481 tatcttttag aattgttcta tattgaattg tagctaatgc atacttacgc atttcaatat
      541 aatattctgc aaattcaatt cttatttcat cattattagc atcaaactct aatgcttttt
      601 gaaaaaactt ttctgcatca aaaacttttc tttgagatct tgattctttt gcttttgcat
      661 aataatagtt ggcactgtcg ctattattat taattgaatt tgaatttgca aaggatgttg
      721 cagaggtaaa acaaaccaca attaaaatga ataaatgttt catagtaatg tactgaatat
      781 tggttttaaa aaaaatacta taataatttc tttgcctgta ttttgcaata tttaagccaa
      841 aatgagcaat aaaaaaatta caatattttt ctataacgtt gttcatgata aatttatttt
      901 acaacaacca attaaaaatt aaaacaacaa tataatagat tgacaaacat aatattttac
      961 acaaacgggg gaatgatagt atgaagttat aattttttta tataaacaat aggatattat
     1021 tgtaaaatta tagccgaaag tgctggtaag cttaaagaaa gagtgcattt tttatttaac
     1081 ttatttgttt taacgcattg aatttcatta ttataaacca aaccagaacc ccaaaattcc
     1141 ttactatcgc tattaaaaat ctctttccat ttatttttta aaccaacttc tattttccaa
     1201 ttatgctgca cttgattgct catgtttaaa attattaaca tataatcttt agtactcttc
     1261 ccttttctta aataacaaaa gattgcttga gaagtattat tcaagttaac ccattcaaac
     1321 ccttctttac taaattgtaa ttcatataat gcagggttgt ttttgtatag ttcatttaat
     1381 ttttttatgc aatacaacat tccaccatgg ctaaccaaat ctgtaagttc ccaatctaat
     1441 tcactcttaa aattccattc ggttgtttga gcaaattcgt tacccataaa cagtaatttg
     1501 gcaccagggt gtgtaaacat atatgtataa agcaacctta gatttgcaaa tttttgttta
     1561 gtgtttcctg gcattttata aatcatgggg cttttgccat gtaccacttc atcatgactt
     1621 aagggtaaca taaaattttc gttataaaaa tacatcatgc taaaagtaaa atcgttttta
     1681 tgctgtaatc tatcttttgg ttctctttta aaatagttta aagtgtcatg catccatccc
     1741 atcatccatt tcattccaaa gcctaaacca ccatcataaa ttggtttact aattaaaggc
     1801 caatctgtag cttcttctgc aatagtttgt atatctggaa aatctaggta taaagtttca
     1861 tttaattttt taataaaatc aatagcatct aaatttccat tacctccaaa ctcatttggc
     1921 tcccactctc cttcttctct actataatct aatcgtaaca ttgatgaaac agcatcaacc
     1981 cttaacccat caatatgaaa tctatcgcac caaaatcttg cactacttat taaaaaactt
     2041 tttacttcgg gtcgtttata attaaaaata taactgttcc aatccggatg aaaccctttt
     2101 ctcatatcgc catattcaaa agtgtgagca ccatcaaata aatataaacc atgtgcatca
     2161 aaaggaaaat gagagggtac ccaatctaaa ataacaccaa taccagctac atgaaatgca
     2221 tctatcatag ctgcaaaatc ttgtggacta ccaaacctac ttgttggagc gaagtaacct
     2281 gtaccttggt agccccaact accatcaaaa ggatgttcca ttacaggcat aatttcaaca
     2341 tgcgtaaacc caatttcttg tacataaggt actaactttt gggttattaa ttgatagctg
     2401 ttgtataggt tttcattatt tttttcaggc ctcatccaac tggctaaatg tacttcatat
     2461 acactccatg gtgcatttaa agcattgttc ttttttcttt ctttcatcca ctttacatcc
     2521 ttccattcat aataagtatc ccagcaaata cttgctgtag caggcctttt ctcccaaaag
     2581 tgtgcaaatg gatctccttt gtctagcttt attcccttat aaccaacaat atggtattta
     2641 tatgcttcgc ctcttaatat acttggaata aaaccttccc aaataccact gctatctcct
     2701 ctaacattta atggatgaga ttttactttc cagttgttaa agttgccaat aaccgaaaca
     2761 taagtagcat taggagccca aacagcaaaa taagttccct ttaaattgtt tacttctatt
     2821 tgtttattac ctaataattt gtataaactg tagtgagtac catttttaaa attctttata
     2881 tctgttgcgg taaataatga ataattccaa acagatttag tagtatctat aaagtatttg
     2941 tcttcgtatt ttaatttact tttgggtgca tttttatgaa cggcggtttt gttttgtaga
     3001 ttaacttttt tactagaacc atataaatta ttatgttgcc t
//
LOCUS       DAOQHP010000276         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21563_length_3032_cov_3.680215, whole genome shotgun sequence.
ACCESSION   DAOQHP010000276 DAOQHP010000000
VERSION     DAOQHP010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21563_length_3032_cov_3.680215"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLY81_08535"
     CDS             <1..354
                     /locus_tag="PLY81_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNF30310.1"
                     /translation="DPLFKSYVFVYTNEEERVEILRTEGVLNFVKYLKEPAVIKDEEI
                     NLIKRFLAETDAKISIISDEGFKPDTLVKVSFGVFMNNVGKVVRGSKKKVYVQLQSLG
                     QLMVVEFPSGYLTPL"
     gene            364..1350
                     /locus_tag="PLY81_08540"
     CDS             364..1350
                     /locus_tag="PLY81_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF30311.1"
                     /translation="MNILLIGLGSIGKRHLKNILSIGYKNVTVVSSKNSLPEPFTALP
                     VFKTIADATQNIQFTTAVICVPTAQHRAALLECLQAGIEHIYLEKPVSNNFLGIEQIL
                     EVYSNANIYVGFDMHYDIGLQKVKQLLDENKIGKIVSANAQVGQYLPDWRPQEDYTKG
                     MSAKIDTGGGVMLDLVHEFDYLYWLFGEVDKIACFNKNSNSLNIETEDIAEVLLQFKS
                     GVIGTIHLDYLQQKLVRNCLITGSKGTIQWDLTSSKVNWITADKIENEYCYQGFERND
                     RFVNIMKDFLLQQRNSYTTNFTQGLESLKMVLAAKYSSQNNVFVKLDEFHPS"
     gene            1359..2054
                     /locus_tag="PLY81_08545"
     CDS             1359..2054
                     /locus_tag="PLY81_08545"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNF30312.1"
                     /translation="MFILGTICCRGGSKGVPGKNIKLLNGKPLIAYTIETALQTSLIN
                     DVIISTDSIDIANAAKLYGAKVPFMRPNDLATDAASKWPVFIHALEAYEKMFNVEVDY
                     LVDMDVTVPLKNSTDINGAIQKALDDSTVDVVITGYEPERNPYFNMMEILPNGFAEIV
                     KKGDKPIVRRQDAPEVYSLTPAAYVVKKSALYNYEHWSKAKCKIYPIPRERAVDIDTE
                     IDFKIVEFLMNKK"
     gene            2051..2848
                     /locus_tag="PLY81_08550"
     CDS             2051..2848
                     /locus_tag="PLY81_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF30313.1"
                     /translation="MSINLFDLSGRVAIITGGAGLLASEHAIALSNYGAKVVLADFNL
                     EKCKSAVDILQKQSINAVAKFCDVTKKESWEALLAEVLKEFGSVDILINNAGFTNQSK
                     SANFDASFENFPLEDWNAIMNVNLTGTFLGCQTVGKQMLAQGKGSIINIASLYGVVSP
                     NHNIYPGTGISQPVAYSVSKHGVVALTKYVATLWASKGVRVNALTPGGIFNNHQGLFL
                     ERFKQLNPIGRMSEKEELRGGIVYLASDASSHVVGHNLIIDGGWTAW"
BASE COUNT         1099 a          364 c          574 g          995 t
ORIGIN      
        1 gatcctttat tcaaatccta tgtttttgta tataccaacg aagaagaaag agttgaaatt
       61 ttaagaacag aaggtgtttt aaattttgta aaatatttaa aagaacctgc agtaataaaa
      121 gatgaagaaa ttaatttaat aaaacgcttc ttagcagaaa cggatgctaa aatatcaatt
      181 atttcagatg aaggctttaa gccagataca ttagtaaaag taagctttgg agtgtttatg
      241 aataatgtag gaaaagtagt aagaggtagc aagaaaaaag tatatgttca attacaaagt
      301 ttagggcaat taatggttgt ggagtttccg tcaggttatc ttacaccgct ataatatatt
      361 ataatgaata ttttattaat aggattgggc tcaataggta aaaggcattt aaaaaatatc
      421 ttatctattg gttataaaaa tgtaaccgtt gtaagtagta aaaatagttt accagagcct
      481 tttacagcgt tacctgtttt taaaacaatt gcagacgcta cccaaaatat tcaatttaca
      541 actgcagtta tttgtgtgcc aactgcacaa catagagctg cacttttaga atgtttacaa
      601 gctggtattg agcatattta tttagaaaaa ccagttagta ataatttttt aggaatagaa
      661 caaattttag aagtttattc caatgccaat atttatgtag gctttgatat gcattacgat
      721 attgggttac aaaaagtaaa acaactttta gatgaaaata aaattggtaa aattgtttct
      781 gcaaatgcac aagtagggca atatttacca gattggcgac cacaagaaga ttacaccaaa
      841 ggcatgagtg ctaaaattga tactggagga ggtgtaatgt tagatttagt gcatgaattt
      901 gattatttat actggctttt tggagaggta gataaaattg catgctttaa taaaaactct
      961 aattctttga atatagaaac agaagatatt gcagaagtgt tattacaatt taaatctggg
     1021 gttattggca ctattcattt agattattta caacaaaaat tagtacgtaa ttgtttaatt
     1081 actggtagca agggtacaat acaatgggat ttaactagta gtaaagtaaa ctggataaca
     1141 gcagataaaa tagaaaatga gtattgctat caaggctttg aacgtaatga tagatttgta
     1201 aatattatga aagattttct tttacagcaa cgtaattcat atactacaaa tttcactcaa
     1261 ggtttagaaa gtttaaaaat ggttttagct gcaaaatatt cttctcaaaa taacgttttt
     1321 gtaaaactgg atgaatttca tccttcataa tttttttaat gtttatatta ggtacaattt
     1381 gttgtagagg tggcagtaaa ggcgttccag gtaaaaatat aaaactatta aatggcaaac
     1441 cattaatagc ttatactatt gaaacagctt tacaaacttc gttaattaat gatgtaatta
     1501 tttctacaga ttctattgac attgcaaatg cagcaaagct ttatggagca aaggttccgt
     1561 ttatgagacc taatgattta gcaacagatg cagcaagtaa gtggcctgtt tttattcatg
     1621 cattagaagc ttatgaaaaa atgtttaatg tagaggtaga ttatttggta gatatggatg
     1681 taacagttcc tttaaaaaat agtactgata taaatggagc aattcaaaaa gcattagacg
     1741 attctacagt tgatgtagta attacaggtt atgagcctga gcgaaatcca tattttaata
     1801 tgatggaaat tttacccaat ggatttgctg aaattgtaaa aaaaggagat aaacccattg
     1861 ttcgtaggca agatgctcca gaagtatata gccttacacc ggctgcttat gtagtaaaaa
     1921 aatctgcctt atataattac gaacattgga gtaaagcaaa atgcaaaatt tatccaatac
     1981 caagagaaag ggctgtagat attgatacag aaattgattt taaaattgtt gaatttttaa
     2041 tgaataaaaa atgagtatta atttatttga tttatcaggt agagtagcca ttattacagg
     2101 tggtgcaggg cttttagcaa gtgaacatgc tattgcttta agcaactatg gtgctaaagt
     2161 tgttttagcc gattttaatt tggagaaatg taaatctgca gtagatattt tacaaaaaca
     2221 aagtattaat gctgttgcaa aattttgcga tgttacaaaa aaagaaagct gggaagcctt
     2281 attagcagag gttttgaaag aatttggaag tgtagatatt ttaataaata atgcaggttt
     2341 taccaaccaa agcaagagtg ccaattttga tgcaagcttc gaaaattttc ctttagaaga
     2401 ttggaatgct attatgaatg taaatcttac aggaacattt ttaggttgtc aaactgttgg
     2461 caaacaaatg ttggcacaag gcaaaggcag catcataaat attgcatcat tgtatggtgt
     2521 ggtaagtccc aatcataaca tttatcctgg tacaggtatt tctcaacctg tggcatatag
     2581 tgtaagtaag catggtgtag tagcacttac aaaatatgta gcaaccttgt gggcaagtaa
     2641 aggcgttcgt gtaaatgcac ttactccagg tggcattttt aataatcatc aaggattgtt
     2701 tttagaacgc tttaaacaat taaatcctat tggtagaatg agtgagaaag aagaattgcg
     2761 tggcggcatt gtgtatttag caagtgatgc aagcagccat gtagtaggac ataatttaat
     2821 tattgatggt ggatggacag catggtaaaa attaaaagac agttgaggtg gctaaaaaag
     2881 tcgaatacta agtgttatga tttttttcaa cttatttatg cctacttgtt cagaccaaga
     2941 aacactttca tttgctctaa agttaagttt actaataggg gtaaattgaa agttaatggt
     3001 gatttttatt ttggtgtttt gacaaataaa tt
//
LOCUS       DAOQHP010000277         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21651_length_3025_cov_2.782155, whole genome shotgun sequence.
ACCESSION   DAOQHP010000277 DAOQHP010000000
VERSION     DAOQHP010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21651_length_3025_cov_2.782155"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLY81_08555"
     CDS             <1..391
                     /locus_tag="PLY81_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNF30314.1"
                     /translation="IKTYGIDTYGSILKQYKETGTFDPAEIHSYITEGIGEDIIPENY
                     DFSLIDYIEKVTDKEAALMQRRLAKEEGIFAGNSAGAAIQGVLQIHEKFPFTKDDLVI
                     IILHDHGSRYVGKFFNDDWMKEKGFLD"
     gene            complement(461..985)
                     /locus_tag="PLY81_08560"
     CDS             complement(461..985)
                     /locus_tag="PLY81_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30315.1"
                     /translation="MVAIIAIMASCTKEGPQGPAGTNGVDGADGEDGNANVQVLLWNT
                     APTPTAGGTFHFRHGTITSQIIEEGAVLYYYLNGGNWVATPYFNSGATYGVRPAVSTG
                     VLTLTTFGGATFDISNTKSRIVLIGGSGKGTLVITRTKPWASLNEMEKAQSLYPNVDF
                     SDYNAVKKAFNLKD"
     gene            complement(1201..1752)
                     /locus_tag="PLY81_08565"
     CDS             complement(1201..1752)
                     /locus_tag="PLY81_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30316.1"
                     /translation="MEKLTKILMIAVIAIMASCSKEGPQGPAGTNGTNGVDGNANVKY
                     SDWFTPSPWVATTAYGTKDFAYTKTDAAITQSIIDNGVVLVFGKLAGYNPSIWPSTRV
                     SQLPIVVNYVSSGAQIDTWSAYLTLGTLTIDFKNNNNYYDNISITNQFRYVIIPSSSH
                     LRLSKPLQSMTYDEICSLYNIPK"
     gene            complement(1990..2517)
                     /locus_tag="PLY81_08570"
     CDS             complement(1990..2517)
                     /locus_tag="PLY81_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNF30317.1"
                     /translation="MKKLTKILMIAVIAIMASCTKEGPQGPAGTNGATGPVGPIGPEG
                     PIGNANVMYSDWFDAGAWTTSTTGSEKFYFDRPEAKITQAILDNGVVLAYTKLISDGS
                     NIRALPANTETSTFWNYILTVGNIRFTTNLASTPRPTNKFRYVIIPSTTHLRMAKPLT
                     QMSYDEVCELYNIPK"
     gene            2716..>3025
                     /locus_tag="PLY81_08575"
     CDS             2716..>3025
                     /locus_tag="PLY81_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNF30318.1"
                     /translation="MKIISLVPSITQLLFDVGLENSIVGRTKFCIHPTKKINTIPTVG
                     GTKNIDLQKINLLQPDIIFATKEENEKDQIVALKKDYKVIVFDIKNLEDNYKMIEKIG
                     K"
BASE COUNT         1054 a          452 c          448 g         1071 t
ORIGIN      
        1 tattaagact tacggaatag atacgtatgg ttctatctta aaacaatata aagagactgg
       61 tacttttgat ccagctgaaa ttcattctta cattacagaa ggaattggcg aagatataat
      121 tcctgaaaac tatgatttca gtttgattga ttatatagaa aaagtaacag acaaagaagc
      181 tgctttaatg caacgcagat tagccaaaga agaaggcatt tttgcaggaa actctgctgg
      241 tgctgctata caaggcgtgt tacaaataca cgaaaaattt ccatttacta aagatgattt
      301 agtaattatt attttgcatg accacggctc acgttatgta ggaaaattct tcaatgatga
      361 ttggatgaaa gagaaaggat ttttagatta aaaattctat ttaataaaac acaaaagcta
      421 ctcaattgag tagctttttt tataaatatt tcacaaagtt ttaatctttc aaattaaatg
      481 ctttttttac tgcattgtaa tcagagaaat caacattagg atataggctc tgagcttttt
      541 ccatttcgtt taacgaagcc catggtttag tgcgtgtaat aactaatgta ccttttccac
      601 ttccaccaat taatactatt cttgatttag tattcgaaat atcaaaggtt gctccaccaa
      661 atgtagttaa tgttaataca ccagttgaaa ctgctggacg cacaccataa gtagctccac
      721 tattaaaata aggcgttgcc acccaatttc caccattcaa ataataatac agtacagcac
      781 cttcttcaat tatttgactt gtaattgttc catgtctaaa atgaaaagta ccacctgctg
      841 taggtgttgg agcagtattc cataataaaa cttgaacatt agcattgcca tcttcgccat
      901 ctgcaccatc tacgccattg gtacctgctg gtccttgtgg accttcttta gtacacgacg
      961 ccattatggc aattattgca accattaaaa ttttttagag attcttcatg agttttaatt
     1021 taaatttatt aataatagat aaatgtaatt ctattcatta ttttctttaa aagaattgag
     1081 taattggtag aaattattta ttagttggta aaaaaagagc atttagatta attatgccac
     1141 cccaataaaa cacaaaagct actcaattga gtagcttttg ttatagaaat aattaaatgc
     1201 ttattttgga atattgtaca atgaacatat ttcgtcataa gtcatgcttt gtaaaggttt
     1261 gcttaaacgt aaatgtgaac ttgatggaat aattacatat ctaaactggt ttgtaattga
     1321 gatattatca taataattat tattgttttt aaaatctatt gtaagagttc ctagtgtcag
     1381 atatgcagac catgtatcta tttgtgcacc actgcttaca taatttacta caataggtaa
     1441 ttgtgacact cgagtgctag gccaaatgct tggattataa ccagctaatt ttccgaaaac
     1501 taatactaca ccattgtcta ttatgctttg cgtaattgct gcatcagttt tagtatatgc
     1561 aaaatctttt gtaccataag cagtagtagc tacccatgga cttggtgtaa accaatctga
     1621 atattttaca ttagcgtttc catctacgcc atttgttccg tttgttcctg ctggaccttg
     1681 tgggccttct ttgctacatg atgccataat agcaattaca gcaatcatta aaatttttgt
     1741 aagtttttcc ataagttttg ttttatttta tttttaataa tagttaaatg taaatcattc
     1801 atctatactt gtcaatttta atgagtaagt ggctcgtatt aattagtatt cggtaaaaaa
     1861 taggtatttt tcatttttaa aaattataag taaatgaaag aaaaaggatt tttagattaa
     1921 atattatact ttaataaaca caatagctac tcaattgaat agctttttta attgaaaata
     1981 gtttaatact tattttggaa tattatacaa ttcacatact tcatcgtaag acatttgtgt
     2041 taatggtttt gccatacgaa gatgagtagt agatggaatg ataacataac gaaacttatt
     2101 agtgggtcta ggtgttgatg caagatttgt agtaaatcta atattaccta cagttaagat
     2161 ataattccaa aatgtagatg tttctgtatt agcaggtaaa gctcttatat tactaccatc
     2221 tgatatcaat tttgtatatg ccaaaacaac accattatct aatatggctt gagtaatttt
     2281 agcttcaggt ctgtcaaaat agaatttttc actacctgtc gttgatgtag tccaagcgcc
     2341 agcatcaaac caatcggaat acattacatt cgcattacct attggacctt ctggtccaat
     2401 aggtccaact ggtccagtcg caccgtttgt accagctggg ccttgtggtc cttcttttgt
     2461 gcatgaagcc ataatagcaa ttacagcaat cattaaaatt tttgtaagtt ttttcataag
     2521 ttttgttttt atttttaata ataaattttg tattgcaaag attattgtat ttgaaatcta
     2581 aaacaattgg aattttagca ttttgtgaga gtaatttatt aaaaaaacaa aatatctaat
     2641 taacaatcaa ttacttaatt tatttatcag atattaaaaa atgttgtaat tttccaaaaa
     2701 ataaattatc ttttaatgaa aatcatttct ttagttccat ctattacaca actacttttt
     2761 gatgtaggat tggaaaacag tattgttggc agaactaaat tttgtattca tccaacaaaa
     2821 aaaataaata ctataccaac tgttggtggt actaaaaata ttgacttaca aaaaataaac
     2881 ttattacaac ctgatatcat tttcgctacc aaagaagaaa atgaaaaaga tcaaatagta
     2941 gcattaaaaa aagactataa agtaattgta tttgatataa aaaacttgga agacaattac
     3001 aaaatgatag aaaagattgg taaac
//
LOCUS       DAOQHP010000278         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_21944_length_3000_cov_3.521902, whole genome shotgun sequence.
ACCESSION   DAOQHP010000278 DAOQHP010000000
VERSION     DAOQHP010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21944_length_3000_cov_3.521902"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLY81_08580"
     CDS             complement(<1..585)
                     /locus_tag="PLY81_08580"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF30319.1"
                     /translation="MRKNVFNFYKKAIFTLALAMLINLHLKAQTYCSSNFTSVSFEHI
                     TNVTFAGINNTSVGTVGGPVDYTSQVATVNVGVPSTLSVTILADASEYIYAFIDWNQD
                     GDFSDSEEVYTVATSVATSGPFTLAITPPNAATPGTTRMRVMLDFAGSTPNPCKSGTY
                     GEAEDYTVNVVTSPCVAPPTAGNATSSKINACSGE"
     gene            complement(1169..1483)
                     /locus_tag="PLY81_08585"
     CDS             complement(1169..1483)
                     /locus_tag="PLY81_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNF30320.1"
                     /translation="MEQQQQNNNQLNIEITEEVAEGTYANLAIITHSHAEFVIDFVNV
                     MPGTPKSKVKSRTIFTPIHAKRFMKALAENIDRYEAAHGPIQDLEQIEIPMNFGGPNA
                     QA"
     gene            complement(1509..1925)
                     /locus_tag="PLY81_08590"
     CDS             complement(1509..1925)
                     /locus_tag="PLY81_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HNF30321.1"
                     /translation="MLQRIQSVWLLIAALLSFATLQFSFFGGNKVENGISTFINYTAK
                     ENLLVLILTVAVAVAALVSVFLYKDRRQQIKIIIATLLVNILTIVLLYFNTKKFESGT
                     IALTSVVYFAVPVFLVMALLAIRKDEKLVKSADRLR"
     gene            complement(1947..>3000)
                     /gene="glyA"
                     /locus_tag="PLY81_08595"
     CDS             complement(1947..>3000)
                     /gene="glyA"
                     /locus_tag="PLY81_08595"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNF30322.1"
                     /translation="IFGIEYANVQPHSGAQANAALMLAILKPGDDILGLDLSMGGHLT
                     HGSAVNFSGKLYKPHFYTVTREEGLIDYEMLETKAREVKPKLIICGASAYSRDIDYAR
                     IRKVADEIGAFVMADIAHPAGLIAKGLLSSPFNHCHFVTSTTHKTLRGPRGGVIMMAK
                     DFENPFGLKDPKGNIRMMSHLIDMAVFPGTQGGPLEHVIAAKAVAFGEILTNDFTLYA
                     KQVQSNAQAMAKEFVNLGYNIISGGTDNHLMLIDLRNKNISGKKAENVLGEADITANK
                     NMVPYDDKSAFVTSGIRFGVAAITTRGMKEQHMNFVVNVIDKALMNADDATILAKTKA
                     SVNEFMSQFPLYPELG"
BASE COUNT         1018 a          501 c          426 g         1055 t
ORIGIN      
        1 ctctccagaa catgcattga tttttgaact agtggcatta cctgctgtag gtggtgcaac
       61 acaaggcgat gtaacaacat ttacagtata atcttctgct tctccatagg taccactttt
      121 acatggattt ggagtggatc cggcaaagtc aagcatcacc ctcattctgg ttgtaccagg
      181 tgttgcagca tttggtggag ttattgccaa ggtaaatgga ccacttgtag caacagaagt
      241 tgcaactgta taaacctctt ctgaatcaga aaaatctcca tcttggttcc agtctataaa
      301 agcatatata tattcggaag cgtctgccaa aattgtaacg gaaagtgtac taggtacacc
      361 aacgttaacg gtagcaacct gagaagtgta atcaacagga ccacctactg tacctacaga
      421 tgtattatta attcctgcaa aagttacgtt tgtaatgtgt tcaaaagaaa cacttgtaaa
      481 attagaagaa cagtatgttt gtgcttttaa atgtaggttt attaacattg caagtgccaa
      541 tgtaaatatt gcctttttgt agaagttaaa tacatttttt cgcataagac aaattttagt
      601 ttttagtaat gaaattagca acaatattta ttaaaaatca aaaggtaatt tcaggaataa
      661 atatttaata gtttgtttta aaagaattgg agttgtcatt aaagttaatt aaagttttta
      721 aaagagaaat atcttaatta acttttaaaa aaattaacca acaagaaaag gaggtattgg
      781 aacattaacc cattcaatgg gatataggaa attatgtatt ttgaagttcc agaatttttt
      841 aaacgatatc tgtttataaa ttgctacaaa tggaacaaca ataaatatag aagtccattt
      901 ttccatgaat gagttaaaag aaactgttac gatagaaaaa aaagtgaaaa tttgttttgt
      961 taaagtaaac aaatttcctt ctacatgatg atgtaacaga aataactgtt gtagaagttt
     1021 tatcataagc agcattctat tgattaataa taaaataaca attaaatgtg tactttatta
     1081 atccattcaa caaagtaaaa gcaataatac tatttttttt taaaaaaaaa tctatatttt
     1141 aaaaaaaact acaaattgtt caaaagagtt aagcctgtgc gttcggccct ccaaaattca
     1201 ttggtatttc tatttgttca agatcctgaa ttggaccatg tgctgcctca tagcgatcaa
     1261 tattttcggc aagggctttc ataaatcttt tggcatgtat aggtgtaaaa atggttctag
     1321 attttacttt actttttggt gtaccgggca tcacatttac aaaatctatt acaaactctg
     1381 catggctatg tgttataatt gctaagttgg cataagttcc ttctgctact tcttctgtaa
     1441 tttcaatatt taattgatta ttgttttgtt gttgttgttc cataataaaa tttataataa
     1501 ttattttttt atcttaatct atctgcactc ttcaccaatt tctcgtcttt cctaattgct
     1561 aataaagcca ttactaaaaa aacgggtact gcaaaatata caacagatgt taaagcaatt
     1621 gttcctgatt caaatttctt ggtattaaaa tacaataata caattgtaag aatgttaacc
     1681 aacaaagttg caattataat ttttatttgc tgccttctat ctttatataa aaaaacagaa
     1741 actaatgctg ctacagccac agctactgta agtataagta ctaacaaatt ttcttttgct
     1801 gtataattaa taaaagtact tatgccattt tctaccttat tccctccaaa aaaagaaaat
     1861 tgtagagtgg caaaacttaa taatgctgct attaataacc aaacagattg aatacgttgt
     1921 aacataaaaa ttatttttta aaaagtttat cctaactcag gatataaagg aaactgagac
     1981 ataaactcat ttacacttgc ttttgttttt gctaaaatag tagcatcatc tgcattcatt
     2041 aaagctttgt caataacatt taccacaaag ttcatgtgtt gctctttcat tcctcttgtg
     2101 gtaattgctg caacaccaaa acgaatacca ctagtaacga atgcactttt atcatcatag
     2161 ggtaccatat ttttatttgc agtaatatct gcttctccta aaacattttc ggctttttta
     2221 ccactaatgt ttttgtttct taaatcaatt aacattaaat gattatccgt tccgccacta
     2281 ataatattat aacctaagtt aacaaattct tttgccattg cttgtgcatt actttgtact
     2341 tgttttgcat atagtgtaaa atcatttgtt aagatttctc caaatgcaac tgccttagca
     2401 gcaataacat gctctaatgg gccaccttga gtacctggga aaacagccat atctattaaa
     2461 tggctcatca ttcttatatt tcctttagga tcttttaagc caaatggatt ttcaaaatct
     2521 tttgccatca taattactcc acctcttggc cctcttaatg ttttatgtgt tgtacttgta
     2581 acaaaatgac aatgattaaa tggagaagat aataaacctt tagctattaa gcctgcagga
     2641 tgtgcaatat ctgccattac aaaagctcct atctcatcgg caacttttct tattcttgca
     2701 taatctatat ctctgctgta tgcacttgca ccacaaataa taagttttgg ttttacttct
     2761 cttgctttag tttcaagcat ttcataatct attaaacctt cttctctggt aacagtataa
     2821 aaatgtggtt tatatagttt accactaaaa tttacagcac taccatgtgt taaatgccca
     2881 cccatgctta aatctaaacc taaaatatca tcaccaggtt ttaaaattgc caacattaaa
     2941 gctgcattgg cttgtgcacc actatgaggt tgaacattgg catattcaat tccaaaaatt
//
LOCUS       DAOQHP010000279         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22051_length_2991_cov_4.878065, whole genome shotgun sequence.
ACCESSION   DAOQHP010000279 DAOQHP010000000
VERSION     DAOQHP010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22051_length_2991_cov_4.878065"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1163)
                     /locus_tag="PLY81_08600"
     CDS             complement(21..1163)
                     /locus_tag="PLY81_08600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF30323.1"
                     /translation="MYSILFISLMNSDPWGGSEVQWYATANYAIDRGDKVTCMVYEWD
                     AKKEKMELLKKRGATIIYIPNFGRAKRNLYERLSFEWVTRGKQRRFINNFIFSSFDFA
                     VVNQGGFMEVANNPWKNLYKKLPPYIVTFHNYTLNYSFKPAKAKTLTAWMLNAKCLIF
                     AAAKAISELEKQLNNSFKNVLTLASPLSIEKSLNYTPYPSLKNGKYKVIMLAHLDVNR
                     KAQDNLIKAFANDVWKKRNVILEIYGGGEHFNMLQELITKLNLQEIVFLKGNTNNVAE
                     VLNTAHLVLQITHKDAMPISVIEAMSKSRAVVVSNVGDMPVWIKDNETGWVTPDASIQ
                     SIEQGLETAWQNKTQWEQMGKNAFDFFNKNFPSSVEEVFYTTLIKK"
     gene            1208..1987
                     /locus_tag="PLY81_08605"
     CDS             1208..1987
                     /locus_tag="PLY81_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HNF30324.1"
                     /translation="MMINQQQEFFNHFNTFFNHIYVLTIERAKERQEAISKEFEGLNF
                     NFFYGFDKKNLDNMELEQNNIYSSSKAIKNSRYNKPMKLGEIACSMGHKAIYEDMIAN
                     NYSNALILEDDVSINNEPGINLENIFKQLPKNWDLVYFDYFKNDKATLVGTTKQLVYH
                     LQKKLGFLKWSHTTISNLYAKPFSANLKIAGYHDFTSAYAINLKTAKLLIEKQTPICN
                     PADHALSYIITNKLINAYITVPKIFFQKSQTNKEMYNSYVE"
     gene            2046..>2991
                     /locus_tag="PLY81_08610"
     CDS             2046..>2991
                     /locus_tag="PLY81_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNF30325.1"
                     /translation="MKIFFRLLQFSRPYHHYIPEYIVYIILYIIFGLVNFSLLAPLLD
                     VLFNNNLPVIDTAPTFSFSGAYFKDYFYFILNKYIQSNGKFGVLVYVCAIIFISILLK
                     NVFGYLGQRVLTRMRVNLLRKLRERLFFQYSNQSLSFFHKEKKGDLLSTISNDVVEIE
                     NSVVNSIQTIFRDPFIIIATFVMLFYLSYQLTLFTLIVFPISGFVISNITQKLRKKAN
                     FIQYLTGVILNIAEEAISGIRIIKGFGAEKTAQQKFDKANYSLTRTIKSIINQRELAS
                     PISEVLGVTVVLVIIVYGGHLILNGESSITASGFIAYIA"
BASE COUNT         1022 a          438 c          415 g         1116 t
ORIGIN      
        1 ttcttgaaaa gtttcataaa ttattttttg attagggttg tataaaaaac ttcttcaaca
       61 gatgatggaa aatttttatt aaaaaagtca aacgcattct tgcccatttg ttcccattgc
      121 gttttgtttt gccaagcagt ttctaaacct tgttctatgc tttgtatgct tgcatctggc
      181 gttacccagc ctgtttcatt atcttttatc catactggca tatcaccaac attgcttaca
      241 actactgctc tacttttact catagcttct attacactta ttggcattgc atctttatgg
      301 gttatttgta aaactaagtg agcagtattt aacacttctg ctacattgtt ggtattgcct
      361 tttaaaaaaa ctatttcttg taaatttaac ttagttatta attcttgtag catattaaaa
      421 tgctcccctc caccataaat ttcaagaata acatttcttt ttttccaaac atcatttgca
      481 aaggctttaa ttaaattatc ttgtgctttt ctatttacat ctaaatgtgc aagcatgatt
      541 actttatact tgccattttt taaggatggg taaggggtat agtttaaaga tttttcaata
      601 cttaaaggac tagccaatgt taatacattt ttaaaactat tattaagttg tttctctaat
      661 tctgaaatag cttttgcagc agcgaaaata aggcattttg catttagcat ccatgctgta
      721 agcgttttgg cttttgcagg cttaaaactg taatttaaag tatagttgtg aaaagttaca
      781 atataaggcg gcaatttttt atataaattt ttccaagggt tattggcaac ctccataaag
      841 ccaccttggt ttactactgc aaaatcaaaa gaagaaaaaa taaaattatt aataaacctt
      901 ctttgtttgc ctcttgtaac ccattcaaag cttagccttt catataagtt acgttttgct
      961 cttccaaaat tggggatata aataatggtt gctcctcttt ttttcaatag ctccattttt
     1021 tcttttttag catcccactc atataccata caagtaactt tatctcctct atcaattgcg
     1081 taattagctg ttgcatacca ctgcacttcg ctacctcccc atgggtctga gttcattaag
     1141 gaaataaaaa ggattgaata catgagagta acttaatttt gaaaacttat tacaaagaaa
     1201 gataattatg atgattaacc aacaacaaga attctttaac cattttaata cattctttaa
     1261 tcatatttat gttcttacaa tagaaagggc taaagaaagg caagaggcta tatcaaaaga
     1321 atttgaagga ctaaacttta atttttttta tggatttgat aaaaaaaatt tagataatat
     1381 ggaattagag caaaacaata tttatagtag ctctaaagca ataaagaata gtagatataa
     1441 caaaccaatg aaattaggag aaattgcttg cagtatggga cacaaagcta tttatgaaga
     1501 tatgatagca aacaattatt ctaatgcttt gatattagaa gacgatgttt ctataaataa
     1561 tgaacctggt attaatttag aaaatatttt taaacagcta cccaaaaatt gggacttggt
     1621 ttattttgat tacttcaaaa acgataaggc tactttagta ggtacaacaa agcaactagt
     1681 atatcattta caaaaaaaac taggattctt aaaatggtct cacacaacaa taagtaattt
     1741 atatgcaaaa cccttttctg ctaatttaaa aattgcaggc tatcatgatt ttacttcggc
     1801 atatgctatt aatttaaaaa ctgcaaaatt attaattgaa aaacaaacac ctatttgcaa
     1861 tcctgctgat catgcgttat cttatattat aactaataaa ttaataaatg catatattac
     1921 tgttccaaaa atattttttc aaaaaagcca aactaataaa gaaatgtata atagctatgt
     1981 agagtaatga gagaaaatta tctaccaact ttaatatttt aatttgcatt aaatttattt
     2041 tataaatgaa aatttttttt agactacttc agttttcaag accttatcat cattatattc
     2101 cagaatatat tgtgtatatt attttatata tcatttttgg gttggtaaac ttttctttat
     2161 tagctccatt attagatgtg ttgtttaata ataatttgcc agttatagat actgccccaa
     2221 ctttttcttt cagcggagct tattttaaag attattttta ttttatttta aataaataca
     2281 ttcaaagcaa tggcaagttt ggtgtattag tttatgtgtg tgctattatt tttatttcca
     2341 ttttattaaa aaatgttttt ggttacttag ggcagcgtgt attaactcgt atgagggtaa
     2401 acctattaag aaaactaaga gagagattat tttttcaata cagcaatcaa tctttatcat
     2461 tttttcataa agaaaaaaaa ggcgatttac tttcaactat tagtaacgat gtggtagaaa
     2521 tagagaactc ggttgtaaac tctattcaaa ccatttttag ggaccccttt attatcattg
     2581 ctacttttgt aatgttattt tatttatcgt atcaacttac tttatttact ttaatagttt
     2641 ttccaatttc tggttttgtt atttccaata ttacccaaaa actacgcaaa aaagcaaact
     2701 ttattcagta cttaacaggg gttattttaa atatagccga agaagctatt tcgggtatta
     2761 gaattattaa aggctttggt gctgaaaaaa cggcacaaca aaaatttgat aaagccaatt
     2821 actctttaac aagaacaata aaatctataa ttaaccaaag agagttggct tcgccaattt
     2881 ctgaagtgtt gggggtaaca gtagttttag taataattgt atatggtggg catttaattt
     2941 taaatggtga aagtagtatt acagcctctg gctttattgc ttatatagca t
//
LOCUS       DAOQHP010000280         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22107_length_2988_cov_3.219570, whole genome shotgun sequence.
ACCESSION   DAOQHP010000280 DAOQHP010000000
VERSION     DAOQHP010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22107_length_2988_cov_3.219570"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLY81_08615"
     CDS             <1..223
                     /locus_tag="PLY81_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30326.1"
                     /translation="EGLKRYPSQANFALIELKTGITADDFAFGLLVKSGVYTRTCSDK
                     IGLEGEFVRLASRSHDENEEIIKAIKEAL"
     gene            230..2383
                     /locus_tag="PLY81_08620"
     CDS             230..2383
                     /locus_tag="PLY81_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNF30327.1"
                     /translation="MKQLIQNFKTGELYVDELPLPALSTGMVLVENEFSLISAGTEKG
                     TVKVAQSSLLGKAKQRPDLVAQVLQNIKKEGLMATLEKVRTKLDSLKALGYSTAGVVK
                     ASLDTNGKFKVGDRVACAGQDYASHAEVVSIPQNLVAKIPDNVSFEEASFTTLGAIAL
                     QGVRQANPTLGENICVIGLGLLGQITCQLLSANGCRVFGIDVSDKMVQLAKEVSNAEA
                     YNRNNAALLTAIETFTRGNGFDKIIITAAAPTNDPIELSVQIARKKGQIIVVGAVKMD
                     VPRDPHFYRKELDLKISCSYGPGRYDNVYEEQGHDYPFAYVRWTEQRNMEAFLDLIAL
                     GKLNLKALITHTFDIDDAVRAYDLILGKVQEPFIGILLKYPENEKRKLSTFQTKNSNT
                     NHTIKASFIGAGSFAQSYLIPNLKGLPVSLDAVVTNNGINAKNVSSKFGFARASTNAN
                     EIIEDKNSNTIFIASRHDSHAQYVIKSLQENKHVFVEKPLCITYEELEAVTEVVNQKP
                     NQLLMVGFNRRFAPVSVELNKTFSKITEPKVINIRVNAGFIPLDHWTQIATIGGGRIV
                     GEMCHFIDLMQYFTNSNPVQVYAQCISSTSSQVKNDDNIAIVVTFADGSIGNLVYVAN
                     GDKAMPKERIEVFAGGCIGVINDFKDGQLYKGNKQTTIKLSGKGHKQEVESFIHAVKN
                     GTSSPIAFNSIYYTTLTTLKVIDSLNTGLPQSIIL"
     gene            2469..2942
                     /locus_tag="PLY81_08625"
     CDS             2469..2942
                     /locus_tag="PLY81_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30328.1"
                     /translation="MLRFLFILLVLLVGINTNAQSFARVEVGGYYQSFDNVKISAFEP
                     IISEQNFISEKIIFKGGFLFGRLSSILVAYPTLYNYKAPHIIVYPNPAQSNINFYISP
                     VFQVKQVTIFSTQGALVYKGTDQKNINVSHLASGVYFLHILLMDNNKVITRFVKI"
BASE COUNT         1068 a          405 c          528 g          987 t
ORIGIN      
        1 tgaaggctta aaacgttatc ctagccaagc aaattttgca ttaatagaat taaagacagg
       61 tataactgcc gatgattttg catttgggtt gttggtaaaa tctggtgttt acactcgtac
      121 ctgttccgat aaaattggat tagaaggtga gtttgtgcgt ttagcatcaa gatcgcatga
      181 cgagaatgaa gaaattatta aagcaataaa agaagcatta taaaaaacaa tgaaacaatt
      241 aatacagaat tttaagacag gagaattata tgttgatgaa cttcctttac ctgcattatc
      301 aacaggtatg gtattggtag aaaatgaatt ttcattaatc agtgcaggta cagaaaaagg
      361 aacagttaaa gtagctcaat catctttatt aggaaaagca aaacaacgcc ccgatttagt
      421 tgcacaggtt ttgcaaaata taaagaaaga aggtttaatg gcaacgcttg aaaaagtaag
      481 aaccaaatta gattctttaa aagccttagg ttacagcaca gcaggtgtag taaaagcctc
      541 tttggatact aatggtaaat ttaaagttgg agatagagta gcttgtgcag ggcaagatta
      601 tgcttctcat gccgaagtag tttctattcc tcaaaacttg gtagcaaaaa ttcctgataa
      661 tgtttctttt gaagaagcaa gttttactac tttgggtgca attgccttgc aaggagtaag
      721 gcaagctaac ccaaccttag gagaaaatat atgtgttatt ggcttgggtt tactagggca
      781 aattacttgt cagcttttat ctgctaatgg ttgtagagtt tttggtattg atgtatcaga
      841 caaaatggtt caattagcaa aagaagtaag taatgcagag gcttataata gaaataatgc
      901 agctttactt actgctatag aaacttttac cagaggtaat ggatttgata aaataattat
      961 tactgccgct gctccaacaa acgaccccat agaattatca gtacaaattg ccagaaaaaa
     1021 agggcaaata attgtggtag gtgcagttaa aatggatgta ccaagagacc ctcattttta
     1081 tagaaaagag ttagatttga aaatctcttg ttcctatgga cctggcaggt acgataatgt
     1141 atatgaagag caaggacatg attatccctt tgcttatgta agatggacag agcaaagaaa
     1201 tatggaagct tttttagatt taattgcatt aggaaaatta aatttaaaag cattaataac
     1261 ccatactttt gatattgatg atgcagttag agcctatgat ttaattttag gaaaagtaca
     1321 agaacccttt attggtattt tattaaaata tccagaaaat gagaaaagaa aactaagtac
     1381 ttttcaaact aaaaatagta atactaatca cactataaag gcttctttta ttggtgctgg
     1441 tagttttgct caaagttatt tgatacccaa tttaaaaggc ttacctgtta gcttagatgc
     1501 tgttgttacc aacaatggta ttaatgcaaa aaatgtttcc tcaaaatttg gctttgcaag
     1561 agcatctacc aatgctaatg aaattattga agataaaaat agtaatacaa ttttcattgc
     1621 ttctcgtcat gatagtcatg cacaatatgt tataaaatct ttgcaagaaa ataaacacgt
     1681 ttttgttgaa aaacctttgt gtattaccta tgaggagtta gaagctgtta cagaagtggt
     1741 aaatcaaaaa ccaaatcaac ttttaatggt tggttttaat agaagatttg caccagtaag
     1801 tgtagaattg aataaaactt tttcaaaaat aacagaacca aaagttatta atataagagt
     1861 aaatgcaggt tttattcctt tagaccattg gacacaaata gcaacaattg gaggaggcag
     1921 aattgttgga gaaatgtgtc attttattga tttgatgcaa tattttacca actcaaatcc
     1981 tgtgcaggtt tatgcacaat gtatttcaag tacaagttct caagttaaaa atgatgataa
     2041 tattgctatt gtagtaactt ttgcagacgg ctcaattgga aatttagttt atgttgctaa
     2101 tggagataaa gccatgccta aggaaagaat tgaagttttt gcaggaggct gtattggtgt
     2161 aattaacgat tttaaagatg gacaattata taaaggcaat aaacaaacaa ccattaaact
     2221 atctggtaag gggcataaac aagaagtaga gtcttttatc catgcagtaa aaaatggcac
     2281 ttcttcgcca atagctttta attctattta ttataccaca ttaactactt taaaagtaat
     2341 tgactcttta aatactggct tgccacaaag tattattttg tagttgaaaa tttatttgga
     2401 ggttgataat aatattgtgt aattttttgg agttaacgat gtatatagta agagtattaa
     2461 aattaaaaat gcttcgtttt ttatttatac ttcttgtttt acttgtgggt ataaacacca
     2521 atgcccaatc atttgcaaga gttgaagtag gcggttatta tcaaagcttt gataatgtga
     2581 aaatatctgc atttgaacca attatatctg agcaaaattt catttccgaa aaaataattt
     2641 ttaaaggagg atttttattt ggtaggcttt cttctatctt agttgcttat ccaactttat
     2701 ataattacaa agctccacat ataattgtat atcctaaccc tgcacaatct aatatcaact
     2761 tttatatctc acctgtgttt caagtaaaac aagtaacaat attttcaaca caaggtgcat
     2821 tggtttataa aggaacagac caaaaaaata tcaatgtatc tcacctagct agcggtgtgt
     2881 actttttaca tattttgctt atggataata ataaagtgat aacacggttt gtaaaaatct
     2941 aatatgatga aaaatttatt tactctatat tttctatttt ttttattt
//
LOCUS       DAOQHP010000281         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22174_length_2983_cov_3.343921, whole genome shotgun sequence.
ACCESSION   DAOQHP010000281 DAOQHP010000000
VERSION     DAOQHP010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22174_length_2983_cov_3.343921"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLY81_08630"
     CDS             <1..534
                     /locus_tag="PLY81_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNF30329.1"
                     /translation="YLEQRRKLKNLQSSNGLIHFVPFKAAAGYLNGYADANFIDELNT
                     FTLPMLTSGEYRAFEIIGDSMMPTPSGSVIVGEKVEQLDDVKNSNTYVLLSKSDGIVY
                     KRLIKNTKPKNTLTLVSDNPVYEPYQMQAQDVLEVWKAVYILQKASTVQRWEVGQLAG
                     MVNDLQTQVTNLNKKIN"
     gene            698..1627
                     /locus_tag="PLY81_08635"
     CDS             698..1627
                     /locus_tag="PLY81_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNF30330.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELATELVLLDIKEGLAEGKAQD
                     MMQTAALLGFDTVITGSTNDYTKTAGSNVVVITSGIPRKPGMTREELIGTNAGIVKGV
                     CENILKHSPNAIIIIISNPMDTMTYLALKSTGLPKNRIIGMGGILDSSRFKYQLSQHL
                     GANPNDLNAVVVGGHGDTTMIPLISKATWNSAPVTDFLSDEQQQKVVNDTMVGGATLT
                     KLIGTSAWYAPGAAGAALVEAIVRDEKKLFTCCVALDGEYNQNDICLGVPVIIGKNGW
                     EKIIDFKLSSSEQDAFNKSADAVRNMNNVLATL"
     gene            1825..2643
                     /gene="bamD"
                     /locus_tag="PLY81_08640"
     CDS             1825..2643
                     /gene="bamD"
                     /locus_tag="PLY81_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNF30331.1"
                     /translation="MKRVLLFLLFTIVLGACSNKFSKIQKSKDYQYKLKMAEQYYAKK
                     KYRFAQILFEEVFPYVKGTAEFEDTYYKFAYCYYYDKDYLNAENLFKNFVETFPNSNK
                     AEECEYMRAFCYYKQSPKVDLDQTNTNKAIGLMQAFINKHPGSARVKDASNIIDLCRE
                     KLELKRYKSAELYYNLGFYKAAAICFSNVSENFPDSKKADEYKLLVIKSYYKYAEMSI
                     PAKQEERFEMVIKECKDFTDRFQDSKYFAEAEKYKNLSTNFIKNIKNEQVKKAA"
     gene            2618..2959
                     /locus_tag="PLY81_08645"
     CDS             2618..2959
                     /locus_tag="PLY81_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNF30332.1"
                     /translation="MSKLRRQLSANTVSVIETKNLADIKAVTGNLYESISIIGKRANQ
                     INVSLKEELKNKLEEFASHTDNLEEVHENKEQIEISRAYEKMPNAAILATQEFLENKI
                     YYRNNDEDLFR"
BASE COUNT         1161 a          375 c          522 g          925 t
ORIGIN      
        1 tatttagaac aaagaagaaa attaaagaat ttacaaagtt ccaatgggtt aatacatttt
       61 gtaccattta aagcagctgc aggttactta aatggttatg cagatgcaaa ttttattgat
      121 gaattaaaca cttttacttt accaatgcta acctctggcg aatatagagc ctttgaaatt
      181 ataggcgata gcatgatgcc tacgccaagt ggcagtgtaa ttgttggcga aaaagtagaa
      241 caattagatg atgtaaaaaa cagcaatact tatgttttgt taagcaaaag tgatggtatt
      301 gtttataaaa gattaattaa aaacaccaag ccaaagaata cgcttacttt agtgagcgat
      361 aatcctgttt acgaaccata tcaaatgcaa gcacaagatg ttttagaggt ttggaaagca
      421 gtttatatat tacaaaaagc aagtactgta caaagatggg aggttgggca acttgcagga
      481 atggtaaatg atttacaaac acaggttacc aatttaaaca aaaaaataaa ttagtttttt
      541 tataggggtt agtagtaaag cctgtatcat tttggtacag gttttttgtt tctttatatt
      601 caaataacta attaaatcat ttttaagaaa aaaatagttt aacaacgtac atttgcatcc
      661 gaaaatttat tcttccataa taaaaaatat ataaagtatg aaagtaacag tagtaggtgc
      721 aggagctgta ggagcaactt gtgccgataa cattgcacgt aaagaacttg caacagaatt
      781 ggtattatta gacattaaag aaggtttagc agagggaaaa gcacaagata tgatgcaaac
      841 tgctgcatta cttgggtttg atactgtaat tacaggtagt acaaacgatt atactaaaac
      901 tgcaggcagt aatgtagtgg taataacttc tggtattcct cgtaaaccag gaatgacaag
      961 ggaagaatta attggtacca atgcaggtat tgtgaaagga gtttgtgaaa atattttaaa
     1021 acatagccct aatgccatta taattattat tagcaaccca atggatacca tgacttattt
     1081 agcattaaaa tctaccgggt tacctaaaaa tagaattatt ggtatgggtg gtattttaga
     1141 tagtagccgt tttaaatatc aattaagcca acacttagga gcaaacccta acgacttaaa
     1201 tgctgtggtg gtaggaggac atggagatac aactatgatt ccacttatta gtaaagcaac
     1261 atggaatagt gctccagtaa cagatttttt gtctgatgaa caacaacaaa aagttgtaaa
     1321 tgatacaatg gtaggcggtg ccactttaac taaactaatt ggtacaagtg catggtatgc
     1381 accaggtgct gcaggtgcag ctttagtaga agcaattgtt cgtgatgaga aaaaactatt
     1441 tacttgttgc gtagctttag atggtgaata caaccaaaat gatatttgct taggagtacc
     1501 agttattatt ggtaaaaatg gatgggaaaa aattattgat tttaaactaa gtagctctga
     1561 gcaagatgca tttaataaaa gtgcagatgc tgtaagaaat atgaataatg ttttagctac
     1621 tctctaacaa tcattttgtt ataaagttgt aatataggag caattctttt tgactaacgc
     1681 agaaaggatt gcttttttgc taaatacttg aacaaaaatt aaaactttta aaccctgttt
     1741 taggtaataa ttaactagtt taattatagt tataaataat tgcagtataa attgccgcca
     1801 aatccgtaat tttgcacact tcaaatgaaa agagtactct tatttttatt atttacaatt
     1861 gtattgggtg catgctctaa taaattttca aaaattcaaa agagcaaaga ttaccaatac
     1921 aaattaaaaa tggcagaaca gtattatgca aaaaagaaat ataggtttgc acaaatattg
     1981 tttgaagaag tttttcctta tgtaaaagga actgcagagt ttgaagacac ttattataag
     2041 tttgcttact gttattatta tgataaggat tatttaaatg ccgaaaattt atttaaaaat
     2101 tttgtagaaa cttttcctaa tagcaacaag gcagaagagt gtgagtatat gagggcattt
     2161 tgttattata aacaatcacc aaaagtagat ttagatcaaa caaacaccaa taaagcaatt
     2221 ggtttaatgc aagcatttat taataaacat cccggttctg ctagagttaa agatgcttct
     2281 aacatcattg atttgtgtag agaaaaactt gaattaaaaa gatataaaag tgccgagtta
     2341 tattataatc ttggttttta caaagcagct gcaatttgtt tttcaaatgt ttcagaaaat
     2401 tttccagatt ctaaaaaagc cgatgaatat aaattacttg taataaaatc ttactataaa
     2461 tatgccgaaa tgagcatacc tgccaaacag gaagaaagat ttgaaatggt aattaaagaa
     2521 tgtaaagact tcacagatag atttcaagat agtaaatatt ttgcagaagc cgagaagtat
     2581 aaaaatttat ccactaactt tattaaaaat ataaaaaatg agcaagttaa gaaggcagct
     2641 tagtgccaat actgtaagtg taattgaaac aaaaaattta gcagatatta aagctgttac
     2701 tggaaattta tatgaatcta tttctattat tggaaaaaga gcaaatcaaa taaatgtttc
     2761 tttaaaagaa gaacttaaaa ataagttaga agagtttgca agccataccg ataatttaga
     2821 agaagtacat gaaaataaag aacaaataga aatatccaga gcttacgaaa aaatgcctaa
     2881 tgctgcaatt ttagcaacac aagagttttt agaaaacaaa atttattata gaaacaacga
     2941 cgaagattta tttagataat tgataagtta tatatttata tta
//
LOCUS       DAOQHP010000282         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22200_length_2981_cov_3.549214, whole genome shotgun sequence.
ACCESSION   DAOQHP010000282 DAOQHP010000000
VERSION     DAOQHP010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22200_length_2981_cov_3.549214"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PLY81_08650"
     CDS             complement(<1..1402)
                     /locus_tag="PLY81_08650"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNF30333.1"
                     /translation="MSYPYQINSFEEYQQAYKKSIEQPEEFWASVAEHFTWRKKWDKV
                     LNWNFTEPTIEWFKGAKLNITENCIDRHLEKLGDKPALIWEPNNPEERVRIVTYNRLH
                     KRVCQVAQMLKNNGVKKGDRVCIYMGMVPELAYAVLGCARIGAIHSVVFGGFSAQSIS
                     DRLEDAKAEFIITCDGAYRGEKTIPLKSVIDDALIGNKTVKRIIVFTRTRTPVSMLKG
                     RDVWWEDEMDYAEEQVEKNGVVSFEPEIMDAEDPLFILYTSGSTGKPKGVVHTTAGYM
                     LFTNYSFVNIFNYKQGQIHFCTADIGWVTGHSYIVYGPLSAGATSVMFEGIPTYPDAG
                     RFWDIIDKHKINILYTAPTAIRSLMGFGTKPLENKNLSSLQVLGSVGEPINEEAWHWY
                     DEHVGKKRCPIVDTWWQTETAGILISNFAGITPSKPGWATLPMPGVQPILVDENGNEV
                     LPKENETIAGNLCIKAP"
     gene            1548..2261
                     /locus_tag="PLY81_08655"
     CDS             1548..2261
                     /locus_tag="PLY81_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30334.1"
                     /translation="MEKKFLLILFSIFLNTVSAQKIIITGLVLDSIHNSPVEFASVTN
                     ISKNKTIICNNKGFFNMVVSFGDVLSVASVNHYFDTISVQKNILKDTIIFILKPITKN
                     LGEVTVETTINKYVYDSTQRRKQFLAKVGNGKIPVFSSANSGAGLGFNITHFSKSEKI
                     KRNAYKLFDLLEKEQYINYRFSPVVVTKYTQLQNEALLAFMQAHRPTYKWLRLNVTEE
                     DIKYYINEKLKLKKTTTNN"
     gene            2347..>2981
                     /locus_tag="PLY81_08660"
     CDS             2347..>2981
                     /locus_tag="PLY81_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30335.1"
                     /translation="MKQLLIVTGLFFYLSANAQISKQATTNKTVQDKGYEIPITLSPY
                     KNTWIYLGSYFGKGKTLSDSAWLNEESKGVFKGKEKLTGGIYFIVSPSKNFIEFELLM
                     DNEQHFSITADTANRDKPTIKGSLENSLFFTYSKFSAEKGNHIQTLRGKYAAAKTKSD
                     STKCREEAIKEEKVLQAYREKIIKENPNSLLATLFHVMKKPEAPAIPIING"
BASE COUNT         1046 a          540 c          419 g          976 t
ORIGIN      
        1 aaggtgcttt tatacaaagg tttcctgcta tggtttcatt ttctttcggt aaaacttcat
       61 tgccgttttc gtcaactaaa ataggttgta caccaggcat aggcaatgta gcccaacctg
      121 gtttagatgg tgtaatgcca gcaaagtttg aaattaaaat acctgcagtt tctgtttgcc
      181 accaagtatc tacaattggg caacgttttt ttccaacatg ctcatcgtac caatgccatg
      241 cttcttcatt aataggttca ccaaccgaac ctaatacttg taaactactt aaatttttat
      301 tttctaatgg ctttgttcca aaacccatta aacttcttat agcagttggt gcagtatata
      361 aaatattaat tttatgttta tctataatat cccaaaatct tcctgcatct gggtaggttg
      421 gaataccttc aaacattaca cttgttgcac cagcactcaa tggcccatac acaatgtaac
      481 tatgccctgt aacccaacca atatctgcag tacaaaaatg aatttgacct tgtttgtaat
      541 taaaaatatt tacaaaagaa taattggtaa acagcatgta accagcagtg gtatgtacaa
      601 ctccttttgg tttgccagta ctgccactgg tatataagat aaataaaggg tcttctgcat
      661 ccattatttc aggctcaaag cttacaactc cgtttttttc cacttgctct tcagcataat
      721 ccatttcgtc ttcccaccaa acatctctac cttttaacat agatacaggt gtccttgttc
      781 gggtaaaaac gattattctt tttaccgttt tatttcctat taaagcatca tctattacag
      841 attttaatgg tattgttttt tctcccctat aggcaccatc acaagtaata ataaattctg
      901 ctttagcatc ttccaaccta tcagaaatgc tttgtgcact aaagccacca aaaacaacac
      961 tatgtattgc tccaattctg gcacaaccta aaacagcata agctaattct ggtaccatac
     1021 ccatgtaaat acaaactcta tcgccttttt ttacaccatt atttttaagc atttgagcaa
     1081 cctggcaaac tcttttatgt aatctattgt aagtaacaat tcttactctt tcctctgggt
     1141 tattgggctc ccaaattaag gcaggcttat cgcctaattt ttctaaatgt ctatcaatac
     1201 aattctctgt tatgttcagc tttgctccct taaaccattc tatagtaggt tctgtaaaat
     1261 tccagtttaa cactttatcc catttctttc tccaggtaaa atgctctgca actgatgccc
     1321 aaaactcttc tggctgttca atactttttt tatatgcttg ttggtattct tcaaaagaat
     1381 ttatttgata aggatatgac atattcttct ttaattaata ataaaaacta agcaataaag
     1441 ttaaggataa atgaactata caaaagaaag ttaaaagata cgggtaaagt aaataaagcg
     1501 gagccaactg tacttttaac caactgtatt tataatatat ttacataatg gaaaaaaaat
     1561 tcctccttat actattttct atttttttaa atactgtttc tgctcaaaaa ataattatta
     1621 caggcttagt gcttgatagt attcataaca gccctgttga gtttgccagt gtaacaaata
     1681 tttcaaaaaa caaaactata atttgcaata acaaaggctt tttcaatatg gtagttagct
     1741 ttggcgatgt actttctgta gcatcggtta atcattattt tgatacgatt agtgtccaaa
     1801 agaacattct taaagacacc atcattttta tattaaaacc tattacaaaa aacttaggag
     1861 aggtaacagt tgaaacaacc attaataaat atgtttatga tagcacacaa aggcgaaaac
     1921 aatttttagc caaagttggc aatggtaaaa tacctgtatt ttcaagtgct aatagtgggg
     1981 caggcttggg ttttaatatt acacatttca gcaaatctga aaaaataaaa cgcaatgcct
     2041 ataagctttt cgatttactt gaaaaagaac aatatattaa ttataggttt agccctgttg
     2101 tagttacaaa atacacccaa ttacaaaatg aagctttatt agcatttatg caagcccata
     2161 ggcctactta taaatggctt aggctaaatg taaccgagga ggatataaaa tattatataa
     2221 acgaaaaatt aaagttgaaa aaaactacta caaataacta ggcatatatt ttgttatacc
     2281 attttaacct tctttacagt ttgaattact taaatttacg cctccaaata aagaagtatc
     2341 attaaaatga agcaactact tattgtaaca ggtttatttt tttatctttc agcaaatgct
     2401 caaatctcaa aacaagcaac taccaataaa actgttcagg ataaagggta tgaaattcca
     2461 attacacttt cgccctataa aaatacctgg atttatcttg gtagttattt tggaaaaggt
     2521 aaaaccttat cagatagtgc atggttaaat gaagaaagca aaggtgtatt taagggcaaa
     2581 gaaaaactta ccggaggcat ttattttatt gtatccccat caaaaaattt tattgaattt
     2641 gaattactga tggataatga acaacacttt agcataacag ccgatactgc aaatagagat
     2701 aagccaacta ttaaaggctc tttagaaaat tcgctatttt ttacatattc aaaatttagt
     2761 gcagaaaaag gaaaccacat tcaaacactt agaggaaaat atgctgcagc aaaaactaaa
     2821 tcagactcta caaagtgcag agaagaagca attaaagaag aaaaagtgtt acaagcctac
     2881 agagaaaaaa ttataaaaga aaatcccaac tcactattgg ctactttatt tcatgtaatg
     2941 aaaaaaccag aagcacctgc tataccaatt attaatggca a
//
LOCUS       DAOQHP010000283         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22315_length_2973_cov_3.545579, whole genome shotgun sequence.
ACCESSION   DAOQHP010000283 DAOQHP010000000
VERSION     DAOQHP010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22315_length_2973_cov_3.545579"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLY81_08665"
     CDS             complement(<1..589)
                     /locus_tag="PLY81_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNF30336.1"
                     /translation="MAKNKTLKQLDLFAAFEPTPATVNEIEANLISAKQVVFANDKIG
                     VKLKSKNTTVTSDNSIIKKQTSPEIQIEKNLTTKNKRGRKSFKEMDAEIDLVNVPSDE
                     ILKKKLYYPIREVAAFFNVNASLIRVWETEFDILQPRKNRKGDRLFRVEDIKNIQVIY
                     YLLRNRKFSLDGAKKYLKENKGKLTTNQQLIQSLIQ"
     gene            665..1540
                     /locus_tag="PLY81_08670"
     CDS             665..1540
                     /locus_tag="PLY81_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF30337.1"
                     /translation="MNVLFYIACMNSIKTICVCGAGTMGSGIAQVTAQAGFKTIQFDV
                     NENMLDKSKIAIEKSLSFLVNKEKITEENKQAILQNLSFTSDIKECKADLIIEAIIEK
                     PEAKINLFNQLSIVNNTFTIYATNTSSISINEIANACSFKENVVGMHFFNPATIMKLV
                     EVVKANTTSHATVSSIVAVCKQMNKVPVVCKDAPGFIVNRVARHYYLEAMKIVEQNMA
                     TIENIDCLLESTGFKMGPFKLMDLIGMDINYSVSNIVWEALNKPERLKPSTLQQQKVL
                     AGELGRKTGKGFYKY"
     gene            1681..2841
                     /locus_tag="PLY81_08675"
     CDS             1681..2841
                     /locus_tag="PLY81_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF30338.1"
                     /translation="MLEKWFNWRTILLLTAIIIVGSSIFYSNYLAKKIAVDERSKMEQ
                     WAEAVRSNSNPAFTETNLSSKILTENSVEIPILLLTEKDSLLDFRNIDSTKILQDKNY
                     LKEKINQFKKLNQPIEWINPLDSTQKNKVFYGESKLLKEVRYFPMIQLLVAGLFVLII
                     VATQYVSYKSTQNQVWAGLAKETAHQLGTPISSLQGWVEMLKEINGNEKIVNEIDKDI
                     NRLLLISDRFSKIGSKPNMEEKNIIEQIENMIDYIKKRAGAKVFFKFDSNNETDLPVM
                     ISPPLFDWVIENLLKNALDAMDGKGNISVKIIDTASNIIIDVSDNGKGISKKNIGKVF
                     KPGFTTKKRGWGLGLTLTKRIIEHYHKGQIFVQHSEPNIGTTFRIMLPKSNV"
BASE COUNT         1116 a          379 c          449 g         1029 t
ORIGIN      
        1 gttgaataag agattgaata agttgctgat tagtggtaag tttaccttta ttttctttta
       61 aatatttttt ggcaccatct aaagagaatt ttctgttcct tagtaaataa taaataacct
      121 gaatattttt tatatcttcc actctaaata atctatcgcc ttttctattt tttcgaggtt
      181 gtaaaatatc aaactctgtt tcccaaactc ttattaaaga agcatttaca ttaaaaaatg
      241 cagcaacttc tcttattgga taatacaatt tcttttttaa tatttcatcg cttggtacat
      301 taactaaatc aatttcagca tccatttctt taaaactttt ccttcctctt ttatttttag
      361 tagtaagatt tttttctatt tgtatttccg gagaagtttg cttttttatt attgaattat
      421 cagaagttac agtagtgttt ttgcttttta atttaacacc aattttatca tttgcaaaaa
      481 ctacttgttt tgctgaaatt aagttagctt ctatttcatt tacagtagca ggtgtaggtt
      541 caaaagctgc aaataaatct aattgcttta aagttttatt ttttgccatt atgtaaaaat
      601 actaaatgct gtggttatta attaagtatg caatttaatg gtgtataaaa tgtaaacaac
      661 aaatatgaat gtattatttt atattgcatg catgaatagt ataaaaacaa tttgcgtttg
      721 tggtgctggt acaatgggta gtggaattgc acaggttact gctcaagctg gctttaaaac
      781 aattcaattt gatgtaaatg aaaacatgtt agataaaagc aaaattgcta ttgagaaaag
      841 cctgagtttt ttagttaata aagaaaaaat aaccgaagaa aacaaacagg caatcctcca
      901 gaatttatct ttcacttcag atattaagga atgtaaagca gatttaatta ttgaagcaat
      961 cattgaaaaa ccagaagcaa agattaactt atttaatcag ttatcaattg taaataacac
     1021 atttacaatt tatgccacaa atacttcttc tattagtatt aatgaaattg caaatgcatg
     1081 ttcttttaaa gaaaatgttg taggtatgca tttttttaac ccagccacca taatgaaatt
     1141 agttgaggtt gttaaagcaa acactacaag ccatgctaca gttagtagta ttgttgcagt
     1201 ttgtaaacaa atgaacaaag tacctgtagt ttgtaaagat gccccaggtt ttattgtaaa
     1261 tagggttgcc agacattatt atttagaagc aatgaaaata gtagaacaaa atatggctac
     1321 aattgaaaat atagattgtt tattagaatc aactggtttt aaaatgggcc cttttaaatt
     1381 gatggattta ataggcatgg atattaacta cagtgtaagt aatattgttt gggaagcatt
     1441 aaacaaacca gaacgtttaa aaccttctac tttacagcaa caaaaagttt tagctggaga
     1501 attgggtaga aaaacaggta agggatttta caaatactaa aaagcaaaat cttctgagag
     1561 tagtttttat acaaactata cgataaatga cttttctaac ttttcaattt tataactatt
     1621 tttattagaa cttaataaag attaaagtgg tatagtactt acaattattt atttttatct
     1681 atgttagaaa aatggtttaa ctggcgaaca attttattac ttactgcaat tattattgtt
     1741 ggcagcagta ttttttattc caactattta gctaaaaaaa ttgctgtaga tgaaagaagc
     1801 aaaatggagc agtgggcaga ggctgttaga tctaatagca accctgcttt tacagaaact
     1861 aatttatcaa gtaaaatact tactgaaaac agtgtagaaa ttccaattct tttacttaca
     1921 gaaaaagaca gcttacttga tttcaggaat attgattcaa caaaaatttt acaggataag
     1981 aattatttaa aagaaaaaat taaccaattc aaaaaattaa accaaccaat tgaatggatt
     2041 aacccattag atagcactca aaaaaataaa gtattctatg gagaaagcaa actcttgaaa
     2101 gaggttcgct actttccaat gatacaatta ttggttgcag gtttgtttgt tttaataata
     2161 gtagccacac aatatgtttc gtataaaagc acacaaaacc aggtttgggc aggcttggct
     2221 aaagaaactg cacatcagtt aggtactcca atttcaagtt tacaaggttg ggtagaaatg
     2281 ttgaaagaaa taaatggcaa tgaaaaaata gtaaatgaaa tagataaaga cattaataga
     2341 ttattactaa tatcagacag gtttagtaaa attggtagta agcccaatat ggaagaaaaa
     2401 aatattattg agcaaattga aaacatgata gattatatta agaaaagggc tggtgctaaa
     2461 gtgtttttta agtttgattc taataatgaa acagatttac ctgtaatgat ttcgccacca
     2521 ttatttgatt gggtaattga aaatttatta aaaaatgcac tagatgctat ggatggtaaa
     2581 ggcaatatat ctgtaaaaat tattgataca gcaagtaata ttattattga tgtttcggat
     2641 aatggaaaag gaattagtaa aaaaaatatt ggaaaagttt ttaaaccagg atttactacc
     2701 aaaaaaagag gatggggctt aggcctaact ttaaccaaaa gaattattga acattatcat
     2761 aaaggacaaa tatttgtaca acatagtgag cctaatattg gtactacctt tagaataatg
     2821 cttcctaaaa gtaacgttta acaaagcaat tttacatttc tattactttt atatgtaact
     2881 acaactccta tttttgtaaa aattatttta aacatgtaca aacttttact tctaattata
     2941 tttataccat ctattacact aaatgcacaa agc
//
LOCUS       DAOQHP010000284         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22345_length_2971_cov_2.678326, whole genome shotgun sequence.
ACCESSION   DAOQHP010000284 DAOQHP010000000
VERSION     DAOQHP010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22345_length_2971_cov_2.678326"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PLY81_08680"
     CDS             complement(<1..1509)
                     /locus_tag="PLY81_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNF30339.1"
                     /translation="MKRFILVLICSIYATLLFAQKNNGAIKFGDITTNDLATKIYSID
                     SQANAVVLYNYGHSKMEANSEGSFNLVNTFHQKIKILNKKAFDLANYKIYLFNNEGGN
                     EKIESFQAATYNLVNGEIITTKVEKSSLKKSKINEDSTLHEFTFSDIQEGSIIECSYK
                     IISPDVREMRDWFFQLSYPVLWSEFYFIYPNMFEFVALKKGVHKFSEETVYTTNERYD
                     IMIPQGVDAGGFDGNMPTINNKWVMQNIPAMEKQNFVTSMYNYLASIRFYISGIRLPG
                     AGFKSTIPTWIALSKGLMASENFGAEINKKPSWAKDEVKKLTAGITDNLEKAKKIYNY
                     VQSNIKVNYTSNIDISLPIKKIFQNKKGNAIQVNALLATLLSSADLPVKPILLSTREN
                     GKPSELYPIIDEFNYMVCYLEINDQKYFLDASSNKLGFGKLPLKCYNGAARLIDTTMP
                     SIINISADSLLETTKTNVYIINKEEGLGMTGSFTSSLGYSKSTEIRERLLTNN"
     gene            1601..2500
                     /gene="queG"
                     /locus_tag="PLY81_08685"
     CDS             1601..2500
                     /gene="queG"
                     /locus_tag="PLY81_08685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNF30340.1"
                     /translation="MKQTAYQLGFDFCGIAKAEYLNEDAKRLEQWLNKGMHGSMQYME
                     NYFDLRTDPQKLVPGAKSVITLMQNYYPDSKQNASVPKISKYAYGNDYHLSIRDKLRT
                     LLQIITEKIGQVNGRGFVDSAPVLEKTWANKSGLGWVGKNGILITKHSGSFFFIATLI
                     VDIELAYDNPFTKDYCGSCTKCIDNCPTEAILPQKVINASKCISYFTIELKDELIDES
                     MKGKFKNWMFGCDICQDVCPWNRFSKPTNEIAFTPIPEVLNLTTQQWEELSEESFKKI
                     FKNSAIKRSKYKGILRNLKFLKQ"
     gene            complement(2502..>2971)
                     /locus_tag="PLY81_08690"
     CDS             complement(2502..>2971)
                     /locus_tag="PLY81_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNF30341.1"
                     /translation="DDKFTLIGDAAHAVVPFFGQGMNCGFEDCRILDELMEANENNWH
                     NILEAFQQTRKPDADAIADLAIENFIEMRAKTADPKFLLQKKIEVRLHEKYPNQWIPA
                     YAQVTFSPNIRYSDALKRGQIQEKIMQQIMQIPNIEAIWDSNEVEEKILSLIP"
BASE COUNT         1022 a          467 c          414 g         1068 t
ORIGIN      
        1 attattcgtc aatagtcttt ctctaatttc tgtagatttt gaataaccca atgatgaagt
       61 aaaggaacca gtcattccaa gcccttcttc tttattgata atatatacat ttgttttagt
      121 agtttcaagc aacgagtctg cagaaatatt tataatagat ggcatagttg tatcaatcaa
      181 tcttgcagct ccgttataac attttaaagg taattttccg aaccctaatt tatttgaaga
      241 agcatctaaa aaatattttt gatcgttaat ttctaaatag caaaccatat aattaaactc
      301 atcaataatt ggatacagct cagatggttt accattttct cttgtgctta gcaatatagg
      361 cttaacaggt aaatctgctg aactgagcaa ggttgctaac aaagcattta cctgtattgc
      421 atttcctttt ttattctgaa atattttttt aattggtaat gaaatatcaa tatttgaagt
      481 gtaatttact ttaatatttg attgaacata gttgtaaatt ttttttgctt tttctaaatt
      541 atctgttata ccagcagtta gttttttaac ttcatctttt gcccaagatg gttttttatt
      601 aatctctgca ccaaaattct cacttgccat taatcctttt gataaggcta tccaagttgg
      661 tatagttgat ttaaagcctg cacctggtaa tcttatacca ctaatataaa acctgataga
      721 tgctaaataa ttatacatgg atgttacaaa attttgtttc tccattgccg gaatattttg
      781 catcacccat ttattgttta ttgtaggcat attaccatca aatccgccag catctacacc
      841 ttgtggtatc ataatatcat aacgctcatt tgtagtataa acagtttcct ctgaaaattt
      901 atgtacccct ttttttaacg caacaaattc aaacatattt gggtaaataa aataaaactc
      961 actccataat acagggtaag ataactgaaa gaaccaatcc ctcatctccc taacatcagg
     1021 cgaaataatt ttgtaactac attctataat tgaaccttcc tgaatatcag aaaaagtaaa
     1081 ttcatgtaaa gtactatctt cattaatttt tgattttttt aatgagctct tttctacttt
     1141 tgtagttata atttctccgt taactaaatt ataagttgca gcctgaaaag attctatttt
     1201 ttcgtttcct ccttcattat tgaataaata aattttataa tttgctaaat caaatgcctt
     1261 tttatttaat attttaattt tttgatgaaa agtattaact aaattaaaag atccttcgct
     1321 attagcctcc attttagagt gtccataatt gtataaaact actgcgtttg cctggctatc
     1381 tatactatat atttttgtgg ctaaatcgtt ggtagtaata tcaccaaatt ttatagcacc
     1441 attatttttt tgagcaaata ataatgtagc gtaaattgag caaattaaaa ctagaataaa
     1501 ccttttcata agttatacaa ttattatagc aaaatactga ataagctatt acatatcaaa
     1561 attcattatt atcagtaacc aaacaacata tagccaaaca ataaaacaaa ctgcttacca
     1621 attaggtttt gatttttgtg gaattgcaaa agctgaatat ttaaatgaag atgcaaaacg
     1681 tttagaacaa tggttaaata aaggcatgca tggaagtatg caatacatgg aaaattattt
     1741 tgaccttaga actgatcctc aaaaattagt gccaggtgca aaaagtgtaa ttactttaat
     1801 gcaaaattat tatcctgatt caaagcaaaa tgcatctgtt cctaaaatat ctaaatatgc
     1861 ttatggtaac gattatcatt tgagcattag agataagctt agaacattat tacaaattat
     1921 aaccgaaaaa attggtcaag taaatggccg tggttttgta gatagtgcac ctgttttaga
     1981 aaaaacctgg gctaataaaa gtggcttggg ttgggtagga aaaaatggaa tattaataac
     2041 aaaacattct ggctcctttt tttttatagc aacattaatt gtagatattg aattggctta
     2101 tgataaccct tttactaaag actattgtgg ctcatgcaca aaatgtattg ataactgccc
     2161 caccgaagca attttaccac aaaaagtaat taatgcaagt aagtgtatta gttattttac
     2221 tattgaatta aaagatgaat taattgatga aagtatgaaa ggcaaattta aaaattggat
     2281 gtttggctgc gatatttgcc aagatgtttg cccttggaat agatttagta aacccaccaa
     2341 cgaaattgca tttacaccca taccagaagt gttgaattta actacccaac aatgggaaga
     2401 attaagtgaa gaaagtttca agaaaatttt taaaaactct gcaataaagc gaagtaaata
     2461 caaaggaatt ttaaggaatt taaaattttt gaaacaataa tttagggtat taaagaaaga
     2521 attttttctt ctacttcatt actatcccaa attgcctcta tattgggtat ttgcataatt
     2581 tgttgcataa ttttttcttg aatttggcca cgttttaaag catcgctata acgaatattt
     2641 ggagaaaaag taacttgagc ataagcaggt atccattggt taggatattt ttcatgtagt
     2701 cttacttcta tttttttctg taataaaaac ttagggtcgg ctgttttagc cctcatttca
     2761 ataaagtttt caatagctaa atctgcaatt gcatctgcat ctggttttct agtttgttga
     2821 aaggcctcta atatattatg ccaattgttt tcatttgcct ccatcaactc atctaaaatt
     2881 ctacaatcct caaaaccaca attcattcct tgcccaaaaa atggcactac agcatgagct
     2941 gcatctccaa ttaatgtaaa cttatcgtct c
//
LOCUS       DAOQHP010000285         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22384_length_2967_cov_3.626374, whole genome shotgun sequence.
ACCESSION   DAOQHP010000285 DAOQHP010000000
VERSION     DAOQHP010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22384_length_2967_cov_3.626374"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLY81_08695"
     CDS             <1..735
                     /locus_tag="PLY81_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNF30342.1"
                     /translation="YLGVKIRYADLLTKPHRYNNSPVWSGLMNNGRAYSAYTYNVERL
                     VPWRTLTGRQHFYLDQEMYIAYDEHLPTFKPAPSPEVYGDLRETWKNGKAKALNYLTP
                     HGKWHIHSTYMENLRMLTLSRGIEPCWLSEVDAEELGIKDNDWVEVHNDHGVYVTRAC
                     VSSRIPPGVCVVYHVPERTVGIPKSQLRGNRRGGGHNSVTRIHLKPNYLNGGYGQFSY
                     HFNYWGPVAPQRDTHVIVKKMEKLVW"
     gene            781..2331
                     /gene="narH"
                     /locus_tag="PLY81_08700"
     CDS             781..2331
                     /gene="narH"
                     /locus_tag="PLY81_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNF30343.1"
                     /translation="MDVRSQIAMVFHLDKCIGCHTCSIACKNIWTDRKGAEYMWWNNV
                     ETKPGTGYPTKWEDQEIYKGGWEKRNDKIQLKGAGKRRGLTNIFYNPNLPVIDDYYEP
                     FTYKYLDLIESPETDDQPTARPVSLITGQPIDIKMGPNWDDDLSGSPDYARNDPNFKG
                     MTAEEQEVMFQLEKMAFFYLPRICNHCLNPACVASCPSGAIYKRGEDGVVLINQEVCR
                     AWRMCVTACPYKKSYYNWHTGKSEKCILCYPRMEAGLAPACMHSCVGRIRYLGVLLYD
                     ASKIEATASVDEKDLINSQMDMLLDPFDENVIREAKKNGVADSTIHAAQNSPIYKFVK
                     EWKLALPLHPEFRTLPMLFYVPPLLPVMATVKKTDNTEQQDKMHPIAKKWEDNWLYDT
                     STEELWGTLDQIRFPLKYIANLFSAGDEELIKEKWKKLMAVRIHRRKVTVGDISDEKV
                     ATTLRDAGMTAEEADAIYALTSLPKFEERFVIPAAHREQAIEMMDFTGDTKGDAGFGI
                     KTKPVRGI"
     gene            2364..>2967
                     /locus_tag="PLY81_08705"
     CDS             2364..>2967
                     /locus_tag="PLY81_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30344.1"
                     /translation="MEMVQNNNTSTANDLANYELLASLFLYPDNNQYKNGIEKVYNYL
                     NNSFPEAAITLQPFVEFINNNSAVEIQELYLRSFDVQAITTLDIGFVLFGEDYKRGKL
                     LVHLNEEHNKVGNQCETELSDHLPNLLKLLPKIGDDEVRNEIATLLIIPAVEKMISEF
                     SIEKIEKKDTVYKKHQKVLLDYSQNYRTIFQSTLMALFMVL"
BASE COUNT         1116 a          451 c          564 g          836 t
ORIGIN      
        1 tatttaggag taaaaattag atatgccgat ttattaacta aaccacatag atacaacaat
       61 tcacctgttt ggtcgggttt aatgaataat ggaagagcat actctgctta cacctataat
      121 gtggaaaggc tagtaccttg gagaacatta actggtcgtc agcattttta tttagaccaa
      181 gaaatgtaca tagcgtacga tgaacattta ccaacattta aacctgcacc aagcccagaa
      241 gtgtatggag atttgagaga aacctggaaa aatggaaaag caaaagcatt aaattattta
      301 acaccacatg gtaaatggca tattcactct acctacatgg aaaacttgag aatgttaacc
      361 ttatcaagag gaatagaacc ttgttggtta agtgaggtag atgctgaaga actaggcatt
      421 aaagataacg actgggtaga agttcataac gaccatggtg tgtatgtaac aagagcctgc
      481 gtaagctcaa gaattccacc aggtgtatgt gttgtatatc atgtgccaga aagaacggta
      541 ggtattccaa aatcgcaact aagaggcaat agaagaggag gagggcataa tagtgttaca
      601 cgtattcact taaaaccaaa ttatttaaat ggcggttatg gccagttttc ctatcatttc
      661 aattattggg ggcctgtggc accacaaaga gatacccatg taattgttaa aaaaatggaa
      721 aaactagttt ggtaaaaata atagcagtat ttaaaattca tcattcaaaa ctataaactc
      781 atggatgtac gatcacaaat agctatggta tttcacttag ataaatgtat cgggtgtcat
      841 acatgttcta ttgcctgtaa aaatatttgg acagatagaa aaggtgcaga atatatgtgg
      901 tggaataatg tagaaaccaa accaggtaca ggctatccaa ctaaatggga agatcaggaa
      961 atttataaag gaggctggga aaaaagaaac gacaaaatac aattaaaggg tgcaggcaaa
     1021 agacgtgggc taaccaacat tttttataac cccaacttac ctgttattga tgattattat
     1081 gaacctttta cgtataaata tttagaccta atagagtctc cagaaacaga tgaccaacca
     1141 acagctcgcc ctgtttcatt aattactggt caaccaatag atattaaaat gggacctaac
     1201 tgggatgatg acttaagtgg ctctccagac tatgccagaa atgatccaaa ctttaaaggc
     1261 atgactgcag aagaacaaga agtaatgttt cagttagaaa aaatggcctt cttttatttg
     1321 ccaagaattt gtaaccattg cctaaatcct gcctgtgttg catcttgccc atctggagca
     1381 atttacaaac gtggcgaaga tggagtagta ttaatcaatc aagaagtgtg cagagcttgg
     1441 agaatgtgtg ttacggcatg cccttataaa aaatcttatt acaactggca tacaggaaaa
     1501 tcagaaaaat gtattttatg ttatccaaga atggaggcag gtttagctcc agcatgtatg
     1561 cactcctgcg ttggaagaat tagatattta ggagttttac tttacgatgc ttctaaaata
     1621 gaagctacag caagtgtaga tgaaaaagat ttaatcaata gccaaatgga tatgctttta
     1681 gatccatttg atgaaaatgt aattagagaa gcaaagaaaa acggcgtagc agattctacc
     1741 attcatgcag cacaaaactc accaatatac aaatttgtaa aagaatggaa attggctttg
     1801 ccattgcacc cagagtttag aacattacca atgttatttt atgttccacc attgttacct
     1861 gtaatggcaa ctgttaaaaa aacagataat acagaacaac aagataaaat gcatccaatt
     1921 gcaaaaaaat gggaagataa ttggttgtat gatacaagta ccgaagagct ttggggtaca
     1981 ttagatcaaa ttcgttttcc attaaaatat attgccaatt tatttagtgc tggtgatgaa
     2041 gaactaataa aagaaaaatg gaaaaaatta atggccgttc gtattcatag aagaaaagta
     2101 acggttggag atattagtga tgaaaaagtt gcaacaacac ttagagatgc aggcatgact
     2161 gctgaagaag cagatgctat atatgcttta acatcattac caaaatttga agaaaggttt
     2221 gttattccag cagctcatag agaacaagca atagaaatga tggattttac aggagacact
     2281 aaaggcgatg caggatttgg tattaaaaca aaaccagtac gaggcattta aaaataattt
     2341 aagagtaaat aaaatatgta gctatggaaa tggtacaaaa taataatacc tctactgcca
     2401 acgatttggc aaactacgag ttgttagcat ccttattttt atatccagat aataaccagt
     2461 ataagaatgg aattgaaaaa gtatataatt atttaaacaa tagttttccg gaagctgcaa
     2521 taaccttaca accttttgtt gaatttatta ataacaattc agctgtagaa attcaagaac
     2581 tttatttacg atcttttgat gttcaggcta tcactacttt agatattggg tttgttttat
     2641 ttggcgaaga ttataaaaga ggaaaattgc ttgttcactt aaacgaagaa cataataagg
     2701 tgggcaacca atgcgaaaca gaattatccg accatcttcc taatttatta aaactattac
     2761 ctaaaatagg agatgatgaa gtgagaaatg aaatagcaac cttgctaatt atacctgcag
     2821 ttgaaaaaat gattagtgaa ttttcgatag agaaaataga aaagaaagat acggtatata
     2881 aaaaacacca aaaagtattg ctagactatt ctcaaaatta tcgaacaata tttcaatcca
     2941 cattaatggc tttgtttatg gttttac
//
LOCUS       DAOQHP010000286         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22521_length_2956_cov_4.183385, whole genome shotgun sequence.
ACCESSION   DAOQHP010000286 DAOQHP010000000
VERSION     DAOQHP010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22521_length_2956_cov_4.183385"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLY81_08710"
     CDS             complement(<1..432)
                     /locus_tag="PLY81_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNF30345.1"
                     /translation="MAYSNKKFTPIQAYNKIQYYCSYQERSHFEVKEKLYGYGLNKLE
                     VETIIIQLIEENFLNEERFAIQFAGGKFRQKKWGKNKIVYELKNKRVSSRNIQIGINS
                     IDSLQYQNTLHKLALQKWNSLKKDSIIAKKVKTTKYLLQKGF"
     gene            645..1760
                     /gene="dnaN"
                     /locus_tag="PLY81_08715"
     CDS             645..1760
                     /gene="dnaN"
                     /locus_tag="PLY81_08715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNF30346.1"
                     /translation="MKFIVSSSSLLKHLQQINGVISANTVLPILEDFLFEIQDKKLSI
                     TATDLETVMRVQLDVESKTNGKVCIPAKILMDSLKNIADQPLTFSIDKNFAVEITSDN
                     GKYKVMGENPDNFPKEPVSDDATEFTMTSSALVTGINKSLFAVSTDDLRPAMTGVFFE
                     LKTDGVQFVATDAHRLVRYKRTDTKASKNDSFIVPKKPLTLLKGALPNNDDELSISYN
                     ASHLFVVHGNIQMTCRLIDARFPDYKVVIPAENPYHLTVNKDDFQSALRRVSVFSNKS
                     TNQVALSISSNELQMAAQDVDFSFEGNERMGCSYNGEDMQIAFNAKFLIEMLSAADTN
                     EVRLELSTPSKAGLIKPSEQEDGEDLLMLVMPLMLNN"
     gene            complement(1820..>2956)
                     /locus_tag="PLY81_08720"
     CDS             complement(1820..>2956)
                     /locus_tag="PLY81_08720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF30347.1"
                     /translation="NVTFILMKNWVDLFIRQGDKQVIIKEIDGLRFIAIIAVVLSHFS
                     LQIIKQSNLPVDFEYANGVALFLELGGYGVRIFFCISAFILSIQFIKYYLYNSEKVSL
                     KKYYFKRLKRLEIPYLLVLIILLLFRIFVQGEFWKDEMPHFFSSVFYSHNIIYDRRST
                     INPVAWTLEIEIQFYLLLPFLMQIFLIKNFVKRRVLLVGCVLLFALLHSIFYHYITVA
                     HLTYSIVPYLPIFLLGILFADIYLSNKILLESKFFLWDVLGIIGFLIILYFAGYASFY
                     KYIIEMFGYVLLFTGIFKGRILNAIFTKKIWMAIGCMCYSIYLLHYALIAFIMQHITI
                     SIFKKGYYNNLVLQGIIVLPLVLFICSIFYLIIEKPLTTKNRNI"
BASE COUNT         1112 a          419 c          398 g         1027 t
ORIGIN      
        1 aaatcctttt tgtaataaat actttgttgt ttttactttt ttggcaataa ttgaatcttt
       61 tttcagactg ttccattttt gtaaagctaa tttgtgtaag gtgttttgat attgtaaact
      121 atcaattgaa ttaattccaa tttgaatatt tctactactt acccttttgt ttttaagttc
      181 atacactatt ttatttttcc cccatttttt ttgtctgaat ttaccacctg caaattgaat
      241 ggcaaacctt tcttcattta aaaaattttc ttcaataagt tgtataataa tagtttctac
      301 ttctaactta tttaatccat agccatacag tttttctttt acttcaaagt ggctgcgttc
      361 ttgatagcta caataatatt gtattttatt gtaggcttgt ataggggtaa attttttgtt
      421 gctataagcc aagtttatcg tttttagaat ctgtaaaaaa taaaagatag aacttattta
      481 aaatattctt tatttagata agttcctaac aaaattcaca taatagccca aatgttgtga
      541 ataaaacttt acgaggaaca taatttctac aaaaattaat caatttacat taggtttgct
      601 ccaagccgaa aaaatcggat atgctatcat aaaaaatatt tattatgaaa tttattgttt
      661 catcttcgtc tttattaaag catttacaac aaataaatgg tgttataagt gccaatactg
      721 ttttaccaat tttggaagat tttttatttg aaattcagga taaaaaatta agcattactg
      781 caaccgattt agaaactgtt atgagagttc aactagatgt ggaaagtaaa acaaatggta
      841 aggtatgtat tcctgcaaaa atattaatgg attcgttaaa gaatattgca gaccaacctt
      901 taacttttag cattgataaa aattttgctg tagaaattac cagcgataat ggaaagtata
      961 aagtgatggg agaaaatcca gataattttc ctaaagaacc agtatctgat gatgctacag
     1021 aatttactat gactagttct gccttagtta caggtatcaa taaatcttta tttgcagtaa
     1081 gtaccgatga tttacgccct gcaatgactg gtgtgttttt tgaactaaaa acagatgggg
     1141 tacagtttgt tgccaccgat gctcatagat tagtacgata taaaagaaca gatacaaaag
     1201 cttctaaaaa cgattcgttt attgttccca aaaaaccatt aactctttta aaaggtgctt
     1261 tgccaaataa tgacgacgaa ctaagtatta gctataatgc aagccattta tttgtggtac
     1321 atggtaatat acaaatgact tgtagattaa ttgatgcaag atttcctgat tacaaagttg
     1381 taattcctgc agaaaatcct taccatttaa ctgttaataa agatgatttt caaagtgcat
     1441 tacgccgagt tagtgttttt agtaataaaa gtactaatca agtagcttta agcattagca
     1501 gcaacgaatt acaaatggct gctcaagatg tagattttag ttttgaaggt aatgaaagaa
     1561 tgggatgctc atacaatgga gaggatatgc aaatagcatt taatgctaaa tttttgatag
     1621 aaatgttgag tgctgcagat accaatgaag tacgtttaga gttaagtaca ccatcaaagg
     1681 caggcttaat taaaccatct gaacaggaag atggtgaaga tttgttaatg ttagtaatgc
     1741 ctttaatgtt aaataattaa ttttatattt tctaaatatt aagagaggct tccaacctct
     1801 ctttttttat ttagttattt caaatatttc tattttttgt agtgagcggt ttttcaatta
     1861 ttaaataaaa aatactgcaa ataaataaaa ctaatggcaa cacaataata ccttgtaaaa
     1921 ctaaattatt gtagtatcct tttttaaata ttgaaatggt aatatgttgc attataaaag
     1981 ctatcaaagc atagtgtaat aaataaattg aataacacat acaaccaata gccatccata
     2041 tttttttagt aaaaattgca tttaaaattc tgcctttaaa aatgcctgta aataataaaa
     2101 catatccaaa catctcaata atatatttat aaaagctagc atatcctgca aaatagagga
     2161 taattaaaaa gcctataata cctaaaacgt cccataaaaa aaacttagat tctaataaaa
     2221 ttttattact taaataaata tcagcaaata aaatacctaa caaaaaaata ggcaagtagg
     2281 gtacaataga gtaggttaaa tgagcaaccg ttatatagtg gtaaaaaata gaatgtagta
     2341 aagcaaataa aagcacacat cccactaaaa gtactcttct ttttacaaaa ttctttataa
     2401 gaaatatttg catcaaaaat ggcaataaaa gataaaactg aatttcaatc tctaatgtcc
     2461 aggcaacagg gttaatggta cttcttctgt catagattat attatgacta taaaaaacac
     2521 tgctaaaaaa atgaggcatt tcatccttcc aaaactctcc ttgaacaaaa attctaaata
     2581 aaagtaaaat aattaaaaca agcaaataag gtatttctaa acgctttaat ctcttaaaat
     2641 aatatttttt caagctcact ttttcggaat tatataaata atattttata aactgaatag
     2701 ataaaataaa tgcactgata caaaaaaata ttctaacacc ataacctcct aattctaaaa
     2761 aaagagctac accgttggcg tattcaaaat ctacaggtaa gttgctttgc tttataattt
     2821 gtaaactaaa gtggcttaat actacagcta taattgcaat aaatcgtagc ccgtcaattt
     2881 cctttataat tacttgctta tctccttgcc ttataaataa atctacccaa tttttcatta
     2941 atataaaagt aacatt
//
LOCUS       DAOQHP010000287         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22603_length_2950_cov_2.680138, whole genome shotgun sequence.
ACCESSION   DAOQHP010000287 DAOQHP010000000
VERSION     DAOQHP010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22603_length_2950_cov_2.680138"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLY81_08725"
     CDS             complement(<1..577)
                     /locus_tag="PLY81_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30348.1"
                     /translation="MINKKVLYIYGRTEINAAKLSFMPFGINPVFKMAEMGMEIDLYL
                     TQSKTNYYQQIFPSNVKIHFMDNKFIWSSGRAKRFIVKIFMYIFFKTFFKKYDIILGA
                     GQVGISLAGFVNYFKRVKLIYLSDEFPHVYHKSYWKKLEYYFSKKANYFIVPDESRLD
                     ITCKTVGLSPNTGYFLPNIPLSVNIQDNDWFN"
     gene            complement(666..1766)
                     /locus_tag="PLY81_08730"
     CDS             complement(666..1766)
                     /locus_tag="PLY81_08730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF30349.1"
                     /translation="MIPVNTPLLNGNELKYLTQCIETGWISSEGPFIKSFEEKMAAYV
                     GRKHGIAVSNGSAALDVAIKALNLSKGDEVIMPTFTIISPAQSIVNVGAVPVLVDSDA
                     STWNIDVTQIEAKITSKTKAILVVHIYGLPVDMQPVLELCKKYNLLLIEDAAEMHGQT
                     YNGQQCGSFGDISIFSFYPNKHITSGEGGMVMCNDDALAERCRKLRNLAFETNGRRFI
                     HYELGWNYRMTNMQAAIGLAQLEKLDIHIAKKRKIGKLYDEGLKDLKGFQLPLPQTDY
                     AENIYWVYAMVAETEALANKTVQKLNEAKIGTRPFFWCMHEQPVFQKMGLFKNESYPV
                     AEKIARNGFYLPSGLGLSEGEIELVISNLMKL"
     gene            complement(1830..2189)
                     /locus_tag="PLY81_08735"
     CDS             complement(1830..2189)
                     /locus_tag="PLY81_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF30350.1"
                     /translation="MKYVTFEDMNVWKNAMEVATLVFNLTTNLPRSEDYGLTSQLRRS
                     ALSVSANIAEGYGRESNMDKIRFYVISRGSAFETKSHLIYGEKVNYFENEKIHTLNYK
                     IDNVIFELNKIIKTLKP"
     gene            complement(2221..>2950)
                     /locus_tag="PLY81_08740"
     CDS             complement(2221..>2950)
                     /locus_tag="PLY81_08740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30351.1"
                     /translation="SRYYDLLYKDKNYTSEAEYVLNLAKNYNKECNKFLELGCGTGNH
                     AAVLCNKGFSVVGLERSEEMVALAKQKNISNFNPQVADITNFNINEKFDVALSLFHVI
                     SYLTDNEQLINCFNTVHQHLNINGIFIFDVWYSPAVYIQKPETRVKRISDEQIDVTRI
                     AESTVYYNKNVVDVNYTIFIQDKTTKITEVHHETHPMRHFSKPEIELLATQTNFKLLQ
                     AEEFGTANSPSEKTWGVCMVLRKK"
BASE COUNT          940 a          520 c          412 g         1078 t
ORIGIN      
        1 tgttaaacca atcattatct tgtatattaa ctgataatgg aatgtttgga agaaagtaac
       61 cagtattggg tgataaacca acagttttgc aagtaatatc taatctgctt tcatcaggaa
      121 caataaaata gttggctttc ttagaaaaat aatattccaa ttttttccaa taacttttat
      181 gataaacatg cggaaattca tcgcttaagt atattaactt aactctttta aaatagttta
      241 caaatcctgc caaggatata cctacttggc ccgctcccaa aataatgtca tattttttaa
      301 aaaatgtttt aaagaatata tacataaaga tttttacgat aaacctttta gccctaccag
      361 aactccaaat aaatttatta tccataaaat gtattttaac attagatgga aatatttgtt
      421 gataatagtt tgttttgctt tgtgttaaat acaagtcaat ttccataccc atttcggcca
      481 ttttaaaaac aggattaata ccaaaaggca taaaggacaa ttttgctgca tttatttccg
      541 ttctgccata aatatacaat acctttttat tgatcatctt ttgtgtaaac ttttatgctg
      601 ttggcctaaa tttaattgaa ctaataaata atttttgatt caaactccta tttaatgata
      661 aatgattaaa gtttcatcaa attacttata accaattcta tttctccctc actcaatcct
      721 aaaccactgg gaagataaaa accattgcgt gctatttttt ctgcaacagg atagctttca
      781 tttttaaata aacccatttt ttggaaaact ggttgctcat gcatacacca aaaaaatgga
      841 cgtgttccta ttttggcttc atttagtttt tgaacagttt tattggctaa agcttctgtt
      901 tctgcaacca ttgcatatac ccaatatata ttttcggcat aatcggtttg tggcaaagga
      961 agttgaaaac cttttaggtc ttttagacct tcatcgtata gtttgccaat ctttcttttc
     1021 tttgctatat ggatgtctaa tttttctaat tgtgccaaac caatagcagc ttgcatattg
     1081 gtcatacggt aattccaacc caactcatag tgtataaatc ttctgccatt ggtttcaaaa
     1141 gctaaattgc gtaactttct acaacgttca gccaaagcat catcattgca cataaccata
     1201 ccgccttcgc cagaagtaat atgcttattg ggataaaaac taaaaatact aatatctcca
     1261 aacgagccac attgctgccc attatacgtt tggccatgca tttcggcagc atcttcaatt
     1321 aataacaaat tatatttttt acaaagctct aatacaggtt gcatatctac aggcaagcca
     1381 taaatatgta ctactaatat ggcttttgtt ttacttgtta ttttggcctc aatttgtgtt
     1441 acatctatat tccatgtaga agcatcacta tctaccaaaa caggaacagc accaacattt
     1501 actatagatt gagctggtga aataatggta aaggtgggca taattacttc atcgcctttg
     1561 cttaggttta aggcttttat agcaacatct aatgcagcag agccattact tactgcaatt
     1621 ccatgtttac gacctacata agcagccatt ttttcttcaa aagattttat aaatggacct
     1681 tcgcttgaaa tccagccagt ttcaatacat tgggttaaat attttaattc gttaccgtta
     1741 agtaaaggtg tatttacggg tatcataatt tgaggttgag agtgaggttg aggttgagat
     1801 taaggttaag gttgaggtta agattaaggt taaggcttta gtgttttaat tattttgttc
     1861 aactcaaaaa ttacattatc tattttataa tttaaagtat gtattttttc attctcaaaa
     1921 taatttactt tttcaccata aattaaatga ctttttgttt caaaagcact gccccttgat
     1981 attacataaa acctaatttt atccatatta gattctcttc cataaccctc tgcaatatta
     2041 gcactaacag acagtgctga tcgtcttagc tgactggtta atccataatc ttctgaacga
     2101 ggtaaattgg ttgttaaatt aaaaactaaa gtggcaacct ccatggcatt tttccataca
     2161 ttcatatcct caaaagtaac atatttcatt atttacttgc atttatcact ttaccttaac
     2221 ctatttcttt cttagaacca tgcaaactcc ccaagttttt tcgcttggtg aatttgctgt
     2281 tccaaattct tctgcttgta gtagtttaaa atttgtttga gtagctaata attctatttc
     2341 tggtttacta aaatgacgca ttggatgtgt ttcatgatgt acttctgtta ttttagttgt
     2401 tttatcttga ataaaaatgg tatagtttac atctactaca tttttgttat aatagacagt
     2461 tgattctgca atccttgtaa catcaatctg ctcatcacta attcttttca ctctggtttc
     2521 tggtttttgt atatacacag cagggctgta ccacacatca aaaataaaaa taccattgat
     2581 gtttaagtgt tgatgcactg tattaaagca gttaattaat tgttcattat ctgttaaata
     2641 actgattaca tgaaataagg acaaggctac atcaaacttt tcgttgatgt taaaattggt
     2701 aatatctgca acctgaggat taaaattgga aatatttttt tgcttagcca acgctaccat
     2761 ttcctcactt ctttctaagc ctacaacaga aaaaccctta ttgcataaaa cagcagcatg
     2821 attacccgtg ccacagccta attctaaaaa tttgttgcac tccttattgt agtttttagc
     2881 aagatttaaa acatactcag cctcagaagt atagtttttg tctttataca acaaatcgta
     2941 atagcgactg
//
LOCUS       DAOQHP010000288         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22763_length_2938_cov_3.831772, whole genome shotgun sequence.
ACCESSION   DAOQHP010000288 DAOQHP010000000
VERSION     DAOQHP010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22763_length_2938_cov_3.831772"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLY81_08745"
     CDS             <1..793
                     /locus_tag="PLY81_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF30352.1"
                     /translation="KDKTAVDEWSLQNVKLCSQRQQRIIANVMPALKENGYLIYSTCS
                     YSEEENEQILDFIADNFSVESVEININPSWGIVSTKSNKHKAIGYRFFPDKIKGEGFF
                     IAVFKKNEFSKNNKFSLQLLSKLTSVENELIKNFITSTIDKKFFKHNNEIKCINTAFY
                     ADVEKLANLLYLKKVGTIIGEVKGKDFVPHHELAISNISLSNIATLELNKEDALKFLA
                     KKELDINTNKGLTLITFNGLGLGWIKVLHNRINNYYPNEWRILKI"
     gene            816..1757
                     /locus_tag="PLY81_08750"
     CDS             816..1757
                     /locus_tag="PLY81_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNF30353.1"
                     /translation="MKKIFCIVLTFFILNQSFAQNKILVQGTYPNLYLQHKVAHGETM
                     YSISKLYNFSSAQLATQNGMTENDILSTDKILKINLGKNNFTQDGQSGENETLIPLYH
                     IVKPGETLFRICQNFGKIRIDFIRGWNDLVNDIIKPEQKIIIGHLRVDLKKASIVTER
                     IINTDAQETATAIDQTEPPKKDETETTTSTTPKQEEEEPVVEPVTNGNDDEGFFVTSY
                     SVNSDNSVSTTGEAATFKSTSGWTNRKYYVLCNDIPTGTIVRITANNNRSICAKVLGA
                     LPEMKENKNLIIRISNAGAAVLKITEKRFNVKITYNK"
     gene            complement(1761..2648)
                     /locus_tag="PLY81_08755"
     CDS             complement(1761..2648)
                     /locus_tag="PLY81_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30354.1"
                     /translation="MSSSEIDIFKWDNCVQENSNGLIYAYSFYLNTMAENWHGLIIND
                     YEAIFPIPVKNKLGIQYAYMPAFTQQLGLIGNVKINNTKLINAIHSFLKYGSINLNFS
                     NKNFTEKIVEKNNFIIDLKNNYSVIQEKYKKTIAYSLSKAAKQHFIYTKDDDIKKAVA
                     LYKLYNQNNMPHVIDIDYTNLKKLLLQLQKNNNVLVRKVENEKQELMSIALLMKDIKR
                     YYNILNTTTEAGRKTEANYFLYNNILQELCNQNMLFDFEGSDLPGVKKFYEKFGAINQ
                     PYFNWHFNNLPIPFKWLKK"
     gene            complement(2672..>2938)
                     /locus_tag="PLY81_08760"
     CDS             complement(2672..>2938)
                     /locus_tag="PLY81_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30355.1"
                     /translation="PLKFFVDIGTYAESWKENPATGRFLYDAGLQLPLIKGLVNIYIP
                     ILYSKVYADYFKSTLGSKRFSKTISFSIDLQQLQLNKLSRYIPL"
BASE COUNT         1123 a          382 c          414 g         1019 t
ORIGIN      
        1 aaaagataaa acagcagtag atgaatggag tttacaaaat gtaaaacttt gtagccaaag
       61 acaacaaaga attattgcca atgtaatgcc agctctaaaa gaaaatggtt atttaattta
      121 ctccacttgc tcctattcag aagaagaaaa tgaacaaata ttagatttta ttgctgataa
      181 cttttctgta gaaagtgtag agataaatat taatccttct tggggtattg tatctactaa
      241 aagcaataag cataaagcta tagggtacag attttttcct gataaaataa agggagaagg
      301 tttctttatt gcagttttta aaaaaaatga attctctaaa aacaataaat ttagccttca
      361 acttttaagc aaattaacca gcgtcgaaaa tgaactaata aaaaatttca ttacaagtac
      421 tattgataag aaatttttta aacataataa tgaaattaag tgtataaaca ctgcttttta
      481 tgctgatgta gaaaaattag caaatttatt atatttaaaa aaagtaggta caattattgg
      541 tgaagtaaaa ggaaaagatt ttgttcctca tcatgaattg gcaattagta acattagttt
      601 aagtaatatt gcaactcttg agttaaataa agaagacgct ttaaagtttt tagctaagaa
      661 agaattggat attaatacca ataaaggact tactttaatt acttttaatg gcttgggatt
      721 aggatggata aaagtattac ataaccgtat taataattat tatccaaatg aatggagaat
      781 tttaaaaata taaacttact tagtaccaaa ttattatgaa aaaaattttc tgtatagtac
      841 ttacattttt tattttaaac caatcttttg ctcaaaataa aatattagtg caaggtacat
      901 accctaattt atacttacaa cacaaagttg ctcatggaga aacaatgtat agtatcagta
      961 aattatataa tttttccagt gctcaattag ccacgcaaaa cggtatgaca gaaaacgata
     1021 ttctatcaac cgataaaatt ttaaaaatta atttgggcaa aaacaatttt acacaagatg
     1081 gacaaagcgg agaaaatgaa actttaattc ccttatatca tattgtaaaa cctggtgaaa
     1141 cgctttttag aatttgccaa aattttggaa aaataagaat agattttatt cgtgggtgga
     1201 atgatttagt taatgatatt attaagcctg aacaaaaaat tatcattggg catttaagag
     1261 ttgatttaaa aaaagcaagc attgtaacag aaagaattat taataccgat gcacaagaaa
     1321 ccgcaactgc aatagaccaa acagaaccac ctaaaaaaga tgaaacagaa accacaacta
     1381 gcactacacc taagcaagaa gaggaagaac ctgtagtaga gcctgtaaca aatggtaacg
     1441 atgatgaagg tttttttgtt actagctatt ctgtaaatag cgataatagt gtatctacaa
     1501 ctggcgaagc tgcaactttt aaatcaacta gcggctggac caatagaaaa tattacgttc
     1561 tatgtaatga tatacctact ggtactattg taagaataac tgcaaataat aatagaagca
     1621 tttgtgctaa agttttaggt gcattacccg aaatgaaaga aaataaaaat ttaattatta
     1681 gaattagtaa tgctggtgct gcagtattaa aaattacaga gaaaagattt aatgtaaaaa
     1741 ttacttacaa taaataacat ttatttcttc aaccatttaa aaggtatagg taaattatta
     1801 aaatgccaat taaagtatgg ttgattaatt gctccaaatt tttcataaaa ttttttcaca
     1861 ccaggtaaat cacttccttc aaaatcaaac aacatgtttt gattgcataa ttcctgtaaa
     1921 atattgttgt acaaaaaata attggcttca gtttttcgtc ctgcttctgt ggttgtgttt
     1981 aatatattgt aataacgttt aatatctttc attaacaaag caatactcat tagttcttgt
     2041 ttttcatttt ctacttttcg taccaaaaca ttattatttt tttgtagttg caataatagt
     2101 ttttttaagt tggtgtaatc tatatctatt acatgaggca tgttgttttg gttgtacaat
     2161 ttatataatg caactgcctt ttttatgtca tcatcttttg tgtaaataaa atgttgtttt
     2221 gctgcttttg ataaactgta tgcaattgtt tttttgtatt tttcctgaat tacagagtaa
     2281 ttgtttttta aatcaataat aaaattattc ttttctacaa ttttttctgt aaagttttta
     2341 ttggaaaaat tcaaattaat agagccatat tttaaaaatg aatgaatagc atttatcagt
     2401 tttgtattat taatttttac atttcctatt aagcctaatt gttgtgtaaa tgcaggcata
     2461 taagcatatt gaatacccaa tttattcttt acaggaattg ggaaaatcgc ttcataatca
     2521 tttattatca aaccgtgcca attctcagcc attgtgttta aataaaaact ataagcataa
     2581 attaaaccat tagaattttc ttgaacacaa ttatcccatt taaaaatatc tatttcagat
     2641 gatgacaaaa tttttatttg atacataata attataatgg aatatatctg ctaagtttat
     2701 tcaactgaag ctgctgtaag tctatggaga aagaaattgt tttagaaaat ctttttgaac
     2761 ctaatgtaga tttaaaataa tctgcatata ccttactata caaaataggt atataaatat
     2821 ttactaaccc tttaatcaaa ggcagttgta aacctgcatc atataaaaac ctgcctgtcg
     2881 ctgggttttc tttccaagat tcggcataag tgccaatatc tacaaaaaac tttaaagg
//
LOCUS       DAOQHP010000289         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_22897_length_2929_cov_2.881002, whole genome shotgun sequence.
ACCESSION   DAOQHP010000289 DAOQHP010000000
VERSION     DAOQHP010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22897_length_2929_cov_2.881002"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLY81_08765"
     CDS             <1..193
                     /locus_tag="PLY81_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNF30356.1"
                     /translation="VMFSSPEMVVLFGGIAKAGDLLLIPTQKAMEKNLLPIFKNKVKL
                     VLSNLHESDAAILGASALV"
     gene            331..1215
                     /gene="atpG"
                     /locus_tag="PLY81_08770"
     CDS             331..1215
                     /gene="atpG"
                     /locus_tag="PLY81_08770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNF30357.1"
                     /translation="MAGQLKEVRNRIKSVQSTQQITKAMKMVSAAKLRRAQDAITQMR
                     PYAQKLQEMLSNIVSNIDGDTELKLAENRPVEKVLLIVVTSDRGLCGAYNSNIIKLAK
                     STIAQKYSTQYQKGNVAIWNIGKKGWEALTKAGYKTSDTFKDIFQQLSFTNVQAAAQA
                     ATKAFENNEFDAVEIIYSHFKNAATQTFIAEPFLPIPKVESNTNSNKKSDFIFEPNKD
                     ELIAELMPKILNTQLYKAVLDGNASEHGARMTAMDKASDNANELLKTLKISYNRARQA
                     AITTELTEIVGGAAALQG"
     gene            1244..1927
                     /gene="scpB"
                     /locus_tag="PLY81_08775"
     CDS             1244..1927
                     /gene="scpB"
                     /locus_tag="PLY81_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNF30358.1"
                     /translation="MELSAIIPHIEVLIFASEKPLTALEIVELINNSFGFLEERINLD
                     QVESCIEGIVEKYNTEFYPFEVKESGGGFQFLTKKDFHQTVAQLNGDKFLKRLSTAAL
                     ETLAIIAYKQPITKGEIESLRGVNSDYSVQKLLEKELIVIAGRNENLPGHPLLYTTSK
                     SFMDYFGINSTDDLPKIKEILSEQLVEGTIINTDDFTSNTIIDEVETNLPNTENSNNS
                     ISENSNTEQ"
     gene            2037..2873
                     /locus_tag="PLY81_08780"
     CDS             2037..2873
                     /locus_tag="PLY81_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNF30359.1"
                     /translation="MNWAESKKIKIAILDLYEGHANQGMRCIREIINTWAEHNQLEVN
                     FDEFDLRLKEEIPDTSYHIYISTGGPGSPLETIGTNWEQKYFKWLQSIEEWNNNATNI
                     PKHVFFICHSFQLICRHYKIGNVCKRKSTSFGVFPVHKLEEGNEEMVFENLKEPFYVV
                     DSRDYQVIEPNYSILREMGAKILAIEKNRPHVPFERAIMAVRFNDYFIGTQFHPEADA
                     IGMKLHLLTEEKKATVILNHGEEKWRSMVEQLGDPEKILWTYNHILPNFLNHAVGVLT
                     EA"
BASE COUNT         1135 a          425 c          479 g          890 t
ORIGIN      
        1 tgtaatgttt tcctctccag aaatggttgt tctttttgga ggcatagcca aagcaggaga
       61 tcttttactt attcctacac aaaaagcaat ggaaaagaac ctcttaccca tttttaaaaa
      121 taaagtaaaa ttagttttaa gcaatttaca cgagtccgat gctgctatat taggtgcaag
      181 tgcattggta taacgtaact aaaaacagtt agtaaaaccg cctaaaaacc aatagttttt
      241 cacattaaaa actgaatatg gtattttagc cttattctgc tacttttgca gccaatttta
      301 actattaaag ttttataaac ttaattcaaa atggcaggtc aattaaaaga agtaagaaat
      361 agaataaaaa gcgtacagag cacacagcaa attaccaaag ctatgaaaat ggttagtgca
      421 gcaaagttac gcagggcaca agatgctata acacaaatgc gtccttatgc acaaaaatta
      481 caagaaatgc ttagcaatat tgtaagtaat attgatggcg atacagaatt aaaacttgcc
      541 gaaaatagac cagtagaaaa agtattgctt attgttgtaa ctagtgatag aggtttatgt
      601 ggtgcctaca acagtaatat aataaaacta gcaaaaagta ccattgctca aaagtattct
      661 acccaatatc aaaaaggaaa tgtggctatt tggaatattg gtaaaaaggg ttgggaggct
      721 ttaaccaaag caggctataa aaccagcgat acttttaaag atatctttca acaactatct
      781 tttactaatg tacaagctgc tgcacaagca gcaactaaag cttttgaaaa taacgagttt
      841 gatgcagtag aaattattta tagccatttt aaaaatgcag caacgcaaac ttttatagca
      901 gaaccttttt tacctatacc taaagtagaa agcaatacta atagtaataa aaaatctgac
      961 tttatttttg agccaaataa agatgagtta attgcagaat tgatgcctaa aattttaaat
     1021 acacagttat acaaagcagt tttagatggc aatgctagcg aacatggtgc tagaatgaca
     1081 gcaatggata aagcaagtga taatgcaaat gaattattaa aaacattaaa aatttcgtac
     1141 aaccgtgcaa ggcaagcagc aattacaaca gaacttacag aaatagtagg cggagcagca
     1201 gctttacaag gataatttaa aaaaataaac ctatcttttg caaatggagt tatctgctat
     1261 tataccacat attgaagtat taatttttgc tagtgaaaaa cctttaacag cactagaaat
     1321 tgtagaacta ataaataact cttttggttt tttagaagaa agaataaatt tagaccaagt
     1381 tgaaagttgt atagaaggaa ttgttgaaaa atacaatacc gaattttatc catttgaagt
     1441 gaaagaaagt ggtggtggtt ttcagttctt aactaaaaaa gattttcatc aaactgttgc
     1501 acaacttaat ggagataaat ttctaaaaag gctatctacc gctgctttag aaactttagc
     1561 catcattgca tacaagcaac caattaccaa aggcgaaatt gaaagtttaa gaggcgtaaa
     1621 tagtgattat agtgttcaaa aattactaga aaaagaactt attgtaattg caggcagaaa
     1681 cgaaaacctt ccagggcatc cacttttata tacaacttca aaatcattta tggattattt
     1741 tggtattaat tctaccgatg atttaccaaa aataaaagaa atactttctg aacaacttgt
     1801 agaaggtacc atcataaaca cagatgattt taccagcaat acaattattg atgaagttga
     1861 aactaacctt ccaaatacgg agaacagtaa caattcaatt tcagaaaatt ccaacaccga
     1921 acaataaatc tcttttccta aaaaattaaa aactattaat tttgagaagc tttattcaat
     1981 aatgtgtata aagcattgga ttagttgtat taatttatta ttatgtttgt tatagaatga
     2041 attgggcaga atcaaagaaa ataaaaattg caatattaga tttatatgaa gggcatgcca
     2101 atcaaggtat gcgttgtatt agagaaatta taaacacttg ggcagaacat aatcagctag
     2161 aagtaaactt tgatgagttt gatttaaggc ttaaagaaga aattccagat acatcttatc
     2221 atatttatat ttcaactggt ggtccaggtt cacctcttga aactattggc actaattggg
     2281 agcaaaaata ctttaaatgg ttacaatcaa tagaagaatg gaataataat gcaaccaata
     2341 ttccaaagca tgttttcttt atttgtcata gctttcaatt aatttgcaga cactataaaa
     2401 taggcaatgt ttgtaaaaga aaatctacct catttggcgt ttttcctgtt cataaacttg
     2461 aagaaggaaa tgaagaaatg gtttttgaaa atttaaaaga acctttttat gttgtagata
     2521 gcagagacta tcaagtaatt gagcctaact attctatctt aagagaaatg ggtgctaaaa
     2581 ttttggcaat cgaaaaaaat aggcctcatg tgccctttga aagagccatt atggctgtaa
     2641 gatttaatga ttattttata ggaactcaat ttcatccaga ggcagatgct attggaatga
     2701 aattacattt acttaccgaa gaaaagaaag caactgtaat tttaaatcat ggagaagaaa
     2761 aatggagaag catggtagaa caattaggcg atccagaaaa aattctttgg acatataatc
     2821 acattttacc aaatttctta aaccatgctg taggagtatt aacagaagca taatttttta
     2881 tcagatttct taactctcct gctgtttttt cacaatctta atttatctt
//
LOCUS       DAOQHP010000290         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23076_length_2916_cov_3.299546, whole genome shotgun sequence.
ACCESSION   DAOQHP010000290 DAOQHP010000000
VERSION     DAOQHP010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23076_length_2916_cov_3.299546"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="PLY81_08785"
     CDS             complement(<1..2115)
                     /locus_tag="PLY81_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF30360.1"
                     /translation="MQDYLYGLNEPQKEAVLHINGPLMIIAGAGSGKTKVLTTRIANL
                     MANGVDAFNILALTFTNKAAAEMKERIERILGNNEARNLYIGTFHSVFARILRAEAGK
                     IGYTNNFTIYDTDESRSVLKTIITEMELDVKHYKPNFIHNRISAAKNALISADEYEQN
                     LYLTKEDEKANRPAIAKIYKAYVARCFKNGAMDFDDLLFKMYELLNNNPETLLKYQHK
                     FKYVLIDEYQDTNPVQYQITKLLAAVYENICVVGDDAQSIYSFRGATIENILQFQKDY
                     DDVKVIKLEQNYRSSQSILNVANTIIGNNKGQLPKELWTQNDLGEKIKVVRTNTDNDE
                     GKLVADAIKENKLRYHFNNNDFAILYRTNAQSRAFEESLRKQGIAYKIYGGMSFYQRK
                     EIKDMLAYLRLIVNINDDEALKRIINYPIRGIGKTTIEKLAIIAYEQNINMYNVMQRA
                     SEFGFKAGTLELLQNFVTMIKYFQSILTDKNAYDVAVMVGKHTNLVKELFNDKSTEGL
                     ARYDNLQELLNSIKEWTESPDNEDGEIGSKSLGAYLQQISLLTDADEDKENSDVVKLM
                     TIHAAKGLEFACVFVGGLEETLFPNAMSLNTREELEEERRLFYVAITRAKQKLWISYS
                     NSRYKFGNLVINEPSRFLDEMPKNFLDESYANIARNEFNNSNAYARMSGNYGYNNKSQ
                     QFTKKEAERKPTYLPINPPTPKV"
     gene            2299..>2916
                     /gene="recA"
                     /locus_tag="PLY81_08790"
     CDS             2299..>2916
                     /gene="recA"
                     /locus_tag="PLY81_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNF30361.1"
                     /translation="MSNVEKLKALKLTIDKIDKDFGKGSVMMMSDRADRQIEVISTGS
                     IGLDAALGVGGVPKGRIVEIYGPESSGKTTVATHIIAEAQKKGGICAIIDAEHAFDSS
                     YAQKLGVDVESLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALVPKGELEGEMG
                     DSKMGLQARLMSQALRKLTATINKTNTICIFINQLREKIGVMFGNP"
BASE COUNT          916 a          458 c          456 g         1086 t
ORIGIN      
        1 aacttttggt gttggtggat ttataggtaa atatgttggt tttctttcgg cctctttttt
       61 ggtaaattgt tgagatttgt tattgtaacc ataattccca ctcattcttg cataagcatt
      121 actattgtta aattcatttc ttgcaatatt agcataagac tcgtctaaaa agtttttagg
      181 catctcatcc aaaaatcggc ttggttcatt tattactaag ttgccaaatt tgtaccttga
      241 attggaataa gaaatccaca gtttttgctt tgctcttgta atggcaacat aaaataacct
      301 tctttcttct tctaattctt ctcttgtatt taaagacatt gcattaggaa acaaagtttc
      361 ttctaagccg cctacaaata cacaagcaaa ctctaaacct tttgctgcat gtatagtcat
      421 tagttttact acatctgaat tttctttgtc ttcatcagca tcggttaata aagaaatttg
      481 ctgtaaataa gcacccaaac ttttacttcc aatttctcca tcttcattgt ctgggctttc
      541 tgtccattct ttaatagagt ttaataactc ttgtaagtta tcgtatcttg ctaagccttc
      601 tgtagattta tcattaaata attctttcac taaattagta tgcttgccaa ccattacagc
      661 aacatcataa gcatttttgt cggttaaaat gctttgaaaa tatttaatca ttgttacaaa
      721 attttgtaac agctctaaag tgcctgcttt aaagccaaac tcgctagcac gttgcataac
      781 attatacatg tttatgtttt gctcataagc aataatggct aatttttcaa tggttgtttt
      841 tcctatgcct ctaatagggt aattaataat tcttttaagt gcttcatcat cgttaatatt
      901 tacaataagc cttaaatagg caagcatatc ttttatttct ttacgctgat aaaaactcat
      961 gccaccataa attttatagg caataccttg ctttcttaaa ctttcttcaa aagctcggct
     1021 ttgggcattg gttctgtata aaatggcaaa gtcgttattg ttaaagtggt atcttaattt
     1081 attttcttta atagcatctg ctactaattt accttcatca ttatctgtat tggttctaac
     1141 tacttttatt ttttctccta aatcgttttg tgtccaaagt tcttttggca attggccttt
     1201 gttattacca attattgtat tggctacatt taaaatactt tgagagctac gataattttg
     1261 ttcaagtttt atcactttta catcatcata atctttttga aattgtaaaa tgttttctat
     1321 ggtagcacct ctaaaactat aaatactttg tgcatcatct cctaccacac aaatattttc
     1381 atatacagca gctaataatt tggttatttg atattgtaca gggttagtat cttggtactc
     1441 atcaattaaa acatatttaa acttgtgctg gtactttaat aatgtttcag gattgttgtt
     1501 aagtagttca tacattttaa aaagcaaatc atcaaaatcc atagcaccgt ttttaaaaca
     1561 acgggctaca taagctttat aaattttagc tatggcaggt ctattggctt tttcatcttc
     1621 ttttgtaagg tataaatttt gttcatattc atcggcacta attaatgcat ttttagcagc
     1681 cgatattcta ttatgtataa agttgggttt gtaatgtttt acatctaatt ccatttcggt
     1741 aatgatggtt tttaaaacac ttctactttc atcggtatcg taaatagtaa aattattcgt
     1801 ataaccaatt tttcctgctt cggctcttaa aattcttgca aaaacactat gaaaagtacc
     1861 aatgtataaa tttctggctt cgttgtttcc taaaattctt tcaattcttt ctttcatttc
     1921 ggcagcagct ttgttggtaa aagttaatgc caaaatatta aaagcatcta caccgtttgc
     1981 cattaaatta gcaatacgtg tagtaagaac ttttgtttta ccactacctg caccagcaat
     2041 aatcattaaa ggcccattaa tgtgtaatac tgcttctttt tgtggctcgt ttaagccata
     2101 taaataatcc tgcatggctg caatttatag gaagtaaaat tggaaattag ctttagggga
     2161 taagtttagt aatttaaaac tgcataaaat tggtttgttc ataattttag caaaaaatac
     2221 taaaaaattt agtgttaata ataaaaacta cttactttta cagtgtaaac taattttaac
     2281 cgataaaaat ttatagtaat gagtaatgta gaaaaattaa aagcactgaa attaactatt
     2341 gataaaatag ataaagattt tggtaaagga agtgttatga tgatgagtga cagggcagac
     2401 aggcaaatag aagtgatcag tacaggctca ataggtttag atgctgcttt gggcgttggt
     2461 ggtgtaccta aaggaagaat tgtagaaatt tatggcccag aaagtagtgg taaaacaacc
     2521 gttgcaacac atattatagc agaagctcaa aagaaaggtg gtatttgtgc tattattgat
     2581 gctgaacatg cttttgatag ttcttatgct caaaaattag gggtagatgt tgaaagttta
     2641 ttaatctctc agccagatta cggagaacag gctttagaaa ttgcagacag attaatactt
     2701 tctggtgcat tagatgtggt agtgatagac tctgtagctg cattagtacc caaaggagaa
     2761 ttagaaggtg aaatgggtga tagtaaaatg ggtttacagg ctcgtttaat gagccaggct
     2821 ttacgtaaat taacagccac cataaacaaa acaaatacta tttgtatttt cattaatcag
     2881 cttcgtgaaa aaattggcgt aatgtttggt aatcca
//
LOCUS       DAOQHP010000291         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23255_length_2903_cov_3.811096, whole genome shotgun sequence.
ACCESSION   DAOQHP010000291 DAOQHP010000000
VERSION     DAOQHP010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23255_length_2903_cov_3.811096"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLY81_08795"
     CDS             <1..938
                     /locus_tag="PLY81_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30362.1"
                     /translation="SNSNLHSEELNIQENNQSNLIKGTTEKVFLLTTLPLAKIANQKL
                     SYKLYQNTEPLECPGEYSSYKKNWYVEGFIAPEYVMKKVYSNNPANDAYLSKKDSVEK
                     MVAGISIGAKFTRGLSKNLYIKGGLQYNQMIEKTSLMMEKERKEITVITINTVTDING
                     NTVTVADTSKQLQITYARSVNYNSIKSIELPISLGYEFYRKNFRASVNAGFIVNLSSW
                     YSGKIFDTSYQMVNIKERPGIYKQNVGLSLYGSISLIKPLSESAEIFAEPYFRYSLTN
                     PKYNAYGFKQKFNAVGLSMGLRFKLNKNYSSRTFN"
     gene            1023..>2903
                     /locus_tag="PLY81_08800"
     CDS             1023..>2903
                     /locus_tag="PLY81_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF30363.1"
                     /translation="MQLSIIIVNFNVKYFLQQCLYAVQKAINTIDAEVFVVDNNSTDG
                     SVAYLQPFFPWVKFIQNKENIGFGKANNIAFAQAKGEFILFLNPDTIVPEDCFTKCIA
                     FINKQTNCAALGIKMLDGQGHFLPESKRSFPSPITSFYKLIGASYLFPTSKHFNKYAL
                     GYLDKNKNHEVDVLAGAFIMTRKELLDKIGVFDEKFFMYGEDVDLSYRLQQTGLKNYY
                     FADSCIIHFKGESTKRGSLNYVKMFYEAMSIFVSKHYKGSYATIFSSTIKIAIWIRAV
                     LSLLKRLFTKISLAIIDSLVIYFCIWLVKTAWVTYIRHGNEFLQNIVNTITPIYVLVF
                     LTAAAFAGMYDNLYKPVKAIAASLVAIVAGLAVYSLLPENVRFSRGVILFGGITSGFM
                     LIFYRWVLTKMGLIKETDEENKMQQTVIVGTIEEYTNAFNLLKTVNKQERVLGRIDVL
                     NEPNGNSIGSFNQLQKIIETLKIKEIIFCGGTLSYQQIINQVQQLSGKINFRFYENES
                     SCIIGSDSKTATGEILNTEVVYSITNPYQARMKRVVDIFTALFILVTFPFQLLFIKKT
                     ANAILNALKVLIGKNTWVGYANLTPTLPVLKPAIITHYGIATKKKQFANTNVLEKLDF
                     IYA"
BASE COUNT         1086 a          358 c          483 g          976 t
ORIGIN      
        1 tatccaatag taatcttcat tcagaagaat taaatattca agaaaataat caaagtaatt
       61 taataaaagg cactactgaa aaagtattct tacttactac acttccatta gccaaaattg
      121 caaaccaaaa actatcttat aaactatatc aaaatacaga accattagaa tgcccaggag
      181 aatatagtag ttataaaaag aattggtatg ttgaagggtt tattgcacca gagtatgtga
      241 tgaaaaaagt atatagtaat aacccggcaa atgatgctta tttaagcaag aaagatagtg
      301 tagaaaaaat ggttgcaggt attagcatag gtgcaaaatt tacaagagga ttatctaaaa
      361 atttatacat taaaggcggt ttacaataca atcaaatgat tgaaaaaaca agcctgatga
      421 tggaaaaaga aagaaaggag attactgtaa taaccattaa tacagttact gatattaacg
      481 gaaatactgt aacagttgca gatacttcta aacaattaca aataacttat gcccgtagtg
      541 ttaattacaa ttcaataaaa agtatagagt tgccaatttc tttagggtac gaattttata
      601 gaaaaaattt cagagcatct gttaatgctg gctttattgt aaacctatct agttggtata
      661 gtggtaaaat atttgataca agttatcaaa tggtaaatat aaaagaacgc cctggaattt
      721 ataaacaaaa tgtaggctta agtttgtatg gtagcatcag tttaataaag ccattatccg
      781 aatctgctga aatatttgca gaaccctatt ttcgttacag tttaacaaac ccaaagtata
      841 atgcctatgg gtttaaacaa aaatttaatg ctgttggatt atcaatgggg ttgagattta
      901 aattaaataa aaattactca agcagaactt ttaactaaat ttattttctc aaaaatttta
      961 ttaagttttg gcaacttcgt aaatacaaag tatgttgcct gcaaataagt ttgtataaat
     1021 aggtgcagct atcaataata atagtaaatt ttaatgtaaa atattttctt caacagtgtt
     1081 tgtacgctgt acaaaaagct attaatacta ttgatgcaga agtttttgtg gtggataata
     1141 attcaacaga tggtagtgtt gcctatttac aacccttttt cccatgggtg aaatttatac
     1201 aaaataaaga aaatatagga tttggaaagg caaataatat tgcttttgca caagctaaag
     1261 gagaatttat tttatttcta aatccagata ctatagtacc agaagattgt tttacaaaat
     1321 gcattgcctt tataaataag caaacaaatt gtgcagccct aggaattaaa atgttagatg
     1381 ggcagggcca ttttttacca gaaagtaaaa gatcttttcc gtctccaatt acatcattct
     1441 ataagctcat aggggcttcg tatttatttc caacttctaa gcattttaat aaatacgctt
     1501 taggatattt agataaaaat aaaaatcatg aagtggatgt tttagctggt gcttttataa
     1561 tgacaagaaa ggagttatta gataagattg gtgtttttga tgagaagttt tttatgtatg
     1621 gcgaagatgt agatttaagt tataggttac aacaaactgg acttaaaaat tattattttg
     1681 cagatagttg cattattcat tttaaaggag aaagtaccaa acgtggcagc ctaaattatg
     1741 taaaaatgtt ttacgaagca atgagcattt ttgtaagcaa acattataaa ggcagctatg
     1801 ctaccatttt ttcttcaact attaaaattg ccatttggat aagagcagtt ttatctttat
     1861 taaaaaggtt gtttaccaaa attagtttag ctataattga tagtttggta atttattttt
     1921 gtatatggtt agtaaaaaca gcatgggtta cttatataag gcatggtaac gagtttttac
     1981 aaaatattgt aaacacaatt acgccaattt atgttttagt ttttttaact gctgcagcat
     2041 ttgcaggtat gtacgataat ttgtataagc ctgttaaagc cattgcagct tcgttagtag
     2101 caattgttgc aggattagcg gtatattcac ttttgccaga aaatgttaga ttttctagag
     2161 gagtaatatt atttggtggc attacctctg gctttatgct tattttttac agatgggtat
     2221 taactaaaat gggattgatt aaagaaacag atgaagaaaa taaaatgcaa caaacggtta
     2281 ttgtaggcac aattgaagag tacacgaatg catttaattt acttaaaact gtaaataagc
     2341 aagaaagagt tttaggaaga atagatgttt tgaacgaacc aaatggcaat tcaattggta
     2401 gctttaatca gctacaaaaa attatagaaa cattaaaaat taaagaaatt attttttgtg
     2461 gcggaacatt atcttaccag caaataatta atcaagtgca gcaattatct ggtaaaatta
     2521 attttagatt ttatgaaaat gaaagtagtt gtattatagg cagtgattct aaaacggcaa
     2581 ctggtgaaat tttaaataca gaagttgtat attctattac taatccttat caggcaagaa
     2641 tgaaaagagt ggtagatatt tttactgccc tttttatttt agttactttt ccttttcaat
     2701 tactttttat caaaaaaact gctaatgcaa ttttaaatgc actaaaagta ttaataggta
     2761 aaaatacttg ggttgggtat gcaaatttaa cacctacttt gccagtttta aaaccagcaa
     2821 ttattacgca ttacggtatt gcaacaaaga aaaaacagtt tgctaatacc aatgttttgg
     2881 agaaattaga ttttatttat gct
//
LOCUS       DAOQHP010000292         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23283_length_2901_cov_3.385453, whole genome shotgun sequence.
ACCESSION   DAOQHP010000292 DAOQHP010000000
VERSION     DAOQHP010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23283_length_2901_cov_3.385453"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLY81_08805"
     CDS             complement(<1..723)
                     /locus_tag="PLY81_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF30364.1"
                     /translation="MLQLFKYTVIGSLIITLAACGAKNDSLSSKKASLDKLKKEQTAI
                     NEKIKVLESEIAKLDTAFVKNDIAKLVAISSITTQDFTHYIDLQGHIDADNISYISPR
                     LGPGQVKAIYVKKGDFVKKGQLLLKLDDALIKQQINAAKQSLETIKTQLAFAKDIYNR
                     QNNLWKQGIGTEVQLISARTNVETLEKQLSAAQENVKIIQEQASATNVYSDVDGIADE
                     VNIRVGETFSGISATGAQIKIVN"
     gene            complement(754..2088)
                     /locus_tag="PLY81_08810"
     CDS             complement(754..2088)
                     /locus_tag="PLY81_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF30365.1"
                     /translation="MQISKTKQIVLIAILLINYSTIKAQTKHSFTITQTVEYASKNNA
                     QVKNALLGILAQEQVNREVTANAYPTIGADITVNNNIVVSTQVIPGDMFGQPGQLVPV
                     KFGIKYNSNAMIQLRQLLFDGQVFVGLQARKASMEFAKKNAEVVVENIKVNIYKIYYQ
                     LVVSKTQIELLNTNIERIEKLKHDANQLYINGFAEKLDVDKIDVTLTNLKTERVKALN
                     NIEIGYLGLKTLIGMPLNDTLELTDKITDNDIKQNALTDSINFNDRKDFQYLQTVKKL
                     NEYNIKRYKLSYLPTVAISGVYGKQAYRQKFTFLGKGDWFDVSNIGLNISIPIFSGFA
                     RDSKIKQTKIELQQTENNIGNLKLAINNEVESAKLRFNSAIESMDFQKKNMQLAETVY
                     NQTKKKFEAGTGSNTEINAAHTDLKTAQTNYITSLYDAIIAKVDFLKATGKL"
     gene            complement(2094..2699)
                     /locus_tag="PLY81_08815"
     CDS             complement(2094..2699)
                     /locus_tag="PLY81_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF30366.1"
                     /translation="MQQRIVEEAHKLFLTYGIRSISMDEIALKLGISKKTIYQYYKDK
                     DELVDAVFNIEMHQHKVDCECNIVKCKDAIHEVFLALDSVKETFKAMHPLIFFDLMKY
                     HPGVYKKFNTHKTNFFYEVIKSNIERGVQEGLYRPDINIDIISKYRVVCIFQILENEQ
                     FQEVKVPIVEIMQETTEHFLYGLATPKGARLVKKYTQKRTK"
BASE COUNT          832 a          434 c          394 g         1241 t
ORIGIN      
        1 atttacaatt tttatttgtg caccagttgc actaatgcca ctaaaagttt ctcccactct
       61 aatgtttact tcatcagcaa tgccatccac atcgctataa acatttgttg cactggcttg
      121 ttcttgaata atttttacat tttcttgggc tgcacttaat tgtttttcta atgtttctac
      181 attagttctt gcactaatta attgtacttc tgtacctata ccttgtttcc ataaattatt
      241 ttgcctgtta taaatatctt ttgcaaaagc aagttgggtt tttatagttt ctaaactttg
      301 tttggcagca ttaatttgtt gtttaattag tgcatcatct aactttaaaa gcaattgccc
      361 cttttttaca aaatctcctt ttttaacata aattgctttt acttgaccag gtcctaaacg
      421 tggtgaaata taagaaatat tatcagcatc aatatgtcct tgaaggtcta tataatgggt
      481 aaaatcttga gtagtaatag aagaaatagc aacaagttta gcaatgtcat tttttacaaa
      541 agctgtatct aatttggcaa tttctgattc taatacttta attttttcat taattgctgt
      601 ttgttctttt ttaagtttat ctaagctagc ttttttactg cttaacgaat cgttttttgc
      661 tccacaagcc gctagggtta ttattaaaga acctattact gtgtatttga ataattgtaa
      721 catatatggt ttggtttata atattttgaa atgttatagt ttgccggttg cttttaagaa
      781 atcaactttt gcaattattg catcgtataa agaagtgata taattagttt gtgcagtttt
      841 taaatcggta tgtgcagcat taatttctgt attgctacca gtacctgctt caaatttctt
      901 tttggtttgg ttgtaaactg tttcagctaa ttgcatattt tttttctgaa aatccatact
      961 ttcaatagcc gaattaaaac gtagtttggc actttctact tcgttattta ttgccaactt
     1021 taggttgcca atattatttt cagtttgttg taattcaatt ttagtttgtt taattttaga
     1081 atctcttgca aagccactaa aaataggaat cgaaatattt aatccaatat tagatacatc
     1141 aaaccaatcg cctttaccta aaaaagtaaa cttttgacga taagcttgtt tgccatatac
     1201 accactaatt gcaactgtag gtaagtagct taatttgtat cttttaatat tgtattcatt
     1261 caattttttt actgtttgta aatattgaaa atcttttcta tcgttaaaat tgatactatc
     1321 agttagagca ttttgcttaa tatcattatc tgttattttg tctgttagtt ctaatgtatc
     1381 gtttaaaggc ataccaatta atgtttttaa acctaaatag ccaatttcaa tattattgag
     1441 ggcttttact ctttctgttt ttaaattagt taatgttaca tcaattttat ctacatctaa
     1501 tttttctgca aagccattaa tataaagttg gttggcatcg tgttttaact tttctattct
     1561 ttcaatatta gtatttaata attctatttg tgttttacta actactagtt gatagtatat
     1621 tttgtagata tttactttta tattttccac tactacttca gcattttttt tagcaaattc
     1681 catagatgct tttctagctt gtaacccaac aaatacttga ccatcaaata atagttgtct
     1741 taactgaatc attgcattgg aattgtattt aataccaaat tttacaggta caagttgtcc
     1801 tggttgtcca aacatatctc ctggtataac ttgggtagat accacaatgt tattgtttac
     1861 tgtaatgtct gcacctattg ttgggtatgc atttgcagtt acttctctat ttacctgttc
     1921 ttgtgctaaa atgcctaata gtgcattttt tacttgtgca ttatttttac ttgcgtactc
     1981 taccgtttgg gtaattgtaa atgaatgttt ggtttgtgct tttattgtag aatagttaat
     2041 gagtaggatt gctattagta caatttgttt tgttttactt atctgcatat attctatttt
     2101 gttctttttt gtgtatattt ttttactagt cttgctcctt ttggtgttgc taagccatat
     2161 aaaaaatgtt ctgttgtttc ttgcataatt tctactattg gcacttttac ttcttgaaat
     2221 tgttcatttt ctagaatttg aaatatgcat actaccctgt atttactgat gatgtcaata
     2281 ttaatatctg gcctgtacaa accttcttgt actcctcttt caatattgct tttaatcact
     2341 tcgtaaaaaa aattggtttt gtgggtattg aattttttat acacacccgg atggtatttc
     2401 attaaatcaa aaaatattaa agggtgcatt gctttaaaag tttcttttac cgagtctaat
     2461 gccaaaaaca cttcgtgaat ggcatcttta cacttaacta tattacattc acaatcaact
     2521 ttgtgctgat gcatttctat attaaaaaca gcatctacca attcatcttt gtctttatag
     2581 tattgataaa ttgttttttt actgatacct aattttaaag ctatttcatc catacttata
     2641 cttctaatgc cataggttag aaataattta tgggcttctt ctacaattct ctgctgcata
     2701 actaaattat tttgtgtttt tcagggtgcg aaactatgga aactttttat tgcacaaaag
     2761 ttttttacgt tttttttgaa aattttttgt gaatgggtat tagtttttat gaatggcaaa
     2821 gtaaacaaca tgcatatttg tattactttt aagctcagat tatatatttt atgaaactta
     2881 cgttaaaaaa atattttaaa a
//
LOCUS       DAOQHP010000293         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23527_length_2882_cov_2.484259, whole genome shotgun sequence.
ACCESSION   DAOQHP010000293 DAOQHP010000000
VERSION     DAOQHP010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23527_length_2882_cov_2.484259"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2882)
                     /gene="sprA"
                     /locus_tag="PLY81_08820"
     CDS             complement(<1..>2882)
                     /gene="sprA"
                     /locus_tag="PLY81_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF30367.1"
                     /translation="SSAAKVWPDSNRLDFSLQELINLKLRRNASGSPLSTIYREQVGF
                     KTYSVYGNPNLGEVRGILVAVENAKDNNPFDLYSEVWINELRLSQLDERGAYAALGRI
                     DVQLADLGSLSFSANTYSKGFGTIEQRVNERARDNMFQFDAAASIDAGKLVPKSAKIS
                     IPIYASINKTIKRPEYDPYDKDVKLKDKINSAANSRQRDSIKNTALDQTTIKTINFTN
                     IRVQGNGKNKLWSLSNFDVSYSYTKTEQSSPLVLKNDVTKQRGGFGYTFNNSSKYIEP
                     FKKLLVKRKSNWYNLIRDFNFNYMPSLLSFRADINRQFGEYIPRIVSTDGTGKIERVD
                     TTYDKYFTFDRFYNLRWDLTRSLNFDFSATNNARIDEPEGAIDTKEKRDTIRDKFWSG
                     GRNTLYNQRAVASYTLPLAKLPLTDWVTSRYSYTTTYNWIGASLLALPLGNIIENSQE
                     NTFNAEFDFTRLYSKFKILRALDNAPSSTGKNNKVVTPKRTTPTVTLKTKAEVLKGLK
                     GKKRIAALKKWRQQRRDARIAARLIKQNQPVEMNDVLRTTGKMVTMVKRASINYNETF
                     RSRIPGFMGRANHLGNNLKTMQPGLDFVFGMQPDSNWLNTKAAQGILTRDTLFNTLFR
                     QNFEQRLSVTVQAEPLKELIIDLNLDKSFSKEFTELFKDVSNTNKYQHLNPYAAGGFS
                     VSYISFKTLFQKTNPNEISETFKKFQDYRLIMSKRVAETNPYWNGQYDADGFAKGYGR
                     YAQDVLIPSFLAAYTGKDPKNISLLNQNNKNIRSNPFGGILPKPNWRVTYTGLTRIPA
                     LAKKFSTISFSHAYNGTVSMNSYNSALLFADPFRYGAPQFIDTVSGNYIPFFLVPNIT
                     IQEQFAPLIGIDVTTLSQLNVKFEFKKSRQLSLSLIDYQLSETNSTEWVVGAGFRKRG
                     LSFIKIKGKALENDINFRLDLSMRDDATTNSRLDQANAYGTGGQKV"
BASE COUNT          858 a          492 c          429 g         1103 t
ORIGIN      
        1 attacttttt gcccaccagt accataagca ttagcttggt ctaaacggct atttgttgtg
       61 gcatcatctc tcatagaaag gtctaatcta aaattaatat cattctctaa tgccttacct
      121 tttattttaa taaaactaag accacgtttg cggaaacctg caccaacaac ccattcagta
      181 ctgtttgttt cacttaattg ataatctatt aaacttaaac ttaactgacg gctttttttg
      241 aattcgaatt ttacatttaa ctgactaagg gttgtaacat ctataccaat taatggtgca
      301 aactgttctt gtatggttat gtttggtact aagaaaaatg gaatataatt gccactaaca
      361 gtatctataa attgtggtgc accatatcta aaaggatctg caaataataa tgcactattg
      421 taactattca tacttacagt accattgtaa gcatgagaaa aactaatggt gctgaatttt
      481 tttgctaaag caggaattct tgttaaaccg gtataagtta ctctccaatt aggttttggc
      541 aaaatgccac caaaaggatt agaacgtata tttttattat tttgatttaa taaagaaata
      601 tttttagggt ctttacctgt atatgctgct aagaaagatg gaattaaaac gtcttgtgca
      661 tatctaccat acccttttgc aaaaccatct gcatcatatt gcccattcca ataaggatta
      721 gtttctgcaa ctcgtttact cattattaaa cgatagtctt gaaacttctt aaatgtttct
      781 gatatttcgt ttggattggt tttttgaaat aatgttttaa aagaaatata ggatacacta
      841 aaaccaccag cagcataagg atttaagtgt tggtatttat ttgtgttaga tacatcttta
      901 aaaagttcgg taaattcttt actaaatgat ttgtctaaat taaggtctat aattaattct
      961 tttaatggtt ctgcctgtac tgttacactt aaacgttgtt cgaaattttg acggaataaa
     1021 gtattaaata aagtatctct tgttaaaata ccttgagctg ctttagtgtt taaccaattg
     1081 ctatctggct gcataccaaa aacaaaatct aatcctggct gcattgtttt taaattattt
     1141 cctaaatgat ttgccctacc cataaagcct ggaattctac ttctaaatgt ttcgttataa
     1201 ttaatagatg ctcgtttaac cattgttacc attttaccag tagttcttaa aacatcattc
     1261 atttctactg gttggttttg tttaattaaa cgtgctgcta ttcttgcatc tcgtctttgc
     1321 tgtctccatt tttttaatgc tgctattctt tttttgcctt tcaagccttt tagtacttct
     1381 gcctttgttt taagagttac cgtaggagtt gttctttttg gggtaactac tttattattt
     1441 tttcctgttg aagatggtgc attatctaat gctcttaata ttttaaactt actgtataaa
     1501 cgagtaaaat caaattcggc attaaatgta ttttcctgac tgttttcaat tatatttcca
     1561 agaggtaatg ccaataagct tgcaccaatc cagttataag ttgtggtata actgtaacga
     1621 gaggttaccc aatctgttaa tggtagttta gcaagaggta aggtatagct tgcaacagct
     1681 ctttggttgt ataaagtatt tcgtccgcca ctccaaaatt tatctcttat agtatctctt
     1741 ttttctttag tatcaatggc tccttctggt tcatcaattc ttgcattatt ggttgcagaa
     1801 aaatcaaagt ttaatgaacg ggttaaatcc catctaaggt tataaaacct atcaaacgta
     1861 aagtatttat cgtaagttgt atctactcgt tcaatttttc ctgttccatc tgtgctaaca
     1921 attcttggta tgtattctcc aaattgccta tttatatcgg ctctaaaact tagcaacgat
     1981 ggcatataat taaagttaaa atctcttatt aaattatacc aattactttt tcttttaacc
     2041 aataattttt taaatggctc aatatatttt gatgaattat taaaagtata accaaagcca
     2101 ccacgttgtt ttgtaacatc attttttaaa actaatgggc tactttgttc agttttagtg
     2161 taggagtagc ttacatcaaa attgcttaaa ctccataatt tgtttttacc atttccttgt
     2221 acccttatgt tggtaaaatt aattgtttta atagtggttt ggtctaaggc tgtatttttt
     2281 atagaatctc tttggcgact attggcggca ctgtttattt tatcttttag ttttacatct
     2341 ttatcgtaag ggtcgtactc tgggcgttta attgttttat tgatactggc ataaattggg
     2401 atacttattt tagcagattt tggtacaagt ttaccagcat caatagaggc tgctgcatca
     2461 aactgaaaca tattatctct tgctcgttca ttaactcttt gctctatggt gccaaaacct
     2521 ttagagtaag tattggctga aaaactaagt gaaccaagat cggcaagttg tacatctatt
     2581 cttcctaaag ctgcataagc tcctctttca tctaactgag aaagccttaa ttcatttatc
     2641 caaacttccg aatataaatc aaaaggatta ttgtcttttg cattttctac tgctactaaa
     2701 attcctctta cttctcctaa attaggatta ccatataccg aataggtttt aaagccaacc
     2761 tgctctctat atattgtact taaagggctg ccagatgcat tacgccttag ctttaaattt
     2821 attaattctt gtaaactaaa atcaagtctg ttgctatctg gccacacttt tgcagcacta
     2881 ga
//
LOCUS       DAOQHP010000294         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23541_length_2880_cov_4.342301, whole genome shotgun sequence.
ACCESSION   DAOQHP010000294 DAOQHP010000000
VERSION     DAOQHP010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23541_length_2880_cov_4.342301"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLY81_08825"
     CDS             complement(<1..500)
                     /locus_tag="PLY81_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30368.1"
                     /translation="MKTINLLKGITSMLVTLLISISTYAQNTSYSSNTTPINGTNCTA
                     FGVGALTSNTSGNYNVANGGNSLFSNTTGSSNVANGYQSLYSNTTGYANSANGYQSLY
                     SNTTGSYNIANGYQSLYFNTTGGNNVANGYQSLFSNTTGYDNTANGSYSLYANTSGNN
                     NTASGR"
     gene            complement(773..2632)
                     /locus_tag="PLY81_08830"
     CDS             complement(773..2632)
                     /locus_tag="PLY81_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30369.1"
                     /translation="MKSIKTYLVLIVIHLAIKQAHSQLYVSSGANITIQDGAKITLQS
                     TNLINNSNITGAGKLILSGNSPAQISGIGNIDNLAIDNTAGISIASIVDTVRIIQSLT
                     PMSGTFTVNGNLVLVSNAVGTARIAAGTGSYINGNITVERYIPAKATRKWSFISSPVT
                     GTINNTWQQLIHITGSGTGGTVCPFLTAHSNGFDATVSNAANIFTYDASKTINTRWEV
                     PSGTLLNTVGMGKGFRVNVRGYRSIGCNLINGNPAYLSPTSVTLRATGAISNTDKNLG
                     SFSIVYPNNGINNYVFIGNPYPSPISFNAFKTTNSSSITNTYAIYIPASPSGVYSYWD
                     GVSFTGGTGYNNATGNIIASGQAFFVQSSVAGNLNLTFDEAHKTDGSDVGYFRTRNFN
                     DKIRVHYLMQDNTQLDEIIVRYAMDAAINNTDENEWDIPSINSGSHYLTSFKGYKGMV
                     VQTRNSNTLASDTVRLNVVCNQSGIYKLGFTEFEHFIGTDIFLIDHLQQSIHNVKQLP
                     VYEFTVDKDKPETKGAGRFSVVFNKLLQNNIVNYSIKMYPNPTSKMVTIELPDNSNYV
                     INIKDITGKIVLQKNTTGGANQFNISKLTSGTYLVEVTDRKRNRATEKLLVEH"
     gene            complement(2674..>2880)
                     /locus_tag="PLY81_08835"
     CDS             complement(2674..>2880)
                     /locus_tag="PLY81_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30370.1"
                     /translation="TQEVKEKGVNLAEINVKLLEKTEELTLYIIQQNKRLNEQQKHIN
                     GQQEQINNQQKQIEELKNLILNKK"
BASE COUNT          921 a          472 c          439 g         1048 t
ORIGIN      
        1 aatctaccac tagcagtatt gttattacca gatgtattag catatagtga gtagctacca
       61 ttagcagtat tgtcataccc agtagtattg gaaaatagag attggtagcc attagcaaca
      121 ttgttacctc cggttgtatt gaaatataga gattggtatc cgttagcaat attgtaactt
      181 ccggttgtat tagaataaag agattggtaa ccattagcac tattggcata tccggttgta
      241 ttggaataaa gagattggta accattagca acattgctag atcctgttgt attggagaat
      301 agtgagttgc ctccattggc aacattgtaa ttgccagatg tgttggaagt taaagcccca
      361 acaccaaatg cagtacaatt tgttccatta attggggttg tgttgctact gtaagatgta
      421 ttttgtgcat atgtacttat actaataagt aacgtaacca acatagaggt aatacccttt
      481 aataaattta ttgttttcat aatagtaatt ttatttagta aagaaatatg gtactaaagt
      541 attgtatttc ttattattaa aataataaat ataagagaaa gtgtttttgg gatatatgct
      601 taaagcctaa taaccaatgt ttatacaaag ttttattttt tataaaaaat agattgtata
      661 taaccactta ttttgttgca gtattggcaa caagaaaatg caattgaaaa taaaaaagag
      721 aagatgtttt taaacatctt ctcttttaat tttttaaaac ggtgataagt aattaatgtt
      781 ctactaacaa cttttctgtt gctctatttc tctttctatc agtaacttct accaagtaag
      841 taccactggt taatttacta atattaaatt gattggcacc accggtggtg tttttttgta
      901 gtacaatttt tccggttata tccttaatat taataacgta attagagtta tcgggcagtt
      961 ctattgtaac cattttactg gtagggtttg gatacatttt aatagaataa tttacaatat
     1021 tattttgtaa tagtttatta aaaactacac taaatctgcc tgctcccttt gtttcaggtt
     1081 tgtctttatc tacagtaaac tcataaacag gcaactgttt tacgttgtga atagattgtt
     1141 gtaaatggtc tattaaaaaa atatcggtac caataaaatg ttcaaattcg gtaaacccta
     1201 atttataaat tccgctttga ttgcaaacaa catttagcct aacagtatcg ctagcaagtg
     1261 tattactatt tctagtttga accaccatgc ctttatatcc tttaaagctt gttaaataat
     1321 ggctgccact gttaatagaa ggaatgtccc attcgttttc atctgtatta tttatagctg
     1381 catccattgc ataacgaaca attatttcgt ctaactgtgt attatcctgc attaaataat
     1441 gcactcttat tttatcatta aaatttctgg tacgaaaata accaacatca gacccatctg
     1501 ttttatgtgc ctcgtcgaaa gttaagttta aattacctgc aacagaagac tgcacaaaaa
     1561 atgcctgacc actggcaata atatttcctg tagcattatt ataacccgta cctcctgtaa
     1621 aagaaacacc atcccaatag ctatacaccc cactagggct ggcaggtata taaatagcat
     1681 aagtattagt tatactactg ctattggtag ttttaaatgc attaaaacta atagggctgg
     1741 ggtaagggtt gccaataaaa acataattat taataccatt gttagggtaa acaatactaa
     1801 agctacctaa gtttttatct gtattactga tagccccagt agccctaagt gtaacacttg
     1861 tagggcttaa atatgccggg ttaccattta ttaaattgca acctatagat ctatacccac
     1921 gaacatttac tctaaatcct ttacccatac ctacagtatt caataaagta ccagaaggta
     1981 cctcccaacg ggtattaatt gttttactag catcataagt aaaaatattt gcagcattgc
     2041 ttaccgttgc atcaaaacca ttgctatgtg cagttaaaaa aggacaaaca gtaccacctg
     2101 tacccgaacc tgtaatatgt attagttgtt gccatgtatt atttattgta ccagtaacag
     2161 ggctgctaat aaaactccat ttacgagttg ccttagctgg tatataacgt tcaacagtaa
     2221 tattaccgtt aatataacta cctgtacctg ctgctattct ggctgttcca acagcattgc
     2281 ttactaatac taaattgcca ttaacagtaa aagtacccga catgggcgtt aaagactgta
     2341 taattcttac agtatctaca atactggcta tactaatacc tgcagtatta tctattgcca
     2401 aattatctat attgccaatg ccacttattt gagcagggct attgccagaa agaattaatt
     2461 ttcctgcacc agttatatta ctattgttaa taaggtttgt actttgaaga gttatttttg
     2521 caccatcttg tatggtaata tttgctccac tactaacata caattggctg tgagcttgtt
     2581 taatagccaa atgaattact attaaaacaa gatatgtttt tatacttttc attggtttat
     2641 ttattaaatt ttaaattata taaaagtaac aatttatttt ttatttaata ttaagttttt
     2701 tagttcctct atttgtttct gttggttatt aatttgttcc tgttggccat taatatgttt
     2761 ctgttgttcg tttaaccttt tgttttgttg aataatgtat aaagtaagtt cttctgtttt
     2821 ttctaaaagt tttacattta tttcagcaag gttaacaccc ttctctttta cttcctgtgt
//
LOCUS       DAOQHP010000295         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_23838_length_2857_cov_3.497859, whole genome shotgun sequence.
ACCESSION   DAOQHP010000295 DAOQHP010000000
VERSION     DAOQHP010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23838_length_2857_cov_3.497859"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="PLY81_08840"
     CDS             complement(<1..2359)
                     /locus_tag="PLY81_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30371.1"
                     /translation="MLLLVAAILYLVSFIVTLFYNYANNPTRVQEKLSKSIQKKERSF
                     EGFLANKQIIESIIYDTLNNTNKNNLQEYNYGIYIYKQKQQPELIYWNNNNYSIAKSD
                     ILKNDNTFIVRYQNGIFECIKQTINIHNNQFIVAALLPIHRHFFIENKYLKSAFDEYP
                     EFDDYYEITNNSNGYSITNTKGNSIYTISKKAGANNTNYNLITILLRIAAIVCLFFYL
                     HFLCLKTLQKKGFNKAFLLLSTVLILLRTISYFINFPFNFSQLPLFDPSVYASNFLHP
                     SLGDLLINCILIFWLIIFYKVNCTLKNSALLFIIRNKKLQKIVPYIHLVALVIITFLC
                     TSTIKSLVLDSKVSLDVTNFFSLTWFSIVSFIILCFCVLIFYHLSHLLLNAVFNFQIS
                     LFKQLLIIALAGLALLSNQLISVSATSNLLTILWLLIYVVIINFRKVDTSIYLLHSTF
                     FIFWILFFSINVAALLMLQNNYIETEQRKKWAEELAEKIDPNSETLLKIATTNFNDEF
                     LQHNFHRFQEEFSNKFIKDSLVNENFSGYLNKYDTRIYTFDCAFNPLFNDDSLPFSVI
                     RTIVLNQSKSTTIVNLHSFEDAYQKENYLYEKNIVADSGTLGYLFVIVKPKQYKSEAI
                     YPELFKQVKDLSNDFNIHYAYAIYNNGALINSFNNYNFPFAHHVSKTPSGKYKIIKQG
                     SYKELWYSNQSGKTVVIVKKSSTLLELFTLFAYLFCTFLILVALLHIASFLIKTRLKK
                     EAIKQVLQFKISTQIQAIIIMISLFSFIVIGVTTISFFIYRFNQTN"
     gene            complement(2395..>2857)
                     /locus_tag="PLY81_08845"
     CDS             complement(2395..>2857)
                     /locus_tag="PLY81_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HNF30372.1"
                     /translation="KVESEEDVKRNWEKIKLNEGKKKSVLSGVPTGLPAIIKASRMQE
                     KAKQVGFEWENKNDVWKKVEEEMCELQEAIKHNNQQHIEEEFGDVLFSLINYARFLKI
                     DAEGALEKTNQKFMHRFKSMEEIATAKGKSLTNMSLEEMDSIWNEIKKANN"
BASE COUNT         1015 a          384 c          372 g         1086 t
ORIGIN      
        1 gatttgtttg attaaaacga taaataaaaa atgaaatggt agtaacgcct ataacaataa
       61 atgaaaacaa actaatcatt ataattatag cttgaatttg tgtactaatt ttaaattgta
      121 atacctgttt aattgcttct ttttttaatc ttgttttaat taaaaaactt gcaatatgca
      181 gcagagccac taaaattaaa aaggtgcaaa ataaataggc aaataaagta aacaactcta
      241 gcagtgttga gctcttttta acaataacaa cagtttttcc cgattggtta ctataccaca
      301 attctttata agaaccttgc ttaattattt tatattttcc tgaaggagtt tttgaaacat
      361 gatgtgcaaa tggaaaatta taattattga agctatttat taaagctcca ttattataaa
      421 ttgcataagc ataatgaata ttaaaatcgt tggataaatc tttaacctgt ttaaataatt
      481 caggatatat tgcttcgctt ttatattgtt ttggtttaac aataacaaat aaatatccaa
      541 gtgttccact atctgctaca atattttttt catatagata attttctttt tgataggcat
      601 cttcaaaact atgtaagtta acaatagtag tgcttttgct ttgatttaga acaattgttc
      661 taataacaga aaagggtaga gaatcatcat taaacaaagg attaaaagca cagtcaaaag
      721 tataaattct tgtatcgtat ttgtttaagt aaccagaaaa gttctcattc accaaactat
      781 ctttaataaa tttattcgaa aactcttctt gaaatctatg aaagttgtgt tgcaaaaatt
      841 cgtcattaaa atttgtagtt gcaattttta aaagtgtttc actattaggg tctatttttt
      901 ctgctaattc ctcggcccat ttttttcttt gttctgtttc tatataattg ttttgtaaca
      961 ttaataatgc tgcaacattt attgaaaaaa ataatatcca gaatataaag aatgtagaat
     1021 gtagtaaata aatactagta tctactttgc gaaaattaat aataactaca taaataagta
     1081 accataaaat agtaagcaaa ttagacgttg ctgaaactga tattaattgg ttgcttagta
     1141 gtgccaaacc tgccaaagca ataatcaaca attgtttaaa caaagaaatt tgaaaattaa
     1201 aaacagcatt aagtaataag tgagataagt gataaaaaat taatacgcaa aaacataaaa
     1261 taataaagct tactatgcta aaccaagtta aactaaaaaa gttggtaaca tctaatgata
     1321 cttttgaatc taataccaaa cttttaatgg tactagtaca caaaaaagta attattacaa
     1381 gtgccactaa atgaatgtaa ggaacaattt tttgtaactt tttatttctg ataatgaata
     1441 ataaagccga gttttttagt gtgcaattaa ctttataaaa aataatcagc cagaaaatta
     1501 aaatacaatt aataagtaaa tcgcctaagg aaggatgtaa aaagtttgaa gcatatacag
     1561 atggatcaaa aagtggtaat tgactaaaat taaatggaaa attgatgaaa taggatattg
     1621 ttctaagcaa tattaaaaca gtggataaaa gtaagaatgc tttattaaat cctttttttt
     1681 gaagagtttt taaacacaaa aagtgcaagt aaaaaaacaa acatacaatg gcagcaattc
     1741 gtaataatat agtaattaaa ttataattag tattgttggc acctgctttt ttagaaatag
     1801 tataaattga attgcctttt gtatttgtta tactgtaacc attgctatta ttggttattt
     1861 cgtaataatc atcaaactca ggatactcat caaaagcact ttttaaatat ttattttcaa
     1921 taaaaaagtg cctgtgaatg ggtaataagg cagcaacaat aaattggtta ttatgaatat
     1981 taattgtttg ttttatacat tcaaaaatgc cattttggta acgtacaata aatgtgttat
     2041 cgttttttag aatgtctgat tttgcaatgg agtaattatt gttattccaa tatattagct
     2101 ctggttgttg tttttgtttg tatatataaa tgccatagtt gtactcctgt aaattatttt
     2161 tattggtgtt gtttaaagta tcgtaaatga tgctttcaat tatttgttta ttcgctaaaa
     2221 aaccttcaaa gcttctttcc tttttttgaa tagatttact tagtttctct tgtactcttg
     2281 tagggttatt ggcatagtta taaaaaaggg taactataaa agatactaga tataatattg
     2341 ctgctactaa taacaacaat attttttttt gatgtataag tttggttaag ttgctcaatt
     2401 atttgctttt tttatttcat tccaaatact atccatttct tctaaactca tattggttaa
     2461 tgattttcct ttggctgttg caatttcttc catgctttta aacctatgca taaatttttg
     2521 attggttttt tctaaagcac cttctgcatc tatcttcaaa aaccgtgcat aatttattaa
     2581 gctaaaaagt acatcgccaa attcttcttc tatatgctgc tgattattat gcttaattgc
     2641 ctcttgtaat tcgcacattt cttcctccac ttttttccaa acatcatttt tattttccca
     2701 ttcaaatcca acttgtttgg ctttttcttg cattctgctt gctttaatta tagcaggcaa
     2761 gccagtaggc acaccactta atacgctttt ctttttccct tcattaagtt ttattttctc
     2821 ccagtttctt tttacatctt cttcactttc tactttt
//
LOCUS       DAOQHP010000296         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24035_length_2843_cov_3.361191, whole genome shotgun sequence.
ACCESSION   DAOQHP010000296 DAOQHP010000000
VERSION     DAOQHP010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24035_length_2843_cov_3.361191"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLY81_08850"
     CDS             <1..389
                     /locus_tag="PLY81_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNF30373.1"
                     /translation="KKNDSVYSKRIRAGKRRTYFFDVRATRGNDYFLTITESRKRFDD
                     NGYDRHKIFLYKEDFNKFIKALGEAVDYVKTDLMPDFDFDAFNHEYPENENGEQDSRT
                     ITDPVVEPIASNIEAQNNSTEEVDKW"
     gene            514..1212
                     /gene="truB"
                     /locus_tag="PLY81_08855"
     CDS             514..1212
                     /gene="truB"
                     /locus_tag="PLY81_08855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNF30374.1"
                     /translation="MQPIQKFLDGQVLLFYKPLKWTSFDVVKKIRSLIKVSKIGHAGT
                     LDPLATGLLIICTGKFTKKINEFMGQPKTYTGSFTLGATTSSYDLEQTPENFKDISHL
                     TATEIYNATLPFIGNILQMPPQHSAIKKDGKRLYEMARKGIEVKVEPRPVTIYTFEIT
                     KIELPLVYFKVICSTGTYIRSLANDFGNALNTGAYLSSLCRTDIGEFNIENAYTIESF
                     ENEIAKLRNSETTI"
     gene            1215..2771
                     /locus_tag="PLY81_08860"
     CDS             1215..2771
                     /locus_tag="PLY81_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30375.1"
                     /translation="MNLVKYITNYFSSKNRIFLVCILTIHVIFSYYFISKQYITYDEP
                     SYIEYSKRWLHGKPERVEALDDSKTPVISIVWLPRIVQQIIQPNLKQTDFGKKDQEQG
                     RYMMIIFSLLLLIYFYKFLQLLKIKYWLPVFLFIIIDPLLIAYSVLINSDLLSGLVLL
                     AIIYHLYQYLFFKKRSHFIYSCIWLATGLVTKLSFVFFIPVFWLIIFVVHKKIDWRKF
                     LLFVLLILLVVNTWFYFKNTCLPLGQFQFSSNTFKLVQQFLHFLHWLPLPLPENYIYS
                     IDLLQYHAQLGGSYNNTYPGVYILNKFSLTNNFWYYYLVAAWYKFPISILFLITTGLF
                     AFLYRFKKNIQKYWIIIISIVYYLLVLSCFNKFQIGIRHLLIVLPLMYILIAVLLNYL
                     VQTKFKWGIAILWLFMFFSVAKYYPNLIPYTNELVTNKAKVYEKLMDTSIDYGQSKLD
                     ASNFLQQNTDFKTLTETPQTGKYLVSMQQLVLHQMYKDTSYNWLIKNYEPIGMYKYVY
                     LQFKIDALKR"
BASE COUNT         1022 a          371 c          417 g         1033 t
ORIGIN      
        1 aaaaaaagaa tgacagcgtt tatagtaaac gtattagagc aggaaaaaga agaacttact
       61 tttttgatgt aagagcaact agaggaaatg attacttttt aacaattacc gagagtagaa
      121 aacgttttga tgacaatggc tacgacagac acaaaatttt tctttataaa gaagatttca
      181 acaaatttat taaagcttta ggcgaagctg tagattatgt aaaaacagat ttaatgcctg
      241 attttgattt cgatgctttc aaccatgagt atcctgaaaa tgaaaatgga gaacaagact
      301 caagaactat tactgatcca gttgttgaac ctatcgcatc taacattgag gctcaaaata
      361 attctacaga agaagtagat aaatggtaat ttaagtacaa caaataataa ataaaaagcc
      421 ttgctaaata aatactagca aggttttttt aaccctacta ataagtattt ctatacttag
      481 ttcaataatt tactttctta actttgatga ataatgcaac ccattcaaaa atttttagac
      541 ggacaggttt tactttttta taaaccatta aaatggacaa gttttgatgt agtaaaaaaa
      601 attcgttcac ttattaaagt atctaaaatt ggtcatgcag gcactttaga ccctttagct
      661 acagggcttt taattatttg tacaggaaaa tttacaaaaa aaataaatga atttatgggg
      721 cagcctaaaa cctacactgg tagttttact ttaggtgcta caacaagctc ttatgattta
      781 gaacaaacac cagaaaattt taaagatatt tctcacttaa ctgcaacaga aatttataat
      841 gccactttac catttattgg taatatttta caaatgccac cacagcactc tgcaattaaa
      901 aaagatggta aaagattata cgaaatggcc cgaaagggaa ttgaagtaaa ggtagaacca
      961 agacctgtta caatttatac atttgaaatt acaaaaatag aactgccatt ggtgtatttt
     1021 aaagttattt gtagtactgg cacttatata agaagccttg cgaatgactt tggtaatgct
     1081 ttaaatactg gtgcttattt aagtagtttg tgtagaacag atataggtga atttaatatt
     1141 gaaaatgcct acacaattga aagttttgaa aatgaaatag caaagcttag aaattctgaa
     1201 acaaccattt aaatatgaat ttggtaaaat atataaccaa ttatttttcg agtaaaaaca
     1261 gaattttttt ggtttgtatt cttactattc atgttatctt ttcttattac tttataagca
     1321 aacaatatat tacttatgat gaaccaagtt atattgagta tagtaaacga tggttacacg
     1381 gtaaaccaga aagagtagaa gctttagatg atagtaaaac accagtgatt agtattgtat
     1441 ggctgccacg tattgttcag caaattattc aacctaactt aaagcaaaca gattttggta
     1501 aaaaagatca ggaacaaggc aggtacatga tgataatatt ttcgctactg ctacttattt
     1561 atttttataa atttcttcaa cttcttaaaa taaaatattg gctacctgtt tttttattta
     1621 ttattattga tcccctgctt attgcatatt ctgtattaat aaactccgat ttattaagtg
     1681 gattagtttt acttgctatt atatatcatt tgtaccaata tttatttttt aaaaaaagaa
     1741 gccattttat ttatagttgt atttggttag ctacaggctt ggtaactaaa ttgagctttg
     1801 ttttttttat tcctgtattt tggctaataa tatttgtagt tcataaaaaa atagattgga
     1861 gaaaattttt actttttgtt ttacttattt tacttgttgt taatacttgg ttttatttta
     1921 aaaacacttg tttaccttta ggtcagtttc aatttagcag caatactttt aaattagttc
     1981 aacagttttt acactttttg cattggctac ctttaccact gcccgaaaat tatatttata
     2041 gtatagattt gttgcaatac catgcacaat taggaggtag ttacaacaac acatatccag
     2101 gtgtttatat actcaacaaa ttttcactta caaataattt ttggtattat tatttagtgg
     2161 cagcttggta taaatttcct attagtattt tatttttaat aacaactggt ttatttgctt
     2221 ttctttatag gtttaaaaaa aatattcaaa agtattggat aattattatc tctattgttt
     2281 actatttatt agtattaagt tgctttaata aatttcaaat tggtataagg catttgctaa
     2341 ttgttttacc tttaatgtat attttaattg cagtattatt aaattactta gtgcaaacaa
     2401 agttcaagtg gggtattgca atattgtggc tttttatgtt tttttctgtt gcaaaatatt
     2461 acccaaactt aattccatat actaatgaat tggttaccaa taaagccaaa gtatatgaga
     2521 aattaatgga tacaagtatt gattatgggc aaagcaaatt agatgcaagt aattttttgc
     2581 aacaaaacac agattttaaa acactaacag aaacaccaca aacaggaaag tatttagtaa
     2641 gtatgcaaca acttgttttg caccaaatgt ataaggacac ttcatacaat tggctcataa
     2701 aaaattatga gccaattggg atgtataaat atgtttattt acaatttaaa atagatgcat
     2761 taaaaaggta aatcatctac caaatcatcg gtattattag ttggtgtaaa ttcgttggta
     2821 ttgcttgttg tattttctac agt
//
LOCUS       DAOQHP010000297         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24110_length_2838_cov_3.210564, whole genome shotgun sequence.
ACCESSION   DAOQHP010000297 DAOQHP010000000
VERSION     DAOQHP010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24110_length_2838_cov_3.210564"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLY81_08865"
     CDS             complement(<1..897)
                     /locus_tag="PLY81_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNF30376.1"
                     /translation="MEITPGKLLQNINSPDDLKKISREELHQVCSELRQYIIDVVSVH
                     GGHFGASLGVVELSVALHYIYNTPYDQLVWDVGHQAYGHKILTGRRDNFITNRKYKGL
                     SGFPKRSESEYDTFGVGHSSTSISAALGMAMASKYKGENRKSIAVIGDGSMTAGMAFE
                     AMNHAGIADADMLVILNDNCMSIDPNVGALKEYLTDIATSPTYNKVRDDIWKLLGKLP
                     IGKTFTREMASKLEHAVKGVVNKSSNLFEALNFRYFGPIDGHNITKLVDTLKDLKNIP
                     GPKLLHIVTVKGKGYALAEKDQT"
     gene            1085..1804
                     /locus_tag="PLY81_08870"
     CDS             1085..1804
                     /locus_tag="PLY81_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF30377.1"
                     /translation="MNVQLFVPCFIDQLYPNVAFNMLKVLEKAGCIVRYNSNQTCCGQ
                     PAFNAGFWGEARDVCQKFLEDFSHEGYIVAPSASCIGFLKNYYSKIFEGTQYLEKVKN
                     LSDRAFEFSDFMINVLNIDDVGAIFNEKVTYHDSCAALRECKIKTEPRRLLSKVKGLE
                     LVEMNDVETCCGFGGTFAVKFEAISIAMADQKITNAEATKADYIVSTDMSCLMHIDGC
                     IQHKHKNLKVIHLADVLASGW"
     gene            1831..2235
                     /locus_tag="PLY81_08875"
     CDS             1831..2235
                     /locus_tag="PLY81_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNF30378.1"
                     /translation="MNYWLVKSEPFKYSWQQFEKEGQTFWDGVRNYAARNNLKAMKKG
                     DLVFWYHSNEGLEIVGIAQVIKEAYQDPTTEDEAWVVVDLKPYKKLKKPVSLAQIKAE
                     KTLANMDLVRLSRLSVGTVRKQEWEKIIEMSK"
     gene            2329..>2838
                     /locus_tag="PLY81_08880"
     CDS             2329..>2838
                     /locus_tag="PLY81_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30379.1"
                     /translation="MKKGSIVLLLMSFVFSLHAQNIEGSWYGIGKAETEYSEGNTYLT
                     ELSLTQNGRNITGQLNFYFRDSLFSNTVTGTYDHATRALVFKPTKIIYYKSNNTLTGV
                     DCEVEAYFSLRFSRVESVLTGAFYATNNFKYTCPTINLKLKKNDVVVQEEEVISTIYE
                     EEPTTPKELI"
BASE COUNT          968 a          406 c          494 g          970 t
ORIGIN      
        1 agtttgatct ttttctgcca atgcatagcc tttgcctttt acagttacaa tatgtaatag
       61 tttaggacca ggaatgtttt ttaaatcctt taatgtatcc accaacttag taatattatg
      121 gccatcaatt ggtccaaaat atctaaagtt taaagcttca aataagttac tacttttatt
      181 tactacacct tttacagcat gctctagttt gcttgccatt tctctggtaa aagttttacc
      241 aattggcaat ttgcctaata gtttccatat atcatctcta actttattgt atgttgggct
      301 ggttgcaata tcagttaaat attcttttaa tgcccctaca ttggggtcaa tgctcataca
      361 gttatcgttt aaaataacca acatatctgc atctgcaata cctgcatggt tcattgcttc
      421 aaaagccatt ccagcagtca tacttccatc gccaattaca gcaatacttt tcctgttttc
      481 tcctttatat ttcgaagcca ttgccatacc taaggcagca gaaattgaag tagaagagtg
      541 gcccacacca aaagtatcat actcactttc actacgttta ggaaaaccac ttaagccttt
      601 gtatttacgg ttggtaataa aattatctct tctgccagta agtattttat gaccataagc
      661 ctgatggcca acatcccaaa ccaattgatc gtaaggtgta ttataaatgt aatgtagtgc
      721 aacagataat tcaaccactc ctaaacttgc accaaaatgc ccaccatgca cacttactac
      781 atcaataatg tattgtctta actcactaca aacctgatgt aattcttctc tgcttatttt
      841 ctttaaatca tcagggctat taatattttg taataatttt ccgggagtaa tctccatttt
      901 tataaattat ttgtgctgta aaaatagtgt ttacggcttt tatttacaac attcagttat
      961 acatattaat tttaaaagat tttaaaacta atttctgagg tacagagtta atgcttaaaa
     1021 aagatacttt tgtaaactgc ggctcaattt ttttaaaaaa aagtagatta gtaaataagt
     1081 ttatatgaat gtacaattat ttgtgccttg ctttattgat caattatacc caaacgttgc
     1141 ttttaatatg ctaaaagtgt tggagaaagc aggctgcatt gttaggtata acagtaatca
     1201 aacctgttgt gggcaaccag cttttaatgc aggtttttgg ggcgaagcaa gagatgtttg
     1261 ccaaaaattt ttagaagact tttctcacga aggttatatt gtagcaccaa gtgcaagttg
     1321 tattggtttt ttaaaaaatt attattctaa aatttttgaa ggtacacagt acttagaaaa
     1381 agtaaaaaac ttatctgata gagcttttga gtttagtgat tttatgatta atgttttaaa
     1441 tatagatgat gtaggtgcta tatttaatga gaaagtaact tatcatgata gttgtgctgc
     1501 tttaagggaa tgtaaaatta aaacagaacc aagaaggttg ctaagtaaag taaaaggatt
     1561 agaattggtt gaaatgaatg atgtagaaac ttgctgtggc tttggtggca catttgctgt
     1621 aaaatttgaa gcaattagta ttgctatggc agatcagaaa ataaccaatg cagaagcaac
     1681 aaaggcagat tatattgtta gtacagatat gagttgttta atgcatattg atggctgtat
     1741 tcaacataaa cataaaaact taaaagtaat tcaccttgca gatgttttag cttcggggtg
     1801 gtaatttttt tataatctaa tttttttaag atgaattatt ggttagttaa atcggagcca
     1861 tttaaataca gttggcaaca gtttgaaaaa gagggtcaaa cattttggga tggtgtaaga
     1921 aattatgctg caagaaataa tttaaaagcc atgaaaaaag gcgatttagt tttttggtat
     1981 catagcaacg aggggcttga aattgttggt attgcacagg ttataaaaga agcttatcaa
     2041 gacccaacca cagaggacga agcctgggtt gtagttgatt taaaaccgta taaaaaatta
     2101 aaaaaacctg ttagccttgc tcaaattaag gctgaaaaaa ctttagcaaa tatggatttg
     2161 gtgaggctta gcagattatc tgttggtact gtaagaaaac aagagtggga aaagattatt
     2221 gaaatgagca aataaggtgt tttttaaagc aaataattca cattaaaagg caatctgtta
     2281 gcaactttta actttgttta tacaaactga ttatgttctt tgcagaatat gaaaaaaggg
     2341 agtattgttt tgttgttaat gagttttgta tttagcctac atgcacaaaa tattgaaggt
     2401 agttggtatg gcattggaaa ggctgaaact gaatacagcg aaggaaatac ttatttaact
     2461 gaattgagtt taacacaaaa tgggagaaat ataacagggc aattgaattt ttattttaga
     2521 gatagccttt tttctaatac tgttacaggt acttacgacc atgctacaag agcactggtt
     2581 tttaaaccta caaaaattat ttactataaa tctaataata ctttaacagg ggttgattgt
     2641 gaagtagagg catatttttc tttacgtttt tctagagtag aatcggtatt aacaggggct
     2701 ttttatgcta ctaataattt taaatatact tgccctacta ttaatttaaa attaaaaaag
     2761 aatgatgttg tagttcaaga agaagaagtt atctcaacta tatatgaaga ggaaccaact
     2821 acaccaaagg aattaatt
//
LOCUS       DAOQHP010000298         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24314_length_2824_cov_4.077284, whole genome shotgun sequence.
ACCESSION   DAOQHP010000298 DAOQHP010000000
VERSION     DAOQHP010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24314_length_2824_cov_4.077284"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLY81_08885"
     CDS             complement(<1..628)
                     /locus_tag="PLY81_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNF30380.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDNFLAFDAQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIARGVIRKIGYTKSEYMFEANSCGILSAIHEQSADI
                     NQGVDRKKKEEQGAGDQGMMFGYANNETEDYMPLALDLAHKILIELAALRRENKQIKY
                     LRPDAKSQVTLEYDDNNKPVRIDAIVVSTQHDDFDTEAKMLAKIKKDII"
     gene            complement(749..2194)
                     /locus_tag="PLY81_08890"
     CDS             complement(749..2194)
                     /locus_tag="PLY81_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNF30381.1"
                     /translation="MKNLLSVFFISIIITSCTKISTTDIGNNLIPPIDGINTLDTIID
                     VQSFNVLADSFRISKYQEMVLGNIDNDPLMGSTKAIINAVFKPSYFPFYFPVSKDSLT
                     LDSVVLVLSYQGLYGDTTNPVHLKVFEISQTSKLKADTVYPVSATMNTAGLLGEKIIS
                     DPRKLADSVKAFSENAKNQIRIPLNNSFGNKLLKQFDSSNAYFSDDQFESIFRGFSII
                     PQAGSNTLLRVALNDTNSKVALYYKYKQRVTGVDTSVITYYRPAGITSGASNNIIRNR
                     TGSQSAQYLTNASNVEDSLLFLQAGLGNYVRIKTPGITGLSNRVIHRAELIMEQEAND
                     VNTDGLFTTPNLFLCAISTHPDSAAYRFYIPKDIVTSGADGLVENLALFGGNAVNATD
                     PNGKKISSYNFNITRYIQSIILGKQSVYDLYLFAPVVDYVYAGYGNTAGVYQISSIPL
                     NSPGVGRVRLLGGNSKQHGRKMRLRLVYSTL"
     gene            complement(2228..>2824)
                     /locus_tag="PLY81_08895"
     CDS             complement(2228..>2824)
                     /locus_tag="PLY81_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF30382.1"
                     /translation="DYPLQIKVASLPNARLQIYFLENEEFFKRKNIFHDDEDKWFEDN
                     ALRTVFFAKGALETVRKFGWPPDIIHCSGWMSGLIPAFIKTAYKREPVFLHSKTIFTI
                     GQNTFEEKIGEELLELALINNSITANDLKAFKDNDNAAMFRGGATFADAITFGTPDPD
                     VKLGEEFAKVRGKKVLPFKGWDTDLTEYLELYNDLAGK"
BASE COUNT          928 a          496 c          396 g         1004 t
ORIGIN      
        1 caataatatc ttttttaatt ttagctaaca ttttagcttc tgtatcaaaa tcatcgtgtt
       61 gtgtagatac tacaatggca tcaattctaa ctggtttatt attatcatcg tattctaaag
      121 taacctggct ttttgcatcg ggacgtaaat atttaatttg cttgttttct cttcttaatg
      181 ctgctaattc aatcaaaatt ttatgtgcta aatctaaggc caatggcata taatcttctg
      241 tttcattatt ggcataacca aacatcatac cctggtctcc tgcaccttgt tcttctttct
      301 tttttctatc aacaccttgg ttaatatctg cactttgttc gtgaatagca gaaagaatac
      361 cacaagagtt ggcttcaaac atatattcgc ttttagtgta accaattttt ctaattacac
      421 cacgagcaat ttcttgtaca tctaaataag ctttagattt tacttcacca gcaagtacaa
      481 cctgaccagt ggttacaaga gtttcacaag caactttgct ttgggcatca aaagcaagaa
      541 aattgtcaat taatgcatca gatatttggt ctgctacttt atctggatgg ccttcagaaa
      601 cactttcaga agtaaataaa taagacatgt attataattt tatttttaat gattgcaaat
      661 ataaaggttg attttatttt aactaaaaag tatttagtac atataattaa atagatttta
      721 atctatacaa aataaaatca attgaaggct acagagtaga atagactaac cttaatctca
      781 tttttcttcc atgttgttta ctgttccctc ctaataatct aaccctgcca acacctggtg
      841 aatttaatgg tattgaagat atttgataaa ccccagcagt attaccatat ccagcataca
      901 cataatccac tacaggagca aacaaataca aatcgtaaac agattgtttt cctaaaataa
      961 tactttgaat atatctggta atattaaaat tatacgatga aattttcttc ccattcggat
     1021 cagtagcatt tactgcatta cctccaaata aagcaagatt ttcaaccaat ccatctgcac
     1081 cagaagtaac aatatctttt ggaatataaa atctataagc agcagaatct gggtgtgtgc
     1141 taatggcaca taaaaataaa ttaggagttg taaatagccc atctgtgttt acatcattgg
     1201 cttcttgttc cataattaat tctgctctat gaataaccct attactcaag cctgttatac
     1261 caggtgtttt aattctaaca taatttccta aacctgcttg taaaaacaat aaactatctt
     1321 ctacattgga agcattagtt aaatattgtg ccgattgtga gcctgttcta tttctaataa
     1381 tattattact tgcaccagat gtaattcctg caggcctgta ataagtaatt acacttgtat
     1441 caacccctgt aactctttgt ttgtatttat agtacaatgc tactttacta tttgtatcat
     1501 ttaaagctac tcttaaaaga gtattgctac cagcttgtgg tattattgaa aagcctctaa
     1561 aaatactttc aaattggtca tcactaaaat atgcattact actatcaaat tgctttaaga
     1621 gcttattacc aaacgaatta tttaaaggaa ttcggatttg attttttgca ttttcgctaa
     1681 atgcttttac tgaatctgca agtttacggg ggtctgagat gattttttca cctaataatc
     1741 cagcagtatt catagttgca gaaacagggt aaactgtgtc tgcctttaat ttacttgttt
     1801 ggcttatttc aaatactttt aagtgaacag ggttagttgt atcgccatat aagccttgat
     1861 aacttaatac taatacaacc gagtctagag ttaaactatc cttacttacc ggaaaataaa
     1921 aaggaaaata ggaaggctta aacactgcat taattatggc ttttgtacta cccatcaatg
     1981 ggtcattatc aatattacct aaaaccattt cttggtactt agaaattcga aaggaatcgg
     2041 ctaaaacatt aaaactttgt acatcaataa tagtgtctaa agtattaatt ccatcaatgg
     2101 gtggaattaa attattacct atatcggttg tagaaatttt tgtacaagag gtaatgatga
     2161 tgcttataaa aaaaacggaa agtaaattct tcacgcagta gaatattaaa gtaaaatgtt
     2221 gattttgtta tttacctgca aggtcgttgt acaattctaa atactctgtt aaatcagtat
     2281 cccatccttt aaaaggtaat actttttttc cacgtacttt ggcaaactcc tctcctaatt
     2341 tcacgtcagg gtctggtgtt ccaaaagtaa ttgcatcggc aaatgttgct cctcctctaa
     2401 acatggcagc attatcattg tctttaaaag cttttaaatc gtttgcagtt atgctattgt
     2461 taattaatgc taattctaaa agttcctctc ctattttctc ttcaaaagta ttttggccaa
     2521 ttgtaaaaat ggttttacta tgtaaaaaaa caggttctct tttataggct gttttaataa
     2581 aggcaggtat taagccactc atccaaccac tacaatgaat tatatcagga ggccaaccaa
     2641 attttcgtac agtttctaaa gcacctttag caaaaaaaac tgttcttaaa gcattatctt
     2701 caaaccattt gtcttcatcg tcatgaaata tattttttct tttaaaaaac tcttcgtttt
     2761 ctaaaaaata tatttgaagc ctggcattcg gcaacgaggc tactttaatt tgtaaaggat
     2821 aatc
//
LOCUS       DAOQHP010000299         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24360_length_2821_cov_3.996385, whole genome shotgun sequence.
ACCESSION   DAOQHP010000299 DAOQHP010000000
VERSION     DAOQHP010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24360_length_2821_cov_3.996385"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..869)
                     /locus_tag="PLY81_08900"
     CDS             complement(210..869)
                     /locus_tag="PLY81_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNF30383.1"
                     /translation="MKTTLLSILTLMLFAEATAQIPKEKTLLWKIEGNSINQPSYLFG
                     TFHMLCSNDFEMPDTIKSLMGMCKQLFLEIDIDDPQMTTTMMKHIKMKDGKRLKDFLT
                     QEEYDTATVLFKKVVGMNLKMVETYKPFMLTSMLYPKMMGCNIIAFEKELAKLAKNND
                     YETKGLETIEDQLAVFDDVSYKIQAKMFLKSLLEIEEGKTQIQKIVTLYNSKNIRAMQ
                     R"
     gene            complement(947..2293)
                     /gene="purB"
                     /locus_tag="PLY81_08905"
     CDS             complement(947..2293)
                     /gene="purB"
                     /locus_tag="PLY81_08905"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNF30384.1"
                     /translation="MELNNLTAISPIDGRYRKQLAFLDDYFSEFALIKYRVAIEIDYF
                     LFLAEKGVFKINSTNKQRLLKVKENFSIEDANKIKNIEAITNHDVKAVEYFIKEELDK
                     ENLGNLKEWVHFGLTSQDINNTAIPLSWKHCAENEYLPMIANTHNLLVRMARKWKSVT
                     MLARTHGQPASPTILGKEIMVFAERIEGQIMQFSYIPFSAKFGGATGNFNAHVVAFPN
                     KKWIAFGNEFVDKILGLQRMQFTTQIEHYDNLAAHLDCIKRINTILIDLCRDIWTYIS
                     LDYFKQKTKKGEVGSSAMPHKVNPIDFENAEGNLGVANALLEHLSAKLPISRLQRDLT
                     DSTVLRNLGVPIAHTLLAIKSIEKGLGKLLLNENKINADLDNNWAVVAEAIQTILRKE
                     NYPNPYEALKELTRGKSSINQQSIHLFIDSLKINLELKKELKKITPYNYTGVQANY"
     gene            2369..>2821
                     /locus_tag="PLY81_08910"
     CDS             2369..>2821
                     /locus_tag="PLY81_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30385.1"
                     /translation="MNKIKIGAVSYLNTKPLLYGIKHSAIINNIELIEDHPANIAELL
                     LANKIDIGLVPTAIIPQISNATIVADYCIGANATVASVCLFSETTIDNIEAVILDYQS
                     RTSVALCKILLKEYWKREVKFIEGREGYRNEIKGTTAAIVIGDRAFEQA"
BASE COUNT          895 a          443 c          414 g         1069 t
ORIGIN      
        1 cctctgtagc ttgaagtaaa gaatcgaaat cgcttgatcg ttgatctctt tcagaatttg
       61 agccatcact tccttggtcc catttcgagg gatccgagtc tccccgacga aactcttcaa
      121 aatactacaa atatgcgtta gagtttcatt tctttcaaag taatccccat ttaggttagc
      181 ttttacactc ctccgatttc tatatacatc tatctttgca ttgctctaat atttttagaa
      241 ttatacaagg taacgatttt ttgtatttgt gtttttcctt cttctatttc taataaagat
      301 tttaaaaaca ttttagcttg tattttataa gatacatcat caaaaactgc taactggtct
      361 tcaatagttt ccaatccctt tgtttcatag tcattgtttt ttgcaagctt agctaattcc
      421 ttttcaaaag caattatatt acaacccatc attttggggt aaagcataga agtaagcata
      481 aaaggtttat aggtttcaac cattttcaaa ttcataccaa caactttttt aaatagtact
      541 gtagcggtat catattcttc ttgtgttaaa aaatctttta gtctttttcc atctttcatt
      601 ttaatatgct tcatcattgt agttgtcatt tgcggatcgt ctatatctat ttctaagaat
      661 aattgtttac acattcccat taaagatttg atggtatctg gcatttcaaa atcattagaa
      721 cataacatgt gaaaagtacc aaataagtag gagggttggt taatactatt accctcaatt
      781 ttccataata atgttttctc ttttggaatt tgagctgttg cttctgcaaa aagcatcaat
      841 gttaaaattg atagtaaagt ggttttcatt gctgtgtttt aagttagata taagttatag
      901 agtattctac aaataaacta tttagaaata aaagaaatag tgttttctaa taatttgcct
      961 gaacccctgt gtagttatat ggtgtaattt tttttagttc tttttttagc tccaaattta
     1021 tttttagaga gtctataaat aagtgaattg actgttggtt aatactactt ttacctctgg
     1081 ttaattcttt taaagcttca taagggttag ggtaattttc cttccttaat attgtttgaa
     1141 tagcttctgc aactactgcc caattattgt ctaaatcggc attaatttta ttttcattca
     1201 atagcaactt acctaaacct ttttcaatgg attttattgc aagtagggta tgagcaattg
     1261 gcacacccaa gtttctaagt actgtactat ccgttaaatc tctttgtaat ctgctaatag
     1321 gtaatttggc tgacaggtgt tctaacaaag cattagcaac tcctaaattt ccctctgcat
     1381 tttcaaaatc tatcggattt actttgtgtg gcattgcact agagcctact tctcctttct
     1441 ttgttttttg tttaaaataa tcaaggctaa tataagtcca tatatctcta cacaaatcaa
     1501 taagaatagt atttattctt tttatacaat ctaaatgtgc agctaagtta tcataatgtt
     1561 ctatttgcgt tgtaaactgc attcgttgta gcccaagaat tttatctaca aattcatttc
     1621 caaatgcaat ccattttttg tttggaaaag caacaacatg tgcattaaaa tttcctgttg
     1681 ctccaccaaa ttttgcacta aaaggtatat aagaaaattg cattatttgt ccttctattc
     1741 tttcagcaaa aaccattatt tctttaccta aaatggtagg agaagcaggt tggccatgtg
     1801 tacgtgcaag catggtaact gatttccatt ttctggccat tcttactaat aaattatgtg
     1861 tatttgcaat cattggtaaa tactcatttt ctgcacaatg tttccagctt agtggtatag
     1921 cagtattgtt aatatcctga ctggttaaac caaagtgaac ccactctttt aaattaccta
     1981 aattttcttt atccagttct tctttaataa aatattcaac agcttttaca tcatggttgg
     2041 taattgcttc tatattttta atcttgttag catcttcaat actaaagttt tctttaactt
     2101 ttaataagcg ttgtttgttg gttgaattaa tcttaaaaac acccttttct gctaaaaaca
     2161 aaaaataatc tatttcaatt gctactctat atttaattaa agcaaactcc gaaaaatagt
     2221 catctaaaaa tgcaagttgt tttctgtatc tgccatcaat aggagatata gcagtaaggt
     2281 tatttaattc catgttgatt tttatataag ttgaagtata gggatatata tagaaaaaac
     2341 attttctttg tatgcaaatt taataggatt gaataagata aaaataggag cagtaagtta
     2401 tttaaacaca aagcctttat tatatggaat taaacattct gcaataatta ataatattga
     2461 actaatagaa gaccatcctg caaatattgc tgagttgtta ttagctaata aaattgatat
     2521 agggttggtg ccaacagcta ttataccaca aattagcaat gctacaattg ttgctgatta
     2581 ctgcattgga gcaaatgcta ctgtagcaag tgtttgtttg ttttctgaaa ctactattga
     2641 taatattgaa gcagtaatct tagattacca aagcagaaca tctgtagcat tatgtaaaat
     2701 tttacttaaa gagtattgga agagagaggt gaaatttatt gaagggagag aaggctatag
     2761 aaatgaaata aaaggaacta ctgctgcaat agttattggt gatagagcct ttgaacaagc
     2821 a
//
LOCUS       DAOQHP010000300         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24469_length_2813_cov_3.682741, whole genome shotgun sequence.
ACCESSION   DAOQHP010000300 DAOQHP010000000
VERSION     DAOQHP010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24469_length_2813_cov_3.682741"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1933)
                     /locus_tag="PLY81_08915"
     CDS             complement(<1..1933)
                     /locus_tag="PLY81_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30386.1"
                     /translation="MSLKFVFTLLFFTISSALIAQKKTDTSYFMNNYRQGDKYFRENP
                     DSAIFYYKKAEEWLLKNSWFDSSIDATTMLSNMGRTYRVVGNPLSSQQTLQKALNNAR
                     KYNHNSEFRLIFLRLTFLHKEIINKNWAFNYPPVNETKTEEVYFPISKIEPLGKDSLE
                     ITVAAGKLDGIINDSQTCRINSRLIEGDTLEHKSVKTFVSVNFTKVENNFSKIHVAKS
                     FTNKILPKDFLVCYAEVPSSWDALHLKSLLLDNYNLASASDEYYNYRFFYYYGTKEIE
                     DEILDAMLTESQEIAVKVAKDTLTNKSLGSPFEGGIFSGFNLIKSLIESKIEHVKMFI
                     AYTSLYPANNMGNSPDFTNRFSSWIMTKSPLHPEFIENYFYSVSKNKSYLKTQAINLY
                     PQIKENSLITKWIGKVLLAINDERYEIVLPLANLINSIVTENKDSSNYGWYEYLMASY
                     YRKQANTILANQYLSNAKKHFTENKNFEGSKWTETTEEKWKEPINISIGIQNGHTKIF
                     ITALSPNSNYFATGSADNNIIIWNKQTGKEVKTIYYHRGTITSLHYSPDGRYLVSAAD
                     DNTMVVWNAFNYTPLISYTTDGFVNAAKFSTDSKLLYVAEDSILSIVNPFVDTTMVVK
                     KIKLHNGMINDFVFYNNNNN"
     gene            2093..>2813
                     /locus_tag="PLY81_08920"
     CDS             2093..>2813
                     /locus_tag="PLY81_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF30387.1"
                     /translation="MVQISKRGEAMPASPIRKLVPYAEAAKKKGRTVYHLNIGQPDIE
                     TPKQVRDAIHQSDFKILEYTHSAGLESYRIKLSQYYKSVGIDVNHNEIIITTGGSEAI
                     LFGFMACLDPGDEVIIPEPFYANYNGFAIAAGINVVPITSYITNGFALPPIEEFEKKI
                     TAKTKAIVICNPNNPTGYNYSENEMQELKEIILKHNLYLFADEAYREFTYEEKQHSAM
                     HLKGVENNVVLMDTISKRYSAC"
BASE COUNT          984 a          413 c          407 g         1009 t
ORIGIN      
        1 tattgttgtt gttattgtaa aaaacaaaat cattaatcat accattatgt agttttattt
       61 tttttacaac cattgtggta tctacaaatg gatttacaat actcaaaata ctgtcttcgg
      121 caacatacaa aagtttacta tctgtagaaa attttgcagc atttacaaaa ccatccgtag
      181 tataagaaat aagtggggta tagttaaagg cattccaaac aaccatggtg ttatcatctg
      241 ctgcacttac aaggtatctt ccatcggggc tataatgtaa acttgtaatt gttcctctgt
      301 gataataaat tgtttttact tctttgccag tttgtttatt ccaaatgatt atattattat
      361 ctgcactacc tgttgcaaaa tagttactat tgggtgataa agcagtaata aatatttttg
      421 tatgaccatt ttgaatacca atagaaatgt ttattggttc cttccatttt tcttcagtgg
      481 tttctgtcca tttacttcct tcaaaatttt tattctctgt aaaatgtttt tttgcattgg
      541 aaagatattg gttagcaagg atagtattgg cttgttttct ataatagcta gccatcaaat
      601 actcgtacca accataatta gagctatctt tattttcagt aactattgaa tttattaaat
      661 tagctagtgg taaaacaatt tcatacctct catcattaat agccaacaat actttaccta
      721 tccattttgt tattaaagaa ttttctttga tttgtggata taaattaatg gcttgagttt
      781 ttaaatagga tttgttttta gatacagaat aaaaataatt ttcaataaat tctggatgga
      841 gaggtgattt tgtcattatc caactagaaa atctgtttgt aaaatctggg ctattgccca
      901 tattatttgc agggtataaa cttgtataag ctataaacat ttttacatgt tctatcttac
      961 tttctattaa tgattttatt aaattaaatc ccgaaaaaat tccaccttca aaagggctac
     1021 ctaaactctt gtttgttaaa gtatcttttg caactttaac tgcaatttct tgcgattcag
     1081 ttaacatagc atccaaaatt tcatcttcta tttcttttgt tccataataa taaaaaaaac
     1141 gataattata atattcatct gaagcacttg ctaaattata attatctaac agtaaacttt
     1201 ttaaatgtaa agcatcccaa ctagaaggga cttcggcata acaaactaaa aaatctttgg
     1261 gtaaaatttt attagtaaaa ctctttgcaa catgtatttt agaaaaatta ttctctactt
     1321 tagtaaagtt tacacttaca aatgttttta ctgatttgtg ctctaaagta tctccttcaa
     1381 taagtctaga atttattcta caagtttgag agtcgtttat aataccatct aatttacctg
     1441 cagctactgt aatctctaaa gaatctttac ctaaaggttc tattttactt atagggaaat
     1501 aaacttcttc tgtttttgtt tcattaactg gtggatagtt gaaagcccaa tttttattga
     1561 taatttcttt atgtaaaaaa gtaagcctca aaaaaattaa tctaaattca gagttgtgat
     1621 tatattttct tgcattgttt aaggctttct gtaatgtttg ctgagaagat aaagggttgc
     1681 ctacaactct gtaagtacgg cccatattag ataacattgt ggttgcatca atggagctat
     1741 caaaccaaga attttttaaa agccattctt cggccttttt gtagtaaaat attgcactat
     1801 ctggattttc tctaaaatat ttatcaccct gtctataatt gttcatgaaa taggaagtgt
     1861 ctgttttttt ttgagcaatt agtgccgaag aaatagtaaa aaacagtaga gtaaatacaa
     1921 actttaaaga catttttttt attttaaagg taaatgaaat taatattata gaaataccca
     1981 acagcactta tttacattct ttaccattag gttattttta aatatctctc ttaaaatgat
     2041 aaaagaatat gattaaataa aacttgaaaa atttactact tatatttgcc acatggtaca
     2101 aataagtaaa cgaggagagg caatgcctgc ttcgccaatt agaaaactag ttccttatgc
     2161 tgaggcagca aaaaagaaag gaagaacagt ttatcattta aacatagggc aacccgatat
     2221 tgaaactcca aaacaagtaa gagatgctat acaccaatca gattttaaga tattagaata
     2281 tacacatagt gcaggtttag aaagctatag aattaaatta tctcaatatt ataaaagtgt
     2341 aggaattgat gtaaaccaca acgaaataat tattacaact ggtggaagtg aagcaatatt
     2401 atttggcttt atggcatgtt tagacccagg cgatgaagta attattccag aaccttttta
     2461 tgcaaactat aatggctttg ccattgctgc aggtataaat gtggtgccaa taacatctta
     2521 tattaccaat ggttttgcat taccaccaat agaagagttt gagaaaaaaa taactgccaa
     2581 aacaaaggct attgtaattt gtaatcctaa taatccaact ggttacaatt acagcgaaaa
     2641 tgaaatgcaa gaactaaaag aaattatctt aaaacacaac ttgtatttat ttgccgatga
     2701 agcctataga gaatttacct acgaagaaaa acaacatagt gccatgcact taaaaggtgt
     2761 agaaaataat gttgttttaa tggataccat tagtaaaagg tatagtgctt gtg
//
LOCUS       DAOQHP010000301         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24601_length_2804_cov_3.332121, whole genome shotgun sequence.
ACCESSION   DAOQHP010000301 DAOQHP010000000
VERSION     DAOQHP010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24601_length_2804_cov_3.332121"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLY81_08925"
     CDS             complement(<1..589)
                     /locus_tag="PLY81_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF30388.1"
                     /translation="MQKKNITIAGAGLVGSLLGIYLAKRGYSVSIYERRSDMRKESLS
                     AGRSINLALSDRGLLALKKVGLEDEIKKISIPMHGRYIHNADGSTAFQPYGKEGQFIN
                     SVSRATLNIKLMELAEQEGVKIYFNHKCANINWNKNEINFDTENGNSITTNFDLLFGA
                     DGAYAATRLQHQVHHSKFDYQQYYIDCGYKELTIPP"
     gene            complement(659..>2804)
                     /locus_tag="PLY81_08930"
     CDS             complement(659..>2804)
                     /locus_tag="PLY81_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30389.1"
                     /translation="YYLNFIEVGINKGNYIPLFNSANGRVFQYIAPINGVKQGNFEPA
                     IFLVTPKKQQIFTANAIYNINRKTTLITDVALSNYDVNTFSSKNKNDNIGFAAKFLLQ
                     NNNTFHNKYQLQTKIGYEWVNEKFKPIERLRTVEFARDWGLPLITSLATEHLPKVSLE
                     LKNLNNNNKIQLTTNGYLRSDKYYAIKNQVSLYQYQPKNAISIKLNSSLVNIFTPNSK
                     GYFVKINSEVSRVLKQINNYELGFNYSLERNLQSNKFTTSLEPISFSFENIGAFIKSN
                     ERKNNQWAFHFFTRKNELPFQSQLLQTDRSNNYNFSLNLLNNHNHQLKFNATYRQLFV
                     QNNFLTNLQPDKTLLSRTEYKVNEWNGFLTGFVMYELGTGQEQKRDLTYIEVPAGRGE
                     YTWNDYNLDGIPQLNEFEIALYPDLAKYIRIFTPTNQFVKASYLQFNYNLQITPKAIM
                     AKFRNEKFKKFISKFMFQSSLQTSQKVIADNKIQFNPFKKNITDTTLIHIQQQLSNTI
                     SFNRYSNIWGIDVSNFQNKSKALLTYGFESRWMNEWLVKTRINFKKVYTLELLNKFST
                     TSLITPSFNNRNYNIKSFSVEPKLTYTYLTRYRLQLSYIFVQKENNIIYGGEKAINNI
                     IQAEAKLNIFNNTSLLTKFNMQHINFNGNIGTTTSYIMLDALLPGKNYLWMVELTKRL
                     GNNLEFNINYEGRKPASSKMIHIGRAGIRALL"
BASE COUNT          923 a          404 c          384 g         1093 t
ORIGIN      
        1 ttggtggtat agttaactct ttataaccac aatctatata atattgttgg taatcaaatt
       61 tagaatggtg tacctgatgt tgcaaccttg tagcagcata agctccatcg gctccaaaaa
      121 gtaaatcaaa gtttgtagta atggagttgc cattttcggt atcaaaattt atttcatttt
      181 tgttccaatt tatattggca catttatgat taaaataaat cttcactcct tcttgttcgg
      241 ctaactccat caactttata ttaagtgtag ctctagaaac agagtttata aattgtcctt
      301 ctttaccata aggttgaaag gcagtagaac catctgcatt atgaatataa cgaccatgca
      361 ttggtattga aattttttta atctcatctt ctaacccaac ttttttcaaa gcaagcaatc
      421 ctctatcact taatgctaaa ttaatagaac gccctgctga taaagattct ttacgcatat
      481 ctgaacggcg ttcataaata gaaacagaat accctctttt tgctaaataa attcctagta
      541 aagagcctac caaccctgca ccagctatag tgatgttctt tttttgcata cgctaaatta
      601 aaatgaaaaa atataccgtt acaaatgtaa ttcttagatg ttatttaatt tatattaatt
      661 atagcaaggc tctaataccc gccctaccaa tatgtatcat ttttgaagat gctggctttc
      721 taccctcata attaatgtta aattctaaat tattgcctaa cctttttgta agctcaacca
      781 tccataaata attttttcct ggcagcaaag catctaacat aatataactt gtagttgtac
      841 caatattgcc attaaaatta atatgctgca tattaaactt tgtaagtaaa ctagtgttgt
      901 taaaaatgtt cagcttagcc tctgcttgta taatgttatt aattgctttt tcgcctccat
      961 aaataatatt gttttctttt tgaacaaata tgtaggagag ctgtaaacga taacgagtta
     1021 aataagtata agttaatttg ggctccacac taaaactttt aatattatag tttctattat
     1081 taaaagatgg tgtaattaaa gaagtagttg aaaatttatt taatagttcc aacgtatata
     1141 cttttttgaa atttattctt gttttaacta accattcatt catccatctg ctttcaaaac
     1201 cgtacgttaa taaggctttt gatttatttt gaaaattgct aacatctata ccccaaatat
     1261 tactatacct attaaaagaa atggtattac ttaattgttg ttgaatatga attaaggtag
     1321 tatctgttat atttttttta aatggattaa attgaatttt attatctgca ataacttttt
     1381 gagaggtttg taatgatgat tgaaacatga atttagaaat aaatttttta aacttttcgt
     1441 ttctaaattt agccataatt gctttgggtg taatttgtaa attgtaatta aactgtaagt
     1501 aagatgcttt tacaaattga ttagttggag taaatattct aatatacttg gctaaatccg
     1561 gatataaagc aatttcaaat tcatttaatt gcggaatacc atctaaattg taatcattcc
     1621 atgtatattc tcccctacca gctggtactt ctatataagt aagatctctt ttttgttctt
     1681 ggccagttcc taattcgtac attacaaaac ctgttaagaa accattccat tcatttacct
     1741 tatactctgt tcggctcaat aaagttttat ctggttgtag gttggttaaa aaattatttt
     1801 gaacaaataa ctgcctgtag gttgcattaa atttaagttg gtgattatgg ttgttcaata
     1861 aatttagact aaaattatag ttattacttc tatctgtttg taataactgg ctttgaaaag
     1921 gcagttcatt ttttcgtgta aaaaaatgaa aagcccattg attatttttt ctttcattac
     1981 tttttataaa tgctcctata ttttcaaaag aaaaagaaat aggctctaag ctagtagtaa
     2041 atttattgga ttgtagattt ctttctaaac tataattaaa gcccagttca taattattaa
     2101 tttgttttaa tactctggaa acttcagagt taatttttac aaaatagcct ttactatttg
     2161 gtgtaaaaat atttaccaga cttgaattta attttatgga aattgcattt ttgggttgat
     2221 attgataaag tgaaacttgg ttttttattg catagtattt gtcactcctt aaatacccat
     2281 ttgttgtaag ctgtatttta ttattattgt ttaaattttt taactcaaga gatactttag
     2341 gtaaatgttc agtagccaag ctggtaatta atggcaagcc ccaatctctt gcaaattcaa
     2401 ctgtacgaag cctttcaata ggtttgaatt tttcattaac ccattcgtat ccaattttag
     2461 tttgtagttg atatttatta tggaaggtat tattattttg aagtaaaaat ttagcagcaa
     2521 atccaatatt gtcattttta tttttagaag aaaaagtgtt tacatcataa ttgctcaagg
     2581 caacatcggt aattaaagtt gtttttctat tgatattgta tattgcatta gcagtaaaaa
     2641 tttgttgctt ttttggggtt actaaaaaaa tagctggttc aaaatttcct tgttttacac
     2701 cattaatagg ggcaatatat tgaaaaactc ttccattagc actattaaaa agaggtatat
     2761 aatttccttt attaatgcct acctcaataa agtttaaata atat
//
LOCUS       DAOQHP010000302         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24603_length_2804_cov_2.530738, whole genome shotgun sequence.
ACCESSION   DAOQHP010000302 DAOQHP010000000
VERSION     DAOQHP010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24603_length_2804_cov_2.530738"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLY81_08935"
     CDS             <1..562
                     /locus_tag="PLY81_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNF30390.1"
                     /translation="LEEEEFKKAQNCDKADINVWELINLANHHPRVKILQPGAGVGGH
                     CIAVDPWFIVSDFPTEAKLIKAGREVNDYKTEWVVEKIKNTALQFELNNKHKPVIACM
                     GLAFKPDIDDLRESPALEIAKCINDEVCTVVCVEPNVNELKGLTLVSLNQAIEQADII
                     CYLVKHSAFKGLKNTTNKIELDFCDV"
     gene            617..1912
                     /locus_tag="PLY81_08940"
     CDS             617..1912
                     /locus_tag="PLY81_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HNF30391.1"
                     /translation="MKLSTLIDSKNINWPLYFYLLILTQSIFFIKFFAFPLFFLFHFT
                     DFKIVRKYGIQYKFYIAIITYAALFCLLYLLKDFNLQYLIVVLLGLFVWLLCLINNFI
                     VNYSVHKLQLKKLYATIQFYFLLNIVLSFFQFLLLIAENKTINPFSTALGTSAGDFIK
                     GYFANSSINMIVSSFAFVFFYARKNYLLAILSLMVLILTTYMSGVLIFIVIFVLYFLL
                     TSTTSLIQKIQLLILGFIFSISFYLLSKDNIDYAVRVGSTVVEEVKPRKQVSFEQTGE
                     AILSSTSVFFFGYGMGNFSSRLAFIAGGEYVSWYPTALVRKSVEFYNNHFQLWNYEVL
                     DVPFSDGTANQPFSVFNQFLGEYGFIGTIILLYFYFYSLFKQSKKYSFIRLLLVLLFF
                     YMLLDYWFEFFSVIVVFELIVAYWLKEASEKIKHKQIVA"
     gene            1924..>2804
                     /locus_tag="PLY81_08945"
     CDS             1924..>2804
                     /locus_tag="PLY81_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF30392.1"
                     /translation="MKKEIIINTFHLFLNKGVNALVPLLLIPLLNEKFGIVGYGQLVY
                     IQAMLTVFLLIADYGFIVTGTRDISLFEENIEERNKIASAIFFIKALLISVGFIACVV
                     YFIFFYSGSQFVFLLSMAVLISFFMQSFVPSWFFQGIKKNKYISSINFITKILFFISV
                     FYFLNKGATLIAVPVLEFTSYLIAIIICIYLLYRYEKFKFKFPSLSLIKDQLKQGASI
                     FIISILNWLILAGSIFFVEKYTSKQSEEYGYYAAFSRLAYYIFAIIQPINQAIFPYFV
                     SSFSNKAANASKFVKST"
BASE COUNT          905 a          348 c          423 g         1128 t
ORIGIN      
        1 attagaagaa gaagaattta agaaagctca aaattgtgat aaagcagata taaatgtttg
       61 ggagttaatt aatttagcaa accatcatcc aagagtaaaa attttacaac caggtgcagg
      121 tgttggtggg cattgtattg ctgttgaccc ttggtttatt gtatctgatt ttcctacaga
      181 agccaaatta ataaaagctg gtagagaagt aaatgattat aaaacagaat gggttgtaga
      241 aaaaataaaa aacacagctt tacagtttga attaaataat aaacacaaac cagttatagc
      301 ttgcatggga ttggcgttta agccagatat tgatgattta agagaaagcc ctgcattaga
      361 aattgccaaa tgtattaatg atgaagtttg tactgttgta tgtgttgagc caaatgtaaa
      421 tgaactaaag ggcttaacat tagtttcact aaaccaagca atagagcaag cggatattat
      481 ttgttattta gtaaaacaca gtgcatttaa ggggttaaaa aataccacca ataaaattga
      541 gttagatttt tgtgatgttt aatttaggta tttaattatc aacaattaaa ctactgcaaa
      601 taagaaaagt aatttaatga aactttctac tttaatagat agtaaaaaca tcaattggcc
      661 gttatacttt tacttattaa tacttactca aagtattttt tttataaaat tttttgcatt
      721 tccacttttt tttctttttc attttaccga ttttaaaatt gtaagaaaat atggaataca
      781 gtataaattt tatattgcaa ttattactta tgcagctttg ttttgcttgt tatatttatt
      841 aaaagatttt aacctgcagt accttattgt agtattgtta ggcttgtttg tttggttgtt
      901 atgtttaata aataatttta ttgtaaacta tagtgtacac aagctacaac ttaaaaaatt
      961 gtatgctacc atacaatttt attttctcct taatattgtt ttatcatttt ttcaattttt
     1021 attattaata gcagaaaata aaactatcaa ccctttttct acagctttag gtaccagtgc
     1081 aggagatttt attaaaggct attttgccaa ctcttctatc aatatgatag tttcctcttt
     1141 tgcatttgtt tttttttatg ctagaaaaaa ctacttactg gctatacttt ccctaatggt
     1201 attaatactt acaacgtata tgtctggtgt gcttattttt attgtcatat ttgttctata
     1261 ttttttactt acttctacaa caagcctaat tcaaaaaatt caattattaa tattaggatt
     1321 tattttctcc atttcatttt atttactctc aaaagataat atagattatg cagtaagagt
     1381 tggaagtaca gttgtagaag aagtgaaacc tagaaaacaa gtgtcttttg agcaaacagg
     1441 cgaagccatc ttatcttcaa cttcagtttt cttttttgga tatggaatgg gaaatttttc
     1501 atcgagatta gcatttattg ctggtggaga atatgtatct tggtatccca cagcattggt
     1561 aagaaaatct gtagagtttt ataataatca ctttcagcta tggaattatg aagttttaga
     1621 tgttccattt tccgatggta ctgctaatca gccattttct gtttttaatc aatttttagg
     1681 tgagtatggg tttattggaa caatcatttt attatatttt tatttctata gtttatttaa
     1741 gcaatcgaaa aagtatagtt ttattaggct gcttttagta ttgctgtttt tttatatgct
     1801 attagattat tggtttgaat ttttctctgt tattgttgtt tttgaattaa tagttgctta
     1861 ctggctaaag gaggcttcag aaaaaattaa gcataaacaa attgttgctt aattattatt
     1921 attttgaaga aagagataat cataaatact tttcatttat ttcttaacaa aggggttaat
     1981 gctcttgttc ctttgctttt aataccatta ttaaacgaaa aatttggaat agttggctat
     2041 ggacagttag tgtatataca ggcaatgctt actgtttttt tattaattgc cgattatggc
     2101 tttattgtaa ctggcaccag agatattagt ttgtttgaag aaaatattga agaacgtaac
     2161 aaaattgctt ctgctatttt ttttataaaa gcgttattaa ttagtgtagg gtttattgcc
     2221 tgtgttgttt attttatttt tttttattct ggtagtcaat ttgtgttttt attatccatg
     2281 gcagttctta tttctttttt tatgcaaagc tttgttccat cctggttttt tcaaggcatt
     2341 aaaaaaaata agtacatttc gtctataaac tttattacca aaatattgtt ttttataagt
     2401 gtattttatt ttttaaacaa gggagcaacg ctaatagcag tacctgttct agaatttacc
     2461 tcttatttaa ttgctataat tatatgtatt tatttgttat acaggtatga aaaatttaaa
     2521 tttaaatttc cgtcattatc gctaataaaa gatcagttaa aacaaggtgc aagtattttt
     2581 atcatttcaa ttttaaattg gctaatactt gcaggcagta ttttttttgt agaaaaatat
     2641 acaagtaaac aatctgaaga gtacggctat tatgcagcat tttcaagatt ggcttattat
     2701 atttttgcca taattcaacc aattaaccag gctatttttc catattttgt ttcctccttt
     2761 tccaacaaag ctgcaaatgc cagtaagttt gtaaaatcta cttt
//
LOCUS       DAOQHP010000303         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24859_length_2786_cov_3.771146, whole genome shotgun sequence.
ACCESSION   DAOQHP010000303 DAOQHP010000000
VERSION     DAOQHP010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24859_length_2786_cov_3.771146"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLY81_08950"
     CDS             <1..393
                     /locus_tag="PLY81_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNF30393.1"
                     /translation="TTGGNALKFYASVRLDIRRAAQIKDGDSAIGNHVKVKVVKNKVA
                     PPFRSAEFDIIFGEGISKIGEIIDMGVELGIVNKSGSWFSYNSEKLGQGRDAVKQLLL
                     DNAELANEIEAKIRAKLAELQSTATVEA"
     gene            530..1135
                     /locus_tag="PLY81_08955"
     CDS             530..1135
                     /locus_tag="PLY81_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30394.1"
                     /translation="MKKLFILSALFAVISFTATAQVKIKLEPAAAFGKKAISALKTQG
                     TQQTMKAVAEGEECGAFVATKKDDKGANYTEAKEFLIVSNDGVTGFAFTMYATTYEAK
                     TLYVVRGMPLETGVCVDKSTKVTFTFEDGTQRTFTNFEKDNCKGIVTLYMHELVKNMD
                     AYEDLKTKKVRSIRLEGIEKTVVKSLSEGNQQQLQGTIKCL"
     gene            complement(1201..2478)
                     /gene="tyrS"
                     /locus_tag="PLY81_08960"
     CDS             complement(1201..2478)
                     /gene="tyrS"
                     /locus_tag="PLY81_08960"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNF30395.1"
                     /translation="MNLIEELRWRGMIADIMPGTEEQLVKEMTTGYVGFDPTAESLHI
                     GSLVPIILLVHMQKSGHKPMALIGGATGMIGDPTGKSDERNLLSEETLQKNIEGIQSQ
                     LKQFISFDDEKPNAAILVNNYDWFKNISFIDFLRDTGKHITINYMMAKDSVKKRIEGE
                     NGISYTEFAYQLMQGYDFYWLYKNKNCKLQFGGSDQWGNMTTGSELIRRMCGGEAFVF
                     TCPLITKADGGKFGKTEGGNVWLSADKTTPYQFYQFWLNTSDADAEKWIKIFTFLTKE
                     EINSLIIQHQQNASDRVLQKTLAKEVTIFVHGENEYEKAVETTQKLFANANAPIESLS
                     VDDLENMEGVQKIKLDLHNLKQGMDIISFLADSSILASKGEARKMLQNGGISINRQKI
                     SDVQFIVQNSLLLHQKYLLVQKGKKNYYLVSFE"
BASE COUNT          937 a          426 c          418 g         1005 t
ORIGIN      
        1 acaactggtg gtaatgcatt aaaattttat gcctctgtac gtttagatat tagaagagca
       61 gcacaaataa aagatggcga ctctgcaata ggcaatcatg taaaagtaaa agttgtaaaa
      121 aataaagtag cacctccttt tagaagtgca gagtttgata ttatttttgg agaaggtatt
      181 agtaaaatag gagaaataat agatatgggt gtggaattag gtattgtaaa taaaagtggt
      241 agttggttta gctataatag tgaaaagtta gggcagggta gagatgctgt aaagcaactt
      301 ttattagata atgcagaatt ggcaaacgaa attgaagcca aaattagagc caaattagcc
      361 gagctgcaat ctactgcaac tgtagaagca taatagaaaa ctttatatct aaaaaattaa
      421 atgttaaata acgttaataa aatataaata ccttattttg ggtattgtat ttgattaagt
      481 acccaattat ttttacatta aaattattaa aacacaaaat ttaaaaaaca tgaaaaaatt
      541 atttatcctt tcagcattat ttgcagtaat atcttttact gcaactgcac aagttaaaat
      601 taaattagaa cctgctgcag catttggtaa aaaagcaata tctgcattaa aaactcaagg
      661 cacacaacaa accatgaagg ctgttgcaga aggtgaagag tgtggtgctt ttgttgctac
      721 caaaaaagac gataaaggag ccaattacac ggaagctaaa gagtttttaa ttgtatctaa
      781 cgatggagta actggttttg catttacaat gtacgcaact acttacgaag ctaaaacact
      841 ttatgttgta agaggtatgc ctttagaaac tggagtttgt gttgataaat ctactaaagt
      901 tacttttact tttgaagatg gtacacaaag aacttttact aattttgaga aagataattg
      961 taaaggtatt gttactttat atatgcatga acttgtaaaa aatatggatg cttacgaaga
     1021 tttaaaaact aaaaaagtaa gaagtattag attagaaggt attgaaaaaa ctgttgttaa
     1081 atctttaagc gaaggtaatc aacaacaatt acaaggtact attaaatgtt tataattgta
     1141 agcaacttta tttaaaaatt aagggctgca ttaaaaatgc agcccttgtt ttttagcaac
     1201 ttattcaaac gaaactagat agtaattttt tttgcctttt tgcaccaata aatatttttg
     1261 atgtaatagt aaactgtttt gtacaataaa ttgcacatcg cttatttttt gcctgttaat
     1321 gctaatacca ccattttgca acatttttct agcttctcct ttactagcta aaatagaaga
     1381 atctgctaaa aaagaaatta tatccattcc ttgttttaag ttatgtaagt caagttttat
     1441 tttttgcaca ccttccatat tctctaaatc atctacactt aaactttcta taggagcatt
     1501 ggcattagca aataattttt gagttgtttc aacagctttt tcatattcat tttctccgtg
     1561 aacaaaaatg gttacttctt tagccaatgt tttttgtaaa actctatccg atgcattttg
     1621 ttgatgctgt ataattaaac tattaatttc ttctttagtt aaaaatgtaa aaattttaat
     1681 ccatttttct gcatcggcat cgcttgtgtt taaccaaaat tgataaaact gatagggtgt
     1741 agttttatcg gcacttaacc aaacatttcc tccttctgtt tttccaaatt ttcctccatc
     1801 ggctttggtt attagcggac atgtaaatac aaaagcttct ccaccacaca ttcttctaat
     1861 taactcagag ccagttgtca tgtttcccca ctggtcactt ccaccaaatt gtagtttaca
     1921 gttcttattt ttatacaacc agtaaaaatc gtaaccctgc attaattgat aggcaaattc
     1981 ggtataacta attccgtttt ctccttcaat tcttttcttt acactatctt tagccatcat
     2041 ataatttatg gttatatgct tgcccgtgtc tcttaaaaaa tcaataaatg aaatattttt
     2101 aaaccaatca taattattta ccagtattgc tgcatttggt ttttcatcat caaaagatat
     2161 aaattgttta agttgtgatt gaataccttc tatatttttt tgaagtgttt cttcgctcaa
     2221 taaatttctt tcgtcacttt ttcctgttgg gtctcctatc atacctgttg caccaccaat
     2281 taaagccatt ggcttgtgcc cagatttttg catgtgtact aaaagtatta ttggtacaag
     2341 gctaccaata tgcaggcttt ctgcagtagg atcaaaacct acataacctg tagtcatttc
     2401 tttaactaat tgttcttcag tgcctggcat tatatcagca atcattccac gccaacgtaa
     2461 ttcctcaatt aaattcatac aaaatactat ttgggcaaat gtaaaagatt agccttattg
     2521 aataatgtga atatcccctt taaaagataa ttttttaaac attttttcgt ttttaacttg
     2581 ttattactgc atttaagaca atttttagcg aaatttgtaa gcagccttta aacaacctta
     2641 gctatctttt gttggatgag tgggaacgtt ttttgttaat accgaacttt tatgaattaa
     2701 tttattatga aacatttgct actaactttt tttattgttt gttttgggaa ttcaattatt
     2761 gcacaacctt tacataatga gtggat
//
LOCUS       DAOQHP010000304         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_24915_length_2782_cov_3.747708, whole genome shotgun sequence.
ACCESSION   DAOQHP010000304 DAOQHP010000000
VERSION     DAOQHP010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24915_length_2782_cov_3.747708"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLY81_08965"
     CDS             <1..362
                     /locus_tag="PLY81_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF30396.1"
                     /translation="EFYNGAIGTINYTVNSYQKNMEGSLTIFAEKGTVKIGGQYLNEL
                     EYQAIENFEIKDLPTGNTANNYGTYVGSMSNHDKVYENLIDVLQNGKSIATNSFEGLK
                     TVEIISKIYQSAKELNK"
     gene            390..1496
                     /locus_tag="PLY81_08970"
     CDS             390..1496
                     /locus_tag="PLY81_08970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF30397.1"
                     /translation="MNIPFVDLKQQYQSIKNEIDTAIENVLTETAFIGGKYVNKFEKD
                     FETLYGVNHCISCANGTDSLYIIMKMLGIGAGDEVITVANSWISSSETISQTGAKPVF
                     IDIEEEYLSINEKLIEQKITSKTKALIMVHLHGQMCDIETIAQLCKKYQVYLIEDCAQ
                     SHLSEYNNIRAGLRGIAASFSFYPGKNLGAYGDAGCIITNDSALAEKCKMYARHGALI
                     KHQHKIEGINSRLDGLQAAVLSAKLPFLIEWTNKRIAAAKHYDEALKNINEIVTPKVR
                     ANSKHTFHLYVIRAKQRNELANYLKEKGIETAIHYPTPLPFLEAYNYLNYTELDFPVA
                     NKFKNEILSLPIYPEITIEQINYIALTIKSFYSK"
     gene            1493..2485
                     /locus_tag="PLY81_08975"
     CDS             1493..2485
                     /locus_tag="PLY81_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF30398.1"
                     /translation="MIISIISPVFNEEKYIHQTIKSFLEQEHTSFDVEILLCDGMSTD
                     KTRDIIKSYAEQYANIRLIDNPKRKTPFAFNIGIKEARGEYVAILGAHSAYDKNYLQA
                     CYNELIATNSIACSGRVITQSAFDTLEAKLCEWAMLSQFGVSGNSFRLMKEGYTHSVN
                     FPVVKKEALLSLGGYNEMLERNQDNDMNQRLIDAGYQLYCTWKTKCFYRPPATLNKLF
                     KYGYRGGFWNAKSFFAHKKSMRIHHFIPFFFVVALLTTFTLGVVEFVLGKSSYFFSLF
                     SLIVFFHLLAGLFFSAISLKYESDSRKIFLPFVFFLFHFSYGWGTVKGFVDYKK"
     gene            complement(2512..>2782)
                     /locus_tag="PLY81_08980"
     CDS             complement(2512..>2782)
                     /locus_tag="PLY81_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNF30399.1"
                     /translation="FVNKTIRESKIREQTHGIVVGVETNGERIVNPESVYTIKTGDIL
                     WIVGDKLRIAAIKKAEEELATILNNNAKEGEDFSNYSSEESYTNT"
BASE COUNT          980 a          370 c          459 g          973 t
ORIGIN      
        1 tagagtttta taatggtgca attggaacca taaattatac tgtaaatagc tatcaaaaaa
       61 atatggaagg ttccttaacc atttttgcag aaaaaggaac agttaaaatt ggcggccaat
      121 atttgaatga actagaatac caagcaattg aaaattttga aataaaagat ttacctacag
      181 gcaatactgc taataactat ggtacttatg taggtagtat gagtaatcat gataaagtgt
      241 atgaaaattt aattgatgtg ttacaaaatg gtaaatcaat tgctaccaac tcatttgaag
      301 gtttaaaaac ggtagaaatt atttctaaaa tataccaatc tgccaaagaa cttaataaat
      361 aatattgaag caaattaatt ttaataatta tgaatattcc ttttgttgat cttaaacaac
      421 aataccaatc aataaaaaat gaaattgata ccgcaattga aaatgtatta actgaaactg
      481 catttatagg gggtaaatat gtaaataagt ttgaaaaaga ttttgaaaca ttgtatggtg
      541 taaaccattg cattagttgt gcaaatggta cagactcatt atacataatt atgaagatgc
      601 ttggaatagg tgctggtgat gaagtaatta ctgttgccaa tagttggatt agtagcagcg
      661 aaacaatttc tcaaactggt gcaaagcctg tatttattga tattgaggaa gaatatttat
      721 ctatcaatga aaaattaatt gaacaaaaaa taacttctaa aaccaaagca ttaattatgg
      781 tgcatttgca tgggcaaatg tgtgatattg aaactatagc tcaactatgt aagaaatatc
      841 aggtgtattt aattgaagat tgtgcccaat ctcatttaag tgagtataat aatataagag
      901 ctggtttaag aggtattgct gcatcgttta gtttttatcc tggtaaaaat ttaggggctt
      961 atggagatgc aggttgtatt ataactaatg attcggcctt ggcagaaaaa tgtaaaatgt
     1021 acgcaaggca tggtgcttta ataaaacacc aacataaaat agaaggtatc aactctaggt
     1081 tagatggctt acaagctgca gtattatctg ctaaactgcc ttttttaata gagtggacga
     1141 ataaaagaat tgctgctgca aaacattatg atgaggcttt aaaaaatata aatgaaattg
     1201 ttactccaaa agttagagct aactcaaaac atacttttca tttgtatgtt ataagagcaa
     1261 agcaaagaaa cgaattggcc aattatttaa aggaaaaagg gattgaaact gctattcatt
     1321 atccaacacc attgccattt ttagaggctt acaattattt aaattatacc gagctggatt
     1381 ttccggttgc taataaattt aaaaacgaaa ttttatcatt acccatctat ccagaaataa
     1441 caatagaaca aataaattat attgcattaa ccataaaatc tttttatagt aaatgataat
     1501 atcaattatc agcccggttt ttaatgaaga aaaatatatc catcaaacaa taaaaagttt
     1561 tttggaacag gagcatactt cttttgatgt tgaaatattg ttgtgtgatg gaatgagtac
     1621 agataaaacc agagatatca ttaaaagcta tgcagaacaa tatgccaata ttaggttaat
     1681 agataatcca aaacgtaaaa caccttttgc atttaatata ggtataaaag aagctcgtgg
     1741 agagtatgtt gccattttag gggcacatag tgcatacgat aaaaattatt tacaggcttg
     1801 ttataatgag cttattgcaa ccaactccat agcatgttcg ggtagggtaa ttactcaatc
     1861 tgcatttgat actttagaag ctaaattatg tgagtgggca atgcttagcc agtttggggt
     1921 ttcgggtaat tcttttagat taatgaaaga aggctatacc cattctgtaa attttcctgt
     1981 agtaaaaaaa gaagctttac taagcttagg tggttataac gaaatgttag aaagaaatca
     2041 ggataatgat atgaaccagc gtttaataga tgcaggctat caattatact gtacctggaa
     2101 aacaaaatgc ttttacaggc ctcctgctac tttaaacaaa ctatttaagt atggttatag
     2161 aggtggtttt tggaatgcaa aaagtttttt tgctcacaaa aaaagcatgc gtattcatca
     2221 ttttattcca ttcttttttg ttgtagcttt attaactact tttactttag gtgtagtaga
     2281 gtttgtacta ggtaagtcaa gttatttttt tagtttgttt tcattaattg tattctttca
     2341 ccttcttgcg gggcttttct tttctgctat ttcactaaaa tatgaaagtg atagtagaaa
     2401 aatatttttg ccctttgtgt tttttttatt ccattttagt tatggatggg gaactgtaaa
     2461 aggttttgtt gattataaaa aataacaatt tttaatttcc aattattttt tttatgtatt
     2521 agtataactt tcttccgatg aatagttgct aaagtcttca ccctcttttg cattattatt
     2581 taaaatagta gctaactctt cctctgcttt tttaatagct gctattctaa gtttatctcc
     2641 tacaatccat agtatatcac cagtttttat tgtatataca gattctggat taactattct
     2701 ttctccattt gtttcaactc ctacaactat tccatgtgtt tgttctctta ttttcgattc
     2761 tctaattgtt ttatttacaa at
//
LOCUS       DAOQHP010000305         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_25147_length_2765_cov_3.078967, whole genome shotgun sequence.
ACCESSION   DAOQHP010000305 DAOQHP010000000
VERSION     DAOQHP010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25147_length_2765_cov_3.078967"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLY81_08985"
     CDS             complement(<1..605)
                     /locus_tag="PLY81_08985"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF30400.1"
                     /translation="MQQSIFNYKGHNIAYTIYGSGKPLVLLHGFGEDSTIFNNQVNYL
                     KNNFQVIVPDLPGSGNSSLLKLRSVIEINKIMLHEAAITDYANCIAALLNFLAINECI
                     LLGHSMGGYITLAFAEKFPNYLKAFGLLHSTAFEDNEIKKENRMQSIAMIEEYGAFSF
                     LKNVIPNYFGKKFSMQQAPQINKLIDKSAKFTNEALQQYYFA"
     gene            complement(605..2179)
                     /gene="lnt"
                     /locus_tag="PLY81_08990"
     CDS             complement(605..2179)
                     /gene="lnt"
                     /locus_tag="PLY81_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNF30401.1"
                     /translation="MKTFKYLLLSTVAALMMYIAWPPQPITIAVFFAFIPLLFIADTV
                     SNKKLFFAFVFIALLLWNVFTTWWIWNSTSVGSIFAIVANSFVMYLPWWGYYHTKNKF
                     NKQISYLALICFWMLFEYIHLNWELSWPWLSLGNVFANSPYWIQWYEYTGVGGGTLWI
                     LFCNILIFETTKQWSTISAKVKFGYLLAVIFAISFPIIISYQIANNREKLIAKANENV
                     VIIQPNIDPYLKFESGNINSQIEKLVSLTASSIDSNTVLVLWPETALSSAIPEDAISN
                     ATVYKPIFDFLKNHPKITLVSGIETYKIMSSETKYSQKTTQDVYYESYNAAIAINSNK
                     VSNIYIKSKLVPGVETLPSFLKILSPVFERFGGTTGGYAKDETSKVFQINNQLNVAPV
                     ICYESIYGEYLSTYIKKGANLITILTNDGWWGNTAGHKQHLAYAKLRAIETRCWIVRS
                     ANTGISAVINDYGLVLNTLDWNKEGAIKQTVQINNAKSFYVQHGDYFYFIFSIIAFIL
                     IGYFLFILLRKNKKTQ"
     gene            2283..2699
                     /locus_tag="PLY81_08995"
     CDS             2283..2699
                     /locus_tag="PLY81_08995"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNF30402.1"
                     /translation="MKKILIFVPGFIWLIISLWLFTLPGTAIPKFDLFTKLQGDKLIH
                     AIIFIVLCISFLLPLHNIQPYCANNKFWFLFIILVFGMYGLAIEFVQEKFIKFRSFDV
                     GDIVADWVGCFIGFKLFIRYWIKKIGLSRNRDRNQN"
BASE COUNT          966 a          428 c          372 g          999 t
ORIGIN      
        1 gcaaaataat attgttgcaa agcctcattt gtaaacttag cagatttatc aattagctta
       61 ttaatttgag gtgcttgttg catactaaat tttttaccaa aataatttgg aataacattt
      121 tttaaaaaag agaatgcccc atattcttct atcattgcaa tactctgcat tctattttct
      181 tttttaattt cattatcttc aaaagcagtg gagtgcaata gcccaaatgc ttttaaatag
      241 tttggaaatt tttcggcaaa tgcaagtgtt atgtaaccac ccatgctatg ccccaataaa
      301 atacattcat taatggctaa aaaatttaat aatgctgcta tacaatttgc ataatctgtt
      361 attgcagctt catgaagcat aattttatta atctcaatta cacttcttaa tttaagtagg
      421 ctactattgc cagatccagg taaatccggt acaattactt gaaaattatt tttaagataa
      481 ttaacttgat tattaaaaat tgtggaatct tcaccaaaac catgtaaaag caccaagggc
      541 tttccagaac cataaattgt ataagcaata ttatgcccct tataattaaa aatactctgt
      601 tgcattattg tgttttttta ttttttctta ataaaataaa cagaaaataa ccaattaata
      661 tgaatgctat aattgaaaaa ataaagtaaa aataatctcc atgttgtaca taaaaagatt
      721 tagcattatt aatttgtaca gtttgtttta ttgcaccttc cttattccaa tctaaagtgt
      781 ttaatactaa accatagtca ttaattacag cactaatgcc agtattggca cttcttacaa
      841 tccaacatct agtttcaata gctcttaatt tggcataagc caaatgctgc ttatggcctg
      901 cagtattgcc ccaccaacca tcatttgtaa ggatggtaat taagtttgca ccttttttta
      961 tataggtgct taaatattct ccataaatac tttcataaca aataactggt gctacattaa
     1021 gttgattatt tatttgaaaa actttagagg tttcgtcttt tgcatagcca cctgttgttc
     1081 caccaaatcg ttcaaaaacc ggagaaagaa tttttaaaaa acttggtaaa gtttcaacac
     1141 ctggtactaa tttactttta atataaatat tcgaaacttt attgctatta atggctattg
     1201 cagcattata actttcgtaa taaacatctt gagtagtttt ttgagaatac tttgtttcgc
     1261 tactcattat tttataagtt tcaattcctg aaactaaagt tatttttgga tggttcttta
     1321 agaagtcaaa aatgggttta tacacagttg catttgaaat tgcatcttct ggaattgcag
     1381 aagataaagc cgtctctggc cataatacta aaactgtgtt gctatcaata gaacttgcag
     1441 ttaaggatac taatttttca atttggctat taatattacc actctcaaac ttcaggtaag
     1501 ggtctatatt aggttgaatg attactacat tttcattagc ctttgcaatt aatttttctc
     1561 tgttattggc tatttgataa gaaattatta ttggaaaact aatggcaaaa attactgcca
     1621 ataagtaacc aaattttact tttgcagaaa ttgtactcca ctgcttagta gtttcaaaaa
     1681 ttaaaatatt acaaaataaa atccataaag ttcctcctcc aacacctgta tattcatacc
     1741 attgaatcca ataggggcta ttggcaaaaa catttcctaa acttaaccaa ggccaactta
     1801 actcccaatt taaatggata tattcgaata acatccaaaa acaaattaat gccaggtaac
     1861 taatttgttt gttgaattta tttttggtat gataataacc ccaccagggc aagtacataa
     1921 caaaactatt agcaacaata gcaaaaatgc tacctacact tgtactattc catatccacc
     1981 atgttgtaaa aacattccat aatagaagag caataaatac aaatgcaaaa aatagttttt
     2041 tattactaac cgtatctgca ataaaaagta aagggataaa agcaaaaaaa acagcaattg
     2101 taataggctg tggtggccaa gcaatataca tcattaaggc tgccacagtg cttaataata
     2161 aatatttaaa agttttcaat aaagtatttt taggctgcga aagttcggaa aaaataaaca
     2221 attctttcaa ttaaattaat gcacaatgtg taatgattgt attttagctt caaaatatgt
     2281 ttttgaaaaa aattttaatt tttgtacccg gttttatttg gcttataatt agcttatggc
     2341 tttttacctt gcctggtact gctattccta aatttgattt atttacaaag ctgcaaggag
     2401 acaaactaat acatgccatt atttttatag ttttatgtat atcttttcta cttccattac
     2461 ataatattca accttattgt gccaataata aattttggtt tttattcatt atccttgttt
     2521 ttggtatgta tggtttggca atagaatttg ttcaagaaaa atttattaaa tttcgttctt
     2581 ttgatgtagg agatattgtg gcagattggg ttggttgctt tataggattt aaacttttta
     2641 ttagatattg gataaaaaaa ataggtctca gtagaaaccg agaccgcaac caaaactaac
     2701 tgcttatgag aaaaatttta aatctttatt ttattgtttg taattagtaa cgcaattaag
     2761 atgcc
//
LOCUS       DAOQHP010000306         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_25280_length_2757_cov_2.989637, whole genome shotgun sequence.
ACCESSION   DAOQHP010000306 DAOQHP010000000
VERSION     DAOQHP010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25280_length_2757_cov_2.989637"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLY81_09000"
     CDS             <1..362
                     /locus_tag="PLY81_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF30403.1"
                     /translation="YKFNNVAGYDIYADYFMQSGNKQFLGGILYGFDLVELYDSDEKI
                     SLYSGGFYRWGDALIPVIKLNYYKLAVGLSYDINVSKLTTASQARGGFEISASYRTQL
                     NIRNNGAEKMRCVKFTL"
     gene            625..1887
                     /locus_tag="PLY81_09005"
     CDS             625..1887
                     /locus_tag="PLY81_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF30404.1"
                     /translation="MTIYRSTTKTILFSALLLLSVAFSNKAVAQDGKALFNANCASCH
                     KLDKDLTGPALMGVEERWGGSREKLIHWVKNSAAFLKTGDKHANELFNKWNKIPMPGY
                     DGILSDADINAIFDYIAKGAAPAPTDNKAAEVKPVEKSNNNLLFGILTLILAIVAFVL
                     INVNSNLKRLANEEQGLASSLPIPFWKNKTYITIIAVALFVTGGYLTTKGAIGLGRNT
                     EYQPVQPIFYSHKVHAGVNQINCLYCHGGAEGGKHANIPSVNVCMNCHKAIHEYTGPA
                     LVDGNGKEVNGTEELQKLFKYAGFDPKNANNWNPNDAKPIEWVQIHSLPDHVYFNHSQ
                     HVVAGKVQCQTCHGEIDKMDEVKQVNDLSMGWCINCHRETKVQFKDNGFYSIYEKYHN
                     ELKSGKLDSTKGITVEKIGGTECQKCHY"
     gene            1976..>2757
                     /locus_tag="PLY81_09010"
     CDS             1976..>2757
                     /locus_tag="PLY81_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNF30405.1"
                     /translation="MEKKYWQSFGELNNSEAFQKSSKDEFKEDLPFDEDKGLLEAKTP
                     RRDFLKYLGFSTAAAAVAASCEVPVKKAIPFANRPEEIIPGVANYYATTYVQDGDAVS
                     VVAKVRDGRPIKIEGNELCPITSGGTSARVQASVLDLYDGARLRFPANYTSSVKGKEL
                     TFDAADKAINEALATAGNIVLLTSTINSPSSLEVINQFLAKYPGSKQVTYDAVSYSGI
                     LLANEACYNKRAIPTYRFDAAKVIVSLGADFLASWISPIEFA"
BASE COUNT          973 a          403 c          514 g          867 t
ORIGIN      
        1 aatacaaatt caataatgta gccggatatg atatttatgc agattatttt atgcaatctg
       61 gcaataaaca atttttaggt ggaatattat atggatttga tttggtagaa ctctacgatt
      121 ctgatgaaaa gatatctctt tattctggag gattttatcg ttggggcgat gctttaattc
      181 ctgttataaa actaaattat tataaattgg cagtaggttt aagttacgat ataaatgtat
      241 ctaaattaac aacagcttct caggcacgtg gagggtttga aatttctgca agctatagaa
      301 cacaattaaa cattagaaat aatggtgcag aaaaaatgcg atgtgtaaaa tttactttgt
      361 aatacttctt ttgtttgtaa aaacatttta tccattaaat actgaaacag taaaataatg
      421 gatgtttttt taacatcttt tttttacgaa aaaaggtaaa aaaatatgac aatttgaagg
      481 ccgaaatttc aatattcgct gaaagtcaag ctacataagg ataaattttt ttttttaaca
      541 ttgtgttttt gtcagattag tgtcatattt ttgcacctat tcaagcattt tttatctcat
      601 attttagaat catagcttgt gaatatgacc atctacagaa gtacaaccaa aacaatctta
      661 tttagtgcac ttttattatt aagtgttgct tttagtaata aagctgttgc ccaagatggc
      721 aaagccttat ttaatgccaa ctgtgccagt tgtcataagt tagataaaga ccttaccggt
      781 ccagcattaa tgggtgtaga agaacgctgg ggtggtagtc gtgaaaaatt aatccattgg
      841 gttaaaaatt ctgcagcctt tttaaaaaca ggcgataagc atgcaaatga actttttaac
      901 aaatggaata aaataccaat gcccggttac gatggtattt taagtgatgc agatataaat
      961 gccatttttg attatatagc aaaaggtgca gcacctgcac caacagataa caaagctgct
     1021 gaagtaaaac ctgttgaaaa aagtaacaat aaccttcttt ttggtattct aactctaatt
     1081 cttgcaatag ttgcatttgt tttaataaat gtaaacagca atttaaaaag acttgctaac
     1141 gaagaacaag gtttagcttc tagcttacct attcctttct ggaaaaataa aacttatatt
     1201 acaataattg ctgtagcctt atttgtaact ggtggctatt taacaacaaa gggtgcaatt
     1261 ggtttaggta gaaatacaga gtaccaacca gtgcaaccta ttttctactc tcacaaagta
     1321 catgctggtg taaatcaaat aaactgtttg tattgtcatg gtggtgccga gggaggtaaa
     1381 catgcaaata ttccttctgt aaatgtttgt atgaattgcc ataaagctat tcatgaatat
     1441 actgggcctg cattggtgga tggtaacggc aaagaagtaa acggaacaga agagctacaa
     1501 aaattattca aatatgcagg atttgatcct aaaaatgcta acaactggaa ccccaatgat
     1561 gcaaaaccaa tagagtgggt tcaaatacat agtttaccag atcatgttta ctttaatcac
     1621 agccaacacg tagttgcagg caaagtgcaa tgccaaacct gccatggaga aattgacaaa
     1681 atggatgaag taaaacaagt gaatgattta agtatgggct ggtgtataaa ctgccataga
     1741 gaaactaaag tgcagtttaa agacaatggt ttttatagta tttacgaaaa atatcataat
     1801 gaactaaaga gtggtaagtt agatagtaca aaaggtataa cagttgaaaa aattggcggt
     1861 accgagtgtc aaaaatgtca ctattaataa caaacattaa ttttttagtt aagtgattgt
     1921 agagtaataa tatttttata aacattaaac tgttctcaaa agaagataag ggcttatgga
     1981 aaaaaagtat tggcaaagtt ttggcgaact gaataactct gaggcttttc aaaaatcatc
     2041 aaaagatgaa tttaaagaag accttccatt cgatgaagat aaaggtttgc tagaagcaaa
     2101 aacaccacgc cgtgatttct taaaatattt aggatttagt acagcagcag ctgctgttgc
     2161 tgcaagttgc gaagtgcctg taaaaaaggc aattcctttt gcaaatcgtc cagaagaaat
     2221 tataccaggt gttgcaaatt attatgcaac aacttatgtt caagatggcg atgcagtaag
     2281 cgttgtagct aaagttagag atggccgtcc tattaaaatt gaaggaaatg aattgtgtcc
     2341 tattaccagt ggtggtacaa gtgccagagt acaagccagc gttttagatt tgtacgatgg
     2401 tgcaagattg cgttttcctg caaattatac cagtagcgta aaaggtaaag aactaacttt
     2461 tgatgccgca gataaagcaa ttaatgaagc attggctaca gctggaaata ttgttttatt
     2521 aacaagcaca attaactctc cttcttcctt agaagtaatt aaccaatttt tagcaaaata
     2581 ccctggcagt aaacaagtaa cgtatgatgc tgtttcttat tctggtattt tattagctaa
     2641 tgaggcttgt tataataaaa gagctattcc aacctatagg tttgatgcag caaaagtaat
     2701 tgtaagctta ggtgccgatt ttttagcctc ttggattagc cctatagagt ttgcaaa
//
LOCUS       DAOQHP010000307         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_25365_length_2751_cov_4.170994, whole genome shotgun sequence.
ACCESSION   DAOQHP010000307 DAOQHP010000000
VERSION     DAOQHP010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25365_length_2751_cov_4.170994"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLY81_09015"
     CDS             complement(<1..305)
                     /locus_tag="PLY81_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30406.1"
                     /translation="MNFRILIAVVFMLSCNFANAQKMHSDSTYRKKHSVKTFTQKPAQ
                     VVFAELGGNSLIWTINYDRRFSKRLDGWGFKIGAGYFPLGGSNLFVAPVGINILTGA"
     gene            complement(422..>2751)
                     /locus_tag="PLY81_09020"
     CDS             complement(422..>2751)
                     /locus_tag="PLY81_09020"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNF30407.1"
                     /translation="EWKEKYGGIILQQLKKLGCSLDWNRTAFTMDDDYYKSVINTFIQ
                     LYKDGIIYRGLRMVNWDPASKTALSDEEVIFKETDSKLYFVKYAMASDANQFIMVATT
                     RPETILGDVAICVNPTDERYKNLIGKEVIVPIINRKIPIIADEYVDVTFGTGALKITP
                     AHDKNDNELGRKYQLKAINTLDEDGKFTIDELLEDNPSEVVKNYHGIDRFELRKIIAK
                     DISELGQLEKVEEYKSQVGTSERTGAIIESRLSMQWFMNMKMFMEKNPEALNAVLNDT
                     INFHPSKLKNTYRHWLINVKDWCISRQLWWGQRIPAWYDDEGNFVVAATKEEAIIQFK
                     TQFPSSNLQTITQEEDVLDTWFSSWLWPIEVFKGISQPNNEEINYYYPTTTLVTGQDI
                     IFFWVARMIMAGYEFKQQLPFKDVYFTGMVRDKQGRKMSKQLGNSPDLLALIDTYGAD
                     AVRFGILIASPAGNDLLFDESSLEQGRNFNNKLWNALKLIKNLEARVQKSSIQTNHSA
                     NTNFAVIWFENRLNEVKQEVENLLKQFKLSEALKTIYSLIWDDYCSWYLEWVKPGFEQ
                     PMDESIYQKSVQFFDSLCMLLHPFMPFITEEIYHLLQDRNDDITVKQQSTIQSIDSSV
                     LVQAELLKKVITALREARAKNQIKQKDSIKLYIQTNSLESYSSIEIILQKQINAEFIK
                     YVTNPINNTIVVAIENEKFFIETEKEINNHALKAELEKDLAYQKSFLESVIKKLANEK
                     FVQNAKPSVIEMEQKKKADCEARIKTIEESLATLL"
BASE COUNT          843 a          499 c          342 g         1067 t
ORIGIN      
        1 ttagctcctg ttaaaatatt tatacctaca ggtgcaacaa acaaattact gccacctaaa
       61 ggaaaatatc cagcaccaat tttaaaaccc caaccatcta atcttttact aaatcttctg
      121 tcgtaattta tcgtccaaat taaactatta ccaccaagct cagcaaacac aacttgtgca
      181 ggcttttgtg taaatgtttt tacgctatgc ttttttctgt aggtagaatc ggagtgcatc
      241 ttttgagcat ttgcaaaatt gcaactaagc ataaacacaa cagcaataag tattctaaaa
      301 ttcatttgtt aacatttaaa tattttgttt cttaagaagt ataatacaaa tattttacag
      361 ccacctgcag caatactatt gaataaaatt actttattaa acccatttga actaacacat
      421 attataataa agttgctaaa ctttcttcta ttgttttaat tcttgcttca caatcggctt
      481 tcttcttttg ctccatttca attacagaag gttttgcatt ttgcacaaat ttctcgttag
      541 ctaatttctt gataacgctt tctaaaaaac ttttttgata tgctaaatct ttttctagtt
      601 cagcttttaa tgcatgatta ttaatttctt tttcggtctc aataaaaaac ttttcgtttt
      661 caatagcaac tacaatagta ttatttattg ggttggtaac atacttaata aactctgcat
      721 taatctgttt ttgcaaaatt atttcaatag atgaataact ttctaaagag ttggtttgta
      781 tatatagttt aatgctatcc ttttgtttaa tttggttttt tgcccttgct tctcttaatg
      841 ctgttattac tttttttagt aattctgctt gtactaaaac ggaactatct atactttgaa
      901 ttgtggattg ttgtttaaca gtaatatcat catttctatc ttgtaataaa tgatatattt
      961 cttctgttat aaaaggcata aaaggatgta ataacataca caaactatca aaaaattgaa
     1021 cagatttttg atagatactt tcatccattg gttgctcaaa acctggttta acccattcaa
     1081 gataccaact gcaatagtca tcccaaatta aagagtaaat tgtttttaag gcttcactta
     1141 atttaaactg ttttaataaa ttttcaactt cttgcttaac ttcatttagc ctgttttcaa
     1201 accaaattac agcaaaatta gtgtttgctg aatggttagt ttgtatgcta ctcttttgaa
     1261 ctcttgcctc taaattctta atcaatttta aagcattcca tagtttatta ttaaagtttc
     1321 tgccttgttc taaactactt tcatcaaata ataaatcgtt accagcaggc gaagcaatta
     1381 agattccaaa acgtactgca tcagctccat aggtatcaat aagagctaat aaatctgggc
     1441 tattacctaa ctgtttgctc atttttctac cttgcttatc tcttaccatt cctgtaaaat
     1501 aaacatcttt aaaaggtagt tgttgtttaa actcatatcc tgccataatc attcttgcta
     1561 cccaaaagaa aataatatct tgccctgtaa ctaatgttgt ggttggataa taataattaa
     1621 tttcttcatt attaggttgg ctaattcctt taaaaacttc aattggccaa agccaagagg
     1681 agaaccaagt atccaaaaca tcttcctctt gtgtaatagt ttgtaaattg gaggatggaa
     1741 actgtgtttt aaattggata atagcttctt cttttgttgc agctactaca aaatttccct
     1801 catcatcata ccatgctggt attctttgac cccaccaaag ttgtcttgat atacaccagt
     1861 cttttacatt aatcaaccaa tgcctgtaag tattttttaa tttagaagga tgaaaattta
     1921 ttgtatcatt tagtactgca ttaagtgcct ctggattttt ttccataaac attttcatgt
     1981 tcataaacca ctgcatagaa agcctgcttt ctataattgc tccagttctt tcgcttgtac
     2041 ctacctggct tttatattct tctacttttt ctaactggcc taattcagaa atgtcttttg
     2101 caattatttt tcttagctca aacctgtcaa taccatgata atttttaaca acctcagaag
     2161 gattatcttc taacaattca tctattgtaa attttccatc ttcatctaat gtattgattg
     2221 cttttaattg gtattttctt cctaattcat tatcattttt atcgtgagct ggagttattt
     2281 ttaatgcccc tgttccaaaa gtaacatcta catattcatc tgcaatgatg ggtatttttc
     2341 tgtttataat tggcacaatc acttctttgc caataaggtt tttgtaacgt tcatctgtag
     2401 ggtttacaca aatagccaca tcgcctaaaa tagtttctgg tcttgttgtt gcaaccataa
     2461 taaattgatt agcatcagaa gccattgcgt attttacaaa ataaagttta gagtctgttt
     2521 ctttaaaaat tacttcctca tcgcttagag ctgttttact tgctgggtcc caattaacca
     2581 ttcttaagcc acgatatata atgccatctt tatataattg aatgaatgta ttgataacgc
     2641 ttttataata atcatcatcc attgtaaaag ctgttctatt ccaatctaaa ctgcaaccta
     2701 atttttttaa ttgctgcaaa atgatgccgc catatttttc tttccactcc c
//
LOCUS       DAOQHP010000308         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26129_length_2706_cov_3.322143, whole genome shotgun sequence.
ACCESSION   DAOQHP010000308 DAOQHP010000000
VERSION     DAOQHP010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26129_length_2706_cov_3.322143"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLY81_09025"
     CDS             complement(<1..1317)
                     /locus_tag="PLY81_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF30408.1"
                     /translation="MEQKSFSIKGNIVDVVHKQIYAGEVKVVNGKIASVTPLPKVETD
                     QFILPGFIDSHVHVESSMLVPSEFARLAVVHGTVATISDPHEIANVCGIQGVEFMIEN
                     GKKVNFKFNFGAPSCVPATSFETAGAVLNSNDVKTLLAKEEIIYLSEMMNFPGVLFKD
                     EEVMKKLQAAIDAGKPIDGHAPGLRGEKAKQYIEAFGKNGEIIISTDHECFTKEEALD
                     KLKYGMKIIIREGSAAKNFEALIDLLHDYEDKIMFCSDDKHPDSLAIGHINQLCARAV
                     AKGIDIFKVLKAACLNPIIHYKMNVGLLQAGNDADFIIAEDLKSFKIIQTYIKGKLVA
                     ENGKTFIPSVPVTAINQFNCNEITKTQLQIKLGDYPTVDNKIYVIEALEGQLITNKLL
                     LQGKQENKEWVSNIENDILKMVVVNRYTNAPIAKCFIKNFGLKQGAM"
     gene            1397..2209
                     /locus_tag="PLY81_09030"
     CDS             1397..2209
                     /locus_tag="PLY81_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30409.1"
                     /translation="MKKLFFAVFLSFSVFALNAQTVDEVINRFIEANGGLSKLTSINS
                     LQIESSMNIEQMGSTINVLTIKEKNKLFRIQSSSPMSSEESFTVITDTAGYTFVPAMN
                     SPMGSMDASLTKFKEDELEASSYQKDCEGLFAPLVNYAAKGHTATLDGTEKINGIECD
                     KVNLKLKSGQEMTFYISKANAQVRRLQVSAPIALDMMGMSAMRRSFGGGGERSSRMAN
                     RKIDIDYEKYKLFNGIPFPTKQSVSLGMMQLQLDNLSIKVNEPINARWYQVK"
     gene            complement(2222..>2706)
                     /locus_tag="PLY81_09035"
     CDS             complement(2222..>2706)
                     /locus_tag="PLY81_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF30410.1"
                     /translation="NNSIKINPLVHGGSQERNMRAGTENLYGIIGFAKALELATENYE
                     KDSIYINGLKLFMKELLQKNIEGVNFNGDVDGKSLYTVLSVNFPKTEKSDMILFNLDI
                     NNICASGGSACSSGADAGSHVVGALHNNSHTVTVRFSFCKHNTKEEIETVVEKIKTLI
                     "
BASE COUNT          885 a          456 c          378 g          987 t
ORIGIN      
        1 cattgctcct tgctttaaac caaagttctt aataaaacat ttagcaattg gtgcattagt
       61 atatctattt accaccacca tttttaaaat atcgttttca atattgctta cccattcctt
      121 attttcttgt ttgccttgca ataaaagttt attggttatt agctgccctt ctaaggcttc
      181 tataacataa atcttattat caacagttgg gtaatctccc agctttattt gcaattgtgt
      241 ttttgtaatt tcattacagt taaactgatt aattgcagta acgggtacag atggaataaa
      301 agtttttcca ttttctgcaa ccaacttgcc tttaatatat gtttgaataa ttttaaaact
      361 ttttaaatct tcggctataa taaaatctgc atcattgcct gcttgtaata agccaacatt
      421 cattttataa tgtataattg ggtttaaaca agccgctttt aaaactttaa aaatatcaat
      481 accttttgct acagctcttg cacataattg gttaatatga ccaatggcta aactatcagg
      541 gtgcttatca tcgctgcaaa acattatttt atcttcgtaa tcgtgtaata aatcaattaa
      601 tgcttcaaag ttttttgcag cacttccttc tctaataata attttcattc catattttaa
      661 tttgtctaaa gcttcttctt tagtaaaaca ttcatggtcg gtagaaatta ttatttcacc
      721 attttttcca aatgcttcaa tatattgttt tgctttttct cctctcaaac ctggtgcatg
      781 accatctatt ggctttcctg catctatagc tgcttgtaat tttttcatta cttcttcatc
      841 tttaaataaa acgccaggaa aattcatcat ttcacttaaa taaataattt cttcttttgc
      901 caacaatgtt tttacatcgt tgctgtttaa aacagcacca gcagtttcaa aactggttgc
      961 aggtacgcaa cttggggctc caaaattaaa tttaaaattt actttctttc cgttttcaat
     1021 cataaactca actccttgaa tgccacaaac attggcaatt tcatgagggt cgctaatggt
     1081 tgcaacggtg ccatgtacaa cagctaatct tgcaaattcg ctgggtacaa gcatgctact
     1141 ttcaacatgt acatggctat ctataaaacc aggtaaaata aattgatctg tttctacttt
     1201 gggcaaaggt gttacagatg ctatttttcc atttacaact tttacctcac cagcataaat
     1261 ttgtttatgt actacatcta caatatttcc ttttattgaa aagctttttt gttccataat
     1321 attttattaa aattttattc ttattaaatt aattccaaaa gcaaactaaa tttgagcaat
     1381 aaaatataaa agtataatga aaaaattatt ttttgctgtt ttcttatcat tttcagtatt
     1441 tgctttaaat gcacaaacag ttgatgaggt tataaataga tttattgaag ccaatggagg
     1501 gttaagtaag ttaacatcta ttaatagttt gcagatagaa agttctatga atattgaaca
     1561 aatgggttct actattaatg tacttaccat taaagaaaag aataaattat ttcgtattca
     1621 aagcagtagc ccaatgagca gcgaagaaag ttttactgta attaccgata ctgctggtta
     1681 tacatttgtt cctgcaatga actctccaat gggtagtatg gatgcctctc ttacaaaatt
     1741 taaagaagat gaattagagg cttcctctta tcaaaaagat tgtgaaggct tgtttgcacc
     1801 attggtaaat tatgcagcca aagggcatac agctacttta gatggaacag aaaaaattaa
     1861 tggaatagaa tgtgataaag taaacttaaa actaaaatct ggtcaagaaa tgacttttta
     1921 tatttctaaa gcaaatgcac aagtaagaag gttgcaggtt agtgcaccaa ttgctttaga
     1981 tatgatgggt atgagtgcaa tgagaagatc atttggtggt ggtggagaaa gaagctctag
     2041 aatggctaac agaaaaattg atattgatta tgaaaaatat aagctattta atggcattcc
     2101 ttttcctaca aaacaatcag tttcattagg aatgatgcaa ctacaactag ataatttaag
     2161 tattaaagta aacgaaccaa taaatgcaag atggtatcag gttaaatagc tgaaccaaag
     2221 tttaaataag agtttttatt ttctcaacca cagtttcaat ttcttctttt gtattgtgtt
     2281 tacaaaaact aaatctaact gtaacagtat gactgttgtt gtgtaatgca cctacaacat
     2341 ggcttcctgc atctgcacca ctagagcatg cactgccacc acttgcacaa atattattaa
     2401 tatctaaatt aaaaagtatc atatcgcttt tttctgtttt aggaaagtta acgcttaaca
     2461 cagtatataa actttttcca tcaacatctc cattaaaatt tacaccttca atattttttt
     2521 gtaacaactc tttcataaaa agttttaagc catttatata tatgctgtct ttttcataat
     2581 tttctgttgc taactctaaa gcttttgcaa agccaataat accgtataaa ttttctgttc
     2641 cggctctcat atttctttcc tggctaccgc catgtactaa cggatttatt ttaatactgt
     2701 tattaa
//
LOCUS       DAOQHP010000309         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26176_length_2703_cov_3.188066, whole genome shotgun sequence.
ACCESSION   DAOQHP010000309 DAOQHP010000000
VERSION     DAOQHP010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_2703_cov_3.188066"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="nadC"
                     /locus_tag="PLY81_09040"
     CDS             <1..583
                     /gene="nadC"
                     /locus_tag="PLY81_09040"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNF30411.1"
                     /translation="LTCERLVLNCMQRMSGIATLTKTYTEKLQGFHTQLLDTRKTTPN
                     FRLLEKEAVRIGGGINHRFGLYDMIMLKDNHIDYAGGLENAIDKAWLYATNYKPLLKI
                     EVETRSMEDVVKVCEVGMGKVFRIMLDNFTPEQVLKAVQIINNRFQTEASGGINLKTI
                     QAYAATGVDYVSVGGLIHQATSVDLSLKAVITN"
     gene            697..1638
                     /gene="paaA"
                     /locus_tag="PLY81_09045"
     CDS             697..1638
                     /gene="paaA"
                     /locus_tag="PLY81_09045"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNF30412.1"
                     /translation="MELKKLEEIFQHKIDNEIKIEPKDWMPEAYRKTNIRQISQHAHS
                     EVVGMLPEGNWITRAPSLKRKAILLAKIQDEAGHGLYLYSAAETLGISRDEMIEQLHS
                     GRAKYSSIFNYPTLTWADMGAIGWLVDGAAIINQVMLCRTSYGPYARGMVRICKEESF
                     HQRQGFEILYVLSQGTDAQKAMCQDAINRWWWPSLMMFGPTDAESSNSEQSMLWKIKR
                     KSNDELRQQFVDMIAEQVKVLGMTLPDADLKWNETKKHYDFGAINWNEFWNVVKGNGP
                     CNKQRLNARIAAHQNGAWVREAAIAYAEKQKNKHKAA"
     gene            1640..2050
                     /locus_tag="PLY81_09050"
     CDS             1640..2050
                     /locus_tag="PLY81_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30413.1"
                     /translation="MKLFPLVFLMGLTFISCTNNNKEKTIDNRIALRADTVNILKLTD
                     TAVIFEGTCRGCAFESTTSFKIYDSLSLIKLLSIKTIDNNPPDMDGGNISKEVELVPL
                     QTGNTTIKVYKIFGQETEKEDTMHATLYKIKITN"
     gene            2105..2422
                     /gene="paaB"
                     /locus_tag="PLY81_09055"
     CDS             2105..2422
                     /gene="paaB"
                     /locus_tag="PLY81_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNF30414.1"
                     /translation="MEAGSNASNNNKNIAWPLWEVFIRSKQGLDHKHVGSLHAADEQM
                     AIENARDVYTRRNEGVSIWVVESKYITASNPEEAGELYEPAVNKIYRHPTFYKLPDEV
                     DYM"
     gene            2479..>2703
                     /locus_tag="PLY81_09060"
     CDS             2479..>2703
                     /locus_tag="PLY81_09060"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HNF30415.1"
                     /translation="MQAAKENFLLYLADSTHILSHRNSEWCGHAPILEQDIALTNIAL
                     DLLGQSRNFYQYAAELIGNNATEDSLAYLRI"
BASE COUNT         1045 a          373 c          506 g          779 t
ORIGIN      
        1 attaacctgc gaaagattgg tactaaactg tatgcaaaga atgagtggca ttgctacact
       61 tacaaaaact tataccgaaa aattacaagg ctttcataca caattgttag ataccagaaa
      121 aacaacaccc aattttaggc ttttagaaaa agaagcagta agaattggtg gtggcattaa
      181 tcataggttt ggtttgtacg atatgattat gcttaaagat aaccatattg attatgcagg
      241 tggattagaa aatgctattg acaaagcttg gctttatgca accaactata aaccattatt
      301 aaaaatagaa gttgaaacca gaagtatgga agatgtagta aaagtttgcg aagtgggaat
      361 gggaaaagtt tttagaatta tgttagataa ttttacacct gagcaggttt taaaagcagt
      421 acaaataatt aataatagat ttcaaacaga agcaagtgga ggcataaatt taaaaaccat
      481 acaagcctat gctgcaacag gtgttgatta tgtaagtgtt ggtggtttaa tacaccaggc
      541 taccagtgta gatttaagtt taaaagcagt aattacaaat taatttttac attcaaaatt
      601 atatagctgt tacttattgt agcattctta tatctttaat tttcctattt acattaaagc
      661 aatatactta tttttgtaag tataaaataa tgcgttatgg agttgaaaaa attagaagaa
      721 atttttcagc ataaaataga taatgaaata aaaattgagc caaaagattg gatgcctgaa
      781 gcgtacagaa aaaccaatat cagacaaata agccaacatg cacacagcga agtagtaggt
      841 atgttgccag aaggtaattg gattaccagg gcaccatcct taaaaagaaa agctatttta
      901 cttgcaaaaa tacaagatga agcagggcat ggtttatacc tttattctgc tgcagaaacc
      961 ttgggcatta gccgagatga aatgatagaa caactacata gtggtagagc aaaatattct
     1021 tctatattta attatcctac actaacctgg gccgatatgg gtgcaatagg ttggttggta
     1081 gatggggcag caattattaa tcaggttatg ctttgtcgta cctcttatgg tccttatgca
     1141 agaggtatgg ttagaatttg taaagaagaa agtttccatc aacgtcaagg ttttgaaatt
     1201 ttatatgtgt taagccaagg tacagatgca caaaaagcaa tgtgccaaga tgcaatcaat
     1261 agatggtggt ggccaagttt aatgatgttt ggcccaaccg atgcagagag ttccaattca
     1321 gaacaaagta tgctctggaa aattaaaaga aaaagcaacg atgagcttcg ccaacagttt
     1381 gtagatatga ttgcagagca agtaaaagta ttaggaatga cattgcctga tgctgattta
     1441 aaatggaatg aaaccaaaaa acattacgat tttggtgcta ttaattggaa cgaattttgg
     1501 aatgtggtaa agggcaatgg cccatgcaat aaacaaagat taaatgcaag aattgcagca
     1561 caccaaaatg gtgcttgggt aagagaagct gctattgctt atgcagaaaa acaaaaaaat
     1621 aaacataaag cagcataaaa tgaaattatt tccattagtt tttcttatgg gtttaacctt
     1681 tataagttgt acaaacaaca ataaagaaaa gacaatagat aacagaattg cattaagagc
     1741 agatactgta aatattttaa agcttaccga taccgcagta atttttgaag gaacctgtag
     1801 aggctgtgcc tttgaaagta caaccagttt taaaatatat gatagccttt ctttaataaa
     1861 attactatcc ataaaaacaa tagataataa cccacctgat atggatggag gaaacataag
     1921 caaagaagta gaattagtac ctctacaaac aggcaataca acaattaaag tgtacaaaat
     1981 atttggacaa gaaactgaaa aagaagatac aatgcatgct acattataca aaataaaaat
     2041 aaccaactaa acttttttta tgaataactt aattaaaaaa tatttttatg gcgattatgg
     2101 agaaatagaa gctggcagca atgctagtaa caataataaa aatattgcat ggccactatg
     2161 ggaggtgttt attagaagta aacaaggatt agatcataaa catgttggca gtttacatgc
     2221 tgcagatgag caaatggcta ttgaaaatgc aagagatgtt tatacaagaa gaaatgaagg
     2281 agtaagtatt tgggtggttg aaagtaaata tattactgca agtaacccag aagaagcagg
     2341 ggagttgtac gaaccagcag tcaacaaaat atatcgtcat ccaacatttt ataaattgcc
     2401 agatgaagta gattatatgt aaaaattatt ttagtatcgt acaaatatta atccattttc
     2461 tccataaatt atttaaccat gcaagctgct aaagaaaatt ttttactcta tctagccgat
     2521 tcaacgcata tactttctca tagaaatagt gaatggtgtg gtcatgcacc tattttagaa
     2581 caagatattg cacttaccaa tattgcatta gatttattag ggcagtcaag aaatttttat
     2641 caatatgccg cagaattaat tggtaataat gcaaccgaag atagccttgc ttatttaagg
     2701 ata
//
LOCUS       DAOQHP010000310         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26192_length_2702_cov_3.564035, whole genome shotgun sequence.
ACCESSION   DAOQHP010000310 DAOQHP010000000
VERSION     DAOQHP010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26192_length_2702_cov_3.564035"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLY81_09065"
     CDS             complement(<1..753)
                     /locus_tag="PLY81_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF30416.1"
                     /translation="MQLYNIETENFKLDGGAMFGVVPKSIWNKLNPADENNMCSWAMR
                     CLLIQDGNRLILVDNGIGNKQDAKFFSYYFLHGENTLEKSLAKYGFHKNDITDVILTH
                     LHFDHCGGSVDRIDNKLIPAFKNATYWSNQNHWQWATQPNDREKASFLKENILPIQEN
                     GQLQFINLPNTSLEQTGKLGMSSFNEFIDLRFVNGHTDMMMLPQIKYKNKTIVYMADL
                     LPSAAHIPLPYVMAYDMFPLTTLKEKKSFLQEA"
     gene            complement(740..2047)
                     /locus_tag="PLY81_09070"
     CDS             complement(740..2047)
                     /locus_tag="PLY81_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF30417.1"
                     /translation="MTNSPKKIINAWAMYDWANSAYNLVITSTMFPAYFEAVTKSNNN
                     EVRFLGRTFVNTSLYNYALGFAFLIVAFISPILSSIADYKGNKKSFLRFFCTIGSIAC
                     SGLYFFDKTNVGLGILLMITACVGYWASLVFYNSYLPEIAEEKDRDWVSAKGFSLGYI
                     GSVLLQIICFVLLFKKEWFGISNDSRALQISFLLVGIWWFGFAQKTLNVLPKGIASEN
                     RPEKNIFINGFIELKKVWKILKEMPLLKRFLFAFFFYNMGVQTVMLAATLYGKSELAI
                     PINNLIIAILIIQLVAIPGAILLSKLSAIIGNLQTLVLAVIFWICICIAGYYLPKGNI
                     NLFYVLAVGVGFIMGGIQSLSRSTYAKLMPQLKETTSFFSFFDVTEKIAIVLGMFGYG
                     FVTELAGGSQRNSVLLLAILFIIGLIILLFTIQFQKKQQYATL"
     gene            complement(2139..2609)
                     /locus_tag="PLY81_09075"
     CDS             complement(2139..2609)
                     /locus_tag="PLY81_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30418.1"
                     /translation="MKKNIFYVLFMITFISACNFFGSSYGKRIEISDKVEVYIKGDST
                     TEEDGKKLGTYLDTTWKDLTNKRSFQLIKDKGEYNVSMVVDETKLKEDSTLEESFKLL
                     RLLIAMSVFPENKVNLILTNDTFKPYKTYKDEGKNTMIEEDAIEPVATDTTTSK"
BASE COUNT          962 a          432 c          333 g          975 t
ORIGIN      
        1 agcctcttgt aaaaacgatt ttttttcttt taaggttgtt aacggaaaca tatcgtatgc
       61 cattacatag ggtagtggaa tatgagctgc actaggtagc aaatctgcca tgtaaacaat
      121 agttttgttt ttgtatttta tttgtggcaa catcatcata tcggtatggc catttacaaa
      181 tcttaaatca ataaattcat taaaactact cattcctaat tttcctgttt gctctagaga
      241 agtattgggc aaattaataa attgtagttg cccattttct tgaattggta aaatattttc
      301 ttttaaaaaa gaagcttttt ctctatcgtt tggctgtgta gcccattgcc agtggttttg
      361 attactccaa taagttgcat ttttaaaagc aggtatgagt ttgttatcta ttctatctac
      421 actacctcca caatggtcaa aatgtaaatg agttaaaatt acatcggtaa tatcattttt
      481 atgaaagcca tattttgcta aagatttttc taaagtattt tctccatgta aaaaataata
      541 cgaaaaaaat ttggcatctt gtttatttcc aattccatta tctactaaaa ttaatctatt
      601 gccatcttga attaataaac aacgcatagc ccagctacac atattgtttt catctgcagg
      661 gtttaattta ttccaaatac tttttggcac aaccccaaac atggcacctc catctaattt
      721 aaaattttct gtttctatat tataaagttg catattgctg ttttttttga aattgaattg
      781 taaacaataa tattattaat cctattataa acaaaatagc caataataaa acagagtttc
      841 tttgagaacc acctgccaat tctgtaacaa agccataacc aaacatacct aaaacaatag
      901 caattttttc ggttacatca aaaaaactaa aaaatgaagt tgtttctttt aattgtggca
      961 ttagtttagc ataggtagat ctacttaaac tttgtattcc acccataata aatccaacac
     1021 ctactgccaa aacataaaac aaattaatat tgcctttggg taaataataa ccagcaatgc
     1081 aaatacaaat ccaaaatatt acagccaaaa caagcgtttg taaattacca attattgcag
     1141 ataacttgga taaaagaatt gctccaggta tggccactaa ctgaataatt aatatggcaa
     1201 taattaaatt atttataggt atggctaatt cgcttttgcc atataaagta gcagcaagca
     1261 taacagtttg tacccccata ttataaaaaa agaatgcaaa taaaaacctc ttaagcaaag
     1321 gcatttcttt taaaattttc catacttttt ttaattcaat aaacccattg atgaaaatat
     1381 ttttttctgg cctattttca gaggcgatgc ctttgggtaa aacatttaat gttttttgtg
     1441 caaagccaaa ccaccaaatg cctactaata aaaaagaaat ttgtaatgct ctgctatcat
     1501 tagatatacc aaaccattct tttttaaata ataaaacaaa acaaattatt tgtaataata
     1561 cacttcctat atagcctaaa gaaaagcctt ttgcactaac ccagtctctg tctttttctt
     1621 ctgcaatctc tggtaaatat gaattgtaaa atactaaact agcccaataa ccaacacatg
     1681 ctgtaatcat taatagaata cctaagccaa cattggtttt atcaaaaaag tataaaccgc
     1741 tacaagctat actgccaata gtacaaaaaa aacgaagaaa agatttttta ttgcctttat
     1801 aatcagcaat agaagataaa ataggcgaaa taaatgctac aattaaaaat gcaaatccta
     1861 atgcataatt atataaagaa gtatttacaa atgttctacc taaaaatcgt acttcattat
     1921 tattgctttt tgttacagcc tcaaaatatg ctggaaacat agtggaagta atcactaaat
     1981 tatatgcact atttgcccaa tcgtacattg cccatgcatt aataattttt ttaggtgaat
     2041 ttgtcatgta ggcttttttt gaaatttaaa gataagtaaa caaaaaaaag cattgtattt
     2101 ttttacaatg cctaataatt ttatttaaaa taaaatggtt atttacttgt agtggtatct
     2161 gttgctactg gctcaatagc atcttcttct atcattgtat ttttaccttc gtctttataa
     2221 gttttgtaag gtttaaaagt atcgttggta agtataaggt ttactttatt ttctggaaaa
     2281 acactcattg caattaataa acgtaatagt ttaaaactct cctctaaggt agaatcttct
     2341 tttaattttg tttcatcaac caccatactt acgttgtatt ctcctttatc ttttattaat
     2401 tgaaaagatc ttttgttggt aaggtctttc catgttgtgt ctaaataagt acctaacttt
     2461 ttaccatctt cttcagttgt actatctcct ttaatatata cttcaacttt atcactaatt
     2521 tcaattcttt taccataaga ggatccgaaa aagttacatg cactaataaa tgttatcata
     2581 aatagcacat aaaaaatatt ttttttcata attttttttt tagaggatga aacaaagaaa
     2641 gaattaattt ttggtatcta agataagtgt tattgggtat tttaatttta aaaactatat
     2701 aa
//
LOCUS       DAOQHP010000311         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26702_length_2672_cov_3.353840, whole genome shotgun sequence.
ACCESSION   DAOQHP010000311 DAOQHP010000000
VERSION     DAOQHP010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26702_length_2672_cov_3.353840"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="PLY81_09080"
     CDS             complement(<1..1710)
                     /locus_tag="PLY81_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNF30419.1"
                     /translation="MQLKGLVRFFAIALTLICLYQLSFTWIVHNHENKMEAKAKKWIK
                     DTYASPEAKYSGNVEAKALYEDTLQQLTKVRLQRLLDSTKESKIGPFGLTTYQNAKDK
                     ELLLGLDLQGGMSVTMEVGLDGLIKSLSGYTKDAQFNTAIESAVKLKANSSADLVSLF
                     VQEYKKINPSGKLAPFFATKSSGKIKFDDSDENVVKYLKEQSDNAFANTFRIVNKRID
                     KFGVASPNINQNPAKGIITVELAGVTDHERVRRYLQSTANLQFFEVYTLQDNELSKGF
                     EEANKALQNYLNGVQTVVDTTKAVTTPNTTIDTTQTVKISDVTGTETTKDTSLNKKNV
                     DPIKSLLFGGREHQPTQNRKGGYSYPAALAYVALKDTGLLNQYLALDIVKTKFPSNLV
                     FMYGKAEDENGKVSKTNLNLYAIKTLDNGQAKIEGEHVAEAKQGYDGSRPIIHMNMTK
                     EGTKLWGDLTTKNVDKPIAVVLDNFVYSAPNVNEPITTGSSQISGNYTVKEAQDMADI
                     LQTGKLPAPAKIVQEQIIGPTLGAEAVKGGGMSFLVSFLIIFALMLIYYNTGGWVANI
                     ALVL"
     gene            complement(1865..2209)
                     /locus_tag="PLY81_09085"
     CDS             complement(1865..2209)
                     /locus_tag="PLY81_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30420.1"
                     /translation="MLEKREEDFITFWEKNRLIQKKNTKQFILGLTFGLFIGLSTLML
                     IFSGWYQRATMVATTRLNPFVFIVIIVIIAVFMAYLNRKFKWERHEQQYLELLAKKQK
                     LKEKHTANQEGI"
     gene            complement(2383..2568)
                     /locus_tag="PLY81_09090"
     CDS             complement(2383..2568)
                     /locus_tag="PLY81_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30421.1"
                     /translation="MKKVLALLAIVGFVACNSAENTEVKTDSVAPATPAVDTTTKAVD
                     TTKAVDTTKAAATTPAK"
BASE COUNT          856 a          446 c          376 g          994 t
ORIGIN      
        1 taacactaaa gcaatattgg ctacccaacc accagtatta tagtaaatta acattaaagc
       61 aaaaatgatt agaaaagaaa ccaagaaact cattccgcca cctttaactg cttcagcacc
      121 taaagtagga ccaataattt gttcttgtac aattttagca ggtgcaggta atttaccagt
      181 ttgtaaaata tctgccatat cctgtgcttc ttttacagta taatttccag aaatttgaga
      241 gctaccagtt gtaattggtt catttacatt tggagcagaa taaacaaaat tatctaatac
      301 aactgctatt ggtttatcta cattttttgt tgttaggtcg ccccaaagtt tagtaccttc
      361 tttagtcata ttcatatgaa taataggacg actaccatca taaccttgtt tagcttctgc
      421 tacatgttca ccttctattt ttgcttggcc attatctaaa gttttaatag catataaatt
      481 caaattcgtt ttagatactt tgccattttc atcttctgct ttgccgtaca tgaaaactaa
      541 attgcttggg aatttagttt ttacaatatc taaagctaaa tattggttta ataaacctgt
      601 atccttcaat gcaacataag ctaaagctgc aggataagag tagccccctt ttctgttttg
      661 agttggttga tgctctctac ctccaaacaa caacgactta attggatcta catttttctt
      721 atttaaggaa gtgtcttttg ttgtttcagt tcctgttaca tcagaaattt ttacagtttg
      781 tgttgtatca atagttgtat ttggtgttgt aactgcttta gttgtatcta ctacagtttg
      841 tacaccattt aaataatttt gaagagcttt attagcttcc tcaaagcctt tacttaattc
      901 gttatcttga agtgtatata cttcaaaaaa ctgaaggtta gctgtagatt gtaagtatct
      961 tctaactctt tcatggtcag taacaccagc taattctaca gtaataattc cttttgcagg
     1021 attttgattg atattaggag atgctactcc aaatttatca atacgtttat ttactattct
     1081 aaatgtatta gcaaaagcat tgtctgattg ttcttttaaa tatttaacaa cattttcgtc
     1141 gctatcatca aacttaattt taccgctact tttagttgca aagaatggag ctaatttacc
     1201 actagggttt atctttttat actcttgaac aaaaagcgaa actaaatcgg cactgctatt
     1261 ggcttttaat ttaacagcac tttctattgc agtattaaac tgtgcatctt tagtatagcc
     1321 agataatgat tttattaatc catctaaacc aacttccatt gttacactca taccaccttg
     1381 caaatccaaa cctaataaaa gttctttgtc ttttgcattt tgataagtag ttaaaccaaa
     1441 tggaccaatt ttagattctt tggtactatc taataaacgt tgtaaacgaa ctttggttaa
     1501 ttgttgaagt gtatcttcat acaaagcttt tgcttcaaca ttaccactat acttagcttc
     1561 tggcgatgca taagtgtctt ttatccattt ttttgctttt gcctccattt tattttcatg
     1621 gttatgaact atccatgtaa atgaaagttg atacaaacaa attaaagtaa gtgcaatagc
     1681 aaaaaatcta actaaacctt tgagttgcat aataagtaac gggttttaca ttttttttag
     1741 agtgggcaaa gataggggaa tgatatatgt aaaaaacagc aaaaaaatca atatttaaaa
     1801 tttgttattt acttattcca ctttccttta ttaaaaattg agtataaaat gggaaaatat
     1861 ctatttaaat gccttcttgg ttagcagtat gtttttcttt taatttttgt ttttttgcta
     1921 aaagttctaa atactgttgt tcatgtctct cccatttaaa ctttcggttt aaataagcca
     1981 taaaaacagc aattattaca ataattacaa taaaaacaaa agggtttaac cttgttgtag
     2041 ccaccattgt agctctttga taccagccag aaaaaataag cattaaagta gataagccta
     2101 taaataaacc aaatgttaag cctagaataa attgtttggt attttttttc tgaattaacc
     2161 tattcttctc ccaaaaggta ataaaatctt cttctcgttt ttctaacata caggcaaaat
     2221 taaaagaaac gaaataaaaa agccaccttg taaaaacaag acggctttta acgattgctt
     2281 gccatatgaa acgtatttat tttttgtgcc ctcagttgca agtcaactgc acttttctat
     2341 atcacttgca caaaatcaag acatttcgtc taaataaggt aattatttag caggagtagt
     2401 agcagcagct ttagttgtat caacagcttt agttgtatca acagctttag ttgtagtatc
     2461 tactgcagga gttgctggag caacgctatc agtttttact tctgtgtttt cagctgagtt
     2521 acaagctacg aaacctacga ttgctaataa tgcaagtact tttttcatga tttttgtttt
     2581 tttatgagtt aaatttcatg ttaataccca actttgaaaa aaggtaaccc aactttttta
     2641 aaaaaattta aatatttttt tttactcgtt tg
//
LOCUS       DAOQHP010000312         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26709_length_2671_cov_4.003823, whole genome shotgun sequence.
ACCESSION   DAOQHP010000312 DAOQHP010000000
VERSION     DAOQHP010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26709_length_2671_cov_4.003823"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLY81_09095"
     CDS             complement(<1..619)
                     /locus_tag="PLY81_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30422.1"
                     /translation="MKKQHWLLSLVCLLISFITKELIGQIQVTANNLPIYQDGKLYVK
                     IKLQSSVIVVSKDSITNDTGDSIKTVFARYNVTQTEQPFTILNDINLNKTYRLTFSDI
                     INVENFITDLQQISAVEYSEKVPAIYTTAVPNDPMQPYHLPLVNSNAASNIHISSGNA
                     IVAIVDDAVLTTHQDLAANVGILNRDVADLDNNPNPPLTGPTAVFN"
     gene            1035..1820
                     /locus_tag="PLY81_09100"
     CDS             1035..1820
                     /locus_tag="PLY81_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30423.1"
                     /translation="MSTFFETGHAKNVANLLKLNQQIATYGVAYNPSNNAIKAAVLAT
                     LYTTANSKLTAVNNLFTTWKNATNARELAFEPLAKQATQILASLQSLGIVQQTIDDFE
                     FQVKKFRSSGSKLTKADSGKVIAPDTPDPNAPVTTVETTSISTSQQSFDNKLQHFEKM
                     ILILQSVPTYNPNEVALKVTSLQTQLTNLTTLNNAANTSYANLKAARIDRNNIFYTKG
                     TGMLDLVKLTKAYIKSLYGASSPQYHAVSSLKFVRVIPKQILH"
BASE COUNT          936 a          489 c          349 g          897 t
ORIGIN      
        1 cgttaaatac tgccgttggt cccgttaatg gcggattcgg attattatct aaatcggcaa
       61 catctctgtt caatattcct acattcgctg ccaagtcttg gtgagttgtt aatacagcat
      121 catctacaat agcgacaatg gcattaccac tagaaatatg aatattagat gcagcattag
      181 agttaaccaa aggtaaatga tagggctgca ttggatcatt tggcacagct gtagtatata
      241 ttgcaggtac tttttccgaa tattctacag cacttatctg ttgcaaatct gtgataaaat
      301 tctcaacatt tattatatca gaaaacgtta atctataagt tttattaagg ttaatatcat
      361 ttaagatagt aaatggctgc tctgtttgtg tgacgttata tctagcgaac acagttttta
      421 tactatctcc agtgtcattt gtaatagaat cttttgatac aacaataaca cttgattgca
      481 acttgatttt tacatataat ttaccatctt gatagattgg caaattatta gccgtaactt
      541 gtatttgtcc aattaactcc tttgtgataa aggaaattaa taggcatact aaactcaata
      601 accaatgttg ttttttcata atttatattt ttaagtataa gcgaattttt aactttattc
      661 ttaatatcaa aattttagtc aaagaaattg cagaaatata tattaaatat aatcttctgt
      721 tcctgtagat tttatatttt ttaaatgtaa tacaaataac taaagtaata agcttaaagt
      781 tttcattgtt aaaaatcatt attaccactt attctcaaaa ttaagccact tacaaagtgg
      841 cttttttgtt ttaacaaaac tttagaattg taccatacca tttggcacag tgtttcgtta
      901 taccccaaca acaaacaaaa aagatatgcc acctaaaatt aattaaacac ccgaactctt
      961 tctcttcctt gctcgttttc ttaaagtaaa gcagaacatc gaaataataa ttaaacaatt
     1021 taatacttaa taaaatgtca acattcttcg aaacaggtca cgctaaaaat gtagctaacc
     1081 tgttaaaatt aaaccaacaa attgctactt atggtgtagc ttataatcca agtaataacg
     1141 caattaaagc ggcagtatta gcaacattgt acacaactgc taattctaaa ttaactgctg
     1201 taaataactt atttacaact tggaaaaacg ctacaaatgc tcgcgaatta gcattcgagc
     1261 ctttagcaaa acaagcaact caaattctag cttcattgca aagtttgggt atcgttcaac
     1321 aaactattga cgactttgaa tttcaagtaa agaagttccg tagctcaggt tctaagttaa
     1381 ctaaagcaga ttcagggaaa gtaattgcac ccgacacacc cgacccaaat gctccagtta
     1441 caacagtcga aacaacttct atttctacat cacaacaaag tttcgataat aagttacaac
     1501 actttgaaaa aatgatttta atcttacaaa gtgttcctac ttataaccct aacgaggtag
     1561 cattaaaagt aacatcatta caaacacaat taactaactt aacaacctta aacaatgcag
     1621 ctaatacgag ctacgcaaat ttaaaagccg ctcgtattga tagaaataac atcttctata
     1681 caaaaggtac aggtatgtta gacttagtta aattaactaa agcgtatatc aaaagtttgt
     1741 acggagcatc aagcccacaa taccacgcag ttagttcatt aaaatttgtt agagtaatac
     1801 ctaaacaaat cttgcactga gtttgtcgaa gtgtttatct cttccttaaa aatccaacat
     1861 cacaagttgg atttttttat gcaattaatc caaatcaaac tttacaatgt ttagtttcat
     1921 aaaccttata tttttagctg tctgtcgaat aacttcttac aaaatgttat ttgatagttc
     1981 aataatgtaa gccaatagtt caaccaataa attatcctta gacatcaact ttatgtttat
     2041 gtcaatccat ttttaattta attcaatcct ccaaacattc ttgtcagtaa acaaaaggtt
     2101 ttctttaatc aataaaactt cttggagcaa caatttttat tttaattcaa actgcgaact
     2161 attttcttgc tactgtcatt agttttcttc gacttttaaa aattataatt cgttaaaact
     2221 tatctttatt taagtatttc aaaaaaatac ttcaaatcgt gcagacaata tgtctagctg
     2281 tacaatcttt acatgtaaat aaacttcgtt tattccgacc tgtgttttct attctttgtg
     2341 ctaacaaatc tttactggca acattttaaa attaagacaa ctaaaccgcc acaacttgtc
     2401 gacctttgtt tataaagcta agtgtgtcga cctgtgtgta atctccccaa cattttgcaa
     2461 gcacatttgg tcgcttcttt ttaaaggaag taatttattc cttgaaccaa ataagcttgc
     2521 aaaatggctc acgctcctca ctagacgtaa ttagaactgg cggttttcca aaccgctttc
     2581 cctatcacac aataaaaaac taaaaactgt gttccaaaaa aaagcattcg gcaaccaact
     2641 aaccgcatag acgcgttact gcttagttcg g
//
LOCUS       DAOQHP010000313         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_26713_length_2671_cov_3.561927, whole genome shotgun sequence.
ACCESSION   DAOQHP010000313 DAOQHP010000000
VERSION     DAOQHP010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26713_length_2671_cov_3.561927"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PLY81_09105"
     CDS             complement(<1..1329)
                     /locus_tag="PLY81_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30424.1"
                     /translation="MFTNKILITITISIFSCITNINAQKIAYSEPDKEEARNGNFEII
                     GKLNNQFLIYKNFRNSYYIYSYNNQMELVEKNKLDFLPDRIIDANFITYQSFAYMFYQ
                     YQKRNIIYSMGIKIDANGKNISEPIQLDSTEVNFNTNNKIYTILVSENKQKIMSLKIN
                     NKSEKYHIIKTILLDNNLNLTEENFTSIDMPERNDFLTGFQLDNDGDLVFVRAAGNAQ
                     NNNINHLSLITKHANSNQLTTSILPIKNLYLDNITVKIDNYNKRYLTTSFFSKQKRGN
                     IEGLYCYVWDKTNSKAIMNNTSTFSEELRNEAKGESTTKSAFNDYYLKNIITRIDGGF
                     IVIAESIYSSYRGGMNNRWDNWNNSPYWGGMSGGYYTYGYASRMGSPWYPWGDNNSFN
                     NITRYYADNILMLSYDSSAKIDWTNVIIKSQYDDDSDEKLNYTAINTGTDI"
     gene            complement(1384..>2671)
                     /locus_tag="PLY81_09110"
     CDS             complement(1384..>2671)
                     /locus_tag="PLY81_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNF30425.1"
                     /translation="ILGASEQINESEKLKDFIIMGELSLDGSIQPIKGALPIAIQARK
                     EGFKGLIVPKQNAREAGMVNNILVYGVEHINEVVNFFKDEQTLQPTIVNTREDFFNAQ
                     FEFEVDFNDVKGQENIKRALEIAAAGGHNAILIGPPGAGKTMLAKRLPSILPPLSLLE
                     ALETTKIHSVAGKLAENTSLLSKRPFRSPHHTISDVALVGGGGIPQPGEISLAHNGVL
                     FLDELPEFKRTVLEVMRQPMEERKVTISRAKVAVDFPANFMLIASMNPCPCGFYNHPE
                     KECTCPAGAVQKYLNKISGPLLDRIDLHVEVTPVAFNELSNHKVIGESSEQIRNRVIA
                     ARELQAQRYQQHTGIYANAQITSKMLREICVLDNVSEALLKKAMERLNLSARAYDRIL
                     KVARTVADLATSSSIKPEHIAEAIQYRSLDRESWAG"
BASE COUNT          819 a          460 c          367 g         1025 t
ORIGIN      
        1 tatatccgta cctgtattta ttgctgtata attaagtttt tcatcagagt catcatcgta
       61 ttgagattta ataataacat tagtccaatc tattttggca gaagaatcgt agcttaacat
      121 taaaatatta tctgcatagt atcgtgtaat attattaaag ctattattat cgccccaagg
      181 ataccaagga gaacccattc tactagcata tccataagta taataaccac cagacatacc
      241 tccccaatat ggagagttgt tccaattatc ccatctgttg ttcatgccac ctctgtagga
      301 ggaataaata gattctgcaa ttactataaa cccaccatct attcttgtaa taatattttt
      361 taaataataa tcattaaatg ccgattttgt ggtactctct cctttagcct catttcttaa
      421 ttcttcagaa aaagtagaag tgttattcat aatagcttta ctattagttt tatcccaaac
      481 ataacaatac aagccttcta tattgcctct tttttgttta ctaaagaaag aagtggttaa
      541 atatctttta ttataattat caatttttac agtaatatta tctaggtata aattttttat
      601 gggtaaaata cttgtagtta actggttact attagcatgt ttggtaatta aggataaatg
      661 attaatgtta ttattttgag cattacctgc tgctctaaca aatactaaat ccccatcatt
      721 gtctagttga aaacctgtta aaaagtcatt tctttcgggc atatcaatac tggtgaagtt
      781 ttcttctgta aggtttaggt tattatccaa taaaatggtt ttaataatat ggtatttttc
      841 gcttttatta ttaattttta ggctcattat tttctgcttg ttttcactta ccaaaatggt
      901 ataaatttta ttattagtat taaaatttac ttccgttgaa tcaagttgta taggttccga
      961 aatgttttta ccattggcat ctatcttaat acccatagag tatatgatat ttcttttttg
     1021 atattggtaa aacatatacg caaaactttg gtaggtaata aagttggcat caataattct
     1081 atcgggtaaa aagtctaatt tgtttttttc aactaactcc atttggttgt tgtaagaata
     1141 tatgtaataa ctatttctaa agtttttata tattaaaaac tgattgttta gtttgccaat
     1201 tatttcaaaa ttgccatttc ttgcttcttc tttatcaggc tctgagtaag caattttttg
     1261 agcatttata ttagttatac aactaaaaat tgaaatagta attgtaatta aaattttatt
     1321 cgtaaacata catgtaactt taaatttcaa atgtaatagt ttaaatttat ttgaacataa
     1381 caactatcct gcccaacttt ctctgtccag gcttctatat tgtatagctt ctgcaatatg
     1441 ttctggtttt attgaactgc ttgttgctaa atctgcaact gtacgagcta cttttaaaat
     1501 tctatcataa gctctggcac ttaagtttaa cctttccatt gcttttttta acaatgcttc
     1561 gctaacatta tctaacacac aaatttctct taacatttta ctggttattt gtgcattagc
     1621 ataaatgcct gtatgttgtt ggtatctttg tgcttgtagc tctctagctg ctataactct
     1681 gttccttatt tgttctgaac tttcgccaat aactttatgg ttgcttaact cattaaatgc
     1741 tactggtgtt acttctacat gcaaatcaat tctgtctaat aatggtccac ttattttatt
     1801 taaatatttt tgcactgcac cagccgggca agtacattct ttttccggat gattgtaaaa
     1861 gccacaaggg caggggttca tacttgcaat aagcataaaa tttgcaggaa aatcaacagc
     1921 aactttagct ctggaaatag taacttttct ttcctccatt ggctgcctca ttacttctag
     1981 aacagttctt ttaaattctg gaagttcatc taaaaacaat actccattat gagccaaaga
     2041 aatttcgcca ggttgtggta ttccgccacc accaacaagt gcaacatcac taatggtatg
     2101 atgtggactt ctaaaagggc gtttagaaag taaagaagta ttttctgcaa gtttaccagc
     2161 aacactatgt attttagttg tttctaaagc ctccaataaa cttaaaggag gtaatatact
     2221 tggtaatctt ttggcaagca tagttttacc agcaccagga gggcctatta gtatagcatt
     2281 atgaccacct gctgcagcta tttctaatgc ccttttaatg ttttcttgcc cttttacatc
     2341 gttaaaatca acttcaaatt caaactgtgc attaaaaaaa tcttctctgg tatttacaat
     2401 tgttggttgt aaagtttgct catctttaaa aaaattaact acttcattaa tatgttctac
     2461 accatacacc aaaatattat tcaccattcc tgcctctctt gcattttgct tgggaacaat
     2521 tagaccttta aagccttctt ttcttgcttg tatggcaatg ggtaaagcac ctttaatagg
     2581 ttgtatagag ccatctaaac tcaattcgcc cattataata aaatctttta gtttttcaga
     2641 ctcattaatt tgttcacttg cacctaaaat t
//
LOCUS       DAOQHP010000314         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_27070_length_2651_cov_3.815100, whole genome shotgun sequence.
ACCESSION   DAOQHP010000314 DAOQHP010000000
VERSION     DAOQHP010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27070_length_2651_cov_3.815100"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /gene="gpmI"
                     /locus_tag="PLY81_09115"
     CDS             <1..1545
                     /gene="gpmI"
                     /locus_tag="PLY81_09115"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNF30426.1"
                     /translation="IKSKYLYVMSNKVILIIMDGWGLGKINSSDAIQHANVPFVNSLN
                     SQYPNSTLVTCGEAVGLPDGQMGNSEVGHLNLGAGRIVYQELQRINVAIKTGELAANQ
                     VLLNSINYVKKNNKALHFIGLVSNGGVHSHINHLKALIDIANNNGVEKIFIHAFTDGR
                     DCDPKSGIGFIKDLSDYIKNTNTKIASITGRYYAMDRDKRWERVQLAYNALVHGQGEL
                     SNNLVTTIQQNYDAGITDEFLKPIINAEVDINEACIKNDDAVICFNFRTDRCREITMA
                     LTQQNIEEQKMQKLNLHYTTITEYDATYKNVQVMFDNDNLNNTLGEVLALNGKKQIRI
                     AETEKYPHVTFFFSGGREKEFEGETRMIVPSPKVATYDLQPEMSAIGITDKLIPEIEK
                     ETEDFICLNFANPDMVGHTGVFSAVVKAVETVDNCVKRIVTTALKHGYTILLTADHGN
                     ADYMINEDGSPNTQHSLNLVPLFLIDAHKKIHLKNGKLGDIAPTILTLMQLPIPKEMT
                     GDVLIY"
     gene            1564..>2651
                     /locus_tag="PLY81_09120"
     CDS             1564..>2651
                     /locus_tag="PLY81_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF30427.1"
                     /translation="MTFKDTFLISFRTVRSNKLRTGITVAIIAFGIMALMGIITAIQA
                     MEHSLRESFSFMGSNAFSIRHKELTVRMGGPNNESTKHDKRKVKEKKSNLNKVITKEE
                     AEYFKNNLHYPAQVSIYRRGGGSLECSYDNKKTNPITTLFGGDENYLAVNGYTIVAGR
                     NLNSIDVNTGRSVCLIGSNTAKTLFGSNPERSIDKIIRVAGLPYRVIGLLKEKGSSGF
                     LRQDDVVITSYVNVSKLSTASSSFLIGVMVKNVSEIEVAQDEAIAAFRSVRKLHPAEA
                     DNFAIEKSDRIAEMFIGFLSGISGSAGAIGMITLIGAAIGLMNIMLVAVNERTREVGL
                     IKAIGGKSKNVRMQFLMESMIISLMGAA"
BASE COUNT          987 a          351 c          511 g          802 t
ORIGIN      
        1 attaaaagca aatatcttta cgtaatgagc aataaagtaa ttttaattat catggatgga
       61 tggggtttag gtaaaataaa ttcgtccgat gccattcaac atgccaatgt accttttgtt
      121 aattctttga atagccaata ccctaatagt actttagtaa cctgtggcga agctgttggt
      181 ttgcctgatg gacaaatggg taacagcgaa gttgggcact tgaatttagg tgctggcaga
      241 attgtatatc aagaactcca aagaattaat gtagccataa aaacaggcga acttgctgcc
      301 aaccaggttt tattaaattc tattaattat gtaaaaaaaa ataataaagc tttacatttt
      361 attggcttgg ttagtaatgg tggtgtacac tctcatatca atcatttaaa agctctaatt
      421 gatattgcaa ataacaatgg ggttgaaaaa atatttatac atgcatttac cgatggcaga
      481 gactgtgacc ctaaaagtgg aataggattt attaaagatt taagtgatta tattaagaat
      541 accaatacaa aaatagcttc aataacaggt aggtattatg caatggatag agataaaaga
      601 tgggagagag tgcaactagc ttacaatgca ttagttcatg gtcaaggcga actaagcaat
      661 aatttagtaa ccactattca acaaaattat gatgcaggta ttacagatga gtttttaaaa
      721 cctattatta atgcagaagt ggatattaat gaagcctgta taaaaaatga tgatgctgtt
      781 atatgcttta attttagaac agaccgttgc agagaaatta caatggcact tacccaacaa
      841 aatatagaag aacaaaaaat gcaaaaattg aatttgcatt acacaaccat tacagaatac
      901 gatgctactt ataaaaatgt gcaagtaatg tttgataatg ataatttaaa caatacactt
      961 ggcgaagtat tagctctgaa tggaaaaaaa caaattcgta ttgctgaaac tgaaaaatat
     1021 ccgcacgtaa cttttttctt tagtggaggc agagaaaaag aatttgaagg agaaaccaga
     1081 atgattgttc catcgcctaa ggtggcaact tatgatttac aaccagaaat gagtgcaata
     1141 ggaattacgg ataaactaat acctgaaatt gaaaaagaaa ccgaagattt tatatgcttg
     1201 aattttgcaa acccagatat ggtagggcat acaggcgttt ttagtgctgt ggtaaaagct
     1261 gtagaaacag tagataattg tgtgaaaaga atagttacta cagcattaaa acatggctat
     1321 accattttac taacagccga tcatggcaat gcagattata tgattaatga agatggcagc
     1381 cctaacacac aacactcttt aaatctggtt cctttatttt taattgacgc tcataaaaaa
     1441 atacacctaa aaaatggaaa attaggcgat attgcaccaa ccattttaac attaatgcag
     1501 ttacctattc caaaagagat gactggtgat gtattaatct attaataata tttttttatt
     1561 taaatgactt ttaaagatac ttttcttatt tcgtttagaa cagttcgtag caataaacta
     1621 cgtacaggta taactgttgc aattatagct tttggtatta tggccttaat gggcattata
     1681 actgcaattc aggcaatgga gcatagttta agagaaagtt tttcattcat gggatccaat
     1741 gcttttagta ttaggcataa agaacttact gttagaatgg gtgggccaaa taatgaaagt
     1801 accaaacatg ataaaagaaa agtaaaagaa aaaaaatcga acttaaataa agtaatcacc
     1861 aaagaagaag cagagtattt taaaaataat ttgcactacc ctgcacaagt tagtatttat
     1921 agacgaggag gtggcagttt agaatgtagt tatgataata aaaaaaccaa ccctattaca
     1981 actttgtttg gtggcgatga aaattatttg gcagtaaatg gttataccat tgttgctggc
     2041 agaaatctta atagtataga tgttaataca ggaagaagtg tttgcttaat aggcagtaat
     2101 actgctaaaa ccttatttgg ctctaatcct gaaagaagta ttgataaaat aattagggtg
     2161 gcaggcttac cttatagggt tattggttta ttaaaagaaa aaggctctag tggttttcta
     2221 agacaagatg atgtagtaat aacttcttat gtaaatgtat caaaactatc caccgcatct
     2281 tcttcttttt taataggggt aatggtaaaa aatgtaagtg aaatagaagt ggcacaagac
     2341 gaggcaattg cagcatttag aagtgttaga aaattgcacc ctgccgaagc cgataatttt
     2401 gccattgaaa aaagtgatag aattgccgaa atgtttattg gatttttaag tggcatttcg
     2461 ggtagtgcag gtgctattgg aatgattaca ttaatagggg cagctattgg gctaatgaat
     2521 ataatgttag tagctgtaaa tgaacgaaca agagaagtag gattaattaa ggctattgga
     2581 ggtaaaagta aaaatgtacg aatgcaattt ttaatggaaa gtatgattat aagccttatg
     2641 ggtgcagcat t
//
LOCUS       DAOQHP010000315         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_27325_length_2636_cov_3.635800, whole genome shotgun sequence.
ACCESSION   DAOQHP010000315 DAOQHP010000000
VERSION     DAOQHP010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27325_length_2636_cov_3.635800"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..2603)
                     /locus_tag="PLY81_09125"
     CDS             complement(186..2603)
                     /locus_tag="PLY81_09125"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNF30428.1"
                     /translation="MSYPTKGYEQEVVQQQASNNQLDAKKLIYKLIGILPWVIISLAI
                     SIFAANLYLRYTPKLFRVSGNLLIKDEDQSSYRSLKDLGIIPGNIDVPNQMQILNSYT
                     LTERVIDSLKLRLKLRQEGRVISSELYGKTMPFKINVVEEYPNTKASAYHINLTRENF
                     IISGAEGQKKYTFGDTVDISYGKIILERNPLVKINPNGYQLTINDLESEVRALRSKIT
                     VVQLTENGGILEIATNEELPDRGIDIINKLMEVYDNAGLDDKNIVAKKTIQFLNDKLG
                     NIAKDLDSIEIKIEQFRKDNKVPSFSEAGSAFFAEYTGLDKNKKILVTQSAMLDHLES
                     YITNFRNTNDIIPPTFGQTDVILLGLIETHNTTVLAKQKLELISKPTDSHLQNMIESI
                     KGIRQNILRAIRNIRVANQTQLQNYESNYSLLESKLQSIPEKQRIEMRLERDRVVKQE
                     IYKDLLKRKEETELSLASNINNTRIVDYAQNYGPKSPNIATARSVAILFGILIPILII
                     VIKDFFNTKIQDRKEIEQATAIPILGELSYEKDKKNVVIENKSRSPIAEQFRLIRTNL
                     QYMVPDKPLQSLLVTSFMSGEGKSFVSLNLASSLAITGAKTVILEFDLRKPKLSKYLE
                     LSNDIGLSNFIIKDMNIDEIIQEVPNRENLFVISSGPIPPNPAELLLSQRTVKLFEQL
                     ATRFDYIIIDTAPVGLVTDALLLEKFVDLSLFILRHKYSFKAEIPFVDKLYRDKKFKN
                     MGIIVNGIKNEGSGYGYGYGYGYGYGYGYGYGYGYGYGYGYGYGYYVQDKKKGFFKRL
                     FSGFKKD"
BASE COUNT          805 a          448 c          352 g         1031 t
ORIGIN      
        1 ggacaccagc ttacaatatt ttttcttgtg agttgtgtat ctactttttt ttcccatctt
       61 ggtttagtat aaataacata ccattttttt tctttgctaa gcatactgat aactttttac
      121 agctccgcca cctcactcac agttaaaaat taagagagtg aacaatgttt aaattgcgaa
      181 aagcattaat cttttttaaa tccgctaaat aatcttttaa agaagccttt ctttttatct
      241 tgaacataat agccataacc ataaccataa ccatagccat agccatagcc ataaccataa
      301 ccatagccat agccatagcc atagccataa ccatagccat aaccacttcc ttcattttta
      361 ataccattca caataatacc catgttttta aatttcttat ctcggtataa tttatcaaca
      421 aaaggaattt cggctttaaa agaatattta tgacgtaaaa taaataagct taaatcaaca
      481 aatttttcta gtaataatgc atccgtaacc aagcctactg gtgcagtatc tataataatg
      541 taatcaaatc ttgtagctaa ttgttcaaat aattttactg ttctttgact taataataac
      601 tctgcagggt tgggtggaat agggcctgaa cttataacaa ataagttttc tctattggga
      661 acttcctgaa taatttcatc aatattcata tccttaataa tgaaattaga aaggcctata
      721 tcgttagaga gttctaaata tttacttaat tttggttttc ttaaatcaaa ttctaatata
      781 actgttttgg cacctgttat agctaaacta cttgccaagt ttaacgatac aaaactttta
      841 ccttcgccac tcataaatga agttaccaat aaactttgta aaggcttatc gggtaccata
      901 tactgcaaat tagttcttat aagtctaaac tgttcggcta ttgggcttct gcttttgttt
      961 tcaattacta cattcttctt atctttttca taagaaagtt ctcctaaaat tggtattgct
     1021 gtggcttgtt caatttcttt tctgtcttgt attttagtgt taaagaaatc tttgataact
     1081 attattaata taggaattaa tatgccaaat aaaatagcta cacttcttgc agttgctata
     1141 ttggggcttt tagggccata gttttgtgca tagtcaacaa tacgtgtatt attaatgttt
     1201 gaagctagtg agagttcggt ttcttctttt ctttttaaga ggtctttata aatttcttgt
     1261 tttactactc tatccctctc taatcgcatt tcaattcttt gtttttctgg aatgctttgc
     1321 aatttagatt ctaataggct gtaattgctt tcgtaattct gtaactgtgt ttggttagca
     1381 actctaatat ttctgattgc tcttagaata ttttgcctaa ttcccttaat actttcaatc
     1441 atattttgta aatgagaatc ggtaggctta ctgattaatt ctaatttttg ctttgccaat
     1501 acggttgtat tgtgtgtttc aattaaccct aataaaataa catctgtttg tccaaatgtg
     1561 ggtggaataa tatcattggt atttctaaaa tttgtaatgt aagattctaa atgatctaac
     1621 attgcacttt gtgtaactaa tattttcttg ttcttatcaa gccctgtata ttctgcaaaa
     1681 aatgcacttc cagcttcgct aaaagagggt actttgttgt cttttctaaa ttgttcaatt
     1741 ttaatttcaa tactatctaa atcttttgca atgtttccta atttatcatt taagaattga
     1801 atggtttttt tagcaacaat atttttatca tctaatcctg cattatcata tacttccatc
     1861 aatttattaa taatgtcaat acctctatct ggcaactctt cattggtagc aatttcaaga
     1921 ataccaccat tttcagtaag ttgaacaaca gtaattttag atcttaaagc cctcacttca
     1981 gactctaaat cattgatggt aagttggtat ccattggggt ttatttttac taaagggttt
     2041 ctttctaaaa ttatttttcc ataagaaata tcaaccgtat caccaaaagt gtatttcttt
     2101 tgcccctctg cacctgaaat aataaaattt tctcttgtta gattaatgtg gtaagcagaa
     2161 gcctttgtgt ttgggtactc ttctacaaca tttattttaa agggcattgt tttgccatac
     2221 aattctgaag aaataaccct accttcttgt ctaagcttga gccttagttt taaagaatct
     2281 ataactcttt ccgttaatgt ataagagttt aaaatttgca tttggttagg tacatcaata
     2341 tttcctggaa taatgcctaa atcttttaga gatctataac ttgactggtc ttcatctttt
     2401 attaataaat taccactcac tctaaatagt tttggagtgt atcttaaata taagttagca
     2461 gcaaaaatgc ttattgctag agaaataatt acccaaggca aaatgccaat aagcttataa
     2521 ataagctttt tagcatctaa ttggttgttg cttgcttgtt gctgcacaac ctcttgttca
     2581 taaccttttg ttgggtaact cattaattat tttactttct taatattaaa tacact
//
LOCUS       DAOQHP010000316         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_27813_length_2608_cov_4.066980, whole genome shotgun sequence.
ACCESSION   DAOQHP010000316 DAOQHP010000000
VERSION     DAOQHP010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27813_length_2608_cov_4.066980"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="PLY81_09130"
     CDS             <1..2043
                     /locus_tag="PLY81_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF30429.1"
                     /translation="DTDGDSLAYKLSAAYDGQDGPNPSPNPPPPSVLNPVSLNYPAPF
                     SSNNPFGTGASINPKTGIISGKAPPIAGKYVVCVTVEEWRNDTLINEHRKDFILRVAN
                     CSSVKPNAGPDDRTCDGFTYFFENQSPNPAIISYVWSFGDGNTSTVPTPTYTYSDTGK
                     YLIKLKVTATGGCQDSSYKYIYAFPGFKPGINTVGTCVVSPINILDATTTVYGTVNSW
                     RWNFGDLTTVADTSNKKDTAWKYPAPGTYNISLIVTNTKGCLDTVNKNVIVKDLPTIS
                     LPFKDTLICSIDTLPLIANTTGNISWAPNYNIISPTSLNPSVYPKDTTTYVITVNDNG
                     CISKDSIKVNVLDFISVTAGPDSTVCLTDTFRLSTISHALSYVWSASTGEIVPQVKFP
                     LVKPTAITKYYVTANLGKCQDYDTIVITPIPYPKVAINAINSICYGDSITLSTNNFIG
                     SSFYWSPSTFITNANSLSPTVKPTDTTFYIITVNDTLGCPKPFSDSIQVNVIPKVVVF
                     AGNDTVVVKNQPLQLQAVTSPIPNNSTYVWSPSTGINNTTIFNPIATLNINSDSIKYK
                     VTVKRPEGCIGSDELVVKIFKTQPDIFIPTAFTPNNDGKNDILKPIPVGISQIDFFRI
                     YNRFGQLIYETKEYLKGWDGNVNGTPQASGTYVFTAQGVDYTGKVIFKKGTVVLIR"
     gene            complement(2112..>2608)
                     /locus_tag="PLY81_09135"
     CDS             complement(2112..>2608)
                     /locus_tag="PLY81_09135"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF30430.1"
                     /translation="NLNKAIAGTDYEQKTLDQINAIAGSISAAVRNNGGGHWNHSFFW
                     ESLAPNSGGLPSGNLADAINTAFGSFDEFKAKFANAGATRFGSGWAWLIVKEGKLEIC
                     STPNQDNPLMDIAETKGTPILGVDVWEHAYYLKYQNKRADYLSAIWNVVNWNKVAERY
                     NAAK"
BASE COUNT          882 a          469 c          402 g          855 t
ORIGIN      
        1 gatacagatg gagattcttt agcctataaa ttaagtgcag cgtatgatgg ccaagatgga
       61 ccaaatccta gcccaaatcc accaccacca agtgtattaa atcctgtatc attaaattat
      121 cctgcacctt ttagttcaaa taatcctttt ggcacaggtg caagcattaa cccaaaaaca
      181 ggaatcataa gtggaaaagc accacccatt gcaggtaaat atgttgtatg tgtaactgta
      241 gaagaatgga gaaatgatac tttaattaat gaacatagaa aagattttat attaagagtt
      301 gcaaactgct cttctgtaaa acctaatgca ggaccagatg atagaacttg cgatggattt
      361 acttacttct tcgaaaatca gtctcctaac cctgcaatta tttcgtatgt atggtctttt
      421 ggagatggta atacatcaac agtacctaca ccaacatata cttattccga tacaggtaaa
      481 tatttaatta aactaaaagt aaccgctact ggtggatgcc aggattcttc ttataaatat
      541 atttatgctt tccctggttt taagccaggt attaatacag ttggcacatg tgttgtttcg
      601 ccaataaata ttttagatgc cacaacaact gtttatggta ctgtaaatag ttggagatgg
      661 aattttggag atttaactac tgttgccgat acttctaata aaaaagatac tgcttggaag
      721 tatcctgccc ctggtactta caacatatct ttaattgtaa ccaatacaaa aggttgttta
      781 gataccgtaa ataaaaatgt tattgttaaa gatttaccta ccattagcct gccttttaaa
      841 gatacattaa tatgtagtat tgatacattg cccttaattg ctaatacaac tggcaacata
      901 agttgggcac ctaactataa cattattagt cctactagtt taaatccttc tgtatatcca
      961 aaagatacca caacctatgt tatcactgta aacgacaatg gttgtattag taaagattct
     1021 attaaagtaa acgtgttaga ttttatttca gtcactgcag ggccagattc aacagtatgc
     1081 ttaaccgata cttttagact gagtacaatt agccatgccc ttagttatgt ttggtctgct
     1141 tctactggag aaattgtgcc tcaagttaaa tttcctttag taaaacctac ggctattacc
     1201 aaatattatg taaccgccaa tttaggaaaa tgtcaagatt atgatacaat agtaataact
     1261 ccaattccat atcctaaagt agctataaat gccatcaatt ctatttgtta tggcgatagt
     1321 attactttaa gtacaaataa ttttattggc agtagttttt attggtctcc atcaactttt
     1381 ataactaacg caaattcact ttcgccaaca gtaaaaccta ctgatactac tttttatatt
     1441 ataactgtta acgatacatt aggttgtcca aaaccattta gcgattctat tcaagtaaat
     1501 gtaataccaa aagtggtagt gtttgcaggt aatgatactg tagttgtaaa aaatcaaccc
     1561 ttgcaattac aagctgtaac aagtccaatt cctaacaata gtacttatgt ttggtctcca
     1621 agcacaggta tcaataacac tactattttc aacccaattg ctactctaaa tatcaattca
     1681 gattctataa aatataaagt aacagtaaaa aggccagaag gctgtattgg ttcggatgaa
     1741 ttagtagtta aaatatttaa aacgcaaccc gatattttta taccaactgc ttttacccca
     1801 aataatgatg gaaaaaatga tattctaaaa cctattcctg ttggtatatc tcaaattgat
     1861 ttttttagaa tctacaatcg ttttggtcaa ttaatttatg aaacaaaaga atacttaaaa
     1921 ggttgggatg gaaatgtaaa tggcacacct caagctagcg gaacttatgt atttacagca
     1981 caaggggttg attatacagg taaggtaatt ttcaaaaaag gtacagtggt tttaataaga
     2041 tagcaattag attttactta taaaaaaaac gaggcttaca gtaggcctcg tttttttttg
     2101 caagcattaa tttattttgc agcattatat ctttctgcaa ctttattcca gttaaccaca
     2161 ttccaaatgg cacttaaata atcggcacgt ttattttgat attttaaata gtatgcatgc
     2221 tcccaaacat ctacacctaa aataggggtt ccttttgttt cagcaatatc cattaaaggg
     2281 ttatcttgat taggtgttga gcaaatctct aatttccctt ctttaacaat taaccaagcc
     2341 caaccacttc caaaacgtgt ggcaccagca ttggcaaact ttgctttaaa ctcatcaaaa
     2401 ctaccaaaag ctgtattaat agcatctgct aaattgccac ttggtaagcc accactgttt
     2461 ggtgcaaggc tctcccagaa aaaagaatgg ttccaatgac ctccaccatt atttctaact
     2521 gcagcagata ttgaaccagc aatagcattt atttgatcta atgttttttg ttcataatct
     2581 gtaccagcaa tggctttgtt taaattat
//
LOCUS       DAOQHP010000317         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_27972_length_2600_cov_5.913556, whole genome shotgun sequence.
ACCESSION   DAOQHP010000317 DAOQHP010000000
VERSION     DAOQHP010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27972_length_2600_cov_5.913556"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..721
                     /locus_tag="PLY81_09140"
     CDS             299..721
                     /locus_tag="PLY81_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNF30431.1"
                     /translation="MKAHNGFTLIELMITVAIIGVLMSIGLPLYARYQDKARIQASLY
                     EISAPKAQFEVQVNEGNTNITLADLGITSSSSANCTLITFNYDSTIPEWSLTCTLKGS
                     PLIANRTISAVRNNNGTWSCKTAGTPAIPTDLKPSNCT"
     gene            729..2141
                     /locus_tag="PLY81_09145"
     CDS             729..2141
                     /locus_tag="PLY81_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HNF30432.1"
                     /translation="MTIFVQRALEYLVLFILMLTSLIIWSGYSPFSTQSYNDWRLFEI
                     VILFILNIYYFFIPKQHINIFSTITLIIYILFFIGLISSYQSTFLSRSLRDWALYASI
                     FSSIFSLGYIIKNNRNFALYTFLALCAMPLFFIAEFIYQILFYYLSNINDKAPIGDIW
                     LGQFSHPRIFGDTALPTFFMLYAATLINQKYRLYFSILLIFLSTLIVFCGGRGLFISF
                     LLTFIFGYYLTPQIRKDILYSIFFLIIGFSINMFLSYMLSNSYQSIAFRQDSSGRSEI
                     ISYITLHIMDNLFLGISPAQFYLESVKFNIRVSHPHNLFLQILVEWGLIAFILFIILL
                     IKLLVKIIKSAAQEQNKFNYFIILGVFSFLVNCNFNGAHIYPSSQIYGLFLMAWLISI
                     YFMQEHNIKYQSFKLIHYITATVQLSVGLILVITTFMALGCGSSELATKINLAGPRFW
                     LYDSPHSDQICLDATTFFDK"
     gene            complement(2143..2493)
                     /locus_tag="PLY81_09150"
     CDS             complement(2143..2493)
                     /locus_tag="PLY81_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNF30433.1"
                     /translation="MVVEEKIMHHLDKDQLAELKEVLEDEFDVLINTYLTDAQLRLQL
                     IEQGISAQNYEQVRLAAHSLKGASANIGALQLAQLCEHLEHDCKAEDYSRLSILFSNT
                     VNEVEVVKNLLNNL"
BASE COUNT          831 a          438 c          368 g          963 t
ORIGIN      
        1 tggtacacca aaaactctta cttggactcg tactactact ggtacttgga cttgtaaaac
       61 cagtgcagct gcaaaatatg ctccaagtgg ttgtccaggt tcttaatagt taatttaact
      121 gtaagacact aaaaaagccc aatttctgtt gggctttttt atcaatgtga tggttgggaa
      181 tgttatagcc acaatttatt aaataaagaa caacatcaac gatggttctt aaacttaaaa
      241 tctgctagca aaaatttagc tagcagattt ttaaaacctc ctatagaaga ggtgtattat
      301 gaaagctcat aacggattta cattaattga attaatgatt acagtggcta ttattggagt
      361 attaatgtct atcggtttgc ctctttatgc tcgttatcaa gacaaagcac gaatacaagc
      421 aagcctctac gaaatttctg caccaaaagc tcagtttgaa gtacaagtaa atgaagggaa
      481 caccaatatt acacttgctg acttgggcat cacatctagt tcctctgcaa attgcacatt
      541 aattacgttt aattatgatt caaccattcc cgagtggtct ctcacctgta ctttaaaagg
      601 ctcaccgctt attgctaatc gcaccatcag tgcagtcaga aacaacaatg gtacatggag
      661 ttgtaaaacg gctggcacac cagcaattcc taccgatcta aaaccaagta actgcacata
      721 atttgtttat gactatattt gtacaaagag ctctagaata tcttgttctg tttatcttaa
      781 tgctaactag tttaataatt tggagtggct actccccttt ctcaactcaa tcttataatg
      841 actggcgcct atttgaaata gtaattttat ttatattaaa tatatattat ttttttattc
      901 ccaaacaaca cattaatatt ttttctacaa ttacgcttat aatatatata ttatttttta
      961 taggcttaat cagcagttat caatcaactt ttctaagcag atctttaaga gactgggcat
     1021 tatatgcaag tattttttct agtattttta gtctaggata tataattaaa aacaatagaa
     1081 actttgcttt atatactttc ttagcactat gtgctatgcc attatttttt attgctgaat
     1141 ttatatatca aatattattt tattatttat ccaatatcaa tgataaagca ccaataggcg
     1201 atatttggct agggcaattt tcccatccta gaatttttgg agatactgcg ctacctactt
     1261 tcttcatgtt atatgctgca acattaatta atcaaaaata taggttatat ttttctattt
     1321 tattaatttt tttgagtacc ttgattgttt tttgtggtgg tcgtggttta tttatttctt
     1381 ttttattaac ctttatattt ggatactatt taacacctca aattaggaaa gatattttat
     1441 atagtatatt ttttttgatc attggcttca gtattaatat gtttttaagc tatatgctat
     1501 caaatagcta tcagtctatt gccttcagac aagatagcag tgggagaagt gaaattatta
     1561 gttatataac attacacatc atggacaatt tgtttttggg tatttctcct gcccaatttt
     1621 atctagaaag tgttaaattt aatattagag tatcacatcc tcataactta tttttgcaaa
     1681 ttttagttga gtggggatta atagcattta tattatttat aatattatta ataaaattac
     1741 tagtaaaaat cattaaaagt gcagctcagg agcaaaataa atttaactat tttattattt
     1801 taggggtctt ttcttttcta gttaattgta atttcaatgg tgcacatatc tacccatcta
     1861 gtcaaatata tggcttattt ttaatggcat ggctgatctc tatttatttt atgcaagaac
     1921 ataatataaa atatcaatca tttaaactta ttcattatat aactgctact gttcaattga
     1981 gtgttggctt aattttagta attaccactt tcatggcact tggatgtgga agcagcgagc
     2041 ttgcaacaaa aatcaattta gctgggccac gcttttggct atatgactca cctcatagtg
     2101 accaaatttg tctagatgca acaacttttt ttgataaata actcaaagat tatttaatag
     2161 attcttgacc acctcaacct cattaacagt attactaaat aaaatagata atcttgaata
     2221 atcctctgcc ttacaatcat gttctaaatg ctcacatagc tgagctaatt gtaaagcgcc
     2281 gatattggca cttgcacctt ttaaactgtg tgctgctaag cgaacttgtt cataattttg
     2341 tgctgatatt ccttgttcga ttaactgtaa gcgtaattgt gcatctgtta aataggtatt
     2401 aattaataca tcaaactcat cttctaacac ttcttttaac tccgctaact gatctttatc
     2461 taaatgatgc attatttttt cctctacaac catttccaat caaactcaac gataacacta
     2521 ttaccagagc ctataaactc taatcgttga cacaatgttt caataaggcg aatgccccga
     2581 ccatgataat tcattaaatg
//
LOCUS       DAOQHP010000318         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28060_length_2596_cov_3.488784, whole genome shotgun sequence.
ACCESSION   DAOQHP010000318 DAOQHP010000000
VERSION     DAOQHP010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28060_length_2596_cov_3.488784"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="nosD"
                     /locus_tag="PLY81_09155"
     CDS             <1..897
                     /gene="nosD"
                     /locus_tag="PLY81_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNF30434.1"
                     /translation="YENKYVVIENNKLINNFFGIYLQYAKHCLVKNNTLTASQKEEYQ
                     SGNGIHCWKSDSLQIIGNRVYGHRDGIYFEFVTHTVIWRNIAHKNLRYGLHFMFSHKN
                     AYFTNFFKDNGAGVAVMFTKEVVMMNNTFQENWGDAAYGVLFKELSDCYLSGNKFIRN
                     TTGIFFEGSNRIVAEKNTFINNGWGMRLQANCIDNRIDKNNFLGNTYDISTNGFLTLN
                     HFDANYWDKYEGYDLNKDNIGDVPFRPLSLFSVIVEKNSPAMLLYRSFMISLLDKSEK
                     ILPTLTPENFIDNNPRMTPYKL"
     gene            894..1604
                     /locus_tag="PLY81_09160"
     CDS             894..1604
                     /locus_tag="PLY81_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF30435.1"
                     /translation="MIKIENITKHFGRFRALNNLSIELNKGECIALIGPNGCGKTTLI
                     KSILGMVLPELGTIKFDGKSIKNDNEYRKHIGYMPQIGRYPENMTIGQVIEMIKGIRN
                     HTNDLDTELYEKFEIEKMLNKKMRTLSGGTTQKVSAVLAFMFQSKVFILDEPTAGLDP
                     ISSEILKNKIIDAKNKGCLILITSHLLSELDDLVTQIVFMQEGKLILHENVEILKEKT
                     GEATIAKSIIHLLKTQRA"
     gene            1601..2368
                     /locus_tag="PLY81_09165"
     CDS             1601..2368
                     /locus_tag="PLY81_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF30436.1"
                     /translation="MIRVLKYVALDILKNKIIIAYTIMLALFAWSAFSLEDNESKGVL
                     TVLNIVLLTVPLVSILFSTIYIYNSSEFIELLISNPVNRGMVWKALFIGLSLSMVTAY
                     IVGVGIPLLLFANFATAIMMVATGIMLSMIFVSVAFLCSMFTRDKAKGIGVSIMLWLY
                     FALLFDGIVLFLFFQFADYPIEKIVVILSALSPVDLSRILILLKLDVSAMLGFSGAIF
                     QNYFGSNWGLVLSFALLGLWIAVPFAISLRKFKKKDL"
     gene            2396..>2596
                     /locus_tag="PLY81_09170"
     CDS             2396..>2596
                     /locus_tag="PLY81_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNF30437.1"
                     /translation="MKQDISNNSDIEKVVSVFYERVKNDDLLGHFFTGVMNVNWDKHF
                     VMMCSFWENVLFYTGDYEGDPLN"
BASE COUNT          930 a          290 c          462 g          914 t
ORIGIN      
        1 tatgaaaata aatatgttgt aattgaaaat aataaactaa tcaataattt ttttggaata
       61 tatctacaat atgcaaaaca ttgcttagta aaaaacaata cactaaccgc ctctcaaaaa
      121 gaagaatatc aatcgggtaa tggaattcat tgttggaaga gtgattcttt gcaaataatt
      181 ggtaatagag tgtatggcca cagagatgga atttattttg aatttgttac acacacagta
      241 atttggagga atattgccca taaaaattta cgatatggtt tgcattttat gttctctcat
      301 aaaaatgcct actttaccaa tttttttaaa gataatggag caggggtagc cgttatgttt
      361 actaaagaag tggtaatgat gaataacact tttcaagaaa attggggcga tgcagcttat
      421 ggagttttat ttaaagaatt atccgattgt tatttatcag gaaataaatt tattcgtaac
      481 actacaggta ttttttttga aggctcaaac agaattgttg cagaaaaaaa cacttttata
      541 aataatggtt ggggaatgcg tttgcaagca aattgtatag ataatagaat tgataagaat
      601 aactttttag gcaatacgta tgatatttct actaatggtt ttttaacgct aaatcatttt
      661 gatgcaaact attgggataa gtatgaagga tatgacttga ataaagataa tattggcgat
      721 gtgccattta ggccattgag tttattttcg gtgattgttg aaaaaaattc accagcaatg
      781 ttgctttaca gaagttttat gatatctctt ttagataagt cagaaaaaat tttacctact
      841 ttaacaccag aaaattttat tgataataac ccaagaatga caccctacaa actatgatta
      901 aaattgagaa cataacaaaa cattttggca ggtttagggc attaaataat ttaagtatag
      961 agttaaataa aggggaatgt attgcactaa ttggcccaaa tggttgcggt aaaacaacat
     1021 taataaaatc aattttagga atggttttac cagaattagg tacaataaaa tttgatggta
     1081 aaagcataaa aaacgataat gagtacagaa aacatattgg ttatatgcca caaattggtc
     1141 gttaccctga aaatatgact atagggcaag ttatagaaat gattaaaggc attagaaatc
     1201 atacaaatga tttggatact gaattgtatg aaaagtttga gatagagaaa atgttgaata
     1261 aaaaaatgag aaccttaagt ggtggtacta cacaaaaagt gagtgcagtg ttggctttta
     1321 tgtttcaatc caaagtattt attttagatg agccaactgc aggtttagat ccaatatcct
     1381 ccgaaatact aaaaaataaa attatagatg caaaaaataa aggctgctta attctcatta
     1441 catctcattt attaagcgag ttagatgatt tagttactca gatagttttt atgcaagaag
     1501 gaaaattaat attacatgaa aatgtagaaa tattaaaaga aaaaaccgga gaagctacaa
     1561 tagcaaaatc tattattcac cttttaaaaa cacaaagggc atgataagag ttttaaaata
     1621 tgttgcacta gatattttaa aaaataaaat tataattgct tatacaatta tgcttgctct
     1681 gtttgcatgg agtgctttta gtttagaaga taatgaatct aaaggtgtat taacagtgtt
     1741 aaatattgtt ttacttactg ttccattggt ttccatatta ttttctacca tttacattta
     1801 taatagtagc gagtttatag aattattaat tagcaaccct gttaatagag gcatggtttg
     1861 gaaagcttta tttattggct tatcattaag tatggtaaca gcttatattg ttggcgttgg
     1921 cattccatta ttactatttg caaattttgc aacagcaata atgatggttg caacaggcat
     1981 catgctttct atgatatttg tttctgtagc atttttatgt agtatgttta caagagataa
     2041 ggcaaaaggc ataggagttt ctatcatgct atggctgtac tttgctttgt tgtttgatgg
     2101 gatagttttg tttttatttt ttcagtttgc cgattatcct atagaaaaaa ttgtggtaat
     2161 tcttagtgct ttaagccctg tagatttaag tagaatttta attcttctta aattagatgt
     2221 atctgcaatg cttggctttt cgggagctat ttttcaaaac tattttggta gtaattgggg
     2281 cttggtttta tcctttgctt tattaggatt atggattgca gttccattcg caatttcatt
     2341 aagaaaattt aaaaagaaag atttgtaaaa ttattaattg tttaaataat aataaatgaa
     2401 acaagatata agtaataaca gcgatattga aaaagtagta agtgtttttt acgaaagagt
     2461 aaaaaatgat gatttattgg gtcatttttt tactggtgtt atgaatgtta attgggataa
     2521 acattttgta atgatgtgct ctttttggga gaatgtgctt ttttatacag gagattatga
     2581 aggagaccca ttgaat
//
LOCUS       DAOQHP010000319         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28204_length_2588_cov_3.559416, whole genome shotgun sequence.
ACCESSION   DAOQHP010000319 DAOQHP010000000
VERSION     DAOQHP010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28204_length_2588_cov_3.559416"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1984)
                     /locus_tag="PLY81_09175"
     CDS             complement(341..1984)
                     /locus_tag="PLY81_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF30438.1"
                     /translation="MKLFSTLLLSILTTLSFAQKTTSTATPNTSKPKLVIGIMIDHMR
                     WDYLYKYQNRYTNDGFKKLLKEGFTCENTFIPYTPTATACGHASAYTGTVPAIHGIAS
                     NNWWDMKQQQYVYCTDDSTVKGVGSNSKKGLMSPRNMVANTITDELRLASNFKSKVIG
                     IALKDRGSILPAGHSANAAYWYDETKGIFISSTYYMNDLPKWVKDFNAQNWVDKYYAE
                     GWNTLYPIETYTQSEGDDNNYERTNWIEVDKPVFPYKFSQHISKDYKKFSYMPQSNTY
                     TLNMARAALKAENMGKNGVTDFLAISLSAPDYMGHSFGPASVEAEDMYLRLDKDLAAF
                     IQYLDTYIGKGQYTLFLSADHGGAHIAGYLQKHKIPGGSLGLKKIVSDLNKTIAQQFG
                     ISKAIAYQTYYNISLNHAQIDSAKINLQTIKDFIINSIEKEDGVYRVIDKQKLETSGL
                     PFKIKESLINGYYPNRSGDLQVIPLPQWMRDTEKGTDHSAWNPYDAHIPLVWYGYGIK
                     QGKTNKETYMTDIAVTLAALLKIQMPNGSIGKVIEEVLK"
     gene            2285..>2588
                     /locus_tag="PLY81_09180"
     CDS             2285..>2588
                     /locus_tag="PLY81_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNF30439.1"
                     /translation="MADIFEKLIKNYGPIGQHRERAHGYFAFPKLEGPISSRMNFRGK
                     EVIVWSLNNYLGLANHPAIRKVDAEASEQWGLAYPMGARMMSGNSTHHEKLEAELAA"
BASE COUNT          850 a          390 c          424 g          924 t
ORIGIN      
        1 aagagataac agaaagaaac aacagaataa aaactaagca gcctgtttaa caacttcttc
       61 aattactgta attttaatta attacaatgt aacctcatta aataataaca aaagcaataa
      121 aaagcccttg catatatttt gcaagggctt ttatatggta tagggtatat tattaaaaga
      181 ggagtaaatt tttgtgaata ggtttatggt aagattattt atcattgtta ttcaaataaa
      241 cctaaatagt aatttataaa ttaaaatcta ccatagttga gataaataaa aaaatcatct
      301 gcattacaga tgattttacg aaagtgcaac agtataaaat ttattttaaa acttcttcaa
      361 tcactttacc aatactacca ttaggcattt gaatttttaa caatgcagca agagtaactg
      421 caatatcggt catataagtt tctttattgg ttttgccttg ttttattccg taaccatacc
      481 aaaccaatgg aatgtgtgca tcataagggt tccaggcact atggtctgta cctttttctg
      541 tatctctcat ccattgtggt aaaggaataa cttgtaaatc tccacttcgg ttaggatagt
      601 aaccattaat taaagattct ttaattttaa aaggcaagcc agatgtttct aatttttgtt
      661 tatctataac tctatatact ccatcttctt tttctataga attaataata aaatctttta
      721 tagtttgaag attaattttt gccgaatcta tttgagcatg atttaaggaa atgttatagt
      781 atgtttggta agcaatagct ttagaaatac caaattgttg agcaattgtt ttatttaggt
      841 cagaaacaat ttttttaagc cctaatgagc cgcctggtat tttatgtttt tgtaaatatc
      901 ctgcaatatg tgctccccca tgatcggcag aaagaaataa tgtatattgg ccttttccaa
      961 tataagtatc taaatattga ataaaagctg caaggtcttt gtctaatcgt aggtacatat
     1021 cttctgcttc aacagatgct gggccaaagg aatgtcccat ataatcaggg gcagataaac
     1081 taatagctaa aaaatcggtt actccgtttt tgcccatatt ttctgccttt aaagctgccc
     1141 tagccatatt taaagtatag gtattgcttt gtggcatata gctaaatttt ttatagtctt
     1201 tactaatatg ttgtgaaaat ttgtatggaa aaacaggctt atctacttct atccaattgg
     1261 ttctttcgta attgttatca tctccttcgc tttgagtgta agtttctatt gggtacaaag
     1321 tattccatcc ttcagcatag tacttatcta cccagttttg agcattaaaa tctttaaccc
     1381 atttaggtaa atcgttcatg taatatgtgc tgcttataaa aatccctttg gtttcatcgt
     1441 accaataggc agcattggca ctatggccag caggtaaaat agagcctctg tcttttaaag
     1501 caataccaat tactttactt ttaaagttag atgctaacct taattcgtct gttatagtat
     1561 tagcaaccat attccttggg ctcattaatc cttttttaga gttgcttcca acgcctttta
     1621 cagtgctatc atcggtacaa tatacatatt gttgctgttt catatcccac caattattac
     1681 ttgcaatacc atgtatagct ggtacagtgc cagtataagc actagcatgg ccacaggctg
     1741 ttgcagttgg tgtataaggt ataaaagtat tttcgcaagt aaagccttct tttaaaagct
     1801 ttttaaagcc atcattggta tatctgtttt gatatttata taaataatcc caacgcatgt
     1861 gatctatcat aatacctatt accaattttg gttttgaagt atttggtgta gccgtactgg
     1921 ttgttttttg tgcaaaggaa agagtagtta aaatagataa taggagagta ctgaataatt
     1981 tcataagtta tttattatta tttatggtaa atgccaatta tttaataaac tggtataaaa
     2041 agtatttagg ctaatagaaa aagtttaatt tatcacgttg ttatttcatg ttttattgta
     2101 gcaaatgtaa taataaactt actgatttga tttattggtg ttatttgata aacatttacc
     2161 tttgcggcaa tttttatttt gttcagcaat gtaaaaagcg tacaattgtg cgacgcaaca
     2221 ggagctaaat aatcattctc ctgccaagta aataatatgt tttttaaaat tttaacttaa
     2281 ttcaatggca gacatttttg aaaaattaat taaaaattat gggccaattg gtcagcatag
     2341 agaaagagct catggatatt ttgcttttcc taaattagaa ggtcctatta gcagtagaat
     2401 gaactttaga ggtaaagaag taatagtgtg gagtttgaat aattatttag gattagctaa
     2461 ccatcctgca attaggaaag tggatgcaga agcatcggaa caatgggggc ttgcttaccc
     2521 aatgggtgca agaatgatga gtggaaactc tacccatcat gaaaaattag aagcagagct
     2581 agcagctt
//
LOCUS       DAOQHP010000320         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28218_length_2587_cov_3.178515, whole genome shotgun sequence.
ACCESSION   DAOQHP010000320 DAOQHP010000000
VERSION     DAOQHP010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28218_length_2587_cov_3.178515"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLY81_09185"
     CDS             <1..623
                     /locus_tag="PLY81_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF30440.1"
                     /translation="IYKICNVWARVWYFFVGVKHQEIYETEHEVNKQYIFVANHISYM
                     DIPPIVTSIHQPFRALGKYEMVKVPIFGWIYRSAVILVDRSSAEKRSKSVRALKAAIQ
                     HGISVFIFPEGTFNMTEHPLKNFYDGAFRIAIETQTPIKPMLFVDSLKRLHYKSLFEL
                     TPGINRTIYLEEVSVDGLTMKNLHELKEVVYKKMEEGLKRYNNIKV"
     gene            629..>2587
                     /gene="priA"
                     /locus_tag="PLY81_09190"
     CDS             629..>2587
                     /gene="priA"
                     /locus_tag="PLY81_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNF30441.1"
                     /translation="MNNDFQNINNDNSETKKLFASVIIPLSLPETYTWEIPDQFINSV
                     KVGVRVEVQLRNKKYAGIIKSITNIVPTSFKPKHILNILDEEPLLYEKQLLLWRWIAN
                     YYMCTEGDVMQAALPANLKLTGESVLVWNEENGIDLNNYTFLSDEEYLITEALELKKE
                     LKISEVQKIIEASHVYPIIRNLIDKKVCFIWEELKEKYKPKTDTFILLHPVFQQESAL
                     EKLLNEWSKAPKQLELLLGYLHFIKTEGEVTQSKLLQKTNASAAQLKALVDKKILITE
                     KRNVNRINTLPKQVVIDFTLSDAQQKAFSNIQQQLQHHNVCLLHGVTASGKTQIYIKL
                     IEQYLQQDKQVLFMLPEIALTTQIIRRIQKHFGGYIAIYHSKFNSNERVELWNRVKTN
                     DIRIVLGARSALFLPFNNLGLIIVDEEHDSSYKQQDPAPRYHARDAAIYYASLFKNET
                     ENNTAKVVLGSATPSIETYFNAQNGKYGLVQLNERFANVDMPTIQLIDLKKVIANEQI
                     NQNSKNEQLKIALSPELLNAMAISLKNKKQIILFQNRRGYSPYMICNACGWIPQCKHC
                     DVTLTFHKAKNKLSCHYCGTSYPVLNSCVVCGSHHFTNKNFGTEKIEEILAEHFTDAK
                     IARMDIDSIKGKNAHDNLIKLFEQQRIDI"
BASE COUNT         1003 a          342 c          424 g          818 t
ORIGIN      
        1 taatttataa aatttgtaat gtatgggcaa gggtatggta tttttttgta ggtgtaaaac
       61 atcaagagat ttatgaaacg gagcatgaag taaataagca atatattttt gttgctaacc
      121 atatttctta tatggatatt ccacccattg taacttctat tcatcagcca tttagggctt
      181 tagggaaata tgaaatggtt aaagtaccca tatttggttg gatttacaga tctgctgtta
      241 ttttggtgga tagaagtagt gcagagaaaa gatctaaaag tgtaagagca ttaaaggcag
      301 ctattcaaca tggtatttca gtttttattt ttcctgaagg aacttttaat atgacagagc
      361 atcctctaaa aaatttttat gatggtgctt ttagaattgc aattgaaacc caaactccca
      421 taaagcctat gttatttgta gattcgctaa aaagattaca ttataaaagc ttatttgaat
      481 taacacctgg tattaacaga acaatttatt tagaagaagt ttcagtagat gggttaacta
      541 tgaaaaattt gcatgagcta aaagaggtag tgtataaaaa aatggaagaa ggattaaaga
      601 gatataacaa tattaaagtc taaaaaattt gaacaacgat tttcaaaata ttaataatga
      661 caattcggaa acaaaaaaac tatttgcttc tgttattatt cctttatcct taccagaaac
      721 atatacctgg gaaatacctg atcaatttat taattctgta aaagtaggcg ttagggtaga
      781 ggtacaatta agaaataaaa aatatgctgg cattataaaa tctattacca acattgttcc
      841 tacaagtttt aaaccaaagc atattttaaa tatattagac gaagagccat tgttgtacga
      901 aaagcaatta ttgctttggc gttggattgc caattattat atgtgtacag aaggagacgt
      961 tatgcaagct gcattacctg ctaatttaaa gttaacagga gaaagtgttt tagtttggaa
     1021 tgaggagaat ggtattgatt taaataacta caccttttta agtgatgaag aatatttgat
     1081 aacagaagcc ttagagctaa aaaaagaact taaaataagt gaagtacaaa aaattattga
     1141 agcaagtcat gtttatccaa ttatcagaaa cttgatagat aaaaaagtat gttttatttg
     1201 ggaagagttg aaagaaaaat ataagcctaa aacagataca tttattttgt tacaccctgt
     1261 ttttcaacaa gagagtgcat tagaaaaatt attgaatgaa tggagtaaag cacccaaaca
     1321 attagaactt ttattaggct atttgcattt tataaaaaca gaaggagaag ttacacaatc
     1381 taaactctta caaaaaacaa atgcatctgc tgctcaatta aaagcattag tagataaaaa
     1441 aattttaata acagaaaaga gaaatgttaa tagaataaat actttaccga agcaagtggt
     1501 tattgatttt actttatctg atgctcagca aaaagcattt tccaatattc agcaacaact
     1561 acaacatcat aatgtttgct tattacatgg tgttactgca agtggtaaaa cacaaattta
     1621 tataaaacta attgagcaat atttacaaca agataaacag gttttgttta tgctgccaga
     1681 aattgctttg accacccaaa ttattagaag aattcaaaaa cactttggtg gttacattgc
     1741 aatttatcat tctaagttta actctaatga aagggttgaa ctttggaata gagtaaaaac
     1801 aaacgatatt agaattgtac ttggagcaag aagtgctctg tttttaccat ttaataattt
     1861 aggattaata attgttgatg aagaacatga ttctagctat aaacaacaag acccagcacc
     1921 tcgttatcat gcaagagatg ctgcaatata ttatgcttct ttatttaaaa acgaaacaga
     1981 aaataataca gctaaagttg tattaggtag tgcaacacca tccatagaaa cttattttaa
     2041 tgcacaaaat ggaaaatatg gtttggtaca attaaatgaa cgatttgcaa atgtagatat
     2101 gccaaccatt caattaattg acttaaaaaa ggtaattgcc aacgaacaaa tcaatcaaaa
     2161 ctctaaaaat gaacaactga aaattgcttt atcgccagag ttattaaatg caatggctat
     2221 ttcattaaaa aataaaaagc agataattct ttttcaaaat agaagaggct acagcccgta
     2281 tatgatttgc aatgcttgtg gttggattcc tcaatgcaag cattgcgatg ttacattaac
     2341 ttttcataaa gctaaaaaca aattaagttg ccattattgt ggtacttctt accctgtatt
     2401 aaactcctgt gtggtgtgtg gtagtcatca ttttaccaat aaaaattttg gtaccgaaaa
     2461 aattgaagaa attttagcag aacattttac cgatgcaaaa attgccagaa tggatataga
     2521 cagcattaaa ggcaaaaatg ctcatgataa cttaattaaa ctttttgaac aacagcgaat
     2581 agatatt
//
LOCUS       DAOQHP010000321         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28502_length_2572_cov_3.596345, whole genome shotgun sequence.
ACCESSION   DAOQHP010000321 DAOQHP010000000
VERSION     DAOQHP010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28502_length_2572_cov_3.596345"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLY81_09195"
     CDS             <1..1262
                     /locus_tag="PLY81_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF30442.1"
                     /translation="FGEMGGYTAESDAAALLGSLGVKEEFHNSLMKDIPGNLKVRVLL
                     AQALFGNPDILLLDEPTNGLDIETIGWLENFLADYENIVLVVSHDRHFLDAVCTHVAD
                     VDRQKIKTFTGNYTFWYESSQLMSRQINDKNKKVEEKRQALIDFIARFSANASKSKQA
                     TSRKKALEKLTIEEIEPSNRKYPGIIFQPLREVGNQILNVENLSKTIDGRTLFSKATF
                     SVNKNDKIAFLSKDPLAVTNFFEIINDNIKSNEGKFEWGTTITKAYLPMENNEFFTEG
                     LSLMEWLRQFVPAHVTDADEPFLRGFLGKMLFSGDDIAKKTNVLSGGEKVRCMISRMM
                     LQNPNLVVLDQPTNHLDLESIQSFNEGCQTFPGIVLLTSHDHTFIQTVANRIIELTPK
                     GIIDRLMTFDEYMDDKRVQELRIEMYS"
     gene            complement(1455..2572)
                     /locus_tag="PLY81_09200"
                     /pseudo
     CDS             complement(1455..2572)
                     /locus_tag="PLY81_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
BASE COUNT          920 a          410 c          404 g          838 t
ORIGIN      
        1 agtttggaga aatgggaggc tacactgccg aaagtgatgc agcagcttta ttaggcagct
       61 taggtgttaa agaagagttt cataatagct taatgaaaga cataccaggc aatttaaaag
      121 ttcgtgtatt acttgcacaa gcactatttg gcaatccaga tattttatta ttagatgagc
      181 ctaccaatgg attagatatt gaaacaattg gttggcttga gaatttttta gccgattatg
      241 aaaatatagt tttagtagta agccatgaca ggcacttttt agatgcagtt tgtacacatg
      301 ttgctgatgt ggacagacaa aaaataaaaa cctttacagg taactataca ttttggtatg
      361 agtcctctca attaatgtct cgtcaaataa atgataaaaa caaaaaagtt gaagaaaaac
      421 gccaggcctt gattgatttc attgcacgtt tctctgccaa tgcatctaaa agtaaacaag
      481 ctacttctcg taaaaaagct ttagaaaaat taactataga ggaaattgaa ccaagtaata
      541 gaaaatatcc gggaattatt tttcaacctt taagagaggt aggtaatcaa attttaaatg
      601 tagaaaattt aagcaaaacc atagatggaa gaaccttatt tagcaaagca acttttagtg
      661 taaataaaaa tgataaaatt gcctttttaa gtaaagaccc cttagctgtt actaactttt
      721 ttgaaataat aaacgataac attaaaagta atgaaggtaa gtttgaatgg ggaactacca
      781 ttacaaaagc atacttacct atggaaaata atgaattttt tacagagggt ttaagtttaa
      841 tggaatggct tcgtcaattt gtaccagcac atgtaacaga tgcagatgaa ccttttttaa
      901 gaggctttct aggaaaaatg ttatttagtg gagatgatat tgctaaaaaa acaaacgtat
      961 taagtggagg agaaaaagtg cgttgtatga ttagcagaat gatgcttcaa aaccctaatt
     1021 tggttgtatt agaccaacca acaaaccatt tagatttaga aagtattcaa agctttaacg
     1081 aaggttgcca aacttttcca ggaattgtat tattaaccag ccacgaccac acttttatac
     1141 aaacagttgc caatcgtatt attgagttaa cacctaaagg aattatagac agattaatga
     1201 cttttgatga atatatggat gataaaagag tgcaggaatt aagaattgaa atgtatagtt
     1261 aaataacata attacattaa aataatactt taaagaggtt gcttaaaaca ataagcagcc
     1321 tctttaactt tttataaggc tcctaattat ttttgttttt ttcataggta agttagaagc
     1381 taagcaattt ccttcaaata aaaaaggttg taatttgtat tacaacctaa ataatatttt
     1441 attgaaactt agcactattc aatgttaaaa ttcattttaa tatcgctctt attatctttt
     1501 aataatgcta tatatttttt tgcaatttca gcatcaacaa cttttccatt aattgtaatt
     1561 ataccattct catagcttaa actagctttg ctttgtagta agccgtcctt ttgtaaggct
     1621 cccacaaaat tagtatagtt gttttcagaa atggatgtgg tggttgttat ttcaatcttt
     1681 ttatcaactg taactgtttc attggaatga agttgagcca aggtaggtgc aattaccaat
     1741 gatacaatac tcattagttt aattaaaata ttcattgatg ggccagaagt atctttaaat
     1801 ggatcaccaa ctgtatctcc tgtaacagat gctttatgag gttctgattt tttgtaaaac
     1861 atttcaccat taatatttac tcctttttca aaagattttt ttgcattatc ccatgcacca
     1921 ccagcattat tttgaaacat tcctaacaat acaccactta ctgtagcacc tgctaaaaat
     1981 ccacctaaag cttcggagcc taaagtaaaa ccaataatta ttggagatag aattgcaata
     2041 gcaccaggta acatcatttt tttaatggat gcttcagtag atattgctac acatttatca
     2101 tattcaggtt ttccttttcc ttccataata ccaggaatgg ttttaaattg tcttcttact
     2161 tcttcaacca ttgccattgc tgcttctcca acagctctaa tagccaaaga agaaaagata
     2221 aaaggaatca tagctcctac aaataaagta gctaatactt ttgctttata aatatcaata
     2281 ccagtaattt tagatacccc aacaaatgca gcaaataatg ccaatgatgt taaagcagca
     2341 gatgcaatag caaatccttt gccaatagca gcagttgtat ttccagcaga atccaatata
     2401 tcagttcttt atcgaactta tttttctaat taagccattt aggcaatacc tccagcattg
     2461 tcactaattg gtccatatcc atcaattgct aagcaaatag gcaaattaga aagcattcct
     2521 agtgcagcta aggctacacc ataatatcct aatgaagtat gagcataaaa ac
//
LOCUS       DAOQHP010000322         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28593_length_2567_cov_4.410430, whole genome shotgun sequence.
ACCESSION   DAOQHP010000322 DAOQHP010000000
VERSION     DAOQHP010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28593_length_2567_cov_4.410430"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLY81_09205"
     CDS             complement(<1..249)
                     /locus_tag="PLY81_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNF30443.1"
                     /translation="MKKIGTIIKYVFFLSLGLLLLWLSFHNIRKNPEQWMQFKQTIKS
                     ANYLLFLPVFFIITASHAVRALRWRVLIEPMGYKVSFLN"
     gene            complement(366..926)
                     /locus_tag="PLY81_09210"
     CDS             complement(366..926)
                     /locus_tag="PLY81_09210"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNF30444.1"
                     /translation="MAMKIYTKTGDAGKTSLIGGTKVPKSHIRIESYGTIDELNSYIG
                     LVADYSSEEHIKIILKEIQDRLFTIGSSLATDPDKDPIMKLPDLKEEDILLLEQEIDN
                     MNKELPVMKSFILPGGHVVVSTTHIARCVCRRAERICVAMQQEEMFISPIVIKYINRL
                     SDYLFMLARYTAQQLNVQEIPWKARV"
     gene            complement(953..1654)
                     /locus_tag="PLY81_09215"
     CDS             complement(953..1654)
                     /locus_tag="PLY81_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF30445.1"
                     /translation="MNNSIIRLEDIFKNYYMGSQAITVLKGVSLEILKNEYVALMGPS
                     GSGKSTLMNILGCLDTATSGKYILNGNDVSKMTDDELAEIRNNEIGFVFQQFNLLPRL
                     SAAENVALPLVYAGVGKKERNERAMEALKMVGLEDRSHHKSNELSGGQIQRVAIARAL
                     VNNPALLLADEPTGNLDTKTSVEVMEIFGKIQQAGNTVVLVTHEEDIANYAKRVVKLR
                     DGLVETDILKEVAVM"
     gene            complement(1684..>2567)
                     /locus_tag="PLY81_09220"
     CDS             complement(1684..>2567)
                     /locus_tag="PLY81_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF30446.1"
                     /translation="RNRVGLKDPKKPIGSFIFLGPTGVGKTELARSLARYMFDSEDAL
                     IRVDMSEYMEKFTVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEKAHPDIY
                     NILLQVLDDGVLTDGAGRKIDFKNTLIIMTSNIGVRQLKEFGDGVGFATASRIQNQEE
                     NNKAVIEKALKKTFSPEFLNRIDDVIIFNSLSKENIFSIIDILMKGVTKRLGNLGFTL
                     ELTNEAKDFIAEKGYDVQYGARPLHRAIQKYLEDPLADEILNTKVQAGDILIAGFDKE
                     NNKIVFEFKSREEKEKV"
BASE COUNT          810 a          484 c          307 g          966 t
ORIGIN      
        1 atttaaaaaa gaaactttat aacccatcgg ctctattaaa actcgccaac gcaaagctct
       61 tactgcatga ctagcagtga tgataaaaaa cacaggaaga aataataaat aattggcaga
      121 tttaattgtt tgtttaaact gcatccattg ttcaggattc tttctgatat tatgaaaact
      181 taaccacaat aaaagcaagc ctaacgataa gaaaaataca tattttatta tggtgcctat
      241 tttcttcata tactacaaaa ttgcaattat tgattgatag ttgcagtaaa tttatacttt
      301 aataatttta ttttttacac cgtatatagt aatgaagaaa attataatga gtatgctact
      361 acaatttata ccctagcttt ccaaggaatt tcttgaacat ttaattgttg tgctgtataa
      421 cgtgcaagca taaataaata atcgctaagc ctattaatgt acttaattac aattgggcta
      481 ataaacattt cttcttgttg cattgccaca caaatacgtt ctgcccttct acacacacat
      541 ctagcaatat gtgtagttga aacaacaaca tgcccaccag gtaaaataaa acttttcata
      601 acaggtaatt ctttattcat attatcaatc tcctgttcta acaataaaat atcttcttct
      661 tttaaatcgg gtaatttcat tattggatct ttatcggggt ctgttgcaag cgaagaacca
      721 attgtaaaaa gtctatcttg aatttctttt aaaattattt taatatgctc ctctgatgaa
      781 taatcagcaa ctaatccaat ataagaattt aattcatcaa ttgtaccgta gctttcaatt
      841 ctaatatggc ttttaggaac ttttgttcca cctattaaag aagtcttgcc agcatcgcca
      901 gttttagtat aaattttcat tgccataaat cataattttt agaagttgct tattacatta
      961 ctgctacttc tttcaatata tctgtttcta ctaatccatc tcttaattta actacacgtt
     1021 ttgcataatt ggcaatatct tcctcgtgtg taaccaatac tacagtatta cctgcttgct
     1081 gaattttacc aaaaatttcc attacttcta ccgatgtttt agtatctaaa ttacctgttg
     1141 gctcatctgc taataaaagt gctgggttgt ttaccaaggc tctggcaata gcaacacgtt
     1201 gtatttgtcc accacttaat tcattgcttt tatggtggct tctatcttct aaacctacca
     1261 tttttaaggc ttccattgca cgttcatttc tttctttttt tcctacacct gcatatacta
     1321 atggaagagc aacattttct gcagctgata atcttggtaa taaattaaac tgttgaaaaa
     1381 caaaacctat ctcgttattt cttatttcgg ctaattcatc atcggtcatt ttacttacat
     1441 cgtttccgtt taaaatgtat ttgccactgg tggcagtatc taaacaacct aaaatattca
     1501 ttaatgtact tttaccacta ccacttggtc ccattaaggc tacatattca tttttaagaa
     1561 tttctaaaga aacaccctta agcactgtaa ttgcctggct acccatataa taattcttaa
     1621 aaatatcttc tagcctaata attgaattat tcaaaacaaa ataaatttct aatgttatat
     1681 aaattatact ttttcttttt cttctcttga tttgaattca aaaacaattt tattattttc
     1741 tttatcaaat ccagcaatta atatatcgcc agcttgcact ttggtattta atatttcatc
     1801 tgctaatggg tcttctaaat atttctgaat tgccctgtgt aaaggccttg caccatattg
     1861 tacatcatac cctttttctg ctataaagtc tttagcttca tttgttagtt ctaatgtaaa
     1921 gcctaaattg cccaagcgtt tagtaacccc tttcattaaa atatcaataa tactaaatat
     1981 gttttcttta gatagggaat tgaaaattat tacatcatca attctattta aaaactctgg
     2041 agaaaaggtt ttctttaagg ctttttctat tactgcttta ttgttttctt cttgattttg
     2101 tattcttgaa gcagtagcaa aaccaacacc atctccaaat tcttttaact gtctaacacc
     2161 aatattggag gtcatgatga ttagtgtatt tttaaaatca atttttctac cagcaccatc
     2221 tgttaaaaca ccatcatcta aaacctgtag taagatatta taaatatctg gatgtgcttt
     2281 ttcaatttca tctaatagaa taacactata aggttttctt ctcacttttt ctgttaattg
     2341 tccaccttct tcgtacccaa catagcctgg aggagcacca attaaacgac ttacagtaaa
     2401 tttctccata tattcactca tatctaccct tattaaggca tcttcactgt caaacatata
     2461 ccttgcaaga cttctggcta attctgtttt gcctacacct gttggtccta aaaatataaa
     2521 tgagccaatt ggttttttag ggtcttttaa ccctacccta tttcttt
//
LOCUS       DAOQHP010000323         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28702_length_2561_cov_3.569832, whole genome shotgun sequence.
ACCESSION   DAOQHP010000323 DAOQHP010000000
VERSION     DAOQHP010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28702_length_2561_cov_3.569832"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLY81_09225"
     CDS             <1..439
                     /locus_tag="PLY81_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNF30447.1"
                     /translation="KLSENGMKNNDGGPFGCVIVKDGKIIGRGWNKVTGTNDPTAHAE
                     VTAIRDACKNLGTFQLDGCEIYTSCEPCPMCMGAIYWARPSKVFYANTRQDAANIGFD
                     DSMIYHEITLPLTERKIPIEPLGREFALEIFNEWSNKLDKTKY"
     gene            519..1340
                     /locus_tag="PLY81_09230"
     CDS             519..1340
                     /locus_tag="PLY81_09230"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNF30448.1"
                     /translation="MSNLMQQLNETVNYIKSKVNAQATTAIVLGSGLGNLASVIKTDV
                     AIPYTDIPHFPVSTVKGHGGKLIFGELGGTKVIVMSGRFHFYEGYSAQQVVYPVRVMK
                     MLGVENLLLSNAAGAVNPNYKVGDLMLINDHISFFTTNPLIGRNEEELGTRFPDMSEP
                     YNKAILQKAKSIATANNILVHEGVYTAVTGPTFETHAEYRLIKISGSDVVGMSTVQEN
                     IAAVHCGMKVFAVSVVTDLGIREENNVITHEEVLQAAKEAEPKLTLLFTELVKVL"
     gene            1435..2523
                     /locus_tag="PLY81_09235"
     CDS             1435..2523
                     /locus_tag="PLY81_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HNF30449.1"
                     /translation="MHNKQVNLLKIFYKEWLTPLIILLCIYLISFFPTVIEKYYSNGI
                     YLYISYILRSISGSVSFSIGDVLYIILVGYLLVQGIKFFAILFSKERRKDFFKYILQT
                     AKKALWLIIFFQILWGMNYYRIGVTHQLNIPKNDYTKEDVESLVCDLVQKLNTIRPAI
                     GRNELPQPSFENIIEESILLHDSLRMKNNLLNYKKASVKKSIFSKYGHYIGFVGYYNP
                     FTAEAQLSVDVPKVLAPYIVCHEIAHQLGYAAEDEANFVGYLSCSQSNNPYFKYSVYL
                     DLYRYAAIELLMMNETNTHKWELDTLVQNDLKDIRKFFLAKANNISPTFSAIYNQYLK
                     ANRQSKGLSSYNNVIGLLISKKKKYGNI"
BASE COUNT          946 a          329 c          446 g          840 t
ORIGIN      
        1 taaactttct gaaaatggta tgaagaataa tgatggtggt ccttttggtt gtgttattgt
       61 gaaggatgga aaaataattg gacgaggttg gaataaagta acaggaacca atgacccaac
      121 tgcacatgct gaggttactg ctattagaga tgcatgtaaa aacttaggaa cttttcagtt
      181 agatggctgc gaaatttata cttcttgcga accatgtccc atgtgtatgg gggcaattta
      241 ttgggcaaga ccatctaaag tcttttatgc taatacaagg caagatgctg ctaatattgg
      301 attcgacgac tcaatgattt accatgaaat aactttacca cttaccgaaa gaaaaattcc
      361 tattgagcca ttaggcagag aatttgcctt agaaattttt aatgaatgga gtaataaact
      421 tgataaaaca aagtattaag aaaaattagt taaacatata cacaattctt gtacctaatt
      481 agcacaagta tatatttgca gacttaataa tattttatat gagcaattta atgcaacaat
      541 taaatgaaac tgttaattac ataaaaagta aagtaaatgc acaagcaact actgcaattg
      601 ttttaggaag cggtttaggt aatttagctt ctgtaataaa aacagatgta gcaattccat
      661 ataccgacat tcctcacttt cctgttagca cagtaaaagg gcatggcggt aaacttattt
      721 ttggagaact tggaggcacc aaagtaattg ttatgagtgg tagatttcat ttttacgaag
      781 gctactctgc acaacaagta gtgtacccag ttagggtgat gaagatgtta ggcgttgaaa
      841 atttattatt atcaaatgct gctggtgcag ttaacccaaa ttataaagta ggagatttaa
      901 tgttaattaa cgatcacatt agttttttta caaccaatcc gttaattgga agaaatgaag
      961 aagagttggg tactcgtttt ccagatatga gtgagcctta caataaggca attctacaaa
     1021 aagcaaaatc cattgctact gcaaataata ttttagtaca cgagggtgtt tacactgcag
     1081 taactggccc aacctttgaa actcatgcag agtatagatt aattaaaatt agtggaagcg
     1141 atgtggttgg catgagtact gtacaagaaa acatagcagc ggtacattgc ggaatgaaag
     1201 tttttgcagt aagtgtagta acagatttag gtattagaga agaaaacaat gtaattacac
     1261 acgaagaagt tttacaagca gcaaaagaag cagagccaaa attaactttg ctatttacag
     1321 aattagtaaa ggttttataa ccaattaata ttttttgaaa tccgattatg aaaataatcg
     1381 gatttttttt ataattgaat cttaattgta aataattata gcaaattttt taaagtgcat
     1441 aataagcaag ttaatttatt aaaaatattt tataaagaat ggcttacacc tttaattatt
     1501 ttattgtgca tctacctaat tagtttcttc cccactgtaa ttgaaaagta ttatagcaat
     1561 ggaatttatc tatacataag ttatatttta cgtagtattt caggtagtgt aagttttagc
     1621 attggtgatg ttttatatat aattttagta ggttatttat tagtacaggg tattaaattt
     1681 tttgcaatac tattttcaaa agaaagaaga aaagattttt ttaaatacat tttacaaaca
     1741 gctaaaaaag ctctttggct aattattttt tttcaaattc tttggggaat gaattattat
     1801 agaattggcg ttacccatca actaaacata ccaaaaaatg attacactaa agaagatgta
     1861 gaaagcctgg tttgtgattt agttcaaaaa ttaaatacta tacgaccagc aattggcaga
     1921 aatgaattac cacaaccttc tttcgaaaat ataatagaag aaagtatttt actacacgat
     1981 agcttaagaa tgaaaaataa tttacttaac tataaaaaag cttctgttaa aaaaagtata
     2041 ttcagcaaat atggacatta tatagggttt gtaggatatt ataatccttt tactgccgag
     2101 gctcaattaa gtgtagatgt acccaaagta ttagcacctt atattgtttg ccacgaaatt
     2161 gctcaccaat taggatatgc agcagaggat gaagcaaatt ttgtaggtta tttatcttgt
     2221 agccaaagta acaatccgta ttttaagtac tcggtttatt tagatttata taggtatgca
     2281 gcaattgagt tattaatgat gaatgaaacc aatacccata aatgggagct ggataccttg
     2341 gttcaaaatg atttaaaaga tatacgaaaa ttttttctgg caaaagcaaa taatatatct
     2401 ccaacctttt cagctattta taaccaatat ttaaaagcca ataggcaaag caaagggtta
     2461 agtagttata ataatgtaat agggttatta attagtaaaa agaaaaagta tggtaatatt
     2521 tagaatagat tattatttta tttggttaga tgtaaataat t
//
LOCUS       DAOQHP010000324         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_28831_length_2555_cov_4.084400, whole genome shotgun sequence.
ACCESSION   DAOQHP010000324 DAOQHP010000000
VERSION     DAOQHP010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28831_length_2555_cov_4.084400"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..827)
                     /locus_tag="PLY81_09240"
     CDS             complement(102..827)
                     /locus_tag="PLY81_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNF30450.1"
                     /translation="MTELEKNIIAIKRRIEVACINANRNTNSVKLLLATKTVNANKII
                     EAINLGETLIGENKIQEVKEKFEALKNIPYQKHFIGYLQSNKIKDILKYEVNCIQSLD
                     RLSLAEKLHKRLTLEEKKLDVLIQVNTSFEASKFGIAPNNTIDFIKQVANFETLQIKG
                     LMTIGLFSSDADKVRQCFKNLKALQQQIIALNLPNVSMQELSMGMSGDLEIAIEEGAT
                     IIRVGTAIFGKRIYPDSYYWNEG"
     gene            1137..1757
                     /locus_tag="PLY81_09245"
     CDS             1137..1757
                     /locus_tag="PLY81_09245"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNF30451.1"
                     /translation="MFLFVITMQAQHLKPLESENDNYRYKEEKENVAIQPKYEHVGNF
                     YEGFANVLLNKKWGFIDKTGKVVIPIKYDKVDIFSEGLAPVSLNKKWGFIDKTDKVVI
                     SLKYDDANIFSEGLAAVEINEKHGFINQKGKEVISIKYDWADDFSEGFAVVELNGKFG
                     FIDKKGKVVIPFLYESAFPFKDGKAKVEKDGIEFYIDNKGNEIIEE"
     gene            complement(1860..>2555)
                     /locus_tag="PLY81_09250"
     CDS             complement(1860..>2555)
                     /locus_tag="PLY81_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30452.1"
                     /translation="KKNKKANFATIMGFLGGVLIWTGWVEFSFMWIAEKNNVANLMVN
                     GQIATKREYLVMLSSLGLLGTIMTYFFFTRSNCTFFIWFQNKLGYKNDIAPEGRYFKK
                     PLAITTFIETIMLLWFFYIVLLVVYDDSIAGDKHIATYIVAWGSLIWSIYLISKLITI
                     QVFDYAVRYAVPTVIIFWNFIEVLGRWDTFKEVWIYPIEHWFEVSLMIVLVIGLLFLF
                     LKTPYFKKELRRV"
BASE COUNT          956 a          371 c          349 g          879 t
ORIGIN      
        1 tatattaaat actaattatt atgttattac taagtgtttt tagattttta aaaaagatgt
       61 aaaaaatatt taaataaaat agtttcaatc taaatataca tttacccttc gttccaataa
      121 taactatctg ggtatattct tttaccaaaa atagcagtac caactcttat aatggttgca
      181 ccttcttcaa tagcaatttc aaggtcgcca ctcattccca ttgataattc ttgcatagat
      241 acattgggta agtttaatgc aataatttgc tgctgtaatg ctttaagatt tttaaaacat
      301 tgccgtactt tatctgcatc tgaactaaat aagccaatag tcattaaacc ttttatttgt
      361 aaagtttcaa agttggcaac ctgcttaata aaatctattg tattattagg tgcaatgcca
      421 aatttactag cttcaaaaga ggtgtttact tgaattaaaa cgtctaattt tttttcttct
      481 aaagttaagc gtttatgaag tttttcggca aggcttagcc tgtctaaaga ttgaatacaa
      541 ttaacctcat attttaaaat gtcttttatt ttattgcttt gtaaatagcc aataaaatgt
      601 ttttgatatg gaatattttt taacgcttca aatttttctt ttacttcttg tattttattt
      661 tcacctatta aagtttcgcc taaattaata gcttcaatta ttttgtttgc attaaccgtt
      721 ttggtggcaa gtaataattt tacactattt gtatttctat ttgcatttat acaggcaact
      781 tctattctgc gtttaattgc aataatattt ttctctaact ctgtcatata ttgttggtgt
      841 aaagtttaaa ataaaattaa acaccaaagc aattttgttt atgccatatt ctaaaaacaa
      901 aatccaacaa aaaattatta caacagtatt gttggatgat gttatgtgta aattggaatt
      961 atttagataa gaccttctta actctttttt aaagtacggt gttttaagga agaaaaataa
     1021 taacaatttc aactgtgata caaaaatacc ttacaaaggg tattgataaa aatagtaata
     1081 aataataaat ttacagaaaa accacattta tgaaagctgt cattaccttc tgcattatgt
     1141 ttttatttgt aataacaatg caggcacaac atctaaagcc gttagaatca gaaaacgata
     1201 actatagata caaagaggag aaagaaaatg tagcaataca gcccaaatac gagcatgttg
     1261 gtaattttta tgaaggcttt gcaaatgttt tactcaataa aaaatggggt tttattgaca
     1321 aaactggtaa agtagttatt ccaattaaat atgataaggt ggacatattt tctgaagggt
     1381 tggcaccagt cagtctcaat aaaaaatggg gttttattga caaaacagat aaagtagtaa
     1441 tatctcttaa atatgatgat gctaacattt tttctgaagg tcttgcagca gttgagatta
     1501 acgaaaaaca tggctttata aaccaaaaag gtaaagaagt aatatctatt aaatatgatt
     1561 gggcagacga tttttccgaa ggttttgcag tagttgaact caatggaaaa tttggattta
     1621 ttgacaaaaa aggtaaagta gttattccat ttttgtatga atctgccttt ccgttcaaag
     1681 acggcaaagc caaagtagaa aaagacggaa tagaatttta tattgacaac aaaggaaatg
     1741 aaattataga agaataaaca agtaaaagca tagcttccaa atcataaaat tggtaaagag
     1801 gtaaataaaa ctaacataaa ttttataaac aaactcttta ccaataatgg ttatattatt
     1861 taaactcttc ttaactcttt tttaaagtaa ggtgttttaa gaaaaagaaa taataagcca
     1921 attactagca caatcattaa agaaacttca aaccaatgct caatagggta aatccaaact
     1981 tctttaaaag tgtcccatct gcctaatact tctataaaat tccaaaaaat aataactgtt
     2041 ggcactgcat atcttacagc ataatcaaaa acttgaatgg taattaactt agaaattaga
     2101 taaatagacc aaattaagga gccccatgca acaatataag ttgcaatatg tttatctcca
     2161 gcaatactat catcataaac aactaataat acaatataaa aaaaccagag tagcataatg
     2221 gtttctataa aagttgtaat ggctaaaggt tttttaaaat accttccctc tggggcaata
     2281 tcatttttat atcccaactt gttttgaaac caaataaaaa atgtacaatt acttcttgta
     2341 aagaaaaagt aagtcataat ggtacctaat aaccctaaag aggatagcat taccaagtat
     2401 tctcttttgg tggcaatttg tccattaacc attaagttgg caacattatt tttttcggca
     2461 atccacataa aagaaaactc aacccagcct gtccatatta acacaccacc taaaaacccc
     2521 attatggttg caaagtttgc ttttttattt ttttt
//
LOCUS       DAOQHP010000325         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_29153_length_2539_cov_2.661031, whole genome shotgun sequence.
ACCESSION   DAOQHP010000325 DAOQHP010000000
VERSION     DAOQHP010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29153_length_2539_cov_2.661031"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..2120)
                     /locus_tag="PLY81_09255"
     CDS             complement(207..2120)
                     /locus_tag="PLY81_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30453.1"
                     /translation="MQKLTFSVLLFLAFSFKLFAQVDDRKDVSLKFNEYVLNNFQEKI
                     FVHTDRNFYLSGDIVWFKIYNTSASQNTFTTISKIAYVELLNTENKSLLQAKIALTDG
                     TGNGQFVLPLSLPTGNYIFRAYTNWMKNYNEEIMFEKTISVINAFEKNSIDTAKKAIS
                     YDVQFFPEGGNLVNNIQSKIAFKVINSNGFGVMTKGKIVNQNNETIQEFNANHLGIGS
                     FLFTPVNTNTYKALVFTPTNDTLSYNLPKILNDGYVMQVTRNSTHTTITVFASNYLNN
                     KAVKLVIHNNNTIQYNQTQSINNQQAVFTVHNNLLKEGINHLTIFNDYDLPVTERLLY
                     KRPTEKATLKTNINNTVFNNREKVNIMVNGVTNTNQPIETNLSVAVFKTDSFQLNNDV
                     DIQQYLWLTSELKGNIEEPAYYFNTINEETDSALDNLILTQGWRRFNWLNILQSPTTP
                     KYLPEYENHIITGKVIDKRTLQPAEGIGIYLTIPGKEFGFAAAQTSSDGSFFTLMPTI
                     YNSSELIAQTNNRTDSNYRIDINSAYWEKTTGYKPTKFLISKNNERLLNNYGISVQAN
                     YIYHSKPLYKETQAYSPFYGKADEVYFLDNYTRFSTMEEVMREYVTGVSVRKRAGKFI
                     FNLRWPRSYSNSC"
     gene            complement(2162..>2539)
                     /locus_tag="PLY81_09260"
     CDS             complement(2162..>2539)
                     /locus_tag="PLY81_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNF30454.1"
                     /translation="EYWQQLKQSSELGGSVFDVQPTQLLGNLHCVTNPNEPIIGYVSA
                     TTTAEKRIYILNTNVPTFVQPYPATCEFAVVANNKDSMKVYYTNKRYIPIAPDMQGAY
                     LAGSTQCTDCRVLGGTTIKPAFW"
BASE COUNT          765 a          381 c          378 g         1015 t
ORIGIN      
        1 taaagaagtt aaaagaggct ccttaataaa ctgttgattg cactgtcttc ttttgttgag
       61 ctctctttac tattctttat tctatagttc cttttgtgac taattgataa acgttaacat
      121 tctggctttg acctattcta tgagctctgt ctgttgcttg agcatccata gttggattcc
      181 aatcattatc ataaaatata actgtgttag cagctgttag agtaactccg aggccacctt
      241 aaattaaaaa taaatttacc tgctcttttt cgtacagata cgcctgtaac atattctctc
      301 attacctctt ccattgtaga aaatctggtg tagttatcta aaaaatacac ttcatctgcc
      361 tttccataaa atgggctata ggcctgtgtt tctttatata atggtttgga gtgataaata
      421 taatttgcct gtacacttat gccataatta ttcagtagtc tttcgttgtt ttttgaaatt
      481 aaaaattttg ttggtttata gccagtagtt ttttcccagt aagcactatt aatatcaatt
      541 ctataattgc tatcagttct attattggtt tgtgctatta attcagaact gttataaatg
      601 gttggcatta atgtaaaaaa acttccatca ctactagttt gtgctgctgc aaacccaaac
      661 tctttaccag gaatagttaa ataaattcct atgccctctg ctggttgtaa tgttctttta
      721 tctattactt tacctgttat aatatgattt tcatattctg gtaaatattt tggtgttgtt
      781 ggtgattgta aaatatttag ccaattaaat cttctccagc cttgtgttaa aattaaattg
      841 tctaatgcac tatctgtttc ttcattgata gtgttaaaat agtatgcagg ttcttctata
      901 tttcctttaa gttcagatgt aagccataag tattgctgaa tatctacatc attatttaat
      961 tgaaaagaat ctgttttaaa aaccgctaca gaaagattag tttctattgg ttgattagtg
     1021 ttggttaccc cattaaccat tatatttact ttttctctgt tgttaaatac agtattgtta
     1081 atgttcgttt ttaaagttgc tttttcggtt ggtcttttat acaataatct ttcagttact
     1141 ggcaagtcgt aatcattaaa aatagtaaga tggttaattc cctctttcaa taaattatta
     1201 tgtactgtaa aaactgcttg ttgattattt atactttgtg tttggttata ttgtatagtg
     1261 ttgttgttat gtataactaa tttaactgct ttattattca aataattact ggcaaataca
     1321 gtaatggttg tatgtgttga atttctggtt acttgcatta cataaccatc atttaaaatt
     1381 ttgggtaggt tataacttaa agtatcgttg gtgggggtaa atactaaagc cttataagta
     1441 ttagtattta ctggtgtaaa taaaaacgat ccaataccta aatggtttgc attaaactct
     1501 tgtatggttt cgttgttttg gtttactatt ttaccttttg tcattacgcc aaagccattg
     1561 ctgtttataa ctttaaatgc aattttactt tgtatgttat taaccaggtt tcccccttca
     1621 ggaaaaaatt gcacatcgta actaattgct ttttttgctg tatcaataga atttttttca
     1681 aaagcattaa ttacagatat ggttttttca aacataattt cttcgttata atttttcatc
     1741 caattggtat atgctctaaa aatataattg cctgttggta aggataatgg taaaacaaat
     1801 tgtccattac cagtgccatc tgttaaagca atttttgctt gaagtaaaga tttgttttca
     1861 gtatttaata actctacata ggcaatttta ctaatggtag taaatgtatt ttgagaagca
     1921 gaagtattgt atattttaaa ccaaactata tcacccgata aataaaaatt tctatcggta
     1981 tgtacaaata ttttttcttg aaaattattt aaaacgtact cattaaattt taatgaaaca
     2041 tcttttctat catcaacctg tgcaaatagt ttaaaactaa acgctaaaaa tagtaatacc
     2101 gaaaatgtaa gcttttgcat aattaacttt cttaaaatat aatttttctg ctttaatgtt
     2161 tttaccaaaa tgctggttta attgttgtgc cacctaaaac tctacaatct gtacattgtg
     2221 tagagcctgc taaataagct ccttgcatat ctggtgcaat gggtatatat cttttattgg
     2281 tataatacac tttcatgctg tctttattat ttgcaacaac agcaaattcg caagttgcag
     2341 gatatggttg cacaaaagtt ggaacattgg tatttaaaat ataaattctt ttttctgcag
     2401 ttgtggttgc actcacatat ccaattatag gttcattggg gtttgtaacg caatgcaaat
     2461 tgcctaaaag ttgtgttggt tgcacatcaa aaacagagcc tcctaattcg ctagattgtt
     2521 ttaattgttg ccaatactc
//
LOCUS       DAOQHP010000326         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_29619_length_2516_cov_3.661113, whole genome shotgun sequence.
ACCESSION   DAOQHP010000326 DAOQHP010000000
VERSION     DAOQHP010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29619_length_2516_cov_3.661113"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLY81_09265"
     CDS             complement(<1..1123)
                     /locus_tag="PLY81_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF30455.1"
                     /translation="MQSEKGIWKVIAASSVGTLIEWYDFYIFGSLAIIISEKFFPPEN
                     TTTAFLATLATFAAGFIVRPFGALFFGRLGDMIGRKYTFLLTLIIMGGSTFLIGCIPS
                     YQTFHFAPYVILFLRLLQGLALGGEYGGAATYVAEHAPEHKRGYYTSWIQTTATLGLL
                     VSLVVIITTQNSLSKADFNNWGWRLPFLLSIVLVLVSIIIRLKMKESPLFAKLKTEGK
                     TSINPIKESFGKRQNLKLVLLALFGATMGQGVIWYTGQFYTQTFLLKTCNLNAIQTNT
                     WLGVALILATPFFIFFGWLSDKIGRKVILLTGMLLAVFLYRPIFSELFSITNTKNKQE
                     VSTNIHISKTVVLNNSADSLITKTTTTNFTDNTVLVETEK"
     gene            complement(1165..1863)
                     /locus_tag="PLY81_09270"
     CDS             complement(1165..1863)
                     /locus_tag="PLY81_09270"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNF30456.1"
                     /translation="MQENLFFKLFKNKKLTATNYLFIAINLIPIWGVWFKNWDARIIF
                     IIFCLETVIIGLYNILKIFTASIFSNKNEIVNNSKVPKLAVGLFIIAFFIFHFGLFVF
                     VQMKIFLGVLSMNKMHIEMYDLFFNLRSTLPRYAQLLLLLFFISYGFGVIKEFVIPKQ
                     FKTVRLEKLMLEPYGRIITQQLVVIIGTFTLFINKDGKILILIFVLVKLYFEVFMDYK
                     VFLKDSYKNNTTSL"
     gene            1918..>2516
                     /locus_tag="PLY81_09275"
     CDS             1918..>2516
                     /locus_tag="PLY81_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNF30457.1"
                     /translation="MQKLTLAIHGGAGTILKNDLTPELERLYNDGLKQALQSGYAVLE
                     KNGTATDAVVAAVTSLENNILFNAGKGSVFTKNGEHEMDASIMEGKNLIAGAVAGVKN
                     IKNPIQLAQKIMLHSNHVFLSGQGAFEFAQQQQLAVENEDYFYSEFRYKQWQAVKETD
                     SYLLDHNSNETENLLKNKKFGTVGATACDAFGNLAAATST"
BASE COUNT          918 a          379 c          372 g          847 t
ORIGIN      
        1 atttttctgt ttcaactagt acagtgttat ctgtaaagtt tgtagttgtt gttttagtta
       61 ttaacgaatc ggcactatta tttaaaacta ctgttttgct tatatgaata tttgtggata
      121 cttcttgttt attttttgta ttagtgatag agaataattc agaaaatata ggcctataaa
      181 gaaatacagc caatagcata cctgttaaca aaattacttt tcttcctatt ttatcgctca
      241 gccagccaaa gaaaataaaa aatggagttg ctaatattaa tgcaactcca agccaagtat
      301 tggtttgaat agcatttaga ttacaagttt ttaataaaaa tgtttgagtg taaaattgcc
      361 ctgtgtacca aattacacct tgccccattg ttgcaccaaa taaagccaat aaaactaatt
      421 ttaaattttg tctttttcca aaactttctt taatgggatt aatgctcgtt ttgccttctg
      481 tttttagttt agcaaacaaa gggctttctt tcatttttaa ccttataata atggatacta
      541 acaccaacac aatacttaat aaaaaaggta gccgccaacc ccagttatta aaatctgctt
      601 tacttaagct gttttgagta gtaataataa ctactaaaga aaccaataag cctaatgttg
      661 cagtggtttg aatccagctt gtatagtagc ctcttttatg ttcaggtgca tgctctgcta
      721 cataggttgc agcaccacca tattcacctc ctaatgccaa gccctgtagt aatcttaaaa
      781 ataaaataac ataaggtgca aaatgaaaag tttgataaga tggaatacat ccaattaaaa
      841 atgtacttcc acccataata attaaggtga gtaaaaaagt atattttcta ccaatcatat
      901 cgcctagtct tccaaaaaat aaagctccaa atggcctcac aataaagcct gctgcaaagg
      961 ttgctaatgt tgctaaaaat gctgttgtag tgttttctgg aggaaaaaat ttttcagaaa
     1021 taataatagc taagcttcca aatatgtaga aatcgtacca ttctattaat gtaccaacgc
     1081 tagatgctgc aataactttc caaattcctt tttctgattg cataaatttt tattgttatt
     1141 taatctaatt aggttgtatg taatttaaag actggttgta ttattcttgt aagaatcttt
     1201 aaggaaaacc ttatagtcca taaagacttc aaaatacaat ttaactaaaa caaaaatgag
     1261 tattaatatt tttccatctt tatttataaa caaagtaaat gtaccaataa taactactaa
     1321 ttgttgcgta attattcttc catacggttc aagcattaac ttctctaatc taactgtttt
     1381 aaattgcttg ggtattacaa attcttttat tacaccaaac ccataactta taaaaaataa
     1441 taacaatagc agttgtgcat aacgaggtaa agtgcttctt aaattaaaaa acaaatcata
     1501 catttcaata tgcattttgt tcatacttaa aacacctaaa aatatcttca tttgtacaaa
     1561 aacaaataat ccaaaatgaa aaataaagaa cgctatgata aataagccaa ctgctagctt
     1621 tggcacttta ctattgttta caatttcatt tttattagaa aatatggagg cagtaaatat
     1681 ttttagaata ttgtaaaggc caattattac tgtttctaaa caaaaaatta taaaaattat
     1741 tctagcatcc caatttttaa accaaacgcc ccatataggt attaaattaa tggctataaa
     1801 caagtagttg gtagcggtta attttttatt tttaaaaagt ttaaagaata aattttcttg
     1861 catgaagtga actttttttc tttgatttgt gtttccaaat taatgaaaaa attatacatg
     1921 caaaaactta cactagcaat acacggtggt gcaggaacta ttttgaagaa cgacttaacc
     1981 cctgaacttg aaagattata taatgatggc ttaaaacaag cattgcagag cggttatgct
     2041 gtattagaaa aaaatggaac tgcaacagat gcagttgttg ctgctgtaac ttcattagaa
     2101 aataatattt tgtttaatgc aggtaaggga tcagtgttta caaaaaatgg cgaacatgaa
     2161 atggatgcct ctattatgga aggtaaaaat ttaatagctg gtgcagttgc aggtgtgaaa
     2221 aatattaaaa atccaattca gcttgcacaa aaaattatgc tgcatagcaa ccatgttttt
     2281 ttaagtgggc agggagcatt tgagtttgct caacaacaac aattagcagt agagaatgaa
     2341 gattattttt attctgaatt tagatataaa caatggcaag cagtaaagga aactgatagt
     2401 tatttgttag atcacaatag taatgaaact gaaaatttat taaaaaataa aaaatttgga
     2461 acagtagggg caactgcttg cgatgctttt ggcaaccttg ctgctgcaac aagtac
//
LOCUS       DAOQHP010000327         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_29715_length_2511_cov_4.256922, whole genome shotgun sequence.
ACCESSION   DAOQHP010000327 DAOQHP010000000
VERSION     DAOQHP010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29715_length_2511_cov_4.256922"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLY81_09280"
     CDS             complement(<1..591)
                     /locus_tag="PLY81_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNF30458.1"
                     /translation="MPDAWQAIVNSYKGAEAWKLYVVILLMFVNWGLEAKKWQMLVSG
                     IQNVSFFRAFRAIFTGQAIALNTFNGVGEYVGRVIYLNEGNRLRAIALSIVGSMSQII
                     ITLVMGLIGLLYVKFKILDETHHVQGLSKFWLDAMLISLGFWTLVFLVLYFQLSWLTK
                     LVEKIPFVAKYSFYIQKLEDFHYKELTKILLLSFFRY"
     gene            complement(782..2032)
                     /locus_tag="PLY81_09285"
     CDS             complement(782..2032)
                     /locus_tag="PLY81_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF30459.1"
                     /translation="MKNTDTVWFIAGFMLMLDLYAFQAIRVLTIGLHTKTRIVVQGSY
                     WFLSVAAILVLLSIPYLQTLQTSKFFRNYIFAIFVGLFLAKLLGSLFFVIDDIRRLFV
                     WVAGKFNTTKQIAENATNGPSISRSVFLSWLGLGAGATLFGTMLYGFSNRYNYQIKKV
                     SLSFKNLPNSFKGFKIVQISDIHSGSFQNKKAVEKGVEKILEQKPDMILFTGDLVNDS
                     YEEVIDYIDIFSRLKAPYGVYSILGNHDYGDYSTWDTLEAKIENIEKIKQAHSKMGWR
                     LLLNEHVAIEKNSESFALIGIENWGAKAYFPKYGKLQEAYNGTEKYAFKLLMSHDPSH
                     WDAEVRTKYTDIDLMLAGHTHGMQFGIENPYFKWSPVQYMYKQWAGLYEEGNQKLYVN
                     RGYGFIGYPGRVGILPEITVIELT"
     gene            2146..>2511
                     /locus_tag="PLY81_09290"
     CDS             2146..>2511
                     /locus_tag="PLY81_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF30460.1"
                     /translation="MQFTYYGHSCFSIIINGKHLLFDPFITYNELASHIDIESIKADY
                     IFISHGHADHIADCISIAKRTNALVICSWEIHEWLNKKEISNTHPMNTGGKKVFDFGT
                     VKCTVAQHSSGLPDGSYGGN"
BASE COUNT          870 a          439 c          343 g          859 t
ORIGIN      
        1 gtatctaaaa aaagaaagta gtaaaatttt tgttaattcc ttataatgaa aatcttctaa
       61 cttttgaata tagaaactat acttggctac aaatggaatt ttctctacta atttagtaag
      121 ccaggataat tgaaaatata aaactaaaaa aaccagtgtc caaaatccta atgatatcaa
      181 catggcatct aaccaaaact tacttaaacc ttgtacatga tgtgtttcat ctaatatttt
      241 aaacttaacg tatagtaaac caattaagcc catcactaaa gttatgatta tttgactcat
      301 acttcctaca atggataagg caatggctct taacctattt ccttcattta aataaattac
      361 tctgcctaca tattcgccta cgccattaaa agtatttaat gcaattgcct gccctgtaaa
      421 aatggctcta aaggctctaa agaaggaaac gttttgaata cccgaaacaa gcatttgcca
      481 tttctttgcc tctaaccccc agtttacaaa cattaataaa attactacat acaacttcca
      541 ggcttctgca cctttataag agtttacaat ggcttgccat gcatctggca agttgggttg
      601 ctcattaatt tgtttataaa tagaaaagct tagccataca aataaaattg ggccaataat
      661 atagttaaga aatatttttt tactcttctt cagtaattag tttttatatt attacaaatt
      721 acgaatatac tgttgagatt gtaaaataga attgctatat ttttttaata agagagagta
      781 attaggtgag ttctattaca gtaatttctg gtaaaattcc tactctacct gggtagccaa
      841 taaaaccata accacggttt acatataatt tttgatttcc ttcttcgtat aaaccagccc
      901 attgtttgta catatactgt acagggctcc atttaaaata aggattttct attccaaact
      961 gcattccatg cgtatgccct gcaagcatta aatcaatatc ggtatattta gttcttactt
     1021 cagcatccca atggctgggg tcgtggctca ttaataattt gaaggcatat ttttctgttc
     1081 cgttataagc ctcttgtaat tttccatatt tggggaaata agctttggca ccccagtttt
     1141 caataccaat taatgcaaaa gattcactat ttttttctat tgctacatgt tcgtttaaca
     1201 acaaacgcca acccattttt gaatgagctt gttttatttt ttcaatattt tctattttag
     1261 cttctaaagt atcccaggtt gaataatctc cataatcatg gttgcctaaa atactataaa
     1321 ctccataagg tgcttttagc ctactgaaaa tatctatata atcaatcact tcttcatagc
     1381 tgtcgtttac taaatctcct gtaaataaaa tcatatcggg cttttgctct aatatttttt
     1441 ccacgccttt ttctactgct tttttatttt gaaaactgcc actatgtata tcgctaattt
     1501 gaacaatttt aaatccttta aatgaattag gtaaattttt aaaagacaag gaaacttttt
     1561 ttatctgata attgtatcta ttactaaagc catataacat ggttccaaat aatgttgcac
     1621 ctgcacctaa gcctagccaa cttaaaaata cacttcttga aatggatgga ccattggttg
     1681 cattttcggc aatttgtttt gtggtattaa acttacctgc aacccaaaca aacaaccttc
     1741 ttatatcatc tatcacaaaa aataacgaac caagtaactt ggctaaaaac aagcctacaa
     1801 aaatggcgaa aatgtaattt ctgaaaaact tagatgtttg taaagtttgc aaatagggta
     1861 tagataataa aaccaaaata gcagccacac ttaaaaacca ataactacct tgtactacaa
     1921 ttcttgtttt agtatgtagg ccaatagtta aaactcttat agcctgaaag gcatacaaat
     1981 ctaacataag cataaatcct gcaataaacc aaactgtatc agtatttttc atgtatataa
     2041 acttatcaaa acattgcaaa gaaaaaaata ttcaaggttt aatttattta tacccgaaga
     2101 tttaacatta attgctgaaa tttggatgtc ttaaaaaata taactatgca atttacttat
     2161 tacggccatt cttgtttttc tataattata aatggcaaac accttttgtt cgaccctttt
     2221 attacctaca atgagttagc aagccatatt gatatagaaa gtataaaagc agactatata
     2281 tttattagtc atggccatgc agatcatatt gctgattgta ttagcattgc taaaagaacc
     2341 aatgccttgg ttatttgtag ttgggaaata catgaatggt tgaacaaaaa ggaaataagc
     2401 aatacgcacc ctatgaatac tggtggtaaa aaagtttttg attttggaac agtaaaatgc
     2461 actgttgcac aacatagcag tggattgcca gatggcagtt atggtggcaa c
//
LOCUS       DAOQHP010000328         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_29895_length_2502_cov_3.959134, whole genome shotgun sequence.
ACCESSION   DAOQHP010000328 DAOQHP010000000
VERSION     DAOQHP010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29895_length_2502_cov_3.959134"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLY81_09295"
     CDS             complement(<1..848)
                     /locus_tag="PLY81_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30461.1"
                     /translation="MKYWVTILLVLQGYIGNTQYNLVPNYSFEDSVVCPSDINGGPLP
                     APWYLPQNVPNKLCYFHSCSTSSNYSVPNNVGNTSFQYAKTGQGYAGVDVYNSLSFFD
                     GRYYLQTKLKSLLQIGGCYYAEFWVSLLNYSRIATNNIAMLVTDTAILVDASYDAQFG
                     IGLIPANPQIFNYGNPVITDTLNWVKISGVFTAQGGEEYLTIGNFKDDTNTDTLRVNS
                     GVNTYEKAAYYIDDIYLIPLDSMFLHADAGTDKTITQGDSVYIGSYINGINNITWYNS
                     TGSIIAT"
     gene            complement(850..1437)
                     /locus_tag="PLY81_09300"
     CDS             complement(850..1437)
                     /locus_tag="PLY81_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30462.1"
                     /translation="MKSLLILFISVFLWNNIVKAQPPCINGHINTDLSASLSAGLYYI
                     DTDVYVTSDITITDAELLVIGNVVFLSQNLYNAITNEHRIFTTSCEIRSTEFPDSFVT
                     NNYVAKPANKIIENNTLSVYPNPVNNNVTVKGNDIAYIEVYTIYGIKAYSSQHYNYKG
                     AAVINLPLKNLAQGMYILKVYDRQNKSLSRKLIKQ"
     gene            1614..>2502
                     /locus_tag="PLY81_09305"
     CDS             1614..>2502
                     /locus_tag="PLY81_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNF30463.1"
                     /translation="MPNHHPHHSLSDVHETVDTTQPRKGIKRFLAYVGPAYLVSVGYM
                     DPGNWATDLQGGAKFGYQLIWVLLMSNLMALLLQSLSARLGIVRRRDLAQANRETYPP
                     LINFCLYILAEIAIAACDLAEVLGMALGIYLLTGLPLIWGVSITVLDTFLLLWLQRFG
                     IRKLEAFIIALVIVIGLSFLIQIFLAKPNATELVKGFIPSSLNNEALYIAVGIIGATV
                     MPHNLYLHSALVQTRKIGSDTASIKKAIRFNIIDSTIALNAAFLVNAGILVLAASTFF
                     VTGHQNVTKIEDAHKLLSPL"
BASE COUNT          812 a          394 c          406 g          890 t
ORIGIN      
        1 gttgctatta tgctgcctgt gctgttgtac catgtaatat tattaatacc atttatatag
       61 ctgcctatat atacactatc cccctgtgtt atggttttat ctgtacctgc atctgcatgt
      121 aaaaacatac tgtctaaggg tattaaatat atatcatcta tgtaataggc tgctttttca
      181 taagtattta ctcctgaatt aactcttaga gtgtctgtat tagtatcatc tttaaaatta
      241 ccaatagtca aatattcctc cccaccctga gccgtaaata caccactaat ttttacccag
      301 ttcagggtat ctgtaattac aggattacca taattaaata tttgtggatt agcaggtatt
      361 aaaccaatac caaattgagc atcataactg gcatctacca atattgctgt atctgtaacg
      421 agcatagcta tattattggt agcaattctt gaataattta aaagagaaac ccagaactca
      481 gcataataac acccaccgat ctgtaataga cttttaagct ttgtttgaag ataatatcta
      541 ccatcaaaaa aggaaagact attatataca tccactcctg cataaccttg tcctgtttta
      601 gcatattgaa aactggtatt gccaacatta ttcggcacac tataatttga tgaagtggaa
      661 catgaatgaa aataacataa tttatttggt acattttgtg gtaagtacca aggggcgggc
      721 aatggtccac cattaatatc actaggacat acaacactat cctcaaagct gtaattaggt
      781 acaaggttat attgggtatt gccaatataa ccttgtaaaa ctaataatat cgttacccaa
      841 tatttcattt tattgcttta tcagcttacg acttaaactt ttgttttgcc tatcatatac
      901 ttttagtata tacattccct gtgccaaatt ttttaatggt aggttaataa ctgctgctcc
      961 tttataattg tagtgttgag agctatatgc ttttatacca taaatagtat atacttctat
     1021 ataagcaata tcatttcctt ttactgtaac attattattg acggggttgg ggtaaacact
     1081 tagggtatta ttttctataa tcttattggc aggtttagct acatagttat tagtaacaaa
     1141 gctatcagga aactcggtac ttctgatctc gcagcttgta gtaaatattc tatgctcatt
     1201 ggtaatggca ttgtacaggt tttggcttaa aaataccacg ttgccaatta ctaataattc
     1261 tgcatcggtt atagtaatat cgcttgtaac atacacatct gtatctatat aataaagacc
     1321 agcagaaaga gaagcactta aatctgtatt aatatgacca ttaatgcaag gcggttgtgc
     1381 cttaacaata ttattccata gaaaaacgct tataaaaaga atgagcagcg atttcataaa
     1441 cattattttt atgttagggt aaagttagtg ttttaatttt atgcactcat aataaaaatt
     1501 gcagcattgg agtggcttaa agtgtagtaa ttttattttt tttaactagt tgtatatagt
     1561 ataaaatagt ttaaactaat aattaaattt aaagttttat tttagttgta tatatgccca
     1621 accatcatcc tcatcattct ttaagcgatg tacatgaaac tgtagatact acacaaccca
     1681 gaaaaggtat taaacgtttt ttagcctatg taggcccagc gtatttggtt agcgtaggtt
     1741 atatggatcc aggaaattgg gcaacagatt tgcaaggagg agccaaattt ggataccaac
     1801 taatttgggt tttattaatg agcaatttaa tggcattatt acttcaaagt ttaagtgcta
     1861 ggttgggtat tgttagaaga agagatttgg cccaggctaa tagagaaact tatcctccac
     1921 taatcaattt ctgtttatac attttagccg aaattgctat tgctgcttgc gatttagccg
     1981 aagtactagg tatggcatta ggtatatatt tacttacagg cttgccttta atttgggggg
     2041 taagtattac tgtattagat acttttttac tactatggct acaacgtttt ggaataagaa
     2101 aattagaagc ttttattata gctttagtaa tagttattgg cttgtctttt ttaattcaaa
     2161 tatttttggc aaaaccaaat gcaacagagt tagtaaaagg ttttattcct tcatctctaa
     2221 ataacgaggc tttgtatatt gcagtaggta ttattggagc tacagtaatg ccacataatt
     2281 tatacttaca tagtgctttg gtacaaacaa gaaaaattgg tagcgatact gctagcatta
     2341 aaaaagcaat tcggtttaat attatagata gtacaattgc acttaatgca gcttttttag
     2401 ttaatgcagg tattttagtt ttagcagcat ctaccttttt tgtaacaggc catcaaaatg
     2461 taactaaaat tgaagatgca cataaattat tatctccatt at
//
LOCUS       DAOQHP010000329         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30165_length_2489_cov_3.113394, whole genome shotgun sequence.
ACCESSION   DAOQHP010000329 DAOQHP010000000
VERSION     DAOQHP010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30165_length_2489_cov_3.113394"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLY81_09310"
     CDS             <1..626
                     /locus_tag="PLY81_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF30464.1"
                     /translation="STSQTMIYPKAGIFTIQLQVKNPVGCTSDIFSKQVKVYVQPVID
                     AGPAIYVPTGTVVKMNPTVNDSSSSINFTWTPPGDFSNPTILRPLLTVNQNKIYTFSA
                     TGQGNCTATDTIMVVALKKIEVPNAFSPNGDGINDTWEIKNLKDYPIVTVEIFNRYGQ
                     KVYNSNRYSASWDGRVNGKPIPVGVYYYIIDFRGHYPTLTGSLTILR"
     gene            660..1667
                     /locus_tag="PLY81_09315"
     CDS             660..1667
                     /locus_tag="PLY81_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF30465.1"
                     /translation="MRVISKLFFLTVTVGLLSVSTLKAQVDPHFSQYYMYPSWLNPAL
                     TGAFDGDYRVTGIYRSQWGNITNPFATPGVSVDFNGNNSLNYGGSVMQQTAGNGGYGY
                     LTAYGNIAYTGVKFGATGTQRLVFGLQAGIIQRRFNPNKMQWGSQWDPSMGYNPNLPA
                     DILARTSATAFDAGAGVLYYDAQPGKKINVYGGFSAAHLTRADDKFSSNGTEKFPIRY
                     TVHGGVKIAINENFTLTPNFLYLRQRTSSQAMLGAYGQIKAPFETEFLFGLNYRFKDA
                     VSPYIGLYYKNMTLGLSYDINSSDLSKLQRGSNSFEISLSFIGRKRAKTPEVEFVCPR
                     L"
     gene            1710..>2489
                     /locus_tag="PLY81_09320"
     CDS             1710..>2489
                     /locus_tag="PLY81_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNF30466.1"
                     /translation="MKKLLLIFVSAFIALNSSAQIVYDYIKAADAYYEKADYYSAAEY
                     YEKYVNSANKKVKADAYNPYTVNALSKKQKVAVSNYQQAVYKLAECYRHLNYHVKAEP
                     LYAQASNFDKAVFPLAPYWHGKTLRALAKYSAADTVLTKFVANGSGGNEYMEDAGREL
                     KNLKFIQEQLVKPDLHLYTVKKSASGINTIGANYAPVKVNANTYLFTSTRNDSGAAKN
                     SAHYNRIYTASFTDGVLHEVKKADIPQSNNSHQGVVAITPNG"
BASE COUNT          865 a          380 c          434 g          810 t
ORIGIN      
        1 cttctacaag tcaaactatg atttatccga aagcaggtat ttttaccatt caattacaag
       61 taaaaaatcc tgttggttgt acttctgata ttttctcaaa acaagtaaaa gtatatgttc
      121 aaccagtaat tgatgcaggc cctgcaattt atgttcctac aggcacagtg gttaaaatga
      181 atccgactgt aaatgattct tcaagctcta ttaattttac atggacaccg ccaggcgatt
      241 tttcaaaccc aactatttta agacctttat taacagtaaa tcaaaataaa atttatactt
      301 tttctgcaac tggccaaggt aattgtactg ctacagatac aattatggtt gttgcattaa
      361 agaagataga agtacctaat gcattttcac caaatggaga tggcataaat gatacttggg
      421 aaattaaaaa tttaaaagat tatccaattg ttactgttga aatatttaat agatatggcc
      481 aaaaagtgta taatagtaat agatattctg ctagttggga tggaagagtg aatggaaaac
      541 ctattcctgt tggtgtgtac tattatatta ttgactttag aggacattat ccaacattaa
      601 ccggatcatt aacaatactt agataaaaca aattaaccta acaataaaag caaacgataa
      661 tgagagttat ttctaaatta ttttttctta ctgtaactgt gggcttgctt tcagtttcta
      721 cactaaaagc acaggtagat ccacatttct ctcaatatta tatgtaccca agttggctaa
      781 atccggcttt aacaggtgca tttgatggag attacagagt tactggtatt tatagaagcc
      841 aatggggtaa tattactaac ccttttgcta caccaggtgt ttctgttgat tttaatggca
      901 acaattcgct aaattatgga ggaagtgtaa tgcaacaaac tgctggaaat gggggctatg
      961 ggtatttaac agcttatggt aatattgctt atactggtgt taaatttggt gctacaggta
     1021 cacaaagatt ggtttttggg ttacaagcag gaattataca aagaagattt aatcctaata
     1081 aaatgcagtg gggtagccaa tgggatccta gtatgggcta taacccaaac ttacctgcag
     1141 atattttagc tagaacttcg gctactgctt ttgatgctgg tgctggtgtt ttatattacg
     1201 atgcacaacc aggtaaaaaa ataaatgttt atggtggatt ttctgctgca cacttaacca
     1261 gagcagatga caaatttagc tcaaatggta cagagaaatt ccctattcgt tataccgtac
     1321 atggaggtgt taaaatagct atcaacgaaa attttacatt aactcctaac ttcttatacc
     1381 tacgtcaaag aacttcttct caagctatgc ttggagctta tggtcaaata aaagcaccat
     1441 ttgaaacaga gtttttgttt ggattaaatt atagatttaa agatgccgtt tctccatata
     1501 ttggtttata ttataaaaac atgactttgg gtttaagtta cgacatcaat tcatctgatt
     1561 taagtaaact tcagagaggc tctaatagtt ttgaaatttc tttatctttt ataggaagaa
     1621 agagagcaaa aactccagaa gttgaatttg tttgtccaag attataattt tcgtttatac
     1681 tatattttta aacaggctaa tatcattaca tgaaaaagtt attgttaata tttgtttctg
     1741 cttttattgc tttaaacagt tctgcacaaa ttgtgtacga ttatattaaa gcagcagatg
     1801 cttattatga aaaagcagat tattattctg cagcagagta ttatgaaaaa tacgtaaata
     1861 gtgctaataa aaaagttaag gcagatgcct acaatcctta tacagtaaat gcactttcta
     1921 aaaaacaaaa agttgcagtt agtaattatc agcaagctgt ctataaactg gctgagtgtt
     1981 ataggcattt aaactatcat gtaaaggcag agcctttata tgcacaagca agtaattttg
     2041 ataaagcagt atttccttta gcaccatatt ggcatggtaa aacattaaga gctttagcaa
     2101 aatattctgc agcagatact gtactaacca aatttgttgc aaatggtagt ggaggtaacg
     2161 aatatatgga agatgcaggt agagaattga agaatttgaa attcattcaa gagcaacttg
     2221 taaaacctga cttgcattta tatactgtta aaaaatctgc atctggtatt aatactattg
     2281 gtgcaaatta tgcacctgta aaagtaaatg caaacactta tttatttacc tctacaagaa
     2341 acgatagtgg agctgccaaa aacagtgcac actataatag aatttacaca gcttctttta
     2401 cagatggtgt tttacacgaa gtgaaaaaag cagatattcc tcagtcaaat aattctcacc
     2461 aaggtgtagt agcaattaca ccaaatggt
//
LOCUS       DAOQHP010000330         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30210_length_2487_cov_4.713816, whole genome shotgun sequence.
ACCESSION   DAOQHP010000330 DAOQHP010000000
VERSION     DAOQHP010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30210_length_2487_cov_4.713816"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLY81_09325"
     CDS             complement(<1..270)
                     /locus_tag="PLY81_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNF30467.1"
                     /translation="MATVKKTKEKSIITEKSYQFLKEYINNASPVGFESSGQKIWLNY
                     IKPFVDTTFHDPYGTAVGVINPNDNFKVVIEAHADEISWYVNYITN"
     gene            complement(277..1191)
                     /locus_tag="PLY81_09330"
     CDS             complement(277..1191)
                     /locus_tag="PLY81_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30468.1"
                     /translation="MLKKLTDIFFYGNFFAGLCAVMLCIETNMQHGFALNGIHFYTII
                     FLGTAWYYTYTYVKNLPNELLDDRIIWYKKNTSSLKQTQNILLIFLIADIVFFVIKYE
                     ASFIKIASIQYGQIFLFPLVALTYTFNLLPFPEIKKLRRIGWLKPFIVGFVWSGFVSV
                     YPIIFYQIQTNIGNEIFAMPSGLLWLKNFMFISTLCVMFDLKDFEIDKKDGLKTFAVQ
                     FGVSNTIKYIIIPLVIICIAAFIIFVEQNHFPYQRILLNIIPYLFLILISFALLKKKR
                     KIIYYLFVIDGLMILKAICGILGMLIIK"
     gene            complement(1201..1683)
                     /locus_tag="PLY81_09335"
     CDS             complement(1201..1683)
                     /locus_tag="PLY81_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30469.1"
                     /translation="MKRLIFILLIFTTINSGCKKETVATKPSITIKKVGNDVVPINGS
                     MQIILSYTDKESDIDSIFIYRTRTNIKTVSGRLNDSTKLAVPSHENNRSGEIYLDFIT
                     DGVGRNDYQKYLISAIFPPVSGIPPKNESDSMLYKIIAVDKQKNKSDTAFYYPIVILR
                     "
     gene            1738..>2487
                     /locus_tag="PLY81_09340"
     CDS             1738..>2487
                     /locus_tag="PLY81_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNF30470.1"
                     /translation="MNKLCIDNILKINNEDSFKALALESFHFQFNQNLIYNKWCNLIG
                     VKPSNVSSIHQIPFLPISFFKTHSITTTNFEPTTIFESSKTTGNISSKHLIKDLAWYE
                     NSFNACFNQSYGNVEDYCILGLLPSYLERGNSSLVYMVEHFIKKSKHQLSGFYLHNYN
                     QLNIVLQQLEDEKIPTILIGVTFALLDFAEAFPMPLNHTTILETGGMKGRKKEILRTE
                     VHSILKNAFQTKNIHSEYGMTELLSQAYSTQN"
BASE COUNT          904 a          347 c          319 g          917 t
ORIGIN      
        1 gttggtgata taatttacat accaacttat ttcgtcagca tgggcttcaa ttactacttt
       61 aaaattatca tttgggttta ttacacctac agcagtgcca taagggtcat gaaaagtggt
      121 atccacaaat ggtttaatat aatttaacca aattttttga ccagaacttt caaatccaac
      181 aggtgatgcg ttattaatat actcttttag aaattgatac gatttttcgg tgatgattga
      241 tttttctttt gtttttttta cagttgccat gttttattat ttaatgataa gcatacctaa
      301 aatgccacaa attgctttta aaatcattaa gccatctatc acaaaaaggt aataaattat
      361 ttttcttttc ttttttaata atgcaaatga aattaatatt agaaataagt aaggaataat
      421 gtttaataaa attctttggt aaggaaaatg attttgctct acaaaaatta taaatgcagc
      481 aatacaaata ataaccagag gtataataat atatttaata gtattactaa cgccaaattg
      541 tactgcaaat gttttaagcc catctttttt gtctatttca aaatctttca aatcaaacat
      601 tacacaaagt gtagaaataa acatgaaatt ttttaaccat aacaaaccag aaggcattgc
      661 aaaaatttca ttaccaatat ttgtttgaat ttggtaaaaa ataatagggt aaacagatac
      721 aaacccactc catacaaagc caactataaa tggctttaac caaccaattc ttcttaattt
      781 tttaatctct ggaaaaggta gtaagttaaa agtataagta agtgcaacca atgggaaaag
      841 aaaaatttgc ccatattgta tgcttgctat tttaataaat gaagcctcat actttatcac
      901 aaaaaaaaca atatctgcaa ttaaaaatat taaaagtata ttttgtgttt gttttaagga
      961 ggatgtattt tttttatacc aaatgattct atcgtctaaa agttcgttag gtaaattttt
     1021 tacataagtg tatgtataat accatgcagt gcctaaaaaa attattgtat aaaaatgaat
     1081 gccatttagt gcaaacccat gctgcatatt ggtttcaatg caaagcatta cagcacaaag
     1141 ccctgcaaag aaatttccat aaaaaaaaat atctgttaat tttttcagca tgttttcaaa
     1201 ttatcttaaa attacaatcg gataataaaa tgctgtatcg cttttatttt tttgtttatc
     1261 aactgcaata attttatata acatactatc tgactcattt tttggtggta tgccagaaac
     1321 aggtggaaaa atggctgata tcaggtattt ttgataatca tttcttccta caccatctgt
     1381 aataaaatct aaataaattt cgccacttct attattttca tgtgaaggaa cggctaattt
     1441 agtactatca tttaaccttc cagaaacagt tttaatattt gttcttgttc tataaataaa
     1501 aatggaatct atatcagatt ctttatcggt ataacttaaa ataatttgca ttgaaccatt
     1561 tattggtact acatcatttc ctactttttt aattgttata cttggctttg ttgctacagt
     1621 ttctttttta cagccactat ttattgtagt aaaaataagc aatatgaaaa ttaatcgttt
     1681 catttgtatt ttgtttctat tccttacaaa actaatgttt aattctttaa atatatcatg
     1741 aataaacttt gtattgataa tattttaaaa attaataatg aagacagttt taaagccctt
     1801 gctttagaaa gttttcattt tcaattcaat caaaatttaa tatataataa gtggtgcaat
     1861 ttaattgggg ttaagccaag caatgtatct tctatccatc aaattccatt tttgccaatt
     1921 tcatttttta aaactcattc cattactact accaattttg aacctactac aatttttgaa
     1981 agtagtaaaa caactggaaa tattagtagc aaacatttaa ttaaagattt agcctggtac
     2041 gagaatagtt ttaatgcttg ttttaatcaa tcttatggaa atgttgaaga ctattgcatt
     2101 ttaggcttat taccatctta tcttgaaaga ggtaattctt ctttggttta tatggtggaa
     2161 cattttatta aaaaaagtaa acaccaattg agtggttttt atttgcataa ctacaaccag
     2221 ttaaatattg ttttacagca attagaagat gaaaaaattc ccactatttt aattggtgta
     2281 acttttgcgt tattagattt tgccgaagca tttccgatgc cattaaacca tactacaatt
     2341 ttagaaacag gtggtatgaa aggtagaaaa aaagaaatat tgagaactga agtgcatagt
     2401 attttaaaaa atgcttttca aacaaaaaat atacatagtg aatacggaat gacagaactt
     2461 ttatctcaag cctattctac acaaaat
//
LOCUS       DAOQHP010000331         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30527_length_2473_cov_3.558726, whole genome shotgun sequence.
ACCESSION   DAOQHP010000331 DAOQHP010000000
VERSION     DAOQHP010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30527_length_2473_cov_3.558726"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLY81_09345"
     CDS             <1..642
                     /locus_tag="PLY81_09345"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNF30471.1"
                     /translation="KTWTSLLKENGIKVVHVVSSSKFAQKAEAAGCDAVVAEGFEAGG
                     HNGREETTTMVLIPAVKKAISIPLIAAGGIATGKQMLAAMILGADAVQIGSRFVCTPE
                     ASSHQNFKESILNANEGDTMLHLKKTVPVRLLKNKFSETIKEAENRGASAEELNEILG
                     RARAKKGMFEGDLQEGELEIGQVSSIIHEIKPAAEIVQEIWNEFETTLKNPFK"
     gene            791..1180
                     /locus_tag="PLY81_09350"
     CDS             791..1180
                     /locus_tag="PLY81_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF30472.1"
                     /translation="MKNILLTITSLFLVFMVSAKTIKSKGEPVVVGNVTDAVSKKPVA
                     EVTVTAIHLTTKSEHQISTDINGNFKINQLPTGNYKIKFEKNNYKPVEKNNITIKQDS
                     TSKINIEITEKEETTYESIIWDLRFGI"
     gene            complement(1187..1531)
                     /locus_tag="PLY81_09355"
     CDS             complement(1187..1531)
                     /locus_tag="PLY81_09355"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30473.1"
                     /translation="MKKIYILFLGIFLIVGTSFTVPFGSGNDVEIKTKIVNFYPNPAT
                     SVIYFEYTKALDKGCTIQLYNFIGRKVHEVVVTGSKTTVNLDGYYRGIYVFQLRDKSG
                     KIIESGKFQVVK"
     gene            1727..>2473
                     /locus_tag="PLY81_09360"
     CDS             1727..>2473
                     /locus_tag="PLY81_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNF30474.1"
                     /translation="MISRRNIRVKVMQLLYVLESSGDKNFFKDPITQLQKNINKTNEL
                     LVYLIYNLTEVIRYVEIDAKNRASKHLPSYQDLNVNTKIAGNTILWGLLENNYIKDAF
                     IQYKCSSMVNTELIRKLYLQLIETEEYKTYIANQQRDKKEDKVIVDFVFNSLMLPNEL
                     FDEHIDELFSNWDDDAELMQQLVNAFIQKPGNDLASLLPEETLSFGKLLLKTVIEKNS
                     HTLETIKPKLKNWDSDRIAVLDMILMQMGIC"
BASE COUNT         1014 a          333 c          374 g          752 t
ORIGIN      
        1 aaaacatgga ctagcttatt aaaagaaaat ggaattaaag tagtgcatgt agtaagcagt
       61 agcaagtttg cacaaaaagc agaagcagca ggctgtgatg ctgttgtagc agaaggtttt
      121 gaagcaggtg gacataatgg aagagaagaa acaaccacaa tggttttaat tcctgcagta
      181 aaaaaagcaa ttagcattcc tttaatagca gcaggtggta ttgcaacagg taaacaaatg
      241 ttggctgcaa tgattttagg tgcagatgct gtacaaatag gcagtaggtt tgtttgtact
      301 cctgaagcat ccagtcatca aaattttaaa gagtcaattc ttaatgccaa cgaaggcgat
      361 actatgttgc atttaaaaaa aacagttcct gtacgtttat taaaaaataa attttctgaa
      421 accattaaag aagcagaaaa tagaggagct tcagccgaag aattgaatga aattttaggc
      481 agagcaagag ccaaaaaagg aatgtttgaa ggtgacttac aagagggtga attagaaata
      541 ggacaagtaa gcagcattat acatgaaata aaacctgctg cagaaattgt acaagaaatt
      601 tggaatgaat ttgaaactac tttaaagaat ccttttaaat aataaccatc taaatttaag
      661 ttctatatct acaatacttt tttcatttgc aaaatgttaa aatttgcaaa acatttaacc
      721 atttacacaa aaggtagtaa tagataaaga aaaaaaatta actttcaatc ttaatttcaa
      781 aaattaattt atgaaaaata tattacttac tattaccagc ttatttttag tatttatggt
      841 tagtgctaaa actataaaaa gtaaaggaga acctgttgta gtaggtaatg taacagatgc
      901 agtttccaaa aagccagtag cagaagttac tgtaacagca attcatttaa ctactaaatc
      961 agagcatcaa attagcaccg atataaatgg aaattttaaa ataaatcaac ttcctacagg
     1021 caactacaaa ataaaattcg aaaaaaacaa ttataagcct gtagagaaaa ataatataac
     1081 gattaagcaa gatagtactt caaaaataaa tattgaaatt accgaaaaag aagaaacaac
     1141 ctacgaaagt attatatggg atttgaggtt tggtatataa cacaaactat ttaacaacct
     1201 gaaattttcc actttcaatt attttaccac tcttatctct taattgaaaa acataaattc
     1261 ctctgtaata gccatctaaa ttaacggttg ttttactgcc agttaccacc acttcatgta
     1321 ctttacgacc aataaaatta tacaattgta tagtacaacc tttatctaaa gctttagtgt
     1381 attcaaaata aataacagat gttgccggat tgggataaaa atttacaatt ttagttttaa
     1441 tttctacatc attaccacta ccaaatggca cagtaaaaga tgtgccaaca attaaaaaaa
     1501 tgcctaaaaa tagaatgtag atttttttca tattaaatat acatttatga aggtaaataa
     1561 acattttaaa tttcccaaaa cttatttgtt taacagctaa cgaaaattca aaaaaaatat
     1621 tatgtaacat taaataataa caatattttt tttaatcaaa actttaacta ttaagccaat
     1681 aaaccccctt atcttcgcag ccctaaaata atgctcttta tattaaatga ttagtagaag
     1741 aaatatacgg gttaaagtaa tgcagctttt atatgtttta gaaagcagtg gcgataaaaa
     1801 cttttttaaa gaccctataa cacaattaca aaaaaatatt aataaaacaa acgaactatt
     1861 ggtgtactta atttataacc taactgaggt tataagatat gtagaaattg atgcaaaaaa
     1921 cagagctagt aaacaccttc catcttatca agatttgaat gtaaatacta aaattgctgg
     1981 caatactata ttgtggggtt tacttgaaaa caattatatt aaagatgcat ttatacagta
     2041 taaatgttct agtatggtta atacagagct tattagaaaa ttgtatttac agctaataga
     2101 aacagaagag tataaaacct atatagctaa tcaacaaaga gataaaaaag aagacaaagt
     2161 aattgtggac tttgtattta atagtttaat gttacctaat gaattgtttg atgaacatat
     2221 agatgaactt ttttctaact gggatgacga tgcagaattg atgcaacaac ttgtaaatgc
     2281 ctttatacaa aagcctggca atgatttagc aagcttgtta cctgaagaaa ctttaagttt
     2341 tggcaaattg cttttaaaaa ctgttattga aaaaaacagc catactttag agacaataaa
     2401 accaaaatta aaaaattggg atagcgatag aattgcagtt ttagatatga ttttaatgca
     2461 aatgggtata tgt
//
LOCUS       DAOQHP010000332         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30651_length_2466_cov_4.714226, whole genome shotgun sequence.
ACCESSION   DAOQHP010000332 DAOQHP010000000
VERSION     DAOQHP010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30651_length_2466_cov_4.714226"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLY81_09365"
     CDS             complement(<1..546)
                     /locus_tag="PLY81_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNF30475.1"
                     /translation="MVIFGIDNLLRSNCSWKKDRIALVTNHAATTNQLQPSRKLLLQN
                     GFNLVKLFSPEHGLNLMGEDGVKINNSTDELTKLPIISLYNQQLKPLEKDIDDIDIIL
                     FDIPDIGCRFYTYLWTMTYILEAAAQFNKPIITLDRPNPISGNFELAEGCLLDEKNCS
                     SFIGRFSIPIRHCCTLAELANY"
     gene            complement(559..1083)
                     /locus_tag="PLY81_09370"
     CDS             complement(559..1083)
                     /locus_tag="PLY81_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNF30476.1"
                     /translation="MAKNYIIIAILMIHKKYNMPSSTPIKRNEILQLISREHHYGLLL
                     SWKIREGMKKNVEINRITDYVNWFWESHLMHHFLIEEKFVFSILPTENNLVIQAINEH
                     ENLRKLFNEPEKNVETLKLIEKNLDQHIRFEERVLFNEVQLVATNEQLELIALNHTHT
                     FSDNWKDEFWATAK"
     gene            1381..>2466
                     /locus_tag="PLY81_09375"
     CDS             1381..>2466
                     /locus_tag="PLY81_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF30477.1"
                     /translation="MKLKSLEIKGFKSFADKTVVSFDEGITGIIGPNGCGKSNIIDSI
                     RWVIGEQKISALRSENLEALVFNGSKTRSASGLAEVSLTFENTKNLLPTEFSTVTVTR
                     RFYKNGDSEYRLNDVQCRLKDIHNLFLDTGVSNDSYAIIELGMVDDIIKDKDNSRRKM
                     LEQAAGITIYKTRKKEAKNKLDATEQDLSRIEDILFEINNQLKSLENQAKKAEKFYEI
                     KNEYKEIAIELAKASLEGFNLTYKELNEQQESETNRKLQLDAQIATQDAALEQEKVGM
                     IEKERVLQSMQYEFNELLQKLRTKENDKNLASQKLHYLKEKESSLIEFLNKAEGQLKN
                     TKDSIEFTQLQLKEESDKLNDLTEKVTS"
BASE COUNT          935 a          319 c          377 g          835 t
ORIGIN      
        1 ataatttgca agttctgcca aggtacagca atgacgaatt ggaatactaa atctaccaat
       61 aaaggaagaa cagtttttct catctaaaag gcaaccctct gctaattcaa aattgccaga
      121 aattgggtta ggcctgtcta aagttattat tggtttatta aattgtgctg cagcttctaa
      181 aatataagtc atcgtccaca aataggtata aaatctacag cctatatcag gtatatcaaa
      241 caaaataata tcaatatcat caatatcttt ttctaatggt tttagttgtt ggttatacaa
      301 actaataatg ggcaatttag taagttcatc ggtgctatta tttattttta ccccatcttc
      361 tcccattaag tttaagccat gttctggcga aaataatttt accaaattaa aaccattttg
      421 taataacaat ttcctacttg gttgcagttg gtttgttgta gcagcatggt tagtaaccaa
      481 agcaattcta tcttttttcc agctacagtt tgaacgcaat aaattatcta ttccaaatat
      541 aaccatagag atgttgtgtt attttgctgt tgcccaaaat tcatctttcc agttatcaga
      601 aaaggtatgt gtatggttta atgcaattaa ttctaattgt tcgttggtgg caactaattg
      661 cacttcgtta aacaaaactc tttcttcaaa ccttatatgt tggtctaaat ttttttcaat
      721 taattttagt gtttctacat ttttttcagg ttcgttaaat aactttctta aattttcgtg
      781 ttcattaatt gcctgaatta ctaaattgtt ttcagttggt aaaattgaaa aaacaaattt
      841 ttcttctatc aaaaaatgat gcattaaatg gctctcccaa aaccagttta cataatccgt
      901 tattctgttt atttcaacat tttttttcat tccttctcta attttccagc taagtaataa
      961 accataatga tgttctctac ttattagttg cagtatttcg tttcgtttaa ttggtgtgga
     1021 ggatggcata ttatattttt tatgaatcat taatatagca attataatgt aattttttgc
     1081 catttacaag ccaacttttt ataattgtta tttggttagt atcaaattga ttattaatac
     1141 attttgagat ttattttatt ttatattctg aaattataat aaatagtact agagttaata
     1201 tagaaaattt aaatgaatag taacgtagta tttaattcat aatcaaatac ttattgttta
     1261 ttgaataatt attaattaca aatacttttt ctcacctttt atacacactg cataattggt
     1321 gttttaataa gtagcttaaa acaattatgt tcgcaggtac ttaaaaaatg aagaagtagc
     1381 atgaaactaa aatcgttaga aataaaaggg tttaaaagtt ttgcagataa aactgtggta
     1441 agttttgatg aaggtattac aggaattatt ggacctaatg gctgcggaaa aagcaatata
     1501 attgatagca ttagatgggt aattggcgaa caaaaaatat cggcattaag aagtgagaat
     1561 ttagaagctt tggtttttaa tggctctaaa acaagaagtg caagtggttt agcagaagta
     1621 agcttgactt ttgaaaatac aaaaaacctt ttaccaaccg agttttctac agttactgtt
     1681 actagaagat tttataaaaa tggtgatagt gaatatagat taaatgatgt gcaatgccgt
     1741 ttaaaagata tacataattt atttttagat acaggtgtta gcaacgatag ttatgcaatt
     1801 attgaattag gaatggtaga tgatattatt aaagataaag ataatagccg tagaaaaatg
     1861 ttagaacaag cagccggcat tactatctac aaaacacgaa aaaaagaagc aaagaataaa
     1921 ttagatgcta cagaacaaga tttgtctaga attgaggata ttttatttga aattaacaat
     1981 caattaaaat ctttagaaaa ccaagcaaaa aaagcagaga aattttatga aataaaaaac
     2041 gagtataaag aaatagcaat tgaattagcc aaagcatctt tagaaggatt taatttaacc
     2101 tataaagagc taaatgaaca acaagaatca gagacaaaca gaaaactaca attagatgct
     2161 caaattgcaa cacaagatgc agctttagaa caagaaaaag ttggaatgat tgaaaaagag
     2221 agagtgttac aaagcatgca atatgagttt aacgagcttt tacaaaaatt aagaaccaaa
     2281 gagaacgaca aaaatttagc ttctcaaaaa ttacactatc taaaagaaaa agaaagcagt
     2341 ttaattgaat ttttaaataa agccgaaggc caattaaaaa atacaaagga tagtattgag
     2401 tttacacagc tacaattgaa agaagagagt gataaactaa atgatttaac agaaaaagtt
     2461 actagc
//
LOCUS       DAOQHP010000333         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30771_length_2461_cov_3.477556, whole genome shotgun sequence.
ACCESSION   DAOQHP010000333 DAOQHP010000000
VERSION     DAOQHP010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30771_length_2461_cov_3.477556"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLY81_09380"
     CDS             complement(<1..651)
                     /locus_tag="PLY81_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF30478.1"
                     /translation="MAIKSIIIDDERLARTELKKLLQEHKDIEIIDEAANVDDGIMKI
                     ETLSPDLIFLDIQMPGKSGFDLLAEVEKAPRVIFTTAFDEYAIKAFEVNALDYLLKPI
                     EPKRLSDAIQKLQYEIQKEKSGLLNGRGPLTEEDQVFVKDGERCWFVKLHEIRLFESV
                     GNYAKVFFSTNKPLILKSLNALEERLDERTFFRANRKHIINLRWIEKIEPYFNGGLL"
     gene            complement(689..1732)
                     /locus_tag="PLY81_09385"
     CDS             complement(689..1732)
                     /locus_tag="PLY81_09385"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF30479.1"
                     /translation="MKKKLVNYWLFQLGGWLLYALSMVFFSYLFRNNMDGIFWKRILL
                     FVFMGVAFTHVLRFCIHQFKLMPPIHGSKWINFFILVIFICIANSFVSSALIQYLGWY
                     RKEFNGTIGKRFIFTLFDSSPILLVWTSIYYVWHYVEDARRTEIDKIKLESVVKELEL
                     KTIKSHINPHFIFNALNSIRALVDENPIRAREAVTQLSNLLRSSMQTDKAETTSLEKE
                     LDIVKDYLALEHIRFEDRLQVEYEIDEDTLDQQIPPMMLQTLIENAIKHGIGKSKEGG
                     LIKIISRFIENHHELIVHNTGLIGNDYQQDGFGISSTKNRLALLFGKNASFNLTDDKI
                     TNKVIATIQLPIS"
     gene            1968..2285
                     /locus_tag="PLY81_09390"
     CDS             1968..2285
                     /locus_tag="PLY81_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNF30480.1"
                     /translation="MVVKIKEKSLWAKMAAYNMKASQMAIVFGNTIHLHNVTREEFLS
                     NRRWLRHEVAHVRQYQQKGFIRFILSYLFETFNMSYEFNKYEVEARKKETDLKVLEGV
                     EFI"
     gene            complement(2278..>2461)
                     /locus_tag="PLY81_09395"
     CDS             complement(2278..>2461)
                     /locus_tag="PLY81_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30481.1"
                     /translation="VYTLATALSLMMFYVLAMQCMSTLAIVKRETRTWKWPIFQFVYM
                     TLLAYSMSWVVYEIFK"
BASE COUNT          839 a          369 c          331 g          922 t
ORIGIN      
        1 caacaagcca ccattaaaat aaggttcaat tttttctatc caacgtaagt taataatatg
       61 ttttctgttt gctctaaaaa aagttctttc atcaagtctt tcttctaaag cgtttaaaga
      121 ttttaatatt aatggcttgt tagtactaaa aaatactttt gcataattgc caacactctc
      181 aaataatctt atttcatgca attttacaaa ccaacacctt tcaccatctt ttacaaaaac
      241 ctggtcttct tctgttaaag gtccacggcc atttaataaa ccagattttt ctttttgaat
      301 ttcgtattgt aatttttgaa tggcatcgct taatcttttt ggctcaatag gctttaataa
      361 atagtctaaa gcatttacct caaaagcttt aattgcatat tcatcaaagg cagtagtaaa
      421 aattactctg ggggcttttt caacttctgc taataaatca aatccagatt tgcctggcat
      481 ttgaatatct aaaaaaatta aatcggggct aagagtttca attttcatta ttccatcatc
      541 tacatttgct gcttcgtcta taatttcaat atctttatgt tcttgtaata atttttttaa
      601 ctctgttctt gcaagtcttt catcatctat aattattgat tttattgcca tacttttatt
      661 ttaataaatt aaataatttg atattgtttt atgaaattgg tagttggatt gtagctatta
      721 ctttattggt tattttatca tctgttaaat taaaggatgc atttttgcca aaaagcaatg
      781 ccagcctgtt tttagtacta gaaataccaa atccatcctg ttggtaatca tttccaatta
      841 agcctgtatt atgcactata agctcatgat ggttttcaat aaatcgagaa ataattttaa
      901 ttaacccacc ttcttttgat ttacctattc catgtttaat tgcattttca attaaggttt
      961 gaagcatcat tggtggaatt tgttgatcta aagtatcttc atctatttca tattcaactt
     1021 gtagtctgtc ttcaaatctt atatgttcta atgctaaata atcttttaca atatccagtt
     1081 ctttttctaa agatgtagtt tctgctttat ctgtttgcat actacttcta agcaaattgc
     1141 ttaattgggt aactgcttct cttgcacgta taggattttc atcaaccaat gccctaatgc
     1201 tatttaaagc attaaaaata aaatgcggat taatatggct tttaatagtt tttaactcta
     1261 gttcttttac tacactttct aattttattt tatctatttc tgttcttctt gcatcttcca
     1321 catagtgcca tacataataa atacttgtcc atactaataa aataggagac gagtcgaaca
     1381 aagtaaaaat aaatcgttta ccaatggttc cattaaactc ttttctgtac caaccaaggt
     1441 attgaattaa ggcactgcta acaaaactat tggctataca aataaatatt actagtataa
     1501 aaaagtttat ccatttactg ccatgtatgg gtggcattaa tttaaattga tgaatacaaa
     1561 agcgtaatac atgtgtaaat gcaacaccca taaaaacaaa taataatatt cttttccaaa
     1621 aaataccatc catattgttt ctaaataggt aagagaagaa taccattgaa agggcataaa
     1681 gtagccatcc tcctaattga aacagccaat aatttacaag cttctttttc atgacctaca
     1741 aatgtaaaaa aatgtaacta ctaaaaataa tgtgttaatg ataagtggtt ttgatagttt
     1801 gttgatggta gaaatatggt ttgattggat ttattctttc agtttaaatc aatttttttt
     1861 tattaataga ttaatttaaa attaaattca taattttttt aaagtatttt tattataatt
     1921 gtaaaagtat ttctatacta agttttttat aaggtaaatt gttaaccatg gttgtgaaga
     1981 ttaaagaaaa atcattgtgg gctaaaatgg ctgcatataa catgaaagct tctcaaatgg
     2041 ctatagtttt tggtaatact attcatttac ataatgtaac cagagaagag tttttaagta
     2101 acagaagatg gttaaggcac gaagtggctc atgtaaggca atatcagcaa aaaggattta
     2161 taaggtttat tttatcctat ctttttgaaa cttttaatat gagttacgag tttaataaat
     2221 atgaagtaga agccaggaaa aaagaaactg atttaaaagt gttagaaggg gtggagttta
     2281 tttaaaaatt tcgtacacaa cccaactcat agagtaagct aaaagcgtca tatatacaaa
     2341 ctgaaaaatt ggccacttcc aggttctagt ttctctttta acaatagcta aagtactcat
     2401 acattgcata gcaagcacat aaaacatcat taaagataat gctgttgcaa gggtataaac
     2461 t
//
LOCUS       DAOQHP010000334         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30803_length_2459_cov_4.313644, whole genome shotgun sequence.
ACCESSION   DAOQHP010000334 DAOQHP010000000
VERSION     DAOQHP010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30803_length_2459_cov_4.313644"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLY81_09400"
     CDS             complement(<1..788)
                     /locus_tag="PLY81_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF30482.1"
                     /translation="MKAALIKLHIAVFLWGFTGVLGKAIHLNEGLLVWYRLIITIFTL
                     AVFFIFSNKNIKVPKQSIVHYAAIGVIIALHWVCFYGSIKYANVSIALVCLSSAGVMT
                     AFLEPIMTGKKIVINEVVLGLVAVAGIYLIFHFDVQYRTGIMLGVLATLFSVIFSILN
                     KKAILKVEPKTLMFYQLIGGFVVLSLLLPAYLNYFPVKTILPSLSDLVWLIVLSLVCT
                     ILAMDLSLQALKKISAFTQNLTLNLEPVYGIILAFVVYQENKELG"
     gene            complement(847..1590)
                     /locus_tag="PLY81_09405"
     CDS             complement(847..1590)
                     /locus_tag="PLY81_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNF30483.1"
                     /translation="MEKIVIIPTYNEKENIANILQAIFSLNQQFHVLVIDDGSPDGTA
                     QIVKDLFAKYPNQLFIEERTGKLGLGTAYIHGFKWCLKNGYEFIFEMDADFSHNPKDL
                     INLYNACKEGNADVAIGSRYVPGGKIENWPLDRHIYSRGGAIYTKLITWMPVNDPTAG
                     FVCYKRKVLETINLNTINFVGYAFQIEMKFAAWKLGFKIKEVPIVFIDRKEGVSKMSK
                     GIIKEGVFGVLSMQWQSILGSYNKLKRRK"
     gene            complement(1684..2454)
                     /locus_tag="PLY81_09410"
     CDS             complement(1684..2454)
                     /locus_tag="PLY81_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF30484.1"
                     /translation="MKLQLINPIAFIDLETTGINISSDRIIEIAVVKILPDGTKQVKR
                     KLINPEMPISAGATQVHGITDEMVKDAPTFKQVANEVKQFMEGCDIGGYNSNRFDMPL
                     LIEEFLRVGINFVIQGRKFVDVQKVFHLMEQRTLTAAYKFYCDKNLEDAHSAEADATA
                     TWEILLAQVERYEQLGTTIESIVKFTGEDDIIDFARRFIRVKGVEVFNFGKHKGKPVE
                     QVIKEEPQYYDWMMKGDFAMDTKQKFTEILNRTLLKKG"
BASE COUNT          846 a          453 c          288 g          872 t
ORIGIN      
        1 cctaattctt tattttcctg atatacaaca aaggctaaaa taataccata tactggttct
       61 aaatttagcg taaggttttg tgtaaatgcc gatatttttt tgagtgcttg caatgaaaga
      121 tccattgcaa gtatggtaca aactaaactt aaaactataa gccaaactaa atctgataaa
      181 gaaggcaaaa tagttttaac aggaaaataa tttaaataag ccggaagaag caaagacaga
      241 actacaaacc ctcctataag ctgataaaac attagcgttt taggttcaac tttaaggata
      301 gcttttttat ttaaaataga aaaaattaca ctaaataaag ttgctaaaac acccaacata
      361 attcctgttc tgtactgtac atcaaaatga aaaattaaat aaatacctgc aactgcaacc
      421 aagcctaaca ctacttcatt tataacaatt tttttacctg tcataatagg ctctaaaaaa
      481 gcagtcatta cacctgcaga ggataaacaa actaaagcaa tagaaacatt ggcatatttt
      541 atactaccat aaaaacaaac ccagtgcaat gcaataatta cacctatggc agcatagtgt
      601 acaatagatt gtttgggtac ttttatattt ttattagaga agataaaaaa aacagcaagt
      661 gtgaaaatgg taataattaa tctataccaa acaagcaagc cttcatttaa atgaattgct
      721 ttgcctaaca caccggtaaa cccccaaaga aaaacagcta tatgtagttt tattaatgct
      781 gctttcattt aaaaaataag ataattgtaa ttgttagttg aaaatacaac aattagtagt
      841 aaaaaattat tttcttcttt taagtttatt ataactacct aaaatgcttt gccattgcat
      901 acttaataca ccaaaaacac cttctttaat aatgccttta ctcattttac ttacaccttc
      961 ttttctatca ataaaaacaa ttggtacttc tttaatttta aaacctaatt tccatgcagc
     1021 aaacttcatc tctatctgaa aagcataccc cacaaaattt attgtattta agttaattgt
     1081 ttctaatact tttcttttat aacatacaaa accagcagtt gggtcgttta caggcatcca
     1141 tgtaattagt tttgtataga ttgccccacc tcttgagtaa atatgcctat ctaaaggcca
     1201 attttcaatt ttacctccag gtacatacct gctaccaatg gctacatctg catttccttc
     1261 tttacaggca ttatataagt ttattaaatc ttttgggtta tgagaaaaat cggcatccat
     1321 ttcaaaaata aattcgtacc cattttttaa gcaccactta aacccatgaa tataagctgt
     1381 acctaaacca agttttcctg ttctttcttc aataaataat tgattaggat attttgcaaa
     1441 taaatctttt acaatttgtg cagtgccatc tgggcttcca tcatctataa ccaacacatg
     1501 aaactgttgg tttaaagaaa aaatagcttg taaaatattg gcaatatttt ctttttcgtt
     1561 ataagtaggt ataattacaa ttttttccaa tctggggtat ccattttatt aaaggcacta
     1621 aagtaactaa tcatttttac caaaataaat ttagaggcaa ttactttaca ccctttctat
     1681 tacttagcct ttttttaaca aagtgcggtt taaaatttca gtgaattttt gtttggtatc
     1741 cattgcaaaa tcacctttca tcatccaatc gtaatattgt ggttcttctt taattacttg
     1801 ctctacaggt tttcctttgt gcttgccaaa gttaaaaacc tctacccctt ttactctaat
     1861 aaatcttctt gcaaaatcta taatatcatc ttctccggta aacttaacaa tgctttctat
     1921 tgttgtaccc aattgttcat acctctctac ttgtgcaagt aaaatttccc atgttgccgt
     1981 tgcatcagct tcggcactat gagcatcctc taagttctta tcacaataaa acttataagc
     2041 tgcagttaat gttctttgtt ccattaaatg aaaaactttc tgcacatcta caaacttcct
     2101 tccttgtatt acaaaattaa ttccaactct taaaaattct tctattaata atggcatatc
     2161 aaatctatta ctgttgtacc ctcctatatc acagccttcc ataaattgtt ttacttcatt
     2221 agctacttgt ttaaaagtag gagcgtcttt taccatttca tctgttatac catgaacctg
     2281 tgttgcacca gcagatatag gcatttcagg attaattaat tttcttttta cttgttttgt
     2341 tccatctggt aaaattttca caactgcaat ttctataatt ctatcagaac ttatatttat
     2401 accagttgtt tctaaatcaa taaacgcaat tggattaatt aattgaagtt tcataaatt
//
LOCUS       DAOQHP010000335         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_30893_length_2455_cov_3.342917, whole genome shotgun sequence.
ACCESSION   DAOQHP010000335 DAOQHP010000000
VERSION     DAOQHP010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30893_length_2455_cov_3.342917"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLY81_09415"
     CDS             <1..454
                     /locus_tag="PLY81_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNF30485.1"
                     /translation="YNVGSIFRTADAFLIEGICICGYSPTPEHKQMAKTALGATDTVD
                     WLHYPSAIEAVQTLQQNGYKVFAIEQADESISLENFNSNLYSKMAVVFGNEVEGIQQD
                     VIEACNGCVEIPQLGTKHSLNVATAAGVVLWKLVESYITTSTIHKPNK"
     gene            553..1728
                     /gene="mqnE"
                     /locus_tag="PLY81_09420"
     CDS             553..1728
                     /gene="mqnE"
                     /locus_tag="PLY81_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNF30486.1"
                     /translation="MVDRINSIEVLINKEKNTALQTIAGKVVDNKRLSFDDGVLLFKE
                     ASLPFVGTLANFVRENKHGNKTYFNRNFHIEPTNICVFSCNFCSYSRLYAHREEGWEL
                     SLEDMLKIVKEYDNQPVTEVHIVGGVHPKLTLQFFADTIKAIKTHRPNLHVKGFTPVE
                     LDYMFKKAKLSIEEGMQFLKDAGLESLPGGGAEIFHQDIRSKICNDKVDADGWLKIHK
                     VAHQLGMHSNATMLYGHIEEFWHRVDHMEQLRQLQDETKGFNTFIPLKFRNKNNQMSN
                     VAESSIIEDMRMYAIARLYMDNFSHLKAYWPMLGRQNAQLSLSFGVNDIDGTIDDTTK
                     IYSMAGSEEQSPSMTTEELVQLIKQVKREPIERDTLYNEIKNYSFEQNVLKEELINT"
     gene            1775..2353
                     /locus_tag="PLY81_09425"
     CDS             1775..2353
                     /locus_tag="PLY81_09425"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HNF30487.1"
                     /translation="MKKLFFYGLFIFAVACNSDDKPVIEEKPAAPLSKSKNSDVFNQS
                     FKGLMDTYYHLKDNFITESDTMINTYAKQMQTMVDSLKLSEIKGEAGIVENAKMFTQN
                     ISNDLKNILASKDLLTKRKSFNVLSDNVYNLIKIVQYDREIVYHQHCPMAFEEGDANA
                     YWLSRSSDIKNPFRPTSMLTCGDVADSLDYSK"
BASE COUNT          912 a          345 c          424 g          774 t
ORIGIN      
        1 atataatgta ggaagtattt ttagaacagc agatgcattt ttaatagaag gtatttgcat
       61 ttgtggctat tcgccaacac ccgaacataa gcaaatggct aaaacagctt taggtgctac
      121 agatactgtt gattggcttc actacccatc ggcaatagaa gctgtacaaa ccttacaaca
      181 aaatggctac aaagtttttg ccatagaaca agccgacgaa agtatttctt tagaaaattt
      241 taattctaat ttatattcaa aaatggcagt tgtttttggt aatgaggtag aaggtattca
      301 gcaagatgta atagaggctt gcaatggttg tgttgaaatt ccacaacttg gtactaagca
      361 ctctttaaat gttgcaacag ctgctggagt tgttttatgg aagttggtag aatcatatat
      421 tacaacctct acaatacata aacctaataa atagcaaccc cttcaagtta tttataaact
      481 taattattta atcactttta tctttctaat taatattgta cacttagctt tgctttggat
      541 aaattaaaaa ttatggtgga tagaattaat agtattgaag ttttaataaa taaagaaaaa
      601 aatacagcat tacaaactat tgcaggaaaa gtggtagata ataaaaggtt aagttttgat
      661 gatggtgtat tactttttaa agaagcctcc ttaccgtttg tgggtacatt agctaacttt
      721 gtaagagaga ataaacatgg caacaaaact tattttaacc gcaattttca tatagagcct
      781 acaaatattt gtgtatttag ttgtaatttt tgtagttatt ctcgtttata tgcacataga
      841 gaagaaggat gggagttaag tttagaagac atgttgaaaa tagtaaaaga atacgataat
      901 caacctgtta ccgaagtgca tattgttgga ggtgtacacc caaaattaac tttacaattt
      961 tttgccgata ctattaaagc aattaaaacc caccgtccta atttgcatgt aaaaggcttt
     1021 actcctgtag agcttgatta tatgtttaaa aaagcaaaac tttctataga agaaggaatg
     1081 cagtttttaa aagatgcagg attagagtct ttacctggtg gaggtgcaga aatttttcat
     1141 caggatatcc gatcaaaaat atgcaacgat aaagtagatg ctgatggatg gttgaaaatt
     1201 cataaagtag cccaccaatt aggtatgcat tcaaatgcta ccatgttata tggccatatt
     1261 gaagaatttt ggcatagggt agatcacatg gagcaattac gccaattaca agatgaaact
     1321 aaaggattta atacctttat tcctttaaaa tttaggaata aaaataacca aatgagcaat
     1381 gtggcagaat cttccattat agaagatatg aggatgtatg caattgccag attgtatatg
     1441 gacaacttct ctcatttaaa agcatattgg cctatgcttg gcaggcaaaa tgcacagctt
     1501 tccttaagtt ttggtgtaaa tgatattgat ggaactatag acgataccac taaaatatat
     1561 tccatggcag gtagtgagga gcaaagccca tcaatgacaa cagaagaatt agtgcaactt
     1621 attaaacaag ttaaaagaga acccattgaa agagacacac tctataacga aataaaaaac
     1681 tatagttttg aacaaaatgt tcttaaagaa gaactaataa atacttaaac ttttataaac
     1741 attaaattat tatcttaaaa taaaatacac agttatgaaa aaattatttt tttatgggtt
     1801 atttattttt gctgttgcat gtaacagcga tgataaacca gttattgaag aaaaaccagc
     1861 agcaccatta agtaagagta aaaattctga tgtgtttaat caatcattta aaggattgat
     1921 ggacacttat tatcatttaa aggataattt tattacagag agtgatacaa tgataaatac
     1981 ttatgcaaaa caaatgcaaa caatggtaga tagtcttaag ctgagtgaaa ttaaaggcga
     2041 agccggaatt gtagaaaatg caaaaatgtt tacacaaaat atttctaatg atcttaaaaa
     2101 tatacttgct tctaaagatt tgctaactaa aagaaaatca tttaatgttt tatcagacaa
     2161 tgtttacaat ctaataaaaa ttgttcaata cgatagagaa attgtttacc accaacattg
     2221 cccaatggct tttgaagaag gagatgcaaa tgcttattgg ctaagccgtt cttctgatat
     2281 taaaaaccct tttcgcccta cctctatgct tacttgtgga gatgtggcag attcattaga
     2341 ttattcaaaa taatggaaag gatttgccaa caaaaaaggg ctgcaaattt atttgcagcc
     2401 gacactatcc cataaagtgt gtaagtaaaa taattagggg ttcagctttt ttata
//
LOCUS       DAOQHP010000336         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_31250_length_2438_cov_3.270248, whole genome shotgun sequence.
ACCESSION   DAOQHP010000336 DAOQHP010000000
VERSION     DAOQHP010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31250_length_2438_cov_3.270248"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLY81_09430"
     CDS             complement(<1..281)
                     /locus_tag="PLY81_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF30488.1"
                     /translation="MPLKIYNSLTRKKEEFKPITPGYAGMYVCGPTVSGESHLGHARP
                     YITFDVVYRYLQHLGYKVRYVRNITDAGHFEEEGRAAEDKISTKAILEK"
     gene            complement(465..1220)
                     /gene="fabG"
                     /locus_tag="PLY81_09435"
     CDS             complement(465..1220)
                     /gene="fabG"
                     /locus_tag="PLY81_09435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNF30489.1"
                     /translation="MKLLENKTSIVTGAARGIGAAIAVKLAEHGSNIAFSYVSDSSAA
                     KAAELENQLTALGVKAKAYKSNAGDFAQCETFVNEVMADFGNIDVCVNNAGISKDNLL
                     LRMTAEQWDDVMNINLKSVFNITKLVTRPMMKAKAGSIINMSSIIGIRGNAGQSSYAA
                     SKAGIIGFTKSVALELGSRNIRCNAIAPGFIETDMTHYLKDGDAANDFLKKIPLGRFG
                     KADEIANTTLFLASDMSSYITGQVLSACGGLNI"
     gene            complement(1367..>2438)
                     /locus_tag="PLY81_09440"
     CDS             complement(1367..>2438)
                     /locus_tag="PLY81_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF30490.1"
                     /translation="LIGGSHQILPVNNEASYGDLSAVLFQNTPIWANWLRTPELSIAL
                     MDDWGEKVEAIAKNTIQENVTSISGVPTWTLVLIKRILEITGKQTLKEVWPNLELYLH
                     GGVSFTPYKEQFKHLIGNEIRYLNSYNASEGFFAAQNNFDDEGMLLFLDNGVFYEFLP
                     IEEYGKENPETIGLRDVEIGKQYALVISTNGGLWRYLIGDTIEFTSLNPFKINVSGRL
                     KHFINAFGEEVIVDNTDKAIAQACKKCNAKVNDYTAAPIYFTNNSNGAHEWLIEFEQL
                     PTCLKEFTYELDCALKNINSDYEAKRHLDIALRMPIVQAVDKSVFTNWLKSKNKLGGQ
                     HKVPRLSNDRKYIDEIKQFINN"
BASE COUNT          788 a          433 c          322 g          895 t
ORIGIN      
        1 aatttctcta aaatggcttt agtagaaatt ttatcttctg ccgctctgcc ttcttcttca
       61 aaatgtcctg catctgtaat attccttaca tatcgtactt tgtaccctaa atgctgtaaa
      121 tatctataaa ccacatcaaa agtaatataa ggccttgcat gccctaaatg gctttctcca
      181 cttacagttg gtccacaaac atacatgcct gcataacctg gtgtgattgg tttaaactct
      241 tcttttttac gggtaagtga attataaatt ttgagaggca taaatatttt ttgaaataaa
      301 attttagaat agttaagtat gcaaatgtat tatagtaaaa taaaaaacta ttagcagcaa
      361 taatataata agcaatgtac cataactgta taaatcaaaa ataatgaaaa atagttttca
      421 gtataattga aaactatttt ttaaattgaa aggttagatg gtttttaaat gtttaatcca
      481 ccacatgcac ttaacacttg tcctgtaata taagaactca tatcgctagc aagaaataaa
      541 gttgtattgg caatttcatc tgctttacca aatctgccaa gtggtatttt ctttaaaaaa
      601 tcatttgcag catcaccatc ttttaaataa tgtgtcatat ctgtttcaat aaagcctggt
      661 gcaatggcat tacatctaat atttctacta cccaactcta aagctacact ttttgtaaag
      721 ccaattatac ctgctttact tgctgcatag ctactttggc ctgcatttcc tctaatacca
      781 ataattgaac tcatattaat tatgctacca gccttagcct tcatcattgg gcgtgtaacc
      841 aattttgtaa tattaaaaac gcttttaaga ttgatgttca ttacatcatc ccattgttca
      901 gcagtcattc ttagtaataa attatctttg cttatgcctg cattgtttac acaaacatca
      961 atatttccaa aatctgccat tacctcatta acaaaggttt cgcattgggc aaagtctcct
     1021 gcattgcttt tataggcttt agcttttact cctaatgctg ttaactgatt ttctaattct
     1081 gctgctttgg ctgcactact atcactaaca tagctaaaag caatattact accatgctct
     1141 gcaagtttta ctgcaatggc tgcacctata ccacgagctg ctcctgtaac aatggaggtt
     1201 ttattttcta acaatttcat aagtttactt ttattgggtg gtttttatta ttcaattaaa
     1261 aaataataat taatacaaat taatgaaagt ttttgaatga aaatgaaatt gtagtaaaaa
     1321 agaaaaatga actttgaatt aggagtaatt tttttagaaa agagtgctaa ttattaatga
     1381 attgttttat ttcatcaata tattttctat cgttacttaa ccttggcact ttatgttgcc
     1441 cacctaattt gtttttactt tttaaccagt ttgtaaaaac cgatttatct acagcctgca
     1501 caataggcat tcttaatgca atatctaaat gtcttttagc ttcataatcg ctattaatat
     1561 tttttaatgc acaatctagt tcataagtaa attcttttaa acaagtaggt aactgttcaa
     1621 actcaatcaa ccattcgtgt gcaccattgc tattattagt aaagtaaatt ggtgcagcag
     1681 tataatcatt cacttttgca ttacattttt tacaggcttg tgcaatagct ttatcagtat
     1741 tatctacaat tacttcttcg ccaaaggcat taataaaatg ttttaatctg ccagatacat
     1801 ttattttaaa tgggtttaaa gatgtaaact ctatagtatc accaattaag tacctccata
     1861 aaccaccatt agttgaaatt accaaagcat attgcttgcc aatttctaca tctcttaatc
     1921 caatggtttc ggggttttct ttaccatatt cttcaattgg taaaaattca taaaaaacac
     1981 cattatctaa aaacaataac attccttcgt catcaaaatt attttgagca gcaaaaaagc
     2041 cttcgctggc attgtaagaa ttgaggtaac gaatttcgtt tcctattaaa tgtttaaact
     2101 gttctttata aggtgtaaat gaaactcccc catgcaagta caactctaaa tttggccaaa
     2161 cttctttaag tgtttgttta cctgtaattt ctaaaattct tttaataagt accaatgtcc
     2221 atgttggaac acctgatata gaagtaacat tttcttgaat ggtatttttt gctatagctt
     2281 ctactttttc gccccaatca tccattaaag caatggatag ttctggtgtt cttaaccagt
     2341 ttgcccaaat gggtgtgttt tgaaaaagta ctgcacttaa atcgccataa gatgcttcat
     2401 tatttactgg taaaatttga tggcttcctc caattaat
//
LOCUS       DAOQHP010000337         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_31328_length_2434_cov_3.234973, whole genome shotgun sequence.
ACCESSION   DAOQHP010000337 DAOQHP010000000
VERSION     DAOQHP010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31328_length_2434_cov_3.234973"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..777)
                     /locus_tag="PLY81_09445"
     CDS             complement(37..777)
                     /locus_tag="PLY81_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF30491.1"
                     /translation="MNFEVYSVLFFGLLFIVAFLYASVGHGGASGYLALMAFFSFAPE
                     VMRPTALLLNIFVSFTAFIQYYRGNHFKWKLFWPFAIASFPAAFLGGLIVIDVKLYKL
                     ILGVLLLFCVIRLIGIKFNNNNKPKEQSLILSLIIGAAIGLFSGMIGIGGGIILSPVI
                     LLLHWGNMKQTAAVSALFILVNSISGMAGLLTKGIDVSNNMIVMIAIAFAGGLLGSYV
                     GAIKATNSFLKTLLATVLLLASYKLLFT"
     gene            complement(793..1386)
                     /locus_tag="PLY81_09450"
     CDS             complement(793..1386)
                     /locus_tag="PLY81_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNF30492.1"
                     /translation="MKPLSIKIIIALYIIIASCNSATSSKSEEKLANKDSADLCLKPV
                     AASDTAKFISKQIIIKGDVEREVILTVDSLKKMQTKILNNLNVVCQTGITTFKHKTKT
                     GVLLKDIIDKAGIKKTNHKDRNFVIVARATDNYKATFSWAEIYNNPTGENTYVLFEED
                     GQPIKTHGDMKLICANDIKTGPRQVIWLKSIEINRIN"
     gene            complement(1491..>2434)
                     /locus_tag="PLY81_09455"
     CDS             complement(1491..>2434)
                     /locus_tag="PLY81_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNF30493.1"
                     /translation="YDGTINKAEAVRIFTGAALPFNANTVVIQEHVEKKGNAIKILDT
                     NLKNGSNVRPIGSQTKQNELLLEKGHLLTPASIALLANFGIKTISVFSNPTISIITTG
                     KELLQLNEELVEGKVYESNSFALAAVLKQMKITPTSIEMVDDDEGLIINAIKNTKNAD
                     ILILTGGVSVGDYDFVNSALEKCGALKIFHKVKQKPGKPFYFGKINHTLVFGLPGNPA
                     SVLSCFYMYVAEAVGYYTQKKYFKNATVTLAHHFQKKAGLTFFLKGKTDNNDVIILDN
                     QESYKMNSFALANCLIELNPEKEFFEKGELVHIKLIV"
BASE COUNT          823 a          410 c          312 g          889 t
ORIGIN      
        1 cacaaatgca tctatatgtt taaatttttc cataaactac gtaaacaata atttatatga
       61 agccagtaat aacactgtag ctaataaagt ttttaaaaac gagtttgttg ctttaatagc
      121 accaacataa gagccaagta gtccgccagc aaatgctatt gcaatcatta ctatcatatt
      181 gttgctaaca tcaattcctt tggttagtaa gcctgccata ccagaaatag aattaactaa
      241 aataaataag gccgataccg ctgctgtttg tttcatattg ccccaatgaa gtaataaaat
      301 aacaggactt aaaataatac caccaccaat accaatcata ccagaaaata aaccaattgc
      361 agcaccaata attaatgata aaataaggct ttgttcttta ggtttattat tattattaaa
      421 ctttatgcct attaatctaa taacacagaa taacaacaaa acaccaagaa ttaatttata
      481 taattttaca tcaataacaa ttaggcctcc taaaaaagca gcaggaaaag atgcaatagc
      541 aaagggccaa aataatttcc atttaaaatg attgcctcta tagtattgta taaaagctgt
      601 aaacgaaaca aaaatattta gtagcaaagc agttggcctc attacttctg gagcaaatga
      661 aaaaaatgcc attaatgcta aataaccact agcaccgcca tgccctactg atgcatataa
      721 aaatgcaaca ataaatagta agccaaaaaa caaaacgcta tatacttcaa aattcattgt
      781 ataaattagc aattagttta ttctatttat ttcaattgat tttagccaaa taacttgtct
      841 tggtccagtt ttaatatcat tggcacaaat tagcttcata tcgccatgtg tttttattgg
      901 ctgtccatct tcttcaaata atacatacgt attttctcct gttgggttat tataaatttc
      961 tgcccacgaa aatgttgctt tataattatc tgttgccctt gctactataa caaaatttct
     1021 atctttatga ttggtttttt taatgcctgc cttatcaatt atatctttta ataatacccc
     1081 tgtttttgtt ttatgtttaa atgttgtaat accggtttgg catacaacat ttaaattatt
     1141 taatattttg gtttgcattt tttttaagga atcaacagtt aatattacct ctctttccac
     1201 atctccttta attataattt gcttactaat aaacttggct gtatcacttg ctgcaacagg
     1261 ctttaaacat aaatctgccg aatctttatt tgctaatttt tcttctgatt tactacttgt
     1321 tgcactattg caacttgcta ttattatata cagtgcaata attattttga tagataaagg
     1381 tttcatattt tgctgaatta ttaatagtgt gcattttaga acaatagttt atgctgcaag
     1441 atttaagacc tcaaaataaa aagttagaat gtaaaaaaat atgactagaa ttatacaatc
     1501 agttttatat gaactaattc tcctttttca aaaaattctt tctccggatt tagttcaatt
     1561 aaacaatttg ctagtgcaaa agagttcatt ttatagcttt cctgattatc taaaataata
     1621 acatcgttgt tatctgtttt tccttttaaa aaaaaggtta gccctgcttt tttttgaaaa
     1681 tgatgagcta atgttactgt tgcatttttg aaatattttt tttgtgtata atagccaaca
     1741 gcttcggcca catacatata aaagcaagac aataccgatg ctggattgcc tggcaaacca
     1801 aatactagcg tatggttaat ttttccaaaa taaaatggct ttccgggctt ttgttttact
     1861 ttatgaaata tttttagtgc accacatttt tctaaagctg aatttacaaa atcataatct
     1921 ccaacagata cacctcctgt aaggattaaa atatcagcat tttttgtatt cttgatagca
     1981 ttaattatta gcccttcatc atcatcaacc atttctattg atgttggtgt aattttcatt
     2041 tgttttaata cagcagctaa tgcaaacgaa tttgattcgt aaacttttcc ttcaaccaat
     2101 tcttcattaa gttgtaaaag ttctttgcct gttgttatta tgcttatagt ggggtttgaa
     2161 aatactgaaa tagtttttat tccaaagttt gccaacaagg caatagaagc tggtgtaagc
     2221 aagtgacctt tttctaacaa taattcattt tgtttagttt gagagccaat tggccttaca
     2281 ttactgccat tttttaaatt agtatccaaa atttttatag cattcccttt tttttctaca
     2341 tgctcttgta ttacaacagt attggcatta aatggcaatg cagcaccagt aaaaattctt
     2401 acagcttctg ccttattaat tgtgccatca taac
//
LOCUS       DAOQHP010000338         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_31460_length_2427_cov_3.339798, whole genome shotgun sequence.
ACCESSION   DAOQHP010000338 DAOQHP010000000
VERSION     DAOQHP010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31460_length_2427_cov_3.339798"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLY81_09460"
     CDS             complement(<1..939)
                     /locus_tag="PLY81_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNF30494.1"
                     /translation="MNQTAAPTSGHPKGLYLLFFTEMWERFSYYGMRAIFVLYMTKML
                     LMPDDTASNIYGSYTGLVYLTPLLGGYLSDRFLGNRRSIEIGGVLMALGQFLMFFSAS
                     ATGTMAITFMWAGLTALIIGNGFFKPNISTMVGQLYPAGDRRVDSAFTIFYMGINLGA
                     FFSPLVCGTLAEKIDFKWGFFAAGIGMVIGLLLFMALKNKYLNTEDGKPIGKSVKPLD
                     TKNIFYLVGSIALIFFLLNFKTLFNNNWDVIGWVIYGSMIAIPVIILSDKTITPVERS
                     RILVIFILAFFVIFFWGAFEQAGASLTIFADRQTDRH"
     gene            1100..2386
                     /gene="purD"
                     /locus_tag="PLY81_09465"
     CDS             1100..2386
                     /gene="purD"
                     /locus_tag="PLY81_09465"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNF30495.1"
                     /translation="MNILLLGSGGRENALAWKMKQSHHCNNLFIAPGNAGTNLIGINV
                     SIDVNDFESQKQFCIEKNIDIVVVGPEEPLVNGVFDYYKKDELLQHIIVVGPSAKAAQ
                     LEGSKAFSKQFMQTYKIPTATYAEFSVSEIEEAKKYIQQHSLPIVVKADGLAAGKGVI
                     ICETTEHAISVVEDMLIHKQFGKASDKVVVEEFLNGIELSVFALTNGKDYKIIGHAKD
                     YKRVGEGDTGLNTGGMGCVSPVAFADEIFLQKVEETIIKPTISGLEKEELIYNGFVFF
                     GLMNCNGTPYVIEYNCRLGDPETEVILPRLETDLVSLFAAMGNNTLADANIEFFEESF
                     ATIMAVSGGYPSTYKTNYIIHNLNNLKTEKDILIFHAGTKQNENGEIVTNGGRVLAVT
                     AKGSSIKNAVEKSIHVLNSISFEGMYFRRDIGYEFK"
BASE COUNT          901 a          344 c          421 g          761 t
ORIGIN      
        1 atgtctatct gtttgtctgt ctgcaaaaat ggttaacgat gcacctgcct gctcaaaagc
       61 accccaaaag aaaattacaa aaaatgccag aataaaaatt actaaaattc tacttctttc
      121 taccggtgta atagttttat cagataatat aattacaggt atggcaatca tagagccata
      181 tattacccag ccaattacat cccaattatt attaaaaaga gttttaaaat ttaagagaaa
      241 aaatattaaa gcaatggagc caactaggta aaaaatattt ttagtatcta atggttttac
      301 agattttcca attggttttc catcttcggt attaaggtat ttatttttta aagccataaa
      361 taatagcaag ccaataacca ttcctatacc agctgcaaaa aatccccatt taaaatctat
      421 tttttctgct aaagtaccac atactaaagg agagaagaaa gcacccaaat taatacccat
      481 ataaaaaatt gtgaaagcac tatcaactct tctatctcct gctgggtaca attggccaac
      541 cattgttgaa atattgggtt taaaaaaacc attcccaatt attagagctg ttaagccagc
      601 ccacataaat gtaattgcca ttgttccggt tgccgaggca ctaaaaaaca ttaaaaattg
      661 ccctaatgcc atcaatactc ctccaatttc aatacttctt ctattaccta aaaaacggtc
      721 gcttaaatag ccacctaata aaggagttaa atacactaaa ccggtataac taccataaat
      781 atttgaggca gtatcatcgg gcattaaaag cattttggtc atatataaca caaaaattgc
      841 tctcatgcca taataactaa aacgttccca catttcagta aaaaataata aataaagacc
      901 cttaggatgt cctgaagtag gtgcagcagt ttgattcata tacaattaat tttcgttaga
      961 ggttattttg aaggcttgaa aataagtaga aaagttaata aacttcttaa aaagttttaa
     1021 cagttaaagt agaggaataa aaattcaatg ttttgtttaa aaaatcgcat ttactttcgc
     1081 cttaaataat tatgtaaata tgaatattct tttgttaggt agtggtggaa gagaaaatgc
     1141 tttggcatgg aagatgaagc aaagccatca ttgtaataat ttatttatag caccaggtaa
     1201 tgctggtacc aatttaatag gaataaatgt atctattgat gtaaatgatt ttgaaagtca
     1261 aaagcaattt tgcatagaaa aaaatataga tattgttgtt gtaggtccag aagagccttt
     1321 ggtaaatggt gtgtttgatt attacaagaa agatgagttg ctacaacata taattgtagt
     1381 tggtccatct gctaaagctg cacagttaga aggtagtaaa gcatttagta aacaatttat
     1441 gcaaacctat aaaattccta cagctactta tgcagagttt tctgttagtg aaatagagga
     1501 agcaaaaaaa tatatacaac aacatagttt acccattgtt gttaaggcag atggtttggc
     1561 tgcaggtaaa ggagtaataa tttgcgaaac taccgaacat gctattagtg ttgtagaaga
     1621 tatgcttata cacaaacaat ttggtaaagc aagcgataaa gtggtagttg aggagtttct
     1681 taacggaata gaattaagtg tttttgcttt aacaaatgga aaagattata aaattattgg
     1741 ccatgctaaa gattataaaa gagtaggaga aggagatact ggtttaaaca caggtggcat
     1801 gggttgtgtt agtcctgttg cttttgcaga tgaaattttt ttacaaaaag ttgaagaaac
     1861 cattattaaa ccaacaatta gtgggttaga aaaagaagag cttatttata acgggtttgt
     1921 attttttgga ttgatgaact gtaatggcac tccttatgtt atagaataca attgtagatt
     1981 aggcgaccct gaaactgaag taatattacc aaggttggaa acagatttag taagcctttt
     2041 tgcagcaatg ggtaacaata ctttggcaga tgccaatatt gaattttttg aggaaagctt
     2101 tgcaacaatt atggctgtta gtggtggcta tccttctacc tataaaacaa attacatcat
     2161 tcataactta aataatttaa aaactgagaa ggatatttta atttttcatg caggcactaa
     2221 acaaaatgaa aatggagaga ttgtaactaa tggaggaaga gtgcttgctg taactgcaaa
     2281 agggagtagt ataaaaaatg ctgtagaaaa atctatccat gttttaaatt caatatcttt
     2341 tgaaggtatg tattttagaa gagatattgg ctacgagttt aagtaaataa tgctactagt
     2401 attgaaaatt ttaaaggctg caatatt
//
LOCUS       DAOQHP010000339         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_31598_length_2421_cov_5.235841, whole genome shotgun sequence.
ACCESSION   DAOQHP010000339 DAOQHP010000000
VERSION     DAOQHP010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31598_length_2421_cov_5.235841"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..517)
                     /locus_tag="PLY81_09470"
     CDS             complement(17..517)
                     /locus_tag="PLY81_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF30496.1"
                     /translation="MSAEIKVAIADDHLIFRKGVIMCLRPYSNIKFVLEASNGEELLK
                     NIEEANPNVVLMDLRMPVKDGIEATKYINKHYPKIAVLILTMHDEEKFVTHLMENGAN
                     GYLLKNADPLEIKKAILEVATKGYYLNNYVNRILLKRAHNKSKSITINANPDEISEKE
                     KNQFNK"
     gene            complement(589..1119)
                     /gene="purE"
                     /locus_tag="PLY81_09475"
     CDS             complement(589..1119)
                     /gene="purE"
                     /locus_tag="PLY81_09475"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNF30497.1"
                     /translation="MQNKQPTIISIVMGSDSDLTVMQAAASFLQQLNIAFELTIVSAH
                     RTPQRLMSYAATANQRGIQVIIAGAGGAAHLPGMIASITTLPVIGVPIKSSNSIDGWD
                     SILSILQMPNGVPVATVALNAAKNAAILAAQIIALQNKNIAEALEHFKQTMQAEVENK
                     ITETAKIWNNEFDKRK"
     gene            complement(1199..1813)
                     /locus_tag="PLY81_09480"
     CDS             complement(1199..1813)
                     /locus_tag="PLY81_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNF30498.1"
                     /translation="MLQKNYYKILQVSPQCTQEEIKKAYRKLALQFHPDINHKTTQIF
                     NEIKEAYEVLGNATSRSKYNTTFFYYLVEDKNVNIHSIVQQTYQLTSFIQSGNRFTMD
                     YEMIATYIHHILQPLQVNLIQTTAEKDKYQQIENNLYLTAAALPFHQYQKVYTLLSNV
                     FPNKINLYNKLLKQKKQNWLGEKYAILIAITMTIMLCFIIAYLS"
     gene            complement(1852..>2421)
                     /locus_tag="PLY81_09485"
     CDS             complement(1852..>2421)
                     /locus_tag="PLY81_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30499.1"
                     /translation="YIYKYFILNKKLSLPGIGCLIIENCKATFNDNKNAILPPNPTIN
                     FSTTTTLADKSFYHFLATELHIEEVDAIKNFHDFSYQLQNGINKANGIVLPGIGTIKK
                     HPSGSLEFETEVDANQYLSEINLSGNKKKTTAAIVAEQQHIFTPPIIDEVNSEDGNNS
                     TQKQTKDYWWIFAILLALIGIVAIYYKLF"
BASE COUNT          743 a          329 c          356 g          993 t
ORIGIN      
        1 ttttatttta ttttttttat ttattgaact ggtttttttc tttttcagaa atttcatcgg
       61 ggttggcatt aattgttata ctttttgatt tattgtgagc tctttttaat aatattctgt
      121 ttacataatt atttaaataa tagcctttag tggcaacttc taaaattgct ttttttattt
      181 ctaatgggtc ggcatttttt agtaaataac cattagctcc attttccatt aagtgtgtta
      241 caaacttttc ttcatcgtgc atggttaaaa tgagcactgc tattttaggg taatgtttgt
      301 ttatatattt tgttgcttca ataccatcct taactggcat tcttaaatcc attaaaacta
      361 catttggatt ggcttcttct atatttttta gtaattcctc accgttactt gcttctaata
      421 caaacttaat attactataa ggtcttaaac acataataac tccttttcta aaaattaggt
      481 ggtcatctgc aatggctact tttatttctg ctgacatagt tttgctaagt taagtaagaa
      541 tatttctatt gaattgtaac taatgaaaag aatttatgag ttactaattt attttctttt
      601 atcaaattcg ttattccaaa ttttagcagt ttcagtaatt ttattttcta cttctgcttg
      661 catagtttgt ttaaaatgtt ctaaagcttc tgcaatattt ttattttgta gtgcaattat
      721 ttgggctgct aaaatagctg cattttttgc agcatttaga gctacagttg caactggtac
      781 accattaggc atttgaagta tggataggat agaatcccat ccatctatag agtttgagct
      841 ttttataggc acaccaatta caggtaaagt ggtaatactt gcaatcatac caggtaaatg
      901 ggctgcacca cctgcacctg caataattac ttgtatgcct ctttgatttg ctgttgcagc
      961 ataactcatt aaacgttgcg gcgttctatg agccgaaaca atagttaatt caaaagctat
     1021 gtttaattgt tgtaaaaaac ttgctgcagc ttgcattaca gttaagtcgc tatcgctacc
     1081 cataactata cttattattg ttggttgttt gttttgcatt gattgaacta gatttaaaac
     1141 ttaaaatata aatagtttac ttatattgaa ttaaccaaaa ttaattatta aataacaatt
     1201 aagataaata tgcaataata aaacataaca taatcgtcat tgtaattgca atgagtatgg
     1261 catatttctc tcctaaccag ttttgttttt tttgttttaa tagtttatta tacaggttta
     1321 ttttattagg aaatacatta cttaataaag tatatacttt ttgatattga tgaaatggta
     1381 gagctgctgc tgtaagatac agattgtttt ctatttgttg gtacttgtct ttttctgctg
     1441 tggtttgaat taaattaacc tgtaatggtt gtaaaatatg atgaatataa gtggcaatca
     1501 tttcataatc catagtaaat ctattaccag attgtatgaa gcttgttagc tgatatgttt
     1561 gctgtactat ggaatggatg tttacatttt tatcttctac caaataataa aaaaaggtag
     1621 tattgtattt tgacctactt gttgcattac ctaaaacttc ataagcttct tttatttcgt
     1681 taaaaatttg tgttgtttta tggttaatat caggatgaaa ctgtagtgcc agttttctat
     1741 atgctttttt tatttcttcc tgggtgcatt gaggcgatac ttgaagaatt ttataataat
     1801 tcttttgtag catttttaat tactctacaa tttaataaaa tagattagta cctaaaataa
     1861 tttatagtaa atagctacaa tacctattaa agctagtaaa atagcaaata tccaccaata
     1921 gtctttggtt tgtttttgtg tactgttatt accatcttca gaattaacct catcaataat
     1981 tggaggtgtg aaaatatgtt gttgctctgc tacaattgca gcagttgttt tctttttatt
     2041 accagaaaga tttatttcgg ataaatactg attggcgtcc acctcagttt caaattctaa
     2101 actgccagag gggtgttttt ttattgtgcc aataccaggt aaaactatac cattagcttt
     2161 attaatacca ttttgtaatt gataagaaaa atcgtgaaaa ttttttattg catctacttc
     2221 ttctatatgt aattcagttg ctaaaaaatg gtaaaaagat ttatcggcta atgttgttgt
     2281 tgttgaaaaa ttaattgtag gattgggagg taaaatagca tttttattgt cattaaaagt
     2341 tgctttgcaa ttttcaataa ttaaacaccc aatacctggt aagcttaatt ttttatttaa
     2401 aataaaatat ttataaatat a
//
LOCUS       DAOQHP010000340         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_31810_length_2412_cov_4.623674, whole genome shotgun sequence.
ACCESSION   DAOQHP010000340 DAOQHP010000000
VERSION     DAOQHP010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31810_length_2412_cov_4.623674"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLY81_09490"
     CDS             <1..1042
                     /locus_tag="PLY81_09490"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNF30500.1"
                     /translation="FIIQDSIKISYNGNTRFVQLQAYGQNAIFLKNKLVTIDTTWNKD
                     LPIVVVGALTVQPSVTLTIAKGTKVYFHANAPMLVQGTLKAIGEKDEPSKIIFTGDRL
                     DEPYRDFPGGWPGIYFLDNSVNNVMQYCIIKNAYQGTIAQNPSSNANPKLILNECILD
                     NIYDVAVGATNSSIEANNCLISNSGYNIYLTAGGNYNFTHCTVTSIGNYYLQHKYPVL
                     NISNKVSETISNDLTANFTNCIVYGDGGIPEDEISIKKQGSTIFNVNFNNTLYKLKNN
                     EPSTANFNGNKLKNIPPDFDSININNRYYNFRLKPNAAAINKGTNTSLLTDLDGNNRS
                     IGLPDLGCYERQ"
     gene            1213..1755
                     /locus_tag="PLY81_09495"
     CDS             1213..1755
                     /locus_tag="PLY81_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNF30501.1"
                     /translation="MRAKHYLILLVLILTAASFTIYKQKKIYSFKVDGIEGGKINFAK
                     FKGKKILIVNTASECQYTYQYAELQSLYTTYQDKLVIVGFPCNQFGHQEMGSNDDINS
                     FCKVRYGVSFPLASKVDVKGENAAPIYKWLCNKSENGVMDVTIKWNFNKFLIDEKGRL
                     LAHFPSNVKADSDEILKYLK"
     gene            complement(1831..>2412)
                     /locus_tag="PLY81_09500"
     CDS             complement(1831..>2412)
                     /locus_tag="PLY81_09500"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNF30502.1"
                     /translation="LAPIGITVWVIVSLFNVIDDLLPNLLSNVFPNYIKKDINGNFEK
                     ITGLGFVMVIILVLFVGWLSSLLQIGKALNIFDKVLENTPGIKIIYSSVKDFFEAFAG
                     NKKKFDKPVLVNVDANNVWRIGFITKSDATDFDLKDHLVVYVPHSYAISGITYFVPKS
                     NVKELKNITATNAMKFTVSGGVVDFDDVNDKAN"
BASE COUNT          937 a          349 c          324 g          802 t
ORIGIN      
        1 atttattatt caagatagta ttaaaataag ctataatggt aatacccgtt ttgttcagtt
       61 acaagcttat ggccaaaatg ctattttctt aaaaaataaa ttggtaacta ttgataccac
      121 ttggaataaa gatttaccca ttgttgtagt tggtgcttta actgttcaac catctgtaac
      181 attaaccata gcaaagggaa ccaaagtata ttttcatgcc aatgctccaa tgttagtaca
      241 aggtacgctt aaagcaattg gagaaaagga cgagccttca aaaattattt ttacaggaga
      301 tagattagat gaaccttata gagattttcc tggtggctgg cctggcatct attttttaga
      361 taacagtgta aataatgtca tgcaatattg tattattaaa aatgcttacc aaggtacaat
      421 tgctcaaaac ccatcatcta atgcaaaccc aaaactaatt ttaaacgaat gtattttaga
      481 taatatttat gatgttgctg tgggtgctac caactcatcc attgaagcaa ataattgttt
      541 aattagtaat agtggttata atatatattt aacagcaggc ggcaattata attttacgca
      601 ttgtacagtt acaagtattg gtaattatta tttacaacat aaatatccag tactaaatat
      661 cagtaataaa gtatctgaaa caatcagtaa tgatttaaca gccaatttta ccaattgtat
      721 tgtttatggc gatggcggaa taccagaaga tgaaatttct attaaaaaac aaggcagcac
      781 catttttaat gtaaacttca ataacacttt atataagtta aaaaataacg agccaagtac
      841 tgctaatttt aatggtaata aattaaaaaa tataccacca gattttgata gtattaatat
      901 aaataataga tattataatt ttagattaaa accaaatgct gctgctataa ataaaggcac
      961 taatacaagt ttactaacag acttagatgg aaataataga agtattggtt taccagattt
     1021 aggttgttac gaaagacaat aattgtttat taattttttt taagccgaaa aaaataccta
     1081 cttatcttat tttaaatcaa tgtattacat acatttattt tatttaggta ttacctcaaa
     1141 ctgtcatttt tcctaacaaa attatattat tcaatatttt ttttaacttt actacaccaa
     1201 aatcaagagc ttatgagagc aaaacattac ctcatattac ttgtattaat tttaactgct
     1261 gcatctttta caatttacaa acaaaaaaaa atttactctt ttaaagtgga tggcattgaa
     1321 gggggaaaaa taaattttgc taagtttaaa gggaaaaaaa tattgattgt aaatacagct
     1381 tcagaatgtc aatacactta tcaatatgcc gagttgcaat ctttatatac aacctatcaa
     1441 gacaaattag tgattgttgg atttccttgc aatcaatttg gacatcaaga aatgggtagc
     1501 aatgatgata ttaactcttt ttgcaaggta agatatggtg taagttttcc tttagcaagc
     1561 aaggtagatg taaaaggaga aaatgcagca cccatttata aatggctttg caataaaagc
     1621 gaaaatggtg ttatggatgt aactataaaa tggaacttta ataagttttt aatcgatgaa
     1681 aaaggaagat tattagcaca ttttccgagt aatgtaaaag cagatagcga tgaaatttta
     1741 aaatatttaa aataaatatt aaaaggtaac atgcaaaatt tactattcaa aaacaatcaa
     1801 acatttagct tagacaagta aaaaataagt ttagtttgcc ttatcgttaa catcatcaaa
     1861 atctacaaca ccaccactta ccgtaaattt cattgcattt gtagcagtta tattctttaa
     1921 ttctttcaca ttacttttag gtacaaaata agtaatacca cttattgcat aagaatgtgg
     1981 cacatatacc actaaatgat ctttcaaatc aaaatcagta gcatcagatt tggtaataaa
     2041 cccaatacgc caaacattat ttgcatctac atttactaat accggcttat caaatttctt
     2101 cttattacct gcaaaagctt caaaaaaatc tttaacggaa ctataaataa ttttaatacc
     2161 tggtgtattt tctaaaactt tatcaaaaat atttaatgct ttaccaattt gtaataaaga
     2221 agagagccaa ccaacaaaaa gtacaagtat aataaccatt acaaacccta atcctgtaat
     2281 tttttcaaaa ttaccattga tatctttttt aatataatta ggaaaaacat tactcaataa
     2341 gttgggcaac aaatcgtcta ttacattaaa aagagaaaca attacccaaa cggtaatacc
     2401 aattggtgct aa
//
LOCUS       DAOQHP010000341         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32092_length_2400_cov_4.054158, whole genome shotgun sequence.
ACCESSION   DAOQHP010000341 DAOQHP010000000
VERSION     DAOQHP010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32092_length_2400_cov_4.054158"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2400
                     /locus_tag="PLY81_09505"
     CDS             <1..>2400
                     /locus_tag="PLY81_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNF30503.1"
                     /translation="NLVNNSYEIACRAKLPIADVCISENETSSPLFFQYHHQFYAWQK
                     AEDIEIIERFLPTGKIYIDADEWSKNLQQLILPLTKLYQVNFGNLHKTEVKDEKPQLK
                     LFLKEKGEYLVFEPVFTYKGYDVSNTDKETIILPMAGKLLIVYRNIEAEKTFLQNIEN
                     LHSHFIKPIDSFTLALRGADVLKNNWFFLFIDAMKDLNVQVFGFELLKNFRFNTAKPS
                     TKIYISSNTDWFDAKIEIQFGEQKVTVDDVKKALANKQQFVPLNDGTLGILPEEWIRK
                     YSLLFKVGEGKTNNMKLSKYQFSVVEDLYNQRDEEELFIQLEEKYDTLRYNHEIKKIK
                     APTHLKSILRPYQESGFQWLNYLSEVKWGGILADDMGLGKTIQALSFLQHLKEVNKSI
                     KALVVCPTTLMFNWQNEIKKFTPALTYFIHHGGARLKSNLSNVAIDVIITTYGTLRSD
                     IKYFAEVEFDYVILDESQAIKNPASKVTKAACVLKAKNKLCLTGTPLQNNTFDIFAQM
                     NFLNPGMLGSQEFFKNEFAVPIDKLGEKEQKDHLRKLLYPFILRRTKEQVAKDLPEKQ
                     EMILYCEMGKEQRKIYDAYRNDFRDKILGVVQEKGIQKSQLTILQGLMKLRQICDSPS
                     ILKEKQGEVFPNESVKLEELAREITENISNHKALIFSQFIGMLSLIKENMKANGIIYE
                     YFDGTTSAAERERAINRFQNDDECRVFLISLKAGGVGLNLTAADYVYIVDPWWNPAVE
                     QQAIDRTHRIGQTKNIFAYRMICIDTIEDKILQLQERKRILAKELIADDASFVKALTK
                     A"
BASE COUNT          924 a          324 c          417 g          735 t
ORIGIN      
        1 aaatcttgta aacaacagtt acgaaattgc ctgtagagcc aaattaccaa ttgccgatgt
       61 ttgcattagc gaaaatgaaa cttcctctcc tttgttcttt caatatcatc atcaatttta
      121 tgcatggcaa aaagcagaag atattgagat tatagaaaga tttttaccca ctggtaaaat
      181 ttatattgat gcagatgaat ggagcaaaaa tttacaacaa ttaatattgc ctcttacaaa
      241 attatatcag gtaaattttg gcaacttaca taaaacagag gtaaaagacg aaaagccaca
      301 actaaaactt ttcttaaaag agaaaggtga atatttggta tttgaaccag tttttactta
      361 caaaggttat gatgtaagta ataccgataa agaaacaatt attttaccaa tggctggcaa
      421 acttttaata gtttacagaa atattgaggc agaaaaaacc tttttacaaa acatagaaaa
      481 tcttcactct cattttataa aacctattga tagttttacc cttgcattgc gtggtgcaga
      541 tgttttaaaa aataattggt tctttttgtt tatagatgca atgaaggatt taaatgtgca
      601 ggtatttggg tttgagttgc ttaaaaattt taggtttaat actgcaaagc catctaccaa
      661 aatttatata agcagcaata cagattggtt tgatgcaaaa attgaaatac aatttggtga
      721 acaaaaagta actgttgatg atgttaaaaa agctttggcc aacaagcaac aatttgtacc
      781 acttaatgat ggcactttag gtattttacc agaagaatgg ataagaaaat actctttact
      841 atttaaagtt ggcgaaggta aaaccaacaa catgaaattg agtaaatatc aatttagcgt
      901 tgttgaagat ttatacaatc aacgagacga agaagaatta tttatacagt tagaagaaaa
      961 atacgatacc cttaggtata accacgaaat aaaaaaaatt aaagctccta ctcatctaaa
     1021 atcaatatta aggccatatc aagaaagtgg ttttcagtgg ttaaattatt taagcgaagt
     1081 aaaatggggt ggaattttag ctgatgatat gggcttgggt aaaaccattc aagctctaag
     1141 ttttttacaa catttaaaag aagtaaataa gtctataaaa gcattggttg tttgccctac
     1201 tacactaatg tttaactggc aaaacgaaat aaaaaaattt acacctgcac ttacttattt
     1261 tattcatcat ggcggagcca gattaaaatc taatttatct aatgttgcta tagatgttat
     1321 tattaccact tatggtactt taagaagtga tataaaatat tttgcagaag ttgaatttga
     1381 ctatgtaata ttagatgaaa gccaagcaat taaaaatcct gctagtaaag taaccaaagc
     1441 agcatgtgtt ttaaaagcaa aaaataagtt atgcctaacg ggtacccctt tgcaaaacaa
     1501 cacatttgat atttttgcac aaatgaattt tttaaaccca ggcatgctag gaagccagga
     1561 attttttaaa aatgaatttg cagtacctat tgataaatta ggagaaaaag agcaaaaaga
     1621 ccatttaaga aaattattgt acccttttat tttaagacga acaaaagaac aagtagcaaa
     1681 agatttgccc gaaaaacaag aaatgatttt gtattgtgag atgggtaaag agcaaagaaa
     1741 aatttatgat gcttaccgaa atgattttag agataaaatt ttaggggttg ttcaagaaaa
     1801 aggaatacaa aaaagccagc tcactatttt gcaaggctta atgaagttaa ggcaaatttg
     1861 cgatagccct agtatattaa aagaaaaaca aggagaagtt tttcctaatg aatcggtaaa
     1921 attagaagag cttgcaagag agattactga aaacataagt aaccataaag ctcttatttt
     1981 ttctcagttt attggtatgt tgtctcttat taaagagaat atgaaagcca atggtattat
     2041 ctatgaatat tttgatggaa ctactagtgc tgcagaaaga gaaagggcaa ttaatcgttt
     2101 tcaaaacgat gacgaatgta gagtgttttt aatttcacta aaagctggtg gtgttggttt
     2161 aaatttaact gctgcagatt atgtatatat tgttgatcct tggtggaacc ctgcagtaga
     2221 gcaacaagcg attgatcgta cacaccggat cggacaaaca aaaaatattt ttgcctatcg
     2281 tatgatatgt attgatacaa tcgaagataa aatattgcaa ttacaagagc gcaaacgtat
     2341 attggcaaaa gaattaattg ccgacgatgc tagctttgta aaagcattaa ctaaagcaga
//
LOCUS       DAOQHP010000342         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32132_length_2398_cov_4.772087, whole genome shotgun sequence.
ACCESSION   DAOQHP010000342 DAOQHP010000000
VERSION     DAOQHP010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32132_length_2398_cov_4.772087"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLY81_09510"
     CDS             <1..212
                     /locus_tag="PLY81_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HNF30504.1"
                     /translation="IPGIGEVARSFGQELTPYSMLSRSLGGLKGNTLIIALPGSTKGA
                     SESMHALFPYILHLFKVMDYFRHEG"
     gene            complement(215..2275)
                     /locus_tag="PLY81_09515"
     CDS             complement(215..2275)
                     /locus_tag="PLY81_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNF30505.1"
                     /translation="MIVSQLIHPKSIAVIGASDNTTKPGGMVLQNLLKGNFNGPIYAI
                     NPKPLNIEGVISYDNINKINTVDLAIIAIPASLCLQTIKELLKKGAKAFIIFSAGFSE
                     AGEQGIAIEKEIVEIINKSNATLIGPNCVGVINQHYKGVFTTPVPEYDPNGCELISSS
                     GATAVFIMESALLTGLRFSNIYSIGNAAQTGVEEILEYLDNTFDEKKSSKVKLLYLEQ
                     VKKPFKFLKHATSLIEKGCRIAAIKSGYSEAGGRAASSHTGALATSDNIIRALFRKAG
                     IVYCSSRDELIAVGSIFQSKKLQGENIAIITHAGGSAVMLTDALTSNGLNVPPFESDR
                     TQELLAKLNPGSSVNNPIDFLATGTAKQLGEIIDFCDNYEAVDAMVVVFGSPGLFNVK
                     DVYELLDEKMNSCKKPIYPVLPSLINAEKEIKQFLNKGRVNFSDEVNLGKALAHVYVS
                     TKYKFEKTILPDMDIVSIRSIINTAQNGYLDAATCVELIEAAGIEIAKQLIFTQAHEV
                     ENIEKLIKFPIVMKVVGPIHKTEVDGVKLNIITTQQAVETFHYLMQIPDATAVLVQEL
                     VKGEELYCGAAKQGDFGHVVLCGLGGIFLELINDTASAMAPMSYSEVRLMVESLRGYP
                     LLKGYRNKKGLNEEKFIDIIQRIGALVHIAPEISELDLNPIKAEDKNMVAVDARICID
                     KINK"
BASE COUNT          775 a          438 c          328 g          857 t
ORIGIN      
        1 aaatacctgg tattggagaa gttgccagaa gttttggcca ggagcttaca ccttactcaa
       61 tgttgtccag aagtttagga ggattaaaag gaaatacatt gataatagct ttaccaggtt
      121 caacaaaagg tgcatcagaa agtatgcatg ctttatttcc ttatatacta cacttattta
      181 aagtaatgga ttattttagg catgaagggt aatgttattt atttatttta tcaatacata
      241 ttcttgcatc tactgcaacc atatttttat cttcggcttt aattggattt aaatccaact
      301 cacttatttc tggtgcaata tgtactaatg caccaattct ttgaataata tcaataaact
      361 tttcttcgtt taggcctttt ttatttctat atcctttaag taatggataa cctcttaatg
      421 attcaaccat taacctaact tcgctataac tcattggagc cattgcagat gcagtgtcat
      481 taattagttc taaaaaaatt ccacctaagc cacataaaac cacatgtcca aaatcgcctt
      541 gttttgctgc accacagtac aactcttctc ctttaaccaa ctcttgtaca agtactgcag
      601 tagcatcggg tatttgcatt aaataatgga aagtttctac agcttgttgt gtggtaataa
      661 tattcaattt tactccatct acttctgttt tatgaattgg ccctactact ttcattacaa
      721 taggaaactt aattaatttt tcaatatttt caacttcatg agcttgagta aaaattagtt
      781 gctttgcaat ttcaatacct gctgcttcta ttaattctac acatgtagca gcatctaaat
      841 aaccattttg agcagtatta ataatagacc taatagaaac aatatccata tcgggcaata
      901 ttgttttttc gaacttatat tttgtagaaa catatacatg agccaaggct ttgcctaaat
      961 ttacttcatc cgaaaaattt actcttccct tattcaaaaa ttgttttatt tctttttcgg
     1021 catttattaa ggatggtaaa acaggataaa taggtttttt gcaactattc attttttcgt
     1081 caagcaattc atacacatct tttacattaa ataaaccggg gcttccaaaa accacaacca
     1141 ttgcatctac tgcttcgtag ttatcgcaaa aatctattat ttctcctaat tgttttgcag
     1201 tgcctgttgc taaaaaatct attggattat ttactgaaga accaggattt aattttgcca
     1261 aaagttcttg tgttctatca ctttcaaaag gaggtacgtt taaaccatta gatgttaaag
     1321 catctgtaag cataactgca gagcctcctg catgggttat tatggcaata ttttcgcctt
     1381 gtagtttttt agattgaaaa atactgccaa cagcaatcaa ctcatctcta ctgctacaat
     1441 aaacaatacc tgcttttcta aataaagctc taataatatt atcagatgtt gccaatgcac
     1501 cagtatgaga agaggcagct cttccgccag cttctgaata accagatttt atagcagcaa
     1561 tcctgcatcc tttttctatt aatgaagtag catgttttaa aaatttgaaa ggctttttta
     1621 cctgctctaa atacaataat ttaactttag aacttttctt ttcatcaaaa gtattatcta
     1681 aatattctaa aatttcttct acacctgttt gtgctgcatt gccaatggaa taaatattag
     1741 aaaaccttaa ccctgttaac aaagcagact ccattataaa aactgctgtt gctccagagc
     1801 ttgaaattaa ctcgcaacca ttagggtcat attcaggaac tggtgttgta aatacacctt
     1861 tataatgttg gttaattaca ccaacacaat taggcccaat taatgttgca ttggatttat
     1921 taattatttc tacaatttct ttttcaatgg ctatgccctg ctctcctgct tctgaaaaac
     1981 ctgcagaaaa aataataaat gcttttgctc cttttttaag taactcttta attgtttgca
     2041 aacacaaact tgcaggtatt gcaataatgg ctaaatcaac tgtatttatt ttatttatat
     2101 tatcgtagct tataacacct tctatattta aaggctttgg attaatggca taaattggac
     2161 cattaaaatt acccttaagc aagttttgta aaaccatacc acctggttta gtggtattat
     2221 ctgatgcacc aataacagct atacttttgg ggtgaattaa ttgagaaaca atcatactct
     2281 atataaatgt tttaaataca aatataaatt tgaaaactac atacctaaat gacctgagtc
     2341 atggccttgt aacttagctc tatgtttcta agtaacaaaa catgcaagca atcaatat
//
LOCUS       DAOQHP010000343         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32380_length_2388_cov_3.280754, whole genome shotgun sequence.
ACCESSION   DAOQHP010000343 DAOQHP010000000
VERSION     DAOQHP010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32380_length_2388_cov_3.280754"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLY81_09520"
     CDS             <1..213
                     /locus_tag="PLY81_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNF30506.1"
                     /translation="QIEEFCNNLKHILMAVSWGGYESLIIPKYATIAKNEFDSNNLWH
                     KSLRLYVGLEEPDYIINDLEQAFSRL"
     gene            267..1721
                     /locus_tag="PLY81_09525"
     CDS             267..1721
                     /locus_tag="PLY81_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF30507.1"
                     /translation="MKYLLLVLATCIFYQSYAQTTSEKWNLQRMVDYAMKNNISVKQA
                     DIQARITALQHQSNKLAVYPNANFSSGLGMQFGRSVDPTTNQFTTTQLLFNNFNLSTG
                     ITLYNWGKLKNTISSSDFAVKASEAEIERAANDIALNVATYYLAVLSSKQQIEITLSQ
                     IASTNEQLENTRKRVDAGSLPELNAAELEARVAQDSINYYNAKSNFEQNILNLKGLLN
                     LDMAVPFEIEIPTLDKIPVLPLAELEPALVLQQALTELPQQKAANLRYKSAEYAVKAA
                     KAGMYPTITFGGGLGTNFASSTNKITGVTLGASQFTGQFVNVNGTDYYIQQPTVQFTQ
                     GKKGFGEMWDGYGVQLSNNFRQNVGFQINVPIFNNGSAKLNYQNSKLQLKQSEIAIEQ
                     SKQKLQQDIYMAHNNAIAALQRFNASEKTVETALRSFEYAKKRYEVGLITTLDLITNQ
                     NNYLQAKLTRLNNQFDYIFRMKLLEFYKGKGLKL"
     gene            1740..>2388
                     /locus_tag="PLY81_09530"
     CDS             1740..>2388
                     /locus_tag="PLY81_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNF30508.1"
                     /translation="MSKKMIWIIVGLVLAIIILIGLKKAGIIGKEEGLKVAIEKVSKR
                     TIIETVTASGKISPEVEVKVSPDISGEIVELSVEEGDTVHRGQIVAKIYADIYASQRD
                     QAAAGVDQSKAAEANANASLAALDATLQQAYANYIRQKKLYDEKVISKAEYEQAEQTY
                     KTAQANYNAAKESIKGSKASIKSAQANLNRADKDISRTVITAPMNGVVNVLNVKKG"
BASE COUNT          943 a          335 c          419 g          691 t
ORIGIN      
        1 caaattgaag agttttgtaa taatttaaaa cacattttaa tggcagtaag ctggggtggt
       61 tacgaaagtt taataatacc taaatatgct acaattgcaa aaaatgagtt tgatagtaac
      121 aatttatggc ataaaagcct taggctgtat gtaggtttag aagaacctga ctatataatt
      181 aatgatttag aacaagcatt tagccgttta taatttttga gttaaaattt aaaaagggtt
      241 aactactttc tattattttt gtttaaatga aatatttatt acttgtttta gccacttgta
      301 ttttttatca atcttatgca caaacaactt ccgaaaaatg gaatttgcaa agaatggttg
      361 attatgcaat gaaaaataat atatctgtaa aacaagcaga tattcaagcc agaattacag
      421 ccttgcagca tcagtcaaat aagttagctg tttaccctaa tgccaatttt agttcaggtt
      481 taggaatgca gtttggacgt tcagtagatc ctaccaccaa ccagttcact actacacaat
      541 tactttttaa taattttaat ttaagtaccg gtattacttt gtataactgg ggaaagttaa
      601 aaaataccat cagttcctca gattttgcag taaaagcctc tgaagcagaa attgaaagag
      661 cagccaatga tattgcacta aatgttgcca cttattattt agcagtttta agcagtaaac
      721 aacaaattga aattaccctt tcacaaatag catctaccaa tgaacaatta gaaaatacaa
      781 gaaaaagagt agatgccggc tcattgccag aattaaatgc tgcagaatta gaagcaagag
      841 tagcacaaga cagtattaat tattataacg caaaaagtaa ttttgaacaa aatattttaa
      901 acttaaaagg cttattgaat ttagatatgg cagtgccttt tgaaattgaa attccaacac
      961 tagataaaat acctgtattg ccattggctg aattagagcc tgcattagta ttacaacaag
     1021 cattaacaga attacctcag caaaaagccg ccaacttaag atacaaaagt gcagagtatg
     1081 ctgttaaggc tgctaaagca ggtatgtacc caactattac ttttggtggt ggcttaggta
     1141 caaattttgc aagcagtacc aataaaatta caggtgttac tttaggtgca agccaattta
     1201 caggtcagtt tgttaatgta aacggaacag attattatat ccaacaacct actgtacagt
     1261 ttacacaagg caaaaaaggt tttggtgaaa tgtgggatgg ttatggtgta caattaagca
     1321 ataattttcg ccaaaatgta ggcttccaaa ttaatgtacc catttttaat aatggcagtg
     1381 caaaactaaa ttatcaaaat agcaaattac aattaaagca atcagaaatt gccattgaac
     1441 aatctaaaca aaaattacaa caagatattt atatggcaca taacaatgcc atagctgcct
     1501 tacaacgttt caatgcaagc gaaaaaacag ttgaaacagc acttagaagt tttgaatatg
     1561 ctaagaaaag atatgaagta ggtttaatta caaccttaga tttaataacc aatcaaaata
     1621 attatttaca agcaaaactt acaaggctaa ataaccagtt tgattatatt tttagaatga
     1681 aattattaga attttataaa gggaaaggat tgaaactata aatacaaaaa tgaagtagta
     1741 tgagtaaaaa aatgatatgg ataattgtgg gtttagtact ggcaattata attttaatag
     1801 gattaaaaaa agctggtata ataggtaaag aagaaggctt aaaagtagct attgaaaaag
     1861 tttctaaaag aacaataatt gaaactgtaa cagcaagtgg caaaatttcg ccagaagtgg
     1921 aagtgaaagt gagcccagat ataagtggcg aaattgtaga attaagtgtg gaggaaggag
     1981 atactgtaca tcgtgggcaa atagttgcaa aaatttatgc agatatttat gcttctcaaa
     2041 gagatcaggc tgctgcaggt gtagatcaaa gtaaagcagc agaagctaat gccaatgcaa
     2101 gtttggctgc attagatgcc acattacaac aagcttatgc aaattatatt cgtcaaaaaa
     2161 aactatatga tgaaaaagta atttcaaaag cagagtatga acaagccgaa caaacttata
     2221 aaacagctca agccaattat aatgcagcta aagaaagtat taaaggcagt aaggcttcta
     2281 taaaaagtgc acaagccaat ttaaaccgtg cagataaaga tataagcaga actgttatta
     2341 cagctcctat gaatggagtg gtaaatgtat tgaatgttaa aaaaggag
//
LOCUS       DAOQHP010000344         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32436_length_2385_cov_4.478970, whole genome shotgun sequence.
ACCESSION   DAOQHP010000344 DAOQHP010000000
VERSION     DAOQHP010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32436_length_2385_cov_4.478970"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLY81_09535"
     CDS             <1..392
                     /locus_tag="PLY81_09535"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30509.1"
                     /translation="VQQTAAKNNTYNEYSITDEQPLNGTSYYRIEAVDKDGKRTYTKT
                     EKVQSKIENEKLKIYPNPAKDIVNVSFPKIKQVTVAVIIGRKLITNNYNILNNVQLGI
                     SSLPKGIYSIIVVDIGNNIQSKIITVE"
     gene            440..1336
                     /locus_tag="PLY81_09540"
     CDS             440..1336
                     /locus_tag="PLY81_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF30510.1"
                     /translation="MKKTYSKWILFVVLCLIWGSSFMLMKWGMFDKNNNPTLSAYDVA
                     ALRILFAGIVMLPFVPSSIKKISSKYLVYVLLSGWLGSFFPAFLFCIAESKIDGSLAG
                     SLNALTSLFVIITGFLFFNVKSTRKKIIGVIIGLLGCTLITVANYSKPFEYIAYSGFV
                     IFATFMYGFNINLIQKKLSALNALSVAAISLTGLIIPSATVLFFSGYFSLPLLQHTYI
                     ISTLSSVVLGILGTSLATILFYVLIKRSGGLFASLVTYGIPFVAIGWGFYNNEFITHV
                     HIIALLVILIGVFIVNKKQPKL"
     gene            1417..>2385
                     /locus_tag="PLY81_09545"
     CDS             1417..>2385
                     /locus_tag="PLY81_09545"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNF30511.1"
                     /translation="MQIWKEYQEQHKERFLNELLELLRIPSVSARSEHKEDMIKCAET
                     VKQRLLEAGADKVEIYPTAGHPVVYGEKIVDATKPTVLVYGHYDVQPPDPLELWHSGP
                     FEPVIKDGKIFARGACDDKGQFYMHVKALETMVNTNTLPLNVKFLIEGEEEIGSMHLG
                     EFLKQHKELLQSDVILISDTAMLSMENPSIDVGVRGLTYIEVEVTGPNRDLHSGVYGG
                     AVANPLTILAKMISSLHDENNHITIPGFYDDVVESSEAERKQLAEAPYDEEEYKKDLG
                     VKALWGEKGFTTNERTGIRPTLEVNGLWGGYTGEGAKTVLPSKAFAK"
BASE COUNT          838 a          316 c          429 g          802 t
ORIGIN      
        1 ctgtacaaca aacagcagca aagaataata cttataatga atacagcatt acagatgagc
       61 aacctttaaa tggaacgagt tattatcgta tagaggcagt agataaagat gggaaaagga
      121 cttatactaa aacagagaaa gtgcaatcga aaattgagaa tgaaaaattg aaaatttatc
      181 caaatcctgc gaaagatatt gtaaatgtta gttttcccaa aataaaacaa gtaactgttg
      241 cagtcataat aggaaggaaa ttgataacta ataactataa tattttgaat aacgtacaac
      301 tcggtatttc atcacttcct aaaggtatat actccataat agttgtagat attggcaata
      361 acatacaatc taaaataatt acggtagaat aaaattcaac ttatttataa attactcttc
      421 ctttgcttaa atttattttg tgaaaaaaac ttacagcaaa tggattttat ttgtagtgct
      481 atgtttaata tggggtagtt cttttatgtt aatgaaatgg ggtatgtttg ataaaaacaa
      541 caaccctact ctttctgctt atgatgtagc tgctttaaga attttgtttg caggaattgt
      601 aatgcttcca tttgttccaa gtagtatcaa aaaaatttca agtaaatatc ttgtatatgt
      661 tttattaagt ggatggttag gaagtttttt tccagcattt ttattttgca ttgctgaatc
      721 taaaattgat ggctctttgg caggaagttt aaatgcttta acctctttgt ttgtaattat
      781 aacaggcttc ttgtttttta atgtaaagag tactcgtaaa aaaataattg gtgtaataat
      841 aggattatta ggatgcactt taattacagt tgccaattac agcaaacctt ttgaatacat
      901 agcatacagt ggttttgtaa tatttgccac ctttatgtat ggctttaata taaacctaat
      961 tcaaaaaaaa ttatccgcat taaatgcttt atctgttgct gctattagcc tcacaggttt
     1021 gataattcct tcagcaactg ttttattttt ttcaggttat ttttcattgc cacttttaca
     1081 acatacttat ataattagta ccttgtcaag tgtagtttta ggcatattgg gtacttcgct
     1141 tgcaactatt ttattttatg tattaattaa aagatctggt ggcttatttg cttcactggt
     1201 aacttatgga attccttttg tagcaatagg ttggggtttt tataataatg agtttattac
     1261 ccatgtgcac attattgctt tattggttat acttataggc gtttttattg taaacaaaaa
     1321 acagcctaaa ttgtaatacc agttaccttg ttttatagtt tttttttaca ctaaaaaaac
     1381 attcctactt tcgttgttca acaaaaattt ttagcaatgc aaatttggaa agaatatcaa
     1441 gaacaacaca aagaaaggtt cttaaatgag ttgttggagt tattaagaat acctagtgta
     1501 agtgcaagaa gtgaacataa agaagatatg attaagtgtg ccgaaactgt aaagcaaaga
     1561 ttattagaag caggtgcaga taaagttgaa atttatccca cagcaggaca tcctgtagtg
     1621 tatggagaaa aaattgttga tgcaaccaaa cctacagttt tggtttatgg gcattacgat
     1681 gttcaaccac cagacccttt agaactttgg catagtgggc catttgaacc agttattaaa
     1741 gatggaaaaa tttttgctcg tggagcctgt gatgataaag gacaatttta tatgcatgtt
     1801 aaagcattag aaacaatggt taacaccaac actttacctt taaatgtaaa attcttaata
     1861 gaaggggaag aagaaattgg tagcatgcat ttaggcgaat ttttgaaaca acataaagag
     1921 cttttacaat cagatgttat tttaattagc gatactgcta tgttaagcat ggagaaccca
     1981 agcattgatg ttggcgtaag aggcttaact tatattgaag tagaagtaac aggaccaaat
     2041 agagatttac atagtggtgt gtatggaggt gcagttgcaa acccattaac tattttagct
     2101 aaaatgattt cttctttaca cgatgaaaat aatcatatta ctatacctgg tttttatgat
     2161 gatgtggtag aaagcagcga agctgaaaga aaacaattag cagaagctcc ttatgatgaa
     2221 gaagagtata aaaaagattt aggtgtaaaa gcattatggg gcgaaaaagg atttacaacc
     2281 aatgagagaa ctggcataag accaacatta gaagtaaatg gtttatgggg tggctataca
     2341 ggagaaggtg caaaaactgt tttaccaagt aaagcttttg ctaaa
//
LOCUS       DAOQHP010000345         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32532_length_2381_cov_2.589424, whole genome shotgun sequence.
ACCESSION   DAOQHP010000345 DAOQHP010000000
VERSION     DAOQHP010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32532_length_2381_cov_2.589424"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLY81_09550"
     CDS             <1..516
                     /locus_tag="PLY81_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30512.1"
                     /translation="IWNRKYNQVQGSSNVKMYRGSLVIVLIFLINSLFAQSYDWQKTL
                     ISSGISFVGGAANGLHETIYHHYPKFKKVHPNANDNYWNPQISWKNKYSDWDNGDTSE
                     KYFGSKTFLVWTTDAKHLLSLTSNMSLIGSTCVITIGEKRPWWHYAIDLAASGIARSA
                     GFHLTYNLIYK"
     gene            519..863
                     /locus_tag="PLY81_09555"
     CDS             519..863
                     /locus_tag="PLY81_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30513.1"
                     /translation="MNEIVKNLPKVEQPQQPQVDNNISKIIIISVIMGVVNALCSAEI
                     FCLPTEFAVKLNDVVYNFSPMALLNIIGFVAVIVFRKWFLNTTKLPTWLNKILELITN
                     INPFKLRKSENN"
     gene            1298..1573
                     /locus_tag="PLY81_09560"
     CDS             1298..1573
                     /locus_tag="PLY81_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30514.1"
                     /translation="MTSKAKIINLLREKGEEQLGHILQYTDYKPHLYKKMLKKKEVHD
                     RIIRKKESTKGNNKLSSGDRSTDLDTNELPKESSEKHIIQQNNKNKK"
     gene            1573..>2381
                     /locus_tag="PLY81_09565"
     CDS             1573..>2381
                     /locus_tag="PLY81_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30515.1"
                     /translation="MATYTKEQIENVWFALNLNRLIPNNEAYSGAGLHNPNRSINMYK
                     WLQDALTLIGVGPISLPLNTKGDILTYTTTLAKLGVGSNGQVLTADSSTATGLKWATP
                     SGGYTSPLTTKGDIFVRSASDTRLPVGTNGQFLSADSSEATGLKWVAAPGGGTNIYNS
                     NGTLTSNRFMDLAHYTLQIEDSNKPIGGATGNMQYLLSTEVVNFILNKHESSGSGYDH
                     IATMFLTQIPSTLAQVTSKCSTEDSNSGNIVWSEVTAQTTSSGNSAAFLSIT"
BASE COUNT          913 a          344 c          393 g          731 t
ORIGIN      
        1 atctggaaca ggaagtacaa ccaagttcag ggcagtagca acgttaagat gtacagaggt
       61 agcttggtaa tagtattaat atttctaatt aattcattat ttgctcaatc ttatgattgg
      121 caaaagactt taatatcttc aggtatatca tttgtaggag gggcagctaa tggcttacat
      181 gaaacaatat atcaccatta tcctaaattt aaaaaggtac accctaatgc aaacgataat
      241 tactggaatc ctcaaatatc gtggaaaaat aaatacagtg attgggataa tggagacaca
      301 tcagagaaat attttggtag taaaaccttt ttagtttgga ctactgacgc aaaacatctg
      361 ttatcattaa catctaatat gagtttaatc ggctctacgt gtgtgataac aataggagaa
      421 aaaagacctt ggtggcatta tgcaatagat ttagcagcat caggtatagc acgtagtgct
      481 ggatttcatt taacatacaa tttaatttac aaataacaat gaacgaaata gtaaaaaatc
      541 tacctaaggt agaacaacca caacaaccac aagtagataa taacatatct aaaataataa
      601 tcatatcagt tataatgggt gtagttaatg cattatgttc tgctgagata ttctgcctac
      661 cgacagaatt cgcagttaaa cttaatgatg tagtatataa cttttcacct atggcactgc
      721 ttaatattat aggatttgta gcagtaatag tatttagaaa gtggtttcta aataccacaa
      781 aattgcctac ctggttaaat aagatattag aattaataac taatataaac ccatttaaat
      841 taagaaaaag tgaaaacaac tagtttcgta ttacctatat taggtagtgt tgtattacct
      901 atgccagtac ttttaatatg gttagcaatt actattacac tagattttat aactggtata
      961 attaaaagta ctagcatagg ggataagaga gtttctaaga agtacagaga aactgtaggt
     1021 aaaatatttc aatactgtgg gacaatacta ataagtatta tacttagttc tgtagtaatt
     1081 acaaactctg tacctaatgt gttgtatgat aaaattattt cttattttac taatacaatt
     1141 gtagggttca tgatctttat agaaactact tctatttttg agaacttata tacaatagat
     1201 caaaaatcaa agttttctag atattttatt aaacctatgt taacattatt aacatttaga
     1261 atcaaactgt tttcagatac aattaacctt aaaaaagatg acaagtaagg cgaaaataat
     1321 taatcttcta agagaaaaag gagaagaaca attgggacat attcttcaat atactgacta
     1381 taaaccacat ttatataaaa agatgctaaa gaaaaaagag gtacatgata gaataattag
     1441 aaaaaaagaa agtactaaag gaaataataa gctatctagc ggtgatagat ctactgatct
     1501 agatacaaat gaactaccta aagaatcttc tgaaaaacat attatacaac aaaataataa
     1561 aaataaaaag taatggcaac atatacaaaa gaacaaatag aaaatgtatg gttcgcattg
     1621 aatctaaata ggctaatacc taataatgaa gcttactctg gagcaggttt gcacaatcct
     1681 aataggtcta taaatatgta caaatggcta caagatgctc ttactttgat aggagtagga
     1741 ccaatctcat tacctttaaa tactaaggga gacatattaa cttacactac tactttagct
     1801 aagttggggg taggatctaa cggacaagta ttaactgcag attcttctac agctactgga
     1861 ttaaagtggg ctactccttc tggaggatat acatctcctc ttactactaa aggagatatt
     1921 tttgttagaa gtgcgtccga tactagatta ccagtaggta ctaatggtca attcttatct
     1981 gcagattcaa gtgaagctac tggattaaaa tgggtagctg ctcctggtgg gggtactaat
     2041 atctataatt ctaatggtac attaactagc aatagattta tggatttagc acattataca
     2101 cttcaaatag aggatagtaa taaacctatt ggcggtgcta ctggaaatat gcagtactta
     2161 ttaagtacag aagtagttaa ttttatttta aacaaacatg aatcttctgg atcaggatat
     2221 gaccatatag caacaatgtt tttaacacaa ataccatcaa cattggctca agtaacgtca
     2281 aaatgttcca cagaagattc caatagtggc aatatagtat ggagtgaagt tacagcacaa
     2341 actacatcat caggaaatag tgcggcattt ttatcaatta c
//
LOCUS       DAOQHP010000346         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32603_length_2377_cov_3.700689, whole genome shotgun sequence.
ACCESSION   DAOQHP010000346 DAOQHP010000000
VERSION     DAOQHP010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32603_length_2377_cov_3.700689"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLY81_09570"
     CDS             <1..378
                     /locus_tag="PLY81_09570"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNF30516.1"
                     /translation="YNNNWYNRTKSAYQLLYKSSQPNATHLDTLNLAKIHPKLQGLSL
                     SSLQKFRNAFRQDRDYSVLWFVVVWGLNVVDATVFGHLKEFNVSDNLSMQIKPSINPI
                     NQAKGLSLVFNINKPIIRTAKVR"
     gene            417..1142
                     /gene="dapB"
                     /locus_tag="PLY81_09575"
     CDS             417..1142
                     /gene="dapB"
                     /locus_tag="PLY81_09575"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNF30517.1"
                     /translation="MKIALIGYGKMGKAIEEIAVAKGHEIVLKISTNNLSDFNESNLK
                     HADVAIEFTNPHSAVKNIEQCLNFGLPIVCGSTGWLNDWHGIENLCKQKNGTLLYASN
                     FSIGVNLFFELNTFLARLMSQQKNYDVMIEEIHHTQKKDAPSGTAITLAEQVLQYLTQ
                     KNKWVNYTAEQTNELQIISERKDPAPGTHKIKYTSTIDDIEIIHTAHNRKGFANGAVL
                     AAEYIFNKKGIFTMKQVLGLEIF"
     gene            1239..>2377
                     /locus_tag="PLY81_09580"
     CDS             1239..>2377
                     /locus_tag="PLY81_09580"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30518.1"
                     /translation="MKKVLLIIFLLATVYTINAQKNKPIIDSIEKALPTQSDSAKVKS
                     YSELTWQYRSINREKAIYYGNKGIALAKKINYQEGLAQVYNDLGIIYYDKELYDSSII
                     LYNAALQIRTKLNDDLGIAKLFNKIGIVYQRQGKFGEALYNQQNALSLFEKTNFLLGI
                     SYSLNNIGILQQNLGRYNEALDYHHQSIVIKQKLKDKNGLLQSYINIANIYKITNENT
                     KAENFYKKAIELSKELENNEYLANSLNNLGNLQFSLNKLEEGKQSILESYKIRTELKD
                     SKGRVSCLTNLANILMSLNKYDSAKQVLSTAEEIALKAVNTKPELILVYQTLSKLNEI
                     TGNATASLNYYKMYAAYKDSIYSNDLSNKFSELETKYKTAQKEKQ"
BASE COUNT          971 a          324 c          363 g          719 t
ORIGIN      
        1 tataacaata attggtataa tcgtactaaa agtgcttacc aacttttata caaatcatca
       61 caacccaatg ctacacattt agatactcta aatttagcaa aaattcaccc aaaactacaa
      121 gggctaagct taagctctct tcaaaaattc agaaatgctt ttaggcaaga tagagattac
      181 tcggtacttt ggtttgttgt tgtttggggc ttgaatgtag tagatgcaac tgttttcgga
      241 catttaaaag agtttaatgt tagcgataat ctttcaatgc aaattaaacc tagcattaat
      301 cctataaatc aggcaaaagg gttaagtttg gtttttaaca tcaataaacc tataattcgt
      361 acagccaaag tacgataaaa ttgtaggatt ttttttcatt ttttaaaact aagtgtatga
      421 aaattgcttt aataggatat ggcaaaatgg gaaaagcaat agaggaaatt gctgttgcaa
      481 aagggcatga aattgtatta aaaatttcta ccaataactt gtccgatttt aatgaaagca
      541 acttaaaaca tgccgatgtt gctattgagt ttactaaccc acatagtgct gtaaagaata
      601 tagaacaatg tttaaatttt ggtttgccaa ttgtttgtgg aagtacaggc tggctaaatg
      661 attggcatgg tattgaaaat ttgtgcaaac aaaagaatgg cactttatta tacgccagta
      721 actttagtat aggtgtaaat ttattttttg agttaaatac ttttttagca aggttaatga
      781 gccagcaaaa aaattatgat gtaatgatag aagaaataca ccatacacaa aaaaaagatg
      841 ctccaagtgg tacagcaata actttggcag aacaagtttt acaatactta actcaaaaaa
      901 ataaatgggt aaattatacc gcagaacaaa caaatgagtt acaaattatt tcagaaagaa
      961 aagaccctgc acccggcaca cataaaataa aatatacctc tactattgat gatatagaaa
     1021 ttattcatac agcccacaat cgcaaaggat ttgcaaacgg tgcagtacta gctgcagaat
     1081 atatttttaa caaaaaagga atttttacaa tgaaacaggt actagggtta gaaatttttt
     1141 aagaaatttg ttatgcaatt tgtgtctaat tttactctat aactaagaag ataaataaat
     1201 agaagaaact attatatatt taacacaaag gcatgcaaat gaaaaaagta ttactgatta
     1261 tttttttatt agctacagtt tacactatta atgctcaaaa aaataagcct attattgata
     1321 gcattgaaaa agctttgcct acacaaagtg atagtgcaaa agtaaaatca tatagtgaac
     1381 ttacttggca atatagaagt ataaacagag agaaagccat ttattacggc aacaaaggaa
     1441 ttgctttagc caaaaaaatt aattatcaag aggggttagc acaagtttat aacgacttgg
     1501 gcattattta ttatgataaa gaactgtacg actcatcaat tattttatac aacgcagcct
     1561 tgcaaataag aactaaatta aacgatgatt taggtatagc aaaattgttt aataaaattg
     1621 gcattgtata tcaacggcaa ggtaaatttg gagaagcact ttataaccaa caaaatgcac
     1681 taagcctttt tgaaaaaaca aattttttat taggaatttc ttattccctc aataatattg
     1741 gcattttaca acaaaattta ggaaggtaca atgaagcttt agactatcat catcaatcta
     1801 tagttattaa acaaaagctt aaagacaaaa atgggctact gcaatcttat ataaacattg
     1861 ccaatattta taaaattacc aacgaaaata caaaagcaga aaatttttat aaaaaagcaa
     1921 ttgagctttc taaagaatta gagaataatg agtatttagc aaatagttta aacaacttag
     1981 gcaacttaca attcagctta aataaattag aagaaggtaa acaaagtatt ttagaatctt
     2041 ataaaataag aactgaatta aaagactcta aaggcagggt gagttgttta actaatttag
     2101 ccaatatatt aatgagttta aataaatacg attcagcaaa acaagtatta tcaacggcag
     2161 aagaaattgc tcttaaagca gtaaatacta aaccagagtt gatattggtt tatcaaacac
     2221 tatctaaact taatgaaatt acaggcaatg caactgcatc cttaaattac tataaaatgt
     2281 atgcagcata taaagattct atttatagta atgatttgag taataaattt agtgagctgg
     2341 aaacaaaata caaaactgca caaaaagaaa aacaaat
//
LOCUS       DAOQHP010000347         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_32763_length_2371_cov_3.047927, whole genome shotgun sequence.
ACCESSION   DAOQHP010000347 DAOQHP010000000
VERSION     DAOQHP010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32763_length_2371_cov_3.047927"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /locus_tag="PLY81_09585"
     CDS             complement(<1..2296)
                     /locus_tag="PLY81_09585"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNF30519.1"
                     /translation="MSNKDIRREQALEYHAQGKPGKIEVIPTKEAKTQRDLSLAYSPG
                     VAEPCKEIAKDIDNVYKYTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGVLFKV
                     FADIDVFDIEINEKDPVKFVEIVKALEPTFGGINLEDIKAPECFYIEKELKKQLKIPV
                     MHDDQHGTAIISAAALLNALELQKKKIEKVKVVVNGAGAAAISCIKLYVALGVKKENV
                     KVFDSKGLITKQRTDLDEQKLEFAIDCEDMTLAEGIKNSDVFIGLSKGNVVSKEMILS
                     MAKHPIVFAMANPDPEISYEDATDARNDVIMATGRSDFPNQVNNVLGFPYIFRGALDV
                     RATTINEEMKLAAVRALADMAKTPVPDIVNLAYNLKNLSFGTDYIIPKPVDPRLISIV
                     APAVAKAAIESGVAKKTITDWKAYELELNKRLGLDNQLLRAIGNKARRDPKRLVFAEA
                     DNDKILKAASIIYDEGIAYPILLGTEKKIREIANEHNIDLTDIPIIDPRDEQYESKRE
                     LYGELFFAKRQRKGFNHYESIKIMKERNHFGCMMVETGDADAMISGLKRNYAEAIRPA
                     LQIIGTEEGVQKIAGMYLLSTQKGPIFLADTTINYNPTAEELADIALLVAKEVRSFNI
                     TPRIAMLSYSNFGSSNSHEARLVAEATSILKQKNPSLIVDGEMQGSLAFNKEVLKENY
                     PFSNLIDENVNVLIFPNLTAGNIAYNLLKELGGADAIGPILLGLKKPVHVLQLGSSIR
                     NIINVANIAVIDAQVKNKLDTDTEV"
BASE COUNT          657 a          447 c          328 g          939 t
ORIGIN      
        1 gaacttctgt gtctgtatct aatttgtttt ttacttgtgc atctattact gcaatattag
       61 ctacattaat tatatttctt atactgctac ctaattgtaa aacatgaaca ggttttttta
      121 accccaataa aataggacca attgcatctg ctccacctaa ttcttttaat aaattataag
      181 cgatattacc tgctgttagg ttagggaaaa ttaaaacatt tacattttcg tcaatcaaat
      241 tactgaatgg ataattttct tttagcactt ctttgttgaa tgcaagactg ccttgcattt
      301 cgccatctac aattaaggat ggattttttt gtttaagtat gctggttgct tctgcaacta
      361 atcttgcttc gtggctattg ctactaccaa aattactata gcttaacata gcaatacgtg
      421 gtgtaatatt aaaacttctt acttctttag caactaataa agcaatatct gccagttctt
      481 ctgcagttgg gttataatta atagtggtat ctgccaaaaa tattgggcct ttttgtgtac
      541 ttagtaaata catgcctgca attttttgta caccttcttc tgtaccaata atttgtaatg
      601 caggtctgat ggcttcggca taatttcttt ttaaaccact tatcattgca tcggcatctc
      661 ctgtttctac catcatacaa ccaaagtggt ttctttcttt cattatctta atactttcgt
      721 agtggttaaa gcctttgcgt tgacgttttg caaaaaacaa ttcgccataa agttctcttt
      781 tgctttcgta ttgttcatct ctagggtcaa taattggaat atcggttaaa tcaatattat
      841 gttcattggc tatttctctt attttctttt ctgttcctaa taaaataggg taggcaattc
      901 cttcatcata aataatactt gctgctttta aaattttatc gttatcggct tcggcaaata
      961 ccaagcgttt tggatctctt cttgctttgt taccaattgc tcttaataat tggttatcta
     1021 ggcctaatct tttatttagt tcaagttcat aagctttcca atcggtaata gtttttttag
     1081 ctacaccact ttctatcgca gcttttgcta ctgctggtgc tacaatagaa atcaatcttg
     1141 ggtcaactgg ttttggaata atataatcag ttccaaaaga taaatttttt aaattatagg
     1201 caaggtttac aatatctgga acaggtgttt ttgccatatc tgccaatgct cttacggctg
     1261 ctaatttcat ttcttcgtta atggttgttg ccctaacatc taaagctcct ctaaaaatgt
     1321 atggaaaccc taaaacatta ttcacctgat taggaaaatc gcttctaccg gttgccataa
     1381 taacatcatt tcttgcatct gttgcatctt cataactaat ttcaggatca ggattagcca
     1441 ttgcaaaaac aattggatgt tttgccatgc ttaatatcat ttctttgctt actacattgc
     1501 ctttacttaa tccaataaaa acatcgctat tttttattcc ttctgccaat gtcatatcct
     1561 cacaatctat tgcaaattct aatttttgtt catctaaatc tgttcgttgt ttagttatta
     1621 atcctttgct atcaaatact tttacatttt cttttttaac tccaagtgca acatagagtt
     1681 ttatacaaga aatggctgcc gctccagcac cattaaccac tacttttact ttttctattt
     1741 tctttttttg cagttctaat gcatttagta aagcagctgc acttattata gcagtgccat
     1801 gttggtcatc atgcattaca ggaattttca attgtttttt taattccttt tcaatataaa
     1861 aacattcagg tgctttaata tcctctaagt ttatacctcc aaatgtgggt tctaatgctt
     1921 ttacaatttc aacaaacttt acagggtctt tttcattaat ttcaatatca aaaacatcaa
     1981 tatcggcaaa tactttaaat aaaacacctt ttccttccat aactggtttg ccagcttcag
     2041 ggcctatatc tcctaagcct aaaactgctg tgccattgct tattacagca actaaattac
     2101 cttttgctgt gtatttgtaa acattatcaa tatcttttgc aatttcttta catggctctg
     2161 ctacacctgg gctatatgcc aatgaaaggt ctcgttgtgt ctttgcttcc tttgtaggaa
     2221 ttacttcaat cttgcctggt tttccttgtg catggtattc taatgcttgt tctcttctta
     2281 tatctttatt gctcataggt tttatacttt attgattgga atagaaataa aattgttttt
     2341 aagcttaaag tgaaatagtg caaaatacaa t
//
LOCUS       DAOQHP010000348         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_33155_length_2355_cov_3.662174, whole genome shotgun sequence.
ACCESSION   DAOQHP010000348 DAOQHP010000000
VERSION     DAOQHP010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33155_length_2355_cov_3.662174"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1302
                     /locus_tag="PLY81_09590"
     CDS             88..1302
                     /locus_tag="PLY81_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HNF30520.1"
                     /translation="MKTYCKTLLLFMVLFANLIVVAQKKTAKRLGCNTADPINSFAFD
                     TTQPTRGMADNNFLWDNGKTINVKILSGSNALKTKVQKWAKEWMKYANIKFNFIDKGE
                     AQIRVFLGDKKGNYGHVTMGLGIRCLQRDANDFTMHFDTTDFVYEANDKAFRGTVLHE
                     FGHALGLIHEHMSPVSGIQWDSSAVYARKAMMGWNKDMTNAQLFRKYNISYTNGTQYD
                     PKSIMHYPISKWETKNRYWVDWNYDLSEGDKALIAALYPKGKRENKVPRITISNVGET
                     IIEYNHNRRGICIYPSFDITCADKEAEIYYVSLLIDENSYFLPTQSKDYQINKYAGLA
                     KAIKLPLNKVEKINKGTKNFELFIPDSELPSNIIGKKINVYFRVFLNNNGEFKDVFAN
                     ISSKGITINNLY"
     gene            complement(1366..1992)
                     /locus_tag="PLY81_09595"
     CDS             complement(1366..1992)
                     /locus_tag="PLY81_09595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNF30521.1"
                     /translation="MKKWLEEVVLETQHIKLIPINENHKADLLNASTDGNLSELWVTS
                     VPTINNIDKYLAKAIEDYKQDKGLAFVVINKKTNSVIGTTRYTNATPEHRRLEIGYTW
                     YSKSFQKTHINSECKLLLLTHAFEILKAIAVEFRTNWYNFPSRNAIARLGAKQDGVLR
                     NHQIMPDGSLRDTVVFSILESEWNTCKNALMYKIEQIHKKYPAIKDNL"
BASE COUNT          835 a          325 c          346 g          849 t
ORIGIN      
        1 ccaatccgtt aaattataaa atacctttca atgggtacaa atttttcata aaacatgatt
       61 aactttaggt ttattaaatt acactttatg aaaacatatt gtaaaacact tttattattt
      121 atggtgcttt ttgcaaacct tattgttgtt gctcaaaaaa aaacagccaa acgtttaggt
      181 tgcaatactg ccgatccaat taatagtttt gcttttgata ctacccaacc tacaagaggt
      241 atggcagata ataatttttt gtgggataat ggtaaaacaa taaatgtaaa aatattaagt
      301 ggtagcaatg ctttaaaaac taaagtgcaa aaatgggcta aggagtggat gaaatatgca
      361 aatattaaat ttaactttat tgacaaagga gaagctcaaa taagagtttt tttaggcgac
      421 aaaaaaggga attatgggca tgtaacaatg ggcttaggaa taagatgctt acaaagagat
      481 gcgaatgatt ttaccatgca ttttgatact actgattttg tttacgaagc taacgataaa
      541 gcatttagag gtactgtttt acacgagttt ggccatgcat taggtttaat acacgaacac
      601 atgagccctg taagtggaat acaatgggac agttcggctg tttatgcaag aaaagcaatg
      661 atgggttgga ataaagacat gacgaatgct caacttttta gaaaatataa tatatcctat
      721 accaatggta cacagtacga cccaaaatca attatgcatt acccaatttc taaatgggaa
      781 accaaaaata gatactgggt tgattggaat tatgacttat ctgaaggaga taaagcttta
      841 attgcagcat tataccctaa aggtaaaaga gaaaataaag tacctcgtat aaccatttca
      901 aacgttggcg aaacaataat tgaatataac cataacagaa gaggtatttg catttaccct
      961 agttttgata ttacctgtgc cgataaagaa gctgaaattt attatgtaag tttattaatt
     1021 gatgaaaatt catacttctt acctactcaa agtaaagact atcaaataaa taaatatgca
     1081 ggtttagcta aggcaataaa acttcctcta aacaaagtag aaaaaataaa taaaggcact
     1141 aaaaattttg aattatttat ccccgatagt gagttgccaa gcaatataat agggaaaaaa
     1201 attaatgttt attttagagt attcttgaac aataacggag agtttaaaga tgtttttgcc
     1261 aacatttcct ctaaaggtat tactataaat aatttatatt aagtataact ttttatatct
     1321 cagcaaaaaa aattaagatt taaaacgagt ttatttatcc agctattata aattatcttt
     1381 aatagctgga tattttttat gaatctgttc tattttatac attaatgcat ttttgcaagt
     1441 attccattca ctttccaata tagaaaaaac aacagtatct cttagagagc catcaggcat
     1501 aatttgatga tttcttaata ctccatcttg tttagcacca agtcttgcaa tagcatttct
     1561 tgatggaaaa ttataccaat tagttctaaa ctctactgct attgctttaa gtatttcaaa
     1621 agcatgtgtc aataatagta atttacattc tgaatttata tgtgtttttt gaaatgattt
     1681 tgaataccaa gtatatccaa tttctaatct tctatgttct ggtgttgcgt tagtatatct
     1741 agtagtgcca attacactgt tcgttttttt atttattact acaaaagcca atcccttgtc
     1801 ttgtttataa tcttcaattg cctttgctaa atatttatca atattattaa ttgttggtac
     1861 tgatgttacc cataattcag ataaatttcc atcggtagat gcatttaata aatctgcttt
     1921 atgattttca ttaattggaa taagctttat gtgttgggtt tctaaaacaa cttcttcgag
     1981 ccattttttc attttggttt tgtttcaatt tttttgtttc tataatcttg ttattataat
     2041 aacagaatcg tcgttaaatg tacccatttc caccaatatt ttatactact attcattaat
     2101 agaaatagta aaattattta gtaagtaaca attggttagc aattatttta ttatgttata
     2161 ttttttaggt tttaaatgag aatattttat ttgaaacata tcttaaaaaa aattgagcca
     2221 cattaagtgg ctcaattttt ttataacaag aatatgtatg caattaatat tttgtaaact
     2281 taaatactgc agtttcgcag ccattgttgc atattgcttt tattaaatat gctccctggc
     2341 ttagtctgct aacat
//
LOCUS       DAOQHP010000349         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_33332_length_2348_cov_3.825992, whole genome shotgun sequence.
ACCESSION   DAOQHP010000349 DAOQHP010000000
VERSION     DAOQHP010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33332_length_2348_cov_3.825992"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLY81_09600"
     CDS             <1..389
                     /locus_tag="PLY81_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30522.1"
                     /translation="DTSLSFFDKASFEQTNIMMGGITTFSDEVKPAPFTTFVKKVFNK
                     SFFKISPNPTNNNGVTISIKQPGNYVVQIFNNNSILVYTTEIEVSYKDEKVQIKFNGS
                     IAKGTYYIRVIDKKNNNQYTDKLLVN"
     gene            544..831
                     /locus_tag="PLY81_09605"
     CDS             544..831
                     /locus_tag="PLY81_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30523.1"
                     /translation="MNKHIFKLFKKKKVSVFPNPATKGKVVNLMINKGNYAVQLEDKN
                     SQAVYEQEIMVANKNEVKLFNIPSNINNGTYYIKVVNKNNQQVLRSCLKVV"
     gene            complement(836..1738)
                     /gene="xerD"
                     /locus_tag="PLY81_09610"
     CDS             complement(836..1738)
                     /gene="xerD"
                     /locus_tag="PLY81_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNF30524.1"
                     /translation="MWQPYKKGYKDWLQLEKSLSDNSVEAYLRDIEKLTEYLIVQNSP
                     KKINEISLKDLQLFIQWIGELGMTPTSQARIVSGIRSFFKYCLTEQLITSDPSILLET
                     PKVKRALPDVLSFEEIENIINQIDLSKPDGGRNKAIVETMYSCGLRVSELINLKISCL
                     YLDVGFIRVIGKGDKERLVPIGSDAIKYITIYKNNIRTNIVPKQGFEDILFLNKFGKS
                     ISRVMIFYIIKELVLKANIDKNISPHTFRHSFATHLVEGGANLRAVQEMLGHESITTT
                     EIYTHLDREFLRTTLTQFHPAFKK"
     gene            1855..>2348
                     /locus_tag="PLY81_09615"
     CDS             1855..>2348
                     /locus_tag="PLY81_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF30525.1"
                     /translation="MQPVNLRSFKQKVFHHKKAFKKFLSKVEKNPPKNINSIANKTNE
                     AVWQEIDCLSCANCCKVMSPTFTTNDIKRISTHLKLTTQEFKDKYLFYDKKDGDWMNK
                     KQPCQFLNLTTNKCNIYEIRPEDCAGFPHLKKKKITDYIHVHQQNIEYCPATYKMVEK
                     WMIA"
BASE COUNT          889 a          339 c          300 g          820 t
ORIGIN      
        1 aagacacttc gttatcattt tttgataaag caagttttga acaaaccaat attatgatgg
       61 gtggaataac aaccttttct gatgaagtaa aacctgcacc atttacaact ttcgtaaaaa
      121 aagtatttaa taaatctttt ttcaaaattt caccaaaccc tactaataat aatggcgtaa
      181 ctatttctat taagcaacca ggcaactatg tagtacaaat ttttaataac aattcaatac
      241 tagtttacac aacagaaatt gaagtaagct acaaggatga aaaagtgcaa attaaattta
      301 atggcagtat tgctaaagga acatattata taagagttat tgacaaaaaa aataataatc
      361 aatataccga taaactttta gtgaattaaa aatattaatg gttacaagta ttattatcta
      421 tatccattaa tgaatggtga gcaacaaaat tttcttatat tatgtaatta ttaaattctc
      481 ttcatctgta tttataattc aatcatcagt acaaaatttg cataattaaa atatattttt
      541 tttatgaaca agcatatttt taaactattt aaaaaaaaga aagtttctgt ttttcctaat
      601 cctgcaacaa aaggcaaggt tgtaaattta atgattaata aaggcaatta tgctgtacaa
      661 ttagaagata agaatagcca ggctgtatat gagcaagaga ttatggttgc aaacaaaaac
      721 gaggtgaaac tatttaatat tcccagtaat attaacaatg gcacttatta cattaaagta
      781 gtcaataaaa ataatcagca agttttaaga agttgtttga aagtagttta ataatttatt
      841 ttttaaaggc tggatgaaac tgtgttaatg tagttctcaa aaattctctg tctaaatgtg
      901 tgtaaatttc agtggttgta atactttcat gacctagcat ttcttgcact gctcttaaat
      961 ttgctcctcc ctctaccaaa tgtgttgcaa aagaatgacg aaaagtatga ggtgaaatat
     1021 ttttatcaat attagctttt aatactaact ctttaatgat ataaaaaatc attacacgag
     1081 aaatagattt accaaacttg tttaaaaaca aaatatcctc aaacccttgc tttggcacta
     1141 tattagttct tatattgttt ttataaattg taatatattt tatagcatcg ctaccaattg
     1201 gcaccagtct ttccttatcg ccttttccta ttactctaat aaagccaaca tctaaataca
     1261 aacaacttat tttaaggttt attaattcgc tcactcttaa accgcagcta tacatagttt
     1321 ctacaatggc tttatttcta ccaccatcag gtttacttaa atcaatttga ttgataatat
     1381 tttcaatctc ctcaaagctt aacacatctg gcaaagctct ttttactttt ggtgtttcta
     1441 ataaaataga tggatcactt gtaataagtt gttcggttag gcaatactta aaaaaacttc
     1501 ttatgccaga aactatacga gcttgcgaag ttggtgtcat accaagctct cctatccact
     1561 gaataaaaag ttgcaaatct tttaaactaa tttcatttat ttttttggga ctgttttgta
     1621 caattaaata ttccgtaagc ttctctatat ctcttaaata agcttctaca gaattatcgc
     1681 ttaaagattt ttctaactgt aaccaatctt tatatccttt tttgtatggc tgccacatta
     1741 ttaaagaagt aagttataaa agtaggagat gttttttatt tgaaagtaca attatgtaac
     1801 tatattcgtt tttaaaacaa gtacgaaaat ttattttcaa ttattaaatt tacaatgcaa
     1861 ccagttaatt tacgttcgtt taaacaaaaa gtttttcatc ataaaaaagc ttttaaaaaa
     1921 tttttaagta aagtagaaaa aaatcctcct aaaaatatta atagtattgc aaacaaaact
     1981 aatgaagcag tttggcaaga aatagattgt ttaagttgtg ctaattgctg taaagtaatg
     2041 agccctacat ttactacaaa tgatattaaa agaatttcta cccatttaaa attaaccact
     2101 caagaattta aagacaagta tttgttttat gataaaaaag atggcgattg gatgaataaa
     2161 aaacaacctt gccagttttt aaatttaaca acaaataaat gcaatattta tgaaattagg
     2221 ccagaagatt gtgccggatt tccgcattta aaaaagaaaa aaataaccga ttatattcac
     2281 gttcatcaac aaaatattga gtactgccct gcaacctata aaatggtaga aaaatggatg
     2341 attgctat
//
LOCUS       DAOQHP010000350         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_33386_length_2346_cov_2.755129, whole genome shotgun sequence.
ACCESSION   DAOQHP010000350 DAOQHP010000000
VERSION     DAOQHP010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33386_length_2346_cov_2.755129"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLY81_09620"
     CDS             complement(<1..263)
                     /locus_tag="PLY81_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNF30526.1"
                     /translation="MLVKTFGSAVYGVEAISITIEVNVSGGDLKYYMVGLPDNAVKES
                     LQRIESAIKTNQLSFPRTKIVINLAPADIKKTGSAFDLPIALG"
     gene            371..1252
                     /locus_tag="PLY81_09625"
     CDS             371..1252
                     /locus_tag="PLY81_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNF30527.1"
                     /translation="MILLDGKLASASVKENLKKEVDKIIEKNLRPPHLAAILIGNNGA
                     SETYVASKIKSCKEVGFTSTLIRYEESVLEKVLLDKILELNNDPTIDGILVQLPLPKH
                     IVETKVIETINPKKDVDGFHPINAGKLVQGLETLIPATPYGVMLMLEHFNVDTKGKHA
                     VIIGRSNIVGRPMSILLSSNLLHGNCTVTLCHSHTPDLIEICKQADILVAALGKPESI
                     TAKMVKPGAVIIDVGITRIDDNTKKNGYRLKGDVKFDEVAAIASAITPVPGGVGLMTI
                     AGLLKNTLKAYQLNTKN"
     gene            1282..1923
                     /locus_tag="PLY81_09630"
     CDS             1282..1923
                     /locus_tag="PLY81_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNF30528.1"
                     /translation="MRKTSMLYPVMESFYTLQGEGFYTGAAAYFIRLAGCDVGCVWCD
                     VKESWNANEHPKYSVNDIIEKVLIEVNYHSISNKTNLPIIVITGGEPLMHNLNELTKA
                     LQQNGFKTNIETSGSSALSGSWDWICLSPKKFKPPIKENIVLANELKIIVYNKQDFKW
                     AESYAKQVNSHCKLYLQPEWSKRELITPLIVEYIKLNPQWQLSLQTHKYINVP"
BASE COUNT          861 a          318 c          382 g          785 t
ORIGIN      
        1 attcctaagg caattggtaa atcaaaagca ctgcctgttt tttttatatc agcaggagct
       61 aagtttataa caattttcgt tctaggaaaa ctaagctgat tagttttaat ggcactttca
      121 attctttgca aactttcttt cactgcatta tctggtaaac ctaccatgta gtattttaaa
      181 tcaccaccac taacattcac ttctatagta atagaaattg cttcaacacc atatacggca
      241 ctgccaaatg tttttacaag catggtaggt ttttaaaaaa ataatagttt attacttgta
      301 atattaagaa taaaatactt ataaaccatt tttttactgc tgcatagttt gtagatatta
      361 actttgcagt atgatattgt tagatggaaa attggcttcg gctagtgtta aagaaaatct
      421 taaaaaagaa gttgataaga taattgaaaa gaatcttaga cctcctcatt tagcagcaat
      481 tttaataggc aataatggtg caagcgaaac ttatgtagcc agtaaaataa aaagttgtaa
      541 agaggttggt ttcacttcta ctttaattag atatgaagaa tctgtattgg aaaaggtatt
      601 actagataaa attctagaat taaacaatga tcctactata gatggcattt tagttcagtt
      661 acccttacct aaacatattg ttgaaactaa agttatagaa actataaacc ctaaaaaaga
      721 tgtagatgga tttcacccaa taaatgcagg aaaacttgta caagggttag aaactttaat
      781 acctgctaca ccttatggag taatgctaat gctagaacat tttaatgtag atactaaggg
      841 taaacatgct gttattattg gtagaagtaa tattgtgggc aggccaatga gcattttact
      901 tagtagtaat ttattgcatg gaaattgcac agttacctta tgtcatagcc atactccaga
      961 tttaatagaa atttgtaagc aagccgatat tttggttgca gcattaggta aaccagaatc
     1021 tattactgcc aaaatggtaa aaccaggtgc tgtaattatt gatgttggaa ttactagaat
     1081 tgatgataac accaaaaaga atggctatcg cctaaaaggt gatgtaaaat ttgatgaagt
     1141 agctgctatt gcaagtgcta ttactcctgt accaggtggt gttggtttaa tgaccattgc
     1201 agggttatta aaaaatactt taaaagccta tcaactaaat actaaaaatt aagtaaaaga
     1261 aaaatcgttt aatcattttt aatgcgtaaa acttctatgt tatatcctgt aatggagagc
     1321 ttttatacct tacaaggcga agggttttat acaggggctg cagcatattt cattaggctt
     1381 gcaggttgcg atgttggttg tgtttggtgc gatgtaaaag aaagttggaa cgccaatgag
     1441 cacccaaaat atagcgttaa tgatattatt gaaaaagttt taattgaagt aaattatcat
     1501 tcaattagta acaaaacaaa tttaccaata attgttatta caggtggcga gcctttaatg
     1561 cataatttga atgagttaac aaaagcacta caacaaaatg ggtttaaaac aaatattgaa
     1621 actagtggat catctgcatt aagtggtagt tgggactgga tttgcctttc gccaaaaaaa
     1681 tttaaacctc caattaaaga aaacattgta ctagcaaatg agttaaaaat tatagtgtac
     1741 aacaaacaag attttaagtg ggcagaaagt tatgccaaac aagtaaatag ccattgtaaa
     1801 ctttatttac aaccagagtg gagtaaaaga gaattgataa ccccattaat tgttgagtat
     1861 attaaattaa accctcaatg gcaattaagc ttacaaacac ataagtatat aaatgttcct
     1921 taaacgaact tgttagttta atttaatgta actcttttca atataagtat ggtttgttgt
     1981 aacattaact ttcaaattgc tttaaagcaa caataaaaaa tcatatttat aattataact
     2041 ttagtttttt tattagcagt ttctatgtta aaaataagtt ttcattagtg gtttgcaaat
     2101 acatttttag ttttgaaaat atttaccttt tttgctattt aacaaataaa caaatctcct
     2161 aattatccat atttaaaaaa ttggtattac ctttcgcaaa caatgaaaaa tattgtttta
     2221 ctttttgcat gtatattttg tattaacgta tatgcacaaa cttataatcc tgaaaaaata
     2281 aataaaaaag ctgtaaaaat atatatgcag gctgtggagt atttggcaga tggtatgttg
     2341 ccagaa
//
LOCUS       DAOQHP010000351         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_33589_length_2337_cov_3.550394, whole genome shotgun sequence.
ACCESSION   DAOQHP010000351 DAOQHP010000000
VERSION     DAOQHP010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33589_length_2337_cov_3.550394"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLY81_09635"
     CDS             complement(<1..394)
                     /locus_tag="PLY81_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF30529.1"
                     /translation="MNISELSLRRPVLATVMNILIVLFGAVGYSFLSVREYPAIDPPM
                     INVSTSYAGANSDIIESQITEPIEKAVNGIPGIRSISSSSQVGRSSISIEFNVNENLE
                     AAANDVRDKVGQAIRNLPEDIDAPPVVTK"
     gene            complement(435..1490)
                     /locus_tag="PLY81_09640"
     CDS             complement(435..1490)
                     /locus_tag="PLY81_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF30530.1"
                     /translation="MRINLLIIIVISAFASCKENKQAEQNKGKAAQNTIVDVLLAGTK
                     SIQNVIEVNGTVIANETVTLHPEISGRLVYLNVPDGKQVAAGTLLAKINDAELQAQFN
                     KSKVQLELAQKTEERLRKLLQINGINQADYDIALNNVNNIKADIELVKAQLDKTLIKA
                     PFSGVLGLRQTSPGAYVTPQTTIATLQETNKIKIDFSVPEQFASLIKKGNTILFTTTE
                     NKVQQKATVIATEPEINTDTRNLKVRAIPNNSNINPGAFVKVFLKEGAVSNNIVIPTS
                     SIIPEASAKKVIVVKNGKGKFVEIVTGNRTATGVEVLSGLTVGDSVVVTGVLFVRPNA
                     DVKVRSVKTLDQLFNEN"
     gene            complement(1690..>2337)
                     /locus_tag="PLY81_09645"
     CDS             complement(1690..>2337)
                     /locus_tag="PLY81_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF30531.1"
                     /translation="STKALQKMQEKLLAENKHPSLQIETLSDYNTVMNGVKHAVKTLD
                     AKLIIMGITGSSAIEQSLIGSNTIDVAKEANVPVIIIPPNAVFRSIQNTFLACSLQGL
                     NENLPHTNIVDFLQKSKSNFGIVHIEKDLNKKYNDQDFLNTTVDSLFKMFNTKHQVVY
                     NPDFVEGINHFIDNNKVDLLIVIPKKHGFFDRLFNKSHTKMLAFHSHVPLMVIHD"
BASE COUNT          710 a          368 c          353 g          906 t
ORIGIN      
        1 tttttgtaac tactggtggt gcatcaatat cttctggtaa gtttctaata gcttgcccca
       61 ctttatctct tacatcattt gctgcagctt ctaagttttc gtttacatta aactcaattg
      121 aaatgctact tctaccaacc tggcttgatg aactgataga tcggattcca ggaattccat
      181 taactgcttt ttctattggt tcggtaattt ggctttcaat aatatcgctg ttagcaccag
      241 cataagaggt actaacgtta atcattggtg ggtcaatagc tggatattct cttacagata
      301 aaaatgaata gcctacggca ccaaataata caatcagaat attcattact gttgctaata
      361 ctgggcgtct taatgatagt tcggatatat tcataattct tagaaaaatc taaaaaaata
      421 tttttaataa agtattaatt ttcgttgaaa agttggtcta aagtttttac acttctcact
      481 tttacatctg catttggcct aacaaataat acaccagtta caacaaccga atctccaact
      541 gttaaaccac ttaaaacttc tacaccggtt gctgttctat ttcctgtaac tatttcaaca
      601 aattttcctt ttccattttt tactacaatt acttttttag cagaagcttc gggtataatg
      661 gaacttgtag gaataacaat attgtttgaa acggcacctt cttttaaaaa tacttttaca
      721 aatgcaccag gatttatatt tgaattattt ggaattgctc tcacttttaa atttcttgta
      781 tctgtattta tctctggctc tgtagctatt acagtggctt tttgttgtac tttattttcc
      841 gtagtggtaa ataaaattgt gttgcctttt tttattaagc ttgcaaattg ttctggtaca
      901 gaaaaatcaa ttttaatttt gttggtttct tgtaaagttg caatagttgt ttgaggggta
      961 acataggcac cagggcttgt ttgccttaaa cctaaaacgc cgctaaaagg tgctttaatt
     1021 aaagttttat ctaactgagc ttttactagt tcaatatctg ctttaatgtt atttacattg
     1081 tttagggcta tatcataatc ggcttggtta atgccattta tttgtaatag ttttcttaaa
     1141 cgctcttctg ttttttgagc aagttctaac tgcactttac ttttattaaa ttgagcttgt
     1201 aattcagcat cgttaatttt agctaataat gtaccagcag caacttgttt tccatctggc
     1261 acatttaagt aaaccaatct tccactaatt tccggatgta gtgttactgt ttcgtttgct
     1321 attacggtac cattaacctc tattacattt tgaatagatt tagtacctgc taataacaca
     1381 tctactattg tgttttgtgc tgcttttcct ttattctgtt ctgcttgctt attttctttg
     1441 caagatgcaa atgccgatat tacgatgatg attagaagat ttatacgcat aggtcaaagt
     1501 tggaatggct aagataacca aataaaattt ttatttaacg aggcttttat aagtggcgaa
     1561 aatatatttt aaagttgttt tacttccaat caatagagat gaacggtgaa ttttacctta
     1621 agttatacat atttttcctt tattcataaa gttaatggta aatatgaagt acaaaaagtg
     1681 gtgttatgtt taatcatgaa taaccattaa aggtacatga ctatgaaaag ccaacatttt
     1741 agtatggctt ttattaaaca acctgtcgaa aaagccatgt tttttaggaa tgacaattaa
     1801 taaatctact ttattattat caatgaaatg attgatacct tctacaaaat ctggattata
     1861 cacaacttga tgcttagtat taaacatttt aaataaggaa tcaacagtgg tatttaaaaa
     1921 atcttgatca ttatacttct tatttaaatc cttttctata tgaacaattc caaagttaga
     1981 tttagatttt tgcagaaagt caactatatt agtatgtggt aagttttcgt ttaaaccttg
     2041 taagctacaa gctaaaaaag tattttgaat agatctaaaa acagcatttg ggggtataat
     2101 aattacaggt acattggctt cttttgcaac atctattgta ttactgccaa ttaatgattg
     2161 ttctattgca gagctaccag taatgcccat tataatgagt tttgcatcta atgtttttac
     2221 agcatgtttc acaccattca taactgtatt gtaatcacta agtgtttcaa tttgtaagga
     2281 gggatgttta ttttctgcaa gtaatttttc ttgcattttt tgtaaagcct tagtgct
//
LOCUS       DAOQHP010000352         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_33716_length_2332_cov_4.049188, whole genome shotgun sequence.
ACCESSION   DAOQHP010000352 DAOQHP010000000
VERSION     DAOQHP010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33716_length_2332_cov_4.049188"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLY81_09650"
     CDS             complement(<1..1302)
                     /locus_tag="PLY81_09650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF30532.1"
                     /translation="MPGYELWGEEEKKHVNDVLETGILMRYGFDTPRKGIWKSIELEK
                     AITETFGCKYAQLTSSGTAALTTALAALGIGAGDEVIMPSFTFVASFEAVLSVGAVPV
                     LVDVDKTLTLNPKAVKNAITANTKCIMPVHMCGSMADLDALQAICKEHNLFLLEDACQ
                     SIGATYKGKHLGTIGNAGTFSFDFVKTITCAEGGAVVTNNQTVYTNCDGFTDHGHDHK
                     GVDRGADLHPFIGYNFRISELHAAVGLAQIKRLSTFLHIQRNNHAALKNILQQVPEVS
                     FREIPDPNGDSCTFLSWFLPTEEITKAFVAEMKAQGILAGNFYWYANNWHYIKQWQHL
                     QEAKSLYNYNPQQKDALLKLPSQNFSDSDAILSCCISTAISLVWINEQIIEKGTKMVA
                     AINKALELIDRVAPREKLYIRGLKAVIDGQAETRIEIFKELT"
     gene            1443..>2332
                     /gene="rsgA"
                     /locus_tag="PLY81_09655"
     CDS             1443..>2332
                     /gene="rsgA"
                     /locus_tag="PLY81_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNF30533.1"
                     /translation="MKALVYKSTGSWYIVKNSFGKQYNARIKGVLKIDGITSTNPIAV
                     GDEVIIEIENEIENTATIIEVCTRKNYVARQSPHNKRQHHIIASNLAQSLLFATITNP
                     VTSTGFIDRFLCSCEMFHIPAVIVFNKSDLYKEKDVEKFEQLKNIYTTVGYKVILTSL
                     INGNGIEEIKNILSNKTTLLSGHSGVGKSSFLNIVFPELNLRTQEVSSWSGKGLHTTT
                     FAEMFDIKDEGRIIDTPGIRELGLTGITKQELSGYFPEIKAEISHCKFNNCMHINEPA
                     CAVKNALQQGKIFEERYINYL"
BASE COUNT          780 a          391 c          358 g          803 t
ORIGIN      
        1 tgttaattct ttaaagattt caattctagt ttctgcttgt ccgtcaataa cagcttttaa
       61 tccacgaata tataattttt ctctaggcgc aactctatca attaactcta aagctttatt
      121 tattgctgct accattttag tacctttttc aattatttgc tcattgatcc atactaaact
      181 tatagctgta gatatacaac aacttaaaat tgcatcacta tccgaaaaat tttgtgatgg
      241 taatttaagt aaagcgtctt tttgttgtgg attatagttg tataaacttt ttgcttcttg
      301 taaatgctgc cattgtttaa tatagtgcca gttgttggca taccagtaaa aattacctgc
      361 taaaattcct tgtgctttca tttctgctac aaaggctttt gtaatttctt cggttggtaa
      421 aaaccaactt aaaaaggtgc aactatcgcc attagggtct ggtatttctc taaaactaac
      481 ttcgggtact tgttgtaaaa tattttttaa tgctgcatgg ttattacgct gaatatgtaa
      541 aaaagtagaa agccttttta tttgtgccaa tccaacagca gcatgtaact cactaattct
      601 aaaattatat ccaataaatg gatgtaggtc tgcacctctg tcaactcctt tatggtcatg
      661 tccatgatca gtaaaaccat cacaattagt ataaactgtt tggttgtttg ttactacagc
      721 acctccttcg gcacaagtaa ttgtttttac aaaatcaaaa ctaaaagtac cagcattgcc
      781 aattgtgcct aagtgtttac ctttatatgt tgcaccaata ctttggcagg catcttctaa
      841 taaaaataaa ttatgttcct tacaaattgc ttgtagtgca tctaaatctg ccatgctgcc
      901 acacatgtgt acaggcatta tacattttgt atttgcagtt atggcatttt ttacagcctt
      961 tggatttaag gttaaagttt tatctacatc tactaaaact ggaacagcac caacacttaa
     1021 tactgcttca aaacttgcca caaaagtaaa gcttggcatt ataacttcat ctcctgcacc
     1081 aatacctaat gcagccaaag cagtagttaa tgctgctgtg ccactacttg ttagttgtgc
     1141 atatttacaa ccaaaggttt cagtaatggc cttttctaat tctatacttt tccatattcc
     1201 ttttcttggt gtatcaaaac cataacgcat taatattcct gtttctaata catcatttac
     1261 atgttttttc tcttcttctc cccaaagttc ataacctggc ataatgttgt aatttatttt
     1321 tttataaaga gcaacaaaat tagagaaaca aagcaactaa aagcaatagt ttttttttag
     1381 caattcattt tacttttaaa caaataaaaa attaattact gtacttatta acttcgtaca
     1441 caatgaaagc ccttgtttat aaatctacag gaagttggta tattgtaaaa aatagttttg
     1501 gcaagcaata caacgcacgg ataaaagggg tattaaaaat tgatggcatt acatctacca
     1561 atcctattgc agttggagat gaagtgataa ttgaaattga aaatgaaata gaaaatactg
     1621 caacaataat tgaagtgtgc actagaaaaa attatgtggc tcgccaatct cctcataata
     1681 aaaggcaaca tcatattatt gcatctaact tagctcaaag cttacttttt gctactataa
     1741 ctaatcctgt aacttcaact gggtttatag ataggttttt atgctcctgc gaaatgtttc
     1801 acatacctgc tgtaatagtt tttaataaat cagacttata caaggaaaaa gatgttgaaa
     1861 aatttgagca actaaaaaat atttatacaa cagtagggta taaggtaata cttacaagtt
     1921 taattaatgg caatggtatt gaagaaataa aaaatatttt aagtaataaa acaacattgt
     1981 taagtgggca tagcggcgtt ggcaaatctt cttttttaaa tattgttttt cctgaattaa
     2041 atttaaggac acaagaggtt agcagttgga gtggtaaagg cttacacaca actacatttg
     2101 ccgaaatgtt tgacattaaa gacgaaggta gaattattga tacgccaggt attagagagc
     2161 ttggcttaac tggtattacc aaacaggaat tgagtggcta ttttccagaa attaaagcag
     2221 aaattagcca ctgtaagttt aataattgca tgcatataaa cgagccagct tgtgctgtaa
     2281 aaaatgcatt acaacagggc aaaatttttg aggaaaggta tattaactac ct
//
LOCUS       DAOQHP010000353         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_34224_length_2312_cov_4.324324, whole genome shotgun sequence.
ACCESSION   DAOQHP010000353 DAOQHP010000000
VERSION     DAOQHP010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34224_length_2312_cov_4.324324"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLY81_09660"
     CDS             complement(<1..414)
                     /locus_tag="PLY81_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HNF30534.1"
                     /translation="MERFQGLIGIVLILGIAFLFSNNKKRINYRLVISGILLQVAIAL
                     LIFKVPPVTRFFQMLGHGMEKIEKFAREGASFVFGGIMTQQYDGTAANYVKGGFIFAF
                     NVTATIILVCVLVAIFYHAGIMQRIVAIIAKAMNFI"
     gene            588..1892
                     /locus_tag="PLY81_09665"
     CDS             588..1892
                     /locus_tag="PLY81_09665"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNF30535.1"
                     /translation="MTFEKLLQQSLVWRGLYFFSLLLVNIFLSRSLQADGTGWIYYLT
                     NFFSFILLVASLSMESGYTYYAAGNIISHHKLAWFSLCWTLLICIISAAGVGIYFKSI
                     KGIDTFYTDNFIYYAATYISGILLINFFMVLFYAQKNYATPNILLSVINFLLIIIILL
                     NPKSYNIVVNSYFYSILTQGILLAIAFIYLNKSFQLISLPDKSSLKLLLNYSLVALTS
                     NVIFFFVYRIDYWFVKYNVESCTPDDLGNYIQASKLGQMMLIVPQIFASVIFPHTASG
                     ASKNNITESIHIFFRIFLQVLIVALIVVSIIGNWLFPAVFGITFNKMTMPVLLLLPGI
                     FSLSVLCLLSAYFGGVGKVKINVIGGVYALIIVIIGNLIFIPLYGIIGAAIVSSIGYS
                     VNLVYSFYHFFKDNPTSIKTLFIFKRSDWVWVKKMLFHKSTT"
     gene            1892..>2312
                     /locus_tag="PLY81_09670"
     CDS             1892..>2312
                     /locus_tag="PLY81_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30536.1"
                     /translation="MKIIWLASWYPNKYEPVNGDFIQRHAKAVSNILPIDVLHVVQLG
                     KNFEANNEIETINAKNLTEHICYFKFKKWDIGIIDKIRYNTLYKKTALAFVENFIKTN
                     GKPDLIHVHVPMKSGWVAYSLKKKYSIPYIVSEQASYY"
BASE COUNT          778 a          287 c          339 g          908 t
ORIGIN      
        1 aataaaattc attgctttag ctataattgc aacaatacgt tgcataatac ctgcatggta
       61 aaatattgca accaatacac aaactagaat aatagtagca gtaacattaa aggcaaaaat
      121 aaaaccgcct tttacatagt tggctgctgt accatcgtac tgttgagtca ttataccacc
      181 aaatacaaaa gatgctcctt ctcttgcaaa cttttcaatt ttttccattc catgacctaa
      241 catttgaaaa aatctggtaa ctggtggaac tttaaaaatt agtaatgcta ttgcaacctg
      301 aagtaaaata ccactaataa ctaaacgata attaattctt tttttgttgt tggagaataa
      361 aaaagcaatt cctaaaataa gtactatacc tattaagcct tgaaatcttt ccataagaaa
      421 tgttggttgt tagttttgtt ttaataagat tgaaagataa gataaaataa aatatcaaaa
      481 tgaaaatttt tatataatac tatcaattaa aaaattttga aaatgtatac ttcaacttta
      541 tcaattaaac tattgtacat tagctgcttt aaataagtac tgcttacatg acttttgaaa
      601 aattattgca acagagttta gtgtggcgtg ggttatactt tttttcttta ttattagtaa
      661 atattttttt aagcagaagc ttacaagcag atggcacagg ttggatatat tatttaacta
      721 actttttttc ttttatttta ttggttgcaa gtttaagtat ggaaagtgga tatacttact
      781 atgctgctgg caatattatc agccatcata aacttgcctg gttttcgttg tgttggacct
      841 tattaatttg tattatttca gcagcaggtg taggtattta ttttaaaagt ataaaaggaa
      901 ttgatacttt ttataccgat aattttattt attatgctgc tacttatatt tcgggcattt
      961 tgctaattaa tttttttatg gttttatttt acgctcaaaa aaattatgct acgccaaata
     1021 ttttattaag tgttataaac tttttgctca ttataataat tttattgaat ccaaaatcgt
     1081 acaatattgt agttaatagc tatttctaca gcatattaac acaaggtatt ttattggcta
     1141 ttgcatttat ttatttgaat aaatcttttc aactaatttc tttgccagat aaatcgtcat
     1201 taaaattatt gttgaattac tctttagtag ctttaactag taatgtaatt ttcttttttg
     1261 tatatagaat agattattgg tttgtaaaat acaatgttga aagttgtaca ccagatgatt
     1321 taggtaatta tattcaagca tcaaagcttg gacaaatgat gttaattgtg ccacaaattt
     1381 ttgcaagtgt tattttccca catacagcca gtggagcttc caaaaataat attacagaat
     1441 ctatccatat ttttttcaga atatttttac aagtattaat tgttgctctt attgttgtaa
     1501 gtattattgg aaactggctt ttccctgctg tgtttggcat tacttttaat aaaatgacta
     1561 tgcctgtttt attattactt cctggtatat tttctttatc tgttttatgt ttattgagtg
     1621 cttattttgg tggtgttggt aaagttaaaa ttaatgttat tggtggggtt tatgctttaa
     1681 ttatagtaat aataggaaat ttaattttta ttcctttata tggaattatt ggtgctgcca
     1741 ttgtaagcag tattggctat tctgttaatt tagtatattc tttttatcac ttttttaaag
     1801 ataatcctac aagtataaaa acgttattta tttttaaaag aagcgattgg gtgtgggtga
     1861 agaaaatgct ttttcataaa tctactacat aatgaaaatt atttggcttg caagttggta
     1921 tcctaataaa tatgaaccag taaatggcga ttttattcaa cgccatgcaa aagctgtttc
     1981 taatatatta cctatagatg tattgcatgt agtgcaatta ggaaaaaatt ttgaggctaa
     2041 taatgaaatt gaaactatta atgcaaaaaa ccttacagaa catatatgtt attttaagtt
     2101 taaaaaatgg gacattggta taattgataa aataagatac aatactttat ataagaaaac
     2161 ggctttagct tttgttgaaa attttattaa aacaaatggt aagcctgatt taatacatgt
     2221 tcatgtgccc atgaaatctg gttgggtagc atattcattg aaaaaaaaat attccattcc
     2281 atatattgtg tctgagcagg cttcttatta tg
//
LOCUS       DAOQHP010000354         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_34226_length_2312_cov_3.435091, whole genome shotgun sequence.
ACCESSION   DAOQHP010000354 DAOQHP010000000
VERSION     DAOQHP010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34226_length_2312_cov_3.435091"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLY81_09675"
     CDS             complement(<1..290)
                     /locus_tag="PLY81_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNF30537.1"
                     /translation="MEKEKIKNIFIEGAITAEFIGNSIYKHSTKKNIGAHSIFLGQVR
                     NDIIKEQQVKAIEYTAYTEMANEKFYEIREDAFKKYPLVCMHIYHSLGIVNAG"
     gene            complement(302..1426)
                     /locus_tag="PLY81_09680"
     CDS             complement(302..1426)
                     /locus_tag="PLY81_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNF30538.1"
                     /translation="MNNHLYDRYQRQILLKEFGTEAQAKLFNAKVLVIGAGGLGCPAL
                     QYLAAAGVGTIGIVDFDLIELSNLQRQTLYNTNDIGKHKTTVSAERINAFNPSIHIQT
                     HTIQLNNTNAYNLIYNYDIVIDGTDNFSTKYLINDVCTILQKPLIYGAILRFEGQVSV
                     FNLPNKQNICTNYRDLFATPPLPNNVPTCNEAGVIGVLPGIIGTMQAAEAIKIITNIG
                     EPLCNKILSYNVLNNKSYLFDILPITHHSNNFPTTKTEIENFNYDWHCGLNDTVTEIE
                     FYEFEELRKNEEIKIIDVREEGEIPKVEAFSCINIPLSNFVNKIQNIELTNRVVVFCQ
                     HGKRSIKAAQILKAKYNHLNIVSLKGGIVEWEKQFNDSIY"
     gene            complement(1428..1661)
                     /locus_tag="PLY81_09685"
     CDS             complement(1428..1661)
                     /locus_tag="PLY81_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNF30539.1"
                     /translation="MQINVLAFGQIAEAMNGESFTFSNINTTNDLIDYLKQQYASLKT
                     LDYKIAVNKQIIHSNMPLNNHDTVALLPPFSGG"
     gene            complement(1651..2247)
                     /locus_tag="PLY81_09690"
     CDS             complement(1651..2247)
                     /locus_tag="PLY81_09690"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNF30540.1"
                     /translation="MEKFKHIDAFVLAGGKSSRMGTDKGLLLFENKHLVEWAIDELQL
                     IFDKVIIVSNKQVYKKFKLEVIKDVKKNLGPAGGIYSILKHTKTNYVVVVSCDMPFIN
                     SNAIAFLINQCNDYDITIPVYKNKLQPLCAVYSKNCLVKWKELIDKNEIKLQNMIEHF
                     NVLKINVDDNSLFNESQFVNINDRNDFENAFQLLKYAN"
BASE COUNT          760 a          379 c          280 g          893 t
ORIGIN      
        1 ccagcattta caatgcccag gctatgataa atgtgcatac aaaccagtgg atatttttta
       61 aaagcatctt cacgtatttc ataaaatttt tcatttgcca tttctgtata tgcagtgtac
      121 tctatagctt ttacctgttg ctccttaatt atatcatttc taacctgacc taaaaaaata
      181 ctgtgtgcac caatattttt tttagtgcta tgtttataaa tagaattgcc aataaactcc
      241 gcagtaattg caccctctat aaaaatattt tttatttttt ctttttccat tgttatctca
      301 tttaataaat actatcatta aactgttttt cccattctac tattccgcct tttaaactaa
      361 caatatttag gtgattatat tttgctttta atatttgtgc tgctttaata cttcttttgc
      421 catgctggca aaatacaaca accctatttg taagttcaat attttgaatt ttatttacaa
      481 aattacttaa tggaatattt atacaactaa acgcttctac ttttggtatt tcaccttctt
      541 ctctaacatc aataattttt atttcttcat tttttctcaa ctcttcaaat tcataaaact
      601 ctatttctgt tacagtatca tttaacccac aatgccaatc gtaattaaaa ttttctatct
      661 ctgttttagt ggtaggaaag ttattactat ggtgtgtaat tggtaaaata tcgaataaat
      721 aacttttatt gttgagtaca ttatacgata aaattttatt gcacaaaggc tctccaatat
      781 tggtaataat tttaatagct tcggcagcct gcattgttcc aattattcct ggcagcacac
      841 caattactcc agcctcgtta cacgtaggaa cattattggg caatggtggg gtagcaaata
      901 aatctctata attggtgcat atattttgtt tattaggtaa attaaaaaca cttacctgcc
      961 cttcaaatcg tagaatagca ccatatatta aaggtttttg cagtattgta caaacatcat
     1021 ttatcaaata cttagtagaa aaattgtctg taccatcaat tacaatatca taattataaa
     1081 taaggttata ggcgttagta ttatttaatt gtatggtatg tgtttgtata tgaatagaag
     1141 gattaaacgc attaattctt tctgctgaaa cagtagtttt atgtttgcca atatcatttg
     1201 tattatacaa tgtttgcctt tgtaagttgg aaagctctat caaatcaaaa tcaacaatac
     1261 caattgtacc aacaccagca gcagcaagat attgcaatgc agggcaaccc aagcctcctg
     1321 caccaattac taatacttta gcattaaaaa gtttagcttg tgcttctgtt ccaaattctt
     1381 ttaataaaat ttgtcgttgg tatctatcgt ataaatgatt attcatatta acctcctgaa
     1441 aagggtggta ataaagctac agtatcgtgg ttgtttagtg gcatatttga atgaataatt
     1501 tgtttattca cagcaatttt atagtctaat gtttttaagg acgcatattg ttgctttaaa
     1561 taatctatca aatcattagt ggtatttata ttgctgaaag taaaactttc tccattcatt
     1621 gcttctgcaa tttgtccaaa ggctaataca ttaatttgca tattttaata attgaaatgc
     1681 attttcaaaa tcgtttctat cgttaatatt tacaaattgc gactcattaa aaagagaatt
     1741 atcatccaca ttaattttta gtacattaaa atgttcaatc atgttttgca atttaatttc
     1801 attcttatca atcaattctt tccattttac taaacaattt ttagaatata ctgcacacaa
     1861 aggttgtaat ttatttttat atacaggtat tgtaatatcg tagtcattgc attgattaat
     1921 taaaaatgct atagcattag aattaataaa tggcatatcg caacttacaa caactacata
     1981 atttgtttta gtatgtttta aaatggaata aataccaccc gccggaccaa gatttttttt
     2041 aacatcttta attacctcta acttaaattt tttatacact tgtttattgg aaactataat
     2101 tactttatca aaaattaatt gcaattcatc aattgcccat tcaaccaaat gtttattttc
     2161 aaataagagc aaacccttgt ctgttcccat tcttgagctt ttgccacctg caagcacaaa
     2221 tgcatctata tgtttaaatt tttccataaa ctacgtaaac aataatttta tatgaagcca
     2281 gtaataacac tgtagctaat aaagttttta aa
//
LOCUS       DAOQHP010000355         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_34277_length_2310_cov_2.941020, whole genome shotgun sequence.
ACCESSION   DAOQHP010000355 DAOQHP010000000
VERSION     DAOQHP010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34277_length_2310_cov_2.941020"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLY81_09695"
     CDS             <1..761
                     /locus_tag="PLY81_09695"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNF30541.1"
                     /translation="SKAMTKVFKEDVKISVYLRTMNKDTISQIQQFISNQPYAKDVHY
                     IDKEQAKKIWNAENNEDWAQFLDVNPLPESIDFFAKADYVNKDSLEVIQKSINTAYQY
                     QITEVNYPPVLVNNLDAKARQIGLILLVVAIILAVVVIISIDNTIKLAMFSNRFLIKT
                     MQMVGATRSFIARPMNIRAVVNGLISGAIAVVLLLTIILWAESYFPELKKIRDLQLTL
                     TLFGGMILLGIAISVYSTHRSILKYLKMKLDDLY"
     gene            881..2056
                     /locus_tag="PLY81_09700"
     CDS             881..2056
                     /locus_tag="PLY81_09700"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNF30542.1"
                     /translation="MQEAYIVAGYRTAVTKSKKGGFRFYRPDDLAVEVIKALKATIPQ
                     LESNRIDDVIVGNAVPEAEQGLQFGRIIAAKALGIEVPGITINRYCASGLESIAMATA
                     KIRTGMAECIIAGGTESMSLVPTAGWKTVPSYAIAKDEPDYYLSMGLTAEAVAKEYNV
                     NREQQDEFAYHSHRKAAIAINEGYFKSGILPINVEEVYLNEKGKKASRNYVVDTDEGL
                     RADTTIEGLAKLKPAFSQGGTVTAGNSSQTSDGAAFVIVMSEKMVKELNVQPIGRLVN
                     CASAGVHPRIMGVGPIAAIPKVLKQANMQLNNIDLIELNEAFASQSIAVINNLGLNND
                     IVNINGGAIALGHPLGCTGAKLTIQILNDMKRLNKKFGLVTACVGGGQGIAGIIENF"
     gene            complement(2061..2213)
                     /locus_tag="PLY81_09705"
     CDS             complement(2061..2213)
                     /locus_tag="PLY81_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30543.1"
                     /translation="MAKEKKSFLQEAVTNEYILMFEHDVHNECCTLQQTERGIKVNEI
                     FKLSEI"
BASE COUNT          819 a          311 c          438 g          742 t
ORIGIN      
        1 atagtaaagc aatgacgaag gtttttaaag aagatgtaaa aataagtgtg tatctaagaa
       61 caatgaataa agataccata agccaaattc aacagtttat aagtaatcaa ccctatgcta
      121 aagatgttca ttatattgat aaagaacaag ctaaaaaaat atggaatgca gaaaataatg
      181 aagactgggc tcaattttta gatgtaaacc cacttccaga aagtatagat ttttttgcta
      241 aggcagatta tgtaaataaa gatagtttag aggtaattca aaaaagcatt aatacagcct
      301 atcaatatca aattacagaa gttaactatc cgccagtatt agtaaataat ttagatgcca
      361 aagcaaggca aattggttta atattattgg ttgtggcaat tatattagct gttgtagtaa
      421 ttattagtat tgataataca ataaaacttg ccatgtttag caatagattt ttaattaaaa
      481 ccatgcaaat ggtaggtgcc accagaagtt ttattgccag gccaatgaat attagagcag
      541 tagttaatgg attaattagt ggtgcaatag cagtagtttt attgctaaca attattcttt
      601 gggcagaatc ttactttcct gaattgaaaa aaattagaga cttacagctt accttaactc
      661 tttttggtgg aatgatttta ttaggtattg caattagtgt ttatagcacc catcgtagta
      721 ttttaaaata cttaaaaatg aagctagacg atttatatta attcaatctt gctaatatta
      781 tcaatatttt tccactttat gtaccatatt gttttgtaac aaaaattaaa ttggttgcat
      841 aaacaacttt ctttacattt ggattttaaa attgtatttt atgcaagaag catatatagt
      901 tgcaggctat agaactgctg ttacaaaaag taagaaagga ggttttcgtt tttaccgacc
      961 tgatgattta gctgtagaag ttataaaagc tttaaaagct actatacctc agttagagtc
     1021 aaatagaata gatgatgtaa ttgtaggaaa tgcagtaccc gaagcagaac aagggctaca
     1081 gtttggaaga ataatagcag caaaagcttt aggtattgaa gtgccaggta ttacaattaa
     1141 tagatattgt gcaagtggat tagaaagtat tgcaatggct actgcaaaaa ttagaacagg
     1201 tatggcagag tgtattatag ctggtggcac agaaagtatg agccttgtac caacagcagg
     1261 ctggaaaaca gtaccctctt atgctattgc aaaagatgaa cctgattatt atttaagcat
     1321 gggtttaact gccgaagcag ttgcaaaaga atataatgtt aatagagaac aacaagatga
     1381 gtttgcttac cattctcaca gaaaagctgc tatagcaata aatgaaggct attttaaatc
     1441 gggtatttta ccaataaatg tggaggaagt ttatttgaac gagaaaggta aaaaggcttc
     1501 gagaaattat gttgttgata ccgatgaagg cttaagagca gataccacta tagaaggttt
     1561 ggcaaaacta aaacctgctt tctctcaagg gggcactgtt actgcaggca acagttctca
     1621 aactagtgat ggtgctgctt ttgtaattgt tatgagtgag aaaatggtga aagagctaaa
     1681 tgtacaacct attggcaggt tagtaaattg tgcaagtgca ggtgtacacc ctcgtattat
     1741 gggtgtgggg ccaattgctg caataccaaa ggttttaaaa caagccaata tgcaattgaa
     1801 taatattgat ttaatagaat taaacgaagc atttgcgtcg caaagcattg ctgttattaa
     1861 taatttgggc ttaaataatg atattgtaaa tattaatgga ggtgctattg cattagggca
     1921 tccactaggt tgcacaggag caaaactcac tattcaaatt ttaaatgaca tgaagcgact
     1981 aaacaaaaaa tttggcttag ttactgcttg tgttggagga gggcaaggga ttgccggaat
     2041 tattgaaaat ttttaaaagg ttatatttcg ctaagtttaa atatttcatt cacttttatt
     2101 cctctttctg tttgttgtaa ggtacagcat tcgttgtgta catcatgctc aaacattaaa
     2161 atatattcgt ttgtaacagc ctcttgtaaa aacgattttt tttcttttgc catagattta
     2221 ttaattaaat aattaccaaa tttcttttat tttttttact ctttcgaaac cggcgattag
     2281 aattatgagc gcaatccaga ctacagagaa
//
LOCUS       DAOQHP010000356         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_34396_length_2306_cov_2.343403, whole genome shotgun sequence.
ACCESSION   DAOQHP010000356 DAOQHP010000000
VERSION     DAOQHP010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34396_length_2306_cov_2.343403"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLY81_09710"
     CDS             <1..860
                     /locus_tag="PLY81_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNF30544.1"
                     /translation="NEINNKFICFNGLFISNKTAIVDASNRGLKYGDGFFETLRIINN
                     KIVLKEFHFNRLFDALQSLHFEQPNFLTSDFLEEKILNLIQKNNFTTAAKVRINIFRG
                     NGNLFEIENNYPNYIIEAEPLPNQFYEWDKTGLKVDFFIDAKKVCDNFSHLKTNNFLP
                     YSMAAFWAKKNNLHSAIILNQHNCVADSAIANVWVIKNGEIQTPALTQGCIDGVMRKY
                     LIKCFKEEGLPFKETALTIEDIVQAQEVFLTNVIKGISWVKFCGKTEYTNTTTQFIYS
                     KFVHTLNSY"
     gene            complement(847..1659)
                     /locus_tag="PLY81_09715"
     CDS             complement(847..1659)
                     /locus_tag="PLY81_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNF30545.1"
                     /translation="MKLTLGFSPCPNDTFIFDALVNNKIDTNGFTFNVVLSDVQTLNQ
                     WALEEKLDVTKISYGVYPLLLEKYKLLNSGGALGNGVGPLLITNSTALENNTINANSS
                     VAIPGINTTAHLLFSMAYPNHANKQFMVFNEIEDAVLNGKVDAGVIIHENRFTYQQKG
                     LKKIIDLGEYWEEKLKIPIPLGGIVAHKRVEESILSTVDELIKKSIEFAFANYPSITD
                     YVKQHAQEMDESIMKQHIDLYVNNYSLDIGNDGKKAVEKLMDIHHLLSTNKS"
     gene            complement(1671..>2306)
                     /gene="mqnB"
                     /locus_tag="PLY81_09720"
     CDS             complement(1671..>2306)
                     /gene="mqnB"
                     /locus_tag="PLY81_09720"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNF30546.1"
                     /translation="EWKPAFLNINKLYTKKNKRLNVSFHVSGVGIAATAVSLTKLLCN
                     EQPDLIIQMGIAGAFNNKEELGKTFAVKNDCFADMGVEENGEWKDIFDLKLNKNNCPP
                     YAKKGLPNPFLQQYNLLHLPVAKGITVNTINTQKRRIEQFKKKYAPDIETMEGAALHY
                     VCRDAKVAFLQIRSTSNYVGERNKTKWLLKESIENVNNTALKLIDKLYEIA"
BASE COUNT          781 a          350 c          323 g          852 t
ORIGIN      
        1 ctaatgaaat aaataataaa ttcatttgtt ttaatggatt gtttattagt aataaaactg
       61 ccattgtaga tgcatctaac aggggattga aatatggaga tggttttttt gaaactttga
      121 ggataataaa taacaaaatt gtactaaaag aatttcattt taataggtta tttgatgcat
      181 tgcagtctct tcattttgag caacctaatt ttttgacatc tgatttttta gaagaaaaaa
      241 ttttaaacct tattcaaaaa aataatttta ctacagcagc aaaagttaga ataaatattt
      301 ttagaggtaa tggaaatttg tttgagatag aaaataatta tcccaattat ataatagagg
      361 cagaaccact accaaaccag ttttatgaat gggataaaac tgggttgaaa gttgattttt
      421 ttatagatgc aaaaaaagtt tgcgataatt tttctcatct taaaaccaat aattttttac
      481 cttattcaat ggcagcattt tgggcaaaaa aaaataatct acattcagca atcatcttaa
      541 atcaacataa ctgtgttgca gattctgcaa ttgccaatgt ttgggtaatt aaaaatggag
      601 aaatacaaac tcctgcatta actcaaggat gtattgatgg tgtaatgaga aaatatttaa
      661 ttaaatgttt taaagaagaa ggattacctt ttaaagaaac tgcattaacc attgaggaca
      721 tagtacaagc acaagaagta tttttaacta atgttattaa aggcattagt tgggttaagt
      781 tttgtggtaa aaccgaatat accaatacca caactcaatt tatttattca aaatttgtac
      841 atacactcaa ctcttattag tagatagcaa atggtggata tccattaatt tttcaactgc
      901 ttttttacca tcatttccaa tatctaaaga atagttattt acatacaagt ctatatgttg
      961 cttcataatg gattcatcca tttcctgagc atgttgcttt acataatctg taatagaagg
     1021 atagtttgca aaagcaaatt caatactttt tttaattagt tcatctactg tagaaagaat
     1081 gctttcttct actcttttat gtgcaacaat accacctaac ggaattggaa tttttaattt
     1141 ttcttcccag tattcgccta agtcaattat tttttttaat cctttttgtt gataggtaaa
     1201 tctgttttca tgaataataa caccagcatc cactttccca tttaacacag catcttcaat
     1261 ttcattaaac accataaatt gtttattagc atggttaggg taagccatgg aaaataataa
     1321 atgtgctgtt gtatttattc ctggaattgc aactgaagaa ttggcattaa tagtattgtt
     1381 ttctaaagct gttgaatttg taatgagcaa agggccaaca ccattgccta atgcaccacc
     1441 actattcaat aatttgtatt tttctaaaag tagaggatat acgccataac taattttcgt
     1501 tacatctaac ttttcttcta aagcccattg gttcaaggtt tgaacatcgg ataatacaac
     1561 attaaaagta aaaccatttg tatcaatttt attatttacc agtgcatcaa aaataaatgt
     1621 atcgtttggg caaggcgaaa agccaagtgt tagtttcatc tacaaaatgt ttaagcaatt
     1681 tcgtacaatt tgtcaatcaa ttttaaagca gtattattta cattttcaat actttctttt
     1741 aaaagccatt ttgttttatt gcgttcgcca acataattac ttgttgatct tatttgcaaa
     1801 aaagcaacct ttgcatctct gcaaacataa tgaagtgctg ctccttccat tgtttcaata
     1861 tctggagcat actttttttt aaattgttca attcttcttt tttgtgtgtt aattgtattc
     1921 actgttatac cttttgctac gggcaaatgt agcaaattgt attgttgcaa gaatgggtta
     1981 ggcaatcctt tcttggcata aggtgggcaa ttatttttat ttaatttcaa atcaaaaata
     2041 tctttccatt ctccattttc ttctacaccc atatcagcaa aacaatcatt ctttactgca
     2101 aaggtttttc ctaattcttc tttattatta aatgctccag caatacccat ttgaatgatt
     2161 aagtctggtt gttcattgca caataatttt gtaagtgaaa cagcagtagc agcaatacct
     2221 acaccactta catgaaacga aacatttaat cttttgtttt ttttagtata aagtttatta
     2281 atgtttaaaa atgcaggctt ccattc
//
LOCUS       DAOQHP010000357         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_34824_length_2289_cov_3.909579, whole genome shotgun sequence.
ACCESSION   DAOQHP010000357 DAOQHP010000000
VERSION     DAOQHP010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34824_length_2289_cov_3.909579"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /gene="feoB"
                     /locus_tag="PLY81_09725"
     CDS             complement(<1..1942)
                     /gene="feoB"
                     /locus_tag="PLY81_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNF30547.1"
                     /translation="MQQNFNIVIVGNPNSGKSTLFNTLTGLNQKTGNFPGVTVDKKSG
                     KIELRNDIHANLIDLPGTYSLYPRRADEWVAYKVLMNADEYIKPDVVLLVVDASNLKR
                     NLLFCSQILDTKTPVVIALTMNDLAAKKGIKIDIEGLQTDLGVPVVEVNPRKSKGTNE
                     LKKILLLLTKQAPVNNRDFIDNKTLAPQSIEAIQALQPNINTYTAIHYLINYQQLNVD
                     NSFKQQTATIIQSTNFNATKVQAEEVLQRYQRIRGLMQKNVFAPDPAEKKLITDKLDN
                     VLLHRKWGYLILLAVLFILFQSIFWLAQYPMNLIESSFASITSFFNHILPSTWYTDLF
                     INGIIAGLGGIVIFVPQIMILFGLITILEDTGYMARISFLSDKLMRKVGLNGKSVMPM
                     ISGLACAVPAIMSTRNIENRKERLLTILVTPLMSCSARLPVYTILIALAIPEKYYFGF
                     LSLQGLVMMALYLFGTFMALLVSFVMKWFIKINEKSFFILELPIYRNPRWKNVGLTMI
                     EKSKIFVVEAGKVIMVISLLLWFLSSYGPGDEMKKIDLHYEQLILQNPTQSDSLQKIA
                     STEKLEHSYAGLLGKKIEPIIKPIGYDWKIGIALITSFAAREVFVGTMATLYSVEESD
                     DSSLREKMQAATHRNGTKVYTLA"
     gene            2214..2286
                     /locus_tag="PLY81_09730"
     tRNA            2214..2286
                     /locus_tag="PLY81_09730"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2247..2249,aa:Lys,seq:ttt)
BASE COUNT          742 a          379 c          338 g          830 t
ORIGIN      
        1 ttgcaagggt ataaacttta gtgccatttc tatgggtggc agcttgcatt ttttctctta
       61 aggatgagtc gtcgctttct tccacactat ataaagttgc cattgtacct acaaatactt
      121 ctcttgctgc aaatgaagta attaaggcta taccaatttt ccaatcataa ccaattggtt
      181 ttataattgg ttctattttt ttacctaata accctgcata agagtgttct aatttttcgg
      241 tggaggcaat tttttgcaaa gaatcggatt gtgttgggtt ttgtaaaatt aattgttcat
      301 aatgcaaatc aatttttttc atttcatctc cagggccata gctgcttaaa aaccataata
      361 gtaagctaat taccattatt accttacctg cttctaccac aaatatttta gatttttcaa
      421 tcattgttaa tccaacattt ttccatcttg gattacgata aattggtagt tctaaaataa
      481 aaaagctttt ctcgtttatt ttaatgaacc acttcataac aaagcttacc aacaatgcca
      541 taaaagtgcc aaataaatac aatgccatca ttactaaacc ttgtaaactt aaaaaaccaa
      601 agtagtattt ttccggaatt gctaaagcaa ttaatatagt ataaacaggt aatcttgcac
      661 tacaactcat taaaggtgta actaaaatag tcagtagcct ttcttttcta ttttcaatat
      721 ttcttgtact cataatggct ggcactgcac aagccaaacc actaatcatt ggcattacac
      781 tttttccatt taatccaact ttacgcatta gtttatcgct taaaaaacta attcttgcca
      841 tgtagccggt atcttctaaa atagttatta aaccaaacaa aatcataatt tgtggaacaa
      901 aaattactat tccacctagc cctgcaataa ttccattaat gaataaatcg gtgtaccaag
      961 ttgacggtaa aatatgatta aagaaacttg ttatacttgc aaaagaagat tcaattaaat
     1021 tcattgggta ttgagctaac caaaatatac tttgaaaaag aataaacagc actgccaata
     1081 aaattaaata tccccattta cgatgaagta aaacattatc taacttatct gttattaatt
     1141 ttttttctgc ggggtcgggg gcaaaaacat ttttttgcat taagcctctt atacgttgat
     1201 aacgttgtaa aacctcctca gcctgcactt tagttgcatt aaagttggta gattgtatga
     1261 tagttgcagt ttgctgttta aatgaattat ctacatttaa ctgttggtaa tttattaaat
     1321 aatgaattgc ggtataagta ttaatattgg gttgcagggc ttgtatagct tcaatacttt
     1381 gtggtgctaa ggttttatta tctataaaat ctctgttgtt tacaggtgct tgtttagtta
     1441 atagtagcaa aatttttttc agttcattag tacccttgct ctttcttgga ttaacttcta
     1501 ccactggtac acctaaatct gtttgtaagc cttcaatatc tattttaata ccttttttag
     1561 cagccaaatc gttcatggtt aatgctatta caactggtgt ttttgtatct agtatttggc
     1621 tacaaaacaa taaatttcgc ttaaggttac tagcatctac taccaataaa acaacatctg
     1681 gtttaatgta ttcatcggca ttcattaaaa ctttataagc tacccactcg tctgctcttc
     1741 ttgggtataa tgagtaggtg cctggcaaat ctataaggtt ggcatgtata tcatttctta
     1801 attctatttt acccgatttt ttatcaacag taacaccagg aaagttgcct gttttttgat
     1861 ttagccctgt taatgtatta aataaggtac tttttccgct gttaggatta ccaacaataa
     1921 ctatattaaa attttgttgc aatgagtata tttataagat ggcgaatgta atagcagcat
     1981 ttttttgtta ttatcaaaat caggaattat tatgttaagg ctgctattct atacagcaaa
     2041 ttaagtacaa taataaaaaa gaagtagaag tgtagaaatt ttctttaatg atattaatac
     2101 taataactac ggagttatca acaattattt ataaattagt tgttgaattt ttaaagaaaa
     2161 atttggttac aacattcaga acactatttt tgcactccca attaagaaag gtagattccg
     2221 tagctcagtt ggtagagcaa tacactttta atgtatgggc cctgggttcg agtcccagcg
     2281 ggatcacaa
//
LOCUS       DAOQHP010000358         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_35049_length_2281_cov_3.724169, whole genome shotgun sequence.
ACCESSION   DAOQHP010000358 DAOQHP010000000
VERSION     DAOQHP010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35049_length_2281_cov_3.724169"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLY81_09735"
     CDS             <1..320
                     /locus_tag="PLY81_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30548.1"
                     /translation="FKETKRNYPIEMPYTISQTYLFSMETPKGYEIAEMPKSARILLN
                     ETEGLFEYLIAKKSNGSIQLKCVVKINVANFNAAEDFETLKGFYNFIIQKESEPIVFK
                     KKK"
     gene            complement(326..1237)
                     /locus_tag="PLY81_09740"
     CDS             complement(326..1237)
                     /locus_tag="PLY81_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNF30549.1"
                     /translation="MIKVPSYLKKGDTIGLVCPAGYMPLNKATTCINTLQNWGYNVCI
                     GKTVGGKQKNYFSGTDVERLNDFQAMLDDDAIKAILCARGGYGVSRIIDQLNFTKFKK
                     NPKWIIGFSDITVFHAHINNQYNIATMHAPMAAAFNDNGFKNEYILSLKNALAGKKNK
                     YYCKPHILNNLGKINGELIGGNLALITHLIGSKSAYNTNKKILFIEDIGEYLYNIDRM
                     FVQLKRSGALNNLNALIIGGFSELKDTSKPFGQDVYNIIHFHVKDYNYPICFNFPVSH
                     NRENVTLKIGINYELNIQKNSVVLKEL"
     gene            1359..1850
                     /gene="rfaE2"
                     /locus_tag="PLY81_09745"
     CDS             1359..1850
                     /gene="rfaE2"
                     /locus_tag="PLY81_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNF30550.1"
                     /translation="MKQIAAIEKKIFDLPALVKQVAIWKKFNKTIAFTNGCFDILHDG
                     HIYSLSQAAKEADYLIVAVNSDSSIKKIKGEDRPINHQQSRAMMLASFAIIDAVIIFE
                     EDTPYNLITTLLPNVLVKGGDYTINQIIGAEEVIKNGGNVVINPIIQGFSTTGIINKI
                     KNL"
     gene            1989..>2281
                     /locus_tag="PLY81_09750"
     CDS             1989..>2281
                     /locus_tag="PLY81_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF30551.1"
                     /translation="MTEIEMIKGCIRQDATCQRLLYEQLSGKMFTVCLRYANDLMEAE
                     DLLQEGFIRVFSNVHQFKFEGSFEGWVRRIMVNSALKCIQKKNKTKNKTKNII"
BASE COUNT          872 a          311 c          322 g          776 t
ORIGIN      
        1 cgtttaaaga aactaaaaga aattatccaa tagaaatgcc ctatactata agtcaaactt
       61 atttattttc tatggaaact cctaaaggct atgaaattgc agaaatgcca aaaagtgcaa
      121 gaatattact taatgaaacc gaaggattgt ttgaatattt aattgccaaa aaatccaatg
      181 ggtcaataca acttaaatgt gttgtaaaaa ttaatgttgc caattttaat gcagcagaag
      241 attttgaaac cttaaaaggg ttttacaatt ttattattca aaaagaaagc gaaccaattg
      301 tgtttaaaaa gaaaaaataa aaccactaaa gttcttttaa aactacgctg tttttttgta
      361 tgtttaattc gtagtttata ccaattttca acgttacatt ttctctatta tggcttacag
      421 gaaaattaaa acaaattggg tagttatagt cttttacatg aaaatgaatg atattgtaaa
      481 catcctgtcc aaatggctta gaagtatctt ttagttcaga aaatccgcca attattaaag
      541 catttaagtt atttaatgca ccactgcgtt ttaattgtac aaacattcta tctatattat
      601 ataaatattc accaatatct tcaataaata atattttttt attggtgtta tatgcagact
      661 tggagccaat taaatgtgtt attaatgcca aatttccgcc aattaattca ccatttattt
      721 tacctaaatt atttaatata tgaggcttgc aatagtattt attcttttta cctgctaaag
      781 cattttttaa tgatagaata tattcattct taaatccatt atcattaaat gcagcagcca
      841 taggtgcatg catagttgca atattgtatt ggttgtttat gtgtgcatga aaaactgtta
      901 tatcgctaaa tccaataatc catttaggat tttttttaaa ttttgtaaaa tttaattggt
      961 caataattct gcttactcca taaccacctc ttgcacaaag aatggcttta atagcatcat
     1021 catctaacat tgcctgaaag tcatttaacc tttctacatc tgttccagaa aaataatttt
     1081 tttgtttacc accaacagtt tttccaatac aaacattata cccccagttt tgtaaagtat
     1141 taatgcaagt ggttgcttta tttaaaggca tatagcctgc tgggcaaact aaacctattg
     1201 tatcgccttt tttaagatac gaaggaactt taatcatata gcaatattac aaaccatact
     1261 caatatttgt atttaatata ctataaacac aaaattttct ctaaaatagg ttggtaattt
     1321 tatttacctt gcaatataca tttcaataaa tacattaaat gaaacaaatt gcagcaatag
     1381 aaaaaaaaat attcgattta ccagcattgg ttaaacaggt agccatttgg aaaaaattta
     1441 ataaaactat tgcttttact aatggttgtt ttgatatttt acacgatggg catatatact
     1501 ctttatcgca ggctgctaaa gaagcagatt atttaatagt tgcagttaat agcgatagta
     1561 gtataaaaaa aattaaggga gaagatagac caataaatca tcagcaaagt agagcaatga
     1621 tgttagcaag ttttgccatt attgatgctg taataatttt tgaagaagat acaccatata
     1681 atttaattac aaccttatta cctaatgtat tagtaaaagg tggagattat acaataaacc
     1741 aaattatagg tgccgaagaa gtaataaaaa atggagggaa tgttgttatt aatcccatca
     1801 ttcaaggttt ttcaacaacc ggaattataa ataaaattaa aaatttataa gataagttct
     1861 taataatcaa ctaaataatt tattagtggt agagtaataa aaaaaaatta actttatgca
     1921 ttcaaagcag cataaacaat tactgcagta tcactattta agtaaacata aaaaattaac
     1981 acactacttt gactgaaatt gaaatgataa aagggtgtat taggcaagat gccacttgtc
     2041 aacgattgtt atatgaacaa ttgagtggca aaatgtttac agtttgttta cgatatgcca
     2101 acgatttaat ggaggccgaa gatttgttgc aagaaggttt tataagagta tttagcaacg
     2161 tacaccagtt taagtttgaa ggttcatttg aaggttgggt aagaagaata atggtgaata
     2221 gtgcattaaa atgtattcaa aaaaagaaca aaaccaaaaa caaaacaaaa aacataattt
     2281 t
//
LOCUS       DAOQHP010000359         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_35517_length_2263_cov_3.975996, whole genome shotgun sequence.
ACCESSION   DAOQHP010000359 DAOQHP010000000
VERSION     DAOQHP010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35517_length_2263_cov_3.975996"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLY81_09755"
     CDS             complement(<1..697)
                     /locus_tag="PLY81_09755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF30552.1"
                     /translation="MKKIKYYYNTHTLRFEKIEVPLHVKLLRVFGFVAASLVSASVIF
                     FIAGRLIESPEAKLLRIQNDDWKDNYAVLQERTRQLEMQMQELEDRDNSVYRSIFESQ
                     PIPDSARVKDNEVKQEVKLIQKMGETDLVKSMSAQLNNLSLRIAYQSKSFNEIDDMVK
                     NKEKLLAAIPAIQPVSNKRLKRIASGFGNRIDPVYKVTKFHAGLDFTAPIGTPIYATA
                     DGTIKTANFNAGGY"
     gene            884..1708
                     /locus_tag="PLY81_09760"
     CDS             884..1708
                     /locus_tag="PLY81_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF30553.1"
                     /translation="MISLIKTEWLKLKKYRIFWWMLVIVALTYPGINLMFYKVYSSIT
                     AKEGMEGSLMKMLIGNPFAFPEAWHTIAYFSSWFVLVPSILIIMIISNEYTYKTNRQN
                     VIDGWSRKEFILSKLFIVLIVSIIATLMFAIITIILGLSYSTEIQATRWNEQIKYVFL
                     FLLQTFSQLSLAFFVGFIFKRSFIALGVFFFYLIIVEPSLYFLIDKYFELPTLAKLLP
                     LELSDRLIPPAAFLGKFNKEAYEQSLDNINLHILYTVIFTFLIWLFSIKIYNKRDL"
     gene            complement(1721..>2263)
                     /locus_tag="PLY81_09765"
     CDS             complement(1721..>2263)
                     /locus_tag="PLY81_09765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF30554.1"
                     /translation="VNRAGDDAVIRHILRIPPITDEEINLAKQKLDTIRKNIKEGKLT
                     FSEAVNKYSDDEESKFNGGAKIGADGSTFLSIDALDKEMVIAIKDLKEGDLSNPIVYE
                     DRGLKKVRIVFLKSRTEPHIENLREDYNKVAARALEEKKQHILEQWFKDHLPLYYITI
                     DKEFTNCADLLDWWKFAKSR"
BASE COUNT          689 a          363 c          283 g          928 t
ORIGIN      
        1 catatcctcc agcattaaaa ttggcagttt ttatagtacc atctgctgtt gcataaatag
       61 gtgtgcctat tggtgcagta aaatctaacc cagcatgaaa ttttgttact ttatatacag
      121 ggtcaatcct gtttccaaaa ccactggcaa tacgttttaa ccttttatta cttacaggtt
      181 gtatagctgg tatggcagcc aataattttt ctttattttt taccatatca tctatttcat
      241 taaaactttt agattgatat gctatcctta aggataaatt attgagttgt gccgacatgc
      301 tttttaccaa atccgtttcg cccatttttt gaataagttt tacttcttgc tttacttcat
      361 tatcttttac tcttgcacta tccggtattg gttgcgattc aaaaatactt ctataaacag
      421 aattgtctct atcttctaat tcttgcattt gcatttccaa ttgccttgtt ctttcctgca
      481 aaacagcata attatctttc caatcatcgt tttgtattct taataattta gcttctgggc
      541 tttcaattaa tcttcctgca ataaaaaaaa taactgaagc agataccaaa ctagctgcaa
      601 caaaaccaaa taccctcagc aattttacat gtagtggaac ttcaattttt tcaaacctaa
      661 gtgtgtgggt attgtaataa tattttattt tcttcattgc caagggctaa aattagtatt
      721 cttattttgt tttaaaaagt tcatttcaca cttaatcaca ttttctctct tgcaaataac
      781 aattatttaa ttaccattta aaatgtattt agtattgatt gcaatttatt tttctatttc
      841 atttatattc gtttatcatt cattaaagca gtaaatgtat tatatgattt cattaataaa
      901 aacggaatgg ttaaaactaa aaaaatatag aattttttgg tggatgctgg taattgtagc
      961 tttaacctat cctggtatta atttaatgtt ttataaagtt tactcttcta taactgccaa
     1021 agaaggtatg gagggaagtt taatgaaaat gcttattggt aatccctttg cctttcctga
     1081 agcatggcat acaattgctt atttttcatc ttggtttgta ttggtgcctt ccattttaat
     1141 catcatgatt atctctaatg aatatactta taaaacaaac aggcaaaatg ttattgacgg
     1201 ctggagtaga aaagaattta ttttaagtaa attatttatt gttttaatag tatcaataat
     1261 tgctacttta atgtttgcta ttattactat tattttagga ttaagttact caaccgaaat
     1321 acaagcaact agatggaacg agcaaattaa atacgtattt ttatttttac tacaaacctt
     1381 ttcgcaactt tctttggcat tttttgttgg ttttattttt aaaagatctt ttattgcttt
     1441 aggtgtgttt tttttctatt taataattgt agagcccagc ttatattttt taattgataa
     1501 gtattttgaa ttacctacac ttgcaaaatt attgccttta gaacttagcg atagactaat
     1561 accacctgct gcatttttag gaaaatttaa taaagaagct tatgagcaaa gtcttgacaa
     1621 tattaactta cacattttat atactgtcat tttcaccttt cttatttggc tatttagtat
     1681 aaaaatttat aacaaaagag atttataaaa gaggtataaa ttatctgctt tttgcaaact
     1741 tccaccaatc taacaaatcg gcacagttag taaattcttt atctattgta atatagtata
     1801 atggtaaatg atctttaaac cattgctcta agatatgttg ctttttttct tctaatgctc
     1861 ttgcagctac tttattataa tcttctctaa ggttttctat atgtggttca gttctgcttt
     1921 ttaaaaatac aattctcacc ttttttaaac ccctatcttc atatacaatt gggttagata
     1981 gatctccttc ttttaaatct ttaatagcta taaccatttc tttatctaat gcatctatag
     2041 ataaaaaggt tgagccatct gcaccaattt tagctcctcc attaaactta ctttcttcat
     2101 catcgctata tttatttaca gcttcactaa aagttagttt accttcttta atattttttc
     2161 taatagtatc taatttttgt ttggctaagt taatttcttc atcagtaata ggtggtattc
     2221 ttagtatatg tctaataaca gcatcatcac ctgctctgtt tac
//
LOCUS       DAOQHP010000360         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_35602_length_2260_cov_3.190930, whole genome shotgun sequence.
ACCESSION   DAOQHP010000360 DAOQHP010000000
VERSION     DAOQHP010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35602_length_2260_cov_3.190930"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLY81_09770"
     CDS             complement(<1..348)
                     /locus_tag="PLY81_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30555.1"
                     /translation="MKKILAFTVAATMLFTACNKFEKAPSGMPYKVEKGPRKQKLAHG
                     QLLKMHFEYTLKDSVISSTMGHIPVYFSLDTTRSNKYSFSEVMTDCYVGDKLFCTLSI
                     DSMVKFGYVEYNPI"
     gene            complement(345..1352)
                     /locus_tag="PLY81_09775"
     CDS             complement(345..1352)
                     /locus_tag="PLY81_09775"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNF30556.1"
                     /translation="MQPIHLFYPQLNNPSKIIIVSHQKPDGDAMGSSLGLYHFLIQLG
                     HTVNVISPTNWANFLNWMPGVEHVIDFEKNQEKCNQLIQSANIIFCLDFNVLYRTKHV
                     APLIEAVNCIKILIDHHQEPQVDAFTYGISNTAKSSTCEMVYDFIVDSGNASTLNINI
                     ATCLYTGTMTDTGSFRFPSTTASVHKMVAHFKEIGLEHAIIHDNIYDNFLENRLRFIG
                     FVLCNRLEVLYEYNTALMYVYKQDLYKYDIKTGDTEGLVNYLLTIEGIKFGAIVIDRE
                     EERKWSFRSKGNFDVNTFARLHFEGGGHKNASGGRTDDNVEDTVKRFKQTIKQYEQEL
                     Q"
     gene            complement(1416..>2260)
                     /locus_tag="PLY81_09780"
     CDS             complement(1416..>2260)
                     /locus_tag="PLY81_09780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNF30557.1"
                     /translation="IDKFNDYFVIQTLSYGMEVWKPAITKALQQIFNPKGIYERNDVP
                     VRELEGLTQQKGFLSEPFNTNIIINENGLKFNVDIENGQKTGYFLDQQDNRRAIQHIV
                     KNADVLGAFTYTGTFEIHAAHYGAKSVLGIDISENAVAQANKNAALNKLEHIVHFKAM
                     NAFDVLKVWAKEGKQYDVVMLDPPAFTKSRATLQKAITGYKEINLRGMKLVKNGGFLV
                     TSSCTNLVQPELFLHTIQLAAKDAGKKLKQLVFNAQASDHPIVWGLENTNYLKFLIVQ
                     VCDI"
BASE COUNT          737 a          383 c          311 g          829 t
ORIGIN      
        1 aattgggtta tactctacat aaccaaattt taccatacta tcaatactaa gtgtacaaaa
       61 taatttatct ccaacatagc aatctgtcat tacttcggaa aagctgtatt tattgctacg
      121 agtagtatct aaagaaaaat aaacaggaat atgacccatt gtactgctta ttacactatc
      181 ttttaaagtg tattcaaaat gcatttttaa aagctgacca tgagccaatt tttgttttct
      241 tggtcccttt tcaactttat aaggcatgcc agatggggct ttctcaaact tgttacaagc
      301 tgtaaacaac atggttgcag ctactgtaaa tgctaaaatt tttttcattg taattcttgt
      361 tcgtattgtt ttattgtttg tttaaatcgt tttacggtat cctcaacatt atcatctgtt
      421 ctaccacccg aagcattttt gtgtccacca ccttcaaaat gcaatcttgc aaaagtgttc
      481 acatcaaaat ttcctttact tctaaaactc cattttcttt cttcttctct atctataaca
      541 attgctccaa atttaattcc ttcaatggtt aataaataat tcactaagcc ttctgtatct
      601 cctgttttaa tatcatattt atataaatct tgcttataca catacattaa ggcagtgtta
      661 tactcatata atacttctaa tctgttgcat aaaacaaaac caatgaatcg caatctgttt
      721 tctaaaaagt tatcgtaaat attatcgtga atgatagcat gctctaatcc aatttcttta
      781 aaatgtgcaa ccattttatg taccgatgca gtggtagaag gaaacctgaa ggaaccagta
      841 tctgtcatag tgcctgtata taaacaagta gcaatattta tatttaaggt agaagcattg
      901 ccgctatcta ctataaaatc atataccatt tcgcaggtgc tgcttttggc tgtattgcta
      961 atgccataag taaaagcatc gacttgtggc tcttgatgat ggtctattaa aattttaata
     1021 caattaacag cttcaatcaa tggagctaca tgtttagtgc gatacagtac attaaaatct
     1081 aaacaaaaaa taatgttagc agattggatt aactggttac atttttcttg atttttttca
     1141 aaatcaatca catgttctac acctggcatc cagtttaaaa agttagccca attggtgggt
     1201 gaaattacat ttacagtatg cccaagttga attaagaaat gatacaaacc taaagagcta
     1261 cccattgcat caccatctgg tttttgatga cttactataa taatttttga aggattattt
     1321 agttgagggt aaaatagatg aattggttgc atcataaatg gttgcaaatg taatggaatt
     1381 aactattata caaataactg cataaattac atttcttaaa tatcacaaac ctgaactatt
     1441 aaaaatttta aataattggt attttctaaa ccccaaacaa ttgggtgatc ggaggcttga
     1501 gcattaaaaa ccaattgttt taattttttt cctgcatctt ttgcagctaa ttgaatggta
     1561 tgtaaaaata attcgggttg tactagattg gtgcagctac tagttactaa aaaaccacca
     1621 ttttttacaa gtttcatacc tcttaaatta atttctttat aaccagtaat agctttttgt
     1681 aaagtagctc tgctttttgt aaatgctgga gggtctagca ttacaacatc atactgtttg
     1741 ccttctttag cccatacttt taatacatca aatgcattca tggctttaaa atgtacaata
     1801 tgctctaatt tgtttaaagc agcattttta ttggcttgtg caacagcatt ttcgctaata
     1861 tcaattccta aaacagattt tgcaccataa tgggctgcat gaatttcaaa tgttcctgta
     1921 taagtaaaag cccctaatac atcggcattt tttacaatat gctgtatggc tcttctatta
     1981 tcttgttgat ctaaaaaata gcctgttttt tgaccatttt caatatctac attaaatttt
     2041 aaaccatttt catttataat aatattggta ttaaaaggtt cggataaaaa gcctttttgt
     2101 tgtgttaaac cttctaattc tcttactggt acatcgtttc tttcataaat acctttagga
     2161 ttaaaaattt gttgaagagc ttttgtaatt gcaggtttcc aaacttccat tccataagat
     2221 agtgtttgaa taacaaaata atcattaaac ttatcaatga
//
LOCUS       DAOQHP010000361         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_35673_length_2257_cov_4.747502, whole genome shotgun sequence.
ACCESSION   DAOQHP010000361 DAOQHP010000000
VERSION     DAOQHP010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35673_length_2257_cov_4.747502"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="PLY81_09785"
     CDS             complement(<1..2001)
                     /locus_tag="PLY81_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF30558.1"
                     /translation="MSICMMAGIATAQSGRTVTGVVKDVNGKPVADASVVVKGTTIGT
                     TTDSKGMFTLTSVPASAKILVVSSINFETTDVSIGNGVVSVTLKASEDDLGEVVVTVP
                     YGTVKRTAFTGSSNTVSASTINKQQVTSVSRVLEGLIPGLQATNGGGAPGSGAAVRLR
                     GIGSVNASSSPLYVVNGVPYDGSISAIASEDIESIDVLKDAAAANLYGSRAANGVIMI
                     TTKRGKGAPSLQGSIRHSFSSRGIPEYDRVGAADYYELFWEAYRNSYVYGSGQTMAQA
                     GVNASNILTGSSGLVYNAYNVPGNTLVDPVTGKLNPNAQLLWNESWSDALFRTAQRTN
                     ANVSISGSSDKANYYISGGYLNEEGAAKFSGYKRYTFRASVDIKATKYLKAGINIDGA
                     SDRRDGLFAGGTATSNPFYYSRQMGPIYPVYERNLTTGAIVKDANGNPVLDFGTTAQM
                     GSRPYAANSNLVGTLALDKRQQKRLNGNFNPYIEISFLKDFTFRTQMGLNYYAIDALT
                     YQNNQYGDAANVQGRSTVSNDYQFSLTGNQVIKWAKNFKKHNVSALAGHENYKYKSRS
                     MSATKTGFSYPGQTELDNGTVVESPPSSSEDLQTMESYFGGVNYSYDDKYLASASIRT
                     DGSSRFASDVRWGSFYSFGLGWRISQESFLKNVNWIDEMKIKL"
BASE COUNT          745 a          428 c          342 g          742 t
ORIGIN      
        1 taatttaatt ttcatttcat caatccagtt tacatttttc aagaaagatt cttgagaaat
       61 tctccaacct aaaccaaatg aatagaatga accccatcta acatcagaag caaatcttga
      121 agaaccatct gttcttatac ttgcagaagc taaatattta tcatcgtaag aatagtttac
      181 accaccaaaa taactttcca ttgtttgtaa atcttcgcta gaagatggtg gagattcaac
      241 aactgtaccg ttatctaact cagtttgacc tgggtaagag aaaccagttt ttgttgcaga
      301 catacttctt gatttgtatt tgtaattttc atgaccagct aaagcactta cattgtgttt
      361 tttaaagttt ttagcccatt taattacttg gttaccagtt aaagaaaact gataatcgtt
      421 agatacagtt gatctacctt gtacgtttgc agcatctcca tattggttat tttgatatgt
      481 taatgcatct attgcatagt agtttaagcc catttgagtt ctaaaagtga aatcttttaa
      541 gaaactaatt tctatgtatg ggttaaagtt tccatttaat cttttttgtt ggcgtttatc
      601 caaagccaaa gttccaacta agttagagtt ggcagcataa ggacgagaac ccatttgagc
      661 agttgtacca aagtctaaaa ctgggttgcc attagcatct ttaacaatag cacctgtagt
      721 taagtttctt tcatatacag ggtaaattgg acccatttga cggctgtaat agaaaggatt
      781 tgatgttgca gtaccaccag caaacaaacc atctcttcta tcagaagcac catctatatt
      841 aataccagct tttaagtatt ttgttgcttt aatatctact gaagctctaa aagtatatct
      901 tttatagcca gagaattttg cagctccttc ttcattcaag taaccaccac taatatagta
      961 gttagcttta tcagaagaac cagaaatgct aacgttagca tttgttcttt gagctgttct
     1021 aaataaagca tcagaccatg actcgttcca aagtaattga gcattaggat ttaatttacc
     1081 agtaactggg tcaactaaag tattaccagg tacattatat gcgttataaa ctaagccaga
     1141 agaaccagtt aaaatgtttg aagcatttac accagcttga gccatagttt gaccacttcc
     1201 ataaacataa gagtttctgt aagcttccca aaataattca taataatctg cagcacctac
     1261 tctgtcatat tcaggaatac ctctgctaga gaagctatgt ctgatacttc cttgtaaact
     1321 tggagcacct ttacctcttt tagttgtaat cataattaca ccattagcag ctctagagcc
     1381 atacaagttt gcagcagcag catcttttaa aacatcaata gattcaatat cttctgaagc
     1441 aattgcagaa attgaaccat cataaggcac cccatttact acatataatg gagaagatga
     1501 agcattaaca gaaccaatac ctcttaaacg aacagcagca ccagaacctg gagcaccacc
     1561 accattggta gcttgtaaac caggaattaa gccttctagt actcttgata ctgaagttac
     1621 ctgttgtttg ttaatggtag aggcagatac tgtgtttgaa gaacctgtaa atgcagttct
     1681 ttttacagta ccataaggta cagttacaac aacttcacct aaatcatctt cagatgcttt
     1741 taaagtaact gaaacaaccc cattaccaat tgacacgtca gtagtctcga aattaataga
     1801 agaaactact aaaatttttg cagaagcagg cactgaagta agtgtaaaca tacctttact
     1861 atcagttgtt gttccaatgg tagtaccttt aactactacc gatgcatcag ctacgggttt
     1921 gccgtttaca tcttttacga cacccgttac agttctgcct gactgtgctg ttgcaatacc
     1981 tgccatcatg cagatactca taagcagaaa agcgagtttt ttcataaatt gtttcatttt
     2041 tttaaacgtt taataaacca tcgtcttgcg acaaatattt aacatttatt ttaaataaca
     2101 aagccttttg taaaatattt ccttaatacg atgatttatg ttaccaaatt tagataacaa
     2161 gtctttaaca ttactaataa gacaagcttt ctattatttt gctgcaattt ggtgaaaata
     2221 aataaatcta taatcaatat attcatttat aacatat
//
LOCUS       DAOQHP010000362         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_36156_length_2241_cov_3.236048, whole genome shotgun sequence.
ACCESSION   DAOQHP010000362 DAOQHP010000000
VERSION     DAOQHP010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36156_length_2241_cov_3.236048"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1757)
                     /locus_tag="PLY81_09790"
     CDS             complement(186..1757)
                     /locus_tag="PLY81_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNF30559.1"
                     /translation="MPIRMVDDDKDNFSNENQNTPGGGGGNRGGGGLMQFLPMIIGLL
                     IRKPALLLVVLAGAAFFYFKGGCNFSSVSNIVQQFSTGGNLNKDTFAKAEVYEGLDES
                     KNNLPEYISLLKFSPNRLNQGQQGSCVAWSSAYAARTIVESTSKGIDPNAAAFSPSFM
                     YNQIGLEGCQGSYIIRAMQNLTQVGAVPFNDFPYTDKDCTNQPNGQLINEAGQNKIIG
                     FTRLTETESAAQIDIYAIKQHLAKDVPVVIGMMVGGSFMQDMMGKEIWHPTADDYNMM
                     GFGGHAMCVIGYDDRKEGGAFQIMNSWGNEWGENGIGWVLYKDFKHFVREAYGLNPMI
                     KQGAAIAKNLEVDLGLVNAETKQYIPLKTVNNNVFETVNPIPKGTGFKIELKNNTECY
                     VYVIGMETDGSSYVLFPYPSSSDATKSMFSPYCGITGYRLFPKGKKLQADDIGNKDYF
                     AIITSKEALDVFQLNNIISQNKGLGFENAINNALQKYTINGVSYNANTNGTIGFKTET
                     NNKNAVACIIAIDKY"
     gene            complement(1849..>2241)
                     /locus_tag="PLY81_09795"
     CDS             complement(1849..>2241)
                     /locus_tag="PLY81_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF30560.1"
                     /translation="QIDALEIMGINTKNYLILPKISAALLVIPCLIVLSAVLAIWGGK
                     IAGVASGIISEDVFDIGLRQNFTPYNVFFALSKAYTFAFIVSSVPAYYGYHVKGGALE
                     IGRTSTTAVVVSCILILLADYVLAALML"
BASE COUNT          744 a          406 c          303 g          788 t
ORIGIN      
        1 ataagactga aattttaatt tctattttct ttaatttaat aaagagtgtg tactagagtt
       61 ttgaaaaatg actgcttaac atttgtaaaa attacttaaa gtaatcttta ttgtttacat
      121 tttaaattag tactcaaact tatcttcatt gattaaatta gtaaataagg atgaatattt
      181 tttaattaat atttgtcaat agcaataatg catgctacag catttttgtt attggtttcg
      241 gttttaaaac ctattgttcc gttggtattt gcattgtaac taacaccatt tattgtatat
      301 ttttgcagtg cattattaat agcgttttca aagcctaacc ctttgttttg agaaatgata
      361 ttatttagct gaaaaacatc taatgcctct ttactagtaa taattgcaaa ataatcttta
      421 ttgccaatat catcggcttg taattttttg ccttttggaa ataaacgata accagtaatg
      481 ccacaataag gagagaacat tgattttgtt gcatcagaag aggatggata aggaaataaa
      541 acgtagctac ttccatctgt ttccattcca attacatata cataacactc ggtattattt
      601 tttaattcta ttttaaaacc tgttccttta ggaattggat taacagtttc aaaaacattg
      661 ttgtttactg tttttaaagg tatgtattgt ttagtttctg cattaaccaa gcctaaatct
      721 acttctaaat tttttgcaat tgcggcacct tgtttaatca ttgggtttaa gccatacgct
      781 tctctaacga aatgtttaaa atctttatac aatacccagc caattccatt ttcgccccac
      841 tcattacccc aactattcat tatttgaaat gcaccacctt cttttctatc atcgtatcca
      901 ataacacaca ttgcatgtcc tccaaagccc atcatattat agtcatcggc agtaggatgc
      961 caaatttctt tacccatcat atcttgcata aaacttcctc ctaccatcat tcctattaca
     1021 acaggaacat ctttagcaag gtgttgtttg atggcataga tatcaatttg agcagcactt
     1081 tctgtttctg ttaatctggt aaaaccaatt attttatttt gtcctgcttc gtttattaac
     1141 tgtccatttg gttggttggt acaatcttta tctgtataag gaaaatcgtt gaatggaact
     1201 gctcctactt gagttaaatt ttgcattgca cgaataatgt aactaccctg gcatccttct
     1261 aagccaattt ggttgtacat aaaagatggg ctaaaagcag ctgcattagg atcaatacct
     1321 ttacttgtag attctacaat tgttctggcc gcataagcac tactccaagc aacacaactt
     1381 ccttgttgcc cttggtttaa cctgtttgga gaaaatttta gcaatgaaat atattcgggc
     1441 aaattatttt tactttcatc taaaccttca taaacttctg ctttagcaaa agtatctttg
     1501 tttaaattac cacctgttga aaattgttgc actatgttgc taactgaact aaaattgcaa
     1561 ccacctttaa aataaaagaa agctgcacca gctaacacta ctaataataa agcaggttta
     1621 cgtataagca aaccaataat cataggtaaa aattgcatta aaccaccacc acctctgttg
     1681 cctccaccac caccaggtgt attttgattt tcgttggaaa agttatcctt atcgtcatca
     1741 accattctta taggcataat aaatatttta ggaatgaaaa taaaattaat agcaatacta
     1801 caacaagtat aataaaagtg cttgaaacta atatttttgt taataatttt agagcataag
     1861 tgcagctaaa acataatctg ctaataaaat taaaatacaa cttacaacaa cagcagtggt
     1921 acttgttcgc ccaatttcta atgcaccacc ttttacatga taaccataat aagcaggtac
     1981 gctgctaaca ataaaagcaa aagtatatgc tttactgagt gcaaaaaaaa cgttgtatgg
     2041 tgtaaagttt tgccttaacc ctatatcaaa aacatcttca gaaatgatac cacttgcaac
     2101 accagctatt ttgccacccc aaatagctaa tactgcactt aaaacaatta agcaaggtat
     2161 tactagcaat gctgcagaaa ttttaggaag aattaaataa ttttttgtgt taattcccat
     2221 tatttctaaa gcatctattt g
//
LOCUS       DAOQHP010000363         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_36415_length_2231_cov_4.053768, whole genome shotgun sequence.
ACCESSION   DAOQHP010000363 DAOQHP010000000
VERSION     DAOQHP010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36415_length_2231_cov_4.053768"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLY81_09800"
     CDS             <1..294
                     /locus_tag="PLY81_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF30561.1"
                     /translation="YDSLSETDLVEAERLYLVHCGICHGKKLDGNGPLWKDGSGPYPA
                     APKNLITLNMPDGQMFYSITYGKGQMGSYASQLSRKHRWQIVTYIKEKQKAGK"
     gene            365..1591
                     /locus_tag="PLY81_09805"
     CDS             365..1591
                     /locus_tag="PLY81_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HNF30562.1"
                     /translation="MASIKQNFEIPSKMKTWSYVLMGIGLIALIAGFITKGMSHDHHE
                     KDVFLGTLMYNTIYWTLICNTAMFFICINTMAWAGWHVSFKRVSEAISTMVPIFGAIT
                     LIVLLYIAFSGSHIYHWTDTKAVAEDPILKGKSGFLNIKFFTIWTVLAIGLWSLLGWR
                     MRKLSAQADEAAMNKETGHGFVIKNTKVAATFLVWFGLTVGSTIPWLWMMSIDAHWFS
                     TMYSWYNFASTFVSGLALLALWIIYLKNKGYLEYTNQEHLHDVAKYMFAFSIFWAYLW
                     FSQYMLIWYSNQPEEVMYFKHRVQGAYKGIFFLNLIINFICPLLILMKKASKRNYTLV
                     TFMAVLIIFGHWIDYYQQVIGSISKDHVTLGWLDFGILSLFVGMMIFGVSKALASKPL
                     IPKYHPFLKESIIHHT"
     gene            1703..>2231
                     /locus_tag="PLY81_09810"
     CDS             1703..>2231
                     /locus_tag="PLY81_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HNF30563.1"
                     /translation="MAKASEYVSILKGEEVTRKQTNKINAFLMLGFMIAGFIGVWYCN
                     EVLYHKTLLPFPAASDHGEKIDEMLWVTIWITGAVFIVTQFLLFWFAFKYQEKEGRKI
                     FYFVHSTKLEILWTSVPAIVLAVLIVVGLKHWFAITGDAPKNALEVEVVGKQFSWIFR
                     YKGKDNVFGKKYYKVI"
BASE COUNT          754 a          313 c          391 g          773 t
ORIGIN      
        1 tacgattctt tatctgaaac tgatttagtg gaagccgaaa gactttattt agtgcattgt
       61 ggtatttgcc atggaaaaaa attagatggc aatggcccac tttggaaaga tggttcagga
      121 ccttaccctg cggcacctaa aaatttaatt actttaaata tgcccgatgg acaaatgttt
      181 tacagtatta cctatggtaa aggccaaatg ggtagttatg cttctcaact aagccgtaaa
      241 caccgttggc aaattgttac ctatattaaa gaaaaacaaa aagctggtaa ataattgtaa
      301 ctattatatt taagtatttt ataaataaac agagattaag ctatactaga aaaaactaat
      361 taaaatggca tctattaaac aaaatttcga gattccttca aaaatgaaaa catggtctta
      421 tgtacttatg ggcataggat taatagcatt aattgcagga tttataacta aaggcatgag
      481 ccatgaccac cacgaaaaag atgtttttct tggtacatta atgtacaata ccatttattg
      541 gactttaatt tgcaatactg ccatgttttt tatttgtatt aatacaatgg catgggctgg
      601 ttggcacgta tcttttaaaa gagtttcaga agctatttct acaatggttc caatatttgg
      661 agctattact ttaatagttt tattatatat agcattttct ggtagccata tttaccactg
      721 gacagataca aaagcagttg ccgaagatcc tatattgaaa ggtaaatcag gcttcttaaa
      781 cataaaattc tttaccattt ggacagtttt agcaattggc ttatggagcc ttttaggttg
      841 gagaatgaga aaattaagtg cccaagccga tgaagctgca atgaataaag aaacaggaca
      901 tggatttgta attaaaaata caaaagtagc tgcaaccttt ttagtttggt ttggcttaac
      961 tgttggctct actattccat ggctttggat gatgagtata gatgcacatt ggtttagtac
     1021 aatgtatagt tggtataatt ttgccagcac atttgttagc ggattggctt tattagccct
     1081 ttggataatt tacttaaaaa ataaaggtta tttagaatat accaaccaag agcatttgca
     1141 tgatgtggca aaatacatgt ttgctttctc tattttttgg gcttatttat ggttctctca
     1201 gtacatgcta atatggtact ctaaccaacc agaagaagtg atgtatttta aacatagagt
     1261 tcaaggtgca tacaaaggaa tcttcttctt aaaccttatt ataaacttta tttgtccatt
     1321 attaatatta atgaaaaaag cttctaaaag aaactacact ttagtaactt ttatggctgt
     1381 tttaattatt ttcggacatt ggatagatta ttaccaacaa gtaattggta gcattagcaa
     1441 agaccatgta accttaggtt ggttagattt tggaatttta agcttatttg taggtatgat
     1501 gatatttggt gtaagcaagg cattagcaag taaaccactt attcctaaat accatccttt
     1561 cttaaaagaa agcattattc accatactta acagataagt atttaaaacc tgcattttaa
     1621 aaattaagaa aacagttata taaccatgac aatgttttta atcataacag tagttgttct
     1681 agtattctta gtcatttttc aagtggctaa agcaagcgaa tatgtatcta tactaaaagg
     1741 cgaagaagta accagaaaac aaactaataa aataaatgcc tttttaatgc ttggctttat
     1801 gattgctggc tttattggtg tttggtattg taatgaagtt ttgtaccata aaaccttatt
     1861 accttttcct gctgctagcg accatggcga aaaaattgac gaaatgcttt gggttactat
     1921 ttggattact ggagcagttt ttattgttac gcagttttta cttttttggt ttgcatttaa
     1981 atatcaagaa aaagaaggaa gaaaaatatt ttactttgta catagcacca aattagaaat
     2041 tctttggaca tccgttcctg caattgtttt agcagtttta attgttgttg gcttaaaaca
     2101 ttggtttgct attactggcg atgcacctaa aaatgcttta gaagtagaag ttgtaggcaa
     2161 acaatttagt tggattttta gatacaaagg aaaagacaat gtatttggta aaaaatatta
     2221 taaagttatt g
//
LOCUS       DAOQHP010000364         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_36575_length_2226_cov_3.212345, whole genome shotgun sequence.
ACCESSION   DAOQHP010000364 DAOQHP010000000
VERSION     DAOQHP010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36575_length_2226_cov_3.212345"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLY81_09815"
     CDS             <1..453
                     /locus_tag="PLY81_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNF30564.1"
                     /translation="DFYTKASAIKKGLLEFDNDKAVAENPDYVLFNGKKGSLLGANMI
                     EAKVGETVRLYVGNGGPNLTSSFHVIGEIFDNVYLEGGSTISHNIQTTMIPAGGAAIV
                     EFKCDYPGEYVLVDHSLSRAFNKGAIGKLKVTGKADAKVFNAKTIGSH"
     gene            477..1325
                     /locus_tag="PLY81_09820"
     CDS             477..1325
                     /locus_tag="PLY81_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNF30565.1"
                     /translation="MKINIFLIITVFTLLLYACNNQQKIDSKEIKIANSTTPNNSIPL
                     TVKMVYIPGGEYKPFYGSDSTLVKVPAFLLDERAVTNQEFLAFIKLNPAWRKSVIKRI
                     YADTSYLNNWVNDTTLPKDAKLDAPITNISWFAAKAFAKSIGKRLPTLDEWEYVAMAD
                     KTTANARSKPTYSNEILDLYLQKNRQYNAVKQSAPNYWGVYNMFDLIWEWTDDFNSVL
                     TTGDSRSGQYDDKNLFCAASATSATDIMNYAAFMRFALRGSVKANFAIANLGFRCAKD
                     TLILNK"
     gene            1331..1909
                     /locus_tag="PLY81_09825"
     CDS             1331..1909
                     /locus_tag="PLY81_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF30566.1"
                     /translation="MKYFFYCLISVVLFSACKENKKQTVQLPSDSIFYLTSQWKNQNG
                     DSLQLKALQGKTLAVVMIYTTCKAACPILVSKMKSIEAKIDKKNIKDVSLVLVSIDPT
                     TDTPEKLKEFSITNKMNDPQWVFLTSNDAATQEFANVLSMKYKKISPLDFSHSNIISV
                     FNPLGELVSQEEGTIDVDKVVNTINETVKKNK"
     gene            2041..>2226
                     /locus_tag="PLY81_09830"
     CDS             2041..>2226
                     /locus_tag="PLY81_09830"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNF30567.1"
                     /translation="MDSITNTQEELDMRVLVPIERHKKLIQLFKELPVGNYFIFINDH
                     DPIPLYYEFRSIYGDVVG"
BASE COUNT          809 a          336 c          365 g          716 t
ORIGIN      
        1 gatttttata caaaagcatc agctattaaa aaaggattat tagagtttga taatgataaa
       61 gcagttgccg aaaatccgga ttatgtttta tttaatggga aaaaaggttc tttattaggt
      121 gccaacatga ttgaggcaaa agttggcgaa actgttcgtt tatacgttgg taatggtggt
      181 ccaaacctta catcatcttt tcatgttatt ggagaaattt ttgataatgt ttatttagaa
      241 ggtggtagta ctatttctca caatattcaa actactatga tacctgcagg tggtgcagca
      301 attgtagaat ttaaatgcga ttacccagga gaatatgtgt tagttgatca ctctttatcc
      361 agagcattta ataaaggagc cattggtaaa ttaaaagtaa ctggcaaggc agatgctaaa
      421 gtatttaatg caaaaactat tggcagccat taatattttc taaaaaaata attgaattga
      481 aaattaatat ctttttaata attactgttt ttacattact actttatgca tgtaataacc
      541 agcaaaaaat agattcgaaa gaaattaaaa ttgctaattc aactacccct aacaattcaa
      601 tacccttaac tgtaaaaatg gtttatattc ccggtgggga gtataaacca ttttatggtt
      661 ctgactctac tttagttaaa gtgccagcct ttttattaga tgaaagggca gttaccaacc
      721 aggagttttt ggcatttatt aaattaaatc ctgcctggcg taaatctgtt ataaaaagaa
      781 tatatgccga tacaagctat ttaaataatt gggttaatga tacaacacta cccaaagatg
      841 ctaagcttga tgcacctatc acaaatatat cttggtttgc agcaaaagca tttgcaaaaa
      901 gtattggaaa aagattgcca acattagacg aatgggaata tgtagcaatg gctgataaaa
      961 ccacagcaaa tgcaagaagt aaaccaactt actccaatga aattttagat ttataccttc
     1021 aaaaaaacag gcaatacaat gctgtaaaac aatcagcacc taactattgg ggcgtatata
     1081 acatgtttga tttaatttgg gagtggactg atgattttaa ttctgtactt acaacaggcg
     1141 attctagaag tggccaatac gatgataaaa atttgttttg tgctgcaagt gcaaccagtg
     1201 caacagatat aatgaattat gcagccttta tgcgttttgc tttaagaggt tcggtaaaag
     1261 caaattttgc tattgctaat ttagggttta ggtgtgcaaa agacacttta attttaaata
     1321 aataattaaa atgaaatact ttttttactg cttaatttcc gttgttttat tttctgcttg
     1381 taaagaaaac aaaaaacaaa ctgttcaact tccttccgat tctattttct atttaacctc
     1441 acaatggaaa aaccaaaatg gcgattctct tcaactaaaa gccttacagg gaaaaacttt
     1501 agcagtagta atgatttata caacttgcaa ggcagcatgt ccaattttag tttctaaaat
     1561 gaaaagtata gaagcaaaaa ttgataaaaa aaatataaaa gatgtttcgc tagttcttgt
     1621 atctattgac cctacaactg acacccctga aaaattaaaa gaattttcaa taactaataa
     1681 aatgaacgac ccacaatggg tttttctaac ctccaatgat gctgccacac aagaatttgc
     1741 taatgttctt tctatgaaat ataaaaagat ttcgccgtta gatttttctc attcaaatat
     1801 tattagtgtt tttaaccctt tgggtgaatt ggtaagccag gaagaaggca ccattgacgt
     1861 ggataaggtt gtaaacacta ttaatgaaac ggtaaaaaag aataaataat tttaagggaa
     1921 atgatattaa tgtagtgtaa aaatttccca aatacacaaa ccttttggca ggaaaactat
     1981 aattaagcac acgctactcc ataattaaaa attatctttg caacgcaaaa aatttatttt
     2041 atggatagta taacaaatac tcaagaagaa ttagatatga gggtgcttgt gccaattgaa
     2101 aggcacaaaa aacttattca actttttaaa gaattaccag taggcaatta ttttattttc
     2161 atcaacgatc acgaccctat tcctttatac tacgaatttc gttctattta tggcgatgtg
     2221 gtaggc
//
LOCUS       DAOQHP010000365         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37172_length_2205_cov_4.044186, whole genome shotgun sequence.
ACCESSION   DAOQHP010000365 DAOQHP010000000
VERSION     DAOQHP010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37172_length_2205_cov_4.044186"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PLY81_09835"
     CDS             complement(<1..1102)
                     /locus_tag="PLY81_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF30568.1"
                     /translation="MKKTLPLIVTLITLSLLGLIILQASWLKNLIEVRQAHVFNKIQT
                     VTTQVATDLSYAAFAGPVIRLPRKSNKISAEFHLRLLKPPSIGDKFTPQEIYAKLRKA
                     FDDVDLKKMKFEFAIANSDGDYEMQSLNYAKEFYDTTSNKRIHNYLVPETGTEIDQVV
                     QAEQMIVIVPDFTKQVWRSLQWIIIGAIIFMLIIIAAFYVTLKSLFNQKKLSEIKSDF
                     INNMTHEFKTPLATISLAVDAIKNEKVKNDSDRFQYFSNIIKEENRRMNKHVETILQA
                     ALMEKQELKLNINRIALHPLLNNVIEAYNLQLLEKNGFIETNLNAKNDVVEADEVHLT
                     NLLSNLIDNAIKYSKDNLVIKISTSCNNKIFSI"
     gene            1277..1828
                     /locus_tag="PLY81_09840"
     CDS             1277..1828
                     /locus_tag="PLY81_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNF30569.1"
                     /translation="MIDIAPGILLISDPFLKDPNFARSVVLICDHQQEGSCGFVLNKL
                     YDRSLKDMLTDFEDINFPLFYGGPVQPQTIHFIHKRPDLIEGGLEVLDNIYWGGEFEL
                     VVDLIKTKKISKNDIRFFIGYSGWGEGQLADELQTKSWITRQATTALVFNSKTENIWK
                     DALKDLGGEYSQMVNYPIDPQLN"
     gene            1964..>2205
                     /locus_tag="PLY81_09845"
     CDS             1964..>2205
                     /locus_tag="PLY81_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF30570.1"
                     /translation="MDKILELNNLQKFFSSQKAVDDISFSIDKGSIFGLLGPNGAGKT
                     TLIRMITGIFYPDNGSIIFDGKKFDPINDVLKIGYMP"
BASE COUNT          747 a          299 c          314 g          845 t
ORIGIN      
        1 taatggaaaa aattttgttg ttacaagagg ttgatatttt tataaccaaa ttatctttag
       61 aatattttat tgcattatct attaaattag ataacaaatt agttaaatgt acttcatctg
      121 cttctacaac atcgtttttt gcatttaagt ttgtttcaat aaagccattt ttttccaata
      181 gttgtaaatt ataagcttca attacattat taagtaatgg atgtaaagca atacgattaa
      241 tatttagttt taattcttgt ttttccatta aagctgcttg tagtatggtt tctacatgct
      301 tattcattct tctattttct tctttaatta tattactaaa gtattgaaac ctatcgctat
      361 cattttttac tttttcattt ttaatagcat ctactgctaa agaaatggtg gctaatggtg
      421 ttttaaattc atgggtcatg ttgttaataa aatcactctt tatttcgctt aactttttct
      481 gattgaataa acttttaagt gtaacataaa atgctgcaat aattataagc ataaaaatta
      541 tagcaccaat aattatccat tgcaaacttc tccaaacctg ctttgtaaaa tcaggtacaa
      601 ttacaatcat ttgttcggcc tgtaccacct ggtcaatttc agttcctgtt tcaggtacta
      661 agtaattatg tattcttttg tttgaagtgg tatcataaaa ctctttagca tagtttaaag
      721 attgcatttc ataatcacca tcgctattgg caatagcaaa ctcaaatttc atttttttta
      781 aatctacatc atcaaaagct tttcttagct tagcataaat ttcttgtggt gtaaatttat
      841 cgcctatgct tggtggcttt aataacctta aatgaaattc tgcagaaatt ttattcgact
      901 ttcttggtaa tcttataact gggccagcaa aagcagcata acttaaatct gtagcaactt
      961 gtgtagttac agtttgtatt ttattaaaaa catgagcctg ccttacttca attaaatttt
     1021 ttaaccaaga tgcttgtaaa ataataagcc ctagtaatga aagtgtaata agcgttacaa
     1081 ttaaaggtaa agtttttttc atgctaatgt tgtagagtaa ttacactttt aaactaatta
     1141 agttttataa aacagtatta acaaatatag tattgcaaag caatttaagt aaaaatgcaa
     1201 cctttattta attgaatttt aacttactga taatattaaa tttttaaatt ttgcttgaat
     1261 gatttaattt tcggttatga tagatatagc accaggtatt ttattaattt ctgacccatt
     1321 tttgaaagat cctaattttg caagaagtgt tgttttaatt tgcgatcatc aacaagaagg
     1381 aagttgtggc tttgttttaa ataagctgta cgatcgttcg ttaaaagata tgcttacaga
     1441 ctttgaagat attaacttcc ctttatttta tggtggtccc gttcagccac aaaccattca
     1501 ctttattcat aaacgacctg atttaattga aggaggatta gaagttttag ataatattta
     1561 ttggggtggt gaatttgaat tagtagttga cttaattaaa acaaaaaaaa ttagcaaaaa
     1621 cgatattcgt ttttttattg gttatagtgg ctggggcgaa gggcaattag cagatgaatt
     1681 acaaacaaaa agctggataa ccagacaagc aaccacagct ttagttttta attcaaaaac
     1741 agaaaatatc tggaaagatg cactgaaaga tttaggaggt gagtacagtc aaatggttaa
     1801 ttatccaata gacccacagt tgaattaatt caatttatac tcacatttta gtaattcaac
     1861 tattaataaa tttcctttac ttcttccatt tttttcgcct tttgtcatta ataatttaat
     1921 aaactacttt caaagattta cttttgaaaa taaatatgta ttcatggata aaattttgga
     1981 attaaataac cttcaaaagt ttttttcatc acaaaaagca gttgatgata ttagctttag
     2041 tatagataaa ggaagcattt ttggtttatt aggccctaat ggtgcaggca aaaccacttt
     2101 aataagaatg ataacaggaa ttttttatcc tgataatggt tctataatat ttgatggaaa
     2161 aaagtttgac ccaattaatg atgttttgaa aattggctat atgcc
//
LOCUS       DAOQHP010000366         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37244_length_2203_cov_4.544693, whole genome shotgun sequence.
ACCESSION   DAOQHP010000366 DAOQHP010000000
VERSION     DAOQHP010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37244_length_2203_cov_4.544693"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2203
                     /locus_tag="PLY81_09850"
     CDS             <1..>2203
                     /locus_tag="PLY81_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNF30571.1"
                     /translation="FNHQYSLFHKQFAYCEINEGQNYYNEINGTLLHFLIEPIQECFT
                     SLQNKKFNHKISSTPEKFYDIEIHTLINTDGKIGGVALGFFDVTQRYKVAEEIKKSEL
                     LFKSLVVNATDAFQLTNEELKITYISDSVKNVLGYEAGELLHNNFFNLIHPDDKIIIS
                     TWLHWLIHNPGVVNSVELRIRNKNNEWIYIEITGNNLLHLAEVGAVVMNYKNIQNKKI
                     AENALTLAEQRMGLLLNNTKESFIVVNSRLRVITYNRAAQEHSPYFFTNELQSGVSIL
                     SLIKEVEVEKTIHTFEKVFEGYESETETIFVDDKNIVHIYNHSFRPLYSDDEIVGVFI
                     TSTDITQRKKAEQQLKISEERYKTIIRESYDAIVITDINDIIIETSPAIEKILGYKQE
                     ELTGRIFIDLIKSDYREVAFGLLQKIKNTPDEEESVDLMATTVDGDNIWIVVKAKNLY
                     HNKLIKGLVILIRNITKRKKVEEELSLSESRFKSLIQSGSDIISIVNKDGFVSYSSPT
                     VKTILGNDPEKNIGKNIFEYIHPDDIDWVRKQFSEMKSSGIKQLYFGPFRYRDGNDQY
                     RWLETVVTNLLDDPAVNGIVTNSRDVTERKKLTEKQNALREKLVKNNQDLQQFSFITS
                     HNLRAPVANLLGLLNLFNKENISDPFNEVLIEKFEDAANQLNNTLSDLIEILVLKSDS
                     EIPLEEINLKNFTHQILKNIESKLTETETIITTDFKTINSIKYNKVYLESIF"
BASE COUNT          875 a          287 c          344 g          697 t
ORIGIN      
        1 tttaaccatc aatacagttt atttcataag caatttgcgt actgtgaaat taatgaaggg
       61 caaaattatt acaatgaaat taatggcact ttacttcatt ttttaattga gcctattcaa
      121 gagtgtttta catctttaca aaataaaaaa tttaaccata aaatttcttc cactcctgaa
      181 aaattttatg acatagaaat acatacctta attaacactg atggtaaaat tggaggtgtt
      241 gcattgggct tttttgatgt tacacaacgg tataaagttg cagaagaaat taaaaaaagt
      301 gaactgcttt ttaaatctct tgtagtaaat gccacagatg catttcaatt aacaaatgaa
      361 gaattaaaaa ttacctatat ttccgattct gtaaaaaatg tattaggata tgaggctgga
      421 gaattattgc acaataattt ttttaaccta attcaccccg atgataaaat aattatttca
      481 acctggctgc attggctcat tcataaccct ggtgttgtaa actctgttga actaagaata
      541 agaaataaaa ataatgaatg gatttatata gaaataaccg gcaataacct attgcatttg
      601 gcagaggttg gtgctgtagt gatgaattat aaaaatattc aaaataaaaa aattgctgaa
      661 aatgcattaa cacttgcaga gcaacgaatg gggctattgt taaacaacac caaagaatct
      721 tttattgttg ttaatagcag gctaagagta attacttata acagagcagc tcaagaacat
      781 tcgccttatt tttttactaa tgaattgcag tctggagttt ctattttgag tttaataaaa
      841 gaggtggaag tggaaaaaac tatccacaca tttgaaaaag tttttgaagg atatgagtct
      901 gaaacagaaa caatttttgt agatgataaa aatatcgtac atatttataa tcacagtttt
      961 cgcccattat atagcgatga tgaaatagta ggtgttttta ttacctctac agatattaca
     1021 caaagaaaaa aagcagaaca acaacttaaa attagtgaag aaaggtataa aactattatt
     1081 agagaatctt atgatgctat tgtaattact gatattaatg atattatcat agaaacctcc
     1141 cctgctattg agaaaatttt gggctataag caagaagaat taactggtag aatttttatt
     1201 gatttaatta aaagcgatta tagagaagtt gcttttggtt tattacaaaa aataaaaaat
     1261 acgccagatg aggaagaaag tgtggacctt atggccacaa cagttgatgg agataatatt
     1321 tggatagttg ttaaagctaa aaatctttat cataataaat taattaaagg cttggtaatt
     1381 ttaataagaa atattaccaa acgtaaaaaa gttgaagaag aactatcctt atctgaaagt
     1441 aggtttaaaa gtttaattca aagtggttca gatataatat ccattgtaaa taaagatggg
     1501 tttgtatcct actctagccc aactgttaaa acaattttag ggaacgaccc tgaaaaaaat
     1561 attgggaaaa atatttttga atatatacac ccagatgata tagactgggt gagaaagcag
     1621 ttctctgaaa tgaaaagcag cggtattaaa caactatact ttggcccttt tagatataga
     1681 gatggaaacg accaatatcg ttggttagaa actgtggtaa ccaatctttt agacgaccct
     1741 gcagtaaatg gtattgttac caactcaaga gatgttactg aacgaaaaaa actaactgaa
     1801 aaacagaacg ctttaagaga aaaactagta aaaaacaacc aagatttaca acagttttca
     1861 ttcattacct ctcataattt aagagctcct gtagccaatt tattaggctt attaaattta
     1921 tttaataaag aaaatatttc tgacccattt aatgaagtgt tgattgaaaa atttgaagat
     1981 gcagctaacc agttaaataa tacattaagc gatttaattg aaatattagt attaaaatca
     2041 gatagtgaaa ttcctcttga agaaattaat ttaaaaaatt tcacccatca aatattaaaa
     2101 aatatagaaa gtaaactaac cgaaacagaa acaattataa ctacagattt taaaactatt
     2161 aactctataa aatacaataa ggtttattta gaaagtattt ttc
//
LOCUS       DAOQHP010000367         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37308_length_2201_cov_4.278658, whole genome shotgun sequence.
ACCESSION   DAOQHP010000367 DAOQHP010000000
VERSION     DAOQHP010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37308_length_2201_cov_4.278658"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLY81_09855"
     CDS             complement(<1..713)
                     /locus_tag="PLY81_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNF30572.1"
                     /translation="MEYRIEKDTMGEVKVPAHVLWGAQTQRSIENFKIAQDINKMPKE
                     IIEAFAYLKKAAALANNELGVLPNEKKEAIATVCDEILAGKLNDQFPLVVWQTGSGTQ
                     SNMNVNEVVAYKAHVNGGGSLLDEKKSIHPNDDVNKSQSSNDTFPTAMHIAAYKMLLQ
                     TTIPGIEKLRDTLAVKSKAFMQIVKIGRTHFMDATPLTLGQEFSGYTAQLNHGLKAIK
                     NTLAHLSELALGGTAVGTG"
     gene            951..1379
                     /locus_tag="PLY81_09860"
     CDS             951..1379
                     /locus_tag="PLY81_09860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF30573.1"
                     /translation="MALKIIDHNSKQYKQMVDMRYNILRKPLGLAFSLEELEREKEDI
                     LIGCFEEDKLEGCCLLTKVDKNTLRLRQMAVLSGLQGKGIGRVLMIFAENIARDKGYH
                     KIIMHARKNSVGFYSKLGYSTVGNEFIEITIPHIIMEKEL"
     gene            complement(1470..>2201)
                     /locus_tag="PLY81_09865"
     CDS             complement(1470..>2201)
                     /locus_tag="PLY81_09865"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF30574.1"
                     /translation="LNVKKGERVAGNSFSIGTEMMRIADLNSMVAIVDVGENDIPKVS
                     YGDTAIVSIDAYGSRKFYGIVFKIANPAASVTSTSSLNTEVANYKVHIRLLLDSYKDL
                     IVKGKPFPFRPNMSATADIQTQTVKDVLSVPLAAVTTRDSKDNAIESKSKTKSSEESE
                     EQDKTKTEEIEEVVFVKQADNKIKKIKVTTAIQDLNYIQITSGLKENDEIIVGPYSLL
                     SKTLKEGDLVKVVPKEQLYEAKKQD"
BASE COUNT          686 a          356 c          330 g          829 t
ORIGIN      
        1 attccagtgc ctacagcagt acctcctaat gccaattcac ttaaatgagc tagtgtattt
       61 ttaattgctt ttaacccatg atttaattga gcagtataac cactaaactc ttgccctaag
      121 gtaagaggtg ttgcatccat aaaatgggta cggccaattt ttacaatttg cataaaagct
      181 ttacttttga cagccaaagt atctcttagt ttttcaatac caggtatggt tgtttgtaac
      241 aacattttat atgctgcaat atgcattgct gtgggaaaag tgtcgttact tgattgagat
      301 ttattaacat catcatttgg gtgaatagat tttttttcgt ctaacaagct gcctcctcca
      361 tttacatgag ctttataagc aactacctca tttacattca tattgctttg tgtaccacta
      421 ccagtttgcc ataccactaa cggaaattga tcgtttaatt tacctgctaa aatttcatca
      481 cacactgttg ctatagcctc tttcttttcg tttggcaaaa cacctaattc gttattggct
      541 aaagcagctg ctttttttaa ataggcaaat gcctctataa tttctttagg cattttatta
      601 atatcttgtg ctattttaaa gttctcaata cttctttggg tttgtgcacc ccataataca
      661 tgagcaggaa ctttaacctc acccattgta tccttttcta tacgatattc cataatattt
      721 aaaatttgca gcaaaggtaa gttgtgagtg gtttaataat aaagtgtgtt tctaaaagtt
      781 tcttgttttt atgaactaat tcttaactat tttgttcttt tctattaaat aataaatgca
      841 ataatgttct ctcttttagt aaaattttta gagaaataat aatgtttaca ataaatttta
      901 taaaatggta ttaaaaagtt atttatcttg taaactccta acacctgctt atggctttaa
      961 aaataataga ccacaactct aaacaataca aacaaatggt tgatatgcgg tataatatat
     1021 tacgcaaacc attaggcttg gcattttctt tagaagaatt agaaagagaa aaagaagata
     1081 ttttaattgg ctgttttgaa gaagacaaat tagaaggctg ttgcttgctt accaaggtgg
     1141 ataaaaatac tttaagatta aggcaaatgg ccgttttaag tggcttacag gggaaaggta
     1201 ttggcagggt tttaatgatt tttgcagaaa atattgcaag agataaaggc taccataaaa
     1261 taataatgca tgcaagaaaa aactctgttg ggttttacag taaattagga tatagtactg
     1321 taggaaatga atttattgaa ataactatac ctcatattat tatggaaaaa gaattgtagt
     1381 tgttatgtat gcatcagtta cataacaact acaatttagt gctgtaaaga gtttttaaaa
     1441 atttcttcta aatttttaag ctaaagaaat tagtcttgtt ttttagcttc gtataattgt
     1501 tctttaggaa ctactttaac taaatcgcct tcttttaagg ttttgcttaa taagctgtaa
     1561 ggcccaacaa taatttcgtc attttctttt aagccgcttg taatttgtat ataattcaaa
     1621 tcttgaattg cagtggtaac ttttattttt tttattttat tatctgcttg ttttacaaac
     1681 actacttctt ctatttcttc tgtttttgtt ttatcctgct cctctgattc ttctgaagat
     1741 ttggtttttg atttgctttc aatagcattg tctttactat ctcttgtggt aacagcagcc
     1801 aatggaactg ataaaacatc tttcactgtt tgtgtttgaa tatctgctgt agcactcata
     1861 ttggggcgaa aaggaaatgg tttacctttt acgattaaat ctttataact atccaacaat
     1921 aatcttatat gtactttata atttgcaact tcagtattta aagaagatgt ggaagttaca
     1981 cttgctgcgg ggttggcaat tttaaaaaca attccataaa attttctact accataagca
     2041 tcaatgctaa caattgcagt atcgccatag ctaactttag gtatgtcgtt ttcgcctaca
     2101 tctacaatag caaccatgct attcaaatct gcaatacgca tcatttctgt accaatacta
     2161 aagctatttc ctgctacacg ttctcctttt ttaacattca a
//
LOCUS       DAOQHP010000368         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37515_length_2194_cov_3.796634, whole genome shotgun sequence.
ACCESSION   DAOQHP010000368 DAOQHP010000000
VERSION     DAOQHP010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37515_length_2194_cov_3.796634"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLY81_09870"
     CDS             <1..605
                     /locus_tag="PLY81_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF30575.1"
                     /translation="YWPSSIAVFGPTSPKQNCPQQTIIEPSTVYGISKYAGEFWCNYY
                     HQRFGVDVRSLRYPGLISYKSAPGGGTTDYAVEIYLEALTEKKYECFLQEDTYLPMMY
                     MPDAIRATIELMEAPKSNINVRTSYNLSGMSFSPKEIALSIKQHIPEFEITYKPDYRQ
                     EIANSWPQSIDDSSANKDWGWKPQYNLQSMTEDMLTNLKS"
     gene            complement(961..1464)
                     /locus_tag="PLY81_09875"
     CDS             complement(961..1464)
                     /locus_tag="PLY81_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30576.1"
                     /translation="MIKKIIISFFLLLFINIVFAQQIDHPDDPPYLKVPYVPPFTLIL
                     SNKDKFKKDDLPKKHNIIMIYLNTDCGHCKTLVKHIVDSVQMAPNSFFVLSCYNKLKD
                     IKEFEETNNLKSYEQIVIGRDEKYFIPTFYRVKITPFVAVYNKKGDLIKAFSEGFSIA
                     ELQKILE"
     gene            1557..2132
                     /gene="pth"
                     /locus_tag="PLY81_09880"
     CDS             1557..2132
                     /gene="pth"
                     /locus_tag="PLY81_09880"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNF30577.1"
                     /translation="MNKFLIVGLGNIGNEYKHTRHNIGFDVIDAFVIKHGGFFKLDKL
                     AEVAEVKWKGRIFICIKPTTYMNLSGKAFRYWMEKEKVELENTLTIVDDLALPIEKIR
                     LRAIGSDAGHNGLRDIQFMLQTDKYPKLRIGIGNQFPKGMQAEFVLSKWLPTEQPIIN
                     KKIEKSIEIIENFATIGIERTMTLVNNLSFT"
BASE COUNT          840 a          317 c          334 g          703 t
ORIGIN      
        1 tgtattggcc aagcagtata gcagtatttg gcccaacaag ccctaaacaa aattgccctc
       61 agcaaacaat tattgagcca tccactgttt atggtattag taaatatgct ggtgagtttt
      121 ggtgtaatta ttatcatcaa cgatttggag tagatgtaag aagcttgcga tacccaggat
      181 taataagtta taaaagtgca cctggtggcg gcactacaga ttatgctgta gaaatatatt
      241 tagaagcatt gacagaaaaa aaatatgaat gttttttaca agaagatacc tatttaccaa
      301 tgatgtatat gccagatgca ataagagcta ccattgaatt aatggaagcc cctaaaagca
      361 atattaatgt tagaacatct tacaatttat cgggcatgag tttttcgccc aaagaaattg
      421 cattatctat taaacaacat ataccagagt ttgaaataac atataaacca gattataggc
      481 aagaaattgc caatagttgg ccacagagca ttgatgacag ttctgctaat aaagattggg
      541 gctggaaacc acagtacaat ctgcaaagca tgactgagga tatgttgact aatttaaaaa
      601 gctaactttt gtatctgtta ccgctttggt aaagctggca gaaagaatac aaaaaagcca
      661 aaacacaaca ctaataagga ataaacccat tcaccttgtg ttaagtaatg aattcctaaa
      721 aatccaaata atcctaagaa acctaatggc cataatcttt tcatagcagg tacggttttg
      781 gtttaaaaat aaatagccta tatataaaac tttaattaag cagtttctta gcaataaatt
      841 taggataaac cattgagtat cgcaaacaaa aaatggcata cttattcaca ttttcttttt
      901 actgtgatta gcaattgatt ttgcttctta cattcataaa aataaatgcc taaaacatca
      961 ttattctaaa atcttttgca attctgcaat tgagaaccct tctgaaaatg cttttatcaa
     1021 atctcctttt ttgttataaa ctgctacaaa cggtgttatc ttaactctat aaaaagtagg
     1081 aataaaatat ttctcatctc taccaataac tatttgttca tacgatttta aattattggt
     1141 ttcttcaaat tctttaatat cttttaactt gttatagcaa gacaaaacaa aaaaagaatt
     1201 aggtgccatt tgtacactat ctacaatatg tttaaccagg gttttacaat gcccacaatc
     1261 tgtatttaaa tatatcataa taatattatg ctttttaggc aaatcatctt tcttaaattt
     1321 atctttatta gataatatta aagtaaatgg tggtacgtat ggaactttta aataaggtgg
     1381 atcatctgga tgatctattt gctgggcaaa aactatgttt ataaaaagca acagaaaaaa
     1441 tgaaataata atttttttaa tcattgttta aaaattaaaa aatataagat aaataatacc
     1501 cgttcaagct aaaacaaata tacttcaaat ttatactgta actttatttt tgctgtatga
     1561 acaaattttt aattgttggt ttaggaaata ttggaaatga atataaacat accaggcata
     1621 atattggttt tgatgtaata gatgcttttg taataaaaca tggcggattt tttaagttag
     1681 acaaattagc agaagtagca gaagtaaaat ggaaaggaag aatttttatt tgtattaaac
     1741 ccactaccta tatgaactta agtggaaaag cttttagata ttggatggaa aaagaaaagg
     1801 ttgaactaga aaatacctta acaattgtag atgatttggc cttgcctatt gaaaaaatta
     1861 gattaagagc tataggtagc gatgcaggac ataatggact aagagatata cagtttatgc
     1921 tgcaaaccga caaatatcca aaactaagaa ttggaattgg taatcagttt cctaaaggca
     1981 tgcaagcaga gtttgtttta agcaaatggc taccaacaga acagccaatt atcaataaaa
     2041 aaatagaaaa atctattgaa atcattgaaa attttgctac aattggtata gaaagaacca
     2101 tgacacttgt aaataattta tcttttacat aataaattgc ccctttttgc tgttattact
     2161 tactacaaat actaattaag caatgcacaa caaa
//
LOCUS       DAOQHP010000369         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37748_length_2186_cov_2.931018, whole genome shotgun sequence.
ACCESSION   DAOQHP010000369 DAOQHP010000000
VERSION     DAOQHP010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37748_length_2186_cov_2.931018"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLY81_09885"
     CDS             complement(<1..1192)
                     /locus_tag="PLY81_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF30578.1"
                     /translation="MQFYKNNLTNQELRSIYKALLLPRMIEEKMLVLLRQGKISKWFS
                     GIGQEAIAVGATLALDKEEWIMPLHRNLGVFTTRQMPLKQLFMQWQGNKEGYSKGRER
                     SFHFGTKEHFICGMISHLGPQLAIADGVALSYKLKKQDKVTVAFTGDGGTSEGDFHEA
                     LNVAAVWNLPVIFIIENNGYGLSTPVNEQYKCESLITKAKGYGMEGVQIDGNNILAVY
                     DTIKGVREYCIKNQKPYLIECMTFRMRGHEEASGTKYVPSNLFEEWAKKDPVVNFENY
                     LLQENILTTDIINIIKSEIKEYIESDLQEAYKATGLIPNTQEEINDVFANYSCHSTIQ
                     KVVNEKGEWVEEVGETEDIRFIDAIKQGLQQSMQKHHNLILMGQDIAEYGGAFKITEG
                     FVQEF"
     gene            complement(1345..1599)
                     /locus_tag="PLY81_09890"
     CDS             complement(1345..1599)
                     /locus_tag="PLY81_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30579.1"
                     /translation="MYIQSMHPNTTTGNSYLHIVACKLEELTIKVLDLQGMIAKKITL
                     PVQAGNQQLKINLNDLTGGNYVLNAFNGDVFVKSIPFIKL"
     gene            complement(1716..>2186)
                     /locus_tag="PLY81_09895"
     CDS             complement(1716..>2186)
                     /locus_tag="PLY81_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF30580.1"
                     /translation="KTSTSIHNSSNKLTNLIVQPPTTIAIALDFSEGDEKLIANALAQ
                     GNKESVFILIHIVESASAKYLGSDVDDDEARKDMDNLENYCNQLKSLGYLAIAQIGYR
                     NRVKEIQRIVQNTNSQLLIMGAHRHSGLKDYIFGETIDAVRHGLNIPVLIVNVK"
BASE COUNT          690 a          393 c          280 g          823 t
ORIGIN      
        1 caaactcttg cacaaagcct tcggtaatct taaaagcacc gccatattcg gcaatatctt
       61 gccccattaa aataaggttg tggtgttttt gcatactttg ttgcaaacct tgttttatgg
      121 catctataaa tcttatatct tctgtttctc caacttcttc aacccattct cctttttcat
      181 ttactacttt ttgaattgtt gaatggcaag aataatttgc aaaaacatcg tttatttctt
      241 cttgggtatt tggtattaaa ccagttgctt tataagcttc ttgtaggtct gattcaatgt
      301 actctttaat ttctgattta ataatattta taatatcggt agttaaaata ttttcttgta
      361 ataaataatt ttcaaaattt actacagggt ctttttttgc ccactcttca aataaatttg
      421 aaggaacata ttttgtacca cttgcttctt cgtgccccct cattctaaag gtcatacatt
      481 ctattaaata gggcttttga tttttaatgc aatattctct aactccttta atagtatcat
      541 aaacagctaa aatattattg ccatcaattt gcacaccctc cataccataa ccttttgctt
      601 tagtaataag actttcgcat ttgtattgtt catttaccgg tgtacttaaa ccatagccat
      661 tattttcaat aataaaaatt acaggcaaat tccaaacggc agctacattt aatgcttcat
      721 gaaaatcgcc ttcgctagtg ccaccatctc ctgtaaaagc aactgtaact ttatcttgtt
      781 tttttaattt ataagaaaga gccacaccgt cagcaatggc tagttgtggg ccaaggtgag
      841 agatcattcc acaaatgaaa tgctcttttg taccaaagtg aaaacttctt tctcttcctt
      901 tgctataacc ttctttatta ccttgccact gcataaataa ttgtttcaag ggcatttgcc
      961 tggtagtaaa tacacccaaa tttctatgta aaggcattat ccattcctct ttatccaaag
     1021 ctaaagttgc accaacagct attgcctctt gcccaatgcc actaaaccat ttacttattt
     1081 taccctgacg aagtaatacg agcatttttt cctcaatcat tcttggcaat aacaatgcct
     1141 tgtatataga tcgtaactct tgattagtta aattattttt atagaattgc attttacttc
     1201 tttaaaacgc taagataatt tttgtactac aatgttggca aattaattat tgatacatgt
     1261 gaaaaggttg cgttttttta gaataaaaaa agccctacta tataaatagt aaggactttc
     1321 acaaattaac cccctaacaa atttttatag ttttataaac ggaatacttt ttacaaaaac
     1381 atcgccatta aatgcattta aaacgtaatt acccccagtt aaatcgttta aattaatttt
     1441 tagctgctga tttccagctt gaacaggaag tgtaattttt ttagcaatca tgccttgaag
     1501 gtctaatact ttaattgtta gttcctctaa tttacaagcc acaatatgca aataactatt
     1561 gcctgttgta gtatttgggt gcatactttg aatgtacata agctatagtt tatctaataa
     1621 ataaaattat agtatgcatt agatacaaca ttataatatt atgttgctta gctttaataa
     1681 taaaattgaa agggataaat aaggaaaaat taggtttatt ttacatttac aattaataca
     1741 ggaatattta gaccatgcct aacagcatca atagtttctc caaaaatgta atcttttaaa
     1801 ccactatgcc tgtgtgcacc cattattaat agttgagaat tagtattttg aacaattctt
     1861 tgtatttctt taaccctatt tctgtatcca atttgtgcta tagctaaata gccaagagat
     1921 tttaattgat tacagtagtt ttctaaattg tccatatcct tccttgcttc atcatcatct
     1981 acatcacttc ctaaatattt tgcagatgca ctttctacaa tatgaattaa tataaataca
     2041 gattctttat ttccctgtgc taaagcatta gcaataagtt tttcatctcc ctcactaaaa
     2101 tctaaagcta tggcaattgt tgtaggaggt tgcactatta aattagttaa tttgtttgag
     2161 ctattatgaa tactagtaga tgtttt
//
LOCUS       DAOQHP010000370         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_37781_length_2185_cov_3.330516, whole genome shotgun sequence.
ACCESSION   DAOQHP010000370 DAOQHP010000000
VERSION     DAOQHP010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37781_length_2185_cov_3.330516"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLY81_09900"
     CDS             <1..307
                     /locus_tag="PLY81_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30581.1"
                     /translation="KKAFLSEYNVNILEVIYHNNNIKVEIMSVNDICIFKYSTRIMKL
                     DDITTTDTFKTYKEHKQNFLEAIARSIKIPSYLFKDLKKASSPTIFLSMCKILPPLL"
     gene            complement(226..1563)
                     /locus_tag="PLY81_09905"
     CDS             complement(226..1563)
                     /locus_tag="PLY81_09905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF30582.1"
                     /translation="MKKIFLFFSLFCYTVLLAQHSGSQKTKDDSWKVQYRAFPEKINN
                     LVHTKLVAKFDYSKSYLLGKAWITLQPHFYATDSLALDAKGMEIHKLDIIKNGKSEKL
                     KYNYDGMVLKINLDKTYKRTEKYTVYIEYTAKPNELKVEGSSAITDAKGLYFINPEGK
                     EKNKPTQVWTQGETEATSVWVPTIDRPNQKTTQEIYMIVPNKYVTLSNGKLVSQVKNT
                     DGTRTDYWKMDKPHAPYLFFMGIGDYAVVKDNYKGKELSYYVEHEYEKVAKKIFGNTP
                     EMMQYFSKILGVEYPWNKYAQIVGRDYVSGAMENTTATLHQESAQQDARQLVDENHWE
                     GTIAHELFHHWFGDYVTAESWSNLTVNESFANYSQTLWAEYKYGKDAGDEENYNDMQG
                     YLMSGSSKKDLVRYFYKDKEDMFDAVSYNKGGRILHMLRNIVGDDAFFKSLNK"
     gene            1717..>2185
                     /locus_tag="PLY81_09910"
     CDS             1717..>2185
                     /locus_tag="PLY81_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30583.1"
                     /translation="MKYFKLKTLICLVIVLLAFSDANAQKKHFIFVQSENNEPFYIMI
                     NNKNYSSTLSGYLIIPRLKNGKYFFITGFPKDKYPEQKFSYIINDKDAGFVLKQNGKS
                     GWSLFNVVDFTNIMANAPDWETDKIKNDTIQIEDIYAINTSSKPSTHSSSEVII"
BASE COUNT          716 a          362 c          285 g          822 t
ORIGIN      
        1 caaaaaagct tttttaagtg aatataacgt taatatttta gaggttattt atcataataa
       61 taatattaaa gtggaaatta tgagcgtaaa tgatatttgt atatttaaat atagcactag
      121 aataatgaaa ttagatgata ttacaacaac agatacattt aaaacttaca aagaacataa
      181 acaaaatttt ttagaagcaa ttgcaagaag tattaaaatt cctagttatt tatttaaaga
      241 tttaaaaaaa gcatcatcac caacaatatt tctaagcatg tgtaaaattc tgcccccttt
      301 attatagctt actgcatcaa acatatcttc tttatcttta taaaaatacc ttaccaagtc
      361 cttcttagag ctgccactca ttaaatatcc ctgcatgtca ttataattct cttcatcgcc
      421 tgcatctttt ccatatttat attctgccca taaagtttga ctatagtttg caaaactttc
      481 atttaccgtt aaattactcc aactttcagc tgtaacataa tcgccaaacc aatgatgaaa
      541 aagttcatga gcaatggttc cttcccaatg attttcatca acaagttgcc ttgcatcttg
      601 ctgtgcagac tcttggtgta gtgtagctgt ggtattttcc atagcaccac ttacataatc
      661 tctaccaaca atttgtgcat acttattcca agggtattct acacccaata ttttcgaaaa
      721 atattgcatc atttctggtg tgttgccaaa tatttttttg gctacttttt catattcgtg
      781 ttctacataa taactcaatt ctttgccctt ataattatct tttactactg cataatctcc
      841 aatacccata aaaaataaat agggtgcatg tggtttatcc attttccaat aatctgttct
      901 tgtgccgtcc gtatttttaa cttggctaac taatttacca ttacttaatg ttacatattt
      961 atttgggaca atcatgtaaa tctcttgagt agttttttgg tttggcctat caattgtagg
     1021 cacccaaaca gatgtagctt ctgtttcacc ttgtgtccat acctgtgtgg gtttattctt
     1081 ctctttaccc tctgggttta taaaatataa tcctttagca tcagttatag cagaactgcc
     1141 ttccactttc agttcattgg gttttgcagt atattcaata taaacagtat atttttctgt
     1201 tcttttatag gttttatcta aatttatttt caataccatt ccatcataat tatatttcaa
     1261 cttttcactt ttgccatttt taataatgtc taatttatgt atttccattc cttttgcatc
     1321 taatgctaaa gaatcggtag cataaaaatg aggctgaagt gttatccaag ccttgccaag
     1381 taagtagctt ttactgtaat caaatttggc aaccaacttg gtatgcacta agttgttaat
     1441 tttttctgga aatgctctgt actgtacttt ccagctatca tctttggttt tttggcttcc
     1501 agagtgttgt gccaataaaa cagtgtaaca aaataagcta aagaatagaa aaattttttt
     1561 catgaattgt gttttgtgta taaagataaa attgttgcaa agtaacggat tggttatttt
     1621 acgaaaggtt aaatattaat catttaatta tcaaattata tgtttatcgg tatatccata
     1681 tttttacata aattcgtcta tcgtttacca atttcaatga aatactttaa acttaaaact
     1741 ttaatttgtt tagttattgt tctattagcc ttttcggatg ctaatgcaca aaaaaaacat
     1801 tttatttttg tacagtcaga gaataatgag cctttttata ttatgataaa taataaaaat
     1861 tatagctcta ctttatctgg ttaccttata attccaaggc taaaaaatgg taaatatttt
     1921 tttattacag gctttccaaa agacaaatat cctgagcaaa aattctccta tataatcaat
     1981 gataaagatg caggctttgt tttaaagcaa aatggcaaaa gtggctggag cctttttaat
     2041 gtagtagatt ttacaaatat tatggcaaat gccccagatt gggaaactga taaaattaaa
     2101 aatgatacaa tacaaataga ggatatatat gctataaata cttcttcaaa accttccacc
     2161 cacagttcat cagaagtcat aataa
//
LOCUS       DAOQHP010000371         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_38515_length_2161_cov_3.221273, whole genome shotgun sequence.
ACCESSION   DAOQHP010000371 DAOQHP010000000
VERSION     DAOQHP010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38515_length_2161_cov_3.221273"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..688
                     /locus_tag="PLY81_09915"
     CDS             164..688
                     /locus_tag="PLY81_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30584.1"
                     /translation="MLFEFIGTAYSCTILFLIIVYLFTKGRIKYLFKNFIAVSNTLLI
                     LYSTYLMYQFYQLVRYIYDLNIQLPKNASEKPIDISWFQIKFVLLIFLPFLFLSKQLK
                     SNIILAGIMLVLLQWDVAADMYQSIFMQSQTAGVIFYFPYLLFYKILNYLALFISVYA
                     LLWLAKKLPYQSLK"
     gene            692..1489
                     /locus_tag="PLY81_09920"
     CDS             692..1489
                     /locus_tag="PLY81_09920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30585.1"
                     /translation="MYSPIIVALKYMQYYLKANNGKGHGAHSPFVYDFIINVLNDKRN
                     FYAYENIEKLREGLLKENSILHVEDFGAGSKIGLTKQRTVSAIASSSLKPAKYAQLLF
                     RMVNYYQPKTILELGTSLGITTAYLAAANNNAFVTTMEGSKEISSKATKNFELLELKN
                     IQQIQGNFDITLPALLPELSNIDFAFLDGNHRYLPTINYFNSILPKISNQTIVILDDI
                     HWSKEMEQAWNEIKNHESVTLSIDLFFIGILFFKSEFKVKQHFTIRF"
     gene            1578..>2161
                     /locus_tag="PLY81_09925"
     CDS             1578..>2161
                     /locus_tag="PLY81_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HNF30586.1"
                     /translation="MIAGILKEPNFENRVSLLPEQVQALIKKNVQVLVEEDAGKNASA
                     FNQAYINAGAEMVSRQKALTANLVLSIHPLTNNDLSCLNPNAVVLGVYQPLFNKDLMQ
                     SWASNNISCFSIDMIPRTTRAQSMDVLSSQANIAGYRAVLLAATLLPRYFPMFMTAAG
                     SIAPAKVLILGAGVAGLQAIATAKRLGAVVEVFDT"
BASE COUNT          772 a          301 c          324 g          764 t
ORIGIN      
        1 tcaaaacccc ggcaatgcaa ccattaagtt tagaataaaa gatgagtatg aaaaaatgga
       61 tttaaattta tcgcatggaa caaaagggtt tactatgaat aatgacatgg cagaattttt
      121 attatccaat gcagatgtgg aagttaaagt aaatagtaat taaatgttgt ttgagtttat
      181 tggcacagct tattcctgca ccattctttt tttaataata gtttatttat ttactaaagg
      241 aagaataaaa tatttattta aaaattttat agcagttagt aatactttac tcattttgta
      301 tagcacctat ttaatgtatc aattttacca actagttagg tacatttacg acttaaatat
      361 tcaattaccc aaaaatgcta gcgaaaaacc aatagatatt tcttggttcc aaataaagtt
      421 tgttttgctg atatttttac catttttgtt tttatctaaa caacttaaat caaatattat
      481 tttggctggt attatgcttg ttttgttaca atgggatgtt gcagcagata tgtatcaatc
      541 tatttttatg caatcacaaa cagcaggtgt aattttttat tttccttact tgttatttta
      601 taaaatatta aactatttag cactattcat tagtgtttat gctttacttt ggctcgctaa
      661 aaaattacct tatcaatcat taaaataaaa tatgtattct ccaatcattg ttgctttaaa
      721 atatatgcag tattacttaa aagctaataa tggtaaaggg catggtgcac attcgccttt
      781 tgtatatgat tttattataa atgtattaaa cgataaaaga aatttttatg cctatgagaa
      841 tattgaaaaa ctaagggaag ggttattaaa agaaaacagt attttacatg tagaagattt
      901 tggggctggt tctaaaattg gtttaaccaa acaacgaact gtaagtgcca ttgcttcatc
      961 ttctttaaaa cctgcaaaat atgctcagtt gctttttaga atggttaact actatcagcc
     1021 caaaactatt ctagagttag gcacttcatt aggcattaca acagcatatt tagctgctgc
     1081 aaataacaat gcttttgtta ccactatgga aggttctaaa gaaatttctt caaaggctac
     1141 aaaaaatttt gaattattag aattgaaaaa tattcaacaa atacaaggca attttgatat
     1201 aactttacct gcactgttgc cagaattatc aaatatagat tttgcttttt tagatggtaa
     1261 tcaccgatac ttgccaacca taaattattt taatagcatt ttacccaaaa tttctaatca
     1321 aacaattgta attttagatg atattcattg gagcaaagaa atggagcaag catggaatga
     1381 aataaaaaat catgaatctg ttacattaag tatagattta ttttttattg gaatattatt
     1441 ttttaaatca gaatttaagg taaagcaaca ttttactatt agattttaat attttttaca
     1501 ataactaaaa acaattaaat agattattat ccttttttca ttaaaattta tattttcacg
     1561 cctttaatac caaaacaatg atagcaggaa tactaaaaga acccaatttt gaaaacagag
     1621 tttctttatt acccgaacaa gttcaggcct taataaaaaa aaatgtacag gtattggtag
     1681 aagaagatgc aggaaaaaat gctagtgcct tcaatcaggc atatattaat gcaggtgcag
     1741 aaatggttag caggcaaaaa gctttaacgg ccaatttagt attaagcatt catccattaa
     1801 ccaataacga tttaagttgc ttaaacccca atgctgtagt acttggtgtt taccaacctt
     1861 tatttaataa agacttaatg caaagctggg catccaataa tatttcttgt tttagtatag
     1921 atatgatacc tagaacaaca agagctcaaa gtatggatgt attaagtagt caagctaata
     1981 ttgcagggta tagagcagtt ttacttgctg caactttatt acctcgatat tttcctatgt
     2041 ttatgacagc tgcaggtagt attgcacctg ctaaagtttt aatattaggt gcaggtgtag
     2101 caggtttaca agccatagct actgccaaac gcttaggagc tgtagtagaa gtttttgata
     2161 c
//
LOCUS       DAOQHP010000372         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_39154_length_2142_cov_3.620987, whole genome shotgun sequence.
ACCESSION   DAOQHP010000372 DAOQHP010000000
VERSION     DAOQHP010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39154_length_2142_cov_3.620987"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLY81_09930"
     CDS             complement(<1..913)
                     /locus_tag="PLY81_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF30587.1"
                     /translation="MDLQSIKNRFGIIGNSTELNHALNTAVQVSATDLTVLIVGESGV
                     GKEIFSQIIHALSIRKHNNFIAVNCGAIPEGTIDSELFGHEKGSFTGAVESRKGYFET
                     VNGGTIFLDEIGEMPIGTQARLLRVLETGEFIRVGSSKVQKTDVRVIAATNKELLEYT
                     SKGKFREDLYYRLNTVPIRVPALRNRKEDILLLFRKFVVDFAEKYKTVPIQLDEDAKN
                     ILTNYNFPGNVRELKNIAEQISVLSKTSFINSSQLNTFLPENKLNNMPVLANGFVSNG
                     EFANEREILYKLFFDMKKDVTELKKMFL"
     gene            complement(931..>2142)
                     /gene="miaB"
                     /locus_tag="PLY81_09935"
     CDS             complement(931..>2142)
                     /gene="miaB"
                     /locus_tag="PLY81_09935"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNF30588.1"
                     /translation="LILINTCSIREKAEQTIRKRLTEFRKLKHAKPGLLIGVLGCMAE
                     RIKAKLLEEEKLVDLVVGPDAYRSLPTLVEEAETGQKAINVLLSREETYADISPIRLD
                     SNGVTAFVSIMRGCNNMCSFCVVPFTRGRERSREVDSILAEAKALFEQGFKEVTLLGQ
                     NVDSYYWVNPINNEVVTFAMLLEKVALISPDLRVRFSTSHPKDITDDVLNTIAKYENI
                     CKYIHLPVQSGNTRILQLMNRTYTKEWYLAKVDRIKELIPECAISTDIITGFCTETEE
                     EHKDTLSVMKHSQYDFAYMFFYSERPGTLAKKRYQDDIPEETKKRRLAEVVELQNELS
                     AKSNLKDIGKTFKVLIENDSKKSNLHWMGRNSQNKVIVFPKENFNLQKGDYVNVKVNS
                     CTQATLIGEKV"
BASE COUNT          637 a          392 c          262 g          851 t
ORIGIN      
        1 ctaaaaacat tttctttaac tcagtaacat cttttttcat gtcgaaaaaa agcttgtaga
       61 gtatttctct ttcatttgca aattcgccgt tactaacaaa tccgtttgcc aaaactggca
      121 tattgtttaa tttattttcg ggtaagaaag tgtttagttg agaagaattt ataaaagaag
      181 ttttgcttaa aaccgaaatt tgttctgcta tattttttaa ctctctaaca ttacccggaa
      241 agttgtaatt agtaagaata tttttagcat cctcgtctaa ttgaatagga actgttttat
      301 atttttctgc aaaatcaaca acaaattttc taaatagtaa taatatatct tcctttctat
      361 ttcttaatgc aggtacacga attggcactg tatttaaacg ataatacaaa tcttctctaa
      421 actttccttt actagtatat tctaataatt ctttattggt agcagcaata acccttacat
      481 ctgttttttg aactttagaa gagccaactc taataaattc gcctgtttct aaaacacgta
      541 ataatctggc ttgagtacca attggcattt cgccaatttc atctaaaaat atagtaccac
      601 cattcactgt ttcaaaatac ccttttctgc tttctacagc tcctgtaaag cttcccttct
      661 catgcccaaa aagttcacta tcaatagttc cttcaggtat ggcaccacag tttactgcta
      721 taaaattatt atgttttctt atagataaag catgaattat ttgagaaaaa atttctttac
      781 caactccgct ttcacctaca attaaaaccg ttaaatctgt tgccgaaact tgtacagcag
      841 tatttaatgc atggtttaat tctgtagagt tgcctataat accaaaccta ttttttatac
      901 tttgtaaatc cactattttc ttttttaggg ttaaactttt tctcctatta atgtagcttg
      961 tgtgcaacta tttactttca catttacata atctcctttt tgtaagttga aattttcttt
     1021 tggaaaaaca attactttgt tttgagaatt tctacccatc cagtgtaaat tacttttctt
     1081 actatcgttt tctattaaaa ctttaaatgt tttaccaata tcttttaaat tacttttagc
     1141 agatagttca ttttgcaatt ctaccacttc tgcaagtctt ctttttttag tttcttctgg
     1201 tatatcatct tggtaccttt ttttagctaa agttccgggt ctttcgctgt aaaaaaacat
     1261 atatgcaaaa tcgtattggc tatgcttcat tacacttaat gtatctttat gttcttcttc
     1321 agtttctgta caaaagcctg taattatatc tgtactaatt gcacattctg gtattaattc
     1381 tttaattcta tccacttttg ctaaatacca ttcttttgta taagttctgt tcattaattg
     1441 taatattctg gtattgccac tttgtacagg caaatgaata tacttgcaaa tattttcgta
     1501 tttagcaatg gtgtttaaaa catcatctgt aatgtcttta gggtgagagg tgctaaacct
     1561 aactcttaaa tcagggctaa ttaaagctac tttttctaat aacatggcaa atgttaccac
     1621 ttcattattt attgggttta cccaataata gctatctaca ttttggccta ataaggtaac
     1681 ttctttaaag ccttgttcaa ataatgcttt tgcttcggct aaaatagaat ctacttctct
     1741 gcttctttct ctgcccctag taaatggtac tacacaaaag ctacacatat tattacagcc
     1801 cctcattatt gatacaaagg cagtaacacc attactatct aaacgaatag gagaaatatc
     1861 tgcataagtt tcttctctgc ttaataaaac gtttattgct ttttgtcctg tttctgcttc
     1921 ttcaactaat gtaggtaagc ttctgtaggc atctggtcct acaactaaat caactaattt
     1981 ttcttcttct aataatttgg ctttaattct ttctgccata caacctaaaa caccaattaa
     2041 taaacctggt tttgcatgtt ttaattttct aaactcagta agccttttcc taattgtttg
     2101 ttctgctttt tctctaatag agcaggtgtt aattaatatt aa
//
LOCUS       DAOQHP010000373         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_39608_length_2128_cov_2.841775, whole genome shotgun sequence.
ACCESSION   DAOQHP010000373 DAOQHP010000000
VERSION     DAOQHP010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39608_length_2128_cov_2.841775"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..549)
                     /locus_tag="PLY81_09940"
     CDS             complement(94..549)
                     /locus_tag="PLY81_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30589.1"
                     /translation="MLAKTRTIFKELVEIYTGKDKRLSQMASWSLSYAVSKNSTLLQP
                     HLKEIINQLNRKDVHDAVIRNALRILQDIEIPIKFHGTVMHTCFNFIETPSTPVAIKA
                     FSLTILYNLSKIYTEIKPELICLIETNIQHETAAFKSRGNKILKLLKNK"
     gene            complement(632..>2128)
                     /gene="uvrC"
                     /locus_tag="PLY81_09945"
     CDS             complement(632..>2128)
                     /gene="uvrC"
                     /locus_tag="PLY81_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNF30590.1"
                     /translation="VIKNEPFPRLFFTRKKINDGSEYLGPFTSLKKVKELLEFIKLYI
                     PLRNCNLLLSPTNIAKKKFKVCLEYHLGNCKGPCEDLQTVDNYNENLQQVRNILKGNI
                     TPVIQHLKVEMEEKATNLQFEKAAIIKRKIDDLETYQSKSIIVNHLLSNIDVFSIVSE
                     HEVAYVNYLMIQNGTIVQTHNIQLSIPLEQTNQQVLSYAIPQIRSNFNSTANEIIVPF
                     KVDIVHSSIKITIPKLGDKKKLLELSLKNVSYYKQELQQKKFLHLTTSSNIENNETLI
                     ELQQYLRLKDLPTHIECFDNSNFQGSYPVSAMVCFKNGVPSKNDYRKFNVKTVIGIND
                     FATMQEVVYRRYKRLIEEEKPLPQLIIIDGGKGQLSAALKSIKKLSIENKLTVIGLAK
                     NEEQLFFNEDQQPITLPLNSKSLKLIRFIRDEVHRFGITFHRNQRSKGTFKNELELIP
                     GIGKQTVTELLKTFKSVNAIKSKTHTELSEVIGNKRAGILIDYFNSKN"
BASE COUNT          662 a          297 c          289 g          880 t
ORIGIN      
        1 aaagtaaata gtatttttac gatagtttaa agcagtagtc taaaattgag ataataattc
       61 tataattggt aatacttggt tgtaagtaca atcctacttg ttttttaaga gctttaaaat
      121 tttatttcct ctacttttaa aagcagcagt ttcatgctgt atattggttt ctattaagca
      181 aattaactca ggttttatct cggtatatat tttacttaaa ttgtacaata tggttaatga
      241 aaaagctttt atagctacag gggttgatgg tgtttcaata aaattaaaac aagtatgcat
      301 tactgtacca tgaaatttta tgggtatttc aatatcttgt aaaattctta aagcatttct
      361 tataactgca tcgtgtacat cttttctatt taattggttt attatttctt ttaaatgagg
      421 ttgtagtaga gtagagtttt tgcttactgc ataacttaaa ctccatgatg ccatttgaga
      481 caatcgttta tccttacctg tataaatttc caccaattct ttaaaaatgg ttcttgtttt
      541 ggcaagcata agttgcaata gcttctatat taatttttga ataactattt tgtagcgatt
      601 tttttatatc caatgtataa gaattaagga attaattttt tgaattgaaa tagtctatta
      661 aaataccagc ccttttattt ccaataactt ctgaaagctc tgtatgggtt ttgcttttta
      721 tagcattaac cgatttaaat gtttttaata attcggttac tgtttgttta ccaattccag
      781 gtataagttc taactcgttt ttaaatgttc ctttagaacg ttggttacga tgaaaggtaa
      841 ttccaaatct gtgaacttca tctctaataa acctaataag ctttaaactt ttactgttta
      901 atggtagtgt tataggttgt tggtcttcat taaaaaataa ttgttcttcg tttttagcta
      961 aaccaataac agttaattta ttttcaatag atagtttttt tatactcttc aaggcagcac
     1021 ttaactgccc tttgccacca tctataatta ttagttgtgg taaaggtttt tcttcttcta
     1081 ttaatctttt gtaacgccta taaacaactt cttgcatagt tgcaaaatca ttaataccaa
     1141 ttactgtttt aacattaaat tttctataat cgtttttact gggtacacca tttttaaaac
     1201 aaaccattgc actaacaggg taactgcctt gaaaattaga gttatcaaaa cattcaatat
     1261 gtgttggtaa atcttttaat cttaaatatt gctgtagctc tatcaaagtt tcattatttt
     1321 ctatattgct actggttgtt aaatgcagaa acttcttttg ttgtaattct tgcttataat
     1381 aagaaacatt ctttaaagat agttctagta gttttttctt atctcctaat tttggaattg
     1441 ttatcttaat ggatgaatga acaatgtcca ccttaaaagg cacaattatt tcattggcag
     1501 tgctgttaaa attgcttctt atttgaggta ttgcataact taaaacttgc tgattagttt
     1561 gctctaaagg aatacttaat tgtatattat gcgtttgcac aattgttcca ttttgaatca
     1621 ttaagtaatt tacataagca acttcatgtt cactaacaat agaaaataca tctatattgc
     1681 ttagtaaatg gttaacgatt atagatttag attggtaagt ttctaagtca tctatttttc
     1741 gtttaattat agctgctttt tcaaattgca aattggtagc tttctcctcc atttctactt
     1801 ttaaatgctg tataacgggt gtaatatttc cctttaaaat atttctaacc tgttgtaaat
     1861 tttcattgta attatctact gtttgcaaat cctcacatgg acctttacag ttgcctaaat
     1921 gatactctaa acatacttta aatttctttt ttgcaatatt agttgggctt aataataaat
     1981 tgcagttgcg taaaggaatg tacagtttga taaattctaa taattctttt actttcttta
     2041 atgaagtaaa agggcctaaa tattctgagc catcattaat tttttttctt gtaaaaaata
     2101 atctaggaaa tggttcattt tttataac
//
LOCUS       DAOQHP010000374         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_39610_length_2128_cov_2.644477, whole genome shotgun sequence.
ACCESSION   DAOQHP010000374 DAOQHP010000000
VERSION     DAOQHP010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39610_length_2128_cov_2.644477"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            521..838
                     /gene="trxA"
                     /locus_tag="PLY81_09950"
     CDS             521..838
                     /gene="trxA"
                     /locus_tag="PLY81_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNF30591.1"
                     /translation="MALEFTDANFQQEVIESDKLSVVDFWAEWCGPCRAIGPVIEELA
                     KEYEGKVKIGKVNVDVNPNVSVNYGITSIPAILFIKGGKIVDKQIGAVPKNVLDKKIQ
                     ANM"
     gene            complement(876..1256)
                     /locus_tag="PLY81_09955"
     CDS             complement(876..1256)
                     /locus_tag="PLY81_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30592.1"
                     /translation="MRLKYLVFLIILSSFIGYLEWGTESKTFLIIAEAEIIHKIITHP
                     SSVIHPFILIPLLGQLLLVYQLIKNKLQKIILYLAISCIALLFIFMLFIAITTLNLKI
                     LLSVMPFFVVSIYTLYSIKKSPIN"
     gene            complement(1274..>2128)
                     /locus_tag="PLY81_09960"
     CDS             complement(1274..>2128)
                     /locus_tag="PLY81_09960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNF30593.1"
                     /translation="KFSDGEFQPIFLESIRGDYVFLIQSTFAPTENLMELLLMIDAAK
                     RASASRIVAVIPYYGFARQDRKDKPRVAIGSKLIATLLQAAGADRIITMDLHAPQIQG
                     FFDIPVDHLDSSAIFIPYIEQLQLGNLTFAAPDVGSTNRVREIASYFNAEMVICDKHR
                     KRANEIASMVVIGDVTNKDIVLIDDICDTGGTLAKSAALLKEKGAKSVRAFCTHPVLS
                     GKAYENIENSVLEELVVCDTIPLKQESGKIKTISVADLFAIAIRNAYENKSITSLFVH
                     SQLRGRDK"
BASE COUNT          786 a          319 c          325 g          698 t
ORIGIN      
        1 gggaaaacag aagagtctat tcctatcatt agacgagcat atctgcactt ccgcagatta
       61 ttgcctccgc ccaatatttt tacaaacttt ctgaccattt catcagtaga gtatatcatg
      121 gatggaaaga tgctgatgga tatatagcca tatctgcacc tcagatcaat aatcatgaac
      181 ttgaatgggt aatacagaat agaaatacga atattcctcc agatacttta gtcacggagt
      241 ggtttcaaat aagtaaccaa gcattattaa cacaaaaaat aaaaacacaa ggctggataa
      301 ttatttctat tgtaataatt ttagcacttt gtgttttagg ctatttggtt ataaagcaaa
      361 ttaagtaatt acaataatat gccccaatgg cagttttgag cttaaatagt cataaataac
      421 attggtggaa aagtcaaaac agcacaaaat gtaaattgga tatatatttg atgtttaaac
      481 tcaaggaaaa gttttattaa aataaattaa atttacaatt atggcattag aatttacaga
      541 tgcaaacttt cagcaagaag taatagaaag tgataaatta tcggttgttg atttttgggc
      601 agaatggtgt ggaccatgta gagcaattgg ccctgtaata gaagagttag caaaagaata
      661 tgaaggaaaa gtaaagatag gaaaagtaaa tgtggatgtt aaccccaatg ttagtgtaaa
      721 ctatggcatt acatctatac cagccatatt gtttattaaa ggtggtaaaa tagtagataa
      781 acaaattggt gctgtaccta aaaatgtatt agataaaaaa attcaggcaa acatgtaata
      841 atatttgttt aaaattttaa gccctagtaa ttagtttaat taatggggct tttttttata
      901 ctatataaag tataaatact tacaacaaaa aaaggcatta cagataaaag aatttttaaa
      961 ttaagtgttg taattgcaat aaaaagcata aatataaaaa gcaaggcaat acaacttatt
     1021 gctaaatata aaataatttt ttgtagttta tttttaatta actgataaac taaaagtaat
     1081 tgccctaata atggtattaa tataaaagga tgaataacag aggaagggtg agtaataatt
     1141 ttatgaatta tttcggcttc tgcaataatt aaaaaggttt tactttcggt accccattct
     1201 aaatatccaa taaaagaaga tagaataatt aaaaatacaa gatatttaag cctcataaaa
     1261 agagataaat tttttactta tctctgcctc ttaactggct atgaacaaat aaactggtta
     1321 tacttttatt ttcataagca ttgcgtatag ctattgcaaa caaatcggca actgaaatag
     1381 ttttaatctt accactttct tgttttaaag gaatagtatc gcatactact agttcttcta
     1441 aaacactatt ttcaatattt tcgtatgctt tgccacttaa aacaggatgt gtacaaaagg
     1501 ctctaacact tttggcacct ttttctttta atagagctgc acttttagca agtgttcctc
     1561 ctgtatcaca aatatcatct atcaacacaa tgtctttatt ggtaacatct cctataacaa
     1621 ccatactagc aatttcgttg gcacgtttac gatgtttatc gcaaataacc atttctgcat
     1681 taaaataact ggcaatttct cttactcggt tagtactacc tacatctggt gcagcaaaag
     1741 ttaagtttcc taattgtaat tgttcaatat aaggtataaa aatggcacta ctatctaaat
     1801 gatctacagg aatatcaaaa aagccttgaa tttgtggtgc gtgtaaatcc attgtaataa
     1861 ttctatctgc acctgcagct tgtagaagtg tagcaattaa tttactgcca attgcaacac
     1921 gtggtttatc ctttctatct tgccttgcaa aaccataata tggaataaca gccacaattc
     1981 tactggcact tgctctttta gcagcatcta tcattagcaa aagttccatt aaattttcgg
     2041 tgggtgcaaa tgtgctttga attaaaaaaa cataatcgcc tctaatactt tctaaaaaaa
     2101 tgggttgaaa ttcaccatca ctaaattt
//
LOCUS       DAOQHP010000375         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_40751_length_2094_cov_4.535066, whole genome shotgun sequence.
ACCESSION   DAOQHP010000375 DAOQHP010000000
VERSION     DAOQHP010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40751_length_2094_cov_4.535066"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PLY81_09965"
     CDS             complement(<1..1553)
                     /locus_tag="PLY81_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30594.1"
                     /translation="MKLVLLVFFSVLFHTLLQAQQPHSLLHKADSLATIGEYEAAIKL
                     REQHLQTINSNDTAYNDYIFSLAKLYNANKQYYKAVNLYETSLNGYLKNTEKNKEKLA
                     DNYNNLALAYEAIGRYDKSISYLTKAYNIVRKYYSNNIGSNITGMGNLITALIDNGDM
                     KAAKNLLDTLNEYRANFNHPPLRKEWNKLNAYEKWNRDIFILLTAIRYYANAGDEKNI
                     QTHIAALEKKFTPPLSTEKKNDLAHLLEAYDAIAYQYRISKNYTKALEYYAKAEALVH
                     EDFYKMKAAAHKAIIYNIMGKYQDAYSYVEKALTVFQFPPENNSHYGLIALKAELLQS
                     LHKNKEAVKQLEQLYSLLLKQNITADNFSTFFLNQFTHKINHTQIIIFIKSGNIYYGM
                     FNQSKQKEHLTTATHFYTLAAQLFKTYYSREFYNKALDDLNKQITEKILTVSLEQKNT
                     DYSSAVNLIENNTSKHLWKKFLSRYSENLKTPYELLNKRNMLAAALSNYEILDNKTQE
                     DKKQQQQLN"
     gene            complement(1569..1760)
                     /locus_tag="PLY81_09970"
     CDS             complement(1569..1760)
                     /locus_tag="PLY81_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30595.1"
                     /translation="MPNYNAKEFFYDSLKAKRIENIEYLLNITSEEWKDFEESIFPSN
                     KRRLNDSQKQAIYKPFYTE"
     gene            complement(1862..>2094)
                     /locus_tag="PLY81_09975"
     CDS             complement(1862..>2094)
                     /locus_tag="PLY81_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF30596.1"
                     /translation="KGLKGNLLLVHGTGDDNVHYQNTELLINELVKFNKQFTLMSYPN
                     RSHSISEGKGTFQHLSTLFTNYLKQHCPGGGR"
BASE COUNT          594 a          287 c          354 g          859 t
ORIGIN      
        1 tggtttagtt gttgctgttg ttttttatct tcttgggttt tattgtcaag tatttcgtaa
       61 ttgcttaatg ctgctgcaag catatttctt ttgttcagca attcgtaagg ggttttaaga
      121 ttttcgctgt aacggctcag aaattttttc cataaatgtt tggatgtgtt gttttctata
      181 agatttacgg ctgatgagta atctgtattt ttttgttcaa gagatacagt caatattttt
      241 tctgttattt gtttgttcag gtcatctaat gctttattgt aaaattctct ggagtaataa
      301 gttttaaata attgtgcagc taatgtataa aaatgtgttg ctgtggtaag atgttctttt
      361 tgtttgcttt ggttgaacat tccataataa atatttccag actttatgaa aataataatt
      421 tgtgtatggt ttattttatg tgtaaactgg ttgaggaaaa aagttgaaaa attatctgca
      481 gtaatatttt gtttaaggag taggctgtaa agttgttcta attgctttac agcttctttg
      541 tttttatgca acgattgtaa tagctctgct tttaaagcta ttaaaccata atgactgttg
      601 ttttctgggg gaaactgaaa aacagttaat gctttttcta cataactata agcatcttga
      661 tatttgccca ttatattata aataatagct ttgtgggcgg cggctttcat tttgtaaaaa
      721 tcttcatgca caagagcttc ggctttggca taatattcta atgcttttgt ataattcttg
      781 cttatgcgat attgatatgc tattgcatca taggcttcca ataaatgggc taaatcattt
      841 ttcttttctg tggataaagg cggtgtaaat tttttttcta atgctgctat atgtgtttgt
      901 atattttttt catcacctgc attggcataa tatcttatgg ctgtaagtaa aataaaaata
      961 tctctattcc atttttcata agcgttcagt ttgttccatt cttttcgtaa aggagggtga
     1021 ttgaaatttg ctctgtattc attcaatgta tctaacagat ttttggcagc tttcatatcg
     1081 ccattgtcta ttaaagcagt aataaggttt cccatacctg ttatgttact gcctatgttg
     1141 ttgctgtaat attttcttac aatattgtat gctttggtaa gatagctgat gcttttgtcg
     1201 tacctgccaa tagcttcata agcaagagca aggttgttgt agttatcagc taatttttct
     1261 ttattttttt cagtgttttt taaataccca tttaaagaag tttcataaag atttacagcc
     1321 ttataatatt gtttatttgc attgtataat ttggctaagg aaaatatata atcgttgtat
     1381 gcagtgtcat tggaattgat tgtttgtaag tgttgctctc ttagttttat agcagcttca
     1441 tattccccta ttgttgctaa actgtctgct ttgtgtagta aggaatgggg ttgctgtgct
     1501 tgcagcagtg tatgaaacaa aacggaaaaa aagacaagca atactaattt catcttgtat
     1561 aaacaggttt attctgtata aaatggtttg taaatggctt gcttttgaga atcatttagc
     1621 cttcttttat ttgatggaaa aatgctttct tcaaagtctt tccactcttc acttgttatg
     1681 tttaataaat actcaatatt ttcaattctt ttagctttta acgaatcgta gaaaaactcc
     1741 tttgcattgt agtttggcaa aggttggtta cctacttaaa aaaaatattt tttttaaaat
     1801 tacatcatta gcactcaaaa tcaattaaag caaaaaacta aaaatatcgt tagtgcaaga
     1861 actatcttcc acctcctggg caatgttgtt ttaaataatt tgtaaatagt gttgataaat
     1921 gttgaaaagt acctttgcct tcgctaatac tatgagaacg attagggtaa gacattagag
     1981 tgaactgctt attaaattta accaattcat taattaacaa ttctgtgttt tgataatgca
     2041 cattatcatc tcctgttcca tgtactaata gtaaatttcc tttcaatcct tttg
//
LOCUS       DAOQHP010000376         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_40960_length_2088_cov_4.118052, whole genome shotgun sequence.
ACCESSION   DAOQHP010000376 DAOQHP010000000
VERSION     DAOQHP010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40960_length_2088_cov_4.118052"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLY81_09980"
     CDS             complement(<1..1662)
                     /locus_tag="PLY81_09980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNF30597.1"
                     /translation="MWYKLGQFILKNRLLLLIALLITTALMGFFASKVKLSYEFTRAI
                     PTDNIKYKDYQQFRQKFGDDGNTMVFGIESNQFFTPSIFNEVANFHKNLKQIRGVTNV
                     LSIPEAVTLVKDSVGEKLLPQKIFNAPYTTQENLDIDKTLFLSLPFYNSLLYNANTNA
                     YIIGVQLNKDSVNSKSRTELINNVMAQAKTFEKNTNISLHTSGLPFIRTTIGNRIKDE
                     MNWFLIGSLVLSAITLMLFFRSLSATVMSLLVVLFGVVWSLGTIVLLGYKITLLTALI
                     PPLIVVIGIPNCIYFLNKYHSTFKETGNKNHALVTMVGRMGVVTLFCNIAAAIGFAVF
                     AFTKSNLLIEFGIVSGINIMALFIISLIFIPIVLSYLPEPKPKHVKYLDNKILEKLLV
                     KIERWSLNNAKWIYTLTIIFCTLAIIGFLKLKSEGFIVDDLPKQDKIYTDLKWFEKNM
                     KGVMPLEIMVDTKKKNGLLRNMNPINKIEELSTYIASKEEAARPMSFVEGLKFAKQAY
                     YDGDSLSYAVPYEGDMAFLGPYLKSNNSSDTVGAKKQNTTSLLNGF"
     gene            complement(1720..>2088)
                     /locus_tag="PLY81_09985"
     CDS             complement(1720..>2088)
                     /locus_tag="PLY81_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF30598.1"
                     /translation="CAIVPYKKDLWWIGSNYEWNFENDLPSNHFREDMEARLKSFLKI
                     PYKIVEHIAAIRPANIERRPFIGFHPIHQNIGILNGMGTKGCSLAPYFAHQFSNYIVQ
                     GTAIHSMADIKRFSGILSKN"
BASE COUNT          711 a          331 c          277 g          769 t
ORIGIN      
        1 aaaaccatta agcaaactag tagtattttg tttttttgca ccaaccgtat cgcttgaatt
       61 attagatttt aaataaggcc ccaaaaaagc catatcacct tcgtaaggta ctgcatagct
      121 taaactgtct ccatcataat atgcttgctt tgcaaatttt aatccctcta caaaactcat
      181 aggtcttgct gcttcttctt tacttgcaat gtaagtagat agttcctcaa ttttatttat
      241 tgggttcatg ttgcgtaata acccattttt ctttttggta tcaaccatta tttctaatgg
      301 cattacacct ttcatgtttt tttcaaacca ttttaagtcg gtataaattt tatcttgttt
      361 aggtaaatca tctactataa atccttcgct ttttaatttt aaaaaaccta taatagctaa
      421 agtgcaaaaa attattgtta aggtataaat ccactttgca ttgtttaatg accaacgttc
      481 tatttttact aatagttttt ctaaaatttt attatctaaa tatttaacat gttttggttt
      541 aggttctggc aaataactta aaacaatagg aataaaaatt aatgaaataa taaataaagc
      601 cataatgttt atgcctgata caataccaaa ctcaattaat aaattacttt ttgtaaatgc
      661 aaatactgca aaaccaatag ctgcggcaat attgcaaaaa agtgtaacaa cacccatacg
      721 cccaaccata gtaactaatg catgattttt attgcctgtt tctttaaatg tagaatggta
      781 tttgtttaaa aagtagatac aattaggaat gccaattaca acaattaaag gtggtattaa
      841 agcagttaac aatgttattt tgtaacctaa taatactatt gttcccaagc tccaaacaac
      901 accaaaaagt acaaccagta aactcattac tgtagcactt aaacttctaa aaaagagcat
      961 taaggtaata gctgataaaa ctaatgagcc tattaaaaac caattcattt catctttaat
     1021 tctattacca attgttgttc ttataaaagg caagccacta gtatgcaaac taatatttgt
     1081 atttttttcg aatgttttag cttgtgccat tacattgttt atcaactctg ttctactttt
     1141 actatttacg ctgtctttgt ttagctgaac accaattata taagcatttg tattagcatt
     1201 atataaaagt gaattataaa aaggcaagga taaaaacaag gttttatcta tatctaaatt
     1261 ttcttgagtt gtgtacgggg cattaaatat tttttggggt aataattttt ctccaaccga
     1321 atcttttact aaggttactg cttcgggtat gcttaaaaca tttgtaacac cacgtatttg
     1381 ttttaagttt ttatgaaaat ttgctacctc attaaatatt gaaggtgtga aaaattggtt
     1441 gctttcaatt ccaaaaacca ttgtgttgcc atcatctcca aatttttgtc tgaactgctg
     1501 ataatctttg tatttaatat tatctgtagg aatggctcta gtaaactcgt agcttaattt
     1561 tactttagat gcgaaaaacc ccattaaggc agttgtaata agtaaagcta ttaatagtaa
     1621 tagcctattt tttaaaataa actgcccaag tttataccac atactttttt tattataact
     1681 gttttgaaag ttgcaaagaa aagtattttt atagaagtat taatttttag ataagatgcc
     1741 agaaaatctt ttaatatctg ccatagaatg aattgctgtt ccttgtacaa tataatttga
     1801 aaattgatgg gcaaaatatg gtgctaaaga acagcctttt gtacccatgc catttaaaat
     1861 tccaatattt tgatgaatag gatgaaagcc aatgaatggc cttctttcaa tatttgcagg
     1921 ccttatagca gctatatgtt caacaatttt ataaggaatt ttaagaaaac ttttcaacct
     1981 tgcttccata tcttctctaa agtggttact gggtaaatca ttttcaaaat tccattcata
     2041 attggagcca atccaccata aatctttttt gtatggtaca attgcaca
//
LOCUS       DAOQHP010000377         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_41209_length_2081_cov_2.607108, whole genome shotgun sequence.
ACCESSION   DAOQHP010000377 DAOQHP010000000
VERSION     DAOQHP010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41209_length_2081_cov_2.607108"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /gene="eno"
                     /locus_tag="PLY81_09990"
     CDS             complement(<1..476)
                     /gene="eno"
                     /locus_tag="PLY81_09990"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF30599.1"
                     /translation="MSYIIEVHARQILDSRGNPTVEVDVLTDDGYFGRAAVPSGASTG
                     IHEAAELRDNDKKKYVGKGVLKAVKNVNDTISKALVGYDVAQQAAIDQVMIDLDGTPN
                     KSKLGANAILAVSMAVAKAAAEEAGLPLYRYVGGTNAKTLPVPMMNILNGGAHADN"
     gene            complement(530..1252)
                     /locus_tag="PLY81_09995"
     CDS             complement(530..1252)
                     /locus_tag="PLY81_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF30600.1"
                     /translation="MSIKVAGITKIYGTQKAVNNINFNINKGEIVGFLGPNGAGKSTT
                     MKILTGYLAATVGNAFINEINVQENPILTKKQIGYLPESNALYLDMYVKEYLKFIAQI
                     HQLTNSAKAVLHVIELTGLQIEQNKKIGQLSKGYKQRVGLAAALIHNPQVLILDEPTS
                     GLDPNQIIEIRNVIKEQGKNKTVLFSSHILQEVEAICDRIIVINRGEIVADDKLENLK
                     NKGKLEDIFQQLTSKNNIILQN"
     gene            complement(1263..1562)
                     /locus_tag="PLY81_10000"
     CDS             complement(1263..1562)
                     /locus_tag="PLY81_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30601.1"
                     /translation="MFYSMLLKLISQYYIYLWLLMAVVIGIKILLTFFFNKNVEGFQG
                     LLYSIFKWYNQDDMDMAEGNKRKKLMSFYNLLTVAMYFMLGLIILFSILPMFLTV"
     gene            1723..>2081
                     /locus_tag="PLY81_10005"
     CDS             1723..>2081
                     /locus_tag="PLY81_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30602.1"
                     /translation="MKILLFYIKRKYTCYIVSIFFTIFSTPLFYSTTQAQTFAELNKL
                     FISLYDNGKYDSAVLIGEKALIQCEKEYGKKDINYAISYLNIADAYAALKKISEAVLN
                     YSKSIEVFAIVYNTYEN"
BASE COUNT          708 a          345 c          249 g          779 t
ORIGIN      
        1 tgattatcgg catgcgcccc accatttaaa atattcatca taggtacagg taaagttttg
       61 gcatttgtac cacctacata acgatacagt ggtaagccag cttcttctgc agcagctttt
      121 gcaacagcca tacttactgc taaaattgca tttgcaccta atttgctttt attaggtgtg
      181 ccatctaaat caatcattac ttggtcaatt gcagcttgtt gtgctacatc atatcctact
      241 aatgcttttg atattgtatc atttacattt tttacagctt ttaaaactcc tttaccaaca
      301 tatttctttt tatcattgtc tctaagttct gcagcctcgt gtatgcctgt actagcacca
      361 cttggcacag cagctcttcc aaaataacca tcatcggtta atacatctac ttctactgtt
      421 gggttgccac ggctgtctaa aatttgtctt gcatggactt cgataatgta gctcataatt
      481 taggtttaat tttaaaaaaa ttggtgttga taagaaaaaa gtatagaatt taattttgca
      541 aaattatatt attttttgaa gttaattgct gaaagatatc ctctagtttt cctttatttt
      601 ttaaattttc cagtttatca tctgcaacaa tttctccacg gttaataaca ataattctat
      661 cgcatattgc ttctacttct tgtaagatat ggcttgaaaa taaaacagtt ttattctttc
      721 cttgttcttt aattacattt cttatttcaa ttatttgatt aggatctaag ccactcgttg
      781 gttcatctaa aattaatact tgtgggttat gaattaatgc tgcagccaaa cctacacgtt
      841 gtttataacc tttagataat tggccaattt ttttattttg ttcaatctgt aacccagtta
      901 attcaattac atgtaaaaca gccttagcag aatttgttaa ctgatgaatt tgagcaataa
      961 attttaaata ctcttttaca tacatatcta aataaagagc attgctttcg ggtaaataac
     1021 caatttgttt tttagttagt attgggtttt cctgcacatt aatttcatta ataaatgcat
     1081 taccaacagt tgcagctaaa taacctgtta atattttcat tgtagtgctt ttaccggcac
     1141 cattaggacc taaaaatcct acaatttctc ccttattaat attaaaattt atattattta
     1201 ctgctttttg agttccataa atttttgtaa ttccagcaac cttaattgac atttaaaata
     1261 atttatacag ttaaaaacat tggtaaaatt gaaaacaaaa taataagacc taacataaaa
     1321 tacattgcta cagtaagtaa attataaaag ctcatcaact tcttacgttt attcccttct
     1381 gccatatcca tatcatcttg attataccat ttaaatatag agtaaagcaa gccttgaaat
     1441 ccttctacat ttttattaaa gaaaaaagta agcaatattt taataccaat tactacagcc
     1501 attaaaagcc ataaataaat ataatattga ctaattagtt ttaataacat agaataaaac
     1561 acataactga ttattgttag aacaagttca tttcattgtt ttacaacaat catttaagct
     1621 actaaattgt agaaaattta aacaactgca aaaaagcatt tttttatctt taaaatcaag
     1681 aaaattaagc ttatttacat aagttttgca ttaactttat gtatgaaaat tttattattc
     1741 tacataaaga gaaaatatac ttgttatatt gtttcaattt tcttcaccat tttctctact
     1801 cctttatttt atagcacaac acaagctcaa acttttgctg aattgaacaa attgtttatt
     1861 tcattatatg ataacggtaa atacgattct gctgtattga ttggagaaaa agcattaatt
     1921 caatgcgaaa aagaatatgg taaaaaagat attaactacg ccatctcata tctaaacatt
     1981 gctgatgcct atgctgcatt aaaaaaaata tctgaagcgg tattaaacta tagtaaatct
     2041 attgaagtat ttgccattgt atataacacc tacgaaaaca t
//
LOCUS       DAOQHP010000378         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_41884_length_2062_cov_2.411061, whole genome shotgun sequence.
ACCESSION   DAOQHP010000378 DAOQHP010000000
VERSION     DAOQHP010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41884_length_2062_cov_2.411061"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLY81_10010"
     CDS             complement(<1..473)
                     /locus_tag="PLY81_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30603.1"
                     /translation="MKHIFFIILSFAVIILKAQSPGCPVITPIGATICQGQCANLTAT
                     AVTNYATTSYAVSSIPYSPFAYSGGTAVSVATDDVWSGAVNLPFNFCFFGTSYNQVWL
                     GSNGQICFTPKTAGGYDAYPVTVAGALPSSANTPGNTINVFRDINPASGGTVAYY"
     gene            complement(485..958)
                     /locus_tag="PLY81_10015"
     CDS             complement(485..958)
                     /locus_tag="PLY81_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30604.1"
                     /translation="MKNQLKTLTYILIILFSSHFSLLSQEQSAVSELLNRNEVAIYKA
                     QKEMIAHNAKTNIDKLALSVTLQVKAIDTYKKGSLNEASNYAIASRENALLILKDLLD
                     VKQLAYFELDNSEKNIVKTINYNPSTLNNKVEAYNEAILLDPIKIRETYNISINQ"
     gene            complement(1070..>2062)
                     /locus_tag="PLY81_10020"
     CDS             complement(1070..>2062)
                     /locus_tag="PLY81_10020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNF30605.1"
                     /translation="KQELQRKDAENKQAMAEAEIRSQQKLITLAVIGLIILSGLVLLV
                     FRGYKHKQKANEIISKQKELVEHKNKEIVDSINYAKYIQTALLPSDTMIKNLPFNCFV
                     LFKPKDIVSGDFYWLHEQIDTTTQNKELYIAAVDCTGHGVPGAMVSVVGNNGLNRCVK
                     EFNIHNTGKILDKLSELVEETFEKSEKELKDGMDISLLKLELIPKMPNKVNVQWSGAN
                     NPLWIIKKGTQNIEEIISNKQPVGKFFNKKDFTTHYFTFDKGDRLFIFTDGYADQFGG
                     PKGKKFMYKQFKEVLLNSSTQPLLEQKNALDKAFLNWKQDADQVDDVCIIAIEI"
BASE COUNT          654 a          343 c          315 g          750 t
ORIGIN      
        1 atataataag caacagtacc accagaagct ggattaatat ctctaaaaac atttattgta
       61 ttacctggtg tatttgctga gctaggtaaa gcgcctgcta cagttacagg ataagcatca
      121 taacctccag ccgttttagg agtaaaacaa atttgtccat tagaacctaa ccacacttga
      181 ttgtacgatg ttccaaaaaa acagaagtta aacggaagat ttaccgctcc actccataca
      241 tcgtctgtag caacagatac cgcagtacct cccgaataag caaaaggaga atatggaatt
      301 gaagatacag cgtatgatgt ggttgcataa tttgtaactg cagttgccgt taagttagca
      361 cattgccctt ggcaaatagt tgctccgata ggagtaatta ctggacaacc aggcgactgt
      421 gcttttaaaa taattactgc aaaacttaat ataataaaaa atatatgttt cataatctta
      481 tgaattattg attaatagaa atgttataag tttctctaat ttttatgggg tctaataaaa
      541 ttgcttcatt gtatgcttca actttattat ttaatgtaga agggttatag tttatagttt
      601 ttacaatatt cttttcagaa ttatctaact caaaataagc gagttgtttt acatctaaca
      661 agtcttttaa aattagtaag gcattctctc ttgatgcaat ggcataattt gatgcctcgt
      721 ttaaactgcc ttttttgtat gtatctattg cttttacttg tagagtaacc gacaatgcca
      781 gtttatctat attagtttta gcattatgag ctatcatttc tttttgtgct ttataaatag
      841 caacctcatt tcgattaagc aactccgaca ctgcgctctg ttcttgagaa agaagagaga
      901 aatgagaact gaaaagaata attaaaatgt aagtaagggt ttttagttga tttttcatat
      961 ctatagatta tatgataaat atacattatt aaggaaataa attaaactcc tataataaat
     1021 ggttttaggg attaactcag tgagaaaggt aattcgccaa ctaaatcttt tagatttcaa
     1081 tagctataat gcaaacatca tctacttggt ctgcatcttg tttccaattt agaaatgcct
     1141 tatcaagagc atttttctgt tcaagtaagg gttgtgtaga tgaattaaga agcacctctt
     1201 taaattgctt gtacatgaat tttttacctt ttggaccacc aaattgatca gcataaccat
     1261 ctgtaaaaat aaaaagacgg tcgcctttat caaatgtaaa ataatgggtt gtgaaatctt
     1321 ttttattaaa aaatttccct acaggttgtt tattagagat aatttcttca atattttgag
     1381 tacctttttt tattatccat aagggattat tggccccact ccattgaaca ttcactttat
     1441 tgggcatctt aggtataagt tctaatttta gtaatgaaat atccattcca tcttttagtt
     1501 ccttttcact tttctcaaaa gtttcttcaa ctaattcaga tagtttatca agtatttttc
     1561 ccgtattatg aatattaaat tcctttacac aacgatttaa tccattatta cctaccacac
     1621 ttaccatggc gccaggaaca ccgtgccccg tacaatctac tgcagcaata taaagttctt
     1681 tgttttgagt tgttgtatct atttgttcgt gtagccaata aaaatcgcca cttacaatat
     1741 ctttgggttt aaataaaaca aaacaattaa atggtaagtt tttaatcatc gtatcagatg
     1801 gtaacaaggc tgtttgaata tacttggcgt aattaatcga atctacaatt tctttgtttt
     1861 tatgttcaac taattctttt tgcttagata tgatttcatt tgctttttgt ttatgcttgt
     1921 aacccctaaa caccaaaagc actaagcccg aaagtattat taatcctatc actgctaagg
     1981 ttattaattt ttgctggctt ctaatttcgg cttcggccat ggcttgttta ttttcagcat
     2041 ctttgcgttg caactcttgt tt
//
LOCUS       DAOQHP010000379         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_42152_length_2055_cov_3.806000, whole genome shotgun sequence.
ACCESSION   DAOQHP010000379 DAOQHP010000000
VERSION     DAOQHP010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42152_length_2055_cov_3.806000"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLY81_10025"
     CDS             complement(<1..344)
                     /locus_tag="PLY81_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNF30606.1"
                     /translation="MNHTILSNSFLKLVTIMDELREQCPWDKKQTIHTLRPLTIEELY
                     ELTDAITNNNWQNIKEELGDVLLHILFYSRIAKEENKFLLHEVIETICEKLINRHPHI
                     YGNVKVENEEDV"
     gene            478..1311
                     /locus_tag="PLY81_10030"
     CDS             478..1311
                     /locus_tag="PLY81_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30607.1"
                     /translation="MKLLLSILTFCSISIQPLFAQYYFNDIVATNNTNQLQQLYVKQN
                     IKTIKTVSKESDGSIINDFFIMQSIHKNGNEITTQSQQSSGSKSVLKSFFENGKIVKT
                     INEEDGVSTTSIYSYIDNKLQSIVSGTTDTFITSNSKEEHIWFYKSNSTPEKMFKIKN
                     GKDTTVVNFISDENNLIIEEQWVRNGKMIENYYYYYNDKNWLTDIVRYNNKAKKMLPD
                     FLYEYDTEGRVSKMIQVIAGGTNYNIWVYTYNSLGLKEKEVCYDKKKQLLGTVEYLYN
                     N"
     gene            1319..>2055
                     /locus_tag="PLY81_10035"
     CDS             1319..>2055
                     /locus_tag="PLY81_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF30608.1"
                     /translation="MKNIKSILAKPFAAIVSKQVKKSVQNAVVDQKSIFTSLIKTAKN
                     TQFGNDHFFKDINNYATFANAVPIRDYEQFKNYIELIKEGKQNILWKGKPIYLAKTSG
                     TTSGVKYIPITKDSISNHINTARNALLCYMNETGNTNFAAGKMIFLSGSPVLERVGNI
                     PTGRLSGIVNHHVPQYLRTNQLPSYTTNCIEEWETKLEKIVEETIQKNMTLISGIPPW
                     VQMYFDKLTEKSGKTIAELFPNFSVMV"
BASE COUNT          765 a          274 c          301 g          715 t
ORIGIN      
        1 ttaacatctt cttcgttttc tacttttaca ttaccgtaaa tatgcggatg cctattaatt
       61 aatttttcgc aaattgtttc aataacttca tgtaataaaa atttattttc ttcttttgca
      121 attctgctgt agaataatat atgcaacaat acatcgccta attcttcttt aatattctgc
      181 caattattat tggtaattgc atctgtaagt tcatataatt cttcaatggt taatggcctt
      241 aaagtatgta tggtttgttt tttatcccaa gggcattgtt ctcttagttc atccattatt
      301 gtaactaact ttaaaaagct attacttaaa attgtatgat tcatagtatt tgcttaaaat
      361 tctttttgtt aatttaactt taaaagtact gtttttttaa ccacagtttt actaaacttc
      421 tattcatttt tgaatgttga atagcttgtt gctacttttg aggcagaaat atattttatg
      481 aaattattac tttcaattct tactttttgt tctataagca ttcaaccctt gtttgcacaa
      541 tattatttta atgatattgt tgctaccaat aatacaaacc aattacaaca attatatgta
      601 aaacaaaata ttaaaacaat aaaaacagtt agtaaagaaa gtgatggtag cataataaat
      661 gattttttta ttatgcaaag tattcataaa aatggcaatg aaattactac acaatcacaa
      721 caatcatcgg gtagtaaatc tgttttaaaa tctttttttg aaaatgggaa aatagtaaaa
      781 actattaatg aagaagatgg tgtttcaact acttccattt attcttatat tgataataag
      841 cttcaatcca ttgtatctgg tactacagat acttttatta catccaatag taaagaagaa
      901 catatttggt tttataaatc taactctact cctgaaaaaa tgtttaagat taaaaatgga
      961 aaagatacaa ctgtagttaa ctttatttct gatgaaaata atttgattat tgaagaacag
     1021 tgggtgagaa atggaaaaat gatagaaaat tattattact attataatga taaaaattgg
     1081 cttactgata ttgtaaggta taataacaaa gctaaaaaaa tgctacccga ttttttatat
     1141 gaatacgata cagaaggaag agtaagcaaa atgattcagg taattgctgg cggcactaat
     1201 tataacattt gggtttatac ttacaatagt ttagggttga aagagaagga agtgtgttac
     1261 gataaaaaaa agcagctatt aggcacagtt gaatacctat acaataacta attagcttat
     1321 gaaaaacata aaatctatat tagcaaagcc ttttgcagca atagtttcaa agcaagttaa
     1381 aaaaagtgtt cagaatgctg ttgtagatca aaaatctatt tttactagtc taattaaaac
     1441 tgcaaaaaac actcaatttg gcaatgacca tttctttaaa gacattaata attatgcaac
     1501 ttttgcaaat gctgttccaa ttagagatta cgaacaattt aagaattata ttgaacttat
     1561 taaagaaggc aagcaaaata ttttatggaa aggtaaaccc atttatttag ctaaaacaag
     1621 tggcaccaca agtggcgtaa aatatattcc cattacaaaa gattctattt ctaaccatat
     1681 taacacagca cgtaatgcat tgttgtgtta tatgaacgaa acgggtaata caaactttgc
     1741 tgctggtaaa atgatttttt taagtggaag ccctgtttta gaaagagttg gcaacattcc
     1801 aacaggtagg ttaagtggaa ttgtgaacca tcatgtacca caatatttaa gaacgaatca
     1861 actgccttct tatactacca attgtattga agaatgggaa acaaaattgg agaaaattgt
     1921 agaagaaact attcaaaaga acatgacttt aattagtgga attccgccat gggtgcaaat
     1981 gtattttgat aagttaacag agaaatcggg gaaaacaatt gccgaacttt ttccaaactt
     2041 tagtgtaatg gtgca
//
LOCUS       DAOQHP010000380         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_42381_length_2048_cov_4.221274, whole genome shotgun sequence.
ACCESSION   DAOQHP010000380 DAOQHP010000000
VERSION     DAOQHP010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42381_length_2048_cov_4.221274"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLY81_10040"
     CDS             <1..218
                     /locus_tag="PLY81_10040"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HNF30609.1"
                     /translation="NCIFRINRDVRFSPNKTPYKTNMGASINKGGKKIANAGYYLHIE
                     PGASFLAGGIWMPKTIELKKNKTRNRL"
     gene            502..1392
                     /locus_tag="PLY81_10045"
     CDS             502..1392
                     /locus_tag="PLY81_10045"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNF30610.1"
                     /translation="MFINLSEINLIERQAWLQHAIAPRPICFVSTTDKEGNINLSPFS
                     FFNLFSSNPPIVIFSPARSGRTGLLKDTFLNVQEVPEIVINICDFDMVQQVSLSSCEY
                     PKQVDEFIKAGFTKVQSTMVKPPMVKESKINLECKVIETKSLGEGGGAGQLVFAEVLC
                     MHINESILNEDKSMIDHQKMHHIARLGGDYYASITPENLFTVPKPIRELGIGVDALPE
                     GIRNSTILTGNNLGMLANVQSLPQVDATFTDETLKNIVQYFSASPNEMEKELHNYAKE
                     LLDNKKVKEAWQVLLTLENN"
     gene            complement(1394..>2048)
                     /locus_tag="PLY81_10050"
     CDS             complement(1394..>2048)
                     /locus_tag="PLY81_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30611.1"
                     /translation="FRVNLYYANGNSENYIGRIVGFSDRISSVLVPQSFMDWANKKYG
                     NSKTTNPGRVVIKTKDPGNPELVKFLKSKGLTTDADKTRFSKYRQIVNAVVNVSWVTG
                     AIMLLFALLVFTMFIQLTVASCKEEIKLLITIGTSPIQLKSFLMKQFFPSNIVIVGLA
                     LMLVSIVQFMLYKYLQAQNIFLSAYISIYTIVAACIVLMLLWLVNHFTIKKYIEVKV"
BASE COUNT          774 a          300 c          325 g          649 t
ORIGIN      
        1 aaaattgtat ttttagaata aacagagatg ttcgttttag cccaaacaaa acaccctaca
       61 aaaccaatat gggtgcaagt attaataaag ggggaaagaa aatagccaat gctggttact
      121 atttacatat tgaacctggt gcaagttttt tggctggtgg aatttggatg ccaaaaacta
      181 ttgaattaaa aaaaaataag acaagaaata gattataact ttttagagtt taataaaata
      241 gttgcatccc aaaaaattat tcaacaattt ggtgggattg ataaaagcaa ggaatatgtt
      301 ttaactcgtc caccaaaagg atatgatata gacaatcccg ctattgaata tttaaagttt
      361 aaaagttata ttgtatctaa aaatatttta gacgaagagc tacttaaaga aaattgtatt
      421 caaaatattg ttagtaattt tgaaactata acaccattaa tatattttat caataaagca
      481 atagaagatt aaaaacattt tatgtttatc aatttatctg aaattaattt aatagaacga
      541 caagcatggt tacaacatgc aattgcacca cgccctattt gttttgtaag cacaacagat
      601 aaagaaggga atataaacct tagtcctttt tctttcttca atttattttc ttccaatccg
      661 cctattgtaa tattttctcc tgcccgtagc ggtagaactg gattactgaa agatactttt
      721 ttaaacgtac aagaagtgcc agaaattgta attaatattt gtgattttga tatggtacaa
      781 caagtaagcc ttagcagttg tgagtatccc aaacaggtag atgagtttat taaagctggt
      841 ttcacaaaag tacaatctac tatggttaaa cctccaatgg ttaaagagtc taaaataaat
      901 ttagaatgta aagtaattga gacaaaaagt ttaggagaag gtggtggtgc tggtcagttg
      961 gtttttgcag aagtattgtg tatgcacatt aacgaaagca ttttaaatga agataaaagc
     1021 atgattgacc atcaaaaaat gcatcatatt gcaaggcttg gaggagacta ttatgcaagc
     1081 attacaccag aaaatttatt cactgttcca aaaccaatta gagagttggg cattggtgta
     1141 gatgctttgc cagaaggtat aagaaatagt acaatactta caggtaacaa tttaggcatg
     1201 ttggctaatg tgcaaagttt accacaggtt gatgcaacat ttactgatga aactttaaaa
     1261 aacattgttc aatatttttc tgcaagccct aacgaaatgg aaaaagaatt acacaattat
     1321 gccaaagagt tattagataa taaaaaagta aaggaggctt ggcaagtatt attaactcta
     1381 gaaaacaatt aacctacact ttcacttcaa tatatttttt aatggtaaaa tggtttacca
     1441 gccataacaa cattaaaaca atacaggctg caacaatagt ataaatagaa atataagcag
     1501 acaagaaaat gttttgtgct tgtaaatatt tatatagcat aaattgtaca atagaaacaa
     1561 gcattaatgc taaaccaaca attacaatat tagaaggaaa aaattgtttc attaaaaagc
     1621 ttttcaattg tattggcgat gtgccaatag taattaataa ttttatttct tctttacacg
     1681 aggcaacagt aagctggata aacattgtaa atacgagtaa tgcaaacaaa agcataatag
     1741 caccagttac ccaacttaca ttaacaactg catttacaat ctgcctatac ttactaaatc
     1801 tggttttatc tgcatcggtt gttaaacctt tgctttttag aaattttact aattctggat
     1861 tgccaggatc tttggttttt atcacaactc tacctgggtt agtagttttg gaattgccat
     1921 attttttgtt ggcccaatcc ataaaactct gtggtaccaa tacactcgaa attctatcac
     1981 taaaaccaac aattctacca atataatttt ctgagtttcc attggcataa taaagattaa
     2041 ccctaaaa
//
LOCUS       DAOQHP010000381         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_42561_length_2043_cov_4.700201, whole genome shotgun sequence.
ACCESSION   DAOQHP010000381 DAOQHP010000000
VERSION     DAOQHP010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42561_length_2043_cov_4.700201"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLY81_10055"
     CDS             complement(<1..1243)
                     /locus_tag="PLY81_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30612.1"
                     /translation="MQIILFLLYLILFCFALTRIKFFTNSNLEKPILISLFLIKVLAG
                     LAYAWFYTLPAYHINSDSFRFYAYSLEETNILLTQPLHFFKDIFTYGYQTTGNVFVGD
                     NSYWNDLKSNIIIKLLAVCNVFSFKNYYINIIFFNFLFFFGLIAFYRLMQNVFNVNKV
                     ILILPIFFIPSFLFWCSGIHKDGLLFSAIGMVFFYFNVCLLKGYSFKKVALVLSLLFI
                     IFFLRSYVALLLLLAMFAWWVAQQSKKHLLSFILVYVVAIILFFISPFIHSKLNFPNY
                     ISVKQHEFSILDGGSSIIAKSIDPSFAGFLHYLPNALDMAFMQPHIFNFKNLSYIPAI
                     IENLGMIIILIILLVKRKKKIVMPPVVYACLFFGITMLLIAGYTVTFSGAIVRYRSLV
                     LPILVTLVFANINFESIFKNMN"
     gene            complement(1307..>2043)
                     /locus_tag="PLY81_10060"
     CDS             complement(1307..>2043)
                     /locus_tag="PLY81_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNF30613.1"
                     /translation="YGNNEAIAMSSIKSFALQSNNVSSTSINVGKSLEEMYDETSTNI
                     INATAKESFEAIKILKNAKIENYQPENGAVYPNSDLGKSLNQIAQLIKMNVGLEVAFA
                     EHGGWDTHFNQGKETGIFARNAEDLSNSIFALWTDLEKFQDDVVIMTMTEFGRTVKQN
                     GTGGTDHGRASCNFIIGNKISGGRVFGNIQPLAVENLEDGRDLAVTTDFRNVFSEVAT
                     KHLNIINHQALFPSFEEKYLNITKKV"
BASE COUNT          794 a          313 c          247 g          689 t
ORIGIN      
        1 aattcatatt cttaaaaata ctttcaaaat taatattggc aaaaactaat gtaactaaaa
       61 ttggtaaaac caagcttctg taacgtacta ttgctccaga aaatgtaact gtatatcctg
      121 caattaataa catagtaata ccaaaaaata aacaagcata aacaacaggt ggcataacaa
      181 tttttttctt tcttttaacc agcaggataa ttaatattat tatcattcct aggttttcaa
      241 ttatagcagg tatataactt aaatttttaa aattaaaaat atgaggttgc ataaaagcca
      301 tatctaaagc attaggcaaa taatgtaaaa aacctgcaaa actaggatca atgctttttg
      361 caataatgga actacctcca tctaagattg aaaattcatg ttgttttaca gaaatataat
      421 taggaaaatt tagttttgaa tgtatgaatg gtgatataaa gaaaagaatg attgcaacaa
      481 cataaaccaa gataaagctt aataaatgtt ttttagattg ttgggctacc caccatgcaa
      541 acattgccaa caacaacagc aaagcaacat aacttcttaa aaagaaaata ataaaaagta
      601 aagacaatac aagtgctact tttttaaaac tatatccttt taataaacat acattaaaat
      661 aaaaaaatac cataccaatt gcagaaaaca ataatccatc tttatgaata ccactgcacc
      721 aaaacaaaaa ggatggtata aaaaaaattg gaagaatgag tattacttta ttaacgttaa
      781 aaacattttg cattaaccta taaaaagcaa tcaatccaaa aaagaaaagg aaattaaaaa
      841 atataatatt gatataataa ttcttaaaac taaaaacatt gcatactgct aataatttaa
      901 taataatatt actctttaaa tcattccaat aagaattatc gcctacaaaa acattaccag
      961 ttgtttggta accataagta aaaatatctt taaaaaaatg taatggttgg gtaagtaaaa
     1021 tatttgtttc ttctaaactg taagcataaa aacgaaaact atcgctattg atgtggtatg
     1081 caggaagtgt gtaaaaccaa gcataagcaa gcccagcaag aactttaatt aagaataaac
     1141 taattaatat gggtttctct aaattagaat tagtgaaaaa ctttattctg gttagtgcaa
     1201 aacagaacaa tattagatac aataaaaaaa gtataatttg caagtataaa atttggagca
     1261 atttaagaaa aactgcatta taaatttttt atactattat ttaagtttac actttttttg
     1321 taatgtttag atacttttct tcaaaacttg ggaataaagc ttggtgatta attatgttta
     1381 aatgtttagt tgcaacctcg ctaaaaacat ttctaaaatc tgttgttact gcaagatctc
     1441 ttccatcttc taaattctct actgctaaag gctgtatatt accaaatact cttcctccag
     1501 atattttatt gccaataata aaattacaac tagctcttcc atgatctgta cctcctgtac
     1561 cattttgctt tactgttctt ccaaattcag tcatagtcat aataactaca tcatcttgaa
     1621 atttttccaa atcagtccat aatgcaaata tgctattgct aaggtcttct gcgtttctag
     1681 caaaaatgcc cgtttctttt ccttggttaa aatgtgtatc ccaaccacca tgttcagcaa
     1741 atgcaacttc taatccaaca ttcattttaa ttaactgagc aatttggttt aagcttttac
     1801 ctaaatcaga atttggatat acagccccat tttctggttg ataattttct attttagcat
     1861 tttttaaaat tttaattgct tcaaaacttt cttttgcggt tgcattgata atatttgtag
     1921 atgtttcatc gtacatttct tctaagcttt tacctacatt gatagaggta gatgacacat
     1981 tgttggattg taacgcaaaa cttttaatgc tactcattgc aatggcttca ttatttccat
     2041 aaa
//
LOCUS       DAOQHP010000382         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_42834_length_2036_cov_3.228168, whole genome shotgun sequence.
ACCESSION   DAOQHP010000382 DAOQHP010000000
VERSION     DAOQHP010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42834_length_2036_cov_3.228168"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..493)
                     /locus_tag="PLY81_10065"
     CDS             complement(197..493)
                     /locus_tag="PLY81_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNF30614.1"
                     /translation="MLQTDICIIGAGPAGATTSLFLSKMQIPHIIVDASVFPRDKICG
                     DALDLKVMRILNCLENDFVKNEILHNHNFSKTYGVKINISENKSKQWALTEIAV"
     gene            complement(536..1276)
                     /locus_tag="PLY81_10070"
     CDS             complement(536..1276)
                     /locus_tag="PLY81_10070"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HNF30615.1"
                     /translation="MFGLFKKNKTHPDLSFIGVDMHSHLLPGLDDGLKTIEDTIAFVQ
                     ELNTLGYEKLICTPHILSAVHPNTPQTILPKLEEVQKAVKEKNIKIQIEAAAEYMVDH
                     EFEQLIKSGNKLLTFGTNFILIEMSYLAPSPNLGQVIFDLRLAGLQPILAHPERYNYY
                     HHQFHEFKKIKDTGCWFQVNMLSLAGYYGKHIKKTAEKLIAEGMIDLIGTDMHHQNHL
                     HATKQLALSNYFYKILKDVPLKNRMFLD"
     gene            complement(1286..>2036)
                     /locus_tag="PLY81_10075"
     CDS             complement(1286..>2036)
                     /locus_tag="PLY81_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNF30616.1"
                     /translation="VDTMVLVSTDKAINPTNVMGASKRIAEMYCQALQNRSELPIWDS
                     GIVKMGNHQLKRTKFITTRFGNVLGSNGSVIPRFQEQITNGGPVTVTHPDIIRYFMTI
                     PEACSLVLEAGTMGKGGEVFLFDMGEPIRILDLAKKMIRLAGLTPGKDIELRFTGLRP
                     GEKLFEELLNKEEEVIPTHNKKILIAKVIEFEFSKVSESINMLISLANQNQDEDVVKQ
                     MKFIVPEFISNNSIYESIDAQMFKPLYSAIR"
BASE COUNT          669 a          317 c          289 g          761 t
ORIGIN      
        1 aatagtagta gtaatagtag tagtaatagt agtagtaata gtagtagtaa tagtagtaat
       61 agtaaataag ataaccaaaa tattcgacta tatgattaat gtttttaatt tttatattaa
      121 aaagattgat aaggacaaaa aaaagaatga aaaaataaag atttttgttt taattttatt
      181 aattttttat ctttatttaa actgctattt cagttaatgc ccattgttta cttttatttt
      241 ctgaaatatt aatttttaca ccgtatgttt tagagaaatt atggttatgc agtatttcat
      301 tttttacaaa atcattctct aagcaattta aaatacgcat tactttaagg tctagagcat
      361 ctccacaaat tttatctcta ggaaaaacag aagcatctac aattatatgc ggtatttgca
      421 tttttgataa aaacaaagaa gttgtagcac ctgctgggcc tgctccaata atgcaaatat
      481 ctgtttgtag cattttaaat gatgacgtta aatttagagg caaataaata aaactttaat
      541 caagaaacat tctatttttt aagggcacat cttttaaaat tttatagaag tagttactca
      601 atgccaattg ttttgttgca tgtaaatgat tttgatggtg catatcggta ccaattaaat
      661 ctatcatacc ttcggcaatt agtttttctg ctgttttttt tatgtgtttg ccataatagc
      721 cagccaacga taacatattt acttgaaacc aacagccagt atcttttatt tttttaaact
      781 catgaaactg atggtgataa taattatatc tttctgggtg tgctaaaata ggttgcaaac
      841 ctgctaaacg taaatcaaaa ataacttgcc ctaagtttgg agagggtgct aaataggaca
      901 tttcaatcaa tataaaatta gtaccaaaag ttaaaagctt attaccgctt tttattagtt
      961 gctcaaattc gtgatcaacc atatattctg cggctgcttc aatttgtatt ttaatatttt
     1021 tttctttaac tgctttttgt acttcttcta attttggtaa aatagtttgt ggggtattgg
     1081 ggtgcactgc agataaaata tgaggagtgc aaattaattt ttcataaccc aaagtattta
     1141 actcttgtac aaatgcaatg gtatcttcta tagtttttaa accatcatct aacccaggaa
     1201 gtagatgaga gtgcatatca acaccaataa acgataaatc ggggtgtgtt ttattttttt
     1261 tgaacaatcc aaacatgcag caaatttacc taatagcaga atataaaggt ttaaacattt
     1321 gtgcatctat gctttcataa atagagttat tactaataaa ctcaggaact ataaatttca
     1381 tttgtttaac aacatcttca tcttggtttt ggtttgctaa acttataagc atattaatgc
     1441 tttcagaaac tttagaaaat tcaaactcta ttactttagc aattaaaatt ttcttgttat
     1501 gtgtcggaat tacttcttct tctttattca ataattcttc aaatagtttt tctccaggtc
     1561 ttaaaccagt aaatcttagt tcaatgtcct tacctggtgt taaaccagct aatcgaatca
     1621 tttttttagc caaatctaaa attctaatgg gttctcccat atcaaataaa aacacttcgc
     1681 cacctttgcc cattgtacct gcttctaaca ctaaagagca agcttctggt atggtcataa
     1741 aatatctaat aatatctgga tgtgtaacag tgactggacc tccattagtt atttgctctt
     1801 gaaaacgtgg aattacagaa ccattagagc ctaatacatt tccaaatctt gttgttataa
     1861 attttgttct ttttaactgg tggttaccca tttttacaat tccactatcc catattggta
     1921 gctcgctcct attttgtaaa gcctggcaat acatttctgc aattctttta gatgccccca
     1981 ttacattggt tggattgata gccttatctg ttgaaaccaa caccattgta tctacc
//
LOCUS       DAOQHP010000383         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_42961_length_2033_cov_3.205258, whole genome shotgun sequence.
ACCESSION   DAOQHP010000383 DAOQHP010000000
VERSION     DAOQHP010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42961_length_2033_cov_3.205258"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLY81_10080"
     CDS             <1..439
                     /locus_tag="PLY81_10080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNF30617.1"
                     /translation="INAGKETVTLIKGAALFDSAESFGMIRAGKIDLTVLGAMEVSEK
                     GDIANWKIPGKMVKGMGGAMDLVASAQNIIVAMMHTNPKGESKLLPSCTLPLTGLGCV
                     KKVVTDLAVLNITQDGFELIETAPGVTIDEVKAKTAGKLIVKM"
     gene            453..>2033
                     /gene="pgi"
                     /locus_tag="PLY81_10085"
     CDS             453..>2033
                     /gene="pgi"
                     /locus_tag="PLY81_10085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNF30618.1"
                     /translation="MLSKINPTTTQAWLLLTKHFEAEMNHASMRKLFAENENRFQEFS
                     IIDGDILFDYSKNIINNKTIQLLLQLANECKLQEAINEMFSGAKINETENRAVLHTAL
                     RNFSDTPIYNDGEDVMPSIRKVLKQMETFCNKVHKKEWLGYTGKPIKYIVNIGIGGSD
                     LGPVMVTEALKPYWINSIETYFVSNVDGTHIAETLRKINAEETLFLIASKTFTTQETM
                     TNAHTARSWFIEFAKNEKYVAKHFIALSTNEQEVKKFGIDTANMFEFWDWVGGRYSLW
                     SAIGLSIALTIGYKNFEQLLQGAFDIDNHFKTENHSKNIPVIMALIGIWYTNFFNAKS
                     EAILPYDQYMHRFAAYFQQGNMESNGKNTDRNGNEINYATGPVIWGEPGTNGQHAFYQ
                     LIHQGTQMIPCDFIAPVKSHNPLSDHHTKLLSNFFAQTEALMNGKTLNEAKEELIKAG
                     KNEEEIKKLLAFKVFNGNKPTNSFLIKKITPFTLGQLIALYEHKIFVQGVIWNIFSFD
                     QWGVELGKQLANKILPELN"
BASE COUNT          762 a          281 c          365 g          625 t
ORIGIN      
        1 tataaatgct gggaaagaaa cagttacttt aattaaaggt gctgctttat ttgatagtgc
       61 agaaagtttt ggtatgatac gagccgggaa aatagacctt accgttttag gagcaatgga
      121 agtaagtgaa aaaggagaca ttgctaactg gaagatacca ggcaaaatgg ttaaaggcat
      181 gggtggtgca atggatttag ttgctagtgc tcaaaatata attgttgcaa tgatgcatac
      241 taaccctaaa ggagaaagta aattattgcc atcatgcaca ttacccttaa ctggtttagg
      301 ttgtgttaaa aaagttgtta cagatttagc tgttttaaat attacacaag atggttttga
      361 attaatagaa actgcaccag gagtaactat tgatgaggtt aaagctaaaa ctgcaggtaa
      421 actaattgta aaaatgtaga ttaaacaatt ttatgctttc taaaataaat ccaactacta
      481 cacaagcctg gttattgctt acaaaacatt ttgaagcaga aatgaaccat gcaagcatga
      541 ggaaactttt tgcagaaaat gaaaatagat ttcaggaatt ttcaattatt gatggggata
      601 ttttatttga ttattctaag aatataatta ataacaaaac cattcagctt ttattacaat
      661 tagccaatga atgtaaactg caagaagcta ttaacgaaat gttttcaggt gctaaaatta
      721 acgaaaccga aaaccgtgca gttttacata ctgcattaag aaatttttct gatacgccaa
      781 tttacaatga tggtgaagat gttatgccat ccatcagaaa ggttttaaag caaatggaaa
      841 ctttttgtaa caaagtgcat aaaaaggaat ggttgggtta tacaggaaaa cctattaaat
      901 atattgtaaa cattggaatt ggtggcagcg atttaggccc tgttatggta actgaagctt
      961 taaaaccata ctggattaat tctattgaaa cttattttgt aagcaatgtt gatggtacac
     1021 atattgcaga aactttaaga aaaattaatg cagaagaaac tctattttta attgcatcaa
     1081 aaacatttac tactcaggaa accatgacta atgctcatac tgctcgttca tggtttattg
     1141 aatttgcaaa aaacgaaaag tatgttgcaa aacattttat tgcacttagt actaatgaac
     1201 aagaggtgaa aaaatttggt attgatactg ccaatatgtt tgaattttgg gattgggttg
     1261 gaggaagata tagcctttgg agtgcaatag gtttatccat tgcattaacc attggttaca
     1321 aaaattttga acagctttta caaggtgcat ttgatattga taaccatttt aaaaccgaaa
     1381 atcattcaaa aaatattcca gtaataatgg cattaattgg tatatggtac actaattttt
     1441 ttaacgctaa aagcgaagcc attttacctt acgaccaata tatgcacagg tttgctgcct
     1501 attttcagca aggaaatatg gaaagtaatg gaaaaaatac tgatagaaat ggaaatgaaa
     1561 ttaactatgc aacaggccca gtaatatggg gcgaacctgg caccaatggt caacatgcat
     1621 tttaccaatt aattcatcag ggcacacaaa tgataccatg cgattttatt gcacctgtta
     1681 agagccataa ccctttaagc gatcatcata caaagctttt aagtaacttt tttgcacaaa
     1741 cagaagcttt aatgaatggt aaaactctaa atgaagcaaa agaagaacta ataaaagcag
     1801 gaaaaaatga agaggaaata aaaaaattac ttgcttttaa agtttttaat gggaataagc
     1861 caaccaattc ttttttaatt aaaaaaatta ctccattcac tttaggtcag ctcattgctt
     1921 tatatgaaca taaaattttt gtacaaggag ttatttggaa catatttagt tttgaccaat
     1981 ggggtgtaga attaggcaag caattagcca ataaaatttt accagaatta aat
//
LOCUS       DAOQHP010000384         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_43632_length_2015_cov_4.665816, whole genome shotgun sequence.
ACCESSION   DAOQHP010000384 DAOQHP010000000
VERSION     DAOQHP010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43632_length_2015_cov_4.665816"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..>2015)
                     /locus_tag="PLY81_10090"
     CDS             complement(199..>2015)
                     /locus_tag="PLY81_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNF30619.1"
                     /translation="YDAVLSWGDNQINSGLPPNNGPDNFRFIFTSSTTGSAGGTGPAI
                     GQDGLEGIRLTPTSSNGVYTGIGGSPSVNPYTTGTNAGNTLEINSWGATNVPGGSSGL
                     RFTNLNTTSPTTPNPGYGVLAVNTNGDVIYVNAQGGNFGADCASTTNAGLLPNDWHVG
                     MNGHSVNFEGIGNVNIGDLQNCIPGSTSPARLWVRNTFGPPNPTDGFRVDNGAPSYAA
                     TLMGNVFIGGSGNSTWPGLVFSDRNLKENIINITSSMEIIQKLKPVSYNLKASTLPQL
                     TVDVTKTYGLIAQDVETIIPELVKEVVVPASYDSLGAVTNPSITVKSLNYTGLIPFTI
                     GAIQEVNVKQQEMQANLDKLNLSDAQVKTNINSFNALATIKTLNPVKYNFTNVNVPQL
                     TFKPNTDYGFVAQQLETVYPELVDTITVAATYDSLGAVVNPSKVLKTVNYKAMSALLV
                     RSIQEQQLIIDSLRNAQSKQDSINQAVQQQLANLASQISSCCSSNSAKTANNASTYVL
                     NQMDVELSDKDAIVLNQNVPNPFAEQTTITYNVPSSVGKAQLLFYNSAGQIIQTVDIK
                     TRGKGKVNVFAADLSSGMYHYTLVADGQVVDSKKMVRE"
BASE COUNT          603 a          385 c          311 g          716 t
ORIGIN      
        1 taaggagcct tgattttttt gtaacttttt gtatcaagac aaaaagtttt ataaagaact
       61 tgaacttttg tttcttttgc ttcaaggcaa aagaaagtaa aactaaattc aatatactca
      121 cagcttcatc tatgaaaaac aaaaaaaacc gcagctattc actgcggttt ttttattggg
      181 gtaaattgta aacgtttttt actctcttac cattttttta ctatccacaa cttggccatc
      241 ggctactaag gtgtaatgat acataccgct tgataaatcg gcagcaaata catttacctt
      301 gcctttgcct cttgttttaa tatctacagt ttgtataatt tgccctgcgc tgttataaaa
      361 taatagttgc gctttgccta cactacttgg tacattgtag gtaatggttg tttgctcggc
      421 aaacggatta ggtacatttt gatttaatac aatagcatct ttatcactca actctacatc
      481 catttggttt aatacatatg tgctggcgtt attagctgtt ttagcactgt tgctagaaca
      541 acaagaacta atttgtgaag ctaaatttgc tagttgttgt tgaacagcct gattaatgct
      601 atcttgttta gattgtgcat ttcttaaact atcaataatt aattgttgct cttgtatcga
      661 acgaactaac aaagctgaca tcgctttata attaactgtt tttaatactt ttgaaggatt
      721 aaccacagca cctaagctat cataagttgc agcaactgtt attgtatcca ctaattctgg
      781 atacacagtt tctaactgtt gtgctacaaa accataatct gtattaggtt taaatgttaa
      841 ttgaggcaca ttcacattcg taaagttata ttttactgga tttaaggttt tgatagttgc
      901 taacgcatta aatgaattaa tgttagtttt aacttgtgca tcgcttaaat ttaacttatc
      961 taaatttgct tgcatttcct gttgttttac atttacctct tgtattgctc ctattgtaaa
     1021 aggaattaac cctgtataat taagagattt tactgttata gacggattgg ttacagctcc
     1081 taaagaatca taactagctg gaacaacaac ctcttttact aactctggta taatagtttc
     1141 aacatcttga gcaatcaaac catatgtttt tgttacatca acagttaatt gaggtaacgt
     1201 tgaagctttt agattataac ttactggttt taacttttgt attatttcca tagatgaagt
     1261 tatatttatg atattttctt ttaaatttct atcagaaaaa accaatcctg gccatgtaga
     1321 attaccactt ccgccaataa atacgttacc cattaatgta gcagcatagc ttggagctcc
     1381 attatctacc ctaaatccat ctgttggatt tggcggtccg aaagtatttc taacccataa
     1441 acgcgcagga ctagtagaac cagggataca attttgtaaa tctccaatat ttacattgcc
     1501 aatgccttca aaatttacac tatgaccatt catcccaaca tgccaatcat ttggtaataa
     1561 cccagcgttt gttgttgagg cacaatcagc cccaaaatta ccaccttgtg cattaacata
     1621 aattacatct ccatttgtat taactgctaa cacaccataa cctggattag gagttgtagg
     1681 cgatgttgta tttaagtttg taaatcttaa ccccgagctt cctcctggca cgttggttgc
     1741 tccccatgaa ttaatttcta atgtgttacc tgcatttgta cccgtggtat atgggtttac
     1801 acttggcgaa ccacctatgc ctgtatatac tccatttgat gatgtgggtg ttaaacgaat
     1861 cccttctaat ccatcttgcc ctattgcagg tcctgtacct ccagcagaac cagttgttga
     1921 actagtaaaa ataaatctaa aattatcagg cccgttgttc ggaggcaaac cactattaat
     1981 ttgattatca ccccaactta atacagcatc atatc
//
LOCUS       DAOQHP010000385         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_43645_length_2015_cov_3.042857, whole genome shotgun sequence.
ACCESSION   DAOQHP010000385 DAOQHP010000000
VERSION     DAOQHP010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43645_length_2015_cov_3.042857"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2015
                     /locus_tag="PLY81_10095"
     CDS             <1..>2015
                     /locus_tag="PLY81_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF30620.1"
                     /translation="NNTTSISKNGAYQDFSCTNATIVDALQTYPLTISVKSFASSAQR
                     YEAYLDYNNNGIFEYPNELVASGTGVINQNKSYLHNVTIPDSAVKGKIIRLRVMADVH
                     AFSDNKRNCTQVLDLGDVEDYGVYINGTPTPPTVCPPGNVTLTTQAQINSFPINYNNC
                     KQISGNLTITGPSDITNLNGLSNLETITGQLNIVHNNNLTDLSGLSNLKTVGNNFWIS
                     DNTALTTINGLNALETTGQFYIGNNAALTTINFSNTLTGLTSFTIVNNPLLQSINGFN
                     NITSISGGLNINNNTVLQNFNAFSKLSSVGNLLQFSDNAQLQNIDSLHKLTTVGGAFN
                     IAKNGALTHLNGFRKLTSVGGFFQLWDNDNLVNIDSLYKLTNVGAELIVEENPQLSNI
                     TALAGVNPNTIGGTYGLYIVNNTSLSVCNLPNFCTYLANPSNTHPRTISGNLTNCLNE
                     AAVVAACTAPTNEYVNFLGTMFVQKGASGTGSSWSNAIGELADALVIAKSINDTSAGK
                     VKEIWVTSGTYKPMYSPEDGANFGTNQGRNNAFSLLNNVKLYGGFAGSESSFSQRILD
                     TANPTILSGDIDNNNLLDNGNTYHVLVAAGVSSSTVLDGFTIKMGYASSTGSLVVNGN
                     SVERRVGGGIINYSSSPIIRNCIITQNRTDYDGGGMANYFSSNPTIT"
BASE COUNT          701 a          305 c          339 g          670 t
ORIGIN      
        1 aataacacta catccatttc aaaaaatgga gcttatcagg atttttcatg tacaaatgct
       61 accattgtgg atgcattaca aacctatcca ttaaccattt cagttaaatc gtttgcaagt
      121 tctgcacaac gttatgaggc ttatttagat tataacaata atggcatatt cgaatatcct
      181 aatgaattag ttgcttctgg tactggagtt atcaatcaaa ataaaagcta tttacacaat
      241 gttacaattc cagattctgc tgttaaagga aaaattattc gtttaagagt tatggctgat
      301 gtacatgcat ttagtgataa taaacgcaac tgtacacaag ttctagattt aggagatgta
      361 gaagattatg gtgtttatat taatggaact ccaacaccac caactgtatg ccctcctgga
      421 aatgtaactt taactactca agcacaaatt aatagctttc ctattaatta taataattgc
      481 aaacaaataa gtggcaactt aactattaca ggaccttcag atattacaaa tcttaatggt
      541 ttaagtaatt tagaaacgat tacaggacaa cttaatattg tacataacaa taacttaact
      601 gacctaagtg gattatccaa tttaaaaaca gttggaaata atttttggat ttcagataat
      661 acagcattaa ctacaattaa tggtttgaat gctttagaaa caacagggca attttatatt
      721 ggaaataatg cagctttaac tacaattaat ttttctaaca cattaacagg tctcactagt
      781 tttaccattg taaataatcc cttactgcaa agtataaatg gattcaacaa tataacaagt
      841 attagcggcg gtttaaatat taataacaat acagttctgc aaaattttaa tgctttcagt
      901 aaactcagtt ctgttggtaa tttattacaa ttttcagaca atgctcaact tcaaaatata
      961 gacagtttac ataaacttac tactgttggt ggtgctttta atattgctaa aaatggagca
     1021 ttaacgcatc ttaatggctt tagaaaactt acttctgttg gtggattttt tcaactatgg
     1081 gataatgaca atctggtaaa tatagacagc ctttataaac tcactaatgt tggtgctgag
     1141 cttattgtag aggaaaatcc acagttgtca aatattacag ctcttgcagg agtaaaccct
     1201 aatactatag gcggcacgta tggtttatat atagtaaata atacaagtct ttctgtatgt
     1261 aatcttccta atttttgtac ttacttggca aacccttcca atactcatcc aagaacaatt
     1321 tcaggtaatc ttacgaattg tttaaatgaa gcagcagttg tagcagcttg cactgcacct
     1381 acaaatgagt atgtgaattt tttaggtacc atgtttgtac aaaaaggagc atctggtaca
     1441 ggaagcagtt ggagtaatgc tattggtgag ttggcagatg cattggtaat tgcaaaaagc
     1501 attaatgata caagtgctgg aaaagtaaaa gaaatatggg ttacaagtgg tacttataaa
     1561 cctatgtata gcccagaaga tggtgctaat tttggaacaa atcaagggag aaacaacgct
     1621 ttttctttat tgaataatgt aaaattatat ggcggttttg caggttctga atcttcattt
     1681 tctcaaagaa ttttagacac tgctaatcca actattttaa gtggagatat agataataat
     1741 aatttactgg ataatggtaa tacataccat gttttagttg ctgcaggagt atccagctct
     1801 actgtgttag atggttttac tattaaaatg gggtatgcaa gttctactgg gtctttagtt
     1861 gtaaatggta attctgttga aagaagagtt ggtggtggaa taattaacta ctcatcttct
     1921 cctataatta gaaactgtat aattacacaa aatagaactg attatgatgg aggaggtatg
     1981 gctaattatt tttcatccaa cccaacaatt actca
//
LOCUS       DAOQHP010000386         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_44356_length_1997_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAOQHP010000386 DAOQHP010000000
VERSION     DAOQHP010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44356_length_1997_cov_2.500000"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..802
                     /locus_tag="PLY81_10100"
     CDS             218..802
                     /locus_tag="PLY81_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF30621.1"
                     /translation="MKTLTHASDTQLIHAFQERNANEALEVLINRYKDKIFSSILFLV
                     KDKYLAEDLFQDVFIKVIDTLRNNKYSEEGKFLPWALRISHNLCVDYFRKVKRTPTIK
                     TSDDRDIFETINVVEESSETKLIANQSHDRVRKMIDMLPDEQREVLVLRHFADLSFKE
                     IADITNCSINTALGRMRYGLINLRKLMLEKQIAL"
     gene            complement(887..>1997)
                     /locus_tag="PLY81_10105"
     CDS             complement(887..>1997)
                     /locus_tag="PLY81_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30622.1"
                     /translation="KGLWVSEFRVESGLNCGGHAFATDGLLTGPILEEFKQNRNAMLN
                     ELFEMYQKALETKNINCNVCPTQKITYQGGIGNHEEDIFLQQFYQLDGTGWGSPFLLV
                     PEATTVDEATLNNLAKATKDDFYISGSSPLGVPFNNFKLSSSEKQRIERIEKGRPGSP
                     CIKKLLVSNTEFTKEPICTASRQYQNLKIKQLQEQGLNQEEYQEQFNIITEKACLCEG
                     LATAAYINNDIVEKNESTAVAICPGPNTAYFNKIYSLNDMVKHIYGKINLLDGVKRSN
                     MYINELHLYIEYIKKDINHSIKNLNDKKIKYYNKFKEQLENGITYYKNIIPQIFNQSE
                     AYKKQMLTELDEAVNKINQIFSILSNTEEKQTKVA"
BASE COUNT          688 a          297 c          274 g          738 t
ORIGIN      
        1 aatactattt actttatagt aaatacccct gaaaaccttt atttataagg cttacagaaa
       61 aaaagtttaa aaaaaaattg ctccaaaatt tggaaatata aaaatcattc ctatcttcac
      121 atcataaaat taattacacc aaaaaaataa aaaatgtaat tgatttttgt tatccattat
      181 cctaatattt tagatcaaat caaaaaccta gatgtctatg aaaactttaa ctcatgcgtc
      241 agatacgcaa ctaattcatg cgtttcaaga aagaaatgct aatgaagccc ttgaagtatt
      301 aattaatcgc tacaaggaca aaattttctc ttctatttta tttttagtaa aagacaagta
      361 tttagccgaa gacttatttc aagatgtgtt tattaaagta atagatacat taagaaacaa
      421 taagtattca gaagaaggta aatttttacc ttgggcttta cgcataagcc ataacttatg
      481 tgtagattac tttagaaaag taaaaagaac acctactatt aaaaccagcg atgatagaga
      541 tatttttgaa acaattaatg ttgtagaaga aagctctgaa acaaaattaa ttgccaacca
      601 aagtcatgat agagttcgta aaatgataga tatgctacct gatgaacaaa gagaggtttt
      661 agttttacgc cattttgcag acttaagctt taaagaaatt gcagatatta ccaattgcag
      721 tattaataca gcattaggta gaatgcgtta tggcttaatt aatttaagaa aactaatgtt
      781 agaaaagcaa attgctttat aaataataat agtaatcatt ataaaaaagt ttacagtttt
      841 atttggctgt aaactttttt tatggctata gtaatgctaa aaggcattaa gcaaccttag
      901 tttgcttttc ctcagtattg ctaagaatag aaaaaatttg attgatttta tttacagctt
      961 catctaattc ggttaacatt tgctttttat aagcttctga ctgattaaat atttgaggaa
     1021 tgatattctt ataataggta ataccatttt caagttgctc tttaaactta ttataatatt
     1081 taattttttt gtcgtttaaa tttttgatgc tatggttgat atctttttta atgtactcta
     1141 tatataaatg caattcatta atgtacatat ttgatctctt aacaccatct aataaattta
     1201 ttttaccata aatatgtttt accatatcgt ttaaactgta aattttatta aagtaagctg
     1261 tattggggcc agggcaaatt gctactgcag tgctttcatt tttctccaca atatcgttat
     1321 taatataagc tgcagttgcc aaaccttcgc ataggcaagc tttttcggta attatattaa
     1381 attgttcttg gtattcttct tggtttaaac cttgctcttg taattgctta atttttaaat
     1441 tttgatattg cctggatgct gtacaaattg gctctttagt aaactcggta ttagatacta
     1501 aaagtttttt aatgcatggg ctacctggtc ttcctttttc aattctttca attctttgtt
     1561 tctctgaact actgagttta aaattattaa aaggcactcc taaaggcgat gaaccactaa
     1621 tatagaaatc atctttagtt gctttggcta aattatttaa tgtagcctca tccacagtag
     1681 ttgcttctgg tactaataaa aatggactgc cccaaccagt gccatctaat tgataaaatt
     1741 gttgtaaaaa aatatcttct tcatgattgc ctatgccacc ttgataagta attttttgtg
     1801 tagggcaaac gttacaatta atatttttgg tttctaatgc tttttggtac atctcaaaaa
     1861 gttcgttaag cattgcattt ctattttgct taaactcttc taaaattggt cctgttaata
     1921 atccatctgt agcaaaagca tggccaccac agtttaaacc agattctact ctaaattcag
     1981 aaacccataa tcctttt
//
LOCUS       DAOQHP010000387         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_44390_length_1996_cov_3.057702, whole genome shotgun sequence.
ACCESSION   DAOQHP010000387 DAOQHP010000000
VERSION     DAOQHP010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44390_length_1996_cov_3.057702"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..921)
                     /locus_tag="PLY81_10110"
     CDS             complement(1..921)
                     /locus_tag="PLY81_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF30623.1"
                     /translation="MKKYLIIALCCLFVIMQACNNATTYNLNNDTPQTGKIFISVDES
                     FKPVIEQQIKVFQSSYPKANIIAEYKPEVECFKDLQEDSTRMILVARNINDKETEYFN
                     AKLSYTPKQDILAYDAVVVIVNINSKDSIFTLQQLQDILNGKNNITAVMDGSNATSTV
                     RFLKDSILKGEQFGKNVVASNGSNAVVEAIAKMPNAVGFVGLSWVGNTDEQNQKEQLN
                     KIKLALIECVKCNDKGVFAKPSQSTITYGQYPLARPLYYVLKEYSEGLGTGFKNFMTN
                     ERGQLIFRRAYLAPAKMNLNTRNSAIKEEQ"
     gene            complement(923..1153)
                     /locus_tag="PLY81_10115"
     CDS             complement(923..1153)
                     /locus_tag="PLY81_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30624.1"
                     /translation="MNSNEDKRRKSYLLMRMTYDIGMAVFLIGMAVFLLLGNFWGIEQ
                     ILTIDSTFRYMFGSICLLYGLFRLYRGIKREY"
     gene            1346..1804
                     /locus_tag="PLY81_10120"
     CDS             1346..1804
                     /locus_tag="PLY81_10120"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNF30625.1"
                     /translation="MKILMVCLGNICRSPLAEGILRTKANKLNLNWQVDSAGTADYHV
                     GKPPHILSQKVALENGIHIGAHKCRQFNKNDMLLFDKIYVMDTNNYLEVKEIAGKLWN
                     ENKVKLILSELYLNENKNVPDPWYGDEKDYYAVYELLDKACDAVITSLNK"
     gene            1811..>1996
                     /locus_tag="PLY81_10125"
     CDS             1811..>1996
                     /locus_tag="PLY81_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNF30626.1"
                     /translation="MSKISLPQGTRDFSADVVRKRNYIFNTIRTVFELYGFQPLETPA
                     MENIETLMGKYGDEGDKL"
BASE COUNT          707 a          312 c          278 g          699 t
ORIGIN      
        1 ttattgttct tcttttattg cactatttct tgtatttaaa ttcatttttg caggggctaa
       61 ataagctctt ctaaaaatta gctggcctct ttcgttggtc ataaaatttt taaaccctgt
      121 tccaagccct tctgaatatt cttttaatac atagtacaag ggtcgtgcca aagggtattg
      181 accataagta attgtgcttt ggcttggttt agcaaatact cctttatcat tgcattttac
      241 acattctatt aatgcaagtt taattttatt taactgttct ttttgatttt gctcatctgt
      301 attgccaacc caacttagcc caacaaagcc aacagcattg ggcattttag caattgcttc
      361 taccactgca ttactaccat tgcttgccac tacattttta ccaaattgtt ctccctttaa
      421 aatactatct tttaaaaacc ttacagtgct agtagcatta ctgccatcca ttactgcagt
      481 aatattattt tttccattta aaatatcttg gagttgttgc aatgtaaata tgctgtcttt
      541 actatttata tttacaatta ctaccacagc atcgtaagca agtatatctt gtttaggtgt
      601 gtaggatagt ttagcattaa aatattccgt ttctttatca ttaatatttc tagccactaa
      661 tatcattcta gtactatctt cctgcaaatc tttaaagcac tcaacttctg gcttatactc
      721 tgcaataata tttgctttag gatacgatga ttgaaaaact tttatttgtt gttcaataac
      781 aggcttaaaa ctttcatcta cactaataaa tattttacct gtttgtggtg tatcattatt
      841 taaattatat gttgttgcat tattacacgc ctgcataata acaaacaaac aacataaagc
      901 tattattaaa tactttttca tattaatact ctcttttaat acctctataa agtctgaata
      961 acccatacag taaacaaatg gagccaaaca tatatctaaa agtagaatca atagttaaga
     1021 tttgttcaat tccccaaaaa ttacccaata acaaaaaaac agccatgcca attaaaaaaa
     1081 cagccatgcc aatatcgtag gtcatacgca ttagcaaata actttttctt ctcttatcct
     1141 cgttgctatt cattactaat aaagtattaa gggctggaaa gatataaaaa taataattta
     1201 tgaaataaaa aaatactact atgtattaaa tactcctctg tttcaactat ttattacctt
     1261 aaaaagaggc aattaaaaat ataaatacta ttactaagga tgcttgatta gttaaagaat
     1321 aataacaaaa actatatttg ccctgatgaa aattttaatg gtttgtttag gaaatatttg
     1381 tagaagccct ttagcagaag gtatattaag aactaaagca aataaattaa accttaattg
     1441 gcaagtagat agtgcaggaa cagctgatta tcatgtaggg aaaccgcctc atattttatc
     1501 tcaaaaagtg gcactagaaa atggtattca cattggtgct cataaatgca ggcagtttaa
     1561 taaaaatgat atgcttttat ttgataagat ttatgtaatg gatactaata attacttaga
     1621 agtaaaagaa attgcaggta aattgtggaa tgaaaataaa gttaaattaa tactcagtga
     1681 actttatcta aatgaaaata aaaatgtacc agacccctgg tatggagatg aaaaagatta
     1741 ttatgctgta tatgagctat tagataaagc ctgcgatgca gttattacaa gtttaaacaa
     1801 gtaagtaatt atgagtaaaa tatcgttacc acaaggaacc agagatttta gtgctgatgt
     1861 tgtaaggaaa agaaattata tttttaatac tatacgtaca gtatttgagt tatatggttt
     1921 tcaaccatta gaaaccccag caatggaaaa tattgaaacc ttaatgggta aatatggtga
     1981 tgaaggagat aaactt
//
LOCUS       DAOQHP010000388         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_44722_length_1988_cov_3.713399, whole genome shotgun sequence.
ACCESSION   DAOQHP010000388 DAOQHP010000000
VERSION     DAOQHP010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44722_length_1988_cov_3.713399"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..827
                     /locus_tag="PLY81_10130"
     CDS             114..827
                     /locus_tag="PLY81_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30627.1"
                     /translation="MIIKNLTTTLFCTLLITIAVAQETTTIQLKHKVGDELLQLGTTS
                     YTNQWNEQFTVNKFKYYITALEVEFTNGNKNYSWHKKYHLVDEANAASKTIVLDKKLN
                     NIKAIHFLLGVDSIVNTNGIHTGDLDPMKTMFWTWNTGYIYAKLEGQSSSSKAQAHSF
                     SYHIGGYKANENAVKKISLLIKDNATTDNEIIIEANILAWFNGAKKLKIAEEPICHQP
                     GKLAMDIANNYEKMFSVKL"
     gene            833..1891
                     /locus_tag="PLY81_10135"
     CDS             833..1891
                     /locus_tag="PLY81_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNF30628.1"
                     /translation="MKTFKVITILICLVVVGVGFIEIKKHKPTPLIFIVPKGWPKPIY
                     NFKNNPLTVEGFELGKKLFYDERLSKDSTISCASCHQQFSAFATFDHNFSHGYNNTFT
                     TRNAPALQNLAWQKELMWDGGINHLDVQPITPIVAENEMAETLENVIYKLKKDTSYKR
                     MFKNAFGSNEITTERMTKALSQFMVMLVSSNSKYDKVKRGEDTFNLPEKLGYEIFKQK
                     CASCHTEPFFTDLSYRNTGMPLDATINDIGRMRITNNKNDSLKFKVPSLRNVDLTFPY
                     GHDGRFFTLSNVFEHYRSNMQPIHNTDILLKNKLYLSNFQIGQLTAFLFTLTDSSFTK
                     NPLFAAPAYTKPIILHQH"
BASE COUNT          795 a          278 c          305 g          610 t
ORIGIN      
        1 aaaagtagaa ttgctaccaa aacccctaat gctgttatag aacagaggaa ggaaaataaa
       61 ataataaagt ctaaacaaaa acaaaacaga aaaagaataa accttgatga attatgataa
      121 ttaaaaattt aaccactact cttttttgta ctttattaat tacaattgcc gtagcacaag
      181 aaacaacaac catacaatta aagcataaag taggagatga gttgttgcaa cttggtacta
      241 catcttacac aaatcaatgg aatgaacagt ttacagtaaa taagtttaaa tactacatta
      301 ctgccttaga agttgaattt acaaatggta acaaaaatta tagttggcat aagaaatatc
      361 atttggtaga tgaggccaat gctgcatcaa aaacaattgt attagataaa aagctcaaca
      421 atattaaagc aattcatttt ttgttagggg tagatagtat tgtaaatacg aatggcattc
      481 atactggcga tttagaccct atgaaaacaa tgttttggac atggaatact ggctatatat
      541 atgcaaaact agaagggcaa agtagttcct ctaaagcaca agcacatagc tttagttatc
      601 atattggtgg ttacaaagca aatgaaaatg cagttaaaaa aatatcactt ttaattaagg
      661 ataatgctac aactgataac gaaataatta tagaagctaa tattttggct tggtttaatg
      721 gggccaaaaa attaaaaatt gccgaagaac caatttgtca tcaaccaggt aaattagcaa
      781 tggatattgc aaataattat gaaaaaatgt tttcagtaaa attgtaactc aaatgaaaac
      841 ttttaaagta ataaccatac ttatttgttt agtagttgta ggagttggtt ttattgaaat
      901 taaaaaacat aaacctacac ctttaatatt tattgttccc aaaggttggc caaaaccaat
      961 ttataatttt aagaacaacc cattaactgt agaaggtttt gaattaggta aaaagctttt
     1021 ttacgatgaa agattaagca aagacagcac aattagttgt gcaagttgcc accagcaatt
     1081 tagtgctttt gccacttttg atcataattt tagccatggg tataacaaca cttttacaac
     1141 acgtaatgca ccagcattac aaaatttagc ttggcaaaaa gagttgatgt gggatggtgg
     1201 aataaatcat ttagatgtac agcctataac acccattgta gcagaaaatg aaatggcaga
     1261 aaccttagaa aatgtaattt ataaattaaa aaaagatacc agctacaaac gaatgtttaa
     1321 aaatgctttt ggtagcaatg aaattacaac agaaagaatg accaaagctt taagccaatt
     1381 tatggttatg ctagttagta gtaatagtaa atatgataaa gtaaaaagag gagaagatac
     1441 ttttaacctt cctgaaaaat taggctacga aatatttaaa caaaaatgtg ctagctgcca
     1501 taccgaacca ttttttacag atttaagtta tagaaacact ggaatgcctt tagatgctac
     1561 aattaacgat ataggaagaa tgagaataac caataataaa aacgattctt taaaatttaa
     1621 agttccctct ttacgcaatg tagatttaac ttttccgtat gggcatgatg gtaggttttt
     1681 tacattaagc aatgtgtttg agcattacag aagtaatatg cagcctatac ataatacaga
     1741 tatacttcta aaaaataaat tgtatttatc caactttcaa attgggcagt taactgcatt
     1801 tttatttaca ttaaccgata gcagttttac aaaaaatcct ctgtttgcag cccctgctta
     1861 tactaagcca ataattttac atcaacacta aagactaagt aattataggt aataatattt
     1921 taagaatttt ttatgaggta actttttaca ccataattaa ctacaaaatg aaaattcttt
     1981 ttattatt
//
LOCUS       DAOQHP010000389         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_44892_length_1984_cov_3.590461, whole genome shotgun sequence.
ACCESSION   DAOQHP010000389 DAOQHP010000000
VERSION     DAOQHP010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44892_length_1984_cov_3.590461"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..863)
                     /locus_tag="PLY81_10140"
     CDS             complement(96..863)
                     /locus_tag="PLY81_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF30629.1"
                     /translation="MSVTDKLKHIRIIRTCVYAVVGVITYPGLRIFNKLKIEGTENLK
                     NLPKKNVLFVSNHQTYFADVITFIHILSAVKWRKKNRLGLPYYLLNPFTNLYYVAAEE
                     TMKGSLISRFFILAGALTVKRTWRAEGKEVRRGLDPSDTRKINRALDSSWVITFPQGT
                     TKPFAPGRKGTGYIIKNNQPTVVPVVINGFWRAFNKKGLAFKKKGSILTVKFKEPMVI
                     DYTQPAEIILEQVMDAIEQSKKYMLKGKHHLMNVLDK"
     gene            997..1665
                     /gene="hxpB"
                     /locus_tag="PLY81_10145"
     CDS             997..1665
                     /gene="hxpB"
                     /locus_tag="PLY81_10145"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HNF30630.1"
                     /translation="MNFSTVIFDMDGLLIDSEPLWNKAASKVFNEYGVKLSNEQYNTT
                     TGLRTREFVQWWFKKFNIGDEELLKAETKIIDLIVQMVKENKGAIMPGVNYIIDFFQK
                     KNFKIGLATSSPMQLIDVVIELTNSKNYFQAIASAENLTYGKPHPQVYLDCAQQLNVH
                     PHECICFEDSFNGMIAAKAARMTCVVVPHHTQLKDERWGAADLKLTSLQNFGELHFGL
                     LSNK"
     gene            1743..>1984
                     /locus_tag="PLY81_10150"
     CDS             1743..>1984
                     /locus_tag="PLY81_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF30631.1"
                     /translation="MELSKNFEPQLVDNKWYSHWQTKGYFNSVVDATKQPYTIVIPPP
                     NVTGVLHMGHCLNNTIQDILIRHARMNGKNACWVPGT"
BASE COUNT          683 a          304 c          302 g          695 t
ORIGIN      
        1 tcttgaaaac cgttgactgt aacaggtccg ggggttcgaa tccctctctc tccgccaaca
       61 taaaaaaacc agagaaaatc tctggttttt ttatgttatt tatctaacac attcattaaa
      121 tgatgtttgc ctttaagcat atattttttg ctttgttcaa tagcatccat tacttgctct
      181 aaaattattt cagcaggttg tgtgtaatca attaccattg gctctttaaa tttaactgtt
      241 aatatggatc ctttcttttt aaatgctaat ccttttttat taaatgcacg ccaaaagcca
      301 ttaattacaa ctggtacaac agttggctga ttatttttaa ttatataacc agtacctttt
      361 ctgcctggtg caaaaggttt ggtagtacct tgcggaaagg tgattaccca acttgaatct
      421 aaagctctgt ttatttttct ggtatcacta gggtctaatc ctcttcttac ttctttgccc
      481 tctgcacgcc aggttctttt tacagttaat gcaccagcta aaataaaaaa tctacttatt
      541 aaactgcctt tcattgtttc ttctgctgcc acataataca aattagtgaa tgggttaagt
      601 aagtaataag gcaaacctaa tctatttttt tttcgccatt taactgccga tagaatatgt
      661 ataaaagtaa ttacatcggc aaaataagtt tgatgattac ttacaaaaag tacatttttt
      721 tttggtaaat tttttaaatt ttctgttcct tctattttta atttattgaa tattcttaaa
      781 ccaggatagg taataacacc aacaacagca taaacgcaag tacgaataat tctaatatgt
      841 ttaagtttat ctgtaacact cattacaatt aatttgagta taatattaaa tgcaatatag
      901 ctattaaatt tttattgaac ctaaagcagt aaataatttg ggtgtattta actatctata
      961 aaacattcaa atacatttgc aacaattata attgatatga attttagtac agtaattttt
     1021 gatatggatg gtttattaat tgatagtgaa ccattgtgga ataaagcagc tagtaaagtt
     1081 tttaatgaat atggtgttaa attaagtaat gaacaatata atactacaac aggtttaaga
     1141 accagagagt ttgtacaatg gtggtttaaa aaatttaata taggcgatga agaattatta
     1201 aaggccgaaa caaaaattat tgatttaata gtacaaatgg tcaaagaaaa taaaggtgca
     1261 ataatgccag gtgtaaatta tattatagat ttttttcaaa agaaaaactt taaaattggg
     1321 ttagccacat catcgccaat gcaattaata gatgtagtga tagaactaac caactctaaa
     1381 aactattttc aggcaattgc atctgccgaa aatttaacct acggaaaacc acacccacaa
     1441 gtttatttag attgtgccca acaattaaat gttcaccctc atgagtgcat ttgttttgaa
     1501 gattctttta atggaatgat agctgcaaaa gctgctagaa tgacttgtgt tgtggtgcca
     1561 catcatactc aattaaaaga tgaacgttgg ggagcagccg atttaaagtt aacttcttta
     1621 caaaatttcg gagaacttca ttttgggtta ttaagtaata aataataaac tctttattct
     1681 tttgctttta acttcatagc ctttgcttat ttttacagcc ttttataaaa taaggctaat
     1741 aaatggaatt aagtaaaaac tttgaacctc aacttgtaga taataaatgg tatagccatt
     1801 ggcaaacaaa aggctatttt aatagtgtgg tagatgccac caaacaacct tataccattg
     1861 ttattccacc acctaatgta acaggtgttt tacacatggg gcattgttta aataacacta
     1921 ttcaagatat attaattagg catgcaagaa tgaatggtaa aaatgcatgt tgggtgccag
     1981 gaac
//
LOCUS       DAOQHP010000390         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_45029_length_1981_cov_2.597092, whole genome shotgun sequence.
ACCESSION   DAOQHP010000390 DAOQHP010000000
VERSION     DAOQHP010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45029_length_1981_cov_2.597092"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PLY81_10155"
     CDS             <1..1883
                     /locus_tag="PLY81_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF30632.1"
                     /translation="LYKVAIAPATSIPKKSLKPNGPSIQENLGKVAPGRTYQDLIKSA
                     YDEKLFEFVTKAQLVINTNGIETPIGKPAIYKNIEASPNRAYLLLETINKPYSYLVPA
                     YGFATTVSVTDMKGNLVKEMAKLPSAETRPTGNDNVQNIPRGFTWRLDEAADIIWCEP
                     LDSGLIKNKAEFRDAVFGQAAPFKGEARELFKTKMRYRNTIWGNKNFAWVVEGLSGKQ
                     QTRVSNYSYLERKFETLFERNTTDAYNDPGTPVTTKNDYGKEVIYIKDNQIILNNTTG
                     SSVKGDLPYIALYNITNKQSNIIWRCDENNFEYVAEVLDKDRFTLITRKESTTEVPNY
                     YLRTVKGGSKLEALTNFVNPYKALEGIGFEKVRYVRADGIELTGNLYLPRGYVKNSGE
                     LLPLLIWAYPREFNNASDAGQIRGSKNKFTTISWASPLFYVTQGYAVLDNAEMPIVST
                     EENKKPNDNFVDQLRMNAEAAIDQIVNMGIADRNRIAIGGHSYGAFMTANLLAHTNLF
                     KAGIARSGAYNRTLTPFGFQNEDRTYWQAPNLYYEMSPFSFANKIKTPLLLIHGEADE
                     NPGTFPMNSERLFTAIKGHGGTVRLVMLPYEGHSYRSKENLLHMLWEQNEWLNKFVKE
                     AK"
BASE COUNT          740 a          274 c          370 g          597 t
ORIGIN      
        1 tgttgtataa agtagccata gcacctgcaa catctattcc taaaaaaagc ttaaagccta
       61 atggcccatc catacaagaa aacttaggaa aagttgcacc aggaagaacc tatcaagatt
      121 taattaaatc tgcttacgac gaaaaattat ttgaatttgt tactaaagct caattagtaa
      181 ttaataccaa tggtattgaa actccaattg gcaaacctgc catttataaa aacatagaag
      241 cttctcctaa cagggcatat ttacttttag aaacaattaa taaaccatat tcttatttag
      301 tacctgctta tggttttgct acaacggtaa gtgtaacaga catgaaagga aatttagtta
      361 aagaaatggc taaactacct tcagcagaaa ctaggccaac cggtaacgat aatgttcaaa
      421 atataccaag aggctttacc tggcgattag atgaagctgc tgatataatt tggtgcgagc
      481 ctttagatag tggcttaatt aaaaataaag ctgaatttag agatgctgtt tttggacaag
      541 ctgcaccttt taaaggagag gccagagaac tttttaaaac caaaatgagg tatagaaata
      601 ccatttgggg taacaaaaat tttgcttggg ttgtagaagg attaagtggt aaacaacaaa
      661 caagggttag taattatagt tatttagaaa gaaaatttga aacactattt gaaagaaata
      721 caactgatgc ttataatgac ccaggaacac ctgttacaac taaaaatgat tatggtaaag
      781 aagttattta tattaaagac aaccaaataa ttttaaataa tacaactggt tcttcggtta
      841 aaggagattt accatacatt gctttgtata atattactaa taaacaaagc aatattattt
      901 ggagatgcga tgaaaataat tttgagtatg tggcagaagt gttagataaa gatagattta
      961 ctttaattac aagaaaagaa tcaacaacag aggtgccaaa ttattattta agaacagtta
     1021 aaggaggtag taaacttgag gctttaacaa attttgttaa tccatataaa gcattagaag
     1081 gaattggctt tgaaaaagta agatatgtac gtgccgatgg tattgaactt acaggtaatt
     1141 tatacctgcc tagaggatat gtaaaaaact ctggtgagtt attaccatta ttaatttggg
     1201 cttatccaag agagtttaat aatgcttcag atgcaggcca aattagaggt agtaaaaata
     1261 aatttactac cattagttgg gcaagcccat tattttatgt aacgcagggt tatgctgttt
     1321 tagataatgc cgaaatgcca attgtaagca ctgaagaaaa taaaaaacca aatgacaatt
     1381 ttgtggatca attaaggatg aacgcagagg cagcaattga tcaaattgta aatatgggta
     1441 tagcagatag aaacaggata gctattggtg gccatagtta tggtgcattt atgactgcta
     1501 atttattggc tcatactaat ttatttaaag caggaattgc acgtagtgga gcatataata
     1561 gaacacttac tccttttggt tttcaaaatg aagacagaac ttactggcaa gcccctaatc
     1621 tttattatga aatgagccct tttagctttg caaataaaat taaaacacct ttattattaa
     1681 ttcatggcga ggcagatgaa aaccctggca cttttcctat gaatagtgaa aggttattta
     1741 ctgcaattaa aggtcatggt ggcacagttc gtttagtaat gttgccctac gaagggcata
     1801 gttatagaag taaagaaaat ttacttcaca tgttatggga gcaaaacgaa tggcttaata
     1861 aatttgtaaa agaagcaaaa taaattttaa ttagaagagg agtttagtag tggtaagata
     1921 atttttacgg tactatctta taaactgtgt aagtcttcat aaagttgggg taccccaact
     1981 t
//
LOCUS       DAOQHP010000391         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_45059_length_1980_cov_3.415584, whole genome shotgun sequence.
ACCESSION   DAOQHP010000391 DAOQHP010000000
VERSION     DAOQHP010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45059_length_1980_cov_3.415584"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="surE"
                     /locus_tag="PLY81_10160"
     CDS             complement(<1..493)
                     /gene="surE"
                     /locus_tag="PLY81_10160"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNF30633.1"
                     /translation="MAKITKKKEQPIILVTNDDSINAPGIKALVEAVKDLGRVVVVAP
                     DKPQSGMGHAITIGNPLRLNKVHFIDGVEAYTSNGTPVDCVKLAVDKILHRKPDICFS
                     GINHGANHSINVIYSGTMSAAMEAAIESIPSAGFSLLDFSLEADFTAAKTIVKKIAIQ
                     MLSK"
     gene            complement(546..1046)
                     /locus_tag="PLY81_10165"
     CDS             complement(546..1046)
                     /locus_tag="PLY81_10165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF30634.1"
                     /translation="MLQIRNAVENDIVAIQTIAKATWLKTYMQILSSEQMSYMLNWMY
                     SFEILKNQMQNNHAFFVGYLSQKQHEILGFAAISREAEKLFKLQKLYILPEQQKIGLG
                     KLLLQKVCNHAKENGANTLQLQVNRNNIAVNFYKKHGFKIIAEEDFDIGNGYFMNDYV
                     MELQFF"
     gene            complement(1048..1626)
                     /locus_tag="PLY81_10170"
     CDS             complement(1048..1626)
                     /locus_tag="PLY81_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF30635.1"
                     /translation="MLVLVCCIFNTSSLAQNLDIDILKEINPTNPNSNFWVNASASTY
                     PLATASTATLLAVSYLSKNKNLQNKGWKLAGSLLINTAITQSLKTVINRERPYEKYPT
                     LITPYEYKIGHSFPSGHTSVAFATATSLTISCKKWYVVVPAFAWASSVAFSRLYLGEH
                     YPTDIVVGALIGSGSAVISNWLTNKILINKKK"
     gene            complement(1667..>1980)
                     /locus_tag="PLY81_10175"
     CDS             complement(1667..>1980)
                     /locus_tag="PLY81_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30636.1"
                     /translation="VAPVGINILTGAKGHFLETGFNISFVSAKNKNKTTPKPSLFGQI
                     DFSRNQTYLVYGVPVGYRWHKTDKPGVSFRAGVEPLIGKRIDDKFVFALTGHLSIGYS
                     F"
BASE COUNT          632 a          336 c          269 g          743 t
ORIGIN      
        1 ttttagaaag catttgtata gcaatttttt taacaatagt ttttgcagca gtaaaatctg
       61 cttctaaact aaaatctaat aagctaaaac ctgcacttgg tatgctttct attgcagctt
      121 ccatagcagc actcatagta ccactataaa taacattaat gctgtggttt gcaccatgat
      181 ttatgccact aaaacaaata tctggtttac gatgtaaaat tttatctact gcaagtttca
      241 cacaatctac aggtgtacca ttgctggtat atgcttctac cccatctata aaatgtactt
      301 tgtttaatcg taaagggtta ccaattgtta tagcatgtcc cataccactt tgtggtttat
      361 ctggagctac aaccactacc ctgcctaaat cttttacagc ttctaccaag gcttttatac
      421 ctggtgcatt tatgctatca tcattagtaa ctaatattat gggttgttcc ttcttctttg
      481 taatttttgc catgagtgca atataataaa gagttgattg taataattat ttttcactat
      541 ttgaattaaa agaattgcaa ttccataaca taatcattca taaaatagcc attgcctata
      601 tcaaagtctt cttcagcaat aattttaaat ccatgttttt tataaaagtt tactgcaata
      661 ttatttctgt ttacttgtaa ttgtaatgta ttagcaccat tttcttttgc atggttacac
      721 actttttgta gcaatagttt tcctaaacca attttttgct gttctggcaa aatgtataat
      781 ttttgaagtt taaataattt ttctgcctct ctagaaatag ctgcaaaacc tagtatttca
      841 tgttgttttt gacttaaata accaacaaaa aaagcatggt tattttgcat ttgatttttg
      901 aggatttcaa aactatacat ccagtttagc atatagctca tctgctctga acttaatatt
      961 tgcatatatg ttttaagcca tgtagctttt gcaattgttt gaattgccac aatatcattt
     1021 tctactgcat ttcttatttg cagcatatta ttttttttta tttattaaaa ttttgtttgt
     1081 tagccaattg ctgattactg ctgagccaga gccaattaaa gcacctacta caatatctgt
     1141 tggataatgc tctcctaaat acaatcttga aaaagcaaca ctactagccc atgcaaatgc
     1201 aggtactaca acataccatt ttttacaaga aatggttaaa gatgttgcag ttgcaaaagc
     1261 tacagaagta tgtccagatg gaaatgaatg gccaatttta tattcgtaag gggtaattaa
     1321 tgttgggtac ttttcataag gcctttctct attaataact gtttttaatg attgagttat
     1381 agccgtattt ataagtaaac tgccagcaag tttccatcct ttattttgta aatttttatt
     1441 tttacttaaa taacttactg ctaacaatgt agcagtagaa gctgttgcta atggataagt
     1501 tgaagcagag gcattaaccc aaaaatttga attgggattt gttggattta tttcttttaa
     1561 gatatcaata tctaagtttt gtgctaaaga agaagtatta aatatgcaac atacaagaac
     1621 aagcatcaaa attttattac aataaatttt tagcatcttt ctattattaa aaactgtacc
     1681 caatacttag atgtccagtt aatgcaaata caaatttatc atctattctt ttacctatca
     1741 atggttcaac accagctcta aaacttaccc ctggcttatc tgttttatgc caacgataac
     1801 caactggcac accatacact aagtatgttt gatttctgga aaaatctatt tgcccaaaca
     1861 aagaaggctt tggtgttgtt ttatttttat tttttgcact cacaaaactt atattaaaac
     1921 cagtttctaa aaaatgtcct ttagctcctg ttaaaatatt tatacctaca ggtgcaacaa
//
LOCUS       DAOQHP010000392         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_45321_length_1974_cov_3.592496, whole genome shotgun sequence.
ACCESSION   DAOQHP010000392 DAOQHP010000000
VERSION     DAOQHP010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45321_length_1974_cov_3.592496"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLY81_10180"
     CDS             complement(<1..329)
                     /locus_tag="PLY81_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF30637.1"
                     /translation="MTIQDIINVLENYAPPALQESYDNVGLLIGNKNWNCTGIICCLD
                     STEDVIKEAISKKCNLIVAHHPIIFSGLKKLNGSNYVEQTIITAIKNDIAIYAIHTNL
                     DNLIDGV"
     gene            complement(469..1794)
                     /gene="ahcY"
                     /locus_tag="PLY81_10185"
     CDS             complement(469..1794)
                     /gene="ahcY"
                     /locus_tag="PLY81_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNF30638.1"
                     /translation="MSTLTKTIDFTLPYKVADISLAEWGRKEIRLAEAEMPGLMSIRA
                     EYGASQPLKGARIAGCLHMTIQTAVLIETLVALGAEVKWSSCNIFSTQDHAAAAIAAA
                     GIGVYAWKGQTQEEADWCIEQTLFFGGADRPLNMILDDGGDLTNMVFDKYPEFVSGIK
                     GLSEETTTGVHRLYERMVKGTLPIPAINVNDSVTKSKFDNKYGCKESLVDSIRRATDV
                     MMAGKVAVVGSYGDVGKGSAASLKGAGCRVIVTEIDPICALQAAMDGFEVKPMIKAVA
                     EADIIVTASGCRDLITEKHFRLMKDKAIVCNIGHFDIEIDMAWLNDNFGHTKNTIKPQ
                     VDLYTIDGKDVIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWTNTDKYENKV
                     YVLPKHLDEKVARLHLAKIGVELDVLSDEQAAYLGIPKNGPFKAEHYRY"
BASE COUNT          681 a          360 c          256 g          677 t
ORIGIN      
        1 tttacgccat ctattaagtt atctaaattg gtatgaattg cataaatggc aatatcattt
       61 ttaatagctg taataattgt ttgttcaaca taattactac cattaagctt ttttagacca
      121 ctaaaaatta ttggatgatg tgcaactatt aaattgcatt ttttggatat tgcttctttt
      181 ataacatctt ctgtagaatc aaggcaacaa ataattcctg tacaattcca atttttattt
      241 cctattaata atccaacatt atcataactc tcttgtaatg ctggtggagc ataattctct
      301 aaaacattaa taatatcttg tatagtcatt tgctacaaag ctataagaag taatttaaaa
      361 atataaagta ttggcttatt tattcaataa taccaccacc ctattaataa tataacaaaa
      421 aaggtacaat tacattgtac ctttaatagt aagtgtatgt gagaaagatt aatacctgta
      481 atgctctgct ttaaaagggc catttttagg aatacctaaa taagcagctt gttcatcaga
      541 taatacatca agttcaacac caatttttgc aaggtgcaaa cgtgctactt tttcatcaag
      601 atgctttggt aaaacataaa ctttgttttc atatttatca gtatttgtcc aaagttctat
      661 ctgagctaaa gtttggttgg taaatgaatt actcattaca aaacttgggt gtcctgttgc
      721 acaacctaaa tttactaaac gaccttcggc taaaactatt acatctttac catcaatagt
      781 gtaaagatct acttgaggtt taatggtatt ctttgtatga ccaaaattgt catttaacca
      841 agccatatca atttcaatat caaagtgccc aatgttacaa acaatagctt tgtctttcat
      901 taatctaaaa tgtttttcag taattaaatc acgacaacca gaagcagtaa ctataatatc
      961 agcttctgct acagctttaa tcattggttt tacttcaaag ccatccatgg cagcttgtaa
     1021 agcacaaatt gggtctattt cagtaacaat tacacgacaa ccagcaccct ttaatgaagc
     1081 agcagaacct ttacctacat ctccataaga accaacaaca gcaacctttc cagccatcat
     1141 aacatctgta gctctacgaa tagagtcaac taaagattct ttacagccat atttgttatc
     1201 aaatttagat ttagtaacac tgtcgtttac attaattgca ggaattggta atgtgccttt
     1261 taccattctt tcatataaac gatgtacacc tgttgtagtt tcttcactca aaccttttat
     1321 gcctgaaaca aattcagggt atttatcaaa taccatattt gttaaatcgc ctccatcatc
     1381 taatatcata tttaatggtc tatctgcacc accaaaaaat aatgtttgtt ctatacacca
     1441 atcagcttct tcttgagttt gacctttcca agcatacact ccaatgccag cagcagcaat
     1501 agcagcagca gcatgatctt gtgtagaaaa aatgttacaa gaactccatt taacttctgc
     1561 acctaaagca actaatgttt caattaaaac tgcagtttga atggtcatgt gtaaacaacc
     1621 tgcaattctt gcacctttta atggttgaga tgctccgtac tcagcacgaa tactcattaa
     1681 acctggcatt tctgcttcgg ctaaacgaat ttcttttctc ccccactctg caagactaat
     1741 atctgccact ttgtatggca atgtaaaatc aattgttttt gtaagcgttg acatatttta
     1801 tttttattag aacgcaaaag tatttaataa agaataattt tttgcaaact attaataatt
     1861 tagaatattt tattaatttt atagtaatat ttagaacaaa taataagtaa cttatggcta
     1921 aaacagtaaa aaaagaacaa accactaatg aaagcattgt attagataat aaag
//
LOCUS       DAOQHP010000393         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_45775_length_1963_cov_3.457547, whole genome shotgun sequence.
ACCESSION   DAOQHP010000393 DAOQHP010000000
VERSION     DAOQHP010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45775_length_1963_cov_3.457547"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLY81_10190"
     CDS             <1..321
                     /locus_tag="PLY81_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HNF30639.1"
                     /translation="IVAVGCNDESLAKAINLVIKEQGGISAVSNNNEKVVGLPIAGLM
                     SAEDGYKVANAYTDIDLMTKKELGSTLAAPFMTLSFMALLVIPYLKLSDKGLFDGEKF
                     EFVN"
     gene            329..916
                     /locus_tag="PLY81_10195"
     CDS             329..916
                     /locus_tag="PLY81_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNF30640.1"
                     /translation="MIIIDNKLISDEIVEEQFVCDLEKCKGGCCEDGDAGAPLEDWEK
                     KEVEKFYSSVLPYLTQDGIEEINKQGLFVYDKTFGWVTPTINGGICVYAKKDHQNIIH
                     CAFEQAYNDGKISWKKPLSCHLFPIKTSISKEDPDLEYINYEPREDLCKAACSLGKKL
                     KIPAYIFLKEALIRKYGEEFYNALEASANHLKSNK"
     gene            1136..>1963
                     /locus_tag="PLY81_10200"
     CDS             1136..>1963
                     /locus_tag="PLY81_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNF30641.1"
                     /translation="MLIKGIPISLSLNNSINDTEADEKNKYQKKRLIKLSLLAIAVAM
                     VVSGFAKVLVHLIDLITNIAFYQNFSFIPSSPANNSIGWWVVLIPVVGGLLVGIMALY
                     GSKAIRGHGIPEAMEQILTNQSKIKPSITFLKPISAAISIGTGGPFGAEGPIIATGGA
                     LGSTLGQILKITPNERKILLASGATAGMSAIFGSPIAAIFLAIELLLFEFSPRSFIPV
                     ALACITGAAGHHFLFSSSVVFPMLQTILPASNKALLFYSIMGVAVGILSVIATKIVYW
                     "
BASE COUNT          684 a          266 c          345 g          668 t
ORIGIN      
        1 attgtggctg ttggttgtaa cgatgaaagc cttgctaaag caattaattt agttataaaa
       61 gaacaaggag gtattagtgc tgtaagcaac aataacgaaa aagtggttgg cttgccaatt
      121 gcaggtttaa tgagtgcaga agatggttat aaagttgcca atgcttatac agatatagat
      181 ttgatgacta aaaaagaatt aggtagtaca ttagcagctc catttatgac tttgagtttt
      241 atggctctat tggttattcc ttatttaaaa ctgagtgata aaggattatt tgatggagaa
      301 aaatttgaat ttgtgaatta attattttat gataattata gataataaac taattagcga
      361 tgaaattgtt gaagaacaat ttgtttgtga tttagaaaaa tgtaaaggtg gttgctgcga
      421 agatggagat gcaggtgcac cattagaaga ttgggaaaag aaagaggttg aaaaatttta
      481 ctcctctgtt ttaccatact taacacaaga tggaattgaa gaaataaata aacaaggttt
      541 atttgtttat gataaaactt ttggctgggt aacaccaacc attaatggag gtatttgtgt
      601 atatgcaaaa aaagatcatc aaaatattat tcattgtgca tttgagcaag cctataacga
      661 tggaaaaata agttggaaaa agcctttaag ttgccattta tttcccatta aaacatctat
      721 aagcaaagaa gaccctgatt tagaatatat taattacgag ccaagagaag atttatgtaa
      781 agcagcttgc agccttggta aaaaattaaa aataccagca tatatttttt taaaagaagc
      841 cttaataaga aaatatggag aagagtttta taatgcatta gaagcaagtg ctaatcattt
      901 aaagagtaat aagtaaccta ctctgtttaa ttcatcttaa ctaaaaataa gtattaaata
      961 agtttgcatt tttattataa aaaacaattt actctttaat gtattagtta gaagttattt
     1021 tttatcatca acataaataa cctctttatg gcattataat tgcataaatt tgcaaaccta
     1081 atcaacactc tcaaaccctt tcttaacaat ttattgttta cttaaaccaa catgaatgtt
     1141 aattaaagga attcctattt ctctatctct taataactct atcaacgata cagaagcaga
     1201 tgaaaaaaat aaataccaga aaaaaagatt aataaaactt tctttactag ctatagctgt
     1261 tgcaatggtt gttagtggtt ttgcaaaagt tttagtacac ttaatagatt taataaccaa
     1321 tattgctttc tatcaaaatt ttagtttcat accatcaagt cctgcaaata actctattgg
     1381 ttggtgggtt gtgttaattc ctgtagttgg aggtttattg gtaggcataa tggccttata
     1441 tggttccaaa gctatacgtg gccatggtat tccagaagca atggaacaaa tactaactaa
     1501 ccaaagtaaa ataaaacctt ctattacttt tttaaaaccc atttctgctg caatatctat
     1561 tggcactggt ggaccatttg gtgctgaggg cccaataatt gcaacaggtg gtgcattagg
     1621 ctctacactt ggtcaaattt taaaaattac accaaacgaa agaaaaattt tactagcatc
     1681 tggtgcaaca gcaggtatgt cggcaatttt tggtagccca atagcagcaa tttttttagc
     1741 aatagaattg ttgttgttcg agttttctcc tcgttctttt attccggttg cattagcttg
     1801 tattactggt gctgcagggc atcatttctt gtttagcagt agtgtagttt ttccaatgtt
     1861 gcaaactatt ttacctgcca gcaataaggc attattattt tatagtatta tgggtgttgc
     1921 tgttggtata ttatctgtaa tagcaaccaa aatagtgtat tgg
//
LOCUS       DAOQHP010000394         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_45862_length_1961_cov_4.380902, whole genome shotgun sequence.
ACCESSION   DAOQHP010000394 DAOQHP010000000
VERSION     DAOQHP010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45862_length_1961_cov_4.380902"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLY81_10205"
     CDS             complement(<1..267)
                     /locus_tag="PLY81_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30642.1"
                     /translation="MFKKVITFCVLSIFSFQVKAQVMDAFVHEGEFGIGVGAGHYFGD
                     LTAGMKLIDIKPKLSATIFFRKQFNNYIGIKIAGTFAFLGYADKY"
     gene            complement(371..1249)
                     /locus_tag="PLY81_10210"
     CDS             complement(371..1249)
                     /locus_tag="PLY81_10210"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNF30643.1"
                     /translation="MKIAIYSRGLDASLENPLVYLLNELVKYNIEIVVYRQLPQLYHL
                     PNELKSIISTFQFSEELMPDIDCLISLGGDGTMLDAVALVKDKNIPILGVNIGRLGFL
                     ANINKDELSLAVDAIINRTYTIDKRSLIHVDANVGLFNDAPFALNEFAILKRDTSPMI
                     KIHTYLNGEFLNTFWADGLLVSTPTGSTGYNMSCNGPIVFPDASNFIITPIAPHNLNV
                     RSIVVSDSNIISFEIEGRAEQFICALDARREIVNKEVQLAVKKEDFYINIIRLNEGSF
                     LSTLRNKLTWGMDKRN"
     gene            1474..>1961
                     /locus_tag="PLY81_10215"
     CDS             1474..>1961
                     /locus_tag="PLY81_10215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNF30644.1"
                     /translation="MNTTFNPDKQHSIATSVSISGTGLHTGILADMTLLPANPGFGIQ
                     FQRTDLPNKPIIKADCDYVTDTSRGTTLQSGDAKVNTVEHILAALVGMGVDNCLIEIN
                     GPEIPIMDGSSKDFVDIISTVGVVEQDAAKAWYSIDENIIYYDESKRVEMVAMPAMDY
                     QIT"
BASE COUNT          713 a          295 c          269 g          684 t
ORIGIN      
        1 gtatttatct gcatagccta aaaaagcaaa agttccagct atttttattc ctatataatt
       61 attaaattgt ttacgaaaaa aaatagttgc cgatagttta ggtttaatat caattaattt
      121 cataccagca gttaagtcgc caaaataatg acctgctcca acaccaattc caaattcacc
      181 ttcatgtaca aaggcatcca taacttgtgc ctttacctga aatgaaaaaa tacttaaaac
      241 acaaaaagta ataacttttt taaacatagg ggcaaattaa cgtactaata agggttaaaa
      301 cgaaaattag atatattttc ttataattat tgagttaaaa ttttaataca aaacataaaa
      361 aatattgggt ttagtttctt ttatccattc cccaggttag cttatttctt aaagtagata
      421 aaaaactacc ttcatttaaa cgaataatat ttatataaaa atcttctttt tttactgcta
      481 attgtacctc tttatttaca atttctcttc tggcatctaa tgcacaaata aattgttctg
      541 ccctaccttc tatttcaaaa gaaataatat tactatcaga tacaacaata cttctaacat
      601 ttaagttatg gggtgcaata ggtgttataa taaaatttga tgcatcggga aatacaattg
      661 gcccattaca actcatatta taccctgtac tacctgttgg ggtagaaact aacaaaccat
      721 ctgcccaaaa agtatttaaa aattctccat tcaagtaagt atgaatttta atcattggtg
      781 aagtatctct ttttagtata gcaaattcat ttaatgcaaa tggggcatcg ttaaacaaac
      841 caacattagc atctacatgt ataagagatc gtttatcaat agtataagtt ctatttataa
      901 ttgcatctac agccaaagaa agttcatctt tattaatatt tgctaaaaag cctagcctgc
      961 caatatttac acctaaaatt ggaatatttt tatcttttac taaagcaact gcatccagca
     1021 tagttccatc tccaccaagg cttattaaac aatctatatc tggcattaat tcttctgaaa
     1081 attgaaaagt agaaataata ctttttaatt cgttaggtaa atgatataat tgtggcaatt
     1141 gcctataaac tacaatttca atattatatt ttaccaattc atttaataaa taaactagtg
     1201 gattttctaa acttgcatct aaccctctac tataaatagc tattttcatt caagtttttt
     1261 aaatggtgta gcatttttta ttgaagcggt aaagatactt aataacctta atgcaataaa
     1321 tatattattt tggataaaat ttataaaatc aatgtaaaat attgaacttt aatttgttca
     1381 ctaacactta tttttgccct ttatttaatg tagggttaaa aaattatatt ttgatatata
     1441 ttacttttat ttcctgcaat taataaacta tttatgaata ctacttttaa tcctgataag
     1501 caacatagta ttgcaaccag tgtaagtatt agtggaacag gtttacatac tggaatttta
     1561 gcagatatga ctttattgcc agctaaccca ggttttggta ttcaatttca aagaacagat
     1621 ttgcctaata agcctataat taaggcagat tgtgattatg taacggatac atctagaggt
     1681 actactttac aaagtggcga tgcaaaagtt aatactgtag agcatatttt agctgcattg
     1741 gtaggtatgg gtgtggataa ttgcttaata gaaataaacg gaccagaaat accaataatg
     1801 gacgggagca gcaaagactt tgtagatatt atttctacag ttggtgtggt tgaacaagat
     1861 gctgcaaaag catggtatag tattgatgaa aatattattt actacgatga gtctaaaaga
     1921 gtagaaatgg tagctatgcc tgcaatggac tatcagataa c
//
LOCUS       DAOQHP010000395         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_46331_length_1949_cov_5.776663, whole genome shotgun sequence.
ACCESSION   DAOQHP010000395 DAOQHP010000000
VERSION     DAOQHP010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46331_length_1949_cov_5.776663"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="PLY81_10220"
     CDS             complement(<1..1835)
                     /locus_tag="PLY81_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30645.1"
                     /translation="MNIKNIGRNDPCPCGSGKKFKQCCQNKQENQSETAKTRQLENIP
                     TLFAKARQASLQNDFITAEKIYNEILTINSKHILTLNNLGILKQNQGDHTAAITFFKR
                     ALKIEPNAATHSNLSTSLIALELKDEAIQHLKTAISMNPGDYIAHTNLGIIYCSRNNY
                     KDGYIHLNKSLQINPNYPVSLYNLAAFLMRQGKYKKAEEYFKRTLDLAPNYLTYQHYL
                     FCLCFDEKAFPYKYLELAKNFENFYLNKRSFMFDKWPNSSDVNASQLKIGFVSGDLRN
                     HPVGFFIENIIYALNKNKTMLFAYSNSTLETELTLRIKPNFNKWYIVKSLTDEQLAQQ
                     IYNDGIDILIDLSGYTAHNRLNMFAYKPAPIQVSWLGFFASTGLSFIDYFIADPIAIP
                     KDKQFYFSEKIYYMPETRLCFTPPNDDIATPNDLPLIRNQYITFGCFQNLSKINDITL
                     PLWSKILKATEGKLLIKSPQLDDPLIKLEFLEYLESFNILEDKIILQGHSSREDYLNS
                     YHNVDFMLDTFPYPGGTTTCEALWMGVPTLTLTGNTLLERQGHSMLYNAGLENWVCTT
                     AEQYIQKAIDFSKQIDYLSNLRQNLRHQVKLSPLMNSERFALNFE"
BASE COUNT          606 a          271 c          331 g          741 t
ORIGIN      
        1 ttttcaaagt ttaatgcaaa tcgttcagaa ttcattaaag gtgagagttt aacttgatga
       61 cgcaagtttt ggcgaagatt ggaaagatag tcaatttgct tactgaaatc gattgctttt
      121 tgtatgtatt gctcagcagt tgtgcaaacc caattttcta agccagcatt atataacata
      181 ctatggcctt gacgctctaa taatgtattt cctgttaatg ttagtgttgg tactcccatc
      241 cataatgctt cgcaagtagt cgttccacca ggatatggaa aagtatcaag cataaaatct
      301 acattatgat agctattgag gtaatcttca cggctagagt gaccctgtaa tataatttta
      361 tcttctaaga tattaaaaga ttcaagatat tctaaaaact ctagtttaat caatgggtca
      421 tctaattggg gactttttat taataatttc ccttctgtgg ctttgagaat tttactccac
      481 aaaggcaaag tgatatcatt aattttgctt aaattttgaa agcatccaaa ggtaatatat
      541 tgattacgaa ttagtggtaa atcatttggg gttgcgatat cgtcatttgg tggtgtaaag
      601 cataaacgtg tttcaggcat ataatatatt ttttcgctga agtaaaattg tttgtctttg
      661 ggaatggcga tagggtctgc tataaaataa tcaataaagc ttaaaccagt tgaggcaaaa
      721 aaacctaacc agcttacttg aataggtgca ggtttataag caaacatatt taagcggtta
      781 tgtgcagtat aaccacttag atcaattaag atatcaattc catcattata aatttgttgt
      841 gctaattgct cgtctgttaa agatttaact atataccatt tattaaaatt aggtttaata
      901 cgaagcgtta attcggtttc taatgtgcta ttgctatatg caaataacat ggttttattt
      961 ttgtttaagg catatattat attttcaata aaaaatccta ctgggtggtt acgtaaatca
     1021 ccagaaacaa aacctatttt taattggctg gcatttacat ctgagctatt tggccattta
     1081 tcaaacataa aactacgttt attcaggtaa aaattttcaa aatttttggc gagctctaaa
     1141 tatttatatg gaaatgcttt ctcgtcaaaa cataaacaaa ataggtaatg ttgatatgtt
     1201 aaatagtttg gagctaaatc gagtgttctt ttgaagtact cttcagcttt tttatattta
     1261 ccttgtcgca ttaaaaatgc agctaaatta tataagctaa ctggatagtt agggtttatt
     1321 tgtagggatt tatttaaatg aatataacca tctttatagt tatttctact acaataaata
     1381 atacctaaat tggtatgtgc aatataatca ccggggttca ttgaaatcgc agttttaaga
     1441 tgttgtattg cttcatcttt aagctctaat gctatgagtg atgtacttaa atttgagtgg
     1501 gttgctgcat taggttctat ttttaaagcg cgtttaaaaa aagtgatagc ggcagtatga
     1561 tcaccttgat tttgttttag tataccaaga ttgtttaatg ttagaatgtg tttggagttg
     1621 atcgtcaaaa tttcattata aattttttct gctgttataa agtcattttg caaagacgct
     1681 tgtctagctt tagcaaataa agttggaata ttttcaagtt gtcttgtttt tgcagtttca
     1741 ctctgatttt cttgtttatt ttgacagcat tgcttaaatt ttttgccact tccacaagga
     1801 caagggtcat ttctgcctat atttttaatg ttcataaata attacctcaa tgactttatt
     1861 ttttatggtt ttgccatata tttactagtg tttgctcaaa ttcatttgca aattttttgg
     1921 cattcataat gggcatttct agaagtgtt
//
LOCUS       DAOQHP010000396         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_46433_length_1947_cov_4.469345, whole genome shotgun sequence.
ACCESSION   DAOQHP010000396 DAOQHP010000000
VERSION     DAOQHP010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46433_length_1947_cov_4.469345"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1419)
                     /locus_tag="PLY81_10225"
     CDS             complement(316..1419)
                     /locus_tag="PLY81_10225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF30646.1"
                     /translation="MKKLSLILLASTVIFSCKTKENGAFTVSGNINNASTDKVYLEEM
                     PFAAEKSIVIDSATIKDGKFELNGLANTEGLYALTILNGPRVLLVNDSKDIDVNLDVH
                     QYKNYQIKGSKATISLKNLFTDYEAQFSKVKRAYTILDSLKELVTSDSILTIHKLSKE
                     QEMGKLNKILSNFVTSTESPAACLQALVMATRSMPLEELQTLALNAASKFKDYDGMQK
                     FNTMLRGNQYPLLNQQAPEFTLNTPDNKPIALSSFKGKYVLVDFWASWCKPCREENPT
                     VVAAYNKYKDKNFTVFGVSLDSDKNSWVEAIKKDNLTWLHVSDLLQWETPMVGLYKFD
                     GIPFNVLIDPSGKIIATGLRGDALDKKLSEILN"
     gene            complement(1455..>1947)
                     /gene="gatB"
                     /locus_tag="PLY81_10230"
     CDS             complement(1455..>1947)
                     /gene="gatB"
                     /locus_tag="PLY81_10230"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613106.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNF30647.1"
                     /translation="YDADQLISDKSTATYFLNTTKFSTNHKAIANWINGPLKQHVNTK
                     EDSFENLKLSPKKLALLIEIVENGTINFSTASTKILPVLLAEDKQPMQIAEELNLVQK
                     NNTTEIEQWISIVLSNMPDKVEEYKKGKKGLLGLFVGEVKKLSKGKADPKIATQLLTE
                     KLQ"
BASE COUNT          596 a          307 c          285 g          759 t
ORIGIN      
        1 acaacagcag aacccttacg agactatagg gcatccgcta acaagggttt gccaaaatag
       61 tgacagaagt gctgaatgga gcattagtac ttctattcaa cattagtact aaattcaacg
      121 gctgtgtaat tatatttaac tttattgtaa aataggatta atcaaaatca tgtttgaaga
      181 atgttaccta gattattttt ttaacctcaa taaaatagta aaaaaatctc agaattttct
      241 tactctgaaa atatgtaaga aaactctgag atagcactag caaaagttta aatattgtta
      301 gtgaaagttt tattactagt ttaaaatttc acttaatttt ttatctaaag catctcctct
      361 taatccggta gcaataattt ttcctgaagg gtctattaat acattaaatg gtattccatc
      421 aaatttatat aagcctacca ttggtgtctc ccattgcagt aaatcactta catgtaacca
      481 tgttagatta tcttttttta ttgcttctac ccaactgttt ttatcgctgt ctaacgatac
      541 accaaatact gtaaagtttt tgtctttgta tttattatag gctgctacaa ctgttgggtt
      601 ttcttctcta catggtttac accaacttgc ccaaaagtca accaatacat attttccttt
      661 aaaactactt aaagcaattg gcttattatc tggtgtattt aaagtaaatt caggtgcttg
      721 ttggttaagt aaaggatatt ggttacctct taacatggta ttaaattttt gcataccatc
      781 ataatcttta aatttagaag ctgcatttaa ggcaagtgtt tgcaattctt ctaaaggcat
      841 actacgggtt gccattacca aagcttgtaa gcaagcagcg gggctttcgg ttgatgtaac
      901 aaaattggat aaaattttat ttaacttacc catctcttgc tcttttgata acttatgtat
      961 agttaatata ctatcagaag taactaattc ttttaacgaa tctaaaatag tgtatgctct
     1021 ttttacttta gaaaattgtg cttcgtaatc agtaaataaa ttttttaaag aaatggtagc
     1081 tttagagcct tttatttgat aatttttata ctgatgaacg tctaagttta catcaatatc
     1141 tttactgtca tttacaagta aaacccttgg cccgtttaaa attgttaaag catataatcc
     1201 ttcggtattt gctaacccat taagctcaaa tttaccatct ttaatagtgg cagagtctat
     1261 tacaattgat ttttcagcag caaagggcat ttcttctaaa tacactttat ctgtactggc
     1321 attattaata tttcccgaaa ctgtgaaagc tccattttct tttgttttgc aagaaaaaat
     1381 tactgtggag gctaataaaa ttaaacttag ttttttcatt aatctactta tttgggttaa
     1441 aatttataac tattttattg tagtttttca gttaataatt gtgttgcaat tttagggtct
     1501 gctttaccct tgctcagctt ttttacttct ccaacaaata atcctaaaag ccctttttta
     1561 ccttttttat attcttcaac tttatcgggc atattggaaa gtacaatgct tatccattgc
     1621 tcaatttctg tggtattatt tttttgcact aaatttaatt cttctgcaat ctgcattggt
     1681 tgcttgtctt cggctagtaa aactggtaat attttagtag atgcagtgga aaaatttatt
     1741 gtaccatttt ctacaatctc tattaataaa gcaagttttt tagggcttaa tttaagattt
     1801 tcaaaactgt cttccttggt gttaacatgc tgttttagtg gcccatttat ccagtttgca
     1861 attgctttat ggttagtaga aaattttgtt gtgtttaaaa aataggttgc tgtgctttta
     1921 tctgagatta gttgatcagc atcgtat
//
LOCUS       DAOQHP010000397         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_46794_length_1939_cov_3.287155, whole genome shotgun sequence.
ACCESSION   DAOQHP010000397 DAOQHP010000000
VERSION     DAOQHP010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46794_length_1939_cov_3.287155"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1151)
                     /gene="prfB"
                     /locus_tag="PLY81_10235"
     CDS             complement(join(59..1102,1104..1151))
                     /gene="prfB"
                     /locus_tag="PLY81_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793088.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNF30648.1"
                     /translation="MTHEKLNDIKERLLVLGGFFDYDNRIAKIEQDKQISLSAGFWDD
                     NKRATSIMKEIKVNEYWVKLFNTVETAIDDFAVLFEFYKAADATEEETLNAFNNAISE
                     LDNAEFKSTLNKPEDELNAVLQINSGAGGTESQDWAEMLLRMYKMYAAKQGWKVTELD
                     FLEGDGAGIKTATLQIDGAFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVFVYPL
                     LDDSIEIEIKDSEVKMETARSGGAGGQNVNKVETKVFLTHIPTGITVICQTERSQIGN
                     REKAMQMLKSRLYEEELRKREELKNATNANKKKIEWGSQIRSYVFHPYKLIKDHRTDF
                     EVGNVGPVMDGELDGFIKAYLMISKEEEN"
     gene            complement(1203..1691)
                     /locus_tag="PLY81_10240"
     CDS             complement(1203..1691)
                     /locus_tag="PLY81_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF30649.1"
                     /translation="MRKLILVFLICFFHLSANSQTVQKVTINDVVNMMDTATVPTVIN
                     FWATWCGPCVKEIPWFEKLVEEYKKNNINIRLILVSLDFSTAYPNEISDFVKKKNFTS
                     KIVWLNETNADFYCPLIDKNWTGNIPVSIMVNNGTKFKEFYNSQLPEPRLRLELQKLI
                     AK"
     gene            complement(1715..>1939)
                     /locus_tag="PLY81_10245"
     CDS             complement(1715..>1939)
                     /locus_tag="PLY81_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF30650.1"
                     /translation="SGVSEEVRSLADGNFIIPQMGIIQSLNISVACAVSIYEAFRQKN
                     NAGHYLKSTMPQAQRNNLLKNWGFIEHDNN"
BASE COUNT          596 a          373 c          240 g          730 t
ORIGIN      
        1 atttatctgc tatttctaaa ataatttggt tatccattat ttcgtcaatt aaatttcttt
       61 agttctcctc ttctttgcta atcattaaat aagctttaat aaagccatct aattcaccat
      121 ccattacagg cccaacattg ccaacttcaa aatctgttct atggtctttt attaatttat
      181 aagggtgaaa aacatagctt ctaatttggc ttccccattc aatctttttt ttgtttgcat
      241 tagttgcatt cttcagttct tctctctttc ttaattcttc ttcatacaat ctgcttttaa
      301 gcatttgcat agctttttct ctattcccaa tttggcttct ttctgtttgg catattacag
      361 taatacctgt aggtatatgg gttaaaaaaa cttttgtttc aaccttgttt acattttgac
      421 caccagcacc accgctacgt gcagtttcca ttttaacttc actatctttt atttcaattt
      481 ctatagagtc gtcaagcaaa ggataaacaa aaaccgaagc aaagcttgta tgccttctag
      541 cattactatc aaatggcgaa attctaacta acctatgaac tccgctttct gcttttaaaa
      601 atccgtaagc aaaagcacca tctatttgca aagtagcagt ttttattcca gccccatccc
      661 cttcaagaaa atcaagttca gtaactttcc agccttgttt tgcagcatac attttataca
      721 ttcttaaaag catctctgcc caatcttgac tttcggtacc accagcacca gagtttattt
      781 gtaatacagc atttaactca tcttcgggct tatttaaggt acttttaaac tctgcattat
      841 ccagctcact tatagcatta ttaaaagcat taagggtttc ttcttctgtt gcatctgcag
      901 ctttgtaaaa ttcaaataaa acagcaaaat catcaatagc agtttctaca gtattgaata
      961 atttaaccca atactcattc acttttattt ctttcattat agaagtggct cttttattat
     1021 catcccaaaa gcctgcagag agagaaattt gtttatcctg ttctattttt gcaatacgat
     1081 tatcatagtc aaagaaacct cctcaggaca agtaagcgtt cctttatatc atttaatttt
     1141 tcatgtgtca taacatggca aaagtaagtg tatagctatt taagatacta ataatagaaa
     1201 agttattttg caattagttt ttgcaattct aatcttaacc ttggctctgg caattggcta
     1261 ttgtaaaatt ctttaaactt tgtaccatta ttaaccataa tacttacagg aatattacca
     1321 gtccagtttt tatcaattaa aggacaatag aaatctgcat tagtttcgtt tagccaaaca
     1381 attttacttg taaaattttt cttctttaca aagtctgaaa tttcattagg ataagcagta
     1441 ctaaaatcca aactcactaa aattaatctt atattgatgt tatttttttt atactcttct
     1501 acaagttttt caaaccaagg aatttcttta acacaaggcc cacaccaggt tgcccaaaag
     1561 ttaataacag tgggtactgt agctgtatcc atcatattta ccacatcgtt aatagttact
     1621 ttttgaactg tttgactatt tgcagaaaga tgaaaaaaac aaattaaaaa aacaagtatt
     1681 agctttctca tattttataa ttgtatgttg caagttagtt attatcgtgt tcaataaagc
     1741 cccagttttt taataaatta tttctttgag cctgtggcat tgtgcttttt aaataatgcc
     1801 cagcattgtt tttttgccta aatgcttcgt atatacttac tgcacaagca actgaaatat
     1861 ttaagctttg aataataccc atttgtggta tgataaagtt accatcagct aagcttctaa
     1921 cttcttctga tacaccact
//
LOCUS       DAOQHP010000398         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_47310_length_1928_cov_3.712760, whole genome shotgun sequence.
ACCESSION   DAOQHP010000398 DAOQHP010000000
VERSION     DAOQHP010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47310_length_1928_cov_3.712760"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="hflX"
                     /locus_tag="PLY81_10250"
     CDS             complement(<1..802)
                     /gene="hflX"
                     /locus_tag="PLY81_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNF30651.1"
                     /translation="MIEKQQKTNKQERVILVGVIQKEQTIEQVNEYMDELAFLAETAG
                     ALTVKRFTQKLQHPDSKTFVGKGKLEEIKNYIEGRNIDMVIFDDELTGSQLVNIEKVL
                     GIPIIDRSDLILDIFASRARTAQAKVQVELAQYQYILPRLKGMWKHLERQGGGIGTRG
                     PGETEIETDRRIVKDKISLLRKKLGEIDKQSFTQRKERGELIRVSLVGYTNVGKSTIM
                     NLLSKSDVFAENKLFATLDTTTRKVVFEATPFLLSDTVGFIRKLPHHLI"
     gene            complement(910..1686)
                     /locus_tag="PLY81_10255"
     CDS             complement(910..1686)
                     /locus_tag="PLY81_10255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30652.1"
                     /translation="MSNKIINTEQGHWLLAKMGKKVLRPGGKELTLKLIKGLKISNSD
                     NVIEFAPGIGYTAMKVLKFNPKSYTGVELNEEAATLLKRKINSNNRKIIIGNASNVML
                     EDASADKVYAEAMLTMQADHRKSEIIKEAYRLLKKGGLYGIHELGLTPDNLNEDTKAK
                     IQHELATCIRVNARPLTQSEWIHLLEKEGFKIVEINTNPMLLLETKRIIDDEGLFRTL
                     KIGFNILRFPKARKHILVMKEVFNKYKDQMNAISIIAQKA"
BASE COUNT          551 a          322 c          258 g          797 t
ORIGIN      
        1 caattaaatg gtgtggcagt tttcttataa acccaacagt atcgcttaat aaaaatgggg
       61 tggcttcaaa aacaactttt cgggttgtgg tatcaagtgt tgcaaataat ttattttctg
      121 caaaaacatc gcttttactt agcaggttca taatggtgct tttacccaca ttggtgtaac
      181 caactagcga taccctaatt aattctcctc tttcttttct ttgagtgaaa gattgtttat
      241 caatctcacc taattttttt cttaataaag aaattttatc ttttacaata cgtctgtctg
      301 tttctatttc tgtttctcca gggcctcttg ttcctatgcc acctccttgt ctttctaagt
      361 gtttccacat gccttttaaa cgaggtaaaa tatattggta ttgggctaat tctacttgca
      421 cttttgcttg agctgttctt gctctgcttg caaaaatgtc taaaataaga tcgcttctgt
      481 ctataatggg tattcctaaa actttttcaa tatttacaag ttgagagcct gttagttcat
      541 catcaaaaat taccatatca atatttcttc cttcaatata gtttttaatt tcttctaact
      601 ttcctttacc aacaaatgtt ttgctatcgg ggtgttgtag tttttgagta aatcttttaa
      661 ctgtaagtgc acctgcagtt tctgctaaaa aagcaagctc atccatatat tcattaactt
      721 gctctatggt ttgctctttt tgtattacac ctactaaaat tactctttct tgtttgtttg
      781 ttttttgttg tttttctatc aaaaggtttt agttttttat gaactgcaaa attagatgaa
      841 ttgtttgcag ctattaacat attaaacaag aaaataaact ttatttaagt tgagaagaaa
      901 tgaaccagct tatgcttttt gagcaataat agaaatagca ttcatttggt ctttgtattt
      961 attaaacact tctttcatta caagtatgtg ttttcttgct ttcggaaatc ttaaaatatt
     1021 aaacccaatt tttaaagttc taaataatcc ttcatcatca ataattcgtt tggtttctaa
     1081 aagcaacatt gggttagtgt ttatttctac aattttaaat ccttcttttt ctaaaagatg
     1141 tatccattcg ctttgtgtta aaggtcttgc attaactctt atacaagtgg caagttcatg
     1201 ctgaattttt gcttttgtat cttcattcaa attatctgga gttaggccca attcatgtat
     1261 gccatataaa ccaccttttt tcaaaagcct gtaagcttct tttataattt cagattttct
     1321 atggtctgct tgcatggtta gcattgcttc tgcataaact ttatctgcac ttgcatcttc
     1381 taacattaca ttagaggcat taccaataat aatttttctg ttgttgctgt ttatttttct
     1441 tttcaaaagt gtagcggctt cttcatttag ttctacacct gtataagatt ttggattaaa
     1501 ctttaaaacc ttcatagctg tataacctat acctggtgca aactctatta cattatcact
     1561 attagatatt tttaagccct taattagctt tagtgttaat tctttacctc caggccttag
     1621 aacttttttg cccatttttg ctaacagcca atgtccttgc tctgtattaa ttattttatt
     1681 actcataaaa ttttaatttg tattgaaagc tacaaaattc atttacaagc aatgctatta
     1741 ttatctaaat aagaaatttg atttattgaa tgaggaatat catttttaat atcttctgaa
     1801 gcacttgata caagtgcatt acaagatgtt ttattttttt attgtaaaat atttttttaa
     1861 aaaatgatga caaatttatg acaggtattg tgctgtaaat tttgtgccca ataaaaccat
     1921 tattccta
//
LOCUS       DAOQHP010000399         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_47614_length_1921_cov_4.382637, whole genome shotgun sequence.
ACCESSION   DAOQHP010000399 DAOQHP010000000
VERSION     DAOQHP010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47614_length_1921_cov_4.382637"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..293)
                     /locus_tag="PLY81_10260"
     CDS             complement(21..293)
                     /locus_tag="PLY81_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30653.1"
                     /translation="MTEKEPILFQNDCNEQEMFDYLNNNLTPQQRHAFELKMEENVFL
                     QEAVEGLEKIQNKQKVHQFITELNHNLDKQLERKRIKKHKRKNIPT"
     gene            complement(290..859)
                     /locus_tag="PLY81_10265"
     CDS             complement(290..859)
                     /locus_tag="PLY81_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF30654.1"
                     /translation="MQPANYQHITDKELLNLYNSSNENKWIGILLQRYTLLLFGICFK
                     YLKNEEAAKDAVQQIFLHAIAEIKKYEITYFKSWIYVLARNYCLMQLRKKNVYIEITD
                     SHVDENDTKYNIEKLLEKEETLNLLEKALQQLNQEQKTCLSLFYLQKKTYQQVSEATG
                     YSLLQVKSFIQNGKRNLKLLIQKNSTQKI"
     gene            1076..>1921
                     /locus_tag="PLY81_10270"
     CDS             1076..>1921
                     /locus_tag="PLY81_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF30655.1"
                     /translation="MAARADLFQSPDYFALDELLSEEHKLIRETVRNYVKKEISPIIE
                     DYAQRAEFPEQIVKQMGELGCFGPTVSVEYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMYPIYAYGSEEQKRKYLPKLANGEFLGCFGLTEPNHGSDPSSMLTTIKDAG
                     DHVILNGAKMWISNAPYAHVAVVWAKDEHNIVRGLIVERGMPGFSTPTTHGKWSLRAS
                     ATGELVFDNVKVPKENIFPNVQGLKGPLGCLTKARFGIAWGALGAAMDCYDTALRYSK
                     ERIQFD"
BASE COUNT          619 a          257 c          320 g          725 t
ORIGIN      
        1 atctatattt tgacgtccaa tcaggtaggt atattttttc ttttatgttt ttttatcctt
       61 ttgcgttcta attgtttatc caggttatgg ttcagttcag taataaattg atgtactttt
      121 tgtttgtttt gtatcttttc taaaccttcc actgcttctt gcaaaaaaac attctcttcc
      181 attttaagct caaatgcatg gcgttgttga ggtgttaaat tattattcaa ataatcaaac
      241 atttcttgtt cgttacagtc gttttgaaac aatattggct ctttttctgt catatttttt
      301 gagtcgagtt tttttgaatt agaagcttta aatttctttt gccattttgt ataaaacttt
      361 taacctgtaa taaagaataa cctgttgctt ctgatacttg ctgataggtt ttcttttgta
      421 aataaaataa cgataaacat gttttttgtt cctggtttaa ttgttgtaaa gccttttcta
      481 ataagtttaa tgtttcttct ttctccaata gtttttcaat attatatttt gtatcatttt
      541 catcaacatg agaatcggtt atttcaatat acacattttt ctttcttaat tgcattaaac
      601 aatagtttct ggccaacacg taaatccaac ttttaaaata agttatttca tattttttta
      661 tttcggcaat tgcatgcaaa aatatttgtt gtacagcatc tttggctgcc tcttcgttct
      721 ttaaatattt aaagcaaatg ccaaaaagta ataatgtata tcgttgtaat aaaattccaa
      781 tccatttatt ttcattactg gagttgtaaa gatttaaaag ttctttatca gtaatatgtt
      841 ggtagttagc aggctgcact atacaccttt attgcatagt aaacttaata aagtttaatt
      901 aatttttcta attaaagagt aatttttttg agaagttttt tgctgtttaa tgccaatctt
      961 taaaaccaaa taaaatagaa tttattcact tacttttgca taaattttta aagaaatttt
     1021 aataccaact ttaactttgg tttaattcat ttcatcaaca aaaaaaatat tttacatggc
     1081 agcaagagca gatttatttc aaagcccaga ttattttgca ttagacgaat tattatcaga
     1141 agagcacaag ttaattagag aaactgttcg taattatgtt aaaaaagaaa tatctccaat
     1201 tatagaagat tatgctcaac gtgccgaatt tccagaacaa attgttaaac aaatgggtga
     1261 gttaggatgt tttggtccaa ctgtttctgt tgaatatggt ggtggaggtt tagattatat
     1321 ttcttacgga ttaatgatgc aagaattaga aagaggagat agtggagtaa gaagtactgc
     1381 aagtgtacaa ggcagtttag ttatgtatcc tatatatgcc tatggtagcg aagaacaaaa
     1441 aagaaaatat ttacctaaac ttgcaaatgg tgaatttctt ggatgttttg ggctaacaga
     1501 acctaatcat ggtagtgacc ctagtagtat gctaactacc ataaaagatg caggagatca
     1561 tgtaatttta aatggagcaa aaatgtggat tagcaatgca ccttatgctc atgttgcagt
     1621 tgtatgggca aaagatgaac ataatatagt aagaggccta attgtggaac gtggaatgcc
     1681 tgggttttct accccaacaa cacatggtaa atggagttta agagctagtg caacaggtga
     1741 attagttttt gataatgtaa aagtacctaa agaaaatatt tttcctaatg tacaaggctt
     1801 aaaagggcca ttgggctgtt taactaaagc aagatttgga attgcttggg gtgctttagg
     1861 ggctgcaatg gattgctacg atactgcttt aagatatagt aaagaaagaa tacaatttga
     1921 t
//
LOCUS       DAOQHP010000400         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_47717_length_1919_cov_3.075107, whole genome shotgun sequence.
ACCESSION   DAOQHP010000400 DAOQHP010000000
VERSION     DAOQHP010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47717_length_1919_cov_3.075107"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLY81_10275"
     CDS             <1..335
                     /locus_tag="PLY81_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30656.1"
                     /translation="VSSKFTYGGGKRYSPVDIAASNAVMDVVADNDKVNTLQFNPYNR
                     LDLRIAYKINGKKAAYEIALDLVNLMNTKNVLALSYAPDPANINADPLIKNYQLGFLP
                     LFYVKVDF"
     gene            316..1071
                     /locus_tag="PLY81_10280"
     CDS             316..1071
                     /locus_tag="PLY81_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNF30657.1"
                     /translation="MLRWISKISIALLFLATPSFAQVINIENKRFLNDTNGWVGNIDF
                     NFNVYNNTQQVLQFSNAARVQYQKNRSRFILLNDLNFIKAGNTDFANAGYQHFRYNYK
                     VSKWLTMEAFTQTQYNPVLKLDFRYLLGAGPRLKLLKKENARIYLAALYMYEYDDIVD
                     NAVNLYEHRLSSYLTFSFALFKTLDLTSTTFYQPNIENTSDYRIANDSGLEIHINKHL
                     NFKSTFNMLYDTKQPIGIPDLVYSFKNGLSIKF"
     gene            complement(1075..1281)
                     /locus_tag="PLY81_10285"
     CDS             complement(1075..1281)
                     /locus_tag="PLY81_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30658.1"
                     /translation="MKKVYFIVSILTLSIAFIKCKPAAEDRVKMHVNANRIADSIGKV
                     IDAAMAEVAIKPKEGATPSDTSKK"
     gene            complement(1412..1657)
                     /locus_tag="PLY81_10290"
     CDS             complement(1412..1657)
                     /locus_tag="PLY81_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30659.1"
                     /translation="MKKVFSIIAVALIATSVVSCGPSAEEKAKIEEQAKHTADSISAA
                     LSASLEGAMNEATADTTAAAATTETAAPAAEAAHAGH"
BASE COUNT          653 a          267 c          317 g          682 t
ORIGIN      
        1 atgtatcttc taaatttact tatggcggag gcaaaaggta ttcacctgtt gatatagctg
       61 caagtaatgc agttatggat gtggttgcag ataatgataa agtaaacact ttacaattta
      121 atccatataa tcgtttggat ttgcgaatag cttataaaat aaatggtaaa aaagctgcat
      181 acgaaattgc cttggatttg gtgaatttaa tgaatacaaa aaatgtatta gctttaagtt
      241 atgctccaga tcctgcaaat attaatgctg atccattaat caaaaattac cagttaggat
      301 ttttaccttt gttttatgtt aaggtggatt tctaaaataa gtattgcttt attatttctt
      361 gcaacacctt catttgctca ggttataaat attgagaata agcgcttttt aaatgatacg
      421 aatgggtggg taggaaatat tgattttaat tttaatgttt ataacaatac acaacaggtt
      481 ttgcaatttt caaatgctgc tagagttcag tatcaaaaaa acagaagtcg ttttatttta
      541 ttaaatgatt tgaattttat taaagcaggt aatactgatt ttgcaaatgc gggctatcag
      601 cattttaggt ataattacaa ggtgagtaaa tggcttacga tggaagcttt tactcaaaca
      661 caatacaatc cggttttaaa attagatttt aggtatttgt taggtgcagg tccgaggttg
      721 aaattattga aaaaagaaaa tgccagaatt tatttagcag ctttatatat gtatgaatac
      781 gatgatatag ttgataatgc agttaattta tatgagcatc gattgagttc gtatcttacc
      841 tttagttttg ctctttttaa aacgcttgat ttaaccagta ctaccttcta ccagccaaat
      901 atagaaaata cgtcagatta tagaatagcc aatgacagtg gtttggagat acacattaat
      961 aagcatttga atttcaaatc aacatttaat atgttgtatg acacaaaaca accaatagga
     1021 ataccagatt tggtatattc ctttaaaaat ggtttatcaa taaaatttta gaaattattt
     1081 tttgctagtg tcagaaggtg tagcaccttc ttttggctta atagcaactt ctgccatcgc
     1141 agcatcaata acttttccaa tggaatcagc aatacgattt gcattcacat gcattttaac
     1201 tctgtcttcg gcagccggtt tacattttat aaatgcgatg gataaagtta aaattgatac
     1261 aataaaatat acttttttca taaatgtctg attgatattt cgaatgtaaa gaaaattttt
     1321 tgttggataa agaaattatg attatttata aaataaaaag cccacgtatc tgggatacgt
     1381 ggacttttcc taactaacta aacacaaatt cttagtgtcc tgcgtgagca gcttcagcag
     1441 ctggagcagc agtttcagta gttgcagcag cagcagttgt atcagctgta gcctcattca
     1501 ttgcaccttc taagctagca gataatgcag cagagataga atcagcagta tgttttgctt
     1561 gttcttcgat ttttgctttt tcttcagctg aaggtccaca agatactaca gatgtagcga
     1621 ttaaagctac tgcgataatt gaaaatactt ttttcatttg attttaattt ttttaagttg
     1681 ttttgttgtt atttgaacat ttataccgaa actttaaaat ggtaacccga aaaaagaata
     1741 aaataattaa ttattttata actaactgat tatcaatgat taaaatttaa aataaaaata
     1801 ggttaccacc tttttagaag gattgtattt aagataaaac gtaaaaaaag ggtttatttt
     1861 gctaaaaaat ctcctactat tttttcagct tttgcaaaag cgtcctctaa cacttcatt
//
LOCUS       DAOQHP010000401         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_47781_length_1917_cov_4.902256, whole genome shotgun sequence.
ACCESSION   DAOQHP010000401 DAOQHP010000000
VERSION     DAOQHP010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47781_length_1917_cov_4.902256"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1720
                     /locus_tag="PLY81_10295"
     CDS             113..1720
                     /locus_tag="PLY81_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF30660.1"
                     /translation="MFWNNYPHEVKAVIRFLEILKIKVNRDSVNQTLQSHPDWPSLLC
                     ISDSLKSWQIQNEAFKIETLELERLEVPFIAYMPKELTPFAIVTKINETKLTIYYGNY
                     NKPLNILKKDFLNKWDGTILIANKEEFSGEQNFKMNKRKQILKRMMSLSLISLVMVLI
                     SFYLFRTSEQYEEIGIVFISIQYFILLCGAFISTLLLQYEIFLTNHFLDKVCGSIKHG
                     NCSAILKSKGAKFLNLLSWSDVGFIYFGGSLLILVTLHILDAIHILAIINLVALPYIL
                     FSVYYQWKVLKQWCILCLIIQGLLLAGFLNVFFNNLLFVSPISKQALIEAAILYLMTA
                     FALFLIKPLFVKIKESKDEKIQLLRLKFNTDVFTSILEKQKSIELPPDGLGILLGNPH
                     AENEIIKVCNPYCNPCRKAHQKIGNILHKNKNIRVRLIFNATNKPNDFRAIVVKHFLA
                     LYSEGDNIKIQKALNVWYNDEYITYEKFSVLFPKEKNQDYGVNEINSMDNWCKTNEIS
                     FTPTFFIKGKQIPNMYNIEDINYFLME"
BASE COUNT          731 a          269 c          304 g          613 t
ORIGIN      
        1 ctgcaagtgc cccaataagt agatgatgca atcgggcaca ccgttagcat tttagtaaaa
       61 aagtatcttg aatttaccat gttaaccaaa actattttaa catagaataa caatgttctg
      121 gaataattat ccacatgaag taaaagcagt aataagattt cttgaaatat taaaaattaa
      181 agtaaatcgt gattcagtca atcaaacatt gcagagtcat ccagattggc ccagcttact
      241 atgcatcagt gatagtctga aatcatggca aattcaaaat gaagcattta agatagagac
      301 cttagaatta gaaagattag aagttccatt tatagcatat atgcctaagg aattgactcc
      361 attcgctata gtcactaaaa ttaatgaaac aaaactaact atttattatg gcaattacaa
      421 taaaccctta aatatattaa aaaaagactt tctaaataaa tgggacggca caatccttat
      481 tgcaaacaaa gaagaatttt caggagagca gaattttaaa atgaataaaa gaaaacaaat
      541 actaaaaaga atgatgtctt tatcattaat tagtttagtg atggtattaa tttcgtttta
      601 tttatttaga acatcagagc aatatgaaga aattgggatt gtattcatta gtattcaata
      661 ttttattttg ctatgtggag catttattag tacactatta ttgcagtatg aaatattttt
      721 aaccaaccat tttcttgata aggtctgcgg aagtataaaa catggtaatt gtagtgcaat
      781 tcttaaaagc aagggtgcga aatttttaaa cttactgtca tggagtgacg ttggatttat
      841 atacttcggc ggcagtctac tcatactagt aacattacac atattagatg caattcacat
      901 attggcaatt attaatttgg tagcgcttcc ttatatcctc ttttcagttt actatcaatg
      961 gaaagtctta aaacaatggt gcattttgtg cttaattatt cagggcctat tattggcagg
     1021 cttcttaaat gtatttttta ataacttatt atttgtatct ccaattagca agcaagcgct
     1081 gattgaagca gcaatcttat atttaatgac agcttttgct ttgtttttaa taaagccatt
     1141 atttgttaaa ataaaggaaa gtaaagatga gaaaatacaa ctattaagac taaaatttaa
     1201 tactgacgtt tttacaagca ttttagaaaa gcaaaaaagt attgaattgc ctccagatgg
     1261 tcttggtatt ttattaggga acccacatgc agaaaatgaa ataattaaag tatgtaatcc
     1321 gtattgtaat ccttgtagaa aagctcatca aaaaattgga aatattttgc ataaaaataa
     1381 aaatataaga gttaggctca tttttaatgc cacaaacaaa ccaaatgact tcagagcaat
     1441 tgtcgtcaaa cactttcttg cgctatattc agaaggagat aatattaaaa ttcaaaaagc
     1501 attaaatgtg tggtataatg atgaatatat tacttacgaa aaattctctg tactattccc
     1561 aaaagagaaa aatcaagatt atggtgttaa tgaaataaac tcaatggaca attggtgtaa
     1621 gactaatgaa atttcattta ctcctacatt ttttattaaa ggaaaacaaa tacctaatat
     1681 gtacaatatt gaggatataa attattttct tatggagtag ctcaccatga gaaaattctc
     1741 ggcagagctg gttctgctgt gtacaggcag gacagccgag aggccaagac cattgaagcc
     1801 aaaagctatg gcgtaaaata tcttttaacc ttaaattttt taaaaatgga acaaaacaaa
     1861 agtaatctct taatctttaa aaggaggctg actagagaac aaatgaagaa cattaac
//
LOCUS       DAOQHP010000402         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_47886_length_1915_cov_3.642473, whole genome shotgun sequence.
ACCESSION   DAOQHP010000402 DAOQHP010000000
VERSION     DAOQHP010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47886_length_1915_cov_3.642473"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLY81_10300"
     CDS             <1..232
                     /locus_tag="PLY81_10300"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNF30661.1"
                     /translation="KTNGGIVEIHLIVEKGIIKEAKIYGDFFNLKDIAEIENALQNSR
                     HDENSIRKIFENFVIDEYFMNITLDEIISAMF"
     gene            332..1522
                     /gene="kbl"
                     /locus_tag="PLY81_10305"
     CDS             332..1522
                     /gene="kbl"
                     /locus_tag="PLY81_10305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNF30662.1"
                     /translation="MNFNFSERIKSELAEIEAASLFKKERIITSEQGAEIVVNGKTVL
                     NFCANNYLGLSSHPKVIEAAHKAIDSHGYGMSSVRFICGTQDIHKTLEQKIAQFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDALNHASIIDGVRLCKAQRYRYEHNNMEDL
                     ENKLKETANLRNRIIVTDGSFSMDGTIAQLDKICDLADKYDAIVMIDECHSSGFLGKT
                     GRGTHEYRNVMGRIDIITGTLGKALGGASGGFTSGKKEVIEMLRQRSRPYLFSNTVAP
                     SIVGASIAVLDMLTETTELRDKLEFNTKYFRSKMTEAGFDIKPGDHPIVPIMLYDAVV
                     AQNFAAKLLDEGIYVIGFFYPVVAKGLARIRVQLSAAHNQAHLDKAINAFIKIGKELN
                     VLKG"
     gene            1649..>1915
                     /locus_tag="PLY81_10310"
     CDS             1649..>1915
                     /locus_tag="PLY81_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNF30663.1"
                     /translation="MRSLITVIVLFSTILVACSPNNIKEDKSLKKYFEENKVEGTFGI
                     FDNGKGDFTIYNLGRYKDSAFTPASTFKIVNALIGLQTGAITNEK"
BASE COUNT          710 a          245 c          337 g          623 t
ORIGIN      
        1 caaaaccaat ggtggtatag tagaaataca tttaattgta gaaaaaggaa taattaaaga
       61 agcgaaaatt tatggcgatt tttttaattt gaaagatatt gcagaaatag aaaatgcact
      121 tcaaaattcc aggcatgatg aaaactcaat aagaaaaatt tttgaaaatt ttgttataga
      181 tgaatatttt atgaatatta ccttagatga aattattagt gctatgtttt aaaaaccaat
      241 atagtaacag tgtaaatata ctttttctgc tataactttt aaaatcctct aatcctcaac
      301 cttatctttg taaccttaaa ttataataac aatgaatttc aatttttcag aaagaataaa
      361 atcagagctt gccgaaattg aagctgccag tttatttaaa aaagaaagaa ttattactag
      421 cgaacaaggt gctgagattg ttgtgaatgg caaaactgtt ttaaactttt gtgctaataa
      481 ttatttaggt ttatcctctc atcctaaagt aatagaagct gctcataaag caatagatag
      541 ccatgggtat ggaatgagca gtgtacgttt tatttgtggc acacaagata ttcataaaac
      601 cttagagcaa aaaattgcac agtttttagg aacagaagat accattttat atgcagctgc
      661 atttgatgca aatggtggtg tttttgaacc attgtttaat gaacaagatg ctattataag
      721 tgatgcgtta aatcatgcaa gtattattga tggagttcgt ttatgtaagg ctcaacgtta
      781 cagatacgag cacaataaca tggaagattt agaaaataaa ttgaaagaaa cagcaaatct
      841 tagaaataga attattgtta ccgatggtag ctttagcatg gatggcacca ttgcacaatt
      901 agataaaatt tgcgacttag cagataaata tgatgctatt gtaatgatag acgaatgtca
      961 ttcatctggc tttttaggaa aaacaggaag aggaacacac gaatacagaa atgtgatggg
     1021 tagaattgat attattactg gtactttagg caaagcttta ggtggagcaa gtggtggctt
     1081 tacaagtggt aaaaaagaag ttatagaaat gttacgccaa cgttctcgtc catatttatt
     1141 ttcaaacaca gttgcaccaa gtattgtagg tgctagcatt gctgtgttag atatgcttac
     1201 agaaactact gagctaagag ataaattaga atttaataca aaatatttta gatcaaaaat
     1261 gacagaagca ggttttgata ttaaacctgg cgatcatcct attgtaccca ttatgttgta
     1321 cgatgcagtt gtagcacaaa attttgcagc aaaattatta gatgagggca tctatgtaat
     1381 aggcttcttt tatcctgttg tagctaaagg tttggcacgt attcgtgtac aattaagtgc
     1441 agcacataat caggctcatt tagataaagc cattaatgca tttataaaaa ttggtaaaga
     1501 actaaatgtt ttaaaaggct aactgcattt ttattcttga agataatgca atgtaaaatc
     1561 gggattttat acccgatttt ttattttgaa taaacagtaa aaagcaacat tttaacttaa
     1621 atttgcaaac tcaattaaaa acaatttcat gcgaagttta ataactgtga tcgttttatt
     1681 ttctactata ttagttgcat gctcaccaaa taatattaaa gaagataaga gcttaaaaaa
     1741 atattttgaa gaaaataaag tagagggaac tttcggaatt tttgataatg gtaaaggaga
     1801 ttttactatt tacaatttag gccgttacaa agattcagct tttactcctg catctacttt
     1861 taaaattgta aatgctttaa ttgggttaca aacaggggca attaccaatg aaaaa
//
LOCUS       DAOQHP010000403         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_48560_length_1900_cov_2.844444, whole genome shotgun sequence.
ACCESSION   DAOQHP010000403 DAOQHP010000000
VERSION     DAOQHP010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48560_length_1900_cov_2.844444"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLY81_10315"
     CDS             complement(<1..327)
                     /locus_tag="PLY81_10315"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNF30664.1"
                     /translation="MFTNSITSLFKIQYPIIQAGMIWASGWRLASAVSNAGGLGIIGA
                     GSMYPAVLKEHIKKCKAATNKPFAVNLPLLYPSIEEHIKIIMEEKVPIVFTSAGNPKT
                     WTSLLKE"
     gene            638..1285
                     /locus_tag="PLY81_10320"
     CDS             638..1285
                     /locus_tag="PLY81_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30665.1"
                     /translation="MKKIIIVLLMFLYSCNQNNENVSLLEDVYSIPSYMRYAASSNFS
                     ENTDAEHNYKLLDTLKWMHESKLQTWNSKKMWFVLKKEMSDTLLKTKQENVVSFAKAY
                     KALVLLKYTDLLQQQDDEAIQEMKDCLEQLIAWQYGGYHIYYATIISLKNHIPNKELK
                     GYINESLAIENPLFTVHVRDADTDDEKAVSSLLGTNKLISNIQKMKLYKEKLAIL"
     gene            complement(1310..>1900)
                     /locus_tag="PLY81_10325"
     CDS             complement(1310..>1900)
                     /locus_tag="PLY81_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF30666.1"
                     /translation="YKGSAQNHHYVIENVVEKPAFPKSNLALVGLYKIKDSSLLYKCL
                     QHLFVEDIKTQGEYNLTDALDCMIQRGAKFQSFKVKNWFDCGKKETLIESNATLLKKL
                     GGNTTSTHQFEDTIIIPPVSIAENCHIKNAVIGPNVSIGNNTSILNSVVHNSIIGSFT
                     SIKQVVLNNSLIGSDAHVKGLSKSLNIGDNTEIEFG"
BASE COUNT          695 a          275 c          267 g          663 t
ORIGIN      
        1 ttcttttaat aagctagtcc atgttttagg atttccggca cttgtaaaaa caatcggaac
       61 tttttcttcc ataattattt ttatatgttc ctcaatagaa gggtaaagca aaggcaagtt
      121 tactgcaaaa ggtttattgg ttgctgcttt acatttttta atatgctctt ttaaaacagc
      181 aggatacata cttcctgcac caataatacc taaaccacct gcattactta cagcacttgc
      241 taaacgccaa ccacttgccc aaatcattcc tgcttgtata atgggatatt gaattttgaa
      301 taaagatgta atgctattgg taaacataaa tgataaaaaa taaattaatt tttagtgaaa
      361 ataagttttt gaaataatgt tttaaatatt gctactacat ggcttaataa aaacaatcaa
      421 cctgtacaat gttaaagcaa atttcgataa aattaataac taattgaaag aattcttcat
      481 taatgaaact aattaaattg aggaagtaaa gtacaatatt tttgcaccaa accctttgtg
      541 ggcttcaaaa aaatatattt ggaaatgtca tttttttttt ttctttcgta tgcaaatgcc
      601 attcattaca ttattaacta acttaaaaca attaattatg aaaaaaataa ttattgttct
      661 attaatgttt ctttatagtt gtaatcaaaa caatgaaaat gtaagcctat tagaagatgt
      721 ttattccatt ccatcttata tgcgttatgc tgctagtagc aatttttcag aaaatacaga
      781 tgctgaacat aattacaaat tattagacac tttgaaatgg atgcatgaaa gcaaattgca
      841 aacgtggaat tctaaaaaaa tgtggtttgt tttaaaaaaa gaaatgagtg atacattatt
      901 gaaaacaaaa caagaaaatg ttgtttcatt tgcaaaagcc tacaaagcac ttgtattact
      961 taaatataca gatcttttgc agcaacaaga tgatgaagca atacaggaaa tgaaagattg
     1021 tttggaacag cttattgcat ggcaatatgg tggttatcat atttactatg ctacaattat
     1081 aagccttaaa aatcatatac ctaataaaga gttaaaagga tatattaacg agagtttagc
     1141 tattgaaaat ccattgttta ctgttcatgt aagagatgct gatactgatg atgaaaaggc
     1201 agtatctagc ttgttaggta caaacaaact aataagcaat attcaaaaaa tgaaattgta
     1261 taaagaaaaa cttgccattc tataataata ctggcattat taaaatactc tatccaaatt
     1321 ctatttctgt attatcgcca atatttaaac ttttgcttaa tcctttaaca tgggcatcgc
     1381 taccaattaa tgaattattt agaactactt gtttaataga tgtaaatgag ccaataatac
     1441 tattatgtac aacagagttt aatatagaag tgttattacc aatagagaca ttgggaccaa
     1501 ttacagcatt ttttatgtgg caattttcag caatacttac aggaggtata ataatagtat
     1561 cttcaaattg atgggtagaa gtagtattgc caccaagttt ttttaaaaga gtagcattgc
     1621 tttcaattaa tgtttctttt ttaccacaat cgaaccagtt tttaacttta aaactttgaa
     1681 actttgctcc acgctgtatc atacaatcta aggcatcggt taaattatat tcaccttgcg
     1741 tttttatatc ctcaacaaat aaatgttgca ggcatttata taataggctg ctgtctttaa
     1801 ttttatataa tcccactaaa gccaaattac ttttcggaaa tgctggtttt tctacaacat
     1861 tttcaataac atagtgatga ttttgtgccg agcctttata
//
LOCUS       DAOQHP010000404         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_49097_length_1888_cov_3.563557, whole genome shotgun sequence.
ACCESSION   DAOQHP010000404 DAOQHP010000000
VERSION     DAOQHP010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49097_length_1888_cov_3.563557"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLY81_10330"
     CDS             complement(<1..434)
                     /locus_tag="PLY81_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30667.1"
                     /translation="MVKIVVVFFVLLFTLGCNQQPNVNENKNVEKPTIIGLWEVKFIC
                     ETDFTMDSRDTLRGTFCHSINNQFIKFNKDSIFYIEDSLITDTYKYSKYNDSVLNVQN
                     IKTGIQMKMYIQNITLSNLKFKNIRIDSNRQEEYEIMIDCIKT"
     gene            complement(447..>1888)
                     /locus_tag="PLY81_10335"
     CDS             complement(447..>1888)
                     /locus_tag="PLY81_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF30668.1"
                     /translation="NVKQYARHEMLDIVEIDGKARGIIARDLISGKLQRHFGNAVLLC
                     TGGYGNVFYLSTNAMGSNVTAAWKAHKAGALFANPCFTQIHPTCIPVSGDHQSKLTLM
                     SESLRNDGRIWVPKNQGDNRKPNEIPESERDYYLERRYPAFGNLVPRDVASRAAKERC
                     DAGFGVGTSKQAVYLDYASAIERYGKIEITKHGREVEDASSAEIITLGKQVVKEKYGN
                     LFDMYEKITGENPYEVPMRIYPAVHYTMGGLWVDYELMTSVKGMYCLGEANFSDHGAN
                     RLGASALMQGLADGYFVIPYTIGNYLADEIYSKPISTDHEAFVNAEKEVGDRIRTLMN
                     IKGNTSVETFHKRLGKIMWDKCGMARNEKGLQEAIVEIQQLRKEFWNDVRIPGDIDEF
                     NPELDKAGRVADFLELGELMCKDALLRKESCGGHFREESQTEEGEALRNDAEFMYVAA
                     WEFEGESNWKLHKEALEYEVIKPTQRNYK"
BASE COUNT          553 a          390 c          216 g          729 t
ORIGIN      
        1 gttttaatac aatctatcat tatttcatat tcttcttgtc tattactatc tattctaata
       61 tttttaaact ttaagttaga taatgttata ttctgaatat acattttcat ttgaatacct
      121 gttttaatat tttgaacatt taaaactgaa tcattatatt tagaatattt gtaagtatct
      181 gttattaaac tatcttcaat ataaaatata ctatccttat taaatttaat aaactgatta
      241 tttattgaat ggcaaaatgt tcctcttaac gtatctctgc tatccatcgt aaaatctgtt
      301 tcacaaataa atttaacttc ccaaagacct attattgttg gtttttctac atttttgttt
      361 tcatttacat tgggttgttg attacaacct aaggtaaata atagcacaaa aaaaacaaca
      421 actattttaa ccataacttc tataaactat ttatagtttc tctgcgttgg tttaattact
      481 tcatactcta aagcttcttt atgtaatttc caattgcttt ctccttcaaa ttcccaggct
      541 gctacataca taaactctgc atcatttctt aaagcttctc cttcttcggt ttggctttct
      601 tctctaaaat ggccaccaca actttctttt cttaataatg catctttaca cattagttct
      661 ccaagttcta aaaagtctgc aaccctacca gctttgtcta attctggatt aaattcatca
      721 atatctcccg gtattcttac atcattccaa aattcttttc ttaattgttg tatttctaca
      781 atagcttctt gtaaaccttt ttcatttctt gccattccgc atttatccca cattatttta
      841 cctaaacgtt tatgaaaggt ttctaccgat gtgttgccct taatattcat caaggttctt
      901 attctgtctc ctacttcttt ctctgcattt acaaatgctt catgatcggt agatataggt
      961 ttagagtata tttcatctgc caaataatta ccaattgtat atggtataac aaagtatcca
     1021 tctgccaaac cttgcattag tgcactagcc ccaagcctat tagctccatg atcgctaaaa
     1081 tttgcttcgc ccaaacaata cattcctttt acacttgtca ttaattcata atcaacccat
     1141 aaaccaccca ttgtatagtg tactgctggg taaattctca ttggtacttc ataaggattt
     1201 tctccagtaa ttttttcata catatcaaac aggtttccat atttttcttt aactacctgt
     1261 ttacctaatg taataatttc agcactgcta gcatcttcta cttctctgcc atgtttagta
     1321 atttcaattt taccatatct ttcaattgcc gaggcataat ctaaataaac agcttgttta
     1381 cttgtaccta cgccaaagcc tgcatcacat ctttctttag cagcacggct tgcaacatct
     1441 cttggaacaa ggttaccaaa tgcaggatat cttctttcta aataataatc tctttctgat
     1501 tctggtattt cattaggttt tctattatct ccctgatttt ttggaaccca aattctacca
     1561 tcattcctca acgattcgga cattaaggtt agtttacttt ggtggtctcc actaactgga
     1621 atacaagttg gatgaatttg tgtaaaacaa ggatttgcaa ataatgcacc agctttatgt
     1681 gccttccatg cagcagtaac attgctaccc attgcattgg tgcttaaata aaatacgttg
     1741 ccataaccac cagtacaaag taaaacagca tttccaaaat gtcgttgcaa cttaccagaa
     1801 attaaatctc ttgcaataat accacgtgct ttgccatcga tttctacaat gtctaacatt
     1861 tcgtggcgtg catattgttt tacattac
//
LOCUS       DAOQHP010000405         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_49490_length_1880_cov_3.729863, whole genome shotgun sequence.
ACCESSION   DAOQHP010000405 DAOQHP010000000
VERSION     DAOQHP010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49490_length_1880_cov_3.729863"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="nuoI"
                     /locus_tag="PLY81_10340"
     CDS             complement(<1..493)
                     /gene="nuoI"
                     /locus_tag="PLY81_10340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNF30669.1"
                     /translation="MQTLTNRSKQVDRSPMNFLERIYLWNIFKGMFITFKHMFKKQPT
                     IHYPEQKRTFSPVFRGLHVLNRDEEGRERCTACGLCAVACPAEAITMEAAERLPEEEN
                     LYREEKYAAKYEINMLRCIFCGLCEEACPKDAIYLSETLAPSNYSRKNFIYSKQDLLI
                     PNPK"
     gene            complement(710..1774)
                     /gene="nuoH"
                     /locus_tag="PLY81_10345"
     CDS             complement(710..1774)
                     /gene="nuoH"
                     /locus_tag="PLY81_10345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNF30670.1"
                     /translation="MTFLTIDWLFIIEKLVLIIIIILASLGIALYTTFAERKVAAILQ
                     DRPGPNRAGPFGLLQPLADGVKMFTKEEIVPTQASKWLFILGPGLAMVASLMTCAVIP
                     WGGSVELFDRKIDLQVADINIAVLYIFAVVSLGIYGIMIGGWASNNKFSLMAVLRAAS
                     QAISYELAMGLSLIAVLMITGTLSLNEMVKQQQEGLWNIVYQPLGFLLFFICALAECN
                     RTPFDLPEAESELNFGYHQEYSSMKLGFYLFAEYINMIMSSAVMATLYFGGYDIPFVN
                     DANLYASIGNWAALLQVTSILIKIVIFIFVFMWIRWTIPRFRYDQLMDLGWKKMVPLA
                     LFNMLVTAFIVLLKHNGWKF"
BASE COUNT          606 a          357 c          259 g          658 t
ORIGIN      
        1 ttttaggatt tggtattagc aaatcttgtt tactataaat aaaattcttt ctgctataat
       61 tagatggtgc caaagtttcg cttaaataaa ttgcatcttt ggggcaagct tcttcgcaaa
      121 ggccacaaaa aatgcaacgc agcatattta tttcgtactt agctgcatac ttttcttctc
      181 tgtataaatt ttcttcttcg ggtaatcttt ctgcagcttc cattgtaata gcttctgctg
      241 ggcaagctac tgcacaaagc ccacaagctg tacaacgttc tctgccttct tcatctctat
      301 taagtacatg aagccctcta aaaacaggac taaatgttct tttttgttca ggatagtgaa
      361 tagtaggttg ctttttaaac atgtgtttaa aagtgatgaa catcccttta aaaatattcc
      421 ataaataaat ccgttctaaa aagttcatcg gacttctatc tacctgtttt gatctattag
      481 ttaacgtctg catttctatt aattttagct tttagcaatg gctaatgcta aggtttgcaa
      541 atatattttt tgtacccaac taaacaattt aaatactctc aactgttttg ttttatgttt
      601 cattcttctg gttcttttgt catctattgt tgcgtcgcac tcttcaactt tttgtactta
      661 atgcatcttt attttaaaac tactttgttt taagtttcgt tttctattat taaaacttcc
      721 aaccattgtg ttttaataat actataaaag cagtaactag catattaaat aaagccaatg
      781 gcaccatttt tttccaacct aaatccatta attgatcata tctaaatctt ggtattgtcc
      841 aacgtatcca cataaataca aaaataaaaa tcactatttt tatcagtata gatgttactt
      901 gtagcaaggc tgcccagttg ccaatacttg catataaatt agcatcgttt acaaatggaa
      961 tatcatatcc accaaaatat agggttgcca ttactgcact gctcattatc atattaatat
     1021 attcagcaaa taaataaaaa cccaatttca tactagagta ctcttgatgg tagccaaaat
     1081 ttaattcgct ttctgcttca ggtaaatcaa aaggtgttct gttacactcc gcaagtgcac
     1141 aaataaaaaa taataaaaag cccaaaggtt ggtaaacaat attccataac ccttcttgtt
     1201 gctgttttac catttcgttt aatgatagag ttcctgttat cattaaaaca gcaattaagc
     1261 ttaagcccat tgccagctca taactaatag cctgagaggc tgctcttagt acagccatta
     1321 aagaaaattt attattgctt gcccagcctc caatcattat tccataaata cctaagctta
     1381 ccacagcaaa aatgtataat acagcaatat ttatatcggc aacttgtaaa tcaatttttc
     1441 tatcaaatag ttctacactt ccgccccaag gtattactgc acaagtcatt aaagaagcaa
     1501 ccattgctaa accgggacct aaaataaata accatttact tgcttgggta ggtacaattt
     1561 cttctttagt aaacattttt acaccatctg ccaaaggttg taataagcca aatggtcctg
     1621 cacggtttgg tcctggacgg tcttgtaaaa ttgcagctac tttacgttct gcaaaagtgg
     1681 tatataaagc aatgcctaaa cttgccaaaa taataatgat tattaaaaca agtttttcaa
     1741 ttataaatag ccagtcaata gttaaaaatg tcattatata taacaattaa ttattaaaaa
     1801 tctgttgaat gagctggcct gtctaaggca ctcaattcta tatttgttta gccgatgccc
     1861 cgtttttaga cgggaaatac
//
LOCUS       DAOQHP010000406         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_51310_length_1843_cov_2.960850, whole genome shotgun sequence.
ACCESSION   DAOQHP010000406 DAOQHP010000000
VERSION     DAOQHP010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51310_length_1843_cov_2.960850"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PLY81_10350"
     CDS             complement(<1..1187)
                     /locus_tag="PLY81_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNF30671.1"
                     /translation="MKFFIKQLNADAQETAGVEETTEVAATTNNLVVEETIAHDDFDW
                     SVDKRNVAVYSKEEKEKYDNVYEGTFVKIEDGEILNGDVVGLTKTDVVVNIGFKSDGL
                     ISLNEFRDVQGLKIGDSVEVMVVEKEDRNGNLHLSRKSARIFRAWERIMEVHKTGEVV
                     KGLVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKINENIKNA
                     VVSHKALIESDIEAQRAEIIGKLEKGQVLEGTVKNITDFGAFMDLGGLDGLLYITDIS
                     WGRISHPSEVLKMDQKINVVVLDFDDDKKRISLGLKQLTPHPWDVLPEGLTEGNIVKG
                     KVVNIEDYGAFLEIQPGVEGLVHVSEITWANTPINAKEFFKLNDEHEAKVVTLDKENR
                     KMS"
     gene            1355..1654
                     /locus_tag="PLY81_10355"
     CDS             1355..1654
                     /locus_tag="PLY81_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30672.1"
                     /translation="MKKTWVLFLALFLTTAVLHSQTKPKPADKKASKTVQKQAKKEVS
                     TTETKAAPASANHTKKDGTPDKRFKENKKPAAAKTKKDGTPDMRYKENKDAKRKS"
BASE COUNT          600 a          348 c          234 g          661 t
ORIGIN      
        1 aaactcattt ttctattttc tttatctaat gtaaccactt tagcttcatg ctcatcgttc
       61 agtttaaaga actcttttgc attaattgga gtattagccc aagtaatttc gctaacgtgt
      121 actaaacctt ctacacctgg ttgaatttct aagaaagcac cataatcttc aatatttacc
      181 acttttcctt ttacaatatt tccttctgtt aaaccttcag gtaaaacatc ccaagggtgt
      241 ggagttaatt gttttaaacc aaggctgata cgttttttat catcatcaaa gtctaataca
      301 accacattta ttttctgatc cattttcaat acttctgaag gatgtgaaat acgaccccaa
      361 gaaatatctg ttatgtataa taagccatct aaaccaccta aatccataaa ggcaccaaag
      421 tctgtaatat tttttacagt gccttctaat acttggcctt tttctaattt gccaattatt
      481 tctgcacgtt gtgcttcaat atcgctttct attaatgctt tgtgagatac tacggcattt
      541 ttgatgtttt cattaatttt taccacttta aattccattg ttttgccaac aaattggtca
      601 taatcggtaa ctggtttaac atcaatttga gagcctggta agaaggtttc cataccaaaa
      661 acatctacaa ttaatccgcc ttttgttttg ctggttacta agcctttaac aacttcacca
      721 gttttatgaa cttccataat acgttcccaa gcacggaaga ttcttgcact tttacggctt
      781 aaatgtaaat tgccgtttct gtcttctttc tctacaacca ttacctcaac agaatcgcca
      841 atttttaaac cttgtacatc acgaaattcg tttaaagaaa ttaatccatc gcttttaaaa
      901 ccaatattta caacaacatc ggttttagtt aagccaacca catcgccatt taaaatttcg
      961 ccatcttcaa tctttacgaa agtaccttcg tacacattat cgtacttttc tttttcttct
     1021 ttgctgtaaa cagcaacatt tcttttgtca acactccaat caaaatcgtc gtgtgcaatt
     1081 gtttcttcta ctacaagatt gtttgttgtc gcagcaactt cagtagtttc ttcaactcct
     1141 gcagtttctt gagcatctgc gtttaattgt tttataaaaa atttcataat aaaaatcccg
     1201 ttgttttttt aaacgggctg caaaggtagg tattttatat ataattaagc tattttagat
     1261 atgtttttat cataacctat tttttatcta aaatacctat tataacattt ttttaatttt
     1321 agaatgattt aaaattgttg tctaaaactt ttatatgaaa aagacatggg tattattttt
     1381 agcgttgttt ttaacaactg cagttttgca tagccaaaca aaaccaaagc cagccgataa
     1441 gaaggcttct aaaacagtac aaaaacaagc aaaaaaagaa gtttctacaa cagaaacaaa
     1501 agctgcacct gctagtgcca atcacactaa aaaagatggc actccagaca agcgttttaa
     1561 agaaaacaaa aaacctgctg cagctaaaac taaaaaagac ggcacacctg atatgcgtta
     1621 taaagaaaat aaagatgcaa agagaaaaag ctaatttttt tataaagtat attttaatag
     1681 aggctactta gttagcctct tttttttcct tatatcgtag tttaatttag ctagttggtt
     1741 tgttgcttac taaataaaaa gatacttttg cccacttata atacaaaaga atttgtttaa
     1801 acatgtattc cattcatatt aattttcaac aaaaagattt aga
//
LOCUS       DAOQHP010000407         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_51937_length_1831_cov_2.792793, whole genome shotgun sequence.
ACCESSION   DAOQHP010000407 DAOQHP010000000
VERSION     DAOQHP010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51937_length_1831_cov_2.792793"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /gene="glmS"
                     /locus_tag="PLY81_10360"
     CDS             complement(<1..1671)
                     /gene="glmS"
                     /locus_tag="PLY81_10360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNF30673.1"
                     /translation="MCGIVGYTGKRQAYPVVLKGLKRLEYRGYDSAGVALKNERGLHI
                     YKKKGKVAEMEESVVGKDVDSTIGIGHTRWATHGEPSDRNAHPHQSNDGKLAMIHNGI
                     IENYASLKQDLINKGYTFSSDTDTEVLLNFIEDIQKNNNCPLEEALRIALKRIVGAYC
                     ILLISEDDPETIIAARKGSPLVIGIGKGEHFLASDASPIIEYTKEVVYVNDYEVAIVK
                     PNELILKNLGNEKQTPFITKLDMELAAIEKGGYNHFMLKEIHEQPDTIYDCLRGRLLP
                     DTGQIMMSGIENNIDALKNAQRIVMVACGTSWHAGLIAEYMIEELCRIPVEVEYASEF
                     RYRNPIIHKGDVIVAISQSGETADTLVALERAKEQGAFIFGVVNVVGSSIARISHAGA
                     YTHAGPEIGVASTKAFTGQLAVLAMMALKIANAKQTITQDRYIQLMKDLHNIPEKVKE
                     ILADTSTIERIAEKYKNASDFLFLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALVDENLPVVFVATKDSYYEKIVSNVQEIKARKGQIIAVATNDDTV"
BASE COUNT          547 a          356 c          251 g          677 t
ORIGIN      
        1 aacagtatca tcattggttg ctactgcaat tatttgcccc tttcttgctt taatttcttg
       61 cacattgctt acaatttttt cgtaatacga atcttttgtt gcaacaaaaa ctactggcag
      121 gttttcatca accaaagcaa ttgggccatg tttcatttct gcagctgggt acccttctgc
      181 atgaatgtaa gaaatttctt taagttttaa agcaccctct aaagcaacag gaaaattata
      241 cccacgacct aaaaataaaa agtcggaagc atttttgtat ttttctgcaa tgcgttcaat
      301 agtagatgta tctgctaaaa tttcttttac tttttcagga atgttgtgta gatctttcat
      361 taattgaata tatctatcct gtgtaattgt ttgtttggca tttgcaattt ttaaagccat
      421 cattgctaaa actgctagct gaccagtaaa tgcttttgta ctggcaacgc caatttctgg
      481 gccagcatgt gtgtaagcac ctgcatggct tatacgagct atactactac caacaacatt
      541 aactacacca aaaataaatg caccttgttc ttttgctctt tctagagcta ctaaagtatc
      601 tgcagtttct ccactttgag aaatagcaac tataacatct cctttatgaa taattggatt
      661 tcggtatcta aactcacttg catattctac ttcaacaggt attctacaca attcttctat
      721 catatactct gcaataagcc ctgcatgcca actagtgcca caagccacca taacaattct
      781 ttgtgcattt tttaatgcat ctatattgtt ttcaattcca ctcatcatta tttgtcctgt
      841 atctggcaat aatctacctc ttaaacaatc ataaatagta tctggctgtt catgaatttc
      901 tttcaacata aaatggttgt aaccaccttt ttcaatggca gcaagttcca tatctaattt
      961 agtaataaaa ggagtttgtt tttcgttgcc taaatttttt agaataagtt cattgggctt
     1021 tacaatggca acttcataat catttacata cactacttct ttggtgtatt caataatagg
     1081 gcttgcatcg cttgctaaaa aatgttcacc tttaccaata ccaattacaa gagggctgcc
     1141 tttacgtgct gcaataattg tttctgggtc gtcttcgcta attaataaaa tacaatatgc
     1201 acctacaatt cttttcagtg caattcttaa agcttcttct aaaggacagt tattgttttt
     1261 ttgaatatct tcaataaagt tcaataaaac ttctgtatct gtatcgctag aaaaagtata
     1321 acctttattt attaaatctt gttttagcga agcgtaattt tcaataatgc cattatgtat
     1381 catggcaagt tttccatcat tgctttgatg ggggtgggca tttctatcac ttggttcacc
     1441 atgcgttgcc caacgagtat ggccaattcc aatagtggaa tctacatctt taccaacaac
     1501 actttcttcc atttctgcca ctttaccttt ctttttataa atatgtaatc ctctttcgtt
     1561 ttttagtgca acacctgcac tatcatatcc acgatattct aaacgtttta aaccttttaa
     1621 aactacaggg taagcttgtc ttttacctgt ataaccaaca attccacaca taagtattta
     1681 attattaatg atgttattac tttatttatt ctaaataacc tacaaattta aggtattgta
     1741 gccaaagtaa ataagttgta gattaataac ctttttagga aaattatttt gctaatttgc
     1801 caaaccattt tttagacatt gtgagtaaaa c
//
LOCUS       DAOQHP010000408         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_52038_length_1829_cov_2.601466, whole genome shotgun sequence.
ACCESSION   DAOQHP010000408 DAOQHP010000000
VERSION     DAOQHP010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52038_length_1829_cov_2.601466"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLY81_10365"
     CDS             complement(<1..454)
                     /locus_tag="PLY81_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNF30674.1"
                     /translation="MHTNTNKLAIGIDIGGTSTKFGIVNSKGEILEQNKISSTQHADI
                     NLFIAELYQVINPLIEKYGGKNAIKGIGIGAPNGNFYTGTIEYAPNLIWKEIIPLASL
                     IQKAFNLPTFLTNDANAAAFGEMKYGKAKEIKHFITITLGTGVGSGIVI"
     gene            complement(475..954)
                     /gene="rnhA"
                     /locus_tag="PLY81_10370"
     CDS             complement(475..954)
                     /gene="rnhA"
                     /locus_tag="PLY81_10370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNF30675.1"
                     /translation="MHLLKIYTDGASRGNPGKGGFGIVMLWGNTKKEIAAGFRLTTNN
                     RMELMAVIVALKQLTKKNISVTIYTDSKYVVDSIEKKWLDNWIKTDFKGGKKNKDLWL
                     EFYTLSKDFKIKMQWVKGHAENVFNNRCDELATTAADANNLLIDEGYEKTAGGYLQP"
     gene            complement(972..>1829)
                     /locus_tag="PLY81_10375"
     CDS             complement(972..>1829)
                     /locus_tag="PLY81_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNF30676.1"
                     /translation="AGLINPFLSSCINAIEPTKNSQEIYSIMPEGNAKPYDMREIIER
                     MVDASEFDEFKKDYGKTILCGYARIEGWAVGIVANQRLIVKSKKGEMQMGGVIYNDSA
                     DKAARFIMNCNQKKIPLIFLQDVTGFMVGSRSEHSGIIKDGAKLVNAVANSVVPKITI
                     IVGNSYGAGNYAMCGKAYDPRFIYAWPSAKIAVMGGEQAAKTMLQIQVATMKAKGKEL
                     TQEEETELLDKLKSKYNNQTTPYYAAARLWVDEIIDPLQTRQLISEAIHAANHQNIIP
                     EFKTGVFQV"
BASE COUNT          517 a          342 c          268 g          702 t
ORIGIN      
        1 caattacaat tccactacct acacctgttc ctaaagtgat tgtaataaaa tgtttaattt
       61 ctttagcttt gccatatttc atttctccaa aagctgctgc attagcatcg ttggttaaga
      121 aggttggtaa attaaatgct ttttgtatta agcttgctaa tggtataatt tctttccata
      181 ttaaattagg ggcatactca attgtaccag tataaaagtt gccattagga gcaccaatgc
      241 caattccttt aatagcattt tttccaccat atttttcaat taatggattt atgacttgat
      301 ataattctgc tataaataaa ttaatatcag catgctgtgt agaagatatc ttattttgct
      361 ctagaatttc acctttagaa tttacgattc caaattttgt tgaagttcct cctatatcaa
      421 ttccaatggc aagtttattt gtatttgtat gcattgtaat ttgaagaaat ttatttaagg
      481 ttgaaggtag ccacctgctg ttttttcata cccttcatct attaataaat tgttggcatc
      541 ggcagcagta gttgctaatt catcgcaacg gttgttaaaa acattttctg catgtccttt
      601 tacccattgc attttaattt taaagtcttt ggaaagagta taaaattcta gccaaaggtc
      661 tttgtttttc tttcctcctt taaaatcagt tttaatccag ttatctaacc attttttttc
      721 tatactatct accacatatt tactatctgt ataaatggta acagatatat tttttttggt
      781 taactgtttt aatgctacta taacagccat taactccatt ctgttattgg tagttaaacg
      841 aaagcctgca gcaatttctt ttttggtatt gccccatagc attacaatac caaagccacc
      901 tttgcctgga tttcctctgc tagcaccatc ggtatatatt tttaataaat gcaaaagata
      961 atttgaaaat tttacacttg aaagacaccg gttttaaatt ctggaataat attttgatgg
     1021 ttggctgcat gaatggcttc agaaattaat tgccttgttt gtaatgggtc aataatttca
     1081 tcaacccaca gccttgctgc tgcataataa ggggttgttt ggttattata tttgcttttc
     1141 agtttatcta gtaattctgt ttcttcttct tgtgttaatt ctttaccttt tgctttcatg
     1201 gtggctacct gtatttgtaa cattgttttg gctgcttgct ctccgcccat aacagcaatt
     1261 tttgcactgg gccaagcata aataaaccga ggatcgtatg ctttgccaca cattgcataa
     1321 ttgccagcac cataactgtt acccacaatt atggttattt tgggtactac agaattggca
     1381 acagcattaa caagtttagc accatctttt attataccac tatgttcact acgactgcct
     1441 accataaaac ctgtaacatc ttgtaaaaaa attaatggta ttttcttttg gttgcaattc
     1501 ataataaaac gtgcagcttt atcagcactg tcgttataaa ttacaccacc catttgcatt
     1561 tcgccttttt tgctttttac aattaaacgt tggttggcta caatgcctac tgcccatccc
     1621 tctattctgg catagccaca taaaatagtt ttgccataat cttttttaaa ttcatcaaat
     1681 tccgaggcat ctaccattcg ttcaattatc tctctcatgt cataaggttt tgcatttcct
     1741 tcgggcatga tgctataaat ttcttggcta ttttttgttg gttcaattgc attaatgcaa
     1801 gaagacaaaa aaggattaat aagtcccgc
//
LOCUS       DAOQHP010000409         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_52493_length_1820_cov_3.113314, whole genome shotgun sequence.
ACCESSION   DAOQHP010000409 DAOQHP010000000
VERSION     DAOQHP010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52493_length_1820_cov_3.113314"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..693)
                     /locus_tag="PLY81_10380"
     CDS             complement(187..693)
                     /locus_tag="PLY81_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30677.1"
                     /translation="MLYRKLFVFALLVIVNFAIAQNENNYQTPPKEISDLLLAKPTPT
                     VSIDSKGEWMLICERSSYPSVEELGEPEIRVAGLRVNPNNYAYSRQNYFTSLVLKNIK
                     TKKEYKLLGLPTNLKASNISWSPNDKKIVFTNITKHSIDAYVITVTNQTVTKINKKPL
                     NKLFNFMK"
     gene            complement(782..>1820)
                     /locus_tag="PLY81_10385"
     CDS             complement(782..>1820)
                     /locus_tag="PLY81_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF30678.1"
                     /translation="KIDSKENKIVTDIGIFEYDYLVLATGANTNFFGNELLQKHAFPM
                     KSTVEALQIRHRLIQNLEDALLLQNKHEIEKLLSIVVVGGGPTGVEVSGSLAEMKKYV
                     LPKDYPEIDFSLMKIYLLEGTDKTLASMSNKSSMHSLQYLQKLGVDVHLNSILESYDG
                     QKVKLKNGIEIPTNTVIWAAGIKGNVPNGIENTIITRGNRIKVDRQNKVLHQQNIFAL
                     GDVAYMETTLYPNGHPQVANVAINQAKNLAKNILLLQKNNSNLLEFEYKDKGSMATVG
                     RNLAVVDFQKPSLHFKGFIAWYIWMGLHLFLLVGVKNRIQVFINWIYNYLTYDQNLRL
                     IFKEFYKPKST"
BASE COUNT          599 a          283 c          245 g          693 t
ORIGIN      
        1 aagaattata gctaaatatt gaattattat cgctcatttt tagttataaa agcgctattt
       61 tataaaaact caagtagtac ttaattattt tataaaaatt ataatgattt caccaatcaa
      121 tattattaaa tgaccttgct ggaacagttt tgtatttaaa gtttcaattt tgggagaaaa
      181 aatgaattac ttcataaagt taaataattt attcaaaggt tttttattaa tttttgtaac
      241 tgtttgattg gttacagtta ttacataggc atcaatacta tgttttgtaa tattggtaaa
      301 aacaatcttt ttatcatttg ggctccacga tatattacta gcttttaaat tagttggcaa
      361 ccccaataac ttatattctt tttttgtttt aatattttta agaacaaggc ttgtaaaata
      421 gttttgcctg ctataagcat aattattagg atttactctt aaccctgcta cacgtatttc
      481 tggttcacct aattcctcca cactagggta gctgctcctt tcgcaaatta gcatccattc
      541 tcctttacta tctatagata cagttggcgt aggttttgca agtaataaat ctgaaatttc
      601 ttttggaggt gtttgataat tattttcgtt ttgagctatt gcaaaattta caattactaa
      661 aagagcaaaa acaaataatt ttctataaag catagtagca atttttaggg tgtgaagata
      721 agaactgaaa aatgatttta tagaaaatct atttaaagat gcttatattg aaaaagaacg
      781 cctatgtaga tttaggtttg taaaattctt taaaaataag ccttaaattt tgatcgtagg
      841 taaggtaatt gtatatccaa ttaataaaaa cctgaattct gttttttact ccaactaata
      901 aaaacaaatg taaccccatc caaatatacc aagcaataaa tcctttaaaa tgtaaagagg
      961 gtttttgaaa atcaacaaca gctaagttcc ttcctacagt agccatacta cctttatctt
     1021 tatattcaaa ttctagtaaa ttagaattat ttttttgaag taataaaata ttttttgcta
     1081 aatttttagc ttggttaatg gcaacatttg ctacttgagg atgaccatta gggtacagag
     1141 tagtttccat ataagcaaca tctcctaaag caaaaatatt ttgttgatgt aagactttgt
     1201 tttgcctgtc aacttttatt ctattgcctc ttgttataat ggtgttttct attccgttag
     1261 gtacattacc tttaatacca gcagcccaaa taactgtatt ggtaggtatt tcaataccat
     1321 ttttaagttt tactttttgc ccatcgtagc tttctaatat agagtttaaa tgcacatcca
     1381 cacctaactt ttgtagatac tgtaaggagt gcatactgct tttattgctc atagatgcta
     1441 gtgttttatc tgtgccttcc agcaaataaa ttttcattaa ggagaaatct atttctggat
     1501 aatctttagg taaaacatac tttttcatct ctgctaaaga gccactaact tcaactcctg
     1561 ttgggcctcc accaaccaca acaatagata ataatttttc aatttcatgt ttattttgca
     1621 acaacaatgc atcttctaaa ttctgtataa gcctatgcct tatttgcaaa gcctccacag
     1681 tgcttttcat tgggaaagca tgcttctgta aaagttcatt tccaaaaaag ttggtatttg
     1741 caccagttgc aagtactaaa taatcatact caaaaatgcc aatatctgtt actattttat
     1801 tttctttaga gtcaattttt
//
LOCUS       DAOQHP010000410         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_52652_length_1817_cov_4.187855, whole genome shotgun sequence.
ACCESSION   DAOQHP010000410 DAOQHP010000000
VERSION     DAOQHP010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52652_length_1817_cov_4.187855"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1817
                     /locus_tag="PLY81_10390"
     CDS             <1..>1817
                     /locus_tag="PLY81_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30679.1"
                     /translation="YSIQLYDTATTYFLKSIQFFYKHPNNNYESLYNISLNIIECCIQ
                     TANYNTLLLTANQALPFVEANNSKESEAYYTMFYYKGFALYNLEQYDKAEYIYKNILP
                     IAEKLYGKNNSEYITVLKYYFRILSKLEKWNKAEETLSEILQIQERIGYEDVQDVAEL
                     YEEAGNFYASIGNYSKAENCFKLALNTLENAGKKNSDLYYSILHSKAYVIYQAGKLIE
                     TKDILENLLKVYTKKNGRVNATNAEILIVLAGAEIQLNYLAAAKEHLDEGYNMTIKVH
                     GKEYPTIANAKESYGLWYNKTGDSKKSIENLKDGIQLLEKIQTTNYNIQYTRLQASLH
                     SNIGITYLEMGKYADAENHLTQSLQIREKILGIKHPEYALSLINLGVVHLYQARYEEA
                     DKMLLAALKIYLDNNLTNTVNFYYLINNIALLAEKTNGANQAKVLYQNILTILEKNNS
                     TNSAVYSIIYGNLATLSFNKEMYEDALHYCTKAIHYLEQGNNQNSKEYIKILNTQFLA
                     YKNKKEFGMAAKMSNPLIELTKKIMGNDAELTGIVYNNIAMLYEELNDVEKAADFLNK
                     GNGILLNNFKQNFYILSEKEKLTWWNNQSFIFNIFPSML"
BASE COUNT          747 a          229 c          265 g          576 t
ORIGIN      
        1 tttattctat tcaattatat gatacagcta ctacttattt tttaaagagt attcaatttt
       61 tttataaaca ccccaataat aattatgaaa gcctttacaa tattagttta aacattatag
      121 aatgctgcat acaaacagct aactataata ctttattact tactgccaat caagctctac
      181 cttttgttga agccaataat tctaaagaat cggaagctta ttatactatg ttttattata
      241 aaggctttgc cttatataat ttagaacagt atgataaggc agaatacatt tataaaaata
      301 ttttgcctat agcagaaaaa ttgtatggaa aaaacaattc ggaatatata acagttttaa
      361 agtactattt tagaatatta agcaaattag aaaaatggaa taaagccgaa gaaaccttaa
      421 gtgaaatatt acaaattcag gaaagaattg ggtatgaaga tgtgcaagat gtagctgaac
      481 tatatgaaga ggcaggtaat ttttatgcat ccataggcaa ttattcaaaa gcagaaaact
      541 gttttaaatt agcattaaat acattggaaa atgcaggaaa gaaaaacagt gatttatatt
      601 actctatact acacagtaaa gcttatgtta tttatcaagc tggtaaacta attgaaacta
      661 aagatatact tgaaaacttg ttaaaagttt atactaaaaa aaatggcaga gtaaatgcaa
      721 caaatgcaga aattttaatt gttttagcag gtgccgaaat acaattaaat tatttggcag
      781 ccgccaaaga acatttagat gaaggctata atatgactat aaaagtacat ggtaaagaat
      841 atccaaccat tgcaaatgca aaagaaagtt acgggctatg gtacaacaaa acaggtgata
      901 gtaaaaaaag tatagagaat ttaaaagatg gtattcaact tctagaaaaa attcaaacaa
      961 ccaattacaa tattcaatat actagactac aagcatcttt acactctaat attggtataa
     1021 catatttaga aatgggtaaa tatgcagatg ctgaaaatca tttaacccaa tcgttacaaa
     1081 ttagagaaaa gattttaggt attaaacatc cagaatatgc attaagttta ataaacttag
     1141 gcgttgttca cttatatcag gcacggtacg aagaagcaga taaaatgtta ctggctgctc
     1201 ttaaaattta tttagataat aacttaacaa atactgtaaa tttttattac ttaatcaaca
     1261 atattgcatt attggcagaa aaaacaaatg gtgccaatca agctaaagtt ttatatcaaa
     1321 acatacttac aattctggag aaaaacaaca gtacaaattc tgctgtttat agtattatat
     1381 atggcaattt ggcaacctta agttttaata aggaaatgta tgaagatgct ttacattatt
     1441 gtactaaagc tattcattat ttagagcaag gtaataacca aaattcaaaa gagtatataa
     1501 aaatattaaa tacccaattt ttagcatata aaaataaaaa agaatttggc atggctgcta
     1561 aaatgagtaa cccacttata gagttaacta aaaaaattat gggtaatgat gcagagctaa
     1621 ctggcattgt ttataataat attgctatgc tgtacgaaga actaaatgat gttgaaaaag
     1681 cagccgattt tttaaacaaa ggcaatggta ttttattgaa taattttaag caaaattttt
     1741 atatacttag cgaaaaagaa aagcttacct ggtggaataa tcagtctttt atttttaata
     1801 tttttccatc tatgtta
//
LOCUS       DAOQHP010000411         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_53083_length_1809_cov_2.980616, whole genome shotgun sequence.
ACCESSION   DAOQHP010000411 DAOQHP010000000
VERSION     DAOQHP010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53083_length_1809_cov_2.980616"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..959
                     /locus_tag="PLY81_10395"
     CDS             78..959
                     /locus_tag="PLY81_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30680.1"
                     /translation="MARSIQTIIDGMDAEQAAQTDLSGLNSTSNSAIYTLWKYIVATQ
                     MYLQEVLWDIFKKDLETIVDKAPVGSDKWLHDRVLKFQYSAITPQKVEVLDDFSVGYA
                     TEDTTLRIITRAAVRTVATRTVNVKVAKEEPPVALTPTELAALQSYLNNAGDGTYAGR
                     GVGIGFSGVRYVASSLDADKIYLGATIYYNGQYTNVISDNVIAAINTYLANIQSDGTF
                     KLTSLVDAIQSVTGVTDVVLENVAIRPDTEVIANSTYLVQAKTTIIPTYQLTAGYVVE
                     EDTTNYLFTDTLTFVAQ"
     gene            959..1633
                     /locus_tag="PLY81_10400"
     CDS             959..1633
                     /locus_tag="PLY81_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30681.1"
                     /translation="MADIYDYDNELVGEQLTPPVLRKTKFLAWLNVITSPVQKLWNTI
                     FIDYKDGSDYPDYDAFTLYALGDRVIYTDKMIYEAIDASLGALPSDTTKWVLINDNFI
                     GVKERIKYNSQIILLEYALNKWYRIPSTDPQIYIENTSNVSMQFVLGDSSATSSLMPN
                     DSINQIQYMGNNPTYPLVEYDFIVWVPTAVYNTLGNDTANRYNNVYNFVSKYVLSGIK
                     FRIGDY"
BASE COUNT          677 a          232 c          299 g          601 t
ORIGIN      
        1 aaccaaacac aagatactac aaccgctact ttaacaccga ttattaatgc aacaattaat
       61 acaatagacg aaatttaatg gcaaggtcaa tacaaactat aatagatgga atggatgcag
      121 aacaagctgc acaaacagat ttaagtggtt taaattctac ttctaattct gcaatttaca
      181 cactatggaa atacattgta gcaacacaaa tgtatttaca agaagtatta tgggatatat
      241 ttaaaaaaga cttagaaact attgtagata aagcacctgt aggaagtgat aaatggttac
      301 acgatagggt attaaaattt caatattcag ccataacacc tcagaaagtt gaggttttag
      361 atgatttttc tgtaggatat gcaactgaag atactacatt aagaattatt acaagagcag
      421 cagttagaac ggttgcaact agaacagtaa atgttaaagt agcaaaagaa gagccacctg
      481 tagcattaac tcctactgaa ttagcagcat tacaaagcta cttaaataat gcaggagatg
      541 gaacatacgc tggtcgtggt gttggtattg gattttcagg tgttagatat gttgctagtt
      601 cattagatgc tgataaaata tatttaggag ctactattta ctataacggt caatatacaa
      661 atgttattag tgataatgtt atagcagcta ttaacacata cttagcaaat atacaatctg
      721 atggaacttt taaattaaca tctttagtag atgctattca aagtgttaca ggtgttacag
      781 atgttgtatt agaaaacgta gcaattagac cagatacaga agttattgct aatagtactt
      841 atttagtaca agctaaaaca actattatac ctacatatca attaacagca ggttatgtag
      901 tagaagaaga tacaactaac tatttattta cagatacatt aacttttgta gctcaataat
      961 ggcagatatt tacgattatg ataatgagtt agtaggtgaa caattaacac cacctgtgct
     1021 aagaaaaact aagtttttag catggttaaa tgttattact agccctgttc aaaagttatg
     1081 gaatactatt tttattgatt ataaagatgg ttctgattat cctgattatg atgcttttac
     1141 tttatatgct ttaggtgata gggttatata tacggataaa atgatatatg aagctattga
     1201 tgcttcttta ggtgctttac ctagtgatac tacaaaatgg gtattaatta acgataattt
     1261 tataggagtt aaagaacgta taaaatataa ctcacaaatt attttattag aatatgcttt
     1321 aaataagtgg tacagaatac catctacaga ccctcaaatt tatattgaaa atacttctaa
     1381 tgtatcaatg cagtttgttt taggtgattc aagtgcaaca agtagtttaa tgccaaatga
     1441 tagtattaat caaattcaat acatgggtaa taaccctact tatccacttg ttgaatatga
     1501 ttttattgtt tgggttccta ctgctgttta taatacttta ggaaatgata ctgcaaatag
     1561 atataataat gtttataact ttgttagtaa atatgtatta agtggaatta aatttagaat
     1621 aggagattat taattatgaa aaaaataagt acaacacaaa ttgatgatgc aaatgataaa
     1681 ctacaaccgt ttacaggtca ttcattagaa tttttacaag atgctttaga tgaagataaa
     1741 gctgctatta ttaaagcatt agttacaagt aatttgggta gttattcatt agcagtacct
     1801 tatgttatt
//
LOCUS       DAOQHP010000412         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_53867_length_1795_cov_2.911494, whole genome shotgun sequence.
ACCESSION   DAOQHP010000412 DAOQHP010000000
VERSION     DAOQHP010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53867_length_1795_cov_2.911494"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1682
                     /locus_tag="PLY81_10405"
     CDS             24..1682
                     /locus_tag="PLY81_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30682.1"
                     /translation="MKKIATIIFTLMLVWSGINAQSIEDGKKLLSYQKNKSAVDLFQQ
                     LYNKNTNSAEHIYWYGQSLIANKNYAEAKALYQKALQAGVNEPLIWIGAGHIELIEKG
                     DLNSAKQKFEQAITASTPTKGRNKHKPDPSILNAIGRANAYGDSKMGDPNYGIEKLRQ
                     ASEIDLKDAEILVNMGKCYQKLGGEMGGDAVKAYTEATVRDPKYAEANWRMGLIYYSQ
                     DNKDMYEKYYNAAIASDIEFPPVYLSYYRVYEQTNVNVAKEYLDKYMQYADKDCGTDY
                     FYADYLFRAGKYQESLNKAIEMSNSNCKNFERLPILFAYNYDRLGDSINAKKFIDQFF
                     ATTNTADIEATDYELAVKVFSKFEGVENQTVAFLQKAIDNDSKKENKLKYTKMAADIY
                     GKAKMYKEHVQWLHKYNDLKGSMGEYDYYVITNSSFQSKDYVGTMVLAQKYIAAFPNK
                     PQGYAYNVAAAKALDTSSTICFAEPAILQYNDFLLKDSTTNKSQIVSNYYYIMIYYAD
                     KIKDYSKALEYCNKILLIIPDDKEMLDIKKVLEERAAKSPKSTK"
BASE COUNT          719 a          251 c          296 g          529 t
ORIGIN      
        1 ccatttaatt aaacctgaaa atgatgaaga aaattgctac aattattttt acacttatgt
       61 tggtttggag tggaataaat gctcaaagta tagaggatgg gaaaaaactt ttatcttatc
      121 aaaaaaacaa aagtgctgta gatttatttc aacagttata taataaaaat acaaatagtg
      181 cagagcatat ctattggtat ggtcaatctt taattgcaaa taaaaattat gccgaggcaa
      241 aagcacttta tcaaaaagca ttacaagcag gtgttaacga acctttaatt tggattggtg
      301 caggacatat tgaacttatt gaaaaaggcg atttgaattc tgccaaacaa aagtttgaac
      361 aagctatcac tgcatctact cctactaaag gaagaaataa acataaacca gacccttcta
      421 tattaaacgc tataggtaga gctaatgctt atggcgattc taaaatggga gacccaaatt
      481 atggaattga aaaactacgt caagcttctg aaattgattt aaaagatgca gaaatattag
      541 taaatatggg caaatgctac caaaaacttg gtggagaaat gggaggcgat gcagttaaag
      601 cttatacaga agctacagtt agagacccta aatatgctga ggcaaattgg agaatgggtt
      661 taatttatta tagccaagat aataaagata tgtatgaaaa atactacaat gctgccatag
      721 cttcagacat agaatttcca ccagtgtatt taagctatta cagagtgtat gaacaaacaa
      781 atgtaaatgt ggctaaagaa tatttagata aatatatgca gtatgcagat aaagactgtg
      841 gtaccgatta tttttatgca gattacttgt ttagagcagg taaatatcaa gaaagtttaa
      901 ataaggcaat agaaatgagt aattctaatt gcaaaaactt tgaaagatta cctatattat
      961 ttgcatataa ttatgataga ttaggcgaca gcattaatgc taagaaattt attgaccaat
     1021 tttttgcaac aacaaacaca gccgatatag aagcaaccga ttatgaatta gccgtaaaag
     1081 tatttagtaa attcgaggga gtagagaacc aaactgttgc ctttttacaa aaagcaatag
     1141 ataatgactc taaaaaagaa aataaactaa aatatacaaa aatggcagcc gatatttatg
     1201 gaaaagctaa aatgtataaa gaacatgtac aatggctgca taaatacaat gacctaaaag
     1261 gaagcatggg agagtatgat tattatgtta taaccaattc atcttttcaa tctaaagact
     1321 atgtgggtac aatggtttta gcacaaaaat atatagcagc atttcctaac aaaccacaag
     1381 gctatgctta taatgttgct gctgcaaaag ccttagatac atcttcaaca atttgctttg
     1441 ccgaacctgc aattttgcaa tacaatgatt ttttattgaa agattctacc actaataaat
     1501 ctcaaattgt tagcaactat tattacatca tgatatatta tgcagataaa ataaaagact
     1561 actcaaaagc attagaatat tgcaataaaa ttttattaat tatacccgat gataaagaaa
     1621 tgttagatat taaaaaagta ctagaagaaa gggccgcaaa atcgcctaaa tcaacaaaat
     1681 aattgaagca ataattagta aaactttgct tttttagaaa ttaaagtgaa atagctctcc
     1741 aatacttgga gagtttttct tttttaccca actaacacct atttttgtag cttgt
//
LOCUS       DAOQHP010000413         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_54521_length_1782_cov_6.135495, whole genome shotgun sequence.
ACCESSION   DAOQHP010000413 DAOQHP010000000
VERSION     DAOQHP010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54521_length_1782_cov_6.135495"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..987)
                     /locus_tag="PLY81_10410"
                     /pseudo
     CDS             complement(295..987)
                     /locus_tag="PLY81_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1000..1416)
                     /locus_tag="PLY81_10415"
     CDS             complement(1000..1416)
                     /locus_tag="PLY81_10415"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HNF30683.1"
                     /translation="MKQHNSDRYIKRFSTLEHLVTMLFCIGSNSTSLREVCTNLLGLE
                     GKLKHIKLKQSPKKSTLSDTNKRRSATVFEQIYYSLLSYYNSVLSDSQFIERFGKELS
                     IIDSTTIFLFKDILKCVGRKPVNGKKKGAIKVHLQI"
BASE COUNT          573 a          264 c          279 g          666 t
ORIGIN      
        1 tgttatcgat ttttgcattt tctatttgag ctattaatgt aaaatattta gtgctgtcaa
       61 ttttattatt atatacggtt attctattta taataggatt cttaaatatt atcaatataa
      121 aaataatggc taataacgca actaatgcat tcctttcaga ccgagtatat gcccattttc
      181 gactcaaagt ttttaattct ttatcactac tgtccgataa aagtacggaa aataaactta
      241 atttattata accaatgcat acagctttaa aaagtattaa aaaaggctag caggttagcc
      301 tgagaataat tgcatttgat tttgcgtagt attttggtag tgtttttgtg gattttgtag
      361 gaaagtccat agattgatgt agttaaatag atgagttctt attaatgagc atagattagc
      421 aaacgcccat tttcgattta ttactttacg ttggattaca gtaataagaa gattgtaaat
      481 aagggtacac caaatctgaa taatgatagc attttcatta tctcctaaga agtatttaag
      541 tggaaaattt tgtttgagtt gcttgtgcaa tagctcaatt tgccaacgtt gcttatagat
      601 tgcagcgatt tgttctggct gtagattgta aatatttgta ataaactcat atcatttgcg
      661 atgctgttcg ctccaataag ctattctgcg aagtggtaat tgattgataa tcgtgccatt
      721 ctcacggata tctaattcaa tttgttcatc tttaagtacg gcatcatcag tacaactctc
      781 taaattatat tcttctttag aaacgaatct tgcattatct tttattcttg ttacaaaagg
      841 tatttgttcc ttattaaatc tttcaaaaag ggcataatct acatatcctt tatcaaaaca
      901 gtagatagtg tcttgattaa ataaaatgtg ttgtaagaag ttgctatcat gtacggtagc
      961 atccgtaaac tttaccaatg tgggcatgtt atagtcagct catatttgca aatgcacctt
     1021 gatagcacct ttttttttac catttacagg tttacgccct acgcacttca aaatgtcttt
     1081 aaatagaaaa attgtagtag aatcgatgat ggacagctct ttgccaaaac gttctataaa
     1141 ttggctgtcc gataaaactg aattataata tgataacaaa ctgtaatata tttgttcaaa
     1201 tacagtagca cttcttcgct tattagtatc gctcagtgta ctctttttgg gtgattgttt
     1261 gagcttaata tgctttagtt tgccttctaa acctaataaa ttggtacaaa cctcacgaag
     1321 tgaagtactg ttggagccaa tgcagaaaag cattgttacc aaatgttcta aggtgctaaa
     1381 acgcttaata tatctatcgc tattatgttg tttaatagca ttatcaataa tttaattagg
     1441 aattaaagaa atcagctgtc cgagaacaga attaccaaaa ttatgcgtac ttttattcat
     1501 atagtaagtt gtgcaaaaac aaactatgta atttgagcca taatcccaaa attatggctc
     1561 acttttttta tttttatcgg acagttgtga ttctttatat aaatatataa aaaaagtccc
     1621 gcataaatac gggactttca aatattaatt tagattaatt atttaggatt aatttcaatt
     1681 cttctgtttg tttgataatc agcttctgtt ttttctggag aaactaaagg ttttgtagaa
     1741 ccatagcctt tagctgttaa tcttgattta tcaacgcctt ta
//
LOCUS       DAOQHP010000414         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_55377_length_1767_cov_3.759346, whole genome shotgun sequence.
ACCESSION   DAOQHP010000414 DAOQHP010000000
VERSION     DAOQHP010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55377_length_1767_cov_3.759346"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..189)
                     /locus_tag="PLY81_10420"
     CDS             complement(13..189)
                     /locus_tag="PLY81_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30684.1"
                     /translation="MKILLKKVLIVDKESIFNNTINDILIENGIIKTINENITETAEI
                     VFQEKNCILVKNYS"
     gene            complement(761..1096)
                     /gene="yajC"
                     /locus_tag="PLY81_10425"
     CDS             complement(761..1096)
                     /gene="yajC"
                     /locus_tag="PLY81_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNF30685.1"
                     /translation="MNFLQATFLMANPNQPGSQWTSLIMMGAIILVFWFFMIRPQAKK
                     AKEQKKFIDNLQKGDKIVTIAGIHGVINKVNDDGTINLEVNPGSYIKIERSAISLEWS
                     NAINKTAAK"
     gene            complement(1126..1560)
                     /locus_tag="PLY81_10430"
     CDS             complement(1126..1560)
                     /locus_tag="PLY81_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF30686.1"
                     /translation="MKTVLYISLLLFIFGCGSEQTLKPKSLSDLEKIMADTTNYSNIH
                     WIDSIADFGTIKEGEVIEVKFRFKNIGNKPLYITNVQAGCGCTSPDYSKEAIAPQQEG
                     WVKGIFNSLNQVGTVSKQIKVITNSKNFSEHTLYFTGTVNNN"
     gene            complement(1566..>1767)
                     /locus_tag="PLY81_10435"
     CDS             complement(1566..>1767)
                     /locus_tag="PLY81_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNF30687.1"
                     /translation="LMQMGICELLYFETIPTKVTINEYIDLAKSYSTNQSGQFVNGIL
                     DSIHKELLAEGKINKVDFKKKK"
BASE COUNT          617 a          246 c          214 g          690 t
ORIGIN      
        1 aacgagtttt ttctacgaat agttttttac taatatgcaa tttttttctt gaaaaacaat
       61 ttctgcagtt tcggtaatgt tttcattaat tgttttaata attccatttt caattaaaat
      121 atcgttaatg gtattattaa aaatactttc cttatcaact attaaaactt ttttgagaag
      181 aattttcata agtgatttac aagaaattta tgcaatatta atctaaatta aattaagtgt
      241 ttagatttaa gagtaatgaa ttttaatcac tttttcaacc tttaattgta gaaatgaata
      301 gtaaaaaata taaaattaat aattaattaa taagctatga accgaatatg ggttgtgaat
      361 tataaagaat aagctgtatt tatataaaaa ctgctactat aaagtagcag aaaataaagg
      421 taactttatt aaagttagtg gagttattaa accaccagag tttaaataga aattaaaaaa
      481 taattaatgg caataaaagc aatagaatag ctttaacagc aataattaaa ctaagccaaa
      541 taaaaaattt aataagataa ctaaataaca ttttattctt taataaaatt gctttcatag
      601 tactgttata attatttata gaacacaaat gtattttaag cgttagtata acttttataa
      661 attaaggaaa tccaattact atatcagtaa aacaaattca tgacaaaaaa aactgcctca
      721 caaattggag gcagtaatac aacttttttt atttaataaa ttatttggct gcagttttgt
      781 ttattgcatt actccactct aaacttattg cacttctttc aattttaata taactaccag
      841 ggtttacttc caagttaata gtgccatcat catttacttt atttattaca ccatgtatgc
      901 ctgcaatggt tactatttta tcgccttttt gtaagttatc aataaacttt ttttgttctt
      961 tggctttttt tgcttgtgga cgaatcatga aaaaccaaaa tactaaaatt atggctccca
     1021 tcattattaa agaagtccat tggctgcctg gctgattagg attagccatt aaaaatgttg
     1081 cttgtaaaaa attcatactg ttttgttttt taagttgaaa aattattaat tattatttac
     1141 tgttcctgta aaatagagtg tatgttctga aaagtttttg gagttggtta tcactttaat
     1201 ttgtttactt acagtgccaa cttggtttaa gctattaaaa attcccttta cccaaccttc
     1261 ttgttgtggt gcaattgctt cttttgaata atctggtgag gtgcaaccac aacctgcctg
     1321 aacattggtt atatacaaag gcttgttacc aatattttta aatctaaatt tcacttctat
     1381 cacttctcct tcttttatgg taccaaaatc tgctatacta tctatccaat gtatgtttga
     1441 atagttagta gtatcggcca taattttttc taaatctgat aagcttttag gctttaaagt
     1501 ttgctctgac ccgcaaccaa aaataaaaag tagtaaactt atgtacaata ctgttttcat
     1561 taattttatt tcttcttttt aaaatcaact ttatttattt ttccttcagc cagtaattct
     1621 ttatgaatac tatctaaaat tccgtttaca aattgcccac tttggttagt gctataactt
     1681 tttgccaaat caatatactc gttaatagta actttagttg gaattgtttc aaagtacaaa
     1741 agttcacata tacccatttg cattaaa
//
LOCUS       DAOQHP010000415         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_56165_length_1752_cov_5.585740, whole genome shotgun sequence.
ACCESSION   DAOQHP010000415 DAOQHP010000000
VERSION     DAOQHP010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56165_length_1752_cov_5.585740"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLY81_10440"
     CDS             complement(<1..194)
                     /locus_tag="PLY81_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNF30688.1"
                     /translation="MQFSHLHTHTQYSLLDGAASIESLYKKALRDNMPAVAITDHGNM
                     FGAFSFVAEAWKKTKVVGKT"
     gene            complement(218..1195)
                     /locus_tag="PLY81_10445"
     CDS             complement(218..1195)
                     /locus_tag="PLY81_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNF30689.1"
                     /translation="MNLEGMSISEFDRATRINTRVFKFKGTKALGWDYKKHTTMDSFE
                     EKPVEACFSNNNQILWVSLHNAKGIVPINFSKDSIHHDLNLKKKKIYIQYPDKKRKDS
                     LEVPLIKTGKTPKVIACTSNDSFLLVSNWHSNNVSVVKLNNGVYPYGKVIATIPVQSI
                     PRGIAIDNDNQKTYIAIMGSKLISVIDNKTWLFERNIKVASKPRHIVLDNKKRLFVSY
                     NSLDKIACIDALSGKKLFDIKTKNQPRTIALSKNNKYLFVTCYGGNALEVFKINDTGF
                     IKLYTLESEGKPVGVDIYENENTIEAWVCNYKEGNVKIFTFKKEEILKK"
     gene            1209..1436
                     /locus_tag="PLY81_10450"
     CDS             1209..1436
                     /locus_tag="PLY81_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30690.1"
                     /translation="MPFVLNTTDFVPAAVLIVSAFINMSFDLTIGATVSFFSSCFALV
                     FVLYIQINPIKMIGLRLLYSVENETIVALLT"
BASE COUNT          549 a          259 c          220 g          724 t
ORIGIN      
        1 tctgttttgc ctacaacttt tgtttttttc catgcttctg ctacaaaaga aaatgcacca
       61 aacatattgc catgatctgt tattgcaact gcaggcatat tatctcttag tgctttttta
      121 tacaagcttt caatagaggc agcaccatct aacaaagaat attgtgtgtg tgtatgtaag
      181 tgtgaaaatt gcatttttat aatataattc tccttaatta tttttttaaa atttcttctt
      241 ttttaaaagt gaatattttt acgtttcctt ctttgtaatt acatacccaa gcttctattg
      301 tgttttcatt ttcataaata tctactccaa caggtttgcc ttcactttct aaagtatata
      361 attttataaa gcctgtatcg tttattttaa aaacttctaa tgcattacca ccataacagg
      421 taacaaacaa atatttattg tttttactta atgcaattgt tcttggttga tttttagttt
      481 ttatatcaaa aagtttttta ccacttaatg catctataca tgcaatttta tctagactgt
      541 tatagctaac aaaaagtctt tttttattgt ctaatacaat atgccttggt ttagaagcaa
      601 ccttaatatt tcgttcaaat aaccaggttt tattatctat aacacttatt aacttactcc
      661 ccattatagc aatatacgtt ttttggttat cattatctat agcaatacct cttggaattg
      721 attgtacagg aattgtagct attaccttac cataaggata aacaccattg tttaatttta
      781 ctacactaac attgttgcta tgccaattac taaccaataa aaaactatca ttactggtac
      841 aagcaattac cttgggtgtt ttaccagttt taattagtgg aacttccaaa ctatctttcc
      901 tttttttatc tggatattgt atataaattt ttttcttttt taaattcaaa tcatggtgaa
      961 tagaatcttt tgaaaaattt ataggaacaa ttcctttagc attatgtaag gatacccata
     1021 gaatttgatt attgttacta aaacatgctt ctaccggttt ttcttcaaag gaatccattg
     1081 tagtatgttt tttgtaatcc cagcccaagg cttttgttcc tttaaactta aatactcttg
     1141 tattaattct tgttgctctg tcaaattcag aaatactcat accttctaaa ttcattgcat
     1201 aaagtttagt gccatttgta ttgaatacaa ctgattttgt acctgctgct gttttaattg
     1261 tttctgcttt tataaatatg agttttgatt taactattgg tgcaactgta tcctttttct
     1321 ctagttgttt tgctttagtt tttgtgctat atatacaaat aaatccaatt aaaatgattg
     1381 gtttaaggtt attatatagt gtagaaaatg aaaccattgt agctctgtta acttaatttt
     1441 caaatatcgt ttttaattaa gatggttaaa aatatcaatt atccacaata aggttaaact
     1501 gaaaagaatg ttaaaaagtt ttgcagaatg atgttttttt aggtaatgaa gggttaattt
     1561 gcatcaatcg tgtactaatt atttgctttt tgtatttaat tattaaatta aataagttgt
     1621 aattatattt tttacaccat gtaggaaaca aaagaggata catgaagata caaaacacat
     1681 tttatatatt ggcagcattt gccattttaa ccagttgtaa atctttgcat actttaactg
     1741 ctaaagatgt tt
//
LOCUS       DAOQHP010000416         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_56167_length_1752_cov_4.696523, whole genome shotgun sequence.
ACCESSION   DAOQHP010000416 DAOQHP010000000
VERSION     DAOQHP010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56167_length_1752_cov_4.696523"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLY81_10455"
     CDS             complement(<1..1223)
                     /locus_tag="PLY81_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30691.1"
                     /translation="MIKSIVITLVNLLFINSLIAQSKIYGTVKGNKNKNLIAASITIV
                     GTYDGTITDSLGKYKITTYEKGQFTVEVKCLGYKTTTQPLYLDNKNVEINFILKEEIN
                     ELSAVTVTAGSFEAGDKKRTATVLTALDVYTTGGANGDITSAIKTLPGTQQVGEQEGL
                     FVRGGAGYETKQMIDGLVVNNPFFSGAPDIATRGRFSPSLFKGNVFSTGGYSALYGQA
                     LSSVLLLESIDMPERSEASASITSVFAGAGFQHLAKNKKSSIGINYGYTNLQPYFSVV
                     KQKQTFSIFPTYQNVEANFRIKTKRNGMIKFYSSFSDGKMGLRTIDIDSVTLKNQFEL
                     RNNNWYNIITYKENLGKGWKLNTGISFSTNTDKINNKVIDNNNQPTITGKIYIDSKNF
                     ILRTKQELTVLRAVA"
     gene            1410..1748
                     /locus_tag="PLY81_10460"
     CDS             1410..1748
                     /locus_tag="PLY81_10460"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30692.1"
                     /translation="MSEYVTQETFDKMKETLQKMKAIDRPAASKAIAEAREKGDLKEN
                     AEYDSAKEAQGLLEARIKQLEGAIATAKIVDTTSIDTSRVSILTKVTITNVATKKTGT
                     YQIVGEKKPI"
BASE COUNT          586 a          272 c          248 g          646 t
ORIGIN      
        1 tctgcaacag ctcttaatac tgtaagttct tgttttgttc ttaaaataaa attttttgaa
       61 tcaatataaa ttttacctgt aattgttggc tgattattgt tgtctattac tttattgttt
      121 attttatcgg tattagtgct aaagctaatt cctgtattca gtttccaacc tttacctaaa
      181 ttttctttat aagttataat attataccag ttattatttc ttaattcaaa ttgattttta
      241 agagttacac tatcaatatc aattgttctt aaacccattt tgccgtcaga aaaacttgaa
      301 taaaacttaa tcattccatt tctctttgtt ttaattctaa aatttgcttc tacattttga
      361 taagttggaa aaatactaaa ggtttgtttt tgcttaacta ccgaaaagta aggctgtagg
      421 ttagtataac cataatttat tcctatggaa gattttttgt ttttggctaa atgttgaaaa
      481 ccggcacctg caaaaactga tgtgatagat gcactagctt ctgaacgttc aggcatgtca
      541 atactttcta ataataaaac actgcttagt gcttgcccat aaagagcact atagccacct
      601 gtgctaaata cattgccttt aaataatgat ggagaaaatc taccacgtgt tgctatatct
      661 ggtgcaccag aaaaaaacgg attattaaca actaaaccat caatcatttg tttagtttcg
      721 tagcctgcac caccacgtac aaacaagcct tcttgttctc caacttgttg agtgcctggc
      781 aaagttttta ttgcactagt aatgtctcca tttgcaccac ctgttgtata aacatctaaa
      841 gcagttaata cggtagcagt tcttttttta tcaccagctt caaaactgcc tgcagttact
      901 gtaactgcac tcaattcatt aatttcttct tttaaaataa agtttatttc aacatttttg
      961 ttatctaagt ataatggttg agtggtggtt ttataaccta aacattttac ctcaacagta
     1021 aattgtccct tttcgtaagt agtaatttta tatttaccta aagagtctgt tatggtacca
     1081 tcataagtac caacaatggt aatgcttgct gcaattaaat tcttgttctt attacctttt
     1141 actgttccat aaatttttga ttgagcaatt aatgaattaa tgaataataa attaactagt
     1201 gttataacta tactttttat cattttactt cttttgatag cacaaacgta aagtagttta
     1261 taatataaac caaattattt tttattaatt aaaaaatttt gttctataaa ttaattcatt
     1321 tttctttata tttgtagttc atgcaacgct tcatgtatta aagtatatgt agcgttgtat
     1381 tttttttata agctgtaaat atttgtacaa tgagtgagta tgtaacacaa gaaacatttg
     1441 ataaaatgaa ggaaaccctt caaaaaatga aagctattga ccgccctgct gcttcaaaag
     1501 caattgcaga agcaagagaa aaaggcgatt taaaagaaaa tgctgaatat gatagtgcta
     1561 aagaggcaca aggtttattg gaagctagaa ttaaacaatt agaaggtgcc attgcaactg
     1621 ccaaaattgt tgatacaacc tctattgata ctagtagagt aagcatttta actaaagtaa
     1681 ctataactaa tgtagctact aaaaaaacag gtacttatca aattgttggt gaaaagaagc
     1741 cgatttaaaa at
//
LOCUS       DAOQHP010000417         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_56171_length_1752_cov_4.080141, whole genome shotgun sequence.
ACCESSION   DAOQHP010000417 DAOQHP010000000
VERSION     DAOQHP010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56171_length_1752_cov_4.080141"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1415
                     /locus_tag="PLY81_10465"
     CDS             264..1415
                     /locus_tag="PLY81_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNF30693.1"
                     /translation="MKLQTSRIESIDVLRGIVMIIMALDHTRDFYSNILFDPLDLTKT
                     NVPLFFTRWVTHFCAPIFVFLAGTSAYLYLSKGKTKKQAATFLFTRGLWLIFVELTII
                     NFAWSFDYTFSFVGVQVIWAIGWSMIFLSLLVYLKPLYIGIIGILIIATHNSLDNIHV
                     ESFGNYKYLWAFLHEQYFFPYNNNSRFIGIMYPIIPWIGVIAAGFWFGTLYKLEFAKR
                     KNILLLLGIACLLLFVLIRYSNQYGDLNIWTKQSNWYFTILSFINCTKYPPSLLFLLI
                     TLGIAFIVLAFLENVQSKILNVVKVYGKVPFFYYILHILFIHASAKLVAIVMGIQATN
                     VFDNVNNQWGFGLTGVYVAWAVNVALLYIPCLWFMKVKQKRKDWWLSYL"
     gene            complement(1417..>1752)
                     /locus_tag="PLY81_10470"
     CDS             complement(1417..>1752)
                     /locus_tag="PLY81_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF30694.1"
                     /translation="FKFYMNALYGSNLPYNIPGSVRYRNALRIEPYIRVDIGISALLL
                     QPEKQNRRSYSPFKNFESIWVSLEAFNLIDRANTISYLLIKDYQNNTFAMPNRLTPRL
                     INLKLIAKW"
BASE COUNT          592 a          226 c          260 g          674 t
ORIGIN      
        1 gctgtaagca aaacagatat gttagatgaa gaactaaaaa atgaaattag taaagaactg
       61 cctaacaata ttccacatat ttttatttca tctataatgc aacaggggtt aactgaatta
      121 aaagatgcac tatggaaaat attaaataaa cctgctacgc tttaattgta attacaaacc
      181 attgctaatt ttagtttaaa taaaaatttt acatttttat tgaagagtac ttacttttag
      241 taattgcttt aaaactaaaa ccaatgaaac tgcaaacaag tagaatagaa tctattgatg
      301 ttttacgtgg tattgtaatg ataataatgg cattagacca tacaagagat ttttatagca
      361 atattttatt tgacccttta gatttaacta aaacaaatgt tccattattt tttactcgtt
      421 gggtaactca tttttgtgca cccatttttg tttttttagc tggtacaagt gcttatttat
      481 atttaagtaa aggcaaaaca aaaaaacaag cagcaacttt tttatttaca agaggtttgt
      541 ggttaatttt tgtagaatta acaatcatca attttgcctg gagttttgat tacacttttt
      601 cttttgttgg tgtacaagtt atttgggcaa ttggttggag tatgattttt ttatctttat
      661 tggtttattt aaagccattg tatattggca taattggtat tttaattatt gcaacacaca
      721 atagtttaga caatattcat gtagaaagct ttggaaatta taaataccta tgggcttttt
      781 tgcatgaaca atatttcttt ccctataata ataattccag atttattgga ataatgtatc
      841 caataattcc gtggattggt gttattgctg caggcttttg gtttggtact ttatataaat
      901 tagaatttgc caaaagaaaa aatattttat tattattagg aattgcatgt ttgctattat
      961 tcgttttaat aagatacagc aaccaatatg gagatttaaa tatttggaca aaacaaagca
     1021 actggtattt taccatttta tcttttatca actgtactaa atatcctcca tctcttttat
     1081 ttttattaat tactttgggt attgcattta ttgtattagc atttttagaa aatgtacaat
     1141 ctaaaatttt aaatgtagtt aaggtatatg gcaaagtgcc tttcttttac tatatactgc
     1201 atattttatt catacatgct tcggcaaaat tagtagccat tgtaatgggc atacaagcaa
     1261 caaatgtttt tgataatgta aataaccaat ggggctttgg gctaactggt gtttatgtag
     1321 cttgggcagt taatgttgct ttactataca taccatgctt atggtttatg aaggtaaaac
     1381 aaaaaagaaa agattggtgg ttgagttatt tataaactac catttagcaa ttagctttaa
     1441 gtttattaat cgtggggtaa gcctgttagg catagcaaaa gtattattct ggtaatcttt
     1501 tattaataaa taactaatgg tattagccct atcaattaaa ttaaatgctt ctaaacttac
     1561 ccaaatactt tcaaaatttt taaaagggct ataacttctt ctattctgtt tttctggttg
     1621 taataataat gcactaatac caatatctac tcttatataa ggttctattc ttaaagcatt
     1681 tctataccgt acactaccag gtatattata gggcaaattg ctgccataca aggcattcat
     1741 gtaaaattta aa
//
LOCUS       DAOQHP010000418         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_56349_length_1749_cov_4.169421, whole genome shotgun sequence.
ACCESSION   DAOQHP010000418 DAOQHP010000000
VERSION     DAOQHP010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56349_length_1749_cov_4.169421"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLY81_10475"
     CDS             complement(<1..159)
                     /locus_tag="PLY81_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF30695.1"
                     /translation="MRKSDLINNISEKTGIPKVDVLVTVETLLKEIKQSLISGENIYI
                     RGFGSFITK"
     gene            358..1440
                     /gene="mutY"
                     /locus_tag="PLY81_10480"
     CDS             358..1440
                     /gene="mutY"
                     /locus_tag="PLY81_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNF30696.1"
                     /translation="MQTSKEKYFTKQLLNWNKKFNDRKMPWKGIKDPYKIWLSEVILQ
                     QTKVEQGIHYYIKFTEKYTNIISLANAKDKEVYKLWEGLGYYNRCKNLLFTARKLRDE
                     NAGKFPTNYHEILQLKGIGVYTAAAIASFAFNLPYAVIDGNVYRILARYFGIHTPIDS
                     NEGKKEFTHLANKLLDKRKPAVYNQAIMDFGATVCKPNLPLCNNCKLKKNCIAFISNE
                     ANLLPIKNKRIVKKHRWLNYFILINNHDVWIKERTEKDIWQNLHEFYLVESEKKIKWN
                     ELNINKQLQQLNIKNYTIKNISKEFKQQLTHQSLHVQFIQILISTKPTTLKRDYWISQ
                     NKLSTVAFPKIINEFIKNKDNYLTSS"
     gene            1538..>1749
                     /gene="ssb"
                     /locus_tag="PLY81_10485"
     CDS             1538..>1749
                     /gene="ssb"
                     /locus_tag="PLY81_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF30697.1"
                     /translation="MRGVNKVMLIGNLGKDPHIDYLEGNIGVAKFPLATTETYKDRNG
                     KLVSQTEWHTVVLWRGLADLASKYLH"
BASE COUNT          732 a          220 c          244 g          553 t
ORIGIN      
        1 tttggtgata aaactaccaa aacctctaat ataaatattc tcgccagaga taaggctttg
       61 ttttatctct ttcaatagtg tttctacagt tactaacaca tctacttttg gaatacctgt
      121 tttttcagat atgttattga ttaaatctga ttttctcata agttaatttt ttcatgtgta
      181 agcaatcaaa gtctaaagta aataaaacta tttggtattt acaagtattt ttttgaaaac
      241 atgaacacat tattatacct tttttaaaga aatctaaaat actaaaccta aaaactaatt
      301 gaataattaa ataattaaag ttttatttgt ataaacagct ttgctatata cctttttatg
      361 caaacaagta aagaaaaata ttttaccaaa caattattaa attggaataa aaaatttaac
      421 gatagaaaaa tgccttggaa gggcattaaa gacccttata aaatttggct tagtgaggtt
      481 attttacaac aaacaaaggt tgaacaaggc atccattatt atataaagtt tacagaaaaa
      541 tatacaaata taatttcatt ggcaaatgca aaagataagg aagtatataa actatgggaa
      601 ggcttaggtt actataacag atgtaaaaat ttattattta ctgcaagaaa actaagagat
      661 gaaaatgcag gaaaatttcc tacaaattac catgaaattt tacaattgaa aggaattggt
      721 gtttacacag cagcagctat agcatcattt gcatttaatt taccttatgc agttattgat
      781 ggtaatgtgt atagaatatt ggctcgctat tttggaatac acactcctat tgatagtaat
      841 gaagggaaaa aagaatttac acacttagca aacaaattat tagataaacg taaacctgca
      901 gtttacaacc aagcaataat ggattttgga gcaaccgttt gtaagcctaa tttaccctta
      961 tgtaataatt gcaagcttaa aaaaaattgt attgctttta ttagtaacga ggcgaattta
     1021 ttgccaataa aaaataaaag aattgtaaaa aaacatagat ggcttaacta ctttatacta
     1081 attaataacc atgatgtttg gattaaagaa agaacagaaa aagatatatg gcaaaactta
     1141 catgaatttt atttagttga atctgagaaa aaaataaagt ggaatgaatt aaatataaat
     1201 aaacaattgc agcaactaaa cattaaaaat tatactatca aaaatatttc taaagaattt
     1261 aaacaacaac tcactcacca aagtttacat gttcaattta tacaaatatt aatttctact
     1321 aaacctacaa ctttgaaaag agattattgg atatctcaaa acaaactttc aacggtggct
     1381 ttccctaaaa taataaatga gtttataaaa aacaaagaca actaccttac tagtagctaa
     1441 gcataaaaat aaatatggat aaaaaagtgg ttcatatcgt aaaaaaaatt attttagaac
     1501 aagaaattaa tttttttata aaaaataaaa aaaagctatg agaggagtta ataaagttat
     1561 gttgatagga aacttaggta aagacccaca cattgactac ttggaaggaa atattggagt
     1621 ggcaaaattt cctttagcta caacagaaac ttataaagac agaaatggaa aattagtgag
     1681 ccaaacagaa tggcatactg ttgttttatg gcgtgggtta gccgatttag ctagtaaata
     1741 tttacacaa
//
LOCUS       DAOQHP010000419         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_57156_length_1736_cov_3.920880, whole genome shotgun sequence.
ACCESSION   DAOQHP010000419 DAOQHP010000000
VERSION     DAOQHP010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57156_length_1736_cov_3.920880"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /gene="pruA"
                     /locus_tag="PLY81_10490"
     CDS             <1..1624
                     /gene="pruA"
                     /locus_tag="PLY81_10490"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNF30698.1"
                     /translation="GTFSYPMPVNEPVLNYAPNSAEKIKLKATLAELKNTEIDVPMYI
                     GNKEVRTNRKVAIRPPHEIKHILGYFHAGEEKHVHQAIEAALAAKESWANMQWETRAA
                     IFLKAADLIATKYRSYINGTTMLGQSKNAYQAEIDSACELIDFLRFNVHFLSEIYKQQ
                     PISGPGMHNRTEWRPLEGFVLAVTPFNFTAIGGNLPTSAAMCGNVVVWKPANTQIYSA
                     QMFMKILKEAGLPDGVINLIYVDGPTIGKVCFNHHEFAGVHFTGSTGVFNNMWETIGK
                     NMAKYKSYPRIVGETGGKDFVLAHKSANADVVATAILRGAFEFQGQKCSAASRAYIPS
                     NIADEVKKKLIAGVKSMKMGTTEDFTNFINAVIDEKSFDNIKRYIDNAKKDAKTKILV
                     GGKCDKKEGYFIQPTVIETTNPKYTTMCEEIFGPVLTLYVYPANNFEKALELVDSTSP
                     YALTGAILAQDKAAIELATNKLRNAAGNFYINDKPTGAVVGQQPFGGARASGTNDKAG
                     SALNLYRWLSARTIKETYNPPTDYSYPFLQEA"
BASE COUNT          644 a          271 c          320 g          501 t
ORIGIN      
        1 tggaaccttt tcttacccaa tgcctgtaaa tgagccagtt ttaaactatg cccccaacag
       61 tgcagaaaaa ataaaactta aagccacttt agccgaatta aaaaatactg aaatagatgt
      121 tccaatgtat attggaaata aagaagtaag aaccaataga aaagtagcca ttcggccacc
      181 acatgaaata aaacacatat taggatattt tcatgcaggc gaagaaaaac atgtacacca
      241 agcaattgaa gcagcattag ctgctaaaga aagttgggca aatatgcaat gggaaaccag
      301 agcagccatt tttttgaaag ctgccgattt aattgctacc aaatatcgta gctacataaa
      361 tggcaccacc atgcttggcc aaagtaaaaa tgcctaccaa gcagaaattg atagtgcatg
      421 tgaattaata gattttttga gattcaatgt tcacttttta agtgaaattt ataagcaaca
      481 acctattagt gggcctggta tgcacaacag aactgaatgg agaccattag aaggatttgt
      541 tttagctgta acaccattta attttacagc cattggtggt aatttaccta ctagtgcagc
      601 catgtgtggc aatgttgtag tttggaagcc agccaatacg caaatttatt ctgcacaaat
      661 gtttatgaaa attttaaaag aagcaggatt accagatggt gttattaatt taatttatgt
      721 agatgggcca accattggaa aagtgtgttt taatcatcat gaatttgcag gtgtgcattt
      781 tacaggaagt acaggtgtat ttaataatat gtgggaaacc atcggaaaaa atatggcaaa
      841 atacaaatcc tatccaagaa ttgttggaga aacaggagga aaagattttg tgttggccca
      901 taaatctgcc aatgccgatg tagtggctac tgccatttta cgtggtgcat ttgaatttca
      961 ggggcaaaaa tgtagtgcag cttccagagc atatatacca agtaatattg cagatgaagt
     1021 gaaaaagaaa ttaattgcag gtgttaaaag catgaaaatg ggaacaacag aagacttcac
     1081 caattttatt aatgccgtaa tagatgagaa aagttttgat aacattaagc gatatattga
     1141 caatgctaaa aaagatgcaa aaactaaaat cttagttggc ggtaaatgcg ataaaaaaga
     1201 aggttatttc attcaaccta cagtaattga aactaccaat ccaaaatata caactatgtg
     1261 tgaagaaatt tttggccctg ttcttacact atacgtatat cctgcaaata attttgaaaa
     1321 agccttagaa ttagtagata gtacaagccc ttatgcttta acaggtgcta tactagcaca
     1381 agataaagct gctattgagc ttgcaacaaa taaattaaga aatgctgccg gaaattttta
     1441 tataaatgat aaaccaacag gtgctgttgt tggtcaacaa ccttttggtg gtgcaagagc
     1501 cagtggtact aacgataaag caggcagtgc tttaaattta tatcgttggt taagtgcaag
     1561 aacaattaaa gaaacttata acccaccaac tgattatagt tacccttttt tacaagaagc
     1621 ataaaaataa caattgttca aaaaataaag tcgtttagga tgtttatcat tttaaacgac
     1681 tttttttatc ctgaatttca aaaaaagcag tattcaaatt aactacttta ttgtat
//
LOCUS       DAOQHP010000420         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_57217_length_1735_cov_4.261905, whole genome shotgun sequence.
ACCESSION   DAOQHP010000420 DAOQHP010000000
VERSION     DAOQHP010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57217_length_1735_cov_4.261905"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLY81_10495"
     CDS             <1..699
                     /locus_tag="PLY81_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF30699.1"
                     /translation="NINETTKYRRKIGDGCGGATYSNEVEVFVYPPIEVGTITPAVQN
                     VCTNETPELLGLTQNCHYTNGNVTYQWQRATSPSGPWTNMNGATQAVLLPKASNTTAY
                     YRLVVKSSVCNAEAVSTVSVVNVNACSPKAANTATKLAATNLSTRGDMRLYPNPVTKG
                     QTVYVTFSHNSNTKNRVTATLRSIDGRTFNTIVEGSSNKGIQIKIPANIVQGTYLMQV
                     TNGEQKWIERVVIL"
     gene            839..1111
                     /locus_tag="PLY81_10500"
     CDS             839..1111
                     /locus_tag="PLY81_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30700.1"
                     /translation="MKYNQTTLNKLEDVLGESDYTVRYERGTFQSGWCVLELKKIVVL
                     NKFLNVEGRINTLLELIPQLDINYDKLTLETQKLYDQVMANNLALV"
     gene            complement(1119..>1735)
                     /locus_tag="PLY81_10505"
     CDS             complement(1119..>1735)
                     /locus_tag="PLY81_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF30701.1"
                     /translation="GYADKYTSYKDNLVKKNRNLSFNTNIWELSINGEFNFFRFQPGF
                     SEYRFTPYVSLGIGAFAYDPYAYLYGAKHYLRPLGTEGQQDKVNYPNLKPYSNMAMCF
                     PIGFGLKYSLSEKINIYGEMVYRLTNTDYIDDVSGNYAPDAFPPNPNGTPSVAYLLQD
                     RSYEYGLPIGIKGRQRGNSKQADSYITTQIGISINIGSYRCPKY"
BASE COUNT          630 a          266 c          281 g          558 t
ORIGIN      
        1 aatattaacg aaacaacaaa atatagaaga aaaattggag atggctgtgg aggtgcaact
       61 tacagtaatg aagtagaagt atttgtttac ccaccaattg aagttggtac aattacacct
      121 gctgttcaaa atgtttgtac caatgaaaca cctgaactat taggattgac tcaaaactgc
      181 cattatacta atggtaatgt aacttaccaa tggcaaagag ctacatcgcc tagtggtcct
      241 tggacaaata tgaacggagc aacacaagct gttttattac ctaaagcatc taacacaaca
      301 gcatattaca gattggtagt taaatcatct gtttgtaatg ctgaagctgt aagtacagta
      361 tctgttgtta atgtaaatgc ttgtagccct aaagctgcaa atacagcaac taaattagca
      421 gctactaact tatcaaccag aggtgatatg aggttatatc ctaaccctgt aactaaaggt
      481 caaactgtat atgttacgtt tagccataac tccaatacaa aaaacagagt tacagctact
      541 ttaagaagta ttgatggtag aacatttaat actattgttg aaggttcaag caacaaagga
      601 attcaaataa aaattcctgc taatattgtt caaggtacat acttaatgca agtaaccaat
      661 ggagaacaaa aatggataga aagagtagtg attttgtaat atttttgaga ttaattttta
      721 atccctgcat ataaaaaatg cagggatttt ttttattcaa tagtgtggat aaaacaaaca
      781 gctaatggaa aacagtttca gtaattttga aacattgaat aatttatttt aataattaat
      841 gaaatataat caaaccacat taaataagtt agaagatgtt ttaggcgaaa gcgattatac
      901 tgtaaggtac gagagaggta cttttcagag tggttggtgt gtactagagt tgaaaaaaat
      961 tgtagtacta aataaatttt taaatgttga aggaagaata aatactcttt tagaattaat
     1021 accacaatta gatattaatt acgataagtt aactttagaa actcaaaaac tttacgatca
     1081 ggtaatggca aataatttgg ctttagtgta gttaactttt aatacttagg gcacctgtaa
     1141 ctaccaatat ttatactaat tcctatttga gtagttatat aactatcagc ttgtttactg
     1201 ttacctcttt gtctgccttt aattccaatt ggtaagccat attcgtaact tctatcttgc
     1261 aataaatatg ctaccgatgg tgtaccattt ggatttggtg gaaaagcatc aggagcataa
     1321 ttcccactta catcgtctat ataatctgta ttagtaagcc tatacaccat ttctccataa
     1381 atattaattt tttcgctaag gctatatttt aaaccaaacc caattggaaa acacattgcc
     1441 atattgctat atggttttaa attaggatag ttaaccttgt cttgctgccc ttctgtacca
     1501 agtggtctta aataatgttt agcaccatac aaataggcat aagggtcata agcaaaagca
     1561 ccaatgccta atgatacata aggggtaaat ctatattctg aaaaaccagg ctgaaaacga
     1621 aagaaattaa attcgccatt aatactaagc tcccaaatat tagtgttaaa gcttaagttc
     1681 ctattttttt ttactaaatt atctttatag ctagtgtatt tatctgcata gccta
//
LOCUS       DAOQHP010000421         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_57333_length_1733_cov_3.684148, whole genome shotgun sequence.
ACCESSION   DAOQHP010000421 DAOQHP010000000
VERSION     DAOQHP010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57333_length_1733_cov_3.684148"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /gene="recN"
                     /locus_tag="PLY81_10510"
     CDS             <1..1641
                     /gene="recN"
                     /locus_tag="PLY81_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNF30702.1"
                     /translation="LHIQNYAIIDEIEINFFNELNIITGETGAGKSIIMGALNLILGE
                     RADSTYLLNQNKKCFIEGLFITEKKEEIVNFLRTNDFDILDELVLRREIATNGKSRAF
                     INDSPATLIQLKQLAALLVDLHQQFDTLELSNSHFQRNVVDALANNAQILSNYQRLFL
                     QWQSNQKKLEELILQRNNFSKEADYNKYLLQELEEINLKENEIEGIENELEILNNSTE
                     IKTALNALYFELKDNETPIIVKLKQLSSQLNSYAAYHQNIQSITDRIQTLQIELDDIA
                     NEAATLNNAINLDENKIEILNEKLSLCYKLYKKHNVQSSAGLIAIQNELEEKLKAVLN
                     IDEVIKEKEKEAKDLLSKISIMAKEISASRNAQLIPIEKKVNSLLHQIGMPNANIKIE
                     LKEVPLNYYGKDNVEFLFDANKSNKFEPINKVASGGELSRLMLCIKSLVAQSIDLPTL
                     IFDEIDSGISGEAAKQVGIIMQQLAAKRQIICITHQPQIAGKANAHFYVYKEIKNDIV
                     KTNIKLLNNDERIINIAKMLSGEKPSTAAIESAKEMMN"
BASE COUNT          736 a          221 c          260 g          516 t
ORIGIN      
        1 ttacatattc agaactatgc aatcatagac gaaattgaaa ttaatttctt caatgaacta
       61 aatattatta caggagaaac tggtgctggc aaaagtataa ttatgggtgc tttaaattta
      121 attttaggag aaagagcaga cagtacttac ttattaaacc aaaataaaaa atgttttatt
      181 gaaggccttt ttataacaga aaaaaaagaa gagatagtta attttttaag aactaacgac
      241 tttgatattt tagatgagtt agtattaaga agagaaatag caaccaacgg aaagagtaga
      301 gcttttatta acgactcacc tgctacactt attcaattaa agcagctcgc agctttattg
      361 gttgatttac accaacaatt tgatacatta gaattaagta acagccactt tcaaagaaat
      421 gtggttgatg ctttggcaaa taatgctcaa atattatcca attaccaacg cctttttttg
      481 caatggcaaa gcaatcaaaa aaaattagaa gaacttatat tgcaaagaaa taatttcagt
      541 aaagaggcag attataacaa atatttattg caagaattag aagaaatcaa tttaaaagaa
      601 aatgaaattg aaggtataga aaatgaactt gaaattttaa ataacagtac cgaaataaaa
      661 actgctttaa atgcacttta ttttgagcta aaagataacg aaactccaat tatagtaaaa
      721 cttaaacagc tttcatctca actaaactct tatgctgcat accatcaaaa tattcaatct
      781 ataacagata gaatacaaac attgcaaatt gagttagatg atattgctaa tgaagctgca
      841 acacttaata atgctattaa tttagatgaa aataaaattg aaattttaaa tgaaaaactt
      901 tcgctttgtt ataaactata caaaaaacat aatgtacaat catcagcagg tttaattgct
      961 attcaaaacg aattagaaga aaaattaaaa gcagttttaa atattgatga agtaataaaa
     1021 gaaaaagaaa aagaggctaa agatttacta tctaaaattt caataatggc aaaagagatt
     1081 tctgcttcaa gaaatgctca attaatacct attgaaaaga aagtaaattc tttgttacat
     1141 caaattggta tgccaaatgc aaacattaaa atagaattaa aagaagtacc gttaaattat
     1201 tacggaaaag ataatgttga gtttttattt gacgctaata aaagcaacaa atttgagcca
     1261 attaataaag ttgcaagtgg tggagagtta agcaggttaa tgctttgcat aaaaagctta
     1321 gtagcacaaa gtatagattt acctacttta atttttgatg aaattgactc tggaattagc
     1381 ggtgaagctg caaaacaagt tggaattatt atgcaacaac tagcagctaa aaggcaaata
     1441 atttgtatta cacatcagcc ccaaattgct ggtaaggcaa atgctcattt ttatgtttat
     1501 aaagaaatta aaaatgatat agtaaaaacc aatattaaat tattaaacaa tgatgaacgc
     1561 attataaaca ttgccaaaat gcttagtgga gaaaagccaa gtactgcagc aatagaaagt
     1621 gcaaaagaaa tgatgaacta gttttggttg aaaataagta cattttacga acaacaaatt
     1681 agtaaaacta cgaagcctaa aagtatattt tccttgctca ttttcagtaa act
//
LOCUS       DAOQHP010000422         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_57342_length_1733_cov_2.839690, whole genome shotgun sequence.
ACCESSION   DAOQHP010000422 DAOQHP010000000
VERSION     DAOQHP010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57342_length_1733_cov_2.839690"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLY81_10515"
     CDS             <1..863
                     /locus_tag="PLY81_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNF30703.1"
                     /translation="YGIGGTNSVWLPFLQSFLNIQSNDFLDGAEILLLGYFDFSELNK
                     IIDINAENKDEKVEAFRHAVNTIDIEVEQLMHVITQNNKLPIVIGGGHNNAYPCIKGA
                     AKGWHKAGITPLAQINAINLDAHSDYRPMEGRHSGNGFKYAEEDGYLEKYCVIGLHEN
                     YIQQNVWVDMVNNPFIDCITYEDIFIHEKKSFIQSVLHATAFTKETLTGIELDIDCIQ
                     NALSSALTPVGLTPTHARQYINYAALQSKPAYLHICEGATQLNDGRVDSSTGKLISYL
                     VSDFIKAILE"
     gene            978..>1733
                     /locus_tag="PLY81_10520"
     CDS             978..>1733
                     /locus_tag="PLY81_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30704.1"
                     /translation="MLKIIVAILLCFNVNNLFAQEFIVTTPKQKRNDFAKNFVRVLNN
                     AGNYFKDIKDKALKEEDTIFTEGNVFNNKIKLKGAVAARIIEDSTTYAGYFFGNYPNL
                     DAVEAAYVNLTNLIAEALMGKVIFRSVDGSNKTPWVKQTKIAYTQNSGFFMFNMFVEI
                     YQNNADSTLNLILKVKGGKPQYYYKIACNEPIKSFMFVSSLRTQVNTFQKRKFDGCLG
                     DIPMFNCIGERKSKDSLEVVYEKLGFKELPDALK"
BASE COUNT          658 a          211 c          301 g          563 t
ORIGIN      
        1 attatggcat aggtggcact aatagtgttt ggttgccatt tctacaatct tttttaaata
       61 ttcagagtaa cgatttttta gacggtgctg aaattttact tttgggctat tttgattttt
      121 ctgaacttaa taaaattata gatattaatg cagaaaataa ggatgaaaaa gtagaagctt
      181 ttaggcatgc agtaaataca attgatatag aagtagaaca attaatgcat gtaattactc
      241 aaaataataa attaccaatt gtaattggtg gtgggcataa caatgcatac ccttgtataa
      301 aaggtgctgc taaaggctgg cataaagcag gtataacacc tcttgcacaa attaatgcaa
      361 ttaatttaga tgcacattca gattacaggc caatggaagg aaggcatagt ggcaatggtt
      421 ttaaatatgc cgaagaagat ggttatttag aaaaatattg tgttattggc ctacacgaaa
      481 attatattca gcaaaatgtt tgggtggata tggtaaacaa cccttttata gattgtatta
      541 catacgaaga tatttttatt cacgaaaaaa aatcatttat acaatctgta ttacatgcaa
      601 ctgcttttac aaaagaaact ttaacaggta ttgaacttga tatagattgc atacaaaatg
      661 cattaagtag tgctttaacc cctgttggcc ttactccaac tcatgcaagg caatacatta
      721 attatgcagc attacaaagc aaaccagcct atttacatat ttgcgaaggt gctacacaat
      781 taaatgatgg aagagtagat agcagtaccg gaaaattgat tagctattta gtgagtgatt
      841 ttataaaagc aatattagaa taatagttga agcactttaa aactaaagtt gcaaacaacg
      901 aatactatag tgttaattat tttcgttata attactataa actaagctta catttatgtt
      961 gccaaatttt ttattaaatg cttaaaatta ttgttgcaat attattatgt tttaatgtaa
     1021 ataatttgtt tgcacaagag tttatagtta ctaccccaaa gcaaaaaaga aatgattttg
     1081 caaaaaattt tgtaagagta cttaataatg ctggcaatta ttttaaagat attaaagata
     1141 aagcactaaa agaagaagat actattttta ctgaaggcaa tgtgtttaat aataaaataa
     1201 agctgaaagg tgctgtagct gcaagaataa ttgaggatag tactacctat gcaggatatt
     1261 tttttggcaa ttatccaaac cttgatgctg tagaagcagc ttatgtaaac ttaactaatt
     1321 taattgccga agcattaatg ggtaaagtaa tctttagaag tgtggatggt agcaataaaa
     1381 caccttgggt taagcaaaca aaaattgctt atacacaaaa tagcggcttt tttatgttca
     1441 atatgtttgt tgaaatttat caaaacaatg cagatagcac attaaactta attttaaaag
     1501 taaaaggagg caaaccacag tattattata aaatagcctg taatgaacct atcaaaagtt
     1561 ttatgtttgt tagttcatta agaacacaag taaatacatt tcaaaaaaga aaatttgatg
     1621 gttgtttagg agatatacca atgtttaatt gcattggaga aagaaaaagt aaagattcgt
     1681 tagaagtggt gtatgaaaaa cttgggttta aggagttgcc agatgcattg aaa
//
LOCUS       DAOQHP010000423         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_59349_length_1700_cov_3.307599, whole genome shotgun sequence.
ACCESSION   DAOQHP010000423 DAOQHP010000000
VERSION     DAOQHP010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59349_length_1700_cov_3.307599"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLY81_10525"
     CDS             <1..405
                     /locus_tag="PLY81_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF30705.1"
                     /translation="LILPAITLGIRPMAIITQITRSAMLDVLNQDYIRTAYAKGLSTK
                     MVVWKHALKNALNPVITSITGWFAELLAGAFFVEYIFGWNGIGKLTVMALENLDFPVV
                     MGSVLLTASFFILVNIIADLLYSYIDPRIKLS"
     gene            complement(416..1561)
                     /locus_tag="PLY81_10530"
     CDS             complement(416..1561)
                     /locus_tag="PLY81_10530"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNF30706.1"
                     /translation="MSLNNNSTIKKVGILGGGQLGRMLLQAAANYVVETFVLENDENC
                     PSAHLCTHFIKGDIKDFETVYNFGKKVDALTIEIESVNVEALEQLEKEGVKIYPSPSV
                     IKTINNKILQKQFYQQNQIPTAEFVVTNQANEIINHVNLLPAVHKIAQGGYDGKGVQT
                     INTKEDAVKGFNAPSVLEKCITITKEIAIIIAVNDKKETAVYPLVEMIFDPYLNLLDY
                     QFSPAKLPENIVYKVEAVALSVVKNLNSPGIFAVELFVDNNNNIWVNETAPRVHNSGH
                     HTIEGNYSSQYDMLWRIILNFPLGNTSAILPSAIVNLIGSNNYNGNAIYDGLENILSM
                     DNVFVHLYGKIITKPGRKMGHVTILSSDYSDLTYKANKIKNSLKVVA"
BASE COUNT          544 a          247 c          235 g          674 t
ORIGIN      
        1 ttaatattac ctgccataac tttaggtatt cggcctatgg caattattac ccaaattact
       61 cgtagtgcaa tgttagatgt gttaaatcag gattatatta gaacagctta tgcaaaagga
      121 ctttctacta aaatggttgt ttggaaacat gctttaaaga atgcattaaa tccagtaatt
      181 acatctatta caggatggtt tgcagaattg ttagctgggg ctttttttgt tgaatatatt
      241 tttgggtgga atggaattgg aaaacttact gttatggctt tagaaaattt agactttcct
      301 gttgttatgg gcagtgtttt acttacagca tcttttttta ttttagtaaa tattattgca
      361 gatttattgt atagttatat tgatccaaga attaaactta gctaatttta gtttattagg
      421 caacaacctt taaactgttt tttattttat ttgctttgta agtaaggtca ctataatctg
      481 aacttaagat ggttacatga cccatttttc tgccaggttt tgtaattatt ttaccataca
      541 aatgaacaaa tacattatcc atacttaaaa tattttctag cccatcatat atggcattgc
      601 cattatagtt attgctgcca attaaattaa caattgctga tggcaaaatt gctgaagtgt
      661 tacctaaagg aaaatttaaa ataattcgcc aaagcatatc atattgactg ctgtaattgc
      721 cctcaatggt atgatgccca ctattatgca ctcttggtgc tgtttcattt acccaaatat
      781 tattattgtt atctacaaac aattctactg caaaaatgcc tgggctattt aagtttttaa
      841 ctacacttaa agcaacagct tctactttat aaactatatt ttctggtaat ttggctggac
      901 taaattggta atctaataag tttaaataag gatcaaaaat catttcaact aaagggtaca
      961 cagcagtttc ttttttatca ttaactgcaa taataatagc aatctcttta gttattgtaa
     1021 tacatttttc taaaacagat ggagcattaa aaccttttac tgcatcttct tttgtgttta
     1081 ttgtttgtac accttttcca tcataaccac cttgggcaat tttatgaaca gcaggtaata
     1141 aatttacatg gtttataatt tcattagctt gattggttac aacaaattca gctgttggaa
     1201 tttgattttg ttgataaaat tgtttttgta aaattttatt attaattgtt ttaattactg
     1261 atggagaagg gtaaattttt acaccctctt tttctaattg ctctaaagct tcaacattaa
     1321 ctgattctat ttctatggtt aaagcatcta ctttttttcc gaaattatat acagtttcaa
     1381 aatcttttat atctccttta ataaaatggg tacataaatg tgcagaaggg caattttcat
     1441 cattctctaa aacaaaagtt tctaccacat aatttgctgc agcttgtaac aacattcgtc
     1501 ctaactgccc accacctaat attcctactt tttttattgt tgaattgttg tttaaagaca
     1561 taaagcttat ttaaaaatat ttaatgcaca aattgaaaca tttgttttca tattaatact
     1621 ttttaaagtg gattttttaa aattttctaa aaataacatt cgttaagggt aaaattttta
     1681 ataatttttt tggtaattaa
//
LOCUS       DAOQHP010000424         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_60107_length_1688_cov_4.225352, whole genome shotgun sequence.
ACCESSION   DAOQHP010000424 DAOQHP010000000
VERSION     DAOQHP010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60107_length_1688_cov_4.225352"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLY81_10535"
     CDS             complement(<1..573)
                     /locus_tag="PLY81_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNF30707.1"
                     /translation="MDNNFSSQVKEIISFSREEALRLGNNFIGTEHLLLGLIRDGENV
                     AIKVLKSLNVDLYALRKEIELAVKDKSGNSVANINSLPLTKQAEKVIRVTVLEAKSLK
                     SPLVETEHLLLSILKNKENIATQILNQFNIDYDLFRNELGMVGIAPINPPKSEYTDND
                     DDNDFEDEKRASYQSQKGTSKTPANSKSKTP"
     gene            661..1053
                     /locus_tag="PLY81_10540"
     CDS             661..1053
                     /locus_tag="PLY81_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF30708.1"
                     /translation="MNFKLDTKEKFTSILIEEIDINANKAENLAAVCMPYLNNSVKNI
                     ILNLSNVVTIQEDATTILLKLQQIFYEKNASFVICCLQNAVENSLENYEVLEFLNVTP
                     TESEAWDIVQMEEIERELLDSEDIEFEA"
     gene            complement(1067..>1688)
                     /gene="mtgA"
                     /locus_tag="PLY81_10545"
     CDS             complement(1067..>1688)
                     /gene="mtgA"
                     /locus_tag="PLY81_10545"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNF30709.1"
                     /translation="ITKWVMPPITITQISNSFSYGLKRDYVSWNKLGYNIKLAAIASE
                     DQLFPDHNGFDWDAIEKSFKPKKGKKKKSKIPLGGGASTISQQTAKNVFLWQGGGYVR
                     KIPEFYFTQMIEWVWGKQRILEVYLNVIEMGKGIFGVEAASQYYFKKPANKLSRTEAA
                     MIIACLPNPKKFSVVPMSKRVAWRYPQILQQMNYIEDDEDLLAIIK"
BASE COUNT          545 a          267 c          234 g          642 t
ORIGIN      
        1 aggtgttttg ctttttgaat tagccggtgt ttttgaagtg cctttttgag attgataact
       61 tgctcttttt tcatcttcaa aatcattatc atcatcatta tctgtatatt cagattttgg
      121 aggattgata ggtgcaatgc ctaccatacc taattcattt ctgaataaat cataatctat
      181 gttaaattga tttaaaattt gagtggcaat attttcttta ttttttaaaa tactaagcaa
      241 caaatgttct gtttctacca aagggctttt taaagatttt gcctctaaaa ctgttactct
      301 aattactttt tcggcttgtt tggttagtgg taaactatta atattagcaa cactattgcc
      361 agatttatct tttacggcaa gttctatttc tttgcgtaag gcatataaat caacattaag
      421 gctttttaat actttgatgg ctacattttc tccatctcta attagcccta acaacaaatg
      481 ctctgtgcca ataaagttat ttcctaatct taatgcttcc tctctactga aagaaatgat
      541 ttcttttact tgtgatgaaa aattattatc cataatatgt ttggatttat acaatgataa
      601 agaatatttt tgagctaagt taatacagat ttattcacat aatgttaatg aaaaccaatt
      661 atgaatttca aacttgatac caaagaaaaa tttacgtcta ttttaataga agaaatagat
      721 ataaatgcca ataaggctga aaatttagca gcagtttgta tgccatattt aaacaatagt
      781 gtaaaaaaca ttattttaaa tttaagcaat gtggtaacca ttcaagaaga tgctactact
      841 attttattaa aactgcaaca aattttttac gaaaaaaatg caagctttgt aatttgttgt
      901 ttacaaaatg ctgttgaaaa tagtttagaa aactatgaag ttttagaatt tttaaatgtt
      961 acacctaccg aaagtgaagc atgggatatt gtacaaatgg aagaaataga aagagagtta
     1021 ttagatagtg aggatataga gtttgaagcc taatagctgt ttactattac ttaatgattg
     1081 ctaacaaatc ttcatcatct tctatataat tcatttgttg taaaatttgt gggtaacgcc
     1141 aggcaaccct tttgctcatt ggtacaacag agaacttttt tggattgggt aaacaagcaa
     1201 taatcattgc tgcttcggta cgtgataatt tatttgcagg ttttttaaaa tagtactgcg
     1261 aggcagcttc cactccaaaa attcctttac ccatttcaat tacattcaaa tacacttcta
     1321 aaattctttg cttaccccaa acccactcta tcatttgtgt aaaataaaat tctggaattt
     1381 ttcttacgta tcctccaccc tgccataaaa aaacattttt tgcagtttgc tgagaaattg
     1441 tacttgctcc tccacctaat ggaatttttg acttcttctt ttttcctttt ttaggtttaa
     1501 aacttttttc tattgcatcc caatcaaagc cattgtgatc tggaaataac tgatcttcgc
     1561 ttgcaattgc tgctaatttt atattatacc ccaatttatt ccaacttaca taatctcttt
     1621 ttaacccgta gctaaatgaa ttactaattt gtgttattgt aataggtggc attacccatt
     1681 ttgtaata
//
LOCUS       DAOQHP010000425         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_60118_length_1688_cov_3.608696, whole genome shotgun sequence.
ACCESSION   DAOQHP010000425 DAOQHP010000000
VERSION     DAOQHP010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60118_length_1688_cov_3.608696"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..853)
                     /locus_tag="PLY81_10550"
     CDS             complement(8..853)
                     /locus_tag="PLY81_10550"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNF30710.1"
                     /translation="MSVFEVYGGQKLEGAITPQGAKNEALQVISAVLLSSETVTIHNI
                     PDILDVNLLIELLHEMKVQVTKLSRHSYSFTANNLDENYLTSEEFSKKAGRLRGSVML
                     AGPMLARFKKAYIHKPGGDKIGRRRLDTHIIGFEKLGVKFDYNTNTSCFQLSTQKLTA
                     TDMLLDEPSVTGTANIIMAAVLAEGVTTIYNAACEPYIQQLCKMLINMGAKIEGVGSN
                     LLKIYGVNKLSGTEHTILPDMIETGSFIGLAAMTQSELTIKNAGVNHLGIIPEKFNNV
                     IEIRF"
     gene            1020..>1688
                     /locus_tag="PLY81_10555"
     CDS             1020..>1688
                     /locus_tag="PLY81_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNF30711.1"
                     /translation="MQKGLLIIFLLLHILLSKLHTQPVFEFRGVWVASVVNIDWPSAK
                     SLTTQQQKDEFIKLLNMHQRNGMNTMIVQIRPAADALFSSPYEPWSEFLTGTQGLAPN
                     PLYDPLEFMITETHRRRMEFHAWINPYRAVFSTQSSSVASTNITKIKPDWFVTYNHQK
                     IFNPGLPEVRNYVTKIVEDIVARYNIDAIHMDDYFYPYPDKNNNEFNDKESFEMYGNG
                     LKKED"
BASE COUNT          552 a          287 c          251 g          598 t
ORIGIN      
        1 cttgatttta aaaccttatt tcaatgacat tgttaaattt ttctggtata attcctaaat
       61 ggttaacgcc agcattttta atagttaatt cactttgtgt catagcagca aggccaataa
      121 agctgccagt ttcaatcata tctggtaaaa tggtatgctc tgttccggat aatttattta
      181 caccataaat ttttaataaa ttacttccta caccttcaat ttttgcaccc atattgatca
      241 acattttgca tagctgttgt atataaggtt cgcaagctgc attgtaaatt gttgtaacac
      301 cttctgccag cactgcagcc attataatat ttgcagttcc ggtaacactt ggctcatcta
      361 acaacatatc ggttgcagta agcttttggg tagatagttg aaagcaacta gtatttgtat
      421 tataatcaaa ctttacacct aatttttcaa atcctataat gtgtgtatcc aatcttcttc
      481 ttccaatttt atctccacca ggtttatgaa tataggcttt cttaaacctt gcaagcattg
      541 gtccagcaag cattacacta cctcgtaatc tccctgcttt tttactaaat tcttcactag
      601 ttaaataatt ttcatctaag ttatttgctg taaaagagta actatgccta cttaatttag
      661 taacctgcac cttcatttca tgcaataact ctattaaaag atttacatct aaaatatctg
      721 gaatattatg gatggttacg gtttcactgg ataataaaac tgcactaatt acttgtaggg
      781 cttcattttt ggcaccttgt ggggttattg ctccttcaag tttctgtcct ccatatactt
      841 caaaaacact catggttatt tactaaaagt tttaatatta aagtaaaagt attatgttgt
      901 aggcacaaat tataaaatta tttgtcctcc taaaaaaatt aagaagagtt gttaaatttt
      961 taattcaaat ttttatttgc ataacagtaa ttatcttcgg ctagtttaaa aataaattca
     1021 tgcaaaaagg tttgctaatc atatttctgc tattgcatat tttgctaagt aagttacata
     1081 ctcaacccgt atttgaattt agaggagttt gggtagcaag tgtagttaat atagattggc
     1141 caagtgctaa aagtcttaca actcaacaac aaaaagatga atttattaag ttgttaaata
     1201 tgcaccagcg taacggaatg aatacaatga ttgtacaaat acgtcctgct gcagatgcac
     1261 ttttttcttc tccttacgaa ccttggagtg agtttttaac aggcacgcaa ggcttagcac
     1321 caaatccttt atacgatcct ttagagttta tgattactga aacacacagg cgtagaatgg
     1381 agtttcatgc ttggataaac ccatatagag ccgtttttag tactcaatca tcttctgttg
     1441 cttcaactaa tattactaaa ataaaaccag attggtttgt tacctataat catcaaaaaa
     1501 tttttaatcc tgggctaccc gaagttagaa attatgtaac caaaattgta gaagatattg
     1561 tggcaagata caatattgat gctatacaca tggatgatta cttttatcct tatcccgata
     1621 aaaacaataa cgaatttaac gataaagaga gttttgaaat gtatggaaat ggtttaaaaa
     1681 aagaagat
//
LOCUS       DAOQHP010000426         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_60459_length_1683_cov_3.031327, whole genome shotgun sequence.
ACCESSION   DAOQHP010000426 DAOQHP010000000
VERSION     DAOQHP010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60459_length_1683_cov_3.031327"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLY81_10560"
     CDS             <1..389
                     /locus_tag="PLY81_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNF30712.1"
                     /translation="LGYDYFIEGKVIEGDKLGRTIGYPTANLQLHNEEKLVPGDGVYA
                     VEIIWNKQPLQGMLYIGSRPVVNGKRRVIEVNIFNFNETIYQENLKIIFQSYIRADIN
                     MSNFEELKRQLDVDKTSALTFFKNSK"
     gene            complement(397..>1683)
                     /locus_tag="PLY81_10565"
     CDS             complement(397..>1683)
                     /locus_tag="PLY81_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30713.1"
                     /translation="IVGINNSGVETVLMTNINTTQQDVDIQTIDANIYPFLKLKLSNK
                     DAVHLTPYQLRYWRLIADLLPDGSLASNLFFNFKDTLEAGELLTAAIVFKNISNVSFT
                     DSLSVDVLLTNANGITTSIPISKIKKPLNAGDTSLIKIPINTKDYIGKNTLKINVNPN
                     NTPPEQYLFNNFLYKDFYVKADKVEPIIDVTFDGVHIFSGDIVSAKPVIKVELNDEAK
                     FLKLDDTAGITIQLRYPDNTVKRFAYGTDTLSFTPATIGNNNIAIADFKPLLVQDGEY
                     ELMVSGKDKSGNKAGAKQYSVTFNVNNKPMISDVFNYPNPFTSSTAFVFTLTGSQVPS
                     NIRIQILTVTGKIVKEINKDELGPIRIGKNITEYKWDGTDMYGQKLANGVYLYRVITN
                     LNGNSLERFEFKDGFGDNINTQKYFKAGYGKMYLMR"
BASE COUNT          574 a          246 c          239 g          624 t
ORIGIN      
        1 tgttgggata tgattatttt attgaaggga aagtaattga aggagataaa cttggcagaa
       61 ccataggcta cccaactgct aatttacaat tacataatga agaaaaactg gtacctggag
      121 atggtgttta tgctgtagaa attatttgga ataaacaacc attacaaggt atgttatata
      181 ttggcagcag gcctgtggta aatggaaaaa gaagggtaat tgaagtaaat atttttaatt
      241 ttaatgaaac tatttatcaa gaaaatttaa aaataatatt ccaaagttat atacgtgccg
      301 atattaatat gagcaatttt gaggagctta aaagacaatt agatgtagat aaaacatctg
      361 cactaacatt ttttaaaaat agtaagtaaa aaattcttac ctcatcaaat acattttacc
      421 ataacctgct ttaaaatatt tttgggtatt tatgttatct ccaaaaccat ctttaaactc
      481 aaatctttct aaactattgc catttaaatt ggttattact cgatataggt aaacgccatt
      541 tgctaacttt tgtccataca tatcagttcc atcccatttg tattctgtaa tattttttcc
      601 tattctaatg ggtcctaatt catctttatt aatttctttt actattttac cagttactgt
      661 taaaatttga attcgtatgt tggagggtac ttgactacca gttaatgtaa acacaaatgc
      721 tgtagaagaa gtaaaaggat tgggataatt aaacacatca gaaatcattg gtttattatt
      781 tacattaaat gtaacactgt attgttttgc ccctgctttg ttgccacttt tatctttacc
      841 acttaccatt aattcatatt ctccatcttg tacaagtaat ggtttaaaat cggcaattgc
      901 tatgttatta ttgccaatgg tagctggtgt aaaacttaaa gtatctgtac cataagcaaa
      961 acgctttaca gtattatcag ggtaacgtaa ttgaatagtt atgcctgcag tatcatctaa
     1021 ttttaaaaat ttggcttcat cattaagctc tactttaatt actggtttag cagatacaat
     1081 gtctccacta aatatatgaa ctccatcaaa agtaacatct ataattggct ccactttatc
     1141 ggccttaaca taaaaatctt tatacaaaaa attattaaac aaatattgtt ctggtggtgt
     1201 attatttggg ttaacattaa ttttcaaagt gtttttccct atataatctt tagtatttat
     1261 tggtatttta attaagcttg tatctcctgc atttaatggt ttttttattt ttgatattgg
     1321 tatagaagta gtgataccat ttgcattggt taataaaaca tctacactta aactatcggt
     1381 aaagcttaca ttgctaatat ttttaaatac aatagcagca gttaataatt cacctgcttc
     1441 caaagtatct ttaaagttaa aaaataaatt acttgccaaa gagccatctg gcaacaaatc
     1501 tgcaattaat ctccaatatc ttagttgata gggcgtaaga tgtactgcat ctttattact
     1561 tagttttagt tttaaaaatg gataaatatt ggcatcaata gtttgtatat ctacatcttg
     1621 ctgtgtggta ttaatatttg tcattaatac agtttcaaca ccagaattat ttataccaac
     1681 aat
//
LOCUS       DAOQHP010000427         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_60667_length_1680_cov_2.848615, whole genome shotgun sequence.
ACCESSION   DAOQHP010000427 DAOQHP010000000
VERSION     DAOQHP010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60667_length_1680_cov_2.848615"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1680
                     /gene="sprA"
                     /locus_tag="PLY81_10570"
     CDS             <1..>1680
                     /gene="sprA"
                     /locus_tag="PLY81_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF30714.1"
                     /translation="DDFEGTRNSFDLRFPSIAWTLASTPTGKFAEASLFDSIDYNFNR
                     AKIAWYNIEPNLQDKNAGNNPLKSNLDALSDPRTRQVYTNELFPNLTTNITNVQAFTF
                     DLAYYPTERGPYNYESSPLQVTASGRLLNPKQRWGGIMRNIDQTDFEAGNVEYLEFWV
                     QDPFIKTANPTSKGKLVINLGNISEDILKDGKRFYENGLPSPNIPAVVDSSTWGKQPI
                     NPIQVTQAFSNDPNDRPYQDVGFDGLNDEGEKNKKSYYLAKLAANFSTSSPLYIQANQ
                     DPSNDNYKWYRDASYDAVGADILQRYKNFNNPQGNSPVAGTNNDLSAAATLYPDNEDL
                     NRDNTLNETEAYYEYEVDLSKNMDVGITKYITDKRSINVKYVNGTTGTENWYLFRVPI
                     KEYTSNVGGITDFKSIRFIRMYLTDFEDSVVMRFARFDLVRNQWRNFNYKLDTAGQYI
                     ALNNNTTTFNTLAVNIEENSNRTPVNYVMPPGIERVQQLSNNGVNLLQNEQAMSLRVQ
                     GLQDGDARGVIKTLNLDMRQFGKLSMFIHAESMPKFPTVNDNELNAVIRIG"
BASE COUNT          627 a          282 c          294 g          477 t
ORIGIN      
        1 tagatgattt tgaaggcaca agaaatagct ttgatttacg tttcccatct attgcatgga
       61 ccttagcctc tacaccaaca ggtaaatttg cagaagcctc tttatttgat tctattgatt
      121 ataattttaa ccgagctaaa attgcttggt ataatattga gcctaatttg caagataaaa
      181 acgctggcaa caacccttta aaatcaaatt tagatgcatt aagcgaccca cgtacaaggc
      241 aggtttacac caatgaatta tttcctaacc ttacaaccaa tattaccaac gttcaggctt
      301 ttacttttga tttggcttat tatcctaccg aacgtggccc ttacaattac gaaagcagtc
      361 cattacaagt tactgcaagt ggtagattat taaatcccaa acaacgttgg ggcggtatta
      421 tgagaaatat tgaccaaact gattttgaag caggcaatgt agaatacctg gagttttggg
      481 tacaagatcc atttataaaa acggctaacc caacttctaa aggtaaactg gtaattaact
      541 taggtaatat aagtgaagat attttaaaag atgggaaacg tttttatgaa aatgggcttc
      601 catcaccaaa tatacctgca gtagtagata gcagcacttg gggtaaacag cctatcaacc
      661 caattcaagt tacacaagca tttagcaacg accccaatga taggccttac caagacgttg
      721 gttttgatgg gttaaatgat gagggagaaa aaaataaaaa atcttattac ttagcaaaat
      781 tggcagcaaa cttttctacc agttctccat tatatattca agcaaaccaa gaccccagta
      841 acgataacta taaatggtac agagatgcaa gttacgatgc agttggtgct gatattttac
      901 aacgctataa aaattttaat aacccacaag gcaatagccc tgtagcagga acaaacaacg
      961 atttatctgc agcagcaact ttatatccag acaatgaaga tttaaacaga gacaacactt
     1021 taaacgaaac agaagcttac tatgaatacg aagtagattt atcaaaaaat atggatgtag
     1081 gcattacaaa atatattaca gataaaagaa gcattaatgt aaaatacgta aacggaacaa
     1141 caggaacaga aaattggtat ttatttagag ttcctattaa agaatatacc agtaatgtag
     1201 gtggaattac agattttaaa tccattcgtt ttataagaat gtatttaacc gattttgaag
     1261 actcggttgt aatgcgtttt gcaaggtttg atttagttag aaaccaatgg agaaatttta
     1321 attataagtt agatactgca ggccagtata ttgccctaaa caataatact acaaccttta
     1381 acactttagc tgtaaacatt gaagaaaaca gcaatcgtac ccctgtaaat tatgtaatgc
     1441 caccaggtat agaaagagta caacaattaa gcaataacgg agtaaaccta ttacaaaacg
     1501 aacaggcaat gagtttaaga gtacaaggtt tacaagatgg tgatgcaaga ggcgtaataa
     1561 aaactctgaa tttagatatg cgtcagtttg gcaaattatc aatgtttatt catgccgaga
     1621 gtatgcctaa atttccaaca gttaatgaca atgaattaaa tgcagtaatt agaattggac
//
LOCUS       DAOQHP010000428         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_61769_length_1664_cov_2.804226, whole genome shotgun sequence.
ACCESSION   DAOQHP010000428 DAOQHP010000000
VERSION     DAOQHP010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61769_length_1664_cov_2.804226"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PLY81_10575"
     CDS             complement(<1..100)
                     /locus_tag="PLY81_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNF30715.1"
                     /translation="MSNRTFTMIKPDATAKGFTGGILEMMLKAGFSI"
     gene            complement(213..761)
                     /locus_tag="PLY81_10580"
     CDS             complement(213..761)
                     /locus_tag="PLY81_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF30716.1"
                     /translation="MHFNDYLQYFETILNNTQNLQEPYNDPEFMEYTKLNWSRLNRWI
                     KTGELLDETKKTIQNIQTKQRWVLISEPWCGDAAHAVPFIVMMANLNPNITLEIQLRD
                     SGSEIEKYLTNGSKSIPLLIIRDLSGNDITKWGPRPKDCQADFSKLKAEGLEMADLKI
                     ALQKWYNNDKGVKIQQEIIALL"
     gene            complement(939..>1664)
                     /locus_tag="PLY81_10585"
     CDS             complement(939..>1664)
                     /locus_tag="PLY81_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNF30717.1"
                     /translation="MSGSLACIAGSILIVYANMGAAAGMDLAPKLITASLMAAPGALV
                     ISKIVFPETEESQTMGKVKLEVKSNYTNLIDAISHGASDGFKISMNVIAMLIGFIALI
                     TCVDYILLKTGHLFSANFDLSLNYIFGKIFYPMAWSMGVPAQDVQSAATLLGQKITVN
                     EFVAFTNLTGKTVPIVTEKGLLIVSIAICGFANFSSVGMQIGGIGEIAPNRRTDLAKL
                     GLKALLCGTLASYLSATLAGIFI"
BASE COUNT          583 a          281 c          223 g          577 t
ORIGIN      
        1 taatgctaaa accagctttt aacatcattt ctaaaatacc acctgtaaaa ccttttgcag
       61 tagcatcggg tttaatcatt gtaaaagttc tattactcat tttttttatt ttcaattgcc
      121 gcaaaagtag tgttatataa tttacaccaa aataatagtt aacagcttta agattttata
      181 aagaaaatga atagtaataa gtggtatatc atctacaata aagcaattat ttcttgctga
      241 atttttactc ctttatcatt attataccat ttttgaagag caatttttaa atcagccatt
      301 tctaaacctt cagcttttag tttactaaaa tctgcctggc agtctttagg tcttggtccc
      361 catttagtaa tatcatttcc acttaagtct cttataataa gtaatggaat agatttactg
      421 ccattggtta aatatttttc aatttcagag ccactatctc tcagttgtat ttctaaagta
      481 atattggggt ttaagtttgc catcattact ataaatggca cagcatgtgc agcatcgcca
      541 caccaaggct ctgaaattaa tacccaacgt tgtttggttt gaatattttg aattgttttt
      601 tttgtttcat ctaataattc acctgttttt atccagcggt ttaaacgaga ccaatttaac
      661 ttggtatatt ccataaactc aggatcgtta tatggttctt gcagattttg tgtattgttt
      721 aaaatggttt caaaatactg taaatagtca ttaaaatgca taattgttat tttatttata
      781 taaatcagga taattaagtc tttataaatt atttgcaaat aaatagaata aaactgaaca
      841 tacgtgtaac aaaagttaca acatattttg tcatacaaac aaaaactctc aatagtttat
      901 attgagagta gtttataaat tgaattaact taaataattt aaataaaaat tccagctaaa
      961 gttgcagaaa ggtaagaggc caaagtaccg cataacaatg cttttaagcc aagcttcgct
     1021 aaatcagttc ttctattagg tgcaatttct ccaataccac caatttgcat accaacactg
     1081 ctaaagttag caaagccaca aattgcaata cttacaatta gtaatccttt ttcggtaact
     1141 ataggaactg ttttacctgt taagttggta aaagcaacaa attcgtttac tgtaattttt
     1201 tgacctagta gggtagcagc actttgtaca tcttgtgcag gaacgcccat tgaccatgcc
     1261 ataggataaa atattttacc aaaaatataa tttaaactta agtcgaaatt agcactaaat
     1321 aaatgaccag ttttaagtaa aatataatca acacaagtaa ttaaagcaat aaaacctatt
     1381 agcattgcaa taacattcat agaaatttta aaaccatcgc tagctccatg agaaatggca
     1441 tcaattaaat tagtataatt actttttact tctaatttta ctttacccat agtttggctt
     1501 tcttctgttt cgggaaaaac aattttagaa ataaccaaag cccctggtgc agccattaaa
     1561 cttgcagtaa ttagtttagg ggctaaatcc ataccagcag cagcacccat attggcatac
     1621 acaattaata tactaccagc aatacaggca aggctaccac tcat
//
LOCUS       DAOQHP010000429         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_61901_length_1662_cov_3.075918, whole genome shotgun sequence.
ACCESSION   DAOQHP010000429 DAOQHP010000000
VERSION     DAOQHP010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61901_length_1662_cov_3.075918"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..>1662
                     /locus_tag="PLY81_10590"
     CDS             187..>1662
                     /locus_tag="PLY81_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNF30718.1"
                     /translation="MIKTNLLNSDEEVDFMPIIPIGENDSEQDANLKIPEELIILPLR
                     NTVLFPGVVLPITVGRDKSIKAVTDAYKADKLIGVLAQKDSSIEEPEAKDLCNIGTVA
                     KIVKLIKMPDGGTTIIIQGKKRFELISITKVDPYFKAKINILKEDEKPNTENFDAMVD
                     SIKDLATQIIQLSPNLPTEASIILKNIESPSFLINFVSSNQNTELAEKQALLEMNDIT
                     IRAEKLIQLLQRELQFAELKNKVTNKTRTELDKQQREYFLQQQLKSIKDELGGDHNEK
                     EVAEMKKKAETKKWANAAKELFNSNIAKLERMHPSTPDYSVIYNHCNLMLDLPWEDYT
                     ADNYDLKNAKKVLDKEHYGMYKIKNRILEYLAVLKLKGDMKSPILCFVGPPGIGKTSL
                     GKSIANAVGRKYTRVSLGGLHDESEIRGHRKTYIGAMPGRILQNLRKVKSGNPVMILD
                     EIDKVGNDHKGDPSSALLEVLDPEQNNSFYDNYLELEYDLSK"
BASE COUNT          680 a          238 c          273 g          471 t
ORIGIN      
        1 aacaatagtt aatttaaaaa aatattcaaa taacgctttt tgtcttaaaa agtagtgcaa
       61 tactaacata attatgccat tttgtacagc atttttatat aaaaaaccaa tggcattaat
      121 attgataatt gttatttctt attttaaaat taatagaaaa ttaaaaatag ctcaattaaa
      181 tatattatga taaaaactaa cttattaaat agcgacgagg aagtagattt tatgcccatt
      241 attcctattg gagagaatga ctcagaacaa gatgccaatt taaaaatacc cgaagagtta
      301 attatattgc cattaagaaa tacagtttta tttcctggag ttgttttacc cattactgta
      361 ggaagagata aaagtattaa agcagtaacc gatgcttata aagctgataa actaattggt
      421 gtacttgctc aaaaagatag cagtattgag gaaccagagg caaaagactt atgcaatatt
      481 ggtactgttg ccaaaattgt aaaattaatt aaaatgcccg atggtggaac cactattata
      541 attcaaggga aaaaaagatt tgaattaata agtattacta aagtggatcc ttattttaaa
      601 gcaaaaatta atattttaaa agaagacgaa aaaccaaata ctgaaaactt tgatgcaatg
      661 gttgattcta tcaaagattt ggcaacacaa ataattcaac tatctccaaa cctgccaact
      721 gaggctagca ttattttaaa gaatattgag agcccttctt ttctcatcaa ctttgtaagc
      781 agtaatcaaa atactgaatt agcagagaaa caggctttgt tagaaatgaa tgatattact
      841 ataagagcag agaaattaat tcagctttta caacgtgaat tacagtttgc agaattaaaa
      901 aacaaagtaa ccaacaaaac ccgaactgaa ttagacaaac aacagagaga atatttttta
      961 cagcagcaac taaaaagtat aaaagatgag ctgggcggag atcataatga aaaagaagtt
     1021 gctgaaatga agaaaaaggc cgaaacaaaa aaatgggcta atgcagctaa agaattattt
     1081 aactctaata ttgcaaagct tgaaagaatg caccccagca ctccagacta ctctgtaata
     1141 tacaatcatt gtaatttaat gttagacctg ccttgggagg attatactgc tgataattat
     1201 gatttaaaaa atgcaaaaaa ggttttagac aaagaacatt acggcatgta taaaattaaa
     1261 aacagaatac tagaatacct tgctgtatta aaattaaagg gagacatgaa aagccctatt
     1321 ctttgttttg taggcccccc aggtattggt aaaacatcct taggtaaaag tatagccaat
     1381 gctgttggta gaaaatatac ccgagttagc ttaggtggct tacatgatga aagtgaaatt
     1441 agagggcacc gaaaaacata tataggagcc atgcctggaa gaattttaca aaacctacga
     1501 aaagtaaaat ctggcaatcc tgtaatgata ttagatgaaa tagataaagt aggcaatgac
     1561 cacaaaggag acccatcatc tgctctatta gaagttttag accccgaaca aaacaattct
     1621 ttctacgata attatttaga attagaatac gacttaagta aa
//
LOCUS       DAOQHP010000430         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_62151_length_1658_cov_4.820961, whole genome shotgun sequence.
ACCESSION   DAOQHP010000430 DAOQHP010000000
VERSION     DAOQHP010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62151_length_1658_cov_4.820961"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..964
                     /locus_tag="PLY81_10595"
     CDS             158..964
                     /locus_tag="PLY81_10595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF30719.1"
                     /translation="MTKNEQYFSINKDAWNKKTPVHVESSFYNQEAFLNGKNSLNDIE
                     LDLLGNIQDKNILHLQCHFGQDTISLSRLGAIATGVDIAENAINIAKQTANQLNLQTQ
                     FICCNIYDLSNYLNQKFDIVFTSYGTIGWLPDLDKWAKIINHFLQPNGQFIMVDFHPV
                     VWMFDNDFQQVTYNYFNTGAILENEVGTYANKKTDIQFQTASWNHSIGEILNSLISNQ
                     FSINSFDEYDYSPYNCFNNTIEVETNKFRIKHMGNKLPMLYAIKATKDKT"
     gene            1041..>1658
                     /locus_tag="PLY81_10600"
     CDS             1041..>1658
                     /locus_tag="PLY81_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNF30720.1"
                     /translation="MDIKNNILETIGNTPLIKLNKVTKDFPCTVLAKVDYFNPGNSIK
                     DRMALKMVEVAEKGGKLKPGGTIIECTSGNTGMGLALAACVKGYKCIFVTTDKQSKEK
                     ADILKAVGAEVIVCPTNVMPEDPKSYYSVAKRLATEIPNSYHFNQYDNLANRLAHYES
                     TGPEIWKQTDGKITHLVCTAGTGGTITGTAKYLKEMNPNIQIWAID"
BASE COUNT          650 a          222 c          260 g          526 t
ORIGIN      
        1 gttgtttcta aatcaataaa cgcaattgga ttaattaatt gaagtttcat aaatttattt
       61 ttctaaagtg gcgaaaataa aatagaattt ataaataatg ttgctttatt tttaatatgg
      121 ttcttaattt atggcaaaca gatataatta aatttttatg actaaaaatg aacaatattt
      181 tagtattaat aaagatgcat ggaataagaa aacaccggtg catgtagaat cttcttttta
      241 taaccaagaa gcatttttaa atggaaaaaa ttccttaaac gatattgaat tagatttatt
      301 aggaaatata caagataaaa atattttgca tttgcaatgt cattttgggc aagatacaat
      361 ttcattaagt aggttaggtg caattgcaac aggtgtagat atagcagaaa atgcaataaa
      421 catagcaaaa caaactgcta accaactaaa cttgcaaaca caatttatat gctgtaatat
      481 ttatgattta tcaaattatt taaaccaaaa atttgatata gtttttacca gttatggaac
      541 cattggttgg ttgccagatt tagataaatg ggcaaaaatt attaaccatt ttttacaacc
      601 caatgggcaa tttattatgg tagattttca ccctgttgtt tggatgtttg ataacgattt
      661 tcaacaagta acttataact atttcaacac aggtgcaata cttgaaaatg aagtgggtac
      721 ttatgcaaac aaaaaaactg atattcaatt tcaaacagct tcctggaatc atagcattgg
      781 cgaaatactt aatagtttaa ttagtaacca atttagtatt aatagttttg atgagtatga
      841 ttattcacct tacaattgtt ttaataatac aattgaagtg gaaactaata aatttagaat
      901 aaaacatatg ggcaataaac tacccatgct ttatgctata aaagccacaa aagataaaac
      961 gtaaacataa actaattttc aataatagtg tttaagggta tccaattcta aactcttaca
     1021 tttgcatcaa attttttgct atggatatta aaaataacat actagaaact attggtaata
     1081 cacccttaat taagttaaat aaagttacaa aagattttcc ctgtactgtt ttagctaaag
     1141 tagattattt taacccaggc aatagcatta aagatagaat ggctttaaaa atggtagaag
     1201 tagccgaaaa aggaggtaaa cttaaaccag gtggaaccat tattgaatgt actagtggca
     1261 acacaggtat gggacttgcc ttagcagctt gtgtaaaagg atacaaatgt atttttgtta
     1321 caacagataa gcagagtaaa gaaaaagcag atattttaaa agcagtgggt gcagaagtta
     1381 ttgtttgccc aaccaatgta atgccagaag atcctaaaag ttattatagt gtggccaaac
     1441 gcttagctac tgaaataccc aattcatacc actttaatca gtacgataat ttagcaaacc
     1501 gtttagcaca ttacgaaagt acaggaccag aaatttggaa acaaactgat ggaaaaataa
     1561 cacatcttgt ttgcactgca ggaacaggag gtacaattac aggtactgca aaatatttaa
     1621 aagaaatgaa ccccaatatt caaatttggg caattgat
//
LOCUS       DAOQHP010000431         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_62287_length_1656_cov_4.047470, whole genome shotgun sequence.
ACCESSION   DAOQHP010000431 DAOQHP010000000
VERSION     DAOQHP010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62287_length_1656_cov_4.047470"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLY81_10605"
     CDS             <1..381
                     /locus_tag="PLY81_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30721.1"
                     /translation="MEGVTSTFKLKQKKQFFFYSALIWLMYLAGTWLGFYATQGTTGL
                     GVDAALSCLAFASIGMIITPGGIGAYAALLAIVLTLQNPPIPNAIGYANGTLQWFAQC
                     IIVLIVGFICIIFLPLYNKSKKVN"
     gene            467..1648
                     /locus_tag="PLY81_10610"
     CDS             467..1648
                     /locus_tag="PLY81_10610"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNF30722.1"
                     /translation="MNTRTVYIVSAVRTPLGSFGGILKDLSATQLGAVAISAAVEKAG
                     LEPEQIQEVIMGCVIQANLGQAPARQAAKFAGLPDNVVATTVNKVCASGMKAIAQAAQ
                     SILLGDADIVVAGGMESMSNVPFYSPNLRWGNKYGNVSMIDGLAKDGLTDVYFNGAMG
                     NFADATAKECNITREEQDAFAIESYTRSQNAWKEGRFANEVVPVVIESKKGNIVIDKD
                     EEPFNVKFDKIAGLRPAFNKDGTVTAANASTMNDGAAALVLMSKEKADELGIKPLAII
                     NSYADAEQAPEWFTTTPSLAVPKAVTKAGLTLNEIDFFELNEAFSVVGIVNCNKMNIP
                     FNKVNVHGGAVSIGHPLGCSGARIIVTLINVLKQNNGKIGAAGICNGGGGASAMVISL
                     P"
BASE COUNT          553 a          226 c          347 g          530 t
ORIGIN      
        1 atggaagggg taacttctac ctttaaatta aaacaaaaaa agcaattctt tttttactca
       61 gcactaattt ggttaatgta tttagcaggc acttggcttg gcttttatgc aacccaaggt
      121 actactggct taggtgttga tgcagcttta tcttgcttgg catttgctag cataggtatg
      181 attattacac ctggtggtat tggtgcttat gcagctttat tggcaatagt tttaactcta
      241 caaaatccac caatacccaa tgcaataggt tatgccaatg gtactttaca atggtttgca
      301 caatgtatta ttgtattaat tgtaggcttt atttgtataa tatttttgcc tttatacaat
      361 aaatcaaaaa aggttaatta aaatattttc tttttttata acctttaaaa aatcatacta
      421 acatttgtta ggttttgtgt aaatttgcca cttaaattta ttataaatga atactagaac
      481 agtttatatt gttagtgcag tacgtacacc attaggtagt tttggcggta ttttaaaaga
      541 tttatctgct acacaattag gagcagttgc aattagtgct gctgtagaga aagcaggctt
      601 agagccagag caaattcagg aagtaataat gggttgtgta attcaggcaa atttaggcca
      661 ggcaccagca cgccaggcag caaagtttgc aggattacca gataatgtgg ttgctacaac
      721 tgtaaataaa gtttgtgcaa gcggaatgaa ggcaattgca caagctgccc aaagtatttt
      781 attgggagat gcagatatag ttgttgcagg tggtatggaa agtatgagta atgtgccttt
      841 ttattctcct aatttacgtt ggggaaataa atatggaaat gtttcaatga ttgatgggtt
      901 agctaaagac ggcttaacag atgtatattt taatggtgca atgggcaatt ttgctgatgc
      961 aacagctaaa gaatgtaata taacaagaga agaacaagat gcttttgcaa ttgaaagtta
     1021 tacccgtagc caaaatgcat ggaaggaagg aagatttgct aatgaagtag tacctgttgt
     1081 aatagaaagt aaaaaaggaa atattgttat tgataaagat gaagaacctt ttaatgttaa
     1141 gtttgataaa attgcaggcc ttagaccagc attcaataaa gatggtacag taactgctgc
     1201 aaatgctagc accatgaatg atggagctgc agcattagtt ttaatgagta aagaaaaagc
     1261 agatgaatta ggcattaaac ctttagcaat tattaacagt tatgccgatg ctgaacaagc
     1321 acctgagtgg tttaccacta caccaagcct ggctgtacca aaagctgtaa ctaaagcagg
     1381 gttaacctta aacgaaatag atttttttga attgaacgaa gcttttagtg ttgttggtat
     1441 tgtaaactgc aataaaatga atataccttt caacaaagta aatgtacatg gtggtgctgt
     1501 aagtattggg caccctttag gttgtagtgg agccagaatt attgtaacat taataaatgt
     1561 actgaaacaa aataatggta aaataggagc tgcaggtatt tgtaatggtg gaggaggggc
     1621 tagtgcaatg gttatttctt taccataatt atttta
//
LOCUS       DAOQHP010000432         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_63033_length_1645_cov_3.774214, whole genome shotgun sequence.
ACCESSION   DAOQHP010000432 DAOQHP010000000
VERSION     DAOQHP010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63033_length_1645_cov_3.774214"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLY81_10615"
     CDS             <1..777
                     /locus_tag="PLY81_10615"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNF30723.1"
                     /translation="HEGSGKLLTQYGELSFSYGDYLVLPRGTIYQITFNDSNNRLLIV
                     ESFSPIRFPKRYMSKHGQLLEHSPFCERDIRAPQNLITKDEQGDFIIKTKKKGILYGL
                     HYANHPFDVVGWDGCCYPYAFSIHDFEPITGRVHQPPPVHQTFEGNNFVICSFVPRLY
                     DYHPLAIPAPYHHSNIDSDEVLYYVDGDFMSRKNVTRGMITLHPAGIPHGPHPGAIEK
                     SIGKLETKELAVMLDTFHPLMLTKQALEIENENYTMSWAE"
     gene            966..>1645
                     /locus_tag="PLY81_10620"
     CDS             966..>1645
                     /locus_tag="PLY81_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNF30724.1"
                     /translation="MNFKNFQVPYHISDQYAKKAAYFSMEFAIHQPLKIYSGGLGFLA
                     GSHLRSAYELKQNMIGIGILWKYGYYDQTRNQDQTLQPVWLEKNYHFLEDTGIKFQIK
                     IHDAPVWVKIWYLNPETFGSAPLFLMSTDLPENDYVSQTITHRLYDANVSTKVAQFIL
                     LGVGGAKLLEEIGFNTDVYHLNEAHGISAAFYLLANKYKTVNALKEHLVFTTHTPEEA
                     GNEKHDIY"
BASE COUNT          578 a          242 c          276 g          549 t
ORIGIN      
        1 catgaaggaa gcggtaaatt attaacccaa tatggtgaac ttagtttttc ttatggcgac
       61 tatttggttt taccaagagg aactatttat caaataactt tcaacgatag taataatagg
      121 ttgttgattg tggaaagctt tagcccaatt cgttttccca aaagatatat gagcaaacat
      181 gggcagttgt tagagcattc gccattttgt gaaagagata ttagggcacc tcaaaattta
      241 ataaccaaag atgaacaagg cgattttatt ataaaaacta aaaagaaagg aatattgtat
      301 ggtttgcatt atgctaacca tccttttgat gttgtaggtt gggatggttg ttgttaccct
      361 tatgcattta gtattcacga ttttgaacct attactggta gagtacatca accaccacct
      421 gttcaccaaa ctttcgaagg taataatttt gtgatttgta gttttgtacc acgcttgtac
      481 gattatcatc cattggcaat tcctgcacct tatcatcaca gtaatattga tagtgatgaa
      541 gttttatatt atgtagatgg agattttatg agtagaaaaa atgtaactag aggcatgata
      601 actttgcatc ctgcaggcat accacatgga ccacatcctg gtgcaattga aaaaagcatt
      661 ggtaaattag aaacaaaaga attagcggta atgttagata catttcatcc attaatgctt
      721 actaaacaag cattagaaat agaaaatgaa aattatacta tgagctgggc cgaatagttt
      781 tttaaaaaca ttgtaaacaa acccttgata acatcagggg tttatttttt ttcaacaatg
      841 ctgtataact taccttttac aagtttcggt ttgtatattt ttattgcctt aaaataatat
      901 tcaaattttc taccattatt attaattttt gaaagtttga aattttgtaa ctaaaaaaac
      961 attagatgaa ttttaaaaat tttcaagtac cttatcatat aagcgatcag tatgcaaaaa
     1021 aagcagccta tttttctatg gagtttgcaa tacaccaacc tttaaaaatt tacagtggtg
     1081 gtttaggctt tttagctggc tcgcatttac gtagtgctta tgaattaaag caaaacatga
     1141 ttggtatagg tattttatgg aaatatggtt attacgacca aacaagaaat caagaccaaa
     1201 ctttacaacc cgtatggtta gaaaaaaatt accatttttt agaagataca ggaattaaat
     1261 ttcaaataaa aatacatgat gctccagtat gggttaaaat atggtactta aacccagaaa
     1321 cttttggtag tgcaccatta tttttaatga gtaccgattt gccagaaaat gattatgttt
     1381 cgcaaacaat tacacaccgt ttatacgatg ccaatgtatc taccaaagta gcacagttta
     1441 ttttattagg tgtaggtggt gcaaagcttt tagaagaaat tggttttaat acagatgtat
     1501 atcacttaaa cgaagctcat ggtatttctg ctgcatttta tttactagca aataaatata
     1561 aaacagtaaa cgctttaaaa gagcatttag tttttaccac acacacacca gaagaggcag
     1621 gcaatgaaaa acatgatatt tattt
//
LOCUS       DAOQHP010000433         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_64035_length_1631_cov_2.906726, whole genome shotgun sequence.
ACCESSION   DAOQHP010000433 DAOQHP010000000
VERSION     DAOQHP010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64035_length_1631_cov_2.906726"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..>1631)
                     /locus_tag="PLY81_10625"
     CDS             complement(265..>1631)
                     /locus_tag="PLY81_10625"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNF30725.1"
                     /translation="LSEAVQEANKLGIILKGDIPIGINKNSVDCWQYPQLFNCNMQAG
                     APPDDFTEKGQNWGFPTYNWQVMQEDNFLWWKLRFQQMQCYYDAFRIDHILGFFRIWS
                     IPSNAVEGVMGYFVPAIPLQLNDFNKSGIFFNYVRYTQPFITDEILESFFDEYKEEVI
                     NQFLIKDDFSDNYTLTEKFSTQILVENFFSALPNNTKNNKIKQGLFNLISNVILLDID
                     GNENEFHFRFNMFKTTSFKQLSPIVQQQLLKLYNNYFFEQQNELWKKEGIYKLTTIKC
                     ATNMLVCGEDLGLVPAIVPEVMKSLGILSLEIQRMPKQNQQHFLQLNQLPYLSVAMPS
                     THDMSTIRSWWQEDFTNTKLFYNTVLNKTNHLPQECTVDISKEIIKQHLYSSAMWSIF
                     LLQDLFGIDENLRIENHQQERINNPANKNQIWNYRMHISLEKLLRQFTFNAELKKQIQ
                     LSGR"
BASE COUNT          538 a          273 c          223 g          597 t
ORIGIN      
        1 gccatctgga tagtaaaaat cccaattgtt tgggtcgtac acttcgaaat aggttttatc
       61 gtctacttcc acatagccct tcaccactat gaaatgaccg ctgtcaaaca cgtagaaacg
      121 gttgacacga gtggcttctg tagaataagg aacctttcca atctctgtac acagaagcga
      181 gatttcgcca tttttaagag aggtcttgag aaggttgatg ttgtcttgat aagtactttt
      241 gttaaaagaa agatatttat aaacttatct tccacttaat tgaatttgct tttttaactc
      301 tgcattaaaa gtaaactgtc gtaataactt ttctaaactg atgtgcattc tataattcca
      361 tatttgattt ttattggctg ggttatttat tctttcttgt tggtggtttt caattctcaa
      421 attttcatct attccaaata aatcttgtag taaaaaaatg ctccacattg cagaagaata
      481 taaatgctgt ttaataattt ctttactaat atcaaccgta cactcttggg gcaaatgatt
      541 agttttattt aatacagtat tataaaaaag tttagtatta gtaaagtcct cctgccacca
      601 acttcttatt gtactcatgt cgtgtgtaga gggcattgca acacttaaat atggtagttg
      661 attaagttgt aaaaaatgtt gctgattttg tttaggcatt ctttgaattt ctaaacttaa
      721 aatgcctaag cttttcatta cctctggaac aattgctggc actaaaccta aatcttcgcc
      781 acaaacaagc atattggttg cacatttaat tgttgttagt ttataaatcc cttctttctt
      841 ccataactca ttttgttgct caaaaaaata gttgttatac aacttcaata actgctgttg
      901 tacaattggt gaaagttgtt taaaagaagt tgttttaaac atgttaaacc taaagtgaaa
      961 ctcattttca tttccatcta tatccaacaa aataacatta cttattaaat taaataaacc
     1021 ttgtttaatt ttattgtttt tagtattatt gggcaaagca gaaaagaaat tttctactaa
     1081 tatttgagtt gaaaattttt cagtgagagt ataattatcc gaaaaatcat cttttattaa
     1141 aaactgatta attacttcct ctttatattc atcaaaaaaa gactcaagaa tttcatctgt
     1201 tataaaaggt tgggtatatc ttacataatt aaaaaaaata ccacttttat taaagtcatt
     1261 taattgcaaa ggaattgcag gtacaaaata acccattaca ccttctacag cattagatgg
     1321 aatactccaa attctaaaaa aacctaaaat atgatctatc ctaaaagcat cataataaca
     1381 ttgcatttgt tgaaacctta gtttccacca caaaaaatta tcttcttgca ttacttgcca
     1441 attataagta ggaaagcccc agttttgccc tttctctgta aaatcatctg gaggtgctcc
     1501 tgcttgcata ttgcagttaa ataattgtgg gtattgccaa caatcaacac tatttttatt
     1561 tatcccaatt ggaatatctc cttttaaaat aatgcctaac ttatttgcct cttgtacagc
     1621 ttctgataat t
//
LOCUS       DAOQHP010000434         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_64813_length_1620_cov_3.994249, whole genome shotgun sequence.
ACCESSION   DAOQHP010000434 DAOQHP010000000
VERSION     DAOQHP010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64813_length_1620_cov_3.994249"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..540
                     /locus_tag="PLY81_10630"
     CDS             31..540
                     /locus_tag="PLY81_10630"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNF30726.1"
                     /translation="MKIFLIGMMGSGKTYLSKLIADKIKFTFYDLDQIIEKRAGVEIN
                     NIFEEQGELYFRKIETEELGKFENKNNLVLATGGGTPCFNNNMNWMNKHGITIWLNEP
                     LDIICERLMKEKATRPLLKNKKNIEPYLKELLASRIDFYKKATYHFTYSDYNFNNLIS
                     TINQLKENE"
     gene            533..919
                     /locus_tag="PLY81_10635"
     CDS             533..919
                     /locus_tag="PLY81_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNF30727.1"
                     /translation="MNNTILKLAAFLGALSVALGAFAAHGLKKLISEKAVETFQTGVT
                     YQFYHVFALLAAGILFRYYKNKFIQNASIFFLLGILFFSGSLYLLTLKEYLQLESLQW
                     VVFITPLGGVCFIVGWMFLLLGIKEK"
     gene            complement(975..>1620)
                     /locus_tag="PLY81_10640"
     CDS             complement(975..>1620)
                     /locus_tag="PLY81_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF30728.1"
                     /translation="FRGPNASAGQLGAQHSSAFESLLANIPGIKVVSPSNGYDAKGLL
                     KQAIRYEEDPVCFLESEVMYGDKSEIPDEEYYIPLGKADVKKIGKDVTIVSFNKMMKV
                     ALNAATELEKEGISAEVIDLRTIRPLDWQTILESVKKTNRLVIVEEQWPFASVSSEIT
                     YRIQKEGFDYLDAPIRRITSADAPMHYAPNLVEAYLPSVERTVKLVKEVMYLQA"
BASE COUNT          554 a          229 c          247 g          590 t
ORIGIN      
        1 aaaagaaatt actagttgta taattaacaa atgaaaatat ttttaattgg catgatgggc
       61 agtggtaaaa cttatttaag taagctaatt gcggataaaa taaaatttac tttttatgat
      121 ttagaccaaa taattgaaaa aagggctggt gttgaaataa acaacatttt tgaagagcaa
      181 ggtgaacttt attttagaaa aattgaaaca gaagagttag gcaaatttga aaataaaaat
      241 aatttggttt tagcaactgg tggtggtaca ccatgtttta ataataatat gaattggatg
      301 aataagcatg gaattacaat ttggttaaat gagcctttag atattatttg tgagagatta
      361 atgaaagaga aagcaaccag acctttgtta aagaataaaa agaatataga accttacttg
      421 aaagaactat tagcctcacg aattgatttt tataaaaaag ctacttacca ttttacctac
      481 tctgattata atttcaataa tttaatttca acaattaatc aattaaaaga aaatgaataa
      541 tacaatatta aaacttgcag catttttagg tgcattaagc gttgcattag gggcttttgc
      601 agcacatggt ttaaaaaaat taattagcga gaaagcagta gaaacttttc aaactggtgt
      661 tacttaccaa ttttatcatg tatttgcttt gcttgcagct ggtatattat ttaggtatta
      721 caaaaataaa tttatacaaa atgcttctat atttttcctt ttaggaattt tgttttttag
      781 tggttcgctt tatcttttaa cactaaaaga gtatttgcaa ttagaaagtt tacaatgggt
      841 tgtttttatt acacctttag gaggtgtttg ttttattgtt ggttggatgt ttttattatt
      901 agggataaaa gaaaaataaa aaacctatca acttgtaaag tgataggttt taaataagta
      961 gttcaactaa aatattatgc ttgtaaatac attacctctt ttaccaactt aacagttctt
     1021 tcaacactag gtaaatacgc ttctaccaaa ttaggtgcat agtgcattgg tgcatctgca
     1081 cttgtaattc ttcttatagg agcatctaag taatcaaatc cttctttttg aattctataa
     1141 gttatttctg agcttacact tgcaaatggc cattgttctt ctactataac taaacggttg
     1201 gtttttttaa cactttctaa aatggtttgc cagtctaatg gacgaatagt tcttaagtca
     1261 attacttcgg cactaatacc ttctttttct aattctgtag cggcatttaa agctactttc
     1321 atcattttat taaatgatac aatggttaca tctttcccaa tttttttaac atctgcttta
     1381 ccaagtggaa tataatattc ttcatctggg atttcagatt tatcaccata cattacttcg
     1441 ctttctaaaa aacaaactgg atcttcctca tatctgatag cttgttttaa taatcctttt
     1501 gcatcatacc cattgctagg cgaaaccact tttatacctg gaatatttgc taataaactt
     1561 tcaaaggcag agctatgttg tgcacctaac tgccctgcac ttgcattagg gccacgaaaa
//
LOCUS       DAOQHP010000435         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_64902_length_1619_cov_3.829284, whole genome shotgun sequence.
ACCESSION   DAOQHP010000435 DAOQHP010000000
VERSION     DAOQHP010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64902_length_1619_cov_3.829284"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLY81_10645"
     CDS             <1..286
                     /locus_tag="PLY81_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF30729.1"
                     /translation="NQKIIVGVNKFTIDKEEAIPAFKIDDSIRQVQSNKLQNLKDKRN
                     NQKATNSLKAIEAAAINNTNLMPVVIEAVENYCTLGEIADCLRKVFGEYK"
     gene            339..>1619
                     /locus_tag="PLY81_10650"
     CDS             339..>1619
                     /locus_tag="PLY81_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30730.1"
                     /translation="MKFIAIKKTILLFTCIVLLQLISTAQRRRITADGTTGTTVTHDA
                     QGRPLPPKPKGTTDSLQKRDRYADSITIFYRYYDSSSIRFLDSTINDFAKRFNQPYFY
                     QNNGSYGTASRSLLFMPTLKAGFDVGINQYNIYDYTVEDTRFFQTTRPYTELAYVLAT
                     KAEQTINVLHTQNKKDNFNFALEYKFINSPGSYKTQNNNHNNIRVNANFKTNKRKYAA
                     QLIVISNKHIVSENGGLRDASFLDSLSFNDPFQLETRLGASGAYSQNPFNTNISTGNK
                     YKNNIFLFKHYYDVGKKDSTLNIQDSIYHKIFYTRFRLQHTLQSKSYSYQFVDANVEQ
                     TNYIKYFNFTVPNNNSISVENKWNVVENQFSIITFPDKNNQAQFLKLSAALQNIHRKD
                     SATSSNNHNFFISGEYRNKTRNNIWDVDAAGSLYI"
BASE COUNT          622 a          238 c          233 g          526 t
ORIGIN      
        1 taaccaaaaa attatagttg gggtaaataa atttacaata gacaaagaag aagctattcc
       61 tgcttttaaa attgatgata gcattagaca ggttcaatca aataaacttc aaaacctaaa
      121 agataaaaga aataatcaaa aagccactaa ttctttaaaa gcaattgaag cagcagctat
      181 caataacact aatttaatgc ctgtagtaat tgaagctgtt gaaaattatt gcaccttagg
      241 cgaaattgca gattgtttaa gaaaagtttt tggagaatac aaatagtatt cgctaatttt
      301 tacacttcat ttgcagccat tacagttaat ttgcagctat gaaatttatt gcaataaaaa
      361 aaacaatact actttttact tgtattgtat tgttgcagct tatttctact gcacaaagaa
      421 gaaggattac cgcagatgga acaactggca caactgtaac acatgatgca caaggcaggc
      481 ctttacctcc aaaacctaaa ggcacaacag actctctaca aaaaagagat agatatgcag
      541 actctatcac tatattttac aggtattacg actcttcttc tatcagattt ttagattcta
      601 ccattaacga ttttgctaaa agatttaatc agccatattt ttatcaaaac aatggaagtt
      661 atggtacagc ttcaaggtcg ttattattta tgcctacttt aaaagcaggg tttgatgttg
      721 gaataaacca gtacaatatt tatgattata ctgtagaaga tacaagattt tttcaaacca
      781 ccaggccata tacagagctt gcttatgtgt tagccaccaa agcagaacaa actattaatg
      841 tgttgcatac tcaaaacaaa aaagataatt ttaattttgc tttagagtat aagtttatta
      901 atagccctgg tagttataaa acacaaaata ataatcataa taatattagg gttaatgcca
      961 attttaaaac taataagcgt aagtatgctg cacaattaat tgttatttca aacaaacata
     1021 tagtttctga aaatggtgga ttaagagatg ccagtttttt agatagcctt tctttcaacg
     1081 atccttttca acttgaaaca aggttaggtg catctggtgc ttattctcaa aaccctttta
     1141 atactaatat ttcaacaggt aataaatata aaaacaatat ttttttattt aaacattact
     1201 atgatgtagg taaaaaagat tctacattaa atatacaaga ctccatttat cataaaattt
     1261 tttatacaag attcaggttg caacatacat tacaatctaa aagctatagt tatcagtttg
     1321 tagatgccaa tgttgagcaa actaattaca ttaaatattt caattttact gtacctaata
     1381 ataatagtat tagtgtagaa aataaatgga atgttgtaga aaatcaattc agcattatta
     1441 cttttccaga taaaaataat caggctcagt tcttaaaact gagtgcagcc ctgcaaaata
     1501 ttcatagaaa agattctgca acaagcagta ataatcataa tttttttata tcgggtgagt
     1561 atagaaataa aacaagaaat aatatttggg atgtagatgc tgctggtagt ttatatatt
//
LOCUS       DAOQHP010000436         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_64981_length_1618_cov_4.753039, whole genome shotgun sequence.
ACCESSION   DAOQHP010000436 DAOQHP010000000
VERSION     DAOQHP010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64981_length_1618_cov_4.753039"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1618)
                     /locus_tag="PLY81_10655"
     CDS             complement(<1..>1618)
                     /locus_tag="PLY81_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNF30731.1"
                     /translation="SKTYYKFKVDFGIHPVTLQPIKRKLVRITKATLDSVGLGNTNVE
                     QFKLFRNGVEVPIFTSTTSGVLDSLGYIEFWGEINDGKLDKELYRDADYQLSDIWSLQ
                     EDNGTYFLTTSNAINKRLVNTANNVIGNLLSPTLYFMNTVMYTQRQRRFEGYAAQSSL
                     PLHSSSYDKGEGYATRPIRPIGSSCGQVSFTINFTNLKPYLSGPPMTLKVNTMGSENN
                     TRTILVKINGNSVSNYQMDYFNYAKVEESGIPVSYISSGNAAVLHINQSPVDCDEFRL
                     IKDELTYPRTLDGDNNVSLELKLPATATGHYLKFFNFNSGANSPILYDITNGKRYIAD
                     KTINDTLQFVTNPSLTAYNLVLVNNDSANIFSVSTLQQRNFVNYQNIANQGDYIIISN
                     PLIYGTGNSNYVEQYKQYRSSTKGGSYNAIVVDINEITDQFAFGINKHPLSVRNFLRY
                     ARNIFTTTPKYVFIIGKGLNYIDFRSNEDEPDMPYQNLVPTWGHPASDNLLSAADNLN
                     PTPITPIGRLSAVSANEVGDYLKKIKQYDSL"
BASE COUNT          518 a          256 c          252 g          592 t
ORIGIN      
        1 gtaatgaatc gtattgttta atttttttaa gataatctcc aacctcattt gcagatactg
       61 cacttaatct gccaattggt gttatgggtg taggatttaa attatctgct gcactaagta
      121 agttatctga tgcaggatgc ccccatgtag gtacaaggtt ttgataaggc atatctggtt
      181 catcttcatt acttctaaaa tcaatatagt ttaaaccttt accaattata aaaacatatt
      241 ttggtgtagt agtgaaaata tttcttgcat aacgtaaaaa atttcttaca gaaagtggat
      301 gtttatttat accaaatgca aactgatcgg ttatttcatt tatatctact acaattgcat
      361 tatagctgcc accttttgta ctacttctgt attgtttata ttgttcaaca taattactat
      421 ttccagtgcc atatattaat ggatttgaaa taatgatata atctccttgg ttggctatat
      481 tttgatagtt tacaaagttt ctttgctgaa gggttgatac agaaaaaata tttgcactat
      541 cgttgttaac taataccaaa ttataagcag ttaaagaagg attagtaaca aactgtaatg
      601 tatcattaat agttttatct gctatatatc ttttgccatt tgtaatatcg tacaaaatgg
      661 gtgaattggc tccagaatta aaattgaaaa attttaaata atggccagtt gctgttgcag
      721 gtaattttag ttctaagctt acattattgt ccccatctaa agttcttgga taagttaatt
      781 catctttaat taatctaaat tcatcacaat ctacagggct ttggttgata tgtaaaacag
      841 cagcattgcc agatgagata taacttacag gaataccaga ctcttcaact ttagcatagt
      901 taaaataatc catttgataa ttacttacgc tattgccatt tattttaact aaaatagttc
      961 ttgtattatt ttcactaccc atagtattca cctttaaggt cattggcggg ccacttaaat
     1021 aaggttttaa attggtaaag ttgatagtaa aagatacttg accacaacta ctaccaattg
     1081 ggcgaatagg acgagtggca tatccttctc ctttatcgta agaggaggag tgtaatggta
     1141 aggatgactg tgcagcatat ccttcaaacc ttctttgcct ttgtgtgtac attactgtat
     1201 tcataaaata caaagtagga gaaagtaggt tgcctataac attattggca gtgtttacta
     1261 gccttttatt tattgcgttg cttgttgtta aaaagtaagt accattgtct tcttgtaaac
     1321 tccaaatatc agataattgg taatcggcat cacggtataa ttctttatca agttttccgt
     1381 cattaatttc tccccaaaac tctatatagc ctaaactatc taaaacacca cttgtagtag
     1441 aggtgaaaat tggtacttct acaccattcc taaataattt aaattgttca acattggtat
     1501 tacctaaacc aacactatct aatgttgctt tagttattct taccagtttt cttttaatgg
     1561 gttgaagggt tacaggatgg ataccaaaat ctaccttaaa cttataataa gttttaga
//
LOCUS       DAOQHP010000437         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_65308_length_1614_cov_3.454137, whole genome shotgun sequence.
ACCESSION   DAOQHP010000437 DAOQHP010000000
VERSION     DAOQHP010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65308_length_1614_cov_3.454137"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLY81_10660"
     CDS             <1..1032
                     /locus_tag="PLY81_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30732.1"
                     /translation="NILQNIITVYDSVISRYTLSAASSHYLAIRLYYIDSVLSIPANQ
                     KNALVESYFMNCMQHKNRAYSDNFNTAFNAVFTQPQDTVYYAAAYNNEITRNSIEAAQ
                     VALSVYIKTYSLSAMAATQIIPYLVQRERAIALSNKLYANYSEAKDSLINNILLIHQP
                     IIDSVVSLYAHLYNNSQIDIAIKFATEIDLDENQLNTLHQAVATLKEMQTTFRETDPF
                     GEFDSKAYESEVLNSVLTPEQYTYVLEAKYYSKATAMANKDWTELVRLHIATELSLQE
                     AITKTELTNYHVAIFIAYYRNANNPEEQYISIQRINEVMPETMRLLFDRWAESGTPYG
                     NLPDVFFQW"
     gene            1187..>1614
                     /locus_tag="PLY81_10665"
     CDS             1187..>1614
                     /locus_tag="PLY81_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30733.1"
                     /translation="MKKILFILLLFSCYTAAYAQSDSTAITDSAELYLTGTFKKYNPQ
                     KAFQLFTLRANAGEPKAMNALGMHYAKGLGVDSNFALAKYWFLQAANNGYHKAWVNAG
                     MLYKTHSTDSTGYAAAVQYFISAVQLQEPSAFFALGYMYY"
BASE COUNT          600 a          266 c          264 g          484 t
ORIGIN      
        1 aatatcctgc aaaatattat tactgtttat gatagtgtaa taagcagata tacactttct
       61 gcagcaagta gtcattattt ggcaatcaga ttatattata tagatagtgt actaagcatt
      121 cctgctaatc aaaaaaatgc tttagtagaa tcttatttta tgaattgcat gcagcataaa
      181 aacagagcct actccgataa ttttaataca gcatttaatg cggtatttac acaaccgcaa
      241 gacaccgttt attatgctgc tgcttataac aacgaaatta caagaaacag tatagaagct
      301 gcacaggttg cattatcggt ttacattaaa acttacagtt tatctgcaat ggcggcaaca
      361 caaataattc cttacttagt acaaagagaa agagcaatag ctttaagtaa caaactgtat
      421 gctaattatt cagaagccaa agacagtttg ataaataata ttttactcat ccatcaacct
      481 ataatagata gtgtagtaag cttatatgcc catttatata ataactctca aatagatatt
      541 gccataaaat ttgctactga aattgattta gatgaaaacc agcttaatac actacatcag
      601 gcagttgcaa ctttaaaaga aatgcaaaca acgtttagag aaaccgaccc ttttggagag
      661 tttgacagca aagcctacga aagtgaagta ttaaactctg tgcttacacc agaacaatat
      721 acttatgtat tagaagcaaa gtattatagc aaagctactg ctatggcaaa taaagactgg
      781 acagaattgg taagattaca tatagccacc gaattaagtt tacaggaagc tattactaaa
      841 acagaactaa ccaattatca tgtagccata tttatagctt attaccgaaa tgccaataac
      901 ccagaagagc aatacatcag tattcaaaga ataaatgaag tgatgcccga aacaatgaga
      961 ttattatttg accgttgggc agaatccggt acaccttatg gcaatttacc tgatgttttt
     1021 tttcaatggt agttttttaa aatttaaaat taagaattaa aaattaaaaa ttataaatta
     1081 agaactagta gaaagcgtaa tgatgaatgt tgaatgttta atgatggaaa ctggtgtatc
     1141 taattcctaa ttacccataa aacgaataaa cctattaact gataaaatga aaaaaatatt
     1201 gtttatctta ttgttgtttt cttgctacac agcagcctat gcacagtctg atagtacagc
     1261 cattacagac agtgcagagt tgtatcttac tggcacattt aaaaagtata atcctcaaaa
     1321 agcatttcag ttatttaccc tacgagctaa tgcaggcgag ccaaaagcca tgaatgcctt
     1381 aggaatgcac tacgcaaaag gcttgggcgt tgatagcaat tttgctttag caaaatactg
     1441 gtttttacag gcagccaata atggatacca taaagcttgg gttaatgcag gtatgctgta
     1501 taaaacacac agtacagata gtacaggtta tgcagcagca gtgcaatatt ttatttcagc
     1561 agtacaatta caggaacctt ctgcattttt tgcattaggc tatatgtatt acaa
//
LOCUS       DAOQHP010000438         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_68292_length_1576_cov_3.375411, whole genome shotgun sequence.
ACCESSION   DAOQHP010000438 DAOQHP010000000
VERSION     DAOQHP010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68292_length_1576_cov_3.375411"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLY81_10670"
     CDS             <1..584
                     /locus_tag="PLY81_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNF30734.1"
                     /translation="ITCSNWSRNMFQPDWKITFVPTSPAIQTFNAALYYTGLGFLEAT
                     NISEGRGTQNAFEILGAPWLNNEMLCEVFNSLQDEVALQPYNFTPTFSKYKNEKCYGV
                     SLKVKNISVYHPVHTALLLIKTIKETHSTYFAWSTYPTMVNQSGEKHLDKLLGILNSE
                     NLLELSFQPFLKELNKITDSKDWQHKINSFLLY"
     gene            679..>1576
                     /locus_tag="PLY81_10675"
     CDS             679..>1576
                     /locus_tag="PLY81_10675"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HNF30735.1"
                     /translation="MKKSILLIALIGCSFLLKAQQKSYKISIIAFYNLENFYDTINDP
                     NKNDEEFTPEGPRNYNGAIYWDKVGKLATVISKLGSDEKPESLSPDGAAILGVAEVEN
                     ESVLKDLTNHPLLKKRNYQIVHYDSKDARGVDVAMLYNPKYFIVEDSKPLFVQLPGGA
                     KDAYFTRDILWVKGKLDGETVHVYVNHWPSRLGGEERSAPARNAAAMVCKKHIDSIAA
                     ADGEQKVVIMGDLNDNPTNESITQVLKAKAKIKDVRPGGLFNPWINLYKKGYGTLAYQ
                     DAWGLFDQIMISYPWLSQEQNGF"
BASE COUNT          591 a          219 c          272 g          494 t
ORIGIN      
        1 gtattacttg ttcaaattgg agcagaaata tgtttcagcc agactggaaa ataacatttg
       61 tgcctacatc gcctgcaata caaactttta atgctgcttt gtattataca ggattaggct
      121 ttttagaagc taccaatatt agcgaaggaa gaggaacaca aaatgcattt gaaatacttg
      181 gtgccccatg gttaaataat gaaatgttat gtgaggtatt taatagctta caagatgaag
      241 tagctctaca accatataat ttcaccccaa catttagtaa atataaaaat gaaaaatgtt
      301 atggggttag cttaaaagtg aaaaatattt cagtttatca tcctgttcat acagcattat
      361 tgttaataaa aacaattaaa gaaactcatt ctacatattt tgcttggagt acttacccaa
      421 caatggtaaa ccaatctgga gaaaaacatt tggataaatt actaggaatt ttaaatagcg
      481 aaaatttatt agagttatct tttcaaccat ttttaaaaga gttaaataaa ataacagatt
      541 caaaggattg gcagcataaa ataaatagtt ttttattgta ttaaaaatgt ttactaaaat
      601 tattaatatc ttaaaacttt attcaaaatt ttaaaactgc ttattcttga ttatgtttgt
      661 accttataaa atttacacat gaaaaaaagc atcttactta tagcactaat tggatgtagc
      721 tttttattaa aagcacaaca aaaatcttat aaaatatcca tcattgcatt ttataattta
      781 gagaattttt atgacaccat caacgatcca aataaaaatg atgaagaatt tacacccgaa
      841 ggaccaagaa attacaatgg tgccatttat tgggataaag ttgggaaatt agcaactgta
      901 atttcaaaac ttggtagcga tgagaagcca gaaagtttga gccccgatgg tgcagctatt
      961 ttaggtgttg cagaggtaga aaacgaaagt gttttaaaag atttaaccaa tcatccatta
     1021 cttaaaaaaa gaaattatca aattgttcat tatgatagta aggatgcaag aggtgtagat
     1081 gttgcaatgc tctacaaccc taaatatttt attgttgaag atagcaaacc actttttgta
     1141 caattacctg gtggtgcaaa agatgcctat tttacaagag atattctatg ggtaaaaggt
     1201 aaacttgatg gcgaaacggt acatgtttat gttaatcact ggcctagcag attaggagga
     1261 gaagaacgta gtgcaccagc tagaaatgct gctgcaatgg tttgtaaaaa acatattgat
     1321 agtattgctg ctgcagatgg tgaacaaaaa gtagttatca tgggcgactt aaacgataac
     1381 cctaccaacg aaagtattac acaagtactt aaagcaaaag ctaaaataaa agatgttaga
     1441 cctggtggtt tgtttaaccc ttggataaat ttatataaaa aaggttatgg cacattggcc
     1501 tatcaagatg cttggggttt gtttgaccaa ataatgattt cttacccatg gttaagtcaa
     1561 gagcaaaatg gatttt
//
LOCUS       DAOQHP010000439         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_69308_length_1564_cov_3.374420, whole genome shotgun sequence.
ACCESSION   DAOQHP010000439 DAOQHP010000000
VERSION     DAOQHP010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69308_length_1564_cov_3.374420"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1451)
                     /locus_tag="PLY81_10680"
     CDS             complement(join(268..1196,1196..1451))
                     /locus_tag="PLY81_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611811.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNF30736.1"
                     /translation="MKRVNNHYSLEFKLHAVELSKQKSLQEVANELNISRETLRNWTR
                     GMYLGQSQTKDSVISKPRSKEEEENIKLKKELYEIKLERDIFKKGSWHLLQERQVRFD
                     FIKKHKNEFPVEKMCKVLKVSTSAYYKWFNKTPSKRELRKTMLTQEIKRVYRIFKGRY
                     GSPRITKELNMQGIIVSRVLVAKIMKQEHLKSIIKKKYKVTTDSSHKYPVVENVLNRM
                     FNVKEENKVWVSDITYVGTKQGWLYLTTVIDLFDRKVIGWALSDTMKANATVIPAFNM
                     AKCNRPIQNNKSLIFHSDRGIQYACEEFTNHIAKQKTIIRSMSRKGNCWDNAVAESFF
                     KTLKTELIYHHKYQTKQEAALSIFEYIEAFYNTKRRHKQLKNLTILEYQKSINNNLKN
                     AA"
BASE COUNT          442 a          283 c          197 g          642 t
ORIGIN      
        1 cccctcctaa atgtgctatc agaactggcg gttttccaaa ccgctttttg ctatccctct
       61 acaaaaaatt aaaaactgtg ttccaatttt agcattcttt aactcactaa acacaaaaag
      121 acaacagtgt gtttcaaact gacaaattca ttttcaaatt aaaacacggt ggaaaccata
      181 gcaacatctt ttagcagtgt ggggcaacct aaaacctaac acacgctttg gaattccaaa
      241 caaaaagtgg acaaaaattt gttaacatta agccgcattt tttaagttat tattaattga
      301 tttttgatac tctaagatag ttaaattttt taattgttta tgccttcgtt ttgtattgta
      361 aaaagcttca atatattcaa aaatagataa agctgcttct tgttttgttt ggtatttatg
      421 atgatagatt aattctgttt ttagtgtttt aaaaaaactt tctgcaacgg cattatccca
      481 acaatttcct tttctactca tacttctaat aatagttttt tgtttagcga tatggttggt
      541 aaattcttcg caagcatatt gtatccctct atccgaatga aaaattaatg atttgttatt
      601 ttgtattgga cggttgcatt ttgccatatt aaaagcaggt attacagttg catttgcttt
      661 catcgtgtcg ctcaatgccc agccaattac ttttctgtca aataaatcaa ttaccgttgt
      721 caagtacaac caaccttgct ttgttccaac ataagtgata tcagacaccc acaccttatt
      781 ttcttcttta acattaaaca ttctgtttaa tacattttct actaccggat atttatgtga
      841 agaatcagta gttactttgt atttcttttt tataatactt tttaaatgtt cttgtttcat
      901 tattttagca actaataccc ttgatacgat tatgccttgc atatttaact cttttgttat
      961 tcttggacta ccgtaccttc ccttaaaaat cctgtaaact ctttttattt cttgagttaa
     1021 cattgttttt cttaattctc gtttacttgg tgttttatta aaccacttgt aataggcgct
     1081 tgtacttact tttaatactt tacacatctt ctcaaccgga aattcatttt tatgtttttt
     1141 aataaaatcg aatctcactt gtcgctcttg gagaagatgc caagagcctt ttttaatata
     1201 tcacgctcta attttatttc atacagttcc ttttttaatt taatgttttc ttcctcttct
     1261 ttactcctag gtttacttat aactgaatct tttgtttgag attgacctaa atacatgcct
     1321 cttgtccagt ttcttaaagt ttctctgcta atgtttaatt cattagctac ttcttgtaaa
     1381 ctcttttgtt tacttaattc tacagcgtgt aatttaaatt ctaaactgta atgattgttt
     1441 actcttttca tttgttaaag atagcgcagt taatcttaac ttttgtgtcc acctaaaggt
     1501 agtaactcca atcttgtgtt gttccttttg agttactgtt tacaatatct tgttgcgcta
     1561 caat
//
LOCUS       DAOQHP010000440         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_69562_length_1561_cov_3.245020, whole genome shotgun sequence.
ACCESSION   DAOQHP010000440 DAOQHP010000000
VERSION     DAOQHP010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69562_length_1561_cov_3.245020"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLY81_10685"
     CDS             complement(<1..1125)
                     /locus_tag="PLY81_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF30737.1"
                     /translation="MKKLLLLFSCLSVFSANSQNGFTTYTTNLSLTGTVMRQTSFVVD
                     NSGNKWIGFKSPIVSGNNVGLVKYNNNTWTLYNTTSNPALPANAITALASDNNGNIWI
                     GSPSGLIKFDGTSFTTYNSGLGAAVINCLEAVGNQVYVGTNSGLFRFDGSNFTGYNVG
                     NGKLPSDTVTCIKAESNNLIWLGGKDRLVQFNINAAYTVTSYSNLAIPNLPGNINCVY
                     IDGQNAKWLGTTNVGILKYTGSTFNNANSMYVLFGSSIPNKVVDIAAGVHNGIVFKHT
                     TGTTTMGTISGLSELAPNNTVYQYYYPVSTYNLGDYIEREGTKILISQGGTTVTYTSP
                     KMYYSFDYLNYVMPLGAVNPNNFKTLDINNVRAGIANRGDM"
     gene            1382..>1561
                     /locus_tag="PLY81_10690"
     CDS             1382..>1561
                     /locus_tag="PLY81_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNF30738.1"
                     /translation="MNERIQRESAFVDVLTSEMDKVIVGQKHMVERLLIGLLSNGHIL
                     LEGVPGLAKTLAINTL"
BASE COUNT          549 a          267 c          222 g          523 t
ORIGIN      
        1 catatcacct ctattagcaa tgccagctct cacattattt atgtctaatg ttttaaaatt
       61 gttaggatta actgctccta atggcattac ataatttaaa taatcaaaac tataatacat
      121 ttttggagat gtatatgtaa ctgttgtccc tccttgtgat attaaaattt tagtaccttc
      181 tctctctata taatctccca aattataagt gctaactgga taataatact gataaactgt
      241 attattaggt gctaactcag atagtccact tatagtaccc atagtagttg tacccgttgt
      301 gtgtttaaaa acaataccat tatgaacacc agcagcaata tcaacaactt tatttggaat
      361 tgaagagcca aaaagaacgt acatactatt agcattatta aaagttgaac cagtatattt
      421 taaaatacca acatttgtgg ttcctaacca ttttgcattt tgcccatcta tatatacaca
      481 gttaatatta cctggtaaat ttggtatagc taaattcgaa tagctagtaa ctgtatatgc
      541 ggcattaata ttaaattgaa ctaatctatc tttaccacca agccaaatta aattattaga
      601 ttctgcttta atacaagtaa cagtatcact aggcaacttt ccgttgccta cgttgtaacc
      661 agtaaaatta cttccatcaa acctaaacaa tccggaattt gttcctacat aaacttgatt
      721 acctacagct tccaaacaat ttataactgc tgctcctaac ccagaattat aagttgtaaa
      781 agaggttcca tcaaatttaa tcaaaccgga tggtgaacct atccatatgt taccattatt
      841 atcggaagct aaagctgtaa tagcattagc cggcaatgcc gggttagaag tggtattata
      901 caaagtccac gtattgttat tatattttac taatccaaca ttattgccac ttacaattgg
      961 ggatttaaac cctatccatt tattaccaga attgtcaaca acaaaagatg tttggcgcat
     1021 tacagttcct gttaatgaca gatttgttgt gtaggttgta aacccgtttt gtgaattagc
     1081 cgaaaatact gacaaacagc taaaaagtag taatagtttt ttcataaaaa atagttttag
     1141 ttacttcaaa attaacttta ttacttaact aatttttaac aactgagtaa ctggttgttt
     1201 ttttctaata actggtagaa aatagcctat aaatgttaaa tccaatttgg ataataaacg
     1261 ttaaaacaca ttacattgtt aacatatttt aacaagccta aaataaccga aacccttacc
     1321 attactaact ttgtgacgct tgtattaaac aaaaacactt atggtagtag atattagaga
     1381 attgaacgaa cgcattcagc gtgaaagtgc atttgtggat gtgttaacat ctgaaatgga
     1441 caaagtaatc gtaggccaaa aacacatggt tgaacgttta ttaattggct tattaagtaa
     1501 tggtcacatt ttattagaag gtgttccggg actagcaaaa acattagcta ttaatacatt
     1561 a
//
LOCUS       DAOQHP010000441         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_70054_length_1555_cov_3.365333, whole genome shotgun sequence.
ACCESSION   DAOQHP010000441 DAOQHP010000000
VERSION     DAOQHP010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70054_length_1555_cov_3.365333"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLY81_10695"
     CDS             <1..720
                     /locus_tag="PLY81_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNF30739.1"
                     /translation="ILFAYWLISLLAGNSADPFSITGHFARNIDIAVLTKYHMLHIDK
                     IDGKVFHFDYEGLASTTAAIAQVIMGYLIGYYIQQKGKTYEMLSNILVAAILFMAAGY
                     CWDMVFPINKKIWSSSYTIYTTGLAIATIGVLIYLLEFKNAKGAWSKFFDVFGKNPLF
                     IFVLSGFLPRLLTLFRINDHTDENGNSVYLSAFGWFYEHICKNISTDLRVGSLVYAIS
                     MIVMYWFIVYLLDKKKIYIKV"
     gene            784..>1555
                     /gene="bshA"
                     /locus_tag="PLY81_10700"
     CDS             784..>1555
                     /gene="bshA"
                     /locus_tag="PLY81_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNF30740.1"
                     /translation="MRIGIVCYPTFGGSGVLATELGKALADKNHQVHFITYQQPVRLN
                     IFNANIFYHEVQVPTYPLFDYPPYEIALSSTMVDVIIKHDLDLLHVHYAIPHASAAYM
                     AKQIVLKKTGRNIPVITTLHGTDITLVGRDKTYEPVVTFSINESDAITAVSENLKEET
                     LKHFEINKPIEVITNFVDIERFGKKPIDAFKKVIAPNAEKIFLHASNFRKVKRVEDVI
                     IIFDEVRKHLPAKLLMVGDGPERPAAEELVRTLNLEPDV"
BASE COUNT          540 a          223 c          266 g          526 t
ORIGIN      
        1 atattatttg cttactggtt aataagttta ttagctggta attctgcaga ccctttttct
       61 attacagggc attttgccag aaatattgac attgctgtat taaccaaata ccacatgtta
      121 catattgata aaattgatgg caaagttttt cattttgatt atgaagggct ggcaagtaca
      181 actgctgcaa tagcacaggt tattatggga tatttaattg gatattatat tcaacaaaaa
      241 ggaaaaactt acgaaatgct tagcaatata ctggtagctg caattttatt tatggcagct
      301 ggctattgtt gggatatggt ttttcctatc aacaaaaaaa tatggagcag tagctatacc
      361 atttatacaa cagggcttgc cattgcaact attggagtat taatttattt gcttgaattt
      421 aaaaatgcta aaggtgcatg gagtaaattt tttgatgtat ttggaaaaaa tcctttgttc
      481 atatttgtgc tgagtggctt tttaccacgt ttgcttaccc tgtttagaat taatgatcat
      541 actgatgaaa atggcaactc ggtttatcta tctgcctttg gttggtttta tgaacatatt
      601 tgcaaaaata tttcaaccga tttaagagta ggttcattag tttatgcaat tagtatgatt
      661 gttatgtatt ggtttattgt ttatttatta gataagaaga aaatttatat aaaagtataa
      721 acccttttct tccttgaaag tataagctta ccttcatcat ctttaaagta taaaaaataa
      781 aaaatgcgta taggaattgt ttgttatcca acttttggcg gaagcggagt tttggcaact
      841 gaattaggca aagctttagc agataaaaac catcaggtac attttattac ttaccagcag
      901 ccggttaggt taaacatttt taatgctaat attttttacc acgaagtaca agttcctact
      961 tatcctttgt ttgattatcc accatacgaa attgccctat ctagtacaat ggtagatgta
     1021 attattaaac acgatttaga tttactgcac gttcattatg ccataccaca tgcaagtgct
     1081 gcatatatgg ccaagcaaat tgtattaaag aaaaccggaa gaaatattcc tgtaattact
     1141 acattacatg gaacagatat aaccttagtt ggcagagata aaacctacga acctgttgta
     1201 acattttcta ttaacgaaag tgatgctatt accgctgtaa gcgaaaactt aaaagaagaa
     1261 accttaaaac acttcgaaat aaataaacct attgaggtaa taaccaactt tgtagatatt
     1321 gaaagatttg gcaaaaagcc tattgatgct tttaaaaaag taatagcacc aaatgccgaa
     1381 aaaatatttt tacatgctag taattttaga aaagttaaaa gagtagaaga tgtaattata
     1441 atatttgatg aagtgagaaa acatttacct gccaaattac taatggttgg cgatggacca
     1501 gaaagacctg cagcagaaga attagttaga actttaaatt tagaacccga tgtaa
//
LOCUS       DAOQHP010000442         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73057_length_1520_cov_2.839590, whole genome shotgun sequence.
ACCESSION   DAOQHP010000442 DAOQHP010000000
VERSION     DAOQHP010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73057_length_1520_cov_2.839590"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLY81_10705"
     CDS             complement(<1..441)
                     /locus_tag="PLY81_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30741.1"
                     /translation="MAINFIQAQYTTISGVVYDISAKRPIEAVAVISTSGKGTITDSL
                     GRYQITVKNTDSIWFSLIGKSTMKYVVDTISNPFAFDVMIHVHVAQLPEVKVRNKNYR
                     LDSIQNRKDYAYVFNYKKPSISLLQTPSYNSTPGVSVGIDLIEFI"
     gene            583..>1520
                     /locus_tag="PLY81_10710"
     CDS             583..>1520
                     /locus_tag="PLY81_10710"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF30742.1"
                     /translation="MLFPRLTHTTDIAKLIDAEVLGKVDAFAKGINEIHKVNEGDICF
                     VDHPKYYSKCLNSAATFIIINTKDITIPAGKTIFFVKEPFEAYLKIVHHFRPFTSQQH
                     AINDNVVIGKDTVIMPNVYIGNHVKIGNNCIIYPNVSILDFTEIGNNVVIQSGTVIGS
                     NAFYYNTKKTREVWYKRMDSCGNVILEDFVEIGASCTIDRGVTASTIIGKGTKLDNMV
                     HIGHDTCIGKNCLIAAQVGIAGATIVEDGVTLWGQVGVNKTITIGKNAVVLGQSGVTN
                     SLEGDKVYFGLPAEDASLKRRELVWIKRIPELWKKV"
BASE COUNT          552 a          201 c          262 g          505 t
ORIGIN      
        1 aataaactct attaaatcaa taccaacact tacaccaggg gttgaattat aagaaggagt
       61 ttgtaataag ctaatacttg gttttttata attgaaaacg taagcataat cttttctgtt
      121 ttgaatagaa tctaacctgt aattcttgtt tctaacttta acttctggca attgagctac
      181 atgaacatgt atcataacat caaatgcaaa agggttacta atagtatcca caacatactt
      241 catggttgat ttaccaatta aagaaaacca aatagagtcg gtgtttttta ctgtaatttg
      301 ataccttcct aaagagtctg taatagtacc ttttcctgaa gtactaatta cagcaactgc
      361 ttcaattggg cgttttgcag aaatatcata cactacacca ctaatggtag tgtattgtgc
      421 ttgtataaaa ttaattgcca ataaacagac taataaagta aatagctttt taataaacaa
      481 aatatttttg taatattaat aaagcaacac tgattatagc actataattt attttattaa
      541 ctgttttttg tagattatat acctttgccc tcaaattttt atatgctttt tcctcgtcta
      601 actcatacaa cagatattgc aaaacttatt gatgctgaag ttttaggaaa agtggatgct
      661 tttgcaaaag gaattaatga aattcataag gtgaatgaag gagatatttg ttttgtggat
      721 catccaaaat attattcaaa atgtttaaat agtgctgcta catttataat aattaataca
      781 aaagatataa ctatacctgc gggcaaaact attttttttg taaaggaacc ttttgaggca
      841 tacttaaaaa ttgtacatca tttcaggcca tttaccagcc agcaacatgc aataaatgac
      901 aatgttgtta taggaaaaga tacagtaata atgcccaacg tttatattgg taaccatgta
      961 aaaattggta ataattgcat catttatcca aatgtttcta ttttagattt tacagaaatt
     1021 ggtaacaatg ttgttataca atctggcact gtaataggta gtaatgcatt ttattataat
     1081 accaaaaaaa caagggaggt ttggtataaa agaatggata gctgtggtaa tgtaattctt
     1141 gaagattttg tagaaatagg agcaagctgc actattgata gaggtgtaac tgcaagtact
     1201 attattggta aaggaactaa gttagataat atggtacata taggccatga tacatgcata
     1261 gggaaaaact gtttaatagc tgcacaagtg ggcattgcag gtgccactat tgtagaagat
     1321 ggagttacac tttgggggca agttggagta aacaaaacaa ttactattgg taaaaatgct
     1381 gttgtactag ggcaaagcgg tgttaccaat tctttagaag gagataaagt ttattttgga
     1441 ttaccagctg aagatgcatc tttaaaaaga agagaattag tttggataaa aagaatacct
     1501 gagctttgga aaaaagtaat
//
LOCUS       DAOQHP010000443         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73334_length_1517_cov_3.621751, whole genome shotgun sequence.
ACCESSION   DAOQHP010000443 DAOQHP010000000
VERSION     DAOQHP010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73334_length_1517_cov_3.621751"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLY81_10715"
     CDS             complement(<1..728)
                     /locus_tag="PLY81_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNF30743.1"
                     /translation="MNKELIINTTPIGVEIALLEDKKLVELHNEKSDANFGVGDLYLG
                     KVKKLIPGLNAAFVDVGFEKDAFLHYTDLSPFARSILKFTQQAMAANENSFEFSNFQI
                     EPEIIKSGKINEVLNGKPNVLVQILKEPIAAKGPRLSCEISLPGRFIVLTPFNEIVAV
                     SKKIHSGEERKRLQKIVESIRPKNFGVIVRTAAEGKSTAELHQDLLELTETWKTIQNN
                     IKGAVAPAKILSEQNKTTSILRDLL"
     gene            complement(1126..>1517)
                     /locus_tag="PLY81_10720"
     CDS             complement(1126..>1517)
                     /locus_tag="PLY81_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF30744.1"
                     /translation="KALAINPNNDSSKIGLGACYMLGNISDNPMQGIIEVKAVADKNP
                     DNLYAQWVLGLGGKKSGQYDKAAERFLFIVQKQPTNLIAMLHLAECYELNNNKAEAIK
                     WYKTVQSVIPNPEAKKELQQRIEQLQK"
BASE COUNT          471 a          263 c          196 g          587 t
ORIGIN      
        1 agtaaatctc ttaatatgct tgttgtttta ttttgttcac ttaagatttt ggcaggtgct
       61 acagctcctt taatattatt ttgaatagtc ttccaggttt ctgttaactc tagtaaatcc
      121 tgatgtagct cagcagttga tttaccttcg gcagctgttc taacaattac accaaaattt
      181 ttaggcctta tactttcaac aattttttgt aacctttttc tttcttcgcc agaatgaatt
      241 tttttagata ctgcaacaat ttcattaaat ggtgttaata ctataaaacg cccaggcagg
      301 cttatttcac aacttaatct aggacctttt gcggcaattg gctcttttaa aatttgcact
      361 aatacatttg gcttgccatt taatacttca ttaatcttac cagatttaat aatttctggt
      421 tctatctgaa aattagaaaa ttcgaagcta ttttcattag cagccattgc ttgctgggta
      481 aattttaaaa tagaacgtgc aaatggagac aaatctgtat aatgcaaaaa agcgtctttt
      541 tcaaaaccaa catctacaaa ggcagcattt agcccaggta ttaatttttt tacttttcct
      601 aaatataaat ctccaactcc aaaatttgca tcagactttt cattatgcaa ctcaaccagt
      661 tttttatctt caagcaatgc aatttctaca ccaattggcg tagtattaat tattaattct
      721 ttattcacaa aatttgcaac ttatttaaat acacaatcac tccttactta aaagaagtat
      781 tacaacagca ctatttagta attagccaga aagaaaaagg gaggaaaggt aaagttgcag
      841 caaggaaggc taatacctta aagtgtttgc tttttaaaag cctgtatata atttaaaata
      901 tacagacttt tataagcttg gcaaatgcca atcattcatt tagaagaaat attatttctt
      961 cttcttatga cgattttttc ttaaacgttt tttacgtttg tgcgtagcaa ttttatgacg
     1021 ctttctcttt ttaccacaag gcataaattc aaataatttt aaccagttaa aaaataaaat
     1081 aaattataaa acctgtattt agtttttaaa cctatacagc agtttttatt tttgcaattg
     1141 ttcaattctt tgttgaagtt cttttttagc ttcaggattg ggtataacag attgaacagt
     1201 tttataccat tttattgctt ctgctttatt gttatttaat tcataacatt cagctaaatg
     1261 caacatagct attaaattag ttggctgttt ttgtactata aataaaaatc tttctgctgc
     1321 tttatcgtat tgtccacttt tctttcctcc taaacctaat acccattgtg catacaaatt
     1381 atccggattt ttatcagcta ctgctttaac ctctataatt ccttgcattg gattgtctga
     1441 aatatttcct aacatataac aggcaccaag ccctattttg ctactatcgt tattagggtt
     1501 tattgccaat gcttttt
//
LOCUS       DAOQHP010000444         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73339_length_1517_cov_3.383037, whole genome shotgun sequence.
ACCESSION   DAOQHP010000444 DAOQHP010000000
VERSION     DAOQHP010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73339_length_1517_cov_3.383037"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLY81_10725"
     CDS             <1..225
                     /locus_tag="PLY81_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF30745.1"
                     /translation="TVQEFKGGAYPGAINIPLNTIHNQTAKFKNKNNIIVFCKSGVRS
                     AQAKSILEQSGITNVTNGGTWQSVHNAINN"
     gene            232..828
                     /locus_tag="PLY81_10730"
     CDS             232..828
                     /locus_tag="PLY81_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF30746.1"
                     /translation="MQENLNYLDKTFWNNRWLNNQTGWDIGYAAPAISNYFSQVDNKN
                     AAILIPGCGNAYEAEDLVKKGFNNITLVDIAPKAVEILQQKFINTPQVKVICTDFFEH
                     DGRYDYIVEQTFFCTFYPEVRKKYAAKAASLLNKNSKIIGLLFNKEFENNGPPFGGSE
                     LEYKKIFETEFILKTMSICTNSISPRHGSELFITLIKK"
     gene            828..1070
                     /locus_tag="PLY81_10735"
     CDS             828..1070
                     /locus_tag="PLY81_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30747.1"
                     /translation="MIQKLLTGWNFMRLLRLALGIIIIIQGILVKDWMFILMGLLFSL
                     LPIFNIGCCGATACNTNSIITSKQKEESSFEEIKNK"
     gene            1073..>1517
                     /locus_tag="PLY81_10740"
     CDS             1073..>1517
                     /locus_tag="PLY81_10740"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNF30748.1"
                     /translation="MRYLFFMPFILLAACNNHSSNQIAVSNNQYLLKKGDSIATLAQK
                     ILLQKVSNAIELGGTDYAISFCNENALKLTDTLASINNVKLSRVTDRNRNSNNALKTT
                     TDTVAWNKMKIKQASVIETNNNGEVYFYKPIVMAMPTCLKCHGKET"
BASE COUNT          584 a          214 c          253 g          466 t
ORIGIN      
        1 acggttcagg aatttaaagg aggtgcatat cccggtgcaa taaacattcc tttaaatact
       61 attcataacc aaactgcaaa atttaaaaat aaaaacaata ttattgtttt ttgtaaaagt
      121 ggcgtacgca gtgctcaggc taaaagtatt ttagagcaaa gtggtataac aaatgttacc
      181 aatggtggaa catggcaaag cgtacacaat gctataaaca attaaataat tgtgcaagaa
      241 aatttaaact atttagataa aaccttttgg aataaccgtt ggctaaacaa ccaaacaggc
      301 tgggatattg gttatgcagc ccctgcaata agtaattatt ttagccaggt agataataaa
      361 aacgctgcaa ttctaattcc aggttgtggc aatgcctacg aagcagaaga tttggtaaaa
      421 aaagggttta ataatattac gcttgtagat attgctccaa aggctgtaga aatacttcag
      481 caaaaattta taaacactcc acaggttaaa gtaatttgca ctgatttttt tgagcatgac
      541 ggcagatacg attatatagt tgaacaaact tttttttgta ccttttatcc cgaagtaaga
      601 aaaaaatatg ctgccaaagc cgcttcatta ttaaacaaaa acagtaaaat aatagggctg
      661 ctttttaata aagaatttga aaacaatggc cctccatttg gaggaagtga attggagtat
      721 aaaaaaattt ttgaaacaga atttatttta aaaacaatga gcatttgcac caatagcatt
      781 tccccaagac atggtagtga actttttata acattaataa aaaaataatg atacaaaagc
      841 ttttaactgg ttggaatttt atgcgattat taagattagc acttggtatt attattatta
      901 tacaaggcat tttagttaaa gattggatgt ttattttaat gggtttatta ttttcattac
      961 tacccatatt taatattggt tgttgtgggg caactgcctg caataccaat agtataataa
     1021 ctagtaaaca aaaagaagag tcaagttttg aagaaattaa aaataaataa acatgaggta
     1081 tttatttttt atgccattta ttttgttggc tgcttgcaat aatcactctt ccaatcaaat
     1141 agctgtatcc aataatcaat acttattaaa gaagggagat agcattgcta ctctagctca
     1201 aaaaatatta ttacaaaaag taagtaatgc tattgaatta ggaggtacag attatgccat
     1261 aagtttttgc aacgaaaatg ctttaaaatt aaccgacact ttagcatcaa taaataatgt
     1321 aaaacttagc agggttaccg atagaaacag aaatagtaac aatgcattaa aaactactac
     1381 cgatactgta gcctggaata aaatgaaaat taaacaagcc agtgttattg aaaccaacaa
     1441 caatggagaa gtttattttt ataaaccaat agtgatggcg atgcccactt gtttaaagtg
     1501 ccatggtaaa gaaactg
//
LOCUS       DAOQHP010000445         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73462_length_1516_cov_2.698152, whole genome shotgun sequence.
ACCESSION   DAOQHP010000445 DAOQHP010000000
VERSION     DAOQHP010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73462_length_1516_cov_2.698152"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLY81_10745"
     CDS             <1..950
                     /locus_tag="PLY81_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNF30749.1"
                     /translation="SNLKKMIEKNKDKFTENGFVRDGDFLFAANRHDDGEKIILGKKF
                     NSDGGYQEGIMLLKMLANHQSTAKFISKKLAVRFVCDNPSSALIDKMAKTFLLTNGNI
                     KQVILTMVSSPEFWNKDALREKVKSPFELAISSIRVLHAEVKRPFTVYNWVSNMGQKL
                     YAYQAPTGFPDNSNYWINTGTLINRMNFGLLIATNKLPGIKYNVSALNNDQEPESAEN
                     ALAVYAKILLPQRNIDATIKRLKPLLNEQNLESKLNKSKVQNVANTSNQSTMDDEEDI
                     FQNDILSTYEKENRKEEGHNKTLMQIIGIILGSPEFQRR"
     gene            962..>1516
                     /locus_tag="PLY81_10750"
     CDS             962..>1516
                     /locus_tag="PLY81_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30750.1"
                     /translation="MKTRRAFLKNTGLAMFGIGLGGVPGFVVKAAESFKQQNLYKKNK
                     ILICIFQRGAMDGLMAVTPFTDQFLKAARPNLFIEAGNNNSLIDLDGRFGLHPSLNAF
                     KKLFDEKRLAIVHGIGSPNNTRSHFDAQDYMESGTPFSKSTSSGWLNRAVGLMGHDAT
                     PFRAVSLTATLPRSFYGNNEAIAMS"
BASE COUNT          569 a          200 c          283 g          464 t
ORIGIN      
        1 taagcaattt gaaaaaaatg attgaaaaaa ataaagacaa atttactgaa aatggttttg
       61 taagagatgg agatttcttg tttgctgcaa atagacatga tgatggtgaa aaaattattt
      121 taggaaaaaa atttaattca gacggtggtt atcaagaagg aattatgctt ctaaaaatgt
      181 tggctaatca tcaatccaca gccaaattta tcagtaaaaa attagctgtt aggtttgtat
      241 gcgataatcc ttcctcagcc cttattgata aaatggctaa aacattttta ttaaccaatg
      301 gtaatattaa acaagtaata ctgaccatgg tttcatctcc tgaattttgg aataaggatg
      361 ctttaagaga aaaagtaaaa tctccttttg agttagcaat aagtagtatt agagttttac
      421 atgcagaagt taaaaggccg tttacagtgt ataattgggt gagtaatatg gggcaaaaac
      481 tatatgctta tcaggctcca actgggtttc ctgataattc aaattactgg attaatacag
      541 gaactttaat taatagaatg aactttgggt tattaattgc taccaataaa ttgccaggca
      601 ttaaatataa tgttagtgct ttaaataatg accaagaacc tgaaagtgca gaaaatgcat
      661 tagcagttta tgcaaaaatt ttgttacctc aaagaaatat tgatgccact ataaaaaggc
      721 taaaaccctt attaaatgag caaaatttag agagcaagtt aaacaaatca aaagtgcaaa
      781 atgttgcaaa caccagcaac cagagtacaa tggatgatga agaagatatt tttcaaaatg
      841 atattcttag tacttacgaa aaagaaaatc gcaaagagga gggtcataac aaaaccttaa
      901 tgcaaattat tggaattatt ttaggttctc ctgaatttca gagaagataa aagatatagt
      961 tatgaaaacg agaagagcat ttttaaaaaa tactgggtta gccatgtttg gaattgggct
     1021 tggcggtgta cctggttttg ttgtaaaagc agcagaaagt tttaagcaac aaaatttgta
     1081 caaaaaaaat aaaattttaa tttgcatttt tcaacgtggt gcaatggatg gtttaatggc
     1141 tgttacacct tttacagatc agtttttaaa agctgcacgg cctaatttat ttatagaagc
     1201 aggaaataac aattcactaa ttgatttaga tggaaggttt ggtttacacc catcattaaa
     1261 tgcatttaag aaattatttg atgaaaaaag attagctatt gtacatggca ttggcagccc
     1321 aaataataca agaagtcatt ttgatgcaca agattatatg gaaagcggaa cgccattttc
     1381 taagtcaact tcttctggct ggttaaacag agcagttggt ttgatggggc atgatgcaac
     1441 accttttaga gcagtaagcc ttacagcaac tttacccaga agtttttatg gaaataatga
     1501 agccattgca atgagt
//
LOCUS       DAOQHP010000446         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73861_length_1511_cov_6.715659, whole genome shotgun sequence.
ACCESSION   DAOQHP010000446 DAOQHP010000000
VERSION     DAOQHP010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73861_length_1511_cov_6.715659"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..927)
                     /locus_tag="PLY81_10755"
     CDS             complement(37..927)
                     /locus_tag="PLY81_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF30751.1"
                     /translation="MSISKQKATLYMHFAVLMWGFTAILGKVIHLREYILVWYRMVLV
                     VVLLLVFPKLYKELKHFSTKEIISVGLIGFLVALHWVFFYGAIKFANASVALCALALT
                     SLFTSIVEPIINKKRLNLLEFGLGLLVIPGVLLINQAIPRGFYLGWILGIVAALMAAL
                     FTSFNKKYAQNLPELGTLWLQLGAGALFLSGLLPIYIYYFPNSFVLPTNSDWFFLIVL
                     VSLCTILPYILYLKALKQSTAFATNLINNLEPIYGIVLAAVFFNENQELGGHFYLGAI
                     IILLAVFIHPIIIKFKSKHN"
     gene            1016..1372
                     /locus_tag="PLY81_10760"
     CDS             1016..1372
                     /locus_tag="PLY81_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNF30752.1"
                     /translation="MEKKYKTLKAFYPYYLTEHSDPTCRVLHYIGTSLVILVLIWAIL
                     QPVWWKFILLPLVGYSFAWVGHFKFEKNKPATFIYPLYSLASDFIMLYHFLIGKIDEK
                     LAEAKMIIAQENKNEQ"
BASE COUNT          605 a          231 c          187 g          488 t
ORIGIN      
        1 cataacaata tcaatatcaa caatacttat tcaatatcaa ttatgcttac ttttgaattt
       61 tataattata ggatgtataa atacggctaa caaaataatt attgcaccta aataaaaatg
      121 tccacccaac tcttgatttt cattaaaaaa aactgctgct aaaacaattc cataaatagg
      181 ttctaaatta ttaatcaaat tcgtagcgaa agctgtactt tgttttaaag cttttaaata
      241 taaaatataa ggtaaaattg tacataaact cactagtaca attagaaaaa accaatctga
      301 attggttggc aatacaaaac tatttggaaa ataatatata taaataggca acaatccaga
      361 taaaaataaa gctccagctc ctaattgtag ccaaagtgta cctaattcgg gtaaattttg
      421 agcatatttt ttattaaaac ttgtaaataa agcagccatt aatgctgcaa caatacccaa
      481 tatccaacct aaataaaagc ctcttggaat tgcttgatta atgagtaaaa ctccaggaat
      541 aaccaacaaa cctaaaccaa actctaataa attcaatctt ttcttattta taattggctc
      601 aacgattgaa gtaaataaag aagtcaaggc taatgcacat aatgcgacag atgcatttgc
      661 aaattttatg gctccataaa agaataccca atgtaaagca accaagaatc ctattaatcc
      721 aactgatata atttctttag tagaaaaatg ctttaactct ttatataatt tgggaaatac
      781 taatagcaaa actactacca aaaccattcg ataccatacc aaaatatatt ctcttaaatg
      841 aataactttt ccaagaattg cagtaaatcc ccacatcaat acagcaaaat gcatatacag
      901 tgtggctttt tgtttactaa ttgacataaa ttaactacaa aattgggaaa aagatatcaa
      961 cttaaagcat gaaacagatt aagattaaac tatttttcat taactttgaa ctattatgga
     1021 aaagaaatat aaaactttaa aagcgtttta tccgtactat ttaactgaac actccgaccc
     1081 aacgtgtaga gtgttgcatt atatcggaac atctttagta attttagttt taatatgggc
     1141 aattttacaa ccagtttggt ggaaatttat tttgttgcca ttggttggct atagttttgc
     1201 ttgggttggg catttcaaat ttgaaaagaa taaaccagca acttttatct atccattata
     1261 cagtttagct tcagatttca taatgcttta tcacttctta attggaaaga ttgatgaaaa
     1321 attagctgaa gccaaaatga ttatagcaca agaaaataaa aatgaacaat aataaataat
     1381 atgaacgaag tacttttatc caatcataaa ttagttgcag tttttgcagt aatattaatt
     1441 attcttatcg gaatgttatt ttacttattt tcaatagaca agaaagtaaa acaaattgaa
     1501 gaatctttaa a
//
LOCUS       DAOQHP010000447         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_73863_length_1511_cov_5.109203, whole genome shotgun sequence.
ACCESSION   DAOQHP010000447 DAOQHP010000000
VERSION     DAOQHP010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73863_length_1511_cov_5.109203"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..927)
                     /locus_tag="PLY81_10765"
     CDS             complement(37..927)
                     /locus_tag="PLY81_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF30753.1"
                     /translation="MSTSKQNATIYMHFAVLMWGFTAILGKVIHLREYILVWYRMVLV
                     VVLLLFFPKLYKELKQFSKKEIISVGLIGFLVALHWVFFYGSIKFANASVALCALALT
                     SLFTSTVEPIINRKRLNLLEFGLGLLVIPGVLLINQAIPRGYYLGWILGIVAALMAAL
                     FTSLNKKFAQNTPELGTLWLQLGAGALFLSGLLPIYIYYFPNSFVLPTSTDWLFLIVL
                     VSFCTILPYILYLKALKKSTAFATNLINNLEPIYGIVLAAVFFNENQELGGHFYLGAI
                     IILLAVFIHPIIIKYKSKHN"
     gene            1016..1372
                     /locus_tag="PLY81_10770"
     CDS             1016..1372
                     /locus_tag="PLY81_10770"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNF30754.1"
                     /translation="MEKKYKTLKAFYPYYLTEHSDPTCRVLHYIGTSLVILLLIWAIA
                     QPIWWKFVLLPLVGYSFAWIGHFKFEKNKPATFIYPLYSLASDFIMLYHFLIGKIDEK
                     LAEAKMIIAQENKNEQ"
BASE COUNT          606 a          234 c          188 g          483 t
ORIGIN      
        1 cataacaata tcaatatcaa caatacttat tcaatatcaa ttatgcttac ttttgtattt
       61 tataattata ggatgtataa atacggctaa caaaataata attgcaccta aataaaaatg
      121 tcctcctagt tcttgatttt cattaaaaaa aactgctgct aaaacaattc cataaatagg
      181 ctctagatta ttaatcaaat tagtagcaaa agcagtactt tttttcaaag cttttaaata
      241 taaaatatag ggaagaattg tacagaaact taccagaaca attaaaaaaa gccaatctgt
      301 actagttggc aatacaaaac tatttggaaa ataatatata taaattggca ataaaccaga
      361 taaaaataaa gctccagctc ctaattgtaa ccaaagtgtg cctaattctg gtgtattttg
      421 agcaaatttt ttatttaaac ttgtaaataa agccgccatt aatgccgcaa ctatacctaa
      481 tatccaaccc aaataatagc ctcttggaat tgcttgatta atgagtaata ctcccggaat
      541 aaccaataaa ccaagtccaa actctaataa atttaatctt tttctattta taataggttc
      601 aacggttgag gtaaataaag aagttaaggc taaagcacat aaagctacag aagcatttgc
      661 aaacttaata gaaccataaa aaaataccca atgcaaagcg actaaaaatc ctatcaatcc
      721 aactgaaata atttctttct tagaaaattg ctttaactct ttatataatt ttggaaaaaa
      781 taataaaaga acgactacca aaaccattct ataccaaacc aaaatatatt ctcttaaatg
      841 aatgactttc ccaagaattg cagtaaatcc ccacatcaat acggcaaaat gcatatatat
      901 tgtggcgttt tgtttgcttg ttgacataat ttaactgcaa aattgagaaa aagataacaa
      961 cttaaagcat taaacaggtt aagattaaac tatttttcat taactttgaa ctattatgga
     1021 aaagaaatat aaaactttaa aagcgtttta tccgtactat ttaactgaac actccgaccc
     1081 aacgtgtaga gtgttgcatt atatcggaac atctttagtg attttacttt taatttgggc
     1141 aattgcacag ccaatttggt ggaaatttgt tttattgcca ttggttggtt atagttttgc
     1201 ttggattgga catttcaaat ttgaaaaaaa taaaccagca acatttatct atccattata
     1261 cagtttagct tcagatttca taatgctata ccacttctta attggaaaaa ttgacgaaaa
     1321 attagctgaa gccaaaatga ttatagcaca agaaaacaaa aatgaacaat aataaataat
     1381 atgaacgaaa tacttttatc caaccataaa ttagttgcag tttttgcagt agtattaatt
     1441 attcttatcg gaatgatatt ttacttattt tcaatagaca agaaagtaaa acaaattgaa
     1501 gaatctttaa a
//
LOCUS       DAOQHP010000448         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0649
            NODE_74321_length_1506_cov_3.682977, whole genome shotgun sequence.
ACCESSION   DAOQHP010000448 DAOQHP010000000
VERSION     DAOQHP010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810281
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:16:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,118
            CDSs (with protein)               :: 2,118
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 2 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74321_length_1506_cov_3.682977"
                     /isolate="AMR_MDS_0649"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLY81_10775"
     CDS             <1..647
                     /locus_tag="PLY81_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HNF30755.1"
                     /translation="KNLNKKDANKVKELFSLPDIKGRKPYGKLETQGFRIANFMASTN
                     DEDFLVDATGNRRFLCFKVEKINDQEYSKIKIDDIWAEAYHYYRENTNNYYVTPTDIV
                     ELEENNKQFLHVTQEYEYCNLYFKKPAENDTYYLLPASIIRDYLRILTMCQIIKDRNI
                     GIALKQLNFEQVTHRFTGSSFGIKVWKVALINDKIDGKQIIEPYHAHNNNLFKP"
     gene            656..955
                     /locus_tag="PLY81_10780"
     CDS             656..955
                     /locus_tag="PLY81_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNF30756.1"
                     /translation="MPKIFKYFGYLIYIYAHDHLPVHIHAKIQDREGKFILLYNNGIL
                     TIEDQNIKGKPHLTAKEVTEMRKFVRVYANKIKEQWETIHYYKKKVTCIEVKRRV"
     gene            892..1197
                     /locus_tag="PLY81_10785"
     CDS             892..1197
                     /locus_tag="PLY81_10785"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNF30757.1"
                     /translation="MGNNTLLQKESNLHRSKTQSINYNITNAIFLHDYTLLVTFANGI
                     TKPIDFTKALEKYAQGYYQKYRQPRHFKAFKIENQNIVWGKDWDLIFPPENIFKEKF"
     gene            1265..1417
                     /locus_tag="PLY81_10790"
     CDS             1265..1417
                     /locus_tag="PLY81_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF30758.1"
                     /translation="MEYRKTETKTKGKAVAKKTAAKKPTKKGSYEAVMQGVIRAKKNP
                     IQFVWE"
BASE COUNT          634 a          235 c          234 g          403 t
ORIGIN      
        1 taaaaaacct aaacaaaaaa gatgctaaca aagtaaaaga gttgtttagt ttgccagata
       61 ttaaagggcg taaaccttat ggcaagttag aaacacaagg ctttagaata gccaacttta
      121 tggcaagcac taatgatgaa gattttttag tagatgccac aggcaacagg cgttttcttt
      181 gttttaaagt agagaaaata aatgaccagg aatacagcaa aataaaaata gatgatattt
      241 gggcagaagc ctaccactac tacagagaaa atacaaataa ttactatgtt acacctaccg
      301 atattgtaga gctggaagaa aataataaac aattccttca tgtaacgcaa gagtatgagt
      361 attgcaacct atattttaaa aagcctgccg aaaacgatac ttattattta ctacctgcat
      421 ctattataag agattattta agaatactta caatgtgcca aataattaaa gatagaaata
      481 ttggcatagc attaaaacag ttaaattttg aacaagtaac acataggttt actggcagca
      541 gctttggtat aaaagtttgg aaagtagctt taataaacga taaaatagat ggtaaacaaa
      601 ttatagagcc ataccatgca cacaacaaca atttatttaa accataaaat ttaccatgcc
      661 caaaattttt aaatatttcg gttatctgat ttacatttat gcacacgacc atttgcctgt
      721 acacatacac gccaaaatac aagacagaga aggaaaattt atattactgt acaataatgg
      781 aattttaacg atagaagacc aaaatataaa aggtaagcca caccttacgg caaaagaagt
      841 taccgaaatg cgtaaatttg tacgggtata tgccaataaa ataaaagagc aatgggaaac
      901 aatacattat tacaaaaaga aagtaacctg catagaagta aaacgcagag tataaactac
      961 aatattacaa atgctatttt tttgcatgat tataccttat tggtaacttt tgccaatggt
     1021 attacaaagc ccatagattt taccaaagca ttagaaaaat atgcacaagg ctattaccaa
     1081 aagtacaggc agccacgcca ttttaaagcc tttaaaatag aaaaccaaaa tattgtttgg
     1141 ggaaaagact gggatttaat ttttccacca gaaaacatat ttaaagaaaa attttaggac
     1201 ttataacctt ttccacttac ctcttacacc aacaaaaaaa tttatatttg taaaaacgct
     1261 ttttatggaa tacagaaaaa cagaaacaaa aaccaaaggt aaggctgttg caaaaaaaac
     1321 agccgccaaa aagcctacca aaaaaggcag ctacgaagcc gtaatgcaag gtgtaattag
     1381 agcaaaaaaa aatcctattc aatttgtatg ggagtagtta ttttagatac agatgtaatt
     1441 atagatttat accgaaataa acgtgaagca ttaaaaacgc ttgactattt taaagacaaa
     1501 gagcta
//
